LOCUS       DADXTG010000001        77417 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.001, whole genome shotgun sequence.
ACCESSION   DADXTG010000001 DADXTG010000000
VERSION     DADXTG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.001"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            85..663
                     /locus_tag="IWI57_000001"
     CDS             85..663
                     /locus_tag="IWI57_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBC2448179.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            663..1592
                     /locus_tag="IWI57_000002"
     CDS             663..1592
                     /locus_tag="IWI57_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2448180.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1589..3325
                     /locus_tag="IWI57_000003"
     CDS             1589..3325
                     /locus_tag="IWI57_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBC2448181.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3388..4224)
                     /gene="pheA"
                     /locus_tag="IWI57_000004"
     CDS             complement(3388..4224)
                     /gene="pheA"
                     /locus_tag="IWI57_000004"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBC2448182.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4221..4730)
                     /locus_tag="IWI57_000005"
     CDS             complement(4221..4730)
                     /locus_tag="IWI57_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBC2448183.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4723..6018)
                     /gene="aroA"
                     /locus_tag="IWI57_000006"
     CDS             complement(4723..6018)
                     /gene="aroA"
                     /locus_tag="IWI57_000006"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBC2448184.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6031..7122)
                     /locus_tag="IWI57_000007"
     CDS             complement(6031..7122)
                     /locus_tag="IWI57_000007"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBC2448185.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7139..8305)
                     /gene="aroC"
                     /locus_tag="IWI57_000008"
     CDS             complement(7139..8305)
                     /gene="aroC"
                     /locus_tag="IWI57_000008"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBC2448186.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8307..9371)
                     /gene="aroB"
                     /locus_tag="IWI57_000009"
     CDS             complement(8307..9371)
                     /gene="aroB"
                     /locus_tag="IWI57_000009"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBC2448187.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9404..10426)
                     /gene="aroF"
                     /locus_tag="IWI57_000010"
     CDS             complement(9404..10426)
                     /gene="aroF"
                     /locus_tag="IWI57_000010"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBC2448188.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11085..12059)
                     /locus_tag="IWI57_000011"
     CDS             complement(11085..12059)
                     /locus_tag="IWI57_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBC2448189.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(12040..13923)
                     /locus_tag="IWI57_000012"
     CDS             complement(12040..13923)
                     /locus_tag="IWI57_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBC2448190.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13941..16166)
                     /locus_tag="IWI57_000013"
     CDS             complement(13941..16166)
                     /locus_tag="IWI57_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBC2448191.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16281..17315
                     /locus_tag="IWI57_000014"
     CDS             16281..17315
                     /locus_tag="IWI57_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2448192.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17382..18395)
                     /locus_tag="IWI57_000015"
     CDS             complement(17382..18395)
                     /locus_tag="IWI57_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2448193.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18473..18661)
                     /locus_tag="IWI57_000016"
     CDS             complement(18473..18661)
                     /locus_tag="IWI57_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBC2448194.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(19101..19229)
                     /locus_tag="IWI57_000017"
     CDS             complement(19101..19229)
                     /locus_tag="IWI57_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBC2448195.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(19232..20605)
                     /gene="rlmD"
                     /locus_tag="IWI57_000018"
     CDS             complement(19232..20605)
                     /gene="rlmD"
                     /locus_tag="IWI57_000018"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBC2448196.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(20716..21771)
                     /locus_tag="IWI57_000019"
     CDS             complement(20716..21771)
                     /locus_tag="IWI57_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBC2448197.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(21781..23211)
                     /gene="gatB"
                     /locus_tag="IWI57_000020"
     CDS             complement(21781..23211)
                     /gene="gatB"
                     /locus_tag="IWI57_000020"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBC2448198.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(23208..24680)
                     /gene="gatA"
                     /locus_tag="IWI57_000021"
     CDS             complement(23208..24680)
                     /gene="gatA"
                     /locus_tag="IWI57_000021"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBC2448199.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(24680..24985)
                     /gene="gatC"
                     /locus_tag="IWI57_000022"
     CDS             complement(24680..24985)
                     /gene="gatC"
                     /locus_tag="IWI57_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBC2448200.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(25091..27127)
                     /gene="ligA"
                     /locus_tag="IWI57_000023"
     CDS             complement(25091..27127)
                     /gene="ligA"
                     /locus_tag="IWI57_000023"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBC2448201.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(27144..29378)
                     /gene="pcrA"
                     /locus_tag="IWI57_000024"
     CDS             complement(27144..29378)
                     /gene="pcrA"
                     /locus_tag="IWI57_000024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBC2448202.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            29533..31092
                     /locus_tag="IWI57_000025"
     CDS             29533..31092
                     /locus_tag="IWI57_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBC2448203.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(31203..33134)
                     /locus_tag="IWI57_000026"
     CDS             complement(31203..33134)
                     /locus_tag="IWI57_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2448204.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(33152..34066)
                     /gene="pfkB"
                     /locus_tag="IWI57_000027"
     CDS             complement(33152..34066)
                     /gene="pfkB"
                     /locus_tag="IWI57_000027"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBC2448205.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(34063..34815)
                     /locus_tag="IWI57_000028"
     CDS             complement(34063..34815)
                     /locus_tag="IWI57_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2448206.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(34974..35183)
                     /locus_tag="IWI57_000029"
     CDS             complement(34974..35183)
                     /locus_tag="IWI57_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448207.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(35194..36522)
                     /locus_tag="IWI57_000030"
     CDS             complement(35194..36522)
                     /locus_tag="IWI57_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448208.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            36658..36813
                     /locus_tag="IWI57_000031"
     CDS             36658..36813
                     /locus_tag="IWI57_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448209.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(36887..37474)
                     /locus_tag="IWI57_000032"
     CDS             complement(36887..37474)
                     /locus_tag="IWI57_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBC2448210.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(37557..38807)
                     /gene="clpX"
                     /locus_tag="IWI57_000033"
     CDS             complement(37557..38807)
                     /gene="clpX"
                     /locus_tag="IWI57_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBC2448211.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(39054..40343)
                     /locus_tag="IWI57_000034"
     CDS             complement(39054..40343)
                     /locus_tag="IWI57_000034"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBC2448212.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(40497..41429)
                     /locus_tag="IWI57_000035"
     CDS             complement(40497..41429)
                     /locus_tag="IWI57_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448213.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(41586..42245)
                     /locus_tag="IWI57_000036"
     CDS             complement(41586..42245)
                     /locus_tag="IWI57_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBC2448214.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(42248..43273)
                     /gene="sppA"
                     /locus_tag="IWI57_000037"
     CDS             complement(42248..43273)
                     /gene="sppA"
                     /locus_tag="IWI57_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBC2448215.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(43286..43576)
                     /locus_tag="IWI57_000038"
     CDS             complement(43286..43576)
                     /locus_tag="IWI57_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448216.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            43986..45791
                     /gene="glmS"
                     /locus_tag="IWI57_000039"
     CDS             43986..45791
                     /gene="glmS"
                     /locus_tag="IWI57_000039"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBC2448217.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(45863..47218)
                     /locus_tag="IWI57_000040"
     CDS             complement(45863..47218)
                     /locus_tag="IWI57_000040"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBC2448218.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(47242..48411)
                     /locus_tag="IWI57_000041"
     CDS             complement(47242..48411)
                     /locus_tag="IWI57_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448219.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(48408..49295)
                     /locus_tag="IWI57_000042"
     CDS             complement(48408..49295)
                     /locus_tag="IWI57_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBC2448220.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(49484..53164)
                     /gene="nifJ"
                     /locus_tag="IWI57_000043"
     CDS             complement(49484..53164)
                     /gene="nifJ"
                     /locus_tag="IWI57_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBC2448221.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            53362..53883
                     /locus_tag="IWI57_000044"
     CDS             53362..53883
                     /locus_tag="IWI57_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBC2448222.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(54582..55147)
                     /locus_tag="IWI57_000045"
                     /pseudo
     CDS             complement(54582..55147)
                     /locus_tag="IWI57_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(55326..55982)
                     /locus_tag="IWI57_000046"
     CDS             complement(55326..55982)
                     /locus_tag="IWI57_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448223.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(56550..57485)
                     /gene="whiA"
                     /locus_tag="IWI57_000047"
     CDS             complement(56550..57485)
                     /gene="whiA"
                     /locus_tag="IWI57_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBC2448224.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(57519..58517)
                     /locus_tag="IWI57_000048"
     CDS             complement(57519..58517)
                     /locus_tag="IWI57_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBC2448225.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(58514..59398)
                     /gene="rapZ"
                     /locus_tag="IWI57_000049"
     CDS             complement(58514..59398)
                     /gene="rapZ"
                     /locus_tag="IWI57_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBC2448226.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(59533..60264)
                     /locus_tag="IWI57_000050"
     CDS             complement(59533..60264)
                     /locus_tag="IWI57_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBC2448227.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(60268..61533)
                     /locus_tag="IWI57_000051"
     CDS             complement(60268..61533)
                     /locus_tag="IWI57_000051"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBC2448228.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(61796..64612)
                     /gene="uvrA"
                     /locus_tag="IWI57_000052"
     CDS             complement(61796..64612)
                     /gene="uvrA"
                     /locus_tag="IWI57_000052"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBC2448229.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(64622..66616)
                     /gene="uvrB"
                     /locus_tag="IWI57_000053"
     CDS             complement(64622..66616)
                     /gene="uvrB"
                     /locus_tag="IWI57_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBC2448230.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            66875..68377
                     /locus_tag="IWI57_000054"
     CDS             66875..68377
                     /locus_tag="IWI57_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBC2448231.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            68379..69116
                     /locus_tag="IWI57_000055"
     CDS             68379..69116
                     /locus_tag="IWI57_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBC2448232.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(69484..70650)
                     /gene="dnaJ"
                     /locus_tag="IWI57_000056"
     CDS             complement(69484..70650)
                     /gene="dnaJ"
                     /locus_tag="IWI57_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBC2448233.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(70801..72630)
                     /gene="dnaK"
                     /locus_tag="IWI57_000057"
     CDS             complement(70801..72630)
                     /gene="dnaK"
                     /locus_tag="IWI57_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBC2448234.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(72681..73244)
                     /gene="grpE"
                     /locus_tag="IWI57_000058"
     CDS             complement(72681..73244)
                     /gene="grpE"
                     /locus_tag="IWI57_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBC2448235.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(73338..74381)
                     /gene="hrcA"
                     /locus_tag="IWI57_000059"
     CDS             complement(73338..74381)
                     /gene="hrcA"
                     /locus_tag="IWI57_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBC2448236.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(74616..75782)
                     /locus_tag="IWI57_000060"
     CDS             complement(74616..75782)
                     /locus_tag="IWI57_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBC2448237.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(75798..76025)
                     /locus_tag="IWI57_000061"
     CDS             complement(75798..76025)
                     /locus_tag="IWI57_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBC2448238.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(76412..76668)
                     /locus_tag="IWI57_000062"
                     /pseudo
     CDS             complement(76412..76668)
                     /locus_tag="IWI57_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(77011..>77417)
                     /locus_tag="IWI57_000063"
     CDS             complement(77011..>77417)
                     /locus_tag="IWI57_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBC2448239.1"
                     /translation="HIRNLSNAKLEEEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKR
                     NPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQGGKDVIENCIALCPN
                     CHARIHELELPDDKEKLLKIVEKRENSFSKIP"
BASE COUNT        21410 a        16794 c        13683 g        25530 t
ORIGIN      
        1 tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag
       61 actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag
      121 acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata
      181 gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc
      241 gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa
      301 cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt
      361 aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat
      421 ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc
      481 ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa
      541 actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg
      601 aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat
      661 tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg
      721 gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc
      781 aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca
      841 atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa
      901 agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa
      961 gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga
     1021 atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa
     1081 tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac
     1141 tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt
     1201 tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa
     1261 aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag
     1321 aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca
     1381 tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta
     1441 aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg
     1501 gctataccga actgggttat ttctccagag tttttaaaga agagacagga atgacacctt
     1561 ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt
     1621 tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt
     1681 gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt
     1741 ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt
     1801 agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca
     1861 tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat
     1921 ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg
     1981 aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag
     2041 agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg
     2101 cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt
     2161 ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt
     2221 agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc
     2281 aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga
     2341 aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac
     2401 attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat
     2461 tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga
     2521 tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg
     2581 cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat
     2641 caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa
     2701 gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa
     2761 tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg
     2821 aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc
     2881 agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg
     2941 cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa
     3001 tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca
     3061 aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga
     3121 taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat
     3181 cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac
     3241 gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa
     3301 aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt
     3361 aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg
     3421 ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc
     3481 tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct
     3541 agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc
     3601 cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt
     3661 tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg
     3721 gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt
     3781 ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc
     3841 agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg
     3901 ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt
     3961 gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg
     4021 tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac
     4081 gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata
     4141 cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc
     4201 tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc
     4261 ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa
     4321 agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga
     4381 atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa
     4441 agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca
     4501 ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt
     4561 cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc
     4621 tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg
     4681 attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc
     4741 tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc
     4801 agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc
     4861 gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat
     4921 gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac
     4981 cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat
     5041 tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat
     5101 gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga
     5161 ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc
     5221 taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg
     5281 aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat
     5341 ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat
     5401 ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac
     5461 gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc
     5521 actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat
     5581 tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc
     5641 catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa
     5701 aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc
     5761 aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc
     5821 ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc
     5881 ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat
     5941 gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat
     6001 ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt
     6061 agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag
     6121 tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc
     6181 ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa
     6241 taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc
     6301 ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc
     6361 tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct
     6421 caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa
     6481 gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt
     6541 gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg
     6601 ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt
     6661 ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag
     6721 tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac
     6781 aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc
     6841 cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt
     6901 tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc
     6961 acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact
     7021 atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc
     7081 accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt
     7141 agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat
     7201 cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa
     7261 cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt
     7321 cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga
     7381 ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat
     7441 tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc
     7501 ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac
     7561 taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta
     7621 atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt
     7681 tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta
     7741 cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg
     7801 cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa
     7861 ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt
     7921 caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc
     7981 ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag
     8041 acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac
     8101 caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca
     8161 tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt
     8221 ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg
     8281 attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct
     8341 gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg
     8401 cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga
     8461 tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca
     8521 gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact
     8581 ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta
     8641 tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt
     8701 cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg
     8761 tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg
     8821 atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca
     8881 atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg
     8941 actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga
     9001 acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt
     9061 cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg
     9121 tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg
     9181 actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca
     9241 acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat
     9301 ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt
     9361 acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa
     9421 tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt
     9481 cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga
     9541 tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa
     9601 ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg
     9661 ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt
     9721 cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt
     9781 cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag
     9841 cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact
     9901 cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt
     9961 catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg
    10021 gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca
    10081 ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca
    10141 cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga
    10201 attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat
    10261 agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc
    10321 gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt
    10381 tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt
    10441 tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat
    10501 taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta
    10561 tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat
    10621 cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct
    10681 ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg
    10741 tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc
    10801 agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac
    10861 ctagtataga cttaaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc
    10921 tgatagttaa ctttgttttt cagaaaaata acaattggtt ttattgaaga aaatagtcat
    10981 gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga ataaagcagc
    11041 tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg aataataaag
    11101 ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc cgctccccag
    11161 ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt tcgtctttct
    11221 ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa tggtaaagcg
    11281 gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc ttgatcttta
    11341 tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc catatgcatg
    11401 ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt ataaatacct
    11461 tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc atactcaaaa
    11521 aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc cggtaatcct
    11581 ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat ttggtaggaa
    11641 attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat ttcaaccgtt
    11701 tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa atcgatcttt
    11761 ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc tcctaaccac
    11821 ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc tcgccaaaaa
    11881 actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga ttccagtccg
    11941 cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa tgtacagtcg
    12001 ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt atttttcata
    12061 ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa cgatcccatt
    12121 ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc gcatcacttc
    12181 tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc atatatagcc
    12241 tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc cacctaacga
    12301 attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt tattttcaag
    12361 gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg aaatcgaatc
    12421 ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg ggtctacttt
    12481 tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat ttccaagaga
    12541 ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa ttcgtttttg ctcgatcaag
    12601 aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct caaatgcgcc
    12661 cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat ggattcctgc
    12721 ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc gtacgccgtt
    12781 gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag tgatggcgcg
    12841 tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc gttttccatt
    12901 gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat agtcaatata
    12961 ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata aattgggctg
    13021 aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca tttgctcttt
    13081 taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg ctacattcag
    13141 ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc cattttgaca
    13201 tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc ctcgagcaac
    13261 taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt ccaagaatgc
    13321 agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca aataaggtgt
    13381 taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat gaccattcaa
    13441 ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt ttccctttag
    13501 accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt tagcagctga
    13561 aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc gaaatacatg
    13621 tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg ctaattcaat
    13681 atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt tataaaatgt
    13741 ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat ttaaagactg
    13801 tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat gatcgctatg
    13861 tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta tatttgcttt
    13921 catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt ttggaaatca
    13981 ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga aaatatccgc
    14041 ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata tacttcatgg
    14101 aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc atcttgttta
    14161 ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa acgataataa
    14221 tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc ttttacttgc
    14281 ttttcttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac agcgctcata
    14341 gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt tggtacagct
    14401 gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata ttggattttc
    14461 atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata ataaataaac
    14521 ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc cggataatca
    14581 gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc tacttctcct
    14641 tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt catatcccat
    14701 ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc aatcgtctgc
    14761 cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga aggcattcta
    14821 cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc aaccgagatc
    14881 atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata atctgctatt
    14941 tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa tgaagaattg
    15001 tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat gcctagctcc
    15061 tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta agtggcttcc
    15121 cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc gattctttca
    15181 cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga gtaagaaagg
    15241 atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa acgatggctg
    15301 ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc gaaagaatgg
    15361 ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt cgtatgatga
    15421 tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg gctttcaaat
    15481 acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac atgtgctcct
    15541 ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg gaaactagcc
    15601 gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga tcgtgtgatc
    15661 actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt ttctctatcc
    15721 ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt ttgaggcaag
    15781 ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag atccagtaag
    15841 tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat gctgccatga
    15901 gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt taagggaaca
    15961 tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta ctgcttgact
    16021 ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat cagataagaa
    16081 agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat gaaattttta
    16141 tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa accgtttact
    16201 tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt
    16261 tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct
    16321 ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta
    16381 ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat
    16441 atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag
    16501 aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc
    16561 atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag
    16621 atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt
    16681 taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata
    16741 tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg
    16801 gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga
    16861 agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc
    16921 acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac
    16981 ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg
    17041 atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag
    17101 agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga
    17161 ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc
    17221 gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta
    17281 gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc
    17341 gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca
    17401 aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat
    17461 tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat
    17521 tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca
    17581 gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat
    17641 tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa
    17701 aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg
    17761 caataagaaa tggttccttt cagactatct tttggaagta gatgtttgat tttcatttgt
    17821 ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca
    17881 ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct
    17941 aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc
    18001 gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca
    18061 aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga
    18121 acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac
    18181 ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt
    18241 tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt
    18301 ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag
    18361 aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta
    18421 agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat
    18481 tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa
    18541 acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat
    18601 ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca
    18661 tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc
    18721 tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca
    18781 ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc
    18841 gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa
    18901 ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga
    18961 atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat
    19021 cgccaaaata atgtagacta actaaaaaga ctaagaaaca ataacaaaag ttaacacttc
    19081 ttagtctttc tttagcacgt ctatttatta aagattttag ggccttttct catgttttta
    19141 cgatcattct taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc
    19201 aattctttca ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta
    19261 attcgatatg atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac
    19321 ctgcttctgc aaataactta gcatctcttg caaaagttgc cggattacat gagatataga
    19381 cgattcgttc tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc
    19441 gtggagggtc aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa
    19501 ctttttctgc ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga
    19561 actgtgcatc ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt
    19621 ctgctaaaga caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt
    19681 tatctaactc ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta
    19741 cttgataaaa tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat
    19801 agtctctgcc atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat
    19861 tgatattttg gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt
    19921 ccttggattt aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat
    19981 aatgaccacg acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc
    20041 ctcgtacttg gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag
    20101 gatcttggat ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac
    20161 cggtggctaa ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga
    20221 atggttctgc cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg
    20281 ccattacatg ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg
    20341 ctaaaggtgc gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg
    20401 attgtgtaat aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta
    20461 gtatttctac ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt
    20521 ttgctacccc cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt
    20581 tctttactat ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact
    20641 gaactctact ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc
    20701 agcaccggct tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc
    20761 gtcgatatct tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc
    20821 taattcttct tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa
    20881 gaactcaatg tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc
    20941 atctaagttg atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct
    21001 tgttttggta tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc
    21061 tgcaatatgc aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc
    21121 atcatccaat ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc
    21181 taggaaaaac atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt
    21241 gattggcttt actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa
    21301 aatactcttt agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc
    21361 gatattgatg aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt
    21421 ttttatcact tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata
    21481 gtcgttcgtt gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat
    21541 tccattaacc acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc
    21601 tagttctgcg actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata
    21661 ggcgctggct tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt
    21721 caataattct tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct
    21781 ctatcgtttc gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc
    21841 tttagttgct ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt
    21901 gaaatcatcg acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt
    21961 gttcggatca gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc
    22021 atttgtgatc aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc
    22081 gatcaaacga atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag
    22141 tttctcactg ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc
    22201 atctgcacca ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa
    22261 taccattgcg tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc
    22321 tggcatctca ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt
    22381 cgggatatca ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa
    22441 aactgtttta ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga
    22501 aagaagaact tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa
    22561 agagttcaag tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag
    22621 agaaatattg gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt
    22681 aaattggata attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat
    22741 atcagcttca gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga
    22801 ataaccgcca ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat
    22861 ccggattttt ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg
    22921 ttgatcaaat tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg
    22981 gtcaaaatga gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa
    23041 ttccagcgca gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt
    23101 tgtattgctg tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt
    23161 ggtttttaac tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc
    23221 cctcctaaaa tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc
    23281 gcttgataca tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat
    23341 ccttctgata aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc
    23401 gttaagatgt cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat
    23461 gcaactgttg gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa
    23521 tctcgtttga tcaatgtacg gacttgtcct gcttttttga agtaagcgtc ataataaccc
    23581 gcgcttaagg agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct
    23641 gaacgtgagt tgacatatac atcatctaat gatttcacat tttcagagcg ataaccgtag
    23701 cggataccat cgaatcgttg caaattggaa cttgcttctg atgaggcaat gatataataa
    23761 acagccacac cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa
    23821 cggaatgttt ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct
    23881 aagtattctt taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca
    23941 aaatcaggaa ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct
    24001 gtcaatgcta atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat
    24061 gaagcaaagg caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg
    24121 atgccgttaa aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact
    24181 ggaatttgtc cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt
    24241 gttgggtccc aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata
    24301 gcaaattcat ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc
    24361 atgaccgttg catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc
    24421 aagatatctt ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg
    24481 ctttcatcaa tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc
    24541 aatgtaatga atgcctcgac ctcaggctct gtttctttga ttcgttcaaa tgttgttttt
    24601 gtcaaatctt gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa
    24661 cgattgtgta attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt
    24721 tgataaagcc gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag
    24781 cttgcgcccg atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa
    24841 caccttccgt atctacttct tccagcattt caaccatatc gatgatttta cctaattggt
    24901 cggtaaaact tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt
    24961 tcacttgttc ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg
    25021 ctctctattt taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa
    25081 aactcatttt tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt
    25141 ctgtccaaac ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc
    25201 ctgctaccac gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt
    25261 cgatcgtctc ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg
    25321 ttttttcttt aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt
    25381 cgaaattgac gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga
    25441 aataagtgac tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact
    25501 catagtcact tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg
    25561 ctttcgctcc gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt
    25621 tgctgttgtc gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg
    25681 tcaataactc ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat
    25741 catacatctg ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa
    25801 caaagtggtt caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg
    25861 ctacttcttc atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt
    25921 ggtattcttc gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga
    25981 tgtctcccgc tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat
    26041 aatcaccgtt atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca
    26101 tgatagctgt tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta
    26161 gcgtttcaac ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgacag
    26221 taaaaccaag ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt
    26281 catagggcaa atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa
    26341 tcgaatgaca tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta
    26401 gcgcatcata ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag
    26461 tgtccagatt tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg
    26521 cgttccgagg attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga
    26581 caaatgaccg ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta
    26641 atttgattgg gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc
    26701 catccccgcg agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa
    26761 gtccatctat ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac
    26821 gttgatcaaa agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa
    26881 tatcatgagt gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag
    26941 aatcagacgt gatcaaatca ggatattgtt cctcaattgc tactaattct gcgtattctt
    27001 tatcataaac atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt
    27061 ggttcagtct tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat
    27121 gttccatcat ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga
    27181 cgttttactc cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt
    27241 actcggacga ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag
    27301 ctgttatttt cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta
    27361 tagctatatg ctgttttttt agcgaataca ggtgtttttt taggcgtagg acgcatgcct
    27421 tcgatttcca gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc
    27481 gtccgtccat ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc
    27541 gtgatcccca catatgcgag acggcgttct tcttccagtt cgctttcttc catcaatgct
    27601 cgagataaag gaaaaacccc ctcttctaga ccaatcagga atacaactgg gaattctaat
    27661 cctttggctg catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca
    27721 atatcggaaa caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca
    27781 gcgtcttctt cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat
    27841 tcctccaagt tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct
    27901 agatatccgc tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc
    27961 atctgagtga cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca
    28021 ccgatatttg ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa
    28081 gcaaaactgc gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg
    28141 atacgctcaa aactgattga atcttgagga tttgccagca cattcaaata cgctaaaata
    28201 tctttgattt ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt
    28261 gctttcaaca gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca
    28321 aagtcgccgt aattccgatg attgcttcga atctcttctt gcatccgatc tacgataaaa
    28381 cgggtttcgt ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt
    28441 tctgtccaca agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg
    28501 ctaaggatgt ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga
    28561 taatcttttt caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt
    28621 tgatctgcat ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc
    28681 gtatactgtg cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt
    28741 tgataatatg tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg
    28801 tcgtcaaagt ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt
    28861 gccgcaatct cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg
    28921 ctgttttttg cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca
    28981 atgttcagtt cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt
    29041 gtaaagttcc ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc
    29101 gagtgaaaag tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt
    29161 tctttcattt cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg
    29221 acttcttttt cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt
    29281 cctgcacctg ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg
    29341 gggttcatcc cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc
    29401 aatcttgtta attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt
    29461 gtttcttttt atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag
    29521 cgaggcgttt gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat
    29581 tttattctaa aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga
    29641 ttatctatcg aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag
    29701 ccgatttact taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat
    29761 ctggtcaaaa atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt
    29821 cgcggggaat tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta
    29881 ctttcagttg gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca
    29941 tccattggac aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta
    30001 atttctagcg gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta
    30061 ctctttgttt tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt
    30121 gcttcagcaa tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta
    30181 tatctaggtg accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga
    30241 gtacttctag gcatcttagg ttttatttat cagaaggtct tgctggcttt gccaaaattt
    30301 tacaaccgat tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg
    30361 ccaattggtt atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt
    30421 ggacatgctt ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt
    30481 tccatgattt cttacggtgg tggtcttcca ggggggattt tccttccgat attgacattg
    30541 ggggctttaa tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc
    30601 tacatcaagg attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca
    30661 ccgctgacag caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct
    30721 ttaggattgg ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt
    30781 tacgaatcat tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca
    30841 aggacaactt ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga
    30901 gacttcaatt ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc
    30961 ttgactcacg gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa
    31021 gcaaacttgc caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag
    31081 caatcatttt aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg
    31141 attctatatg attctataaa aaaagaagag actgggaaaa accagcctct tcttttttat
    31201 ttctatgctt ctgctgctgc aggagctttt ctcaataaac cataaatcac accagatacg
    31261 actgctccga tagcaataaa caccaaatac atgaccgggt gactcagtaa gaatacaacg
    31321 aaaatcccgc catgaggagc cagtaatttg atcccgaaag ctccgactaa tccgccagtt
    31381 aaagcagaac caataataaa acttggaatg gcacgaatcg ggtcagctgc tgcaaatgga
    31441 atcgatcctt ccgtgacaaa cgatagtccc atcacgatgt tcgtcaaacc agcatcttga
    31501 tctgttttgc tgaatttatt cttaaacaaa cgtgtagcaa cgaaaattgc caatggaggt
    31561 accattcctc cagccataac agaagccatc acgacacttc cgccagttgc aacagtcgta
    31621 gctaaagttg ctgtaccaaa cacataagcg gctttgttga ttggaccgcc taaatctgcc
    31681 gccatcattc cagctaataa tgcaccaagt aaagcagcat tcgaaccact catacttgtc
    31741 aagaaaccgt tcaatgcatc attgattgct ttcataggga cattgaccag caacatcaag
    31801 aaaccagtaa tcagtaagcc aaacactgga taaaagagga tcgttttaat tccatccaat
    31861 gattttggta agttcttgaa taatttacgt aaaaatacga tgacataacc agctaagaat
    31921 ccaccaatca gtgcacctaa gaatcctgca ccgcctgtat tagctaatgc accagcagca
    31981 aatccaacga tcaaaccagg gcggtcaccg atacttgaag caataaatcc tgctaatacc
    32041 ggcagcatga aaccaaatgc agcttgtccg atttggttga accagctagc agcttgattg
    32101 taattcccta gatttgataa ttgatcatgt ggcacgccaa taaattggtc gatcatgaaa
    32161 gaaagcgcaa tagcgattcc tccaccaata acgaaaggta acatgtgtga tacgccgttc
    32221 atcaaatctt tgtaaattct ttgtccgata gtaccatccg catttccttc ttctgaactg
    32281 ctttcttttc catcaccatg gaatactggt gcagtaccac taagggctag attgatcagc
    32341 tcttctggtt tacggatacc atcactgaca ggacgattga tcagttcttt tccgtcaaag
    32401 cgattcatct ctactttttt gtctgctgca acgattacac cttctgcacg ggcgatgtct
    32461 tcagcagtca atcggttttt gattccttct gaaccgttcg tttcgacttt gatctccaca
    32521 cccatttctt tcgctttttt cttcagagca tcttctgcca tgtaagtgtg agcgatccct
    32581 gttggacaag ctgtaacggc aacaacgaat tttctttctg gatgagcaga agcttctgca
    32641 gcttgtgttt cttgttctgc tttttcttct gcttcttttt gctcttcagc tgtttggaac
    32701 aagtcttgga cttcttctgg tgtatttgct tcttttaatt ttcctacgaa atctgcatca
    32761 atcagcaatc gagatagtgc tgctaatgct tgcaaatgtg tgtcgtttgc gccttctgga
    32821 gctgcgatca tgaaaaacag ataagttggc tgtccgtcta aggcttcata atctactcct
    32881 ttttggcttt ttgcgaataa aactgttgct tctttaactg cactgttttt cgcgtgaggc
    32941 atggcgattc catcaccaag acctgtagaa gtttgtgctt cgcgggctag aattccttct
    33001 ttatatgttt cgatatcgga aatccggccg gcatcaaaca ttttttggac catttcatcg
    33061 attgcgcctt ttttatcagt cgcttgcaaa tccatgatca tcgcttcttt gaccattaaa
    33121 tccttgatat tcatgattat cctccttgaa actaatcaat ttttgtgatt tcaacttctg
    33181 gcagcaactc atcaatcaat tcgcgagctg ccaagtcatc agaaaaagct gttgcgcttc
    33241 cgcaagcaac tccccattta aaggcttcga ttggatcttt cgtacggctg taagccccaa
    33301 taaagccggc aatcattgaa tcccctgcac ctactgaatt tttcaatggg cgaactaata
    33361 cattcgtttt ataagttcct tgttccgtga agagcaaagc accgtctcca gccattgaga
    33421 tcagcacatt ctgtgcccct tcagctagca attttttacc atacggaatg atttcttcta
    33481 cagattggaa ggttgtattg taaagctcag ctaattcgtg attgtttggt ttgatcaaca
    33541 atggtttctt aacaagtgca ttttttaggt catcacctgt tgtatcaatc acgaaatctg
    33601 cacctttttc tcgaatgata tcaatcagtt cttgataaaa tccttttcgc aggctagccg
    33661 gcgcgcttcc tgacaatacg acgatatctc cttgtcctac tgttcctaat gcttgcttca
    33721 actcatcgat ttcttcatct gaaatagatg ggcccaaacc gttaatttca gtttcagtat
    33781 ctgattttag tttgatattg attcttgtat cttcttttac ggttgtgaac aaagactgga
    33841 tttgttcttt ttcaagccac ttcgaaataa attcgccagt gaaccctcct aaaaacccta
    33901 gtgctgtaga ttgagaatcg atccgtttca aaatacggga aacattgatc ccttttcctc
    33961 caggtaactt gaagtcttcc ttcattcggt tcaaatcgcc tagttttaac ccatctacgc
    34021 gtacgatata atcaattgat ggattaagtg ttactgtgta aatcattgta ttgcctcctt
    34081 aattgttgtc ttttcacgga attcattaac gaattctaat ggacaacgct cggttatgat
    34141 cgtggctgct tccaaaggtg caacagaggt gaaggacaac tggttgaatt tggtatgatc
    34201 aatcaataca aatgtctctt cggaattttt catggctaaa cgtttaacag ctgcctcttc
    34261 tgggtccgga gtcgtgaatc cttgttcaag atgggcaccg ttcattccca taaatgcttt
    34321 attaaaatga aaatttttta gttgttctat cgcattagat cctaaaacag cattagttgc
    34381 cagctttatc gttccaccta aaataatggt agcaagctcc aaatcaacta aatttgccgc
    34441 atgttgaacg gaatttgtca ctactgtgat ctgttttcct tgcagaaaag gaatcatttc
    34501 taacgtagta gagccagcgt ctaaataaat gacatcttcg tcattaatgg tttcagctgc
    34561 cgatttggcg atttgctgtt tttcgtgaac gtttttgagt gatttttcac tcatattctg
    34621 ctcaaaattc aataattgct ctttcttagc tccgccatga actctttcta gaaacccact
    34681 attctccagc tcttgtaaat cacgtcttat cgttgattca gaagcattaa aaaggtttac
    34741 aagatcttgt gatttcacga ttttctgctg ttcaagcaag tctaggattt tttgtctacg
    34801 ttcctctgta agcattttca cgatcacctc aatacaaata atagcatatt ttttttctac
    34861 tcgcaatata aatcagtcaa aaacgttcat aaaaattata aatcagtcaa aaaaaaataa
    34921 aaaaccagtc aaaaagactg gttttttatt tttttgtcta tcatcaagcc gttttatcgt
    34981 tttttatttt cgcttcttgt ttttttcata taaacagaag caggttttct ttccttatga
    35041 agaagataat aataaagtcc catcccgcca aacatgacca aaaaaccgat gactgatcga
    35101 ataggaaaat taggaacaaa aaagagacag acggataata atgatgccgg aaccattatt
    35161 cgataataga gttgtttatc cattttcttc acctcatatt ttttgtaaag taatcaattg
    35221 ttctaattct cggtaatcta aaaattgcac acgcagatgc tgctcacctt tcttgacatc
    35281 gaattggata cttcgccgct ttttctttat tcgtttgaag gtaacatcag agagtctatg
    35341 tttgatttcc tttaacggta attctgacag ggattctgaa ggaacataga ttcttaagga
    35401 taaaagatac tcttgatatt tttcatctgt tacctctact tctagatatt ccctaatata
    35461 ataacaataa gcttcgattc cattatccaa agcaacagtc ggatcgataa ctttatagca
    35521 catcatgcgc gagcgattct cgtcaaccca aggaaaatac gcctgagcat ttagtgttcc
    35581 gcgaacacgc ctttctttat aatctccttg ttgaatatca taactttttg ttacaaaaaa
    35641 tgaagcattc tttttaggaa caggttgcat ttgtgcattc tgatactgga cagattggat
    35701 cgtatggtca gtgtaaatgt attcaacgaa tggcggtttc aataaaccga caccattgtc
    35761 ccaagcaaga tgatcaaacc caagctggtc cctaaaatag gattgaaaca tttgattgtt
    35821 tggcttttgc cagttaaagg ggtctcgata aagctggata catatgactt cgttttctaa
    35881 ttccttcact ttttctactt gttggatgtc gagaaaaatc ggtttaggga tgatttgatg
    35941 ataatagcga ctataataca tttcccaaca cgaagtgaaa cataaacctt ggtaaaaaag
    36001 atcgtatcct ggaaattggt tgcaatcgac tactacttcc ccgttttgtt gtttcatttt
    36061 aggtaaatgt tcgatttcat cctttttctc aaacaatagc cgttgagaga tctgttttgt
    36121 attgtgatag ataaactcgc tataagctcg catataggcg aacaccccat gagcaatcat
    36181 ttttttctga atgaagtctc gaatcaaaaa ttgcttttct ttgaaaacag agatagacaa
    36241 tacatttttt atcaataatt gattatcttc caagcgaaaa ctacattcgt tctcgtcatt
    36301 tcctacaact aaccaacttt cgtctcttac cgatgctagc attatttttt cttgaagctt
    36361 tcgaagatca tacggatccg cttgttgtgt gaaagagcgg ataaatgttg gctggatcaa
    36421 cagacgtgtc aaaatagccc aatcgcttat ttcctgtttt ttttcttctt catttaagtc
    36481 tgtgagctgg atagtcatca ataatttatc ttcgccaatc attttctcac ctctttaaaa
    36541 tctttttata atttaattat aaccaacgat aaagaaaaac taaaacttta tgtcttagaa
    36601 accaatttgg acgattacat gatagactaa gtaaaaagaa acaaagaggt gtttacaatg
    36661 gggttgaaat ttgaacgtac agataattta gataaacttt tcgaatcatt tgctgtcgat
    36721 ccggaaaaaa cagttgcacc aaaagaagag atcgatcgat ttctaaaacc acagccacaa
    36781 aatgaaaaga aaaaagacaa gaaacaaaag taagttaagc tcaaaaaaag acttaccagc
    36841 caaactttta tgaaagagtt tgactggtaa gtctttttta ttctttttat gattcaattg
    36901 cttgttcgat tgctgcccac gcttcttctt ttccctgttt tgtaacggaa gaaaagagaa
    36961 taaacgtgtc attgcgatca aaatccaacg cttttttgat ttcgctctca tgcttgttcc
    37021 atttaccacg agggatctta tctgccttag tggcaacaac aattacaggg atatcataat
    37081 acttcaaaaa ttgatacatc tggatatctt cttgagaagg cttatgacgc aaatctacta
    37141 aagaaacgac cgcacgtagc tggtctctag tcgttaaata tgtctcgatc atctttcccc
    37201 attttgctcg ctcagttttg gatacttttg catacccata tcccggaaca tcgacaaaat
    37261 gtaatgcgtt ctcgatcaaa taaaaattca atgtctgggt ctttcctggc ttaccggaag
    37321 tacgggctaa attctttcga tcaatcaatg tgttgataaa agaagatttc cctacatttg
    37381 atcgtcctgc cagagctatt tctggcaatt gtgtatctgg atattgtttt ggtgccactg
    37441 cacttatgat aatttcagct tgatggactt tcatggtctc tctccttaac aaggtgatga
    37501 ataaagcaag cggctgtcgc aaaagccaaa atgactttta cggcagcctc ttttcatcaa
    37561 cccgcttttt tctctgaatc tttatagatt actgttggct caccagacaa ttcagctgct
    37621 tcttttgtga taatcacttt ttcgatgttt tcatttgaag ggatatcaaa catcacatcc
    37681 atcatgattt cttcaatgat ggaacgaagt cctcgagcac cggtattgcg ttcgattgct
    37741 tttttcgcaa tggctctcaa cgcttcaggt tcgaattcaa gttcggtatt atctagagaa
    37801 agaagttttt gatattgttt gactagcgca tttttcggct cagtcaagat ccgtaccaga
    37861 tcatctgttg tcaatttctc taaagctgcc ataacaggta atcgtccgat aaactcaggg
    37921 atcaatccga attttagtaa atcttcagga atgatatgct gcatgacact ttcgtcttca
    37981 gaaagttttt ggttttgtgt accaaatcca atcgtctttt cgcccatacg attcttcaca
    38041 atcgtttcga tgccatcaaa tgcaccacct acgatgaaca atatgttcgt cgtatcaatc
    38101 tgaataaatt cttgatgtgg atgtttacgt cctccctgag gtggcacgct tgcaacagtt
    38161 ccttctagaa ttttcagcaa ggcttgttgg acaccttcgc cggaaacgtc acgagtaata
    38221 gatacatttt cgcttttacg cgcaatttta tcgatctcat caatatagat gatccctttt
    38281 tctgcacgtt ccacattgta atcagcagat tgaagcaatt taagcaaaat gttttctaca
    38341 tcttccccca cataaccagc ttctgtcaaa cttgtcgcat ctgcgattgc aaaaggtaca
    38401 ttcaatgttt ttgctaatgt ttgtgctaaa aatgttttac cagaccctgt aggtccgatc
    38461 aagcagatat tgcttttttg cagttcaaca tcatctgtgt tttcttgtgc ttgctgattg
    38521 acacgtttat agtgattgta tacggcaaca gctaacgagc gttttgcgcg atcttgtcca
    38581 atcacgtagt tattcaatac ttcaagaatt tctagtggtt tagggacttc agtgaattcg
    38641 cgtacagctt cttcataaaa ttcttcatca atgatttctt tacataggtc gatacattca
    38701 ttacagatgt agacccctgg accagctacg atctttttca cttcttcttg ggttttcccg
    38761 caaaaagaac agcgtacggt ctcattgcta cttggattgt catacatggt cttcacccct
    38821 taacttgcaa aaagtatgca agaaactatc aaagacttta cagtctttta ctatcataac
    38881 atagtttagt aaaaagaaaa agtgtttaac aaaggatcaa ctgacctata cctttgcatt
    38941 ttttactctt aacaaaaaat aaggaactgt gacaaaatcg tgtcacagtc ccttatttcg
    39001 aataaacaat tttctagaag tagcttcttc agaaatgatt tattacttga tttctattct
    39061 ttcgcttctg tcgctgtacc tgtgatcaat tcgattgctt ttttcattgt cacatcatgt
    39121 tccagcatat cttctgtcaa cacgcgtttg atttgttcga ttggcatgtt gtatgtttct
    39181 gataattctt ggatttctgc attgatttct tcttctgttg cttgaagatt ttcagcagct
    39241 gcaattgctt cgatcacaag atttgttctt gtgcgcattt ctgcttcacc ttcgaattgt
    39301 ttatgcaagt cttcttctgt agaaccagtt aattgatagt acatttcagg agagatacct
    39361 tgacgttgca tgttgttcaa gaattcgtcc attgaacgat gtacttcatc atgaaccatc
    39421 acgtgtggca attcaacgat ttctgcgttt tcaacagcca tacggatcgc tgcttcatct
    39481 tttgcatcgt ccgctgcttt ttcttttgtt tctactaatt ctttgcgata tttttctttc
    39541 aattcgtcta atgttgatac ttcgtcatca acatcttttg cgaattcgtc atctaattca
    39601 ggcacttctt ttgcttttac ttcatggatt gtcactttga agactgcttc ttttccagca
    39661 agatcttctg cttgataatc ttctgggaaa gttacattta catctagact ttcacctgat
    39721 ttatgaccaa ctaattgatc ttcaaaacct ggaatgaatg agcctgaacc taattcaagt
    39781 gagtaatttt cgccttttcc gccatcaaac gcttcgccat ctacaaagcc ttcgaagtcg
    39841 attactactg tatcgccatt ttcagctgca gcatcttctt tgataactaa ttcagcttgt
    39901 gcttcttgtt cacgtttgat acgtgcatcc acgtcagcat ctgttacttc acggtcttgt
    39961 ttttcaacaa ctagatcttt atattgtcct aattttactt ctggtttcac tgttacttca
    40021 gcagtgatta cccagtcttg acctttttcc atgctttcaa catcgatttt tggttgagaa
    40081 acaggatcga tgccagcttc tttgacagca gcttcataag cttctggtaa tacagcattc
    40141 aatgcatctt catataaagc ttcttcaccg tacatacggt tgaatacttg acgagaaact
    40201 ttccctttac ggaatccagg tacgttcaag ctttttttca ctttattaaa cgcagttgtt
    40261 aatccttttt ggatttcgtc ttgcgcaatc gtaaatttta aaacaccatc attcgtgcct
    40321 tttttttcaa atgttgcagt catttaattc cctccgagtt tagaaattca ttttatacat
    40381 caaaaacgtt tcatgcatac ctttaaatag tacactaacc ctttcaaatt gtaaacatat
    40441 tgaatggata gaatccttgt ttttcataac atttttatct ttttcactga taaaatctaa
    40501 tggaagaaga tcatttgctg caaaattttt tctctttttt ttaaaaaatc ttgtttttca
    40561 tcttctgtac cttcatacat cgtttttcct gtacacgcct ctacagtcag ttgaaaccag
    40621 cgcttcgggt cttcgatgta tgtatcatag acaggataaa caattgcaga ttgcaacgtg
    40681 aattcttcat ttaacacgga actaagcagc gcatcttcat taccgtaacg atctgaaaat
    40741 acacataatt gctggtaact tgcctgctgc tgcggctttg gcagatcttt tggtatgact
    40801 ttgactaact tttcttcaat cgtcaaatag aaaacaggat ggcattcgtt caattgccta
    40861 agtgtctcca gtaattttgc ccttgctaaa atatggacct ctggcgatac aataagttta
    40921 ctcgctgttt ggataaactc atgataagga agctgttcga tctcatcaat agacgcaagc
    40981 tgacgtgcag gttccaacga aggaagttcc tccgatttat tgtggagtgc cttgatcttc
    41041 tgcctctcca tttgtccgga taattcttct gcaaattgga ttttttcaat gacccgctgc
    41101 tcgaaagctt cataaaaatc tgtacttgct atcaattttc tagcatataa aaaatcttgt
    41161 gtactgatta acaagtcaaa ataaaattca gcaaactcct cattttgaag atactgtttt
    41221 tcatgcaaga gtgcttgctt caatgcttct tttttctcgt ccaactcgta aagacaaaaa
    41281 acaagcaggc tattagcctc aaagctttct ttcaactcgt aagctcgttg aaataattct
    41341 tttgcctgct gatattgctg gtttgctagt gcttgttgtg ctactcgcat taaattctca
    41401 tatttttctg gaaaatcaat cggcttcacg cagtgtcctc ctcttgctca tgttcagctc
    41461 atgttcaata gtagcaaaac aaaaaaagca tgacaattaa aaataactga agcaccctct
    41521 aaaagctaga ttttagatct gacttttaga aggtgctttc aactgttact gctttttatt
    41581 gctcatcagc tgttgacttc ctggttgaat gctttgatca gattgatcgt aacaacactt
    41641 gtattagtga aaaaatcgcc tagatgttgc tttttctgat taaagattgc tggaagatac
    41701 cctaacatca gcaaaggaaa ttttagaatg aaccggcaag caccttctct aactaaaacg
    41761 gttgaccagc taagttcttc ttcatcaaag ctgatgactc gaataccaaa gatcattttc
    41821 ccaatcgttt gaccatgatt gaatttcgtc aataaaataa aatacgctag ataaataagt
    41881 aaggctgtga atccgtagat actcagataa gaatctgaaa aattaagcca acctagattt
    41941 ttggcaatac caagtgttaa acgcgtaata gctccgatac acaaaagatc gaccaagaaa
    42001 gcaaacattc ggataaaaaa acctgcatag aaatacttag gaaaatcatt tgcttttggc
    42061 ggttcatttc ttgtgtccgt atactttttc catttacgtt gtttcttttt gatttcttcg
    42121 ggcgtcaaag gctctctttc aaaagagctc agtacctttt gggaaaagtt ggagtcaggt
    42181 cctacagcag gctgctgttg cttatctttt tcttgttcct tattttcatt tgaaagctct
    42241 gacatgctta ctcacctccg taataataca ttgcttttgg tgattccgct gtccctagat
    42301 tctcaagaac ggataagatc tgactagtct cagaaggctt caatccctgg aattctgcta
    42361 gtttacttcc taaccatgta ctagcaaacc cagatggcgt ggaagaatat tctactagtt
    42421 ccgcatcttc aagctgttgt tcctttcgca tagcttttaa agcatcttca ggaaatccca
    42481 attcatcgac taaacctact tcttttgctt ggacaccatc ataaatcctt ccatcagcaa
    42541 tttttttgac ttcttcttca gatttattcc gtccctcgct gacgattgct acaaaacgac
    42601 tatatgcgct gtcgatatat gcttgaagca ccgcttcttc ttcttttgtc tctggtcgcg
    42661 ttcctgaacc catatctttc aacgctccgc ttttaactgt tgtatcttcc acacccagtt
    42721 tttctagaag tcctgaataa ttcaatcctg acatgatcac accaattgag ccggtcactg
    42781 tttcttctgt tgcaaagatt ttatctgctt gagcagaaat atagtagcca ccgctagctg
    42841 ccatgttttt catgctgaca tacattggga tatcatgttc tttacgaatc gtatccaatt
    42901 tcttagcgat ttccgcactt tcgtagacac cgccaccagg agaatttact tctaaaaaga
    42961 tacctttgac tgttggatct tcttcaatcg ccttcagttg ttccataaaa tttttatgat
    43021 tgtaaccttc agaagcaaat aaacctgaag accctcccgc agcaatcgtt ccatctactg
    43081 ataattttac gattttttgc gtactgtctc cctcttccaa cacatttgaa gctagttctt
    43141 catccccata taaccaactg ttcaatccct caatttgatc gttttcctga ggcttagtca
    43201 aactagctga aactaacgaa accacaaata atcctgcagc aatcagaact gctgcccaac
    43261 gtctcttatt cattctttct cctttctaat acaagttttt tatgacgatc acagtttctg
    43321 actgctcaga agtgtcgaac cgctctgctt tttcttcccg cgcatacccg gaaaacgact
    43381 cttttgtcaa ttcatcttct accgtaaact cacgagcttc agcaacttcc aaggcatcag
    43441 gtgcgatttc ttggatgcta tcccagccag tatctaaaaa tattttcgta tcatctgata
    43501 caccagctga tgcttcgaat ttggacaatt gtttttttaa cattcccagt gtcatgatct
    43561 caatttttga atccattttt actcctcctc tatccgatta ctttctattg taccgaactc
    43621 ctgaaaaaga tgatagataa aaacccatta ttttttgaca taaaaagagc atttgacaaa
    43681 gcatttgata tataccaatt tattttttat tctttttttg ttgacaagaa cattcgcaaa
    43741 aggttatgat tttaaatgtt tccagataaa aaacaaaaga ggaggatagt tatacctttt
    43801 aggatagcgc caggtctgtg taaacagatg acgaggagag ggtttatcga aagattcggc
    43861 ggatggccct agggactgca ctctacagaa aatccacaaa aaatatctgc aaagataaaa
    43921 caaaaggatt ttcctgcagc tggaagcatt catacgaatt cattatagaa agtaggatgt
    43981 tttttatgtg cggaattgtt ggaatgattg gattaaaaaa tgtaacacca ggattgattg
    44041 acggtctaga aaaattagaa taccgtggtt atgattcagc tggtatcttt gtctcagatg
    44101 gcacaactga ttatctggtc aaagcacaag gcagaattca aaatttgaaa aacaaaataa
    44161 atattgatac tacaggaaat ataggtattg gtcatacacg ctgggcaact cacgggcaac
    44221 cttctgaaga aaacgcccat ccacatactt cccaaagtgg acgcttcgtg ctcgttcaca
    44281 atggcgtaat cgaaaacttt gaagaactta aaattgctta tttagcaaat gaccacttta
    44341 ttggtgagac cgatactgag attatcgctc atctaattga aactttcgcg aaggatacta
    44401 caacgcaaga ggcgttttta aaagctttgc gggtcatcaa aggttcttac gctttcgctt
    44461 tgattgatcg gacagcacct gatgttattt acgtcgctaa aaataaaagt ccactattaa
    44521 tcggactcgg cgatggattc aatgtgattg ccagcgatgc aatggctatg cttgctcata
    44581 cgaaggaatt cgttgagatt gaagatgaag agatggttac cgtcacatca gaaaaagtta
    44641 ttattcaaaa ttttgcaggt gacatcgtag aacgttcttc attcgaagca caagtagatt
    44701 cttcagatat cgagaaaggt acctatcctt tttatatgct gaaagaaatc gacgaacagc
    44761 ctattatcat gcgtcgtatc gctcaaaaat acgtaacaga agacaatcgt gtctctcttg
    44821 atcaaaagct ggtcgataca ttgtctgata gcgatcgaat ctatatcgtt gcttgcggaa
    44881 ctagttacca tgcggggtta gcaggtaaac agactctgga aaaattgaca cagatccctg
    44941 ttgaggttca tttagccagt gaatttggct ataacacacc tcttctttca aaaaaaccat
    45001 tctttatttt cttgagtcaa agtggcgaaa cagcagatag ccgccaagtt ctggtaaaaa
    45061 tcaaccgtct cggctaccct tcacttacga ttacaaatgt tgctggttct actctttcgc
    45121 gggaagcatc ctttacactg ctattgcatg ctggacccga aatcgcagta gcatcaacga
    45181 aagcttatac tgcacaaata gctgtactaa ctttattagc caaagctatc ggtgataaaa
    45241 aagaatttga aacttctctt gtattcgata tcactcatga attgagttta gttgctaatg
    45301 cgatggaatc ggtcatcgcc caaaaagatt atttagaaga gttggcagaa gtatatctca
    45361 gcgatacaag aaatgccttt tacattggac gtggcgctga ctacaacgtt tctcttgaag
    45421 ctgctttgaa attgaaagaa atcagttata tccaggcaga aggttttgcc gctggcgaat
    45481 tgaaacatgg aacgattgct ttgatcgaag aaggaacacc agtgattgga atcatcagtg
    45541 aagaagtgac tggtgcgcat actcgaggaa acctgaaaga agtcgaaagc cgtggcgcta
    45601 aaacactagt tattgtctca gagggattag aaaaggaaag tgaccagctg gtgctaccaa
    45661 ctgtccaccc ttatctaact actctaacca cagtcgtacc gactcaatta cttgcatatt
    45721 acgcaacgct attacgggga ttcgacgttg acaaaccacg taatttagcg aaatcagtga
    45781 cagtagaata ataaaagaac aaggaccagt gtcagacatc tgacactggt ccttgttctt
    45841 ttatttttta tgaaaatatc tcttaatcca gtccgatttc ttctttgact actgccgcga
    45901 ttttatcgac atagtaatta actttctcat cagtaggcgc ttctgccatc actcgaagta
    45961 atggttctgt accggaagga cgaactagga ttcttccttc tgatcccatt tcagcttctg
    46021 cctcttcaat cacttttttg attgccggaa catccatcgc accgttttta tcacttactc
    46081 ggatattcac taatttttgc ggatagattg ttacctctgc tgcaagctct gataatttct
    46141 tacctgtctg tttcatgata ttcagtaact gaattcctga aagcatccca tctccagttg
    46201 tgttaaaatc taagaaaatc atgtgcccag actgctcgcc gccaaagtta taatcgtttt
    46261 tgcgcatttc ttctacaacg taacgatcac caacttgtgt aacgacatct ttcaaaccga
    46321 ttccttccac cgctttgtgg aatcctaagt tactcatgac agtcgtaacg atcgtatctt
    46381 ttttcaatcg atttttttct gccaagtatt tagcacagat aaacatgatt ttatcaccgt
    46441 cgacaatgtt tccaagttca tccaccgcaa taatacgatc gccatcgcca tcgaaagcta
    46501 gtccagcatc tgctcctttt tcaacgacca tctcagctaa acgttctgga tgggtagagc
    46561 ctacgccatc attgatattt aacccgtctg gatttgtgcc catggtataa aaatctgttt
    46621 caagatcggc aaacaaacga ttaacacttg ttgaagttgc tccgtttgct gcatcgacac
    46681 aaatggtcaa accggaaagg tcaccgttga tcgtttgtac aaggaattga gaatatttca
    46741 ataagccttc tggaaattct tctacagttc ccaatccttc agctgatgga cgaggcaatt
    46801 catccacttc agcatctaat aatgcttcaa tttctagctc ttggtcatct actagtttaa
    46861 aaccatctgc accgaaaaat ttgattccat tatcttgtgc tggattatga gaagcagaaa
    46921 tcatgacccc tgcgcttgct ttttgcaaac gtgtcagata agcaacacct ggtgtagaga
    46981 tcacacctag ctggaacact tcgataccca ccgataaaag tcctgaaatc agcgcttctt
    47041 ctaacatttg acctgagatt cttgtatccc gccctactaa cacgcgtggg cgttttcctt
    47101 cttcttcatg ctggcttagg acataacctc cgcaccgtcc cagcttaaat gcaagttctg
    47161 gcgtaagttc cttgttggct tcaccgcgta cgccgtctgt tccaaaatat ttacccatag
    47221 ttgtaaaatt actcctttaa cttaattttc tgaggaattt gttctgctca tttgtgttga
    47281 ttcgcttgtt tctgatgttt cttctgtact tgtggtcgag ctgcttgtcc cagattccga
    47341 actggaagaa atggttgttg tcgatggttc cgttcctgaa tcagcagatg aaatacttct
    47401 actcgtactt gtattttctt gtgtccgact gctcgtcgaa gcagagctac tgcttgacgt
    47461 ttgcaacacg ggtgtaatct ccacactaac tgattcgggc ttgatactta acccctcttc
    47521 tactggtatt tcaattttac gagttgttcg atgattgata cctgagacat tgatcggtac
    47581 agcaatactg tcaccgatcg atgcaagcaa atcgctagag ccgcttactt ctgcttccag
    47641 ttttgaaaaa ctgaaatcat agctttctac cccggcagct ggtgttcctt gctgtatccc
    47701 ataaagtcgt accgacttgc ttggcttgat cgaatcagct ctcactgtta cttgcactgg
    47761 atcagagacg atacttaata cttcccctgc tgcattcagc gcctgtattg ggacctcact
    47821 attaatccca tcatctgtga ccatcgaagc gtccacatca gctactactc gtttgatttc
    47881 tgccaatgtt ttgtcacctg tagtgataga gactttttta ggactaacgg aagtatcatc
    47941 aagttcatat ccagatggtg tgaacgttga aggaatcaac gtttccacat cgaaatcttt
    48001 tattaccttt ttctcaaccg ttactgtaat agtcgatggt tcgatctttg ctgtgacagc
    48061 actgctcaaa ttttgaattt ttagcggaac ctcatgtgtt ccttcgccca gcttagtgat
    48121 atctgctgtg acgcggaaca tccgtgtatc ttcgtttgcc tctgcattca actgaactcg
    48181 gtttgcactg gaaagtttga cagtcactgc attttcatat ccatggatat agtagcgctt
    48241 tgaatcataa gaaatcttga ttggcacccc agtaactgtt tgattatacg cttcatttcc
    48301 agaaagcgta tttggaacgg actggccatt tgcattgaag tacagcgcaa tactgaacag
    48361 caatgcaata aaactgtaaa gaatgcttgt ccgtttctct tttgagatca tttgcgatcc
    48421 cctcccttag tcataccgtc taggaaagat tgaatcacgt ttttcttgtt ttttgttttc
    48481 tcttcagcaa tcagttctct acgcaaaatt gccaaatatt cttgctggga taagccagcc
    48541 atcagttcat tatctaatgt aatgctgaca tccccagttt cttcagagac aacgatggta
    48601 atcgcgtcgc tgacttcgct tataccgaca gctgcccggt gtcgagtccc gaattctttt
    48661 ggaatcaaca tactttctga tagaggaaga taggcgcttg ctactgcgat ctttccttct
    48721 tttacaataa ctgcaccgtc atgcagcggt gtattaggaa taaagatatt gatcagcagc
    48781 tctccagtga tatctgcatc taatgcaatt cctgtctcaa tatactcatc taatccagta
    48841 tgccgttcga tagtaattag cgcaccgatt tttcgcttgg acatatattg gatggctttg
    48901 tcaaaagaca agatcatctt ctcatcttcc tgctgttcgc tttttgattg tttgaaaagc
    48961 gaactgcgtc caagatgttc cagtcctctc cggatttccg gctgaaaaat cacgatagca
    49021 gctatcaccc cgtacgtaat gacttggttc atcagccaag acaaggtgtg cagaccaatg
    49081 atttccgcta cgattcggat aacaataaaa atagctacac ctttcagaag ctgaatcgct
    49141 tttgtgcctc gcagcagttg gatcagtttg tatacaagat accagacaac cagaatatca
    49201 agtatgttca ccaaaaaatt cagagagaac aagtcaccgg aaaacatttg ctgccaatag
    49261 tccaaattaa aaagttcccc gatttgaaat gacataaaaa gcctctcttc tcttttgaga
    49321 tttcctctct agtatatcat atgttttaat tttagaggct gatctgctcg tatttttctt
    49381 gagaaaaacg cataattttc aggaaaattg caagtatgcg ttttattttt ctctgcaata
    49441 aatattttta aacttcagag aattattcat tatatttttt ctgttatttt tccttttttt
    49501 cttttgtagc ttgttccatt tttttacgtc gttctgctct ggcagctctt ctttcttctg
    49561 cttcaaggtc cgtcgttttt tcttttctag tcttaacggg tacatcgata gattcctctg
    49621 gttcaagtcg tttcttgatt ttttctaatt ctcccgtcag tgcagcatag ttgtaaaaac
    49681 gtgttttagc gtcttggata gtcttttcaa ataaagcttt tgcaagattt ggatttgcgt
    49741 tctctagcga ggaaaagcga acttgtttcc tcatgaaatc aggcatagca gagaaatcag
    49801 gttttttgta gtccaaagtc attgggttct tgtttttttc gaccattgca gggttatatc
    49861 gatataatga ccaatagcca gaagaaacag cttcttttgc ttctttaagc gattctttca
    49921 ttccaccaag caaaccatgt gtgatacaag gcgtataggc aatgattaaa gaaggtccag
    49981 gatacttttc tgcttcttca aaagccttta tcgtctgcat ttgattggca cctgaagcaa
    50041 tttgcgccac ataaacattt ccatatgtca tcgccatcat tcctagatct ttctttgaag
    50101 catactttcc actagccgaa aatttagcga ttgccgaagc tggtgttgct tttgaggttt
    50161 gaccgccagt attggaatag acttcgttat ccaaaacaag aatattcacg tctgctccgc
    50221 tggcaagtac atgatcgatc ccgccaaaac caatgtcgta agcccatccg tctccaccaa
    50281 tcatccattg gctgtttttt acgaatagat cctttctctt gtatatatcc tccagttcag
    50341 ggacaattga tcgttcgctt tcgatcgcag cttgcagttt tgctgctctt tgctgcgttc
    50401 cttcgctctc aaacatgtgt accaaccagt cttccatcaa ttctcgtagt tcttctgata
    50461 cttttggcaa tacttctttg attgaacttg ccaaagcctc gcgacgagtc tgtgccgcta
    50521 gaagcatacc ataaccaaat tcagcattat cttccaataa agaatttgac catgcaggcc
    50581 cttgaccaca ttcattggtc gtataaggag aagcaggaga agcaccgccc caaatagaag
    50641 aacagcctgt cgcattcgcg atcatcattc gatcaccgaa catttgtgtc aataatttta
    50701 cataaggtgt ttcgccacag cctgagcaag cgcccgagaa ttcaagaagc ggttgattga
    50761 attgtgtaga caagacagta ttctttcctt tgactggatt tgctttttgt cttaatgtca
    50821 ttgcaaaagc ccaattcaca gcttgttctt tctgttcttc ataaggcttc atcactaaag
    50881 ctttttcttt tgcgggacaa gcctctacac acagaccaca acctgtacag tcttccactg
    50941 aaacttggat acggtatttt acgccgtctg cgcctcgcat ttctctcacg ataaatcctt
    51001 ctggcgcttc ttccatttcc tcttcatctg ctaaaaatgg acggatggca gcatgaggac
    51061 aaacaaaggc acattcatta cacatcgtac agcgatcact aatccactca ggtacttcta
    51121 aagcgacgcc ccgcttttcc atagaagccg ttcctaaagg catttcgcca gttgtcattc
    51181 catttgctac aagtgtacca acagagagtt gatttccttc ctgtgcattc actggttcaa
    51241 gtatttcttg tatataacca gtgttccttt tattgatttt ttcaggcatc tccaacgttc
    51301 tccaatggtc tgggatcgcg acttgatgaa gcagttcggt cgtccgatcg attgcttgta
    51361 tgtttttttc tacgatagcc attgatttat gtccatagct tttcagggct tcttctttta
    51421 gcagcggaag cacctcatca aaaggaatga tgtcagctaa tttaaaaaaa gccgtttcca
    51481 ttgccgtatt gatccggcgt cccaaaccga cttcttgggc caatctcatg gcattgatcg
    51541 tatagaatct aatgttattt tcagccaagt aacgttttaa ttttaagggc agatgcgttt
    51601 ctaattgttc gtcgctccaa agagtattca ataaaaaaat gccacctggc ttcaatcctt
    51661 tgaccaagtc atagctatgc aggtaagctg gtgtgtgaca ggcaacaagg tccgcatgct
    51721 cgacaagata cgctgaacgt atcggcgtat ctccaaaacg aagatgagag acagtcagac
    51781 cgccagattt tttcgaatca taataaaaat agccttgcgc atatttatcg gtgtgatctc
    51841 caataatctt gatagctgat ttatttgctc caactgttcc gtcagatcca aatccccaaa
    51901 acttggcctg gaatgtttgc ggttctgtca aatccagact ttcctttggt tccaaagatt
    51961 gataagtcac atcatccacg atccctatgg taaaccgttt tttgcgtatg cttgggtctt
    52021 ttttcaattc atcaaaaact gctgagattt ggtcgggagt cacatcttta gaccccaatc
    52081 cgtagcgacc accaataaca gccgggcgaa gttcagaatc atataacgca ctttgaacgt
    52141 ctaataagag aggttctcca cctgcccctg gttctttact tcgatcaaga acagcaattg
    52201 tttttactgt atgaggcaac ttttgcacga atgtttccac tggaaaagga cgataaagat
    52261 ggatattcaa aaaacctgtt ttccgtccgt ttgcattcaa gtaatcaatc gtttgttcaa
    52321 tcgtctgtgc aactgatccc atagaaacaa tgacttcttc cgcatcgtct gctccgtaat
    52381 aagtcaccaa gtcataagaa gttcctcgta atcggttgat ctctttcata tatttttgaa
    52441 cgatttcagg taatttatca taataggggt tgatcgtttc ccgttgttga aaatggatgt
    52501 ctggattctg gttcgtacca ctaacagtcg gatgattggg attcatccct cgtttacgaa
    52561 atgaagctaa tttctcctga ttgatcagag gagctaattc ttcatattcc aaaacttcga
    52621 ttttttgaat ctcgtgactc gtacggaatc catcaaagaa attcaaaaaa ggtacttctc
    52681 cttcaataga agcaagatgg gcaacaggtg ctaaatccat cacttcttgt acactgcttt
    52741 cagctaacat agcaaatcct gtttgtcttg cagccatcac gtctccgtga tctccgaaaa
    52801 tattcaacgc atttgtcgtc actgcccgac tagcaacatg gaaaacactt ggcagtagtt
    52861 ccccagcaat cttgtacata ttcggtatca tcaaaagcag cccttgagaa gcggtatatg
    52921 tagttgtcag tgcaccagtt ttcaatgatc catggaccac accagctgct cctgcttcgg
    52981 attgcatttc tactactttt actggttgac cgaatacatt ttcttttcct gctgctgacc
    53041 attgatctac taattcagcc atggtcgaac tgggtgttat tggatatatt gcagcaatct
    53101 cggtaaatgc ataagaaata tatgcggctg ccgtattgcc atccattgtt ttcatttttc
    53161 gcatgattca tcatccttgt tcatattctc tcttattatc tgttccatta taacgtaaaa
    53221 cattccattt tttcttttat ttataaaaat atttcacata tgtctatttg aattggtcat
    53281 ctaattttta gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat
    53341 aagcgaaaga ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca
    53401 caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac
    53461 ctatcttgtc cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca
    53521 gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagcttg
    53581 cagcgaggaa acgtgatgca ccggtatctc acaatcagcg atggtcaaaa agcgatcaaa
    53641 gagatcaaac aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta
    53701 gcggattttg cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa
    53761 gctgtcatca acgaagctga gcaagcacat attcgatttg ttccgactgt catcttgggt
    53821 aaagaaatct ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa
    53881 taaaaaaata aggagcgtag gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    53941 ctataaacgg cggaagcaga agcaactcct tcttatttct tggagttaaa cgcttctgtc
    54001 ccagcctcct tattccgaat aaatggtgtc caaaagcttg catctgccag attatctcat
    54061 ttcttatgac aagctgagca aattatctcc accaaataat ttattcatat ttttgacttg
    54121 cgcatactaa ctaattctta aattttcata caaaatttct caaattccct taacgagtaa
    54181 tatttatgta cggtgattca cactagtatc ccaatcactt tttaagttat cccgatttca
    54241 attctacttc aaaaatagtt gcttttatgt atgctgttag ttgattagat ggtcgtttgt
    54301 tgagctaggt cgtttatttt aaacaagttg tttctttagc ttatacctat ctcgtctacc
    54361 aaacgcaaga tggcaaacac aaatgcatac gcatctgtat ttgccacttt gattcgtata
    54421 aagctaacaa ctcctaactc ctctcattta atagttgctc tcttttttat tcagatacat
    54481 ttttgctgat cacagcctag tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt
    54541 acaacattca ctggctgatg aaatggatta atagatttcg attattcaaa gcgattttct
    54601 attggtggat tgtgttccca actaacaaga cggattccaa agtcattata taactcttca
    54661 agagttaacc ctttcctgtt cccaatagat gtatctctcc cacgctcgat ataaaatatg
    54721 gagtttcgaa tgccaagacc tggcaaatct ttccttgtta cagttacact gctactaact
    54781 tccccatata aaatccagct cctccaaaat gataatatat ttttcgacca tcatctaact
    54841 catgtaacaa taattgttgt gccctcttgt cactcgccaa atgcaaacgc cacgtgcgat
    54901 accatgccat gctacctgca tctgcagaaa atccatcaaa taatgcaaca attgtatctt
    54961 ctatcacaat cctttcccca tctacttcat attctaatct aaaatcaaac tcccctcggg
    55021 taatcgcagg tcttggtggc ttaggaccca atattattct acttaaaacc gcattcgttc
    55081 ccagatgaaa cacagctata acaacaataa tgattaatat tttgtttcta tttgataaat
    55141 ttctcattat tcgcaaatca cctctacatt ctcacatact agttcgtaat actcctttaa
    55201 tatcaaaata tctactgatc gtaataatat tcgctatgaa ttcctactac tcgtataaga
    55261 aaggccaaat catcagcttt tacttgctga tgatttggct tgtttctatt tatcgtaatt
    55321 gttttctaat caccaaaatt gttaacaatc ggttcagata tttcccaatt cactagcctt
    55381 atattatatt gatttaacaa ttcttcttca tttattgtat tttgatcaag cggaccaaat
    55441 tgttctaata attctttgtc atgtaaaata tcccttctag taatgcctct ttccctttca
    55501 atgaaagaag cactaggaaa agtatgatta taaccattca acctatctcc catgtaataa
    55561 ttagcaggtc ctggaaaata aaatatttgc ttattatcac taacttctaa cagcaacact
    55621 aaatcttctc cgctggctaa acgacttgtc catctacgat atctccctat gccttcgttc
    55681 attccaaatc catcaaattc aactattaac gtgtcttcta tcacatgtaa ttcttcattc
    55741 aattcatact caagtctaaa aggaaattct ccatatgtaa tctctggcaa tggtggtgct
    55801 ggagttctcc aaataaatac aataactgtg aatagaaaaa atccaacgat cgatataaat
    55861 gttatgagac ctgctcgaag taaaacccga tataataaaa atgctgtatt tcgctttgct
    55921 atatctagaa aaataaaaag tactgcaatc ataatgatag taaaagttgt aaaaaaacgc
    55981 atttttgccc tccctttata aacatcgatt ctcaaattct aaaccaaatc ttggtagttc
    56041 aagttcaggt ataacgtatt ccaaccatac atctcaatta ctatttatga acttatgatt
    56101 atcactgaat aaatcactcc atccccttgt taaaacttct acattaatta cacctggttg
    56161 ttttcgacta gctaatccaa tgaacttatc aaccatcctt atccaccaac cacgagaatc
    56221 ttgaccagaa gtaagaagag cagttctcaa ggatgatcga gcagttcctt ccagtttttt
    56281 aattaatcag gccagttata gataaatact tgatcttatc tatatcagga cttctatatt
    56341 aagcaaaaat ttatgtacaa aactaaaatc ttcattcagt tgtctccccg ctttttatac
    56401 gattgtatta aaaacaatag aagcctttcc aaattatgtt actccagtaa agatggaatt
    56461 atagtttgac ggaagaaaag caagtataac ttttaaaaaa aactgataca ttcgcatctc
    56521 ctgcttcatg tatcagtttt ttctttggat taaactgctt cttcacgcag tttttccgca
    56581 aattcattta ttttgcgaat tcggtgatta attcccgatt ttgatatcgc acctgaggga
    56641 atcatttccc ctaattcttt caagctgacc tctggatgtt gaagccgaag ttctgcgatt
    56701 tcctgaagtt tttcaggaag ggcatgtaga ccgacctttg cttcgatcaa ttcgatgttt
    56761 tcgatttgtt tagaagccgc atcaatcgtc ttgttcagat ttgctgtctc acaattgact
    56821 agacgattga ccgaatttct catatcccgt acgattcgaa catcttcaaa tcggagcatt
    56881 gagttggttg caccaatcaa tgttaagaaa tcagcaattt tttctgctcc ttttaaataa
    56941 gaaatatagc cattgcgccg ttcaagcgtt cgagcatgta gaccgtaata atttaacata
    57001 tcgcaaatgt cttggttatg ctgttcataa atagaaaata tttccaaatg ataacggctc
    57061 gtttccggat tattgactga acctgaggcc ataaaagcac cacgtaagta agaacgcatt
    57121 ttttgcgaat ttcccataat ctcgtccgaa acgtgtccat tgaacatcat accatccatg
    57181 atatcaagat ccatcaacac atcttttgtg ccttgcttca accggacgat atagacattg
    57241 ttctttttta atttcatttt tttacgaacg agtagctcac ttcgtacctg ataatgatct
    57301 ttcaaaagag aatagatacg tctggcaatt gcagcatttt ctgtttggac gttcaaaata
    57361 aactgctggt tcacaatacc taaagagccg ttcattcgca gcaaagcagc taattccgct
    57421 ttcgcatgtt ctcgatgaac ttccaaacca gttagttctt tcttcacatc tgcagcaaat
    57481 gacatcctat tctcctcctt tctccaacaa taaattgctt aatattttga accgaaaact
    57541 aaacggaaca attcttctac tactttgtct ccatcgtgga agacaccacc atctttcaat
    57601 tccaaaaagt cggtggaaat cactcgacat ccttcatttc tcaatccgtt gaagtcgtgt
    57661 gcgacttgta ctagatactc atcatagatc tctgggtcca tataatcttc aggcactttt
    57721 tccgtattga ccaatactgt atcgataaaa ggctgattca aatgcttgtg taaaacacgt
    57781 atatgatcgg catctgtgaa atgttccgtt tccccttttt gggtcatgat attacagata
    57841 tagacggttt cagcagttgt cttgagcatt gcttcgccga tttctggtat cactaaattt
    57901 ggaaggatac tggtgaataa acttccgggt cccaaaacga ccatgtcggc gtccataatt
    57961 gctgaaacga cttttcgagc cgctttggct tcacttttat catttgtgtt gcagacatac
    58021 acacggtcaa tggttttacg gtcgatcgcg attttagatt ctcctactgc agtcgttccg
    58081 tctttaaaga cagcatgcaa ggtcaaagga gtttctgaag aaggataaac atgtccatct
    58141 acatgcatca tttttgctaa taattgaatc gcctcataag tgcttccccg catctcggaa
    58201 actgcggcaa tgatcagatt accgatcgta tgattggcta aaaactgatc ttcctttttg
    58261 aaacggtatt ggaaaatatc ttcatagaat tgtggcatat ccgataacgc cactaagaca
    58321 tttctcaagt caccaggagg agccatagcg atcgattctc tgattgcacc gctgcttccc
    58381 ccatcatctg caacagtcac tactgctgtg atatctgctc cttgatttcg caaacttttc
    58441 aggataactg gcagaccagt gccaccgccg accactacga tctttggttt tcttatccgg
    58501 taggttttca ttttcacgac cggttcaccg tctctttcct tttatccttg tcccgatgtg
    58561 tcacattgac tttatagttt ttccctaaag catttgccaa acgttcagtc aatgccacag
    58621 aacggtgttg accgcctgta cagccgattg caactgtcaa gctgcttttt ccttctttaa
    58681 tgtatccagg caatacagtt tccagaagat ccaaaaattt atggtagaat gattgcgttt
    58741 catcaaagga catcacataa tcatagactg gctgatcttt tccggtcaat ggtcttagtt
    58801 ccgggatata atgagggttg ggcaaaaaac gcacatccat cacaatatcc gaatcgatcg
    58861 gcaacccgta tttaaaacca aatgatacaa attcgatccg aaatccttgg tctccctttg
    58921 atttgaatga ctcattgatt ttttcacgta attgtcgtgg tgtcaatgtc gtcgtatcaa
    58981 tgatcacgga agcttgggct ttcaagtcgt ctagtatcgc ccgttcttta cgtatccctt
    59041 cagtgatcaa accgtccatc gccattggat gtgctcgtct tgtttctttg tagcgagcca
    59101 ctagttcttc gtctgaagca tctaaaaata agatagaagt gtcgatgaag ttcgtatttt
    59161 cgatttcgat cagcatgttt tggatttctt caaagaagga acgagaacgt aaatccactc
    59221 ctaaagccat ttttgtgact ttgcctgatt ctttgattaa ttcccagaat tttggaatca
    59281 atcgaggcgg catattatcg atacaaaaat agcccatatc ttcaaaactt tggatagcta
    59341 ctgttttccc tgcaccgctc atacctgtta tgatgactaa ttgcaaatta tctgtcataa
    59401 ttacgaactc ctctcacatt gtgcatagta aaattatatc attccgggcg tatcattacc
    59461 agttttcttt ttcttttaca aaaagatttg gaccaaatat cttttaggat atttgcccca
    59521 aatccaattt ttttactttt ccgatgctgt atccaatgcc ttgcttcttt cagctagcgc
    59581 tcgttcaatc gtccgatctg ctaaaataga ttgggcatcg atcactggat aatggttatc
    59641 ttcagcaaat tcatgcatca atgacaattc tgtgcaacct aagataactt tttcacagtt
    59701 taggcgctct accgcttctt ctaagatttc gtaatatagc tcttgattca aataatccga
    59761 ttccttgatc tcatgataga tcagataatt gattttttct tgaagaacag tatcaggaat
    59821 cactgtttcg aatccaagat ttttgacttc gcgttcatag attccagctt tcatacttcc
    59881 ttctgttcct agaatcgcta ctctccctgt cgtatgctga cggacaagtt catttgctgc
    59941 ttctcttggc atatgaagga taggaatatc cgttgccgct tgtaattctt caaaaaagta
    60001 gtgtgcggtg ttacaagtca acacaatgaa gttcggctgc aataaatttt gtttctcaat
    60061 atcatccagc agaaacggca ttggattttc ttctgatcga tctaaaatat aagctgtacg
    60121 atcaggaact gttgcatgat tgaataagac ataattcaaa tattcttgat cttttgtagc
    60181 tttcgttcgg tggttgatca agcgaacaaa actttctgtc gccatcgtgc ccattccgcc
    60241 taaaatactg aaaaaattct ccatgaacta gccttctttc acatgaaaat actttttgaa
    60301 tcttggaata tagttatgga acatatattt catcaacaac cagcgcttga ttgaccggtc
    60361 tttttcataa aacacagtag taccgtaacg attttctttg atcatttgct cagctagttt
    60421 tttgtcttca ttttcccgag catattctaa aaagattttt ttgggcacac ccatccataa
    60481 tcttgcttta tctgattgat tcgttccata ggtcgtttca acaaagggtt tgttaaacac
    60541 tcgatcttct gtgacgaaac gagccagatt caagccgttc aatgtcacaa agaaactgct
    60601 acgaccttgt ctcaaattga tttcaaataa tttatattct ccatctctag gatcatactt
    60661 catatcgaaa ttggcaaagc ctgtatattc gattttttcc aaaaatgcct taatggtttg
    60721 atagattttt tcgttataat ctggcatgat cacaacgtaa ttaccaatcg atgctggtgt
    60781 aggatcttct aacagtggat gaccaagaca catcatccga acttgatgat cttcatctac
    60841 atacgcattt agcacacgca tattactatc atcacccggg ataaaatctt gtacaatcat
    60901 ttcactttta taaccggctt cgtatatccg gcctaaaatt aaatcgaact cttctctagt
    60961 ctctaaaata aaagcttttt ttcttccttc aaattggaca gaaagatact ccacactgtt
    61021 tgcaggtttc aaagcaactg ggaattcaaa aggcaattcc tgttccaaat gtccatttac
    61081 taacatttct tctctgacga tcaatgtttt aggatacgga agatcatatt cttcacaaac
    61141 ttcataaaag cttactttat tgatcagtct ttcaaataaa gaataatcaa tatatggaca
    61201 gataaaatat tctgacagct cttgtttatg ttgggagatc aattctgcat atccatcacc
    61261 acaagcaatc aaaagatatt ttttactctt atcagaatat ctttcttttg ccaaacgaag
    61321 catcgtctcg ataaacacag ggtctttatc aaatcctgga ataacttcta cattgacaat
    61381 tttactgtag cgggtagggg ctaactgatc cgaagcatat gcttggcaga ttttgccgta
    61441 tgcttcatta aaagaacgtg ccataccata aacgttcatg tcgcttccta ataatatagg
    61501 gataaattct tcattttcaa tactgttcat cattatctct cacttactca taatctcttg
    61561 tttttctttt ccaactacac aaggaatatc ataccataaa gaaaaaagca atcaaaggaa
    61621 actcctccga ttgctcaatt cttttttgaa cggattgaac ttataggaag tttttgtcta
    61681 taccaatcag tgtagaaaga actaccatgt aatgattcta gaacactgat attaataaaa
    61741 caagcaaaaa ttcatttttt tatgtttttt taactatctc agctatctat cctctttata
    61801 ccattatttt tttcaaatag tgacctgtat aactttcttt tacgttaatg atttcttctg
    61861 gtgttccagt cgcaaggatc gttcctccac cttctccacc ttctggtcct aaatcaatga
    61921 tataatctgc tgttttgatt acatctagat tgtgttcaat cactaaaact gtgttaccag
    61981 catctactaa tctttgtaat acatgcaaca agcgggcgat gtcatcgcta tgaagtcctg
    62041 tcgttggttc atctagtata tagaaattct ttccattaga gattttgtgc aattcactgg
    62101 caagtttcat ccgttgtgcc tcaccaccgg acaatgtcgt tgctggttgc cccatcgtca
    62161 cataacctaa gccaacatca acaatcgttt gcagtttgcg atgaatcttt ggaatgtgct
    62221 tgaagaattc tacagcatct tctactgtca tttccaaaat atcagcaatg ctttttcctt
    62281 tgtaatgcac ttctaatgtt tcagagttat atcgtttgcc atgacatact tcacaaggaa
    62341 catagacatc aggcaaaaag tgcatttcga tcttaataat tccatccccg cgacaagctt
    62401 cacaacgacc gccttttacg ttgaaactaa aacgcccttt cttataaccc cgcatttttg
    62461 cctcgttcgt ttgagcaaat aacccacgga tatcatcaaa tacacttgta taagtcgctg
    62521 gattacttct cggcgtccgg ccgattgggc tttgatcgat atcgatgatc ttttcgatac
    62581 tttcgtagcc ggaaattgtc ttgaatttac ctggcttagc agaattctta tttaattttt
    62641 gcgctaacga tttctttaag atactattga ccaatgtact ctttccagaa ccagacacac
    62701 ctgtgactgc tacaaattct cctaatggaa attcaacact aatatttttc agattgtttt
    62761 ctgaagcacc agtgatcttg attgctttcc cattaccttt acggcgttct tttggtacag
    62821 ggattgattt tttccctgaa agatatttcc ctgtcaaaga attttcatca ttagctactt
    62881 cttcaggagt gcctgcagcc acgatttgtc cgccttgttc tccggcccca ggtccgacgt
    62941 caatcagata atctgctgcc cacatcgtat cttcatcatg ttcgactaca acgagggtgt
    63001 tgccaagatc acgcattttc tttaatgagc caatcagtcg atcgttgtcg cgttgatgca
    63061 atccgatcga tggttcatct aaaatataca aaactccaga aagattcgat ccaatttgtg
    63121 tagccaatct gattcgctgc gcttctcccc cagataatgt tccggaagcg cgactcaaag
    63181 ttagatagtc tagtcccaca ttttccaaga atgaaagtct gtcatttact tcttttaaaa
    63241 tcggtttggc aattactttc tcttgttcag ataacgttaa gtttgagaag aaatctactg
    63301 ctttttgaat agacaactca tttgtttgcc cgatattcgt tccattgatt tgaacagata
    63361 gagcttgggg attcaatcga taacctttac atgtctgaca agttaattct gtcatataaa
    63421 gacgcatttg ctcacgcgtg aaatcactgt tcgtatcatg ataacgacgc tcgatattct
    63481 tcaatacccc ttcgaaagtc gtttctacat cgcgaacgcc accaaaatca ttttcataat
    63541 ggaaatggaa aagatcatct gtcccattca agatgatatc ctgatgctct tccggtaatt
    63601 cttcgaatgg tgtatctaaa tcgataccga aactttcaca agcctgcgct aacatttgag
    63661 gataatactg agagctgatt ggattccatg gaacgatagc accttgacta agtgtcttcg
    63721 ttctatctgg aatcactaaa tcttgatcga cttctaattt gacacctaaa ccatcacagt
    63781 ctggacaagc tccaaaaggc gcattgaatg agaacagtct tggttctagt tcgccaacag
    63841 taaatccgca ataaggacag gcatagtgtt cgctaaacaa catttcttct tcaccgatca
    63901 cgtcaaccag agcatagccg tctgccaatc gcagagccgc ctctaaagaa tcaaatagac
    63961 gagaacgaat cccttctttc acaatgatcc ggtcgatgac gatcgcgata tcatgttttt
    64021 tgtttttctc cagctcaggc acttcactta aatcatagat ctcaccatcg actcgaacgc
    64081 ggacgtatcc ctctttttgg attcgttcga atattttttt atgttgtcct tttttcttta
    64141 ccacgatcgg tgccaacact tggatctttg tgcgctcagg caaagccatt acttgatctg
    64201 ccatttgatc tactgattga ctggtaatct cgatatgatc gttgggacaa atcggatgtc
    64261 ctacacgtgc aaacaacaag cgtaaatagt cattgatttc cgtcacagta cctactgtcg
    64321 accgaggatt tttacttgtt gttttctgat caatagaaat cgccggactc aatccgtcga
    64381 tgctatctac gtccggctta tccatttgtc ctaaaaactg acgagcataa gcagaaaggc
    64441 tctctacgta tctccgctgc ccttcagcgt acagcgtatc aaatgctagg gaactttttc
    64501 cagaaccgga caaacctgta acgaccacga atttatctcg tggaatcgtt acatctatat
    64561 ttttcaagtt atgggcacgt gcaccgtgaa tcgtaatttt atcgttagcc atgttgtcct
    64621 cctactttga agcttttaat tctaaaatca tatctcgcac attagcggct gtttcaaaat
    64681 ccaatgcttt cgccgcattt ttcatttctt tttctaattt catcaataac gtatcttttt
    64741 cttctttcgt catttcttca tacgagactt gtaccgcttc ttttgtatca tcttccgatt
    64801 ctttcgtaat agaaatcaaa tctcggattt ctttaatgat cgtttttgga acgatcccat
    64861 gttcttcatt gtacttctgc tgaatcgtac ggcgtcttga tgtttcatcc atcgctagac
    64921 gcatagaatc tgttactttg tctgcgtaca tgattacttt accttcttca ttccgagcag
    64981 ctcgtccaat cgtttggact aaggaacgtt cactacgtaa aaagccttct ttatccgcat
    65041 ccaagatagc aacaagggaa acttcaggta cgtctaatcc ttcacgaagc aaattgattc
    65101 caactaacac atcaaattca cctaatcgaa gatctcgaat gatttctgtt cgttccagcg
    65161 ttttgatatc actatgcaaa tatttgactt tgatccctaa ttctttgaaa taatctgtga
    65221 ggtcttctgc catttttttc gtcaatgtcg ttacaaacac ccgctggtct ttttcgactc
    65281 gttcatttat ttcgcctact agatcatcga tttgtcccat gattggacga acttcgatca
    65341 ctgggtcaag cagccctgtc ggacggatga tttgttggat aacggtatct gtttgttcat
    65401 gttcatatgg tcctggcgtc gcagagacat aaatgatctg attgacatgt ttttcaaact
    65461 cttctaaccg aagtggccga ttgtctaaag cagatggcaa acggaaaccg taatctacta
    65521 gcatctgttt tctcgctcgg tcaccattat acattcctct gacttgaggc atggttacgt
    65581 gcgattcgtc cgccacgatc aaaaaatctt caggaaagaa atccaatagc gtataaggcg
    65641 gttcaccttc tttacgtccg tccatatgcc gagaataatt ttcaattccc gacgtgtagc
    65701 ccatttctaa catcatttca atatcgtaat tggttcgctg ttctaaacgt tgagcttcca
    65761 gaagtttgtt ttcatttcgc aagacagtta aacgctgttc taattccgct ttgatattcg
    65821 ctacggcatg ctccatatgt tcatcattcg tcacaaagtg agtcgccggg aagattgcca
    65881 cgtgttccgt ttctcctaaa acttcacctg tcagtgcatt gacttctcgg atacgttcga
    65941 cctcatctcc aaaaaactcc acacgtaagg cacgttcatc tcttgaagcc ggaaaaatct
    66001 ccacgacatc accgcgcact cgaaaacgac cgcgctgaaa atctatatca tttctttcga
    66061 attggatact gactaagtca cgaatcaact gattgcgatc tagttctgct ccctgtcgaa
    66121 tcgaaactac ttgtttttgg tactcaaaag gcgaacctaa tccaaaaata catgaaacgg
    66181 atgcaatgac gatcacatca tttctttcta ataatgaact agtagccgaa tgtcgtaatt
    66241 tgtcgatctc atcatttaca cttgaatctt tttcgatata cgtatcgctt gaaggcacat
    66301 aagcttcagg ctgataataa tcataataag aaacaaaata ttcaacagca ttgttaggga
    66361 aaaattcctt gaattccccg tacagctgac cagccaatgt tttgttatgg gcaataatta
    66421 gtgttggttt attcactttc tcaataagat tggaaatcgt atatgttttc cctgttccag
    66481 tcgcacctaa taagatttgt gcttttttcc cgccgactac gccatccact aattggttaa
    66541 tcgcttcagg ctgatcacca gccggttggt atttcgagac aagctcaaag tgtcgggagg
    66601 tatctctctc aatcattctt cgtcctcctt ttcattcatt ccttttcatt ttacgaccaa
    66661 taaaaagaaa aggaaagcaa aggcattcct tactaaaaaa ttataccaca ccgaacatac
    66721 tttcgccact tttttatctt attatgcatg ttacgtttta taacataaaa tgttagaaac
    66781 tcgcattttt ttcatttttt cgagcttaca gtcttgtctg attcagcact tttcgcctat
    66841 aataataaaa tattggaaat ttaggaggtt gattatgaaa agaaagcatt tatttcactc
    66901 atttgttttc ttaatgacag gacttgtgac acttgcgttt ggacatgttg cacaagctga
    66961 caacactaca acggcagagt caagcacaag cgagacaatg caaaaaatag agcccaaaaa
    67021 agacgtatac gtaattgcca gtgactctgc ttttgctcca tttgaatttc aaaacgcaaa
    67081 gggcgagtac gaaggcatcg atgtaaaatt gatggaaaac atcgccaaac tgcaaggatt
    67141 taacatccga atggactacc gtggtttctc tgctgctttg caagctttgg aatcaggaca
    67201 agcagacgga atgattgccg gaatgactgt aacagatgaa cgaaagaaaa gctatgattt
    67261 ttctgattct tactttgata gcgggatcca attagctgtc aaaaaaggag atacttcgat
    67321 caagtcttat gaagatttga aaggaaaaac agtcggagca aaagttggta ccgaaagtgc
    67381 tgaattttta agcgaccatg aatctgaata tggctattct atcaaatatt ttgatgctgc
    67441 tgatcaattg tacgacgggt taaaagttgg cgcaatcgat gcaatgatgg atgattatcc
    67501 ggtaatcggt tatgcgatca aacaaggaca agaattggaa acaccgatca aaaaagaatc
    67561 tggcggaaaa tatgcatttg ccgttaaaaa aggacaaaat cctgagcttc ttaatatgtt
    67621 caatgaagga ttgaaagaat taaaaagaac aggcgaatac gataagatta ttaaagaata
    67681 tgtagcggat ggttcagaaa cttccagtga gtcttccact gctgatgaat caacatttct
    67741 tggacttatc caaaacaact atcaagcatt gttgaaagga ctttggagaa caattctcct
    67801 aactttgatc tcctttgctt tggctatagt aatcggtgtg atttttggat tattcagtgt
    67861 agcaccaata cgcgctcttc gcacaattgc tagtatttat gtcgatatca tccgaggaat
    67921 tccaatgatg gttcttgcgt tctttatttt ctttgggttg cctggtatcg ttggatttac
    67981 gattcctgac ttccttgctg gtgttatcac cttgactctt aacgcaagtg cttatatcgc
    68041 agagattgtt cgaggcggga tcaacgccgt tcctgtcgga caaatggaag cttccagaag
    68101 tttaggtctt tcttataatc ggacaatgca aaaaatcatt ttgccgcaag cgatacgcat
    68161 catgatccca tcattcgtca atcagttcgt tatctcatta aaagacacaa cgatcatttc
    68221 cgcaatcggt gtagtcgaat tgctgcaaac tggtaagatc attgttgcac gtacaacaca
    68281 aagtacgtat gtttacttga tcattgcgat catgtacttg attttgatca cagcattgac
    68341 aaaattagca aaagttttag aaaagaaggt gaagtaagat ggcagaaaaa atcttagttg
    68401 agcatttagt caaaaaatac ggcgataaca cagtattaaa cgacatcaat gtgtccatca
    68461 acgaaggaga tgtcgtctgt atcattggcc cttcaggttc aggaaaaagt acatttttgc
    68521 gttgcttgaa tcgtttagaa gaaccttctg acggtgaaat tattattgac ggcgcccact
    68581 tgatggataa aagcacagat atcaacttgg tgagacaaca tatcggaatg gtattccagc
    68641 actttaacct attccctcat ctttctattt tggaaaacat cattttagca ccgatggacc
    68701 tgaagaaaga atctagagaa gaagcagaga aaaaagcaat caaattgctg cagacagttg
    68761 gtctagaaga taaaaaagat atgtatcctg aaatgctttc cggtggacaa aagcagcgtg
    68821 tggctattgc tcgtgctcta gcgatgaacc ctgacatcat gctttttgat gagcctacat
    68881 ctgcactcga ccccgaaatg gtcggtgatg tcttgaacgt catgaaaaag ttagcagaac
    68941 aaggtatgac gatggttatc gttacgcacg aaatgggctt cgctaaagaa gtggccaatc
    69001 gtgtcatgtt cattgacgac ggaaactttt tagaagacgg aacgcctcaa caaatctttg
    69061 aaaatccgca aaatgaacgg acaaaagatt tcttgaataa agtattaaat atttaataaa
    69121 aaccaaaaat gcccggtcgc ttcatgacga ccgggcattt ttagtttctt gttttttcac
    69181 ttaactgtac cagccgcatc gcttgttctc ctgcacaaac aagcaatcgc tctacctgtt
    69241 ccatcgcata agaaagtacc atcggttcat tgacgatatc aagtacatag tcgataccac
    69301 cttcagaaac tatgatgatt atcttattct aaagctggct tttttgttgg tacaaacatg
    69361 acaactacaa aaaaaccagg cgaacgtatg attgttcgtc tggtttttaa ttattaatga
    69421 cttagcgttt ttactagaaa tagaaaattt gggcaagtct actttatatt tttactagaa
    69481 aaactacttc cgctttttcc cgccaaaagc atctttaaac ttatcgaaga aaccgtcttc
    69541 ttgctgttct tcaactttga tgccgccagc ttcagcgaat gcacgtaatg cttctctttg
    69601 ttgttcattt agatttttcg gtgtgatcag ttttactttg acatgttggt cgcctgttgc
    69661 accaccacgt aacttcggag cgccttttcc acgtaaacgg aagttcgtac ctgtctgtgt
    69721 tccagcaggg atcttcaatt tcacatctcc atggacagtt ggtacttgga cttcatcccc
    69781 caaagctgct tgtacaaaac ttaaaggcaa ttcatagtaa atttcagaac cttcacgatc
    69841 aaagatatca ctttcttcta cacgaaaaac cacatacaaa tcaccaaatg gtccgccgtt
    69901 ttctccagct tctccttgac cagctaaacg catttgttgt ccttcttcta caccagctgg
    69961 tacattcact ttaactgaat gtgctttctt ctcatgtcct gagccatgac aggttggaca
    70021 aggttctttg atttctttcc ctgtgccatg acaaacatca caagtttgac ggctcatcac
    70081 tcgtccaagt ggcgtttggc gttctacatt gatcgtacct gcaccatgac atttatgaca
    70141 agtagttggt tgtgtgcctg gttttgcacc gtttccacca caagtatgac agatttcttc
    70201 ccgattatac ttgatttctt tttctacacc aaaaatcgct tcttcaaaag acaagtcgac
    70261 cgtatattgc aaatcagcac cttgacgagg cgcatttgga tcgactgacc gaccgcctcc
    70321 tccaaagaaa gagtcgaaga tatcttcaaa accaccgaag ccgccaccag agaagccgcc
    70381 gaatccacca aatccgccac cagctccacc agcaccgtaa tttggatctg tgcctgcatg
    70441 cccgtattgg tcgtatgcag cgcgtttttg cgggtcactt aaaatctcat atgcttctga
    70501 tacttctttg aatttttctt ccgcatcagg ttctttattg atgtcaggat ggtatttttt
    70561 cgaaagctta cgataagctt tctttatttc atcttctgat gctccttttg agagccctaa
    70621 gacttcataa taatcacgtt ttgttgccat aggctttcct cctattggtt ctcatcattt
    70681 tcacgtcgct tgttatcata tcatattttg tttgaaacaa aaaccgatac ttcaaactat
    70741 ttttcaaaag aaaaagccaa agtagcaact ttggccttct ctcgaaattc agtgaactga
    70801 ttatttatcg tcgccatcga cttcttcaaa atcagcatca acgacatcat ctgctccgcc
    70861 ttgagctgct tctggatttt cttgagcttg ttgttgtgca gcttgttcgt atagtttaac
    70921 agtcaagttt tgaacgattt cgttcaatgc atcgcgttta gttttcattt cctcgatgtt
    70981 gtttgcttca acagcggctt ttaattcgtc acgagctgtt tctgcttttt tcacttcttc
    71041 ttcgtccact ttgccttcta attctttcaa tgttttgtcc acagagaata gcaatgcgtc
    71101 tacatcatta cgtaggtcaa cttcctcttt acgagcttta tctgcttcag cgtttgcttc
    71161 tgcatctttc accatacgtt cgatttcttc gtctgttaga cctgaagaag atttgatcgt
    71221 gattttttgt tctttttgtg tacctagatc tttcgcagat acatttacaa tcccgttttt
    71281 atcaatatcg aatgttactt cgatttgtgg aataccacga ggagctgctg gaatatctgt
    71341 caattggaat cttcctagag ttttgttgtc agcagccatt gggcgttcac cttgtaatac
    71401 atggatgtct actgctggtt gattatctgc cgcagttgag aatacttgtg atttacttgt
    71461 tggaatcgtt gtattgcggt caatcaattt agtgaacact ccacccattg tttcgatacc
    71521 taatgacaat ggtgtaacgt caagtaaaac aacatctttc acatcaccag tgatcacgcc
    71581 accttggata gcagcaccca tagcaactac ttcatcaggg ttaactgatt tgtttggttc
    71641 ttttcctgtt tctttacgta ctgcttctac aactgcaggg atacgagtag atccaccgac
    71701 taaaataact tcgtcgattt ctgattgtga cagacctgca tcttttaacg cttgacgtac
    71761 tggtactttt gtacgttcta ctaagtcagc tgttaattca tcaaattttg cacgtgtcaa
    71821 tgtcatttcc aaatgcaatg gtccagattc tcctgctgtg ataaatggca agctgatttg
    71881 tgtgcttgat acgccagata aatctttttt cgctttttca gcagcgtctt tcaagcgttg
    71941 taaagccatt ttgtcttgag acaagtcaat gctgttttct tttttgaatt ctgctaccat
    72001 gtagtcgatg attttgttat caaagtcgtc cccacctaga tggttgtcgc cagctgttga
    72061 caatacatca aagacaccgt ctcctagttc aaggatagat acgtcaaaag taccgccacc
    72121 aaggtcgaat actaagattt tttcgtcgcg atctgtttta tctaaaccat aggcaagagc
    72181 agctgcagtt ggttcattga cgatacgttc tacttctaga ccagcgatct taccagcatc
    72241 ttttgttgct tgacgttgtg catcattaaa gtaagctggc acagtgataa ctgctttttc
    72301 gactttttca cctaaatagt cttctgcgaa tcctttgata tattgaagga tcattgctga
    72361 gatttcttgt ggtgtatatg atttaccttc tacatctact ttgtaacctg cttcaccgat
    72421 atggcgtttg attgatgaaa tcgtatttgg gtttgtcaca gcttgacgtt ttgcaacttc
    72481 ccctacttgg atttctccat ttttgaatga tacaacagaa ggtgttgtac ggtttccttc
    72541 tgggttagcg atgatttttg cttcgtttcc ttccaatact gctactgcag agttagtcgt
    72601 acctaagtca ataccgataa ttttactcat aattaacgtc tccttcttta attgaaagtg
    72661 taagttttat atccgcacaa ttattgtgca acaatgacca tagttggtct taacacgcga
    72721 tcatgtaatt tgtagccttc ttgtagtact tctacaatcg tatcggctgg tgtatcttca
    72781 gtcgctggaa cagtttgtac tgcttgatga agattaggat caaaggcttc tcctttagca
    72841 gggatttttt caattccttc ttcttccagt gcgttacgca aactttccaa gaccatttcc
    72901 acgccttttt tcaaacctgc cccttgatca tctgacactt ctgtggctaa tgcacgttcc
    72961 aggttgtcaa ttgatggaag taattttttg gctaaatctt gtgaacggta tttttgtaat
    73021 tgctcacgct cattttttcc tcgatttgcc atatttgcga tttcagcacg agcccgcaag
    73081 tatttatctt ccatttcttc taactcagct tttaatttcg ctgtttcgaa ttcttccgct
    73141 tcagcttctg aaatacccga atcttctgca gcttcgacat cgatttccgg ttctggctga
    73201 gcatctgtca tttctttatc tagttcttct tgattttctt tcacgataca gtcttccttt
    73261 cattattcag tcagctgttt gattgctctt actaagaaag agagaacgat cagctgcaaa
    73321 tagcttcgtt attcacatta cgagtttgac aagtcaagcg agcggtagta atcagcaagt
    73381 ttagctgcta gttctcttcg ataaacatcg actagaccga acatccgaga ataaggcata
    73441 ctagtaggcc ctaacaaggc tattgtacct cttccatgac catctatctc gtaacttgct
    73501 tgaatcatac tcatattctg caataaatca ttgcctaatt cagcaccaat cttgatatga
    73561 atattgctat ccatcggagt gatcatttgt gtaagtccgt ctgagtcttt cataaaagaa
    73621 aacatcgatt tgaactgatc aagatcttga cggggctcat aatccaagag attcatttgt
    73681 ccactgacat aaaccttttc ttcaaaaaca tgaccaagaa cggtgtcaaa taactgagac
    73741 atcccttctg tcgtctggaa atatttatga agaatcatcg gaatctccgt acgaagttta
    73801 tggtacaccg tcatcagagg atctccgact agacggtcgt ttacaatctg taccattttt
    73861 tctagttcct gacttcccat atattcggga agagtgaaca cttggctttc tacattcccc
    73921 atatctgtta cgacgatagc aataatctgt cgattattca agggtacgat acgaaagcca
    73981 gtcagtcggc ggtcttttat ttcaggtccc aatgaaaaag tcgtatagct tgtcagctca
    74041 gataatatct cagcagattg cttaataatc tcattgattt catgaaattc tctgcctaga
    74101 gactgacgaa tcagcagcaa atcatcttct gctacgcgag caggttttaa caaatgatcc
    74161 acgtaataac gatagcctaa agcagatgga atccgcccag aagaagagtg agtcttcaac
    74221 agcaatcctt catcttctaa tgctttcata tcattgcgaa tcgttgctga gctggccttg
    74281 actccctcag acattaatgt tttggaacca acgggcacgc cactactcgt atagtgttgg
    74341 atgatcagac gtaaaatgtc gttttgtcgc tttgttaaca tcttcctcac ctctcattag
    74401 cactcatata ttctaagtgc taattcactt ataaatatag caagaagccc ttctcctgtc
    74461 aagagaaaga ctccattttt tagcactcta aacacaagag tgctaattct aaataattga
    74521 tgtaaatcca gttatatcaa tacttcaata cctcattgtc aaactttcta aactttttct
    74581 tttcaagttt cgataaaaaa caaatattct tcaaactatt tttcaaataa aaagcgctca
    74641 aacacattgt tccccaaaaa agtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    74701 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    74761 ttttgatagc gatcatagaa atgtttttta gtaatccctg ttttttttct tagtcctaag
    74821 aacatttctt cttctatttg attttttcgt gtcaattttt cttcttccaa taccgggcgt
    74881 tcatttgctc gtaatgggcg taaatagtgt tggattggtc ctttgttgcg gtaacgaata
    74941 tctccaagat aaccgctagc accagctcca aacccaaaat agtggtcatt gttccagtac
    75001 atgaggttat gttgagattc atgtccggag agcccaaagt tacttatttc atattggtga
    75061 cgtccatgct tttccattgc ttcaatcgtc tcttcaaaca ttctggtttc cgtttcctga
    75121 tcaggcagtt cgagtcttcc ttgtcgtacc cagttcatga acattgtttt gttttcgagg
    75181 atcaatgagt acaaagaata gtgaggcaga tccaaagcca aagctttttc caaagtatcc
    75241 cgatatcctt ctagtgtttg tcccggtaat gcataaatca aatcgatgct tacattggta
    75301 aaattttctg cttctaaaaa ttgcattgtt tgatacacat cttctgcagt atgtttacgt
    75361 ccaatttttt tcaataagcg attgtcaaat gtctgcactc ccatggataa acgattgacg
    75421 ccatagtttt tcatcacttg aagtttttca cgggtcaaat caccaggatt cgcttctaca
    75481 gtaaactcat taccattttg aaaaggcagg atctctctag cacctgataa tagtcgatca
    75541 agttgtttag cagataaaga agttggtgtt ccgcctccta cataaagtgt ttccaaattc
    75601 gctgtaggat gactttccag cattaatctc atttcattca gcagcatttc cacatactca
    75661 tctactggct gcccttctaa aaaaacttta ttaaagtcac agtaatagca gatatgctcg
    75721 caaaatggga tatgaatgta cgcagaaacc ttgttttctg cgggatttat tgtttctatc
    75781 attgaaattc tttctcctta cgtcgaaaag tcgtgcctgt tttcgacctt cttaaaatcg
    75841 cgttcaagca ccaactcatt gaaataagcc gcatatttag aatcaaggaa acctagctct
    75901 aaaaatgctt gcttattgtc atcggagcta cacggtgctt ttacagcctc tcgataaacg
    75961 gtaatcgcaa agctgacctt cggcattaag tcaaaatctg caaaaatatg aaaagctatt
    76021 ttcacagatt cctccttaag tgctcagacc acaacgcttt gcttacaacc tctcatatac
    76081 gcatactaaa cgtatttggc gaacaaatcc actaaaaaat ccaagaatcg acttcttgga
    76141 tgattttttt acactcatga ttcttcctaa acaattttgt ccttatttta ttcataaaaa
    76201 aatctagtca aatctctcac ttcaagagtt ttaactagat tccaattatt atttttttgt
    76261 aaaaataaac agtctcttaa tcatagacat ttcttcggtg ccctacttga atagcgataa
    76321 caacgaattg attatcttct atctctacca atacacgtat ttgccaatca gagctgcttc
    76381 tgaatttcgg gcgtgaattt taaacggcag gtcattccag cccaccaaat tcattaagga
    76441 ttcttccatg aatactgttg ttttcccact tcttatccaa tgtttatggg caatttcagc
    76501 cgtttgacga ttgtattcat cttctagttg ttccatcgaa tagtttttta gaagttcaga
    76561 taaagtaatc ccataaaatt cagccatata agaaagccat tcttttttgg cgtcacttac
    76621 acgaaccgta atcatagccc tataattgcc tctcttaaag aatctcatgc atttcctgat
    76681 gtgattttag aaaaatttac ttgctaaaaa actgtgaaaa tgtaggagct agcatccttg
    76741 tagtaaatga tcgagctctt tattatacat taaaaaagtt cagaaatttt gatgaagtag
    76801 ccaactactt tttcatgagt aaagcaacac tagaaaaaga atcctccagt acttagaatt
    76861 tactggagga tgtacttttg aagaagccta taggttattt aaaaaatatt actacaggaa
    76921 ggaacgagaa ttacttcgtt acttataata gaacttagtc ttatatagaa ggcatagctc
    76981 acattagctt atttgtccta taaaaataaa ttacggtatt ttagaaaagc tattttctct
    77041 tttttcaacg atcttcagca atttttcttt atcgtcaggt aactcaagtt catgaattct
    77101 agcatgacaa ttgggacata acgcgataca gttttcaatc acatcttttc caccttgcga
    77161 caaatattct atatgatgac aatgtaaata aggtacaccg tcttttataa atggcccttt
    77221 ttcttggcaa agttgacaaa taccttttgc aagtcgttta acatattcag atacgaaggg
    77281 attacgttta tatatttttg tttttgagaa gcaaaactcc tcttcgttaa catattttgc
    77341 agccattttt ttattataat tactaacttt tcttgcttct tcctctaatt tagcattaga
    77401 taaattacga atatgtc
//
LOCUS       DADXTG010000002        58745 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.002, whole genome shotgun sequence.
ACCESSION   DADXTG010000002 DADXTG010000000
VERSION     DADXTG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.002"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..206)
                     /locus_tag="IWI57_000064"
     CDS             complement(<1..206)
                     /locus_tag="IWI57_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448240.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLS"
     gene            complement(381..2516)
                     /locus_tag="IWI57_000065"
     CDS             complement(381..2516)
                     /locus_tag="IWI57_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2448241.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2599..4254)
                     /locus_tag="IWI57_000066"
     CDS             complement(2599..4254)
                     /locus_tag="IWI57_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBC2448242.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4256..4570)
                     /locus_tag="IWI57_000067"
     CDS             complement(4256..4570)
                     /locus_tag="IWI57_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBC2448243.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4740..5000
                     /locus_tag="IWI57_000068"
     CDS             4740..5000
                     /locus_tag="IWI57_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBC2448244.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5014..5328
                     /locus_tag="IWI57_000069"
     CDS             5014..5328
                     /locus_tag="IWI57_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBC2448245.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(6058..6612)
                     /locus_tag="IWI57_000070"
     CDS             complement(6058..6612)
                     /locus_tag="IWI57_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBC2448246.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6738..8666)
                     /locus_tag="IWI57_000071"
     CDS             complement(6738..8666)
                     /locus_tag="IWI57_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBC2448247.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8972..9688)
                     /locus_tag="IWI57_000072"
     CDS             complement(8972..9688)
                     /locus_tag="IWI57_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBC2448248.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9689..11512)
                     /gene="glpO"
                     /locus_tag="IWI57_000073"
     CDS             complement(9689..11512)
                     /gene="glpO"
                     /locus_tag="IWI57_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBC2448249.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11526..13022)
                     /gene="glpK"
                     /locus_tag="IWI57_000074"
     CDS             complement(11526..13022)
                     /gene="glpK"
                     /locus_tag="IWI57_000074"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBC2448250.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13158..14618)
                     /locus_tag="IWI57_000075"
     CDS             complement(13158..14618)
                     /locus_tag="IWI57_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2448251.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14608..15903)
                     /locus_tag="IWI57_000076"
     CDS             complement(14608..15903)
                     /locus_tag="IWI57_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBC2448252.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16235..16912)
                     /locus_tag="IWI57_000077"
     CDS             complement(16235..16912)
                     /locus_tag="IWI57_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBC2448253.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16942..18033)
                     /locus_tag="IWI57_000078"
     CDS             complement(16942..18033)
                     /locus_tag="IWI57_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBC2448254.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18134..18568)
                     /locus_tag="IWI57_000079"
     CDS             complement(18134..18568)
                     /locus_tag="IWI57_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2448255.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19152..20609)
                     /gene="ascB"
                     /locus_tag="IWI57_000080"
     CDS             complement(19152..20609)
                     /gene="ascB"
                     /locus_tag="IWI57_000080"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBC2448256.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20606..22501)
                     /locus_tag="IWI57_000081"
     CDS             complement(20606..22501)
                     /locus_tag="IWI57_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2448257.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22559..24334)
                     /locus_tag="IWI57_000082"
     CDS             complement(22559..24334)
                     /locus_tag="IWI57_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBC2448258.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24384..25880)
                     /locus_tag="IWI57_000083"
     CDS             complement(24384..25880)
                     /locus_tag="IWI57_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2448259.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26074..27696)
                     /locus_tag="IWI57_000084"
     CDS             complement(26074..27696)
                     /locus_tag="IWI57_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBC2448260.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28145..29458)
                     /locus_tag="IWI57_000085"
     CDS             complement(28145..29458)
                     /locus_tag="IWI57_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBC2448261.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29703..29927)
                     /locus_tag="IWI57_000086"
     CDS             complement(29703..29927)
                     /locus_tag="IWI57_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448262.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29947..30369)
                     /locus_tag="IWI57_000087"
     CDS             complement(29947..30369)
                     /locus_tag="IWI57_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBC2448263.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30512..31222)
                     /locus_tag="IWI57_000088"
     CDS             complement(30512..31222)
                     /locus_tag="IWI57_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448264.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31240..31737)
                     /locus_tag="IWI57_000089"
     CDS             complement(31240..31737)
                     /locus_tag="IWI57_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2448265.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31774..32226)
                     /locus_tag="IWI57_000090"
     CDS             complement(31774..32226)
                     /locus_tag="IWI57_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2448266.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32361..32966)
                     /locus_tag="IWI57_000091"
     CDS             complement(32361..32966)
                     /locus_tag="IWI57_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBC2448267.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33416..34036)
                     /locus_tag="IWI57_000092"
     CDS             complement(33416..34036)
                     /locus_tag="IWI57_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2448268.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(34038..34907)
                     /locus_tag="IWI57_000093"
     CDS             complement(34038..34907)
                     /locus_tag="IWI57_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBC2448269.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34930..35337)
                     /locus_tag="IWI57_000094"
     CDS             complement(34930..35337)
                     /locus_tag="IWI57_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBC2448270.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35356..36645)
                     /locus_tag="IWI57_000095"
     CDS             complement(35356..36645)
                     /locus_tag="IWI57_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2448271.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36672..36974)
                     /locus_tag="IWI57_000096"
     CDS             complement(36672..36974)
                     /locus_tag="IWI57_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2448272.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36999..39092)
                     /locus_tag="IWI57_000097"
     CDS             complement(36999..39092)
                     /locus_tag="IWI57_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBC2448273.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(39581..40345)
                     /locus_tag="IWI57_000098"
     CDS             complement(39581..40345)
                     /locus_tag="IWI57_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBC2448274.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40456..41325)
                     /gene="mdcB"
                     /locus_tag="IWI57_000099"
     CDS             complement(40456..41325)
                     /gene="mdcB"
                     /locus_tag="IWI57_000099"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBC2448275.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(41350..42426)
                     /locus_tag="IWI57_000100"
     CDS             complement(41350..42426)
                     /locus_tag="IWI57_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBC2448276.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(42450..43043)
                     /locus_tag="IWI57_000101"
     CDS             complement(42450..43043)
                     /locus_tag="IWI57_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBC2448277.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(43047..44723)
                     /gene="mdcD"
                     /locus_tag="IWI57_000102"
     CDS             complement(43047..44723)
                     /gene="mdcD"
                     /locus_tag="IWI57_000102"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBC2448278.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVSLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(44713..45012)
                     /locus_tag="IWI57_000103"
     CDS             complement(44713..45012)
                     /locus_tag="IWI57_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBC2448279.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(45114..46760)
                     /gene="mdcA"
                     /locus_tag="IWI57_000104"
     CDS             complement(45114..46760)
                     /gene="mdcA"
                     /locus_tag="IWI57_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBC2448280.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(46767..47678)
                     /locus_tag="IWI57_000105"
     CDS             complement(46767..47678)
                     /locus_tag="IWI57_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBC2448281.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            47798..48655
                     /locus_tag="IWI57_000106"
     CDS             47798..48655
                     /locus_tag="IWI57_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2448282.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(48842..49204)
                     /locus_tag="IWI57_000107"
     CDS             complement(48842..49204)
                     /locus_tag="IWI57_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBC2448283.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            49550..50989
                     /locus_tag="IWI57_000108"
     CDS             49550..50989
                     /locus_tag="IWI57_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2448284.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(50996..53548)
                     /locus_tag="IWI57_000109"
     CDS             complement(50996..53548)
                     /locus_tag="IWI57_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBC2448285.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(53662..54993)
                     /locus_tag="IWI57_000110"
     CDS             complement(53662..54993)
                     /locus_tag="IWI57_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2448286.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(54959..56437)
                     /locus_tag="IWI57_000111"
     CDS             complement(54959..56437)
                     /locus_tag="IWI57_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2448287.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(56742..58577)
                     /gene="tdc"
                     /locus_tag="IWI57_000112"
     CDS             complement(56742..58577)
                     /gene="tdc"
                     /locus_tag="IWI57_000112"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBC2448288.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        16845 a        12570 c         9900 g        19430 t
ORIGIN      
        1 tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa
       61 aagacccata taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc
      121 caaatcttta accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca
      181 acttcttctc ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg
      241 agtttaccat acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa
      301 agaacaaaac atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct
      361 ttttattttt tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct
      421 ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat
      481 ggatataaga ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg
      541 cagttttcta atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct
      601 tctgtttggg tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt
      661 attttcaaca attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt
      721 cggatcgata cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg
      781 attgctccgc tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag
      841 tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc
      901 tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt
      961 gccagggtat aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc
     1021 ttttcaacaa gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg
     1081 ggttgccaag cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc
     1141 tcatccaccc atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa
     1201 agtttccttg cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat
     1261 aattccaact gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt
     1321 gcttgtttca aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc
     1381 ctatcgatca atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta
     1441 gaaaggactg tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac
     1501 aaaggatctg attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc
     1561 tccgatacat tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct
     1621 gctgaaagct gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat
     1681 gatggtagga cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag
     1741 tcatcggatg tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata
     1801 gcagaggttt taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc
     1861 cgaatgattg tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc
     1921 tcatcttggc ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa
     1981 aacttgacca aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg
     2041 attttatttt gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg
     2101 gcaattggtg aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct
     2161 gtaccgatat aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca
     2221 aagtagggac ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt
     2281 ttgatattca tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg
     2341 ttatctaagt aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg
     2401 tttccttcta tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc
     2461 ttttgataca cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac
     2521 attctcctct ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg
     2581 caagcttttt aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg
     2641 atataaagaa aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct
     2701 ttccgctaga tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc
     2761 taattcattt aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt
     2821 gcccttttcc aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc
     2881 tgtcggcaat tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc
     2941 atatcctagc atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc
     3001 taattccggt aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat
     3061 ccgtttatcc acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg
     3121 atggaagatc aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt
     3181 tcctgataca tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa
     3241 accggtggat gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg
     3301 atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg
     3361 tgaagcaaga gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc
     3421 ccccaccgca taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc
     3481 gcgtatgcct aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat
     3541 ggataaaata actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc
     3601 gtggtcttca aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa
     3661 ttctttttct ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg
     3721 ttcgatcacc atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat
     3781 gaatcctgca ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt
     3841 atcgatatct ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat
     3901 cgatggaaca actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg
     3961 tttttgaccc tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc
     4021 atgatttgga cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag
     4081 aagattttcc cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa
     4141 agaaacatac ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag
     4201 ccgtgtagca gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt
     4261 tccctcctac tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat
     4321 aaccagattt ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca
     4381 catagacggt cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt
     4441 tagctccttt tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg
     4501 gattttccaa aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata
     4561 gcaaattcat tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa
     4621 caggagcttt attaatatgt caaccagaat gactcttcat ttttttcact actttttata
     4681 tttgcataaa ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta
     4741 tggagattga taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg
     4801 gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg
     4861 ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt
     4921 gctttgagaa acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca
     4981 agatgattat taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa
     5041 ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa
     5101 acgacgattg tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat
     5161 gcagaattct tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct
     5221 aatcatattt cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt
     5281 gatttagttg aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt
     5341 gcgacagaag tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg
     5401 aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa
     5461 acaaaaatag cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
     5521 tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa
     5581 aaacgggaaa atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg
     5641 attgaacacc gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa
     5701 aattttgtga aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg
     5761 gcttttgaat accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga
     5821 gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc
     5881 agctgattga acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa
     5941 gaaataattt caaaaacaac ttctcctgtt tttggtttgg ttggcttatt atcgagaagt
     6001 agctactggg agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta
     6061 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
     6121 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
     6181 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
     6241 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
     6301 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
     6361 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
     6421 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
     6481 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
     6541 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
     6601 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
     6661 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
     6721 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
     6781 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
     6841 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
     6901 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
     6961 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
     7021 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     7081 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     7141 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     7201 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     7261 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     7321 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     7381 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     7441 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     7501 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     7561 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     7621 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     7681 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     7741 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     7801 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     7861 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     7921 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     7981 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     8041 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     8101 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     8161 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     8221 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     8281 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     8341 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     8401 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     8461 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     8521 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     8581 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     8641 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     8701 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     8761 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     8821 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     8881 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     8941 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     9001 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     9061 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     9121 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     9181 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     9241 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     9301 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     9361 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga
     9421 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     9481 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     9541 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     9601 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactctc
     9661 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     9721 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     9781 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     9841 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     9901 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     9961 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
    10021 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
    10081 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
    10141 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
    10201 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
    10261 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
    10321 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
    10381 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
    10441 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
    10501 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
    10561 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
    10621 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
    10681 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
    10741 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
    10801 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
    10861 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
    10921 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
    10981 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
    11041 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
    11101 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
    11161 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
    11221 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
    11281 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
    11341 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
    11401 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
    11461 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
    11521 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
    11581 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
    11641 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
    11701 gccgttgttt ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
    11761 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
    11821 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
    11881 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
    11941 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
    12001 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
    12061 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
    12121 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
    12181 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
    12241 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
    12301 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
    12361 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
    12421 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
    12481 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
    12541 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
    12601 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
    12661 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
    12721 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
    12781 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
    12841 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
    12901 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
    12961 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
    13021 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
    13081 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
    13141 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
    13201 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
    13261 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
    13321 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
    13381 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
    13441 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
    13501 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
    13561 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
    13621 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
    13681 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
    13741 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
    13801 tctactatcg aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt
    13861 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
    13921 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
    13981 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
    14041 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
    14101 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
    14161 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
    14221 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
    14281 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
    14341 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
    14401 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
    14461 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
    14521 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
    14581 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
    14641 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
    14701 ttcaaacgtc catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc
    14761 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
    14821 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
    14881 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
    14941 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
    15001 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
    15061 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
    15121 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
    15181 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
    15241 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
    15301 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
    15361 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
    15421 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
    15481 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
    15541 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
    15601 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
    15661 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
    15721 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
    15781 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
    15841 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
    15901 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
    15961 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
    16021 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    16081 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    16141 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    16201 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    16261 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    16321 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    16381 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    16441 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    16501 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    16561 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    16621 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    16681 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    16741 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    16801 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    16861 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    16921 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    16981 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    17041 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    17101 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    17161 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    17221 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    17281 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    17341 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    17401 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    17461 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    17521 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    17581 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    17641 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    17701 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    17761 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    17821 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    17881 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    17941 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    18001 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    18061 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    18121 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    18181 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    18241 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    18301 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    18361 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    18421 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    18481 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    18541 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    18601 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    18661 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    18721 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    18781 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    18841 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    18901 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    18961 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    19021 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    19081 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    19141 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    19201 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    19261 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    19321 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    19381 tgtttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    19441 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    19501 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    19561 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    19621 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    19681 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    19741 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    19801 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    19861 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    19921 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    19981 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    20041 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    20101 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    20161 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    20221 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    20281 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    20341 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    20401 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    20461 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    20521 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    20581 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    20641 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    20701 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    20761 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    20821 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    20881 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga
    20941 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    21001 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    21061 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    21121 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    21181 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    21241 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    21301 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    21361 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    21421 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    21481 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    21541 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    21601 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaagtaattg
    21661 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    21721 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    21781 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    21841 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    21901 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    21961 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    22021 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    22081 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    22141 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    22201 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    22261 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    22321 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    22381 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    22441 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    22501 ttcaattagt accccttata atagcgaacg aaactgtaca atagtgtgta tggtttactt
    22561 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    22621 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    22681 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    22741 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    22801 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    22861 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    22921 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    22981 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    23041 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    23101 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    23161 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    23221 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    23281 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    23341 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    23401 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    23461 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    23521 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    23581 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    23641 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    23701 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    23761 tactgatttg ttgaggcaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    23821 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    23881 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    23941 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    24001 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    24061 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    24121 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    24181 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    24241 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    24301 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    24361 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    24421 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    24481 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    24541 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    24601 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    24661 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    24721 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    24781 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    24841 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    24901 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    24961 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    25021 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    25081 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    25141 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    25201 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    25261 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    25321 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    25381 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    25441 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    25501 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    25561 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    25621 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    25681 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    25741 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    25801 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    25861 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    25921 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    25981 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    26041 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    26101 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    26161 aacccttaaa gcttcacgtg gaacagtgaa cgtctgcttt tcactggaca tattacaaat
    26221 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    26281 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    26341 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    26401 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    26461 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgcattga ttgattccaa
    26521 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    26581 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    26641 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    26701 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    26761 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    26821 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    26881 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    26941 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    27001 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    27061 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    27121 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    27181 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    27241 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    27301 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    27361 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    27421 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    27481 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    27541 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    27601 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    27661 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    27721 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    27781 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    27841 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    27901 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    27961 gaggaactat tttttcgttt ttttctctaa tactcaggcc agaacactga tttcacaacc
    28021 tctttataaa cggtgttcac tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata
    28081 taagaaacta ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac
    28141 ctctttattt cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca
    28201 tgattggaga tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa
    28261 aacctaatgc aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga
    28321 atgaagggat cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca
    28381 atactcctac cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg
    28441 gagaaaagaa caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac
    28501 gtcctccaac ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa
    28561 ataaggaacc cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca
    28621 tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat
    28681 caaagaaagt cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt
    28741 cccctacatg ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa
    28801 tttcagaagg cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgccatcc
    28861 cgatgaagat gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa
    28921 aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat
    28981 tcgaaacgat gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc
    29041 cagaaatcgc atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga
    29101 agattgtgat caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc
    29161 atggaatccc cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag
    29221 gtgccgttcc aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg
    29281 ctaatgcagt cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg
    29341 gatttacaaa taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac
    29401 gttttaccgt ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct
    29461 tccatccccc taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata
    29521 aaacaacgtg ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt
    29581 aagcaaaatc cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc
    29641 actttggatt acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa
    29701 aatcaatagt gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt
    29761 gtatcgacag gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag
    29821 ccttttgtct tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt
    29881 gctcgccgat acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct
    29941 ttttattcaa tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga
    30001 aaaattgaaa gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc
    30061 gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc
    30121 tggtgaaaac ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat
    30181 gctgagtaca atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc
    30241 aattttttct acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg
    30301 tgttttttgt atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac
    30361 tgattccatt atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata
    30421 aagcaatagt ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaaacggc
    30481 aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg
    30541 ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt
    30601 cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg
    30661 ctgcttgtgt ttcttccgtc agcattgttc tttgggcttc acgccagttg agacagcgac
    30721 agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat
    30781 ctgcacctaa aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg
    30841 cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat
    30901 agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta
    30961 aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc
    31021 actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt
    31081 ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat
    31141 cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg
    31201 tatccactat tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa
    31261 tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa
    31321 tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat
    31381 ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat
    31441 accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc
    31501 catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc
    31561 gatactcact aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag
    31621 tgcataagct tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt
    31681 atctgtaaat gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt
    31741 tcgaatttcg tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt
    31801 gacaaaattc caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg
    31861 ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg
    31921 aatgatgaaa ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt
    31981 attttcttta tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg
    32041 ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg
    32101 attttcgcaa atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa
    32161 ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc
    32221 agccatttca tcccaccttt attttgtttg taagactagt ttacctcttt ttgttgcaaa
    32281 tgcaactatt ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt
    32341 ttcttctttt acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt
    32401 aactgtttct tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat
    32461 cccatgttgg aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa
    32521 gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca
    32581 aaaactgctc gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta
    32641 cgcacatttt cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct
    32701 ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca
    32761 tctacttttg gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa
    32821 gtttcttttc ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc
    32881 tctttatcaa atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt
    32941 ttgtattgag ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta
    33001 tgtaatcaag tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat
    33061 aaataaaaga atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga
    33121 ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga
    33181 cgagctattc ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc
    33241 cccactaaac ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca
    33301 tgagaagctg tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga
    33361 cctctcggta aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact
    33421 tattgtcatt gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat
    33481 agtctgttac atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg
    33541 cgatgatctt tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca
    33601 atacgtcacc atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat
    33661 cacgtttgta ccgacgttga cataacactt caaagtctgc tgttaggcga atcgtgatca
    33721 cctgatatcc gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag
    33781 gatactcgga cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat
    33841 agaaaggata aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga
    33901 tggattctgc atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc
    33961 gttcccgtag tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa
    34021 tgaaatactt tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac
    34081 agtccaatct tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg
    34141 cgaagagact tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg
    34201 actaaaagct ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa
    34261 tcttcttctt tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg
    34321 atttctttca gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta
    34381 ccgatagcaa tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc
    34441 ggtcttgtat ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt
    34501 gttccaagtt ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt
    34561 gtcttcagga tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat
    34621 ccgctgtcga ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact
    34681 gcatcaacca tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg
    34741 atatatttag cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct
    34801 gcgcctagca tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa
    34861 ttcaactctt ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac
    34921 cttatctttt tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc
    34981 cattttgccc atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg
    35041 cttctggtta tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc
    35101 caaactcttt tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag
    35161 tccaacttgt tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg
    35221 tttcaattgt gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc
    35281 ttcttttgcc agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat
    35341 tttccctact ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag
    35401 tgctgcggct actgtatcta catcggttgc tgtagcattg atgaatccca tcgaattaaa
    35461 gatcgttact agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat
    35521 gatcgctcct cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa
    35581 gaaattggtt agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat
    35641 ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata
    35701 aggaaagaaa actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat
    35761 ccctcgaaat gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata
    35821 cacacccact acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc
    35881 tgataattca gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac
    35941 catcacgacc atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt
    36001 gatatcttcg atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc
    36061 ttcaaataag tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat
    36121 tttacgaatg atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa
    36181 accaccgacc aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt
    36241 catcgttgcc atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa
    36301 acgagccaaa ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc
    36361 taaacccaaa tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg
    36421 catatgaaat ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct
    36481 cgaaccggca ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg
    36541 tcctgctgat ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga
    36601 tgcctgagtc aaaatatttt cgatgataaa attgataacg cccatgtttt taccctcttt
    36661 tctttttgta tttagataac tcccttatct tgcagaactg gggtgatttt ttcttcaatt
    36721 tcattttttg aaacaatatt tttcaaatag atcatcgccg ttttaggatc aactgcgaac
    36781 ttttcgaact gcgaacgaaa attttctgct gtgatgatca catctggttt catggaaact
    36841 gcggaagaaa tatcacaatg gtccagtttg gcttctactc ctaatttgtt caaaacatcc
    36901 tcagcagaca tctgtgctgc aaaactactt cctaaacctg caccgcatac gaataaaatg
    36961 ttcaatttgt tcatctttgt tctcccccgt atcatttatt atttctcaac tactgaaaaa
    37021 agcagttgtt tgaaatcttc gatatttgtg cattcaatca atcgatccaa cttctcttcg
    37081 ttgttgatca attcaattag ttctttcatg atgttcagat gcgagtagga gtctactgct
    37141 gccaaacaaa aaataatctt tactggatcc atttcaggat taccgaaatc gattggttgc
    37201 tcaatcgtag ctacgctgac tcctaattta ttgaccccat cttctggtcg ggcatgggct
    37261 agtgccatgt gtttgccaat cacgatatac ggtccgtaat cattcactgc acgaatcatc
    37321 gcttcgacat agcgctcttc gacaatgttt tctttaacca atggtctagc agctagttca
    37381 atcgattcct cccaagtagt ggctttttct ttgatcatga tattgctgtc cgtcaaaata
    37441 tcttttaaca taggctggat ctcccttttg ttgatttcta aattgtttgt ttcgaataat
    37501 ttttctaact tgccatagat gattcgatct actcggccac cactttcttt catgacatct
    37561 attatttgag tgaataactc cgtcgattca tgactgttag gctctaaccg ctgtagttct
    37621 tcatttcttt tcaaaaagtc ttctataacc aagatattcg tctcagttaa aatcggatcg
    37681 acaactaaaa gcggcttatt tcgataattc aattgaaagg tgctgaaaac cagattgaca
    37741 tccaatttat ctactaattc gatttctttt gaacttaaaa ctgcagtaat ctcgatctga
    37801 ggaaactttt ctttcagatt ctctgctaac agatttccag tagccatccc atggttacag
    37861 acaacgactg atttataaac ataattcaag tctcgcttga ttacactggc gatcgtcgaa
    37921 aagtggatga caagaaaagc tatctcgtcc tccagtatct tgccaccaat tacttcttcg
    37981 atcgtaggaa caaaatgagt gactgcgcgg tagattggga aataatttgt tcggatgttt
    38041 tctttcaatg gattcatgac ttggatatga ttcttgattc ttacaatcag tgcagccatg
    38101 tgcttataga gattttcgaa taaagcttct tcctttagat ggaaaggtac gtgtgtctcg
    38161 ttctctacaa attggatcaa ttggatcgtt agtaattgag cttgtaccca ctcgattgaa
    38221 ttggagatat ctctagcatt gaacgtctca atagtgaaac gaagataatt tctttctact
    38281 tgcgggacat tgtccaattg aaattcctcg atcatgtggt caataaatgc agaaaagcta
    38341 ctgtctttta tttctgtatt tttccaatta atggcagaga taaattcatg tttcctgatt
    38401 cgctggatcc agatcaatgt aaaaagcagg atttgttttt tataaatatc atcttcttgt
    38461 ttcaacaact tattggaata gatctcctgt atttttttga tttccaccaa aggaaaataa
    38521 tggagaaaga ttttttgagc cggtgttaat tgtgcatctt gattcataaa gaagtcggtc
    38581 attcccagat tctgattgat aaaatcatag atcatcgtac ggatagatcg ttctgaacct
    38641 ttataaacca gtccttgctt tccataactg acgatctcta tcccataact gaccagtgtt
    38701 gctcgtaacc gtctcatatc ttcatctaat gtacttttag agatttgaaa ttcttcttct
    38761 tttttgttta gatggatcgg gattcggctg taagcaacgg ctaaaactag atcaaaaata
    38821 cgttcgcgcc gattcaaaaa gccgtctttc acatctttta tttcagtttc taatcgagct
    38881 tgcttttcct tttccagctt caacaaaaaa actttgttgc gaattgtacg gatctccgga
    38941 agctttttac tgctcaacca gatattcgct tcaagaactt cctgacggat cgtccgctga
    39001 ctgacattga actcacttgc tagatgtttt gcttttactg gatattgggc tgtcatgatt
    39061 ctgtgaagta tttcaattgt tcttgttttc atagaaatga tctccttctg atttcaagta
    39121 tatggttccg cttacataat aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt
    39181 aagcaaagca aacaaaaaat aagccatatg ctttttcatc tcctcaacga aaaagacata
    39241 cgcctattat tacaaaaatt ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc
    39301 ctgcatcaag taataagaac aactaaacta aaatagtgct tcctattttt cgtttggttg
    39361 ttcttattat cgcagatgca agcttttaaa caccatttag tggagaggtt gtgacagaga
    39421 tgttcagcct tgagcgataa gaagcgattt tgaaaaatcg cttcccatat tttacaaaat
    39481 cgtgccttat tgccgatagg ctatctctgg cacactgttt attcagaaaa aggagctgcg
    39541 acaaggcttt tgtcacaact ccgttcataa atatttcctg tcaatccagc ataatcggct
    39601 gaataaaatc ggcttgccat cttttttctt tctcagaaaa atcaaaatac catatctgag
    39661 aaaaatactg tcgttgtgtc ttacttcctg agatcaatcg cccatctttt cgatcgactg
    39721 aataatcaca gcatctgaaa tcaattcgga tggcaaaact attttcgtgg atctgtcgat
    39781 aatttcctac ttcctgaata gttatttttt tagtatgatt tcttacaccg tctgctttgt
    39841 ttttttgcag aactttctga tgttcggcaa acagcttgtc tgtataacag cttctggctt
    39901 tttcaggagt ttcttcttcc caagcggatt ggatcttcat gaatatcttt tggatttcca
    39961 gaaactgtgc tttcttttct ttagccgttc ctgacatgcg ggacaatatc tccgctttca
    40021 gccttttctt ttgtctttta ttcttgacga tagaaaaagc agaaacccct cctactgaca
    40081 aaacagctaa tgcaagaaat cccgtttgct cataactaga aggacctgta cctggaccat
    40141 aatacccata agaatctctg ccgtaagaac gactgccaaa ataactgccc ccatatgaac
    40201 gaccaccaaa tgaacctctg cccgaacttc gaccgctaga tgtacctcca ccatgaccac
    40261 ccgcgacagc gaatccttgg gtcggcagag caaacaaaca taggcccact aacaaaatga
    40321 ttgctaaata attgattttt ttcattgtct tcctcgttat cttttattac aaaaaactat
    40381 ttttatgata gccttgctaa aaggaagagt caataaccag atgcataaac tgttttttta
    40441 gcaattttgt cttatttagt cattgagtaa catcggacaa atgtatgtgt cggcagtcca
    40501 taattcctct atcttcagca tgaacaagct tgcagacaag agatctgcgc ttcctccagg
    40561 agagatttgg ttttcctgac aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc
    40621 actttgacta gcagcatgag caagtttttg catttccact aacgttttct gatcactgcg
    40681 atgcaatacg catgtgtcat ctagcgaact catcaaaact aacaacatat gcatttgttt
    40741 ctgtacatat gaatcattat ctagctcctt agctgcttgg attgcaagcc tgatatgcgg
    40801 ataccctaat atcgcttctt catatgcacc atttacgcca tatttttgct tcaccctatg
    40861 accatgagtg gtttttttat gacgatattt tgtatcagga aaagcagcaa gcttgctgac
    40921 atcatcaagt attttcgata aagaaaaagg ctgttccata ctgatttttt gtgcaatgac
    40981 actgatcaat agccccattg cccaaatcgc tcctttatgg gtattgatgc catttgtagc
    41041 aatcagcatt gccttttccg cttttcgccc aatctcagca attctttctc ttaactcctg
    41101 atcaatagca tgatgccatg ctatttgagc catttcttca aaaaaaggag ttagcgtctt
    41161 agcagactta agaaataaag catagtccat atcggaatgt gcacccgcat ccagtgcatc
    41221 cactaatcca ggttttggac ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc
    41281 agcgagttgc tgagtcactg ctttttttcg ttcgtcattt atcatgacac tcactccttc
    41341 ttcctactat tacttgctga aacctttttt gatagctacc caaatgatat gaagtaacca
    41401 gcttaaagca aatgcacaac ttgccgtcat tagataagct acaagaatca tgactcctga
    41461 aggtaactgc agccaactat ctttaaacaa taataacagt acttggaaaa caaatacatt
    41521 ggctaaatac gtacgataag cataaacaga caaccatttg aacaatggca attttggcga
    41581 atcgtttaca atcattttgg aagcgcctat gaagacagca aagatgatga ctaaactgta
    41641 taaactttgg atcgtattca agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc
    41701 gagcaatgaa cggacagaaa agaacgcgat aactaaggca acaggtaaga aaagataacg
    41761 tactctgtcc aaatattgat agactttttc gtgataaaca aatgcggcta ccccataaat
    41821 catgaaacta gcaacaaacc gatctaataa gtaccagctg tcatgataag gcccagtcaa
    41881 tacttgtgtt tgatagaaca gataccatgc gacatataaa atcgttgaac caatcaatac
    41941 tggccataca tattttttat tagcaaatac tttgtaaccg agccaaacaa aaaacggcat
    42001 gaacaattgg atctgcagca tcataacggt ataccataaa tgaggcgctc catcacctgt
    42061 gacatatttc agtaaaaagc ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg
    42121 aaatagccaa agataagccg tcgtccatag aagatacgga acgactagtt caaaaaactt
    42181 ttctttcaaa aattcccaat aagacaaatg ctgacttgtt tttaccatat tataaacaat
    42241 tgcaaaaata aacattggtg ctgtgtattt aacgatgacg taaaaggtac caagaaaagc
    42301 tgtatttgtt gcgctgtggc tgtttgccaa agcaaagctc aatatcgttt gcatcataac
    42361 agccgttaca gctgatactt tctggaagtc acttacgttg attttgattt ttttgttttc
    42421 aaacatctct ttctctctct ttttctttat taccaaagtt ctttggttaa tttaggtcct
    42481 tcgactgttt tcaatagaat ttttttctct ggctctctca aatactcttt tagtgaaaaa
    42541 ccgccttgcc gagtcactac ttgggtatcg aattgctcaa aaaaagcagg atgcgattga
    42601 attacctgaa ttggcaattc aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg
    42661 gtttctttta ctgccggaat cccagtcgat aattcaaatc ctaagctgcc gccgattccc
    42721 catttacatc ctgataatat gtttctagca gcatgaaatt gctgataaac aggataatcc
    42781 atgattttct cttgtctata cgattcttgt tcgatgatgt cccacggatg aatgacttgt
    42841 ttgacggcat gttttgagat ttctgttgac catctctgat tgcgggcagg tccacgtaaa
    42901 ccaatcggta gtatttctgc gtgatccgtc gacgcacgac gaactgttcc gaacatcttt
    42961 tcagttagaa cgacccattc aggcaaggtg aacccttcta tttcatgttt atcaaaaagg
    43021 acaatatcat gtgcttgaat cattttttat gcatcccatt cttcgtccat tttggcacgg
    43081 actttatttg tttcgacacg tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc
    43141 agcatcgtgt catttgtatt gcggacatct tgaatggctt cattgattgc ttcgatgact
    43201 ttgctgacat tttgttctga agcagcttgg tcggtgatat cttctaggaa atgatagatc
    43261 gctcctagtt ttgtataatt attgatatcg taagcgatag ctggaacttt ttcagcagct
    43321 gcttcgattt cagcaaccga acgttttgtc acacgtgcag cactttcttt agacatcgct
    43381 tggacggtaa tttctttatt gtttaaagca atcaagcggc ttgattgtaa tccatgtgct
    43441 aaaaatgcac ctgaaatagc atttcctgca atcaatccaa taactggatg tccagcttga
    43501 cggagttttg cataagcagc agcactatta gccaaagcca catggatacc gatcaattct
    43561 tcgttatagc cgtatgcttg gcttggtaca tcgatcacaa cgatgattgg ccgtttcact
    43621 tttttgtctt tatcttcttc gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca
    43681 actaaactaa cttcaccgtt tcgtacacgg tacatcgggt ttttaggatc aggtacgatt
    43741 gatgtcacga tggcttcttc tatgaattta cttgatttac cataatacac agtactaata
    43801 gaagaagttg gatttttcat gccagttagt gccgcaaacc aatcgtaacc aacacttgct
    43861 gcagctggtt catttccttc tgctacttct ggtaagtcaa ttgattttgc ttgatggtta
    43921 gcaattgctt cattgtaatc gtcaatggcc attggttttg aaagatctaa agaatcaagt
    43981 aatgataaat aaaatgctga gctttcactg cgatgcgtgt cttttcgttc gtccattgct
    44041 gcgatgacag cagctttgat tgcttctaca ctatcagtca ctaattcatc aatgattttt
    44101 gattctaccc gttgacgaga tcctaatgta ttccaaatca agtctttgtc actagaatca
    44161 aattctcgaa cgcctgcttc ttgttcaata acttctggtc cattcaaacc gactctcgct
    44221 ttttttgttg cgattaaata actcattaat gctgaagtaa tcgacatacc gccaaatgat
    44281 cctacacgtc ctggaatcaa accgatgact ggtacatatt gttttagagc aatgattaca
    44341 tttccgattt ctgaaataga taacagtccg tagtttgctt cttgcaaacg aacaccacct
    44401 gtatctagta caatgactgg aaagatctca tttccttttt tattttcttc taacacatgt
    44461 tcaagtgcag caacgatttt tgcaccggaa acttcaccga taccgccacc ttggaatttt
    44521 ccttccatag cgattactac tgcttgtttg ccattgatcg tgccacggct gacaacgatc
    44581 ccgtcatcac tttcagggac gattccttgt gctggtaaac ttggcgcgat gatttgatcg
    44641 aatggatcaa tcaattcacg tccattgtca agtagagcgt tcactcgttc tctggcgttt
    44701 aattctacaa aactattttt catctgaaag ttcctccatt gcttgcgtca atcgtaggta
    44761 cacaactcct ggtgtagcac cgaagtcatt gatcacgata tctgctgaaa ttggataacg
    44821 atcaaaaaat ctcgttaata cattgttcca tacttcgccg aagccatcgc ttcctgtaca
    44881 aatttttaca gtagattctg ttttttctgt tggaaacatc aagatttcca aatctcctga
    44941 accgaccact cctacatgga ttttcttatt gatcggctgt ttcgttggat atttataagc
    45001 taattgttcc atggatctct cccctttttg taaaacagag catacacgtt ctgcttattc
    45061 gtctttcttc ttccatctgg gtaaatggcg acaaaaggtt tttctcgcca attttatgac
    45121 caagttctga atttcattgg tggttcatac aggccatctg accattcgat caaatcatcg
    45181 atggtttgtg cagctaataa tgaacgtttc gcttcactac gtttgatgcc tagatcttct
    45241 ggtaatttca cgatgccggc tgcacgcaag ctatctagtg attctttcgt gctagtcatt
    45301 ccaagaggtg taacaccgcc aattgctgca attgctgctt gtcgttcttc ttttgttttt
    45361 gctttgtaca aataagcaat tccttcttct gtcacgatat gtgtagtatc ttcgctataa
    45421 atcatcactg gtgcattggc taagcctgct tgtttttgaa cagctagtgc atctaattct
    45481 tcgacaaaga ctggtttttt atttgaacca tatgtttcaa ccatttgaac aactaatttt
    45541 tggcctttcc ctaatggatc atcattgtcg cgaagtgatt gccatgctgg tgtagaatga
    45601 cgacgaccgc caggattatg tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct
    45661 tttgttacgg tagaagagtt tccttcgtaa tctaattgca aggttgaacc gataaacata
    45721 tcgatggcat attgtcctgc agcttgtgat aatgttcggt tactgcgcat cgtgccgtct
    45781 ttaccgacaa aaaagacatc tgggcgagca gcaatatatt tttccatacc aacttcgcca
    45841 ccaaaactat ggatgctttc aacccaacct gattcgatgg ctggaatcat cgttggatgt
    45901 gggttcaata cccagttctt cgcgattttc ccttttaacc ctagtgattc accataagtt
    45961 ggcagtaaca attcgatagc tgctgtattg aatccaatac cgtgattcaa agattgtacg
    46021 ccgtgttctg cgtagattcc tttgattgcc atcattgcca ttagaatttg caattctgtg
    46081 atgttttgag gatctcgtgt gaataaagct tctaattgat aaggtttatc tgctttcacg
    46141 acaacgtcta cccagtcacc agggatatct acacgtggta aagaatcgac aacttcgttg
    46201 acttgaacga tcacgatacc atctttgaaa gctgcagctt cgatgattgt tggtgtttct
    46261 tccgtattga aacctgtgta caagttgccg tctttatctg ctttatctgc agctactaaa
    46321 acaacatttg gaatcaaatc gatgaataag cgtccataaa gttccaaata agtatggata
    46381 tcacctaatt tcattgtgcc gtcttcaatc atttgggcga cacgcaagct ttgtgcacca
    46441 gcataagaga aatcgatctt gcttgcgatt cctttttcaa aaatattcaa atgttctggt
    46501 cgtgagatac tagacatgat caaatgcaga tcattcactt tttctgcatc tactttttca
    46561 agactttctg aaagaaaact tgcttgtttt tgattgtcgc cttctaatac aactttgtcg
    46621 ccgggagcaa tcagtttttc cattacttct acgatttttt cagtttctac atacttgcca
    46681 ttgagcaagt tgctgatttt ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag
    46741 ttgcggtttg gtttttccat aattagtcat ctccattttt gatatagaaa cgtggcgtca
    46801 tcaataccat gttggtctag attgatcgta cggatccctt tatctacacc aaattttgta
    46861 tgaatcagct ttgtcaaagt attgccgggg ggaaattcaa tcgtcagatc cattcctaat
    46921 tcttttgcca agtccatcat ttggttccac tgcacaggga aagctaggtt ttccaacaaa
    46981 tctttacgaa tctcttctgc gtcctgagtg gctcttccat ccgtattttt tatatacaaa
    47041 catctagcat cagatagatg gacagactgc atatacggct tgaatttctt gaccgtatca
    47101 ttcatcaaga gacagtgaga tggcacaggg actttcagca gcttcgctgt ttgcgcttgg
    47161 tgccgtttgg cttctgccaa cgtaacggat aaaccttcga tactcccaga taacgtatgt
    47221 tgcatcggac aattttcatt tgacagataa actggatggt caggatcaaa tgtttttgct
    47281 acaatttcct cagcttcgct tctcgtcaca cctactacta cgcccattgc ataaccgctt
    47341 ggataagctt ctttcattaa gactgcgcgg tgatagacta gttccgctgc attctcaaat
    47401 ctcaaagatc gacaagcaac agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata
    47461 tcaggctgaa taccgttttt ttccatttca tctaaataaa atactgcttt tgtcagtaat
    47521 gctagctgga taaaaaccgt atcttgatag ttttcttctt tatcaactaa ccgatacccc
    47581 gtcacatttt ctaaacgttc catgtacatt tcagggatat ccgctaacat ccctaacttc
    47641 tgactgcctt gtccaggaaa taagaaacct gtactcatga gcctcgcctc ctcagcactt
    47701 actttttcca ctgctatctt agtcaatttc tgctataata aaaaatgcca aaaaaagaaa
    47761 aaaccataac aaaaaggtat ggatgggatg gataattatg aaattgcttc atctgcagta
    47821 ttttaaaaaa gtattcgaaa caaaaaatgt tacccaagca gccaaagaat tatttatttc
    47881 tcaacctgcc ttgagccgtg cgatcaaaca tttggaaaac gaattaggtg tccctttgtt
    47941 ttaccatagc ggcagaaaca ttgagacgac cgtttatgca gaagaatttt atccatatgc
    48001 agtcaagaca ttagacacat tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa
    48061 gatcgttact tccgtcatat tgtatttaga ggtagcctct gtttctattc cgaacttggt
    48121 ccgaattttt atggaaaagc atcctgatat ccaattaacg atcatgcaac atcatttacc
    48181 agatgatatt accgatcctg tactgtacat cacttcggag atgaagcctg gcatgaccaa
    48241 tattcctatc atgaaagaac ctgtttatgt cgccattcct aagaaacatc ctcttgctaa
    48301 aaaagaacat ctgcaattag aagatatcca acgcaccccc atcttgatgc tgtcaaagag
    48361 caacgccttc cgtcatacac tggatgcagc acttgaagcg aaaagtatcg aattgacgat
    48421 cagttcgacg acagatgatc ctgcgacatt acgttctatt gtcaagcaag gattagctgt
    48481 tagctttttc ccaaaagttt cttggtctta cgataaaaat gatcctttcg tcttgcggga
    48541 aatcaaagat ttgcccttga cacgaacaat ttatttatct tctagcttca cagaagatca
    48601 tcctttgatc aaaatgattg caaaaacgat gcgggaattt tatttaggaa aataaaaaaa
    48661 aaagagctgg gacaaaattc ttgtcgcagc tcttttttcg aacaaacggt gttccagaag
    48721 taatttctta gaaaataagc cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt
    48781 ccttcttatt tcttgaagct aaacatttct gtcacaacct cttctctatt gtcctagaca
    48841 ttcattgcgt tgatttaaaa cttccatcaa tcaaatctgc tagttcatct agcttttcta
    48901 ttttacgtag atcaatagag atccaatgtt ctttattcat gtgataggct ggaaaatacc
    48961 cctcttttcc tcgcaaaatt ccgccaagtt caggatcgac cttcaaatca agaatattga
    49021 tgatcctatg gtcacttgca tcatcataga ttttttcttc tggaacagac atcaggaccc
    49081 caaaccattt tttgcttttt tgatgtctga atacgcaata atcaggaaat tttttaaaaa
    49141 tatattctgg agacaaattg tatgtttcat tgatgtgatc gattagttct tttttcgtta
    49201 gcatctgctt ccatctcttt cttattgatt cgctttattc aaagatacca cgtaagaata
    49261 gaaaaatgaa tacaagtagt tgctaatcaa atacttcgat atttttcttt tttatcaact
    49321 cttaaatgtt caatccgcct gtgttggtct tgctaccatc tactattaaa atgtttatta
    49381 ttgtttgccc acctattttt tgattttagt ggtatcattt ttcaagttca tgatgaaatt
    49441 cttatcatca gtaaatgctc cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg
    49501 accaccgttt attttctccg tattcttaga atagaaggga gtctgattga tgtattcact
    49561 aatgaaaaaa atcattaccg aaaaagatat tcgacgtcat gtttctttag tagaacaatt
    49621 gctgaatcat acaaagataa ccgtaaatga attagctgaa atcataggga caaccgaacg
    49681 aacgattttt tctgaccttc aatctattcg ctctcagctg cccgaaggct gggacatcat
    49741 cagcgatcaa gcaggaatta gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg
    49801 ggagattttt ttcaaacaat cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa
    49861 aaaagttgct gtccctgatt ttctagccga ccatgggctt tcttatggga cattgaaaag
    49921 acatgtgact aaaatcaatc agcgtttagc aagttacgac ttgcagatcg atttgacgaa
    49981 atatacagct tgtattcttg gaaaagagcg tgcgattcgt actttttatc atcgattgct
    50041 gattcctttc actcataaca actatttttt cgaggattat tcgatccatg aatcccatta
    50101 ttttcaattt ttaagaaacc tcagtcaaac tgaactagcg gttgaaacag aagagatttt
    50161 cggaacatgt tggttcttta tcaatacgat ccgtataaaa gcgaattgcc gattagattc
    50221 ttctatccat accaacagca cactctcctc cctgtatgac tctgcattga aaaaattgta
    50281 tttaacggaa ggtatttatc ttaaagacac tgagctttct tttgcttctt tctgctttct
    50341 ggaaagttgg aattacaaca ataactatgg acaagaaacc gctcgctgcc tgcatcattc
    50401 tccattttta gaggtcctcg aaacgtttgt agaggaacta gcttcagagc tgtctcttga
    50461 tcaactaaaa aaaacatctc tgactgataa cttggctctc ctcatattaa aatatcatga
    50521 atcgccgata ttgattgaag cattggaccg tcaataccat cgttttttag aaacatatga
    50581 tcagcaatat gcacacttgt atcaacagaa agagaattta ctgaacgatc taaaaaaaga
    50641 aatagaaatc aatgaaccaa attactttct ccaactcttg tctctactga tccaacaaac
    50701 gattttttct atcaaaccga atttgttcaa tgtctatttt attttccaag gagaaccttc
    50761 atggaaggcg ttcttgcagc aggaattaaa agattactta ggaaaacgtg tctgcttctt
    50821 acctatcgaa ctaaccgatc tttccactat ttcttttgaa aaaacagata tcttgatttc
    50881 caatttccct ctagattctc tagatatccc tattgtatac atttcttcta ttccaacgaa
    50941 aaatgagctc aatcgtttga ctgaactcac tttcaaaact tttttatagt aacggctagc
    51001 gactgtattc tctagctgtt ttttcttttt tgcgaaacag tttgttcatg atcccttttt
    51061 ctatttctac aactaagaat agaagaatca catttgctcc aatcaataac tgttgtgtca
    51121 tcgttaaact tactgtaccg atcagctgct ggccgactgg tagaaaaata accatccctt
    51181 gcaataaagc aaggatacct aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt
    51241 tcaatgacgg cttggacaac tctctgcaat tgatcatgta aacagcttga gctaagacga
    51301 tattttgcag aagaatggtt tgctgtaagc tttgcccttc aaactgcata gccaataaat
    51361 aggcaggaac catgatcagt agggagacat agatgatccg aaacaaatga taaccagata
    51421 ggattccttg ctttggatct cttggtgggc gcttcatgat ttctggatct ggcgactcga
    51481 accctagttc ataagataaa gtgattgtcg ttaccatatt gacccataaa atctgtactg
    51541 gggacaaagg tagcgggcga ttcagcataa gtgcccaaat caagatcaat ccttgagcta
    51601 atgcagttgg caagaagaaa ttgatggttt tttgcaaatt atccaaaatc cgccggccct
    51661 cttttacggc ttttgcaatc gtatggaaat tatcatctcc taaaaccata tcagctgcct
    51721 gtttgcttac ttcactacct ttgattccca tagcaattcc tacatcggat cttttcaaag
    51781 caggtgcatc atttacgccg tctccggtca ttccgacgat ttctccattt ttctgcaaag
    51841 ctttaacaat tcgcagttta tgttctggtg tcgttcgagc aaagacatcg actattggca
    51901 cttgctgaat caactcttca tcagacataa gatccaaatc gatcccttct aaaactttgg
    51961 cagtatgttt taagccaatt tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg
    52021 taatcatttt cactgagatc cctgcttgtt ggcattcctt tacagctttg actgcacttt
    52081 cttttggcgg atcgatcaat ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat
    52141 gcgtgagttc ctgtttcgaa tctacttttt tataagcaaa tcctaaaaca cgctgacctt
    52201 ttctcgcaaa ttctgcagct gtctgactcc atgcttcttg ttctgcagaa gaaagagttg
    52261 atttagcaaa taatacttct ggtgcacctt tgacgtaaat caccgctcct ttttcctccg
    52321 catgcatcgt tgccatatat ttgtattcag aactgaacgg aatctttgct tctatttttt
    52381 tcaaaggtag attggctgtt tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac
    52441 ttaaattttc tgttttctgt tcttcttttg tttttaattc ctgacagttt ttcataatgg
    52501 acaacacgca ggcttcttct tttgcagcta catccacaac tgtcatttca ttttttgtca
    52561 atgttcctgt cttatctgaa cagatcactg tcatagaacc taatgtttct accgaaggaa
    52621 gtcctttgat gatcgccttt tcttttgcca tttcatgtac tccaacggat agaatcatgg
    52681 tcaaaacagc tggcaatcct ttgggtacca ttgacaccac caaagcaatc gtagccgaaa
    52741 gaaggatatg ccaatccata ccatatcgaa atgtcgtaaa aataactaag aataggctca
    52801 aacaaagaat gccttgaaaa atttgtttgt ttaatcggtt gattttttta atcagaggcg
    52861 tcgtttgttg ttcgaccgat tgcaatgctt gattgatttt accgatttct gtcgtgtttc
    52921 ctgtttctac aacgacaccg actgcagaac cagattgtac aagcgttcct gaaaaagcca
    52981 tatttttctg gtcgccgatc gattcttctt ctgtcaaaac ggcatgactt ttttcagatg
    53041 cttctgattc tcccgtcaaa atcgattctt ccactaataa atcatgtgct tcgattaatc
    53101 ggatatctgc tggaacgaca tctccggcac gtaagacaat gacatctcct tttaccagtt
    53161 ctttcgcagg gattttttct tgcatcccgt ttgatagtac tatcgcttct tgtcccatca
    53221 gctgcttcaa accattcaat gattcttccg cttttctttc ttgccagtaa ctaacgagtc
    53281 cattgacgat cactacgaat aaaataatac ttccttcaat atattcacta gaagcaaatt
    53341 tcaaaaaaga ggcaatgatc aacacgatca tcagcagatc agtaaaatgc tttgctattt
    53401 ttttccatcg ttttgtctgc tgccttgctt ttatttcatt atatccttga tcagtcaggc
    53461 gtttctgtcg ttcttcttgc gacaagcctt ctgttgatgt ttgatagttt ttcaatacat
    53521 cttctaccgt caatctgtac cagttcatcc tatcccctcc tgtttaattg atactgttat
    53581 tatacgcagg gaagtttttt caggcgtatc gattatttca ggggggtctg aaataattcg
    53641 ttttgcaaaa tgtatctggg cctaactgat atttctattg cttattcttt tatatacaaa
    53701 atttaagaat acaaaccaga taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa
    53761 taacaagctg aaagcaattg aagtaaaaaa caccaacaaa gcaagatctt tatatctttg
    53821 tgtagtgttc cgtttctttt ctttaataaa ttgaacatgt gcacacaaga tcgaagacca
    53881 cacaaataga aaacaactcg ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc
    53941 agctacaaaa aaatgtagac aaagaccggc aaaaaataac attcctgtaa ttaaaatacc
    54001 gtgtaaaggt acgcctttct tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt
    54061 tgctgtttga tataaaatcc tgctagtact gtagactgca ctatttgctg aggacaaaga
    54121 agcaaccaaa atcattagat tgacaattgc tgctccgcct tggcaatgga tcacttgcag
    54181 catagtcact aaaggacttt cttttaaatc taaacaatgc catggaacaa caaataaaat
    54241 cgacaaaatc gttcctatat agaaaaagag aatacgccaa ggcagacagt tgattgcttt
    54301 tttcaaattc ttttgcggat cacttgtctc gccaacggtt aagccgatca tttcaattcc
    54361 agcaaaactg aataaaacca taggaaatga gactaccatt ccagcaaagc caaacggtag
    54421 taattcatcg atctgtccaa aattttcttt gattccgata gactgggtat gaaaatctga
    54481 gacactcaat cctataccca ataagataaa aaggaaaatc cccaagatct tgatgattga
    54541 gaaaaaagat tccaattcac caaaccactt gactgcaaca aggttgactg ccattaagat
    54601 cattagtgcg ctacctccgc ttatcaaagc tggtacatgt acaaaccaga aatggatata
    54661 caatcctatt gctgttaatt ccgcaagtcc agctccaagc cagcaaagcc aataagtcca
    54721 attgacaaaa aatgcccact ttttgcctaa ataatagtca acgaaatcac tgatagagtt
    54781 aaatgtacta tttgctaaaa ataaatcacc catggcccgc atcaaaccat aaatagccaa
    54841 accgcatatc aaataaacga gaataacagc tggccctgct gatgcaattg cctttgctga
    54901 acctaaaaat aatcctgttc caattgcgcc acctaatgcg attaactgga tatttcgatt
    54961 agttagagtc cgtttcattg ccggtttttc catcaagttc acctttttct ttttcaagca
    55021 gcatggcgac ttcctgttcc caagaaggtt tctttatttt ttcaatacag agtggtacta
    55081 caaacaaaat gacactgatg gctgtgactg caattagatt gatccatgtt ccagctttca
    55141 tcgtcagtcc gacagaaaat ccgattgccg caaataaaac aataacgaca gtccaaactc
    55201 caagattgct ctttttctct ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg
    55261 tataacgcaa tctcaaaact gcgatcccca tgatgacata gacgatacag tagataacag
    55321 tagttgaatt tacgagcatc aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa
    55381 gtgcaaagat tgagataatg acagactgag tcaaaataac ggatttggat acgtcaaatt
    55441 gattcgtctt ccaaaaattg aattttggtg gatacgcacc ttttctagca ctttctgtca
    55501 ctgtctttgc tggtcctgag gcccaagcag ataattggac tgctacacca atgaagacaa
    55561 gtaaactaaa cagattcaca ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa
    55621 tagattgcgc gatattattc aattccattt gtccttttgg tacgacattt gctacaacta
    55681 aagcattcaa aatattgacg aaaaatataa aaattaacgc ggcaaatact ccgcgaatat
    55741 acgttttaac tggattttcc aatcgagtga tatacacaga ggacatttcg ataccagtaa
    55801 aaataaacat gatcggcgca aaataaacaa atgaggatgc agtcgtagta tctggtatta
    55861 atttgtccca tgaaaaactg cctaatgttc cggttggtac gatcccaact ttgatccacg
    55921 cagcaaaccc taataagagc atcatcgtca aaggcacgta caatcctaac caaacaccga
    55981 cttttccgcc gatttttgcc atatcaaatt tcaaattcaa aaaagtgatg atccaataaa
    56041 cgactaaaat gacaactaat gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa
    56101 tattaccaaa caacggagcg agtgctgaag cgaccatcac catcccagga aacatttgga
    56161 cccataacag ccatgagaca acgaaccccc atttttttcc tagagcattc gatacccaaa
    56221 gttctggacc acctttttgg ggaaacattc cagcaaattc accagagatc aatgagattg
    56281 gaaaagcgta caataatact gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg
    56341 ctacatctgg aatatttcgt acacttgcta caagggcgca ggtcattccg ataaaggtca
    56401 tggtcgtgat ttgctttgat gaaccagtat ctttcatctt tattccttct ttcaatagcg
    56461 acatatgttt ttgttttctt ttatttttgt tcaagaacag aaatgacaag gtttcatcga
    56521 ttcagtacaa aacccagtat caccgaaaca tccattgaac gatcttagtg aactgggttt
    56581 gatacatgaa acgaacgatc caacttatag aacgttttat aaaaaagaat atcttggcga
    56641 ggaatatatg aaaaacaggg ggaaatacaa ttgttttgat gaagcaaata attgtctgtt
    56701 ctccatatgt ctcttgttat tctttcacac aaaaatcagt tctattggat gatttcgttt
    56761 agtttctttt ccattgcttt ggcgattttt tttgtataaa aattgaaaat tcgatcatca
    56821 ttcaaataag gagtcatgat agccgcacgt agcagagtga ttttctgttc ttttttccat
    56881 tcttctttcg ttagacccat tctttcaaca aaacgaatcg gagaatcacc gtaatcttct
    56941 tgtgtaaatg tagtatgaga agtaataaac cgttcaccat aaacatcgcc atccatataa
    57001 gaagaccgat cgaacatttt ttcatttaat tcattgatag cgttcaaatc tgtacagcct
    57061 tgttctttga atacccaatt gaccatatta aaatcaggat gattcaatgg atagacttcg
    57121 attgtttttc ctttaacagt aaaagtcaga tgatcgagaa attctctgaa tctttgagct
    57181 gcctcgattg aagccccaat caattgacca tatccggtca cattcaatgg caatacacga
    57241 tgtgctgtcc agactgcggc ggcggttgct ccagcttttg atccttccaa aatataagct
    57301 cctaacatat ctggagcctt tactgatttt tcaaacacat atggcgcgaa ataagaaatg
    57361 atatttctca tatttttatg tttgattacg attcctccag cagcataagg gacatagccc
    57421 atcttatgag gatctatcgt gacagaatcc gcttctgaaa tagctctgaa cccttcatat
    57481 acctctttgt caatcgttac gcagtggtgg aaaacatgat gttcttcaaa gaattcagct
    57541 aaggaagcat acggcacaaa ttctcctgct tcgtttagaa acagtgaacg cgcatagccg
    57601 ccatatgcgg catcaacatg taaatagaaa tagatccctt catcttttaa cctttctctt
    57661 aattggacga ttttatctac actgtctact tgaccttctt ctgtcgttcc tacgacagca
    57721 accacaccta gaattgggat tttttgtcca gctaattcac gaattgtttt ttccaaagca
    57781 ttaatatcca ttcgataatc ctcttgaact gggatagcaa ccatctgatc taagccgact
    57841 ccgcaaatat ctaacgcctt catccaagaa tagtgttttg tttgagggac cagccatttc
    57901 cctaatcttt ggatattttt tccgctacgg gaagaagctg ctttgacctc atccatttct
    57961 tcgtccgtaa agctttcggt catttccaaa atttcttcaa cagataaatt caaaagttcc
    58021 cactcagaca tttttttcac tttttcaggc agcacttcct tgacagctaa aggaatcgat
    58081 ttgatgcaac gggcgtacca taatccttca agattagcaa tagaaccatc tgctgcaatg
    58141 tgtccccatc cgtctgtcat attgaataat gaagcaaaat cctgtcctac ttctgcttcc
    58201 atttgcgaag ttgccataga agattccaat gcaacattat ttgggttcca cagcatcgca
    58261 tagttgtaag ctaataaagc aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg
    58321 cctgctgaat gccatgggat cgatcctgcg cgtaatcgct gactgacttc atccaagacc
    58381 tctatcatat ggtcgcgggt ggctagataa tctggagtga gcttatctcc ttcgctaatt
    58441 gcaggcatat ctgaaggaat ataattttct ctccatccaa gatgttcatc taccatcttg
    58501 ttcaacaaca ttttataaac tggtccattt tcagctttgt cgcctataaa gacggccttg
    58561 atatccatat ctttcatttt tcttccctcc atattttgtt ggtcaatgtc ttgcagatac
    58621 atgtgtttat ctgtaacacc tcttcactat agcgccaaaa atgagaaatt tttttttata
    58681 acatcaaatc cacgtagatt tttacaaaca aaaataaata aagtcggatt tttctgataa
    58741 ttcaa
//
LOCUS       DADXTG010000003        52774 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.003, whole genome shotgun sequence.
ACCESSION   DADXTG010000003 DADXTG010000000
VERSION     DADXTG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.003"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..401
                     /locus_tag="IWI57_000113"
     CDS             <1..401
                     /locus_tag="IWI57_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448289.1"
                     /translation="NIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSI
                     SGYVVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVL
                     LIFLFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            423..1934
                     /locus_tag="IWI57_000114"
     CDS             423..1934
                     /locus_tag="IWI57_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBC2448290.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            1946..2905
                     /locus_tag="IWI57_000115"
     CDS             1946..2905
                     /locus_tag="IWI57_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBC2448291.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            2991..4070
                     /locus_tag="IWI57_000116"
     CDS             2991..4070
                     /locus_tag="IWI57_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2448292.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(4322..5722)
                     /locus_tag="IWI57_000117"
     CDS             complement(4322..5722)
                     /locus_tag="IWI57_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBC2448293.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            5940..7601
                     /locus_tag="IWI57_000118"
     CDS             5940..7601
                     /locus_tag="IWI57_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBC2448294.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            7558..8256
                     /locus_tag="IWI57_000119"
     CDS             7558..8256
                     /locus_tag="IWI57_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBC2448295.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            8272..9696
                     /gene="araA"
                     /locus_tag="IWI57_000120"
     CDS             8272..9696
                     /gene="araA"
                     /locus_tag="IWI57_000120"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBC2448296.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            10003..11163
                     /locus_tag="IWI57_000121"
     CDS             10003..11163
                     /locus_tag="IWI57_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBC2448297.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            11281..13140
                     /locus_tag="IWI57_000122"
     CDS             11281..13140
                     /locus_tag="IWI57_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBC2448298.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            13250..13684
                     /locus_tag="IWI57_000123"
     CDS             13250..13684
                     /locus_tag="IWI57_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448299.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(13943..14104)
                     /locus_tag="IWI57_000124"
     CDS             complement(13943..14104)
                     /locus_tag="IWI57_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448300.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(14123..15145)
                     /locus_tag="IWI57_000125"
     CDS             complement(14123..15145)
                     /locus_tag="IWI57_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBC2448301.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            15273..15626
                     /locus_tag="IWI57_000126"
     CDS             15273..15626
                     /locus_tag="IWI57_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBC2448302.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            15638..16804
                     /locus_tag="IWI57_000127"
     CDS             15638..16804
                     /locus_tag="IWI57_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2448303.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16896..17516)
                     /locus_tag="IWI57_000128"
     CDS             complement(16896..17516)
                     /locus_tag="IWI57_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBC2448304.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(17708..18004)
                     /locus_tag="IWI57_000129"
     CDS             complement(17708..18004)
                     /locus_tag="IWI57_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBC2448305.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18208..18858
                     /locus_tag="IWI57_000130"
     CDS             18208..18858
                     /locus_tag="IWI57_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBC2448306.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18874..20601
                     /locus_tag="IWI57_000131"
     CDS             18874..20601
                     /locus_tag="IWI57_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448307.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            20598..22490
                     /locus_tag="IWI57_000132"
     CDS             20598..22490
                     /locus_tag="IWI57_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448308.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            23194..24063
                     /locus_tag="IWI57_000133"
     CDS             23194..24063
                     /locus_tag="IWI57_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBC2448309.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            24272..25297
                     /locus_tag="IWI57_000134"
     CDS             24272..25297
                     /locus_tag="IWI57_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBC2448310.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            25336..26286
                     /gene="manA"
                     /locus_tag="IWI57_000135"
     CDS             25336..26286
                     /gene="manA"
                     /locus_tag="IWI57_000135"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBC2448311.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            26380..28083
                     /locus_tag="IWI57_000136"
     CDS             26380..28083
                     /locus_tag="IWI57_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2448312.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            28085..30118
                     /locus_tag="IWI57_000137"
     CDS             28085..30118
                     /locus_tag="IWI57_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBC2448313.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            30129..30569
                     /locus_tag="IWI57_000138"
     CDS             30129..30569
                     /locus_tag="IWI57_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2448314.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(30769..31896)
                     /locus_tag="IWI57_000139"
     CDS             complement(30769..31896)
                     /locus_tag="IWI57_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448315.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(31899..32576)
                     /locus_tag="IWI57_000140"
     CDS             complement(31899..32576)
                     /locus_tag="IWI57_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448316.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            32840..34606
                     /locus_tag="IWI57_000141"
     CDS             32840..34606
                     /locus_tag="IWI57_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBC2448317.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            34621..35058
                     /locus_tag="IWI57_000142"
     CDS             34621..35058
                     /locus_tag="IWI57_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2448318.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            35071..36222
                     /locus_tag="IWI57_000143"
     CDS             35071..36222
                     /locus_tag="IWI57_000143"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBC2448319.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(36284..36982)
                     /locus_tag="IWI57_000144"
     CDS             complement(36284..36982)
                     /locus_tag="IWI57_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBC2448320.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(36975..38327)
                     /locus_tag="IWI57_000145"
     CDS             complement(36975..38327)
                     /locus_tag="IWI57_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBC2448321.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(38550..39950)
                     /locus_tag="IWI57_000146"
     CDS             complement(38550..39950)
                     /locus_tag="IWI57_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBC2448322.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            40111..40848
                     /locus_tag="IWI57_000147"
     CDS             40111..40848
                     /locus_tag="IWI57_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBC2448323.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            40973..41452
                     /locus_tag="IWI57_000148"
     CDS             40973..41452
                     /locus_tag="IWI57_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBC2448324.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            41449..42216
                     /locus_tag="IWI57_000149"
     CDS             41449..42216
                     /locus_tag="IWI57_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448325.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            42446..43015
                     /gene="lepB"
                     /locus_tag="IWI57_000150"
     CDS             42446..43015
                     /gene="lepB"
                     /locus_tag="IWI57_000150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBC2448326.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(43119..43721)
                     /locus_tag="IWI57_000151"
     CDS             complement(43119..43721)
                     /locus_tag="IWI57_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBC2448327.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            43827..44296
                     /locus_tag="IWI57_000152"
                     /pseudo
     CDS             43827..44296
                     /locus_tag="IWI57_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            44402..44986
                     /locus_tag="IWI57_000153"
     CDS             44402..44986
                     /locus_tag="IWI57_000153"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBC2448328.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            44989..46062
                     /gene="prfA"
                     /locus_tag="IWI57_000154"
     CDS             44989..46062
                     /gene="prfA"
                     /locus_tag="IWI57_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBC2448329.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            46055..46894
                     /gene="prmC"
                     /locus_tag="IWI57_000155"
     CDS             46055..46894
                     /gene="prmC"
                     /locus_tag="IWI57_000155"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBC2448330.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            46887..47066
                     /locus_tag="IWI57_000156"
     CDS             46887..47066
                     /locus_tag="IWI57_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448331.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            47088..48113
                     /locus_tag="IWI57_000157"
     CDS             47088..48113
                     /locus_tag="IWI57_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBC2448332.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            48288..49532
                     /locus_tag="IWI57_000158"
     CDS             48288..49532
                     /locus_tag="IWI57_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBC2448333.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            49685..50632
                     /locus_tag="IWI57_000159"
     CDS             49685..50632
                     /locus_tag="IWI57_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2448334.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            50766..51395
                     /gene="upp"
                     /locus_tag="IWI57_000160"
     CDS             50766..51395
                     /gene="upp"
                     /locus_tag="IWI57_000160"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBC2448335.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(51534..51776)
                     /locus_tag="IWI57_000161"
     CDS             complement(51534..51776)
                     /locus_tag="IWI57_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448336.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            51897..51969
                     /locus_tag="IWI57_000162"
     tRNA            51897..51969
                     /locus_tag="IWI57_000162"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51930..51932,aa:Thr,seq:cgt)
     gene            complement(52088..52726)
                     /locus_tag="IWI57_000163"
     CDS             complement(52088..52726)
                     /locus_tag="IWI57_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBC2448337.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        17628 a         9330 c        10899 g        14917 t
ORIGIN      
        1 gaaatatcat ttattttttc tttcaaggaa taaagcaaca ttttggtaag aacttgttcc
       61 ttagtctgtt aacgatcatt atttattttc ttgttaatca attattagaa gaacagacaa
      121 tcgtactttc aattagtgga tatgtagttt tacttattta ttccatgttt cttttatcct
      181 ggatatatgt gatttctgaa aacaaagata tcacattttt acaaattttt gaacaaagtg
      241 catggaaagt attttttgat tttttgagaa atattctttg ttgcgtattg ttattaggat
      301 ttgtactgct catatttctt ttccctcctt ttatttttat ctttgctggt ggcgtatgga
      361 aacttgtatc tattatcttg actataggaa atgaaaggta gggaaagctg ttggacgaag
      421 aagtgaaagt aaaaatcaga gaggatttta cggtaggaga gatcagttct cggctgtttg
      481 gctcatttgt tgagcatatg ggaagtgtcg tctatacagg gatttatgaa ccgggccatg
      541 aaacagctga tggtgacggg tttagacaag atgtcctctc ttatgtaaag gaattagaat
      601 taggtgtcat tcgctatcct ggaggaaact ttacatctgg ttatgattgg gtggatactg
      661 taggtaggaa agaacaacga ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa
      721 attcttttgg actccacgaa ttttttaaat ggatcagtca tgtacaagcg aaaccgatat
      781 tcacggtgaa tttaggaaca aaaggaattg atgaagcaaa aaatttagta gaatattgta
      841 attttccaaa aggaaccttc tggagtgatt taagaataaa aaatgggcaa aaaaagcctt
      901 ttggcgttaa attatggtgt ttagggaatg agctagacgg tccatggcaa gtgggggcta
      961 agagtgctta tgaatatggt aggttagcaa atgaagcctc aaaagtcatg aagttagtgg
     1021 atgattcaat tgaaacaatc ttagttggaa gctctacgcc acgtttacca tcgtaccctg
     1081 aatgggatcg tatcgcatta gaagaagctt atgagaatgt agattattta gctttgcata
     1141 actatattga taagtatgat gaagaagact tgactaaacc tcctaaaaat gagcgtgatg
     1201 atacagcgac ataccttgca aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg
     1261 ctacttgtga ttatgtaaaa ggatatttga gaagtgagaa aacaatgtat ctagcgtttg
     1321 atgaatggaa tgtacatgcc caaccggaaa aaagacatag ggattttgaa gtcggtagtc
     1381 ccatcgattg gtgttacttt actatggaag atacattatt atttggttca ttagggctgg
     1441 ctattttacg acacgcagat cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa
     1501 tacctttaat actgacagat gctaaaggac aagcatggag gaatccaact tattatgtga
     1561 tgcaacactt atcgaaatat gctaaaggtc aagtattgaa acaacaaatc atttgccctg
     1621 tttatgattg tgaaaagata aaaaatgttc cttatgtgga cagtgtgatc gtagataacg
     1681 gcaatcagct gactattttc ttgatcaatc gcacaaatga acaacaaaag ataacgattg
     1741 aacatgattt caatttgcta gatcaaggag agcactacac attaaccagt agcgatctgt
     1801 tggacaaaaa tacacgtgaa gaaccagaaa aaataaaacc tgaaataaac tatttttcaa
     1861 tagaaaaaca tgacgaatta aatgtaggga tagaaaaata ctcttggaat gtaattagaa
     1921 taaagaaaat gtgaggaaat ttgttatgat gaagacactt tataataatc ctatcgttcg
     1981 gcaacgggca gatccttggg tatataaaca tacagatggt tattattatt ttacaggatc
     2041 ggttccaggc taccaagtga ttgaattaag aagggcaaag tcactgaatg aattagaaca
     2101 tgctgcaagc ctgattgtat ggcaggctca tgaagaagga ccgatgagtg aattgatctg
     2161 ggcacctgaa gtccattata tcaatggaaa atggtatatt tactttgctg caagcaatca
     2221 taaaactata cgtgatgaat ctcatcatca ccgcatgttc gtacttgaaa atcagcatat
     2281 agatccaatg aatacagatt ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt
     2341 cagtttagac ggaacaactt tctcttctca aggaaagcta tattatgtgt gggcccaaca
     2401 agatcctgaa attactggga attctaatct ttatctatcc gaaatggata atccttggac
     2461 gttaaagggg aagcagatcc tcctttctat acctgagtat gaatgggaaa aacggggttt
     2521 ttcagtaaat gaaggtccag cagtccttac tcgcaatggg aaaatattca ttacgtattc
     2581 tggtagtgca acagatgaaa actatgccat gggcctgctg tgggctgatg agcacaagaa
     2641 tcttttatta aaagaatcat ggcataaatt atcggagcca gtattcgttt cttctgaaaa
     2701 aaataagcaa tttggtcctg gtcacaactc gtttacggtt tcagaagacg ggtgctttga
     2761 tattttgatc tatcatgctc gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc
     2821 taatcggcac gcgaacgcgc aatgctttac gtggaatgag caaggatttc cagaatttgg
     2881 tgaacctgtt ccgtatgcct tatgagcaca agaaaccttg atttttgctt gaataaacct
     2941 tgcagactcg ttatgatata tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat
     3001 atcaagatat tgcagaagag ataaaacaaa agatccttcg tgaggaatac gaagtaaata
     3061 caaccattcc ctcagaattc aagctgcaag aaatatatga cgtcagcaga catacgattc
     3121 ggcaagcaat cgctgtgttg gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca
     3181 catatgtaga tgacagctat aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg
     3241 gtgtcatcac cacatatctc tctgattata tttttccgtc gattatccga ggaatcgaaa
     3301 atgaattacg agaatctggg tattcattgc ttttagccag tacgaataac gatcacgaat
     3361 tggagaaaga gtgtttgaag aaaatgattg cttatggggt agatggtttg atcgttgagc
     3421 ctacgaaaag taatcagtat aacccaaact tagcacttta tgttgctttg agagaacaag
     3481 gcattccgat ggtgatgatc aatgctgctt atgaagagtt aaatgcctct tatatttgtt
     3541 tagatgatgt gaaagtcggt tttatggcag cagaatattt gataaaaaaa ggacatcagc
     3601 gtctgttgtt catcacaaaa atcgacgatc ttcaaggaaa atacaggatg aaaggtttta
     3661 tcagcgcgtg tgaacagtat aatgtgcgtt ttttgacgga agatatcatt actttcacca
     3721 cggaaacacg agaaacaatc gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt
     3781 tagatggcat catttgctat aacgatcaaa tagctagttt aatgctagat aagttgacag
     3841 aaaatggcta tcatattcca gatgacctat cgatcgtagg aacagacgat tcttctttaa
     3901 gccatttagg gaataaaaaa ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag
     3961 atgccgcacg gtggatcgtc acaacgattg aaaaaggaca ggcacagcca aatattctct
     4021 atgaacctgt actagttgaa cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt
     4081 tttaccgcct cttattgctc ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca
     4141 tcgctcaagg ctgaacgttt ctttcaccac ctctccacta aacggtgttc tcctaccaac
     4201 tggtcacaag tcacaatatt catcaaacat gagaagctgt tttttgaaat tgttccttgt
     4261 gactggaaac taagcggttc cttcttattt cttgaagctg accacttctg tcacaacctc
     4321 tttaagaatg atggatagaa atacgtttag gataagtttt tttagatgca ctttgttcga
     4381 tttcctctaa agatttccca cgagtttctg gtacgatctc ttgaataaat aaaaccccta
     4441 aaaagcagat gatcccaaaa atgccgaaaa cgatttcttg agacatagaa gcagtcatca
     4501 caggaaaaag caagccaact aggaaagaac caatccaatt aaaagaagag gctaggccag
     4561 atgctcgccc gcggacagct agtgggaata tttcgcctac gattacccat gtcagtggag
     4621 cccatgtgca agaataaaat gcgacataaa tacataaaaa caagagaatc aacaacgaat
     4681 ttgtatgagc atccagtaca gttcctaaaa cagctggtag gatgaaagaa agtcccatca
     4741 cgctaccgcc tattttcaat aaagttcgcc gattgaactt gtctgcaata accaaaaaga
     4801 gcaaagaacc agcaactaaa atgattcctt ggatgatcgg ccacatcaat gcatcgctcg
     4861 ctgcatgccc agtcgctttt tctacgatca gcggaatata gtaaaaaata gcattagctc
     4921 cttgaaattg ctggaatact gctacaccaa taccggcaat gactaaggaa cgatattttt
     4981 ctaaaaacaa agtatgccaa gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga
     5041 tttgttcgaa ttcctgttca gcttcttttg gtgtccgaat atagttcaat actctttttg
     5101 cctcttccaa gcggccagat tgcataagaa aacgcggtga ctctggtaga gctaaaacac
     5161 cgaaaaataa aatcagcgcg gggacagcag cgagccctaa catcacgcgc caagccatcg
     5221 tttcaggcaa cccttttagc aaataatctg caacatagga aaggagcatg ccagaagcaa
     5281 tcatcacttg attgatccct gacaatcttc cccttagtct agcaggagcc atttcagaca
     5341 tataagcagg aactagggca gatgcagcgc caacagctaa acctagaaga atccttgctg
     5401 caatcagaaa ataagaacca ttgtgagggg cagatgcaga aaggacagaa ccagccataa
     5461 aaataagtgc agaaagaaga atcatttttc gtcgtccaag cttatcagaa agaagaccag
     5521 ataaagagcc gccaaggatc gcacctaaca tgaccgaaga cgtgatccat cctatcaaag
     5581 aagctttacc tgccaaaccc caatcatgct gtaaaaaagg taaggcgccg gtcataacac
     5641 caatatcata tccaaataga atgccgccaa atgcgccaaa aaaataaata aacgatgagg
     5701 aaactttttt ttcttttttc ataagtattc tctccttcta gaatttcgag aaggcggttg
     5761 atcagatttt gtaagcaatt tcattttata acttatgtac gtacaaaaca agtgtttttt
     5821 attgtattct tgtaaataac tgcttgacat gtacgtacaa aagtatacaa taatcattgt
     5881 aagcgctact aaaaagacgg tttgatcagg tttgacgaga aggagggagt taaatcatta
     5941 tgaaggaagt aaatgcgcaa agtatgttag atgtcataga tggtcgtaca tctttaggga
     6001 ttgagtttgg atcaactagg atcaaagctg tattgattga ttcatctttt actcctattg
     6061 ccgctggtag ttatgagtgg gaaaacaaac tagaaaatgg tatctggacc tattcattag
     6121 aagatatttg gaaaggactg cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa
     6181 tctatggcga gaatcttacg acaatcgggt cgattgggtt cagtgcaatg atgcacggtt
     6241 atctagcatt tgatagcaag gaacagttac tagtaccatt tcgtacttgg cgaaatacaa
     6301 tcactgaaga agcagaacga atattaacgg aaaaatttca atacaatatt ccgcagagat
     6361 ggagtatcgc tcatttgtat caatctattt tgaataaaga agaacatgtg aaagaaatta
     6421 attttttgac aacgcttgca ggttatgtcc attggcagct tactggtgag aaagtactag
     6481 gcgtaggaga tgcatcaggc atgtttccta tcgatccttc aacgaaaacc taccatcaag
     6541 aaatgaaaaa tatttttggt cagttaatcg aaaaagcgca cgtagcgatt gacttggatc
     6601 acattttgcc taaagttcta gtagcaggag aagaagctgg caagttaagc gaatcagggg
     6661 cttatctgat cgaccctacg ggaaatttga gaccaggaat cccggtttgt ccgccagaag
     6721 gggatgcagg aaccggtatg gtcgctacga acagtgtagc caaagggaca gggaacattt
     6781 cggctggtac ttccgccttc gctatgatcg tattagaaaa agaactagaa aatgtccatc
     6841 cggaaatcga catggtcact acaccttcag gagaactagt aggcatggtt catacgaata
     6901 actgctcttc ggatatcaat gcttgggtca gtatttttga agaatttacc aattctttgg
     6961 gtatggagat aagcagagat aaattattca cggtattatt caacaaagcg ctagaaggcg
     7021 atacggattg cgggggatta ctttcttatg gttatttttc cggtgaaaac attacaggag
     7081 taaatgaagg aaggccatta tttgtacgta caccaggtag ccgttttgat ttagcgaatt
     7141 tcatgcggat ccatttagcc agtgcttttg gtgcaatgag aatcggaatg gatatattga
     7201 aatctgagaa tgtccaaatt gatcgtttag tggggcatgg ggggattttt aaaacaccag
     7261 aagttggaca gcggattcta gcatctgcta tggaggcacc agttacagtg atggacacag
     7321 ccggagaagg tggagcgtgg ggaatcgcgt tattagcagc ctatatgatg gatcgacatg
     7381 ggaagtcttt agaaaacttc ttggcagaag acgtgtttgg acaagaccaa ggaatcacca
     7441 tcacaccttc agcagaagaa atagcaggct atcagatatt tatgagacgt taccgtgagg
     7501 ggatcgacat tgaaaaaaca gcgattcatc aattgcaaga aaaaggagtg atagaaaatg
     7561 ctggaacaac tgaaagaaga agtataccaa gcaaatcttg acttgcctaa acacggactc
     7621 gtcaaatata catggggaaa tgtgagcgct tttgatcctg aaacacgata tttcgttatc
     7681 aaacctagcg gagtcagtta tgaagagatg acagcagacg acatggtcgt tgtggacttg
     7741 gacaatcata tcattgaagg taaactcaat ccctcttcgg atacgcctac tcatgctgtt
     7801 ttatatcgtt catttcctca aatcggagga atcgtccata ctcactcgac atgggcgacg
     7861 atctgggcac aagcaggatt ggatgttcct gcgatgggga cgacgcatgc agatactttt
     7921 tatggatcgg taccttgtgc tcgatttttg acacaacagg aaatcgacag cgggtacgaa
     7981 tatgaaacag gaaaagttat catagaaaca ttcaaagaac gcaaaattga tcccttagca
     8041 attccagggg tacttttaca cggacacggt ccatttactt gggggaaaga tgcgcaaagt
     8101 gcggtaatga acgcagtcgt actagatgaa gtatgcaaga tgaatttatt taccagacag
     8161 ctcaattcat tttcagaaga gctgcctcaa cggattttag ataaacatta tttacgaaaa
     8221 cacggaaaaa atgcgtatta cggacaaaac aaataaaagg agtggctaat tatgttgaat
     8281 attggagaaa aagaattttg gtttgttgta ggatcacagc atttatatgg ggaagaagca
     8341 ctgcgagaag taaagaaaca tgcacaagaa atggtcgatg aactgaatga aaatggccaa
     8401 ttgccttatc caatccgcct tcaagaatta gcagttactg ctgatacgat cacaaaaatc
     8461 atgaaagagg tcaattatcg agaagaagta gcaggtgtga tcacttggat gcatactttc
     8521 tcaccagcaa aaatgtggat ccgcgggaca aagcttctac aaaaaccatt gcttcattta
     8581 gctacgcaat ataacgaaag cattccttgg aaaacgatcg atatggattt catgaattta
     8641 aatcaatccg ctcatggtga ccgcgaatat gggttcatca atgctcgatt aaataaacaa
     8701 aataaaatcg tcgttggcta ctggaaacga cctgagatcc aaaaagagat tgctgattgg
     8761 atggatgttg cagtagccta taatgaaagt tttggaataa aagtggctcg ttttggtgac
     8821 aacatgagaa acgtgggtgt cacagaagga gacaaagtgg aagcacagat tcaatttggt
     8881 tggactgttg attattttgg tatcggagat cttgtacaag taatcgatcg cgtatcagac
     8941 gaagaagtcg atcaattatt tgaagagtac aaggaattgt ataccttcga ctatggtgat
     9001 tatgaagaaa agacatggga ggaacatgta aaagtacaag ctcagcagga aatcggtatt
     9061 cgccgtttct tagaagaagg aggctataac gcttttacga ctaattttga agacctgtat
     9121 ggaatgaaac aactacctgg actagctgta caacgtttga tggcagaagg atacggtttt
     9181 gctggggaag gcgactggaa aactgcagcg attgatcggt tgctgaaaat catggctcga
     9241 gggaaagata caggattcat ggaagattat acttacgagc tagctagtgg ccaagaagca
     9301 atcttggaat cccatatgat ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc
     9361 gttgtttctc ctttatcaat gggagataga gaagatccgg cgcgtcttgt atttgatggg
     9421 aaagcaggag aaggggtagt cgtatcgatg gcagatttcg gtacgcatta taaactgctg
     9481 atcaatgaag tagaagcctt tgaaccaacg actgaagcac ctaatttacc tgttgcacgt
     9541 gtgttatgga aaactaaacc gaatttccat gaaggtgtcc attcatggat tcaggcaggt
     9601 ggcggtcatc ataccgttgt ctcgctaaat ctaacaacag atcaaattga aacgtgggca
     9661 aaattagttg agttggaaac agtggttatt agataaaaag aggttgtgaa aaagccactt
     9721 agttttaaga aataagaaga gattgacgaa atttgctttt caactttttt gaaatttagt
     9781 cttattatca atgtcagcta acggaacacc atttatcaag atatctcacc ctcaatattt
     9841 tgctctcgaa aataaggctt atggcagtaa cttgtcatag ccttattttt atcactcagc
     9901 ctactctgct ctaactgctc tttttgcttc taggaagtca aatggtaaac agttgtctaa
     9961 ttgcagaaat ttcgagatac ttgttagtaa ggagggggtt ttatgtatca cgttcatttg
    10021 attgatgcta ttccagaaca agagatagaa aagttacaag gggaatattt caaaataata
    10081 gatgatttgc cagccgaagg aatagttatt cgtagtacga aagtgaaaga tgaatggctt
    10141 acaccagatt tgctagcaat ttcaagagct ggtgttgggg tgaacacgat caacgttgaa
    10201 aaagcttctg aaaacgggac gatcgtcatg aatacaccag gtgtaaatgc taatgcagta
    10261 aaagagcttg ttttgtgctg tttgctctta tcttcccgtc cgataatcga agcaagtcgt
    10321 atggtacaaa cactaacagg tcctaatatt ttagaacagg cagaaaacaa aagaagtgct
    10381 tatgtgggac gagaattgca aggcaaaacc atcggtcttc ttggtcttgg agcgataggc
    10441 acaaaagtag cgctaagttg ctatagctta gggatggatg tcttgggtta ttctatccgc
    10501 gatgcccagt tggattatgt tcggcaagca gatttagaaa cagttctttc tacctcggat
    10561 tacatcgtag tcatgctgcc gttgacagaa gatacaaaag gattgatcga tcaggcgaat
    10621 atagaaaaaa tgaaaaaaga tgctgtactt atcaatgtag gaagaagtga aattgttgat
    10681 aaatatgctg ttatgcaagc attggaaaaa caacatctgg ctaaatactt gactgatttt
    10741 ccagaagaag agttcttaga aaatgaccgt atacttatgt tgcctcatct agggggatcg
    10801 actcaggaag ctttggcaga cagcggacgt ttggcagttg aagccttaaa agattatctt
    10861 ttgttcggta cggttagaga agcggtcaat tatccatccg cacgaatgtt tttccaagcg
    10921 ccgtttcgat tcactatatt ttatcaaaag aaaaccaata tattagcaga gatattttct
    10981 ctgctgaatg agaacgagct agcgatcgca gatatatcgc gtaaccataa gaatgagcat
    11041 gtctacatat tgattgacat agacagtact gattttgaac agctcaatca agtgaaacaa
    11101 cagctagaaa aaatttctgg agtacgaaag gtccgcttat taaagaaacc agaaaggcca
    11161 taaagaaaga gaatatttgg atttcattaa ttcttctttc cgttctttac tttcgaattt
    11221 cgtacaacta aaataagaga agcaacattc ttttattgat agaaatgaag gagcaagcat
    11281 ttgaaaagat atataagcag actttcgacc aaacagaaaa agattttaaa aggaacagca
    11341 gcaatcatca gtatcttttt tcttgttgtt tttagtaatt tatttttgca gtggtgtcaa
    11401 aatgatctat ccgtagacct tgcgttgaaa tttgcttttt cttggcatac ggaaaaattc
    11461 ttcttagcct gtctcgtctt gctgattatt cttatttttc tgattgcact agcaggatct
    11521 gtccctttag gttcccttac gtatgtcgta gccatcggtg tattaggttt tgcaaattat
    11581 atgaaaatga gttatcggca agaaccgatt tatccagacg atttgaagat gatcactgaa
    11641 atcggattgc tgaaagatat gaccggcact atgctgttta cagtgatctt agctgccgca
    11701 ggaaccgtga taggattgtt ttgctggtac atgtttcgaa gcctgaaaaa aggaaggcga
    11761 ttccaattga tccgattaac gacactgctt gtggcaattg gactgctagg gtatatcagt
    11821 aatttcaaca atcccgataa tttgttgcga aaagcatata ataaaacggc actatggata
    11881 ccttacagcc agaaaatgaa ctactataac actggtttta tcggtgggtt tttgtacaat
    11941 ctgaaggtag aaccaatgga tgaacctgaa ggatattcga aagaaaagat caaagaaatc
    12001 actgaaaaat accagaagct agccgatgaa aaaaataaaa ctgtagagga agagtcgccg
    12061 aacatcgtgt tcgtgatgag tgaaagtttc tcagatcata gtcgattaaa cggcgtagaa
    12121 gtatctggtg agccattggc agattattat gaagtagctg atcaaacata tagtggcaat
    12181 atgctttctc aaaactatgg cggtggaaca gcaaatatcg aatttgaagc attgactggt
    12241 ttttctatgg cgttatttaa tgcgcaactc accacccctt acacgatgct tgtaccaaaa
    12301 atggatcagc ttccttctat cgtttcagcg ttaaacgcac aatcatacca gacgacagcg
    12361 atccatccat acaatacttc gatgtataaa agagaggatg tttatcagac attaggattt
    12421 gaccagttca tcagcgagcg tacgatgacc tatacggata cgatagagaa taatccatat
    12481 atttctgatg agtcagctta caaagaagta atgacacttc tcaaggaaga aaaaacacct
    12541 cagttcattc atttagtcac gatgcagaca catatgccct acgatggaaa gtatgataat
    12601 ctagactatc ctgcaaaaac ttcagatgga agtgggacac ttgacttaga gaattatctg
    12661 caagatattt cctatagcag cacggcactg aagcagttca cggaagaatt gaagaatctt
    12721 tctagaagga ctttagttgt tttttggggg gatcatcttc cagggatcta ttcagatacg
    12781 atccaagcaa aaaatgacaa acaaacattg catgaaacac aatttcttat gttcgacagt
    12841 aaaggagaat tagaaaagca aacgactcac gatgcaatca ccagtccgtt ttattttgca
    12901 gcgaatctaa tggaacagac gaatcagaca acaaatggct tttatcagct gcttttatca
    12961 ttagaacagg aacttccggc gtttgaacga gaactgtact atcaaaacgg acagtggtat
    13021 aaagaagcgc aattcaatcg ctcgcaacaa gagatttacg acgaatatca attgatccaa
    13081 tatgacattg tcgcagggaa acaatatagt ttagcagatg gtttctttga gcacgaataa
    13141 gaatggcgag ctgtatagaa accattaaat tttttcaagt acttgtcatt tgaggataaa
    13201 aacaagtaga ataaataata acaacaatgg agaaagtagg aaaacgaaca tgttaccgaa
    13261 tattggctat tatgtgcagt ccatcgatca aatcgtcaag gaaacagaag caatcggaga
    13321 aggaatgcat ccgaaatatg aagtgatcag aaacgcaatt gatgaaggaa aaacaagtga
    13381 actggacaaa tcgactcttg aagagatcat cctgttattt gaagaaggaa caaaaaaata
    13441 tcgggcaatg cttatgaaag tcagtacttt gcgtccacca gcaaaagcaa tgggcattca
    13501 taaaaatttt gaaaaagctt atctttcata tgtagcaggc tgtgaagaaa tgatccaaag
    13561 tatcgatctt gaatcagggg tagatgaaga tctattcaat acatctgaag caaaacaaga
    13621 ccaagcgaca gaccaattga cagctgccat ccaaaaaatg actaatttgt tgttgaaaaa
    13681 ataaaagaaa tctttgataa caataaaaac tagagttatc taatataaat gtaatattac
    13741 ctttatgata gagtgatcaa aatcctttat gataatgaca tacctataac caacaacaac
    13801 cttgtactta ctcaaaatcg agtaaatact ccttttccgt cgagtctaac catctcggcg
    13861 gttttttatt tgtagaaaag aacatcagga tagacggaaa agtcttgtcc tgatgttctt
    13921 ttcagatagt atggaaagct gattagtttt ctacattctg tacatattgt tcaaagtagt
    13981 tctgtaaatc ggctttgagt ttctgatatt cctcttcgct atactcttta tcttcgattt
    14041 tcccatcaaa gaaaggaata tttagttctt cccgtagtct gtctaatacg tcttgctgat
    14101 tcatttcatt ctccttttct tactaacgat caaaataaac acagacacat tctggtacat
    14161 agacatcttt tttcagtaat tccagtaatc ctgtttcagg atgacggcga tataatgtca
    14221 gattatcggt gttctgattg gcacacacga caaaattatt gcttggatct agtgcgaaat
    14281 cgcggggaaa atccccttct gttgagatga tttgcttgtt ttgaatgtgg gaaccatctt
    14341 caaaaactgc gaatacagca atcgagttat gtcctcggtt agaagcataa aggaacttgc
    14401 catcatttga aatgcggata gctgcccctc cattttcacc gtcgaattct tctggcaagg
    14461 tcgatatttt ctgtaattct tcaaaagcac ctgtttcttt attataagaa agcacgcgga
    14521 ctgtgctgtc taattcacca aataaataag cgaattgtcc atttggatga aagacaagat
    14581 ggcgagggcc tgttcctggc tctgcagtga aggtagttac aagattcaat ttgccgtttt
    14641 cggacacatc ataagtatac acacgatctg taccaagatc acatacgact aatcgttggt
    14701 caggagtcag atccgtatag tggacatgcg catgatcttg gtttttatga ggacctgtcg
    14761 cttcttgatg gaatattgaa tctgctgctt tcagttcgcc atctcctaat atatggtaga
    14821 catttacttc acctttatga tagttcgccc cgtatagtaa ttgacgtgct tcatcaaccg
    14881 ctacataaca caaaggagcg ccagactctg ttaccgcatt caaaaaatga aaatccttat
    14941 cgtacgcacc ggcaccacct aatccatcca cactagtaac tgtgtaaaga ttgccagctt
    15001 tactttttgc tacataggta gggctatttt cttcaacagc taatgaaagg ttggctaatt
    15061 cttctttttc tgtatcaagg tcgatcgtat agattccttt actttcgcga cgcgtatagg
    15121 ttcctaaaat tatcttttcg atcatatgag ctgattcctc ctaattgtaa tgaaatagtt
    15181 ttcgttttta ttctatcaca taattattcc acacggctaa agaatgagaa tgtgttatac
    15241 tgtttttgaa agcaattaaa ggagagagtg caatgattca ggcaaagatt acagaaattg
    15301 ggacagaagc aataaacaag gaagaaccca tgctgatttt gttcgataaa acagcaacag
    15361 ctgccttacg aaaatattcg gtgattcaag aagtatcaaa agacgaacct ttttctttga
    15421 aagcaggcgg aacaattgct tttgatgatc aggtatatac gatcgattat gtcggtccga
    15481 tggccaatgg gaatttaaag acagtagctc acgtatcatt gatttttgat gcttgtccag
    15541 aagaaggaca aattgcaaat ggaatctatc ttcatccgca tagactgcct gctattggtt
    15601 tgggaagcca aattttttat aactaacagg aggaattatg gaaggaaaaa ataagaagaa
    15661 cagaccggta tcgtggtttt ggcgctggtt tttaaataat caagtagtca cagccttact
    15721 tgttgtattg ttaatattgc tgatcgtctt tttatttaca aaagtttcat atttgtttgc
    15781 accaatctgg caatttttag caatcgtagg tctgcctatc attttagcag gaatcttgta
    15841 ttacttgatg aatccggtag ttgattactt tgaaaagcga aaagtaccaa ggctttacag
    15901 tatcattggg ctgtttgttt tggttgttgc actaatcgca tgggggagcg tagtgatcat
    15961 ccctaagatt caggaacaaa cagtgagctt catcagtaac ttccctcaat atgtagatac
    16021 gattgataat aaattaaccg agattttgcg ggacccgctg ttcaaccaat ttcgcgaaca
    16081 gttggaaaca gcaggtgata agttcatgaa ttcggcaggc gatatgatcc aagatatatc
    16141 taagagtaca gttcagagtc ttggaagttt tgtcggcgcg gtagcgacta ttttagtggc
    16201 tctgctgacg atgccgttta tcttatttta tctattgaaa gacggtaaac aattagcacc
    16261 ttattttgtc aaatttttac cgacacgcat gcaaaaaccg acgttgaatg tgctgagtga
    16321 aatgaatagt caagtttctt cttatataag aggacaacta actgtggctt ttgctgtggc
    16381 aatcatgttt atgattggtt ttgcggtcat tggtttagag tatgctgtca cgttggggat
    16441 tattgcgggt tttttaaacc tgatccctta cttaggatca tttttagcga tgattccggc
    16501 tatcttttta gccattgtag caggtccatt catgatcatc aaagtactga tcgtttttgc
    16561 agtagagcag acaatagaag gacgcttgat ctctccatta gtactaggga atcagttagc
    16621 aatccatcca gtaacgattt tattagttct gctgacttct gggaaattgt tcggaatcat
    16681 cggtgtgatc ttggggattc ctgtttatgc agcagctaaa gtagtgatta ctcatgtttt
    16741 tgaatggtac acaacaatct cttctcttta tgaagaaaat ccgttgaaaa agcagaaaga
    16801 ataacaaaaa gagacagatg ggaaaatatc caaggcacaa atgctgcttt gaagattttt
    16861 tcctctgtct cttttgtatg catttatctc aatttttaaa atgatttacc taaagcagca
    16921 gctttatcta atgcattttg catcaactca tctgcacggt cagggtcata atcgattcct
    16981 tcgatgaata gctgatctac ttgatcgatg cctacaaagt ttaaaatacc ttttacatat
    17041 tgagaggcaa agtcttgtcc gttgtataca ccgccattag attggatgtg cagtgctttt
    17101 ttacctgtca ctaatccttc tggcccgttt tctgtatagc ggaacgtttt tccagctaca
    17161 ttgacggtat cgaaccaagc ttttaatttt gttgggatat ttagattcca taaggcgttt
    17221 gcgataacga ttttatcagc agcaaggaac tgatcagtca attcattgaa acgagcaatt
    17281 ttttgttgtt gactttctga taaggaagtg aattctgcac cagcacgtaa tgcttcccaa
    17341 cctgataata attcttcatc gatttccgga atatcatcgc gataaagttc caaaacagtc
    17401 acttcatcat ctggatgatt ttctttataa cttgttaaaa aagacgtcaa tgccttaact
    17461 gtgcgcgatt cttctgcagt taggggatga cctttgacta ctaaaacaga tgccatatgc
    17521 ttaccttctc cttttattgc tatgattgtt ataactatga ctaacaaaat cagtttactc
    17581 gaaaaagaaa aggctatcaa gtcatattga taaggttgaa gaataaataa ctaaaaaaag
    17641 acagggtata aaagaggctg tgacacgatt ttttgtcaca gcctcttttg aatctaacaa
    17701 gcaatattca agcaacactc gactgaagag cttccatccc accaatgata taaacagcac
    17761 gaaaaccatt agcagtaagt tgatcggcta atgcttctga tcgcctgcct gtatagctta
    17821 gaatataata aagtttcttt ggatctagat tttttaattg tcttggcaag atagcagcgg
    17881 ggatagaaag cgagtggctt aagtggtttt ttgtgtaatc taaggtattt cttgtatcta
    17941 aaacgatgct gtcagcatcg agaggcaatt tattgaagtc ttggacacta attgattggg
    18001 ccatttatct catcctttgt ttagtatgtg gctattttaa tgggcaactc tcattaattc
    18061 aaatggaagg attcctctta tcactttttt aatgtatcgg atgataatga aaaaaactat
    18121 gcaaatgtct tgcaaaacag gtaggaggtg ttataatgac atagcgtcat atgacaatgt
    18181 gtcatcttga aagaaaggag aatccatatg ccgacacaga catttttcca tttgcctaaa
    18241 gaaaaacaaa aaagactaat cgaggctgcg agaatcgagt tttctagagt acccttaaaa
    18301 gaagcttcga ttgctaatat cgtcaaatta gcggatatcc ctagaggcag tttctatcag
    18361 tattttgaag ataaagagga tctttatttt tattactttg agacaatgag acgtgacagc
    18421 tctaaagata tgttacaact aatgaaagaa tccgatggtg atctattcaa aggattcgaa
    18481 tcctattttt ctaaaatgat ttatgagatt ttggaaggcg agaatgcttc tttttatcgt
    18541 aacttgttta tgaacatgga ttaccgtgcc tcaagaaaag tcgctcctca tacgagtcgc
    18601 caaaaaacgc ctcacgatcc acataaagaa cacaaacaag atgcaaaaga gttgcttgaa
    18661 ttgatcgatc ggacgaagct gaaagtaaaa aatgatcatg aattggaatt actgatccaa
    18721 ttagtgatga atactgtatt tacaacaatt gcgcattcgt atcgcttatt ttcaacatct
    18781 gaagattact cagtagagca agcaatcagt gaattcaatt tgaaactttc ttggctgaaa
    18841 aacggagtat ataaatagaa agaagggaac tagatgctaa aaatcgtaaa acggatctcc
    18901 ttgacttcag caattgcagc tgcagttttc atggtgatcc aagtactggc agatttatat
    18961 ttgccgacac ttacctccaa tattattgat aaaggggtag cgcaaggaga tgtcgattat
    19021 atttggcata caggttttgt gatgatcggc ttttctttga tcagtatctt agctgctatc
    19081 gcaaatacat tttttgctac tcgtgaatca cagaaactgg gtaagcaatt gcgtacggat
    19141 gtctataaaa aatcagagag tctgacaaaa gatgcgtttg ataaatatgg aacggcctct
    19201 ttgatcacaa gaacgaccaa tgatgtgact caaatccaaa tggttacaca aatgttttta
    19261 cgtatgatga tcaatgcacc aatcacgttg attggcgcga gcatacttgc ttatcaaaaa
    19321 gatcatcagt tgacaaaaat cttcttagtt gtcatcccag taatgattat tttgatcggc
    19381 ggaatcatgt actttgctgt tccattattc aaaagtatgc aaaagaaaac tgaccgttta
    19441 aacctggtat ttcgagaagg attgacaggg gtcagagtga ttcgagcatt cgataagaca
    19501 cgctttgaag aaaatcgttt tgatttagcg aataaagatt atacaaatac ggcaatcaaa
    19561 gttaatacga tcgtcgcatt gatgatgcca atgatgacat tgatcatgag cggtacaaac
    19621 gtagcaatca cttggtttgg tggacactac atagcagata tgactttgga agtcggtaat
    19681 ctgattgcat ttatgactta tgcgatgcag atcttgatca gctttatgat gctttccgct
    19741 atttttatca tggtgcctcg tgcacaagct tcagctgacc gtattaatga agtgttggat
    19801 gaaaaaatcg gtatccatga ccctgaaaat cctaaaacag tttcttttgc tggaaaaaac
    19861 gcaacattgg ccttcaatca tgtcaattat cgatatcatg gagcagaaaa acttgcattg
    19921 gaagatattg atttccaagc caagtcgggc gagatcgtcg caatcatagg ggggactggt
    19981 tcaggtaaaa cgaccttagt caatttgatc cctcgattat atgacatcga atctggttcg
    20041 atccaaataa atggaacgga cattagtgac atgactcaat ataatttgcg agaattaatg
    20101 ggctttgttc ctcaaaaagc agttctgttc tctggaacga tccgagacaa tatgcagtat
    20161 ggtaagccgg atgcaacaga tgaaatgatt tggaaagcat tagaaatcgc acaagctaaa
    20221 gattttgttt cagagatgga agatggctta gatagccatg tggaacaagg aggaggaaac
    20281 ttctctggtg gacaaagaca gcgtctggca attgcacggg cattagtcaa aacagcagat
    20341 atctatgtgt ttgatgattc cttctcagcg ttggatttca aaacggatgc caatcttcgc
    20401 caagcattga aaacgaatat gaaagaaagt atcaccgttt tagttgctca acgagtaagt
    20461 acagtaatgg atgcagatat gatccttgtg ctagatgaag gaaaactggt tggcaaagga
    20521 acacatgaag aactattagc aacaaacgaa acttatcaag aaatcgttca ttcacaacta
    20581 agagaggagg acttagcatg agtaaaacaa acacacgtcc ctctcatgga ccagcaggcg
    20641 gtccaggaca tggtggacct ggcagaaatc tcggtgtaaa aggtgaaaag cctaaaaatt
    20701 tctggggaac ggtacgccga ttgtttggct atatgtctaa acgtatggtt gcgatcatcg
    20761 ctgtatttgt actagcaata gcagccgtga tcttccaaat ccaaacacca aaggtcttgg
    20821 ggaaagcaac cacagaaata ttcaaaggtg taatggctgg cgctcaacag atgcaacaag
    20881 gccaacaagt aacaaaattt ccgatagact ttgataaaat cgcgcagatc attgcgacag
    20941 taatcgtgat gtatttgatt tctgctgtat tcaacttttt acagcaattt attatgacac
    21001 gtgtttccca acgaacagtt tatgaattac gtcatgattt ggaagccaaa atgaatcgtg
    21061 taccgatttc ctattatgat acacatacaa atggggatat catgtctcga gcaatcaatg
    21121 atatggataa catcgcaagc acgctgcagc aaaatttgac tcaatttgta actagtgcag
    21181 tcacattgat tggggtcatc gtgatgatgc tttcaatcag ctggcaattg acgttagtcg
    21241 cgttattgat gatcccttta agcttgatca tcgtgatgat cgtagcgccg aaatcacaag
    21301 tgtttttcgc agatcagcaa aaaagtttag gtttattgaa taaccaagta gaagaaacat
    21361 atggtggtca taccattgtt aaaacattca atcatactga aaaagaccag gaagtatttg
    21421 aaaaagaaaa tcagaatctg tatgccgctg gttggaaagc tcaatttatt tcggcaatca
    21481 tcatgccgtt gatgaatttc gttaaaaatc taggctatgt gttcgtcgct gtactaggcg
    21541 gagtaaaagt tgcaaatggg aacatgacat taggggatgt tcaagcgttc ttgcaatata
    21601 caacacagtt ctctcaacca atcactcaac ttgcaagttt gatgaacact atccaatcaa
    21661 cagttgcttc agccgaacgg gtatttgaag tgttggatga agaagagatg tccaatgaaa
    21721 aatctggcct tccagaagaa aaagatacac catacaaagt tcgttttgaa aatgttcaat
    21781 tcggctacac accagataac ttgctaatga ctgattttaa cttggatgtc aaacctggtg
    21841 agatggtcgc tattgtaggt ccgactggtg cagggaagac gacactgatc aaccttcttg
    21901 aacggttcta cgatgtttct ggaggtagta ttcgttacga tggtgtagat acccgtgatc
    21961 tttcacgtga tgaattgcgc gcacagtttt caatggtact gcaagatact tggctgttta
    22021 caggaagtat ctatgacaat atcaaatatg ggaacgacga tgcaacagac gaacaaatca
    22081 ttgaggctgc taaagctgct cacgtagacg attttgttcg taaattgcca gaaggatata
    22141 acactgtatt aaacgaggat gcaagcaata tttcgcaagg gcagcgtcag ctaatcacaa
    22201 ttgcgcgggc attcttagca aatccagatg tcctgatcct tgatgaagca acatctagtg
    22261 tagatacacg aacagaaata ctgattcaaa aagcgatgaa ccgcctattg gaaaaccgga
    22321 caagtttcgt tgttgctcac cgcttgtcta caatacgcga tgcagacaac attattgtga
    22381 tgaatcatgg ttcgatcgta gaaacaggaa accacgatac attgatggaa aaagacggat
    22441 tctatgcaga tctttataac agtcagtttt ctgaagaaga agcttcatag aatagtacaa
    22501 atttattatt ttttttaggg gctgtgacaa aagtcgtgtc acagtccctt tttccgaata
    22561 aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgagaagc
    22621 tatttttccg ttttgtatct ctaatactca ggtcaaaccg ctgattttac aacctcttcc
    22681 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcaa caaacctgag
    22741 aagctgtttt ttgaaattgc tccttgtgac tcggagttaa acggcaggct cacaacctct
    22801 tggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
    22861 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct
    22921 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    22981 taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca
    23041 acctcttttt ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt
    23101 tctcacattt tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta
    23161 aaataaagct tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc
    23221 acctatttaa aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac
    23281 aagaacgttg ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca
    23341 gaagaagctg gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg
    23401 ggggtgatca aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca
    23461 gatatccttt gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat
    23521 ggacgtatgg ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa
    23581 gagaataaga acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga
    23641 aaatctgaag aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta
    23701 aatgttacta acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta
    23761 cataatgaag aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca
    23821 gaagctagcg gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg
    23881 aaattcatga tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca
    23941 gttgatttct tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc
    24001 gaatggtggt tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct
    24061 taactttttc tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat
    24121 cgcttattct agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg
    24181 cgcagttttt ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg
    24241 taagaatctg atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt
    24301 gaaaaagatg gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca
    24361 agtagaaaca tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga
    24421 agatctgttg tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt
    24481 acgtctatta gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga
    24541 atttgaaaat cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac
    24601 atggatacca aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct
    24661 agaaccaggt gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct
    24721 tgggttgatg ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt
    24781 attggaacca ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt
    24841 tcccacagaa caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc
    24901 aagagttttg gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat
    24961 tacttttgtc caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga
    25021 agagacgatt gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt
    25081 aggatgggac ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta
    25141 tacaggtacg tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa
    25201 tcgcgtacat cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga
    25261 aaactttttg aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg
    25321 agaggagttt gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt
    25381 tggggaggaa gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga
    25441 gaagattggg cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc
    25501 aaagggaaaa gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca
    25561 gaaccagtgt ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa
    25621 gtacatccag atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc
    25681 tggtatatca tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca
    25741 agagaagaac tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta
    25801 ccagttaaaa aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa
    25861 ggaatcatga ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac
    25921 gacagaaaag atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta
    25981 acaactgtcc ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca
    26041 tcgatcgtca cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga
    26101 atcacatctt tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga
    26161 gaccttgtcg ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca
    26221 aatggcgttt ctgagtgggc ggtccaaggt gaactagcaa tcattgcgtc tgaaccaggc
    26281 aaatagttcc cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat
    26341 aaaatccgtc catcaataaa tagaagtctg gtgaaaaaga tggaatacaa atggaaagca
    26401 aacaacagca gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa
    26461 atgatcatgc caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa
    26521 ggtgtgtctg gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg
    26581 cttttaccca tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca
    26641 gtcgccggtg caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta
    26701 ggagcgatga ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc
    26761 catgtgaaat tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact
    26821 ggtagcgtta tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac
    26881 ttgttgctcg cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt
    26941 ctgatcttag aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg
    27001 acacctttag gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag
    27061 gcaaatcctg gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag
    27121 gaaaaagtaa atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc
    27181 tattttccat ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca
    27241 agcggcacgt tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg
    27301 tcactactcg tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg
    27361 atagcaatca gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat
    27421 aaagaaaaag agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt
    27481 cattccgtca tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg
    27541 ctgaaaaaga aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat
    27601 gaattgaata accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca
    27661 agagaaaaag tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc
    27721 ttgctgtcac gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata
    27781 gcagatatca cagaacaaaa aacgtcgcct gctaatattg tatttttgta taaaaacaac
    27841 agacgcggct cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa
    27901 aaacagtttg ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt
    27961 tatatcgcac aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta
    28021 gttctagttg aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata
    28081 taagatgtat ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc
    28141 acctgtatct atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg
    28201 cgaattagat aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc
    28261 tagaggctca tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac
    28321 agatgggaaa agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt
    28381 gttactgacg acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa
    28441 taccactttt tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga
    28501 aatcattcgg aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac
    28561 agcgaatgta ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga
    28621 tgcatcactg aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa
    28681 agtaatcggg gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca
    28741 tttggttttg atgctgacat tgacgatgga gcgtgtgacg aaaaatcatt tactgacaaa
    28801 tgagacatat aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc
    28861 acggatcgct tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg
    28921 attattaaat gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac
    28981 acagcttgct tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca
    29041 tttttatgaa gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc
    29101 aagagccgtt ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca
    29161 atacgaggaa gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa
    29221 attatccgaa gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag
    29281 tccgaaagaa agcaatatca gtattgcagg attttcgcca agtggattag cttctactag
    29341 catgttggaa ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg
    29401 gattgctgat ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc
    29461 tattttacca ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga
    29521 agtcaagcag ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac
    29581 gtataacagc aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat
    29641 ggatcaaatc aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac
    29701 gatttccgaa acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga
    29761 agaggtggtt catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca
    29821 tacaaatatg gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat
    29881 tttcaatttg aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc
    29941 ccgtatgtta gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg
    30001 aaagatcagt ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa
    30061 taaagaaatc gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag
    30121 gtgaaaagat gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt
    30181 ggaaagaagc gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg
    30241 catatatcca atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg
    30301 tagccttgcc acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag
    30361 tccaagtgcc tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct
    30421 tatttgtagt ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt
    30481 tctgttcaga catcgaccaa gtaatggcgc tcagtgatgc ggaaaccatt gaagaagtcc
    30541 aaatgatttt tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc
    30601 atcaagtaat aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct
    30661 tattactgcg gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat
    30721 aggggacggt gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt
    30781 ttttatatag atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt
    30841 caagatacca ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc
    30901 tatgatcaca ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat
    30961 ccatccaatc gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa
    31021 aagattgact agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa
    31081 gagcacccac aacaacagga acagaaagct ctggaaaaga tactcataca tcggcaggat
    31141 tcgtatctcc tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt
    31201 gattattact agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc
    31261 ataataaatg gtaaaacgga tgctatgttt ctttaccata ctatccggta atagattgac
    31321 tagagagatt acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt
    31381 tatacataaa atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc
    31441 taaagagaga tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat
    31501 ataacggagc aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc
    31561 agaaatcagt cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg
    31621 gacattccct gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa
    31681 actttccaca aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat
    31741 ctcttcatag ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa
    31801 aagtgggaat gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt
    31861 tagtaagaga ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga
    31921 taaatagcta aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga
    31981 aaacatgctt tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc
    32041 gtcttcccct ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg
    32101 tctaatccgt tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca
    32161 gcaagcaaga catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac
    32221 tcagtcatct tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat
    32281 ttgatcaaag tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct
    32341 agaaaaaaga aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac
    32401 aattgatcat aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca
    32461 aatccgttcg gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt
    32521 cgaatcacac gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat
    32581 aggcctccaa tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta
    32641 acaaaagtta ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga
    32701 agttttctga taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt
    32761 ttcgtgccaa agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa
    32821 ggagagaggg agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc
    32881 gagggtacaa aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact
    32941 gattgcctgg ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc
    33001 tttcgctacc atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac
    33061 tggcggaaaa gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg
    33121 tgtgattgtc ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg
    33181 tggatatgcc atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga
    33241 aatgctagtc aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt
    33301 ttctgcgatt ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat
    33361 cattttatct gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt
    33421 atttttgaat aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc
    33481 agaggctgga aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt
    33541 attacttgct tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat
    33601 gatcattcac tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc
    33661 gttattattc ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat
    33721 ggatgctgga ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac
    33781 accgttcaat tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt
    33841 tggtatttct gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa
    33901 atctgttcaa gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca
    33961 aggagctaca ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc
    34021 aagtgcgatg ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac
    34081 ggtgaccaat caagcaatca gccaactgac agatgactcg aatacattga tcgttactca
    34141 ggtcgaacta caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga
    34201 aaactttttg aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa
    34261 aacatcagtt tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa
    34321 acaaatcaaa gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat
    34381 gaccgtgctc aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa
    34441 tatccaagat ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga
    34501 aaaagtacag atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc
    34561 gcaaaaatac gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc
    34621 atgcaaagtt tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa
    34681 gcgatccgct attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc
    34741 gatgccatgg tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt
    34801 cctcatggaa cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa
    34861 gtaccagatg gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc
    34921 ggtattgcag gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc
    34981 agtgatatgg acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa
    35041 aatctagcaa ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat
    35101 atcggtcgag gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta
    35161 gatgtgaatc acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta
    35221 gcagatgaat caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca
    35281 aatccagaaa aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc
    35341 ccgaatatct taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag
    35401 gaaggaagca caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca
    35461 tttctagaaa aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc
    35521 ggctttccaa atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta
    35581 ttcgtccaag tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa
    35641 aaaatccaat taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa
    35701 ctatttagtg tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac
    35761 cagaccattg atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg
    35821 caggaaacag gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg
    35881 tatattgaaa aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt
    35941 gtcgctcgga caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga
    36001 gaactacaag aacataactt gacttgccca catttgacgg caacaatggg gattattttt
    36061 aattactatg atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa
    36121 aacttggaac aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat
    36181 atcaagcaaa acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg
    36241 ggaatgtgac gaaatttttg tcacattccc ctttttcgaa taactaggct gtttgtcctt
    36301 caagataggg cagatccaat tctttaccgt aacgttgttc taaagctagg cggatcaaac
    36361 gagctgccag gttctcattt cttgcaagga taggtccatg gaaataagaa ccgaagacat
    36421 ttcggtaaat gacaccttca gattggtctt caccattatt tccttgacct tgtaccactt
    36481 tccccaaagg tcgttcacct tcacctaaga aagtccgacc attgtggttt tcaaatccat
    36541 aataagtttc attgaattct tcgttatgga taacgatatc accgataaac cggttatttt
    36601 cctgacttaa cgtgtagtga tccagtgcac tgattccttg gatcttttct ccattagctc
    36661 ccatgtagta gtgacctagc aattggtagc caccacaaat ggctaacatg acgccatcat
    36721 tttcgatata gttgatcaaa gaatctttct ttttctggat gtcttttgaa atgattaatt
    36781 gctcaaagtc ttgaccaccg ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt
    36841 catgtatact aacgatctcc gtatggcaag aaacccccat tttttctgct atatatttca
    36901 acatcaaaag gtttccgtta tcaccatatg tgttcatcaa atttccataa aggtgggcga
    36961 tattgagttc ataattagcc acggttcatt cctcctttga taaatccttg agcagctagt
    37021 tctttacgaa gctgcaatac agcagtgtaa gtagctaaaa tatagacatg ctcagttggc
    37081 aattctttga ttttttcgat tacttctggt aattcttttg tttctgtcaa tttattttct
    37141 ggaatcccgg caacttttag acgtaaagtc atatctgtat gacggtctcc tccagaaata
    37201 acagctggga tatccatatc ggcaaagttt tcaaaattac catcccaaat ccagctgaca
    37261 tcgattccgt ctgcgtaatt ggcatttaat aaagccacta aagaaaatgg ataaggagct
    37321 agaccaatca tatcgattac ttgattgata cctacaggat ttttgacaag tactaatgta
    37381 cattttttcc cattgatatt gatgacttct tgtcggccga atactttctc gtcatatcct
    37441 aaaccttgtt tgattttatc aggatcgaca ttgaagtact ctgccactgc agtagctgct
    37501 aaagcattgt agacattata cataccgcca acttcgattc cgtattcatg accatcgatc
    37561 acgaaatctg ctgacacatt gtccatccgt accatatcag ttaatcgata atccaattca
    37621 ggacgtttga agtcacaatt tggacagtaa tatttcccta agtttgcata agtgatcatt
    37681 ttataatgca agatgtgatg acaatttgga cataagacac cgtctgtatt gtaatgagcc
    37741 atttgttcac gatcttcttc atggtcaaag ccataatatt ttctaggatt gactgtttcg
    37801 atagaattga agattggtga atcaccgtta ctgataatcg ttgcatttgg tgactttgcg
    37861 gcaccgtcta caatcatttt ataggtcgta tagatttctc cataccgatc catttgatca
    37921 cggaaaatat ttgtaaataa gaacagttct ggtttcatat attctgtgac tttgctcaaa
    37981 cttgcttcat ctatttctag gacagcaaac tttttctgtc ctttttttgg ttttgcctgc
    38041 aagaaagtcg atacaattcc ctgtaccata ttcgcaccag tagggttggt tagaacttca
    38101 tcgaattgct ggcgcaaaat attgactgtt aaagcggtag tcaatgtttt accgtttgtt
    38161 ccggtgacaa cgacgacttg gtagtctttt gccaaactat ctagtatatg tggatcgact
    38221 ttcagcgcta gtttacctgg taaacttgat ccgcctttaa aaaaagtttt taatgcccat
    38281 tgcgacgttt ttccaactgc aatcgcaaaa tgactgcgta ttcccattat gtaacctcca
    38341 aaaacgattt gctgacgaat aaattcaact aatcatacca tatgttgaaa aaaaggttaa
    38401 attgcttttc ttattcttaa gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat
    38461 cgcatgattt aacaaaaaaa cagcttgaaa caaaatgatt gtttcaagct gctgtgattt
    38521 tattattccc tcttacctat attcccattt taaccgataa taattttctc gcttggatat
    38581 tcatagccta gatctcgtgt ttcttttggc acgaataaca tcaatgtata tagcattccg
    38641 atccgcccaa taaacatcag tgtagcaatc gtgattttgc ccacaacaga caagtctccg
    38701 gtaatcccta aagataaacc agttgttcca aacgcagaag ttacttcgat gatgatcgag
    38761 atcagcgttt gttcttctgt agcagataaa aaaacgatac aaaagaaaca cataccaaga
    38821 gaaagcataa agacaacgac tgattttcgc acatcatctt gatcgattct ccgtccaaaa
    38881 atattgatgt tatcttcact tttgaggaag gaatatagat acaaaccgat gattgctaca
    38941 gtagttgttc gaatcccgcc gcctacagaa ctaggactgc atccgataaa catcagtaaa
    39001 gaaaaaatga tcagtgttgt gatttgaaaa tcacccaaat cgtgaatctg cagaccagca
    39061 ttacgagtcg taatcgaata aaacatcgaa ttgatccatt tgacactcat attggaatct
    39121 tggaataaat ggtccttttc caatagataa atcaagacag ttcccgaaac aaacaagata
    39181 acaaaagcta acacagctaa tttggtaaac aaggaaaaac gaaacggaag cttcgcattg
    39241 gaacgtttgt ataatagcca ttcccggcac tccatcaata caggaaaacc aattcctccg
    39301 acaaaaatca aaaacatgat caggatcaag aaaaaatagt catgtgcaaa gggcttgatg
    39361 gaatccccag tgacgtcaaa cccagagttc gtgacagccg atatcgcctg ataaaaaccg
    39421 taaaaaacgg catcacgcca acgatcaaaa tagcctctgt aataaaaata aatagaaaag
    39481 aatgtcccaa aaagtaattg aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg
    39541 acgataccag acaaacgcgg ttgattcata tccgtcatga tcagctgtcg ctgtttcagt
    39601 gtgatccgtc gtttagaaaa aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca
    39661 ccaacttgaa ataatatttc cagcagtata ataccgttat cattgaacac agaattgata
    39721 tcaaaagtcg atagtccagt cacactcact gtactgattg ccatgaacag catgtccaca
    39781 aaagaaacat ggctatctgg ttcgcgaaaa atcggcatat aaaatagaac gagtgataag
    39841 accgtcatca atatataata aaaaacgatg atttggatcg atgagaaatg attggcagca
    39901 aacttctgtc ctcgccgctg cagccttcga aagaataaat caacatgcac agtactatcc
    39961 tcctgacaat agtcgtttct accagtatac aaggggaatt aggaatactc aaagaaatta
    40021 tcaaacagat aacaatacag ctttcgatta aaatagaaat ggcttataat aaggcaaatc
    40081 ctagaaaaaa gaaggaggag gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa
    40141 atgaaaaatc aaaaaataaa ttatgataca gttctgaaaa aaatgatcat gcaatgggag
    40201 aaagaagaag taaagcctaa gatattaata catacatgct gtgctccttg cagtacgcac
    40261 acattggaat tcatgtctca atatgcagag atcacgttat ttttttcaaa ttcgaatatt
    40321 catcctaaaa gtgagtattt gcgaagatta catgaacaaa aacgatttgt agaccaattt
    40381 aatgagaaaa ctgaccatca tgttgcgcta atcgttgatg aataccgtcc aaatgaattc
    40441 accaaaatgg ttttaacaaa ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt
    40501 gcatgcttca atatgcgatt agatcttgtt gcgcaaaaag cgcaagaact aggatacgat
    40561 tactttggaa gtgccctgac catctcgcca aagaaaaatg cagctttgat caatcagatc
    40621 ggaatggata tccaaaagat ttacgggaca caatacttgc caagtgattt caagaaaaac
    40681 aaaggctatg aacgctcttt gcaaatgtgc aaagagtatg atatttatcg ccaatgttat
    40741 tgtggttgcg tgttcgctgc caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa
    40801 gcaaaagctt acttaaaaac atcaaccgta gtttttgagg aaaattaaag aaatctttcg
    40861 gaaaaacgta agaaaaaaat aaagaattta tggtagaaat aaggtagact gtataatttg
    40921 tccgtagtaa agtaaggaaa caagaataaa tttcaatgaa ggaagagata agatgcgaaa
    40981 agtagcacct attttgaagc caatcgctgt attaacaatc tcgacgattg tcgcgactac
    41041 tacaggtatt gtgaatagtt ccagctaccg tgcaccaagt tcaagttcta gtactgtaga
    41101 agaaacaaca ccaactactt cagtctcgac atctgaacca acaacaacgg aaagtttgaa
    41161 tgaaaccact acgacaagta catgggacga tacaacaact agttcgtcgt atgatgagac
    41221 atatacaact gacagctcaa gcgaaacaag taatacggta ccagatggca ctccatcgga
    41281 ttcaaccaat ccaagtacaa gtccggatac aagtgctagc tccgaaacga taccaagcag
    41341 tacgactgta gatgataaca cgcaaaatga tgatgcagca cgtacagcac agcaaaacca
    41401 agattcagga aatacccaaa cgtataacca gcaagcaggt gattctcaat gacattttta
    41461 ctagacctta tcaacggggt gcataaattc cttggctatt tagacatcag tcctaaatat
    41521 cttaaccgtg gatatacgat tttaagtgtg attcccacgc tttatttatt gagaattgtt
    41581 tatggacttt ggcaaaacca aaactatctt caatttttct tatatggact tgcatttcta
    41641 gtgcttgttt attttactgt tttaaatgta ttttattact ttttagataa aaacacgaaa
    41701 gccgatgtca ctcagctttt tgtgaagtat ttacctgatg aagcgttcaa catccagaca
    41761 gaagaatcga tagataatgg aaattcgatc gataaagtga atacacaaga ggtcatggtt
    41821 tcttatgacg aagattatca attgaaatta gccgagaata tgcggtattt gattggaaat
    41881 ggtgagatca aaacaaatga actcggtcgc atggatggat ttctggtcga tagaaataca
    41941 ttgtatcctt attactatgt caagaaaaca ggagagaaag agtatgttct aaatattgga
    42001 cgaagctact cggacatgga aaaagtaggg accattcgac ttgactcaac agaagacaca
    42061 ttggaacctg tcggattgtt catcgtcggc ggtgattttg tcaaagacgg gttacgctat
    42121 catgagcctt atcgtctgaa attgattgca aaaaaggcac agtcgcagga agaaccagtg
    42181 gtttatagtc gtagccaaag aagaagacat tcataatgtg atgaaacgcc gtgtatcagt
    42241 ggttgcgata aaaacgttta ttcggaataa gggggatgtg acacgtcttt tgtcacgttc
    42301 cttttttata taacgagaaa gataagacga acacatcact tattctttcc tttcatttat
    42361 aaacattgta gaataaagaa agtacaacga agtattgtga aaaaacgtaa taaataacga
    42421 ggaaaataca agtaggaggt aagctgtgaa agaatttttg aaaaattggg ggattttgat
    42481 ccttgtatta gcggctattt tattagctag agtgtacgtg tttacaccag ttactgtaaa
    42541 cgggcattcg atggacccta cattaagtga tggacaacga ttgatttcat ctaagatctc
    42601 aaactatgaa cgaatggata ttattactac aaaggaaccg ggggatgaag aacggatgat
    42661 tgttaaacgg atcatcggta tgcctggaga tacagtcaag atggaaaatg accagttgac
    42721 cattaatggc aaaaagtatg atgaacctta tctggatgag ttcaaaaaag aattttcaga
    42781 agataagtta caaggagaat atgcttatag ctcaggattc caagcacaag cagaaagttc
    42841 atccaccttt acgagtgact ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg
    42901 cgataaccgc ttgatttcaa aagacagccg aatgtttggg ttagtcgaca aagatatgat
    42961 ccaaggaaaa gttgttttcc gttattggcc tctttcagaa attaaaatca tgtgatgaaa
    43021 aaataaaaag aagctgtgac atgttttttg tcacagcttc tttttccgaa aattccttct
    43081 tatttcttgg agttgctcac tttttttgca atctcatttt aagctttttg gctgatggat
    43141 aagaaaagcg aagcgttttt gttcataaat agcttttccc cacgatggaa ggtatgacta
    43201 gtagcagaga caaagcccgt tttttccaat cgattattca attcttcttg gctaaagcca
    43261 ttatggactt tcggatgact aatttgttca ttcttgtcaa agtctacgat gatcaattga
    43321 ccgccaggag ctaaaatttc ataaagtttt gtaagaatat tctccgtatc aggaatgtgt
    43381 aaaagtacaa gtgatagaag gatgagatta gcttggacag caggaaattc gacagaagca
    43441 tcagcgtgga tcgttcggac atttttcaga tctgctgctt ggatcttttc ttctgccatt
    43501 ttcagcatag tttctgaagc atcggcaatg atcagttctt tgaatctttc cgcaagcggt
    43561 aaactaacaa gcccagttcc gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc
    43621 ggtagataag tagttagttc ttcgcgaata atcttggcta atgcttgacg gtcaggggaa
    43681 tcataatggt tagcgatgga atcaaaaata ttttttgtca tcaaatagct ccttacgtaa
    43741 aactttgaaa ttttaactta ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat
    43801 tcaaaaaggg agaatctaga aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa
    43861 aaggaaagtg tgtccgagaa atattactag acttgcctga gtggtttggg cttcctgaat
    43921 caacggaaaa atatatcgag gaatcaagta aactaccttt atggtgtgaa aaaagaaaag
    43981 aagaatattt aggctttatc actctttcac agacaagtga agatactgcc gaaatctatt
    44041 ctattgtatg ggaataaaaa aagactatca tcaccaagga ttaggaacaa aactttttaa
    44101 agaagcagaa ggttacgcag caaaacatta caaatatttg caagtgaaga ctgtagacga
    44161 ggggcactac tctatatatg atcaaactat ttgtttctac gaatcattag gattttcaag
    44221 gttggaagtt tttccaaatt tatgggatga atggaaccca tgtttagttc ttgtcaaaaa
    44281 attggaacaa aagtgagcaa tagatttaat actaatctgt gcttgatgaa aattctcact
    44341 gtaaaaaatg ctgaaaatca agtataatac cataggaaca aactggaagg tgaaaaagag
    44401 catggcacaa ctatttttta agtatggcgc aatgaatagc ggcaagacga tcgagatcct
    44461 aaaagtcgca cataattatg aagaacaaaa taagccagtt gtcttaatga caagcggtat
    44521 tgatacaaga gatggtttag gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat
    44581 gccgatattt gaagaaacag acgtatttga cgtaattcag aaaatggaaa gcaaacccta
    44641 ttgtgtgctg atcgatgaag cgcaattctt aaaaaagaaa catgtcattg cttttacgag
    44701 aattgtggac gaattggata ttccggttat ggcatttggt ttgaaaaatg attttcgaaa
    44761 cgagttattt gaaggatcaa aatatttatt gctttatgca gacaaaatcg aagaaatgaa
    44821 aacaatctgt tggttctgcc acaaaaaagc aatcatgaat ttacattata ttgatggaaa
    44881 ccctgtttat gaaggcgatc aagtgcaaat tggcggaaat gaagcttatt accccgtttg
    44941 tcgaaaacat tactttcacc catcaatgat tacagaagga gaataagcat gtacgatcaa
    45001 ttacaagcaa ttgaagaccg ttatgaagag cttggagaac ttttaagtga cccagaagtg
    45061 atttctgata caaagcgttt tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca
    45121 gtcgaagttt atcgtcgtta taaagaagta gtacaaggaa tcaccgatac agaagaactg
    45181 cttggtgaaa aactggatga tgaaatggct gaactagcaa aagaagaatt atctgaatta
    45241 aaaaaagaaa aagaagtctt agaagaaaaa atcaagatct tgcttttacc aaaagatcca
    45301 aatgacgata aaaatatcat catggaaatc cgtggagcag caggcggaga cgaagcagca
    45361 ttattcgctg gcgatctttt caatatgtac caaaagtatg ctgaagcaca aggctggaaa
    45421 acagaagtga tggaagctaa cgttaccgga atcggtggat ataaagaagt gatcatgatg
    45481 atcaccggag acaacgtata ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag
    45541 cgtgttcctt caactgaatc gcaagggcgt atccatactt ccaccgcaac agttgtcgtg
    45601 atgccagaag ctgaagaagt agagatcgat attgaagaca aagatatccg tacagatatc
    45661 tatcatgctt caggtgctgg cggacagcac gtcaataaaa cagcatctgc tgtacgtttg
    45721 actcacttac caacagggat cgttgttgct atgcaagatg aacgttctca aatcaagaac
    45781 cgtgaaaaag caatgaaaat cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca
    45841 caaagcgagt atgatgccaa tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg
    45901 atccggacgt ataatttccc gcaaaatcgt gtgactgatc atcgtatcgg attaactatc
    45961 caaaaattag atcagatttt agcagggaaa ttagatgaga tcgttgatgc attgatcatg
    46021 tatgatcaaa catcaaaact ggaagctatg caaaatgggt aaaatgacct atcgagaagt
    46081 cctgtcacgg gcttcttctt ttttagagga tcatgggaaa gaagcatata gtatccaatt
    46141 tttatttttg gaacgaaaac aatggaaaaa acttgattgg ctccttcata tgaacgaaga
    46201 gatttcagaa gaagaacagc ggttgatcga aaccgatctg cgacttctgc ttgcagatca
    46261 tccacctcag tatttgctag gatatgcaga tttttatgat catcggttaa aggtaactga
    46321 agcaacactg atccctcgac cagaaacaga ggagttagtc gaatggtgtt tagacgagac
    46381 gcctggtgta ccgttagaag tgatagatat tgggacaggg actggagcaa ttgctattag
    46441 tttgaaagca gcaagaaaaa attggcatat atctgctgtg gatctttcag aagaagcatt
    46501 agaagtagcg aaagaaaatg ctcagaaaga aggaacgaaa atcagttttt atcatggaga
    46561 tacattagga ccagtaatgg atcagcaatt tgatgtgatt atttctaatc cgccgtacat
    46621 cagtcggaat gaatgggaac tgatggatga aagtgtccgg tcattcgaac ctaaaatggc
    46681 cctttttgct gagaacgatg gattagctat ttatgaaaaa atagcagttg aagcaccaag
    46741 cgtactaaaa tcaaatggaa agattttttt agaaatcgga tttcgccaag gtgaagcagt
    46801 aaaaaaaata tttcagcaag catttccaga taaaaaagtg gcgataaaaa aggatctttt
    46861 tggcaatgaa cgaatgatcc aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt
    46921 gtttttttat tataaaaaaa cacttatttg acaaggatgt aaacgacttt ttggtttatc
    46981 aacaagttta tccactattg acaaatagat tatccacaaa cagtggaaaa cttgaggata
    47041 aactcagaca aaataagaat gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac
    47101 gatatgatga aacagaattg caagaggcag ccgaagcact gaaagaagga gaacttgttg
    47161 cttttcctac cgaaacagta tacggattgg gcgccaacgc tcttttacca aacgctgtca
    47221 agaaagtgtt ttctgtaaaa gggagaccgc aagataatcc attaatcgtc catgtcgcct
    47281 cttttgaaca agtaaaagag tatgtggaca actttcatcc ggcagcagag aagattgtga
    47341 agaacttctg gcctggacca ttaactttga tatttaagat caaaaaagac actttgccct
    47401 cagttgtgac gggaggatta tccacagctg catttcgtat accggataac aaaaaaacac
    47461 tagaagtaat cgaactttcc ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa
    47521 aaccaagtcc aacgactgca gatcatgtat atcatgatct acaaggaaaa attaccggga
    47581 ttattgatga tggagctacg cgaattggtg tagaatccac tgttcttgat ttaagtgatc
    47641 caacagccat gcctatgatt ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag
    47701 tcatcgaatc tcctgttgct attgatcagc atcttgtaaa agaaaatgaa acaccaaaag
    47761 cccctgggat gaaatacaaa cactactcac ccgatacacg tgttctgatg gtcagagatg
    47821 gcgattggtc aactgcagtt caatgggcaa aaaataagaa gatccgtgca ggtgtcatag
    47881 caagccccga aattgcggat caagtgcgga cagatactgc agctgtttat atgtataacg
    47941 acaactcagt agaagcggcg gctaaaggat tgtttgcagg tctaagagga ttagatgaac
    48001 caacacttgg attagatttg atttttgtac aagtgtatcc agaaactgga ttggggaatg
    48061 cttacatgaa tcgcttgaaa aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg
    48121 gttattgtcg cattctttta gtatgataca atgctatcaa gtaaatcttc tatcagtgaa
    48181 cgaaaaacta agcgtaacta atcgtaatag aagagatacg aatatttggc aatttgtttt
    48241 tgatgggaaa aataaaattg taggaaaata aaaaggagct gttgtttgtg gtagattaca
    48301 aaacgtttga ccctgatctt tgggctgcga tcgccaaaga agaagaaaga caagaacata
    48361 atttggagct gattgcctct gaaaactttg tctccgaagc agtgatggct gcacaaggaa
    48421 gtatcttgac aaataaatac gcagaaggtt atccagggca tcgctattat ggtgggtgtg
    48481 aatttgtcga tatcgttgaa aatttagcga ttgaccgtgc aaaagaatta ttcggagcga
    48541 aatttgcgaa cgtccagcct cactccggct ctcaagccaa cacggctgct tatctcgctt
    48601 tggtagaacc aggggacacg atcttaggaa tggatctttc cgcaggcgga catctaacac
    48661 atggatcacc agtcaacttc agtggcaaaa cctatcactt tgttgcttac ggtgttgatc
    48721 cgacaacaga agttattgat tataatgtgg tacgtatttt agctagaaaa catcaaccaa
    48781 aattgatcgt agcaggtgcc agtgcttacg gacgaacgat cgactttgcg aaattcagag
    48841 aaatcgcaga tgaagtaggc gcaaaactga tggtagatat ggcacatatc gcaggattag
    48901 tagcagcagg acttcatcct aatccagttc cttatgcaga tatcacaacg actaccactc
    48961 ataaaacatt gcgtggccct cgaggcggca tgattttgac aaacgatgaa gcattagcaa
    49021 aaaaaataaa tagtgcggta tttcctggta ttcaaggcgg ccctctagag catgtgattg
    49081 cgggtaaagc agtagcattc aaagaagcat tagatcctgc atttaaagaa tatagtgaac
    49141 aaatcatcgc caatgcaaaa gcaatggtca aagttttcaa tcaagcaatt ggaaccagag
    49201 tcatttcagg ggcgacagat aatcatctga tgttgatcga tgttcgcgag ttaggtataa
    49261 atggaaaaga agcagagagc attttggaca gtgtgaatat cactgtaaat aaaaactcga
    49321 ttccttttga aacactaagt ccattcaaaa caagcggtat tcgaatcggc acgccagcaa
    49381 tcactactcg cgggttcaaa gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag
    49441 cattacaagc aaaagatgat aacgctcaac tagacgaagt caaaacaggt gtacgcgaat
    49501 taactgaaaa atttccgttg cataaaaaat aaaaatttaa attccattag taaacagaaa
    49561 agttcaatgt tgggcttttc tgtttatttt ttgggaaaac ggattctttg acaatatcgg
    49621 tgatgctctc tagtatatga ggtgtaattt tagtcttcac actaaatctc agggaggaaa
    49681 agtcatgact gatcgtatct cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc
    49741 gcctcatccg cgtatctatg aactagcacc ggaagaagga agagttttac tagaagaagt
    49801 acaagaatca cctgtgaaca aatattccgt cgatattgaa gacttgatgg tggatactgg
    49861 cgaatgggga aaactcaata ttcgtctcgt acgaccagaa gggaatatgg ataaattacc
    49921 agttattttt tatattcatg gagcaggctg ggtttttggc agtgctcaaa cccatgataa
    49981 attgatcaga gaacttgcgg ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg
    50041 atcaccagaa gcaaaatatc caacagcaat tgaacaaaat tatgctgtgt tgcaacagct
    50101 gaataaatta gccaagtcaa aaaatctgga tctcgagaat ctaatcgttg caggagattc
    50161 agtaggtggt aacatggcta cagtcatgac gatcttaaca aaagaacgaa agggcctccc
    50221 aatatccaag caattgctgt attatccagt cacaaacgct gattttgata cagattccta
    50281 tcatgaattc ggagaaaact attttttgac aagagaagga atgatgtggt tttgggacca
    50341 atatacgacg aatgaagaag agcgtgctga aatcacagct tcgcctttga gagcaaatat
    50401 aaatgaacta aatgatcttc cttcagcaat gattttaacg ggagaagcgg atgtattaag
    50461 agatgaaggt gaggcttatg cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt
    50521 acgcttccaa ggaatcatcc atgattttgt catggtaaat gctatggatc agacaaaggc
    50581 aacgcgtgca gcaatggcat tatctacttc ttggatcaag aaaaaagaat aagctttgct
    50641 tgtccgcagg aaaacttctt ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac
    50701 tagtcaatca cttgattttg ttttacaatt aaaaagaaac gacagaacaa aggagagctt
    50761 aaaaaatggg caaatttcaa gtgatcgatc atccattgat tcaacacaaa ctaactatta
    50821 ttcgggacaa aaactgcgga acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt
    50881 tgatggcata tgaagtatca cgggatatgc cattggaaga catcgtgatc gaaacaccga
    50941 tcactgaaac aacacaaaaa acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc
    51001 gcgcaggtat tggaatggtt gacggtatct tggaattgat tccagctgct aaagtgggac
    51061 atatcggtct ttatcgagat gaagaaacat tggaaccaca tgaatatttc gtcaaacttc
    51121 cagaagacat tgatgcacgt cagttgtttg tagtagatcc aatgttggca acaggcggat
    51181 cagcaatcat ggcaatcgat gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt
    51241 gtttggtagc agcacctgag ggagtaaaag ctttgcaaga agcgcatcct gatattgaca
    51301 tctatacagc ttctctagat gaaaaattga atgaacatgg ttatatcgtt cctggactag
    51361 gggatgcggg tgaccgtttg tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac
    51421 aacttttgtc acatcctttt ttggtcattt ttaacgtttg atgcagaaca gctttttcac
    51481 agctttcgtt tgcatataaa aaaaccatcc caaaaatgca gagatggtca aattcaaatc
    51541 ttttttagtt ctttgtagtt aacgattact acacccttac tttgcgtcat caagttcgca
    51601 ttttctgcag tttcatcaat ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt
    51661 ccagtttgcg acttgccgtg aattgtgaac attacgtgag tattgatctt gaatttttca
    51721 ccaatttccg atggtacgat agttgtatca aattttccaa aagttgccat tttcataaaa
    51781 cctgcctttc acatcaatta taccacgttt tatgaaaaat atcatgaaat taaactaatt
    51841 ttccatcgga ctctttacaa tcagccaaat agagtatata atttcttttg gataaggcca
    51901 gcttagctca gttggtagag caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta
    51961 tagctggcat taaaagagaa gacagatata tcaaggattt ttaatccatt gatacatctg
    52021 tctttttgtt taaaggaaat aaagtatgct ttcttagcaa tagagcatgt attatttcta
    52081 taaaaattta ggagaataaa acgactagca atatagctgt gaaatcaaat atcacatgaa
    52141 ctgcccaact tgtaatgatc gagttgcttt taatggcagc ttggttgaat aacaatcttg
    52201 ccaccccttg tatcaaaagg atgtgtgcta cagtgtgtaa aatatttcca ttatcataag
    52261 tactgtaatg gataaatcca aagatgattg ctgacaagaa actagcgaca ggaatgctga
    52321 acttactaga aaaaatagcg agaaaataca ctgagaacaa ttcttctcca agtatcatga
    52381 taggcaaaat aataaataat aacgggctga aagcctcatt tattggattg cctgttaaac
    52441 cccatttcaa aatttgttgc aaaaataccg agactgcggc actgattact acattgataa
    52501 aaaaatattt ggctatattt ttgacaggag cgacaggttt cgagaacatt tttttcgtat
    52561 cttttttacc aaaaacaagt gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc
    52621 taataatgga tggcgcaaat agcaacccta acaaaccgag tacaacgaca atagtgccca
    52681 atatcccttt tttaagttct attttttttc ctaaaatgtt attcatgata ataactccta
    52741 ctattcagtt tttgaagata gagtcagtat acat
//
LOCUS       DADXTG010000004        51512 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.004, whole genome shotgun sequence.
ACCESSION   DADXTG010000004 DADXTG010000000
VERSION     DADXTG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.004"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..461
                     /locus_tag="IWI57_000164"
     CDS             <1..461
                     /locus_tag="IWI57_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448338.1"
                     /translation="FGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGT
                     WIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRT
                     YYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(701..784)
                     /locus_tag="IWI57_000165"
     tRNA            complement(701..784)
                     /locus_tag="IWI57_000165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Leu,seq:gag)
     gene            839..1018
                     /locus_tag="IWI57_000166"
     CDS             839..1018
                     /locus_tag="IWI57_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448339.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1167..2384
                     /locus_tag="IWI57_000167"
     CDS             1167..2384
                     /locus_tag="IWI57_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBC2448340.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2568..3404
                     /locus_tag="IWI57_000168"
     CDS             2568..3404
                     /locus_tag="IWI57_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2448341.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3480..4628
                     /gene="nagA"
                     /locus_tag="IWI57_000169"
     CDS             3480..4628
                     /gene="nagA"
                     /locus_tag="IWI57_000169"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBC2448342.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4848..5906
                     /locus_tag="IWI57_000170"
     CDS             4848..5906
                     /locus_tag="IWI57_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBC2448343.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            5910..6944
                     /locus_tag="IWI57_000171"
     CDS             5910..6944
                     /locus_tag="IWI57_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBC2448344.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7046..7762)
                     /locus_tag="IWI57_000172"
     CDS             complement(7046..7762)
                     /locus_tag="IWI57_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBC2448345.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7731..8516)
                     /locus_tag="IWI57_000173"
     CDS             complement(7731..8516)
                     /locus_tag="IWI57_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBC2448346.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8507..9256)
                     /locus_tag="IWI57_000174"
     CDS             complement(8507..9256)
                     /locus_tag="IWI57_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBC2448347.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9554..10078
                     /locus_tag="IWI57_000175"
     CDS             9554..10078
                     /locus_tag="IWI57_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBC2448348.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10094..11593
                     /locus_tag="IWI57_000176"
     CDS             10094..11593
                     /locus_tag="IWI57_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBC2448349.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11681..12283)
                     /locus_tag="IWI57_000177"
     CDS             complement(11681..12283)
                     /locus_tag="IWI57_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBC2448350.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12387..13343
                     /locus_tag="IWI57_000178"
     CDS             12387..13343
                     /locus_tag="IWI57_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBC2448351.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13648..14604)
                     /locus_tag="IWI57_000179"
     CDS             complement(13648..14604)
                     /locus_tag="IWI57_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2448352.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14778..15338
                     /locus_tag="IWI57_000180"
     CDS             14778..15338
                     /locus_tag="IWI57_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448353.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15331..16155
                     /locus_tag="IWI57_000181"
     CDS             15331..16155
                     /locus_tag="IWI57_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBC2448354.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16165..16782
                     /locus_tag="IWI57_000182"
     CDS             16165..16782
                     /locus_tag="IWI57_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBC2448355.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16800..17483
                     /locus_tag="IWI57_000183"
     CDS             16800..17483
                     /locus_tag="IWI57_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBC2448356.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18166..19401
                     /locus_tag="IWI57_000184"
                     /pseudo
     CDS             18166..19401
                     /locus_tag="IWI57_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19409..20296
                     /locus_tag="IWI57_000185"
     CDS             19409..20296
                     /locus_tag="IWI57_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2448357.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20313..21149
                     /locus_tag="IWI57_000186"
     CDS             20313..21149
                     /locus_tag="IWI57_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2448358.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21152..21775
                     /locus_tag="IWI57_000187"
     CDS             21152..21775
                     /locus_tag="IWI57_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448359.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21772..23064
                     /locus_tag="IWI57_000188"
     CDS             21772..23064
                     /locus_tag="IWI57_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2448360.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23423..23520
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23636..24217
                     /locus_tag="IWI57_000189"
     CDS             23636..24217
                     /locus_tag="IWI57_000189"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBC2448361.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24220..25527
                     /locus_tag="IWI57_000190"
     CDS             24220..25527
                     /locus_tag="IWI57_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBC2448362.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            25820..26308
                     /gene="purE"
                     /locus_tag="IWI57_000191"
     CDS             25820..26308
                     /gene="purE"
                     /locus_tag="IWI57_000191"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBC2448363.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26301..27425
                     /gene="purK"
                     /locus_tag="IWI57_000192"
     CDS             26301..27425
                     /gene="purK"
                     /locus_tag="IWI57_000192"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBC2448364.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27438..28733
                     /gene="purB"
                     /locus_tag="IWI57_000193"
     CDS             27438..28733
                     /gene="purB"
                     /locus_tag="IWI57_000193"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBC2448365.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(28913..29290)
                     /locus_tag="IWI57_000194"
     CDS             complement(28913..29290)
                     /locus_tag="IWI57_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448366.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29546..30274
                     /locus_tag="IWI57_000195"
     CDS             29546..30274
                     /locus_tag="IWI57_000195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBC2448367.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30274..30528
                     /gene="purS"
                     /locus_tag="IWI57_000196"
     CDS             30274..30528
                     /gene="purS"
                     /locus_tag="IWI57_000196"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBC2448368.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30530..31201
                     /gene="purQ"
                     /locus_tag="IWI57_000197"
     CDS             30530..31201
                     /gene="purQ"
                     /locus_tag="IWI57_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBC2448369.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31202..33424
                     /gene="purL"
                     /locus_tag="IWI57_000198"
     CDS             31202..33424
                     /gene="purL"
                     /locus_tag="IWI57_000198"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBC2448370.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33409..34848
                     /locus_tag="IWI57_000199"
     CDS             33409..34848
                     /locus_tag="IWI57_000199"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBC2448371.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            34871..35923
                     /locus_tag="IWI57_000200"
     CDS             34871..35923
                     /locus_tag="IWI57_000200"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBC2448372.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            35920..36498
                     /gene="purN"
                     /locus_tag="IWI57_000201"
     CDS             35920..36498
                     /gene="purN"
                     /locus_tag="IWI57_000201"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBC2448373.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36495..38030
                     /gene="purH"
                     /locus_tag="IWI57_000202"
     CDS             36495..38030
                     /gene="purH"
                     /locus_tag="IWI57_000202"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBC2448374.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38046..39320
                     /gene="purD"
                     /locus_tag="IWI57_000203"
     CDS             38046..39320
                     /gene="purD"
                     /locus_tag="IWI57_000203"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBC2448375.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39442..40356
                     /locus_tag="IWI57_000204"
     CDS             39442..40356
                     /locus_tag="IWI57_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBC2448376.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40489..42054)
                     /locus_tag="IWI57_000205"
     CDS             complement(40489..42054)
                     /locus_tag="IWI57_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBC2448377.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42047..43498)
                     /locus_tag="IWI57_000206"
     CDS             complement(42047..43498)
                     /locus_tag="IWI57_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBC2448378.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43512..44528)
                     /locus_tag="IWI57_000207"
     CDS             complement(43512..44528)
                     /locus_tag="IWI57_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2448379.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            44748..45836
                     /locus_tag="IWI57_000208"
     CDS             44748..45836
                     /locus_tag="IWI57_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBC2448380.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            45890..47416
                     /locus_tag="IWI57_000209"
     CDS             45890..47416
                     /locus_tag="IWI57_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBC2448381.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47418..48623
                     /locus_tag="IWI57_000210"
     CDS             47418..48623
                     /locus_tag="IWI57_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2448382.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            48880..49857
                     /gene="guaC"
                     /locus_tag="IWI57_000211"
     CDS             48880..49857
                     /gene="guaC"
                     /locus_tag="IWI57_000211"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBC2448383.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50121..>51512
                     /locus_tag="IWI57_000212"
     CDS             50121..>51512
                     /locus_tag="IWI57_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBC2448384.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSK"
BASE COUNT        16424 a         8792 c        11317 g        14979 t
ORIGIN      
        1 attttggtaa tacaatgggt caaagcttta cttacgtcaa tggaaataaa agggcaatga
       61 tttggagtag tagtaacctg aataatctct ttacagagtt aggaacgaaa ctgtcacttt
      121 tttatccagg tacttggata gctactttag tagtcttggc attattacta tgtttaattg
      181 ttggaattta tttaagtttt gacaataaga aaacttctat ttttttagtt attttgatga
      241 taatgggatt ttgttcaagg ttaattatgg gattttctcc cacagtatgg gcttccggaa
      301 tgcggacgta ctatatactt tatgttgtta tcgccatctt agtactaatg gcagttaaag
      361 agctaatgaa aagcatgagt gtacaaaaaa atgaatttat gcaatttgga ttaaccgtgc
      421 ttggtatttg tacctttata ataacggtga taaacagata atatacaaaa aatccctcgc
      481 tttagtatta ggtgagggat tttactcgtt tttcgattaa ttgttttaac acttgcaagt
      541 ttctacgagt agcttgtttc cgaccttcat atactcacct caggctgttt acatgattgt
      601 acgttctttt tgtaaaggat acagtgatat gtatataatg gcataaagaa acgttcaaac
      661 aaaaaaactc gtgaagcaaa attaacttca cgagttgtag tgcagatgat gggactcgaa
      721 cccacacgag ctaacgctca catgaccctc aatcatgctt gtctgccaat tccaacacat
      781 ctgcgtaata ataacacact aatgatagcg aggttttaca tagttgtcaa gctcaaaaat
      841 gggaggactt attaagatgg agaaatatga cggagaattt tcaggactgg gaatgatctt
      901 aggtgtattg attggacttg cattcggacg ttttttgctt ggttttatgc taggaatcat
      961 ttgtggaatt gcaatggatt gggcagcgaa tctatggaat gactatcatg acaattaagt
     1021 tctcaacgct tgtttgtgtt tttttaatac ttacgttttt cctaaataag tggcaaaatt
     1081 ttctaggatg gagtatgata ttgaaatagt aaataacaaa aaatttggaa agttctaact
     1141 ttttgaaatg agggattggc taagtgatga gagaatttga aaaatccaac aaattagaag
     1201 gtgtcagtta cgatgttcgc ggtcccgttc tagaagaagc ggagcggatg caagaagagg
     1261 gcattagtat tttaaaattg aacacgggaa atcccgcacc ttttggattt gaagcaccaa
     1321 acgaagtcat tcgtgatttg atcatgaatg cccgtgactc agaaggctac tcagattcca
     1381 aggggatctt ttctgctaga aaagcaattg agcaatattg ccaactgaaa cattttccga
     1441 atgtgacgat caatgatatc tacaccggca acggagtgag cgaattgatc acgatgtgta
     1501 tgcaggggtt gctggacaat ggagatgaag tactggttcc aatgccggac tatccgttgt
     1561 ggacggcttc tattgcatta gctggaggca cacctgttca ttatatttgc gatgaagagg
     1621 cagaatggta tcctgatatt gatgatatca aatcaaaaat aacgagtcgg acaaaagcta
     1681 ttgttatcat taatcctaat aatcctactg gggcacttta tccaaaagaa ttattagaag
     1741 aaatcgttga agtagctcga caaaataacc tgattattta ttccgacgaa atctatgatc
     1801 gcttagtgat ggatggtcta gaacatattc cgattgctac attagcgcct gatttgttcg
     1861 ttgttacgct gaacggtcta tccaaatctc atcgtgtagc aggatttcga gtaggctgga
     1921 tggtcctaag tggagataag tcacacgtca aaggctatat agaagggctg aatatgttgt
     1981 cctccatgcg tttgtgttcc aatgtattgt ctcagcaaat tatccaaacc gcgctaggcg
     2041 gttatcaaag tgtcgatgag ctgctcctgc caggcggaag gatctatgaa caaagagaat
     2101 atatctataa tgcaatcaat gatattcctg gtctttcagc cgtaaaacca aaagctgctt
     2161 tttatatatt cccaaaaatt gatacaaagc gattcaatat tttggatgat gaaaaattcg
     2221 ttttggactt tttacatgaa catcatattc tacttgtaca tggtggtgga tttaattgga
     2281 accagccaga tcattttcga atcgtttatc ttcctaaaat ggatgatttg aaacaaacag
     2341 ctaagaaaat gagagaattc ctagcaacct atcgccaaaa atgattttaa aaaccaaatt
     2401 tttcagtagg agggccacag acaaacacac tgaggctctc tttctagtgg agttttctta
     2461 aataactaaa gtatattatt tagttgcaag tcttttcttg aagtttgcga cttcctaaag
     2521 taaaatacga atagaatgcg aatacgcata ttttggggag gatacttatg gaaacgttaa
     2581 tcgagtcctt tggaacgatc attaaagaga ttcgaaaaga acaaaaaatg acgcaaaaaa
     2641 tgctttcgca tgatatttgt tcacaaagtg tattaagtcg gatcgaaaat aatgaggaac
     2701 tgccgaatgt attggtgatg atgcagattt gtcaacggct aggggtaacg attgatcatg
     2761 tgatgaatct ctctcgaaaa actactcgga caaacgataa attatttgaa ctgttggata
     2821 gttgtttcca aaaacgtgat tatcggaagt tagaacaagt actaaaatct tctgatattt
     2881 ctgaaaatct gtatcaagca actgactttc aacgctatta ttattatctc ggtgtttgcg
     2941 aattcacgtt gcgtcaaaat atctctcagt ccctttatta tttgaaagaa gcattaagct
     3001 actctagcca aaaagaaaga atccatgttt ctgacacaga aatacaactg attagttgta
     3061 tcggaaaaat atatggtgtg gcaggaaaaa cggctgaagc acgctactat ttgagtcgaa
     3121 gcatccaact gctgcatcaa tcagctgatg aacgatcaaa aagtgaattt tcgcaaattt
     3181 tttataatta tggaaatttc ttattccatc aaaatgaaat cgacgaagcg ttagaacagg
     3241 tgaatcaagg aatctattgg gctcaagaga agaatagcta ctattacctc aatgatttat
     3301 ttgtgcttaa aagcttaata tacaagcgta aagaagaacc agaaagagca gcattctatg
     3361 aaggcttagc acaagctgtg aaacaaattt caaaaaattt gtaaatggtg tagaccattc
     3421 ttgacttctt ctctaaataa gagtacttta gaggtagaaa aacaaaggga gagaatagaa
     3481 tgagaacttt tatctatgct gacaaattct ttttagcgtc aggtgtcaaa catgcaggct
     3541 atcttgaaat taccgatgga ctttttggtg aatacaccaa agaactacct gagggagaag
     3601 tcaacatcat tgaccaaagc ggcaaatgga ttgctcctgg tttggtagat acacatattc
     3661 acggctatat gaatcatgat gtaatggata atgatgcaga aggcatcaaa gtgatgtctg
     3721 aaggtctgtt atcttgtgga gtgacttcat ttttaccaac aacattgacg tcaagcaaag
     3781 aacgtttaaa agatgtagct gagacaatcg gcaaaatgta tcaggaagca ccaggagcta
     3841 aaatcaaagg aatctatttt gaaggtcctt tcttcacaga agaacacaaa ggggcacaaa
     3901 acccaagtta tttcggggat cctgatttgg atactttcca tgaatggcaa gaagcttcag
     3961 gtggtttaat caaaaaaatt gctttagcac cggaacgtca aggtgtagaa gagtttgtca
     4021 aacaagtgac tgaagaagga gtagttgttt ctctaggaca cagtaatgct acactagaag
     4081 aagcgcaaaa agctgttgaa gcaggcgcaa gtgtatttgt ccatgctttc aatggtatgc
     4141 gtggattgaa tcatcgcgaa ccaggtatgg tgggtgcgtt attatcttta gaacatgtct
     4201 tttctgaatt gatttgtgat ggccatcatg tacatccaaa tgctgcggga atcctaatgg
     4261 aaaaagcagg acatgatcat gtcgcattaa ttacagactg tatgatggcc ggtgggatgc
     4321 ctgatggaaa ttacaattta ggcgaatttc cagtagtagt aaaagaagga acagcacgtc
     4381 tagaatctgg caacttagct ggaagtatat tgaaattgaa agaagcaatt aaaaatgttg
     4441 tagactgggg attagcaaca ccagaacaag cagtcatgat ggctagtctg attccagcaa
     4501 tcagctgtaa aatcgatgat acatgtggaa aaatcgctca aggacgcgat gcagacttta
     4561 tcgtattgaa ccctgatatg acattggaag caacttatct agatggcgtg gaacgtttcc
     4621 acgcataata taatttaagt taaccttaaa actaaacttc ttaagtttgg ttttaaggtt
     4681 tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt aatttcctat
     4741 tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat tagcaaaata
     4801 aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg aacttatttg
     4861 acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact tcaacagttt
     4921 ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa tctgttggaa
     4981 aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt cgagctttca
     5041 tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt tggggattag
     5101 ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat aaaggggata
     5161 agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc ggaacgccta
     5221 tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc aatattggct
     5281 accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag atggaagcta
     5341 aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc ggccttttca
     5401 tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa ggagagataa
     5461 gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag ccgatggatt
     5521 atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca ttaggtgaag
     5581 taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta gtcaaagtgg
     5641 tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg aatattctac
     5701 cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca gctgcaacag
     5761 tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc aatgcatcct
     5821 tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta ttacaaatga
     5881 ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga atcgcatgtt
     5941 acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac cattatgatc
     6001 tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag gaccatgatg
     6061 ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa atgcaggaat
     6121 atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat ctacaagcgg
     6181 cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat gcgatagcag
     6241 agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg acgatccgaa
     6301 catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc agaaattgca
     6361 cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg tttaagggac
     6421 taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa aaagtattga
     6481 cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt catgtacctc
     6541 atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa atgaaaaaag
     6601 gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct attttaagag
     6661 ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat gaaacagagg
     6721 tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc cagcaattag
     6781 tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca gatgaggcat
     6841 tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa actggaaaaa
     6901 cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac atggatattc
     6961 gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta tacttggact
     7021 attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc cgcccattct
     7081 ttgtcaattc ataacaggtt agcggctggc cgatttccaa atatttgatt ctttcgttca
     7141 atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt gacagtgagc
     7201 ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata aatgcactta
     7261 ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat ggtatttcgt
     7321 gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct ttttcagtcg
     7381 tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa cgttgcaaag
     7441 tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt atggtttcga
     7501 tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt ctaagccaag
     7561 ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc agatctgcgt
     7621 ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg attacgttca
     7681 tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga tcatcttcga
     7741 ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag atagacgatt
     7801 gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt gacgattgaa
     7861 tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc tagcgtaaat
     7921 attttttctg agatttcttc tgatatcccg acaccgccat cgcagacggt cacgatgaat
     7981 acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct ttcatgtttt
     8041 tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa aagtgaatgg
     8101 atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag catttcaaca
     8161 gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc aggtactttg
     8221 atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg ctggataaaa
     8281 tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg aagtttattc
     8341 atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc cccaagctcc
     8401 tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc taaaaaacgg
     8461 tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa atacatggat
     8521 tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc atatagatta
     8581 tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag aaaatgtttt
     8641 ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag attccttcat
     8701 ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct tgtggttcaa
     8761 gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg atgcctacgc
     8821 taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact tgattgtcaa
     8881 tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca taataaatag
     8941 ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa aattcttgtc
     9001 ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg taacttgggt
     9061 ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct aaattcgatg
     9121 caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca gtctgcagct
     9181 ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt ttatctgaca
     9241 cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa acaatcaaag
     9301 aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt aatggttttt
     9361 tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat tttaacatga
     9421 aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa aacaccactg
     9481 agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg tagaaatgga
     9541 aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt atacattgct
     9601 gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg attttgatgg
     9661 cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat ttggttttct
     9721 aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg gaggcatatc
     9781 gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac atgccgaatc
     9841 aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga tcacaccgat
     9901 ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca ttagtcgtcc
     9961 agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag tattgatcat
    10021 tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa aagcttaaag
    10081 gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg cagctttgat
    10141 tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac cggatgaagc
    10201 gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg aaagcaataa
    10261 caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc aacgttcgaa
    10321 tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt atacggaaca
    10381 aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat ctgtagaaga
    10441 gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg aaaatgaagc
    10501 aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg tagaagaaat
    10561 ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg tagaccttgc
    10621 aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca aaaacggtta
    10681 tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag atatagcaga
    10741 agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag aatcacaagc
    10801 agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag aactgaaatt
    10861 agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag cctacaattt
    10921 agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta aaatcgtaga
    10981 acgccaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa agcaatatga
    11041 ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag aagcacaagc
    11101 tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg aagcactggc
    11161 agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag cagcactagc
    11221 aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca aggaatatgg
    11281 cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc gcgaagcagc
    11341 acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca gttcaggaga
    11401 aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt ctactcaaga
    11461 aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt tgtcgacacg
    11521 tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac aaaactcgat
    11581 gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa gactttttaa
    11641 agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg ctatttttct
    11701 tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat cgttctttct
    11761 tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca ggaggacgaa
    11821 gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt acacgttgga
    11881 tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac aacgtagcta
    11941 ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc acaataataa
    12001 ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag cttttgacaa
    12061 aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta gactttccaa
    12121 aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat gcagcaatca
    12181 tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca gctaaggcac
    12241 ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt ctcctttttg
    12301 gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt attcaagtat
    12361 aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc aaaacagtga
    12421 tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat tggccagctg
    12481 cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg cgtacgggca
    12541 tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt ttttttgctg
    12601 ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg cttgcctctc
    12661 gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat tatttagtgg
    12721 aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg attggattag
    12781 tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg aaggaaaacc
    12841 aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa tttcttttat
    12901 cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc attcgcgaaa
    12961 gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg ctatactacg
    13021 aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg ctggaaaatc
    13081 tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta ttgatttata
    13141 ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt attgaagaag
    13201 tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc gaggatcgag
    13261 cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag gcagaattct
    13321 cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg tcaaaaagct
    13381 gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc acaagtcaca
    13441 atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc ggagctgaac
    13501 ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc ggtaataagt
    13561 ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt acttgatgca
    13621 gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt gcatttgatc
    13681 caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt caccttcaat
    13741 catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc cgctttcgat
    13801 gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg ggtaatcagc
    13861 aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt ttgcctgaaa
    13921 agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga cggattgttc
    13981 gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt gagagctcaa
    14041 aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc ctccagctaa
    14101 ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga aaggtgcttg
    14161 gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact gctgataaag
    14221 tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt ctgctaagcg
    14281 catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata gaacgtgttt
    14341 gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta cagcaatgta
    14401 cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag cttttggaat
    14461 attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa ctgctgttag
    14521 ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac caagaccaac
    14581 gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat tgagtttctt
    14641 gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact tgaaacatta
    14701 aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag tagaatagtt
    14761 aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt gtattattag
    14821 aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt ccagaacaat
    14881 ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag aaagtaaaaa
    14941 atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa acgattattt
    15001 ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca cgcatcgcgc
    15061 aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa aataatcagc
    15121 aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg ggctatgatg
    15181 atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga gaaaaaatcg
    15241 aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag gcgaatgggg
    15301 aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt tggagcagtt
    15361 gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc gcatgttcaa
    15421 gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa agaaaagaac
    15481 agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac gtccaaacaa
    15541 ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga tcacaaatta
    15601 gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata cttgatcgac
    15661 aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc tggatttcca
    15721 ttagtgatgg tctcagctta ccggtctgta tcatcccaac aacaagtttt cgaacaaaat
    15781 gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa gattactaaa
    15841 gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt cgatgtcgtc
    15901 gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta cggagatcag
    15961 cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt tcgttatcca
    16021 aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg ttatgtcggt
    16081 aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata tctgaatgaa
    16141 ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca aaagcgcaag
    16201 aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc ctttgttttt
    16261 tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga agcgcaatcc
    16321 catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga tggttatgga
    16381 gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg aaaaagtacc
    16441 ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca gaagaaagta
    16501 agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg agacttcaaa
    16561 gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt ccaaggagta
    16621 gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca ggaagccgca
    16681 caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa aatcatccag
    16741 gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag aagtggctga
    16801 tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc gtaagagtac
    16861 cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc cgttcgatca
    16921 tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta attgctgtgt
    16981 atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct gacattccag
    17041 ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac atgagcatgt
    17101 tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct ttagagacag
    17161 taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg tcgaaatata
    17221 cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt gcaggtaaag
    17281 aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct atcattgcga
    17341 caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca aatgccatca
    17401 catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa tatcgggaaa
    17461 ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga gcagatagtg
    17521 gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa aaagagaaat
    17581 ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg ttattattag
    17641 cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg aaaaacgaat
    17701 aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg cttgaaaagt
    17761 cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac gtgtatattg
    17821 tacctaattt catacagaat tactagttat ttttctgttt tttagggaag agcgcacacg
    17881 aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat atcctttttt
    17941 ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat gaaagaagaa
    18001 tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca gaacgataag
    18061 gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt tctgtataaa
    18121 aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa gaaaagaatg
    18181 agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg gcaagaagat
    18241 gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca cgatgcaaca
    18301 gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga ttatgaattt
    18361 tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga tgatatctgg
    18421 gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga accattagat
    18481 gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta tttagcaacg
    18541 acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt tgcgattgct
    18601 tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa ttggacatgg
    18661 gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta tgctatttca
    18721 agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga gaaaggattg
    18781 aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc agactatcta
    18841 acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc agaaccaggt
    18901 ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag agaagcggca
    18961 atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc tgaagatatg
    19021 gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg tatcccttta
    19081 cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga agctgctaag
    19141 tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg agaacgtggt
    19201 gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc aactttgcaa
    19261 gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt gaatgtcttt
    19321 gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga aaaagtcatt
    19381 tatgaagaag taacagcaga aggaaggcat gaaaaaagaa aaactaaaac ggttcttgga
    19441 tcaagacaat gtggtcggtt acgtatttgc ttctccattc atcctaggat ttctattgat
    19501 aacagtgata ccgatgatct tgtcacttta ttattcgttc accgattatc gtttgggaaa
    19561 tccagtctca tggatcggct tggataatta tataaagatg ctgagtgatg cccgtttttt
    19621 gaactccatc cgagtcactt ttctctatgt atttgcatct gtacctgtga aactgatctt
    19681 tggtctattg attgcttatc ttttaacgag gaatatcaaa ggaacgactt tctttcgttc
    19741 tttgtattat gtgcctagtt tgatcggtgg cagtatttct atcgctctag tttggcggga
    19801 gttgttttct aaaaacgggt tagtgaatat tttcctggaa cgattaggcg cacctgcgct
    19861 ttcctggttt agcgatcaaa aactggtcat gatccctctt gttttgatgt ctgcttggca
    19921 attcggttcg gcaatgatca tctttgctgc aggtatcaaa gagatcccag gctcgtatta
    19981 tgaagcggca gagatcgatg gagcgaacaa atggcagacg ttttacaaga tcacgttacc
    20041 tcttctttcg ccgatcattt tgtataactt ggttatgcag acaatcgcgg cgtttatgac
    20101 ttttacgcag gcatttatca ttacccaagg tggtccgaat gatgccacga acttttatgc
    20161 tctttatgtg tataaccaag ctttttcttt taattctatg gggtatgcta gtgctatttc
    20221 gtggatcatg ctgttgatta tgggagctgt cacctttctg attttcaaat catcaaaaaa
    20281 atgggtcgtt gtgtgaatga gaggaggaag aaatgaataa tacagtaacg aaatggtttc
    20341 aatacggttt tattttgcta ttaggtctag tcatgattta tccggtctta tggatgatcg
    20401 caggttcttt taaaacggat caagagatta tgagtggttc gctgaatttg attccagaga
    20461 cttttcaatg ggaaaattac gcaaaaggat gggcggggtt tgctggaatt tcatttttta
    20521 ctttctttag gaattcgttt gtcatctctg cagtttcgac gatagggaca gttctgagct
    20581 cgacttgtat tgcttacgca ttatcgagaa tcgatttcaa aggaaaaaga ttctggttcg
    20641 tcgtcatgtt agtaacgatg atgattcctg cacaagtgat tttgattccg cagtttatta
    20701 tttacaatcg gctgaatttg gttgggacgt atgtgccgct gattttacca catttttttg
    20761 gacaagcatt tttcatttac cagatcatgc aattcatggt cagcatcccc aaagagttag
    20821 atgaatcagc gattattgac ggatgcagca agtatagtgt ctttaccaaa attatttttc
    20881 ctttgctgaa accttcgatc atcacgacga tcatcatcca gttttattgg aaatgggacg
    20941 acttcatggg accattgatt tatttaaaca agccgagatc ttacacggta tcgattgcca
    21001 tcaaattatt tgcagacgct ggatctagta cgacttacgc gagtatgttt gcgatgtcta
    21061 cgttgtcgct gctaccggtt tttctgattt tcttattctt caataaatat cttgtccagg
    21121 ggatcagtac aagcggtctg aagggctgaa tatgaaaaag ccaatagaag agatgtttct
    21181 ttacaagctc aactactatg tctgtcggat ttttttaacc agtcttatca gtaatgctct
    21241 tttcgcttta gttctaggat gtttgttcac tttaggatta gtgctgtctg ctcgtgttgt
    21301 ctatctgatt tattttttga tgatgtatcc ttcttattat ctatctgtcc attacttggc
    21361 agaaaaaaag gtatgttgga ggaaagggaa aatcaaacgg atgtgtttca tcattacgtt
    21421 gtttcagctg tttgtcatta tttttgcagt tgattggtat ttattagtgg gaaagattgg
    21481 agcttggtat ctgattcctt ttctattgtg tctgttgctt tttgtatccc atagtttttt
    21541 tagtacgcta attgatatct gcaaagaaga taaagcagat agtcttgttt atttgatcaa
    21601 aaggaatatc tggctgttgt caaaaaacgt caaggggttt ctgtggtatt tatgcctttt
    21661 gtatggactg ttctggtgta tacagcctga ttttttactc actgcattac tgtatccagg
    21721 gctgccattt gtcacagtat tttgcttaaa agaaaaggag aatgacttta aatgaagaaa
    21781 aaatatgtac aagtcggact tggtgggaga gcgcgttttt tttatgaggc aattgcctct
    21841 acgtatcatc aaacttgtga actagtcggt ttttgtgatg tatctcaaac aaggatggat
    21901 tatgcaaatc ggatacttgt gaacaagtat ggacagaaaa aagtaaagac atgtatgcca
    21961 tcacaatttg agcaactact ggatgacgta cggccggctg aagtcattat tacgtcagta
    22021 gatcgaacgc atcatgatta tatcattcgt gcaatgaaaa aaggatatga tgttatttgc
    22081 gaaaaacctc tgactatcga tgaggaaaaa gctcaagaaa taataacggt ccaaaaacag
    22141 acgggaagaa gcctgcgagt cacttttaat tatcgttatg caccgtatca cacgaagata
    22201 aaggaactga tagaaagtga tgtgattggt gatgtgtttt ctatccactt cgaatggctg
    22261 ttaaatacgc aacacggggc agattatttc agaagatggc atcgaaataa gcacaatagc
    22321 ggcgggcttc ttgttcataa atctacccat cattttgatc tagtgaattt ttggttgaac
    22381 agccgtcccc aaaaggtatt tgcattcggt ggattaaact tttatgggat gcataatgcg
    22441 gagaaacgcg gagtagaaat attttacagt cgatgctatg gaagcgaact agctaaagct
    22501 gatccatttg ctatagacct gtcagccaat gaggaattgc gagaaatgta tttagaagca
    22561 gaaaaagaca gtggatatat tcgtgaccgc agtgtatttt ccgatgatat ctctattgaa
    22621 gatacactag gcgtaacggt caaatatgaa actggtgcga ttttgacgta ttcgctaaac
    22681 gcgtatatgc cgtgggaagg attcaatgtt tctttcaatg gcagcaaagg acggatcgaa
    22741 atgaaagtga tcgaaaagtc ttacatcaat gcaggtggag ataaaaagga tgaaggagca
    22801 gccgttcttc agtccatcca tgtctatccg atgttcggtg agccatatga ggtaatcgtc
    22861 gaacaagaag cgggcggaca tggcggagga gatccgcgtt tattagatga tctatttggt
    22921 gaagaaaaag aagaagatcc gtggaatcgg gcagcaactc acgtagatgg tattttgtcg
    22981 attttgacag gaatcgcagc aaatcattct attgcttcag gaaaagcagt agtgattgac
    23041 gagcttgtca gcttaaaaga ataagaatag atttacggaa attacttccc gtagtttata
    23101 aaatcttgcc ttattgccga taggctgtct tttgaacact atttattcgg aaaaaggggc
    23161 tatgacatgc tttttgtcat gccttctttt tatgtgggct gcttttttag ataggagaaa
    23221 ataaaaataa ctatttaaac ccgaacaaaa aggaataatg cattaaaaat cattagattt
    23281 tacaaaaaaa taagtaaacc acgttgtcag tttcagaaag tagtgttaga ctgttttcaa
    23341 tatttgaaac aacatttttt attcaaatat ggcaaactgc cttcacgtgc aaaacagttg
    23401 caagaaaatg aatttacact attattagtt ttacatacct acttatatat cgtcataata
    23461 tggatgacag tttctagcca gtaccgtaaa tgctggacta taagtaaaag attggctatt
    23521 tcacccttct tttcttatga taaagtggag ggtgtttttc tttaacagaa acatagtagg
    23581 tttttgcaca aaagcggtta tttcggtatt aataaatgat aaaggagcaa ataaagtgaa
    23641 agaattagtt gaacgtattc aaaaagacgg acgtatatta ggcgagggcg tattaaaagt
    23701 cgacagcttc gtcactcacc aagtagaccc tgtattgatg gaacaaatgg gaaaacggtt
    23761 tgcagaagta tttaaagaac aaggaatcac gaaagttgtg accattgaag cttctgggat
    23821 cgcaccagct ttatttgctg caagagaatt agatgttcca atgatcttcg ctagaaaagc
    23881 aaaaagtttg acgatggatg aagaattatt gacagcttct gtttattctt ttacgaaaca
    23941 agtgacaagt acgatttcta tttctcgtaa atttttatct tcagaagaca aagtattgat
    24001 tatcgacgat ttcttggcaa acggacaagc ggcaaaagga ttgatcgaat tatgccagca
    24061 agctggagcg caagtagaag gaatcgggat cgtcatcgaa aagtctttcc aagatggacg
    24121 tcagttactt gaagaaatgg gattgagagt cgtttctctt gcgcgtattg cttccttgac
    24181 gaatggacaa gttgagtttc ttgaggagga cgcataatcg tggaaaaaga aacacaaaat
    24241 ggaaaagctg cggttttagg gctgcagcat ctgctagcga tgtatgcagg agcagtagcg
    24301 gtgcctcttt tgatcggtac aggactaggc tttaatcaag aacagatgac ttatttgatt
    24361 tcaattgata tctttatgtg tggattagcg acattattgc aattgacagt taaccgattt
    24421 ttcggaattg gcttgcctgt cgttttagga tgtgcgatcc aagcagtagc tccactgatc
    24481 ttgatcggaa ccaatgaagg agtaggagct atctacggat ctattatcgc ttctgggatc
    24541 tttgttgtgt tagtttctgg tgtcttttct aaaattaaaa aactattccc tcctatagta
    24601 acaggaacgg ttatcaccgt tattggtttg actttgattc ctgtagcaat cgagaaaatg
    24661 ggtggaggta atgcaacagc agctgatttt ggtgacaaaa caaatctgct acttgcattt
    24721 gtgacgattc tcctaattgt aggcgtgcaa atgctggcaa aagggttcat tcgttcaatc
    24781 gcggtattga ttggtttagt cggaggaagc atcttagctg ctttcatggg aatgattgat
    24841 ctgagcgcga ttggagatgc gccgttgttc catgttccac aaccctttta ttttggtaaa
    24901 ccaactttcg acgtatggtc gattttacta atgatcatca tttccatggt cagcatggta
    24961 gaatcaacag gtgtatattt tgcacttggt gacattactg ggaaaaatgt tggagaagaa
    25021 gaattgaaaa agggatatcg tgcagaagga cttgccgtta tcttaggcgg tattttcaat
    25081 acattccctt atactggctt ttctcaaaat gtcggattgg ttcaactttc gggaatcaaa
    25141 acaagaaaac ccatctattt ctctgcattc ttcttgattg ttttaggatt attccctaaa
    25201 atcggtgcgt tagcacaaat catccctgag ccggtacttg gtggaggaat gttagtcatg
    25261 ttcgggatgg tggcagtcca aggaatccgt atgctgttgg aagttgattt cagcaatgat
    25321 aaaaatctat tgattgttgc tgtttctgtt ggatttgggc tgggtttcaa tgttacgccg
    25381 acattattca gtcaaatgcc gcaaacattg caaatgttca ctggaaatgg catcgtaatg
    25441 agtagcatta cggctatcat attgaatttg atctttaatg gattgaaaaa agatgaagaa
    25501 atattggaag aagccttcaa gaagtaatag agcaaaagaa aggttgtgaa atcagcgttt
    25561 tgacctgatt gttagagaaa caaacggaaa aatagttcct cgtattttgc cgtttgtttg
    25621 agctaatacc gagggtcagc tgattgaaca ccgtttatcc ataaaaagga atgtgaccta
    25681 acttttgtca cattcctttt tttcatcttt aataaacacg aacaataaaa tgtttttctt
    25741 tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata tgaatgtaga
    25801 aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga gtacttcaga
    25861 ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt cttatgaaaa
    25921 aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac aaaacgctcg
    25981 aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc tgccgggtat
    26041 ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac gaacattaaa
    26101 tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg tagctactac
    26161 agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga tgctttccat
    26221 gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg ctaaaactgt
    26281 tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg atcgggatcg
    26341 tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg ggctttaaag
    26401 taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat tggcacattg
    26461 aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca gatgtcatta
    26521 cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc atgtcattta
    26581 ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa aaatcttttt
    26641 tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg acggatatcc
    26701 aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga ggcggctatg
    26761 atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc atggaccttt
    26821 taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag atttcgatca
    26881 tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa aacatccacc
    26941 acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat gtgatcgagg
    27001 aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt gttttagggg
    27061 tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct cctcgtcctc
    27121 acaattctgg acattattct attgaagctt gttctatgag tcagttcgat gcccatattc
    27181 gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat gcagtaatgg
    27241 tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat aagccagact
    27301 ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg ggacatatca
    27361 ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg aatatttggg
    27421 attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg aatatctgga
    27481 cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat gaagcttggg
    27541 cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat gcatcatttg
    27601 atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt gcgtttacac
    27661 gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt ctaacaagta
    27721 cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat attttacggg
    27781 aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac aaatatacag
    27841 tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga ttgaaacttg
    27901 ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg gcaaaaggag
    27961 tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg ccatttattg
    28021 aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatctctact caagttctcc
    28081 ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca agtattgaac
    28141 gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta gaagaatttt
    28201 ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg atcggttcag
    28261 aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc tatgaaaatg
    28321 tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc atcttgcctg
    28381 ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt aaaaatctaa
    28441 cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg atttatagcc
    28501 agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct tatgatcttg
    28561 ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg ctgttagaag
    28621 aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc tttgattatc
    28681 atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa taaagaagag
    28741 tgagttgcaa aattcaatta aaatagaata gcaaaaataa ccaaaaaaga agcagatccg
    28801 ttagcggttc tgcttctttt agtttattat attttcttat caataatagg aacctgattt
    28861 taaaagcggc tacctcttta ccgtgcctct ttacatacaa aaaatagagg aattaagcgt
    28921 aaatggcttg gctattataa ctaaaatgct ggttatggag atgttgcagc aagttttcga
    28981 gaaaagcgta ttccatctct tgtccattga ataagatctg gatacgataa tgatttttga
    29041 aacgcttctg ttcatgaatc tgactgattg aaagttcgcc tttgtgttct gtctgataaa
    29101 gaaaagtcct tttacggcat tcagcacagc gcttggttgc tcggcggatt tttcggaatg
    29161 tataaaaact agtatcttta ttgcataaat aaacgtcttt gatcgacagc aaatcattat
    29221 atagacagga acgttcttca gctgttaatt ctgataaaaa aagctgacct tgttttattg
    29281 catcttccat cggcaagtgc ctcctcgaac ccattatcca aatttttagt taattctaat
    29341 ataccatcat taagtataaa tgtaaacgct tgttgatggt ataagatttt cgtttctgat
    29401 aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac atatttttgt tgatttataa
    29461 aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa aaacgatgta tttgctttta
    29521 atgtaaataa aaaggagaga cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc
    29581 caaaaaaata tatcttactt ctgatgagaa cttgatttgg atagaatatt tagatcaagc
    29641 aactgcgttg aatggcgcga ggaaagacca gataagtgga aaaggagaat tgaataatca
    29701 aatcacaagt gcaattttcc gttatctgac tcaaagagga ataaaaaatc attttattga
    29761 acaattgtct aaaaccgaac aattagcaaa aaaagtacaa attatccctc tggaagtggt
    29821 tatccgaaat attgcagcag gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga
    29881 attcatcaaa ccaatcattg aattctacta taaggatgac cagttggatg atccatttat
    29941 caatgaagag catatctatt tcttacaact tgcaaaaccg gaagaaatca gtgagctgaa
    30001 agcccaagcg ttggctatta atgaaatttt gaccgaatta tttgctaagg tcggtttggt
    30061 tttagtagat ttcaagctag aatttggccg gtcgaatgga caaattttgc ttgcggatga
    30121 agtaacaccg gatacttgcc gattatggga tgaacgtacg aatgaacata tggacaaaga
    30181 cgtttaccgc agagatctag gtgatttgat cccagtctat caagaagtat ataaccgtct
    30241 atcggaacta actgaagaaa aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc
    30301 tataaagatt cagtgttaga tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg
    30361 ggatatgaaa caatcgaaga tatccgaata ggaaaatact ttgagataca acttgctgat
    30421 gcagaagaac caatcgatac agtgattgaa acgatttgtg acaagctgct tgcaaatgta
    30481 aatatggaaa cataccgata tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc
    30541 ggtcatcgtt tttccaggtt caaattgtga cgcagatatg ttatgggcag ttcgcgagat
    30601 catgggcgct gatgccgaat ttgtacggca tgatgagtcc tctttagaag gatttgatgg
    30661 ggttttactg ccgggaggat tctcctatgg tgactatttg agatgcggtg cgattgctcg
    30721 gttttctaca atcatggaag aagtgattcg ttttgctgaa gaagggaaac cagtattcgg
    30781 tacatgcaat ggattccaga tattgacaga agcaggactt ttgccaggtg tactgagacg
    30841 aaatgagtct ttgcatttta tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc
    30901 ctttacttca gagtatgaag ccgacgaaat aatctatctt ccagttgctc atggggaagg
    30961 aaattatttc tgtgacgaag aaacacttca agaactaaaa aaaaataatc agattgcctt
    31021 tacttataat gacaacatca acggcagcgt ggaaaacatt gcagggatta cgaacaaagc
    31081 agggaatgtg ttaggaatga tgcctcatcc agaacgtgca gtagaagact tgctaggatc
    31141 agaagatggg aaacggttct ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata
    31201 gatgaaaaca ttgaaagaac cgacagctga agagatcaag aaaaaaagaa tctatgccga
    31261 atggggattg actgatgaag aatatcgaat gatcgaagaa gatatcttag gaagaatgcc
    31321 taattatacg gaaactggac tgttttctgt tatgtggagc gaacattgtt cctataaaaa
    31381 ttctaaaccg gttctacgca aatttcctac tagtggacct catgtgttgc aaggccctgg
    31441 agaaggcgca ggcatcgtcg acatcggtga tggactagca gttgtcttca aagcagaaag
    31501 tcacaatcat ccttcagccg tggagcctta tgaaggagct gcgacaggag ttggcggaat
    31561 catcagagat attttcagca tgggggctcg tccaattgcg ttgctagatt cattgaggtt
    31621 cggcgaatta gataatccaa gaacgaaata tttactagaa gaagtagttg caggtatcgg
    31681 cggatacggc aattgtatcg gcataccgac tgtaggcggc gaaattgcat ttgatccttg
    31741 ttatgaagga aacccattag tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat
    31801 ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg
    31861 tcgcgacggt atccacggag caacttttgc ctcagaagaa tttaatcaag aagaagaaca
    31921 gcaacgttct gctgtacaag taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg
    31981 tttggaatta atcttgcatc attcggattc tctaataggt atacaggata tgggagcagc
    32041 aggacttgtt tcatctagtg cggaaatggc ttcaaaagct ggctcaggac ttatattgac
    32101 attagatgaa gtcccacaaa gagaaactgg gatgacacct tatgaaatga tgttatcgga
    32161 atcacaagaa cggatgttga tttgtgttaa atcaggagag gaagaaaaaa tccaagaact
    32221 atttcagaag tatgatctag atgcagtcac tatcggtaaa gtaacagacg acgggcaata
    32281 tcggctatat catcaaggag aagaagtagc aaatctgcca gtcgatgcat tagctgaaga
    32341 tgcaccagtt tatcataaag aaatgaaaga accagctcga atcgtagaat ttcagcagtt
    32401 agctccttat cagccagtta tcgaagaacc tggacaagta ttgcttgatc tgctgcagca
    32461 accgacgatt gcttcaaaac gttcaatcta cgaaacttat gattcacaag tacaaaccaa
    32521 tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg gttagaggga cgaataaagc
    32581 gttagctatg accacagact gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg
    32641 acaaatagca gtggcagaag ctgctagaaa tattattgct agcggtggta agcccttggc
    32701 gatcaccgac tgcctgaact atggttctcc tgataaacca gaagtttttt gggaattatc
    32761 gacttcagct gacggaattg ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg
    32821 aaatgtctct ttatataatg aaacggatgg tcaagcaatc tatccaacac caatgattgg
    32881 tatggtcgga ttgattgagg atcataaaca tatcactact caggaattca aaaaatcagg
    32941 ggatctaatc tatatattag gtaagacgtt tgcagatttt gatggcagtg aattacaaaa
    33001 gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag
    33061 aaaccaagag ttagtattga cagccatcca aaacggattg attgaaagtg ctcatgactg
    33121 ttcagaaggt ggcttggcaa ttgcactggc agaatcagct ttcaaacatc agctagggat
    33181 atctgtacag tttgaattgt cctctgctca attatttgca gaaactcaat caagatttgt
    33241 gttgacagtc gcaccggaaa ataagacacg attcgaagaa atgatgggag atgctgctgt
    33301 tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt tcagcaactg acggtcagat
    33361 caaaatagaa actgcagtag caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa
    33421 gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt ggggtcatcc agacgccgct
    33481 agtgtggcat acttcgggct ccatagtctt cagcatcgag gacaagaagg tgcaggtatc
    33541 gtgtccaatg atcgaggaaa gcttaagggg catcgagatt tggggctgct ttctgaggta
    33601 ttcaaagatc aaagaaaatt gcagcagtta acaggagaag ctgcaatcgg acatgtacgt
    33661 tatgcaacag caggcaatgg aagtgtggac aatattcagc cttttctatt caaatttcat
    33721 gatcagtcga tcggcttagc gcataatggt aacttgatca atgctcgttc ccttagaaaa
    33781 gagttagagg aaaatggggc gatcttccat tctaactccg atacggagat attgatgcat
    33841 ttgatccgac gaagtgaaga aacagatttt ttaaataaat taaaaagtgc tttgaatgaa
    33901 gtcaaaggtg gctttgccta tttattaatg acagaaacgg caatgattgc cgctttggac
    33961 ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa acggagcata cgtgattgcc
    34021 tcggaaactt gtgcattaga agttattggg gcacgtttta taagagatgt ccagcctggt
    34081 gagatagtga tcatcaatga cgaaggaatc cagattgatt gctttacgaa ggacacgcag
    34141 ctttctattt gttcgatgga atatatctat tttgccagac cagattcaaa catcgcaggg
    34201 atcaatgtgc atactgcacg gaaaaatatg ggacggaatt tagcgaaaga atcgccagta
    34261 aaagcagata tggtcgttgg cgtacctaat tcttctttgt cagccgcaag cggttatgcg
    34321 gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca
    34381 tttatccagc caacgcctga gctaagagag caaggtgttc gaatgaaatt atctgctgta
    34441 cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg attcaattgt tcggggtacg
    34501 acaagcagac ggatcgtcca tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg
    34561 attgcttctc ctcctttgaa atatccatgt ttttacggga ttgatattca aacgcgtaaa
    34621 gaactcattg cggcaaatca cacgattgat gaaatcaaag aatgtatcgg tgcagattct
    34681 ttaggttttt taagtgaaga gggtctgatc gaatcgatcg gtctggaaaa agatgctccg
    34741 tattcaggat tgtgtatggc ttatttcaac ggagattatc cgacgccgtt atatgactat
    34801 gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag
    34861 aagggagtat atgaattcaa tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc
    34921 tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat
    34981 gatgggcgct ataggaggat tcggcggttg ttttgattta tcggtctttc aattaaaaga
    35041 gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc
    35101 agacaaacac gatacaatcg ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc
    35161 acaaggcgca gagccgttat atttcttaga ctacttagca acggggaaaa atgatccgtt
    35221 acgtttggaa aatgtagtaa aaggtgtagc agatggatgt gttcaaggaa atatggcgct
    35281 gattggcgga gaaacagctg aaatgcctgg tatgtactca ggtaatgatt atgatctagc
    35341 cggatttgct gtagggatag ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc
    35401 aggagatgtg ttgcttggaa tagcatctag cgggattcat tcaaatgggt actctcttgt
    35461 tcgcaaaatt ttctttgagc agcagcagtt ttctttatca tcaaaactgc cagaattgaa
    35521 aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc
    35581 gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc actggtggag ggtttgtaga
    35641 aaatatcccg cgaatgttac cagaatctat ggctgcacaa atcaaacttg gctcatggcc
    35701 gattcttcca atattcggct gtttacaaaa atatggtgaa attcctgaga tggagatgta
    35761 tgaaatcttc aatatgggca tcggtatggt cctggcagtc gaagaatcga aagtaaaaga
    35821 aatagtacac gaaattgagc aagctggcga aaaagtttat gaaatcggga aagtgatcgc
    35881 caaagaaaaa catcgtgtgg tgtttatagg agaagaacga tgagaatcgc tgtttttgct
    35941 tctggaaacg gaagcaactt tcaagcgcta gccgattatc tatcgaaaaa aggattggaa
    36001 tcttcgattg actggttgtt ttgtgatcag cctgaagcat atgttttgaa acgtgcaact
    36061 gcgttgagtg taccggcaga ttgtttttca cctaaagaat ttgattcaaa aaaagagtat
    36121 gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg atttgatcgt attagctgga
    36181 tatatgcgaa tcattggacc ggtattgctg aaaaattatg ataaaagaat catcaatatc
    36241 catccgtctc ttttacctgc cttccctggt ttgcatggca tacgtgatgc ctttgaagct
    36301 ggagtaaaag aaacgggtgt cacaattcat tatatcgacc aaggtgtaga tactggacca
    36361 atcatccggc aggaaaaagt gagaattgaa caggaggaca cgttcgattc tttagaagaa
    36421 aaaatccata gagtagagca tcgaatctat ccagaagtga ttagtgaaat tattgaaaat
    36481 ggaggctatc tgaaatgaca agagcattga tcagtgtatc agataaaact gggattgtag
    36541 cattcgccca aggtttgatt gaacaaggag tagaaatcat ttcaacagga ggaacaaagc
    36601 atgcgttgga taaagctggg atcaaaacgc tttcgatcga agaagtgaca gggtttcctg
    36661 aaatgatgga tggccgtgtc aaaacacttc atcctaaaat ccatggtggt cttttaggaa
    36721 gacgcgatct ccctagccat atggaggcaa tggaaacgga aaatatccaa ccaatcgact
    36781 ttgtttgtgt caatttatat cctttcaaag aaacgattat gaaaccagat gtcgaaatag
    36841 cagaagctat tgaaaacatt gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa
    36901 accatgaatt tgtcacctct gttgttgatc catctgacta tgaagaagta ctttcagaat
    36961 tacgtcagta tgggcatact tcactttctt tcagacaaaa actagcggca aaagtctttc
    37021 gtcatactgc ggcatatgat gcactgatcg ctcaatactt aacggattgg gtggaagaaa
    37081 aagaaccgga aaaattgaca cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa
    37141 atagccatca agccgctgcc ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag
    37201 cagcaaaaca actccatggg aaagaacttt cttataataa tatccgagat gcagatgctg
    37261 cgctacggat catgaaggaa tatgacgagc caacggtagt cgccttgaaa cacatgaatc
    37321 cttgtggtat tgggacagca tctacgattt tagaagcgtg ggaagcagcg tatgaagcag
    37381 atcctgtttc aatctttggt gggatcatcg ttttgaatcg agaagtagat ctgccaacag
    37441 cagaacagat gcataagctg tttctagaaa tcatcattgc cccttcttat acacaagaag
    37501 ctttggaatt actgcaaaaa aagaaaaaca tccgattact gacagtagac tttgcgcagg
    37561 aagaaaatca aaatcgagaa gaaacggtct cagtcttagg tggacttctt gtacaagatc
    37621 aagatctggc aatagaaaat gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc
    37681 cagcagaagc aaaagcatta gcgtttgctt ggagagctgt caaacatgtg aaatctaatg
    37741 cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg agccgggcag atgaatcgag
    37801 tcggttcagt aaaaattgcg attgaacaag cgcaagagaa aatggaaggt gcagttttag
    37861 ctagcgatgc ttattttcca atggatgatt cggttgaata tgcagccaaa catggtataa
    37921 aagcaatcat tcagccagga ggaagcatca aagaccaaga ttctattgat atggcgaata
    37981 aatacgatct agcaatggtc tttactgatg ttcggcattt tagacattag agaggagtcg
    38041 gataaatgaa cattttggtg atcggtgcag gtggtcgtga acatgcaata agtaaaaaac
    38101 ttttagccag tccgaaggtg gatacggtgt attgtgcgcc aggaaatccg gggatgacaa
    38161 aagatggtat caattgtgtc gctatcagtg agcgagatca ttcagcgtta atccattttg
    38221 ttaagaaaga ggctattgat tggagtatcg ttggtcctga agttcctttg ttaaatggga
    38281 tcgtcgatga ttttatggcg gaaggattga aaatcttcgg tccgacaaaa gcagcagctc
    38341 tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga tacttatcaa atcccgacag
    38401 ctaaatccag aagttttgaa tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg
    38461 cgccaatcgt agtgaaagcg gacggcttag cagctgggaa aggtgtagtc gtggctacca
    38521 cagtgaagga agcgctggat gcggcagaac aaatgttgaa ccagcatcga tttggggata
    38581 gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga agaattctcc cttttggcgt
    38641 ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca agatcataag cgggcatttg
    38701 ataacgataa agggccaaat acaggcggaa tgggtgctta ttcaccagta ccgcaaattt
    38761 caaaaaagat gattgaaaaa gctgtggaag aagttttaaa accagcggct ttgggaatga
    38821 aagaactagg acgctctttc acgggtgttt tatatgcggg tttgattgct acgaaggaag
    38881 gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt
    38941 ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt attagagaat cgtacagtag
    39001 agttaaagtg gcagcaagaa ggatacaatg taggcgttgt ggttgcagct gccggttacc
    39061 ccgaagaata tgagacagga atggttctac cagattttct ggaagaagag ctttctgttt
    39121 actatgctgg agtttcctca aataacggca aactagttgg agcaggagga cgtctttatt
    39181 tagtagaagc atacggtgag acgatcaaag aagcacagaa aaaaatttac accgcgttaa
    39241 tggataaaac gacagatgga acattttatc gtacagatat tggagaaaaa gctgccaata
    39301 gcgaactgat cgtgaagtaa atcagctgct tccatttata aaaaatacaa caaacataat
    39361 cggaagcgcc gattttagtg aagccttttc ataaagagtg tggtacactt ttgttgtgta
    39421 tataaataag gaggagttca gatgaaaaaa attcaattag gtacttctga tcagaaagca
    39481 gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt
    39541 gagacagctt atgaaaacgg cattgatttt tttgaccatg cagatattta tggaaacggt
    39601 gaatgtgaag aaatctttgc agatgctcta gcaaaaacat caatcaaacg agaagatctt
    39661 tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat
    39721 atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa tggattatgt ggatgcttta
    39781 cttcttcatc gtccagatac attggtagaa ccagaggaag tagctgaagc gtttgatgaa
    39841 ttggaacggt caggaaaagt tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc
    39901 gaattgctga aaaaatatgt tcgtcaacca ttagtagcta atcagctgca atttggcttg
    39961 aaacatactg gaatgatcga tcaagggatt catgtcaata tgacagatga agcaagtatc
    40021 gatcatgacg gtagtgtact tgattattcc cgattgcatg atatgacgat ccaagcatgg
    40081 tcaccatacc agtatggatt ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa
    40141 ttaaaccaaa cattagaaga actagcagct aaatatcaaa cgacaccaac tggcttggct
    40201 tctgcttgga ttttacgcca tccagccaat atgcaagtga tcgccggtac gatgaacgta
    40261 gggcgaatca aggaaatcgc tcaagcttca gaaattaata tgtctcggga agattggtac
    40321 gaattgtacc gtgctgctgg aaacatttta ccttaaagat tgtcataagc aggtcaaaag
    40381 ggctataaat taaaaagaca gaataacttg aaagctgttt tcagcttcga gatattctgt
    40441 ctttttttat tgcaaatgga aagagtatca ttgtacggat ttcgtttatt agttattttt
    40501 gtttttaagg aaaagctgtg tccgatattc ttttggagaa atcccttgtt ttttcttaaa
    40561 taaataactg aaatagttag ggtcattata accaatttca aaagcaatat ctgaaatacg
    40621 atagtctgat tgctgcaata atttttttgc taattcgatt ctttgttctg tcagataatc
    40681 gatgaatgtt tggttaaacg attgagaaaa aatcgtactg aaatgagagg gacttaatgc
    40741 aacttgttga gcgacccaag ttaaggacat atctggatcg gtaaaatttt ctttgataaa
    40801 atccaatgca tggttcaaaa ttggctgata tttgttcctt gaaggctgtg agggcagagc
    40861 agttaagaaa gaaatgaaca tataaactgt cgaacggtaa agcgagtcgt cggcgctgat
    40921 tgctaagcgt tgactggtgg atgtgcaatg aaccatttct tgcgggattt tcttttcttt
    40981 caggcagaca tgctggagta gcttagtcaa gacaaataaa cgatataatc gtgttcgagt
    41041 tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta gatacttcgt ttttcgataa
    41101 tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc
    41161 ttgaatcaaa tcctcatagc tgataatttg ttctttaagg gaactggcgg tttcaagtat
    41221 catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt ctttctgcga caggaccaat
    41281 ggcgacagaa aaattatttt tttgtccctt ttctaattca tggaccaacg tttgggctac
    41341 ctgataactg gtctcgatca cattttcttc aagagaatca actattaaaa atttgatgaa
    41401 tcgagaggaa acagcagaga agatcacttg ttgattctcc ctaaataaaa acttcaaata
    41461 atcactgaga tgagcgtaat cttcaaagtc tgtactgact tggtttgctg ctaataatac
    41521 ggtcattttt ttccctgcta aagagcgatt gaaagattca gcaagtttca atgtttcact
    41581 gatcgtaagt gagcctttga acaaaccgtt caagaaatga ttttttttca attcgaaaac
    41641 aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct aaagcagtta ctgctttttc
    41701 tattgcttgt ttcaattcgc tacttttaat cggtttaagc agataatcat ctgcctgtac
    41761 ctggattgct cctttcatat aatcgaaatc gtcgaatcca ctaataaata tgagacgagt
    41821 ccaaggaagt agtttccttg cttcttttga aaaagtcaac ccgtccatga aaggcatttg
    41881 gatatcagtc agaatgatgt cgggctgtaa gtcgataatt gaagacagtg ccatctcacc
    41941 atctccagct tcgccgacat aagtgattgg ataataagaa ccaaggacag ttatttgttc
    42001 ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata aaaactttat acatgtttgt
    42061 ttcctccaat tgttttggga gatagaggga tcatcagtgt gacagttgtt ccttctcggt
    42121 atttgctgtc aatcgtgata ggtttttgat catttccgta ataaagaagg agacgcttgt
    42181 ttacgttgta aagtccgtag ccgatactga aatctgaatc gatccctttg gctaactctt
    42241 tacgtatttc tgccagtttt tctgcaggga tgccaatccc gttgtctata actttgaatt
    42301 ggatcatctc atcaattttg catgcggtaa tacgaataaa tcctttgtga cggacgtact
    42361 tcgttccatg gtatacagca ttttcaatca atggctgcaa gatcattttt aaaatagaaa
    42421 caggaaaaag tgtttctggt atatcgatct tatagtcaag catatggccg tatcttattt
    42481 ttaagatgac tagataatca ttgatatggc taatttcttt ttcaagagga atccagtctt
    42541 gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt
    42601 cttcaaagca gtcttgttcg atcaaagaaa taatggcatc caaactgtta taaataaaat
    42661 ggggagtaat ctgagcttgc aatacacgaa cttcactttg tgctaaatgg tattgtttca
    42721 aagcgttctc ccgtaaagac tggtttagat cttccgccat tttattcatg ctagcggtaa
    42781 gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa
    42841 gttgttctga catctcgatc agctgattga tcggttcttg gacctttctc tctaaaaagc
    42901 gacgattctt tttcgcatag aaaaaaatgg tactaagaac tccaagttcc actattgtta
    42961 atagaatgag cgagcggaca agtgtcgtat tttttttgct tgctaagtcg atctccacac
    43021 gaacaaaatc ttgtaagata tctgacagca gttgagtgac agaatctacc tgattgatga
    43081 tctcttcatt ggtatccata aaatcttttg ccaagatatt attgatcagc tcgttttgat
    43141 aatcttccag tgtatcgatc gtacgtagcg atacatctaa cgtactaatt tctttaagtg
    43201 tatttgtatt atttttgatc tctcgaatat cattcctaag ttcttctagg atattttcct
    43261 cattgaactc atctacggtt acttggccag aaatcaagtc ccacatactt tccaaaactt
    43321 tttgatcaac cttcaaagca attgcatctg ctttttggat atttgcgatc gtctgttgat
    43381 aaatcaaaag ctgttgcgaa tataacagta tgctcagtag caaagggata acagcaaata
    43441 gaagcatggc tttgtttgct tgggcaaaaa tctgttttat gcttaaattt tttgtcatga
    43501 tacttctcca gtcaataatt tctggttggg atgttagcta tattctgctg ggaaaagaca
    43561 gtctcggaaa taaaaatatc tttaggtaat gtttttcctg tttgaatatt tccaaaataa
    43621 cgagtaacgg tattggctac ataccagccg acatcaggtt tacactcgac tacacaattg
    43681 acgattcctg agcgtagctt ctcgatcata tcttcttgtc catcaatagt tacaatgatc
    43741 aaatcttctc caggtttgat tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt
    43801 tcatcactat ggctgtataa tacatcgatt tcggataaat catttgtttc taaatatcgt
    43861 ttcatctggt cttttccttt catccgaata aaatcaccga caattcggtg aacaatagtc
    43921 atcggaggtt tatggtcgat ggttccatct ttttttaggg ttggtttacg attgattgat
    43981 tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt tctctgaacc agcaagttct
    44041 aggattctga tttcctgttt ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc
    44101 cgatttcctt ctgctttgaa actggggcca atgtgggtga cgaaaagctc gggatcattc
    44161 gtttgaatat gtctgtcgac aagtatgaca gggatccctg ctcgctttgc ttcttttaat
    44221 acagagtccc acccggattc ttgcaaagga gtaaaaatga tcatatccac tttataggca
    44281 atgaacgtcc gtatatcttg gatttgccgt tcctgattca tgtagccatt tcggtagaga
    44341 acttggtaat cttctttttc aagcgcagtg ataattgatt cagtatgctt tttccgccag
    44401 ctactctccg ttcctgattg cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta
    44461 ttaggtgtac accccatcat ttggtaagcg attacaacaa gggctaaaaa aaataaaaca
    44521 cgtttcactt taaatcactc catctggatt tgttagtact atcttatcag ataaaaggga
    44581 aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga aacttaaaaa aatccaagac
    44641 aaaaaaagta cacctaaaaa atttaaagta aatcccataa aaaaatcaat gttaacgctt
    44701 tctttattag ctatactcgg tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat
    44761 ttagaaagat tgttgggact atcgctattt tgaccgcaag cattctatta agtgcgtgtg
    44821 gcaattcagg atctgcagat gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat
    44881 cagcagaaag atggatcgct gatgggaaca acatggtctc ggaattagaa aaactgggtt
    44941 ataaaacaga tcttcaatat ggagaagata aagtagaaaa tcaagtagca caaatagaaa
    45001 atatgattac aaaaggggta gatacacttg tgattgcttc tatcgatggt tcggctttaa
    45061 ctgatgtgtt agctaaagca aaagaagcag atatcaaggt gatcgcctat gatcgtttac
    45121 tgatgaattc agaaaatgtc gattattacg cgacttttga taattttgga gtaggtgtat
    45181 cgcaagcagc ttatattgaa gagcacttag gattgaaaga aggaaaagga ccattcacga
    45241 ttgaattatt tggtggctcg cctgacgaca acaacgcact aatcaattac aacggagtga
    45301 tgtctgtttt acaaccatat atggacaacg gtcagttggt cgtgccatct ggacagacga
    45361 gtttcagtca aatcgctacg cttcgctggg atggttctac ggcgcaagca aggatggata
    45421 atctgttaag cgcaaattat actgaccaaa cattggatgc tgttttgtcg ccatatgatc
    45481 caataagttt aggaatcatt tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac
    45541 ctttacctgt gattactgga caagatgcga cagttgctgg cgttaaatcg atcattgccg
    45601 gggaacaaac acaaacgatc tttaaagaca cacgtatctt agcaaaaaat acgattgaaa
    45661 tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa tgatactgaa acttatgata
    45721 atggggtgaa aactgtccct acgtatttag caaatacagt atctgtcgat aaagataatt
    45781 atcaagcaga gctgatcgac accgattatt ataaagaatc cgatctgaaa aattaagagg
    45841 aaccaaacaa ggaactaaag agatctaagt cagagagagg ataagaatta tggcggattg
    45901 tattttaaaa atggcagata tcaccaaaaa attttctgga atccgagcgt tagattgcgt
    45961 gaatctgatg attgaacgtg gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa
    46021 atcaacattg atgaatgtct tgagcggctt gtatccatat ggctcctatg acggggaaat
    46081 cttttatgat ggaaagctgt gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat
    46141 cgtcatcatt catcaagaat tagcactaag tccttatctt tcaataaaag agaatatttt
    46201 tctcggaaat gaacaagcag aaagaggcgt gattgattgg gaactgacag aaaagaaaac
    46261 atatgaactg ctgaataaag tcggtttgtc tatcgacccg aatcttctag tatcccagat
    46321 tggcgtaggg caacagcaac tagttgaaat agctaaagca ttatcaaaat ccgtccgtct
    46381 gttgattttg gatgagccta ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag
    46441 attgatccga gagttgaaag agcaaggtgt aacctccatc attatttcac ataagctgaa
    46501 tgaaatcgtg gatatcgcag atcgaatcac cattttacgg gatggacaga cgattgagac
    46561 actagaaaat gaaatgatca gcgaagagca tattattcgc gggatggtgg gcagagattt
    46621 agttgatcgt tatcctgaaa gaaaaccgga tattggagaa gtttatttta aagtagaaga
    46681 ctggacggtc tatcaccctc ttgatcacga gcgaatcata aacgaccaca tcaacttgca
    46741 tttgaaaaaa ggagagattg taggaatagc tggactgatg ggagcgggca gaactgaatt
    46801 cgctatgagt ctatttggac attcttatgg aagcaagata tccggacgta tattaaagga
    46861 aggggcagag atatcagtta agaccgttcc ttcagccatt gcaaacggac tcgcttatgt
    46921 ctcggaggac cgaaaatctt taggactgaa tctgctgatg gatattcggg aaaacgtgac
    46981 gctgagcagt ttggataaaa taagtcaaaa aggagtatta gataaagaaa aagaagtaac
    47041 tgaggcagaa aatttccgga aaaagatgcg tataaaaaca aactccatcc atcaaattgt
    47101 cagtagtctt agtggtggga atcaacaaaa agtcgtttta tccaagtggc tgatgacaca
    47161 acccgatatt ctttttttgg atgagccaac ccgtgggata gatgttgggg caaaatatga
    47221 gatctacacg attatcgaag aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc
    47281 tgaacttcct gaaatattag ggatgtgtga tcgaatctat acgatgaacg aaggacgtat
    47341 tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa
    47401 agaagaggtg ggttaagttg gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac
    47461 taaatttgaa aagtagagca gctgatcttt ttggtcgcta cagcatggtg atcattttag
    47521 cgggactact tattgcgttt caattgatga cggatggtat tttctggcgt ccattgaata
    47581 taactaacct tgttctgcaa aatagccata tattagtact agctgcaggg atgttattag
    47641 ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa
    47701 ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc
    47761 ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg ggtagcttat gcagggatac
    47821 cggcgtttat cgtgacttta gcaggattgc tgatgtttcg tggactgaca caagttgttc
    47881 taggaggcca atctctagca ccttttccag ctgttttcca aaaaatgtct acgggttatt
    47941 taccagactt aagcgaagga agcagccttc acttcttgac attgatttta ggaatgatca
    48001 tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat
    48061 ttgaagtgga atctccaaca gctttcttgg taaaggcggt atttacagca gtcgttgtac
    48121 taggaataac ttatatcttt gcctcttatc aagggtatcc gattattttg atcattcttg
    48181 gtttgatcgt tgcagcatat gggtttttga caaataaaac agtagcaggt cgacagattt
    48241 atgcgacggg cggaaatcga aaagcggctg aactatcagg gatcaaaaca aagcgaatca
    48301 cgttctgggt attcgtcaac atgggagtca tggcagcttt agctggatta gttttagctg
    48361 ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat ggaactggat gcaatggcgg
    48421 cagtatattt tggtggtgct tcgacttccg gcggcattgg aacaatcatg ggtgcgattg
    48481 tcggtggctt ggtcatgggt gtattgaaca atggcatgtc gattttaggc gtcggggtag
    48541 actggcagca ggcaatcaaa ggattgatct tgcttttagc cgttgtgcta gacttgtata
    48601 ataagaaaaa acgaacaacg taggagaacg ccggttattc agaaaaaaga ctgtgacaag
    48661 acatttgtca cagtcttttt ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac
    48721 taaaaataat atatgttttt ttaccattcg tataaattct ctttttgctc ttgatttttt
    48781 tgcttaattg agttaaatta catagggcgc taaaaaaagc cttgattaaa ttaataatgt
    48841 tcgtgttttg ctggatgtga acatcaggag gataatgtaa tgaaagtatt tgattacgaa
    48901 gatattcaat taattcctaa taaatgtatc gtaaatagcc gttctgaatg tgatacaacg
    48961 gtcacattag gtaaacatac attcaaaatg ccagtagtgc ctgccaacat gcagacgatt
    49021 attgatgaaa caatcgcaga attcttagcg gaaaatggtt atttctatat catgcatcgg
    49081 tttgatgaag cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg attgattacc
    49141 tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta tagagaagtt ggcagaagaa
    49201 tcgctaaatc ctgattatat cactatcgac atcgcgcatg gacatgctaa ctcagttatt
    49261 gatatgattc agcatattaa aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt
    49321 ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg
    49381 attggtccag gaaaagtatg tattacaaaa atcaagacag gctttgggac tggaggctgg
    49441 caactggcag ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt
    49501 gggatcagaa cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg
    49561 attggttcat tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga
    49621 atcgtgtata aagaatactt tggttctgct tcagaattcc aaaaaggcga aaaacgaaat
    49681 gttgaaggaa agaaaatctg gatccaacac aaaggatctt tgaaagatac actagtggaa
    49741 atgcaacaag accttcaatc atctatttct tatgctggag gaagagatct ggaagccatt
    49801 cgaaaagttg actatgtcat cgtgaaaaat tcgattttta atggagacac aatctaaaaa
    49861 ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc
    49921 aaacaaaaat agttctttat atgttttgtt tggctggaat tattatcgca gatacaagct
    49981 tttgaacatc attattcaga ataagggact gtgataagat ttttgtcaca gtcccttatg
    50041 ttttgcaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag
    50101 acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga ccgatttatc
    50161 gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc
    50221 aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc accgttgtcc
    50281 atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc agcggatggt
    50341 ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg cggcaaatga
    50401 tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa gcaaagcaga
    50461 tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag cttttgccga
    50521 attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc
    50581 ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat
    50641 tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca atctttttga
    50701 ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct tctttttttt
    50761 accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg atggatgaag
    50821 aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg aaagcagtgc
    50881 ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa
    50941 agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat gtatttacgg
    51001 atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat ccggataatg
    51061 tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc ggttgggtga
    51121 taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg gagttttcga
    51181 tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt gatttgaaaa
    51241 tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct tggggagatt
    51301 cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg attacttggg
    51361 atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt cttggaattg
    51421 caccaagtct actttttggg caagatataa atggaatcag aatcaattac agtaggctgg
    51481 atgaaaacca aataccgttg ttttattcaa ag
//
LOCUS       DADXTG010000005        45922 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.005, whole genome shotgun sequence.
ACCESSION   DADXTG010000005 DADXTG010000000
VERSION     DADXTG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.005"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..256)
                     /locus_tag="IWI57_000213"
                     /pseudo
     CDS             complement(1..256)
                     /locus_tag="IWI57_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(510..2145)
                     /locus_tag="IWI57_000214"
                     /pseudo
     CDS             complement(510..2145)
                     /locus_tag="IWI57_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2468..2947)
                     /gene="rlmH"
                     /locus_tag="IWI57_000215"
     CDS             complement(2468..2947)
                     /gene="rlmH"
                     /locus_tag="IWI57_000215"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBC2448385.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3375..4664
                     /locus_tag="IWI57_000216"
     CDS             3375..4664
                     /locus_tag="IWI57_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBC2448386.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4703..5308)
                     /locus_tag="IWI57_000217"
     CDS             complement(4703..5308)
                     /locus_tag="IWI57_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBC2448387.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5513..5977)
                     /locus_tag="IWI57_000218"
     CDS             complement(5513..5977)
                     /locus_tag="IWI57_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBC2448388.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5999..6319)
                     /locus_tag="IWI57_000219"
     CDS             complement(5999..6319)
                     /locus_tag="IWI57_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBC2448389.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6463..7542)
                     /gene="pepA"
                     /locus_tag="IWI57_000220"
     CDS             complement(6463..7542)
                     /gene="pepA"
                     /locus_tag="IWI57_000220"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBC2448390.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7742..8092
                     /locus_tag="IWI57_000221"
     CDS             7742..8092
                     /locus_tag="IWI57_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBC2448391.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8315..9262
                     /locus_tag="IWI57_000222"
     CDS             8315..9262
                     /locus_tag="IWI57_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2448392.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9319..10737)
                     /gene="pepV"
                     /locus_tag="IWI57_000223"
     CDS             complement(9319..10737)
                     /gene="pepV"
                     /locus_tag="IWI57_000223"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBC2448393.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10886..11731
                     /locus_tag="IWI57_000224"
     CDS             10886..11731
                     /locus_tag="IWI57_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBC2448394.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11735..12175
                     /locus_tag="IWI57_000225"
     CDS             11735..12175
                     /locus_tag="IWI57_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBC2448395.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12225..12419
                     /locus_tag="IWI57_000226"
     CDS             12225..12419
                     /locus_tag="IWI57_000226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448396.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12555..13241
                     /locus_tag="IWI57_000227"
     CDS             12555..13241
                     /locus_tag="IWI57_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2448397.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13649..14338)
                     /locus_tag="IWI57_000228"
     CDS             complement(13649..14338)
                     /locus_tag="IWI57_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448398.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14313..16442)
                     /locus_tag="IWI57_000229"
     CDS             complement(14313..16442)
                     /locus_tag="IWI57_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBC2448399.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16490..17827)
                     /locus_tag="IWI57_000230"
     CDS             complement(16490..17827)
                     /locus_tag="IWI57_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBC2448400.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18079..18438)
                     /gene="rplT"
                     /locus_tag="IWI57_000231"
     CDS             complement(18079..18438)
                     /gene="rplT"
                     /locus_tag="IWI57_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBC2448401.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18491..18691)
                     /gene="rpmI"
                     /locus_tag="IWI57_000232"
     CDS             complement(18491..18691)
                     /gene="rpmI"
                     /locus_tag="IWI57_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBC2448402.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18763..19287)
                     /gene="infC"
                     /locus_tag="IWI57_000233"
     CDS             complement(18763..19287)
                     /gene="infC"
                     /locus_tag="IWI57_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBC2448403.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19331..19456)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19607..20239
                     /locus_tag="IWI57_000234"
     CDS             19607..20239
                     /locus_tag="IWI57_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBC2448404.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20241..21146)
                     /locus_tag="IWI57_000235"
     CDS             complement(20241..21146)
                     /locus_tag="IWI57_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBC2448405.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21231..21890)
                     /locus_tag="IWI57_000236"
     CDS             complement(21231..21890)
                     /locus_tag="IWI57_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBC2448406.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22206..23150
                     /gene="mvk"
                     /locus_tag="IWI57_000237"
     CDS             22206..23150
                     /gene="mvk"
                     /locus_tag="IWI57_000237"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBC2448407.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23143..24120
                     /gene="mvaD"
                     /locus_tag="IWI57_000238"
     CDS             23143..24120
                     /gene="mvaD"
                     /locus_tag="IWI57_000238"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBC2448408.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24133..25218
                     /locus_tag="IWI57_000239"
     CDS             24133..25218
                     /locus_tag="IWI57_000239"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBC2448409.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25215..26279
                     /locus_tag="IWI57_000240"
     CDS             25215..26279
                     /locus_tag="IWI57_000240"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBC2448410.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26528..27331)
                     /locus_tag="IWI57_000241"
     CDS             complement(26528..27331)
                     /locus_tag="IWI57_000241"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBC2448411.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27351..28193)
                     /gene="kduI"
                     /locus_tag="IWI57_000242"
     CDS             complement(27351..28193)
                     /gene="kduI"
                     /locus_tag="IWI57_000242"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBC2448412.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28209..29216)
                     /locus_tag="IWI57_000243"
     CDS             complement(28209..29216)
                     /locus_tag="IWI57_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBC2448413.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29223..29867)
                     /locus_tag="IWI57_000244"
     CDS             complement(29223..29867)
                     /locus_tag="IWI57_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBC2448414.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29967..30725)
                     /locus_tag="IWI57_000245"
     CDS             complement(29967..30725)
                     /locus_tag="IWI57_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBC2448415.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30852..32003)
                     /gene="fucO"
                     /locus_tag="IWI57_000246"
     CDS             complement(30852..32003)
                     /gene="fucO"
                     /locus_tag="IWI57_000246"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBC2448416.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(32085..32396)
                     /gene="rhaM"
                     /locus_tag="IWI57_000247"
     CDS             complement(32085..32396)
                     /gene="rhaM"
                     /locus_tag="IWI57_000247"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBC2448417.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32398..33243)
                     /gene="rhaD"
                     /locus_tag="IWI57_000248"
     CDS             complement(32398..33243)
                     /gene="rhaD"
                     /locus_tag="IWI57_000248"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBC2448418.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33257..34513)
                     /gene="rhaA"
                     /locus_tag="IWI57_000249"
     CDS             complement(33257..34513)
                     /gene="rhaA"
                     /locus_tag="IWI57_000249"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBC2448419.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34510..35970)
                     /gene="rhaB"
                     /locus_tag="IWI57_000250"
     CDS             complement(34510..35970)
                     /gene="rhaB"
                     /locus_tag="IWI57_000250"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBC2448420.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36123..36992
                     /locus_tag="IWI57_000251"
     CDS             36123..36992
                     /locus_tag="IWI57_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBC2448421.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(37118..38017)
                     /locus_tag="IWI57_000252"
     CDS             complement(37118..38017)
                     /locus_tag="IWI57_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2448422.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38182..39006)
                     /locus_tag="IWI57_000253"
     CDS             complement(38182..39006)
                     /locus_tag="IWI57_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBC2448423.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(39017..40312)
                     /locus_tag="IWI57_000254"
     CDS             complement(39017..40312)
                     /locus_tag="IWI57_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBC2448424.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40328..40807)
                     /locus_tag="IWI57_000255"
     CDS             complement(40328..40807)
                     /locus_tag="IWI57_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBC2448425.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40813..41841)
                     /locus_tag="IWI57_000256"
     CDS             complement(40813..41841)
                     /locus_tag="IWI57_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBC2448426.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41858..42964)
                     /locus_tag="IWI57_000257"
     CDS             complement(41858..42964)
                     /locus_tag="IWI57_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBC2448427.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43193..45790)
                     /gene="adhE"
                     /locus_tag="IWI57_000258"
                     /gene_synonym="adhC"
     CDS             complement(43193..45790)
                     /gene="adhE"
                     /locus_tag="IWI57_000258"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBC2448428.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13292 a         9752 c         8311 g        14567 t
ORIGIN      
        1 gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg atgtttggct ttatatcctt
       61 cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca cctggcatat
      121 attgcagatt agaagcatgt aattttttct cagaaacaaa ccactcattg gaaaagacat
      181 gaatcaaatc atcacttact tgtgtaaaat agcgacgttt aatgacaaag atatcttcat
      241 tgctatctag tgtaccttca tttctttgtg cttaaattca aagataattt tctcaataaa
      301 gatttttgat tccagtaaga ttcctttact ccttttacaa catagcgttg tcctttgttt
      361 tgaaacatct tcttcccctc tttgccatta aaaatagccc aattctctat aaagaagatt
      421 aagcttcatt attataatat aaagtttaaa tttggtttat tacttacttt ttcttattct
      481 ctagtaagct aatattgaaa aaaatgtaat cagatcatta tttttatatg ttgtaacttc
      541 ctgataagaa aactaattaa aagaaagagg taactacata aactaattaa aaaagctaac
      601 ttcatagttg gaggataaat ggaagaaact tcaatgattg tttctccttg tgttccttca
      661 atcgacacta gtcctattat caatctctct tttttgtcca tttacttttg cgactgttcc
      721 ccaataatca aatacaggaa gcgttatttt ttgtccttta gattcatgta ctgtatagac
      781 tattttcgaa tccatcactt gataaggcat agtatgccat tccttgccaa ttcttacttc
      841 atgattctgc aaatgttctt tgtattgtaa tgcttccgtg ggtgcatagt cataaagtcc
      901 tccgggaagg agtgtcgtgt aacgaataag attttcttga ttcattacca agtgatctct
      961 ctgagtgaca actttatgtt catagtttgc ttttgttgca gcagatacag ccaacagtaa
     1021 taaaacaatt gccgttatat taactacttt tctttgctta gctgatgtgc tcttcatgaa
     1081 aacgaacgat aaaatcaacg agccaaacag tacttgaaag cctaagattc gccaaggaaa
     1141 ttgaatcatt tgaatgggtg tattttgtaa taaagaccat ggaaaaaatt tggtggataa
     1201 gagccaagag actaccgcac taaggtaaat cactttttct ttgcgattaa atcctttaaa
     1261 aaagaagagt ggacagatca aagaaataat accaatgatt cctaccgtaa aggttgtcag
     1321 gtcattattc atcgcaccaa tcaaactatc cgagacattt aatgcttggt gttgtaaggt
     1381 cgttttatct ggctcattaa tcgattgaac catgatttgt cggaacatcg gataccaaaa
     1441 gtatgctgtt aaaagaatcg tcattcctac tgcctttgac aactccacaa tcaaccggat
     1501 gctttctttt tttcttgttt cacttactac aaagccgcct aagactagta agccaacggc
     1561 tattacactc ataaaagtgg aaagaacatg tgtataaatg agtaagctca tactgactgc
     1621 taatggaatt gcacatggct ttttctcata aatcagtgcg taagcataat acataactat
     1681 cggtataacc gtaagtgcaa tcgcttcagc aatagcagaa cgatgataaa tatcaatcgt
     1741 acggtaccca taaaatgtat ataaacagct aaatataaaa gccgcatcga ttctttttaa
     1801 aaattttagt ccataatgaa acatcagcat gatagtacaa acattcaata gccaaacata
     1861 taagatataa ctaacgatta aattattact tatccaataa aaaatgactg ccggaaaaaa
     1921 ggtcaagtag ggataaaaat aattcactcc gctcccataa tgattaaacg tcgtaaaatt
     1981 gattggacca gaaaaaattg aagataaccc tttgactcta gcaaaatgga aggcaatatc
     2041 ccccgatgta gaaatagtat gaacatttct aaaataattg attgaataaa gtccagcaat
     2101 aattgctagg agaatggaca atattatttt tttctttact tgcattactt aattatatac
     2161 tcctttcatg gattgcttta tctagtataa cttaaatttt aaataaatcg ttatttattt
     2221 ttactctcac ttaaaaaagc actaaaatta taagagaggg aggtaatggt ctgaacacca
     2281 agaattgttc caacatcgaa ttgcttaact aatgagttca aagcttaatt atgtggctct
     2341 tatatttacc gagcgtggat tgcgatctct ttagcgaaac aaacatcttt cataaaaaaa
     2401 acagccatat ccgctttctt ggatatgact atttactact actttactat ctttcattct
     2461 tcttgtctca cttatgataa ggcgcccccg cattgatccg gaaggcacgg tagatttgct
     2521 cgacaaggat cagacgcatc aattgatgtg gatacgtcat ttttccaaaa gagatctgag
     2581 cattgcttct tttcatgacg gcttcgctga gtcctaaaga gccgccgata acaaaaacaa
     2641 ggtggctttt tccttggata cctaattggt cgatttgttt agcaaaagat tcacttgtag
     2701 gatttttccc ttcaattgct aaggcataga cgtattcttg ttctttgatt ttcgctaaga
     2761 tccgttcccc ttctttttct tttacttgtc tcatttgcgc ttcgcttaaa ttctccgggg
     2821 ctttttcgtc aggaacttcg atcaattcga tttttgcata ggcattcagc cgtttgacat
     2881 actcattgat accttggatc aggtattttt cttttaattt tcctactgag ataattttga
     2941 tattcatgtt ttctctcctt tggtcttcat gctcactttg tttattataa tcaatttttt
     3001 cgtctatatg tttattttgg ctatttttgt taaaaagatt tccccaacag ttatgcacaa
     3061 catccacagg tttatccaca gaaaacagtg ttattttacc ttatttcatc acattcatta
     3121 ggcaaacata cgttttataa gaatttatcc actttataca caggctgtgg atatgtttta
     3181 tcccttattt ttccaatcct atccgcgtag ttttacacag tatctgtgga aaaagaaaat
     3241 caaatatttt catctgaaaa caatgatttt tcctaaagtt tttcataaaa agttcacagt
     3301 ctcaaagtag actaaagtca acaaataaga ctatactaac agtagataaa tgaatgtaat
     3361 tggaggagga acacatggat agaaaaaatg tgacaccaaa gatgaaaaaa agtaaaaata
     3421 atagcctctg gcgtaagtta ggtctggggc ttgttggagg gatcgttgga ggactactga
     3481 cagctggcat tttttatgca gcgatgggca ctggcaacaa tagttctact gctacaagcg
     3541 gcaatcagaa ttctgccggt gaaacagttg tggaaaatgt caaagtcaat gtagattctg
     3601 atatcaccaa agcggtagat aaagttcaag acgctgtcgt ttctgttatc aacttacaaa
     3661 gtcaaaacca atcaagcggt tttggcggac tattcgggca gcaagaagaa agtagcagct
     3721 cagatgatag taatttggaa gcttatagtg aaggaagcgg cgtgatctac aaaaaagacg
     3781 ggaatactgc ttatgtagtc acgaataatc acgtagtaga tggccagcaa ggtcttgaag
     3841 tcttgatgaa agacggtacg aaggtaaaag ctgaattagt tggaacagat gcttattcag
     3901 atttagcggt gttgaagatc aattcagaca aagtcgaaac agttgcctct tttggtgact
     3961 caagtgctct gaaggttggt gagcctgcga ttgcgattgg ttccccacta ggatctgaat
     4021 atgcaaattc tgtcacttca ggaatcattt cttccttgaa ccgacaagtg acaagtacga
     4081 atgaatcgaa cgaaacagtc aatatcaatg cgatccaaac agatgcagca atcaaccctg
     4141 gaaattctgg tggtccttta gttaatatcg aaggacaagt tatcggtatc aactcaagta
     4201 agatcgcaag tacttctgaa tctacttcta atgtaagcgt agaaggaatg ggatttgcta
     4261 ttccaagtaa cgatgtagtg aatatcatca atcaactgga aaaagacgga aaagtgacac
     4321 gtccagctct aggtatcaca atggtcgact tatctgccgt ttctactcag caacaagaac
     4381 aaatcttgaa gattccttca tctgtaacta acggtgtcat cgtgacaaat gtccaaacag
     4441 ctacaccggc tgaaaaagct ggattgaaac aatacgacgt cattactaag atcgatgaca
     4501 aagaagtttc ttctggtgta gaacttcaat ctgctttgta taagaaaaaa gtgggagatt
     4561 ctgtcaaaat cacttactat gaaggctcga aagaaaaaac aactactgtt gaattgacga
     4621 tcgatcaatc ggcattaaaa caaaatcaaa atagtgaaaa ttaataaaaa agaggttgtg
     4681 acaaagactt gtcacaacct ctttattttg gaaatgcttc tccgactacg gcatcctcag
     4741 gcaatactaa gattcctttt tttgctggag catttggcaa atgcaattca cgagcggaac
     4801 agatcattcc atagctatct acccctcgca aagttcctgg ccagatcatc attccatctg
     4861 gcatcatagc tcctggtttt gcaacgacaa ctttttgtcc agcttcgata ttgggtgcac
     4921 cgcaaacgat ttgcaatact gtaccattgt ctacttctgt ctcagtaatc gataaatggt
     4981 cagaatcagg atgtgggacg catgttttta cgtacccgac aacgatcttc ggttcatgat
     5041 cagcttcaag ctgttcttcg aatcctgctt ctttgatcaa tcgattcaat ttcgctaatt
     5101 cctcatctgt taagcttact tggccgttcc cttcgattgt tagatgatcg gagatatgga
     5161 aaaagttcca agcaacaact tgaccttgtt catctttgat ccgagcaaca ttccatttgc
     5221 gttcaacagt gttttctgtt cctttatcgt cggctataat aaccatcaag gtgtctccga
     5281 cgtgtgcttt gttataagca aaaatcatgt tctttcctcc attatccttt gctgagattt
     5341 atcttaacaa aaaattgcat tcactgcatg aaattttttt catgctttat ttccgaagaa
     5401 gcctcttctg gaacaccatt tattcgtaac aagggactgt gacaagactt ttgtcacagt
     5461 ccctttactg ttttagttaa ttgattaaaa aggtcgtaca agttccactt gcttaattac
     5521 ttggctgttc ttcttcgtca gaaacgatca agacatcaca aggtgaatga cgtatcacgt
     5581 agctggctac acttcctgtc ataaagcgtt cgactgcatt tagacctgac tgcccaacca
     5641 tgattagatc gatctcatat ttttttggca atttgactgc catggattca cgaacagaac
     5701 catatgcaat aattccttct atatttgtgt aatcaacaga tttcccatat tctttacatt
     5761 cttcgatcat ttccttggct gtttcagttt gctgatcgac tagattttga tcaataggtg
     5821 catagcccat aaagttataa aattgctgat cgattacgtt tgctacatat actttcccat
     5881 tattacggcg agccacttct accgcttttt tgaaagcagc aagtgcttgt tcactaccgt
     5941 cgatacctac taaaatattc ttatattctt gattcatctt gacccctcct aacagatgtc
     6001 agccttttgt tacactttct aggaacgatt cgatttcctg ttttgtttta cgatctttat
     6061 tcactagacg acctaattct tttccgtctt ggatcacgac aaaacttggg ataccaaaaa
     6121 tgttccattc agcggctaca tctatgtact tgtcacggtc tacttcaata aattgccaat
     6181 cagaaaagtc attttcaatt tcaggcattt gcggtttgat aaaacggcag tctccgcacc
     6241 agccggcagt aaagaaaaat acactctttc cagtttctac gtagcctgct aattcttcta
     6301 aactatttgg gataatcatt ctttgtcctc tactttctgt ttatgtattt ttcaattaaa
     6361 ttatacacct ttctttcgaa ctaacaaaca ttgatcctct ttttataaaa aaaatctggc
     6421 aaaaagcaat cttttgccag attttatagt ttcactatcg cattattttc cgtatacgat
     6481 cgtatttact gttgatttat ccaaaccttt cagtgtttga atcaacattt ctcgtgcagc
     6541 ttcaaaatcg cgaatactga acatcgtttg atgcgtatgg atataacgtc ccactacgcc
     6601 aatcactgta cttgggattc cttcgttttg tgtatgagca gcacctgcat ctgtaccgcc
     6661 ttttgaaaca aagtattgat aagggatatt gtttgtttca gcgatatcca gcaagtactc
     6721 gcgcaaacga ggaagcatga tcaaacctgg atcttggata cgcatcaatg taccttctcc
     6781 taaatgtccg aatgtgccat ttttcgtcac tgtatcatct gctgcagagc agtctactgc
     6841 aaagaacaga tctggcttga atttattgac agaagcttta gaaccacgca agcctacttc
     6901 ttcctggaca ttcgcgcctg caatcaatgt atgacctaat gtttcatttt gcaacgcttc
     6961 taaagcttct aataccattg tacagccata gcggttgtcc catgatttac tgataatatt
     7021 ctttccgttg gctgttttga tcgtttcagc gtaaggaacg attgtgtctc ctggaaggac
     7081 accgtaactc attgcctctt ctcttgattc aaacccagca tcgaacaaaa tatcggttac
     7141 ttctactgat ttttgtccgc ttgttcctcg caataaatgt gggggaacgg aagaagaaat
     7201 acatggataa ttcttaccag tagaggtttt aagtgtaaaa cgctgagcag aaacaacgta
     7261 aggattccag ccgcctagag gtacgacttt aaaaagcccg ttttcttgga tttgtgtcag
     7321 catgaatccg acttcatcca tatgtgctgc gaccattaca cggggtgcat ccgcttcttc
     7381 atgatggcgc agaccaaaaa tgccgcccaa accatctaac tgtaactcat ctaccagcgg
     7441 ttccatatga ctttccatat aagcgcgaac atcttgttca aaaccgcttg ttccttgtaa
     7501 ttctgttaac tctttgattc gttgaaatgt tttttcttcc atatgctatt cgtctaaatt
     7561 tcagactaaa ctcctctcag acttatattg atctgcggat aaaatatgat tttttctgca
     7621 gaccgatcgt atttattata gcttattttt tctggtagcg gttatttatt tcaaagaaaa
     7681 tatgagagtg tggtaaaata agcacaagga gtaacaagtg agaagaagga ggtatgtcca
     7741 catgtatgaa gaaagcgaac taagctattt taaaggcggg ttagcagttg gaatcggtct
     7801 aggattattg agcggggtag cttcaacgat ttggtatcat cgcaaaaaag tattgtcagc
     7861 agaccaagta cttgaaaata tcaagtcatc ttttctaaaa gaaggtgcaa tcgaaggttc
     7921 ttggatctct tttgaaaagg aacctattag gaaattcgcc gttcggtcga gtgcttatcg
     7981 gggaggtctt tctcgaatag aagatggcga aactgtttat tatgaatttt tagcagatgc
     8041 tcatacagga accgtattag aaatttcaag aaaaaaaggt gcaacagatt aatacgtcaa
     8101 aaaagtgaga gaacgaatac gcacccacta ttcaaaagag aggtatcatg tccttctttt
     8161 gtagtgggtg gttttttaga tagagctttc caaacatata ttccagtatt ttctttttaa
     8221 ataaatgata tttagcggga aaaactagca tttcctttga ttatgaggta tttttaattt
     8281 agcttttgtt ttctatagtt aaggagagtg aaaaatggaa caacaagaaa aaattgctgt
     8341 tctgattcct tgttacaatg aggaagcaac aatttcaaca gtcattgcag actttaaacg
     8401 agaattacca gaagcagata tttatgtcta tgataataat tcgaccgatc gaacatacga
     8461 attggccgtt caaggtggag caatcgtaaa aaaagaacct cgtcaaggaa aaggaaatgt
     8521 gatccgccaa atgttctttg atatcgatgc cgactattat ttaatggtag atggcgatga
     8581 tacttatcct gcagaagctg tacatggttt gctcgagaaa ttacgttctg gtgaagcaga
     8641 tatggtcatt ggtgatcgcc tatcaaatgg aacctacttt gatgagaata agagaccttt
     8701 ccacgatttt ggaaataacc ttgttagaaa tacaattact agaatgtata aaacaaaaat
     8761 tcttgatgtc atgaccggct atcgaggatt caatcgtatc tttgtcaaaa gttttcctat
     8821 tatgagttct ggttttcaaa tcgaaacgga attgacgatc catgctttag ataaaaaatt
     8881 caagttcgtt gaaatcccaa tcgattatcg tgatcgtcct gaaggcagcg aatcaaaatt
     8941 gaatacattc tctgacggat tcaaagtgat catgatgatt attaaaatgt ttaaagatta
     9001 taagcctttg cttttctttg ggatttggac catcttcttc tttttatttg gattggtcac
     9061 ggggattcct gtgattcgcg aatatatgct gacaagcttt attacacgca tcccttctgc
     9121 gattttaagt actggattga tgactttagc acttctttca ttagtaacag ggttgatttt
     9181 ggatacagtc gtcacgaacg ctaagaaaga atatgaattg aatctatatc atgtttatca
     9241 tgaataccac agtagaaagt aaaaaaagga tcaagactgc aaaaataatt tgcaattctt
     9301 gatcctcttt ttaattaatt atttcgccaa acgatagatg gcatctgcat agattgctgt
     9361 tgctctgaac aagtcatcaa gactcataaa ttcgtttgct tgatgcattg tatcagtata
     9421 tcctgggaac atcgctccat aagcaacgcc acgtttcaac aaacgtccgt aagttccgcc
     9481 accgatgatt tgttcgtagc ctttttctcc agtgtggtct tcatagactt gcaataatgt
     9541 agccaccaat ggatcatcca ttggtacgta atgtggttcc attacgcgag cacctcgagt
     9601 aactgttgcg ccttcttgcc ccactgtttg ttcgatgtca ctttgcaatc cgtctactgt
     9661 tacacctttt ggaaaacgga aattcatatt gatgaagttc gcttcctctg taccattttc
     9721 ttcaaaagca aatagtccag cattcattgt caatttgccc attttttcat cttcataagc
     9781 gactcctagt ttttctccat aaaaatcttc atggacataa gcagctgcta catggataaa
     9841 ttgtttagca gatcctagga attcatagtt atctaagaac aatgccaaga aagaacctgc
     9901 attgatacca gattgtgggc ttgcaccatg tgcgccttta ccaactaatt cgatttttac
     9961 atacgtattg tccgcttcaa tcgttccgct tactggattt gcttcgatga attggtaaaa
    10021 tgcttcttcc atcgcgatag ctgcatctgc acttggtact tgaagtgctg cagttgctgt
    10081 tcctggcacc atattttcgc gaagtccgga aacaaaagat ttcaagacat aatctcctgc
    10141 attgtcgcct ttgaagcgta aagctaaaga aacatttcct ttttcaccat tgatgattgg
    10201 gaattcagca tctggtgaga aaccaaaatc tggttcttct tcatgttcga aataataagc
    10261 catatcgccc cagccgcttt cttcatcgct gccgactacg aagcgaactt tttttgatag
    10321 tttcaagtca agttctttaa tgatcttcat cgcatagtaa gctgccatac ttggaccttt
    10381 gtcatcgctt gatccgcgtg caaagatctt tccatccttg attactggtt cataaggatc
    10441 tgtgtcccag ccatctcctg caggaacgac atccatgtgt ccaaatatcc ctagcgtttc
    10501 gtctccttcg ccaagatcaa tatgccctgc atagttatcg acatttttta ctttaaatcc
    10561 atcacgttcg ccataagcta acatatgttt caatgcgtcg cgaggtccag gtccaaatgg
    10621 tgcatctggt gttacttttg agtcatcgcg ctcgctgttg acacgtaaaa gatttttcaa
    10681 gtcttcaagc aagtcttctt tacgtgcttc tacttctttt tgccaatcaa ttgtcataaa
    10741 gttaatcctc cacttattta agtcttcgtg ttcatgatac cacaaattac agaaaaaaat
    10801 agttcaaaaa atctgacaat tccttttctt atctgttttt ctctaaaaaa aatatacgct
    10861 ataatgagta aaaggaggtt tttagatgct gataaacgaa caactagtga aaagagtcat
    10921 cacacctcgt ccaaaaattt cacataaagg aacatttgga cgtatcgttt tgattggcgg
    10981 caatgaacaa tatggcggag caatcattat gagtgcttta gctgccgtac acagcggagc
    11041 gggtctcacg actgtagtaa ctgatgaaaa aaatcggagt gctcttcaca gtcatctgcc
    11101 agaagcaatg attgtagttt ggacggatac tgaccagttt ctttccgtaa taaagcaagc
    11161 agatgtactc ttgattggtc ccggattggg tacttcttct aagagtttgg aacgtttcaa
    11221 acaagtacta gaaaaccaat caaaagaaca atggctggtg attgatggct ctgctttaag
    11281 tttacttgct gctaatcatc ttttgctacc ctatcccgaa caaactgtac ttacacctca
    11341 tcaaatggag tggcaacggt tgagttctct accgatacaa gaacaacggg ataaagaaaa
    11401 caaaaagaag caacaggaaa tcggtgctgt gctcgttttg aaaagtcatg agacaaaaat
    11461 ttatggtcac cagcacttct acaccaatcc tttaggttct gctgctatgg caacgggcgg
    11521 catgggtgac actttagctg ggatgatcac aggattttta gcccagtttc ctcaaaatga
    11581 ccaaacgatt gcagcagcta tttatcttca tagtttcatt ggagaccagc tggcaaaaga
    11641 acaatatatt gttttgccga cacaaatcag cgaagcactt ccgaaatgga tgaattattt
    11701 ttgtaaaaaa tcaatggaaa acgaggcatg agtcatggag aaatttacag gttatgtgcg
    11761 agaacctttt tattatgaaa cagatcaaat ggggattatt catcacagca attatatccg
    11821 ctggtttgaa gaggctcggg tagccttatt ggtacatcta gggtttccct ataaaaaaat
    11881 cgaagaatct gggatcatca tccccgttct ggaagtaaat tgcagctata aagaaatgat
    11941 ccattacgga gaaaaagtga gcatccatcc aactatccaa aagtatacgg ggacacggtt
    12001 ggattttagc tacgaaattg tgggaactga agacaaaaaa ttaaaaacta ctggcagtag
    12061 taaacactgt tttttacttt ctgattcgca acgattagtg aagctgagca aagtcaatcc
    12121 ggagttagat cagttattcc aagcgtacgc ttctttggat gatgtaaatt cttaataaag
    12181 accgtctaag catttttctt gtattgtgtc gagtagttcg tataatgaaa agaaaaaagc
    12241 aggtgatccc catgtatgtc gttaaagtaa tgcacggtta catcgataaa actggctgtc
    12301 gcactcggga aaaaaactta gacaatctgc tcattttcaa agacaaaaaa gaatccgaag
    12361 cttttgctaa aagaatcggc ggtcgtgtca aaccgatcca agaggtacga ccagattgat
    12421 tcaatatttt tcttttcgag gctctaatag gcatgcctat cagggctttt tttgcagact
    12481 gtgtttttat gcccacacga tttgtaagcg atttatgtta taatagatta tatctgcata
    12541 gaaaggaagg ttaaatggtt atcaataatt tgcaacagca agcttatgaa tctatacgta
    12601 aacaaatcat ttattccgag ttagcacctg gaagtaaaat ctccgataaa gtgttagaag
    12661 aacaactagc tatcaggcgt acaccgattc gagaagcact gatccaactg cgcaatcaag
    12721 agcttgtaaa aaccattcca cagtccggca cttatgtttc aaaaatcgat atccgatctg
    12781 cttcattagc ccgttatacg cgggaaaagt tggaaaaacc gatcctgcaa gaatgcagtg
    12841 cgaaaatgac caagcaagat caagctaaac tagaaaaaat actagaacaa accgatcagg
    12901 cagtaatcgc tcaaaataaa aaagcttttt ttgatttgga tactgatttt caccgaactt
    12961 gttatgaaat tgctggaaaa cgagaaattt gggattggct agaaagttat agtacgcact
    13021 tgaaccgttt cagatggtta cgtttgacga tttcagaatt agactggggt cgagtattag
    13081 atgaacatca gacaatgctt caatcaatga ttgatcataa ctttgatgaa gtcggatttc
    13141 tttgcacgat gcatttacat atgattatcg aggagcaaga atacgtgatt cataattacc
    13201 ccgattattt tgaagatatt caggacagac ccatcaaata agaggttttg gaatcagcgt
    13261 tttgacctga gtattagaga aagaaacggc aaaatagttc ctcatatttt gccgtttttt
    13321 ttgagctaat accgagggtc agctgattgc acaccgttta ttgagaggtt gtgaaaaagc
    13381 tgccctgcat caagtaataa gaacagccaa gtaaaaatag cttcttatgt ttttaacttg
    13441 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt tttccaagag gtcgtgagcc
    13501 tgccgttcat ctccgagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgttga
    13561 atattttgac ttgtgaccga ggagttgaca ggagaacacc gtttattcgg aaaaaaagga
    13621 ctgtgacacg acttttgtca cagtctcttt atttgtaatc taaatcatac tccgtacatc
    13681 ttttttctat tttttttatt tcttctaaga tcccttggct acgaatttca ttatatggcc
    13741 aaagtaagac tgcctctaat acataaggcg aaaaatcgct ttgattcatc tcttttgaaa
    13801 aactcatcgc tgctatctcg ctcaatgcac gaatgtttga ttgatgtgta taaaaaggca
    13861 accgccacag cacaattttt tcgctttgcg tataaattcc cttaacagaa gcttcgatgt
    13921 ggtgaaatag ttcatgtcca ataatcaaag cttgcgctgt ttctttagag atcgaagcta
    13981 gtcctgaaca ttttaatttt tctaacggct ccgtcatcaa tcgaatccct tcatcttctg
    14041 cataagttgc aaaagtgatt cgttcgttta tttgctctga ttcacaggaa acgattggaa
    14101 cagccatttg ttcacaaatt tctcctacat cggcaaaagg gtactttttt ttgatgctac
    14161 atgccgtttc gataccacaa tcgattgatt gttgtataag aattttctgc ttggacaaag
    14221 gaatcttttt ccaaaataaa tctcgccgaa actggtagaa tccccaaatt tcttcgggga
    14281 tctgttgtaa ttcgtttagt aactcttcta acttatgttt gtttgatatt tccggcaacg
    14341 acgatcactt cttcctccgg ttccatagta gatacttcca gatccatcaa aagctgtagt
    14401 tgttccagat ccgtcgctgt aaaatcagag actcttgctc ctaagcagag ataaaattct
    14461 ggtcgggcaa tcaattcagt ttgataaact gccatatctt cccaaagttg ttcgacagct
    14521 gtcatataat tggctgcagt tgttttcata catgaagcat gtccacgttg gaccttgata
    14581 aatccttttt gatcgatgat ccgaagactt ccagcatttt ttgaacgatt ctgttcgccg
    14641 catacataaa agaaaggtgt attttccaat aagcaaactt catttttatt taatcgcata
    14701 tcctctgcag caagctctag tgcttcttct gttgtgatct ccttggttag atcggttgct
    14761 ttcacttccg tagaaccagt agcgatagcc gttacttttg aagtctgtgg atcgatttct
    14821 acgtggactt cgatcgattc tggtgtagcg cctgattcaa tcgctttatt catcgcttca
    14881 tttttcaacg atcgtatatc ttctttactt ggcgaaggga tgatacgttc tacaacatct
    14941 cggaccattg caagtgctac accaatagaa gaaatcactt cggcgttttc aggaatcgaa
    15001 tatttcacac ccattttatt actaaagtaa gtaatcaatg aggcagcgcc gccccctaca
    15061 cctacaagag aaatctgatc tttctccaag tgatacttgt ctgccaattc taaaatcacc
    15121 ggttcaatct tagcatagga tttttccatg atctgttctg cgatgtcttc aaccgtcgta
    15181 tgacaataat cagctaatgc ttggatggct tttcgtgcag atgggacatt tccataagaa
    15241 aaatgttctt cttgcactaa tccaaggaca ttggctgcac atgtatttgt aatagtcact
    15301 tcttctccgt cttccatcac taccttgacg taatcagcag ggtctccttc tttaggagag
    15361 aaaaattcaa ccttaggacc tttgattttt tctgtctccg taaatactgc ataatctaga
    15421 ccggcaatat gtgcagaacg tggaccgaca tcgatgattc ctgactgatt agcccgtacc
    15481 atcgaaccgc ctgccacacc tagaacccga acatctaatg aagagatata tgttgggtgg
    15541 ccacctacaa ttgaataatc gattgctgga cgtccatttt taatgacccc gatattggtt
    15601 gttgttccgc ctacttcaaa ataaacacca ttagacgctc tcaagtacat caaagagccc
    15661 atgactgatg ctgctggacc ggaaagcatg gttaaaacag gtcgtttttt catttcattg
    15721 atttccatta ctccgccgtc tccgcgcata atcatcaaag aaacattgac ccctgcttct
    15781 cgtacggaat cttctgtaga ggttgccgta tctagcattt ttggtaaaat agatgcattg
    15841 attgctgctg tccgagttct tcttgtcaat ccatataatt ttgtgatgtc agaagccatc
    15901 gttgtaggga tcgattttac cgatgctgct tcataaacaa ctcgttctgg ttccccatta
    15961 tctactccaa atgccataga agaaaccaag acctccgctc tttctctttc taaagaagaa
    16021 atcgtttctg aaacacggtc tacatttaag tgtttgacag gtaagaatgc attgacaatc
    16081 tctatttttt tcttattccc taaatcgata tcgttcaatc tagtttgtcg ttttgctaaa
    16141 aaaccttcta atccaccttt tgccatcccg attacgccga cttttgctac gtctccttca
    16201 atcagtgcat tagtcgcttg tgtggtagaa tgtgcaacaa acacaacatc ttctggactg
    16261 atattatttt ccagcaaaca attttgaaaa gactgaacga cacctgcggc tactccacgg
    16321 acatcatcgt gggttgtttt cactgaagat tttccgatga tttcgtgcgt cgcattatcg
    16381 attgcgactg ctttcgtatg tgtaccgcca acatcaattc ccattcgcac aaaacgtttc
    16441 attttctttt cctccattca tctaatcgtt tttatcgacg ttttgtttac taggcgttcc
    16501 cgtacaataa gaacgtgatc aggcataaga tcgctccgat gatccaacca gttgggatgg
    16561 atttcttcat atattctcga gaagttactt ttgtataacc taatccccaa gcaacccaag
    16621 attgtgtaat atctaaatgc tgaggagcaa tcgttgtgat tgcaaataac gggaacaaaa
    16681 aggctggcga catcgttgga ttaacagcta atacgacagc taaaatagct gagcctgaac
    16741 cgactagatt cattggaccg cggaaaaatc ctagtggtgt caatatagca aatacgattg
    16801 ccaagactaa tggtgattgc ggtatccaat ctccaaaaat agctgaaaaa taaggagaag
    16861 catacgcagc tgaattattg aacatcgcta aggtcatcaa gaaaccaacc attggcgcaa
    16921 catctactgc gccatctgta aataatttcg caaactgacg acagatttct ttataacctc
    16981 cgcgcaattt tccagtaaac agcaatgccc agatacctgc tagaataaaa cctaagatga
    17041 ttggaagatc taaaagaacg acccctaaaa ctggtaataa tacggctaac catgagatca
    17101 ttggtgcgtc gccaccttca gctggtacgt ttgctgccat tgcatagcgt ttctttttgt
    17161 tcattgatat attagcgacc gttagtacca caactaagct tacaatcatc ccaatcatac
    17221 caatttggaa atagtcatta taagtatagc tttcagccgc tggattgaaa ccagcataaa
    17281 tcgtttgata ttgtttgaaa ttgacgatat tagcaaagat tccagccata atcgagccca
    17341 taaatgagaa cagtgcaacc ggtactgaaa tacctaatga catcaagatt ggcaacacaa
    17401 taactgcaat agaaatcact ggtccgattc ctgtcatcga catgaatata acagcggtta
    17461 caatacatag taagctcatc gtgattcttg gcttgtcccc tccaagttca actacctttc
    17521 gaatcagggt agctgcgatt ccgctatcta ctagaacacg cccgaaaaag gcaccaaaga
    17581 agacatttac caagatcgat ttggcgtact cagctggccc cgtcgtatag atatgcgtca
    17641 acacatcaat tactccttgc ccttccatcg ccgggtttgg tgaaaacgtg tttccgacta
    17701 gcgctaatcc catccataca gtagccatga tggcaaagcc gaccatcaag ttgtagcctt
    17761 ttacacagta ccagaccatg aaaaagaatg agataactaa caaaatacca ataataatat
    17821 cactcatgtt attcctccgt ccattttgtt tttctgataa gcaaactaat atatcagtca
    17881 aactcattct aacttatgta gttagcgctt tcattaaagg gtgaccataa atgttcagtt
    17941 ttcaattaaa aaaccttaat tatgctattt tagacttata cgttattgtc ctgtttcatt
    18001 tgatggagga ttgtaaataa aaaaagagga aagaacataa cttttcgtta tcttctttcc
    18061 tctttataat tgtttagctt atttagccaa agcttctttt gcttggtcag ccaatgcagt
    18121 aaatgctgct gcatcgttaa cagccaagtc agctaacatt ttacggttga tgtcgatttc
    18181 agcaactttc aaaccgtgca ttaattttga gtagctcaag ccattcatac gagctgctgc
    18241 gttgatacga gcaatccaca atttacggaa gtcgcgtttc ttttgacgac gatcgcgata
    18301 tgcgtagttg tatgaattca ttacttgttc ttttgctgtt ttaaataaag tgtgttttga
    18361 accgtagtaa ccttttgcta attttagcac tcttttacga cgtttacgag ttactgttcc
    18421 acctttaaca cgtgccatgt ataattcctc ctaataaaaa taaatcttca gattctgtta
    18481 tttgtacttc ttatttcatt tttgctagtt gttggcgaat acgtttgaag tcgccactag
    18541 aaaccatgct tgctttacgc aattgacggc gttgtttttt tgttttgccg tggaaacggt
    18601 gacttgtaaa cgcgcggaag cgtttcaatc cgcctttacc agtacgtttg aaacgttttg
    18661 ctgatccgcg gtgtgttttt tgttttggca tgactatatt tcctcctcaa aatctttcgt
    18721 tatccgacta tcggctgtcc aacagccagt tagtcattca aattacttac tgtcgttttt
    18781 cggtgccagc gtcaagaaca tgctgcgtcc gtccattttc gctttttgtt ccactgttgc
    18841 aatatccgca gtttcttcag ctaagcgatt caagactttt tgaccaattt ctttatgggt
    18901 aatggcacgg cctttgaaac ggatagaagc tttcacttta tcgccctttt ccaagaactt
    18961 acgtgcatta cgaagttttg tattgaagtc attcaagtcg atcgtaggac ttaagcgaac
    19021 ttcttttaca ttgatcactt tttgtttttt acgcgcttcg cggtctttct tttgttgttc
    19081 gaaacggtat ttaccgtagt ccatgattcg cgcaactggt ggtttcgcac caggggcaac
    19141 taacacaaga tccaagtttg cttgttctgc aatattcaat gcttctgctt ttgtcttaac
    19201 tcctaattgc tcaccatctg aaccgatcaa tcgtaactct cgtgcacgaa tgccgtcgtt
    19261 aaccatcata tcctttgcta tggtcattca cctccaaata ttatgagaga aagaaaagct
    19321 taccgaataa acggcaataa aaaaacgagt tctcttacag aacccgcgcg tacttcacct
    19381 aatagaacca gtgaaaacta tctagcccag tgacattatc aactaaggcg agaagcgggt
    19441 gcttctgctt ttatttctca accatcatag tatagcaaaa actaatcttt ttgtcaatgt
    19501 ttttcataca gtttgtcttc cttttctgaa aatgattaga ttagcagact ttctagcagt
    19561 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
    19621 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
    19681 gatcttctct ggtcttctga tcaacggggg tgaccaaagc tatctcgatg ccaaaaatat
    19741 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacagttt tacgtcattt
    19801 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
    19861 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
    19921 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
    19981 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
    20041 cacttggaaa aacctttttt tgtctgacaa tcagttgacc ggttcgaact ggtttcgaac
    20101 ttctctgaag ggattagact ttacgactaa tttcttcaca gcaatcgctt tatctcaaga
    20161 ttctttggcg ggactaatcg tcaaccaaga gcaagcactt gtgatcgccc aagcccttgg
    20221 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
    20281 gataagacca aactgatgat caaggcgatt ccccatccga aaacggaatg ggtaaagggt
    20341 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
    20401 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
    20461 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
    20521 tcgatcagcg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
    20581 aatgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
    20641 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
    20701 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
    20761 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg cattcgttcc
    20821 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
    20881 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
    20941 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
    21001 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
    21061 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
    21121 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
    21181 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggct
    21241 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
    21301 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
    21361 ttgtattctt cataccattg atcgtaacgt ctgttcaatt ctttgtaata atcgtataga
    21421 cttggatcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctccaacatc
    21481 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
    21541 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacgaacttc tgtttctgtt
    21601 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
    21661 aatacattat tgtcatcttt cattgcttgt tttatgcttg caaaacgttt attcaaaaaa
    21721 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
    21781 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
    21841 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
    21901 ctcacttcat cactaaatat tgtgtttaac tccaaccttt ttaaatatac ccccaatatc
    21961 tagtagtgtc aattgtattt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
    22021 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
    22081 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
    22141 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
    22201 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
    22261 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
    22321 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
    22381 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
    22441 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
    22501 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
    22561 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
    22621 cccacggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
    22681 ttacaaaagg gcatcctttc gactactttt ctttgaacat cgatgctttt ttgattgtcg
    22741 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
    22801 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
    22861 caaaacaagc aatcattgaa aattcaccag aaacgttggc acagacaatg gatgaatcac
    22921 aatcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
    22981 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
    23041 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
    23101 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
    23161 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
    23221 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
    23281 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt gcaagacgaa
    23341 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
    23401 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
    23461 gccagcgggc tagctgcttt agcaggcgct tgcaatactg ctcttaagct tggattagat
    23521 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
    23581 ggtggtttcg tcgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
    23641 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
    23701 aaagaagtgt ccagcagaaa tgggatgcgt cggacagtcg aaacatccag tttttatcaa
    23761 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
    23821 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
    23881 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
    23941 gtgcgacaat tgagaaagca aggcattcca tgctacttta cgatggatgc tggccctaat
    24001 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
    24061 tttagcaaag aacaagtaat tactgcacat gctggtccag gaattgcgat tattgaataa
    24121 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
    24181 gcagttgttg aaacaggcca tccagcagtt atcgctgcag tcgatcaatt cgtgacagtc
    24241 actgtagaat ctgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
    24301 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
    24361 tatatccttg ctgctattcg cttgactgaa aagtatgcac aagaaaaaaa cattctttta
    24421 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
    24481 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
    24541 ttgaatttat ctcagttgga gattttcaag attgcagcac tagccaattt agcagttcaa
    24601 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
    24661 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
    24721 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
    24781 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
    24841 tcgagagaag ataaaatggt ggcttatcag cttttcttaa aaaatagtac agaatgtgtc
    24901 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
    24961 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
    25021 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
    25081 gcaggtgggg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat tcttccttta
    25141 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
    25201 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
    25261 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
    25321 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
    25381 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
    25441 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
    25501 tcccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
    25561 ttgccaacat cggtgcaggg acttctgtcg aaagagccaa agaagctatc cgattgtttc
    25621 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
    25681 accgtgattt taccaattgg aaagtactta ttcaagaaac tcaaacagcc attgacgtcc
    25741 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
    25801 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
    25861 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
    25921 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
    25981 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gccgtgggta
    26041 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
    26101 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
    26161 cggccctaca ccaacaatct ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
    26221 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
    26281 gaacactttt gtctcacgct ctaaacacgt acaaacggcg gaagtaaaag caactccttc
    26341 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
    26401 cttggagtta aatgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
    26461 gtccttcggt attcgttcaa tccgctgatt tcacaacctc tccactaaac agtgtactaa
    26521 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
    26581 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
    26641 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
    26701 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
    26761 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
    26821 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
    26881 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tccggaataa
    26941 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
    27001 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
    27061 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
    27121 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
    27181 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
    27241 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
    27301 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
    27361 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
    27421 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
    27481 gcttgttcat tgtctacaac taaatgtttt gtttcatctg gtttgcccat catgtggaac
    27541 acacgtgtat cttcattggc ataatcaaag tagacataag cttccatacg acgttcatgt
    27601 gtatgacaag gcatcgtatt ccaagcgctt cctggttcta aaatcgtata acccatttgt
    27661 aattgacagc tttcgcatac atttggatgg atgtattggt agattttacg ttcattcagt
    27721 gtcaggcctt caccagtctc cattggtttt acttggtcga tactgatttt tacatttgga
    27781 tatttatgat gagctggaac agaactaata tagaatttag ctgggttttc agcatcttga
    27841 gaagaaaata caacatgtcg tgtttctttt ccgatatagt atccgtcttg ttttttcatt
    27901 tcttcttttt gcccatcgat ctcgatgaat cctggtccac cgatattgat cacacctagt
    27961 tcacgacgct ctaagaagta atcaacacct agttctttgt ctaatgtgat ttctaattct
    28021 ttttcagtag gtgtcactcc accaaagatc attcgatcgt tgtgcgtata agtcaaactg
    28081 attttccctg gttcaaatac cttttctact aaaaattctt ttctcaattg ttccgttgaa
    28141 taatgacgga tatcttctgg actgtgtgta taacgtgttt ccatttcttg cattttcttc
    28201 tactccttct atcgaatgat ttttccagag ccgcatgcta aaaattgtgc gacttcttct
    28261 tgactaaatt ggttgcagtc cccatggatc gtatgcttca aggcagaagc tgccgtagca
    28321 aaatcaatga tttcttgcgg agacatattg gataaaatcc catggagcac gccacctgca
    28381 aaagcatctc cgccacctac tcgatccacg attggggtga ttctatggac ttgggattcc
    28441 acatattcat ggttcatcca caatgtcccc ttcaattcat tttcacttgc tgaatgaacg
    28501 gtacgcttgg tcgaataaaa cacctgaata ttcgggaact tttcctgcat tttttgataa
    28561 taataaatta gaggttgttc ttctgcttct ggcgctttct cgatacctaa caaataaaga
    28621 gcatccattt ttccagctga gcagatatct actaaaggta aaagctgatg gattacttcg
    28681 ctggcttctt gctgtgtcca taattttcca cgatagttga tatcgaagct gatcaggcag
    28741 ccagcttttt ttgcagcttc tatcacctgt ttcgtcattt tttgccattt tttagacaaa
    28801 gctggggtga ttcctgaaac atgaaatata tctattcctt ggaataactc ctctagcgac
    28861 cattccaaat gatccatttc agcaaaactt gatccggcac gatcatagat cactgaggcg
    28921 gcgcgttcgc cactaccagt ttccatataa taagtaccta gtcttgatcc accagataat
    28981 acctgtttcg tatctactcc ataacattgc aagtgctttt tgactgcttt tcccaatgcg
    29041 ttatcaggta cttttgtagc aaaaatcgct tcatgtccgt aattagctaa agaaattgct
    29101 acgtttgctt cccctccgcc ataatgggca gcgaatcgtt cacttgaatc gatacgggtt
    29161 ccctgctctg tggacaatcg caacatgatt tctccgagtg tcactacttt tcccataagc
    29221 agctacctct tgatttcttt catttttgct gcatactgtt gcgcgacttc cgttactttg
    29281 tcaaaatctc ctgtagctgc gggtgctaaa aggttcccac ctactccgac tgttacgaca
    29341 ccagcatcga accactcttt catattgtcc agactcacac cgccagttgg catgatattg
    29401 acttgaggca tcggcgcctt gaaggcagaa atgatacttg gtccataagc gcttccagga
    29461 aatagtttga cgatatctac cccgcttttc agcgctgttt tgatctcggt aatagtcata
    29521 catcctggta agtaaggaat ttgatacaga ttgcacatct ctgccgtttc ttgatcaaag
    29581 gaagggctaa caatatactc tgcgccagct aagatagcca agcgagctgt gacagcgtct
    29641 aatactgtgc ctgcgccaat cactatttct ggttggtctt tgtagaatga caaaagttct
    29701 ttgattactt gatctgcttg agggactgta aaagtcaact caataccagt tagaccacct
    29761 ttgatgattg cgtggcaagc attcaaagct tcttctttgc tttttccgcg taccaccgca
    29821 atcactccgg cgtttttcaa tcgagataaa atatcaattt ttttcatctt ctccacctat
    29881 ttcctattta gaaattttat tttataactt ggaaatctat acccatatta tacggcagaa
    29941 aagaaaaatt gcaaacgctt ttttcattat ttttgaattc tttgctcgat ttcgcttttt
    30001 gtttgcttga gggcactgat tatttgttct tttgtctcat ctgttaaacg atatttcggc
    30061 atacttacac taaatgcacc aatgatttgt tcgtttttca cgattgaggc tcctatacaa
    30121 aaaatatcac gttcgatttc ttcatcgtca aatgcgatgt gggtttcttt taccgttttg
    30181 atttcctttt tcaaccgtaa tgcattggtg atcgtatatt ctgtgtaagg gatcaacgga
    30241 tgcttttcaa gataatcagt gtactgctct tcactgaatt ctgctaatac ggctttgccc
    30301 atcgcagaat tgtataatgg tcgggtgatt ccgatttttg aagacatgcg gatcgtttga
    30361 tgttttggat ctagtttgtt cacgtacaag atctcattgt tgtccaatac ccctaagtgg
    30421 atagtctcat cgatcttttg ctgcagctgt tctaaaaacg gtaaagtcaa ctctaccaaa
    30481 tcgatttgtt caatgttttt attcgcgtag cgaataagct ttgcacctaa acgatagttt
    30541 ttctctgaat ttttatttac gtagccgatc atcaacaaag tatctagtat tttcaagact
    30601 gtagaagagg tcattcccac accctttgcg atcgtctgca aagagacatc ttggttttcc
    30661 gctaaatagt ctagtatttt agaggctttg atcaatacag ttccataagg ctttttattt
    30721 tccatataat attctcctat ttctttataa gaaaaattat agcggaaata ccatcaattt
    30781 aaaagagata tccagatata aactaccatt tctctttaca ggcaatttaa tgttgaagat
    30841 ccgcttcgtt tttacatcaa actttgatat aaagcaatga tttcttctac tgtcgtttct
    30901 cttggatttc ctggtgtgca tacatctcgc aaagcatctt ctgcaattgc tggaatatct
    30961 gcttctttta cacctaattc agaaatggtt tctgggattc caaccgcttt gctcaaccga
    31021 tcgatctcac cacaagccgc ttctcttaca tcttctaaag acatttcagc tgctccttta
    31081 atccctagaa caagagcgat ctcacggtac ttttctccag tatattcttt gttatatttc
    31141 atgacagttg gcaaaagtgc tgcacacgct acgccatgag gaatgttgta ccatgcactt
    31201 aacgggtgag ccatcccgtg taccagtcct aaaccgacat ttgagaagcc cattcccgca
    31261 atatactgtc ctaatgccat tttttctcgc ccattttgat cgccatctac agaagcttcc
    31321 aaagaatggc cgatgatttc gattgctttt aaatgcagca tatcagtcat ttcccaagcg
    31381 ccttttgtga taaacccttc aatagcatgc gtcatagcat ccatacctgt tgatgcagcc
    31441 aattttttcg gcattcccat catcatatcg ctgtcgatga acgcgacgat tgggatatca
    31501 tgaggatcaa cacaaacaaa ttttcggtga tttacttcat ctgtgatgac gtagttgatc
    31561 gttacttctg ctgctgttcc agaagtcgtt ggtatagcta atataggtaa acatggattt
    31621 ttcgtatcag caacaccttc taaactaatg acgtctgaaa actctggatt cgtcaaaata
    31681 atcccaatgg cttttgccgt atcgattggc gaacctccac cgattgcgat caaacagtct
    31741 gcctctgcct ctttaacaaa agccactcca tcttttacat tttggatcgt tggattaggt
    31801 acgatcccgt cgtataaagc ataagcgatt gctgctttat ctagaagttc tgttactttt
    31861 gttgctatac cgtgctcgat caatcctttg tcagtaatga cagctgcttt gccgaatcct
    31921 cgctgctgaa attcagggac gatcgactca atcgctcctt ttccaaaata cgatgtttca
    31981 tttaaaatca ttcgattact cattttctat tcctcctaat ttgtattatt tttcacaata
    32041 aaaacgaaag aaccccaagc aaccggttgc ccagttgctt gttgctacag ttcaaagact
    32101 ggcatcaatt cccaactgac tggagaggca tctggatttg tctccatgat atcttccatg
    32161 tacttccacc atttttgatt gatagatgta tttgccattt tttcccataa cttttcatct
    32221 tcgatcacta agtaaccaaa taacaaactt gtactcggat caagatagat cgaatagtcg
    32281 atgaccccgt gttcacgaag tgctgtacgc atttctggcc aaagttcatc atgtcttttt
    32341 ttgtattctt cttgtttttc tgggtaaact ttcatgcaaa aagctttttt caccatgtca
    32401 gacacctgct ttcgtatgaa cttcctccaa ataacctgct tttggagtga caccaaacgc
    32461 atctgctaaa tgccacaggt tttcgtccgt gattgtttgc aagattggtc cttgcgcttt
    32521 gacgtacgta tagacttctg ctgccttttc tgctgtctcg atcaaaccaa agacttcatc
    32581 catgtctttt cctgacccgt agattccatg ttgtggccat aaaaccaatc gagtttcttt
    32641 cattttttca gctgttgctt ccccaatctc atttgttccc ggaaccatcc acggaatgat
    32701 ggaaattcct tctggaaaaa caactaagca ttccgtacac atttgccata gcgtacgact
    32761 aaaggaacgt tcgtctaatt catgcgtaaa actcatcgct aatagatgac tagcatggca
    32821 atgcataacg atccgattat ctgggtctac ttctaaacgg gcgatatgac tcatgaaatg
    32881 acttggcaac tcgcttgtag gaaggcattc gttttccagc ccccacaaaa gttccagtgt
    32941 tttgccatct tcagcaactc gaacgattcc aagattctcg gcaggttcgc tcgctacatt
    33001 tttaaaatat cttcctgaac cagttacaat aaaatagcat cctgctaaac ggctggcatc
    33061 aaaaatcatc gagatgcggc ggatcacttt ttcaggatcc aagaacggtg tgatctcttc
    33121 ttcttttagt aaataagaaa tattcccgcc atttctttca tcccatccca agcgataaag
    33181 attcgacgtc gtttcgatca tttctttcac ataaggcgct tgtaaaatat ctattttttt
    33241 caatatagcc acactcctat tttcttaatt ccaacacgtc tttttgatat tcctggactt
    33301 cttccagcca atccaaacca actggaactt gatttttgta acagaaataa ttccatacat
    33361 cggcatacgg gaaatctttc aattcttccg tataagccat tcttgaagta aagtcaaaag
    33421 ccagctccat ttcttccaat cgctcaactg gttccagcat agctttcagc agtgcttttt
    33481 gtgtatttct cgtaccgatg acccaagcag cgatacggtt gatcgttgca tcaaagaaat
    33541 ccagaccgat atttgttttt tccaacaatt cgttacggac taattcttta gcgatttcct
    33601 gcaattcatc atccatcaaa accacatggt ctgaatccca acgcactgga cgggaaacat
    33661 gcaacatgat tcctttgctg aataaagcta atgaagataa tttattggaa atcacttctg
    33721 ttggatgaaa atgtccagca tctagacaaa caagtttgtc acgagtcaac ccgtaaccca
    33781 tataaaattc atgggaaccg actgtataag cttcagcgcc taaaccaaat agtttgcttt
    33841 ctactgcatc ttgtacatac cgttcatcta actcttctgt aaaaatttca tcgagtgatt
    33901 ccattaaacg ttcccgtggg gtaaaacgat caatcggatt atctttcatc ccatcaggta
    33961 cccaaaagtt attgatactt gtttgcccag tttctttccc aaagtactcc gcgattttgc
    34021 ggcttctttt cccgtgttcg atccagtatt cacgaacttc tttatctgga tgagccaatg
    34081 taaagccgtc tttcatcatt ggatgcgaaa agaaagtcgg attgaagtca agaccgattt
    34141 cttctttttt tgcccactct acccattttt caaaatgttt cggctcaatt tcatttagat
    34201 cgacagattc ttctgtatcc aaatagatag catgaaggtt caatttgtgt ttgcccggta
    34261 tcaacgaata agccttctcc agatcttgtc taagctcttc aggtgttcgt gcggctcctg
    34321 gaaaattccc tgtcgccatg attccaccgg tcaattcgcc atccggtgtc aagaaacctt
    34381 tgacatcatc tccttgccag caatgcatag aaattttgat gtcctgtatc ttttctagta
    34441 cagcatctgt atctacaccg atagttgcat atttttcttt tgcttgttcg taatttcttt
    34501 caatcgtgtt catacgatta ctcctttgat tttattcacg taccattcca tcgtgttttg
    34561 cttattttca tctgggtaaa aagtttcaca tggaaacgac tgtttgatta catttcttgc
    34621 ttgttctata ttcgaaaact gacctgtact gagcatttgc atggcaacat tcccgattgc
    34681 tgtggcttca ctaggtcctg cttctacaat gattccggca taatctgctg tcaattgatt
    34741 cagtagacga ttattcgatc cgccccctac gatgtgcagc ttttccaatt tcgttccagt
    34801 taagtgttct actttgatca gctcttgtgc gtaacaaaga gcaagattat catacacaca
    34861 acgagcgata gctcccgatg agacagggac tggctgctct gtttcaaaac agtattgctg
    34921 aatctcttct atcatatttg ggggattcaa aaatcgaggg tcattgattt ccatcacttg
    34981 ctgcagcggt ttttcttttg cagcaagctc tgccaattcc gcataactgt actcgtactc
    35041 ttgcattcgg gcaacttcct ggatcaacca catccccatg atgtttttca aaaaacgcac
    35101 cgtgttatgg gcaccccatt cattcgtata attttcagaa aaagcattgc gagaaacatt
    35161 ggcgatcttt gtttcaatcc ctaaaagcga ccatgtccca ctgctgatat aaccccaacg
    35221 ttctccttga cctggcgttc ctaatacagc tgacgccgta tcatgacttg ctactgtgat
    35281 aaaggtcaca tctggcaaat catattttga aaattgttct ttttgcagat ctcctagtat
    35341 cgttcccggc tctgtcaatg gtggaaataa ttccttcgag acaccgattt tttcaagaag
    35401 ttctatttcc cattcttttg tccctgcatt cagcagctgc atagtagatg cattagtttt
    35461 ctccgtcacc atgttaccag taaagacata ccctaaataa tcaggtatca gtaacaattt
    35521 atgtgctttt gccaaatttt ctttttcttc agcaaacaat tggaacaagg tattgaaagg
    35581 ctggatctga atccctgtct gctcatacaa cttatctaga gaatagattt cagaaaactg
    35641 ttctactgct ccattggtac gcttgtcccg ataagcaatc ggatcattta tccgatcccc
    35701 ttgttcatca atcagacagt aatcgactgc ccatgtatcg atccccgcaa aaaggcgatc
    35761 aatccctgtc tgctttaatt tttcaaatcc aatcaaaatc tctttgataa gataatcgat
    35821 cttccagcga tcgtgaccgt ctttgttccg aaagccgttt ttgaaccggt gtacttcttc
    35881 caagatcaaa gaaccttttg catccctcgt acttttcatt agtcgtccgc ttgatgcacc
    35941 gatatcgatg gcaacaaatg ttggattcat ttctccacct cttttacttt attgttgatt
    36001 agcactttat taataaagcg ttatcattta ttgacaatgc tatcataata cagaatctta
    36061 tattttgctt cacactattt tggtatataa ttgcattatc ttgacaagtt gggagtgttt
    36121 acatggatta ctgggaaaaa gccaaccact catggtcgga agactcaaaa agagtgatct
    36181 tgacgcctac acaaagaagc aggaatttat ttttttatat ccaagaaatc ggttacttca
    36241 aagcaccaat cccttactat actgaacgag cacatttacc ttcttacttg ataaagttta
    36301 ctttgtctgg caaaggttcg cttacatata atgataatca gcaacagcta aaagctggag
    36361 acttattttt tattgactgc caagaatatc agcgttatca aacgatcagc gaagaaccat
    36421 gggaaatgga ttggatccat ttgaatggtc agaacgtcca atcattttat caggagttca
    36481 caaaagacgg ccataacacc ttccattcaa caggagatcc atcagaaaac aagatccacc
    36541 atttgatcac agaattgatc cacatgcaac aaggcacgaa tgcccgtacc gatttccagt
    36601 cctctgtagc tatccacgag ttgctgaatg aactgatttt gcaaaagtat cagctagatt
    36661 tcgaagaaga ggatattccc gcctacgttt acaaactgaa gcaatactta gacgaacatt
    36721 tcaaagaaac gattacactt gatcaattag aacaacaatt cttattgaat aaatatcagc
    36781 tgagcaaaga tttttccaaa tttatcggtt ctccaccaat cgattatttg atcagcaaaa
    36841 aactttctta cgctaaagac cttcttcggt atacagaatt tacggttcga atgatttccg
    36901 aagaaatagg gattgagaat tttgcttact ttagccgcct gttcaaagca aaaacaggac
    36961 tatcgccgag agactttcgt aaattcagct gattcatata atatgtacgt cattttttca
    37021 aaatattttg aaatatatag ataaaaataa aataaccgca gtttctgctt ctatgtaaac
    37081 aacagcagct actgcggtta ttttttagtg atcattctca ttgttcatct tcaaaatgtt
    37141 cataatgaac caactgtttc tgtgtatcac gtttcaaaaa ttctttaggc gttatgccga
    37201 actctctctt gaatgctttc agaaaatgcg gataagaaga aaaaccactt ttactatata
    37261 cagcactaga gctgcggtat tctcgcaaca agtgcttggc atagatcagc tttttcttca
    37321 ttacatactg atgaaacgta aatccagtgt actttttaaa ttctcgtgtg atatggtatt
    37381 tgctgacaaa aaactgcttt tccatttcct ctaaagaaag cggatgagat aaattttttg
    37441 agatatattg taacatttgt tccacacgct cattaggaac tacagcattc tgttcttgcg
    37501 cactctcttc tttttgtaca acttgattga gaaaaatcaa gtattctacc agagcttgtt
    37561 catattttat gtcttcaccg taaatttttt tatcaggagg atggtcgata aatgataatt
    37621 tttgcttcaa tacttcctgt tctatttgca atacacgact cctcttctct cccattggtt
    37681 gaaaacaagc cgatagatta gaccatggcg tggaacgact ttctagaaaa gaaggggtga
    37741 caaagatata gacacgctcg aatacatctg aagattggcg gacgatacga tgcaaatcat
    37801 tggaatggat gagcagcaca cttcctgctt tgatcgtaaa ttgtcgtccg ttcaaataaa
    37861 aagtcgcttc tccctttaat gtagcatgga tctcatagaa atcatgatga tgatagatcg
    37921 ttttattttg gctgacgaca tctttgacat gatagatttc atactgatca gaaaccatcg
    37981 tattgacttg ttcaccgctg attttctttt ggagcatgtg ccgcacctcc ttttgttcac
    38041 ttatgcacaa ttttattata ccacgttttt tgtaatcaaa aagagctgct tttatatact
    38101 cttataaaaa agaagagacg tggcatcttc tagccccgcc ccctccattc taaatattag
    38161 ttaaccaacg tcttctttac attattcaag aagacttctt ccataacgaa tcccatcatc
    38221 ttttgtttct tcaagaacca catacaggta cggacgattc ttcatgatga tttctttgat
    38281 ttttgtgtac tcgatcacac cgtctcccaa atttaccggc acgatttttt cctgttcgac
    38341 acgaaaatct ttcaaatgaa ccgcacatat cttttcacca aaacgttcaa aagcttcttc
    38401 taccaattgg acttgttctt gataatcact acttgtgatc aaattggtcg gatctaaaat
    38461 gattcctaga tagtccgaat ctactgcttg gatcagctgc tctacccttg ctaaagaata
    38521 caccggatga ttcaaacctg gttctatccc caccatcatt tggtattttt ctccagcttt
    38581 taccaagcga cggataacgg acacagcttc agcaaatgct tcatctgtga aattttcttc
    38641 cgtatattgg atttccggca aaacacatcc agtttccgaa gcaaccatcg acgcaccaaa
    38701 ggaagaagca tagcgaagat actgctcgaa ctttgtcagc aactcttgtc gtatcgttaa
    38761 atctggatga atcatattga tatagcaact taaaattcct acggtcactt cttgcttttc
    38821 taaaactcgt ttgacataat ttcccatccc agaattgatc tcatttgcgc cagaaggtat
    38881 ttcaggaaac gaaagaccta aggcgagctg cagtgtatga atcccttgtt cttttgtttt
    38941 ttctgcaaga tcttctatcg tttggacttc ggaaagatca tgtcctctaa ttgtaatgtt
    39001 caacataact attctcctaa ctcagcatat tgggtagcca catgacgatt tgcgggaagt
    39061 aagtaatcaa aaacaagacg actaagataa ccccataaaa aggaagaagc ggtttcagca
    39121 ttgcctctgc ttttacgcct cctacacttg caccgacata tagtcctgta cctactggcg
    39181 gcgtgatgga gcctacacac agattcatga ttgaaaacaa cccgtaatgt acaggatcaa
    39241 cccctacact tgtgacgatt ggtaaaaata ttggtgtaaa aatcaagata gcaggcgcca
    39301 catccataaa catacccacc agtaataaca caagattgac taataacaga ataataaatt
    39361 tattatccgt tacgcccata atcaacgaac taattgcctg aggaataccg gtaaacgcca
    39421 tggcgaaaga catcatggaa gaagtagcca ataagaacat gatcgtccct gacattgcta
    39481 ctgattgaaa caacatatta ggtatatctt ttagttttac cgttttatag taaaacatag
    39541 caatcaacag ggagtaaatg acagctatcg ctgaagcttc tactgctgta aatatccccg
    39601 tcaagatacc accgataatg atgacaatca atagaaggct aggaatcgcc tcttttatta
    39661 tttttccagc atgagccatc tgtccttttt cgacaacagg gtatttattc tttttagcaa
    39721 cgatgaatgc tactagcatg attcctagcg cccacaaagc gcctacgaga taacctccca
    39781 taaacaaagc agagatcgaa gcaccaccag caacaagtga atacatgata aaagcagtac
    39841 ttggaggaat gaccatacct gtcggagctg acgcgatatt gaccgctgtc gcaaacttcc
    39901 ggtcatatcc ttcttccact tgctgaggga tcaacactcc gccaatcgcg gtagaagcgg
    39961 caatggccga gctagagatg gaaccaaata aagcattccc taacacattc gtatgagcca
    40021 aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc cactagtttc ttagctatcc
    40081 ctccattatt catgataatg cccgagagaa cgaaaaacgg aatagcgatc agcgaaaaac
    40141 tattgatact tcccaccatt ttttgagctg aactaaaagc agaaacatca aacggtataa
    40201 ctaacaataa agtcatcatc gatgagaatg caatacttat agcaatcggc atccctaata
    40261 caagcaacac aacaaataca aaaaataaca ctaatccggc ttctattgcc attttattcc
    40321 tccattttta tattttttct tgaattgctg gcttgttgct taatgtatcg attagattga
    40381 tcaggctata gataatgatg agtacccccg atacaggtac tgacagataa acatatccca
    40441 taggaatact taaagaaggc gagatctgcc ccattgtcaa tgtgacagca ttccatccgc
    40501 catacaccat gatcatcgca gcaaatacca gaaataaact ttgaacaaca acttctaaaa
    40561 ttttactttt tttgccagtt aatttttcac ttaaaaccgt tacggctaaa tgtgcttttt
    40621 gtcccacaac atacgcggaa gacaacatgg caaaccagac aagaccgaat cgaacaagct
    40681 cttccgtgac cgtatttgga tttcctaaaa ccgttctgga aaatacttgc cataaaacaa
    40741 cagcgaccat cccaacaagc aaaacagctc ccaggatctc taataccttg tttaaaatca
    40801 ttttcatcac agctacgctc cttgctcttg gatcttttga taaaggtctt tgtaggtagg
    40861 atcgtttttg aatatatcat gaagtggggc aactgcctct cgaaacggtg tattatctac
    40921 atcattgaat tggactccat gttcttcttg tgctaaagct ttttcttctt caattgcttt
    40981 tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct gcttcttcaa tggctttttc
    41041 ctgctcatca gttagctgtt ctttgatggc atcattcatg atgatgatat ctggcacacg
    41101 tgtatgctca tcataggaat agtatttagc cacaccacca tgtccagctg tatacaagac
    41161 aaattcattg ttttctgatc cgttgatcaa attggattga agagacgtgt acacttcatc
    41221 acttcccata ggtacgggcg aaccgcctaa aagctctacc attttgatag cggtttcgct
    41281 ttgcatcaca cggatcttct ttcccttcaa atcttccggc gtatggatcg gaccgtctac
    41341 catataaaag ctgcgctgac cagaatcata ataggtcaat ccggtaaatc ctaaattttt
    41401 ggtcgattgg tagattggct ccattatttc ttcattatcc atcaccttaa agaaatgttc
    41461 ttcatcattg aatagataag gaactgaaaa aatcgaataa tctttagaga aactttccaa
    41521 cgctgaggca cttacttttg tgaaatctac tgctcctgac tgtgtcaatt cgatcagttc
    41581 acgttctcca cccagttgtc catctgggaa atactggatc tgtacggaac catctgtttt
    41641 ttcttttact aaacgcccaa attcattcat tgcatcaatt actggctggc tgttgcttgc
    41701 ataagcaaag cgcaaggtaa ctatctcatt tacatctgca tctgcgacag ttgccacact
    41761 agaattattt ccgcagccgc ttagacctag cagacctagg ctaagcgaac ttgctgcaaa
    41821 cagcagtgat gtttttttca tatgggtttc ctttctttta acaataagag acgagcaatt
    41881 ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat tgctgtgatt ccgatattcc
    41941 gataaaaatc taaactgtcc cccattcctg tcccgattga aacgttttga acttctcctt
    42001 tttcatcgat ttgttcaagc aatccttgga tagcacgata agcgacttct ttatattctt
    42061 gtggtaaata acgtttatgg accgctttca aaataccgta agcaaatcct gcagttgcag
    42121 aagattctac gtaagaagaa ggatcatcca acaacgtatg ccataaaccg ctttcagact
    42181 gataagtttt taatgcacgc acttgtgctt caagtgtttc gatcaataat ttgcgcaatg
    42241 catcttcttt cgataattct aagatttcaa tgatctctgg aatagcaatc gtcaaccagc
    42301 agtttccacg agcccaaaat gcttcggcat agttatgatt tccttcaaac gtccagccgt
    42361 gataccacaa tcctgttcgt ttatccatca agtatttgat atggatcata aactggtaac
    42421 gagcttcttc taaatattct gggcgattca acagtttccc gatttttgcc aaaggaagca
    42481 ctgtcatcat caatgtatcg tcccacaact gattcttatt ttccggtcca tatgtggcat
    42541 gctgtaaccc gtcttcattt gtccgaggca tgtcattcat gacccattct gcccattgtt
    42601 ctaaataagg caaatagcgt gaatctttcg tgtcttcata caagtaagcc atagtcaata
    42661 gcggtgccat tgtattgacg tttttgggcg gagcgccttc ttccatacga tcctcaaacc
    42721 attcattgac cacttgataa gcgcgaggat tttttgtcat ctggtaattt ttataaatac
    42781 catatagacc gacaccttgt ggccaattcc acactgtcca gcttttgtca tctactttca
    42841 gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt gatcgttgtc aggttttcaa
    42901 cgagcaggtc aattttttct ttcactaatt ctttaggtac aaaaatctct tctttaataa
    42961 tcatctttac gccacctttt taaacgcttt cattttatat ccttaatata gcagtaatac
    43021 cgtccactat aaatatcgct ttttgaacaa aaactgtcta aaattgacct aaatgttaaa
    43081 taacaaaagt aactttcagt tacttggaaa ttaaaaaaag aacttggaga aacaacgttt
    43141 tccaagttct ttttcatcta ttttcttatc ttttttctac gctttctttt gcttatccgt
    43201 tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa tggttctttc ggattagctg
    43261 tcgtacattg gtcttcgtaa gctaattcag ccatacggtc tacggttgta tctagtgttt
    43321 cttgtgtgac tccttgagct tttagactca tgttgatgcc gacagatttt cctaattcat
    43381 aaacagccgt tgctaaagct tcgactaatt cctctgttgt ttcgcctttc agacctaaga
    43441 attttgcgat atctgcgtaa tctgtatctg cacggaaata gtcatattta gggaacatcg
    43501 catgtttttg tggatctttt gcattatagc ggataatatg cggtaaaaga atcgcatttg
    43561 tccgtccatg agggatcccg tattccccgc cgattttatg tgcgactgag tgacaaatac
    43621 ctaggaatgc gttagcaaac gccataccag ccattgtaga agcgttgtgc attttttcac
    43681 gtgattccat atcaggtgtt tttactgatt tttctaaata gtcaaagact aacttgattg
    43741 cttgtaggct caatccacgt gtataatctg aagccatgac tgaaacatag gattcgattg
    43801 catgtgttaa cacatccatc ccggtatctg ctgtgacaga tgctggaact gacatgacga
    43861 attgaggatc gacgattgcg acatctggcg tcaatgcata atctgctaac ggatatttca
    43921 catgtgtttc actgtcagtg atgaccgcaa atggcgttac ttcagaacca gtccctgatg
    43981 ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc gatcttatat gtccgtttac
    44041 ggatatccaa gaatttttgt tttgcaccaa agaaagatgt gtctgggtgt tcatagaaca
    44101 tccacattcc cttagctgcg tccattgcag aaccgcctcc aagagcgatt actgtatctg
    44161 gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt atttgtcgat gggttcggtt
    44221 caacatctga gaagacttcg atcttcacat catttttacg tcgtgccaat acttcacgaa
    44281 ctgtatcgca gtaaccgaat tggaccatac cagggtcaca aacgatcatg acacgttcaa
    44341 cgttttccat tttctctaag tacaatagag agttcttttc aaagaagatt tttggcggta
    44401 atttgaacca ttgcatgtta tttctccgtt tcgctacagt tttgacattg attaagttta
    44461 cagcagagac gttacgagaa acagagttct taccataaga accgcatccc aaagtcaatg
    44521 acggaatcat ttcgttatag atatcaccga tgccgccttc tgcagatggt gtattcacta
    44581 aaatacgaca agctttcatt cgaagaccga atttgacatg caactcttcg ttttctgaat
    44641 ggataactgc tgtatgtcct aatcctccaa gattcaacat accttcacac aaatcaaacg
    44701 catggtccgt gttgttagct ttgatcatgg caagaactgg agaaagtttt tctcttgata
    44761 gcggataatc tgggcctacg ccttccaatt cagcaatcag cattttcgtt ccttttggta
    44821 cactgattcc tgccaatttt gcgatttcca tcgcagaatg tccaacgatt gatggattga
    44881 ccgcatattt tccttcgttc atcactgcat cttctaattt ttgcaattca tctggtttta
    44941 cgatataaac ttggtgtgct tggaattctg cttttactgc tgcatagatt tctttatcca
    45001 cgatcacagc ttgttcagaa gcacaaatca ttccattatc aaatgttttc gaaacgatca
    45061 aatcgttcac tgcacgttta atttttgctg ttttttcaat ataagctggt acgttacctg
    45121 gacctacacc taaagctggt tttcctgtag agtaagcaga cttgaccatt ccagctccac
    45181 ctgtcgctaa gacgatcgcg atacctggat ggttcattag catagaagtc gcttcgatcg
    45241 aaggatgttc gatccattgg atacaatttt caggagctcc ggcagcgata gcagcatcac
    45301 gtaccactcg tgctgcttct gcagaagatt tttgggcact tggatggaaa gcaaaaacga
    45361 ttggatttct tgttttcaac gcgatcattg atttgaaaat cgttgtggaa gtcggattcg
    45421 ttgttggagt tactccgcaa accacaccga ctggttcagc aatttcaatc aacccttttt
    45481 ggacatcttc attgatcaca ccaactgttt tatcatgttt gatattatgc caaatatatt
    45541 cggacgcata catatttttg attgctttat cttcgtaaac accgcggcct gtttcctcta
    45601 cagccatctt cgctaatggc atatgttgat ccaaagctgc cattgccatt tcatgcacga
    45661 tatggtctac tttttcttga tcaaagcttt ccatttcttt caatgctgca tttgcttttt
    45721 ctgccaaatc atcaatcatt gcttgtacgt caattgtctt cgttttttct ttttctatta
    45781 ctttagccat cattatcctc ctaatgggtc atggttattt gttaaataat tcacatgtat
    45841 atcatacaat atttattttt ctatgtaaac acttttttgt gatttttttc acagttattt
    45901 ttaaatataa aattgcttta tt
//
LOCUS       DADXTG010000006        45049 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.006, whole genome shotgun sequence.
ACCESSION   DADXTG010000006 DADXTG010000000
VERSION     DADXTG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.006"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..585
                     /locus_tag="IWI57_000259"
     CDS             34..585
                     /locus_tag="IWI57_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBC2448429.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(738..1211)
                     /locus_tag="IWI57_000260"
     CDS             complement(738..1211)
                     /locus_tag="IWI57_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2448430.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            1349..2821
                     /locus_tag="IWI57_000261"
     CDS             1349..2821
                     /locus_tag="IWI57_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBC2448431.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2818..3528
                     /locus_tag="IWI57_000262"
     CDS             2818..3528
                     /locus_tag="IWI57_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBC2448432.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            3880..4164
                     /locus_tag="IWI57_000263"
     CDS             3880..4164
                     /locus_tag="IWI57_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBC2448433.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(4445..4864)
                     /locus_tag="IWI57_000264"
     CDS             complement(4445..4864)
                     /locus_tag="IWI57_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBC2448434.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(4866..5450)
                     /locus_tag="IWI57_000265"
     CDS             complement(4866..5450)
                     /locus_tag="IWI57_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBC2448435.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(5497..6972)
                     /locus_tag="IWI57_000266"
     CDS             complement(5497..6972)
                     /locus_tag="IWI57_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBC2448436.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            7153..7464
                     /locus_tag="IWI57_000267"
     CDS             7153..7464
                     /locus_tag="IWI57_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448437.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(7579..9915)
                     /locus_tag="IWI57_000268"
     CDS             complement(7579..9915)
                     /locus_tag="IWI57_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2448438.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            10057..10725
                     /locus_tag="IWI57_000269"
     CDS             10057..10725
                     /locus_tag="IWI57_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2448439.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            10728..11771
                     /locus_tag="IWI57_000270"
     CDS             10728..11771
                     /locus_tag="IWI57_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2448440.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(12008..12889)
                     /locus_tag="IWI57_000271"
     CDS             complement(12008..12889)
                     /locus_tag="IWI57_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2448441.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            13048..13776
                     /locus_tag="IWI57_000272"
     CDS             13048..13776
                     /locus_tag="IWI57_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBC2448442.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            13932..14249
                     /locus_tag="IWI57_000273"
     CDS             13932..14249
                     /locus_tag="IWI57_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBC2448443.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            14242..14850
                     /locus_tag="IWI57_000274"
     CDS             14242..14850
                     /locus_tag="IWI57_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBC2448444.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            14905..15696
                     /locus_tag="IWI57_000275"
     CDS             14905..15696
                     /locus_tag="IWI57_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBC2448445.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            15834..16322
                     /locus_tag="IWI57_000276"
     CDS             15834..16322
                     /locus_tag="IWI57_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBC2448446.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            16373..17245
                     /locus_tag="IWI57_000277"
     CDS             16373..17245
                     /locus_tag="IWI57_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBC2448447.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            17421..17783
                     /locus_tag="IWI57_000278"
     CDS             17421..17783
                     /locus_tag="IWI57_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBC2448448.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            17792..18544
                     /locus_tag="IWI57_000279"
     CDS             17792..18544
                     /locus_tag="IWI57_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448449.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            18558..20360
                     /locus_tag="IWI57_000280"
     CDS             18558..20360
                     /locus_tag="IWI57_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448450.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            20637..21677
                     /locus_tag="IWI57_000281"
     CDS             20637..21677
                     /locus_tag="IWI57_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBC2448451.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(21729..23873)
                     /locus_tag="IWI57_000282"
     CDS             complement(21729..23873)
                     /locus_tag="IWI57_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBC2448452.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24230..25603)
                     /locus_tag="IWI57_000283"
     CDS             complement(24230..25603)
                     /locus_tag="IWI57_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2448453.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(25603..25998)
                     /gene="rbsD"
                     /locus_tag="IWI57_000284"
     CDS             complement(25603..25998)
                     /gene="rbsD"
                     /locus_tag="IWI57_000284"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBC2448454.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(26026..26943)
                     /gene="rbsK"
                     /locus_tag="IWI57_000285"
     CDS             complement(26026..26943)
                     /gene="rbsK"
                     /locus_tag="IWI57_000285"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBC2448455.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(27051..28064)
                     /locus_tag="IWI57_000286"
     CDS             complement(27051..28064)
                     /locus_tag="IWI57_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBC2448456.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(28145..28744)
                     /locus_tag="IWI57_000287"
     CDS             complement(28145..28744)
                     /locus_tag="IWI57_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2448457.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            28930..29631
                     /locus_tag="IWI57_000288"
     CDS             28930..29631
                     /locus_tag="IWI57_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448458.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            29641..32352
                     /locus_tag="IWI57_000289"
     CDS             29641..32352
                     /locus_tag="IWI57_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448459.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            32803..34431
                     /gene="treC"
                     /locus_tag="IWI57_000290"
     CDS             32803..34431
                     /gene="treC"
                     /locus_tag="IWI57_000290"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBC2448460.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(34491..35672)
                     /locus_tag="IWI57_000291"
     CDS             complement(34491..35672)
                     /locus_tag="IWI57_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2448461.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            35924..37447
                     /locus_tag="IWI57_000292"
     CDS             35924..37447
                     /locus_tag="IWI57_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBC2448462.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(37516..38244)
                     /locus_tag="IWI57_000293"
     CDS             complement(37516..38244)
                     /locus_tag="IWI57_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2448463.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(38257..39537)
                     /locus_tag="IWI57_000294"
     CDS             complement(38257..39537)
                     /locus_tag="IWI57_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBC2448464.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(39678..40280)
                     /locus_tag="IWI57_000295"
     CDS             complement(39678..40280)
                     /locus_tag="IWI57_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBC2448465.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            40525..40731
                     /locus_tag="IWI57_000296"
     CDS             40525..40731
                     /locus_tag="IWI57_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448466.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            41043..41762
                     /locus_tag="IWI57_000297"
     CDS             41043..41762
                     /locus_tag="IWI57_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBC2448467.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(41843..42526)
                     /locus_tag="IWI57_000298"
     CDS             complement(41843..42526)
                     /locus_tag="IWI57_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBC2448468.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            42744..44930
                     /locus_tag="IWI57_000299"
     CDS             42744..44930
                     /locus_tag="IWI57_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBC2448469.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        14602 a         7972 c         9048 g        13427 t
ORIGIN      
        1 tggatttaaa gatttgtcaa ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat
       61 attttgttag ggttattaag taagaagaag atgacaggat atgaattgaa tcaatcgttc
      121 aaaaatgaaa taggagaatt ttggcaagca aaacatagtc agatttatcc tgaacttgca
      181 aaaatggaag agcaaggaat cattcagcat caagtagaga tcacaggtga aaaattggaa
      241 aagaaagttt atgaattaac agaagaaggg aaggaacaat tggcagagtg gattcataca
      301 cccaccaatg aactaccggt taatcgagac gaatttgttc tgaaattgta tttcgtcaaa
      361 gacgtgaatg atccggcatt agtagaaatt attcagcagc agcgtgacct tcatgaagaa
      421 aaacgacttc atctattaga gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat
      481 gaaaattacg gacactacct gattttagcg catgcgatca atcgggaaac agaatatgca
      541 aattggctaa cagatgtatt agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca
      601 gtgatggaga attcctttct atatgaaaga aacgcagatc accgttcgga tattcttatg
      661 ttcaaacagg ccttttttca agtatttatg tagcatctac ttattaaaaa gcttggtact
      721 taggagatta gtggcgttta gagttctttt aacaactgtt cgtagtcagc tttttcatcg
      781 acaggaagga gcttgtaagt taattcgtct gtctcgttgt catgaatgat gtacatctta
      841 gcattctctt ctttgaagtt accgatttgt aaaatcgtat agcgttcgtc gatcaattcg
      901 atatctttga ttgcttttga aaaaacttta cggacatggt gcattttctt ttggcggatt
      961 tctgcctttt ctgcagcttt tgacaaagta aatccgtcat ctaaatattt tttgatcagt
     1021 tctactttta tgactgtagg taaacgatat tttctaggac aattcgcatc agcttcgatc
     1081 gaacgaatga atcccttttg ttcccaatac cttaattgac gtggagacac acctacgata
     1141 tcacttaatt cactgatgcc aacaaccaat tgatcatttt ccaacaaacg tttaagggaa
     1201 gccccaacca tttttgtcac tccttttgtc ctcttgcttc ccttaattat atccgtatta
     1261 acttatgtgt caagtaggtt gacaaatagc gaatttagga ctaaagtatt ctagtagttt
     1321 cagaaaaaat tcagaaagaa ggagaaatat gagtaacaac caacccgtag atatttacgg
     1381 aaaaccatat aatcggacac ttttagttgt tgtcttatta attggtacat tttgtaccgt
     1441 tttgaatcag accttattga cgacagcttt tcctacattg atgaaagctt tcgatatttc
     1501 agcgtctgat gtgcaatggt taacgactgg tttcttgcta gtcaacggga tcatgatccc
     1561 aataacagca tggctgatca ataaattcag ttcaagaaat ttatatcttt ctgccatgac
     1621 gatctttttg atcggaacga ttacttgctt tacagcacct aattttagta cattactaat
     1681 cggtcgtttg atccaagctt gcggtgtggg agtatcgatg ccattattgc aaaatatcat
     1741 gctatctatt ttcccgccag aaaaaagagg atcagcaatg gggatgtcag ggatcgttat
     1801 tggacttgct cctgctttag gccctacatt atctggttat atcattgata actaccattg
     1861 gcgtgacttg tttggtatgg tactgccaat cgttgttctt gtcttggttt tagcattctt
     1921 cctaatgaaa agtgttattc cattgtcaaa tcctagcatt gatattttat ccgctatttt
     1981 atcaacgatt ggattcggta gtttacttta cggattttct agtgtaggag atgacggctg
     2041 gggaagcttg aaagtaattg gtttcttagt tgttggtgtg attttcatcg ccttgttctc
     2101 atggcgtcaa ttacatttgg aacatccatt cttggaactt cgtgtattca aatcatctac
     2161 attcacgatt gcagctgttt tagctggtgt aacaaatatg gcgatggtag gggcagaaat
     2221 ggtcgtacca ttgtatattc aaaatattcg tggagaatca gcattccatt ctggcttgat
     2281 gttgttgccg ggtgcattga tcatgggggc tatgatgcct gtgacgggtg cgattttcga
     2341 taaatacggc gcgaaacgtt tagccatcac agggatgttc attttgacag cggcaactgc
     2401 accatttgct ttcttgacaa aaacgactcc agttttatat atcgtgatcc tttatgcgat
     2461 ccgtatgttt gggatctcca tggtcatgat gccggtaacg acttctggta tgaatgcatt
     2521 gccaaataac ttgatcagtc atgggacagc ggtaaataat acattccgtc aaatcgcaag
     2581 ctcgattggt acagctattt tgatcagtgt gttgacgaat gtgacaaaag caaatatccc
     2641 aagcaaatct gtattggata atacgccatt agcttataaa gatcaggcaa tcaatgccac
     2701 actgtcaggt taccatgccg cgtttatcgt agcgatcatc tttgggattc ttggctttat
     2761 tattgccttt ttattgaaga aaaaagaacc tgttctaatg aaagcggggg ataatgcatg
     2821 attattggag ttttgattgt cagcttgctt gcttttgctt taacgaacgt gttcgctaaa
     2881 aaaacatggc aaaccttttt atctttgatt ttcggactaa tcttcgtcgc ttcactaagt
     2941 ttgatcgtcg ctaatttgtc caatcacttt ggaatggaaa aagtaacgga aacaaaaaca
     3001 gaaaaaattg tttccagtgc tgattcccaa ggagcagata tgcttttgta taaagcttta
     3061 gggaatggaa aagaaaaagt gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa
     3121 gcaactggta cggataacga aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa
     3181 gtgacgaaaa ctacttattg ggaatacaaa aacgacatgt ataaattctg gttcaatatt
     3241 gctgataaca atcacgaata tgacagccga gtgaatacat tcaaaatccc agagacatgg
     3301 gtagaattaa gtacggacca agcagccaag ttagctgaat tagtgaagaa acagcaaagt
     3361 acgatggaaa gtgaagcaaa agcttatgtt caagacggta tggtaaaagc aatgactgaa
     3421 aatccaaaaa tgagcaaagc agagcaagaa caacgtacga aagccttagc tgctgaattc
     3481 caacaacaag cattcgcaaa attagtgaaa gaagcaaaag gcgaataata ttttgaacac
     3541 catgtaggag gttgtgaaaa agctgctctg catcaagcag taagaacaat cagataaaaa
     3601 tagcactcca tatttttcat ctgattgggc ttagtgccgt agatgcaagc ttttgaacac
     3661 cgtttaagga agaggttgtg agcctgccgt ttaactctga gtcacaagga gcaatttcaa
     3721 aaaacagctt ctcatgtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa
     3781 caccgtttat tcgaaaaaag gaactgcgac aagacttttg tcacagttcc ttcttttttt
     3841 tgctatgcta tactagatgt aaaaaatagg agaaataaaa tgagggaaat ttattctttt
     3901 gaatcaaaaa tctatgcttc cgaagtcggc aagggcgggg catatattat ttttccgtat
     3961 gatataagaa aagaattcgg tgctggaaga gttaaagtcc aagcaacttt tgacaatgtg
     4021 ccgtatgaag gcagtatcgt gaatatgggc gtgaaaaatc cagatggttc agtctgctac
     4081 atccttggac tacgaaaaga tattcgtaaa aacatcggaa aagaaatcgg tgatattgta
     4141 gcagttacag tgaagcaaaa ataaaagagg ttgtgacaaa agacgtgtca caacctcttg
     4201 ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca aatttatgca aaaatgtgga
     4261 aacccatgtt tacaaaattt ttcttactac tcgaaacgaa gcggctttat cacaacctct
     4321 tgataaacgg cgttcaatca gctgtccctc ggtattagct caaaaacggc aaaatatgag
     4381 aagctatttt tccatttttt tctctcatac tcaggtcata acgctgattt cacaacctct
     4441 tctttcataa tggtttttca ccatctttat aataaactgg ttctttgata tggaaatcta
     4501 ttttttccaa ccaatctaaa atcttgtaac ctaactcttc tgacatcgga aaagaggcgt
     4561 tgatttgtgc attggagata tggtcaacat ggataccgga agtgatcgta caacttcccg
     4621 gaacggcagt tttgatgatc acagctattt tttcggctaa gacatcgtct ttatggaacc
     4681 gaccatcgtg gctagggaaa cgtacagttt gcatctctgt atcacgagaa aatgtagtga
     4741 ctgttccgat atgtggtgta tctccgcctg ttaaagtgat caataagtct ttaccgatta
     4801 ctttaacagc agcttggatc gtgaaaccta gtttggttac ttcaactatc gtttcttttt
     4861 ccatattaat caccctgcca acgttttgaa tgctcattta caatttcaaa ttgatccaaa
     4921 agtttcattg tatggtgatt aacaagatct tggatcgttt gaggctgatt ataaaaagca
     4981 ggaattggtg gtatgatttg cacacctagt cgtgctaatt tagtcaagtt ttccaagtga
     5041 atggcattta atggcgtttc acgaggaaca atgactaact ttcgttgctc cttcattgtt
     5101 acatcagcag cacgtgcaat caggttgtcg cctaaaccaa cagagatacc tgcgactgtt
     5161 ttcatacttg ccggtacgat gatcattcca tcggtcaaaa atgatccgct ggcgacagca
     5221 gcacctaaat ctttgtttga ataatggtaa tcaaacaggc tttcgaattc agttagtgat
     5281 aagtcggatt ccagctttaa gttctgtttt gcccatggac tgatgattcc gtgtgtttct
     5341 atcatcggat attcttttag tttcttcact aggttgatgg cataaatggt gcctgaagcg
     5401 ccacttacac caatcacgat ttttttcttc cgttgtgcca attgttccat tttccgcact
     5461 cctttttatg atgtcaaaac agttgcagat cattcattat ttcaaatatt tttcccaatc
     5521 ttctacttct tggaattttg cacgtacaaa atgttctttc atatcgaaag gaactgtacc
     5581 gtcaaagata gtttttgcac ttatcccacg gaaacgaata gagccaggat tatattctgg
     5641 taattctgat gggtccaatg gatggttcct cattcctggg atcacgatga tatctttatc
     5701 tccttggaaa cgagtgttga aagtccaaac tacatcgttc atgtcgaaaa tatcaacatc
     5761 ttcatcgact aaaatgactg tcttcaattc tttgaaggct gataaagcaa gaatagctgc
     5821 ttgacgctga ataccttcat ccgcttcgtt ttctttacgg atttgcatga tggtcatcaa
     5881 tttaccacca cctgcaggag gattgtagac atttactact tttcctggaa tagctttatc
     5941 gaccaaggca agaatacttg cttctgtagg aataccggcc atagatacgt gttcttcact
     6001 gggaccaatc gttgtttgca tgattggatt gtcttttcgg tgagtaactg ctttgacttt
     6061 cactacttgt acagctggat tagcatcccc agtatagcca ggaaattctg gcattgcttt
     6121 tcctgagttc gtgttgatat cttcttgcat tgtttcattt ggcatgattt ctgcttcaat
     6181 gatgaattca gcacgagcaa tacccacttc atcgactgct ataccatcaa cgatttgaat
     6241 aggttcgtta cgcaaagctc ctgcacacca tagttcattg tagccaagtg gagtggtagg
     6301 tggttcgaaa gtcgtgccaa tggcaattgc tggatctaaa ccaatattga tagtgatagg
     6361 cattggttta ttcaaggctt cgaattgttt ttggaagtga ccaatgtggc gtccgccagg
     6421 catgatgtac ataccgatcg tatctttatc ttctagaacc atacgatgga tcgttacatc
     6481 gctcattgtt ttttctggat ctgatcctaa gactacaccc attgtgatgt atggaccagc
     6541 atcttcagga gtatttgtag gtgcaggcaa aatgttgcga atatcgaagt ctgcatcggt
     6601 agctagatga acgacttctt gtgctggagc gtctgctttg tctactttca caggtttgat
     6661 tggattttct acagcgtctt tcaagaaacg accaagtgtt cgataatcat ggtgcaaaat
     6721 ttcacctaca cgtttacgac tagccattgt tccgatgttc acacgtgtgc cggggaaacc
     6781 tttgatattg ttgaaagtca tagctggtcc ttcttgagtc ggacgtttta ctgtaccgcc
     6841 agcaccgata taacggtaaa caccagataa ttctgcttca ggatcgattt caatatctgt
     6901 ttcgtgatat tgacctggaa gtgtttttag ttcttcgatg acttttctta aatcatacgg
     6961 ttgatctgtc atgagagatt cctccttagt tgtctttacc tatctatagt aaaaggtgaa
     7021 aaagaggaaa acaattcaac ttgttcggta aagtattcta caatggaatg gatgcgtttt
     7081 tttgataata gaataaacta tgatatactt atcactaaaa gaaagcgcat acttagtgtt
     7141 ggaggaagga aagtgaaaaa aatggaggag tcatttccta aagcagtaaa agtggaaaat
     7201 atagcgaata ttttgaaagt tacatttgaa aacggagaag taaaatacgt gaaatctcat
     7261 tggacagaag agataaccga tgccttgcaa tttggaaaaa aaggaagagg aaaaagaaaa
     7321 aatcttctcg cactttctcg taatatgtgg attgggactg aagtaacgat tgaagcagat
     7381 ggaacagtat tcataaatgg aaaagacagg tatacgccag aagagttgtg gtacaaagga
     7441 aagaaaagta taccagaatt ataaaaaata attaaaaaaa acagcgcgaa acaaggaaca
     7501 tattacttcc tcgtttcttg ctgttttcta ctatttcagc gctcttatga ctcaaatcta
     7561 aatgtggttg tcacaacctt attctgcacg caaagcgata gcggcgtctt ttttcgctgc
     7621 cattcgtgca ggaagatgac cgccaagcat cgtcaatata gtagagacga tcactagaat
     7681 aatcgcatgg acaggattta gttgagcaac attttttaga tccgtcatgt tgtataagat
     7741 gctattgata gggaacgtag ctagccatgc aataaacaca cctaatatcc cagaagaaat
     7801 acctaaaata cacgtttctg cgtcaaatac gcgagtgatg tcttttttac gtgcacctaa
     7861 agcttttagt acaccgattt cttttgtccg ttcaatcaca gatgtataag taatgatact
     7921 gatcatgatc atacttgtta ctaaagagat ccctgcaaaa gcaataagga cataagtgat
     7981 agcatccatc aatccgccag tcagttcagt catcgttcca gctaaatcag tataaatgat
     8041 tttatcttca tctgatttgc ctttattgta atcatctaga taatccaaga tcttttcttt
     8101 gtcttcaaaa ttgttcggat aaatcatgat acttgaaggc aagctgtctc cacctaaata
     8161 actaagcaat gtttgtttgg cattttcgtc aactttttca gtagttaaga catttacacc
     8221 actgtctctt tgagctttga cgatttctga ttctttgttt tcgttgacga tttgagtcgt
     8281 taattgatcg ctataagcga ttcctggaga taagagattc attgtagaag aagatttcac
     8341 gcgtaggatc cccgagatct tcaattcatc agaagcattc tgatacatcg catcataatc
     8401 tgtattaggg ataaaattcc cagttggaag ttttgtatag taagtattgt tgtttactaa
     8461 tttgaatgtt gtaccaacga tatcatcaaa atcaagtttt tcgtcttctt tcacatcaaa
     8521 tccaagattc ttcaatgcgt tgatattagt attattattg ccgtctacga tcagcacgac
     8581 atctgttgct gaggctggat aacttccggc aagtaaagaa taattgtctt ttaaaaagtt
     8641 ttctttgcta gtgtcaagct gtgtagggaa acttgacact cccacacctg tcattgctga
     8701 catcgtacta gataaagaaa gagattccgt atcaggattt tgattcgaga agctgacagg
     8761 ctgtactttg ccatttacat cacgtaaaag attgattcct gtagtacggg taaatccgat
     8821 attattactt aagttaggat cgatgtctgt tacgtaatcg acgtatgttt gatcaatctt
     8881 attcgtgtgc tgtgctttat cttcttcgct gacttttgca gtaatcgttt tgctgtctgg
     8941 ataatccgct ttgcttgcac ccaatccggc atcgtcacga gtctggttag tcgttacctt
     9001 agaaatagta atcggaaatt tcgccatcgt ttcagcttgc gtgttatcga tttgtttttg
     9061 gaatccagaa gaaagggaca gaacgatcgc gattccgatg atcccaatac tggaagcaaa
     9121 ggaggtcaag aaagtacgtc ctttttttgt tcggatattg ttgaaggaaa gctttaacgc
     9181 tgtccaaaaa ctcatctttg ttctgcgtaa gttaaattga tcatcttttg gtcgttcgat
     9241 gtgaggatgg gaatctgtta agatcttacc atcagaaaat tcaatgattc gatctgcgta
     9301 ttgatgggcc agttcaggat tatgtgtgac catgatcact aatttttctt tcgataactc
     9361 ttggatcaat tccatgatct ggatacttgt ttctgtatct aacgctccgg ttggttcatc
     9421 gcacaaaagg atatctgggt cattggctag tgctcgagcg atggccactc gctgcatttg
     9481 tccaccagat aactgatttg gctttttatg catatgattt gtcaagccga ctcggtgcaa
     9541 tgcatcttct gcccgttttc gtttttcgtc tttagaaaca ccacttaatg tcatcccaag
     9601 ttcaacgttt tcaataatgc ctagatgacc aatcaagttg taactttgaa aaatgaaacc
     9661 gatagagttg tttcggtagg catcccagtc actgtcctta aagtctttag tagactttcc
     9721 gttgattacc atatcaccgg aatcataatt atctagtcca ccaatcacat tcagcatagt
     9781 cgttttacca gaaccactag gacctaaaat cgctacgaat tctttttgtc tgaaagcgac
     9841 agacacacca tccaatgctt tggtggtggt ttcgccgact ttataatatt tttttatatc
     9901 ttttaattga agcataatca cccttctccc gtattgaaat attcggtata ctaataagta
     9961 tatcagaaca atgatcgtag tatacgaaat aaatgttaac tgaagatgaa ctacaagaaa
    10021 aaatctagaa atttttcaaa aaacagaggg agatcaatga atcgtatttt agtagtagaa
    10081 gatgatccgc aattacagtt gctttaccgt tctgtgttgg aacgagcggg cttttcagtc
    10141 atcaccgcat tgaatggaga ggatgcgctg aaaaaactag aagcgacacg tatagaatta
    10201 atcattacag atatcatgat gccgaaaatg gatggctatg acttgctaga ctcattacgt
    10261 agcaccagat ttgacacacc tgttctaatg attacggcaa aggccgattt tgaggataag
    10321 aaacgcgggt tcaaattagg agcagatgat tatatgacaa aacccatcga tgtcaatgag
    10381 atgatcctac gtgtggaagc attgcttcga cgttcaaaga tcaattacag cagtagcttg
    10441 ataatcggac aaacaaaatt agagctagat gcttatacag tgttacaaga tcagcattcg
    10501 ttgattctgc ctcaaaaaga atttctgctt ctgtataaat tactgtctta tcccaacaag
    10561 atcttcactc gccaacagtt gatggatgat atttggggat tagatacgaa tacagaagaa
    10621 cgaacgatcg atgtacatat caaacgttta aggacccgtt ttgaaaagac ggaagatttt
    10681 caaatcatca cagtacgggg actaggctat aaggcggtta tttaaagatg aaaaaaaagc
    10741 tattttcaca attatggatc tattttgcta gtattgtctt catttcgatc ttagtgacac
    10801 tgctgtgttt tctcggtttt attttcttat tgtctcgaca ttctatctcg cctgctgaat
    10861 cggctggaaa accaacgtta tacccgttat tcgtctttgc ggggttcagt atggtcgtgg
    10921 gtacaggaat atcgattttt gttgggcggc gtatcctgca tccaatcagt gcgctaggga
    10981 caaatatgag tctggtagca acgggagatt tttcgattcg aatggatgaa cagcagaaag
    11041 tagcagaagt acagcaactg tataaagatt tcaacgttat ggtccaagaa ctcaatagta
    11101 tcgagacact gaggaatgac tttgtgtcca gtgtatccca tgaattcaaa acaccactag
    11161 caacaatcca aggatatgtt caattgttac aagccccaaa tctctctgac gaagaacgcc
    11221 aaatattttt atatcggatt atcgaaagta tcacgcagct ttcacaatta acagagaaca
    11281 tcttaaaatt gaacaaactg gaaaaccagc gtatccaact ggaaaagaaa gagtatcgat
    11341 tagatgaaca aatcagagaa gtaatcgttt tttttcagcc taaatgggag aaagaacagc
    11401 tagaattgga tatcgaactt gctgcagtaa attacacagg caatgaagag tttttgtacc
    11461 aagtgtggct gaatatcatg gataacgcca taaagtacaa tcaaatcaat ggacagattc
    11521 atatcaaact atttgagaca gccacagaga ttgtactgga agtgaccgat tcaggtgtag
    11581 gaatgaatga agagaccaga gatcgaatgt ttgaaaaatt ttatcaagga gatacaagtc
    11641 gtcaaatttc tgggaatggg ctaggacttt cactggttaa aaaaattcta gaactgcatg
    11701 atggacgtat tgattatagc agtatcgaag gagtaggaac gacagcaatg atacggttaa
    11761 gtaaacaata ggaaaaagaa taaaacccct aaaaattcta ttgaaggttt ttaggggttt
    11821 ttagtcgttt ttttgtgatt agggaaatgt tcttacggtt cttttggcat aaaaatcgct
    11881 gctttcttat tgaactacga gaaatgttag aggtgtcatt gaaacaagga catgatgcaa
    11941 gaaagagagc gaaagtcatg cgtcgctctc tttttttggg tggatagatg gacagaaaat
    12001 gtagggctta gttttcttct tgatttaaat actcccaaaa tgtatctgaa gccaaagaat
    12061 gctcaccgac tttacgagta aatgcaagat aactctctgt tgttggagaa atagggagaa
    12121 tcacagtatt ttcttttaat agattcttga ctgttttttc cataacaata gcaatcccca
    12181 tgtcatttgc aatcatattg atgatcaagt cgattcttgc tcctttataa gagatttttg
    12241 gttcaaatcc agcttcgcga cacagatcta gtacaggttg atacaagttc gtagtggggc
    12301 caaggagcaa aaagttctcc tttttcagtt cgcttagatc caatttcttt ttactagcta
    12361 aaggatgatt tttaggtaga actgctacaa aatcgtctgt ttcagtaggc aaccactcaa
    12421 tcgaacttgg tggggcctgg aaacttctag caaagtggat cgtgtcacta tgttcattgg
    12481 cgtcattgta agttaattcg acactttcca cttctttcaa ctggactgta aattcaggat
    12541 ggcgtttcaa aaactcggtg attttggaaa aacctttata attgatcatt gtcggtatcg
    12601 ttaagatatt taatgaaaga ttgttgtctt tcgtataggt ttcaagcact tcttgcatag
    12661 catggtattg ttctacgaca ttttttacat atgggagcaa taagcttcca gcttcggtca
    12721 attcgatttt tctatgggcg cgttcgaata atttgacgtc taattctgtt tctaacgaaa
    12781 gaatctgttt agaaatattt ccttgggtgg tgaattgtcg atcagcagtt ttggtgaaat
    12841 tcattgtttc ggctaaatca agaaaaactt cgaatttatg gatatccatc atattcctcc
    12901 tacttattcc aataaagaat actttaacac aaaaaatgaa ttggtgtaat cttctctgtc
    12961 tgttacaatc tttcttgcag cgaaagcaat aattacagaa atttttctga tttttttcgc
    13021 ttcgtagaaa aagagaggag ttttttgatg ccaactggag ttttgatcaa tgtaggttcc
    13081 gttttgtttg gcggactaat tggcggatta gtaggaaata aattaagtga acattttaaa
    13141 gcacagctga cgatggtttt tggtgtgtgt tcgatgggga tgggaatcta ttccattgcg
    13201 ccgatgaaaa atatgccggc tgttattttt gctttagtga taggaacagc gattggtttg
    13261 attgttcatt taggtaatgg gataaataag ggggctgcgc tgatgcaagt acctatttct
    13321 aaaatcttcc ctagtgaaaa gcttggtatg acccacgatg aatttatcag cacattagtg
    13381 acggtcattg ttctattttg tgcaagtggt acggggatct atggaagttt agattctggg
    13441 atgacaggag acagtacgat tttgatttct aaatctgtcc ttgatttttt cacagcagct
    13501 atttttgcgt gtaatttagg gtatgtcgtt tctgtgatag caattcccca attcattatt
    13561 ttttatattt tgttcttatt ggcaaaattt atttatccat tgactacacc agacatgatc
    13621 ttagatttta aagcttgcgg cggattcttg atggtcgcta ccggttttag aatgatcaac
    13681 gtgaaaatgt tcccagtagc tgacatgata cctgcaatga tcgttatcat gccactgagc
    13741 tggatgtgga caaattggat tatgccactg ttataataaa aatcaaagag aagccatttt
    13801 ttgctgacta aaaaggatca aatcttttag tcagtttttt tgtatttgac aaactgattt
    13861 tgaaactata ttatatgttg tataatatag attgaaataa aatattacac ttgagaatag
    13921 aaggtgacgt aatgcacatc cagattccaa ctgttcttct tgatggtact gtgctagcag
    13981 cattaagcaa ggaagatatg tatgggtatg ccttaacaaa aatcgtacaa gaaagtctct
    14041 ctgtcagtga atctacacta tatccggttt tgagaagatt gaaaaaagaa ggatatcttg
    14101 agacctatga tgaacctttt gaaggcagaa accgtcgata ttactcgttg acaccaatgg
    14161 gaagtcgtca tttagaagaa attattgatg aatggattgt ttttcgagat tctttagacc
    14221 ggttattgga ggagagcaca aatgaatgaa tatttgaatg aagttgtgga tcatcttcaa
    14281 gaaattcctg aagacgatcg ttttgatttg atccagtatt atgaggaata ttttttagat
    14341 tctggaaaaa cattagatca gatcattgat gaatatggta caccaaagca gtttgcctta
    14401 aaattaaaaa tcagttactt ttcagaagaa gaacaagcag aaaacgagtt agtgaaaaat
    14461 ccaaaaagac aaattcgcct gatttggtta atcattatcg gtttatttgc ttctcctgtc
    14521 ctcattccac tagcactggg gtttatattg gttattgggg gactattggt tgctttacta
    14581 gcgctcattt ttagtatcta tgttttatgt atcagtatct taggtgttgg cctattcatg
    14641 atcatctcag gaatcagtgt gtttggacaa tcgattctga gtggattatt ctttatcggt
    14701 gtcggtctgt tcgccacagg aagcgcagta tttatttcgc cactgttgct gaaaggaaca
    14761 aaagggcttt tttatgtcat gatgaacttt gttaaatgga tgggacgacg ttttattaca
    14821 aaaagagatc tgcattcagc gggcgtttga aggaggacac ataaattgag attgaaacat
    14881 tttttgatta ccggtattgg tctgatgctt actggtggcc tcctagcagg tgcaacttat
    14941 acattaggcg ctcaaaagag tctgacttgg gataatggtc ctcggttgat cgatatggtt
    15001 actgagacaa aaaaaatcaa ggatgcaaac aaaatcgtca tccatgcaga aaatcaaaca
    15061 gtttcggtca tacgcggatt ggattttgag ataaaaacga cttatgataa gacagaaaag
    15121 ccagaaataa aggagaaaaa tggcgtgttg actgtgaatg cagtcaaaaa atcaacaggt
    15181 gcaatcatta gtttagaaga cacaagtgca caaatcacta ttgttgtccc tgcagatcat
    15241 gaattatctg aattgacaat cgaagggaat aatagtgcaa ttgatatcaa cgaagtatca
    15301 atcaaaaaag tgaatactaa gacacatgat ggctgggtat cgttcaatga atttacagga
    15361 ggaacaataa ctgcgaaaga tgaattaggc tcacttcgat tagatgaggc caatgtggat
    15421 aacttgatcg tcacaggtga aaacagcaac ttccatctag aagacagttt gatcagcaaa
    15481 gaaggaaaag tcatcctaaa cggtggcaat ttatctttgc atgagatgga aaacagtggc
    15541 tatactatcc aaacgtctgg tgaatcttat attgaaaaag actaccagcc aataacaaat
    15601 actttgttag aacaaggaca gaaaaagcta aagatcactg ctaaagacac atcgatcaat
    15661 attacgacgg atcaagagga caatgaaaca gattgatcct gtactcaact ggatatttta
    15721 agaaaaaaca gcaaagatcc ttccaatctt tgctgttttt catcgcttaa cgcacataac
    15781 tatagtaaaa taagaaagaa aatatctaaa aagaaaacag ggtgaaagag tatatgaagg
    15841 tagaagaagg tcaaagagaa aagttaaaaa cagaaatgga aagattacat acttatatca
    15901 ctcaactaag ccaaaccttc tatgatccag acaaagaaaa agtgatggtt aattacccga
    15961 acaattcaga aggtcgccaa ttagagcaag tgtaccatga agttttcaaa catttattga
    16021 cggtgaagaa agagctggat tattattctt taccgattat cgatacagga atattgaagt
    16081 atgatgggaa gaaggaacga tttatcttta aatctgttcg ggaagatttg cctctttcag
    16141 cagggatgga tctagaggtc ttggtagaag actattttac agaagaaaag cactgggttc
    16201 gaacaaaatt ggattattta ccgcaggcag ccggagggac acaggcaagc ggttggtaca
    16261 tcactgaaga caaagagtta gaactagaag gtgtaatggc gcgtattcga aaaaaaaatt
    16321 aaaaaagtaa cattagatat ttattgaagg ataaaggagc gcaggaagta gcatgagaga
    16381 aaaaagagca ttttatatca atggttatgc agggattgtc ggattggtcg tattagcttt
    16441 agttggattg tttttcttct atttaggtat gtggcaagcg aaagtttggg cacttttttt
    16501 aagtattttt ctttggttga tcacgttatt gttgttaagt tcggcaactg tagtcagtcc
    16561 taatcaagca aaagtaattt tgttttttgg acaatattta ggtacgattc gagaaaatgg
    16621 ctttttcctt acgatacctt tggcacaaaa aatgactgtt tcgttgaaag ttcggaattt
    16681 caatagttct gttttaaaag tcaatgattt ggatggaaac cccatcgaaa tctcagcagt
    16741 agtagtcttt aaagtcgttg atacagcaaa ggcattattc gatgtagcgt attatcaaga
    16801 ttttgtagaa atccaaagtg agacagccat tcggcatatc gctagccaat acccgtatga
    16861 tacattcaat gagaatgatt tgacgcttcg aggaaacacg acggcagtgt ctgacgagtt
    16921 gcaaaaagag ctgcaagagc gtttagcagt agcaggtgtc gaagtaatcg aaacacgctt
    16981 gaaccattta gcctatgcaa cagaaatcgc tagtgccatg ctccaacgtc aacaagcaaa
    17041 agccattttg tctgctcgac aaaccattgt tgaaggagct gtcacaatca ctcaaatggc
    17101 cttagagcaa atcgaagatg gacaagaaat caatttcacc gatgatcgga aagtccaatt
    17161 gatcaataat ttacttgtct caatcattac agacaaagga acgcaacctg ttatcaatac
    17221 aggcgacatg aaagaccgta agtgacatac tttttgtaag ataggaagaa ggagtttagg
    17281 acggattatt gtcttgaact ctttttttag ttgttttaaa gaaaatgaca aaattgaaag
    17341 aagaatgata gttgattcga aggcagaatt gttcgctttt ttctataatc agtatataaa
    17401 gtaagagaga aggggaaaaa gtgaaaaaac ttctgatcgg tctacttgtt ttcatcgttg
    17461 tggtacttgc aggggtgaaa atcgcagatt acgtagttat gggtggagaa gaatactatg
    17521 tacaaattac gacagatggt acaaaaacag tttcaaaatc agactctgga caagaaacag
    17581 tcaactatag atatatcctg cctggctata cgaaagaggg taaggaaaaa gaactggact
    17641 tcaagggaca attgactcga ccgcttcgta aaggagcata tttaaaaatc acctggaaca
    17701 aaaataaagg tgtcacttcg tatgaagaag tcgaaaaaaa agacgtgccg aaaacagcac
    17761 tggaaaaact aattaaaggg tagggaaaga aatgaacaaa atagtcgaag tacagaatgt
    17821 aacaaaagta tacggaaaaa acaatgagaa aaagacgcaa gcattaagcg gcatcagctt
    17881 cgatgtagaa aaaggcgaat ttattgggat catgggcgct tctggttctg gtaagtcgac
    17941 acttttgaat attttatcca cattagataa accaacagat gggcatatcc gaatcaatca
    18001 aaaagatgtg accacattaa aaggaaatca actagcagat tttcgtgcga atgagattgg
    18061 ctttattttc caagatttca atttattaga aagcttgaca gcacaagaaa atatcgctgt
    18121 tcctctttct ttacaaggcg tcagaccaaa agaaatcaag caacgtgtcc aaaaaattgc
    18181 agaacgttta tccatctctc atattttgga aagctatcct tctgagattt ccggtggaca
    18241 aaaacaacgg gtcgccgccg cacgtgcatt gatcacacaa ccaacgattc tattagggga
    18301 tgagcctaca ggtgcgctgg attccaaaag tgcccgggat cttttagata cgatggacga
    18361 attgaataca aaagatcatg tatcgattct tcttgtgacg catgatccgt tttctgcaag
    18421 ttattgccaa cgtattttat tcatcaagga tggcggtatc catcaagaag tgaaacgtgg
    18481 agatcaaagc cgtgattcct tctatcgaga aatactatct attcttggaa acttggaaca
    18541 gtagggggag attgactatg ctttggaaat tatcacttac tgggatcaag agccgtttaa
    18601 gagactactt ggtgttattc tcaggattag ttatggcatc tgctattttt tacatgtttg
    18661 aatcaatggc aagcaacgaa gcttttttga aaagtaatac gatcgttgcg gctacagtta
    18721 tcattttccg attcggttct gttttactag ggatcatcac attcgtttat attttatatg
    18781 caaattcctt tttgatgacg atgcggcaaa aagactatgc aatgtttatg atgctgggag
    18841 caaaaggcag aaaaatcgca cagatgattt tttctgaaac attcttagta gggatcgcag
    18901 ctactttagt gggttcagct gtcggtgtag gattgacaag tatcgtccac cgattattag
    18961 tggatcagtt agatctcaat atttcgcatt ttacaccttt tagtattcac gggttgttga
    19021 tcactgtttt gttttttgct gttttgtttt tattagctgc tgttgtcaat gctttttcga
    19081 ttgtcaaaaa accgatttta gcattgattc gtgcggatca gacaccaaca agaatcaagc
    19141 agcataaatt cttgtttttc cttgaagtag tactgggaat tgcttttcta ggctgcggtt
    19201 actatatgat gaaacacgtg gggacctatc aagtctttgg tataggggta gccttagtta
    19261 cgatcgtatt aggaacttat ttcattttcc attctgtgat cattttcttt ttatcattac
    19321 taaagaaatc agaacagatt tcattgaaaa aattgaacaa tttcacattg tcacagttaa
    19381 actttcgtat tcgtgaatat acacaaatgc tctcaatggt cgctatgctg tttgccttgg
    19441 cactgggcgc tttgacggta ggattaggtt ttaaaaatga aattgcagac ttgacagaaa
    19501 acaattctgg atatgatcta gtgatgaata atcctcgggc agaagatcag caaaaaatca
    19561 aagaactttc accaacactt tctgcttctt atactcaaaa agaagatgcg ggaacgattt
    19621 actataatgc aagtgaattt gcggctcaac cattgatcat gataaaacat gaagatggaa
    19681 caccgccgaa gatcacgaaa gaaaaagttc ctgctaaaaa aatgaatgaa ctgactgtac
    19741 aagaggaact tcgaagctat gaattgccgg aacagcaaga aaaagaaatc aaggtagtca
    19801 gtcaatcaga atttgatcaa ctgaacttgc cacaatccac tttacagcta gttcaggtaa
    19861 aagacttcca agcaaacctg aaaccaatcg aaacacttgt tgctcaaaat aaaatgaaca
    19921 atccatcttt gcaaaaagta gagtacagca accagaaata tgaaatgtat gaagtgtata
    19981 atggtctatt ctctggtttt gaatttatgg gattcttcct aggtttagct ttcttgacca
    20041 tgcttgctag ctgtttgatg ttcaagatct tgtcaggaag caagagtgac attgctcgtt
    20101 atgaaatgct tgaaaagata ggaacacgac gcgggttgct taaacaatcg atccgtagag
    20161 aagtaggcgt gttattctta gcaccaggaa tcttaggtgt agtccatgtc ttattcggat
    20221 tacaaatgtt tggtctattc atgcaaaatc catacaaaga tatttgggtt ccgtttacga
    20281 tattcttcgt attgtatttc atctactatg tactgacgac atggttatat actggaatcg
    20341 tattgaaaaa gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa
    20401 aaatagctcc tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    20461 caccgtttgt tcggaaaaag aggttgtgac aatatttttg tcacaacctt tttttcacgg
    20521 tcttttatta gaatatagta aattttttct cgttactcac ggaataagct gttaagaaaa
    20581 cggtttccat gtatactgga atcagtgcaa atgaaagaaa agagggtgca aggaacatgg
    20641 aaatcaatga acaaaatttt ggcgaaaact atcacttgat tacgatcacg aatcaaaatg
    20701 ggacatcttt gtctgtcagt gatcttgggg cacgggttgt tcattttcaa acaataatcg
    20761 ataatgaaaa acgagagttg atcttaggtt ttgatgaagc aaaagaatat ctagaaaaag
    20821 acccttatat cggtgcttca atcggacgta tggctggacg gattacgaat ggcacattca
    20881 aattagatga tgaaacgttc gaagtaaaaa cagacccgga cactggtcat tgtttacatg
    20941 gtggtgcacc tagctttgaa gccaagcagt ggcattatac agtcatcaat ggtgaaaacg
    21001 aagcatcggt cattttctat cagactagcc cagatatgga aaacggattt cctggaactt
    21061 tagatgtaga agtacggtat acgctgacga acgatgatac atggcgagtg acggtccaag
    21121 ggatcagtga ccgcaaaaca gtattcaatc ctacaaatca tgtctatttc aatttaaccg
    21181 gtgatccagc acagtcaatc gacgaacata tactttggtt gaatagccga ttatttgcta
    21241 cgctcaataa agatacaacg cctacaggag aagtagtttc agtaagtggc acagcatttg
    21301 acttccaacg tcctaaaaaa ttaggtgagg tctttgccag tgattttcgc caatcaatga
    21361 tagtgggcgg catcgatcat ccatttttgt tgaaaaaaca atctggttta ctaaatgctg
    21421 cggaattgac aagcccagat gaaaaagtat cgatccaagt aaaaacagat gcttcaggaa
    21481 tcgtgatttt tacagcaaat tttggagaag atggaccgga aatgcgcggg aataaattag
    21541 taaatcatgg cggtatcaca tttgagactc aagaactgcc aggagcggag cgacttgaaa
    21601 gttttggaga tgtcactctg gaacctaacc aagtacgcga gtttgtaacg gaatacaaaa
    21661 taaatataaa gaaatagaat aaattaaaaa gggagtgtga cagtgtcaca tccccttttt
    21721 aatttattct atttctttaa aggcagatca agccagaatg atttgtctaa acttaacaag
    21781 gttagtacag gaacagttgc agctttaact gtacgagtag ttgtttctgg tatctgtctt
    21841 gccgtaaaat tgttccacaa atcccagatt gaagcgacac ggaagatgga agaagcgttg
    21901 ttgacgcgtt ttatcgttga aaattctaat cctgtgattg gcacgccatc tgttgtccat
    21961 ttgattggca catcgaatcg cttagacata ttgaggattt cagcgtttgc ttcttcagtt
    22021 gttgctgctt cttttttcgg ttcatcatat tttgtcaggt cgtacgcttc gatgatcgcg
    22081 ttcaattttt gtgaatattg gcgatctgta gcataacgtc cttccaaata tttggttgct
    22141 tctttgtagt cttttgtttc cgacttccaa gttggtttgt agaaatcagg attgccagtc
    22201 agtccatttt tgatcaagtc ggcatagtct tccaaacttt ccttgtaaga aggatattta
    22261 cggaattgtg cgcggatttg gtagctttgt ccggaactat cttgttcaag tgtattgaag
    22321 gtcacgctgc ttcctttata tgatcctttg ataccaaaaa ggttatgatt tggttcgctt
    22381 gataataagc tgtttccaga tccactttca aggatagctt gagccatcat gactgatgcg
    22441 tagattcctt ctttttgccc gatttcctgt gcatcatctg caatcttttt caagaactga
    22501 tcagttgttt ggttttgact gaaatggaac gagccatcta atccatctaa aatagatgag
    22561 ccgcctgtca gcgaacctga agcagcaggt aaacctgcta agtttactcg gacagcaaaa
    22621 tcaggcgtga aatctgaaat agaagttact cgaactttat ctcctggttg aggcgcatgg
    22681 ataaattgtc cgttcccaat atagatacct acatggtgag taacaccgga agaaggtccc
    22741 cagaagacga ggtcgccagg ttgggcctga cttactggaa tctgcgtacc tgcatattgc
    22801 tgttcgcctg tccagccacc gatataatga ccggttgctt tcatatatac gtaataagtc
    22861 aaaccagaac agtcaaaagt atttggacct tttgctcccc atacatatgg ttttccaaga
    22921 tgcttgaatg cttctcgaac gattgcttgt tggacattcg ttgtatttgt aggcaataca
    22981 gcatcaattg gtgattttac aacaaaatca gaagaagcag aagatggatt ctctataccg
    23041 ctttgatttg ctgtatttgg acgagatgaa gcattgttcg ttccgccatt cgatgaacca
    23101 ttgtttggat ggtttggttc tctctgagta tttccattgt tattcgatcc agatgggttt
    23161 gtttgattcg aagaattccc gctgttcgaa ttattgttat tattgtttga attatttgaa
    23221 ccattattat tgttgttatt acccacatct ggtttgtttg gcgtttccgg atctggtgta
    23281 ctagggactt cttcactagg atcagttggt ttctctgttg aagtatccgg tccagcttta
    23341 aattctgcag taatagataa attgttcgta cctactgtaa aggtctttgc atcataattt
    23401 acttctttgg caccattaac agcgactgaa gcaatttgtt caccttcagc tggcgtaata
    23461 gtgtaagtta ccttcgtttc cgctaataaa ccttctaccg ttccactttc cgtaatatcc
    23521 aaaggttgct gttcagggga atccgctgtc gaagcagtca gttggatctt accaatcaaa
    23581 gaaggagcaa ttgtaaggga atatgtctcg gctggctttt cattttctga tggatcagcc
    23641 ggatcaattt cagttggctc ggtcgatcct gttgttgagt tagttgttcc agttgggtct
    23701 tctgtaaaag aatccgttgt ttcactagta gctgagctgg atgtagtcgt atctgttgtg
    23761 actgtttgat cagctatttg attatctacg gcaaatacgg tattggtcga caaaacagac
    23821 ggagcaatca gccaagtagc agctagcaaa cgtatcgttt ttttattttt cataataaac
    23881 tagtcctttt cctttcattg attttctgtc ttattcagca ttcattagac attttttgga
    23941 acagataatc gtgtcgaaaa gctttttctt ttcataaatc tgtctgtgta ttctaaataa
    24001 aacaatttgt aaataagcaa cgagcattca ttcgtttttc tttgacgttc attttattgc
    24061 atagaccacc tttgtttggg gataaacaag tggggataaa aatggttatg catatgtaaa
    24121 ttataccaaa gtttggaaag tatcacaaaa atttataaaa ataatgatcc taaaaagaaa
    24181 aagactgaaa tatgtgataa acatctcata cttcagtctt tttcattaac tattcataag
    24241 acattaggtc ttcatcaaac ccgctaacga gagtagtgat cattgctacg actagactaa
    24301 cagccagtcc tgctaagtac cagaaaaaag taccggaaat gaatgcaggc aatgtggtca
    24361 gactaccaaa tacaaaagcg atcgctttca cttgggcaaa tcccattaca gctccaccag
    24421 cagctgcacc aatgcaagca gcaacaaatg gttttttcaa tgggatcgca attccgtaaa
    24481 cagttggttc ggtcactccc gcaagtaaaa tcgaagcaaa agcagaaccg gagatcgaac
    24541 gggtttttga acgtttactt cgcagaaata cacctaaagc tgcaccagcc attccaaaat
    24601 tggctgctcc tgtcaacgga acgattcggt cgaacccata agcagagata ttactgatca
    24661 tgacagggtt tacagcccat tggataccaa atattaccaa aatattccaa ccaccagcga
    24721 tgattgcgcc actgatcggt ccgttaatat ccatcatcca agtaattgcg gcagctaaac
    24781 cttcgccaag gtaaacaccc gcaggcccga ataccattaa cgtcaaagga accgtgataa
    24841 ctaatgaaag taaaggtaca aatactaatt gcatattttc gcgaatatat tttttgagga
    24901 atttttcaac aaaagaataa aagaaaatac ctaaaatcgc cggcatgaca gtagacgtat
    24961 actgcatcag tactacagga atccccagaa aatcagtagc tgctttgcca ctgtctgaaa
    25021 gtaaagaggt cagattcggt tcaatcagtg ctgcagcaat gacagccgca atatatccat
    25081 tcactttaaa cgttttggca gcagaaaaag caagaaagat tggcagaaaa taaaagacgg
    25141 cattcgacgc agcagacaaa atatgataag ttccgcttgt ttcactaagc cagccaatcg
    25201 tagtcgctaa aacaaccaga ccttttaata taccagaacc ggcaaacagc ggaatgatcg
    25261 gcgtaaagat ttccgatatc aaaccaaata gtttttcgat cggatttagc ttttctttcg
    25321 gagctgaatg atcgatttga cgcgtcaatt ccttgtaagc aggagccaat ccctgatcca
    25381 tgattatttc tgcatatcta tcgagtaaaa tgaaatactt aatggaaggg atttgctgtt
    25441 ttaatttttc tgcattggct actggctgtt ttttcaattt gatacgaagc cgagtcgcac
    25501 atggaaggac agaatcaata ttgtcttttc ctcccaactg ttcaatcagc atatgaatct
    25561 gctcggacag taccatctgt tcaggattca ttgtcgtttc cattagaaga ccactccact
    25621 cactaatata atattggcgt aaggtgtcat ttcgcctgta cgaataaatg catgacagtt
    25681 attcaactct tttttcattt cttcatgtgg gataaagcta atcggtgtat ctggtaacaa
    25741 taatttgatt ttttccaata tttccgggtt cattgtcttt atttcttccg ccaattcaat
    25801 cgattgtacc tcaagttctt ctaaaacgtt ttctaaaaca gataaaaaac ttggaagacc
    25861 gtttgagaca gccaaatcta ttttttcagt accaaagggg acaggcatgc ctgcatcccc
    25921 gatacttaat ttatcaaagt gccccatttg tgcgattact cgtgaaatat ctgaattgat
    25981 tacttttcct tttttcatgt ttgtttcttc ctctcattgt taattttaga ctgctgattg
    26041 ttctaaagct tcgcggtaag ggatagaagg ttgcgcacca taagactgca cagcaacaga
    26101 agaggctaga tttccataaa ggatcgcttc tttcaagtta ctaaggtctg gctgaagtgt
    26161 gctgcttaat gcgccaataa atgtatcacc tgcggctgtt gtgtcaacgg catccacttt
    26221 aaaagcaggg acgataccgt gttctttttc tgtatgatag aaagcgccgg cactgcctag
    26281 tgtaatgatt actgttccaa tacctaattc atggagtttt tcggctgctg cgaccaaact
    26341 gttatgatct gtcactctga ttcctgtaat gatttctgtt tctgtttcat ttggtataat
    26401 gatatctgtc ttttcaagta gttcagtagg aatcgtttca cgagctggtg caggattcag
    26461 gatcgttgtt tttcctgcgt cttttgcaat agaaaaagct aggatcgtcg catctaagct
    26521 actttcaaac tgagcaataa taaagtcact gtttgtaatc agttgcttgt tttttaggac
    26581 ttcttgttca tggaaagcat tatttgcccc tgcatggatc aagatactat tttctcctgc
    26641 tgcgtctacc ataatcattg cttgtccggt tgtggcgcct tggatttcag ctatcccaga
    26701 tgtatcgatg ttttcttttg tcagtagatc taataactgt tgtccttcgc tgtcagcccc
    26761 aacgcctcca ataaaagatg tccgtgcgcc tgaacgtttg gctgccactg cttggttggc
    26821 acctttacct ccgccagcgt gaaaaatctc atgcgcatgc atcgtttcgc ctggcttggg
    26881 catgttggtt aaacgaaggg tagtgtccat attaatactt ccaattactg taaccgtgtt
    26941 cattttacaa acctccaact atctcattta ttttgatggg aaaattattt tttaaaacgt
    27001 tttagtaaaa cgttttaata gttcgataaa aatatagcac accttgaaag ctatttcaag
    27061 ggtgcagttg aaaatctttt ttctaatcgt acaggtaatc ttttttcttc ccattctttt
    27121 tctgggaatt ggatccgatc taacaataac ttggcagatg tttgtcccaa ttcgaagatt
    27181 ggttgtgcga tcgttgtcag tttcggcttg atatactcgc acatatctat attatcatag
    27241 ccaataatgc tgtagtcttc agggatgctt tttccagctt cctctaatcc gcgatataat
    27301 ccaaaagcta attcatcatt taaagcaaac acaccagttg cagcagaatc aagaagttct
    27361 gctgtgatct ggtagccacc ttgttttgag aactgtgtag gtaagaggat cagttggtcg
    27421 tgagggattt ggtaaacatc aagagcagat ttaaacccct caatccgagc gtggacattt
    27481 tccggtggat tttcaggata gacaagggcg atcgtttgat gtcctagact aagcaagtgc
    27541 atgcccgcta ggtaaccacc gcgaaaatcg tctgttcgta cggcatcact aaatccttca
    27601 gattttttct gatccaaaac aatgaagggc cggccttgtt ttttcaaatt ttcgttgatt
    27661 gcatctgtag agacagcaga agtcgcgata atgaatccat ccacacctcg acgtgtgagc
    27721 tcggctagat attcgatttc tttttgatga tcagaatcag cattacataa tatcgtgacg
    27781 aaattctgct tatacaagat atcctcaatt cctcgcatca gtgtactgaa gaatggattc
    27841 gtgatatcag gaacgagtac accaattgtt ttcgtttcct tggtaatcat ttgtctagca
    27901 aaatagtcag gctgatagcc taattcttct ttggcagcta aaactttctg aacagttttc
    27961 ggactgaatt tctccgtatt tccatttaat atcaaggaga cagttgtgat tgaaacgccg
    28021 gaaagtttag cgacttcttt gattgtgatc ttttttttca ccatgttcat actcctatct
    28081 aaatcctgtg gttattttcc catgaaatca aaaagaagta aatgaaaatc acgtgtgcca
    28141 gtacttattt gctttgttgg atagcttcga ttatttgttt cgttgagcgt actcgtgcag
    28201 ttttcggaaa tatctttttt atgaacaatt gatgggattc tttgtcacga tcactgcaag
    28261 catcttcagc aagcacgata tggtacccgt gttgaaaggc atctaatgca gtagcatata
    28321 caccgtttgt tgtcgctaca ccgcttaaaa tgatagtttc gattccacgc cggcgaagtt
    28381 gaagatcaag agaagtacca aaaaaagcac ttggattgta ttttgtgata gtcaacatat
    28441 tatctgtatc tttcaaagaa tccttcaaaa gtaaatcagt gaattctgga ggtacagaag
    28501 cagtcattcc acccatatca gtttgttgac tcaaataagg gaaagtggag atgtctacgt
    28561 tcactaaaac tttcaaggca gcggtacgtt tcaatgttct tactaacaaa tcattgtttg
    28621 tcagaataga tgctgcagtg tgtggagaga gtgtaccgtt atttacgatc tctttttgca
    28681 tgtcgatcgc gacaaaagca gtttttgata aatcaaaaat catattttcc tttaaatcat
    28741 tcatgatgtt ccttctttca aataacaatg ttttttatgt aagtaaatca taaatcgttt
    28801 atttgtcaac taaattaaag gattcctttc tttagtctag agaaattcag tggtaaaata
    28861 aataagtgtg agaaaacgca ttcgatgcat agatatagcg agaaatatcg tgaaaatgga
    28921 ggaatcagag tgaagtatgt cgaagtaaaa aatgaataca aacgatacaa aatgggagaa
    28981 acgaccatta cagcaaatga cgatatttct ttttcaattg aaaaaggcga attagtgatt
    29041 atacttggtc caagtggagc aggaaagtca acgattctta atattttagg tgggatggat
    29101 acacctgatg aaggtcagat cattattgat gacacagata ttgcgcaatt ttcagataaa
    29161 cagcttactg cttaccgacg aacagatgta gggttcgttt tccaattcta caatctcgtt
    29221 cctaatctaa cagcaaaaga aaatgtggaa ttggcaacgg aagtttctcc tgatgcgctt
    29281 gatcctgtag aggtattgag gcaagtgggc ctagctcatc gattgaataa ctttccgtcc
    29341 caattatcag gaggcgaaca gcagagggtt tcaattgctc gtgcactagc taaaaatccc
    29401 aaacttcttt tatgtgatga accgacaggc gcgctggatt ttgaaacggg caagcaagta
    29461 ttgaaattat tgcaaaacgc tagcagacaa cacggcaata ccgttttgat cattacacat
    29521 aattctgctt tagcgcccat cgctgataga gtcattcaca ttaatgatgc taaagtaagg
    29581 tctgttgaat taaatgatca tccttcatcc attgatgaga ttgtatggta gggggatttt
    29641 atgaaaacaa gaacatatct taaagcaggt tttcgcgaaa ttcggcaatc taaaggacga
    29701 ttcattgcaa tcatccttat tgtcatgcta ggaacacttt tatttgtagg tgtcaaaact
    29761 gctggacctg tcatgcaaaa aacgatggat cactatgttg gtacagcagg actttctgat
    29821 ttgcaaatcg tttctactgg tggcttgaca caaaaagata ttgctcaagc agaaaagatc
    29881 ccagatgcaa aagtagaaac aggaaaacag atctattatt ccaatactgg caaaaacgaa
    29941 gtgattcaaa tattttctta taacaaaaat aacaagcaga acaagctaca attaactgat
    30001 ggaaaactcc cggaaaaacc aaaccagctt gtattggatg aaaaagcgga agatgaaggg
    30061 tataaaatcg gtgatattta tcagattgat tcagatgaat tgaaagaaaa agagtatacc
    30121 atcacgggct ttgtccgttc tccattgttc atcaacaatt tggagagagg atacgcgaac
    30181 gtcggaaatg gtagcgtcga ttattttgtc tatttacctg aatccgtttt taaaacagat
    30241 atttattctg tcttatatct tgattttaca aacgtaaaga atttgaatac gtacagcaaa
    30301 gcttacaaag ataaaatgga gcaaaatcaa gaaaaggcag aaaagtattt acaagatcga
    30361 cccgaagaac gcttagaaga gctgaaaaaa tcggccaacg aagaattaga accggcacag
    30421 caaaaaatag cggatggcaa agcacaaaca acgcaagcaa gaacacggtt agaagatgct
    30481 aaaaaacagt tagcgcaaca gtcagcaact atccagcagc ttcctgaagc gcaacgaaca
    30541 atcgctgttc aaacattatc tcaacaagaa gctcaacttg ctgaacaaga aaggcaatta
    30601 gttgagaaag aacgtgaact ggcctctgca gaggaagaat tgaatcgaaa tgcagaaaaa
    30661 attgaaaatt taactaaacc atcataccta tacaatgaac gttcagaaaa tgtcggtttt
    30721 caagaatttg gtgatctgtc tgatcgtatc gctgcgattg ccaatgtttt tcctgttttc
    30781 tttttcttta ttgccgcatt gatcaccttt acgacaatga cacgtatggt agaagaaaac
    30841 cgcagggaaa tcgggacatt aaaggcgtta ggttatacga aagttgaaat cgcaaaaaaa
    30901 tatgcaatct atgcaagtct agcctcaggg ataggtattg ttttaggaac gatattagga
    30961 acgaatcttt tgccaaggat catttttgag ctgtctaacg agcggtatga tattgggagt
    31021 gcgcttattt tttatgactg gccgccgatt ttacaagcag ctgccgcatt cttcattgcc
    31081 gcatttggcg ctgcaatgat cgttttattc aaagatttaa gagaacggcc tgctgcattg
    31141 cttcaaccga aagcaccgaa gccagggaaa cgcatttttc ttgaatacat cactccgctg
    31201 tggtcacgcc ttagctttaa tcaaaaaatc agttaccgaa atctcttccg atataagtca
    31261 agaatgctta tggcgattat cggaattgct ggatgtgccg ggctgatggt tgcaggcgtg
    31321 ggattgaaag attctctaag ttctgtttca gccaaacaat ttggaccgat cattgactat
    31381 caagcaatcg ttacattgga ttcatccacg gaagatagtc gtcaaaaagc agcggatatg
    31441 ctgcagaagc aagaaaaagt aaatgagatc ctagctattc atactcaaac cgtagaattg
    31501 agaaaaaaag gacatgcttc gcaaagtctg acagtgatcg taccaagtaa aacagctgat
    31561 ttggatcgtt ttatccattt aactgatctt gaagagaacc cacgctcctt gcctaaaaca
    31621 ggaatcgcta ttacgcaaaa agtagcagaa ctatttgact tatctacagg agatcatatt
    31681 tcgctttatg atgaagatca aaaagaatgg aaagtcgaag taaaagaaat cgtgaaaaac
    31741 tatctaggta gctttattta tatgtcccct gaatattatg cttcagttac tgatcaatcg
    31801 atgatggata atgcattttt gctgcgtaca gataagatga caacaaaaga agaagaaatg
    31861 ctttccgaga aactgcttgc cacagacgct gtgacaaata cgtctttcat gtccaaacaa
    31921 atcgaaacgc aagaagaatc aatggcgaac ttagactcgg ttgtcttaat ctttgtcgta
    31981 ttatcaggac tgctggcttt tatcgtcttg tataatctaa ctaatatcaa tatttccgag
    32041 cgtgtacgcg aattatctac aataaaagtc cttggatttt ttgataaaga agtcacgatg
    32101 tacattgttc gagaaaatat catctttact cttttaggaa tcatcggagg atttggtgta
    32161 ggatatatat tgactgactt catcttgcaa caagcgtcta tggagaatgt catctttcca
    32221 ttagtgatca catggtgggc ttatgtctta tcagcaggat taactatcgt atttacggtt
    32281 attgtgatga ttgttaccca tttcaaatta aagcatatcg atatgattga tgcgttgaaa
    32341 tcgaatgaat aaaaacagag gttgtgaaat cagcggtttg acctgagtat tagagaaaca
    32401 aacggcaaaa tagtttttca tattttgccg ttttttgagc taataccgaa ggacagctga
    32461 ttgaacacca tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact
    32521 caaataaaat tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc
    32581 ttttgaacac catttataga agaaacaaga atattttgct attttaaaca agaggataga
    32641 caaatacatg tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag
    32701 ataaatttta caaacgcttt cggttcagtt ataataataa atgataactt gtatatataa
    32761 gttgtggtag gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa
    32821 gtcatttacc aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat
    32881 ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg
    32941 ctaaacccta tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca
    33001 gccatcgatc ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag
    33061 aaataccgga tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa
    33121 tggttcaaaa aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa
    33181 gagccaacgg attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg
    33241 gggaaatatt atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa
    33301 gcagtaagag aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga
    33361 ttccgtttcg atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa
    33421 aatgatggca aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg
    33481 aaccaagcaa cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg
    33541 acgatcgaaa attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt
    33601 aatttccatc atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt
    33661 gattttgcag aattaaagcg actatttcat acatggggag aaaagatgag tgagggtaac
    33721 ggctggaatg ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta
    33781 gctatcgatc agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac
    33841 cgtgggacgc cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca
    33901 tcgatttccg actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa
    33961 ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga
    34021 acacccatgc aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta
    34081 aaagttggag gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct
    34141 attttgtcct attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa
    34201 ggagattatc atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt
    34261 gaaggacagc agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc
    34321 ccagaaggat tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat
    34381 caaagtctgg agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac
    34441 acaaaacgtc gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa
    34501 taatcttctt cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg
    34561 tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata
    34621 ggaataccaa aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt
    34681 aagatcgtaa tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca
    34741 ccgtggagaa gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag
    34801 tcttggaatg caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg
    34861 atacataaca acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag
    34921 aaacaaaccc agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat
    34981 gaacccaatt gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt
    35041 gggacaatcg ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg
    35101 acaattggac cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg
    35161 atccgttgtg cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg
    35221 tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat
    35281 tgtcggagac tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt
    35341 gcctgttctt caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata
    35401 atcacataaa cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg
    35461 actggttcca aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg
    35521 ataatgattc cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc
    35581 gcaagtaaga taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc
    35641 aaacgattcg ttgtatctgt ttcttttttc actctttttc ctcctttatc tataacttca
    35701 ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca
    35761 atcattcata taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac
    35821 catatgataa attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat
    35881 cggaacattc aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg
    35941 tatattggcc atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc
    36001 cgaggacagt aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac
    36061 aaaagcaatc gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc
    36121 ccacgattat gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt
    36181 ttataatgat gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg
    36241 caatgtccat acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg
    36301 acatgatcat gaccatgaac acggggaaga aggccataca catgagctag acccacatgt
    36361 atggctagct ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga
    36421 agcttaccca gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact
    36481 tcaagcactc catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt
    36541 cacgcaacat gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat
    36601 tgctggtctt tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt
    36661 tgttaaagaa catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc
    36721 cagaacgtta gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt
    36781 gacgaatgaa caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga
    36841 agcgttgaaa aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa
    36901 agaaaagagc gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc
    36961 agattgggca ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc
    37021 tgtttttgaa tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata
    37081 ttatacgaag ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc
    37141 atttacgttt aagaatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat
    37201 cttgacctac gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga
    37261 aagtggcgct tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga
    37321 acattttcat ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa
    37381 ttggccaaca ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt
    37441 gcactgatag gcaaaaaaga accgaagggt ttggatcggc tggtcattta gtcggtccaa
    37501 actattcggt tctttttatg aaatcaattt ctttaatcca cggatagagg ctgggatggt
    37561 ctcgccgttt gccaacgtga cttctctggt ttttttatta atcgaccgca cattttgtgt
    37621 attgataaca taggatttat gacaacggat aaaattccga tgcattttct cgatttcttt
    37681 gatcttctca tagaattgta attgggcatg agtcaaatgg acttccagtt tatgtggcgc
    37741 tcctagcgaa gtagaaaaat attggatgtc tttgatcggt aaaaatttga tctcatcatt
    37801 aaactctatt ttgaagatat ctttttggat ttgggacatt tgaaaatctt cgaaaatttt
    37861 gctcaggata tgctcgacat tttcttttaa atcataaaga tcttctttga cgatataatc
    37921 agtcggttcg atgttgcttt ttaagatttt catcgacatt tccgtatggg aagtcacata
    37981 gattattttt cctaaaggat ctaattcacg gatcaattta ccgatcgcta caccatccat
    38041 ttcactgtct tctagttcga tatctaaaaa gtaaatacca aaagaagaag ttctgatctt
    38101 ggctgcattg agaagttctt ccggatcaga ggtagaaata tggacataac tgtctaagtt
    38161 ttcaatcatg attctatttt tgatcaccgt tgataaaact tctaattgtt ttggatcgtc
    38221 ttcacaaata taaatgggaa tcatatcatt actcctttat tttatttgta tataaatcgt
    38281 ttgagaaaaa cgctggtttt caattgatgt ttcaaggaaa agataaggat tggtctttgt
    38341 caattcatcc aagataaaca agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa
    38401 ccctttttgt ttcaactggt ttaacggtaa tgtatttgtt gttgtattga tcacacgaat
    38461 catcacggaa tgattcttct tgagtatggt caattcgatt tttttcttct ttgcagcact
    38521 agctccttca atcgcattgt ccaagataat accaagcata cgtacaaggt ttaccgtatg
    38581 tttcgtatcc agttcgattg gctcaggaaa atgaagctgg acgatcaacc ctttttcctg
    38641 tgccatcatg agttttaatg acagtaaact acggatctca ggaactttca tattatccaa
    38701 catacttaga ttggccggaa aaaggttcat catctggcgt gtaggtaata tcgtttgatc
    38761 ataataagac cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag
    38821 cagattttgg taatcatgtt tgaattctct caagtcttga tagttttttt ctagatcttt
    38881 gatataatcc tgttgcatcc tgacttgttg ctctaataac tgatcagcta atttcttatt
    38941 agagattttc caataataaa ccaagatgat caggccaaca cacagatata gaatgaaaaa
    39001 actcgtattc aaagctaaga catcaggtgt ttcaccagaa aaacgggtat aaaaaataat
    39061 gatatagtat gtaaataata tcaatgtccc gatcgtaccg atcatacggc ggtccacagt
    39121 gtgaaaccaa gtctctaacg atttttttaa acaaaaagct aataaagcag ataaaaaagc
    39181 ggataagaca atgtgaaaat actgcaatga atctcctggt tcaataaccg aagatttcaa
    39241 aacaaaaaaa tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca ataacccaaa
    39301 aggaagcgcg taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag
    39361 cattcctata gcaattagag cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa
    39421 aatacaagaa atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat
    39481 caataagata caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa
    39541 gtacagctcc cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt
    39601 tttcagggat tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag
    39661 ataagttagt taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc
    39721 aagtagtttg taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag
    39781 gtagaaaagt aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg
    39841 atcgccttct ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct
    39901 cttgatccat agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg
    39961 aaggagatag tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt
    40021 gtccacagca gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg
    40081 cagatataag aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag
    40141 actagaacga ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact
    40201 tggacataca ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg
    40261 ttcacaactt tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa
    40321 taaaaaacgt cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct
    40381 aaaaaaatct tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag
    40441 tactttttat gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt
    40501 ataaaaggtg atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca
    40561 tatgcattta aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat
    40621 gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat
    40681 aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata
    40741 aaatgttgag agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata
    40801 atgttaaaaa acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag
    40861 gagaatactg tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt
    40921 catgaatagg agttactttt ggctactttt agagaagtag cttttaagga gcagataaag
    40981 tcaaggcttt tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa
    41041 ggatgccgat aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa
    41101 ctgaaatgct tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg
    41161 aagtattgcc gctattttat tctaatccag aaaagtatac ttttttatta cagattttct
    41221 ttttgaacaa acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag
    41281 accgctccat ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt
    41341 ctgaagtaga agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag
    41401 aaatttcgcc acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta
    41461 tgttagcgag aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac
    41521 tctattccta ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata
    41581 tatgtcccaa aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg
    41641 gagaacaaat cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg
    41701 cccagtcgat ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat
    41761 aatataaaaa ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct
    41821 tagttttttt gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata
    41881 aatgaaatgt ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat
    41941 gggcgccaaa cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa
    42001 cgacacttag atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat
    42061 catataagtt cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac
    42121 gcccgttgat tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg
    42181 ttactaatgc gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt
    42241 cataatatag aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta
    42301 gatactttgt tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa
    42361 cacttagcca ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag
    42421 cagaggcaaa agcaccatat tggatataag tataactgtc catggctcct ccgataaatg
    42481 tcagcaaaag tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct
    42541 tttatgaata tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt
    42601 tcagaaaact aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa
    42661 agaaaaaata gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga
    42721 aagatgggaa ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat
    42781 tgaaacgctg ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa
    42841 tcattgctac ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac
    42901 aagtaaaaga aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata
    42961 atcgcttcaa tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa
    43021 tcatagcagg agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga
    43081 tgacgcaaaa acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg
    43141 caaatgaact aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg
    43201 gaacattcca tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag
    43261 tagcaatgca aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg
    43321 ttttgcaaca gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa
    43381 cgattgcttc tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc
    43441 agttagttaa agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac
    43501 gactgtatgg attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt
    43561 tcaatgatgt actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata
    43621 tcggccaaaa atatgattat ctattcattg atgaatttca agatactaat ccacttcaat
    43681 gggaaatcgt gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg
    43741 atgatcagag tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa
    43801 aagaatatcc taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg
    43861 tccaaggagc aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga
    43921 ttccagcaca aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag
    43981 aaagtcagtg gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg
    44041 aatcaatcat attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga
    44101 aaacgaatat tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca
    44161 agtctttcca acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa
    44221 agaaaaatca ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa
    44281 aaagtgaatg cgatcaatac ttttctccta aatatcaaaa tatagccgtt gcagattata
    44341 ttttgtcgat aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag
    44401 cgttgcgtaa aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc
    44461 tgccacgtat agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa
    44521 agcagcatgc tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta
    44581 aagaattaaa aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc
    44641 agagcattca caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg
    44701 aaggaagttt gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa
    44761 aagcagaacc agtgacgacg atagaagaag aacgaagact attttatgtt gcgatgacta
    44821 gagcaagaaa tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag
    44881 tatcgagatt tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga tagaagagag
    44941 ggtgtgacag aagtggtcag tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca
    45001 aatttttgtg aaatttcagc ttattttcga agaagctatt tctggaaca
//
LOCUS       DADXTG010000007        43588 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.007, whole genome shotgun sequence.
ACCESSION   DADXTG010000007 DADXTG010000000
VERSION     DADXTG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.007"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            158..838
                     /locus_tag="IWI57_000300"
     CDS             158..838
                     /locus_tag="IWI57_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBC2448470.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            928..1962
                     /locus_tag="IWI57_000301"
     CDS             928..1962
                     /locus_tag="IWI57_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBC2448471.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1976..2311
                     /locus_tag="IWI57_000302"
     CDS             1976..2311
                     /locus_tag="IWI57_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448472.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2295..4775
                     /locus_tag="IWI57_000303"
     CDS             2295..4775
                     /locus_tag="IWI57_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448473.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4831..6390
                     /locus_tag="IWI57_000304"
     CDS             4831..6390
                     /locus_tag="IWI57_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2448474.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6387..7751
                     /locus_tag="IWI57_000305"
     CDS             6387..7751
                     /locus_tag="IWI57_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2448475.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7922..8992
                     /locus_tag="IWI57_000306"
     CDS             7922..8992
                     /locus_tag="IWI57_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448476.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9007..9675
                     /locus_tag="IWI57_000307"
     CDS             9007..9675
                     /locus_tag="IWI57_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448477.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9695..10273
                     /locus_tag="IWI57_000308"
     CDS             9695..10273
                     /locus_tag="IWI57_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2448478.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10674..11585
                     /locus_tag="IWI57_000309"
     CDS             10674..11585
                     /locus_tag="IWI57_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBC2448479.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11963..12880)
                     /locus_tag="IWI57_000310"
     CDS             complement(11963..12880)
                     /locus_tag="IWI57_000310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBC2448480.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13082..13519
                     /gene="zapA"
                     /locus_tag="IWI57_000311"
     CDS             13082..13519
                     /gene="zapA"
                     /locus_tag="IWI57_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBC2448481.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13577..14125
                     /locus_tag="IWI57_000312"
     CDS             13577..14125
                     /locus_tag="IWI57_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBC2448482.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14240..16600
                     /locus_tag="IWI57_000313"
     CDS             14240..16600
                     /locus_tag="IWI57_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBC2448483.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16687..17001
                     /gene="trxA"
                     /locus_tag="IWI57_000314"
     CDS             16687..17001
                     /gene="trxA"
                     /locus_tag="IWI57_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBC2448484.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17183..18961
                     /gene="uvrC"
                     /locus_tag="IWI57_000315"
     CDS             17183..18961
                     /gene="uvrC"
                     /locus_tag="IWI57_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBC2448485.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19106..20005
                     /locus_tag="IWI57_000316"
     CDS             19106..20005
                     /locus_tag="IWI57_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448486.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19998..21260
                     /locus_tag="IWI57_000317"
     CDS             19998..21260
                     /locus_tag="IWI57_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448487.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21421..23319
                     /locus_tag="IWI57_000318"
     CDS             21421..23319
                     /locus_tag="IWI57_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBC2448488.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23649..24368)
                     /locus_tag="IWI57_000319"
     CDS             complement(23649..24368)
                     /locus_tag="IWI57_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBC2448489.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24705..25037
                     /locus_tag="IWI57_000320"
     CDS             24705..25037
                     /locus_tag="IWI57_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448490.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25024..27027
                     /locus_tag="IWI57_000321"
     CDS             25024..27027
                     /locus_tag="IWI57_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBC2448491.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27046..27516
                     /locus_tag="IWI57_000322"
     CDS             27046..27516
                     /locus_tag="IWI57_000322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBC2448492.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27537..28121
                     /locus_tag="IWI57_000323"
     CDS             27537..28121
                     /locus_tag="IWI57_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBC2448493.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28136..29122
                     /locus_tag="IWI57_000324"
     CDS             28136..29122
                     /locus_tag="IWI57_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBC2448494.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29112..29423
                     /locus_tag="IWI57_000325"
     CDS             29112..29423
                     /locus_tag="IWI57_000325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBC2448495.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29445..31226
                     /locus_tag="IWI57_000326"
     CDS             29445..31226
                     /locus_tag="IWI57_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBC2448496.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31219..32595
                     /locus_tag="IWI57_000327"
     CDS             31219..32595
                     /locus_tag="IWI57_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBC2448497.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32602..33237
                     /locus_tag="IWI57_000328"
     CDS             32602..33237
                     /locus_tag="IWI57_000328"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBC2448498.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33267..34622
                     /locus_tag="IWI57_000329"
     CDS             33267..34622
                     /locus_tag="IWI57_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBC2448499.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34814..36208)
                     /locus_tag="IWI57_000330"
     CDS             complement(34814..36208)
                     /locus_tag="IWI57_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBC2448500.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36300..37271
                     /locus_tag="IWI57_000331"
     CDS             36300..37271
                     /locus_tag="IWI57_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBC2448501.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37268..38050
                     /locus_tag="IWI57_000332"
     CDS             37268..38050
                     /locus_tag="IWI57_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBC2448502.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38219..38590)
                     /locus_tag="IWI57_000333"
     CDS             complement(38219..38590)
                     /locus_tag="IWI57_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2448503.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38724..39167
                     /locus_tag="IWI57_000334"
     CDS             38724..39167
                     /locus_tag="IWI57_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBC2448504.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39210..40019)
                     /gene="yidA"
                     /locus_tag="IWI57_000335"
     CDS             complement(39210..40019)
                     /gene="yidA"
                     /locus_tag="IWI57_000335"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBC2448505.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40038..41405)
                     /locus_tag="IWI57_000336"
     CDS             complement(40038..41405)
                     /locus_tag="IWI57_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2448506.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41389..42219)
                     /locus_tag="IWI57_000337"
     CDS             complement(41389..42219)
                     /locus_tag="IWI57_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBC2448507.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42382..42819
                     /locus_tag="IWI57_000338"
     CDS             42382..42819
                     /locus_tag="IWI57_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBC2448508.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42895..43515
                     /locus_tag="IWI57_000339"
     CDS             42895..43515
                     /locus_tag="IWI57_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBC2448509.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14279 a         7487 c         9251 g        12571 t
ORIGIN      
        1 aataaaaaag gaagaatatt catgtaaacg attttattgt ttttttctct tttatacaaa
       61 aagggtcata atttcaagta agcggatgaa ttcatgagga ttttttgcat actgtctcta
      121 ctatacttag gtcactaaga aaaaggagtg atttattatg aaaaagaaca tgattgggac
      181 attattactt tcaacacttc ttctaggcgg cttggcaaca ccagcttttg ctgagggtca
      241 agcgacttca aaaggagata ttacctttac tgaaccaaca aataccgtgg agcctcttaa
      301 ccctactgat ccttcaaaac cagtagagcc agcagatccg gaaaatccag caactggcca
      361 gacaggtcct ttaacactgg atgtagttcc cgaattgcca tttggtacac acgagattga
      421 aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac gatacacctt atctccaagt
      481 atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa gggtggaacg tgacagtttc
      541 ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc gctgaattag attttggaac
      601 atccactgtt aaaagtacgt cagataacga atccactgga ccaactagtc aaactgtaac
      661 tgggttgagt aaagcaagtg cagcgacacc aatatttaca gcagcaaaag atcaaggtct
      721 gggaacatgg ctttctgttt atgatccagc aaatatcaca ctgaaagtgc cgaaagcagc
      781 agctggaaca ttcacggctg atttgacttg gaacctcgtg gctggcccag tagcatagag
      841 tatgaaataa attcaacaaa ctgcggtgaa aatactatag gtgaaggatt ttttaccgca
      901 gttgttgttg aaaggatgtg gtggacaatg aatcaaagta aacgactgtt tgtttcgttt
      961 ttttctattt taagtctatt tttcattttt ccgtcaatca gccaagcaaa ggattcagca
     1021 ggagactttg gaatcaaacc ggtatttcca gagaaccaaa tagataaagc aatcggttat
     1081 tttgatttat tagtggcacc ggaacaaaat cagacactag aagtaattat cagcaattct
     1141 tcagatgaag aacgaacatt tgaggtctct gtcaatccgg cggtaacgag tgatggtgga
     1201 acgatcgatt atagtcaaaa aaacccaacg ttagatgaga cattgccgtt tgatgtccgt
     1261 gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag cacatgcaga aacaactgta
     1321 cctatagaag tgaaaatccc agcaaaaagc tttaagggac gtgtattagc gggtattcat
     1381 gtttcaccta aagaagaagc tgaaacagaa aatgctacag aaggagcaca aatcaaaaat
     1441 cgtattgctt ataatctcgc agtcgtttta caagaaagtc aagagacgat agaaccggat
     1501 ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa agccaactgt tcagcttcgt
     1561 ttccaaaatc ctcagcctag gattatcagc aatttgatat ttacgagcaa gatcttttat
     1621 gaaaatcagc tttatattga aaatacgtct aatgcatttt tagttgcacc aaattcgaat
     1681 tttcacttga atcttgattt agctggtgat aaagcaaaag ccggcgatta ccgcgcagaa
     1741 atcatagcaa aatcaggcga cagtaatgaa tggcgattta cacaaaactt tacgatcaaa
     1801 aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg cagtacaaga acaatctttc
     1861 ccatggcttt atgcaggatt ggttgctgca gttctagtgt tatggttgat tttgctgttt
     1921 ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat gatgaggtga tttcgttgaa
     1981 aacatcgatc ttattgggag ggattattgc aagtttgctg ttcttcagct ggcaagagcc
     2041 agtccaagca gataccaaaa gttcaggtac gattacattt tatgatctaa gtccaattcc
     2101 gcatccaagt ggttcagtca atggcgcatc aaaacagcag acacagctac cgatttataa
     2161 agaatctaat caacatggcg tattcccgga gacgaatgaa caaaagatcc gttttcttta
     2221 tttgatcatt ggtatagaat tatggcttct tgcgtggttg ttgttatgga gaagaaagga
     2281 aaaggaggat gaacatgttg ctacaaaata aattttcatt ttttcgctcc ttctttttta
     2341 cttcgctttt tgttttaagt atatttatct tttcaccttc ggcagaagct gttggagcaa
     2401 cgcctcctgc catttcttat gatccataca cgtatgaaaa tatcgtacct ccaaatggtg
     2461 gacgtagtgc agtcaatggc gggaagcttg tccaactaac tggggctaac caattgccta
     2521 aattgactgc tacttatttg aacgatggtt caaatgatcc ttggaaaggt ggcacggtcc
     2581 gttttcattg ggaaagccat gtgaaactaa caaatgtact tgatttggtg aatatcactg
     2641 cacaaggatt tgcatttgtg gcgatcatca atttgccgga acatgtatca agcagtgatg
     2701 tattaaaagc aattgactgg aacaacgcct acttgtatat tgccggtgcc cccatcaagg
     2761 ttactagcgg aagtttatta tcagcaggaa gccatacctt gcgttttggg atggggtcgt
     2821 gggatacaaa tactctattg acagatatta ttgctattat tacgcgaggc gaattcaacg
     2881 tggacgatat cccgcttcaa tttgatgtta cagctaatgt cgcgaagatg acagaaaacg
     2941 gagataaata tgatacgagt ccaaacaaaa ttttgaccaa gggtaaatta caaccttcta
     3001 tgtctaaaaa ggccgttttt tctgttgatt tttatgattc gaataatatt atccaagatc
     3061 gtatgttcaa ttcgatttta gctccagctg caggtagatt ttatcctaaa ttaactggcg
     3121 gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc agagataaat acgtggaata
     3181 gttacatctc accaagtgat tcaacaggaa aatataatac atttgctgat accacagaat
     3241 acgtggatgg aaataaccag aaccttccag gctctaccaa tagtcggaca ttgacgatgg
     3301 ggctgactga aggacgtttt gatcagttgc catttagtcg ctttgatcgc gtagtcaact
     3361 attttacagg aaaaaacgtt acacaaggag caatactgac tcatactcca gcaagcacaa
     3421 gtggattgaa tcagacagcc aatatcgtgt acagtgggaa agatgcgcaa ggcggattgc
     3481 tttctcctgt tgctttaaag gtattggagc agtacaagct agacggaacg gttttaccaa
     3541 caaacttaac agccgatagt cagtggggaa agctcctttc ttctgcatcg taccaagtca
     3601 cggccaattg gtcagcaact gaactaaaaa agggttcgat ccattacagg ctttattcga
     3661 aaggaaccca aaaattagcg acaggatgga cagatcaact tttccaaacg atccaatcaa
     3721 ataacggggg tgtaaataca gctacttcta cactacctgc attggcttca gggcaatatt
     3781 attttgatta tcgcttggtg gacgatgtac ttactaacat gtatccgtct tttgcgtaca
     3841 aatggcagtc tgaacaagct ggaatcacca ctcttcctca aataacagtg gctaattttc
     3901 catctgtgag tgcagatagt cagctaaaaa accttagcag acaacctgag acaggagatc
     3961 ccctgtctgc tctatcagga gatacgattc aagaaaatgt gacattcacg ttaacaaaat
     4021 ctggggatgc gataaccgac aaaaaaatca cgatctcact tcctgaaaat actacttata
     4081 tcaatggttc cttaaaatta aatggtacaa ctatcgcaga taccggcatt caacaaggag
     4141 tttctgtccc agcagatcta ttgagcaaaa taggagatac cattcatctt acttacaact
     4201 atcaattaaa tactgtagat acttctttac aatccgtcag catactgaca aaggcagctg
     4261 tactttccag caatattaca ttagcagatg gtgctaagct tcctaaccca gttgtccaaa
     4321 catctgctaa aacaattctt gtccccaaac aagaattaac attagttaat gtaccagatg
     4381 attttacatt tggtaacgat ctccccaaac cgttgaagac aagttattat gaatcaaaag
     4441 gcgatttttc ttttgatgtg cgggatactc ggcttccaag cacgtctcct tggcaactga
     4501 caggtacatt gacttcttta tttaagaatg atcaaggtca agaattaagc ggaacaaaat
     4561 tatacttcaa tcattctgga tcaaagcaat tgattcagca aggtcaaaac acgttgattt
     4621 atgagagtga tggaacagct aaaggagagg ttctcgttga ctttcccgat accgatggtt
     4681 tactcttaga ggtaaatagc agtacgaatg ctcaacctgg cgcaacgtat caaggcatgg
     4741 tcacatggga actgacagct gggccaacca gctaagtgat tagaatttgt atggatctgt
     4801 gataactgtc acagatcctc tttttcaagg atgagaagga aacttctgct gttaaaatca
     4861 gaaatgcttg aaggagtgga cttggaactt ttcagtttgc ttgaaaaaaa tgaacaacgg
     4921 caaatcaaac ttttccaact tttattagag gaaaaaggag aggtttccgt taataacgta
     4981 cttacccgct tatcagttga tcgggctacc ttgaaagaag atctgtttta tttacaagct
     5041 aacttggaaa atatcaaaca attggaaatt gttatccaag aagggaatat tttattaatc
     5101 agacatggaa atatagcagc agtcgatgtt tattatcatt gttacttgcg ctattctaca
     5161 aaatatcaga tacttcttta tctgttggaa aatggctcat ttgatcggca gaaacttgtc
     5221 atggaactta atatcagttc ggctactttg tctcgcagag tgaaggaatt aaatgattct
     5281 ttaaatgagt ttcatctgaa aataaaaagc gggcggctga ccggtccaga aagtcaaatt
     5341 cgttattttt atttccaatt attttggttt ggacgtccct atcttgaaaa caaaaaagaa
     5401 tacagtgatt tatcaggaaa cactttttta gatctgctgc aaaaagagtt tagttttgct
     5461 attacagaag aaggacgatt gaaatttgaa atatggcttt ccattatgaa gaagcgaatg
     5521 cgctgtacga aaaaatccaa accagattat agcaatcttc ctgttggatt gtttcaaacg
     5581 acacaatttt tattggacct ccatacattt ttgacccgtt acttctttca atatgccttt
     5641 ccttggaatc aaaatgaaac gttggttttt tatcttttca tgatcagtaa ctttacatta
     5701 gatacacata actcgcatgt ccaggagttt ttgataagcg aaaaatacaa agacgacgta
     5761 gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag aatatttctc tttttatgtt
     5821 tttccagaag agatttcgca aaagattgcg ttgacagtga tgcaaaatca cgctcatttt
     5881 gcctatttta gaggatggat gaatattttt ggccaagaag gattaaaaca gcggttaact
     5941 gaagtagaag ataaacaatt cctggtttta tgtgaaagat tggtcagaca aacatttgct
     6001 attgttgatt ttactgaagt agacgaggaa aatgcccgct ttgaattatt tgctcgctat
     6061 gcatctattt tacggacgct gatcaagcag ttagaattac gtctgcaaat tgcttatgat
     6121 cttccttatg aaaggctaat ggcggatatc gtgaaagaaa tcatccagga acaattgctg
     6181 aatacgatca aatatgattt agtcgaatac gagaaagaaa agcagtatga tatcattttg
     6241 actagccaat tgaaagagta cccgtcacag aagtccgcta aagtttttgt attcactagc
     6301 aacgaatata attatgattt cccctatttg aaccaatttt taaaagaatg ttatttagaa
     6361 aaattgaatt taaggatgaa taaaacatga aaatcgtgat cattggtgct tcccatgaag
     6421 gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa agcagaagtt ttactgatcg
     6481 atgaagatca taaagatgga ctaggctatg tttcaaatgg aatcaacctt tatttaaaag
     6541 gaaagatacg tagtttagct gaagttgccc ataacatgcg gactcttcaa gaaagtgggg
     6601 caaaactgat caccgaatgg agagtaactg aacttgatcc agacaaacat cagctcatcc
     6661 tcgcatccaa agaaggaaaa aacgagacca taacctatga taaattaatc gtagcgacag
     6721 gttcctctcc tgtcaccttg tataaacaaa ttgaagctga aaatgtctac acctataaaa
     6781 atctagtgca atctaagcaa gtacttgccg ctttaaaaga agcaaaagaa gtagtgatct
     6841 tcggtgctgg ttacattgga ttggaattag cagatgcttt gagaaataaa ggatacatta
     6901 ttcatctagt tgattatatg ccgaatgttt tatcaagata tttcgataag gatatgatca
     6961 acagttttca aaatcagctg caaactaagc aaataaattt ttatccaaat gaattcttga
     7021 tcgattggaa aaaatcagaa gagaaagtag tatccgtaca acttctgtct caaacaataa
     7081 aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa tacgacattg ttaaaagaaa
     7141 aagtggcttt gtatgaggat gagactgtga tagtcaatga atatctgcag acaagtgatc
     7201 cggacatcta tgcgattgga gatatcgtcc cagtcagttt tgacaaaaat aaacggcatc
     7261 tctttttacc tttagttact cgagctgtac atatggcaag agcagtagcg ctaactttga
     7321 gtggtcagcc aaccgcttac gacctcagac aaaagataac agcagcagtc attatagact
     7381 actttttagg aactgtaggg ctgacagaag atgaagcgcc attcttggaa caaaataccg
     7441 gttcgtgttc aggagaattt gatctatttc cacaatacga cgaagaaaac aaaacagtaa
     7501 atgcaaagct gatctaccat ccggatacat tagaaataat tggcggacag ctgataagcc
     7561 aagaattctt attatcagat ttgaacctgc ttgcggatat cgtgaaacac aaaactacga
     7621 ttccacagct agcagttgag gattttggat ttctgtctga atacacctct cgttttcact
     7681 acctcaatga gttagctttt aaagtactag ctaagagagc aaatagcaat agagtagata
     7741 aaagactgtg agaaatatca cggtctttta ttgtgtgggg atataataaa tattaatcaa
     7801 tcgagaataa aaaattcata taatgaaata gcggaagaat tttttgagca taatattcgc
     7861 caaaaattta tcaagtgata aaattgttgt ataaaatgat tatttcagga ggaggagtta
     7921 aatgttttta gcattaaatg aaattatgca ttcaaagtta cgttatgcgt tagttgcagg
     7981 ggtgatgttt ttgatcgcct acctggtatt ttttttgact gggttagctt atggcttagc
     8041 tcaagataat cgaacagcag tcgacaagtg ggaggcagat tcgattgtgc tttcgaaaga
     8101 tgcaaattca aatctaggga tgtccatgat tactaagaag atagcagaag aagtcgaagg
     8161 tgggaaagtg gcgtatcttg cccaaacacc tggagtcgtg actagtaaag acagtacaga
     8221 agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa aatcaattca tcatgcccaa
     8281 tcttgttgaa ggaaaagctt ttgataacga tgatgaggca gtaggcgata ttagtttgaa
     8341 agaagaatat ggtctagcca taggagatac agttaagtta tctggtagtg acaagacgtt
     8401 taaactaact ggctttacgg atcatgccaa attcaatgtc tcaccagttc tctatacaac
     8461 catcaatgct taccaagaaa tccgcttcga aaaagaagat acaagtgaaa atgcacggat
     8521 taacgcaata gttgtcagag gtaaaatcag taatttacct gaagatttag aacagatcaa
     8581 aatttctaaa ttcatcaatg aattacctgg atataatgcg caagtattga cttttggatt
     8641 tatgatcgga tttttgatcg ttattgcggc tattgtgatc gggattttca tttatgtact
     8701 gacgatgcaa aagattaata tctttggtgt aatgaaagca caagggatca caggaggatt
     8761 tattgcgcgt tctgtagtgg cacaaacatt cattttatca tttgttggta ttcttcttgg
     8821 tctattagga acagtgggaa catcccttgt tctacctgat gcggttccat tccaaagcaa
     8881 ttggctgttt tttggcgtca ttagcttgtt gatgttagta gtagctgtgt taggcgcact
     8941 attttctgtt cggacaatcg taaaaatcga tccgttgaag gcaattggat aagaaggagg
     9001 aagaacatga aagcaataga atttaaagaa gtagaaaaaa gtttccaaga tggcgaccaa
     9061 gtagttaaag cattgaaaga aacaaccatg agtatcgata aaggggaatt tgttgcaatc
     9121 atcggtcctt ctggttcagg taaaagtact tttttgactt tggcaggcgg attgcagacg
     9181 cctagcagtg gctctgtatt tatcaatgat caagaattca gtaatcaaaa agaaagcgaa
     9241 cgcgttaaac tacgttttaa agaaattggc tttattttgc aagcttccaa tctggttcct
     9301 tttctgacag taaaaaagca gttattatta gtagataaga ttaaacatga acatcgtcaa
     9361 aaagaagcaa acgaattatt tgagcagctt ggcgtggata agttgatcaa caagtatcca
     9421 gaagaactat ctggtggaga acggcaaagg gtcgcaatcg ctcgtgcatt gtacaatgat
     9481 ccgtccatta ttctagcaga tgaaccaact gctagtttgg attcagaaaa ggcacgagaa
     9541 gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata aagcaacgat catggttact
     9601 cacgatacac ggctgattga caaatgcgat agaatattta tgatggaaga tggtgtattg
     9661 aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc catcgataca acaaagatac
     9721 acagcaaaaa attttgaaag aagcgactga cttgtttatg tccaagggat atctcggcac
     9781 ttcaacaaga gaaatcgcac aaaaagttgg gatcacccag cccaatctct atcattattt
     9841 cggtgataaa gaaaaactat atacggcagt tttagaaaac cacttaaaag atgttggaaa
     9901 agctttgaga gaaatcgttc aaacaagcga aacgggattt caagtaacct tgacaaaaat
     9961 ggcgcagttt ttgattgaga cacatctggt cgatttgttt ctgatgcttc acgatcttga
    10021 aagcaatctt tcaaaggaaa caagagatca tcttttcttt ctatggaaga aaaattatcg
    10081 tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg ctaagaaatg ggatttcaaa
    10141 agaaatcgca gcaaggcatt ttttcttagt gttagcacct tacatcactc ggtcgaaaag
    10201 acctgtggaa gaaccgctaa atgtggcgca attgatcgat ttatatttga atggtgtatt
    10261 aggagaagcg taaagctgtt atatagggag aaaatagtta ggtgaagaag caagatatga
    10321 cgaagatcgt tatatcttgc ttctttatat aggtagtttt tcataaatac aaatttgtta
    10381 ttctctcatg attatcaggg tatattttta tttattatgt cggtgttcca ggaagtgttt
    10441 taatttaata acgaagaaat taattgtttg tagaatgaag gaaactattt attgggtaga
    10501 tgaatcacca cgaatagtca aaaggagatt tcaggatatg ccaaaaatga aaaaaatcat
    10561 tttagcagta atgttatttt ctacggttat atcttttaca gccccttttt tgcttactta
    10621 agctgatttt aagttttggc ccttagtctg gatctacgag gtgagagtca agaatgaaaa
    10681 aaataattgg agcttttgta tttgcaatca ttatcattat tatctgtttt caaatagatg
    10741 tttttggtca aaaaaagata gttggacaaa cggtcagcag tacgaacagt acggttacaa
    10801 agacaaagga atcatttgat gatacagaac aagagacatt aatcaaaatc ggaaaaaatg
    10861 accgtacact atatacagat gactcgctaa ctaccccact tgcaactata aacggcggag
    10921 agctggcgga gtttctttct gaaacaaaaa atgcttatca gatcaagaca aatgatggct
    10981 atactggata tttagccctc gaagatggaa ccaaagtcac aaagaatata caaacaaaac
    11041 cggaagagct atctgatgca gtgattgtat tagatcctgg gcatggcgga aatgatactg
    11101 gggcattaag caatgatgaa caaacagaag aaaaagatat aacattaagt acagccataa
    11161 aagtaaaaaa ggcgctagag gatgctggag caacggttta tttgacccat actacagatg
    11221 aattagtaca gcttggagat atatgcgact acagtgaaga aaaaaaggca gatgtcttta
    11281 tcagtttaca cgcagactct acggaatatg cgaatgaagc aactggaatc acgacatatt
    11341 attactatgg acaagaggaa acattagcac agacaattgc tgactccttc actgatctgc
    11401 ctctggattc aagaggaatc tccactggga actatcaagt tcttcgagaa aatcttcaac
    11461 cttctatttt aatcgagatg ggttatatga ataatgatag tgatttaaag gaattagtaa
    11521 cagacagtta tcagcagcag attgctgcaa gtttgacaca agcattgacc acttattttc
    11581 agtaataaat aagcccagcc aaacaaaaat agctcctcat atttttcgtt tggccgggct
    11641 tattatcgca gatgcaagct tttaaacacc gtttaaggaa gaggtcgtga gcctgccgtt
    11701 caactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttga tgactattgt
    11761 gacttgtgac cgaggagttg gcatgagaac accgtttaag aaaaaggttg tgaaatcaac
    11821 gttttgttct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
    11881 ttgagctaat accgaggatc agatgattga acaccgttta ttcggaacaa gggactgtga
    11941 catgactttt gtcacagttc cattattttt ttagtagctt caaagctttt tctgtattgg
    12001 caaaatgaag tttggcatat ttttcaagca taggcatgcc gcctttctcc aatagttttg
    12061 ccgctacttg gtcagatttc gttccagctc ctgacggaag ggtgattcca gcttcagctg
    12121 atgctttgtc taactcttcc aaaaagctgg cacgactgat gatagaagca gcagcaacag
    12181 ctaaatgata ttgttctcct ttagtaacga agtacagttt ttcttgtaat tgattttttt
    12241 ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa ctgatcgatc aaaatcgctt
    12301 ctggtttttc tggctcgatt ttttttagca gcaaatggat cgcttgattg tgcaaagcga
    12361 ctttcatccg aacggcattg tattttggtt ggatctcatt gtatttttct ggagataaga
    12421 caagcagttt ataggggatg gtttttttta agatgtcagc aagctggatg atttggctat
    12481 cttttaactc ttttgaatct ctaacaccaa gtttacgtaa ttctgcaagc tgatcacgtt
    12541 tcacataagc agcacatact gttagcggac cgaaatagct gccatttccg acctcatcac
    12601 tacctaaaac ggataattga ctgatatttt ttggcaaaga agaggctgtt tttgctggct
    12661 tggcttgttt atcctgtgcc tccccccatt tatcagcttc tattgctgca ttcgtacctt
    12721 gaaacatcac ttttccggat gtataagcgg taatggtcgt cccgtctttt tttgcagcaa
    12781 agctagtata aggaatctgt ttatttaaca aataagagcg atagtatgct ttcatttgac
    12841 taagttggtc gtttgttgct ttgataatga cgttgctcat aaaattctcc tcgtttttat
    12901 acataaaact ataccgcaac tcacgacttt tcgttttcta agagattgcg agacatagca
    12961 tttttttaaa gtttatcata taatagttaa gtatgtgaaa aatatttgtg gagaacggtg
    13021 aaagaaccaa cttcatggta cactaaataa aactttcatc aaataggagg aaatgattcg
    13081 tatggcgcac gaaaaaacac gctacaaagc ggtgattgcc aaccaaacat atactattat
    13141 cggacgggaa accaagcatc atatggatat cgtaacgaag ctgatcaatg aacagttagc
    13201 agaattgaaa caattatcac cacaaatgga taatgaacaa gcagcaatct tgatggctgt
    13261 caatgcgctt tcggatcaat tgaagaaaca agaaagaata ttggagctgg aagaagaaac
    13321 agcagaatta aagaaaaaaa tgatcaaatt tacagagctt gaaaatcgtg tgaaacgcat
    13381 tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa aatggtcaag cagatcatac
    13441 cattaaaaat catgtggaag cacaacagat tttaaacgag aaacgaaaag gacaaattaa
    13501 acaaaaatct tctaattaag atccttttat caaaataaga aaaatttaaa aaagttagaa
    13561 gagaaaggaa cgtagcatgc tgagtttgct gattttattt ttattgctaa ttgcgttttt
    13621 ttctggagca cgtcgcgggt ttgctttgca aatcatattc gcgattggat atgtattgtc
    13681 atttattgct gcccagcatt tttacaaacc attagcaagt catttggaat tatacattcc
    13741 atacccagct gtcaccccaa catcaaagtt agcatttttt gatcaggtat ttgcctttca
    13801 cctagatgaa gctttttatg caggaactgc ttttctgatt attttgctaa ttggctggct
    13861 gcttacccga tttgtggggg tttttgtcca tggattgacg tatgtgccga tcttgagaca
    13921 ggtagactgg attgcaggag gcatcctgtc actaatcatg gcttacgtta ctatattttt
    13981 gattttgcag ttacttgcct ttgttccatt agatgtggtc caaaatcaat tcggcgagca
    14041 tagtttcgct cgttttatcg ttgagagaac gccatttctt accaacaaga tgtatgagct
    14101 ttgggtaacg aatattttga attaaggaaa atagtgctgg aaaatatttc gttttttgta
    14161 cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg gcacagctct tttttcatag
    14221 tgaaagataa ggtgataaaa tgaataaccg aatccttgaa acattggaat ttgaaaaagt
    14281 caaacagatg gtcagacaat ttgtagtgac tgcacaagga aaagaagaac tggcagaatt
    14341 ggtaccagtg agtgagaaac aaacgatcac aaactggctt caagaaactg aggatggatt
    14401 gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa attgaaaata tccgtcccca
    14461 tatgaaacgg attgagatcg gagcagactt gaatggagtg gagcttgcac aagtttccag
    14521 agtattgtct acgacaagcg aattgaatcg tttcattgat gatctttctg acagtgaaat
    14581 cgaatttgca cgtttgtata tgtgggcaga tcagttagtc acgatccctg ttttgagccg
    14641 ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact gatgacgctt ctcctgagtt
    14701 aaagagcata agacagaaca ttcggcgaag tgaacaagct gttcgtgaac agttagatgg
    14761 gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg atcatcacca tgcgaaatga
    14821 ccgctacgtt atcccagtca aacaagaata ccgcggtgta tttggcggtg tagtccacga
    14881 tcaaagtgct tcaggtcaga ctttatttat tgaaccaaaa caagtggtgg atttgaataa
    14941 tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt caacgtattt taagtgaact
    15001 ttctgctgaa cttgttcccc atcgtcaaga aatcatccac aatacttatg tgattggtaa
    15061 aatggatctg atgaatgcaa aagcacgatt tggtaaagaa gtaaaagcca tcgtgccggg
    15121 aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga catccactga tcgatcagga
    15181 aaaagtagtt tctaatgaca tcacaatcgg aaaagactat caagccatcg tcattacagg
    15241 acctaacaca ggtgggaaaa cgatcacgtt gaagacttta ggtctgttac aactgatggg
    15301 gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa atcggtattt ttgaggaagt
    15361 atttgcggat ataggagacg aacagtcgat tgagcaaagt ctatcgacct tttcttctca
    15421 tatgacgaac actgtagata ttttatcaaa agtcaatgaa aaaagtctgg tattatttga
    15481 cgagctcggt gcaggaacag atccgcaaga aggtgctgct ctagcaatcg caatcttaga
    15541 tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg cattatcctg aattgaaagt
    15601 ttatggatat aatcgggcaa atacgattaa tgccagcatg gagtttgatg tggatacatt
    15661 aagtccaacg tatcgtttgc tgattggcgt tcctggacga agcaatgcgt ttgaaatctc
    15721 tagccgatta ggtcttgata cagaagttat tgatgaagcg aagcagttga tgaatgatga
    15781 gagccaagat ttaaacgaaa tgatcacaga tttagagaat cgccggaaaa tggcagagac
    15841 cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa gaacttcacg atgatttgaa
    15901 agaagcttac agctatttct ttgaagaacg tgaaaaagaa atggaaaaag caaagaaaaa
    15961 agcaaatgaa gtcgtgtcag aggcggaaga aaaagcagaa aaaatcattg ctgatattcg
    16021 taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag catcagttga tcgatgcaaa
    16081 gacccaatta gcaaatctac atcaagaaga aactttgaag aaaaataaag tattgaagaa
    16141 ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg ttggttacga cttacggcca
    16201 aagagggacg cttttacgta aaaacgggaa tcagtggcaa gtagaaatcg gtatattaaa
    16261 aatgaatgtg tccgaagacg agctgacgcc tgttgctcct caaaaagagc cgacacaacg
    16321 agttatccat gctgtacgat ctgaatctag tagtcacgtg ccgaatcaat tggaccttcg
    16381 aggaaaacga tacgaagaag cgctaagtga ggtagatcaa tatttggatt ctgccattct
    16441 agctggttat cctcaagtga ctattgtcca cggaaaagga acaggcgcac ttcgcaaggg
    16501 aattactgat tatcttaaaa accatcggag cgtcaaaagc tttgagtttg caccggcaaa
    16561 ccaaggcgga aatggtgcga caattgttaa atttaagtaa agcttactaa aggtaagcag
    16621 atggttcgct atccaagtat agaaatgcta tactagtcat agattttaaa aatggaggtc
    16681 tttgatatgt cacaagtaat tacagataag acatttaatg aagaaacaga taaaggactt
    16741 gtattgattg atttttgggc aacttggtgt ggtccatgtc gtatgcaagc accgattttg
    16801 gatcagttgg aacaagaata tgatgaagaa gaattcagaa ttgctaaaat ggatgtggat
    16861 gaaaatccag aaactcctca acaattcggt atcatgagca ttccaacatt aatgttgaaa
    16921 aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt caaaagaaca actacgtcaa
    16981 atgatcgatc aatatttata aaaacttaca gcaaaaataa gctgagggtg ggactgaatc
    17041 cagttccgtc ctcggttttt ttgcggttga gttaaggtta ttcaaattaa tattttttga
    17101 gtataattac gttgtcaggc tggattgttt ttttgacaga catgctataa taactttaat
    17161 tgaatatgaa ggggagtacc agatgaacga acgaatcaaa aacaaattag ctttacttcc
    17221 tgatcagccc ggctgttact tgatgaaaga taaaaatgga acgatcattt atgtcggcaa
    17281 agccaaaata ttaaaaaatc gtgtccgctc atattttact ggtagccata atacgaaaac
    17341 ggaacggctt gttagtgaaa tcgaagactt tgaatacatc gttacagaat cgaatatcga
    17401 agcattgctc ttagagatca atctgataaa gaaaaatgat cctaaataca acattatgtt
    17461 aaaggatgat aaaacctatc ctttccttaa gataacgaat gaaaaatatc cccgtttggt
    17521 gattactcgt aaggtactaa aagacaaagc actgtatttt ggaccatatc ccgatgtcgg
    17581 tgcggcgaat gagacaaaga agatcctcga tcgattattt cctctgagaa aatgcaaacc
    17641 tagtcagacg aaggagcctt gtctttacta tcatttggga caatgcctgt gtccttatta
    17701 tttcgacgta gatccgaaag tctatacgaa aatcgttgat gaagtgaaaa actttttaaa
    17761 cggaggacac gaaaaaatcg agtcagagat ccatcagaaa atggaaaaag ctgctgaaaa
    17821 catggagttt gaaaaagctg cagaataccg agaccagatc cgtgccattg agacaatcat
    17881 gactcgccaa aaaatgacaa atactgattt actggatcga gatgtatttg gttatgcgat
    17941 tgataaagga tggatgtgtg tccaagtgtt tttcgtaaga caaggaaagc taattgaacg
    18001 agatgtttca atgtttcctt tctataacga agcagaagaa gatttcttga cttacctcgg
    18061 tcagttttat caggaaaatg agcatttcat tcctaaagaa gtcttgattc ctgatacgat
    18121 cgacaagccc agtgtagaag ctttgttagc tacgaaagtg ctgcaaccaa aacgaggaga
    18181 aaagaaaaaa ttagttgaat tggcgagtaa gaatgcctca gttgctttga atgaacggtt
    18241 tgatctgatt gccagaaaac aggaacggac gatcggagca gttgaacgtt taggaaacgc
    18301 gatgaatatc ccgaccccaa tacgtatcga gtcttttgat aactctaata tcatggggac
    18361 gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa ccggcaagaa aagaatatcg
    18421 gaaatataaa ataaaaacag tcaacggacc ggatgattat gcatcaatga gagaagtcat
    18481 ctatcgcaga tattcgcgag tgattcgcga agggttgcct ttacctgatt tgatcttgat
    18541 cgatggagga aaaggacaag tagatgtggc aaaagatgtc ttagaaaatc aattaggaat
    18601 cgatattcct attgctggca tggtaaaaaa cgataaacat aaaacaaatg agttgctatt
    18661 cggtacagat ttggctgtag taccgcttga gcgaaattca ccagagtttt tcttactcca
    18721 gcgaatccaa gatgaagtcc atcgattcgc aatcactttc catcggcagc tacgcagtaa
    18781 aaacagtttt gcttcccgat tagatgaaat tgaagggtta ggaccaaaac gaaaaaaaat
    18841 gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca gccacgacag aagaattaca
    18901 agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt gataaattac atcaggaatg
    18961 aggaaagggc actttacaaa gaaagtaaag tgtcctatca ttttatgtac aacaaaaaga
    19021 gcgtttacgt tttgttggta aataataaat cgcttacgtc tggtgtctaa ggatatatgt
    19081 gatttcttga agaaggagtg aagaaatgtt agaagtcaag gatcttgtta aaacttttgg
    19141 gaactacaca gctgtcgatc atgtctcttt ccaaatcccc gatggaaaaa tcatggggct
    19201 aattggccaa aacggggctg ggaagacaac gacttttcgt ttgatcttgg attttttgac
    19261 acaagaccaa ggagaagtgt tatggaatgg ccatcatctt ggggagaaag attatgatgt
    19321 catcggttat cttccggagg aacggggact ttatccgaaa gtaacgattc aagaccaact
    19381 gctttatttt gcagaactta gagggaaatc aagaaaagag attgaaccaa aaatcgactt
    19441 ttggatggaa aaatttcagg taaaagggaa aaagacggat aaagtaaaat ctttgtctaa
    19501 aggaaatcag caaaaagtcc agctgatcgc cacgttaatc catgaaccta aattgatcat
    19561 tttggatgag ccattcagcg ggttagatcc ggtaaatgct gaattgctaa aagatggaat
    19621 attggaattg aaaaaacatg gttcatgtgt cattttttct agccacaata tggataatgt
    19681 agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga aaaatggttt tgaatggaaa
    19741 agtacatgag attcgtgaat catttggccg tacgaagtta ttcttggaat ctggtttaag
    19801 ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg atccttcatg aagacaatac
    19861 gttagagatt accttagaag atcctgaagc tgggaaagaa atctttactc gtgcgactca
    19921 gttcggctac attcctatgt ttaaccagca accgccaact ttggaagaga ttttcaaatt
    19981 gaaagcaggt gaaatcgatg cgtaagtttt ggattattac caaagatgtc tatttaaaaa
    20041 atgttaagtc gatttctttc ttgatcatga tattagtccc ttttatcatg ctgggtatca
    20101 tttatgtagc aggaaacttt gctcaacaaa atagtgatgt caataaaatc ggcgtattgg
    20161 ctgataatga gcaaatcagt cagcaaatga gccaagtcaa aacagatgat tttgagttca
    20221 aagcagttga ttctgaagaa aaagcacaaa aagagctttc ggaagaaaaa atcgatgcgt
    20281 atttagtagt ctcaactgag aatgataaga taaatgggat gctttatagc gaaaattcat
    20341 taggacaaag tacacaacta ctgatacaac aacaactgac tggcttgcaa tcgatgcttc
    20401 gagcgagtca attaggcatt tcaccagaag aagtagctag cttgagccaa caagcaagtt
    20461 tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat gacagtcgga gaagataact
    20521 cgacagtcca atacgtcgta agttatgtag caacgattgt cttgttcatt attattttga
    20581 cgtatgcgca gatcattgca caagagatcg cttctgaaaa aggcacgcgg atcatggaag
    20641 tgatcctgtc cagtacgact gcacaaaagc atttttacgg aaaattaaca ggtgttttac
    20701 ttgttgcatt aacgcaaatg gcattatacg gtattatttt tggtatagga tacagtcaat
    20761 tcaaagatat ggaaatggtc aaaaatatat tagacggtat ctcattggat aacttgattg
    20821 gaccattctt gtggtattct cttctattca tgatctttgg cattttgatt ttttctgttt
    20881 tagcagcttt atgtggctct ttggtcaaca aagcagaaga tacagcaaaa gcaattttgc
    20941 cagtgactta tctatctcta ggcggataca tgcttggatt gatactagga gcttcagatc
    21001 caaacaatat cgttattcgg atcacttcgt atattccatt cttatcttca tatattatgc
    21061 cgattcgctt agcaaatgag acagtcggta tgggtggagc attgatttcc ttgttggtac
    21121 ttgtagccgt aacattctta ttgatgatct tgtctgcaaa catgtacaaa tcgaatgtac
    21181 tcgtttatag cgaaggcgga ttatggagtt cattgaaaca atcgatttca attatgagga
    21241 atgaacgaaa aaaagcataa aacaatccta aaatacagaa ggaagctgga catctattgt
    21301 tcccagcttc tttttgtata gacctatttt atttatttta ctatattgga tatatattca
    21361 ttgaaaatag tgtataacaa ctttataata ttgagtatac caatttttgg aggcgtttta
    21421 atggaccgtt atttagattt actttcagaa aaattcccct caacggagga tgtcatcacg
    21481 gaaatcatca atctcgaagc aattttgcag cttccaaaag gaacagagtt atttttgagt
    21541 gatatacatg gcgaatttcc tgcttttgat catattctgc gtatcggttc aggaaatctt
    21601 aaagaaaaga tcaaagacct tttttctgaa caaatgacgg aaacggatat cagccaattc
    21661 actattttta cggcttatcc agaatatgca ttaacgactg attggtacaa agaacaagat
    21721 aaatcgcagc tgatccatca tttgatcgat cttttacggt ttacgacagt caaatatacc
    21781 cggtcaaaag ttcgaaaagg attgccgaaa gaatatagct atatcattga ggaactcttg
    21841 tatatcgatg accgtatcaa tggaaaaaaa gactatgtaa aaaaaatcat tgaacaactt
    21901 gttttgatga aggaagaaga aaaattttta acgaaacttg cacagacgat ccaaaaatct
    21961 gtcatcgatc atttgcatat cgttggagat atttttgacc gaggaactca agcagccaaa
    22021 gtgatggatc gaatcatgga tttttcttct gtagatatcc aatggggaaa tcatgatatt
    22081 ttgtggattg gtgcgtattg cggctctgag gcatgtttac tgacactttt acggattgca
    22141 gcacgctata attatttatt tgaattagag aaagaatatg ggttgaattt acgtccattg
    22201 ttttcttttg ctcatcaaac ttatcagaag aatccagtat ttgcgcctaa aaacagtaaa
    22261 aatctatccg aaagagagca agaagaatta gaaaaaatcc atcaggcact ttctatcctt
    22321 caattcaaat cagaacatca gttattggat cgtcgacctg aatttaaaat ggacgatcgt
    22381 aaattgctgg aaaaaatcga ctatgaagca tcaacgattg atttagaagg tcagctctat
    22441 gagttgaaag gaacttgttt tcaaacgatc cagccagatg atccaaaaaa attcttggaa
    22501 gaggaacaaa aagtgattga tagtttgatg tattcattcc agcactcttt gagaatgcaa
    22561 aaacatatga cgtttttgat cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgtg
    22621 ctgtttcatg gctgtatacc tgtagacgag aagggacagc tcttagcatt tgaattagac
    22681 caatcttatc gcggaaaaga actgctgggc ttttttgaaa aacagataac tgaaagcgca
    22741 aaagatctga ctgcaaaaga agatttagca accgatttga tttggtatgc gtggagtggg
    22801 ccattttcgc ctttattcgg caagagtaaa atggcgactt ttgaacgtta ttttctcaca
    22861 gagcctcata cgcacgtcga gaaaagcaat ccttattatc acttgcgaga tgaagagtgg
    22921 ttcagtgaaa aaattttagc agaattcgac acaaaagaag aagggtcagc aatcattaat
    22981 gggcacacgc cagtaaaagt aaaaaaaggg gagtcaccga tcaaagcaaa tggaaaagta
    23041 tttgtcattg acggaggttt atccaaagct tatcaaaaaa cgacaggaat tgccggttat
    23101 tcactattat gcaattctta tggtttccaa atcgtgacac attatccgtt tttgcctatt
    23161 gaacaacaat ttgcaaaaaa atccgaccaa acaaatgtga aaaaagtaat cgaacagcaa
    23221 ctaccacgca aactcaatcg tgatacaaca aaaggtatgg aactgcagca gcagattacc
    23281 cagctcaaaa ggctgttgga ctatcgaaag gacgattgag aagaattcca gtgaacattg
    23341 ttccatagac tggagactga gacaaaagtc gtgtcgcagt ctctttttcc ggataaacgg
    23401 tgttcttctg ccaactcctc ggtcacaagt cacactattc aacaaacatg aaaagctgtt
    23461 ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct cttggtataa
    23521 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtgaaaagc
    23581 cattttcaca aaatttttct taatactcga aactaaacag ctttttcaca accccctcag
    23641 aaagtctatt aaaattcatt cacaaagaaa tcgaataagc gctgttcaga tggatgtaca
    23701 cggtgggtaa gttccggatg ccattgaaca ccgaggattt tatgcatatc atctttgctt
    23761 tgaaccgctt caacgagtcc atcgttagaa aaagcgatgg cttttaataa aggtgaaaga
    23821 tctttgactg cctgatgatg gtaagaattc acaaaatatg gatcggggag aaggttgctt
    23881 aacaaactgt ctgaaacaat cttgacttcg tgagtcgtaa attgcggggc agtgggctgt
    23941 tgttcatgtt ttactgacca ctttggatat aatgaaagat cttgataaag tgtaccttcc
    24001 aaaacgacat tcaatagttg gagtccccgg cagactgcaa aaattggttt tctttgtttc
    24061 aatgcttcca agatcagtgc ctgttcaaat tggtctcgtt gaatatttgt ttccgttagc
    24121 tttggatgag gatcttccat gtaaagttgt ggactgacat cttgaccacc tgcaagaata
    24181 agtttgtcga tttgtccaat cagttgagga gctgaatctg gagacatgac tggcagaata
    24241 agaggcaatc cttgagcatc taaaactgca tccacgaatc cttgcggtgt atagctcact
    24301 tgatttcctt ggaaagtatc tgttgctcga atcagttgat ttccggcaat gccgatgatt
    24361 ggtttcattt gtattccccc taaagtagac ttattgtaac aaggatttaa aaaaaattaa
    24421 actatgtaag acttgcccct atacttatct gttcttgctg tgttactatc tgtaaagaga
    24481 aatttcgtgt gaaattcatg acgatagttg aaaatattgc ttaatttgtt ataataaaaa
    24541 gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc aaggggtgag
    24601 aaggacattt tattgaaggg gcctgcctat actaagttat ctttagaggt acatactttc
    24661 aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag caagtgttag
    24721 aagctttgga acaaatccga aaagcagaag aagaaaatga aaagcgtgct caaacactgc
    24781 ggcagcagct gaagatctat gcaacagaaa aagagcagga attggaaacg atccaaacaa
    24841 actatcgaca acaactaaaa cagttggtgg aagaaaaaga acaatttgaa atacaagaat
    24901 tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca actttgaaag
    24961 atcaatatca aaagcatcag agtcaagcga tcggtgcgat cattgaaagg gtgaaagaaa
    25021 cttatggccg tcactaagat ggaaaaagtg accctcattt cagataaaag aaatcaggag
    25081 aggattttac aagcagtaca aggaatccaa aacgttgaaa tccgggatct gtatcaaaat
    25141 agaacaaaca accagtgggt ggagaagtat tttccagata ccacgattat tgataaagaa
    25201 gcaaaattaa gcgctttagg taatcggttg aaagacatcc aagaagctat ccaattcata
    25261 caacaccacg gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga actttcctta
    25321 caagatttgg aagccaatta ttccgaagaa aatttcataa ataaattgaa agaagtcctt
    25381 gatttaaaag aaaagtggga acaactaact gaagaacgtg atcgtttaga agaagaggaa
    25441 gaatggcttc ttcattggca acagctggat attgctccaa gtatgtatgc aagtaagacg
    25501 accatttttg agatggggac tgtgagtgca gcaaattttg aaagtttcaa ggaagaactg
    25561 agtaagcttg atgacgttta tatggaagaa atagattata cgccaaaaca agtttatctt
    25621 tcgtatgttg ctttaaaaaa atcagttgct gttatagaag aaattgctgg gaaatacggg
    25681 ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag aaaaactgca agagaccaag
    25741 cagcaattat ccattgttca cgagcagcaa aagaagataa cgacagcgtt aggtcagtgc
    25801 agtggctata tccaagaatt tgaatgggta gaagaagtca cacttgccgt tgcagaacga
    25861 gaaaaaataa aagaacggtt tatccatgcg acttatctga tcgttttgca aggttgggta
    25921 gatgcagaag aaaaacagaa tttgctgcat gtgttaaacg aaaccgtatc agaagaagat
    25981 atatacgtca gtttcgaaac accaaactca gaagagattc agcaggaagt ccctactaaa
    26041 ttgaaaaacc atccgcttgt cgaacctttt gaattgctga ctgaaatgta cagtctgcca
    26101 aaatatgaag aagtagatcc aacaccttgg atgacaccat tttatcttgt cttcttcggg
    26161 atgatggtcg cagatgttgg ttatggtttg ctgatgctca ttgggacaat actagctcaa
    26221 aaactacttg ttctaccacg cgggatgaaa cgtttcgtaa aattctttga gattctctcg
    26281 ataccatcga tcatctgggg attgatctac agttcatttt tcggacagtc attaccaaaa
    26341 gaactgtttg gcatccgctt gcctttccca atcctatcta cgacggatga cgtcaatacg
    26401 attttgattt tatccgtgat tttcggtctg atacagattt tggttggatt atttgtggca
    26461 gcaaaggaaa atatcaggag aaaagattac ttgagtgctg tcagtgatgg atttgcctgg
    26521 caagggatcc taattgggat cgttattgcg ttggttggtg ccatgttgat aaaaaataca
    26581 gcatttgttt accttggcgc tgggctatcg atcattgctg ccttatgtat cttgatcgtt
    26641 ccgattatcc aaacgccgtc taaagtcaaa ggtgctgcca aaggtgcgta taatctttac
    26701 ggactgaccg gctatatcgg tgatttagtc agttatacac gtttgatggc attgggtatt
    26761 tcaggcggaa gtattggagc ggcattcaat atgctagttg ctttcatgcc gccagctgct
    26821 cgattcagtg tagggatcct tttgatcatc gctttacacg cactgaatat gtttttgaca
    26881 ctgctaagtg cctatgtcca tggcgcacga ttgcagtatg tcgaattttt tgggaagttt
    26941 tatacaggag gcggacgtgc ctttgatccg ctgaaaacgg cggaaaaata cgtcaacatc
    27001 aatcacaaga aaaaaaacaa taaataattg gaggattttt atataatgat ggattactta
    27061 attagtcaaa atggtggaat ggtatttgcg gtattagcga tggcaacagc gacgattttt
    27121 tcagggatcg gttcagctaa aggtgttgga atgacaggtg aggcagcagc agcattgaca
    27181 acaagtcaac ccgaaaaatt cgggcaggct ctgattctgc aattgcttcc aggcacgcaa
    27241 ggtttgtatg gattcgttat tgcctttttg atttttatca acttaggaaa tgacatgtca
    27301 gttgtacaag gactgaactt tctaggtgct tctctgccga ttgcttttac aggcttgttt
    27361 tcaggtattg cccaaggaaa agtcgcagcg gcaggtattc aaattttagc gaaaaaacct
    27421 gaacatgcta caaaaggaat catttttgct gcgatggttg aaacatacgc gattttaggt
    27481 ttcgttattt ccttcctatt agtgttgaat gcctaagcat aaaggaggaa aagcagatga
    27541 acgccattga aaaaatcatc tctcagatga acgaagcagc agagcaggaa agagcggcgt
    27601 tagaacaaga agagcgaatg aagatcgatc aaaactttga acaaaaacgt acgcaagttg
    27661 aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt actcgaaaaa aaataccgtc
    27721 agctaaggaa tcgccagcaa gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat
    27781 ttttaagacg attgtttgca gatgcggtaa ctgaaatgga aaattgggat gaatctgaac
    27841 aaatccagtt tatcaaaaat gcattgtatt cattaccttt gacaggaaaa gtagcattca
    27901 ttgctggaga aaaatcagca gcctatcttt ctcagacgtt gttggatgaa tggaacaatg
    27961 aacttccctt tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg ggttttctga
    28021 taaacgatca aggagttcag tataattttc ttttcagcag ccttgtacaa gatatccaag
    28081 gaacgatgag ttttgagatt gccaatcaat tattcgaata aggagggatc agtgtatgga
    28141 ataccacgaa ctaaatccct tgattcgcgg aagagagctt gaacttttgt ccaatgagac
    28201 atttgaacgg atgattcaaa caaattctgt cgaagcactt ggtgaaatat tgaaatccac
    28261 gatttacagt ccatatattt atgacggata tgaatatgac ttcgaacaga atcttttaaa
    28321 ggagcagagc cgtctatttc gctggttaaa ggaatcagca ccggagcctg aaatcgtttg
    28381 gatctatacg atgcgttata ctttccataa tctgaaggtt ttgacaaaag ctgagatgac
    28441 aaatcaaaat ctggaccatt tgtatatcga cgatggattt tattcattag aaaccttgaa
    28501 ggatgccatt catacgcaag tttcaaatga acttcctgaa agcattatgg acagtatacg
    28561 agaagtacgt gattattttg aagagtctac cattcttcag ggaattgacg tgatttatga
    28621 tcgacagttt ttgacagagc agcgacggct tgccgataag ctaggatatc aagaattgct
    28681 gacagaagtc attgctttta ttgacttgac caatatcaca acgatggctc gaggaatatt
    28741 gcagaaaaga acatatgggt tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa
    28801 agaagtattt ttgaactttg tagataaaga tatggaagat tacacgaatt ttctattaga
    28861 gacagattat gctgattttc tgcgccctgt catccgtgag ggaaatattg atctcgtaaa
    28921 gcttgaacag ttgaaagatg attatctaag ttcattgtat caagaagcac aaacccaggc
    28981 tttcggacca ctgccactac tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct
    29041 tcgtttactg gttatcggca aaagaagcca tttcgaatca gaaacaatca gaaagcgggt
    29101 gagacaagtc tatgacgcat aagatcggtg tagttggcga caaagattct gttttgccat
    29161 tcaaattgtt tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa
    29221 ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt cacagagcaa tgtgcagcat
    29281 tgagcccaga aacgatcgaa cgttacaagg aacaaatgat acctgctatc gtattgattc
    29341 ccaatcatca aggatcatta gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag
    29401 cagtaggtca aaatatctta tagtatgaaa aggagataga aattttgcaa attggaaaaa
    29461 tcataaaagt ctccggacct cttgtaatgg ctgagaacat gtctgatgcc agtatccaag
    29521 acatgtgtct agtgggagat ctaggagtta tcggagaaat tatcgagatg cgtggcgatg
    29581 tggcgtctat tcaagtatat gaagagactt ctggaattgg tccaggcgaa ccggttcgct
    29641 ctactggtga agctttatct gttgaattag gaccagggat catttcacaa atgtttgacg
    29701 gaatccaacg gccattagat acctttatgg aaatcacaca aagtaatttc ttagggcgcg
    29761 gagttcaatt gcccgcattg gaccatgaga aaaaatggtg gttcgaacca acagtagaag
    29821 ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca aaagttataa
    29881 aacataaaat catggtgcca aatgggataa aaggaacgat caaaacaatc gaaaaaggtt
    29941 cattcactat tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga
    30001 cgatgcttca aaaatggcca gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg
    30061 atgcacccat gatcactgga cagcgagtga ttgatacatt cttcccagtg acaaaaggag
    30121 gagcagcagc tgtacctggt ccttttggtg cagggaaaac agtcgttcag catcaaattg
    30181 caaaatgggc cgatgtggac atggttgtct atgtgggatg tggcgagcgt ggaaatgaga
    30241 tgacggatgt tttaaatgaa ttccctgaat tgattgaccc taatacagga gaatcgttga
    30301 tggaacgtac ggtattgatt gctaatacat caaacatgcc agtagcagct cgagaagctt
    30361 ctatctatac gggaatcaca attgcggaat atttccgtga catgggttat gacgttgcta
    30421 tcatggcaga ctccacttca cgatgggcag aagcacttcg tgagatgagc ggacgtttgg
    30481 aagaaatgcc tggtgatgaa ggttatccag cttatcttgg ttcacggctt gcggaatact
    30541 atgaacgatc tggaagagtc attgcattag gatcagaagg tcgcgaagga agtataaccg
    30601 caatcagtgc tgtatccccg tctggagggg atatttcgga accagtcaca caaaatacat
    30661 tgcgggtcgt aaaagtattc tggggtcttg attccagttt agcccaaaaa agacattttc
    30721 cttcgatcga ttggatacaa agctattctc tttattcaac agaagtaggg aagtatatgg
    30781 atcatatcct tcaagctgac tgggcatcca tggtcaccga gggcatgcgt attttgcagg
    30841 aagaacagca gctgaatgaa atcgtgcgtt tggtagggat tgattccctt tcagataatg
    30901 accgtctgac attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt
    30961 ttgatgatgt cgatacattc acttcaagag agaaacaatt caaaatgttg aagatgatct
    31021 tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta agtgagatca
    31081 tggacggtac cgtatcgatt cgtgaaagga tcggacgaag caaatatatc ccggaaacag
    31141 aattagaaaa actagatcaa attattggtg aaatcaagca aacgatgcaa acgatcgttt
    31201 cagaaggagg catgagcaat gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca
    31261 ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga agtccgtatg
    31321 caaaatggcg agattcgtcg tggacaagta ttggaagtcc aagaagataa agcaatggtc
    31381 caaatattcg aaggaacaag tggtatcaat ttacgtgact cttctgtacg atttttaggc
    31441 catcctttag aactaggtgt atcggaagac atgatcggtc gagtattcga tggtttggga
    31501 cgaccgaaag ataacggacc agagatcctg cctgaaaagc atttggatat taacggagaa
    31561 gtaatcaatc cagtagctcg ggattatcca gatgagttta tccaaacagg tatctctgca
    31621 atcgatcatt tgaatacatt agttcgtggg caaaaattac cagtattttc tggctctggt
    31681 cttcctcata aagaattagc tgctcagatc gcgcgacaag caacggtact cgattcatca
    31741 gatgatttcg ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc tgaatttttc
    31801 atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat gaatttagca
    31861 aacgaccctg caatcgaacg tatcgctact cctcgtatgg ctttgactgc tgcagaatat
    31921 cttgcttatg aaaagggaat gcatgttttg gttatcatga cagacatgac caactatgca
    31981 gaggcattgc gagagatttc tgcagcacgg cgagaagttc caggaagaag aggatatcct
    32041 gggtatcttt acacgaactt agctacttta ttcgaacgag caggaagaat ccgcggacta
    32101 aaaggatcgg ttactcaaat tccgattcta acaatgccag aagacgataa aactcatcca
    32161 atccctgact tgactggtta tattacagaa ggacagatca tcttgactag agagctttac
    32221 aagagtggga ttcagccacc aattgatgta ctcccgtctc tttcgcggtt aaaagataag
    32281 ggaactggac ccggaaagac aagagaagac catgcagcta cgatgaatca gctttttgca
    32341 gcctatgcac aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga atctgcttta
    32401 tctgatgtag acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat
    32461 caaggattct acacgaaccg ttcgattact gaaacacttg atctagggtg ggaactgcta
    32521 tcgatgttgc caagaaccga attaaaacga atcaaagacg acatgctgga caaatatctg
    32581 ccagaaggga agtgagttct gatggcacga ttgaatgtga atccgacccg catggaattg
    32641 actcgattga aaaaacagtt gacaactgcc acaagagggc ataaattatt aaaagataag
    32701 caagatgaac tgatgcgtca attcatcctg ttgattcgaa agaacaacca gctgcgtcaa
    32761 gaaatggaag agtctatgac agaagcaatg tctgattttg tattggcaaa tgcttcgatc
    32821 acggaacctt tcatcgaaga attatttgtt ttgccggcgg aaaacgttga tttgtctatc
    32881 gtagacaaaa atatcatgag tgtaaaagta cctgtgatga attttgaata tgatgaacag
    32941 ctgacagaca cacctttgga atacgggtat ttgaattcca gtgccgaact ggatcggtca
    33001 atcgatcggt ttactaccct gcttccaaaa cttttggaac tgacagaaat cgaaaaaact
    33061 tgtcaactga tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc attagaatat
    33121 atgacgatcc ctcaattaga agaaacgatc tattatattc gtatgaagct ggaagaaaac
    33181 gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg
    33241 gtatcttttt ttaaaagagg aaaacgatga ttaaacaaag caaacaaata aagaaacgtt
    33301 tgccggctgt tcagcttctc gcattaggat tttttctcct gattttagtt ggcggcggcc
    33361 tacttaccct accatttttt agtaatagtg ggcatgggac aaattttatc gatgcgcttt
    33421 tcactgcaac ttctgctgtc tgtgtaacgg gattgacaac attgaataca gcagagcatt
    33481 ggaacagtgc tggtcaattt ttgatcatgc ttttgattga gatcggcggt cttggtttta
    33541 tgatggttcc gattctatgc tttgcattga taaagaagaa agtcagtctc agcacacgta
    33601 ttatattaaa agaagcgctg aatctagaag aagtatcagg tgtgatgaag ctcatgcttt
    33661 acatcctaaa attggccttt gtgatccaat tgattggtac ggttgcttta tcttttgtat
    33721 ttgtgcctga atttggctgg ttgaaaggta tctggtacag tttgttccat gcaatctcta
    33781 gtttttgtaa tgcagggttt gatttgttag gagacagttt agccaatcac cagcaaaatg
    33841 tttatttgat catggtggta tctgctttga tcatttctgg tgggttaggc ttcatcgtat
    33901 ggcgtgattt actggaatat catcagaaaa agaagatgac gattcattca aaaatcgcat
    33961 taaccgttac aggtaccctt ttattaggcg gattcgttgt gttctttttc acagagcgca
    34021 atggggcaac gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc ttttttatga
    34081 gtgtgacacc tagaacggct ggatattatt cagttgatta cctccatatg agccatgcag
    34141 gtctgatttt aacgatgttt ttgatgtaca tcggcggaac atctgggtcg acggccggcg
    34201 gattgaaaac aactacgtta ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac
    34261 gtacgagagc ggaagcattt ggccgtacca tccgacaagg cgcagtgttg cgggcactta
    34321 ctttattttt tgttacgttg acgttgtgtg tagtcgccat catgatttta tcggtgactg
    34381 aaacgatacc taaaacttct ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg
    34441 gtacagtagg tcttacgatg ggattgacgc cagacttgac tgcaataggg aaattgatca
    34501 ttatagcgct tatgtacatt ggacgagttg gtgtattgac tgtggtcttc tctctgatga
    34561 caaaagcaca aagaacacaa tcaaaataca aatatccaga agaaagtgtc atgattggat
    34621 aatttcatct tctgacagaa acctagtgat ttagtaaaat aagaaaataa aataattttt
    34681 cttgcttctt ggatacttta agtgcaaaat gttcgtttat tttttgaggc actacccgtt
    34741 gttgaacctc gggtagtgcc tcttttttat tgaaaggaat caataagatt tgattccttt
    34801 atttatcaat aggttataat tcatattttt ttattttttc ttttacgata cttagtccag
    34861 aagctaaaat ttcaaggtta ttagaggata gggaaattcg aacgaatgca tctggttgcg
    34921 cacgtgtagt aaagcgtctg gaatgataaa gtcggatacc gttagcaaga aagtcattct
    34981 caattatctt agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa gggacacgat
    35041 tcgttggttt ttctactttg aaaagagaat caaaaagctg attggatctt tttgtaagac
    35101 ttaatttagt ttctaagatc ctatttgctt catttgaatg gaggatttga gtgatgactt
    35161 ccacatctaa tccagaagtt ttcacattta tattaaaaat tgcttgttct atcttgcttt
    35221 ggacatgtac tggaactaca agataagcaa ctcgcaatcc agaacaaata aattttgtca
    35281 ttccagctaa gtagattgtt tggttaggta ataaattttg gaaaggagga atgatcgtat
    35341 gttgatagta agttgttaaa aatgaatgga tatcatcttc aataacccat agaccttctt
    35401 tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac cctataggat
    35461 tattacaaga aggcataaga aagacaccat gaatcttctt ttttctacat tcctgaatca
    35521 atatatcagg tcgcattcct tgctcgtcaa aatcaattgg aacgatctct aaatgatata
    35581 actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt ctatcacctg
    35641 gagagaaaag tgcagaaagt gtgatagcca atccattttg aacaccggaa acaatagata
    35701 gagattctaa ggacggaatg actccttgag tttttaacca atttgctgca gtagtcaatt
    35761 gaaaatttgt gcctttggga tcttggtaat taagcaaacc aacaagattt gggttattac
    35821 tagttgattg aataaacggg acaacgaatt tattacattc ttcaaaagaa ctaaccagac
    35881 caaaatcaat gatatgttcg gttacttcat tggtagagat ggtgatggac tcaaagacat
    35941 tgggagaaat aaatgtgcca cttccaatat tcgtatacag caaacctttc tcaattccat
    36001 atttataagc cttgccaacg gtagtaaaat ttaaatctaa ataatcagct agctctcttt
    36061 gggagggtaa ctgagtgttt ttctgtaatt tattctttaa aatatcttgt tctaattgtt
    36121 gaatcaacga tcgataataa ggacgcttca atttttgttt gtctggtcgc caagttaacg
    36181 ggtaattgtc ataagaatta ataggcattt tctccactcc ttaaaattgt ataccataca
    36241 attttaccat tgattgtatt gattttagaa gttaatcttt tgtgaggagg gaattgtcta
    36301 tgaaaattat taattttgag gaagtagtag cttcttttag ttttgaagaa gcgattcaag
    36361 taatgaaaaa atgttttttt gattatgaaa gtggtaaaat cagccaaaat cctcgattgg
    36421 tggaaatttt acctgacgga aaaggcaaaa atatgtttgc gatgatgcct gcttatttgg
    36481 gggagaacag atattttggc acaaaagtca ttaccgcttt tccggataat catcagtatc
    36541 atttaccttc tcatttagga gagattttct tgtttgatgc tcaaaacgga ctacccaaag
    36601 cattgataaa cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct gcgctggcaa
    36661 cagattactt agcaaatccg catgcagaaa cacttgcttt aattggagct ggtcaacaag
    36721 cattgtcgca tttgactgcg atttctacca ttcgtcccat cactacagtg tttgtttacg
    36781 atattttaga agaaagacga aatactttta tcgctaaagc taaaaaacag tatcctaaaa
    36841 taacgtttat aaatgcaata tcagtagaag atgcagtacg tccagcggaa atcatttgta
    36901 cgttgacctc tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa gacgcacata
    36961 tcaatgctgt gggcacattt actcctgata caagagagat aagtacatcg ttgatggaaa
    37021 cttctcaaat ctatgtagat gattactcag ctgccttaac agaaagcggg gacctgctta
    37081 tagccatggc agaaggagat ttagaggaat cttgtattgt tggtacatta ggcgaattag
    37141 tttctgagaa aaaaacattt tataaaggag aatcgaggcc gacgattttc gacgcagttg
    37201 gtctagctat tgaagatctt tgttgtgcag aatatattta taacaaatta gagaaagggg
    37261 ctttaaaatg aacttagatt attacgtagt tgatgcattt gcagatgagg tgttcaaagg
    37321 aaatccagca gcggtttatg tgctagaaga atggctgcca gaaggtacga tgcaaaaaat
    37381 tgccattgaa aacaatttat ctgaaacagc atttacagtg aagaaaaacc aagaatttga
    37441 gcttagatgg tttacgccag atcgagaaat cgatctttgt ggtcacgcga cattagcaac
    37501 agcatttgtt ttatttaact attacaaaat acctgatgaa acgattaaat tttctactca
    37561 aagcggtaat ctatttgtga caagaattaa agattattat tacatggatt ttcctagtat
    37621 tatgccgaaa aaagtgccga tattgtctga atatgaagaa gcgattgggg ctaagattaa
    37681 agaagcctac ttagcacgtg atttattttt cgtattagaa gatgaaaaaa cggtggctaa
    37741 attgcagcca gattttacag ctatcaaaaa ttttgaatta ggaataggag tcatcgtgac
    37801 agcagaaagt gtgcaaaaag actttgtttc tagaaccttc tttccaaaat taacaatcaa
    37861 tgaagatcca gtatgtggat cagcacattc taatcttatt ccttattggg cagaaagaat
    37921 gaataaaaca aaattgtcag cttatcaaat gtcatcaagg ggcggggatc tagaatgtga
    37981 attaaaagaa gatcgtgtga taataggagg aaaagcagtt ttgttttcta aaggtacctc
    38041 gtttatttga tttctttcaa atgaattaaa aataaattgg tggagagtgg caaccttata
    38101 gctacaatgg ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa gtttgcgtct
    38161 cattttaatt attggtgttt gttttttatt caatcaagag taaaatgccc catactaact
    38221 atcctcggat gacttcgatt ttataaccat cagggtcaat gacaaagtag taagaaggcg
    38281 cagtgccagg taatcctttt aagtcagtaa tctcaagacc agcagcttta tgttgctcgt
    38341 gtaatttttc tagatcatca gctgctatag cgatatgacc atagccgtct cctagatcat
    38401 acgcaccatg atcatagttg taagtcaact ctaattcata gtcatcccca ggaagtgtca
    38461 aataaacaag tgtaaatttg ttttctggaa aatcacggcg gcggctttct tcaaaaccaa
    38521 atgccttttg ataaaattcg attgatgctt caagatcttt cactcgaaca catgtgtgag
    38581 ccattttcat agattcatct ctccttttcc ttttactcaa tcataacaaa aagaagtaag
    38641 gaaatagttg atttgtgctt gttcatgaga aatttctcat gtaaaatgga tagaaaataa
    38701 aactgttaag gtggaaacta ttcatggata aaccaatttt tggtgaaaaa aagcaatttg
    38761 ctgattatca aacccgttat gcagcatata ttgtcataga aagagatcag cagattgcag
    38821 taatcgaggc tccaaatggt gctttttttc ttcctggcgg ggaaattgaa ggagaagaga
    38881 cgaaagaaga agcgattgtc cgtgagctga tagaagaaat gggaattgtg gcagaaattt
    38941 cttcttatct gggagaagct gatgagtatt tttactcgaa ttatcgtgca acttattatt
    39001 ataatccagg atatttcttt gttgctgatt cttggaagcg tataggagaa ccaactgaaa
    39061 agaccaataa aatttggtgg gtgagccctc aagaagcgct tgtgaaattg aaacgtggta
    39121 gccaccgatg ggcagtcgag agatggatcg aaaaaaataa aaactgaaag aaagcgagta
    39181 gatcagcttt ctctcagttt ttttatttat tacagtacat attttttgat tgcttcagct
    39241 acaccgtgct cgtcgtttga tgaagtgatg acatttgctg cttctttgac aagagaaacg
    39301 gcatttccca ttgctactcc tagaccagca tattcaatca tggataagtc gttctcgtta
    39361 tctccgatag ccatgatttc atcttgatca ataccaaggt gctgtgcgag attcgcaact
    39421 gccgcacctt tacttgcttc tttatttagt acttcgaagt aatagggcgc acttttgacc
    39481 gttgtgtatt tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc taaaatttct
    39541 ggttcgtcga tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc ttcaggtgtt
    39601 cgatatttta gcggcatctt gactaaactt gcttcgtgga tcgtataagc gctgatatct
    39661 cgattaggcg tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc gacacggcgt
    39721 gccattactt cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc gatgatttta
    39781 cctgatttcg tttgttgtac gagtgccccg ttataagtaa taacgtaatc attatcttga
    39841 aaaaggttca actcgtttaa ctgttcctgg acacctggta agggacggcc agtgcataaa
    39901 acgatcgaaa caccttttgc aacagcagcg gcaatcgctt cttttacttc ttttgttact
    39961 tctcttttct catttaataa agtaccatcg atgtcgatag cgactagttt gatagacata
    40021 atttcctcca atatatttta atttatttca atcaatgcgc cattacggat atggctggca
    40081 aattcttgat aagtttcgtc aaataaatca taatgatcac gcaaggacgg atcaaccatt
    40141 tcttttggaa agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc agcaacaagc
    40201 tgactaacct gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc gatttgtgtg
    40261 cgatgtctgc cttgttctgg gcgataaaaa tcataaggta gatcgtagct agaattcaca
    40321 gcagtgtaat aaacaggatt atatccgacc ttttctacta acaaagtgag ttcttggact
    40381 aattttgaat cccggttgcc agaaaaagta gcagatttca gtggtttacg attcaaaaaa
    40441 cgtttagcta aatcatttaa aataggatca gcagattcag accattgagt aaagtaagtg
    40501 cccaagaccc catcatcaag tttgaggtat tcttctaaag taaagcttcc gtcaagaaat
    40561 ggcagaagta attgagaatg gagttcaaaa ctgctggcat cttcttgata caactcgtgt
    40621 gcgcgatgaa gcagatgctc caaaattact tccataccac gggaaacagg atggaagtac
    40681 acttgcacat acatttgata tcggctaacg atataatctt ccactgcatg catgccactc
    40741 atggaaaaag cgatcccgcc ttcataagga cgaatcacac gtaatattcg ggtaagatca
    40801 aatgttccat attctgttcc agtgaaataa gcatcccgaa gcaaatagtc cattcgatct
    40861 gcatcgatct gactagaaat catttggaca acttgtgggt tggggtaagt tttttggatc
    40921 acactagcta ccttttcagg aaaaccttct tctactcggt taagaatctg atacacttct
    40981 gtttctggag aagtgatgat ctctacagtg atggcttcat gattcgtatg gaagatatgt
    41041 tcaaatgtat gagagtaagg accatgaccg acatcatgca acagtgccgc acataaagtc
    41101 accagccgtt cctgatcatc ccagcctccg ttgcctattt tttcttttga aaaatttcga
    41161 gaaaagatat cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg agtaaaacgg
    41221 ctatgttctg ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat gcgtcgtaga
    41281 cgttggactt ctttcgaatt gatcaaatct aagattactt gatgctggac atggatatag
    41341 ttatggactg gatcacgaaa aactttttcc ataggcagca tttgatattt ataaggggtt
    41401 gtcataatgt agctccttta tgttattacg ttgttccatt ctctggattt ggttgttcca
    41461 ttgctccagc tggatttctt tttttagcca gtcggtagaa tcctgtaaat cacttgcaga
    41521 atacagcttt tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa aaaggggttg
    41581 gtcaagcagt tcttcaagtg ttgccattgt agcaggatcc actgcaggat agccatttgt
    41641 accaaagtct tcttttaaag cagattgata aaatcgcttc atgacttgcc cacgtttttc
    41701 ctgattaccg ttgacactta agtacatcat aacagaaatc ccatttttga tccgacgctg
    41761 agcgataccg gcaaattttt tgccatggat gcttaaatca taagttccag gacaataaga
    41821 atgggtgatt tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag tcaataggag
    41881 catttgctcg taagcgtcat ctatggttat tttttttgtg tcactttggg ggaaaatcaa
    41941 agaaatattc aaaattcctt catctgatac gatccctaat ccacctgaat ttctgatcac
    42001 tggagcgtaa cctgcatctt ccagtgtttg gatcccgtca cttagaaaag gagttcgaac
    42061 atccttcata cctaagatga ctgtgcagtc atattgccag aaatgtaaaa agatttgctg
    42121 gtgttcttta gaatattccg ttaatatatc tgttaatgca aaaggggaaa agtcgttttt
    42181 ttgataaatg tgctgatcta gtcgaatcgc agggttcatt caaccggctc ctttgtataa
    42241 attcatattt tctacaatat tatactctaa aatcaaagag ttgttttgga aaaaaggttt
    42301 tctgtaaaaa tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa tgtagctatg
    42361 agaagtaaag gagaatgaat aatggaatta tcacaaggaa caccgatttc aatcaaattg
    42421 cgtacaaaag taaaacagca aggggaagtg caagattttt attttgattt gaaagggcaa
    42481 atggtgacaa ttggcgatac gctgtacata cgttataaag agattcagga ggagacgggc
    42541 gatgagattc ctgtaacgat caaattagtt ccagatggtc atgtacagct gatccgtgca
    42601 ggagaaatgc ggatgcgttt gaaatttggc tataaagaac ggttagaaac atcctatcga
    42661 acaccatacg gtatgatcca gattgctact tttacgaaag aactgcatgt tagtttgaaa
    42721 gaccgtccca cagcagggaa ggttcgaatc gattatgatc tgttcatggg accggaaaaa
    42781 atcggcgaat attatttgac cttggatttt acagcgtaaa tataaaagaa aagtttgatt
    42841 ttttgtgtct gattttgtat gataaggagt agactgtgaa aggacgtgtc ctgattggaa
    42901 attagtgtat ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt agcacacgcg
    42961 atcttagaac aacgtggcga tgtcatggat ttttctgatt tagctaacca gatccaaaac
    43021 tatcttggga aatcagacag tgaaattcgt gattcactcg cacaatttta tactgacttg
    43081 aatattgacg gcagctttat ctcattaggg gataatcgtt ggggcttgcg ttcatggtat
    43141 gctatcgatt cgatcgatga agaagtaaac catggaatgg acgaagagga tgaagatact
    43201 ccacgtcgcc gtaaacgcaa gaaagtcaac gcctttatca atgatgatga agacgcgatc
    43261 gattacaatg acgatgaccc agaagataca gatctgacag aagaagatga cgatgatctg
    43321 tttgatgacg atgatgatga agatgaagaa atcgctgctt ataattctga cttgcaagaa
    43381 atcggagcag atgacccgac agatgatgaa gaagacttgc caggaaatat cgaagaagat
    43441 ttaagtatca tagacgaaga cgatgatgat gaagattatg aagaggaaga attttctgac
    43501 tctgaaaaag aataaacatt attttcttac gagaggttgt gacagagacg atcagcctat
    43561 cagaataagg aggtgtgaca cgattttt
//
LOCUS       DADXTG010000008        43172 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.008, whole genome shotgun sequence.
ACCESSION   DADXTG010000008 DADXTG010000000
VERSION     DADXTG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.008"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..236)
                     /locus_tag="IWI57_000340"
     CDS             complement(<1..236)
                     /locus_tag="IWI57_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBC2448510.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSD"
     gene            468..1460
                     /locus_tag="IWI57_000341"
     CDS             468..1460
                     /locus_tag="IWI57_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2448511.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1520..2941
                     /locus_tag="IWI57_000342"
     CDS             1520..2941
                     /locus_tag="IWI57_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBC2448512.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2934..4433
                     /locus_tag="IWI57_000343"
     CDS             2934..4433
                     /locus_tag="IWI57_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBC2448513.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4472..5866
                     /gene="uxaC"
                     /locus_tag="IWI57_000344"
     CDS             4472..5866
                     /gene="uxaC"
                     /locus_tag="IWI57_000344"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBC2448514.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            6025..6708
                     /locus_tag="IWI57_000345"
     CDS             6025..6708
                     /locus_tag="IWI57_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBC2448515.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6710..7546
                     /locus_tag="IWI57_000346"
     CDS             6710..7546
                     /locus_tag="IWI57_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBC2448516.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7557..9311
                     /locus_tag="IWI57_000347"
     CDS             7557..9311
                     /locus_tag="IWI57_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448517.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9587..9928)
                     /locus_tag="IWI57_000348"
     CDS             complement(9587..9928)
                     /locus_tag="IWI57_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBC2448518.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9996..12167)
                     /locus_tag="IWI57_000349"
     CDS             complement(9996..12167)
                     /locus_tag="IWI57_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBC2448519.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            12336..13235
                     /locus_tag="IWI57_000350"
     CDS             12336..13235
                     /locus_tag="IWI57_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBC2448520.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(13306..14163)
                     /locus_tag="IWI57_000351"
     CDS             complement(13306..14163)
                     /locus_tag="IWI57_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2448521.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(14148..16850)
                     /locus_tag="IWI57_000352"
     CDS             complement(14148..16850)
                     /locus_tag="IWI57_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBC2448522.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(16863..18152)
                     /locus_tag="IWI57_000353"
     CDS             complement(16863..18152)
                     /locus_tag="IWI57_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBC2448523.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            18302..19348
                     /locus_tag="IWI57_000354"
     CDS             18302..19348
                     /locus_tag="IWI57_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2448524.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            19350..21503
                     /locus_tag="IWI57_000355"
     CDS             19350..21503
                     /locus_tag="IWI57_000355"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBC2448525.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(21976..23427)
                     /locus_tag="IWI57_000356"
     CDS             complement(21976..23427)
                     /locus_tag="IWI57_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2448526.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(23424..25148)
                     /locus_tag="IWI57_000357"
     CDS             complement(23424..25148)
                     /locus_tag="IWI57_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBC2448527.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(25141..25755)
                     /locus_tag="IWI57_000358"
     CDS             complement(25141..25755)
                     /locus_tag="IWI57_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBC2448528.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(26100..27557)
                     /locus_tag="IWI57_000359"
     CDS             complement(26100..27557)
                     /locus_tag="IWI57_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2448529.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(27598..28518)
                     /locus_tag="IWI57_000360"
     CDS             complement(27598..28518)
                     /locus_tag="IWI57_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2448530.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(28530..29477)
                     /locus_tag="IWI57_000361"
     CDS             complement(28530..29477)
                     /locus_tag="IWI57_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2448531.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(29956..30675)
                     /locus_tag="IWI57_000362"
     CDS             complement(29956..30675)
                     /locus_tag="IWI57_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBC2448532.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(30724..32370)
                     /locus_tag="IWI57_000363"
     CDS             complement(30724..32370)
                     /locus_tag="IWI57_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBC2448533.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            32549..33139
                     /locus_tag="IWI57_000364"
     CDS             32549..33139
                     /locus_tag="IWI57_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2448534.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            33231..34736
                     /locus_tag="IWI57_000365"
     CDS             33231..34736
                     /locus_tag="IWI57_000365"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBC2448535.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(34820..36172)
                     /gene="celB"
                     /locus_tag="IWI57_000366"
     CDS             complement(34820..36172)
                     /gene="celB"
                     /locus_tag="IWI57_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBC2448536.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(36172..36690)
                     /locus_tag="IWI57_000367"
     CDS             complement(36172..36690)
                     /locus_tag="IWI57_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448537.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(36706..37032)
                     /locus_tag="IWI57_000368"
     CDS             complement(36706..37032)
                     /locus_tag="IWI57_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBC2448538.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(37045..39024)
                     /locus_tag="IWI57_000369"
     CDS             complement(37045..39024)
                     /locus_tag="IWI57_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBC2448539.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(39045..39359)
                     /locus_tag="IWI57_000370"
     CDS             complement(39045..39359)
                     /locus_tag="IWI57_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBC2448540.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            39527..39820
                     /locus_tag="IWI57_000371"
     CDS             39527..39820
                     /locus_tag="IWI57_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBC2448541.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(39898..41010)
                     /locus_tag="IWI57_000372"
     CDS             complement(39898..41010)
                     /locus_tag="IWI57_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBC2448542.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            41397..41576
                     /locus_tag="IWI57_000373"
     CDS             41397..41576
                     /locus_tag="IWI57_000373"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448543.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            41770..41940
                     /locus_tag="IWI57_000374"
     CDS             41770..41940
                     /locus_tag="IWI57_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2448544.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(42364..43104)
                     /locus_tag="IWI57_000375"
     CDS             complement(42364..43104)
                     /locus_tag="IWI57_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2448545.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        12987 a         8734 c         7744 g        13707 t
ORIGIN      
        1 atgtccgaga aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt
       61 ttccattcaa cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc
      121 gattcgatcg attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg
      181 gctcctaata aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc
      241 ttcctctcct ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta
      301 attaaatgag cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat
      361 ttttaacgtg tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat
      421 atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca
      481 aagatgtcgc taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca
      541 gcagtttaat aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt
      601 attcgccaaa ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat
      661 ttttctcaag tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca
      721 tccattcagt attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt
      781 taacagaaat caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg
      841 atcagccttt catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc
      901 acatcaatga tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg
      961 cagtagatta cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa
     1021 attttcgttc gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca
     1081 tccctgtcaa tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa
     1141 acatggaata cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg
     1201 acatagcgat tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca
     1261 tttctctgat cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca
     1321 ccgtacgtaa gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga
     1381 tcgaaggcag gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg
     1441 agtcgatcaa ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata
     1501 acacagaaag gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc
     1561 acctgtaaaa gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca
     1621 gattcagcag ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt
     1681 agaacaaggt cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca
     1741 aggactcagc aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat
     1801 gatcaatcct tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc
     1861 ctatatcgtc tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac
     1921 agatactccc ccacagagcc tcccgggcaa actgacagcc tttttatacc gacgtttcaa
     1981 attaaacaaa gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg
     2041 tctaaaagaa atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga
     2101 gtggttagaa aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta
     2161 tcctcgcgaa gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt
     2221 caaagcagaa ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact
     2281 accattcaaa caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga
     2341 acgtaaggtg catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc
     2401 ggaattggaa actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca
     2461 gttattcaat ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta
     2521 tgctgaccaa atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat
     2581 ttcattaaat agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat
     2641 cgaagaacaa aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat
     2701 gtatcgaggc gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga
     2761 tgcttggtct gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg
     2821 agaagatttg tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat
     2881 cgatcaaaaa ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg
     2941 aaaccctgca accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc
     3001 ctattccaaa acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata
     3061 tcccgcaagg tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat
     3121 atggctatcc aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc
     3181 ataatgtaaa aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc
     3241 caagtcatta cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag
     3301 tagggattcg taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg
     3361 aactgatcgt caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta
     3421 cgattttaaa gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag
     3481 aaattttagc ggacgcagta aatcatccca atgccggagg agttttagta tttggattag
     3541 gttgtgagaa caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac
     3601 gagtgaaatt tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat
     3661 tagaagagct tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt
     3721 tgaatgtcgg tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt
     3781 tacttggtgc attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag
     3841 tgccagaaat gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt
     3901 ttgactcaat cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac
     3961 cagtttacga aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata
     4021 agtctttagg ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg
     4081 gcgaaaaaat cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag
     4141 ctgcctctgc tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga
     4201 ctccgtttgg ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac
     4261 gcaaaggtca ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa
     4321 tcttagaatc gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg
     4381 aacaaaatga cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat
     4441 aaaacagtat aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa
     4501 agacgattgg gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta
     4561 tcactgtcac ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga
     4621 agcttggtta ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga
     4681 agaaaaaatc acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt
     4741 accaaaaatt atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt
     4801 cggtattgac ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga
     4861 aattttggca gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt
     4921 gatttgtaca acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga
     4981 agaaccagat ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa
     5041 agaaggattt aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac
     5101 ctatcaagaa ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg
     5161 tcgtgtttcc gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt
     5221 agaagagatt tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc
     5281 ttatcgtaca gaaacactga tacatttgac ccacttctat cattctcata attggacgat
     5341 gcagctgcac atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc
     5401 cgataccggc tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt
     5461 gaaccgtgca gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa
     5521 tgactatcct gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact
     5581 gcagttaggt tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac
     5641 acaatttgct gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag
     5701 tttcctgtcc tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga
     5761 aatggtcatg cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc
     5821 gattagttat acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa
     5881 cagtaacgaa tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag
     5941 aagaagacgg accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata
     6001 aacctttatg aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg
     6061 gcagaaaaag atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat
     6121 ttttcacccg agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt
     6181 atccaggaag gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt
     6241 gtacaatttg gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt
     6301 agctatttaa aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg
     6361 gtcttactga catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat
     6421 atacttggag actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt
     6481 ttagatgtgt tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa
     6541 aaattctacc tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac
     6601 aatacccact ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata
     6661 aaagaatccc gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt
     6721 aaatttaggc atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa
     6781 agtatcacag cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca
     6841 acttaaatta actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa
     6901 aaacaatatc gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga
     6961 agttcgtgca caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca
     7021 agccaagatg attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt
     7081 tactgaagaa gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga
     7141 aaattttggc gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca
     7201 gttagctaaa cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg
     7261 tgcgaatttg atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt
     7321 ggacaattta catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg
     7381 aaagatccgc atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta
     7441 tatcaaatat gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt
     7501 tcctgaagca atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg
     7561 aggaacgaat acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc
     7621 atctttatca gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac
     7681 atcttccatc ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga
     7741 aagattcttc tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg
     7801 gtcaaaatat attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag
     7861 tagaagctgg actaagagcg acccttatca agaaaatcca agaactatct attccttatc
     7921 aaaaagaatt gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc
     7981 aaacgttgtc ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag
     8041 ctttaggtat cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc
     8101 ctctcgccgc attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact
     8161 tcaggcaaga aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga
     8221 ttgctcgagc tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc
     8281 aaatctatga aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct
     8341 gggctagttt tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac
     8401 aagggcgcat ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca
     8461 attccgtttc tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca
     8521 cttctattgg cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa
     8581 tccaacattt ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac
     8641 tcaaaccgat tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg
     8701 ttgggccatc tggctctgga aaatctacac tccttaactt attgattgga tttatccagc
     8761 cgacttcagg aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg
     8821 tgcgaaatta tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag
     8881 agaacatcac ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag
     8941 ctgcgcaact ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg
     9001 aacatggcaa caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga
     9061 tacgtcagcc aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag
     9121 agaaaaaaat ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg
     9181 ctcatcggct ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa
     9241 tcgttgaaat aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga
     9301 aaaatgaatg acaaaaaata ggagctatga caagactttt gtcgtagctc ctatttctga
     9361 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcat caaacatgag
     9421 aagctgtttt ttgaaattgt tccttgtaac tcagagttaa acggtaggct cacgacctct
     9481 tggtaaatgg tgttcaagag acagcctatc ggcaataaga agcaattttg tcatcgctca
     9541 aggccgaacg tctctttcac accctctgtt gtttcataaa atagttttat tcgctgtaaa
     9601 ggtcgcgaac tggtgtcatg atgatacggt tcaagtcatt gatgattgta ctgaagcctt
     9661 gctctttttg catcaagtca ttgattactt cttctgattg gattttcaca gccatttctt
     9721 gtgctttcat tgcatcttcg tcgctgaact cttgtccact catttgtttt tcttgtaatt
     9781 cgatttgcat cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt
     9841 tctcgtacgc ttctttcaac gctttgaatt ctggtaattc acggatctca cgttcgattt
     9901 gattcgcgct atcgtagatg tttgacatgt tatgccctct tttcttttag tagttactct
     9961 ctattgtaac atggggttat tcgttttcaa aggggctact ctccttgata tttccgccag
    10021 aggtctttag ccgcttcacc aattccctga acgccttctt tcaccatatc gccgaagttt
    10081 ttcagccctt cttttgcttg ttcaccatat tgattcaagt tatcttgcca atttccagac
    10141 tctccattag aatttcctgt gtttccaact tgatctgcag ctaccacttg tcctcctgtg
    10201 gcataggcat ctgctacact aaatcctgtt tttgaagtat acggaaggat gccttctgcc
    10261 gctgatttga agactttccc gacaacattt ccacttgtcc cttccaaata gtgcagcttg
    10321 ctggattctt cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta accgatcatc
    10381 cattggtcat ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt tttccctgct
    10441 atcgtataac ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt accaagaagc
    10501 atgctggtca tttcatcggc tgtttccttt gatatgacct gttttggttt tttgtttgtg
    10561 ttatcgacga tcaccgctcc agtcgaatca acgattttcg tgataagatg cggcgtatac
    10621 atctgaccat cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg agatactcct
    10681 ttttccagtc caccaagggc taagccataa tacttatcag attttgctaa aggcaggccg
    10741 aattcttctg ctttatcata tcctttttgt aaaccgatct catgcaaaag ccatacagta
    10801 ggcaaattca aactttgtgc aacagcttga tacatcggta cttctccttg gtatgtcccg
    10861 tcaaaattat gcgcatcgta ataactttgc ggttcgtctt tcaagatact ggaaggcttg
    10921 tatccagctt ccagtgctgg tgtatatacg cttaatggtt tgatcgttga tcctggtgaa
    10981 cgacgcatct gtgtagcaaa gctgaatcct ctaaagacat gatctcctct tcgtcctact
    11041 agtgcttgta caccacctgt attcggatct aaagctactg aagcagattg gaccatggca
    11101 ccatctgaag catttgctgg gaataatgca ctgttttgat aagtagcttc taattgcttt
    11161 tgataatcct gatctaaagc tgtatagatt ttatagcctt tattcatgac atcttcttca
    11221 tcaagaccat actcgttaac tgcttcatcg atcactgcat caaaataata aggataacga
    11281 taaccagaat ctgaattatc gtatgtgtca ttaagatacg cgctgatatc tgtctggctt
    11341 tctgcttgtt cttgttcttt tgtgattttt ccgttttctt ccattacact tagtactgtg
    11401 tttttccgat cattcgcatt ttttgggtga tcgatcggat tataaatacc aggacctttc
    11461 agcattcctg ccaaagtagc agcctcagac agcgtaacat tcgttgcatc tactccaaaa
    11521 tactttctgg aagcgtcttg gacaccccat accccgttgc caaaataggc attgttaaga
    11581 tacatggtca agatttcttc tttgctgtac ttcttttcga tctcgattgc caaaaataat
    11641 tccttcgctt ttcgatcaaa cgtttggtct aatgtcagat acgcattttt ggcaagctgc
    11701 tgagtgatcg tacttcctcc gccccctgat gttccgccag acaagaccat tcggactgct
    11761 gcacgggcaa tccctttgat atcaaacccg tgatgctcgt aaaaattcct atcttctgtc
    11821 gagattactg catcttggat atagggagaa atcgcgttta attctacata tgttcctttt
    11881 tgggcgtaca gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt cgattggctt
    11941 aatcctgatt ttaacgtttc gacatttgct tgttttgcta aaataaagag gtagatactt
    12001 gtcactaaga cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc
    12061 cagatgcgct ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa
    12121 ctgatcaaag cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc
    12181 tgtctatttg attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta
    12241 agttgtagtt caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag
    12301 aaaaatgtaa gttttttctt aaatatgttt taaaaatggt acaattagtc tgcgattgca
    12361 ctgaaaggag tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa
    12421 caattcgcga cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc
    12481 gtatacgtaa aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg
    12541 gcaatgacca gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat
    12601 taggagatgc gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata
    12661 agcctgcagg aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc
    12721 atttagctgc ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca
    12781 aagaaacaag cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa
    12841 gaatgttgga acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg
    12901 ccaaagatat gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg
    12961 tcgtagatga taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt
    13021 ctgaacaaac acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc
    13081 atttagctca taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg
    13141 aacgactgat gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa
    13201 tagtcggtaa agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc
    13261 agcagataat gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc
    13321 gtgttctgct aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac
    13381 gatatattca cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg
    13441 acggattttt tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc
    13501 aggtgctaac gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc
    13561 ccaaacagga tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga
    13621 cggtcctgct gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg
    13681 gtcatctgga tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa
    13741 aatttcttca ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca
    13801 actatttttt ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc
    13861 cgttgtccaa gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg
    13921 tgcccaccct tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat
    13981 accaatcggg ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc
    14041 aaatacctga tccaatgttt cttctggtgt tgtggtaggg atacttaatt tttctaaaat
    14101 ttctccacca tctgaaaccg cacacacaaa ttttgttccg cctgcttcta ttccgccata
    14161 cattcgtttt cctcctcgaa tccgattgtc cgaatctcat aaggttggat tattgggaca
    14221 accgcttgct tcgttggttc ttcaagcaga ttcaatatga cagtttttcc attgtcttta
    14281 gttatttcca atttttctaa atgactagac atattgaaac ctcttactac tactttatca
    14341 ctaaacttgc tgcgtttcaa agctgtaatt gcaaacgttt cactttttat cgttaaatat
    14401 tgttgttttg aaatcaatgt tccttcatga tgtttgattt gttgagtgga aaaaggaatc
    14461 tgtgctgcgt atgcatgcaa ataagtagag aatttttctt ctggtccatg tagttcaatg
    14521 ctgtaattga accgatgctc acctagacac tgtgcttctg gtgttggaaa atatccccaa
    14581 tcgcctagtt ctcccacact gcgaagtagc gtcactgcga tttgtccgtc ttctgttact
    14641 tcatattcat tcaaaccaaa gtttccgact gttacaccgt actcttcatc atgaatattg
    14701 acaaatgctt gctgatgttg cggattcgtg gggttttccc aacttttaga cacatggttt
    14761 gggcgcttca ccacttcaaa aatactatct gcttcgtgat gttccacatg taactttgta
    14821 ggaaacaaga cacgtaagcg atgatctttc atctgattgt cgagagtcgt ttcaaagtcg
    14881 atttttttac tatcttttct gattgtcatg attgttttta tttgaaggat tcgtttttct
    14941 ttggatcgtt ctgcttttcg ataacgaaat tccacaacca tctgctgttc tttttctaaa
    15001 cgctcgtctg cagaaacagg gatttccatt tcctgtagaa tacttatttt tgctatttcc
    15061 ggtgtatcta ctaatacttt tacttcagag ttttctttgt tactggatag gattggctga
    15121 tcacagaaag gttgtttaaa gatatactca tttcctatat cgccgatatc ttcaaaaata
    15181 tgcagattat tcaactttcg tttgtttttg cgatcttcca ttgtaatggt accatttttt
    15241 tcaatcgtca aatgtagagg gccgttttcg atgattcgtc cactctgatg aatcattgtt
    15301 tccttttctt ctgctttcgc ttccccttct acaagcgcga atgtttccca agaaaatgct
    15361 ggcatgtttt ctaatggtaa agtcactttg atcattcttg ccatatatgg cacacggaaa
    15421 cggtctttcg gcaaatcata accaaattgg acagtttctt cagataaaat agctggtata
    15481 gtggccccac tttttgtttc tacatgatac ttacgtttag gcaaattttc taactcttga
    15541 tatagttgtt caggtattcc ttcagcgaat ctttttcttt ctaattcgat ggttatttct
    15601 gcttcaccag ttttatccat tcctgcagta tttacaatga caaatggaaa gctgtctttt
    15661 gggaatccgg tcgtatcgat tgctgcggtc agctgttcta aggaatcttg tgcaagatac
    15721 tttccaactt catctgcttt ttcaaaacga ggtatcattt cccgatggac agaatcgact
    15781 gaacaaccgc aaatactgtc gtgaggatga ttttgcatta atgtcttcca agcataatct
    15841 aattgatcat ggggatagtt tccactaact tcataagcca tcgtcgcaag tggttctgta
    15901 atattttcta gctgtcgctg gaccttggta ttccattgtt ttaagtaaac ccgagcagat
    15961 gctgtattgg ctaaagtata ccagccatcc gtttcttgac ttgtcaattc tccttctact
    16021 gagcctaagt cttctggcac atctttttga actgcttcca gatagtcagt aaaattagaa
    16081 tgaataaact cgtaatctgg aaacagttca tttgcgagat ggattgcttt tgatatatct
    16141 ttttgtaccg gttgatgatc aacaccattc atcatcaata agtggttcgt agaggcatat
    16201 tgttctgcat ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt ttctactgga
    16261 atctcattcc cgttgctata ccaattggca aatagcagac caaagattgc tgtttgatca
    16321 ggtcctttcc accacatctc tgagtattgg gaagaatagt tttctgcttc caaaacttga
    16381 ttatcaaagc caattggttt tacaccacgg ccaaacgctg ctgctgaaat accagcttgt
    16441 ttcattaatt gaggcgtctg tcccatattt ccaaatgtat cgggaaaata gcctaacatg
    16501 acaggggttc cccatttacg gctttcttcc atgccaatca gcatatttct cacgttcgat
    16561 tccgagctga taagaaaatc atcttgcaag atataaaatg gtccaattct caattttcct
    16621 tcattgattg cttgttggat tgcctgtctt ttttctggcc ttacttgaag atagtcatct
    16681 aaaataatcg tttgcccatc caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc
    16741 tctagtagat cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta agccatgtac
    16801 cattcacgat cccagtggct atgagaaatg atatatactt tctttttcat ttacgatacc
    16861 tcctactttt ctactctaat atcaaagtaa tccatgacta attcgcaaaa catcatattt
    16921 gcccaagaaa accattctct cgtataattt tcaggattgt tcacatcgaa tccttcgtgc
    16981 ataaggtgtg ttcctgcgtc agttgctaca agtaaattca atatccgctc tttttcagat
    17041 ttgtctgacg ttgtcattcc ttccatagct aatgcaatcg gccaaacgta tttttctggc
    17101 gtatgggagg atccaattcc ttttgcgtac tttccttcat agaaatatgg attttcttta
    17161 ctcaacaatg ttcttcttgt ttgcagataa tcttcctctt gttcagaaca atatcccaag
    17221 taaggtgcgg cgatcagatt gggcacatta ctgtcatcca tgactgtggc gtttcccatt
    17281 ccatctactt catatgcata aatcgtttgc cctttttgat taaccgttcg tccgtgattc
    17341 tgaatcccct cttggatctc tttcttcaat ttctgtgttt gctgtacaat ttctttatca
    17401 tcgagtatcg tttgaaaaat ttcttccaca taacctaaca cgaccaccgc aaacatattg
    17461 gatggtacca gatatccata acggcaagta tcatcgctag gacggaaacc agaccatgtc
    17521 attcctgtaa aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt
    17581 gtcgtgtctc gtacaaatgt ataaggagag tgttcatgat cttgctctgt tttgaacacg
    17641 tccaagattt ttttgatacc ttcaataaaa gattgattga attgatcagt ttttcctgta
    17701 tttttgtaaa gaagataggc caactgtatt gggtagcata aagaatcaat ttcatatttt
    17761 ctttcccaga tccaatcgtt catttttgta tgatctgttt ggtggccagc accgttagct
    17821 tcttcgttga aggcattcgc atatggatcg atattgatat aaaaaaattg ctttttgact
    17881 aacccgctga tcatgtcagc taattcctca tcttcttttg caatcactaa ataaggacga
    17941 acttgtgcag tagagtctct taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg
    18001 gttccgtcat tttgcttttt gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa
    18061 ttctttgccc agtccgcatg gatatcccca catttttgtg tgatatcttc cataaatttc
    18121 tgcaccgatt ttggtatatc tttataagcc actaataaaa ccttctttct atttgatata
    18181 tctttaaaga ttatacttca tacagccaat tatgtctacg ctttcaaaaa atatcttttt
    18241 atttactttt tcatccatta tggtattatt tgaatatgat atatcattaa gaggtgttta
    18301 tatgaatcaa ccattgtacc agacaatatt taatgatttg caaacggcta ttttaactgg
    18361 tgaattacct gtagacagcc aactaccaac tgaaaaagaa ctttcacgta cctataaagt
    18421 aagcagaatc acttccaaac gtgcattgac tgaattagag caattaggac ttatttatcg
    18481 cgttcgcggg aaaggcagtt tcgttaaatc cgcttctccg gaacagattc ttgcttctcc
    18541 tacaaaaaca cgacggattc tgtttcttct tccatatgtc gctgatttgt ctgttggtga
    18601 ttttagcaag ggattgaacc ctgtgatgca agataaacag attgatgtca tgatgacgac
    18661 gcttgacttt ctacaaaata aaacagcaaa agagctaatc aatgaatttg acggattgat
    18721 ttattatgct cttgatacag aacaacatct tgacttactt tttgagcttt cattgaaaga
    18781 attccctgtc gtcatattag ataaaaaaat ctatgagtta ccttttccta ccgttctttc
    18841 cgataatttc caaggcggtg cgcttgctgc ctcttatttg atcgaacagg gacattcaaa
    18901 aatcgcttat ttgtttggta cgcagactca tccacaatcc gttcgacagc gttaccttgg
    18961 ttaccttgaa gcattggaca aagctggttt gactttccac acttcactgg atgacaaaag
    19021 cgcgacatta gatgaattgc tttcttatat cactaagtat gaagtaactg cttttgtttg
    19081 tgaaaacgat gttgtcgcta tccagaccat gaatctcttg agacagcatg gttatcatat
    19141 ccctgaagat ttttcaatta tcggatttga tgatatccaa gcagcagctc ttgttgaccc
    19201 gccactgaca accatcgcac aagattttga acagttaggt aagcttgcgg gagatgcttt
    19261 ggtcacttgg ttagagacaa aagatcgacc atccgatagt aaatttccag tttcattcat
    19321 caaaagacaa tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat actagacatg
    19381 caacagcgaa tcagccaaat ttctctaatg ggaactgcca gccttatact ggcgttcctt
    19441 ttgggatgaa ttattttgca ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc
    19501 ctgatgaccg cgtttttcaa ggctaccgtc ttacccatca accaagtcct tggatgggtg
    19561 atttcagtca tatgctgttt acgccgatca atggaaaact acaggagaac acccttttcc
    19621 atgctcaaag ttcttaccgc ccagaagaaa gtatattttg cccgactcat ctgtcgatct
    19681 gtcaattacg ttatggcatc tgttctacat tgatacctag catgtatggc ggaatcttat
    19741 ctattgatta cagcaaaaat gacagcggtt tacttttatc ctttccggga cgccatcagc
    19801 tctttgttat agatccttac acagtggaag gaaaaatcac acattttgct ggttcagaag
    19861 atcctgattt ttctttttat tttatattga gatttgaata tcctttaaca attgatgagt
    19921 tagctgtttc caacaaaaat agcgactcga tcccgttgta ttttgaacag acaaaaaaac
    19981 aaactatccg atttgggaca tcttttatca gctcggaaca agcagcttat aacctggaac
    20041 tagaacaaaa ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa tggcagtcct
    20101 attttgatcg tatacagctc acacaccatg atcaacaaca attgcggacg ttttatcata
    20161 atttatatcg ggtgttttta tttccacaaa cattttatga aattgatcct agtgggcaaa
    20221 aaatccacta tgatacgact agtcgatcta taaaacctgg tgttctctat acaaataatg
    20281 ggttttggga tacgtataaa acggtttatc ctctttattc tttgattgct gtagaaaaat
    20341 acgaagaaat gctggaaggt tttttgaaca gctatagaga aagtggctat ttgccaaaat
    20401 ggctgtctcc tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg gtcattgcag
    20461 atgctgctgt aaaagatatc cgacgagact tgatgcctga atttctaacg gcaatggaaa
    20521 aaggtgctac ggttcaaagc aaacatccaa attatggacg ccaaggaaca gaagattact
    20581 tgacatatgg ctatgttcct aatcactacc acgaatcagt caatcacaca ttagactatg
    20641 cttatagcga tttttgtatt agccaagttg ccaaggtact agaagaagag gaaatgacga
    20701 agaaatatca gcagcaatca ttgaactatc aaaatatctt tgactatgag tcaggattta
    20761 tgcgttcaaa agataaagat ggcaagttca gagaaccatt ttcagctaat cgttggggac
    20821 aagattatgc agaaggaagt gcttggcaaa gcagctttgc tgtttatcac gatttttcag
    20881 gactgatcgg aaaatatggt ggcagtgaga gatataaaga aaaactcatt gagctgtgca
    20941 atcagccccc aacgtttgac ccaggaggat atggttttga gatccacgaa atgagtgaga
    21001 tggccgcgat agaatttgga caattagcta tttcaaacca gccaagtttt cactatcctt
    21061 acttgttcag ttatatagga caacctgaaa tggcacagcc actgcttaaa cagttgatga
    21121 ctcagacctt cgacgattct ccaatgggtt accctggaga tgaggacaat ggaagtatgg
    21181 ccgcttggta tatcttcaat agtctgggat tttatcctgt cacgcctgga accggtgaat
    21241 atgttatcgg tatgccatta attgacaagg ctgttttgct tttgtcaaat ggaaaggaat
    21301 tgacgattga agcaacgcct aatcggccgc agcaacaatt tatccataaa atcaaacgga
    21361 tggacaaaga atatacgaaa ttatatttta cccatcaaga tttgctgaaa ggcggcacga
    21421 tctcttacca attagggatc atccctcacc ctaagaaata tcaagcaaaa gatttgcctt
    21481 tttccttatc aaactataat taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
    21541 aagaacagcc aagcaaaaat agatcctcat atttttcgtt tggttggtct tattaccgca
    21601 gatgcaagct tttgaacacc gtttattgag aggttgtgaa atcagcgttc tgacctgagt
    21661 attagagaga caaatggcaa aatagttcct catattttgc cattttttga gctaataccg
    21721 agggtcagct gattgaacac cgtttagtgg agaggtcgtg aaaaagctgt ttagtttcga
    21781 gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata
    21841 ccgagaaact ggcttttgaa ctccgtttat cgagaggtca tgaaaaagct gccttgcctc
    21901 aagtaataag aacagcccaa aattagattt ctagacaatt ttttataagt tatttcatta
    21961 cattggcttt ttcttttatg attctccact gttttctcgg tactctttag gggatctgcc
    22021 gacgattttc ttgaataatt tagagaaata cgtggtgttg ttgtagccaa tctcacttgc
    22081 tatctcattg atgttttgat tactgtgcaa aagcagttgt ttcgcttttt ccatacgtaa
    22141 atggttcaaa taagctgaaa aacttttttt cgtttctttt ttgaacagct gtcctagata
    22201 catgacattt aaatgcaggt tttctgacac ttctttgagt gtaagtggtt cttgataatg
    22261 ctggtgcagg attacgatta ctttttccac attgtcactg tactttttct gtctctgatg
    22321 ctttttgatg aatgacagaa gtagctgctg aagttcttgc accgtttttg cctgatggat
    22381 tttttcgatt ccttgtaaat attcctcatc ttccagatca atcaattccc gttgaatctc
    22441 cataaacagt aaaaaactgg aatatcgtat atcttccggc atcatggctg ctaactgaaa
    22501 ctgttcaaaa aaatcagtga tcattttttg agcgacttcc aattgtttat tttgcagcac
    22561 gcgtctgaaa cttgaaaaat cgatggtttg atcttttgtt tctgcttgta ctgcgtaaaa
    22621 aacatgctcg tctttatctc cataaaattg atgcaactgt agattgtctt tcgcttgttg
    22681 gaatgtttca gggattttgt ccaaagaatc tgtctcttcc ccgatactga tcagccattc
    22741 ctgatcagga aagttttctt gaacaaaaca acagaagcca tcaacttcat ctgcctccaa
    22801 caacaacgtg atcaatacac ggtctccgta atttcgttga tagtaaaaga tttgttgccg
    22861 ttcttgcaac catctattga taagagtatc atgagttctt gccagttgaa tcaatactat
    22921 cctatgctgc ttctcactta tttgtcctat tatttcttct tcacttattt catccaattc
    22981 ttcattcaac cattgactga ataacagttc ttgatagatt tcttgttgat ttttttgttc
    23041 attttgctga tctaaccgtg ttttgatttt cttgaggctt tcaaccaatt cgaacttatt
    23101 cacgggtttc atcaaataat tcacggcgcc taattgcata cctcctttca aataatcgaa
    23161 ttcttggtat cctgataaaa tcataaattc aaaattatga cgttcttttt gagccgcttc
    23221 aataaattcc agtccattta tttctggcat tgtcacatcc gttactacta aatcaatctg
    23281 tcgctgttca agtacagcta gtccttcttt ggcattctct gctgttgcta ctatttgaaa
    23341 gcctaaactt tgccaatcaa ttatcttttt taacccattc aaaatcatgt attcatcatc
    23401 taccaataac acttgataca cgctcatccc tccttgaatg taatagaaac ggtcagtcca
    23461 cctgtctcat tattctcaag cgacatataa taactgctgc cgaagctggc acgtaaacgt
    23521 tcattcacgt tttgcagacc gatagaaccg tgaagttcca ctttaggatg agacagtttt
    23581 tcttcaatct gttttagccg tttttcagtc atcccttttc cattatcttt gatgatgatc
    23641 cttacccgtt tgccttcctg caagactttg acacttagcg cattatcgaa tcgagtaaaa
    23701 tcaataccgt gtttaaaata attttcaact aatggctgga tcacaaattt aggtacttcg
    23761 atcttttcca aatcaggatc gatcataaaa tgataggcta ctcgattagg ataacgcatt
    23821 tgatacaaat agacatattt ttcgcaaaag tccagttctt cttttaaagt gatcgttttc
    23881 tcttgattcg tattgtttcg aagcaaagcg gaaaacgcat acacaacatc tgctaattct
    23941 tcacttcctt cgctgattgc atacattcgg atatattcta atgtattata taaaaagtgt
    24001 ggattgatct gtgcttgaag cgcccgcata tgtgcgtctt gctgtttgat ttctaatttg
    24061 tagatgtctt ctacaaactg attgatgcta tccaacattt cgttgatacc gattgaaagt
    24121 tccttcagtt catattccgt gtttgttatg tcgatccgcg tctctaaatt ccctgctgct
    24181 gtttgatcca tggcctgcat gacgatagat acttgctgtg aatatcgttt gaatgtttta
    24241 taaagaaaga taagcagtat attgtctaaa ataatgatac caatgatcag cggggcaaga
    24301 ttttttcccg tttcgatttt tacacttttt ttatctagta cagctaatat acgataatca
    24361 tcagacagtt cttggtgttg aacaaaattg tttttttcca gatcgtcaat cggcaattgt
    24421 gaatttttct tcagactctc tgtgatgatc atttcttgtc tttcaaacgt ttctttagct
    24481 attttattat catggaatgt gtatagtcga tttgtcgttc cagaaatcat gtataaagag
    24541 agtccattga atgacgttag attttctaaa atcatatcta gttctttctt ttgaaaacca
    24601 ataaaaatac ttcctgactc agctcctcct tgtgtgatag gcaacttgat ataaaaggcg
    24661 tctagtaatt ttggtgtccc cttttttatt tccccacctt tgttttctct agaagattca
    24721 taatactccg gtagttgatt caacacgata tatatcgcac ttagttcttc ataatttgcg
    24781 tagagatctt tgatttgatt tgggaaagaa actaattcat cggtactgtt ttgctgttcg
    24841 tatacataca tcagatactg atctatgggt tttgttaagt aggtcgtcac attttcgatc
    24901 ttttcggaat tccctacaag ctggtttttg atgatcctac tcaaggttgt tttatcattc
    24961 agcattctgc ttgtgctttg aacggcatca cttgtcatgt tttctgcctg tttttgattt
    25021 tgttcccaag tatggatact aaaaataacg gtagcagtca tcgttacacc gatcatgata
    25081 accgcgtatt tccgcaacag acgatttagt aaggttcctt ttgaaaatag aatccgaaac
    25141 ttattcataa ctttccaact gtttatctaa ttcttcacgc caatgtgttg tcatcgtacc
    25201 attccaaacc gtcaaaagac caatgacacc aaacaggatc aatcctttga attgccaagt
    25261 cacgcctaaa attataccgc taccaataat tatttttaga aaagacgaaa agctggaaaa
    25321 aaccgaaata aaacttagct ttagcagatt aggaacagag atcgtatatt cactatctaa
    25381 aatcatactg tattgataag tagcaaatag gcagagcaag gcaaaaagta aaataaaatc
    25441 aatagctaaa aaaatgagcc ctttgatttg aacagataaa tataaattgt aactcaataa
    25501 agcagtacca gataaaaata aactaaatag cagattgcct cttaaaaaat ttcttttata
    25561 tatattccag cctcttttta ttgtgatttc tttatactcg aatccgtaca aataaaatag
    25621 ttcattgact gttttccatg ccgcacctaa tccaaagatg atcccgccgg caaatgtata
    25681 tatccagaaa tacaaggtta gttttataat gacccagatc cggataaata actcttccaa
    25741 aaccttccct aacatatctt ctcctccaaa cattaagttt atactttctc attatacaaa
    25801 aaaaggttgg gcaatcagca ccttgacctg attattagag aaacaaacgg aaaaatagtt
    25861 cctcatattt tgtcgttttg tgagcgaata ccgagggtta gctgattgaa caccgtttac
    25921 caagagtttg tgaaatagcc gcttagtttc gagtagtaag aaaaattttg tgaatatggc
    25981 tttccacatt tttacatata tttagcttaa taccgagaaa ctggcttttg aacaccgttt
    26041 attcggaaca agaggttgtg acacgtcttt tgtcacaacc cctttgtctt tattcattat
    26101 tattcttgtc ggaactcatc atattgtttt tgcatttctt tcaatacctt atcatagcca
    26161 gccttgtcca aagcgtcttt caatttcggt aatgtttcat ctggatcaac agtccctgta
    26221 ttcaatccat ctaaatattg attcatgaca ttagaaatat tacttaattc tgttttgaca
    26281 ctgtcagtgt tgaagctgaa tcctaaaatc ggtgaagttt ctgcatcagc aatcgattga
    26341 tcacgtttgg caatcatgtc atccgtaata gactcttgtg tatatagaat cttgttgttt
    26401 cctgtattcc aagcagacat atgtgtattt ggctgataac catctagcaa tttgatttta
    26461 tcatctgaac catcgacttt ttcccatgct tctccctcga tcccccaaac aagcccattc
    26521 aataattctg gatcactgtt aagcagccct agaagttcta ctgatttttc tttatttttt
    26581 gaagtatttg atacgacaaa atttgccatt tgtgcttgag ctgtagattt tagtggaaca
    26641 gtaattgctt ttgagacaag ttcttgtttt gctgcatttg tcaaaattgt atcgccataa
    26701 tcatacggtc cttgtgtttc tccacgcatt agccaagtat ttccttctag tggataatcc
    26761 ttattgctcg ttgcagcgtc acttggaatg agtccttgtt tgtaccagct atgcattgta
    26821 cgtaacagtc cgatatacgt ttcattatcg tattggttga tgattttcga atcatctcca
    26881 tccagatcaa tagcaaatgg taacgtattc cctaaaggat aatcaaagtt cccttggact
    26941 ttgaagcctt gtccaatggc aaaagctgcc gtgtttggtt ctttttcatg gaattccttc
    27001 aagacttttt ctgcatcagc gtaagattgg attccatcaa ttgaaagatc gtatttatct
    27061 agtaaagctt tgttaaaagt aagtacttgt tgagcaaaga catttccatt gactgggaaa
    27121 gcatataatt ttccattgac cagatttcct ttaatatatg cttcatctaa atcattataa
    27181 gctttttcag cgtacttagg cgctaattca gtcaaatcag caaaggctcc tttttgggca
    27241 ttggaaacat agttgttcgc aaaggcgata tcataatttt ccccggaaga aatgatgacg
    27301 ttcattttct tttcgtaatc gccccagcca atgtaattga tattgacttt tacaccgatc
    27361 tctttttcga ttcgtttgtt tgctacttcc atcaattgat cgaaattatc tggtttatct
    27421 ccgatttgat acatctgcaa agtgtccgaa ttgctagctt ctgcctggtc tttagaacca
    27481 gaacctccgc atgctgctaa tgttcccaac aacaacgctg aaacacctgt aaaggctagt
    27541 ttcttccata acttcatttt tcttcctcct aattttatga tttatatttt tgtttcttta
    27601 ttcttttact ccgccgattg ttagaccttt gacaaaatgc ttctggaaaa atggatagct
    27661 gatcgcaata ggcaacgtcg agatgactac gatcgccatt ctggcagatt ctcctggaac
    27721 tgaggctaat ccgcctgtca attggcttcc tgctcctgaa ttttgtgtca gatattgaat
    27781 attactttgg attttcatca aaagatactg tagtggaact agcttatcat cttggatgta
    27841 tagtaaagca ttgaaccagt cattccagta acccaatgcg gcaaataaac tgattgtcgc
    27901 aatacctgga acagctaacg gtaaaacgat ctgaacaaat attcgcagtt cgctagctcc
    27961 atcgatccgt gccgattcaa taatcgaatc agagaccgaa cgtttaaaga atgtgcgcat
    28021 cacaataata ttgaagggac tgacagccat tggcaagatc aatgcccaaa tcgtatcttt
    28081 taactgaaga agatttgtca taacaagata attggccacc atccctggtg agaacaacat
    28141 cgtaatcaaa caaaaaacgg taaaaaaacg acggaatgga aagtttgacc gtgaaatcgc
    28201 ataagcatat aaagaagtag ccgtgctatt gatcagtgta cctaaaacag taacaagtac
    28261 agtgacgaaa agcgcttgga aaattttatc ctgcatctgg tcgaacaagt acgtataacc
    28321 aaatatactg aacttttctg gccaaaaact gtacccgtga gtcgacaata tggactcatc
    28381 ggtaaaagag atggcgataa caaatataaa tggcaaaata caggaaatag caaagagtgc
    28441 aatcattata ttagagaaaa aattagccgt cttattgaaa gagcggactt ggactctctc
    28501 cactttcttt tttttcattt ttttcctcct tagaataatg cagaatcgct atcaaaacgt
    28561 cgggcgatcg tatttgtgat catcaacaag acaaagccga caactgattg gtaaagtcct
    28621 gcagcagccg tcataccaaa atctcctgtg gatgtcaggc cattgtagat atacgtatcc
    28681 aaaacttgag tcacatcata taatgcaccg gaattcctag gaatgttata aaacaggcca
    28741 aaatcagctc ggaaaatgtt tcctacggct aaaatcgtta aaatcgtcat tagtggaata
    28801 agttgtggta tcgtcacatg tttgatctgc tgccatttgc tagctccatc aaccatagca
    28861 gcttcataat aagtcggatc gattcccata acagatgcaa agtaaataat gctgttataa
    28921 ccaatccctt tccatgtacc aatgaacacc aagataaacg gccaatattt cggttcatta
    28981 taccaattga cagcttctcc tccatgtcca gtgatccact gattgaatat tcctttgtca
    29041 ggacttaaaa aagcgtaaac aaagtaactg ataatgaccc aagatagaaa ataaggtagt
    29101 aaggacatcg tttgataaac tttaacgagt cgtttatttc tcaactcgct cataatgatt
    29161 gcaaatacga ctgagatcag cagattcaaa aggataaatg ctacattata taaaattgta
    29221 tttttagtga tgagaaatgc ttgatccgaa gaaaataaaa atttgaaatt ttcccaaccc
    29281 acccaaggac tttccctcaa gctagccaag aacccatctg ctgaaatatg aaaatttttg
    29341 aatgctacga cattcgccat tacaggtatg taaaagaaaa aaatcatcca aagcataccc
    29401 ggtacagcca tcaggacaag tgctttataa cgccatagat tcataaaaaa atgattgaac
    29461 ccctttcgtt ttttcatcta aaaacccctt tcgattcatt cgcaacgtaa ttatagcgct
    29521 ttcaataatg agaataaata aacaaagtat agggaaaatg aaacaaagta taggagaggt
    29581 tgtgaattca gcgttttggc ctgagtatta gagaaaaaaa cgggaaaata gttcctcata
    29641 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcagaggt
    29701 tgtgaattca gcgttttggc ctgagtatta gggagatcgt gtccttacta tctaactctg
    29761 agtcacaagg aacaatgggg actgtaacaa aagtcttgtc acagtccctt attccgaaca
    29821 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aaagccatcc
    29881 gaaagtcaga cggacaagtc tgcttttcag atggcgctac aactactctg cttattcttt
    29941 tagctatcca ttcaattatt tttctctcac ttgttctagc tcttctttgg tcatgggtcg
    30001 atattgccct ttattcaaat ctttaggcag ccaaaatttt cccatacgga ttctttttaa
    30061 atataagact tcttttccta ctgctttgac cattcgctta acttggtgaa actttccttc
    30121 atgcaaaatc agacggataa ttgaagtttc tgtctcttga tctacttgat caatttggag
    30181 ttttgccggc aatgtctgct catcgccatc aataatcagt ccttctgcaa ataaacggat
    30241 atcttcttct gtcattatac cgctaacctc agctagatat tctttctcca catgtttttt
    30301 aggtgatagt aactgatggg caaacacccc gtcattcgtt aaaatcaaga ggccctctgt
    30361 gtctttatct aatcggccga ctggaaaaag atcttcccga taatcctggt cttccaataa
    30421 atcgattacg gtttgatcat gattatcctg agtagcagaa atgactcctg caggtttatg
    30481 caaaatgtag taaaaatctt tttgatacgt aatgatttct cctaaataag taaccgcatc
    30541 ttcgaattca tttacttgcg ctttatcgct tttcaccact tgtccattga cttcgatcgt
    30601 tccttttttg attaatcctt tgacttcttt gcggctgcca agtcccatat ctgctaaaaa
    30661 cttatctaaa cgcatgcgtt tcccccattt caaaagagca tcaatctagc tcttctgatt
    30721 cacttatttt atttttaatt tatgtcgtaa acgcgctatt cctggtccta gtagtctttc
    30781 tgccaagcgg attttcaaag ctaaataacc atacactaaa gcccctatac ctgctactac
    30841 aagaatcaac aatagagact ggaatttgct atcttcgttt aaaaaagcac caaaaatcat
    30901 ctttgtgatc cacgaagcca aaagcataat caaggtaaga ataaagatca gcaatgtcct
    30961 gcgccagaca aaacttggtt taaagcgggc tacttcatgg attctcttta agatcaaggt
    31021 acaagacaca gcaaatccga tcattgtcgc caataaaggg ccgtatactt caaataaacg
    31081 aatggctggg tattgaagaa ggagtttcac tagaaaccca ataaccaaat aattaattgc
    31141 cgccttgttt tcaaacattc cttgcagcat gttggaaact agcatatata gtcctaggaa
    31201 gagtcctaca aaacttgctt ggatcagtac attacttcct agagaatccg gtgtataaaa
    31261 taatgtattt agcggatagg caagtaacat aacaccaaat gtcgccggaa acataaaaaa
    31321 tgaaaacaac tgtaaatttc cgcttgtcaa ttttgccaga cctactcgat ctttcaacgt
    31381 aacagcttct gtaatcaacg gtaacccagt agacgcgatc gatgttgcca atgcaataac
    31441 taccatcgtc aacttatctg gattggcact aaaaatcgaa aaaagatcca gtaactgtgc
    31501 attggaatac tcggtggatg cagacatgat cttcataaaa gtgaactgat cgactaattt
    31561 gaagatcgtt acccctgaac ccacaataat aaacggaatc gcttctttaa cggtatctaa
    31621 caacaattcc ttcgttgcaa tatttatttt gttttcgctg tagtgaataa acgccgatgt
    31681 atacgcttga tgtttcttca aaaaatacaa aaggacaaga atacttgcta gcataccgat
    31741 aaatgccgcg aaagttgatt gcgtgaccgc cgtcacatag tcaccatcca tgactttcat
    31801 gatgatgaat gtagccaata gcatataaaa aatacgtgcc acctgttcta caatctgcga
    31861 cagcgcataa ggcatcattt cttgattgcc ttgcaaatac cctcgtatca cactcataca
    31921 aggaaaaacc aatacagcga tactcaaaga acgcatcgtt ggaatcagtt cttctccgcc
    31981 accagaagca tgagctagcc aaggagaggc gatgtacatg aacaaagcaa acaacaggcc
    32041 taacccgccc atcatctgta atgcacggat aaataatcgt cgtgaagtgc catattcatt
    32101 caatgaatta tagcgtgcag tttgttttgc aatcgcagca ggtattcctg ctgtagagat
    32161 cagtagaaac aatgcatata tattgtatcc catattgaat aatccattgg cagccttagc
    32221 attttcgccc atccaagcat accagggaat gatatagaca gcgcctagca atcttgaaat
    32281 gatattactg gcggtcatcc aagcagagcc tcgtgccatt ttttcttgcg gtgttaattg
    32341 atctaatgct ggttttcttt gattatccat cggtcagatt ccaactttct acaaaacatt
    32401 actatcattt taattggtaa aacagaaact ggcaatcgat tttatataac ttttataaga
    32461 acaattgttt ttttgaattc ggtttcgttt ttttggtaaa gtaaagaaat atacagcctt
    32521 ttatggctaa catttatagg agatatcaat gacacaagaa ataaaaaaac tacccgttat
    32581 atttttaggt cttttcttta tcacagttgg attgaattgg ttcttgctcc ctcacgatat
    32641 agcttccgcc ggagtgggcg ccatcggaca tttagttgaa acggcctttt ctatccaacg
    32701 ctcagttact gtttggagca tcaatcttat gatgctgctt ctagccgctg tcctattagg
    32761 aaaatcagta ttttttaaga ctgtagcggg aagtctgctt tttcctgttg ttttagagat
    32821 tgttccaaaa gtggaaatga tctcctcaca tttcttttca ctgatttcag gaagtctttt
    32881 gttcagtctt ggggtttaca cattgtacac agtcggggcc tcaaatggcg gtataacgat
    32941 accgccgatt attttccaca aattttttcg gcttccaatg ccgcaaggac ttttacttac
    33001 caatagtctt atcgtctttt tgaattatta tgtttttgga ttgttggaga cactattcgt
    33061 tctcctatct attacagtca gttcattgtt tatgaaaata ctcatccgct tgtcccctgt
    33121 gtcaagaaaa tttgaataaa gctggatttt gtcatagcgg aaacaaacga tatattaaaa
    33181 agagtgtgct ataataaacc aagaaattga ataaagcgag ggatcaatca atgagcttct
    33241 ctttagaaga catccgtcat ttactattaa aagaaaatct attaaaagaa tttgtttcaa
    33301 caagaggctg gcatcttgat gtaccagaaa aagctgaatt tcttcagtta agttatgatt
    33361 caagaaaagc tgatgcttct actttgtttt tctgtaaagg aatgaatttc aaagaagaat
    33421 atttggattc agcaatcgaa cagggtattc aatattatgt atcagaacaa ccatacgaaa
    33481 attcagcagt aggaatcatc gtaacggata tccgtaaagc aatggcgctt cttgctatgg
    33541 ctttttacga ctatccgcaa aataaattga aagtgatcgg attcactgga acaaaaggta
    33601 aaacaactgc tgcgtacttt acaaaagcaa tcttggatca cacaacaaat aaaaaaacag
    33661 cattgctttc tactatgaat acaacgttag atggcaaaac atatttcaaa tcccatttga
    33721 caacgcctga gtcattagat ttatatcgta tgatgtcaga agcagttgaa aatggtatga
    33781 ctcatctagt gatggaagtt tcttcacaag cttacaaaac acaacgcgta tacggcttga
    33841 ctttagatgt cggaatcttc ttgaatatct cgcctgacca tatcagtccg atcgaacatc
    33901 cgacatttga tgactatttc tattgcaagc gacaattgat cttgaattca aaaacagttg
    33961 ttttaaatca tgaaagtgac tattttgatt tacttaggga aacagccgat ctattccaga
    34021 ttccaacgat cacttatggt cgttcggaag aaagcaatta tcaagtgatc cgttcagata
    34081 aaggtactca cggttttact ttatcttctc atgaagatcg cttagctatc tgcgatactg
    34141 cttatgatat tttgctggct ggaggattca accaagaaaa tgcagccagt gcgatcattg
    34201 ccgcagcact aacaggtgcc tccagtcaag atgcacaaga cggattaaaa gaagctcgtg
    34261 taccaggccg aatggatcaa ttgatccaat ccaatggagc gcatgtctat gtggactatg
    34321 cacataatta tctaagcttg aaaacattgc tgaaatttgc caaaaatgaa catccagatg
    34381 gccgtgtaat tgttgtatta ggcagcccag gaaacaaagc aatctcccgt cgacatgatt
    34441 ttggaaaagt gctatcagaa acagctgaca tcgccttttt gacggcagat gacccagcat
    34501 ttgaagatcc gaaaaaaatc gcggaagaaa tcaatgaagc aattactaat ccagatttga
    34561 tcgttcatta tgaaatggat cgtcctgaag cgattcgacg cgctttagca gaaagcgctc
    34621 cgcaagattc agtcgttatt gcgggaaaag gcgtggatcc ctatcaaaaa atcaatggtg
    34681 tagatgaacc ttatgaaggg gattatgcaa tcgtgaagcg gttgattgaa gaataatggt
    34741 atttatatac tagtggtttc ccatttctaa gaaaaaaagg gactgtgatt ttttgtcaca
    34801 gttccttttt tcttagaaat cattttaact tttcactagc taattctgct gccttttctc
    34861 tttcttcatt taataattta ttatcataga atttgataaa tgggaaccag ataatgaatg
    34921 cgatgattgc acaaatgact gctaacacag cagctttcca atcaccacca ctaccaacaa
    34981 atgctccgat cccgactggt gaaggccacg gcatctgtgc aagcatcggt ttgacgattt
    35041 ccagccgaat ggcaaaataa gcgagtgtag cagaagccat cggagctaag aaaaacggaa
    35101 ccgctaaata gggattgtag acgattggca tcccgaaaat gattggttca ttgatattga
    35161 ataatcctgg tacaagtgag gcttttccta acactttcag ctgatcagat ttcgcgagga
    35221 atgcaataaa gatgatcaat cctaaagtcg cacctgatcc accaacggtt acataagaat
    35281 tgttgaattc tcctgccaac ggataatttg cgccttgtgc gttagctgcc atattagcta
    35341 atacgatcgg ggtcaagaaa gaagtgatga tattcgctcc atggatccct acgatccaca
    35401 atgcatggat caagaaataa atcaccatga caccaagcca tgtattcgtc aactgcgtaa
    35461 caaacccaaa tgggatagca atcattttga agatatccgt tcctagtgca actaataatc
    35521 cattgatgat catcaccaca aacgcaatga caaatgttgg gatcagagca gtgaaagaac
    35581 gagaaactcc tggcggaaca acatctggca tttttatgac ccaatttctc ttgacacaca
    35641 tacaatacaa ttcgaccgca ataatcgaca taataatagc tgtaaagatt cctgaagttc
    35701 ctaaacgttc gacaaaagag cccatcctaa taccatttac tacagtttct gtctcattga
    35761 tagaagcaat caagctgatt ttaccatcag tcatgatcaa ttcagggata cacataaaga
    35821 atgcaaaaac agatagtaat gcccccgtga gcggattaac atttaatttt tgttcatctc
    35881 gttcaattga cgttaattca taagcaaaca caatggcaaa gtaaatagat aaaatcccca
    35941 tcgtcattgt attgacgatc atgtacaagt cactaacttt gaaaaaagtt gcattaaaaa
    36001 atccttctaa aaatgggaac ggtaatggca aaatatttaa aaccaaaaac atcgagccta
    36061 caatcgtgaa aggaatagaa gccatccccg ctgccatgac agcccgaacg attttaagtt
    36121 gcgcaatttt ccccataggt cccatcaggt atttctgcaa aaaatcaaac attagttatt
    36181 ctcctttcct aaatataacg ctttttcata ttgtttaata cggatatact gttggtcttg
    36241 gttgagtgaa attttcttta aacgatactg tataaatgca ctggcaaaaa taccgcttac
    36301 aacaagaatg aaaacaaata actgtcctcc attgttattt gccataaaag gatacaagaa
    36361 tgtaaatcct gaggtaaaca acactggaat cactaataaa ttaaagacac tctggaacaa
    36421 aaaataccac tttgtccatg gaacgttcgt ttggtgtttt cgataaagct ttacttgttc
    36481 tcctactgca ggcaaaatag ctaaaatcag aatcatcggt aaactacttc ctaaactttt
    36541 agctgataga agcagaatca tccattgcat gttcatgaaa aagaaaaagg cagtcaggta
    36601 acgtacgatt agataacgat tgaaatacat gtttttcaaa ctaagttgct ttttttctaa
    36661 ttcgattttc ctttttactg tatcctgcac aatgatcctt cctttctatt tatttttttg
    36721 atacaaataa ttcatttcct gtgcaacttc caataatgtc atcgtcgtca tcagatgatc
    36781 ttgtgcatgg accatgataa tttccatttc tatttttgtc ccgctggcat attctttcag
    36841 aagttgagtt tgtgactgat gcgcatgaag aatctcttca tttgcttgtc gcaacttctc
    36901 ttctacttct tcaaacatct ctttacgcat cgagcgaaat gcttcatgta tcagtgtccg
    36961 tgcatttccg ctatggaaaa tgatatcaaa agaagtcact tgtagtgttt cactgtttat
    37021 tttttcagcc atctgttttc ctccttattc gatcagtttg ataaatttac tgctgaaatt
    37081 ctcaaaaata ggttcagcta agatttcctg ctgtactttt agctggtcaa ctaacctaac
    37141 gattgcttta gtcacgaccg taattccttc attttcgaca taagaaggcg aaactaaaaa
    37201 aacaaaatga atttcttgat attgatccca ctgcatccca gaaggaatca atgcaacggc
    37261 tacctttgtt tcgattccaa ctggaacagc gggatgtggg actgccactg tatcactgaa
    37321 tatgatttgt cccatatttt cacgttgctg gatctgcttt cgcatttctc ttatatatcc
    37381 ttcttcttca tttatgctca gcttttgcag caaatcatga agaatttctt cctttttcgg
    37441 ttcgtacgta taagtcttga aaaatttttc agacaactgt tcggtgtaaa tctgcttttt
    37501 ttgctgtaga gaaaatgctg gtatcggtct atctgaggaa tgatagggta tagattctcc
    37561 aactaccttt cgtattttac gaacatcatc ttcattcaaa aaaatactga catgtaaaac
    37621 tggaattttg aaaaacattg tcgaaagatc aatagaagaa ataatcaggt cgattccttt
    37681 taaagttgtt tcattgatct cataatagcc ttttacactt accactgtaa tatttttacc
    37741 gaattcactg actacgcgat tctttaatag ttgggcactg ccgtaacccg ttgcacagat
    37801 aatcaatgca tgtagtttat gcgcagctct ttctttttcc aatgccgcca tgagatgcaa
    37861 agccaaatag gcccattcat cttcattgat ggtatagttt ttcaaagccg gcatgttgct
    37921 gaaatgcctt tttactgcgt caaagacttt gggattttct ttctttatct cctcagtcaa
    37981 tgggttctca atgcgaatac ctcggctcaa tcgaatcatc atcggtgaca aatgatcgag
    38041 tagtccattg aaaagctgga gatcctctac taatgaaaaa tgaaattcct gagacaatct
    38101 tttcagaact ttcgctaatt cctttgatag ttcctgatca ttttgatttt ctttgtgatt
    38161 cgacttagcc atgagatgta atgtcaaata agcaatttct tcttcgggaa acgatacacc
    38221 tgcgattacc tcaatacgtt gaacgatccg ctgcgccact tgatactcta ctcgcttaga
    38281 aacttcttgt tcgatgccta attccttgat ttcaaaccct tctcggagcc gtttgatccc
    38341 caaagccaag tgtaaagtca gattctgtat gataaaatcc gaaactttta acttagcttc
    38401 tcttgtttca tcgagaatga taatgactag ctcttcaaaa ctgatgtctg ggaaaaaacg
    38461 gctttgccct aaatattctt tcaatgaatt tccataatgg tttccaaaaa aagtatccat
    38521 gataaaatgc cgtcgatttc tttcttcccc ttcgatccat gtcccttgac catgcttaga
    38581 aacgattttt agtgaatatg gaatcaatcg ctccttgatt tcttgcatgt cctttgtcaa
    38641 actggaacga ctgatataaa gctcttctgc caaatcatcc ataaaaagaa tggcatcttc
    38701 caaaagcaat tggttcaata tatacttttg tcgatcttcg gactcataaa atccttgcgg
    38761 ttctctagca tatccaggta ctatttcttg ccgactcaaa aacaagtcaa atgtcagtct
    38821 ttgtaaaata tgcagttggt agccataacc tggtttagca atgatcttac ctccgttttt
    38881 ttctactaac tctttgatcc gctgcaagta attacggatt gtccgatcag ataataacaa
    38941 ttcagaagct aaacgttgac tggtgacata ctccttcttg tgatcaatca aatatctcaa
    39001 cagcaatttt tccttctgtt tcattatcat ccactcctat tccatcatat gttctcttcg
    39061 atcacctctg ctaacttctg gatgcctgta gggattggga catacgcttg gaaagggata
    39121 ctaacaacag gctttccaac agattctcct aattttttga atttatccaa aaacatcgtc
    39181 gtttgcgggc tgatcagaaa taaatcaaaa tcactattct taatcatatt atcgccttct
    39241 gtagctgaaa ctgcatctac gagtacctct tctctttttt gcttaaagaa atccgttgtc
    39301 tttgaggcca tcatcgagga tgacattcct gccgcacaaa taatcaatgc ttttttcata
    39361 aatcaatcac tccttttatt tttataaacc aagcatatct tgtaagcgtt ttctattcaa
    39421 aatatatatt tccgtagcat tgcggaaaaa atgtgtatca tatcttgaat aataatttct
    39481 ggcaccgtga tatactagtg atagaatttt ttcaggaggg gatttagtga gtatcacagt
    39541 gaatattttg tatacaagta caaatggaca agccagaaaa tttgcagaag aaatggtcga
    39601 aagaaacatt gtcaaagcga ttcgtgcaga agaaggaaac gagaaatatg actattactt
    39661 tccactagaa gatccagaat ctcttttact gatcgaccgt tggaaagatg aagaagcaat
    39721 cgaaaagcat cacaaatccg aaatgatggc tcaaataaac gaactccgta aaaagtataa
    39781 attaaaaatg aaagtcgaac gtttcactga tttaccgtaa aaagatacac atggaatgat
    39841 agcaatttat catccatgtg tatcttcact ttttccttat tttttattcg atctcatcta
    39901 atgattcaaa ccgtgttttt ctcttatttt tcggataagt atttcagtca ccccaatata
    39961 catgattcct aaacataccc caaggatatt ctcaaaaatg cggatcgtta ctgctcctgg
    40021 aaccccgaat aaactggcag tgattgccaa agcaccaaaa gaattaaaaa ctgttttata
    40081 tctataggta gaacaaacac ctaaagctag accacctaat ataccgaccc agttaaaagg
    40141 aataaattgg ctgataccaa taaataaaac acatccaatc aatgagccaa tgattcggtc
    40201 tactgctcgt tctttgaaac caatcgacat caatccatag ctagatacaa tagaagaaaa
    40261 tgcaaaccct gcccacatca atcgctgaaa agggacatac tctcctgcaa cgatcaaaag
    40321 actaatgcca aatgcataga tgagcatcca aattttttcc tgcgaataca tgcctttcaa
    40381 aaaaatctct ttaaacaagc ttttgtcttt gttcttttct cgatgttttc tgtaaaggag
    40441 aactgaaaac caacaaaaaa acaaaaataa taagctactt gtttgtgtaa agaaattctt
    40501 gttcaacgaa tctttaggta acgaataaac aataaagaga tatgaaaagc cgtacaaaga
    40561 agcattcccc attttgggat cactagcggt tgccattaat atgctgctta gtaaaataaa
    40621 atgaatcaac atgcccataa ctgaccattt gatcagctgg acatacggtg ctactaatag
    40681 tgagaacatg acgattgcta aacctatgat cgaatgggaa atcttataac caaagtcaac
    40741 aaaacgaata ctcatcaaca tacagaataa aaccacagcg tatggtttat gcttcgattc
    40801 aaataatgtt gtaattataa tgataaaaca aatggcaaac aatacgatga ataatgatcg
    40861 taaaaacaat gaacaaatat acatgcactt ttctttttta gaagctgctt ctcgtatttt
    40921 ttgttttaaa ataaacggat ctagttgtag agactgataa aaatccacct gttgtcgtcc
    40981 tttcttttta cttttatttt ttacagtcat ctatgacagg ttgtaaatac tatggaacat
    41041 catatggcgt tgtcattttc ttgtcaacta tttttcctat tcatgttttt ataaaaaaac
    41101 atatcgtttt cttgtatgat taaatcacca caaaataaac aactgagaga acgatatgct
    41161 ttatgaatgg ttctatcaaa aaaattctga gatcaataga caaaaatttg atgattacag
    41221 aggtctctta cgaaacctta cagaagaaaa aaacattgat cgttgctgca gttctcttcc
    41281 ctgctccatg tgtctgtaga aattgtggtt ctgctgtggt agatggaaat aggaaattaa
    41341 tggtggtgaa aaacaagaag aaggaaacca ttgtccactt tgaataatat aatcatatgc
    41401 caatggttat acgcctaaaa aagcagcgct atacctgtaa aaactgtcga agccattgga
    41461 acgctcaaag ttattttatc cgtccaaggc actcgatttc aaatcatgtc agacataaaa
    41521 tcacttcttt actgactgaa aaagtgtcgt tattttttat ttcgaaaagt tgttaggtat
    41581 ctttgacaac cgttattcgc acattgaaag agttgagaag ctatttacca aagtcttcca
    41641 aaagaattct gtcttgagtg ttgagggttg tgaatttcgt tctcacgttt ccgttgagga
    41701 taaactgagt tttatttccg cggatggcga gacagaaaaa ttaatcgaga aaatttacct
    41761 acacaaaaaa ttcctcgatt aacaaactat ttccttagct gcactaatcc taaagaaata
    41821 gaattcttag ttacagacat gaacgccacc tactttcagc tcaccaaaca tgttctacca
    41881 aatgcgaaat tggtgattga tcgattactt ggtttttcca cgcctttcaa ggaggcgtaa
    41941 cctttattca cgagttggtt gaagctttcc gagacaaaag acccgacttg tttttctcct
    42001 tattgacaaa gagccattaa aactagagaa aaaattacaa ctgatcgctg attatttgta
    42061 gaaagaaaat caaagtatta cccaataaac ttgagtcatt aggcaaaaaa cactgcgaaa
    42121 ctcaagaatg attttcgcag tgtttttgta tattttttat taactagtat tactacaaag
    42181 gtttattttt cacttctcaa cgcagcacaa aattgtcttt acttatttac tggttgggtt
    42241 caaccttctc tactccgata ctaatatttg tttgtatacc ccttctaaaa tcggtagttt
    42301 ccattcaatt gatgggtact agttgatttt aaaaactatg cttattcaat tttttatttt
    42361 agctcatata tttcgtttct ttctaaattt tatactgatg aaaacaagta gcacacccaa
    42421 tattgacaat ataaactggc taatgatggt ttctccagtt tttggaagat gcttatttcc
    42481 actgcttgtt ggattgtttc ctcttatatc tgagctatca gcaacttttc tattctcttt
    42541 tgttaaaaag atcgccactt cttgttcttt ttcgatcttg aaagttactg gtgtcgcatc
    42601 caattcatac cccgttggcg cttgagtttc taccagctga tatgctcctg gtgctaagcc
    42661 atctatcgcc agttttccat ctgctcctgt ggttagacct gactgcagaa cggttccatt
    42721 ggcatcttgt aactcaaaaa cagctccttg caggacttct ccgctttggt catcgatctt
    42781 tgtcaacacg acaccgcctg gtgtaagtcg attttctttc gtcaattcta ccaccgctgt
    42841 ctgacttctt tcgatctcaa actcgattgg tgtcgcatcc aattcatacc ccgttggcgc
    42901 ttgggtttct accagctgat acactcctgg tgctaagccg tcaatcgcca gttttccatc
    42961 tgctcctgtg gttagacctg actgcagaac ggttccattg gcatcttgta actcaaaaac
    43021 agctccttgc aggacttccc cgctttggtc atcgatcttt gtcaacacga caccgcctgg
    43081 tgtaagtcgg tttgctttcg tcaattctac caccgctgtc tgacttcttt cgatctcaaa
    43141 ctcgatcggt gtcgcatcta attcataccc cg
//
LOCUS       DADXTG010000009        42129 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.009, whole genome shotgun sequence.
ACCESSION   DADXTG010000009 DADXTG010000000
VERSION     DADXTG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.009"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..281
                     /locus_tag="IWI57_000376"
     CDS             27..281
                     /locus_tag="IWI57_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2448546.1"
                     /translation="MQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILN
                     VYQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            460..690
                     /locus_tag="IWI57_000377"
     CDS             460..690
                     /locus_tag="IWI57_000377"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBC2448547.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            724..906
                     /locus_tag="IWI57_000378"
     CDS             724..906
                     /locus_tag="IWI57_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HBC2448548.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            1019..2530
                     /locus_tag="IWI57_000379"
     CDS             1019..2530
                     /locus_tag="IWI57_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448549.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMGV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            2853..3077
                     /locus_tag="IWI57_000380"
     CDS             2853..3077
                     /locus_tag="IWI57_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBC2448550.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            3070..3636
                     /locus_tag="IWI57_000381"
     CDS             3070..3636
                     /locus_tag="IWI57_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBC2448551.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            3624..3854
                     /locus_tag="IWI57_000382"
     CDS             3624..3854
                     /locus_tag="IWI57_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448552.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            3863..4420
                     /locus_tag="IWI57_000383"
     CDS             3863..4420
                     /locus_tag="IWI57_000383"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HBC2448553.1"
                     /translation="MSLNLYDNGDKDFHSKEQQLNISKTLRSAFPNHRTTTSVFYTQE
                     SAFMNKPVLFVYRKKKEYKKMSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNIL
                     EQRKKLVERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQA
                     HIAALHKDQQQIAEAVKKTEKGDDS"
     gene            4420..6147
                     /locus_tag="IWI57_000384"
     CDS             4420..6147
                     /locus_tag="IWI57_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBC2448554.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            6177..8093
                     /locus_tag="IWI57_000385"
     CDS             6177..8093
                     /locus_tag="IWI57_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2448555.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            8778..8960
                     /locus_tag="IWI57_000386"
     CDS             8778..8960
                     /locus_tag="IWI57_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448556.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            8987..10684
                     /locus_tag="IWI57_000387"
     CDS             8987..10684
                     /locus_tag="IWI57_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2448557.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            10709..10840
                     /locus_tag="IWI57_000388"
     CDS             10709..10840
                     /locus_tag="IWI57_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448558.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            10932..11795
                     /locus_tag="IWI57_000389"
     CDS             10932..11795
                     /locus_tag="IWI57_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448559.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            11832..12368
                     /locus_tag="IWI57_000390"
     CDS             11832..12368
                     /locus_tag="IWI57_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBC2448560.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            12382..12804
                     /locus_tag="IWI57_000391"
     CDS             12382..12804
                     /locus_tag="IWI57_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBC2448561.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            12734..15133
                     /locus_tag="IWI57_000392"
     CDS             12734..15133
                     /locus_tag="IWI57_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBC2448562.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            15165..17156
                     /locus_tag="IWI57_000393"
     CDS             15165..17156
                     /locus_tag="IWI57_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBC2448563.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            17179..17430
                     /locus_tag="IWI57_000394"
     CDS             17179..17430
                     /locus_tag="IWI57_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBC2448564.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            17420..18649
                     /locus_tag="IWI57_000395"
     CDS             17420..18649
                     /locus_tag="IWI57_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBC2448565.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            18646..20727
                     /locus_tag="IWI57_000396"
     CDS             18646..20727
                     /locus_tag="IWI57_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBC2448566.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            20876..24796
                     /locus_tag="IWI57_000397"
     CDS             20876..24796
                     /locus_tag="IWI57_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBC2448567.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            24797..25741
                     /locus_tag="IWI57_000398"
     CDS             24797..25741
                     /locus_tag="IWI57_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448568.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            25745..25948
                     /locus_tag="IWI57_000399"
     CDS             25745..25948
                     /locus_tag="IWI57_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBC2448569.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(26248..26484)
                     /locus_tag="IWI57_000400"
     CDS             complement(26248..26484)
                     /locus_tag="IWI57_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2448570.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(26667..27053)
                     /locus_tag="IWI57_000401"
     CDS             complement(26667..27053)
                     /locus_tag="IWI57_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBC2448571.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(27035..28363)
                     /locus_tag="IWI57_000402"
     CDS             complement(27035..28363)
                     /locus_tag="IWI57_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBC2448572.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(28324..28653)
                     /gene="mobC"
                     /locus_tag="IWI57_000403"
     CDS             complement(28324..28653)
                     /gene="mobC"
                     /locus_tag="IWI57_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBC2448573.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(28911..29282)
                     /locus_tag="IWI57_000404"
     CDS             complement(28911..29282)
                     /locus_tag="IWI57_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2448574.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            29427..29612
                     /locus_tag="IWI57_000405"
     CDS             29427..29612
                     /locus_tag="IWI57_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBC2448575.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            29770..29937
                     /locus_tag="IWI57_000406"
     CDS             29770..29937
                     /locus_tag="IWI57_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448576.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            30124..30342
                     /locus_tag="IWI57_000407"
     CDS             30124..30342
                     /locus_tag="IWI57_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448577.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            30555..31214
                     /gene="vanR"
                     /locus_tag="IWI57_000408"
     CDS             30555..31214
                     /gene="vanR"
                     /locus_tag="IWI57_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBC2448578.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            31217..32560
                     /gene="vanS"
                     /locus_tag="IWI57_000409"
     CDS             31217..32560
                     /gene="vanS"
                     /locus_tag="IWI57_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBC2448579.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            32731..33537
                     /gene="vanY"
                     /locus_tag="IWI57_000410"
     CDS             32731..33537
                     /gene="vanY"
                     /locus_tag="IWI57_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBC2448580.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            33555..34382
                     /gene="vanW"
                     /locus_tag="IWI57_000411"
     CDS             33555..34382
                     /gene="vanW"
                     /locus_tag="IWI57_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HBC2448581.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            34379..35350
                     /locus_tag="IWI57_000412"
     CDS             34379..35350
                     /locus_tag="IWI57_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBC2448582.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            35343..36371
                     /gene="vanB"
                     /locus_tag="IWI57_000413"
     CDS             35343..36371
                     /gene="vanB"
                     /locus_tag="IWI57_000413"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBC2448583.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            36377..36985
                     /locus_tag="IWI57_000414"
     CDS             36377..36985
                     /locus_tag="IWI57_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBC2448584.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(37051..37224)
                     /locus_tag="IWI57_000415"
     CDS             complement(37051..37224)
                     /locus_tag="IWI57_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448585.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            37572..38003
                     /locus_tag="IWI57_000416"
     CDS             37572..38003
                     /locus_tag="IWI57_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2448586.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            38010..38243
                     /locus_tag="IWI57_000417"
     CDS             38010..38243
                     /locus_tag="IWI57_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2448587.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            38667..38867
                     /locus_tag="IWI57_000418"
     CDS             38667..38867
                     /locus_tag="IWI57_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBC2448588.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            38951..40144
                     /locus_tag="IWI57_000419"
     CDS             38951..40144
                     /locus_tag="IWI57_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2448589.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            40322..40552
                     /locus_tag="IWI57_000420"
                     /pseudo
     CDS             40322..40552
                     /locus_tag="IWI57_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            40680..41363
                     /locus_tag="IWI57_000421"
     CDS             40680..41363
                     /locus_tag="IWI57_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBC2448590.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            41672..41745
                     /locus_tag="IWI57_000422"
     tRNA            41672..41745
                     /locus_tag="IWI57_000422"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41706..41708,aa:Arg,seq:tct)
     gene            41904..>42129
                     /locus_tag="IWI57_000423"
     CDS             41904..>42129
                     /locus_tag="IWI57_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBC2448591.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSA"
BASE COUNT        11671 a         9688 c        11192 g         9578 t
ORIGIN      
        1 tctatgttta cgaggataaa caggaaatac agggttttat agggttaagt aacgaataca
       61 ttgaaggtat ttttgtttct gccgaaatgc aatctcaagg tataggtaaa attttgctaa
      121 actatgtaaa gggaaaaaga aacaaattaa tcttaaatgt atatcaaaaa aatacacggg
      181 caatatcctt ttatcaaaga gaaggatttg agattcagta tagcggtttg gatgaagcta
      241 ctggagaaaa agactatgta atggcatggc agcagaaata gaaattcaag ttttagtgtt
      301 tggaaaatta tgacttagtg tgcaagttgg gagaaaatat ggtaacggaa aaagagaaaa
      361 aagcatgtcg atttattgcc gaatggctat aagattgtga gacagtctct ggtagatgat
      421 ctggagggcg aaaaaaaggt atgttgggag acagaaattt tgcaggatga tgatagtgga
      481 tttacttatg aaatcagcag gtgtctgtat ttcgatacct gtaaggaaca tggctatgca
      541 gagttctgca aagtattttg caaccatgac tggtatgctt atggagtatt aaagcatcat
      601 tcgagattta tcagaaaatc gacgattgcc gaagatggaa ctgtatgtca tgatacaatc
      661 gagaaagtaa cagagaaaca ttttttataa aaccgaaaaa ttgattatgg gaggaaattg
      721 aaaatggcga aagaagagag atttgaaaaa atatattctc agggagcatt aactactact
      781 gagatatggg tggataagga aactggtgtg aattatttat atcatgcatg tgggtattca
      841 ggtggactga ctccattgtt ggacagagat gggaaaccag ttatttctca ggctataaat
      901 agatgacctt agaaacagca actgttatgg taagtaacag catcgtcaca atatgtatat
      961 ggtcgattgg tgtgtgcggt aatatactat attcagaaaa acaattcagg agatttttat
     1021 gagaagatca ggttggacaa aatggttgat cgtgattccg gcaatgctag gcattgtgtt
     1081 ggtgacctat gtgcggtata caacggatat ctggggattt ggtgcattta tctgccagct
     1141 gattgcaatt ttggaattgg cttatggtct gcgaattgcg atgctggcac agaatagaaa
     1201 gaagtcctat cggttaacac cggaagaacg acatgagtat gcacggtatt tatatgagaa
     1261 gcaatatcac cgttaccctg ctgtagcgaa tcagatgctt ttggttatgg cacggatggg
     1321 tgtactcctt aacaattatg aacgggctgc acaggagctg gcagatattc gtatagataa
     1381 attcaatcca gcacagttga agttgtatta ttacctgaaa gtggttacag caatggcagc
     1441 aggagatgcg acagggattc aagagagcca gatgtgttac gcagggattc cggatactaa
     1501 atgcacatat ccgtcaaaag cagagcttgc tatgtggata gagcagagag cggcagcgca
     1561 gatggcagag gcactgaaga aggcagtgcc ggataaaaaa gagcatccga tacggatcag
     1621 tatcattact ttggttcttg cgtatagtac cttcttctat ggattgtggt atggtataaa
     1681 acgtgatatg ggatatgaag tgagatatcg tttcgcagaa atatcgatta ctgttattac
     1741 aatcagtctg gcggttcttt tgatctgggg gatcgtgagt ttatacagac acaattgcca
     1801 ggcattatcc atacaattcg gtaaaaacaa agtggcattg ataattgtct atggagctgc
     1861 tgcgatattt gcacttttga ttattggaat gagtggtctg agtgtagtgt tcggaatgga
     1921 tggaacagaa acggtgactg gttgtgacag caaatatacg tatataactg tgcaggaaga
     1981 ttatggtggt gaacggagat acagaaccaa taatccattt gttatgcaga gtatggatgg
     2041 tctgtggtgg tcttcggata caatagatga agaagataat caagaagagg atactacaga
     2101 attaccggat ttggatacag agaattcaac agattcagag gatattcaga gtggaaatac
     2161 catagaagat tacgagacag aagggcagca tgatggtctg acaattcaaa atgcaatgct
     2221 ggcagtttat aattaccttc aggagcagaa tacacttcaa aatatggcgt tcagttacac
     2281 ggcgaatgca aagggagaag tctatgctgt cgtcagcgaa acgcaggaaa caaagaacgg
     2341 taatacggta aatgtgagat actgtctgta cgataatggt tccaagacag atgcagatgg
     2401 taattcctgc gaagaactgg tgttagaaaa agtatatccg gatggcaatt acgagaccga
     2461 attggtagat ttttatctgg taaatgcaga tacgatgcag gtgacagacg aacagaagaa
     2521 tacgtggtaa aataaattct ggggaagtag gtgcattgta tgacaatgag agaaaaaata
     2581 aaactattat cgtaaatgtt tcaagcggac tgggttcttt tggaagtatg caaaaggact
     2641 ccaagacata agaggtgaac tgcgctgatc caggtgccac ggcaacagat ttgaatgtcg
     2701 acggaggaaa gcaaacagta caggcgcaat cgtaaaactt gcaatgattg ataaaaccgg
     2761 actaacagta acttttatta gtcgatttgg aaatatgcct tggtaatcaa caattccggt
     2821 tttactgact aagaaaatga ggaggattca taatgagtga gaaaacagag attactttta
     2881 tgcaaacaag actaatccgg cttgcttcag aagagtggca tttacctgtt gaacagatta
     2941 tccacttgtt taaagaagcc gatgttcttg gatatataga aaaatgctat gggatttttc
     3001 attgtgaagg cgatgaagct gtgcttgaag atattactga atttttgcaa agaaagggga
     3061 tagagattag tgcttgagtt aaaagatgga tttgttctct atcatggaag ttattgtgaa
     3121 gtaaaagagc cagatcttgc caaatgtgca aagagaaaag attttggaca gggattttat
     3181 ttaaccacat caaaggaaca ggcagaaagt tttctgagaa cctcaatagc caaagccatc
     3241 gcaaccggaa caattgaaga ggggcagaag tttggatata tttcaacttt tgaattcaaa
     3301 ctttcagaaa atcttgaaac gcatattttt gaaaatgcag atgtggactg gttacattgt
     3361 atcgccgctc accgaaagaa aaaaatgttt attgaggttg aacgtgaaat ggcaaaatat
     3421 gatatcattg caggaaagat tgcggatgat gcaacaaacg ctactcttac cgcttatctt
     3481 gcgggtgctt ttgggactgc aggtgataaa gtagcagacg atttctgtat cagacagttg
     3541 ctgccgaata aattgaaaga tcaatactgc tttaaaactg aggcagctat tgagtgtctg
     3601 aaatttgtga aaggtgagaa gatatggctg aagtaacaat tccaaaggaa aaaatgaatt
     3661 atacaatcga ccttctgatc actatggtga cagatgaaat cgcagaagag acaggcaagg
     3721 acagaaacga ggtattgaca gattttttgt gttcaaagac aggaaaagca ctgtatgatg
     3781 agaagacaaa gctgtggtgc actggtccgg catatatagc tgaactgtat atggaagaat
     3841 taaagaaatc ctgatatggg atgtgagttt aaatttatac gacaatggtg acaaagactt
     3901 tcattccaag gagcaacaat taaatatttc aaaaacgctg aggtcagcat ttcctaatca
     3961 caggacaacg acctcagttt tttataccca ggaatcggca ttcatgaata agccggttct
     4021 ttttgtttac agaaaaaaga aggagtacaa gaaaatgagc agagcaaaaa atccaaagta
     4081 tgaatccatg tcattggagg aactgaaggc caacatggaa aagagcagac aggaactgga
     4141 ggcagcaatt caccacaaaa acattctgga gcagcggaag aagctggtag aacgcaggga
     4201 acgttcccac cggctgattg tcaaaggggc agagtttgag aaagtatttc cgctgtccag
     4261 agatctggaa caggaagacg tccaggacat gatgaatcaa ctgcagaaca gcagttacaa
     4321 caagagtatt gtgcggcagg ctcatatagc cgccttacac aaagatcaac agcagattgc
     4381 agaagcagta aaaaagacag agaagggaga tgacagttaa tggcactgta tcatttttca
     4441 gtaaggaatg tatccagagg gaaagggcag atggtggtcg cttccgcagc ctatatttcc
     4501 gggcagagga tttttgacag ttattacaac aagatacacg attatacgag caagtcaggt
     4561 gtgatattta cagaaatcct gacaccggag tatgtgccag agcgtctgac tgacagggaa
     4621 acactctgga acgaggtgga gcatgtggaa cggaacaaca aggcacagct tgcctattcg
     4681 tttgacattg cactgcagaa tgaattgaca cttgaggaaa atatcagact ggcaaaagag
     4741 ttctgccagg agcagtttgt ggcacgtggc atgatcgtgg atctggcagt ccatgaggga
     4801 aaagcggaaa atgaagaaga accggataat ccccatttcc atgtgcttgt cccaatccgt
     4861 ccttttacag aggaaggaat ctggggcaat aagcagagaa gggaatatat tcttgatgag
     4921 gatggaaacc gggtgaagga tgccaaagga aaagatatgt tcaatgctgt ttctactacc
     4981 ggatggaatg atccggagct tttaaaagaa tggcggcggg catggacaga gaaagtaaat
     5041 gggaaattca gggagtgcca catggcatcc agaatcgacc accgttccta caaagaacag
     5101 gggattgacc tgatcccgac catccatgag ggatatgaag tccgggcaat ggagaaaaag
     5161 ggaatcaaaa cggtgatcgg agaattgaac cgggcaatcc ggcagtttaa ccagatgttt
     5221 atttctctga aagaatccat tcagtggatg aagacagctt atgaggaaat gaaagcagag
     5281 ctggatcgca ggcagaaccc gacactgctg gaaagcctgc aggattatta tgataaaaag
     5341 acacagggca gaccaccact tcccaatttt tatgcggaga tgaaacagag aggtaagaat
     5401 ctctccaatt tacaggagtt tgcaaaatcc atcagttatc ttcagacaca tcagatagaa
     5461 acgatggatg acttgcagga acggattgag gaactgaacg gtgttgtttc tgtcagtaaa
     5521 aaagaaattt ctgaaaagcg gaaacagtta aaggaacttg agaatctgga aaagatggca
     5581 gaggtaataa aaacaaatca gccattgatt gatgagtata accatttcta ttttcagaaa
     5641 agacgggaga gatattatca gcagcataaa aaagaaatca attattaccg caaatgtgaa
     5701 cgggaactga aacaacatct ggataaaaat ggaaaagtgc caactgccag atggaaacgg
     5761 gagaaagaag aacttcaggc ggggatcgaa gagctgaaag cagataatca gccgtaccag
     5821 gaggaactaa cttttgtgaa aaaggtacag agctgtgcgg atattgccag acgtgaccgg
     5881 gaaatggcag aagcagatac ttccgggcgt tcagaagaaa aaagggagaa gcaggtgaag
     5941 ttcccgacat tttacacagt tcaggcgaaa gagattcttg aagaaaacgg gaaagcagag
     6001 caacagccgt tgaaccagtc ggaacagaat ccagaaaaga agacctctct tctgagaaaa
     6061 ctggatgaaa aaaagaaaga gtgtgcagag cgtgatgcaa aacaacaggc agtgaagaaa
     6121 aagcgaaact atgacatgag tttataacag tctgtcagca ggacagacgg gaaaggatga
     6181 gcaaaatgca gtatacagaa gaacaaatta taagagcaaa tcagacagat cttgtttctt
     6241 ttctgagcgc acagggagaa caactaggga aaagcggaaa ggaataccgt tggaaaaagc
     6301 atgacagtgt aacggtatcc ggaaaccggt ggtacaggca cagtcagggg aggggtggct
     6361 atccggtgga ttttgtgatg gaattttaca atgccacatt tccggaagct gtcaagatgc
     6421 taaccgggga ggaaggcgaa ggcaggaaca gtacctgccc cgccccgtcc cctgacttcc
     6481 gattaccaga gaaagaggaa aataatgata gaatcatcag atatctgaca gaaaacagag
     6541 ggattgaaaa gaatatggtg gaggaatgga tcggcagtgg ggatatttat gaagaaaaga
     6601 agcaccataa tgtggtgttt gttggcagag atgcagacgg tattccaaga tatgcccatt
     6661 gcaggggaac tggtgaaaca aaataccggg gagatgtggc tgaatctgat aaatcttacg
     6721 gattctgtca cagagggacg gataatcagt tgtttgtgtt tgaagcagcc attgatctgc
     6781 tttcttttat ccaattattt cccaaagact ggaaaaagcg gagctacctg agtctgggtg
     6841 gcatttccag tgcggcactg atggcttttc tttctgaacg ccctcagatt acttcggttt
     6901 tcctgtgcct ggataatgac catgcaggga atgaagccag tgagaaattg gcaatagaaa
     6961 tcccggacgg atacagtgtc atccgtctga aaccgtcccg aaaggactgg aatgaaatcc
     7021 tctgtgataa aaatgcagac aggaagaaat caatcattga aacagttaca atgaaagtac
     7081 cggagaagga agaactggtg ccgatgttgt gttatgaaga cattgaacag accagtgtag
     7141 aatggctgtg gtttccctat ctgccatttg gaaaactgac gattattcag ggcaatccag
     7201 gagagggaaa aacttatttt gccatgatgc tgaccgcagc ctgcacgaac cgtaagactt
     7261 tcccgaacat ggaagagatt gaacctttta atgtgatcta tcagactgca gaagacggta
     7321 tgggagatac gattaagccg aggctggtgg aagcaggagc agacctgagt cgggtgatgg
     7381 tcattgatga tacagaggaa gcattgacgt tatccgatga ccgtattgaa aaagcaatcc
     7441 gtcagaatca ggtcagactt ttaatcattg atccggtaca ggcttttatc ggtgccgatg
     7501 tggatatgaa ccgggcaaat gaagtccgtc cggtattccg gaaactggga atgatcgctg
     7561 aaaagaccgg ttgtgcgatt gtgctgatcg gacatctgaa taaatcttca gggacacaga
     7621 gtacttaccg gggacttggt tctattgata tcatggcggc ggtgagaagc ctgcttttta
     7681 tcgggaaagt aaagaaagac ccgacaacaa gagtactgat ccatgagaaa agttctcttg
     7741 cacctccagg tgagacgatg gcatttaaac ttggagatga agaaggattc cgctgggtgg
     7801 gagcttatga gatttccgca gatgatcttc tggacggaaa ggaaggaaag ccgacagaaa
     7861 caaaactgca acggggaacg aaactgatct atgaactgct tgcagacgga aatgcagtca
     7921 caatccggga actggatgaa aaggcaaaag cacagggaat ctcccagaga accatgcgag
     7981 aagccagaag ccggatgaaa gaagaactgg attatcggat gaatgaaaag caggaaaata
     8041 ccattcgatt aaaaaaacaa gggagaatgg gtgacggcag aattttggag tgaacggcag
     8101 gctttataga taccctatct gccgtctgcc gtctgccggt gcaaggcaat caaacggaaa
     8161 tagcaggtat ccatacagaa taaggattgc tgtgaatctg gaacaaaaat gaattgtaat
     8221 atggcggata taatggtatg attatccggt aacagcaatg ttacggggaa gcacctgtga
     8281 cagggtgtgg ctgcctcctg atatttacag accggagcct ttcggggttc tgagatgaaa
     8341 gaaaggaggt gcgtgatatg acagcttatg agattatctc aatattctta ggcgtactca
     8401 gtttactgat cgcttttggt agcctgttaa ttgcgattat tgcctttctt gatcggaaga
     8461 acaagaggaa ataaaaatgc ctatcctgtc tgccaacagg ataggcggtg gtcgtaagac
     8521 catttagtca actcaggcgg catccacttt ggagtgggtg cttttcctat aacaaatgta
     8581 ccacacattc ctcttgtttt caatgggaaa attaaaatat gcagttcatc tgacagggat
     8641 ttatccctgt ctttattata tccccaattt ctgaaatgtt aatacaaaaa ttttaatata
     8701 tgtaaccaac agtctgggca gttaatctgt aaaaatggat tagctgctct ttttttgcaa
     8761 aaaatggagg ttttacaatg gcagtgaaga agaaaattat caacatcaga gtatattttg
     8821 acggtgatca ggacgcaaaa gaagtgtttg cagaggtgat cgcacagaaa atacgtgaca
     8881 cgaaaacaaa agataaactt gcaataatga aagataagca gtataatgat tatgagttca
     8941 gtaatctgaa atctgcgtag gatgtgcaaa gtaacggagg tatcagatga acaacagata
     9001 tatgagtaac aatatagaaa gaaatgcaac ctggagagtt ggtgtatact gccgactttc
     9061 caaagatgac gaattgaccg gagagagtgc cagcatctcc aaccagagag acatactggt
     9121 gaattactgt cagtcacagg gctggcaggt ggtggatgtg tttcaggacg atggttatac
     9181 aggattgaac acagaccgac ctgatttgca gagacttctc aaagcctgtg aaaaaggact
     9241 ggtgaatctt gtaattacca aagaccagag ccgactggga cgaaaccatg tacagacagg
     9301 atttctgatg gaggagtttt tcccaaagca tggagtaagg tatatcgcat tatatgataa
     9361 tgtggatacc tttgccggag acaatgagat tgccccattt aagaacgtgc tgaacgagat
     9421 gtattccaga gatatttcca agaaagtcca tgcgtcctat cacctgcagg caaccaaggg
     9481 aaagtttaca ggtgttgttc caccactggg atatcagaaa gacccggagg aaaagggaca
     9541 tctcctgatt gacgacgaga cagcaccgat tgtccggaaa attttccagt gggcggtgga
     9601 cggtcatggg attaacttta tatccagaga actggaacgc cagcagattc cctgcccgtc
     9661 atggtggcac cggaaacggg gactccgcac ctattacaca aaatgggaaa agaaagaccc
     9721 ggaaaatggg aaatatgtat gggatgaatc ctatctgaaa tccctgctga tgaatcccgt
     9781 ttactgtggt gacatggctt cccagaaaag gtactaccgt ttcaagattg gcaatcaggg
     9841 agataaagct ccggaggact ggatcacagt cagggatacc cacgaagcaa tcattccgca
     9901 ggatgtattt gatctggtac aggcaaagat gaaaggcagg aaacgccaga cagaacaggg
     9961 agaatacagc atgtttgccg gattactccg ctgtgcagag tgtggcggtg cgttgacgtt
    10021 gaagaagacg cacaccaaag accagcatga agtttatacc tgttccactt atatccataa
    10081 ggggaaagca cattgtaccc agcaccgggt ggacgctgat gacctgtatg atgcagtcct
    10141 taccagaatc caggaatgtg caaaagcagt taccggagac ggaacagaac tggaaaaccg
    10201 ggtaaaggag ttatgcgagg aagatacaca gggacacagg gaaagtctgg agaagctggt
    10261 atcaaagcag aaagaccgcc tggaaacgtt agaccggctg attgcaaaac tgtatgatga
    10321 ccttatcaat gacaagatta cagaatctgt ttttgataag atgctggaga agacacagaa
    10381 ggagcagaca gatatcaaaa aggaactttt ccagaatgag agtgtgctga atacggaaga
    10441 aaaactggat gcacagtccc agcagtggat tgacgatatt agcgagtatg cggacatcaa
    10501 agaactggat gccaatcttc tgaaccggct gatcagtaaa atcgttatct cagaaccgca
    10561 ggagaaagac ggaacagaaa attactgccg tactcctgaa acggtcacaa tggaaattca
    10621 tttcaactta aaaccaatac cggagcttgg aaccatcgaa agaggttctg gctcccataa
    10681 ataagaaaaa tctatcaaaa tttaatctat tttaatcttt tcaaggctca tttcgagcaa
    10741 atattttctc ataaaagaca taaaacagct tgtcgctggc ggcggcgtgg ccctgatcgg
    10801 tgtcactctg gttcccctgc tgtccgggct gctgggctga tcctggcctt tgtccctgtc
    10861 caaaccctta actaatcgcc tccgcgtcct cccggccaga ccgggagggc gcgggaaagg
    10921 aggtgctacc cgtgattagt gaaatcattg aagaatggat aaaaggcatc ctcatggacg
    10981 gtatcacggg caacctgtcc gggctgttcg atacggtaaa tgccaaagtc ggagaaatcg
    11041 ccgccgacgt gggacgaacc ccgcaggcat ggaacagcgg tattttcaat atgctgcaca
    11101 gcctgtcgga aaccgtgatc gtccccatag ccggggccat actcgccctt gtcatgtgct
    11161 atgagcttat ccagatgata acggaaaaga acaatatgca cgactttgac agctccatgt
    11221 tcttccgctg gatattcaaa tccgcgtttg caatcctgat cgtaacgaac acatggaaca
    11281 tcgtcatggg cgtattcgac gccacccaaa gcgtcgtcaa tcaaagcgcc ggtgtgatta
    11341 tcgggcagac atccattgat tttaacaccc tgataccaga ccttgaaagc caattggagg
    11401 ctatgagcgt gggcgctctt ttggggctgt ggttccaaac ccttgttgtg ggcctcacca
    11461 tgaatatcct ttccatctgc attttccttg tgacctatgg ccgcatggtg gaaatctatg
    11521 ttgtcacgtc gctggggcct atcccccttg ccacaatggg caacgcggaa tggcgcggca
    11581 tgggccaagg gtatctgaaa tccctgctgg cgctgggctt ccaagccttt ttaatcatgg
    11641 ttgtggtggg tatctacgcg gtgctgatac agaatatcgg ttccgcttcc gacatatccg
    11701 gcgcaatctg gggctgtatg ggctatacgg tgctgctgtg cttctgcctg ttcaagaccg
    11761 gcagtatatc aaaggccgta ttcggggcgc attgatccca aaatccaagc atacacagga
    11821 ggaacttgtt tatttcaacg tacaacatca tttacgctga cccgccgtgg cggtatgact
    11881 gcaaaaacgt acagggcgca gcggaaaatc actattccac catgagcatt gacgagctgt
    11941 gcgccctgcc ggtggaacgg ctggcgtcca aagactgcct gctgtttttg tgggccacat
    12001 tccccatgct gccggaagct ctgcggttaa tcaaagcgtg gggcttttca ttcaagaccg
    12061 tggcttttgt atggctgaaa cagaaccgta aaagccttac atggttttac gggctgggcc
    12121 gctggacaag gggaaacgcg gaaatctgcc tgctggcaac acacggcaag ccgaagcgtc
    12181 gttctgcctc tgtccatcag tttattatca gcccgattga acaacacagc aaaaagccgg
    12241 atgtgacccg tgaaaaaatc gtagagctgg cgggcgattt gccccgcgtg gagctgtttg
    12301 ccagacagaa aacccccggc tgggacgtgt gggggaacga ggtggaatgt gaccttatcc
    12361 tgacgtgaaa ggaggtgcca aatggcttat gtaaccgtcc ccaaggactt aacccatgtg
    12421 aaaagcaagg ttgtgtttgg gcttaccaaa cggcaactga tctgtttttc cggggcgctg
    12481 gtgatcggcg tccccttata ttttctcacc cgtgactatc ttaccaacag cgcggcggcc
    12541 ctgctcatgg tatttgccat gctgccgggc tttttgcttg ccatgtacga gcgtcacggc
    12601 cagccccttg aagtggtgat tatgcagatc atccagtgct gcttcatcca gccaaaggag
    12661 cgtccctatc agaccgacaa cacctacacc gcccttgtgc ggcaatacca gatggaacag
    12721 gaggtaaagg ccattgtcca aaaaggaaat cccaagagaa accgaaaaaa caaagctgac
    12781 ccgcgcccag cgaaaagaaa ttgacgcggt aatccgtaaa tataagggcg acggcaggcc
    12841 ccacacggcg cagcagagca tcccctacga ggtcatgtac ccggacggcg tgtgccgggt
    12901 atcgcccggc gtcttttcca agtgcattga gtttgcggac atcagctatc agcttgccca
    12961 gccggacacg cagacggcta tctttgaaaa actgtgcgac ctgtataact atgtggacgc
    13021 ctccattcat atccagtttt ctttcctcaa tcggaaagtt gacccggtgc agtacgccaa
    13081 gagctttgag atcgcgccgc agggggacga cttcgacgac atccgggccg agtacaccgg
    13141 cattctgcaa aagcagcttg caaacggcaa caacggcatg gtaaagacca aatacctgac
    13201 attcaccatc gaggccgaaa gcgtgaaagc ggcgcgagcc agattgaagc gcatcggctt
    13261 tgacctgtta ggctatttca agagcatggg ggccgtagcc catgtgatgg acggctggga
    13321 acggctgaac ctgctgcatg gcgtgtacca cccggacggc gagatattca attttgactg
    13381 gaagtggctg gccccgtccg gcctctccac caaggatttt attgccccgt cctccctgtg
    13441 cttcggcaat gcaaagacat tcggcatggg cggcaaatac ggcgcggtaa gtttcctgca
    13501 aatcctgtcc ccggagctgt cggacgatat gctggccgac ttcctcaata cggaaagcgg
    13561 tgttttagtc aatctccatg tgcaggccat tgaacagacc aaggcgatca agaccatcaa
    13621 gcgcaaaatt accgaccttg acgctatgaa gattgcggag cagaaaaagg ccgtccgcag
    13681 cggttacgat atggacattc ttcccagcga ccttgccacc tacggcgagg acgccaagaa
    13741 gctgctgaca aagttgcaga cccgcaatga acggctgttc cagttgacgt tccttgtcct
    13801 caacgtggcc gacaccaagc aaaagctgaa taacgacgtg ttccaagccg caggcgtggc
    13861 ccaaaagcat aactgtcccc ttgtccggct ggactaccag caggaacagg gccttgcaag
    13921 cagcctgccg ctgggcgtca atcagatcaa gatacagcgc agccttacga cttccagcgt
    13981 ggccgtgttc gtcccctttg tgacgcagga gctttttcag ggcggcgcgg ccatgtatta
    14041 cggcatcaac gccaagtccc gcaacatgat tatgctggac aggaagcagg cccgttgccc
    14101 caatgccttg aagctcggca cccccggcag cggcaaatcc atgagctgta aatccgagat
    14161 cgtcagtgtg ttcttaacga cgcctgacga tatttttatt tcagacccgg aggccgaata
    14221 ttaccccctt gtcaaacggc tccacggaca ggtaatccgg ctgtcgccta ccagcaagga
    14281 ctttgtgaac ccgctggaca tcaacctgaa ttattcggag gacgataacc cgctggccct
    14341 gaaatccgac tttgtgctgt cgttctgtga gcttgtcatg ggcggcaaga acgggctgga
    14401 agccattgaa aagaccgtga tagaccgggc tgtgcgcgtg atctaccggc cctatctggc
    14461 agacccccgc ccggagaata tgccgatcct gtccgacctc cacaaagcgt tgttagatca
    14521 gcatgtcccg gaggctgacc gggtggcgca ggcccttgac ctgtatgtgt ccggctccct
    14581 gaacgtattt aaccaccgta ccaacgtgga catcggcaac cggcttgtgt cctttgacat
    14641 taaggagctg ggcaagcagc tcaaaaagct ggggatgctc attgtccaag accagatatg
    14701 ggggcgcgtc acggccaacc gaagccaagg aaaggccaca tggtattttg ccgacgagtt
    14761 ccatttgctt ttgaaagagg aacagacggc ggcctattct gcggagattt ggaagcggtt
    14821 taggaaatgg ggcggcattc caacaggtgc cacccagaac gtcaaagacc tgctttcctc
    14881 cccggaaatt gaaaatatcc ttgaaaacag cgacttcatt acgctgctca atcaggcgtc
    14941 cggggataga aaaatccttg cggaacgctt gaatttgtcc accgaacagc agaagtacat
    15001 cgacaattcc gaacccggcg agggcctttt gatctttgaa aacgtggtgc tgccctttac
    15061 caaccccatc ccgcacaaca cccagcttta caagattatg acgacccgct taaacgaggt
    15121 ggcgggagta tgacccggcg acgccacaaa ggaggtgaac caccatgaaa gagctgaaag
    15181 ccaaggcgaa agtgacgcag gccatgaccc gcgacgggct gacgctggaa aatcaggcca
    15241 ccggcgaggt ggagaacatt tccagccggg aggccgaaca ggatttgtcc gcgccggggc
    15301 cgggcggcac cgccgataag ctgctggaac gggccgacgc catccatgac ggccataagg
    15361 ccaagaaagc cgcgaagcag gccggggctg tggccgccga gggggacgct gccctgcacc
    15421 ggccatccgc ccggctccaa ctgtcggagg aagaacgggc cgacccggat ttacagccct
    15481 atatccgaaa atcggacaaa cgggccgata agctggataa ggcccgcgcc gccatcccca
    15541 agaagcgcgt cccggtcaag gaaaccgtct ttgacgaggc aagcggcaaa gcaaagaccg
    15601 tcctgcactt tgacaaacag gacaaggggc ctccctccct gaaacccaat ccggcaggcc
    15661 gtcctgtttc cgaggttttt ctattcggcc acgggaaaat ccatgaagtc gagcatgaaa
    15721 acgtgggtgt ggagggcggc cataaggggg aggaacttgc cgagcggcag gccggaaaag
    15781 ccatccgcag caccatccgg caccacaggc tgaaacccta ctgggacgcg gaaaaggccg
    15841 agcgcaggct attgtctgcc aacgcggagt atttctacca gaaatctttg cgggataacc
    15901 cggagatcgc acaggcggcc agcaacccca tttcccggtt ctggcagaaa cagcagatca
    15961 agcgccagta cgccaaggcc gcccggacag cgggccagac cgcaggcgcg gcggcacagg
    16021 gcgcggccaa gacagccggg gcctccacga aagcggccaa ggccgcagcc aaagaaagtg
    16081 aacgggcggc ctcctttgtg gcccgccact ggaaaggggc gctggccgtc cttgtgttgg
    16141 ggcttttgat gctgcttatc atcggcggct tacagtcctg caccgccatg ttcggcagcg
    16201 ccggaagcgg catcacggcg tcctcttact tttccgagga tagcgatatg ttggccgccg
    16261 aggacgccta cgcggagctg gaagccgagc tgcaagacta tctggacaac tacgaggcca
    16321 cccatgacta tgacgaatac cattttgacc ttgacgagat cagccatgac ccctatgtgc
    16381 tgatttccat tctgtcggcg ctccatgacg ggatatttac catcggggag gtacagggtg
    16441 atttgtccat gctctttgac aagcaatata tcctgacaga aaacgtcgtg gtagaaaccc
    16501 gttaccgcac cgaaaccgat acatggacgg acgcggaggg caacacccat acagacacct
    16561 accaagtgcc gtatgactat tacatctgct acgtcacgct ggaaaacttc gacctgtccc
    16621 atgtgccagt ctatatcatg gacgaggaaa cgctaagttt ctattccctg tatatgtcca
    16681 cgctaggaaa ccggcctgat ctgttttccg gcagccagta ccccaacgcc tccaccctga
    16741 cgccgccgac ctactacgac gtacccccgg aggctttgga ggatgccacc tttgccgcca
    16801 tgctcaaaga ggcggaaaaa tatatcggtt atccctatgt gtggggcggc agctctccca
    16861 gcaccagctt tgattgcagc ggcttcattt cgtgggtaat caatcatagt ggatggaacg
    16921 tgggacggct gggggcgcag ggcctatata acatctgcac ccctgtttcc agttcgcagg
    16981 ccaagcccgg cgaccttgta tttttcaagg gaacctacga cacccccggc gtgtcccacg
    17041 tcggcctgta tgtgggcaac aatgtgatgc tgcattgcgg gaacccgatt tcctacacga
    17101 accttacttc aaactactgg caatcccatt tttacagtta cgggcgttta ccttaacgcc
    17161 agaaaggagc tttcaattat ggcaaccaaa cttgaccgca tcgaaaagga catccagaaa
    17221 accaagtcta agatcgcgga gtttcagaaa cagcttcggg agctggaaac gcagaaaacc
    17281 gaacaggaga atttgcagat catccagctt gtgcgcggca tgaacatgaa gccggaggaa
    17341 tttgccgcgt tcttacgcag cggcgcaatg caggccgccc ctgccgcaac cccttaccat
    17401 gaacaggagg acaatgccca tgaagaataa gaccagacgg atttcccgta ttctccccgc
    17461 acttctggcc gtgatgctct gcgtcacggc ctttcccgtt accgcccttg caggcggcaa
    17521 tgacccggca ccggccccgc tgccggaggc tacaaccgaa cccgctacgg gcggtttgga
    17581 gcctgaaaca gacgaaaccg gcaccgccac ggacgggaca gaaaccgagg ccgaaaaaga
    17641 taccggccct ctgaccggca aggacatcac cgacctgttt tccgcgctgt tcggttccaa
    17701 ggtgagcatt gccgccacgg acgacggcat ccagatcacc accggcaccg acaaaaaaga
    17761 acctgaacag accggcaccg tcaccaccaa cggcggcagg ctgaatgtcc gcaccggcgc
    17821 ggggcttgac aagaccgcgt tcacccagct tcccaatggg acgacggttg aggtagtcgg
    17881 cacagacggc gactggatta aaattctctt gccggagcgc atcggctatg tccattccga
    17941 ctatatgacc gtcagcgaaa aggaagtagc ggctaccggc gagggcggtt tttccctctc
    18001 cattgacccg gaggaaattg cttctctgct ggaactgttc aatggcggcg gggccagcgc
    18061 cgccctgtcc ccggacggga acctgtcact gattgacgac atcggcagcg ccgcgaaaaa
    18121 tggcaagcag tttatcaccg tggaaaccaa aaacggcaat gtgttttatc tgattattga
    18181 ccgggacgac gagggaaaag aaactgtcca tttcttaaat caggtggatg aagccgatct
    18241 gctgacgctg atggaggacg gggaaaccgt cacccccgct gctgtctgct cctgtaccac
    18301 gaaatgcaag gccggggctg tgaacacgaa ttgcccggta tgtaaaacca acctgaccga
    18361 gtgcagcggc ccggagccgc aggaacccca gccggaggaa cccgaagccc cggaggaaga
    18421 acccaagggc ggcgcgggcg gcctgatcgt gttcctgctg gtggcgctgg cgggcggcgg
    18481 tgcggccctt tactatttca agttcaaaaa gcccaaggct gaaaccaccg gcagcgacga
    18541 tttgggcgag tatgattttg gggaggacga ggatttggag gacgaggaac ccgaaaccga
    18601 gtttgacccg gaagctgatt ttgtgagtga acaggaggac gaggaatgac atataaactt
    18661 gtaatcgcgg aaaaacccag cgtggcccgc agcattgcgg gcgtgatcgg ggccacggag
    18721 aaacacgacg gctatttgga ggggaacggc tatctggtga gctggtgcat cggacatctg
    18781 gtgtcctttg ccgacgccgg acgctatgac gagcgtttca aaaaatggcg ctatgaggat
    18841 ttgcccattc tcccggaaac atggcagtac atcgtccccg acgacaagaa acagcagttt
    18901 gacgttctct gttcgctgat gcagcgcccc gacgtgaccg gccttgtgtg cgccaccgac
    18961 gccgggcgcg agggggaatt gattttccgt ttcgtctacc agatggcggg atgccaaaag
    19021 ccctttgagc gcctttggat ttcctctatg gaggacagtg ctattaagga cggctttgcc
    19081 cacctgaaac cgggcggcga ctatgatccg ctgtatcaat cggccctgtg ccgggccaag
    19141 gccgactggc tgatcggcat taacgccacc cggctttttt ccgtcctgta ccacaagaac
    19201 ctgactgtgg gccgcgtcca gacgccgacc ttaaaaatgc tggtggacag ggacgccaag
    19261 attaccgatt tcaagaaaga gaaatatcac atcgtccata tcacggcggg cggcgcggac
    19321 gcggtaagca gccgcttttc cgacgctgcc gaggcaaaca ccgtcaaggc ggcttgtgca
    19381 aaagcgcagg ccgtttgcgt ttccgtgacg cgggagaaaa agacggaaca gccgcccaag
    19441 ctctatgacc ttaccacctt gcagcgggag gctaaccggc ttttcggcta taccgccaag
    19501 cagacccttg actatgccca ggcactgtat ctaaagaagc tgctgaccta tccgagaacg
    19561 gatagtcaat atcttaccga cgatatgctg cccgccgtgg aaagccttgt gtccggcctg
    19621 tgggagctgg tgccgtttgc caagggcctg aacctttccc cgcagtttga ccgcatcctg
    19681 aacagcaaaa aggtgtccga ccaccacgcc attatcccca ccgctgaatt tgtgaagcag
    19741 ggctttggca ctctggccga gagtgaaaaa aagctgctgt ccctgatctg ctgcaagctc
    19801 ttatgtgccg tggccgggcc gcatatctat gaggccgtca ccgccacatt tacctgtgcc
    19861 gggcaggagt ttaccgccaa gggcaagacc gtcctttccc ccggctggaa agagattgaa
    19921 cgccggttcc gttcttcctt aaagaccgac gccgacgagg acgccgaggc ggtgcgggag
    19981 ctgccggagc tttgcgaggg ccagaccttt gcagacgtgg ccgcctctgt caccgagcat
    20041 ttcaccacgc cgcccaagcc ctacaccgaa gatacgcttt tgtccgctat ggaacgggcc
    20101 ggggccgagg atatgccgga ggatgccgag cgcaagggat taggcacacc ggccacccgc
    20161 gccgccattc tggaaaagct ggtgcagatg ggctttgtcc agagaaaagg caagcagctc
    20221 attcccacca aggacggcat caatctggct gtggtgctgc cggaggcgct tacttctccc
    20281 cagcttactg ccgaatggga aaaccggctg accgagatcg ccaaggggca ggccgatcct
    20341 gacgaattta tggacggcat cgagacgcag gcgcgggagc tggtgcagac ctattcctgt
    20401 atcagtgagg aaaagcagaa attgtttcag gccgagcgtg ttcctatcgg gacgtgtccc
    20461 cgctgtggtg aggctgtcta tgagggcaag aaaaactact actgcggaaa ccgggcctgc
    20521 cagttcgtca tgtggaagaa tgaccgattt tttgaggaac gcaagaaagc gttcaccccg
    20581 aagatcgccg ctgcgctgct caaaaccggc aaagtcaagg taaagggcct ctattccgta
    20641 aagaccggca aaacctacga cgggacggtg cttttggctg ataccggggg caagtacgtc
    20701 aactaccgca ttgaacacag gagctaaagg acagtaacac agcaagcata gggagtgtgg
    20761 ctacactccc ggtaaaatcc cccggccgcg tccctgccgg gccggggatt tttgcttgtt
    20821 ggggaagtcc ccaaccccct attttttgaa acagaacaga gaaaggagtg accgcgtgaa
    20881 cgaagtattt tctatccaag tccataaccg gcagtttttg gaacagggca aacaaggcgt
    20941 ctggctggat ttgcccacca caaccgaaac gctgcaagcc gccctgcggg atgttggcat
    21001 tactgccgac aatccgcagg atttttttat caacgggtat tcctgcccgg aggacagaca
    21061 ccttgccatt ccctatgaca tggtgctggc cgccgacgtg gacgagctga atttccttgc
    21121 cgcccggctg ggccagcttg acgccgccga gatcgcagag ctgaacgccg ccctgcaaaa
    21181 tccccgtggc ggctttgaga atatcggcca gatcatcgac tacaccgaaa atgtggatta
    21241 ctatgtgcat cttcccgacg ttagggggga tggacagttg ggcgactact acctgaaccg
    21301 ttccggcatg gtggatatgc cggaggaatg gaaagccggg attgatacgg cgcagtttgg
    21361 acgccacatt gcccagcagg aacaaggcgt ttttacccag tacggctatc tggtgagaag
    21421 cggcgacgaa tggcagcgcg tccatgaggg ccagccggtg ccggaggaat accgggtgat
    21481 gcactacccc cagccggaag ctgaccggga ggaaccgcag gcccagcagc cggaggcatc
    21541 cgaaagcccc gacaaagcgc cgcccccggt tatcccgatc atgctggacg gcatgaacag
    21601 caaagaacgc atgaaagtga ttactgaccg tctggaaacc ggcgtacagg agctttttga
    21661 aagtagccgc tatatggctt acttatccgc tatgtccaag atgcacgatt acagtctgaa
    21721 caatacgctg ctgatcgcca tgcagggcgg ttcgattgtg gcgggctata acaggtggca
    21781 ggatgaattt gaccgccatg tgaagcgggg cgagaaaggc attaagatac tggccccggc
    21841 cccgttcaag gttaagcagc agatggagaa aatcgacccg gccacccagc agcccgtgat
    21901 cggcgcggac ggcaagcccg tgaccgaaga acaggaagtg acggttccgg cctttaaggt
    21961 ggtgacagtc tttgacatca gccagaccga gggcaaggat tttcccatgc cctatatcag
    22021 tgaacttacc ggcgacgtgc agcagtatca ggatttcttt gccgcgctgg aacggacatc
    22081 accctatccc ataagcattg agccgatttc agacggggcc aagggccgct gcttctacgc
    22141 aaaacagcgc attgccatca acgagggcat gagcgaattg cagaccctaa agaccgccat
    22201 ccatgagatt gcccatgcca agctccacgc cattgaccgg gaggcccccg ccgaggaaca
    22261 ggcggcccgg ccagaccgca gcacccgcga ggtacaggcc gaaagcgtgg cctataccgt
    22321 ctgccagtat tacgggctgg acacgtcgga ctattctttt ggctatgtgg ccgattggag
    22381 cagcggcagg gagttatccg agctgaaagc ctcccttgaa accatccggg ccacggccag
    22441 cgaattgata accgagatcg acggccattt tgcccagctt cagcaggagc gcgaggcccg
    22501 gcaggaacag gaggccgtca cggttgacgt taccccggag gcagcggccc cggcgcaggc
    22561 cgaggaagaa caggccccgg cccttgatcc ggcagccgag ccgcttgtga ctgtgctatg
    22621 gagcgaaagc ccccagctaa aggacggcca gcaaatgccg ttgcatgagg ccgacgcgct
    22681 gtttgcccgt ctggatgcag catatccggc agaggcaggc tatgacaaaa ccgcgttccg
    22741 cattgactgt atctttcagg gcgagccgga cagctacgag gggcggcagg attttggaga
    22801 tagggacggt tccctgatcg aacatatcca gagctaccac caattttacg cgcaggacga
    22861 aaactggaaa aagcatgtaa tcgctacaac cggcatagag ggctgggagc aggacaaggc
    22921 ccatcgggat atgctgttag gcgagtttgt cccctatatg aagctgcatt gcagccttgc
    22981 ccaaatggaa cagacggcgc agggccgctt acagtccggg gaaaccctga cgccggagga
    23041 aacggcctat ttgggggcgc ttctctctta tgtgcaggag tgccgcccca tgctcaatca
    23101 gggccagtat cagttgccgg agccgccgaa actggctgac ttcgaccaga gcttgcagga
    23161 ctacaaagca caggtacagg ccgagatcgc gcaggaggcc gccgcagccg gtatgaccgt
    23221 ggaggaatac gcggcggctg gctatgaagc accccaacag gacacctttt ccatctacca
    23281 gcttcgaggc ggggaggaaa cccgcgacta tcggtttgag ccatacgagc gtttacaggc
    23341 cgcagggctg gccgtggaca gtgccaacta tgaccttgtt tatacggcac cgctggataa
    23401 aagcacgtct ttggaggata tttaccgcac attcaacatc gaccacccgg cagattttag
    23461 gggacacagc ctttccgttt ccgacgtggt ggtgctgcac cagaatggac aggacaccgc
    23521 ccactatgta gacagcttcg gctatatgga agtgccggag tttttgcggg aaaatcccct
    23581 gcgtaccgcc gaactgtcta cggagcagaa cgaaaacatg attgacggca ttatcaacaa
    23641 tacgccgccc gaaccgcagc cggaggaacc cgaacagcta tcttttgagg atgtggcagt
    23701 tgcttccggc cagacacagg aggaaccccc ggaggctgcg cccccgcaaa cggctgtccg
    23761 ctattatccc atcaatgagg gagccgcccg ccgcgccaag gaagccatga gcttttctga
    23821 ctaccggccc ggcagcgcca cggcggaata ccggcactat gtcgatcagg ccgtggaaat
    23881 tgcggagcgc cagaaaaagc gtgtggagcc agagtatcac gaaaagatag acagcctcct
    23941 tgacacctac gcccgcaagc tggccgagaa catgaataag ggctatgaga ttaccgcccg
    24001 cgtcccctct gtcctgattg ccgggccgtc caatttcccg gtacgggcga aagaaaagca
    24061 gaacgccgcc agtgaccgca acatggagga atttcagtac attcagggct tgcttgacaa
    24121 aatccgcagc accggcatgg gtggtatcag cgccgacgat ccgcaggccg tttccaagct
    24181 ggaaaagaaa ctggaaaagc tggaagcgtc gcaggagttg atgaaggccg tcaatgccta
    24241 ctaccgcaag catgggacgc tggacggatg cccccatctg accgaaagag gcattgaaaa
    24301 cctgaaagcg gatatggcgt cgggctggca ctatgagaaa aaaccgttcc agtcctggca
    24361 gctctccaac aataacgccg agatacgccg gttaaagggc cgcattgagg aattgacccg
    24421 ccaaaaggag gcggcctatg tgggatggga gtttgacggc ggcactgtgg agatcaaccg
    24481 ggtggccaac cgcctgcaaa tcttctttga ggggaagccg gatgctgctg tacgcgacga
    24541 gctgaaaagc aacggtttcc gctggtcgcc caaggccggg gcatggcagc ggcagttaaa
    24601 cgacaccaca atccgcgtcg ctgaccgtat caagtgcatc cagccccttt ccggcgaaaa
    24661 gcccagcgcc ttacagcttg cggcccgcag ggagaaagaa aagccctcta tccgggcgca
    24721 gctcaacgcg gccaagaccg aacaggccaa gcagccaccg gcgcgggaaa aatcaaaggg
    24781 attggaggta gagtaaatgc agaagtttga aaatgtggac gttctggccg cgctggaaca
    24841 gatcatgcgg cagaacaccg cgttttatca aagtgacttt ggcattgaca aagacattat
    24901 caggcgggat gccgccagcg atcaggccgc agacaagacc ctgctctgga tgtcccggcc
    24961 ctccgggacg tactgtttcc gggaacgtga tgttttcctg aaagacaccc gacagtacaa
    25021 cacatggaaa ttttacgggg agcagacccg tgacaagata ctggcctacg cggtggagct
    25081 gaccggcaca cagggcggca ctatccgggg caatctctat gagctggact atccgcagca
    25141 tttccggcac gtcattgaaa ccgcccagcc ggtgagtgtg aaccggctct tttatgagca
    25201 tggcacccgt gacatcccgg agagccagcg ttttgacgga agccctgacc gggtattggg
    25261 aaactttttg tactatgagg cccagcccca tgacccggct gtgctgcagg aggcgctacg
    25321 gacggagcag cacagccggg ggcgggccgt ccccggcgat ttcaaagtcc acgtcaccgc
    25381 tttgcatgac agccggattg aaaccgaggc ccggcgtatt gtggacagcc tgaaagatct
    25441 gtccgcgcct aacagcccca ataaaacgca tttcatggtg gagatctccc cgcactttgc
    25501 ggcgattgct tccaccaagg ataatgaccg gctctttgcc atgctgccct ataaatccct
    25561 gtgttttacc gggatgaaag gcaggcatgg cgtgtatgcc gtgattaacc gggacgagcg
    25621 ccgggatgtg agtattcgca ggccccggcc ctctatccgc aagcagctat ccgacagcaa
    25681 acaggccgcc agcccaaaaa aggcggcggc cagaaccaaa aagaatgaat tggaggtata
    25741 aggaatgatg cactttaccg tggaggaaga aaacctgatc tgtatgtacc acaatgcaga
    25801 ccggcgcaga acgataaaca ggctcaccgc cgtcatgccg gttatggatg gggatatgcg
    25861 ggcgcaggcc cagcagaccc tttccaagct ggaacgcatg acggatgccg actatgacgg
    25921 ccagaagttc cacttcacag acgaataacg aacagcagac gccgggcgcg gcggccatgt
    25981 ggtcgcttgc gtccggcgtc ttttcttttt gcctattcct acgcatttag aacccggtat
    26041 tgtcggggat aggatgaagt attcgcttgc ccctgcaaac atgcgggaga agccttgcag
    26101 cgggcggttt tcaatccctt aaatgcgtag gttgcagcta taattagacc cctgaaacag
    26161 atatagccgc gcagcgggtg tatctgtttc aggggtgtgc aggtgatagc cgcaaagcgg
    26221 cgtaagaggg tgcagccctc ttgccgtcta tctctgttcc agcgggaagc tcggcaatgc
    26281 ttccagcagt ttcagggaaa tgcgctgccg tatatcttcg tcaatgactt tcttcttttt
    26341 gccgtttgcc tgtctgatct ccaccgtgga aagctcgttg atgtagtcag cataatgggc
    26401 caacactttt tccgtggccc acctctcacc ggcaacggcg gctttgatcg tttcatagtc
    26461 cagcagcttt tcttgtccgt ccatcctgat acccccgtca gactgatttt ttctatgata
    26521 ccatattcgg aagcatggaa ccagagaaaa cggcgctttt ctgctggtaa attctacgca
    26581 tttagaacag cacttcggcg gggataggat aaaacaccca cataccccgc tgaccatgcc
    26641 ggaaaagcct tgaaatataa gggcttttac agctctaagt gcgtaggttt ccagtatctt
    26701 ttccgctggc gctcggcctc tttgcggcgg cgcatccgtt tggagcaggc cgcgcagtat
    26761 ttggcccggt tagacttcgg cgtgaacgtg gccccgcact cacagcagcg tttcacttcg
    26821 tcccggtctt tggtgatctc ggcatacagg gccgcatcca gcgggaggac agcggcctta
    26881 aaccagcggc acagaagcga ataggaaatg ctctgtacgc aaatacattc ttcgccgtcg
    26941 tccagcaaca ggcagttgcc gtcacaatag ttgcagcact catgcgtcag cttccgggcc
    27001 ttgcgatatt gagggtaatt cattcgtgga atactcatag ctcttgcgtc cattcctttc
    27061 ccggcgtcat acgcaaaatg ccgtccacat tctgcttgat gatgccgtat tcctgcatct
    27121 gctttttcgc ttcgccgtac ttctcataca gccgttcctt ttcctcggcc aatcgcttgt
    27181 actcggcttt cagggcgtac agatcgggga gctttttcac accctgtccc tttagggcct
    27241 ttgccgcagc ttcatacaaa atcaacgtgc tttcatgctc acgccggaat tttgccttat
    27301 ccgctgcatt gcggtattcc atagccacgg gccagagctg cttgtaggta gaaatattct
    27361 ttatcagcac cgccatatcc gcaagccggt gttccatagc tttgagggta gcggcggcct
    27421 cgtcgctggc ggcggtgatc tcggccactt tgctttccaa atccgggtag gtgtctatcc
    27481 catgttcagt caggaaattc aaagtctttg cggcctgctt caaattgtgg agcttcgccc
    27541 acctctcata gcctgccgac tgctgggcct taatgttgtt ttccagatca atccgcaaag
    27601 aaatcccctt gcgctctttg ggcgctctgg tgataacgcg gcctttgata cgctctttga
    27661 tggcttcctc ggtgtaggct tcccccaatg tcttgcagcg ggtaaagcgt tcctgtccgg
    27721 gagcgcggaa agaaataaac tttcccggct tgatctcata gccctgcgcc tgcatgagcc
    27781 gcaggaaatc atcaaaatct tttgcctgcg ggatagcggc gtcaatggct gctttgagct
    27841 tcgctttcca gctcgtccct ttccgctggg cgtcccactc ggcatagctc ttgcccttac
    27901 tctggccggg cttcaccacg gacaatccgt tttccttgca cagcctgtcg ctggtgcgcc
    27961 ggatataggc atagctctgt ttgttggaaa tgtacttgcg ctgatttacc atatccacgg
    28021 cacagaaaat aatgtgattg tggacgtggc ccttatcaat atgggtagtc agcacatagg
    28081 gatatttccc ttgcagcact tcctcggcaa gctgcttccc gatctcatgg gcctgttccg
    28141 gcgtggcttc gccgggggca aaggattgta tcaggtggtg gcccagatta ttgccctttt
    28201 ggtatgcctg cgccagcagc atttcaaatt caatgtccgc tgtttcataa gagcaggcaa
    28261 aggaggccac cagcagcttg ccgtcagttt tgtccgggtt ctcgatgtag tcaagggctt
    28321 ttttcagcgt tgacttgata ggatgtatct ttgtaactgc catatctgcg ccagcgcccc
    28381 ctttatatcc tcgatgtcct gtgcgtacac cggccccata ccgttcagcc tctttgcaat
    28441 ctgattgacg ttgacgccga tcttctgtat ctcggcggtc tgcgcccgga tgtcgctggt
    28501 gtccatgttg acgatatagc cgtcaatgag catcttccgg gcgtaggctg aaaagttctt
    28561 cgtatgcagc agcgccattt tctgttcaat catagcccgt tcctccggcg tcacagggac
    28621 gcgcagcacg atttttcgct ttctgtttgc catgcttcac cgtcctttcc attcaggggt
    28681 ttggggattt ccccaacaag caaaacggac gcggcccggc agggacgggg caggccgttt
    28741 ttccgggagt ggctacactc cctatgcttg ctacgttact gtttctccac taacaagacg
    28801 tttccaaagc cccaaactac ccgcaaaata gccctctgga aagaaaaaag tgcaaaaaaa
    28861 tagaagctac gctgtgcagc tcccgctaaa agtaaaacag aggatgccag ttacacatcc
    28921 tccgtttcat tcttcttcgg cttttcaatt agtatggcgc tgtcaatcgc gccctcgatg
    28981 atcggcaggt attcttcggg gcagagtttc agcttgtggc tgaccctttg ccgcagttcg
    29041 ctttcctccc gcatgatctc cgggttaaag tagcgttcca caggaagccc gcaggcttta
    29101 ataagctgta tgacaaccgg cagactggga atggccccgt cgttttcgat gttggcaaga
    29161 tagcgccagt caatatttat tttctctgcc attgctttgc gcgaaacgcg ctttgcttgc
    29221 cgggccgctt tcacatccgc gccgaaagtt tcaaagccgg gacaatcttc aatattcgcc
    29281 atatcgcacc tcccacttac attgtatatt tcatactctg cccgtggaat gttggtatat
    29341 gcacctttct taaagtttat atttcacatt ttgtttcttt tattcttcga gcaaaagaat
    29401 tataatgtac cctgtggagg tgtactatgg actacatgac gttgaaagag gcaggcgaga
    29461 aatggggtgt gcccccacga tggataaact actattgtgc tgcgggacgc atccccggcg
    29521 ctgtaaagat ggcgacgatc tggctcattc ccaaaactgc ggaaaagccg attgacaggc
    29581 gtacaaagaa aggaaaggcg gttactgaat agtataatga aaccggcgca ttttggatat
    29641 ggttttgaag agagagaaag catggtattt tgtcacggaa aaactataag atgatagatg
    29701 gcaggctgct ccagaccgat aaaaagtatt ctcatctgaa acagaaagaa aagatttaca
    29761 catggatgta tgaagaagcg aaactttatc atgacagaaa tggaaaatgc ccgaaacaaa
    29821 gacgaggaag ctgctgctgt atcagccgta tatggtagaa ttgaaaaggc cggcatctgg
    29881 attctttatg gtgaagtctc cagacattgg ataactgctg gtctaatgcg cctttgagta
    29941 tggggtgatt gattttaata ccggggaggt acggcttttt gaggaaaata ttctgggatt
    30001 tacatttggc gattattaca tttcctgtaa ctgcaaggac acggatgatg aagatatgag
    30061 gaatatagac ttgctgattt tctactgtct tgaacggtat taggactgaa tctgctgcgc
    30121 ggagtgcggc aagagcgacc ggagggagta cacacaggcc ctgcctgtgt gaaaagccat
    30181 accgcaaggt gcattcgcaa aaaagaggcc gcgcattttg ctgaggcttg gggtgtcccc
    30241 aacaagcaaa acgagaacgc cgcaggcggg caagtggtgg atcagaatat ccgtaaagga
    30301 tattctgatc cattgcttgc cagtattatc gggatgtttt gacaaccgct gtaatcgaaa
    30361 catcccaaac attaagaagg tgttaattat tattatcttg atttaattcg tttaaaccaa
    30421 taatatagat acatacacca tgaaaagaag cgagtatccc aatgacggat gattaaaatc
    30481 cgaaaagaaa tcgctttgaa actacaggga aactacagac tgttatgtta tcttcttaaa
    30541 atggagggat ttttatgtcg atacgaattc tacttgtcga ggatgatgat catatctgta
    30601 atacagtaag ggcgtttctg gctgaggcag gatatcaggt ggatgcctgc acagatggaa
    30661 atgaggcata caccaagttt tacgaaaaca cttatcaact ggttattctt gatattatgc
    30721 tgcccggtat gaacgggcat gaacttttgc gtgaatttcg tgcgaaaaat gatactccca
    30781 ttctgatgat gacagccctg tcggatgacg aaaaccaaat ccgggcgttt gatgcagagg
    30841 cagacgacta tgtaacaaag ccattcaaga tgcagatttt actaaagcgg gtggaagccc
    30901 tgctgcggcg cagcggtgcg ctggcaaagg aaatccgtgt cggcaggctg acacttctgc
    30961 cggaggattt cacggtactt tgtgacggta cagagctgcc cctgacacga aaagaatttg
    31021 aaatcctttt gctgctggtg cagaacaaag gcagaacctt aacgcatgaa atcattttgt
    31081 cacgtatatg gggatatgac tttgagggtg atggcagcac agtccacact catatcaaaa
    31141 atttgcgggc caagctgccg gaaaatatca tcaaaaccat tcgcggtgta ggataccgat
    31201 tggaggaatc ataataatgg aaagaaaagg gattttcatt aaggtttttt cctatacgat
    31261 cattgtcctg ctactggttg tcggtgtaac agcaacactg tttgcacagc aatttgtgtc
    31321 ttatttcaga gtgatggaat tacagcaaac agtaaaatcc tatcagccat tggtggaact
    31381 gattcagaat agcgataggc ttgatattca agaggtggca gggctgtttc actacaataa
    31441 ccaatccttt gagttttata ttgaagataa agagggaagc gtactctatg ccacaccaaa
    31501 tgccaataca tcaaatagtt ttagacccga ctttctttat gtggtacata gagatgataa
    31561 tatttcgatt gttgctcaaa acaaggcagg tgtgggattg ctttatcaag ggctgacaat
    31621 tcggggaatt gttatgattg cgataatggt tgtattcagc cttttatgcg cgtatatctt
    31681 tgcgcggcaa atgacaacgc cgatcaaagc cttagcggac agtgcgaata aaatggcaaa
    31741 cctgaaagat gtaccgccgc cgctggagcg aaaggatgag cttggcgcac tggctcatga
    31801 tatgcattcc atgtatgtca ggctgaaaga aaccatcgca aggctggagg atgaaatcgc
    31861 aagggaacat gagttggagg aaacacagcg atatttcttt gcggcagctt ctcatgagct
    31921 aaaaacgccc atcgcggcta caagcgttct gttggaggga atgcttggaa atatcggtga
    31981 ctacaaagat cattctaagt atctgcgcga atgcatcaaa atgatggata ggcagggcaa
    32041 aatcatttcc gaaatactgg agcttgtcag cctgaatgat gggagaatcg tacccatagc
    32101 tgaaccgttg gacatagggc gcacggttgc cgagttgctg cccgattttc aaaccttggc
    32161 agaggcaaac aaccagcggt tcgtcacaga tattccagcc gggcaaattg tcctgtccga
    32221 tccgaggctg ctccaaaagg cactatccaa tgtcatattg aatgcagttc agaacacgcc
    32281 gcagggaggc gaggtacgga tatggagtga gcctggtgct gaaaaatgcc gcctttttgt
    32341 tttgaacatg ggcgttcaca ttgatgatac tgcgcttcca aggctgttca ccccattcta
    32401 tcgcattgat caggcgcgaa gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt
    32461 acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct cggatggcgt
    32521 tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa cttaaatgat
    32581 tttgaccgac aggtataacc ctgccggtct ttttttgctg ctacaggaaa actacagact
    32641 ggctacaggg aaagtacaga tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt
    32701 agttttattg caaaggaggc attcgatcaa atggaaaaaa gcaactatca ttccaatgcg
    32761 gatcatcaca aacggcatat gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc
    32821 attatttcgt tcacagtctg cacgctgttt ttgaggtgga gactggcttc cgtattggag
    32881 gcaacacaga taccgcccat ccctgcaact catacaggca gcagcactga cgtagtggag
    32941 aatttggagg aaaacgctct tgccaccgcc aaagaacagg gagatgaaca ggaatggagc
    33001 ctgattttag tgaacaggca gaaccccatc cccgcacagt acgatgtgga gcttgagcaa
    33061 ctatcaaatg gtgagcggat agatattcgg atttctccct atcttcaaga tttgtttgat
    33121 gccgcaagaa ctgatggagt ttacccgatt gtcgcatccg gataccgaac aacagaaaaa
    33181 cagcaagaaa ttatggatga aaaaattgcc gaatataagg cgaaaggcta cacctctgca
    33241 caggctaaag cggaagcaga aacttgggtg gccgtgccgg gaacgagcga gcatcagctt
    33301 ggtcttgctg tggatatcaa tgcggacgga attcattcaa caggcaacga ggtttataga
    33361 tggctggatg aaaacagcta tcgttttggt tttattcgtc gctacccacc agacaagaca
    33421 gagataaccg gtgtgagcaa cgagccgtgg cattaccgat atgttggcat cgaagctgcc
    33481 acagagatgt acaaccaagg ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa
    33541 aaggatgtta tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa
    33601 tgagacgggc gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct
    33661 atgcgcagac gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat
    33721 tatacaacca caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa
    33781 agctggcagc aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct
    33841 ggtggctggt acgccatgcg gacaaagata ccccctataa agacggcctt acggtgacca
    33901 atggtaaact caccaccatg tcgggcggcg gtatgtgcca gatgagcaat ttactatttt
    33961 ggatgttcct gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt
    34021 ttccagagcc aaacagtgac gagattaaag gggtggatgc aaccattcca gagggctgga
    34081 ttgatttaaa agtgcgaaac gataccgact gcacctacca aatatgggtg accttagatg
    34141 atgagaaaat catcggtcag gtgtccgccg acaaagagcc ccaagcatta tacaaaatta
    34201 caaatggcag tatccagtat gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta
    34261 aacggatgca agttgcctta ggtaccgggg aaataataga ttgcaagctg ctttatacaa
    34321 acaaatgcaa aatctgttat cccctcccgg aaagtgtgga tattcaggag gagaaccaat
    34381 gagaaaaagt gtgggcatta ctgtttttgg atgcgaacag gatgaggcaa atgctttccg
    34441 cgctttatcg ccggattttc atattatccc tacgctgatt agcgatgcga tatcggcaga
    34501 caacgcaaaa ttggccgctg gcaatcaatg cgttagcgta ggccataagt ccgaggtttc
    34561 cgaggcgaca attcttgcgc tgagaaaggt cggggtaaaa tacatttcta cccgcagcat
    34621 cggctgcgat cacattgata cgactgccgc cgagagaatg ggaatctcgg ttggcacggt
    34681 tgcgtattcg ccggacagcg ttgcggatta tgctttgatg ctgatgctga tggccatacg
    34741 gggtgcaaaa cccaccatgc acgccgtggc gcaacaagat ttcagattgg atcgtatccg
    34801 ggggaaagaa ctgggggata tgactgtggg agttattgga accggccata tcgggcaagc
    34861 ggtcgtcaaa aggctgcggg gatttggatg ccatgtgctg gcctatgata acagccgaaa
    34921 aatggatgca gattatgtcc agcttgatga gcttctaaaa aacagcgata ttgttacgct
    34981 ccatgtgccg ctttgtgcgg atacccgcca tctgatcggt cagaagcaaa ttggagagat
    35041 gaagcaaggc gcatttttaa tcaacaccgc gcgcggagcg cttgtcgata ccggggcgct
    35101 ggtggaggcg ttggaaagcg gaaagctggg tggtgcggca ctggatgtgt tggagggcga
    35161 ggatcagttt gtttataccg attgctcgca gaaagtgctt gaccatccct ttttgtcgca
    35221 gcttttaagg atgccgaatg tgatcatcac accccatacg gcgtactaca ctgagcgtgt
    35281 gctgcaggat actacagaaa aaacaatcag gaattgtctc aattttgaaa ggagtttaca
    35341 gcatgaatag aataaaagtc gcaatcatct tcggcggttg ctcggaggaa catgatgtgt
    35401 cggtaaaatc cgcaatagaa attgctgcga acattgatac ggaaaaattc gatccgcact
    35461 acatcggaat tacaaaaaac ggtgtatgga agctatgcaa gaagccatgt acggaatggg
    35521 aagccgacag tctccccgcc atactctccc cggataggaa aacgcatggg ctgcttgtca
    35581 tgaaagaaag cgaatacgaa acacggcgta ttgatgtggc tttcccggtt ttgcatggca
    35641 aattcgggga ggatggtgcg atacaggggc tgtttgtatt gtctggtatc ccctatgtgg
    35701 gctgtgatat tcaaagctcc gcagcttgca tggacaaatc actggcctac attcttacaa
    35761 aaaatgcggg catcgccgtt cccgaatttc aaattattga taaaggtgac aagccggagg
    35821 cgggtgcgct tacctaccct gtctttgtga agccggcacg gtcaggttcg tcctttggcg
    35881 taaccaaagt aaacggtacg gaagaactta acgctgcgat agaagcggca ggacaatatg
    35941 atggaaaaat cttaattgag caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg
    36001 ggaacgagga tgatttgatt gtcggcgaag tggatcaaat ccggctgagc cacggtatct
    36061 tccgcatcca tcaggaaaac gagccggaaa aaggctcaga aaatgcgatg attacagttc
    36121 ccgcagacat tccggtcgag gaacgaaatc gggtgcagga aacggcaaag aaagtatatc
    36181 gggtgcttgg atgcagaggg cttgcccgtg ttgatctttt tttgcaggag gatggcggca
    36241 tcgttctaaa tgaggtcaat accatgcccg gttttacatc gtacagccgc tacccacgta
    36301 tggtggccgc cgcaggaatc acgcttcctg cactgattga cagcctgatt acattggcgt
    36361 taaagaggtg ataagtatgg aaaatggttt tttgttttta gatgaaatgt tgcatggcgt
    36421 ccgttgggat gccaagtacg ccacatggga taacttcaca ggaaaaccgg tggatgggta
    36481 tgaggtgaat cgcatcatcg gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat
    36541 ccatgcggct gcgcttggct atggcttgct tttatgggat ggatatcggc caagaactgc
    36601 ggtggactgc ttcctgcgtt gggcagcgca accggaggac aatctcacaa aagaaaaatt
    36661 ttaccccaat atagagcgag ccgagttgat tacaaagggt tatgtggcct cacaatccag
    36721 ccatagccgt ggaagcgcaa ttgatcttac gctctaccac ctggatacag gggaacttgt
    36781 ttcaatggga agtaacttcg attttatgga cgaacggtcg catcatacag caaaagggat
    36841 aggggatgca gaggcacaaa atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt
    36901 tcagtcttat cgctttgaat ggtggcacta taagttgatt gatgagccat accccgatac
    36961 ctattttaat tttgccgttt cataatgaaa gtattggttt tgtatcagat tttggatttg
    37021 ataaaatcta tgctggtcat ggacaatctt ttactggaaa gtggttaaag catcttttgt
    37081 aatacattgc aaattatagt attcaatctg cccagcggca gaaaagaaaa aaaccgctgt
    37141 tgggcagacc tttcccacgc ccattttgta ttttgctggc ggcggttttg agaaatcaag
    37201 gccgccgttc ttttctggca gcaaaaggtg tggcggccca taggaggatg ccgctatgct
    37261 gttttgctat tgccatgctc taatccactt ccaccactca aaaaagcctt gcccctccac
    37321 agggctattc cggttatgag tatgtactat accatgtaaa tcagcagcat agcaaagcat
    37381 tttctcgccc ggtaggtttg cgacctgccg ggcgattttt gtcgtctttt gcggcccggc
    37441 tgtccccaaa aaaatttttt gagaaatttt caaaaaggag gtatttagcg ggtagctggc
    37501 ggccttgtca aagtcttgtt agcggaaagg gagagaacca cctaagtccc ccacagaaag
    37561 ggggtgagaa gatggatgct atcccccgcg actatgaagc gcggtgcatg ttcgatgctt
    37621 tttgcaagac ggtactgcgt aacgaggcca aaagctactt ggctgaaatg aagcgccgcc
    37681 gtgaccgtga agtgtcacta agctctttgt cacaggcaga tttggacaag ctctgtacag
    37741 tagatcacta ccccagcgac agctttacgt tttcgtcgca tggctatgac ctacatatca
    37801 acaatgagct tgttgccgaa gcctttgccg cgctgcccag catggagcag agtattttaa
    37861 tcctgcactg tgttctggat atggcagatg gtgagatcgg cggccttgtg ggaatgtccc
    37921 ggagtgccgt tcagcggcac aggacaaata ccttatcgga gctgcggaaa cagctaaagg
    37981 cgttgatgcc gaagggaggt tagacgcgga tgaagaaccc gaactacaag ggcattgagc
    38041 ttttgcccct ttcggtgatc gaggccgccc gcgctgggga tgccggagcc gtagagcgcg
    38101 ttttgcagta ttacagcggt tacatgaaca agctgtgtac ccggacgctc tatgacgaaa
    38161 acggctatcc gcatgtgtgt ctggacgagg ccatgaagcg ccgtctggaa atcaagctca
    38221 tccacgctat cgtcacgaac tgacgaaccg gccccggcag catgaaacgg cttacccttt
    38281 ccccgtttcg gtgttttccc ggtggcctga tcgcaccttg ataaatatgc ccgcaagata
    38341 acggcggcgc atcatagggc atacggacac attctgtctg tgccgagccg gacggctggt
    38401 gcgccatgat acttttccct acgggaaaag cggagcgagg aacaccacgc cgcaatgtga
    38461 gcgtggcggc ttgcgggcga cgacgtacag gcaggactat gatactccct tatagccaca
    38521 gtccgagcgt taaaagcgtc gcaggcaatg ggtagggccg cgtgagaacc acgcgggggt
    38581 gaaagtcccg tggagctgtg ccagcagccg tccgtctatg cccctaaatc acacaaacct
    38641 ttgaatttgt gaaagggggc tgcattatga accatgatgt gcctatttgg gaaaaataca
    38701 cgctgacgat tgaggaagcg tctaaatatt tccgtatcgg agaaaacaaa ttgcgtaagc
    38761 tggccgagga aaatccggcc tctggctggg tcattatgaa tggcaaccgc atccagatca
    38821 aacgcaagca gtttgagaaa ataattgaca ctttggacac aatctaatag tgaaaaaggg
    38881 ccgcagatat gttataataa tttataagca tatatgcggc tctttccgtc atggaaagga
    38941 gcatgacgag atgtcagaaa aaagacgaga cagcaaaaat cgcgttttgc ggtctggaga
    39001 gagccagaga aaagacggac gatatgctta caaatatgtt gatacctttg gaaaaccgca
    39061 gtttgtttac tcatggaagt tagtgcctac ggataagacc ccggcaggta aacgggagga
    39121 tatttcactc cgggaaaagg agaaagaaat ccagaaagac cttgacgacg ggatagacac
    39181 catcggcaag aaaatgaccg tctgccagct ctacgccaag cagatcaggc accggggcaa
    39241 tgtgaagcac aacaccaaac tgggccggga acggctgatg cgtattctgg aacaagatcg
    39301 gctggggagc tgcccgattg acagtgtgaa gctgtccgac gcaaaggaat gggcgcttcg
    39361 gatgaaagaa aagggccttt cttataagac catcaataac gacaagcgtt ccctgaaagc
    39421 ggctttttac accgccatac aggacgattg catccgaaaa aacccgtttg acttccagct
    39481 tagtgacgtt ctggacgacg atacggaacc aaaggtgcct cttacaccag cacaggaaga
    39541 aagttttctg tcctttattc aaggtgataa ggtttaccaa aagcattatg acgcaatcgt
    39601 tattctgctg gggacagggc ttcgcatttc ggaactgtgc ggactgacgg acaaagattt
    39661 ggattttgaa aaccgggtaa tcatcgtgag ccaccagctt ttgagaaata ccggcgtcgg
    39721 ttactatatt gacgaaccca agacccaaag cggcgtcaga aaaatcccca tgaatgagga
    39781 agtgtatcag gcgttccagc gagtgataaa aaaccgcaaa ggtgcaaagc cgttcattat
    39841 tgacgggtat gccaatttcc tgtttctcaa acagaacggc tatcccatga cggcggtaga
    39901 ttatggcggg atgtttggca ggcttgtgaa gaagtacaac aagagccatg aagaagcgtt
    39961 gccgaaaacg acgacgcccc atgctatgcg tcatacattc tgcacccggt tagccaatgc
    40021 gggaatgaac cccaaagcct tgcagtacat tatgggacat tccaatatca ccatgacgct
    40081 gaactattat gcacacgcca cattcgattc cgcaagggca gaaatggaac ggctggcagc
    40141 ctgacacaag gccggtttta ctactccatt tactactttt gagggggaaa tggtgagagg
    40201 taataagaac atttgtaagt atctaacaat atcaaaaatg ccggaaaagc ccggaaacac
    40261 gggctatacc gacattcaag aacttctaaa aagataatct aaaatcccat ataatttttc
    40321 ctcaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat
    40381 cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc
    40441 atggattcag gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa
    40501 tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta
    40561 aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca
    40621 ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta
    40681 tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta
    40741 cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag
    40801 aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc
    40861 agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca
    40921 cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga
    40981 aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc
    41041 aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg
    41101 caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg
    41161 ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc
    41221 cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc
    41281 atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg
    41341 aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat
    41401 cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata
    41461 caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat
    41521 aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc
    41581 aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt
    41641 atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc
    41701 cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag
    41761 aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag
    41821 gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac
    41881 tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg actctagttg
    41941 caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa
    42001 gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta
    42061 agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt
    42121 cttccgcat
//
LOCUS       DADXTG010000010        42106 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.010, whole genome shotgun sequence.
ACCESSION   DADXTG010000010 DADXTG010000000
VERSION     DADXTG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.010"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            147..794
                     /locus_tag="IWI57_000424"
     CDS             147..794
                     /locus_tag="IWI57_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBC2448592.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            809..2335
                     /locus_tag="IWI57_000425"
     CDS             809..2335
                     /locus_tag="IWI57_000425"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBC2448593.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2384..5182)
                     /gene="ileS"
                     /locus_tag="IWI57_000426"
     CDS             complement(2384..5182)
                     /gene="ileS"
                     /locus_tag="IWI57_000426"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBC2448594.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5444..6151)
                     /locus_tag="IWI57_000427"
     CDS             complement(5444..6151)
                     /locus_tag="IWI57_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBC2448595.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6172..6954)
                     /locus_tag="IWI57_000428"
     CDS             complement(6172..6954)
                     /locus_tag="IWI57_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBC2448596.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7003..7290)
                     /locus_tag="IWI57_000429"
     CDS             complement(7003..7290)
                     /locus_tag="IWI57_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBC2448597.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7305..7904)
                     /locus_tag="IWI57_000430"
     CDS             complement(7305..7904)
                     /locus_tag="IWI57_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBC2448598.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7918..8595)
                     /locus_tag="IWI57_000431"
     CDS             complement(7918..8595)
                     /locus_tag="IWI57_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBC2448599.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8612..9853)
                     /gene="ftsZ"
                     /locus_tag="IWI57_000432"
     CDS             complement(8612..9853)
                     /gene="ftsZ"
                     /locus_tag="IWI57_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBC2448600.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9876..11201)
                     /gene="ftsA"
                     /locus_tag="IWI57_000433"
     CDS             complement(9876..11201)
                     /gene="ftsA"
                     /locus_tag="IWI57_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBC2448601.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11357..12577)
                     /locus_tag="IWI57_000434"
     CDS             complement(11357..12577)
                     /locus_tag="IWI57_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBC2448602.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12592..13680)
                     /gene="murG"
                     /locus_tag="IWI57_000435"
     CDS             complement(12592..13680)
                     /gene="murG"
                     /locus_tag="IWI57_000435"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBC2448603.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13706..15067)
                     /locus_tag="IWI57_000436"
     CDS             complement(13706..15067)
                     /locus_tag="IWI57_000436"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBC2448604.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15081..16043)
                     /locus_tag="IWI57_000437"
     CDS             complement(15081..16043)
                     /locus_tag="IWI57_000437"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBC2448605.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16069..18261)
                     /locus_tag="IWI57_000438"
     CDS             complement(16069..18261)
                     /locus_tag="IWI57_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBC2448606.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18262..18663)
                     /gene="ftsL"
                     /locus_tag="IWI57_000439"
     CDS             complement(18262..18663)
                     /gene="ftsL"
                     /locus_tag="IWI57_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBC2448607.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18668..19627)
                     /gene="rsmH"
                     /locus_tag="IWI57_000440"
     CDS             complement(18668..19627)
                     /gene="rsmH"
                     /locus_tag="IWI57_000440"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBC2448608.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19648..20079)
                     /gene="mraZ"
                     /locus_tag="IWI57_000441"
     CDS             complement(19648..20079)
                     /gene="mraZ"
                     /locus_tag="IWI57_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBC2448609.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20249..20617)
                     /locus_tag="IWI57_000442"
     CDS             complement(20249..20617)
                     /locus_tag="IWI57_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBC2448610.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20788..21744
                     /locus_tag="IWI57_000443"
     CDS             20788..21744
                     /locus_tag="IWI57_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBC2448611.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21817..21933
                     /locus_tag="IWI57_000444"
     CDS             21817..21933
                     /locus_tag="IWI57_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBC2448612.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21988..23670)
                     /gene="recN"
                     /locus_tag="IWI57_000445"
     CDS             complement(21988..23670)
                     /gene="recN"
                     /locus_tag="IWI57_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBC2448613.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23688..24137)
                     /locus_tag="IWI57_000446"
     CDS             complement(23688..24137)
                     /locus_tag="IWI57_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBC2448614.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24244..25062)
                     /locus_tag="IWI57_000447"
     CDS             complement(24244..25062)
                     /locus_tag="IWI57_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBC2448615.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25071..25961)
                     /locus_tag="IWI57_000448"
     CDS             complement(25071..25961)
                     /locus_tag="IWI57_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBC2448616.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25963..26187)
                     /locus_tag="IWI57_000449"
     CDS             complement(25963..26187)
                     /locus_tag="IWI57_000449"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBC2448617.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26192..27529)
                     /locus_tag="IWI57_000450"
     CDS             complement(26192..27529)
                     /locus_tag="IWI57_000450"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBC2448618.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27530..28384)
                     /locus_tag="IWI57_000451"
     CDS             complement(27530..28384)
                     /locus_tag="IWI57_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBC2448619.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28481..28930)
                     /gene="nusB"
                     /locus_tag="IWI57_000452"
     CDS             complement(28481..28930)
                     /gene="nusB"
                     /locus_tag="IWI57_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBC2448620.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28923..29348)
                     /locus_tag="IWI57_000453"
     CDS             complement(28923..29348)
                     /locus_tag="IWI57_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBC2448621.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29594..30658)
                     /locus_tag="IWI57_000454"
     CDS             complement(29594..30658)
                     /locus_tag="IWI57_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBC2448622.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30830..31075)
                     /locus_tag="IWI57_000455"
     CDS             complement(30830..31075)
                     /locus_tag="IWI57_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBC2448623.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31214..31588)
                     /locus_tag="IWI57_000456"
     CDS             complement(31214..31588)
                     /locus_tag="IWI57_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBC2448624.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31671..32585)
                     /locus_tag="IWI57_000457"
     CDS             complement(31671..32585)
                     /locus_tag="IWI57_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBC2448625.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32606..33409)
                     /locus_tag="IWI57_000458"
     CDS             complement(32606..33409)
                     /locus_tag="IWI57_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBC2448626.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33442..34431)
                     /gene="manX"
                     /locus_tag="IWI57_000459"
     CDS             complement(33442..34431)
                     /gene="manX"
                     /locus_tag="IWI57_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBC2448627.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34534..35025)
                     /locus_tag="IWI57_000460"
     CDS             complement(34534..35025)
                     /locus_tag="IWI57_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2448628.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35216..38023)
                     /locus_tag="IWI57_000461"
     CDS             complement(35216..38023)
                     /locus_tag="IWI57_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2448629.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38457..38750)
                     /gene="rpmA"
                     /locus_tag="IWI57_000462"
     CDS             complement(38457..38750)
                     /gene="rpmA"
                     /locus_tag="IWI57_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBC2448630.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38763..39107)
                     /locus_tag="IWI57_000463"
     CDS             complement(38763..39107)
                     /locus_tag="IWI57_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBC2448631.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39134..39442)
                     /gene="rplU"
                     /locus_tag="IWI57_000464"
     CDS             complement(39134..39442)
                     /gene="rplU"
                     /locus_tag="IWI57_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBC2448632.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39462..39538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39724..40365
                     /locus_tag="IWI57_000465"
     CDS             39724..40365
                     /locus_tag="IWI57_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBC2448633.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40427..42058)
                     /locus_tag="IWI57_000466"
     CDS             complement(40427..42058)
                     /locus_tag="IWI57_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBC2448634.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        11863 a         9170 c         7157 g        13916 t
ORIGIN      
        1 aagaatgagt cttctctatc atctttcgtt ttttgttgta aaaacgtcta aaaatccttt
       61 gttttttatt tagaaaaata gtcaattaac gttttttttg ttacacttac taatgatggt
      121 tttttaccat gaaaggatga tcgaatatga caccaaatcg agaagattat ttgaaaatca
      181 ttttagaact aggtggagat acaacgaaag tcaacaataa acaaatcgta tctagtctag
      241 atgtctcagc tgcatctgtc agtgaaatga tttctaaact tgtgaaagaa gagcttgtag
      301 aacattcccc ttatcaaggt gtacaattga ctgcaagcgg actgcaaaaa gctagcagtc
      361 tgattcgcaa acatcgctta tgggaagttt ttttagttga acatttagac tatagctgga
      421 atgaagtcca tgatgatgct gaagtattgg aacatgtgac ttcggaacat cttgcagatc
      481 atttagaaaa ctatttgaat catcctgaac attgtcctca tggaggatcg attcctaaaa
      541 aagaggaagt tgtccatgaa gaacgccgac aaacattgga gtcctatcct gtcggtacaa
      601 aagtgcgtat cgcacgagtg cttgatgaaa aagaattgtt ggattactta gtcactattg
      661 acctgaatat caacgaagaa tatgagatcg ttgacatcgc agcttatgaa ggaccgatca
      721 ctatccaaaa tgatagaaag aaaatccccg tcagctttaa ggcggccagc acgatttttg
      781 tcgataaaat ataaaggagt gtatccacat gtctaatgaa aaaaatgtat tgtttacgat
      841 attcggcggt acaggtgacc tagcccagcg taaattgtac ccgtctttgt tccgccttta
      901 cagaaaagga aatttaggcg agcattttgc tgtaatcgga accgctcgcc gtccctggag
      961 cgatgagcac taccgtgaag ttgtcaaaga aacaatccgt gcattgaatc cttcagatga
     1021 agaagcaact acctttgcaa gccattttta ctatcaatcc catgatgtaa atgattcaac
     1081 gcattatcag acattaaaag aattgtctga ccgattagat ggacactatc aattaaacgg
     1141 taaccgtgtc tattatttgg ctatggcacc tcaatttttt ggaacgatcg tttctcattt
     1201 gaaatcacaa aatattatga caaaaaatgg cttcaatcgt ttgatcatag aaaaaccgtt
     1261 cggttctgat taccagtctg cttttgaatt gaaccagcaa atcagagaag tattcccaga
     1321 acaagatatt ttcaggattg atcattattt aggaaaagaa atgatccaaa acatctcagc
     1381 tatccgtttt gccaataata tttttgaggc tcaatggaac aatcgctata tcgacaatat
     1441 ccaaattact tttggcgaaa acctcggtgt agaagatcgc gggggctact acgatcatag
     1501 tggcgcactt aaagacatgg tgcaaaatca tattttacag gttgtagcat tacttgcaat
     1561 ggaaccgcca gtagcatttt ccgaaaaaga aatccgtaca gaaaagatca aagcattgaa
     1621 agctatccgc atttataacg aagaagaagt gttagaaaac ttcgttcgcg ggcaatatgc
     1681 tcaaggtgaa ttggatggtg tacaattcaa gggctatcgc gaagaagaca aagtggatgc
     1741 tcaatcagag actgaaacat tcgtcgcagg caaattcact atcgataact tccgttggtc
     1801 aggtgttcct ttctatgtac gtacaggcaa acgtttgact gaaaaaggga ctcggatcaa
     1861 catcgtattc aaacaagttc ctgttaatgt attcaaaaca tctgtcgatg aaccatgtga
     1921 cgatacgaca cttccaccta atgttctaac gatctatatt cagccaactg aaggattttc
     1981 tttatccttg aatgggaaag aagtagggca aggatttgaa acggaaccaa tcaaattgga
     2041 atttagaaat agtgcagaaa tggttgaaaa tagcccagaa gcttacgaaa aattactatt
     2101 agatgcgttg aatggtgatg gaacgaactt ctctcattgg gaagaagtcg ctcaatcttg
     2161 gcatatcgtg gatgtcattc gaaaagcttg ggaccaaaca caaccggatt ttcccaacta
     2221 caaagctgga tcaatgggac ctaaagcagc tttcgaacta cttgaaaaag acggattcga
     2281 atggatctgg caaccagata actggtatcg tgaacgtgga aaattaaacg attgataaca
     2341 aaagggggtg tgacaaagaa catgtcacac ccccttttcc cttttacttt tcttcgaatc
     2401 cttcagctac agcttcagga tagaagtctt caacgatttt tgcacaacga ccgcatagat
     2461 gaggaagttt ttcatctact cctacatctt tacgaacttg gcgacagcga tcgcaagttt
     2521 ccccgtctgc tttttccact gtaatagcaa catcatcaaa taccatagcg ttatctggca
     2581 cttgttcatc tgctgccata acttcaaagt agtctggtga aacgatcaat aattgtgcaa
     2641 tatctgcatc caaagctttt aacatttcac gaacttgttg ttttggataa acagttagtt
     2701 ttgcttccaa agatttacca atcaatttgc tgtttcttgc ttcttccaat gcttttaaga
     2761 cattatcacg gaagtccatg aaagcagtcc aggtatccat caattcttct tggtttgcaa
     2821 actcttcata accaggaaac tcgcttaact gaacgtactc ctcttcttct ttcaggaatg
     2881 accagatttc ttctgctgta tgcggaatga taggagtcaa taattttgtc aaagctacag
     2941 ctgtttgata gaaaacagtt tgcatgcaac gacgttgata atcatcttct gcttcaatat
     3001 aaactacatc tttagcaaaa tccaggtaga atgacgatag atcaaccgtc aagaagttca
     3061 taactgtacg gtaaatatgc atgaagttat atttgtcgta cccttcttct cggatttctt
     3121 tgattacttg gttcaatcga acggtcatat atttgtctac agaacgtaaa tcattgtacg
     3181 aaacagtatc ttttttagga tcgaaatctt ctgtattcgc taataagaaa cgcatcgtat
     3241 ttctgatttt acgatatact tctgccactt gacttaagat atccatagaa acacgaacat
     3301 ctgcttcata atctacacta gatacccata agcgaaggat atctgcacca aattgtttga
     3361 tcactttttc tggtgcaatg atgtttccta aagatttact catcttacgg ccttcaccat
     3421 ctaaagcaaa tccttgagaa ataactgatt tgtatggtgc tacaccattg atagccacac
     3481 tagtcgtgat acttgagttg aaccagccac gatattggtc tgatccttct aaatacatat
     3541 ctgctgggaa tgataattct ggtcgctgac gtaaaacagc ttcatgagaa gaaccagaat
     3601 caaaccagac atccatgata tctttttctt ttgtaaattc gccattagga gaacctgggt
     3661 gggtaaatcc ttcaggaaga agttctttcg cttctttttc aaaccagata attgatccat
     3721 gttcagcaaa taattttgcc acatgttcga ttgtttctgg tgtgatgatt gcttctccat
     3781 tttcagcata gaaaatcgga agtggtactc cccatgcacg ctgacgggaa attacccaat
     3841 cgccgcggtc acggatcata ttatacaaac gtgttttacc ccatgggata acccactcta
     3901 ctttttctac ttcatccaaa atattctgac ggaatttatc gatagatgca aaccattgtg
     3961 gagttgcccg atagatcaca ggctttttcg tccgccaatc gtgaggataa ctatgagtaa
     4021 agaaatctag cttcagcaaa gctccttttt cttttaatag atcagtgatc ataggatttg
     4081 ctttgtcata aaagatacct tcaaatccag gtgcttcttc tgtaaacaat ccttttgagt
     4141 ctacaggtga cactacagat agtttatatt tgctaccagc gatatagtca tcttccccat
     4201 gtcctggagc agtatggact aatccagtac cagcgtctaa agtaacatga tcgcctaaaa
     4261 tcacaagtga ttcacgttca tagaatggat gacttgcagt catatattcc atttcttgtc
     4321 cagcaaattc ttttagaact gttacatttt cccaaccgat ttctgattgg actgtttcta
     4381 gcaaatcttt cgctacgaca aacttacggc catcggcttc caccaataca tacgtaaatt
     4441 ttggatttac agcaatacct tggtttgctg gcaatgtcca tggtgttgtc gtccaaatga
     4501 caaaagctgt atcttcatca agaatatctt tgccgtcttt gacattgaaa gctacaaaaa
     4561 tagatggaga ttttacatct ttatattcaa tttcagcttc cgctaaagaa gactcacttg
     4621 acggagacca gtaaattggt ttcaatcctt tgtaaatata gcctttttca gccattttac
     4681 caaatacgcg tacttgtgca gcttcataag aaggatctaa tgtaacataa ggatgttccc
     4741 aatctcctgc aactcctagg cgtttgaaat ctgctcgttg cttatctact tgggaaagtg
     4801 catattcacg acatttttcc aagtattctg ccatggacat ttctttacgt ttcacgcctt
     4861 tattagccaa tacttgttca atcggcaatc catgtgtatc ccatcctgga acatatggtg
     4921 cacgatagcc agacattgat tttgaacgaa tgataatgtc tttactgatt ttattcaatg
     4981 aatgtcctaa atggatatta ccattggcat atggaggtcc atcatgaaga acaaagctag
     5041 gttttccttc gttcaactct tgtctttttt ggtagattcg attttcttcc cattcttttt
     5101 gccactcgcc ttctcggttt ggcaaattac cacgcatagg gaattttgtt tttcctaatt
     5161 gcaaggtttc tttcattttc attcaaccat acactccttt atttgttttc cggacacaaa
     5221 aaagcatgtt cagccgcaag ggacgaacat gccgtggtac cacccaaatt tagttgcatc
     5281 tatctagtgc aaactctctg tgaccaataa cgaggtcagc cgctgcttct tactctaatt
     5341 tcggaaaagc aggtctcaag tagaggatct ttcaccatgc agtgtcttac cgaacttcca
     5401 ctctcatcgg ctcgctttaa gaataacaaa gtgcttttct gttctactcg ttttcatttt
     5461 caccaggcac gatcgagata tcaatagcct gtgtatcaaa tgccgtcata ggtgcttcta
     5521 tgactcctgt gtctgagttt acatcagtat cattttcatt gtcaagctgt tttgctaaaa
     5581 cttctttgat cacttcatgg ctgtcagtca cgtagctgga gaatggttgt aaaatttcat
     5641 cccattccgg acttttcact tgttctaatt gagactcaag catcaagctg atacgttgat
     5701 ggaatactcg tgtttttttc ttaagatctt cagtttctgt cgcaagctgt cttgcttttt
     5761 ctgttgcatc tgtcaagatt tttcttgctt tttcttctgc atcagttgtt aattgatgtg
     5821 cacgtttttc agcgttcgca actaactcat ctgctttgtt ttgtgcagaa gtaacgatca
     5881 cttcggattc cttgcttgca cttgttttta ctttatcagc cgtatcttga gctacgatga
     5941 ttgattggtt caaagcatct tttaattcat tgaagtactc aagtttttct tcagaatgtt
     6001 tgactgcttt ttctaattct cgatttcttt gagtcaattc ctcatagtca cgaacaacta
     6061 aatctaagaa atcatctact tcgtcttgat tatatccccg catttttgtc gggaatgtct
     6121 tattttggat atctaatgga gttaaagcca tgagtaaacc acctttttat tttatttacg
     6181 taatacaccg aacagtaccc tgtacttttc ttttttcgtc ttcccctcaa gttcttggat
     6241 ctggactctg ccataacctc tgactgaaat aatatctaat agttcaagta caaaatcagg
     6301 tcttgtattt tctgtccagt tcacttttac tttaccagct tcgatcagct gcttggaccg
     6361 ctgtctggaa atattataaa tctcagagat cacactgtct agtcgcatgg aactaactgt
     6421 tcctctttct tgtgtccagc tatctttcgg cataatcatt tctgtgtatt tgattggttc
     6481 caagcgtaca gcaacctttc caatctttgt tacttgtgca gcaacaaaat gatccacctc
     6541 atttgcaata aatacttgcc agcgatcacc gtccgagatg atatcgccaa agtaagagcg
     6601 tttgatcccc gttcccacta aagttccaag gattttccca tgacttaggc tggcaaattt
     6661 gctaggataa tggatttcat acaatgctgt ttcaaaatct tcctctacag gttcgtaata
     6721 atcggggaaa atcaaacatc tttttctttc tgcctgttca tatccgccat aaaacatgaa
     6781 acgcaaatca ctgctttgtc gaatcagtgt ttctaaaata taggattgtc ttgggtcaag
     6841 aaattcggtc agatagggtg catattgtcc ttgaacctgt tcaatccaat cgcctaccga
     6901 atcgataaat gtacgttcat ctgcccgaaa gtgctggtat acatttgcat ccaaaatttt
     6961 cccctcctat ctattctgag aataaagatt ttgttgcttc aatcaataaa ctaaatggac
     7021 agttatccat ataacgatct gtgctaaacc ttgtacggct aagttcaaga tcaggattgc
     7081 ccctaaaatt gtgaaatcca agggtccaat cctcaaaggc agacgatcga acaaactgat
     7141 atacggttcg cagatcttag ctaataatcg tcctagccct gattgatacc cgcctggaaa
     7201 ccaagagagc aatgcatata ccatcaagat aatgctatag aaataggctg cccgttgaag
     7261 taaatgaagt aatagtgcaa tagtataaat cgtagttcct cctcttacag atcaaacaaa
     7321 ttcgcatctg caaaagattg agcggttgaa ctgtcaatct ccatcccagt cggtgtacaa
     7381 agaaaaatct catctccaac acgtttgata tctccatcta gtgcataaac tgttcctgtt
     7441 agaaaatcaa cgattcttct cgcttgtttt tcttctacta aatgaaaatt cactaaggtt
     7501 gcttctccag caatgattcg tttagcaatc gccatagctt cagaataagc tcttggttcc
     7561 attacagtga tttttccagc aagatttgct ttaggttgtg tagaggttct tgaagctgtt
     7621 ctttctttcg ctacagtttt tgtaggtgat tgatgtagtt caattacttt tttcttttca
     7681 gccggctgaa tactttcatg cgttacagga cgtgaagtcg ccgttttttg tttttcatga
     7741 gtattcgcag cagatacaga atgagttgat tgattctgtg ttgtattttt tttagttggc
     7801 tgtacagcct tttttgttgt ttcaggataa tcttggtatt cgtactcttc ctcatcagct
     7861 aatccgaaaa aattagcaat tgctcctctg tttaaaagcg gcatttttgt tcctccttta
     7921 ttcgtctttg aatagcgctg ttcctacacg gataaatgtt gcaccttctt caatcgcaat
     7981 ttgaaagtct tgactcatcc ccatacttaa ttcactacat ggtgcatggg cataaccttt
     8041 tgcttgaaca agatcacgca attctttcag cgtaccaaac actttatgga tttgctcatc
     8101 agaagcttct ttaggcgcca tagtcatcaa cccaacaacc ttgatttttg tgcaggcagc
     8161 taaagattcc acaaacggta aaagctcatc tggtgaaatt ccatgttttg attcctcacc
     8221 agaaacattt acttcaataa aacaggataa ttctttctct aaccgcttct ggatttcttc
     8281 tgctaatctt aaactgtcca acgcatgaaa gtaatcaatt tcatttacaa tcaattttac
     8341 ctttctgcgc tgtaaattac caattaaatg ccactttatt tcaggaaaat caaataaatc
     8401 ttgtttcttt tctaataatt tatctactcg gttttctgca caattcataa cgccagctct
     8461 aaccaattct tcagcttgag cggattcaac agattttgtt acagctacta atgtaacttc
     8521 tttcggatca cgatgaacaa gagcacacga atcctgaatt tcttgcttga ttttctgcaa
     8581 gttgtcagca atcatgtgct cttcctctcc tttatttttt acgacggaaa aacggtggcg
     8641 tatttaattc atcatcgcca tgagattttg tttcttcgcg gttgaatgta tcaaattctt
     8701 ttttttcgat attgtcaaaa ttggaatcat caactctagg acgaacattt tgttcacgtc
     8761 tgatatccca atcgccaaaa ctgttttctt cttctgaaga agttggttta gcttgatcca
     8821 tatctaatac agtcttttga gcaggtgatt gcatttgtgc ttgtctagct ggtcggctag
     8881 acttgcgttc cttttttgat tcatcgatac ctgttgcgat gaccgtcaca cggatttcat
     8941 cgcccatttc ttcattgata gatgtaccta gaatgatatt tacatcgcca gttgctgcat
     9001 ttgctacgat atctgaagca tcttgtgctt cgaacaatgt catatccaaa ccgcctgtga
     9061 tgttaagaag tacttgttca gctccatcaa tagatgtttc aagcaatgga gaggagattg
     9121 cttttttcgt tgcttctact acgcgatctt ctccactagc cacaccgatc cccatcaatg
     9181 ctgtaccttg gttttccatc actgttttca cgtcagcaaa gtcaaggtta acgtaacctg
     9241 gcgctgtgat caaatcagag ataccttgta caccttgtcg taaaacatta tctgcttcac
     9301 ggaaagcttc aagcatcggt gtctttttat ctacaacttc taataaacgg ttatttgaga
     9361 taattaataa tgtatccaca ttttctttca aacgagcgat accttctgca gcaaaacggc
     9421 cacgtttcgg tccttcaaaa gtgaatggac gtgtcacgac accaacagtc aatgcgccca
     9481 attctttcgc gatacctgca acgattggtg cagcacctgt accggttcct ccacccattc
     9541 ctgcagtgat gaaaatcata tctgcaccgt ctaaagcttc gcgtaaggat tgttcacttt
     9601 cttctgccgc tttttgtcca acttctggct gagaaccagc acctaaacca cgagtatatt
     9661 tggggcctaa ttggataact gtttcagctt ttgaattttt taatgcttgc acgtctgtgt
     9721 tggctgtaat gaattcaaca cctttaacgt tttcttcaat cattcggttt acagcatttc
     9781 caccgccacc accgactccg atgactttga ttactgcgcc gtcattgata ttattatcaa
     9841 ttgagaattc catatcttat tcctcccagg atgaattaat caaaaatatt tgtaaagaaa
     9901 cccttgattt tattttttac attttctccg gactcttttg gttccggctc atcgtagatt
     9961 gtttcttcag gtgcttcata gctctcatat gtgtttgttg tttcttgttc aactaccatt
    10021 tgacgagctg ctgaggtttc acccgtaaca gcaatttttg ccaattgata cacttcactc
    10081 aagtttgctg aataatccac gatacttatt acatttgtaa ataccgggtt tctcagcccc
    10141 atgtgatttg gaacatacaa ttttacattt acgccgaaga tttcttgtgc caagtccaca
    10201 acaccaggaa gactagcagc tccgcctgtc aatacgacgc cacctggcaa ctctaatgct
    10261 tcgatttggt ccaatgcatc ttttgctttg ttgaaaatct gttccattcg agctgaaata
    10321 atttctgaca agtagcgttc atcgactttc actggttctg actgaccaat cacatctact
    10381 ggaaattctt catctggtga tgtccgttct gggtatgcat ccccgtagtt tattttcaac
    10441 gcttctgcat tgttaaatga cgtatttaat acaatagaga tatctttagt gacaaattcg
    10501 ccaccttctt ggtcaagatt ggtaaacttc aattgcttat catgcataac agccgttgtt
    10561 gtttggccgc cacccatatc aatgacgatc gtaccaaaat ctttttctcc gtcagaaaga
    10621 attgattcag tcagcgctaa aggagtaatc accatttcgt tgacgattaa tccggcattt
    10681 tccacgcatt tacgtatgtt atggatgatc gttttaggtc ctgtaaacaa cagaccgtac
    10741 atttctaatc ttacgccgat cattccacga ggatctttga ttccctcgaa cccatcgaca
    10801 gtaaaatctt gtggcaatat tgacacgatt tgacgttcag gaggtattga acgaacaagg
    10861 gcagctgaag ccacatttct gacatcttca tcggtgattt cctttgaatc tccgttaact
    10921 gcaatcattc cctggcaatt ttctacttca agcatgtttg ctggcaaacc gacacttacg
    10981 cctttgatct ggatacctgc tttttcttct gcttgtcgta ctgctcgttg tatcgcttgg
    11041 accgttttat cgatatcaac gataatgcct cggttaatcc cttctgattt tgcgtttcct
    11101 actccaataa tattcatttg gctgtcgata tattcagcca cgacaacttt aacagatgtc
    11161 gttccaatat caaggcctac atacattcct gtttttgcca tgaatgggat tccctcctac
    11221 ttactattcc atatacaagc gaacaactag cgtccgtttt aaaatatact tattaataga
    11281 tatattcact gcttccaatt ctaccataaa ttactggttt ttagaaagta cattcttact
    11341 ttttaaagtt gttttatcaa ttttcattta aacttgacgg agttgtactt ggattatctt
    11401 ctatttgact tgtttcactt gaatcatttg aatcagatga tccgtttgtg ctagacgatc
    11461 caggtgtttg tgctttttgc gtttctgcct cttcacttga ttcgtttgtt tgatccgcat
    11521 tttcttcact ttcagttgtt tcttccgatt ggcccgattc ttccgtttct tgagtgctac
    11581 cgtaaggatg cgagaagata ccaacttcca tgtcgatcac acctttttca tccatatctt
    11641 tggctacttg cgaataatat ttcatttggt tcgcaagatt attgatattt acaataactt
    11701 gattgccgtc attcatattc aattggatca gatttttatt gcttgctctt ggcgtgtact
    11761 tgatttcaga aattgctttt tgtaattctg atgataactg attgtattgc tttgttaatt
    11821 ctttgatttt tgcagagtct ttgaactctt ccaagatagg gagatttttt gtaggatttg
    11881 ctactttttc atctaatact gttccatttt caatcactgg gtagtattga ttgttttttg
    11941 cgatcaaagc aatctcttta tattctgtta catccaaatc gaattcattc atccctttga
    12001 aatgtatatt tgctgtttcg attcttggct gtgctttttt aaggcgatcg atataagtag
    12061 atcgatgaaa atactgtccc caaacatttt ctcctaatga caattttgta tcagagatga
    12121 tttcttggct gttgactgtc ttattgcctg atactgcaac tgcttgtaat ttgctataag
    12181 gagaaacata atagatcaaa aaaatcagcg gtatacccaa tacagtgatg atcaaagcta
    12241 accgacgaaa tagtactttg tgccgttgac tttttaaatt gggcagccgg ttcaaaaaag
    12301 aaccgttata tggtccaccg tttttctttt gcttgatctc ttcaaaaaat tcgatttttt
    12361 tctcggactg gctttcttct tgctgttcct cttgaaccgt tttttcttct tcagaatctg
    12421 aaaggggtgt atctaatgtt atgtcttctt tttttggctc ttggtttttc atctcttgtt
    12481 ctgccagata ttttcggtta gccttttgcc aaggagtcaa attgttttca tcagaagacg
    12541 gatgagacaa tttttttggt tcttttttct tactcactaa gaccaccgcc tttatttttt
    12601 gattatttgt tttactaagt cataaagacg ttttgaagca tcagggattc cttgttcttt
    12661 agaagctcgg cacatttgct tttgcaattc ttcatcaccc atgatttcgt tgactgtctg
    12721 acttagtgat tcacctgtta gttcattatc tgcaatcatt tttgctgctc ctgcgtgaac
    12781 aagactcatt gcatttttag tctgatgatc attcgtaaca tatgggctgg gaaccaacac
    12841 tgccggtaac cccagagctg tgaattctgc gatcgaagtt gcaccagcac gccccaccag
    12901 cagatcactg gaagccatga cctctgccat atttttgata taaggctgga cactaatatt
    12961 ttgtatttca ggatttattt tatttaggat ctcttcataa tatctatctc ctgaagcata
    13021 caaaacttgg taagaagaag gaatatattc acttaaaaaa actgatactg cttgattgat
    13081 tttaagtgct ccttgactac cgccaaaaat caagactgtt ttcttttctg gatccaaatc
    13141 aaatgttttc aaaatatctg attttttagt atctgctact tcttgtgctc gaggattacc
    13201 tacaagaact gctttgtttt caggaaaaaa ggaagccgca tctttaaatg aaagagcgat
    13261 ccgatccaca tatcggctta aaaatttatt ggtaacacct ggtacactgt tttgttcatg
    13321 gataactgtt ggaataccca ttttggctgc agcgtacact accgctccgg aaacatatcc
    13381 tccagtaccg ataacgatat ctggttgaaa atcactgagg attttttttg cttgacggat
    13441 actcttaaga aacaatcgga ccgtcttgat gttatgcatg gaaatttttc gtttaaatcc
    13501 ttgaatctcc aaggtatgga aaggcattcc tgtatcaggg acgattttgt tttctagacc
    13561 tcgttttgct cccacataca tgaattcagc attcggttct tgagtcttta cataattgac
    13621 aaaggcaaga gctggataaa tatgtccccc tgttcctccg cctgtaacca aaattttcat
    13681 tttttatcgc tcgcttctac tttttctatt ttaatcggtt cacagcttcc atgaatctgt
    13741 ttcctctagt ttcaaaattt gggtattgat cccagctggc attcgctggt gataataaaa
    13801 ctacatcacc atctgtactg ttttctaatg ctaaaggaac tgcttgttct acagaatcgg
    13861 ctgttactat tgtctcgatg cctgcttttt ttccagcatc catcaacttt tcttttgttt
    13921 ggccaaatac gaccattgct ttgattcctt ttaatgaagg aattaattca tcaaatgtat
    13981 ttcctctgtc taatccgcca gccaataaca cgaccttaga cgtttcaaat ccacttagag
    14041 ccattttagt cgctaaaata ttggttgctt ttgaatcatt ataaaatttt ctgccttgga
    14101 tttctcctac atattgcgta cgatgtggta cgccatggaa aaaatgcaac gtttctcgta
    14161 tagcttcatt tgatatacca tacaattttg ctacagaaat agcagctagt gcattttcta
    14221 cattatgctt acctggtacg ccaagttcag atatttccat tattttttct tttttatagt
    14281 atattgagta atcgtcagca tacacaccgt cttccaacac ttcttttgtc gagaaaggca
    14341 gtacgcgtgc ttttgttctt tgcgctaatt cttgtagttc tgactgattc cagttaagga
    14401 tcagataatc tttttctgtc atgttttttt gaaggttcca tttagctttg acatattctt
    14461 ttcttgtacc atgataatca atatgtgctt catagatatt ggtaatcacc gctatatgag
    14521 ggcggaaatc tgtgattccc atgagttgga agctagacaa ttccatcaca attttatctt
    14581 ctgattttgc ttcttgcgct acaccacttg ctggataccc aatattaccc gctaaacgtg
    14641 caattccttg atctgctcct gcattcagta gtagtcctgt catcgttgtt gtcgttgttt
    14701 ttccgttcgt tcctgtaatc ccaataattg ggcattccgc tacttcgtaa gctagctcaa
    14761 cttccgtaat taccggaata cccatttctt gggcttttgc tactaaagga tgactgtagg
    14821 gaatccctgg atttttgacc atcaatgaaa attcttcatc caacaattcg attggatgac
    14881 tacctgttat tacttttatc cctaaagaaa gcagctcttg tgcttccgga ttttcgtcaa
    14941 aaggttttcc atcattaacg gtgacaagtg cgcccaattc atgtaataat ttggccgcac
    15001 ttactccgct ttttgcgagt cctaagacaa gcactttctt attttcatat gttgatattt
    15061 ttttcatttc ccgtcatctc ctaccaaacg atccataatg tgatcaacga agtcacaatg
    15121 ctgatcaacc aaaaaactgt atcaatcttc cactccgacc atccgcacat ttcaaagtga
    15181 tgatgaattg gagacatttt aaaaattcgt tttcctgtaa gcttaaagga agtaacttga
    15241 agcatcacac ttgctgtttc cataacatag atcaaaccaa tcaaaagcaa tgtccattct
    15301 tggtttagca ttatagaaat tgccgctaaa agtccgccca acgctaatga accgacatct
    15361 cccataaaaa tcttggcagg ttttctgttg tagacgaaga aacccagtaa acctcctaaa
    15421 acacttaagc aaatgattaa tacatcatac tgatgctgat gccaagcaat tagaccgtaa
    15481 gctgcaaaag agatacttcc taagccagca actaatccat cgatgccatc tgtcagattt
    15541 actgcatttg aaaatccaac aagccagaag agagcgaaaa taccataaat gattcctaaa
    15601 ggtagatcta gtccgaagaa attcaataca cctggataac cttcagaacg gtaaacaaga
    15661 taaaagatga ttccccctag aatctgtcca atcaatttct gcttcgagtt cagtcccatg
    15721 tttcttttct tgaatatttt tataaaatca tcaagaaagc cgattactcc gtataacgct
    15781 aatacaaaaa gcaagatgaa aagagtagga gttagctgtt tttgccaagc tccaacccaa
    15841 attcctgtca aaatagagcc gatcagaaac actaagccgc ccattgtagg agttccggct
    15901 ttgctattgt gccactttgg tccctcctca cgaatcgcct gaccttgctt tttcatttga
    15961 aaataaccaa taaatagcgg cattgcggca attgtcattg cacaactgct gacaatggga
    16021 atcaatgctt gtgtccattc catgctcttt tctctcctat ttctcttctt attcttctaa
    16081 agtaaaacta agtttgtctt cagtgatttt ttcatatgga gcaagttctt gtttgacaca
    16141 ataaccatct ccatcaaaac taacttcaat accgaggatt ttccccagct tcatgatatc
    16201 tgcttttgac catcctgtta cgtcaggcat cagcttatca ccaccagtat ataggatcag
    16261 tttttctcca gagatcaact gatcaccgtt ggcagtagat tgtttttgta cttttttacc
    16321 attaccgatc actacaggtt gcagtccact tttttgagca tcagctgctg caacgtccgc
    16381 ttcaagatta cgataatcgg ctacggatac tttttctgtt tttgtttctg tatctgaatc
    16441 ttcttcagtt tctttaaaat ccattgcccg tttcatcaat ggatttgcga ttttagccaa
    16501 tgccattcga tcatatgtct tagggtgttt cattgttaga tacaagacat agtctggatc
    16561 ttcagaagga accatctcca caatcgaata caggtaggct gtatctcctg tttgatatcc
    16621 ccctgtatca gaagcgattt gggctgttcc gttttttgcc gatacattgt atccaggtac
    16681 actatataca ccataagcac taccgtagtt ttcgctttcg acaacatctc tcatatattc
    16741 acggactttt tctgttgttt cttttgaaaa aggttgccct agaacttctg tttgagttgt
    16801 tcgctcttct ccagtttggg gatccaccac tttgctgata tatcgaggtt gaatcattgt
    16861 accgttattc gcaatagaag tgaaagcttt catcatttgg aaattcgtca cacctaccgc
    16921 ttgtccataa gcactcattg ctcggtcgac aatatttaat gtaggtaaag caccgtttac
    16981 ttcatcatct aatccagaat gagtactttg tccaaatccc agtttttgta aatagttata
    17041 ccatcgtccg cctaatcgct gttccagtat gaccatcccg acgttacttg accatgaaag
    17101 agcttgacgc atggtcagca cacctttttc accaaagtca tggtcattga tcgtagcatc
    17161 ttctacttgg atttttccag attggaaggt ttcattctcg ttgaattctc cttcttcgat
    17221 agcagctgca gtagtgaaaa ctttcatggt tgatcctggt tcataactat cttgtaccaa
    17281 gaaattacgc cagatagcat cttttccagt caatccttcc attgtttcag gattgaaagt
    17341 aggtcgttgt cccattgcca agatttctcc ggtttttgct ttcatcagga ctgctgtcaa
    17401 ttcttctggc tgatattctt catttacttg atccatcagt gtttctaaat aactttgtag
    17461 tctgctgtct aaagtcgtgt agatatcttg tccgtcgaca gctttttctt cttctgccac
    17521 tgtacctggc agaggatttt gaaaattatc tttttgataa ataatcttac catctttacc
    17581 gctcaagata tcattgtaag cagactccaa acctagttta ccaactaagc cattctcatc
    17641 tttgtctgga actgcgtatc caataaaatg agaagaaaag acgccatttg gatacatccg
    17701 agcctgatgg tcgacaaaat aaagaccagc gacaccttct tttttcatgt ccgcttcgat
    17761 gttttgcttg gtttcttggg agatattttt cccatagcta ccaaattcaa cttgatacag
    17821 ctcttttttt gcgccgtcct ctaaaatatt cagtgcatct tttttgtcta tagaaagatt
    17881 cttatgcagg atttctgcta ttttatcaaa attcttttct tcaacataca gttttttatc
    17941 cccagaggta tatgtttttg agagtatcgc tttaattgaa taagaggagg catcttctgc
    18001 gagcgccaca ccgttacgat cataaatcgt tcctcttttt gcttttacta cttcacttcc
    18061 ttggtacaac tgttttgttt tttcagctaa tgaggtacca gcaacatgac ctccaataac
    18121 gatatacgag aaacggacgg caaataaaaa gaacaatcca atactcgtag caaataaaat
    18181 gatacctact tttttgcgat tgttcatcgg atttagattt ttcttttcca taaaccgccg
    18241 aattttattt ttaagactca tttacttcac tttccttaaa ttgtcgtcat taatagacaa
    18301 tcctttttct tctgcgattt gtttaatccg ttcagtacga gaaagttcgt ttttttcttg
    18361 ttctaaactc gttttttctt gctttttatc attgatttct gtttgtattg acgtgatttc
    18421 tttttcagct ccgctgatat ttgtgcgcag cataactgtt aaaacacata aagcaacaat
    18481 agcaatcgcc acgcttgcca cagcaatttt ttccaaagca gaaatctgct ttaatcgttt
    18541 tgccggtgat tgtggaagaa tgatttcttg attcgaagaa gaatcaggca tttgttgttc
    18601 cggctgatcc accaattcag gtaaatcata ttcgtaatct tgcattttct ttaattctgc
    18661 catcattcta ctcctctttc attcttattt tttctgcaat acgtaacttt gcactgcgtg
    18721 atcgattgtt ttctgttagc tcttcctctg aaggaaggat tggtttacga gtaatgactt
    18781 tcagttctgg ttggaattcg tcaggtatga caggaagtcc cggtggtaga tcttgtaccg
    18841 tactatattc tttaaacata cttttgacaa ttctgtcttc caatgaatgg aatgtgatta
    18901 cgctgattct tccattgact ttcaataacc ggattgcctg ttctaaagag gcctcttcgg
    18961 caccgagttc gtcatttact gcaatccgca cagcttgaaa aattcttttt gcaggatgcc
    19021 cgccttttct tcttgcggga gctgggatga cttgtttaat gatttcgaca agctcaccag
    19081 ttgtttggat tggttttgtt tttcgtactc gttcgatttc acgagcaatt tgtttggaaa
    19141 atttctcctc gccatagcgg aagaaaatct ttactaattc atggtaggaa tattcattaa
    19201 ttacgtgata agcggataaa ggagctgact gatccatgcg catatccaac ggtgcatctt
    19261 gatgataact aaatccacgt tctgcttcat ctagctgagg agaggaaacc cctagatcat
    19321 acaaaatgcc gtctacctta tctacatgct cactcaagcg ttcttctaat tctctgaagt
    19381 tcgctttgat aaatgtgacc ataccttgtt cgacataaga agctaaacgc agcttggcgt
    19441 gatcgattgc tttttgatct tggtcaaacg catacagatg ccctttagaa cttagttggc
    19501 ttaataaata ttcactatgt ccggcaccgc cgagtgtaca gtctacataa acgccatccg
    19561 gtttgatgtt caatccatct accgtttctt tcaacatgac tgtatagtgt tgaaatgttt
    19621 cagtcattag tcagcctctt cctattctta tagtccgaaa tcaatcatac tttcagcaat
    19681 ttcgtcaaag ttctcttctg ctgctgcaga aaaatcttgc catcgtgtct catcccaaat
    19741 ttcaattcgg ttagatacac cgatgactac gcaacctttt gtcaaagctg cataatttct
    19801 taacgtgctg ggtatattga ttcgtccttg tttatcaagt tcgcattcag tggcagccga
    19861 atagaagaat cgcacaaaag tacgtgcgtc ttttttagcc aacggcattt cgttcagttt
    19921 agcttccaga ttttcccatt cgctcatcgg atagccgaaa agacagccat ccaatccgcg
    19981 tgtcagaaca aacttttcgc caagttcttc acgaagtttt gagggcacga ttaaacgtcc
    20041 ttttgcatca atattatgtt ggaattcacc catcaacatt gaaagccccc cattcctcca
    20101 ctaactaaaa ttagtctacc acaatccccc actttctacc accttttctg ggaaatccgg
    20161 gaacaacttt ttttattcat aaacattttt ttaaaaatca agctgcaaaa agccttttaa
    20221 aagacttttt acagcttttc tatttttttc ataggtaaga aagtatgttc acaatgatca
    20281 agacagcgta caagacgatt gtcaacagaa aaaccaaacg ccaagtcatc ttgaaataac
    20341 gcggataaat aatttcttca taataatatg cttggaaaac agcaatacaa attcccatta
    20401 gcaaaacaga aataaagaaa aacggtaaga tagaaattgt ccctgtattt ctagacaatg
    20461 catgcatacc gccccacaaa aaaggaatcg ccaaatcagg ggcttttatt ttaaaacgac
    20521 gatttaacga aaataatttt acaagaaatt ggcaaccaaa caaaacgatt accgggtaaa
    20581 tataccaaaa caagataagt ggtgtgaatg tgcccatgaa cgtaaactcc ttttttatcg
    20641 ttgcttcaag tatactgtat atactttaca caactgtttc attttattta aatttatgca
    20701 gttatattct atatcttaca aacacattcc atgtattcca attccctatc cgttataata
    20761 aaagaaacag gcaacaggag gaaaatcatg atcaaatatt ttacattaga taatgagaaa
    20821 ctgcgtaata ccggaaagga aactgaccaa acgatctggt attgtgtaga acgtcctaat
    20881 caagaagaga ttcatcagct gacacatcaa tttgaaattc cgtcagatta catcacttcc
    20941 atattggacg atgcagaaaa ttcccgtaca gaagaattca atcaatcaaa gttgacaaag
    21001 ccagcgttat tattgttaca atatcctttt gctaaaacaa gtcctagcgg ttattttcaa
    21061 gtggatacat acccgctctc tattattttg acgcccaaaa agaagcttat cacaattacg
    21121 aatcatgaac cactgttttt aaagaaagtc tttagccaga cctttccaaa aagtgatatt
    21181 tcgcctaatc tcaatatctt ctttcaattc atttggcagg tcacactctc ttacaacagt
    21241 tatttagcta cgcttcaatc tcaatgcgat actttggaag ccaaacttca agtatccact
    21301 gaaaacaaac aactttatca aatcatggat atccaaaaaa gcctcgttta tttcagagaa
    21361 gcgacttctg ctaatttaga tgcattgaaa gaactagcgc aaaataaact tttccaagaa
    21421 aatcatgctt tgaaaaatca tttacgtgat gtattggtgg aaacagagca ggcaatgact
    21481 acttctcgca ttcaattaaa actagtagaa caaatgaatc agactttttc agcaatcgta
    21541 tcgaataatc tgaataatat tatgaaaatt ttaacttctt taacgattat tttaactata
    21601 ccgacaatcg tcggcagtat ctatggaatg aacattaagt tacctattgc tgaaagggat
    21661 gatgccttta tatggctagt tctattaaca ttatttattt gtatcgtaac tacctattat
    21721 ttaaaaaaag ggaagttttt ataaaaaagc cctcttgtgg aaatggaaaa aagattgtac
    21781 acttgaatca actaattaat gacgaggttg aaaacaatgc gtgaaaaaaa acaaacaagt
    21841 acttttgcta aagtaacaaa atttgtcatt tggacaatgc tgattttaac gattggatct
    21901 gttgtattga ctgctgtaat gagtgtcatg taaaaataaa atatagctat cagctgctga
    21961 aaaggacctg aaccactgcg aggtggctca ggtccttttt gcgagtttca atagttcttt
    22021 tgcatgttcg agtgtgagtt ttgtaatctc acttccagcc agcatacgtg caatttcttc
    22081 ttctctttct tttgttttca actctgaaac agaagtttgc gtacgtccat caatcactgc
    22141 tttcacaata taatattgat agtcggcgac tgcagcaact tgaggcagat gagtgatgca
    22201 caaaacttga gagtatttcg atatttgaga aattttatct gcgatggcct gtgcaactct
    22261 tccgctcaca cctgtatcca cttcatcaaa aataatactt gtcacacctt gctcagaaga
    22321 aaaaatagtt ttcaaagcaa gcaacatccg agacagttcg ccaccagaag cgactttcac
    22381 caaaggcttc agtggctctc ctggattggt cgtaatgtaa aattcgattt ggtcaaatcc
    22441 atcatgattc aattgtctat tttcttcttt taagaaacgt acctcaaact cagtgttttc
    22501 catatacaga cttttcaatt catgcagaat ggatttttcc aattcttttg cgattttttt
    22561 acgcgcttca tgaagatctg cggcttgttg ctgaagtaga agctcttttt gctctagttc
    22621 ctctttcatt ttgtccaatt gaccttctga aaaatcagaa gaagctagct cttcggtgat
    22681 ttcgtcatag taagccaata ttgactcaat ggattcgccg tatttacgtt tcaactgtcg
    22741 gattaattct aaacgttgcg taacttcttc taaacgactc tcatctaact ccaataaatc
    22801 aatctgacga ctcatatcgc cgattgcatc ttgcagcaaa tagtaagcac tttggatatt
    22861 ttcataaatt gcttcatact ccacatctag atgggcgatc ccttgcagtt ctgaaacggc
    22921 caagccaaca ccatctacac tgttttgttc attattcgtc agtgctccgt aacctgcagc
    22981 taatccatca gcaatttttt gataattgct aagtttttct ctttcttctt ttaatttctc
    23041 ttcttctccg acttgcaaat cagcagcagc tatttcttct tgctggaaat ggagcatatc
    23101 tattctctga acataggatt tttcattttg ctgcactttt cgtacccttt tttccagctc
    23161 acgataagag atatattctt gctgataact ctttttcttc tgttggaaag ctacatctcc
    23221 aaaacgatcc aataaagcaa gatgtgattc aggctggagc agttcctgat gttcgttttg
    23281 tccttggata tctactaaat aactgccgat tcttctcaga ttagctaatg tgataatatg
    23341 accattcact cgacaaacat tttttccagt taaggacata tcccgtcgaa caatcagatt
    23401 gtcctcatct gtttcgatcc ctaattcaac catcagttca gagaatcctt cttgttttgg
    23461 tagttcaaac aacccttcaa gcacacattt ttctgctcct tgacgtatat aatcgcttga
    23521 acctcttcct cctgctaaca gacctaaagc atcgataata atggatttac ccgcaccagt
    23581 ctctccggtc agcgcggtca ttccttcgtg aaaagaaagc cgcaattccg gaataatcgc
    23641 aaagttagta atactgattt cttgtagcat cacattcgct cctcaactta aaggtaatgc
    23701 aggaagtctt tcttcaataa aactgcctgg tcttcagttc gtgtaatcat caaaacatta
    23761 tcgtcatcat tgataatcga aaataattct ttcttgtaac gtttatcaat caaatttgct
    23821 gctgccgaag catttcccgg caatgttttc aaaataacaa atttttccat ttggtcaact
    23881 gccacgaaag cgtcttttaa tagtttttcc aatttagtat ttacatcttc gtttgtttca
    23941 gctggtatgc tataacggta gccgccatct gaagccggta ccttgatcag tttcagttcc
    24001 tttatatctc tggaaatcgt agcttgagta acagatattc ctttgctttt caggatttca
    24061 acaaattctt cctgctttcg gatatcttgc tcactcaaca atcgagtaat caaacgatgc
    24121 cgatcttttt ttctcattac gtcttcgctc cttagcatat tcttcttaat caatgatagc
    24181 ttatttcctt ggtataataa agaagaatcc gcatacttct cacttgattt tcacagttac
    24241 aatttattta tggtcaaact gttcgtgtgc atttgaaacc acttctgcca tattaatagc
    24301 tgaattgatt gttccgcttt cgggtacttt cctcaagtga gccaagaact cgatatttcc
    24361 ttctcccccc gtgatcggag aataacttaa ttccagcaca tcatatccac cttgtgccgc
    24421 aaatgacaaa atgtcttcta aaactttttg atgggtctga gggtcacgaa caattccgtt
    24481 tttccctaca gcttctctgc ctgcttcgaa ttggggtttg attaaagcaa tcacttctcc
    24541 ctcttctttt aaaatagcat gtaatggagg cagcatcagt tttagtgaaa taaaagaaac
    24601 atcgataaca gcaacttctg gaattcctaa ggtaaagtct tcgggttttg cataacgaaa
    24661 attcactctt tccattacga cgactctagg atcttgtctt attttccaag ctagctgatt
    24721 gtagccaacg tctaaagcat aactcatttt tgcgccattt tgcaaagctg catcagtaaa
    24781 tccgccagtg gatgcaccaa tatctaacaa tgtttttcct tcaacagata gatcaaatgc
    24841 tttgagtgct ttttctaatt ttaatccccc tcgagaaaca taaggaagtt ttttaccttt
    24901 gactcttaat tcactactca ctggaatttt ttctcccggt ttatcaaacc gctcgttttt
    24961 ttcgttgtaa atcaaaccag ccatcactga acgtttggct tgttctcttg tttcaaataa
    25021 tccttgtttg accgctaata catctacgcg ttctttttcc attctttccc tcatttctcc
    25081 acatcaaatt gcttgatcat ctggcgcatc agcccgccat caaaagaaaa aacgtcctct
    25141 tctaatttgt ctatgatctt gttggcggat aacagctcaa tctccaacgc ctcacgtgtc
    25201 ttttctaggc ctaataaacg cgggtaagta tttttttcca tacgttcatc tttaccagct
    25261 gttttcccta atgttttcgt tgtaccaatc acatcgagta gatcatctct tatctgaaaa
    25321 gctaaaccta aatgttcggc taatcgttga agtagcaata agatttcttc aggttgcttc
    25381 gctaatatgc caccagccag caaggcataa cgaatcaaat gccctgtctt tctttcatga
    25441 ataaatgcta attcttctaa agaaagcttt ttcgattcgc cctcgatatc tgcagcttgc
    25501 ccagcaacca taccttgtga tcccgcacaa acagctaatt gctgaagcag cagaagctta
    25561 ggagaattac ctaagtgtgc catactgatt agctggaatg ctcctgtaag caaaccatct
    25621 ccggccaata tagctaatgc ttcgccgaat actttatgat tggtaggttt tcctctacga
    25681 agatcatcat tgtccatcgc aggcaaatca tcgtggatca aggaatacgt atgcaccatt
    25741 tctaaagcag ctgctacttg atagacggga acatcaatag gctcatcaaa ggaagcaacg
    25801 gccgttaaaa gtaataaggg acgtattctt ttcccgcctg ctttcactga ataactcatc
    25861 gcatcataga gtcctctatc cgttgtatgc tcatctaaaa aagacaagat ttctttttct
    25921 acagcaggta gatgaatagc tgaaaagtca gttaatctca tctcagcttt cctcgtttcc
    25981 gtcaaaatca acttcttcat ttgattctgt catcattttc gtcaaggtct cttctgcttt
    26041 agctaatgta tcttggcaat gtttgcttaa ttccattcct cgtttaaacg aatctagagc
    26101 tgattctaat ggaacgtctc cttgttctaa ctgcatgaca attttttcta attcttgcag
    26161 tgactcttca aaagttggat tttccatatt tttattctcc tttgtctatc tcttgtactt
    26221 gtcctttaac gattccatct tgataatgga tcgtcagcaa atccccttga tgaatctcgg
    26281 tgacactttt tactacttga tcatttaacg tcgtatagct gtagcctctt cccatgattt
    26341 tcaatggact caacaaatca agagattgaa cagcttgagc aaaacgctgc tgctgttgtt
    26401 tcatatattg ttggattttc tcttctaagc gtttttctag ataagcagtt tcttggcgag
    26461 ctgctttaac tcgataaagc ggagccactt gttccaattg gtggcttaat gcgatcgttt
    26521 gtttttcttt cgcatgataa atttgctgtg tagcttgata taatcgctgt ttcaactggt
    26581 ctaatttgat ggtctgacct tcatacaatc tttcaggctg ccgaaataca taggaacgtc
    26641 gagcacgctc gaaccgttct tgtttgcgct ggatctggcg tacaaacgcc tgctgcaatc
    26701 tagcttgccg ttcttgaatg cgcatcaact cttctgacaa aacaggaaca gctaattctg
    26761 cagctgctgt tggagtggca gcccgaacat ctgctactaa atcagcgatc gtggtatctg
    26821 tttcatgtcc tacagatgaa atcaccggcg tattagcttg ataaatcgcc cgagcaaccc
    26881 gctcttcatt aaacggccat aaatcctcaa tcgaaccacc cccacgaccg atgatcattg
    26941 tatcgaaatt gcccatctct tctactctct ggatattcct gacaacattg tccgctgctt
    27001 ggtttccttg tacaactgtt gggaacaaaa ccaattggac aattggatat cttcttttca
    27061 ccgtcgtgat gatatcgcgg ataaccgctc cgcttggact ggtaaggacc gcgattcgct
    27121 ttgggaaacg tggcagcatt tttttaggcc cttgaaaaag gccttccttc atcagctttt
    27181 cttttcgttc ttccaattct tgatataaag cacctattcc atcaggctcc atgtgctcta
    27241 catagatttg ataagaacct cccgcttcat ataatgaaat acgcccaacg acaaggactt
    27301 tcattccttc ttttggctgg aatcgtaatt tttggaatgc tcctttgaac ataatagcag
    27361 atatttttgc atggtcatct tttaagctga aatattgatg ggcattggca cgtaaacgga
    27421 aattcgatat ttcgcctgtt aaatatacgc gttctaaata aggatcggca tcaaattttc
    27481 ttttcaaata cttcgttaat gtagtgacag ttaaatattc ttgtgccatc tattcacctg
    27541 ctccaaaagc ttcggcagat tttacggttt gatacatcag catcgtgata gtcattggcc
    27601 ctacaccctt tggcacagga gtgatccagc ctgcaatagg ctccacttca tcaaatttca
    27661 catcaccgat cagcttaccg ttagcatcac gattcatccc tacatctatc actacagcac
    27721 ctggttttac gaattctttt gtgacaaaat gtccacgtcc tattgctacg actaaaatat
    27781 ctgcttcttg tgccagttca gccagatttt cggttttaga atgagcaaca gtaacagttg
    27841 catctgccat caagagtagc tgagccatcg gtttaccgac gatattgctt cgtccgataa
    27901 ctactgctcg tttacccgcc ggatcgatag aataagcttc aaacattttc atgatgccgt
    27961 atggagtaca aggaatcttg tccggtttac ccgcaaggag tctgcctaaa ttcatcggat
    28021 gaaaaccgtc tacgtctttt tcagggcgga tagctaacag gatcttttct tcatctatat
    28081 gctttggcaa tggtaattgg accaatatac catgaaaact aggatcttga ttgtattttt
    28141 caatttctgc aagtagttcg tcttcagaaa tcgtctcagg ataacgctct accaatgaat
    28201 ggattccgat ttcttttgct acacgttctt tatttcttac atagatttgg ctggctggat
    28261 tttcccctac caataaaacg actaatccgg gatggatacc attatctttc aatttttgga
    28321 ttttttcagc aatctctgcc tgcatttttt cagctaattc ttttccgtta atcaattctg
    28381 ccatctaacc actcccagat attttttgtg ttttcattct accatagaat tttcccgatt
    28441 tataacaggt ttatgatgat tcatagaaaa aagctaggac ttacgcccca gcttctaatt
    28501 ctttcaatac attggataaa acaccgttga cgaatttacg tgaacggtca tcactaaatg
    28561 tttttgccaa ctcgattgct tcatttaaag caacagccgc tggtacatct gtcacataag
    28621 tcatttcaaa aatcgcgata cgcaagatca ccaaatccat ttttgagatc cgattgatgt
    28681 gccaattgtt ttttaaatgt ttttcaatca ctttatccaa ttcatctttt tttgcacaga
    28741 caccgccaac tagcgtatca agataaacag gaacaaactc tgattcatct tcgttgatca
    28801 tgtcgcggtg gtctaattcg attgcgttga aaatcgcatc ttcttttgtt aggtctgcat
    28861 tgaagtctaa tggaaataaa gcctgcaatg ccatttcccg aatctcgtgt cttgataatt
    28921 ctttattcat tctcttcctc ttcgtcttca aaaagttctt cgattgttgg ttgttctaat
    28981 ttttcaggaa caacagctac tacatggata ttgacttcag ataaatcgat atccgtcatg
    29041 aaaaggactt gctggctgac ccgttcctgc atttccattg ctactttagg aactgaaaca
    29101 ccatattcta gataacagta aagatctact ttcaagccat cttcctcgtt tgctaagtaa
    29161 acaccttttc cgtgagctgc acgtcctaat aattcagtga cattgctggc aaaagttccg
    29221 cgcattccgt atacgccttc tactttagaa gctgcaatcc cgataattac ttcgatgact
    29281 tcaggagcga tcacgatctc tcctaaatct tggtttgcgt ttaaaatcaa atttttttca
    29341 tctgccattt atttattcct ccctttgcct aagcacgaga tacatccgaa ttctctatga
    29401 tgcggcatag tgatcacgtg gtaagaaaac tgccgccgat cagctgcaaa ctgctatgaa
    29461 ttggagaaaa cacccaactt tattatctct tacagcttaa aagttcttct atagtgtatc
    29521 attaaatcga gtgattttct ataacaaagt ctgtgaacac tgatttttac gcttacttct
    29581 tctattgatg aaactataag atgatcaatt cttttggtga acgagtcaac acacgatttc
    29641 catcttctgt gatcaacaag tcatcctcaa tacgtactcc accgattcct ggtaagtaga
    29701 tccctggttc atcagtgatc acatttcctg gtacaaattg cttttctgca cgagaagaaa
    29761 cgtttggtcc ttcgtggatc tctaaaccga tcccatgtcc tgtagaatgt ccaaatgctt
    29821 ccccgtaacc atgttttgaa atataatctc tagcaactgc atctaattgg acaccagtca
    29881 ttcctggttt tgcagcatca ataactgcta attgagcatc taagactatt tgataaattt
    29941 cttttaattt ttccccagga tcaccaatag cgaatgtacg tgtcatatcg gaaacgtacc
    30001 cttcgtaata gcaaccaaag tccaatgtga tcaagtcacc ttgttcgatg atttttttgc
    30061 ttgctacacc atgcggcata gccgaacgca atccgctagc aacgatcgta tcaaaagaaa
    30121 cacctgaagc gccaagagaa cgcatataaa agtctagttg atttgcgact tcaatctcag
    30181 tcatgcccgg ttggatcatt ttcaggatgt gatcatatgc catatccgca atatgacatg
    30241 ctttttcgat aatagcgatt tcttcttcat ctttcacttc tctgagttct tcgatcatct
    30301 gtgaaacagg tactaattcc gcatcgatga tttcttctaa aaccgaatat tctaaaaatg
    30361 agactgttgt ttcctcaaaa gctaatgagt gcagttcttc tttgcgtact aagtttgcta
    30421 cctcatcaaa aataggtccg acatttttaa taatttcaaa cccttgagcc tgcgctgccg
    30481 cctgctcggt ataacgaaaa tcagtaataa aaaatgcttt ttccaaagta ataacagcta
    30541 atcctgtcgt acctgtaaag ttcgttaagt aacgaagatt gtacggactg gtaaccaaaa
    30601 atgaatccaa attttcttct tgcatttttt tgcgcaattt ttctacacgt agcatcataa
    30661 tgaaactccc tctttctttg cacgcttttt tccattatag caaacttttc ttcgtacacg
    30721 acagttaaat aaaaatttcc ggaaaacttc tttttatttt aaataaataa agccaaaaag
    30781 gagtcacagt ctcgctgcaa ctccttttag tttactattt tgtaaaaact tatgatcgtt
    30841 ttccaaagaa aaatgagaca actgctacca aaatcactgc gcctaaaata gatggaataa
    30901 gtgccatacc tgcagctttt ggtccccatt cgccgaaaat cgcttgtcca atacttgaac
    30961 cgactagtcc agcaatgata ttcgttacac agcccatcga atttccttta ctggtgatcg
    31021 cacctgcaat tgctccgatc acggctccaa caatcaatgt ccataaccaa ctcataatat
    31081 aacctccttc tttgctaatc aattttagca tatgggattt aaaggggaca aataattggt
    31141 tttgctattt catattttct tctgcaaagt aaaaaaataa agaaaacctc ctcttagaag
    31201 ttttctttat tttttacttg cgtttaaaca tactacgaat accacgtttc actacatcaa
    31261 agaaactcaa tgattgtacc attctatctt catctacata ttctctgatc gcacgaagca
    31321 cttttttagg ttctttggaa gaaaatgtgt atgtaccatt ttttttcgtt tgaatggcat
    31381 atcgaggaat ccattttccc tttagcatga ctgatgctag tacataatcc acttcttccc
    31441 atggaatttg gatatatttg cgtctatctc gcgcatggta aaactcaaaa ccattatcac
    31501 ctatcatgat ttgcccgtaa tctgtcagac ccgtaaacga agtagcatca attaccaagt
    31561 ctacttttgt attgattgat tgaaccattt tctccctcca gatatttgtt tatgaacagt
    31621 atactatata aaaaaagctc gggacaacgc ccgagctata tttttatgga ttataaaagt
    31681 ccgatgacgt gtccaacaac accgactacg aataaaccaa ggatgataac gattggagaa
    31741 acctttttct taagcaacca catacataac aatgttaatc ctaatgcagc taaaccagga
    31801 atcaattggt ctaagttgtc ttgtaatgtt gttacctttt caggagaaag ggctaagcca
    31861 ttattaactt gttcaaatgc actcttgatt ccttcaccac ctgaaggcaa tttatcccat
    31921 tcgatgtaag cacctttatc taattttaca gttgatacaa ctggtgagaa tttgattgat
    31981 acccatcttt gtacaagggc agctaatacg aacatcccta aaattgaagc accttttgtt
    32041 acgtcttgta acaatccgcc agaaaggtca tcagtaattt tagaaccagc tttgtagcca
    32101 aattcttgtg tataccacat gaatgcccaa cgaataatgt tccaacctaa gaagaagatg
    32161 attggtccaa ggatatttcc tcccatagct aatgaagcac ctagtgcacc tagcattgga
    32221 cgtactgtaa accagaacac tgggtcacca acaccagcta aaggacccat catacctact
    32281 ttaacccctt gaatcgctac atcatcaaca ggcgcgccat tcgcacgttc ttcttccaga
    32341 gccaatgtaa cccctaaaat tggagaagca atgtatgggt gtgtattaaa gaattccaag
    32401 tgacgtttta atgctgcaga acgttcttct tttgttttgt ataatttttt gattgctgga
    32461 atcatagaaa atgcccagcc accgttttgc atacgttcgt agttccatga accttgaatg
    32521 aaggtagagc gccacgcaac agctaaacga tctttttttg ataatttgat tttttcttct
    32581 gccattttat tttcctcctt caagattaat agtcattcaa aatgtcgcct aaagggtcac
    32641 cagagttgct tccgcctcca ttatttgatg aaccacccat tttagaaagg ttcaagtaaa
    32701 taagagctaa tgcaacacct aaagcaccta gagcaatcaa tgttaattga gaaattgcag
    32761 ctacaacgaa accaataacg aagaatggcc atacttcttt tgtagccatc atgttgatta
    32821 ccaaagcgta acctacagct acgaccattc ccccaccgat agccatacca tctgttaacc
    32881 atgctggcat tgattcaagg aatgattgca cagtttgtgc tgggataaat agcaacgctg
    32941 ctgctgggac cgcgatacga accccttgta aacatacagc aatgatgtgc cacatttcaa
    33001 ccttacggat gtttccttct tcagcagccg catccatcaa atgaacgatt ggaacagcga
    33061 ttgtacgtac gatcattgtt aagaacaacc ctgcaacagc caaaggaacg gcaatcgcga
    33121 tcgcagaagg aacaccttta acaccttgtc cacctaaaac taaaataatt gctgatgcta
    33181 ctgatgctaa tgcagcatct ggtgctacag cggcaccgat atttgcccaa cctaaagcga
    33241 tcatctgaag cgtaccgcca agtacgatac cagcttcgag gttaccggta actaaaccga
    33301 tcaatgtaca tgccactagt ggttgatgaa attgaaattc atcaagaata ccttccatac
    33361 cagctagaaa ggcaactaat acgactaaaa ttattgaaat aatagacatg ataagcctcc
    33421 tataaatttt aatattattt attacgcttt tgctaattca gcttttgctt ttttcaaaat
    33481 atcatccatg ttatcgcgtg aatcattagg tactttacgt acatcgaatt tcacaccttt
    33541 ttgttcaagc ttctcaaacg cttcaacgtc ttcttgtccc atagacaata ctttacttac
    33601 aacgacttta ccgaccgaat gcgccattga cccaacattt aattctttga tgtctacgcc
    33661 accttcaaca gctgtcaata cgtcttcagg attttcaaat aaaagcaatg ctttggtatt
    33721 tccaaaacga gggtcttttg ctacttcgat cattttgcta ataggaataa catttgcttt
    33781 tacacctgga ggagcagctt gttcgattag tctcttacgc agatcatcct ttgatactgc
    33841 atctgaaaca acgataatac ggtttggttg cactgctttt gtccaagctg tcgcaacttg
    33901 tccatgtaac agacgtgagt caacacgtgc taatacatat ttgatttttc cgtctccgac
    33961 cacagttcct tctggaagtg ctcctttagg ttgtgcgtct tcaactgctg ctttcggtgc
    34021 ttctgccggt tctaattctt ccggttttac ttttacaccg tcttttgctg tttcaatgat
    34081 gtgtgctgca atttcatgag cagattccat tgaaaagcgt gatgcgtagg cttcaatcaa
    34141 cattggtaaa ttcaaaccgc tgacgattgc ccatttatct ttgtgctctt cgaataatgt
    34201 gtttgattga ttgaacggtg ttccgcccca taaatcaact aagaataaca cttcgtcttg
    34261 attatcaaaa gaagcaaccg cttcttccaa tttggctctc aagtcatctg gaccttcact
    34321 tggtttcaaa atcacggctt tgacgttttc ttgttcgcca aagatcattg aaccggattg
    34381 taagattcct tcagcaaatt gtccatgact tgctaggata atccctacca tatttatacc
    34441 tcctatttac ttttgttgta acaaattcaa aaatattaat aattgctaac cattacaaca
    34501 gaattatttg accttttcca acaaccattc ttgctacacc tttttgattt tcgctagtag
    34561 ttccatcaaa aggattttcc tgtcagcagg tacttttcga atctctaact ccacttcttg
    34621 actgttcaag taacgaaagg aatcaatatc ttgttgattt actgaaacaa aattggtaat
    34681 cattgatttg ccttcagtaa aactcatacc gccgatattc aacgacttca actgtacacc
    34741 gcagcggaca accgcttcaa catcttgggg attggtgaat aaaagcatca cttttgctcg
    34801 tgataataat ccactatgat agatttgaat cattttctgc acggttatta catttgctgt
    34861 gatccccggc ggtgcagctt cttttagtag aaactttctc aatttatctt gagcgactac
    34921 atcgctgatc acaatgatac gttcaacacc tgtttgcttc gaccaaactg ttgcgacttg
    34981 cccgtggatt aaacgatcat ctatacgtgc caatcgaata tccatctgca aaactccttc
    35041 ttcctcacta cttcacagaa acccctatca tttctgtgtg aacaaccacc tcctagcaca
    35101 cactactata agcaaaaagc gtgccaactt ttttacgata cactaattga atttaaagga
    35161 ttcttaacca atacacaaaa acgatacact ataaaagtgt atcgtttttg tgtattcatg
    35221 gcacagtgta ttctttttta tctaactgat tatggactag acgtagcaga taatacattt
    35281 cttctatcgg cacttttact tttatctgat cttccaacag aacaactgag gctgccaatt
    35341 ggctataaag aggatcattc gttgtatttg tcaattcgtt ttcttccaca gtcaatggtt
    35401 cattccgaat aatacgttcg atcattcctg ctgtgtgcat cactaggttg attttgaatc
    35461 catattcttc atcgccgatt cccgtcgtct gagagatttg tgtagaaaat tgccacattg
    35521 gatcgattaa cttactacca ttaataaaga tgaagttatc agagataaac tcaacacacg
    35581 tatttttagc tgactcttca tctagggaga tgaattcagt ttcctccaat tctgattcca
    35641 tcagcaactg atccaaaatg acttcgatat tttgatcaat aaaacgttct aaaggaataa
    35701 atggggcttg gatattcaaa tcagtaatcc cagtagtagc tatgatctga tattcttttt
    35761 ggataactgg cagttcttct ttcacttcca caatcgacaa agctagaact tctaattctg
    35821 tttcttgacg cttgtttacc gctcgttcga tcaattcttt gattctttgt gctgtacctt
    35881 cgccagatgc gcagatagcc aaaacagctt ttttcttatg aataggtttt gcttcctgaa
    35941 cagattctac agaagatatt tctgcgtaac ctcgaaaatt tttcaatgat tcatgcaaca
    36001 tatctagatc tgtccctaat actgagacct ttcgtacagt ttccaaaact agagaagttg
    36061 ttaccatatc aacagtacgt actggaattc cagtgtctct ttgaatttgc gaattaaacg
    36121 acgcaaggga ccccatatct actaagagta tgaccccgct tccttcatct acttcttgta
    36181 cattgcgttc aattcggtcc aatgctgttt tcggatccat atccaaaggc atgtctactg
    36241 ctcgtacgtt ctctgcatct aacaactgtt ggacaacttg gaccatactg cttgctgtgc
    36301 tgtttccgtg agctgcaacg actacaccga ttctgccgct tgcttgattt gcccgtaacg
    36361 agacgagtag aacggtcaag taatagtctt cactttctgg aatctccact tgaaaatacg
    36421 tttcgatata actacgcact tctttcgcaa cagcatattc atctggaaaa tcaaaagcca
    36481 tctcacggat gttatcattc gtatgccgtt cttctcctaa gttgattttt ttcaaaaaag
    36541 aactgatatg caagctcata gcataaataa agttttgctg aaactcatat ggtaaagatc
    36601 ttttgaccat tacataaatc tgattggtca cttctatgat tttcggatct acaaattctg
    36661 ctaatttgct gtctgcatta aaagaaaaac catggtcttt atagaaagac ttcagatgga
    36721 tgttgatatc tgtagaaatg aaatgattga ttgcttcttg atccaaacca tccgacttca
    36781 gtaaagctgc tttatctccg atgatatcat atagattata aggtaactca taggagtccg
    36841 attggatttt catgatcgta tcatttggtg ctacagttat ctttggctcc aatattttag
    36901 aaagctctgc cattgcactg cggttgcttg ctaattgaat caatccgcta cgaatccctt
    36961 ctgtcaaatc atcgattgtg atcgagattt cgggtgattg catatgattt agaaatcctc
    37021 gtgcacatac taactggata tttgacttca attgtcctat atttccatag gtcacactgc
    37081 cgatcaatgc ttttgctaca tcttctgtca aagaaatctt tcgctgcgtg cggtttgctt
    37141 cgtgggcaat catgattttt actaaatcga ctttctcatt tgcaggacgt ttttcaaaag
    37201 ccggaagttt gatattgatt gggatacgcc gaacaaaagt ttctaataga gaagaacctg
    37261 ggtcttctgt ggtcgcacct acgatccgta catttgcttc atgggatttc gttgtttccc
    37321 caagacgact gtaagttcca tgatccataa agtaaaagat catttcttga ccttcaggcg
    37381 gcaaccggtg aatctcatcc aaaaacagca taccgccatc cgcttgatcg ataatcccca
    37441 ttttttcttc atctgcgcct gtaaaagcac cttttgcgta cccaaataaa tgactcatca
    37501 aaagctcagg gttattggca taatcagcac aattgaaaac aatcagttcc gtttcctcat
    37561 ctattacatg atgcgctttt gcaaaatgat acatcgcgtg tgcaaaataa gtctttcctg
    37621 atccggtagg tcctgtaatc aaacaattta atcctcttgg cggatacaaa atcgcagctt
    37681 ttgcctgttc cactgaattt ttcatgctgc cgttagctcc aatgattttt gcaaagatat
    37741 ccttagtatc tatttggagt gtattttttt cttcagagac cggcgtttct ttgtaactga
    37801 ctacatgttt tgtaactggc tttacgtgcg taacgagtgt ttgatatatg taacgtactg
    37861 gtcgtccgtc tgtctttatc agttttcctt cgcgcactag ctgattcaga tctttactgc
    37921 tatttgttcg ctgaatccct aacacttcag caatttcctg tgttgttact ccttgatcat
    37981 attctgcctt tgttaaatga gcggtttttt ctttgacata catataaatt cggtcaatgc
    38041 gttttgccaa tggtatcgct ccttttctat agactattgt acttaggctt caaaacataa
    38101 tactatgtat ttatttctgt ttcgtcaact atttacctga ttttgttttg ttgataacac
    38161 gcggatagat agaaatgttc aatttaaaag aacgaaaaat cccgatacga tttttataat
    38221 ggaaacaaga aaaagaacac tgtttaccat ataattattt cactgatagc aaacaatata
    38281 gcttccatca attgaaaacg atagttatca aaacatcctg attccaaatt ggcaaaaaaa
    38341 tattaagtgt aaaaagacaa agtaaacttc gtagcctcca ctacaacttt tatttttata
    38401 aacaaaaaag gctgtgacaa aagtcttgtc acaacctctt taatcataag attcaattaa
    38461 gcttcttgag ctactggata aacagacact tgttttttgt tacggccttt acgttcgaaa
    38521 cgtactacgc catcaacttt tgcaaataaa gtgtcgtctc caccgatacc cacgtttaca
    38581 cctggataga tttttgtacc gcgttgacgg taaagaattg atccacctgt aactgtttgt
    38641 ccatcagcac gtttagcacc tagacgtttt gattctgagt cacggccgtt agatgtagaa
    38701 ccgccacctt ttttgtgggc aaataattgt agattcatag ataacaacat ggtctgcacc
    38761 tcctatttat cattaattgt tttaatttgg atatattctg agttttcagc ttctatcgat
    38821 tgtaatccta ataaaagatt ttctaataga atctcagcaa tattgttttg ttcttgtgtc
    38881 attcctgaag tgacttcggt ataaagataa ccgccttctt cttcattcat ttcaacaatc
    38941 ggctcaaaac cggctaatga ggcaatcccg ttgacagtgc tgatcgctaa cgcagaaaca
    39001 cccgcacaaa cgatatcact tccataagga ccagcatcag catgcccggt caaagtgaat
    39061 gaaacgatct gacctgcgtc atttcgctta aatgttcctt tgatcatata taggccttct
    39121 ttctcaaacg ctattaagcg ttgatcgcgt cgataaccac ttttgtatat ggttgacgat
    39181 gaccttgttt acggtgtgaa tgttttttag gtttgtattt gaaagtaacg actttctttt
    39241 gtttgccgtg tttttcaaca gtaccttcaa cagttgcacc tgcaacagtt ggagcgccta
    39301 cttttgtaga ttctccgcct actaagataa cttcgtcaaa aacaactttt tctcctgctt
    39361 caacgtcaag tttttcaaca tagattgctt gtcccacttc aacttttact tgtttaccac
    39421 cagttttaat aattgcgtac atgctataaa gcacctcctt ctgatatact tagactcgcc
    39481 atccgaagtg acagcatatg ctgtgcttat tacttgttct gagcggttgt agctgtggag
    39541 tccacaatta caacagtact agcttaccag aacagcaacc gttagtcaac taaatttaaa
    39601 atcaaaaagt aaaacaaaaa ttcaagcaat acttctgctt ttctatcctc acagttctac
    39661 caaataaaat agtcgcattg tgtttctttt attcttataa taaaagaaaa aggaggagca
    39721 taaatggaat acccaaaaga actgatacaa gaagcttctt taagaatgaa cggccgtcct
    39781 gtagaaggat ttatcgctgg gcaaggacct cttcatccta aactgatgct cgtgggagaa
    39841 gccccaggga aaacagagat tgatacgcat gttcctttta gcggtcaagc tggaaaagaa
    39901 ttgatgcagg cactttcttc aactggctta acacgagaag aggtgtatat caccagtgct
    39961 gtaaggagcc gcccgtatcg cgtgactcat cggatcaata aacggacaca acagtcagaa
    40021 actgtttatc caaatcggac acctactcgt tccgaagtat ttgcgcatgc accaatattg
    40081 gattacgaac tgatgaaggt ccagccaaaa ttaattgcga cacttggaaa cattggtctt
    40141 caacgtttac ttggcaaaga atatacggtt accaaaaatc atggtcaact ctatacaggg
    40201 cctgtcttac aattaacaga acaaaaggat gcctacgaat ggtcagaaaa aaactatcga
    40261 attcttccac tttttcatcc agcagctatt ttctacaatc gctcccttgt acctgttatc
    40321 catcaagact ggcaagtact agcttcactt ttacagcaat attaaaaaga acagacaaga
    40381 ccataaaatc ttcagtttac agtcttgtct gttctttttc attgttttaa tggctaattg
    40441 cagtatattt cgcatgtaaa gctgtcaacg catgagcaac tgtttgatgt tgatgcgtga
    40501 atgtatgttc ccacaagtga gaaagttgtt tttctacttc tggctgcaag gaagtataga
    40561 taatcgtttg tcctctttgc tctgcttttt cttgaaattc atcaaaaact gcacgtgccg
    40621 tcaaatcaat acttggtacg cctcgcatgg agaaaatgac accatcgtga tgatttagtt
    40681 cttccaattt atttttgagt ttttctgaag acataaaaaa cagtggacca ctaatataca
    40741 ctactgtcca gttattcagc ttttccgttt ctggcagccc cattctttgc caatcgatcg
    40801 tttctaatga aatcgtgata gcagcgcttt tgacaacaaa aaatacacag ccgccgatca
    40861 ccccgatcac gatagcaata cttaaatcaa agataactgt acaaatcatc gtaaggataa
    40921 acaatagaac tgctgaccaa tatttattag taaatagctc cttgatcgtt tcccattcat
    40981 tcatacgcca agccgtcaca atcaaaacac ctgcaagtgc tggcattgga atctgtgaca
    41041 tcactggcgc aaaaacaagc atggagagca gtaagacaac tgcatgtatc ataccagcta
    41101 atctagtttg tgcaccagat ttgatggcca cgcttgtacg ggcgatggcc gcagtagcag
    41161 gaatcccgcc aaagaaagga agcaacagat ttccaattcc ttgcgccatt aattcttgat
    41221 tgctatctaa gggcttattt gccattcggc cagccgaagc accacatagc aagctttcaa
    41281 tcattcctaa taaagcaatg ctaatagctg gaactgccac ttgttgcaag gcagaaaagc
    41341 taaaatctcc catattcaaa cgattactac tgatcaatgt ttgtggaatc ttacccacag
    41401 ttgctactgg taaatggaac aatagcataa ctgctgttgc caaaatgata gccagcagcg
    41461 aactaggcat tcgtttcgcc catttttttg gatagataaa catccctaaa atgactagcc
    41521 ctcccatgat caaagaggct gtgtttatcg gaaatcctat cgtttgataa ctacttagtt
    41581 tttcaataac gtttgtacct tccgagtgaa caccaaataa attatcgatc tgacctaatg
    41641 caataatgat agcaatacct gaagtaaacc ccgtaattac gggtgctggg ataaaagaag
    41701 tcagtgttcc taatcggaaa agtcctgctg caagtaaaaa tagacccgct aaaaaagtag
    41761 caagcaatac accttgcatt ccctttgttg ccgcaataga catcaagata gctgccatcg
    41821 cacctgtcgg acctgatatt tgataaaaac cgcctgataa accgccaata acgataccgg
    41881 caatgatagc cgtaatcaaa cctgcggccg catcagctcc gcttgatacc ccaaaagcca
    41941 aggctaaagg caatgctaca gcagctactg tgatcccagc caaaagatct ttctgaaatc
    42001 ttgaaagatc ataacctgaa aattcttttt ttagtgagga aacataattt ttgaacacct
    42061 atctaactcc tttttcatga aattttctaa aataccattc attaga
//
LOCUS       DADXTG010000011        40080 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.011, whole genome shotgun sequence.
ACCESSION   DADXTG010000011 DADXTG010000000
VERSION     DADXTG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.011"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            104..274
                     /locus_tag="IWI57_000467"
     CDS             104..274
                     /locus_tag="IWI57_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448635.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            494..1201
                     /locus_tag="IWI57_000468"
     CDS             494..1201
                     /locus_tag="IWI57_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2448636.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1260..1904)
                     /locus_tag="IWI57_000469"
     CDS             complement(1260..1904)
                     /locus_tag="IWI57_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBC2448637.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHGQLITDHI"
     gene            2031..2318
                     /locus_tag="IWI57_000470"
     CDS             2031..2318
                     /locus_tag="IWI57_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448638.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2426..2746
                     /locus_tag="IWI57_000471"
     CDS             2426..2746
                     /locus_tag="IWI57_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2448639.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2827..4140
                     /locus_tag="IWI57_000472"
     CDS             2827..4140
                     /locus_tag="IWI57_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBC2448640.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            4137..4856
                     /locus_tag="IWI57_000473"
     CDS             4137..4856
                     /locus_tag="IWI57_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBC2448641.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4984..5535
                     /gene="raiA"
                     /locus_tag="IWI57_000474"
     CDS             4984..5535
                     /gene="raiA"
                     /locus_tag="IWI57_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBC2448642.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5757..8291
                     /gene="secA"
                     /locus_tag="IWI57_000475"
     CDS             5757..8291
                     /gene="secA"
                     /locus_tag="IWI57_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBC2448643.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8417..9518
                     /gene="prfB"
                     /locus_tag="IWI57_000476"
     CDS             join(8417..8488,8490..9518)
                     /gene="prfB"
                     /locus_tag="IWI57_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBC2448644.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9623..10309
                     /gene="ftsE"
                     /locus_tag="IWI57_000477"
     CDS             9623..10309
                     /gene="ftsE"
                     /locus_tag="IWI57_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBC2448645.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10293..11186
                     /locus_tag="IWI57_000478"
     CDS             10293..11186
                     /locus_tag="IWI57_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448646.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11338..12786
                     /gene="cls"
                     /locus_tag="IWI57_000479"
     CDS             11338..12786
                     /gene="cls"
                     /locus_tag="IWI57_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBC2448647.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13123..13980
                     /locus_tag="IWI57_000480"
     CDS             13123..13980
                     /locus_tag="IWI57_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBC2448648.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14095..15015
                     /gene="pstC"
                     /locus_tag="IWI57_000481"
     CDS             14095..15015
                     /gene="pstC"
                     /locus_tag="IWI57_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBC2448649.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15015..15899
                     /gene="pstA"
                     /locus_tag="IWI57_000482"
     CDS             15015..15899
                     /gene="pstA"
                     /locus_tag="IWI57_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBC2448650.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15914..16720
                     /gene="pstB"
                     /locus_tag="IWI57_000483"
     CDS             15914..16720
                     /gene="pstB"
                     /locus_tag="IWI57_000483"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBC2448651.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16736..17494
                     /locus_tag="IWI57_000484"
     CDS             16736..17494
                     /locus_tag="IWI57_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBC2448652.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17507..18184
                     /gene="phoU"
                     /locus_tag="IWI57_000485"
     CDS             17507..18184
                     /gene="phoU"
                     /locus_tag="IWI57_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBC2448653.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18435..19453
                     /locus_tag="IWI57_000486"
                     /pseudo
     CDS             18435..19453
                     /locus_tag="IWI57_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            19450..20217
                     /locus_tag="IWI57_000487"
     CDS             19450..20217
                     /locus_tag="IWI57_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBC2448654.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20317..21885
                     /gene="liaX"
                     /locus_tag="IWI57_000488"
     CDS             20317..21885
                     /gene="liaX"
                     /locus_tag="IWI57_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBC2448655.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            22182..22502
                     /locus_tag="IWI57_000489"
     CDS             22182..22502
                     /locus_tag="IWI57_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBC2448656.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22502..22852
                     /locus_tag="IWI57_000490"
     CDS             22502..22852
                     /locus_tag="IWI57_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBC2448657.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23019..23957
                     /locus_tag="IWI57_000491"
     CDS             23019..23957
                     /locus_tag="IWI57_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBC2448658.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23972..24802
                     /locus_tag="IWI57_000492"
     CDS             23972..24802
                     /locus_tag="IWI57_000492"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBC2448659.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24846..25871
                     /locus_tag="IWI57_000493"
     CDS             24846..25871
                     /locus_tag="IWI57_000493"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBC2448660.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25886..26824
                     /gene="galU"
                     /locus_tag="IWI57_000494"
     CDS             25886..26824
                     /gene="galU"
                     /locus_tag="IWI57_000494"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBC2448661.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26949..27389
                     /locus_tag="IWI57_000495"
     CDS             26949..27389
                     /locus_tag="IWI57_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBC2448662.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27392..27994
                     /locus_tag="IWI57_000496"
     CDS             27392..27994
                     /locus_tag="IWI57_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBC2448663.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(28053..29159)
                     /locus_tag="IWI57_000497"
     CDS             complement(28053..29159)
                     /locus_tag="IWI57_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBC2448664.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            29369..30370
                     /gene="ccpA"
                     /locus_tag="IWI57_000498"
     CDS             29369..30370
                     /gene="ccpA"
                     /locus_tag="IWI57_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBC2448665.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(30707..33052)
                     /locus_tag="IWI57_000499"
     CDS             complement(30707..33052)
                     /locus_tag="IWI57_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBC2448666.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            33331..34233
                     /locus_tag="IWI57_000500"
     CDS             33331..34233
                     /locus_tag="IWI57_000500"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBC2448667.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            34446..36221
                     /locus_tag="IWI57_000501"
     CDS             34446..36221
                     /locus_tag="IWI57_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBC2448668.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(36287..37873)
                     /locus_tag="IWI57_000502"
     CDS             complement(36287..37873)
                     /locus_tag="IWI57_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBC2448669.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            38124..38348
                     /locus_tag="IWI57_000503"
     CDS             38124..38348
                     /locus_tag="IWI57_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBC2448670.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38490..>40080
                     /locus_tag="IWI57_000504"
     CDS             38490..>40080
                     /locus_tag="IWI57_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2448671.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
BASE COUNT        12991 a         7074 c         8485 g        11530 t
ORIGIN      
        1 cgttagttcg agagctcttt tttttgcttt gctaccttgt tccttgaaga ttctgctgaa
       61 ttaagataga gttaagttaa ctacaagaat tggagggatc cacatgaaat ggaaagaaaa
      121 agtagacgaa gcagctgaaa agttatatga tttggtcaaa tcagaaaaat acaatattga
      181 agtgaatatt ccgaaaaaag gcggaaaagc ggtccaggta aaatcaaaac gcccaacgaa
      241 tcatacaaaa aaatggatgg ctaaaaaccg ctaataataa caaaagatcc ataagtcggc
      301 attttcatcg attttagggt cttttgttat tttctaggtt gaacatatct aaaaaattgt
      361 ttctgacaat aggagtaggg agaacgatgt gtgtctaaat cctctgttga tggatagaaa
      421 cctttatctt tttcagttgt gaatcaggta tataccagtt ataatagaag aaacgaaagg
      481 acgggtgtac gttatgtcga atcaattacc tgtatatata cagattcatg accaattaaa
      541 aaatgaaatc gaacagggta tatggaaagt cggagaccgt cttccttcag aaagagaatt
      601 agcagtgaaa ttcaatgtca gccgtatgac gttgagacaa gcaattcaaa acttggcaga
      661 tgaaggaatc ttagaaagaa agatcggttc gggtacgtat gttgcgcgag aaaaagtaca
      721 agaaaaaatg tctggtgcaa caagttttac agaaattatg gaatcacaaa atcgagtacc
      781 gtctagtcgg acaatctctt atttcttgac ttcgccaagc tcaagtgaaa tggaaaaact
      841 aaacttgagt aaagatgaca cgatcttgcg aatggaacgt attcgtttcg cagatgatgt
      901 ccctatttgt tttgaagttg ccagtgttcc acaaaagctg atcgatggct acagcaaagc
      961 agagattact gaatcgttgt atcgcacgct agaagaaaaa ggcgggaaaa agatcggtcc
     1021 agcgaatcaa acgattactg ctgtgttggc ttctgagaaa attgctgact atttagaggt
     1081 aaaacgagga gatgcagttc ttcgtttgcg ccagatttct tattttgaag acggtacgcc
     1141 gtttgagtat gtgcgtacgc aatacgtagg aaatcgtttt gagttttatt tggaaaaata
     1201 aaaaacaggt gatccctgat tccttattag aaataaaaga gaatcagaat cacctgtttt
     1261 tatatatgat cagttatcag ttgcccgtga taagaaacag aaccctcttc aaattggact
     1321 tgtccgttca gcagctcaat gatggattct ttaaagtggt cagcactttt ttcatctaca
     1381 gcacagatga cgctaacttg atcagtatat tggatttctt ttataaaaaa atgttggagt
     1441 tcaagataat tctgtaattt tcctagttgc ggatacgtga tcatcacaga gatctcctgt
     1501 tgcaaggtac cttctacgat tccaatgtca gacaatgctt gagagaccgc atgagtgtat
     1561 gctcgtatta atccgcctgt gccgagtttc gttcctccaa aatatcgagt gactactgca
     1621 gctacattga tcagttgatt ttttttgagg acttccaaca tcggtacgcc cgccgttcca
     1681 cttggttctc catcatcgct gcttcgctgg atttcatttt tttctccaat gatgaaggcg
     1741 ctgcagttat ggttcgcttt ccaatgttct ttttttattt cttgaatgaa actttttgct
     1801 tggtcctctg attctattcg tctaagagaa cagataaagc gtgatttttt gatctcaatc
     1861 tcactttgtc cattttcttt tattgtacga tagcgctcga tcatcttgtc acctcttttt
     1921 ttcattttct ttattcagta taaataaagt tctaggttgt ttcaacactg taacggttgc
     1981 gcaattgctg tagtatgcga taataaagac aaaggcaggt ggatctgggg atgcaggaac
     2041 gaatgaaaaa gtacgatgca attacgcatt atttgaagaa caatggcggc tctcaagtaa
     2101 cgttgacctt tacccaattt gacgaactgc tgtttcccag taacggactt cccaaaacag
     2161 caagggaaag tacagattgg tgggcaaatg attataaaca ccctgaaaaa ggtgcctatg
     2221 gctggataaa tgcaggctat gaagtagttg tcatcaattt ggacaaagaa tatgtcgtgt
     2281 tcaataaatt agtgaaatct agttggctat ttgattaaat aaaccgcgtt tagaaaacgt
     2341 tgacaaattt ctttcttttt atataataga gtaacagacg cgaagaaaac gtttgcatag
     2401 tttgttttaa aaaggagaga aaagtatggc taaaaaaaca atcatgcttg tatgttcggc
     2461 tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa gctgctgaag caaaaggaat
     2521 cgactcggat attttcgctg tgtccgcttc tgacgcagat aacaatcttg caaataaaga
     2581 cgtggatgtg ttgttattag gcccacaagt tcgctttatg aaagctgatt ttgagaaacg
     2641 tctggaacca aaaggcattc ctttagatgt catcaatatg gctgattatg gtatgatgaa
     2701 tggcgaaaaa gtgttagatc aagcaatcac gctcataggt gaataaatag aacagtataa
     2761 ctatcgaaca agcgatccaa aaaatagatc gcttgttttt tcgtatattt ttttagggcg
     2821 aagaatatga atcatttttg gggtagaagg cttctgacga gagatattga agcaatagac
     2881 tgtgaggagg taaatttacc aaattatgag aagatcgcag cagtggaacg tttgggcaac
     2941 tggattcttt gtcatcgctg tggaatgagg acgtccgtca ttgatggaca gcttgcagat
     3001 tacggttatt tttgtacaca ttgtctatat ttagggcgat gcgacactca acaagattta
     3061 tatttgtttg atcagcctga agcaaaacct cgagaagtcg tctttttttg gacaggacag
     3121 cttactgaaa aacaaacgga aatctcaaag agaatacttt gccattcaga aaaaagacat
     3181 catcttattt gggctgtaac aggtgcaggg aaaacagaga tgctttatcc tattttagtg
     3241 gaaactttaa aagctggagg aagagtagcg atttgtacac cgcgagtcga tgtttgtaat
     3301 gaattgtttt tacgctatcg ttatgttttt ccagaagaaa aaatcacgtt gctccatggg
     3361 aatacagcaa cggcttatac ctttacttct tttgttatct gtacgattca tcagctatac
     3421 cgtttttatc ggtcctttga tttaatagtg atcgatgaga tcgatgcgtt tccttataac
     3481 ggggatgcag gattagctca tgctgtgcaa acagcgatta aaccagacgg acgattcatc
     3541 tacttaagtg ccacacctga taaaaagtta ctaaaggaaa tcaagcagac gtttgacatc
     3601 cacaaacttg ctttacgctt tcatcggcgt ttgcttcctg aacccgtctt ggttttcatg
     3661 aataattggc gacaaaaatg caagaacaag aagaagataa aatctttggt gcggatcatt
     3721 catcaattaa ttaaagaaaa ccatatcctt ttattttgtc caagtatcac gatgatgcat
     3781 cgtttgaaag ccacacttca agaggctctt ccgagatatg agatcacaga tgtttcttca
     3841 agagacaatc agcgggcgga aaaagtccag aaaatgcgag aagcaaaata tgacatactg
     3901 ttaaccacga tgatccttga acgtggagtc acatttgaaa gaatatccgt tatcgtttta
     3961 ggagcggatc atccggtttt tactaaatct tctttggtac aaatcgcagg aagagccgat
     4021 cgtaaagggc cttatacgaa cagtcaagta tactttttct atgaagaaaa aactgcagct
     4081 attcgaaaag tttgtcagga aatcaaagaa atgaatgaag aagggaagaa gatgctgtga
     4141 ggtgcagttg ctgcagtaaa gatatcttcc aaaatttaac attcagcgag attattggtg
     4201 tttcgaaata cagattacct cagacttgtt cagtttgtcg cgcacaattt catttattgg
     4261 aaacaagagg ctgttctggc tgtcaaaaac caatccattt caatctagag agtacgaaaa
     4321 agaaagtaaa gtctattccc gtttactgca gtgactgtca agaatggaag aagaaatatc
     4381 cgacctatga ttttcagcac cgtgcctttt ttgtgtacga tgaaaatatg aaggcctggc
     4441 tcaatcaata caagtttatg ggagatatcc gactagcagg cacctttgca tatagatgga
     4501 cgcagataaa aaaagaatac ccgacataca tctattgtcc aataccttta tctgaaaaaa
     4561 ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt gaaagcagca agaattcctt
     4621 ttcagatgct actcaaaaga gagaagcatc tgctggctca agcaaaaaac acgcgaaagg
     4681 aaagattgga gatgcctcaa ccttttgaac ttgcagtcac cttttctaag atacaaaata
     4741 aaaaaatatt gattattgat gatgtctata caacaggaca gactcttttc cacgcagcag
     4801 attgtttgca ggtcgcacag ccgaaaatca tccgaaccat ttctttggcg agatgaatta
     4861 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
     4921 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
     4981 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
     5041 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
     5101 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
     5161 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
     5221 ttggttgttg ataaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
     5281 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
     5341 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
     5401 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
     5461 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
     5521 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
     5581 agctgccgca agtttttttg agtttcctcc tcttttagat gaaatatgaa taaattatga
     5641 agttcttact ttaatacgct taagatattt caattatcta taactaatga taaaatgaaa
     5701 agtaaagtct acatagtctg gataatacac agaaggaaaa ggagattaca caacgcatgg
     5761 ccaatttgat tcgttcactt atcgaaaacg ataagaaaga actaaaacgt ttagatagca
     5821 ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc attaactgat gaacaattac
     5881 aggcaaaaac tgctgaattc aagggcagat accaaaaagg agaaacatta gatcaattac
     5941 ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg tgtattaggt ttatatccat
     6001 accacgtaca attgatgggt ggaatcgtgc ttcacgatgg aaatatccct gaaatgcgta
     6061 ctggtgaagg taaaaccttg accgcaacga tgccagtata tctaaatgct ctgactggcg
     6121 aaggggtaca cgtagttacc gtcaatgaat atttagcaac ccgtgactcg acggaaatgg
     6181 gcgaacttta taatttctta ggattaacag tggggttgaa tatcaactca aaatcttcgg
     6241 atgaaaaacg cgaggcttat aattgtgata tcacttatag tacgaataat gaattaggat
     6301 ttgactactt gagagacaat atggtcgttt accgccatca aatggtacaa cgtccgctga
     6361 attatgccat tgtcgatgaa gttgactcaa tcttgattga tgaagcgaga acgccgctga
     6421 tcatttcagg gcaagcagaa aaatccacag ctttatatac aagagcagac aacttcgtga
     6481 aacgtttgaa agaagaagaa gattataaaa tcgatgtaca atcaaaaaca atcagtttaa
     6541 ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt ggataactta tacgacattg
     6601 aaaatactgc attgacccat cacttggatc aagcattgag agccaactat atcatgcttt
     6661 tagatatcga ttatgttgtc caagacggaa aagtactgat tgttgaccag ttcactggtc
     6721 ggatcatgga tggacgccgt tattcagatg gacttcacca agcaatcgaa gctaaagaag
     6781 gcgtagagat cgaagacgaa acgaaaacca tggcgacgat cactttccag aactatttcc
     6841 gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa aacagaagaa gaagaattca
     6901 gagaaattta taacatccaa gtataccaaa ttccgacaaa tcgtccggtt atccgtgatg
     6961 atcgtccaga tttgctttac ccaacattac aaagcaagtt ccacgctgtc gtgcaagata
     7021 tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg aactgttgcg gttgaaacat
     7081 ctgagttatt atcagatatg ctgaatcaag aacgtattcc tcatgaagtg ctgaatgcga
     7141 aaaatcactt taaagaagca gagatcatca tgaatgctgg tcaaaaaggt gctgtgacga
     7201 ttgctaccaa catggctggt cggggaacag atatcaagtt aggacttggg gtaagagaac
     7261 ttggtggtct agcagttatc gggacagaac gacatgaatc tcgtcgtatc gataaccagc
     7321 ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc acagttctac ttgtcattgg
     7381 aagatgattt gatgaaacga ttcggttcag aacgaatcaa agctttctta gatcggatga
     7441 agatcggtga ggaagatgct gttatccaaa gcaagatgct gacgaaacaa gtagaatctg
     7501 cacaaaaacg tgtagaagga aataactatg acacacgtaa aaacgtctta caatacgatg
     7561 atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg acaagaagta atcatggaag
     7621 aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg gacaatcagt cgtgtagtag
     7681 atgcgcatac acaattagag aaagaaaatt ggaatttgga aagtatcatc gattttgccg
     7741 gcagtactct tgtccatgaa gatagcatta ctcttgcaga tatcgaagga aaaacagctc
     7801 aggagatcaa agagtatcta aacgaacgag cacaagaagt atttaatacc aaagcggctc
     7861 aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt cgtgatcttg cgtgttgtgg
     7921 actctaaatg gacagaccat attgatgcaa tggatcaatt acgtcagtca atcggcttac
     7981 gtgcttatgg acagaacaat ccattagtcg aatatcaaac agaaggctat aagatgtttg
     8041 aagatatggt aggcgccatc gagtatgaag tcactcgaat ctttatgaaa gctgagatcc
     8101 gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc ttctcactca gaagacggag
     8161 atgccaaaga aaatagtaat actagcgcga aaaaacagcc aatccatgtg aataaggtcg
     8221 gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata taagaactgt catggaaaaa
     8281 atgcaaacta atttctgaca gcaggattgg tgagacaaat tcttttttta agaattcgtc
     8341 tcaccttctt tctgttattt gctgattctg ttagactaga tataatgaaa aaagtatagt
     8401 gaaagaagga aaaaacatgg aaatatccga aattcgaaat ctcctgacag aaatgcagga
     8461 aaaggtcaca agtttcagga ggtctctttg acttagatca gctggaagaa gacatcgcgg
     8521 aagcagaagc aaaaatggct gaacctggtt tttgggatga cagcgaagcc gcacaaaaag
     8581 tgatcagcga aaataacgct aataaagaaa cctatgacca attccattct cttgccgaag
     8641 aactagaaga aatcgaattg atggtggaga tgctgcaaga agaacctgat gcagcaatgc
     8701 aagaagaagc agaaacacgt atcaatgcat tagatgaaaa aatgaagacg tatgaattat
     8761 ctatgctgtt agacggacct tatgatcgaa acaatgcgat cgtcgaattg catccagggg
     8821 ctggcggaac ggagtcccaa gactggggca gcatgctttt acgtatgtat acacgttggg
     8881 cagagcagca tggctatcaa gtagaaacac tagattatca agcaggtgat gaagcaggga
     8941 tcaagagtgt gaccctgctg attaaaggac ataacgcttt tggttatcta aaatcagaaa
     9001 aaggtgttca tcgccttgta cggatttcgc cttttgactc agcgaaacga cgtcatactt
     9061 ctttttgttc ggtagatgtg atgccagagc ttgatgaaac gatcgatata gaaatcaatc
     9121 cagatgattt gaaaatcgat acgtaccgag caagtggtgc aggtggtcag cacatcaata
     9181 aaacagaatc tgctgtacgg atcactcact tgcctacagg aacagtagta gctagtcagg
     9241 cacaacgctc acaattgaaa aaccgtgagc aagcgatggg gatgttaaaa gctaagcttt
     9301 atcaattaga agtggaaaag aaagaacaag aagcagctgc acttcgagga gaacaaaaag
     9361 aaatcggctg gggttcacag atccgttctt atgttttcca tccctattct atggtaaaag
     9421 accatcggac aaacgtagag acaggaaatg tccaagcagt tatggacggt gaccttgatt
     9481 tatttattga cggctattta aaaatgcatc tacaataaga acccattgtt aaattgaaat
     9541 aaaattgtaa gattttgcaa gcatgttgaa acattgccat gctataatag ctaaagactg
     9601 aaaaagatat ggagagaacg ccatgattga aatgaaggat gtaatgaaga agtactcaaa
     9661 tggtacgacc gctatccgca atctctcgat tgtcatcgaa caaggagagt ttgtctatgt
     9721 ggttggtccg tctggagccg gtaaatctac attcatcaaa ttgatgtatc gagaagaaaa
     9781 agcttcaaaa ggaactttga acgtcgcagg tcatgatcta atagcaatca aaaaccgcga
     9841 agttccttat ctacgcagag aaatcggcgt ggtgttccaa gactataaat tattaccaag
     9901 aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt atcggcagaa aacctcgaga
     9961 tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt ttgaaacaca aagttcgagt
    10021 ctttcctagc gaattatccg gtggggaaca gcaacgtgtc tctattgctc gggcaatcgt
    10081 gaatacgcct aaagtactta tcgcggatga accgacagga aatctggatc cagaaaactc
    10141 atgggaaatc atgaaattat tagatcggat caatgcccaa ggaactactg tcgtgatggc
    10201 tacacataac agtacgatcg taaatacgat tcggcaccga gtaattgcta tcgaaaacgg
    10261 ccgcattatt cgagatcaag cggaagggga atatggatac gatgattaga acctttttcc
    10321 gccatttatt agaaagtatc aagagtttga aacgtaacgg ctggatgaca gttgcttcag
    10381 tcagtgcggt aacgatcacg ttgactttag tcggtgtatt catggcagtt atcatgaatg
    10441 caactaaatt agctcaggat atagaaggaa acgttgatgt atctgtattc gtagatatcg
    10501 gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt gaatgactta gatcatgtta
    10561 aaaaagttga attttcaagc aaagaacagg aattgaaaaa aatccaaaac gaaatgggtg
    10621 attcctggaa attgtttgaa ggtgacaata acccgttata tgatgtgtat gtagtaagtg
    10681 ctactgatcc ttcttataca aaacaagtag cagaagaagc agcagatttg aagaatgttt
    10741 ctcgttctga ttacggtgga gcttcctcag atcggatttt ccaaatcgct ggtgcagttc
    10801 ggacatgggg attagttgca gcggcattgc tgctgttcgt agcgatgttt ctcatttcga
    10861 acacgattcg gatcacgatt ttgtctagac agcgtgaaat tcaaatcatg cgtttagtcg
    10921 gtgcaaaaaa tggatatatt cgctggccgt ttttccttga aggtggctgg atcggtctga
    10981 tcggtgcgat tcttccgatt ttgatcatca catttggtta tgcatggttt ttccaattga
    11041 tcaatccatc attattacgt tcccattatt cattgattca tccacaaaat atcgtttgga
    11101 aaatcaatct tttaatggtc ggcattggtg tgatcattgg ttcacttggc tcgatcattt
    11161 ctatgagacg tttcttgaaa ttctaatacc acaatataaa taaaatgaga aagttggaca
    11221 gtttcgttcc agctttcttt ttttattggc taggagaata gaaggaagcg gcttttttcc
    11281 ctttacttta aaaaacaact tgggtattct taagttggta gaagaagagg tgaagccgtg
    11341 aaaattatcg ttgacaattt ttttacgatc cttttgatta tgaatatttt gctttccttt
    11401 atcatcgttt ttcgagaaag aaaggaaact gctcaaacat gggcatggct gctcgttttg
    11461 atgttcattc cagttgtagg atttatttta tatatttttt taggaagagg gatttccaaa
    11521 gagaaaatct ttgatttgaa gatccaaggg aaaatcggta agaacctgga aattgaagaa
    11581 gacaaacagg cattgatgcg agggctttat cctcatccgc ctactggaca agtagatgtg
    11641 aaacaattga tctatatgct cacggtattt gaaagtacgc tctatacgac taaaaatgaa
    11701 atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc tgattgaaga tatcaagcag
    11761 gcagaagacc atatccactt ccagtattat atttatcgca gtgatgcatt aggtgaagaa
    11821 gttcgagatg cattgacaga ggcggcaaga cgaggggtaa aagtcagagt ccttcttgat
    11881 gcatggggtt ccacacaagt ttcttccagc ttttttcaga atttgaaaaa agcaggcgga
    11941 gaagtcgcat ttttctttcc tctatttgtt ccgtatatta atcctcggat caattatcgc
    12001 aatcatcgta aaatcgtagt tattgatggg aaaatcggat atacaggtgg gttcaatgtt
    12061 ggaaatgaat atctaggatt ggtaaagaaa tttggttatt ggcgagacaa tcatttgcgt
    12121 atttacggag aagcggtgta taccttgcaa aaccgttttt tgatggactg gaattctcag
    12181 catacaaaag aagtaggcta ttctcccaaa ttttttccgt ccattcacac tactggtgat
    12241 atagctgttc aaatcgtgac aagcggacca gataccgaac atgaacaaat caaaatgact
    12301 tatttgaaaa tgatttcgtt agccaaacgg gagatattga tccaaacgcc ttattacata
    12361 ccggatggat cgattcatga agcactgaag ttggctcttc tttcaggcgt aaaagttcat
    12421 atccaaatac ctaacaaacc agatcatctt ctcgtttact gggcaacgta ttctttcgct
    12481 gcagagctga tcgagtatgg agcaaggatc gaaacatatg agaatggatt tattcatgca
    12541 aagacgatga ttatcgatgg tggcattgtt tcagttggct cagcaaatat cgatgttcgt
    12601 tcatttcgct tggactttga ggtcaataca ctggtttatg atgaacgaat ggcttcacgt
    12661 gtaagaaaag ctttttttga agactcgaaa atttctacgc atctcacaaa agaaatgtat
    12721 gaaaaccgag gaataatcat caagatgaaa gaaggattag caagattgat ttcgccgctc
    12781 ctttaaaaca taaaaaggac atgagaacca tcaacagatg gagatctcgt gtccttttct
    12841 aacggggcca tttctgagag tacgtcagaa atggagaaag cgtctcgcat gagcgaaatt
    12901 aactttgggg tatctcttgt tttttgggga aagcaagaaa taagctaata tatgctatta
    12961 aaagagacac atttcaaagc cagtatagca tagaaacttt taatgagata gtttttttta
    13021 caattatgag aatttatttt gttatttaat gattttgatt cgttttcatc aagtaatgaa
    13081 aatgataaaa taaacatact ttaggattaa aggagatttc taatgaaaaa acttaaatta
    13141 ttcttaccaa taataggatt agccattctt ttatcaggct gttcaaagtg gattgatcga
    13201 ggagaatcca ttacggctgt tggctcttct gctttacagc ctttagtaga aacagttgct
    13261 gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg tacaaggggg cggaagcgga
    13321 actggtttgt ctcaagtaca gtcaggagca gtcgacatcg gaaactctga tttgtttgcg
    13381 gaagaaaaat cggggatcga tgcctcagca ttagtcgatc atcgagtagc agttgttggg
    13441 atcactccta ttgtgaacaa ggatattggc gtaaaagaca tcagtatgga aaacctgaaa
    13501 aaactattta caggcgagat caaaaactgg agtgaagtcg gtggaaaaga tcagccagta
    13561 gttatattga accgcgcttc aggaagcgga acccgtagca cgtttgaaaa atgggtattg
    13621 gacggagaaa cagctgttca ggcacaagaa caagattcaa gcggaatggt gcgccagatc
    13681 gttgccgaca cacccggtgc aatcagctat gttgcttttt cttatgtttc cgatgatgta
    13741 gatacgttga atgttaacgg tgtagaaccg acagatgaaa atgtgacaac gaacaaatgg
    13801 acgatttggg catatgagca tatgtacaca agagatcatc caaaaaaact gacaaaagaa
    13861 tttttagact atatcttatc tgatgaagta caagataaca tcgttggaga actaggttat
    13921 atccctgttt ctcatatgaa agtcgaacgt gattggcaag gaaatatcgt taaagagtaa
    13981 tctttacaca aaatttacaa acttagctaa acttttacat gttattaaaa ccaaattgat
    14041 gttcaatttg tacactgagt gagtatgtta aacctgatta agggggagtc atctgtggaa
    14101 gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa aaatggaaca gcgtggaaaa
    14161 ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg tattatctat tttttatttt
    14221 gtagctagta aaggattggc cacctttttt actgataaga tcaatgtatt tgattttctg
    14281 tttggtacag attggaatcc aagccaaatc ggagcaaatg ggaaaccaat ggtaggggca
    14341 ttgcctatga tcacaggttc gtttattgtg acctttttat ctgctatcgt tgccacgcca
    14401 tttgccattg gtgcagcagt attcatgacg gagatctctc ctaaaaacgg aacgaagatc
    14461 ttacagccgg tcatcgagct gcttgtaggt attccttctg tcgtttatgg ttttatcggt
    14521 ttatcagtta tcgtgccttt tgtcagaagt atttttggcg ggacagggtt tggtatctta
    14581 gctggtacat ttgtgttgtt cgttatgatc ttgccgactg tcacaacgat gactgttgat
    14641 gcattaaagg cagtgccacg gcactatcgg gaagcttcat tagcgcttgg tgccacaaga
    14701 tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc ccgggatttt aacagcagtc
    14761 gtttttggta tggctcgtgc gtttggtgaa gccttagcta tccaaatggt catcggtaat
    14821 gcagcattga tgccaacagg acttactact cctgcttcta cattgacgtc catcttaaca
    14881 atgggaatcg gaaatacaat catgggaaca gttgaaaata atgtgttatg gtcattagcg
    14941 ctgatcttgc tattgatgtc attattcttt aatatcgtga tccgtatgat tgggaagaaa
    15001 ggagagctga aataatgaac gcaaaacgct cagataaaat tgctacaggt gttttatata
    15061 tagtttcggg gatcatcgta ttgattttag cagctttact actttatatc ttagttcggg
    15121 gaataccgca tatttcatgg tccttcttga ccgaaccttc caaagcctac caagaaggcg
    15181 gtggaatcgg gatccagcta tttaactcga tttatttgct tttgatcacg atgatcatca
    15241 gttttcctat ttctttaggt gcgggtatct atctttcaga atatgctgga aaaaactggc
    15301 taacagatgt gattcgcacg tcgattgaaa tcttgagctc gttgccttct gtcgttgtcg
    15361 gtttattcgg tttcttggtt ttcgttattc aattccagta tggtttctcg attatttctg
    15421 gtgcattggc tttgactgta ttcaacttgc ctcttttgac tagaacagtt gaggagtctt
    15481 tacaagcggt ccattacaca caacgagaag caggattggc cttaggtttg tcacgctggg
    15541 aaacagtgat aaaagtggtg gttccagaag cgacaccagg gattttgact ggtgtgatcc
    15601 taagttctgg aagaattttt ggtgaagcag ctgcattgat ctatacggct ggacaaagtg
    15661 cacctgcgtt agattttacg aactggaatc cattaagtgt ttcaagcccg atcagcattt
    15721 tccgtcaagc tgaaacatta gctgtccata tttggaagat caatacagaa ggcacgatgc
    15781 ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt gatcattgct gttttactgt
    15841 tcaacttcgg cgctcgtgcg attggcaaac gattataccg aaaaatgact tcagcctaag
    15901 gagaagaagc aaaatgaaaa aatataactg gaatgaaaca aatgtcatca ccatgccaaa
    15961 agagaaagaa gtagcactat atacggatga tctacatgtc tggtatggcg acaatgaagc
    16021 aatcaaagga gtggatctgg aatttgaaaa aaataagatc actgcactga ttggtccttc
    16081 cggctgcgga aaatctacgt atcttcgttc gttgaatcga atgaatgatg ggatcgctaa
    16141 tacgaaaatc actgggaaaa tcatgtatca aggcgtagat attaatgcgc ctcaagtgga
    16201 tgtttatgag atgcgcaaac ggattggcat ggtttttcaa cgtcctaatc cgttcagcaa
    16261 gtcgatttat gacaatatta catttgcttt gaaacagcac ggcgaaaaaa acaaaaagaa
    16321 attagatgaa atcgttgaaa caagcttgaa acaagctgcc ttgtgggacc aagtaaaaga
    16381 cagcttggac aaaagtgcct tggctttatc tggcgggcaa cagcaacgtt tatgtattgc
    16441 tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac gagccggcaa gtgctttgga
    16501 ccctatttcg acaggaacgg ttgaagaaac acttgtaaac ttgaaagaag actatacgat
    16561 catcatcgtg acgcataaca tgcaacaagc agctcgtatc agcgactata ctgccttttt
    16621 ctatctagga aaagtaatgg aatatgacaa aactaacaag atatttacca gaccaaaaat
    16681 tcaggcaacc gaagattatg tgtccggtca ttttggttaa gacggaggcc tcaacatgac
    16741 aaaagaaatt atttcatcta aagatcttca tctgtattat ggaaagaaag aagcactaaa
    16801 agggatcaat ctaagtttca atcaagggga aatcactgcg atgatcggtc cttctggttg
    16861 cgggaaatcc acttatctgc gttctttgaa ccggatgaat gacctgattc cgaatgtaac
    16921 gatcaccgga agtgttttat ataaagacaa agacatctat ggtccaaaaa cagatgttgt
    16981 tgaactaaga aaagaaatcg gcatggtatt ccagcagcca aacccatttc ctttttctat
    17041 ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa aaagataaaa aagtccttga
    17101 tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg gatgatgtga aagacaaatt
    17161 gcataaaagt gctttatctt tatccggtgg gcagcagcag cgtgtatgta tcgcacgtgt
    17221 tttggcagtt aatccggaaa tcatcttatt ggatgaacca acaagtgcgc ttgatcctgt
    17281 ttctactggg aaaatcgaaa gtatgctgtt agaattaaga gagcggtata cgatgatcat
    17341 cgtgacccat aatatgtctc aagcttcacg tatttcagat aagacggcat tctttttaga
    17401 tggtcacttg atcgaataca acgatacgaa gaagatattc atgaatccag aaaaacaaga
    17461 gacagaagat tatatttcag gtcgatttgg ataaggagga aacggaatgc tacgtacaca
    17521 atttgaagaa gaattgctga atctccataa tcagttctac gaaatgggga tgatggtgag
    17581 cagcgccgtg cacaagtcag ttcgtgcata catcaaacac gataaaatct tagcccaaga
    17641 agttatcgaa aacgatataa atatcaataa catggaaaca cgcttggaga agaaaagttt
    17701 tgaaatgatc gcattgcaac agccagttac aactgatttg cgtatgatca ttacggtcat
    17761 gaaagctagc tcggatttag aacgtatggc cgatcatgcg gtgtctgttg ctaaatccac
    17821 gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa aaagagatct ccgatatgtc
    17881 cgattatgtg aaaaaaatgg tggacaacgt cctagtagct tatgtcaaaa cagacgaaaa
    17941 agacgcacgt acgatcgcca atatggatca aagagtcaat gactatttca atcgaatcta
    18001 taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca gtcatcagtg gaacagacta
    18061 cttgaatgtt gccagttatc tagaacggat cggagactat gtgacaaaca tctgtgaatg
    18121 gatcgtctac ttggctacag ggaaaatcac tgagttaaat acgaaccaca atgagatttt
    18181 ctaaactgaa gctgtgatta tgccgtttgt ttaggaaaag aggttgtgaa atcagcggtt
    18241 gtgaaaaagc tgttctgcat caagtaataa gaacagtcaa acaaaaaata gctcctcata
    18301 tttttcgttt ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattcggg
    18361 aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc atttcataga aaggataaag
    18421 aatgcaaaaa aaaaattagc atcatcacta tattttttat ttgtgtgctt acttcatggg
    18481 ttattcagca tcaagctgct ttatatgaag tacctgtcat cgagatcgaa aaagtacgaa
    18541 taaaagagga tcatcaattg attacaggaa gactgatgaa cacggatcaa aaaggagaat
    18601 cgatttattt atcggttcct tatcaaaaaa atcaaataga aacagcaaat ataaaagcga
    18661 gacaaatgta tctagtccaa atgcatggag atgaatggca gctagtagca aaaaaaaatg
    18721 atggctggct tttcttcttc acaagtatct tcattggcgc catgttgctt attggcggaa
    18781 aacagggtct atttgcttta ggaagtctca tcatcaatat cgttttactt tacttttgtt
    18841 gctcatcttt actaaaacag aagggatttc tctattaaat atatctatcc tgttcgtcgt
    18901 tatagggatg attattagca tattggcttt aaatggatgg aatagaagca gtataatcaa
    18961 aatttcagct gcagttacga gtgttttttt agcatttttt atttgtcttt tgacaatgaa
    19021 tcattggaaa gaccaaggac tgcgctatga ggaactgaat tatctgacac gaccgtatcg
    19081 ttctgttttt ctttctagcg tactgattgg aacagctgga ggaacactcg ataccgttat
    19141 cacagtcatt gcgacattag aagaattgac taagcgacaa ccagaaataa cagcgaaaca
    19201 gttatggctt tcaggaaggc aagtgggtcg agatgtgatt ggctcgatga ccaatatcct
    19261 tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt ttatatttag gaaatcaatg
    19321 gtcgttcaaa gaaacgatag aacagcattt atcattggaa tttttacgcg tcattgctgg
    19381 cagcttagga atagtattat cgatccctat ctcggtcctc ttttttctga ctgtaaggag
    19441 gctgaaacga tgaatgtact tattatactt gttgggattt tagcaatcag cgtgctactt
    19501 attggcggta cgcaaggtat gtatattctt ttaggcttat tcatcaattt agggatcttt
    19561 ttcctgttgt tattcggtta tcatcagaag tggccgatac tggcattgtc ggtcatagga
    19621 tttttattga ttgctgtggt tatcttattt tttatcaacg gttataatct aaaaatgcgt
    19681 gcagcttttg tgagtatact gatcttttta ttttgtttcc tactattgct tcctatcact
    19741 gattttttag cgatccaagg ttttacatcg gtagaactag aagaattatc tggcttagat
    19801 aagactttag cgattgattt tagattactt acgagatcgt tgcttctgat tagtcttagt
    19861 ggtgctgttt tagatgctag tgtagcaatt tctagtggga cattcgaagt atatcaggca
    19921 aatccgcaat tatcttttta ccaattgcgc cattctagtt tcgctattgc taaaaaaata
    19981 ttggcgtcaa cagtaatgac acttcttttt gcattcatgg gaagttcttt agcgttgatc
    20041 ctctggttta ttgatttaga cattcctttt cagcaagtga tcaatgaaaa atcatttgtt
    20101 ctagaatata cgatggctat tcttaccaca ttgtctgcac tacttgttct tcctttaacc
    20161 tgcgtgacta cagcatatct ctttacaaaa aagaaagaac aactcaagat ggagtaaaag
    20221 ttacaactta agtcctatga catttttact gaatcagtgc ataatatgga tgtaaggaaa
    20281 agcttgcaaa caattttgct taaggaggca atttccatga aagaaagaga acgtatttta
    20341 gatttagtga aaaaaggaat cctttcgact gaagaaggct tagatttatt agaaagcatg
    20401 gcaactgaaa aagacgaaaa gcaaataaaa aaagaagcag acaaggtaac tgctagtcat
    20461 aaagaaaaag atcaggcaag tcaattgatc gataaacttg aaaacggcga agaagaaatt
    20521 tctgagccag ttgatccaaa ggaaagagaa agacaagatc aagaaaactt agaaaagatc
    20581 ttagatgaac tagcaactga agcgaataaa acttcggcgc gtctagatga agtaaatgct
    20641 caattcgctg acaataaggc agcacgcaat gagaaacagg aagcattgat gcagctgaat
    20701 acaaaagaag aattaggcga gttgacagaa gaagaattag cacagcgtca gacgttagaa
    20761 gcagaaatca aagaattaga agctagtggc gatacactgt tagaagaaca attgaaatta
    20821 gaagcagaat taaaagatat ccgtaagaat caatggtctg agaaaaaaga aacgtttact
    20881 gataagtttg aattgccaga tgattggaaa gatcaagcaa cagatacact gaatcaagta
    20941 ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa aacattccaa
    21001 actgtttcag aaacagtgaa tgacaacatg gaatggaaag atgtcagctt acgtgtccct
    21061 gggattgcga caacgaaatt tgagcatgaa ttttactatg aagctcctgc agcaagtatc
    21121 cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag caacgatgtg
    21181 aaagttgaag ctaagatcaa actttacgga aaaatgggtg ctgaaccatt tgaagcattc
    21241 tctgaacgga gccagattga ggttaatgaa gatcatatct ccttccaaat tccaaataaa
    21301 cgtgtgcgtg cagaccttgt tttctactta cctaaacgag tatacgacca cgcagctatt
    21361 aaacttctaa atggcaacat catgattgaa acattggaag ctaaagatat ttatacaaaa
    21421 tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga agtagaaggt
    21481 gtaaacggca atatcgatat tcgaaacggg aacatcttag attctattat cgaaacagta
    21541 aatggtacag tgacatttgg tgcgacagca gaaaaccttt ctgtgtcttt ggtaaatggt
    21601 gatgtacgtc ttacgattaa agaagataat ttgaaaaaag tagaagcaag ttcagtgaac
    21661 ggaaatgtca aagtcgcttt accagatggc atcggtttgg aaggacatgc taaaacaagt
    21721 cttggaagta tcaacagccg tctgtccaat tacgaagtag ttcgtgagaa aaaagaacgg
    21781 acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg aaatcacgca agtgcaactt
    21841 tcaacgacaa caggaagtat ctatttaaaa gatacagata aataaagagg ttgtgaaaga
    21901 gatgttcagc cttgagcaat aagaagcgat tttgtaaaat agcttcccct attttacaaa
    21961 attgtgcctt attgccgata ggctatctct tgaacaccgt ttaccaagag attatgaacc
    22021 tgccaattat cgccgagtca caaggaataa tttcagaaaa agctcgtcac gttttatgaa
    22081 tatggtaatt tgtgaccgag aattttgcag aaagacaccg attaagaaga aggttgtcac
    22141 aacgattttc ttcatttaaa atagtagaac agaggtgaga catgatgaaa aaaaagttga
    22201 caaaatccaa taaaaatgtt gttttgacag ggactttagc aggaatagga gaatacatcg
    22261 ggattgatcc gactgttatc cgtgtgcttt atgtattcct gagcttagtt gcttttggaa
    22321 gtccaatcat tttatacatt ttattagctt tgattatccc atctgcgcgt tctgcacgaa
    22381 caaactatgg acataacaat gcatattatg aaaaaaacaa ttatcgtgaa caaactcgtg
    22441 aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga tgacgaatgg agtgactttt
    22501 aatgtcgtac tttcaacgta ttttagttaa tacgttaaca ttcatatcat tggctgttct
    22561 ttttcctgcg atggtccatg tgagcagtat ttggacggcc gttcttgcca gcttcgtatt
    22621 gtctgtgttg aatatgtttg tcaaaccgat tttgacgata ctgtccctgc cctttacatt
    22681 agtcacattg ggattgttta cgtttgttat caatgcagcg attcttaaaa tgacctcctt
    22741 ttttgtggga gcagaacatt ttagtttttc ttcattcggt gcagcgttgt tagtagctat
    22801 cttgatgtcg cttgtcaatg taattgtcag cgaacataac agtagaaaat aaaagaaagc
    22861 tgtgccgaac tatcggcgca gtttttttat ttagaatcag gaatgcgttt tctattacat
    22921 tggtaattaa ataaaaacgt tttttttaat tggtagtact tttggctaga caatgataaa
    22981 atgatagaga acactctaat agaaatgagg tagcatgaat gtcagaagtt gtaaaagtta
    23041 gccaattagt gaaaaaatta tctttggaaa tcgtgacagg cgacgagaaa agcttagacc
    23101 gtgtcatcgt aactggggac atctcacgcc caggactaga gttgactggt tattttaatt
    23161 attattccca taatcgttta cagctatttg gcagtaaaga aatcagtttt gcggaacgga
    23221 tgatcccaga agagcgtttg atcatcatga gaagattatg tagtgaagat atgcccgcat
    23281 ttattatttc aagaggatta ccggctccag aagaaatgat ccaggcagca aatgaaaatg
    23341 ggatagctgt actgcgttct cccatctcaa cttctcgttt attaggagaa ttatccagtt
    23401 atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg tgttttagtg gatgtttacg
    23461 gattaggtgt actgattcaa ggtgacagtg ggataggaaa aagtgaaaca gcgttggagt
    23521 tgatcaaacg cggtcatcga ttgatcgcag acgatcgagt agatgtttat cagcaagatg
    23581 aattaacggt gatcggagaa ccgcctaaaa tcctggaaca tctaattgaa atccgaggaa
    23641 tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc ggtacgtggc tctatgcaag
    23701 tccagttggc tgtttatctg gaagcatggg caaaagataa gaaatacgac cgtctgggct
    23761 ctgaagacac gaccgttgaa atcgcggaag tgggcattcc acaaataaaa atccctgtca
    23821 aaacaggacg aaacgttgca atcatcattg aagtagccgc gatgaatttc cgtgcacgta
    23881 caatgggctt tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg attgaagaaa
    23941 actcaggcaa cgactagcaa ggagtgacaa tatgttagca caaatcaatc gagttgcttt
    24001 tgagcttgta gggattccta tctactggta tgcattgatc atcgtatcag gtatcattat
    24061 cgccatgtgg cttagcagca gagaggctgt tcgggtagga ttaaaagcag atgatgtgac
    24121 agattttatg ttgtggggat tgcctatctc cattattggc gctcgcttat attatgtttt
    24181 atttgatctt ccccagtata tagctgatcc aatccaaata ttcaatatcc gttcaggagg
    24241 cttagctatt tatggcggac tgattgcagg cgcgatcacg ctgtattttt ttactaaata
    24301 tcattttatt tctttgtgga cttttttaga tatcgctgca ccaagtgtat tattagcaca
    24361 ggcaatcggt cgttggggaa atttcatgaa ccacgaagca tatggaccag acacgacgcg
    24421 aagcttctta gaaagtctgc atttaccgca attcatcatt gataatatgt atatcgaagg
    24481 gatctatcgt caaccaacat ttctttatga atcactttgg agtttgattg gatttatttt
    24541 gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa ggcgaagtcc ttttatgtta
    24601 tctaatgtgg tatagtttcg ggcgtttctt tattgaagga ttacgtacag acagtcttta
    24661 ctttttaggt gtgatccgta tttctcaatt attatctgct gtgttatttg tcggtacatt
    24721 gattctattt atttggcgca gaaaaaaagg aaatttaccg gattatgacc gctcattagg
    24781 aaaaaatcaa caaattatct gatttttaga gcgttaaaaa tgaaaaaaat ttcaggagtg
    24841 gtaaaatgaa ttatagagta gcggttttag gaccaggctc ttggggtact gctttggcac
    24901 aagtcctcgc cgaaaacgga catgacgttc ggatttgggg acatcgtgct tcacaagtag
    24961 aagaaatcaa cacccaacac acgaatcaac gttatttacc agatcattat ctacccgatt
    25021 ccatccaagc attcaccaat atggaagatg cagtaaaaga cgcagatgcg gtgctattcg
    25081 tcattccaac gaaagcaata cgttctgttg caaaagaact agtgcctaaa ttgaatacaa
    25141 agccagtgat catccatgca agtaaaggat tagaacaggg aacacataaa aggatctctg
    25201 acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc tgttgttgtt ctttctggac
    25261 caagccatgc agaagaagta gcgaaacatg acatcactac gatcacagct gcaagtacag
    25321 atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa taattatttt cggatctata
    25381 ccaatcctga tgtgatcggt gtagagatgg gggcagcttt gaaaaatatt attgctattg
    25441 gtgctggcgc tattcacggc ttagggtttg gcgataatgc aaaggcagca atcatgacgc
    25501 gcggattagc tgagatcagc cgtttaggtg tagcaatggg tgccaacccg ctgaccttta
    25561 tcggattaag cggagtaggc gatctagtcg ttacttgcac aagtgttcat tctaggaact
    25621 ggcgcgcagg caacttgtta ggacaagggc agcccctaga agaggtgcta gccaatatgg
    25681 gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat ggaactagcc caaataatgg
    25741 aaatcgaaat gcccatcact caaacaatct atgaagtgct gtatgaacaa aaagaaatca
    25801 aaacagccgc aaaagaaatc atgttacgtg atgggaaaaa agaaaatgaa ttctcattaa
    25861 caattttttg aaggagcgaa tgtatatgaa agtcaaaaaa gcagttattc cagcagcagg
    25921 attaggaaca agatttttac cagcaacaaa agctatggca aaggaaatgt tgccaattgt
    25981 tgataaacca acaatccagt tcatcgttga agaagcgttg aaatcaggaa tcgaagacat
    26041 cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat cactttgatt caaatattga
    26101 attagaaaca aacctaagtg aaaaaggaaa gaccgaatta ttgaaattag tagaagaaac
    26161 gacagatgtc aacttgcatt tcatccgtca atctcatcca aagggtttag gacatgccgt
    26221 tcttcaagca agagccttta ttggaaatga accgtttgtc gttatgctag gcgatgatat
    26281 catggaagat gaagtaccat tgacacagca attaatcgat gattacgaac gtactcatgc
    26341 ttctacgatc gccgttatgc aagttcctca tgatgaaact tcaaaatacg gaattatcaa
    26401 tccaggagaa gtattggaag atgggttata caatgtcaaa aactttgttg aaaaaccaga
    26461 tccaagcaaa gcaccaagtg atttagctat tattggacgt tacttattga ctccagaaat
    26521 ttttgatgta ttgtccgagc aacaaccagg agccggaaat gaaatccaac taactgacgc
    26581 aatcgataca ttgaataaaa cacaacgcgt atttgcccgt aaattcacag gtaaacgtta
    26641 cgatgtcggt gacaaatttg gttttatgaa aacaagtatt gaatatggtc taacacatcc
    26701 agaagtaggt gcacctttac gtgagtatat cctgtcttta ggtaaaacat tagaagcaga
    26761 aggaagcaag atcgatcata atccaaaagg aataacagaa atcaaagaag aaaagaaaaa
    26821 ataacccttt tttctaacga gcttcagctg ctaattgaat aaaaagtagt tgaagctcgt
    26881 ttttatttgt ttaaaaaaca cgaaaatgct atccttatag aaaagagaga caagaaggga
    26941 gtttgatcat gtcaggagga gaaattgcag gattgattgc agcaatcgcc tttgcgatac
    27001 ttgtcgtttt tatcgttcgt gtgttgttgc aagtttctaa aactttagaa aaagtagatc
    27061 agactgtagc agaagcaaat acgacgatcg aaatcgtcac aaaagacgta gatcttttgt
    27121 ctagacaagt agaaggcttg ctggtcaaaa gtaatgaact gctcttggat atcaatggaa
    27181 aagtcgcaac gatcgatcca ttgttcacag cagttgctga tttaagtgaa agtgtatcag
    27241 aacttaatca ttctagtaaa aacatcgcga cgaaagtcgg cggaatcggt aaagcgacag
    27301 ctaaagcgac ggttgccgga aaagtcggtg ggacagcgat gaaatttttt aaaaacaaaa
    27361 aacatactga aacgacagga ggaaaataaa aatggctaaa aaaggtggat ttttattagg
    27421 tgcagtagtc ggaggaacag ccgcagcaat cgccgcgtta ttgttagcac caaaatctgg
    27481 taaagaactt cgggatgaat tagctgccca agcggatgat ttgatggata tggcttcaga
    27541 ttacacagat atggcgaaag aaaaatcgac tgagttaaca ggtattgcaa aagaaaaagc
    27601 gcaagatctt tctcaacaag caggtgatct agcaggtagt gtcaaacaaa aagtaagtga
    27661 atcagcagat gaagcaggaa gtgaatctga cgatatcttg ttaaatctta aagaacaatc
    27721 agctgactta tctaatcgtt tcaaaaaaac ggcagaagat atgaaaaaac ctgcagatga
    27781 attaggaagt attgctaaag atacttcaga agatatcatg attgatgtaa aagacgctgc
    27841 tggtaaagca ggagatactg tcaatgaagg aatgtcagaa gcaaaaccag ttgcagatga
    27901 cacaaaagaa atgatgaaaa tggcaaaaga agatgcgaaa gaagctaaaa aagaaataaa
    27961 agatacaatg acagattcat ctacaaatca ctaaaatcac aaatttatag aataaaaagg
    28021 tgggagcgga ctagctctca ccttttttac tcttattttt ctaaaacaag taattctttt
    28081 ggtgtagtcg tgaatggcac acagccatcg cttgtcacat atacacagtc ttcgatacgt
    28141 acccctactt tttcaggaat atagattcct ggttcaagag agaagcacat cccttcttcg
    28201 attactaagt catttccttc tacaagggaa gggaattcgt ggacggttgt tccgatcccg
    28261 tgacctaaac ggtgattgaa atattcgccg tagccatagc tgttgatcac gtttcgtgcg
    28321 atttgatcaa gttcacctgc tgtcacacct ggacggacgg cttcttgtgc ggcaagctgt
    28381 gcttcaagag tgatgttgta tattttttct tggaagtcgg ttggtttttg ataagcaatt
    28441 gtacgagtag catcactgca atagccattc caaacaacgc caagatcgaa taacacaagt
    28501 tcattcggtg aaatcttcgt gtttcctggt gtaccatgag ggcttgcagc atttgctcct
    28561 gcaaggacta atgtgtcgaa agacatttgt gaaacacctt gttttttcaa ttggtattcg
    28621 atttctgcaa tgatcgcttg ttcagtcacg ccttctttca ctgctttaaa gccaatctca
    28681 aatgcaacat cggcccattt accagcttcg atcagtttgt ctttttcctc tagagttttg
    28741 atcagttgta gacgttggat gatggaagtt agatcagtgt aagaatccgt tcccgtcaag
    28801 atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt tcttttcaat ccctacattg
    28861 ccaggctgac catagcgagc acgaagttct tttccgatga tctcccaagg gttttcgctg
    28921 tctaaatagc cacggacatc atacgcccaa ctactgtttt gagcatcttc aacttccaaa
    28981 gcaggagtga aaaggaaagg atcttgttca agagggataa aaagagctag tacacgctca
    29041 tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc cagggtcgct gatataagcc
    29101 aattcgactt ggtgttgtgc catccattga cgtagttcat tgatttttac gttattcatt
    29161 tggcaaccta ctttctaaat taattttgta ttcaataatt gtaccactga tttattaaaa
    29221 gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat gaaaacagag ataaaacggt
    29281 tgcaattctt gagattattt gatattctat tttagaaatg taagacgtat tttcagaaaa
    29341 catataaaat atgaatagga gaacacaaat ggaaaagcaa acaatcacta tctatgatgt
    29401 agcgagagag gcaaatgttt cgatggctac tgtttcacgt gtcgtaaatg gcaatccaaa
    29461 cgtcaaacca gcaacaagaa aaaaagtctt ggaagttatc gatcgcttgg attatcgtcc
    29521 aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca acagttggtg taatcatccc
    29581 tgatgtcagc aatatgttct ttgcttcttt agcacgtggt atcgatgatg tggcaacaat
    29641 gtacaaatac aatattatct tagcaaattc agatggcaac gaccaaaaag aagtcaatgt
    29701 attgaataac ttactggcta agcaagtgga cggcgtgatc ttcatgggtc accatatcac
    29761 agacgaaatc cgtggagaat tttcacgttc aaaaactcct gttgtattag ctggctctat
    29821 tgatccagat gaacaagtag gtagcgtaaa cattgattat gtatccgcaa caaaagatgc
    29881 agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct tttgtcagtg gtgcgttgat
    29941 cgatcccatc aatggacaaa atcgtctgaa aggctataaa gaagcattaa gcgaaaataa
    30001 cctaccatat agcgaaggat tgatctttga agcacaatac aacttcaaag atggtctatc
    30061 tctagtcgat cggatccata atagcggtgc aacagctgtc tatgtttcag atgatgaatt
    30121 agccatcggt attttggatg gtttattaga ccgtggcgtg aaagtaccgg aagaattcga
    30181 aatcatcaca agtaacaatt cactactgac agaagtagct cgtcctcgtt taacaagtat
    30241 cacacaacca ttatacgatt taggggcggt atctatgcgc ctattgacga aaatgatgaa
    30301 taaagaagaa gtcgaagaaa aaacgattat cttgccttat ggaatcgaag aaaaaggatc
    30361 tacaaaataa taaaaggaca accattcaga ggttgtgtgc ctgccgttta actctgcgtt
    30421 acaaggagca atttcaaaaa acagcatctc atgtttgatg aatattgtga ctcgtgattc
    30481 ggagttggca ggagaacacc gtttatttgg gaaaaggggt tatgacaaaa ttttgttata
    30541 atcctttttt tgtgtcttaa aaaagagaga aagtggttga aattatattt atcgtatata
    30601 aaacaatccg tgtatagatg ctgtattttg ttcaccatca tcaaaaaaat aagggaatgt
    30661 gacaatacta ttgtcacatt cccttattac tgtgtattct attaaatcaa tcatctttct
    30721 catctgattc tttggttgct tgattcgctc ttgcataagc tgaagcggtg ttccagtaat
    30781 ctgtatagtt ttcatcggta ccgccaacgc caaacttaaa ggcgcttttc ttcggtccat
    30841 ttttcgccca tagcgattca actgtttttc ctggtgtttg gatggatttt ttattgacag
    30901 taacttttcc aggtgtttga ccagtaaatc cagatacttt tttcttcatc acatcagatg
    30961 ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt tgcttggtaa gcttgattga
    31021 tcagattggc aagataatta gccgaacgta tccttgcttt gctgtccatt ccagtattgt
    31081 catcgtgccc tgcccagcta ctgatggtaa tagacggagt agagacaatt agccatgaat
    31141 ctcggtattc attggtagaa ccagtttttc cgacccaatc tgcttttcct agatttccgt
    31201 ttaagcttga gatggcatct ttgaatggtg tagtgatttt tgcattgatc acactgcgca
    31261 tcatgtcatt catgatcgat gccgttgctt ccgaatagat ccggacagag ttgctttcgt
    31321 gtttataaat tacatttcct gcgttatctg tgatggaatc gatgatatag ccttcttcat
    31381 agacgccgcc gtttgctaaa gcctgaaatc cgttggtctg ttgaagcgtc gtgacatcta
    31441 ctgtacctaa aggagcagat tcaacgcccc aattgtcatt tgccgggtag ttcatctttg
    31501 acaagtagtg ctcgtaagca tattgtttcg atccaccgga attcaacaca tcttgatata
    31561 agtgataagc cgggatattg ttggaccatt ctagtgactc acggacggtt tggaaggtgt
    31621 tcgatccttc gttagtagca ttgacgattt tctcgccagc attttctcct tcttgccaag
    31681 ttgttgcata atctgataca cgggattctg atccgataag ccccatgtcg attgcaggtc
    31741 cgtaaacaag aactggtttg attgatgagc cagcttgacg ttctgtatcg aaggcatggt
    31801 tattttgatt ctgactgtaa ttccgtccgc caacgaatcc gtagatgcgg ccagttctgt
    31861 tatccatcaa tacatttccg gtttctactt gactgccagc accatcatct aacatgtatc
    31921 cataattttc aacgccattt tgcaatgcag catagacatc tttatcgatt gtcgaatgga
    31981 ttgtatatcc tttattttgg atcgtttgct gtgccttttg ataataagcg tcactgatcg
    32041 atgaatcaga catatcagcg ttgtcttttt cagccaattg ttgtgcaatg attcttgttg
    32101 cttcattcat cacagtataa tacaagaatt ctctgtcagt ctgttctgcg atttgttgtg
    32161 gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc gtattgttct ttcgtaattt
    32221 ggccttcacg atacatactg aataaaacaa aatcttttct ggcaaggccg gcagaaagat
    32281 cttcttttag cgcacctgtg ttcgtgtatg gactatacgt gatcgggctt tggggtaatc
    32341 cagcgatata agcagcttgc ggcagagtaa catcttttgc atctacacca aagattcctc
    32401 ttgcagcttc ctggactcca gcaatgtttt gtcccttatt attacgacca aatggtgaga
    32461 cattcaaata cgtagcaatg atctcatctt ttgaaaagta tttttctact tgtgcagaaa
    32521 gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc tgtaaggatc tgctgtttga
    32581 ctaattgctg tgtcaaagtt gatccaccag aagagccaat accagtagct tcagatacta
    32641 atgctcgtaa aacagcttta ggaacgtaac cttggtgttt atcgaagtat tcatcctctg
    32701 tgctgataat tgcagttttc aataaaggtg aaattttatc agatgaaata gtcgtgcgca
    32761 tcagatctgt ctggattttt gatatctctg tattatctgc ataaacaatc tttgacgttt
    32821 ctgtaatgtt tcctagttct gtctgcagtg cttttttagt cggcaattgt gtgtcttcga
    32881 cgagataggc aaaatagcca gcgcctattc caatacctag agctgcgaac atcaaaagaa
    32941 gaacagtaat aaaaacagtc aatgattgaa aaactctaaa aataatctta ggaacaaacc
    33001 atttttcttt ttttcttcta ttctgttttt ttcgagtatt tttgcgtggc aaaagggtca
    33061 cctcaactaa tggttttatt tcgctaatta tagcaaataa tagaacaaaa aaacagacaa
    33121 ggctgttttt ggcaggaatt tcttttttaa aggagttaaa acgtgagaaa atgatctttt
    33181 ttataaaaga gttcttaagc ttttctttag agaaaatttc agttactatg gagaacaggg
    33241 caaagaaaaa tcaatcattt tctttgtata tcatcttacc aatatatagg aattgttagt
    33301 aacaattgcc tttcaaggag gaatccatcc atgttattag gctcacacgt cagcatgagc
    33361 ggaaagaaaa tgctgttagg agcagcagaa gaagcagcca gttatggatc ttcgactttt
    33421 atgatctata caggcgcccc tcaaaacact agaagaaaag ccatcgaaga gatgaatatt
    33481 gatgccggaa aagcgtacat ggcagaaaac ggtctaacaa atatcgtcgt tcatgcccct
    33541 tatatcatta atttggggaa tacgatcaaa ccagaaatgt ttccttttgc cgtccaattt
    33601 ctgagagaag agattgttcg tgcggaagct ttaggcgcta ctcaaattac acttcaccca
    33661 ggtgctcatg taggggcagg aacagcagcc ggaattgcta gaattattga aggcttgaat
    33721 gaagttttga caaaagaaca gacggcacaa atcgctttgg agacgatggc aggtaaaggt
    33781 acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca tcgatggagt aaaattgaat
    33841 gaaaaattat ccgttacttt tgacacctgt catacgaatg atgccggtta taatattaaa
    33901 gaagactttt caggtgtaat ggaagagttt gataagatca tcgggttaga tcggcttaaa
    33961 gtactccata tcaatgattc taaaaatccg caaggttcgc ataaagaccg gcacgccaat
    34021 attggttttg gaacaatcgg attcgacacg ttgaataaga tcgtccatga tccaatgttt
    34081 gacaatattt cgaaaatctt ggaaactccc tatgtcggca aagataaaaa gaaagcttac
    34141 gcaccatatg gaaaagaaat cgccatgttc aaagcgcaaa cattcgatcc agaagtgttt
    34201 cctgagttat tagcagaaga agtgatactc taagattttc tatttgacag cagcaaaaaa
    34261 ggaccaggca cattgccttg tccttttttg tttaagaaac aaaggagtac tttttgcagt
    34321 ctttttcatc atgcatcttc tcccccctct ttttaaattt agacaaacag ggtatactgg
    34381 tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc aaaacgtgat tggaaggacg
    34441 aaaagatgct aaacacttta aaacccttga tcaaccaact gactaagaaa catacatact
    34501 ttattcttgg catagcccta ttatttacaa ttattcgttt gtatttatat agtaaagcaa
    34561 tctatggaac agacatgtcg gcaacctatg atgatcagtt gctgattcgc tatgctcaaa
    34621 atattttgga cggtaaatgg ctaggggagt atacgactac aacactttcc aaaggaatct
    34681 cctacagctt atttttagta cttgcacata aactgcattt ttcttatgca gttttattcg
    34741 gcatgttcag tatttttgcc agtatattgg ttgcactcgc gtttaaaccg atcgtgaaga
    34801 acaagtatct tcttttaagt atctattttt attttttgtt ttctccgatt agttttacac
    34861 atgaatattc cactcgaacg tatcgtaatg ccctcgtgat tccggctgtt gagatcatta
    34921 tcgcttgttt attagctatt tattaccgca aatacggttc tagcagaaaa ttgctgccat
    34981 ggtttttagg gctttctgtc gcttttcctt ttttttggtt cattcgcgaa gattctatct
    35041 ggcttcttcc ttttactgta gtagctttgg gtatcagttt cgtccagatc ttgctagggc
    35101 aagacttttc tttcaaagaa gtaaagaagt taaaaaaacg actctttctt ctctcgaaac
    35161 gggaatggct gaaactttca ttgtttttac ttcctatagt tggcttttcc atcgtctatc
    35221 agtggatcga atataaaaat gagacaacat acggtattgc aaccatcaat gaccgttcga
    35281 gtggagaatt tggacgtttg atgaaacaac tgatccgaat agatgatgaa acgaacttga
    35341 ataaagaaaa cagtgagata tgggtctcac atgctgcact aaataaagca ttagccgcat
    35401 ctcctacatt ccatactgtt tctacccgta ttgacgaatt atatacgact cacgcgtgga
    35461 ctcaaggtgg aaaagtaaaa gaattacccg gagatatcat cttttgggca ttgcgagatg
    35521 tagtaagcga atctggttat taccaaaaca atgcaaaaga aaccaatgat ttttggaaaa
    35581 aagtcaacca agaattagaa gaagcctatc acaagggaac catcaaaaag aaaaaagagt
    35641 tctatctgat gggatcagga gatggaaaga cgatgagtga tctgccaatc gtattcgatt
    35701 tccttcaatc aggatacgct tatgcatggc gatataaaga tttccatcaa ggaggaaatt
    35761 atagtttcgg aacgcaagaa caagtcaaac aggctcaaga attattaaac cttccgctaa
    35821 tggagaattg gatcgatagt aatgaaccga gaacacctaa cttccaatta acaaaatcag
    35881 caaaagtcgc gaatgtcatc ataaaaattt accaagttct ggctattccc gtgctgattg
    35941 cttcaatagc agggatatta cttatgatac taggaaccgt atttgcaaaa gctgatcgtg
    36001 cctattatag ttcctctttg attgtggtca ccggattaat actgacacaa ttggtttttc
    36061 ttttcggtgt ctcatggttt tgttcttatg cgccagaaaa aaaggactat tttttaagtg
    36121 tctacactgg tgcaggcgtg ccattaacgc aattcatcgt agtaacaagc atgctagttt
    36181 tcagtaaatt aaatctgaaa aaagctttat cttctaaata aaaagaccct tggtagctag
    36241 atttttaacc taactatcaa gggtctttgt gtggaaaaat cagtttttat tcgatttctt
    36301 ctaccgtttc ctgcaaaagc tgttcatatt ttttgtttcc tttatataaa tcaccaatcg
    36361 tccatgccaa caggagaagg acaagaacaa tgattacata agcgatgtga tcagccaatg
    36421 ccagttgagc ggaagtggct tgctcgccga agaaactttc gatctgtcct ggtaatcctc
    36481 gtaagttcaa gtaagttaga ctaatcacag agaaccagcc aagaagtttt acgatgaatg
    36541 aatttttaaa tcggctaccc atctccgctt ggctgttggt catcatcagt aaaggaatca
    36601 tcgaaaatgg caacgcaaaa gccaagaaca cctgggattc attcattaag ttattcaaag
    36661 cggtatgttc ttcgatcgtt ccttttgtac ttgtaaacaa aacacaaatc aatacaggaa
    36721 tcacagaaag caatcgtgtg accagtcttc ttagccaaat cgggatacgc atatggatga
    36781 atccttccat gatgacttga ccggtcaacg taccagtaat agtagagttc tggccagaag
    36841 caagaagtgc tacagcaaat aaagtggaca agataccaga ttttgctaca gcaatcaaaa
    36901 taccattgct catagctgat ggatcagata aagcttcata cagaccaaaa aaggaaggat
    36961 cttggacaga accggttttg aagacagcga cacccatgat caatagcagt gcattgacta
    37021 gaaaagccat tgacaactgg atattagaat cccaagtaga aaaacgtaca gcttgtgcta
    37081 catcttcttc gttttggtga tcgatcttcc gagtttgtga aactgctgaa tgaagatata
    37141 agttatgcgg catgactgtt gcaccaataa tccctaaagc tccagttagc ggagtttcgc
    37201 caccaatgac aggtgagtct gagaatgtat ggctgttagg gataaagcca gcaaataaac
    37261 tgtgccaatc tgggttagac aagccaactt gatagacaaa aacgaataaa atgacgaaaa
    37321 ttaagcagac aaccagtgct tcgatcttac gaaacccgac ttttgtcagt agtaagagaa
    37381 cgaaaacatc taatacagta ataaagacgg caatcaccaa tggaatatca aaaagtaagt
    37441 agagggcgat tgctgcacca attacttctg caatatctgt tgccataatg gctaattcag
    37501 tcaagatcca taaaatgaca cctaatgtct tacttgtgcg tgctcggatt gcctgtgcca
    37561 aatccatttg gctcacgatt cctaattttg ctgccatata ctgtaaaagc atagcaataa
    37621 gactagacat caaaataact gacatcaata aatattggaa gttttgtcct ccggtgatcg
    37681 aagtagacca gttacccgga tccatatacc caactgctac taacgcacca ggtccagagt
    37741 aagcaagaag tgttttaaaa aaacttttcc cctttggtac atcaactgtt ccatttactt
    37801 cagctaaaga taaaccattt gtatgttcaa taagtcgatg cttatgaaat tgtttattgg
    37861 attgattatt caagaaaaaa ccacctttcc tatttatcta cgattacatt ctacacctca
    37921 aaaagaaaaa attaaagaaa aagattcggt atgcctaatt tattttcgtg gcgtgtcttg
    37981 ctagatgacg aaagaaattt tgttctcctc taaaaaaacc gctatttttt gcatgaaact
    38041 aaagaatacg cctttctttt ttcaataaac tttagtaaaa taagtcattg agagcaattc
    38101 ggaaatagaa aggagagatt tatatgttat caacaggtat tgtcgtactt attgctatta
    38161 ttgctttgct aatcggcgca gtaggtggct ttttcctagc gcgtaaatat atgaaagact
    38221 atttagaaaa aaatcctccc gttaatgagg acatgttgcg atcaatgatg atgtcaatgg
    38281 ggcaaaaacc atctgagaag aaaattcgcc agatgatgca gcaaatgaag aaccaaggga
    38341 aaaaataggt tctgacccaa cgacttttta cgggctgatt ttttcagccc gttttttatt
    38401 tcgtaatttg ttagactatt cagattattc agcaaaaaag acaagcacag acaatcctag
    38461 gctcttgata aagacaaagg aggagtaaaa tgtcgatttt tagaaaatta ggctggtttt
    38521 ttcgtcagga aaagaaacat tatttaattg gagttttctc gctattcgca gtggccttgg
    38581 tacagttagt tcctccaaaa gtgattggga tcatcattga tgagattgcc gataaaaatt
    38641 tatcaataaa gatcattttc gggtggatct tcctattgat tatcgctgct tttttacaat
    38701 atctttttcg ttatatctgg cggacgaata tttggggaag tgcagctcgg ttggagaaag
    38761 aacttcggca gcagttattc aaacatttta cagaaatgga caatctcttt taccaaaaat
    38821 atcggactgg ggatttgatg gctcatgcaa cgaatgattt gaatgcgatc cagaatgtag
    38881 caggagctgg tatcttgacc tttgcagatt cagtgattac gggcggaaca acgatcattg
    38941 cgatgatttt gtttattgat tggcgcttaa cactgattgc gatgattcct ctgcctttgc
    39001 tggccatcac ctcacgagtg ctgggatcga aactccatga tgcttttcgt gattcgcaag
    39061 aagctttttc tagtatcaat gataagacac aagaaagtat cactgggatc aaagtcatta
    39121 aaacatttgg acaagaaaaa gaagatctag cggattttga agaaaagatt gaagatgcaa
    39181 ttattaaaaa caaacgagtc aattttttag actccttatt tgatccattc attaccttga
    39241 ttatcggtct gtcttatgtt gcaacaatta ttttaggtgg aaagtatgtc ttggaaggca
    39301 cgatctcgat cgggcagatg gtctcattta ttagttatat cggtatgctt gtttggccga
    39361 tgtttgcgat tggtcgattg ttcaacgtat tggaaagagg aaatgcttct tatgatcgcg
    39421 tcaatgaact tttgcatgaa aaaacgcata tcgtggaaca actgggtgca gtgcagaccc
    39481 cggcaaaagg cgagctgaat gtagctattt cctcttttaa ttatctgaaa gaagaatcac
    39541 cagcattaga acaaatcaag tttgcaatcc aagaaggaga aacattaggt attgttggga
    39601 aaactggtgc gggaaaaacg acgatcatca aattactgat gcgtgaatac gatgattatc
    39661 aagggaaaat ccaatttggc ggacgttcga tcaaagaata ttcattgaat gctttattag
    39721 gatcaatcgg ctatgtgcct caggatcact tcttgttttc gatgaccatt cgagataata
    39781 tccgtttttc taatccaaaa ctttcagaag aagaagtaaa gcatgcggct tctttagcat
    39841 ttatcaacaa tgaaatcaag gaattaccaa aaggttatga taccttagtt ggagaacgtg
    39901 gagtctcttt atctggcgga cagaaacaaa ggatttcgat tgctcgagca ttgatcaaaa
    39961 atcctgaatt attaatgtta gatgatgcac tttctgcagt agatgccaaa acagaagaag
    40021 ctatcttgca taatttaaaa gaaataagaa aagagaaaac aactatcatt gcagctcacc
//
LOCUS       DADXTG010000012        39198 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.012, whole genome shotgun sequence.
ACCESSION   DADXTG010000012 DADXTG010000000
VERSION     DADXTG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.012"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(281..2662)
                     /locus_tag="IWI57_000505"
     CDS             complement(281..2662)
                     /locus_tag="IWI57_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBC2448672.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2652..3299)
                     /gene="recU"
                     /locus_tag="IWI57_000506"
     CDS             complement(2652..3299)
                     /gene="recU"
                     /locus_tag="IWI57_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBC2448673.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3363..3902
                     /locus_tag="IWI57_000507"
     CDS             3363..3902
                     /locus_tag="IWI57_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBC2448674.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3992..4411
                     /gene="gpsB"
                     /locus_tag="IWI57_000508"
     CDS             3992..4411
                     /gene="gpsB"
                     /locus_tag="IWI57_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBC2448675.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4433..4800
                     /gene="rnpB"
                     /locus_tag="IWI57_000509"
     ncRNA           4433..4800
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWI57_000509"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5009..6175
                     /locus_tag="IWI57_000510"
     CDS             5009..6175
                     /locus_tag="IWI57_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBC2448676.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6221..7717
                     /locus_tag="IWI57_000511"
     CDS             6221..7717
                     /locus_tag="IWI57_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBC2448677.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7798..8481
                     /locus_tag="IWI57_000512"
     CDS             7798..8481
                     /locus_tag="IWI57_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2448678.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9126..10316
                     /locus_tag="IWI57_000513"
     CDS             9126..10316
                     /locus_tag="IWI57_000513"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBC2448679.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10418..11371
                     /locus_tag="IWI57_000514"
     CDS             10418..11371
                     /locus_tag="IWI57_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBC2448680.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11440..12789
                     /locus_tag="IWI57_000515"
     CDS             11440..12789
                     /locus_tag="IWI57_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2448681.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12972..13295)
                     /locus_tag="IWI57_000516"
     CDS             complement(12972..13295)
                     /locus_tag="IWI57_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448682.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13424..14587
                     /locus_tag="IWI57_000517"
     CDS             13424..14587
                     /locus_tag="IWI57_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBC2448683.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14657..16006
                     /locus_tag="IWI57_000518"
     CDS             14657..16006
                     /locus_tag="IWI57_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBC2448684.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16051..16482)
                     /locus_tag="IWI57_000519"
     CDS             complement(16051..16482)
                     /locus_tag="IWI57_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2448685.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16870..19512
                     /gene="alaS"
                     /locus_tag="IWI57_000520"
     CDS             16870..19512
                     /gene="alaS"
                     /locus_tag="IWI57_000520"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBC2448686.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19712..20308
                     /locus_tag="IWI57_000521"
     CDS             19712..20308
                     /locus_tag="IWI57_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2448687.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20430..21578
                     /locus_tag="IWI57_000522"
     CDS             20430..21578
                     /locus_tag="IWI57_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBC2448688.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21616..22029)
                     /locus_tag="IWI57_000523"
     CDS             complement(21616..22029)
                     /locus_tag="IWI57_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBC2448689.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22108..22539
                     /locus_tag="IWI57_000524"
     CDS             22108..22539
                     /locus_tag="IWI57_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBC2448690.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22638..22871)
                     /locus_tag="IWI57_000525"
     CDS             complement(22638..22871)
                     /locus_tag="IWI57_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBC2448691.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23054..24724
                     /locus_tag="IWI57_000526"
     CDS             23054..24724
                     /locus_tag="IWI57_000526"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBC2448692.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24871..25257
                     /locus_tag="IWI57_000527"
     CDS             24871..25257
                     /locus_tag="IWI57_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448693.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25244..25942
                     /locus_tag="IWI57_000528"
     CDS             25244..25942
                     /locus_tag="IWI57_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2448694.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25947..26411
                     /locus_tag="IWI57_000529"
     CDS             25947..26411
                     /locus_tag="IWI57_000529"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBC2448695.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26408..27319
                     /locus_tag="IWI57_000530"
     CDS             26408..27319
                     /locus_tag="IWI57_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBC2448696.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27554..28093
                     /gene="pyrR"
                     /locus_tag="IWI57_000531"
     CDS             27554..28093
                     /gene="pyrR"
                     /locus_tag="IWI57_000531"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBC2448697.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28107..29390
                     /locus_tag="IWI57_000532"
     CDS             28107..29390
                     /locus_tag="IWI57_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBC2448698.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29403..30329
                     /locus_tag="IWI57_000533"
     CDS             29403..30329
                     /locus_tag="IWI57_000533"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBC2448699.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30348..31628
                     /locus_tag="IWI57_000534"
     CDS             30348..31628
                     /locus_tag="IWI57_000534"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBC2448700.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31633..32709
                     /gene="carA"
                     /locus_tag="IWI57_000535"
     CDS             31633..32709
                     /gene="carA"
                     /locus_tag="IWI57_000535"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBC2448701.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32714..35899
                     /gene="carB"
                     /locus_tag="IWI57_000536"
     CDS             32714..35899
                     /gene="carB"
                     /locus_tag="IWI57_000536"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBC2448702.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35953..36729
                     /locus_tag="IWI57_000537"
     CDS             35953..36729
                     /locus_tag="IWI57_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBC2448703.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36726..37658
                     /locus_tag="IWI57_000538"
     CDS             36726..37658
                     /locus_tag="IWI57_000538"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBC2448704.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37655..38371
                     /gene="pyrF"
                     /locus_tag="IWI57_000539"
     CDS             37655..38371
                     /gene="pyrF"
                     /locus_tag="IWI57_000539"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBC2448705.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38378..39007
                     /locus_tag="IWI57_000540"
     CDS             38378..39007
                     /locus_tag="IWI57_000540"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBC2448706.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38995..>39198
                     /locus_tag="IWI57_000541"
     CDS             38995..>39198
                     /locus_tag="IWI57_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448707.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
BASE COUNT        12629 a         6923 c         8538 g        11108 t
ORIGIN      
        1 ttgataataa caatgggatt attaagaagt ggcactaaat aaaagactag tgtgtattta
       61 gccgatttaa ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg
      121 attttctaat ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa
      181 aaaatcatgt aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca
      241 caaaaaagaa accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg
      301 aggtgctgct ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac
      361 tggaagaagc aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag
      421 gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact
      481 cagcagttga ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg
      541 agcttccagg agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat
      601 tcgatctagc tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc
      661 ttggcatctc ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat
      721 aaacatcaga ggctacgtgt gacgattctg aagtgacagg tgtcattttt ttattgtaac
      781 ctgtccacac ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca
      841 ccccagaaga aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct
      901 taccggcttg atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat
      961 cttttaaaat gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg
     1021 gctcataagt ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt
     1081 aataagtccc tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc
     1141 cgtattttgt cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg
     1201 tactgtattc gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat
     1261 tgaataattt gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg
     1321 tgcccatata ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg
     1381 gtgcgtcagt aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat
     1441 ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc
     1501 ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat
     1561 tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat
     1621 ccgtgttgac gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct
     1681 ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt
     1741 tgacataatt atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat
     1801 ccgttaccgg tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa
     1861 gcatcgtata caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat
     1921 cataggcgga aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt
     1981 ctgataattt cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg
     2041 acatatacac tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca
     2101 atcttacagc catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg
     2161 tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc
     2221 cagaggtaaa gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt
     2281 tataaaaacg gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat
     2341 tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg
     2401 tatatagttt ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt
     2461 ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta
     2521 ttcctaaaag gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac
     2581 ctttgccaga attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc
     2641 ttctagtttg ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag
     2701 aagtcaaccg cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct
     2761 agttcttcga tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag
     2821 taagcaatca aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac
     2881 aagacaaagc agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca
     2941 tggaagtttt taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag
     3001 taaaagcctc gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc
     3061 ttgatcacag cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc
     3121 tttttatgga taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct
     3181 tcttcaaagc gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt
     3241 ttttcgacgg gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc
     3301 tttaatcaca ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg
     3361 agatggataa gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt
     3421 ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc
     3481 agttagcaga agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat
     3541 gggctgtgca aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt
     3601 tgtatccttt ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat
     3661 tagcagaaca attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta
     3721 gtcagctaaa gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct
     3781 atgatgaaga attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag
     3841 cagatcatcc ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat
     3901 aatcgatatt gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt
     3961 tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga
     4021 cattttacaa caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga
     4081 attcttagac aatatcatca aagattatga aacttacagc aaagaactcc ttgctttaca
     4141 agaagaaaac gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc
     4201 tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat
     4261 cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc
     4321 aagtgcagtc aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt
     4381 agatgataat gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg
     4441 gtaatcgcgg tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc
     4501 ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga
     4561 aaacaagcta aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg
     4621 gctggggaaa cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg
     4681 gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag
     4741 atggttaccg ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa
     4801 aaccgcaaac atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa
     4861 aacatttgaa agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt
     4921 ttgtatcacc ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct
     4981 ccttttttta taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc
     5041 aactgcggca agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga
     5101 atgccaagtt gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc
     5161 taatttatgg ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta
     5221 tacgtttgat gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact
     5281 agatgcagct tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga
     5341 ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc
     5401 agcttctgta ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa
     5461 agaccatgtg atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg
     5521 cttagaaaaa ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac
     5581 aaattggcga aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat
     5641 tgaagcagcc ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga
     5701 atcttgggat tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa
     5761 agcagattat gatattgaat tagatattac tggttcagat atcaatggac ggatgattga
     5821 aattgccaaa gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca
     5881 agcagtcaaa gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta
     5941 cggagaacgt ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt
     6001 attccgtcca ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga
     6061 attttacgga caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga
     6121 tctattccag tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta
     6181 ctaattgatt cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt
     6241 acaagaagta aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac
     6301 acaaacaggt atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta
     6361 cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc
     6421 tgaacatccg gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga
     6481 gtatgatttg gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc
     6541 ttcagcacac ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca
     6601 cgcattgaat caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga
     6661 aaagacgaat tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct
     6721 tgataaggta tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga
     6781 aaaagggact gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa
     6841 gagatttgta gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga
     6901 tgacactgtc cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg
     6961 atggaacgaa acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg
     7021 tatgtatgaa caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc
     7081 aatgggtatc catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag
     7141 cttctggaaa aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat
     7201 tccttttgac aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga
     7261 ggcagacagt ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat
     7321 tttcaatgaa ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga
     7381 gtatctggga atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc
     7441 aggtgcaagc tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt
     7501 acttcattct ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc
     7561 tccaatcaaa aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa
     7621 tcaattaatc atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat
     7681 gaaagacctg tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg
     7741 aaattcatct aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg
     7801 gataaattac aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga
     7861 catgggagcg atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa
     7921 ccgcaggcag accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat
     7981 aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg
     8041 ccaatagtag aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct
     8101 aaaaatctct ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat
     8161 cgtatcgaag cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat
     8221 gggcacccga tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac
     8281 cgaaaaggca cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa
     8341 atgaatactg attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc
     8401 ttagaagaat tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg
     8461 atcaaaaata acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc
     8521 ggatcatttt ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc
     8581 cgttcagctc cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata
     8641 ttgtaacttg tgattcggaa ttggcaggag aacaccgttt attcggaaga ataggactgt
     8701 gacaagactt ttgtcacagt ccttttttcg aattcaacaa gtaaaacttt taagaaatat
     8761 tcagagagta aaattaaaaa ttgctgataa acgaagatgg aaattttatg tatttttatt
     8821 ggtctatgca ataaataaat taaatattca gaatacaatt gattttaacg aaagggcttc
     8881 tatcaatggt tgtgttgtat ttatctgccc aggtagagta aaatagatca ttatctaaaa
     8941 ggtaattgtt catactatat aagtcatttg aaagtaaaaa gatactgtaa ataaaaaatg
     9001 tggttaaata attctcataa tcagtgtaag cgttatcaaa aatatagcat acttgctttg
     9061 tagatgaatg acacgatata tgtttggtat gttctgcaag acagatgaat atggaggtgg
     9121 agacaatgtc ttccaaagaa ctaacaatgt atttagagaa agaattggat caactgaaag
     9181 aaaaggggtt atacaatacg atcgacatac tagaaagtga aaatggtgct tgtatcatcg
     9241 tagacggaaa aaagatgatc aatctagcat ccaacaatta tcttggattt gccaatcgcg
     9301 aagaattgaa aaaagcatgt attgaagcaa ccgaaactta tggtgtagga gcgggagcag
     9361 tccgaacgat caatggttcg ctgaaaatcc atcagcaatt agaagaaaaa atcgcagaat
     9421 tcaaagggac agaagcagca atcgcctttc aatctggatt caactgcaat atgggcgcta
     9481 tctctgcagt aatgacaaaa gaagatgcta tcttatcgga tgaattaaac catgcatcta
     9541 ttattgacgg ttgccgctta tccggagcaa aaatcatccg aattaaacat caagatatga
     9601 aagaccttga gaaaaaagca aaggaagcga tagaaagtaa gaaatataaa aaaatcatgt
     9661 atatcacaga tggtgttttc tccatggatg gagacattgc acgattgccg gaaattattc
     9721 cgattgttga aaaatatgga ctgatcacct acgtcgatga tgcgcacggc tcaggcgtaa
     9781 ctggaaaagg tgcaggaacc gtcaagcatt ttggattaag cgacaaaatc gatatgcaaa
     9841 tgggcacgtt atcgaaagct ataggtgttg ttgggggtta tgtagctgga tcgaaaacac
     9901 taatcgactg gttgaaagct agaagtcggc cattcctatt ttctacttcg ctaactccag
     9961 gtgcagctgc agctgcattg gcttcgatta ctttgatgca agaacatccg gaacttgtcg
    10021 aaaaagtttg ggaaaatgct aattatttta aagaagaatt gaaaaaagta ggctataaca
    10081 tcggtatgtc tgaaacacct attactccag tcattttggg agacgaaaaa gtgactcaga
    10141 ctttctctaa gaagctgatc gaacatggga tttatgctaa accaatcgtt tatccaactg
    10201 tgcctcttgg gacaggcagg atcagaaata tgccgactgc ggaacatacg agggaaatgc
    10261 ttgatgaagc agtagcagtt tatcaaaaaa taggaaaaga aatggaaatt atttaactaa
    10321 atttagtttt ttcgtctatt taaaattaga gaacatgttc acgcatatat tgttaatcat
    10381 ctgaagatag tagtaaaaaa gatcagggag gatcatcatg aaaaaagtat tggtgacagg
    10441 atgtttagga caaattggtt cagagttgat cacacggcta cgtcaagaac taggcagtga
    10501 aaatgtcatt gcgacagata tacgcatgcc tgaagtcatt gagaaaaacg atatctttga
    10561 gatcctagat gtaatggatt acgaaaaaat gcgcagcata gcagaacaat atcaagtgga
    10621 cacattgatt catttggctg ctcttttgtc agctgttgca gaagcaaaac cacaattagc
    10681 ctggaatttg aacgtgactg ggctggtcaa tgcattggaa gtcgctagag aattccagct
    10741 gaaatttttt acgccaagtt caatcggcgc gtttggtcca aatactccaa aagatcatac
    10801 cccacaagat actcttcagc gtcctacaac catgtatggg gtaacgaagg tagctggaga
    10861 actactatgt gattattatt atgtgaaata tggcgtagat actcgtggtg tccgtttccc
    10921 tggattgatt tcttatgcaa ccttgccagg aggcggtacg acagactacg cagtcgatat
    10981 ttattattca gcaatcaaag aaggaaagta cactagtttc attgatcaag gtacatcaat
    11041 ggatatgatg tatatgccag atgcgatcga tgccattata aaactcatga atgcagatcc
    11101 tgatcgattg gttcatcgaa atgccttcaa tatatcggcg atgtcttttg acccggaaga
    11161 gatcaaagca tctattcaaa aaatcatacc agattttgaa atggattatg atgtggatcc
    11221 tgtacgtcag tcaatagcag aatcttggcc aaattcacta gatgtttctt gtgcgcaaga
    11281 ggaatggggc ttttcgccga aatacgactt agatgtgatg acaaaggata tgttggaaaa
    11341 actgaaagaa aaattggtag agacggtata aaacaagaac cacatccaaa aagtttttta
    11401 tacttttggg attggctatg gtatgaaagg ggacaaggga tggaagaata tcaagccacg
    11461 ccagtaaaag aagtaaaaat agaaaatgaa ttaaaacgta cgatgggatt ttttactgct
    11521 ttatcgactg ttatgggcac agtgatcggt gcaggggtat tttttaaagc cgcaagcgta
    11581 gcagaagtta caggatccgc cagtttgcat atgttttctt ggtttctcgg tggtatgatt
    11641 tctgtctgtg caggactaac aggagctgaa ctggcagctg ctattccaga aacaggaggg
    11701 atgattaaat atatcgaacg gatttacgga aatacagcag cttttttatt gggctgggcg
    11761 caagtcgtga tttattttcc agcaaatgta gcagccttgt cgatcatttt cggtacacag
    11821 ttcgtcaatc tatttggtct ttcccagtca atgattgttc ccgtagctgt tacagctgct
    11881 gtatcgatcc tgttgatcaa ttttttaggt tctaaagctg gaggggcttt tcagtcgatt
    11941 acgcttgtct gcaagcttat tcctttgttt gtgatcgtca ttttcggttt gttccgacaa
    12001 gaaggagtag acttccaact gtttccgatc caagcaggag aaaatctttc atttttctca
    12061 gccttgggag cgggcttgct agcaacgatg ttcgcatacg acggttggat acacgtagga
    12121 aatatttcgg gagaattgaa aaagccggcg aaagatttgc caaaagcaat ttctttagga
    12181 atcatcggaa tcatgatcgt ttatttatta gtaaatgcgg tgttcttaag aaccgcatct
    12241 atcgacggag tcgcaggaaa cagcaatgcg gcaagtgacg ttgcaaaaat gatttttggc
    12301 ggcttcggtg gaagactggt cacagttggt atcttgattt ctgtttatgg aaccatcaat
    12361 ggctataccc ttactggaat gcgtctccct tatgttatgg caaaagaaaa caacctgcct
    12421 tttagcaagc tctttgctaa acttcatgat aaaactaaag ttcctgtagc cgcaggtatc
    12481 ttagagctag tcatcgctat tggcatgatg atgatcggtg gttttgatac actaacggat
    12541 atgctgatct ttgtcatttg gattttttat acgatggtct ttgttggtgt cattcttctt
    12601 cgtaaaaaag aacctgattt gtttagacca tacaaagttc caatgtatcc gtttattcca
    12661 ctagtagcaa tcattggagg gacttttatc gtatcaagca cgcttatcac tcagaccttt
    12721 ttagcttcga tggggatagt attgacactt gctggtatcc cgatttatct ctatttaaaa
    12781 cgtagatagc taaaacattc gaattttatt agaactgcac ttctctgcca aacaaaatat
    12841 tttatttaaa tgaggagtac gtggaagaaa tgtcaaaagt actcgttaat tttgatcacg
    12901 taaatgtcgt tttgtctacg atgaaatagc aaagtaaaag acagaaatcg ggctcaaccg
    12961 atttctgtct tttactttga tttgcgtaac tgaactattc ggcgtacgcc gcgataaata
    13021 tttaataaaa ccaagcaacc agaaatgaaa atgggaagcc aaatcagata gacagggtag
    13081 ccaagcactt tagacaaagc atgtggagtc attttatcca tttcaagaag tgcttgctgc
    13141 caagtctgaa gattgaagac tagcatgaaa cccgaaataa tcaggagaat aaatcctaaa
    13201 taatgaattg tagttctttt tcttacataa gcgatcgtag cataaaggaa cagtaaaaaa
    13261 ataggtacgc caaaaacaaa gtacatattc agcatctcga cacccccttt gtgtttatct
    13321 tactttactt tgaaagaaat gagaaataat agcctcgtta cttgcttgaa acagcaaagg
    13381 actgctaaga taagagagat acaaagaatc aaggaggagt catatggcaa gttatatcga
    13441 accgattcgt tttgcgctga ttgtttttcc atttttagcc ttagcgatct catctgtttt
    13501 ttttatttat gaatatcgaa agtatggaac atttctagta acaagggcag ttattttgta
    13561 ttctttcgtt ttttatttat tgtgtgcgta ttttttggtg atcttaccac ttccggcaaa
    13621 ggagacggtt gcccaaatga cagggccaaa gatggagctg catttatttg cttcatggca
    13681 acatttcaga gacaacacgg tattggtctt gtctgaacct agtacctacc tgccggcaat
    13741 gaagcagagt gtatttttgg aaccagcttt taatgtttta ttactcatac catttggcat
    13801 ttatttaaga tattatttcc ggctttcttt tatgaaaacg atgattgcta gtttctgcct
    13861 ttctctattt tttgaattga cgcagctaag tggactttat tttatctacc caagaccata
    13921 ccgcttattt gatgtgaatg atttagtgac aaatacattg ggtggaatca ttggttttgt
    13981 cgttacgccg ttatttacat ttatgcttcc tacaagaaga cgaatggatg aactttctta
    14041 tgaaaaagga aagactgtct cattgactcg acgcttggtc gcttatttga tcgactggac
    14101 atttttatct cttattaatt tggtagcggc gattttcttt aaaattgcgt tcgattcagg
    14161 ggcagttact tctcatatct ggtggctttt attagaagta ggggtatact ttatgattgt
    14221 accgtttata accaatggat ggacacttgg caaaaaaatt gtgcgtatcc gtttggtgga
    14281 agaagaccgc gaacgaattt ctttaaaagc attgatgatc cgctacgggt atctgtactt
    14341 tcttttttat ggtttaagct ggttggggca gttgcttcag caaatcatga attcgacaac
    14401 gaatacggtg ttgcagatga gtgcagtatt catttctctc ggtgtcttgt tactacaagg
    14461 tatttttatt ttaaatatct tgctatcatt tttgagaaaa aaacggattc tgttttatga
    14521 aaaagctagt cacacatata ctgttagcac gattggccaa acaaaggatg atgattctcg
    14581 aaattagctg agcaaaaacg ctttgtctgt gttataatgc caaagtcgtg ataatttaaa
    14641 gagaagaggt gcacgaatga ataattttga tatattccgc ttcaaaccgt ttatcaatga
    14701 agcgttaacc gcaaagggct tcaaacagcc cacagaggtc caggaacgtt tgattccttt
    14761 aattgcaaaa ggaaaaaata tcattgggca atctcaaact ggtacaggaa aaacacatac
    14821 gtttttgttg cctttgtttg aaaaaatcaa tgcagaaaaa gaggaagtac aagttgttat
    14881 tacagctcca agtagggaac tagctgcaca gatctatgaa gcagctacac aaatcgctaa
    14941 attttctaca ccggagatcc gtgtctcgaa ttttgtcggc ggtacagaca aacagcgtca
    15001 attaaatcgt ctgcatcacc aacagccaca ggttgtagta ggcacgcctg gtagaatatt
    15061 agacatgatg aatgaacaag cgctgaagat tcacaccgca tttgcatttg ttgtcgatga
    15121 agcggatatg acattggata tgggtttttt aaatgaagta gaccaaattg caagtcgatt
    15181 accagaaaag ctgcagtttt tagttttttc tgccactatt ccagaaaaac ttcgtccatt
    15241 cttacgaaaa tacatggaaa atccaatcat tgaagaaatc aaaccaaaag cagttatttc
    15301 tgaagacatt gaaaattggc tgatttcaac aaaaggtaaa gatgagaatc aaatgattta
    15361 tcaactgctg acaattggtc atccttattt agcaatcgta tttgccaata caaaacagca
    15421 tgtggatgaa attgccgatt acttgaaaaa ccaaggcttg aaagtagcaa agattcatgg
    15481 agacatcacc ccaagagaga gaaaacgagt gatgcgacaa gtacagaatc tggagtatca
    15541 atatgttgta gcgactgatt tagctgctag agggatcgat atcgaaggtg tctctcatgt
    15601 tattaatgca gaaattccag aagatctaga cttctttatc catcgtgtcg gacgtactgg
    15661 acgtaatggc ttaaaaggaa cggccattac gttatattcg ccaaatgatg agcaagccat
    15721 tgatgcgata gaaaaattgg gcgtctcatt tgaaccaaaa gaaatcaaaa acggggaaat
    15781 cgtcaaaaca tacgatcgta atagacgtgc caaacgagaa aaatcaaaag atgtattaga
    15841 tcccacgctg attggtcttg tgaagaagaa aaagaaaaag atcaagcctg gttataagaa
    15901 aaaaatcgat tgggcaattg cagaaaaaaa taaaaaagaa cgcaaaatcg aacgcagaca
    15961 gcaaagccgt actgctcgta aaaataagaa aaattcacat aaataaaaaa caagaggttg
    16021 tgacaaaagg cgtgtcacaa tctcttgttt ttaagctaga ctttttttca ttccgataca
    16081 aagtacttca tcttcaataa aaggttctgt gcaagtatga tacccttgtt tttcataaaa
    16141 atctacagcc gtcatttcag ctactacata actagtctga caaccttctg aaaccgcccg
    16201 tttttcagca gcttcaagca actgaccgcc taaccctttt cctcggtatt cttttgctac
    16261 gcaaaatcgt tccggcttga agcaagtagg tgttttcttt tcataacgca aagtagctac
    16321 tggtcttttt tgatcaagaa atagataata ggtttgttgc gggatatctt ttccgtcgaa
    16381 ttctagttct gcaggaatcc cttgttctaa tacaaaaact tgatatctca aaaagcagct
    16441 agctgcgcgt atccattcat ccgctccaac tatttttttc atcatattcc tccttgtcat
    16501 gaatagcaga gatgtattga ttcctttatt cttatcgtac caaaaatcgt ggagtatgac
    16561 caaaaggaaa tgaaaataaa aattgagata ttgacagtga tcaatagaat gcgtatacta
    16621 attaagtacg aaaaggatat gatttgtttt ttaagactca cagaaaattc tttcttggct
    16681 gaaaaaagaa tagtcactaa tcaaattgct ccctttttct attttggtag aaataccgac
    16741 gcagctcagc gttatcgagt ttaagaggta atgattacac atcattgcaa tcaaggtggt
    16801 accgcgagtt ttcgtccttg gctgtgatgg tgtttttttg tatttaattt atagaaagca
    16861 ggtaatgaca tgaaacaatt aagtagtgca caagttcgac aaatgtttct tgatttcttc
    16921 caatctaaag gacatacgat tgaacctagt gcgtctttga tccccgtgaa cgatccaacc
    16981 ttattgtgga tcaactctgg tgttgcaaca ttgaaaaaat attttgatgg ttcagttgtg
    17041 ccagaaaatc caagaatcac aaatgcccaa aaatctattc gaacaaatga tatcgaaaat
    17101 gtcggtaaaa cagcacggca ccatacgatg tttgagatgc tgggtaattt ttcgattggt
    17161 gactatttca aaaatgaagc catccactgg gcatgggaat ttttgactga tccaaaatgg
    17221 atggcattcg atccagaaaa actttatgta acagtttatc caaaagatac agaagcaaaa
    17281 cgcatctggc atgaagaagt tggcctgcca ctagatcaca ttattgatgt agaagacaac
    17341 ttctgggata tcggtgcagg cccatgtggt ccggatacgg aaattttcta tgaccgtgga
    17401 gaagcttata acgatttagc agaagacgat cctgaaaact atccaggagg agaaaatgaa
    17461 cgttatttag aaatctggaa cttagtattt tcagaattca accataagcc tgataacaca
    17521 tttgaaccac ttcctcataa aaacattgat acggggatgg gattggaacg catggtctca
    17581 atcgttcaag atgcgtcaac gaactttgag acagacttgt ttatgcctat tatccacgcc
    17641 gtagaaaagc taagcaaaca agtaaaatat ggtgaaaacc cagtaacaga tatttcttac
    17701 aaagtaatcg cagatcatat tcgcgccctt tcatttgcta ttggcgacgg tgcattgcca
    17761 tcgaatgaag ggagagggta tgtcttacgc cgtttgcttc gtcgtgctgt catgcatggg
    17821 aaaaaattag gtattgaaca agcattctta tatcgtttag tccctgttgt tggggaaatc
    17881 atggtaagtt attatcctga agtacttcaa caaaaagaat ttattgaaaa agttatccgt
    17941 acagaagaag aacgtttcaa tgaaacaatc aatgaaggtt tggaaatctt aaatcaagtg
    18001 atccaagaag taaaaaacga aaacgggacg actctagcag gaaaagacat cttcaaacta
    18061 tacgacacat atggcttccc ggtagaatta acggaagagg tggcagaaga tgaaggactg
    18121 aaagttgatc atgctggttt tgaaaaagaa atggatgcac aacgcgaacg tgctcgctct
    18181 gcccgcagta ctgaaaaatc aatgggggta caatctgctt tattgacaga tatcaaagta
    18241 gaaagtaaat tcattggata cacagaacaa gaatcagata gtcaattatt ggtcatcgtt
    18301 aaaggtgagt ctcttttgaa tgaagtcgca gaaggtgaag ctcaaatgat ctttgatcaa
    18361 acacctttct atgcagaaat gggtgggcaa gtagcggatc aaggatggat catgaatgaa
    18421 gtaggcgaaa tcgtagcagt tgttacagat gttcaaaaag cgcctaatgg tcaatttatg
    18481 cacaccgtag aagtaaaagc accaatgagt gaaggacaaa cctatcatct attgatcgat
    18541 caatcgatgc gcaatcggat catcaaaaac catacagcaa cccatctact gcacaaggca
    18601 ttgaaagaag tacttggcga gcatgcaaat caagctggtt ctttagtcgc tcctggacat
    18661 ttacgattcg actttaccca ctttggtcaa atcacttcag atgaattagc tgaaatggaa
    18721 agaatcgtca acgaaaaaat ctgggaagct atcccagttg aaacaattga aacagatatc
    18781 gatacagcca aaaacatggg tgcaatggct ttgtttggtg aaaaatacgg acatgacgtt
    18841 cgagtagtaa atattggcgg ctggtcaatc gaactgtgcg gaggcacaca tgtagcaaat
    18901 acagaaaaca tcggaatctt caaaatcgtt tctgaatcag gaattggtgc aggcgtgcga
    18961 cgaatcgagg ctgtaacaag taaagaagcg tacgaactac ttgaagaaga agaaaaacaa
    19021 ctaaaacaag tagcaacaat tgttaaatca cctcaattga aagaagttgt ttcaaaagcg
    19081 gaacaattgc aacagcaagt aagagagcta caaaaggaaa atgaacaatt agctagcaag
    19141 ttagcgaatc aacaagctgg tgatgtattc aaagatccag aaacgatcaa tggaaaaaca
    19201 atcattgcag cacaagtaaa agtgaaagat atgaatcagc tgcgtcaatt agctgaccaa
    19261 tggaaacaaa aaaatatttc agatattctt gttttagcaa acgaacaaga aggaaaagtc
    19321 agtctgctag cggcaatgac gaaagaatca aatgaagctg gactaaaagc tggcgacttg
    19381 attaaagcaa tcgcaccgaa agtaggcggt ggcggtggtg gacgtccaga catggcacaa
    19441 gctggcggga aaaatcctgc tggtattcca gatgcactta aagctgcgaa agaatgggtt
    19501 gcgcaacaat agggcgttta cgtttgacag aaaaacaaaa gttcaaacat tgaacgaaac
    19561 atgattatgg gggcgaagac aaacaagtct tgccctcata cttttttcaa ttggatggcg
    19621 catctaatca aatggtcgga ttcggatggt ctttagttga cgaaagtgac agaaattttt
    19681 acgattagta taaataaaaa ggggtgaaaa aatgaatagt agaaataaga cctttcgact
    19741 agtcttacgt gcgatcttgt tagcaatcat catcgtacaa gcgatggttc cttggctggg
    19801 gtttattcca ttaggattca ttagtttaac aattatccat atcacagtaa ttattgctgc
    19861 agtagtatta ggcccaaaag atggcatggt gattggcttg ttttggggaa tcgctacaat
    19921 tgttcgtgcc tatgccatgc caacaacgcc atttgataca ttagtgttca cgaatccgat
    19981 tatctcggta gttcccagag tacttgttgg cttagtagca ggcttggttt tccactggat
    20041 ttatcaaaaa aataaatcaa taacgatcag ctcagccttt gcaggggtac tgggttcatt
    20101 ggtcaataca gtcttagtgt taggattcat gggattattt tatacaggtg caacggcaga
    20161 tgcctatggt gtagactctg ctcttttatt taaaacctta gcaggggttg cagcaatcaa
    20221 tgggatacca gaagcaatag gtgcgggaat cataacacca ttactagcaa aagcgttatt
    20281 tgcagcaaca ccgttgaaac cagaataatg accatctttt aatagtaata ggacgcacga
    20341 tagatagaaa aaggaagaga ggagaggaaa acaatcgtcc tctccatctt tttacctgag
    20401 caacaagcaa actagaaggg gtaaaaaact tggctaagaa acaagaagaa aaaaagaaac
    20461 aaataccgca acagcggaca aaaaaaggag tactagcggg tatcttagga ttggttacga
    20521 atctgatctt gtttgtctct aagctaatga tcgggctttt ttcaggaagt gtctccatta
    20581 tggctgatgc agtgaacagt ctatcagata ctgcttcatc catattgact ttgatcggat
    20641 ttaaaattgc tgcaaagcca gcagatcagg aacatcctta tggacacgaa cgcttcgaat
    20701 atatcagtgg gttatttgtt tcgatcatca ttacttatgt aggatttcaa tttttagagt
    20761 cttctgctga aaaaatcatc catccagaaa atgtctcttt aacacccatc gtcttttttg
    20821 tcctaatcat ttccatcttt ttgaaatttg cacaaggcaa aatgtatcag gttattgcgc
    20881 aatccattga ttctgaaaca ttgagagcta cagcaacaga tagttataat gatgtcttta
    20941 ctacactggc tgtattgctt tcagcagcaa tcgaatgggt aactggatgg cgtattgatg
    21001 gatatatagg ttttgcttta gcattatata tcctttatag tggattgacg atgcttcgaa
    21061 attttgtata cgaactatta ggaagtcgcc caacgccagc agagattgaa gaaatggaag
    21121 agcatttaaa tgcttgcgat gatatacttg gctatcatga tttattggtg cataactatg
    21181 gacccaataa acgctttgca tcagtccaca tcgaagtaga tgaatcaatg aatttgaatg
    21241 tggctcataa gattattgat ctgatagaaa aagactttaa aaagtctttg gatgtggaac
    21301 ttgtctgtca cttggatcca atgcctgttg ataacgaaaa atactataat atcttgcggc
    21361 agttaaagca aattcttctt gaaacaggta cagggcttaa tattcatgat tttagagtaa
    21421 ttcatcaagg aaaaacgctg caatttgatg tagtggtccc tgagaaattc cagtttgttg
    21481 atacagaact agaaagaatc ttacgtagtc aaatagaaga aaaaatcgga gattaccaat
    21541 tggaaattac gtttgatcac aattatcttt tatattaaat gagaaactat aaaaagactc
    21601 cttaaagttt ttaatttaaa gagtcttttt ttcttttaat tgtttatgga gagcctgacg
    21661 tgcaagatta tctgctcctt tatttttgtt ttcgggcaac cattgcagga tcaataaaga
    21721 aaattcggat agaagttctt ggattctttg aaggtaggga aggaagagct cgttcttcgt
    21781 tgcgtttttg tcgaccgtag aaactagtgt tttgctgtct gaaaaacaga aaatcgtttc
    21841 attctgtaag tcgtttttct ttagaaattc taaagttttt aaaaagactt cgaactctgc
    21901 ttgatggttt gtccctatag aaagcgggat agatagctgt tcttgtttac cttgatagac
    21961 aatcaagatt ccacctccac taggaccagg gttgcctttt gtagaggcat caatgtatac
    22021 ttttaacata aaaagagatt ctcctttttg ttcaaactaa tgttatggta agattatatc
    22081 attactgaat cggtgagagg ttagagaatg aaaaaaaata ttcgatggca gcctgaactg
    22141 gcacatacga tcatctattg gtcattgacc ttgatcattc tgttttttag tctgattctt
    22201 tctttagaaa acacaggtcc ttattggaaa agcaattttg ttttaggtat ttttcttgtt
    22261 tttctatttc taggtttgcg gcgttctttt attttaaaat caaactctgt gattatacgt
    22321 tatgctgctt tttggaaaga ccgagaaata tctgtcggac agattcgatc tttgacgcca
    22381 actaatactg gggtgcaaat gatgttgcaa gatgagaaag ccccatctgt tttcatgatg
    22441 cgaaaaaaag atcaagtagt cctttttgat cattttgaaa aaaacggacc caaatcagaa
    22501 tcaattcctt atccttatac gaaaaaaaga aaaagctaat tttaaaaatc agaacctaaa
    22561 aaaacgaatt tttacaggaa aagaaaagag tgagacgtac ggttcatccc gtttagcgtc
    22621 tcactctatt tgtttatcta ttcatgcata ctagtatcgg tagtctgaac gttgcctttc
    22681 ttgacatggg cagcttgtgt tcctcgattc ccttcgatga tctcaaattc gaccacttga
    22741 ttttcttcca aagttttgaa tccctcttct tcaatcgcag taaaatgtac aaaaatttct
    22801 tctgtgtcat cataactgat aaagccgtat ccttttttgt tgtcaaacca ttttacaatt
    22861 cctgtggtca aagcactcac gacctttcta aagtctgatc atagccttca ttataaagag
    22921 tatttataag tacgtcaaat aaattaaaaa cctgtaattt tatgtttttt ttctgaataa
    22981 aaaaaggtat aattggcata tcttaacaaa gaagatattt tgttgaataa agttcgtgaa
    23041 aaggggtaat caaatgaaga atgatctgca aatttcacaa gaaacagagc tagaaccgat
    23101 tgttgaagtg gctagtaaat tagatcttaa ggaagacgat ttggaactgt atgggaaata
    23161 taaggcgaaa atcaattttt ctggaatcaa tcgatcaaag gataacgctg aggggcattt
    23221 gatcttagtt acttccatca atccaacacc agctggcgaa ggaaagtcaa cgatcactgt
    23281 agggttagga gacgcgctca ataaaatcaa taaaaaatct gttattgcat tgcgagaacc
    23341 ctcacttggt cctgtgatgg gaatcaaagg tggtgctgca ggcggaggat atgcacaggt
    23401 attgcctatg gaagatatca acttgcattt tactggtgac atgcatgcta tcacaacagc
    23461 taataatgca ttatctgctc ttttagataa tcatattcat caaggaaatg aattgaacat
    23521 cgatgcccgc cgagtgattt ggaaacgggt agtcgatctc aatgatcgtg aactgcgtaa
    23581 agtaattgtt ggcttgggtg ggccaatcca aggtgttcca agagaagatg ggtttgatat
    23641 cacagttgcc agcgaaatca tggctatctt gtgtcttgcg gcagaccttg aagatttaaa
    23701 ggctcgtctt gcaaggatcg ttgtcggcta tacatttgac cgtcagcctg tgactgctgg
    23761 agacttgaaa gcagaaggcg ctctggcatt actgctgaaa gatgcaatca agccgaatct
    23821 tgtacaaaca atttacggaa caccagcttt tgtccacggg ggaccgtttg ccaatatcgc
    23881 tcatggctgc aatagtattt tagctaccaa gacagcattg agattagcag attacgttgt
    23941 cacagaggca ggatttggtg cagatcttgg cggagagaaa tttttagata tcaaagtacc
    24001 taacttgaaa aaagcgccgg atgctgtggt gattgtcgcc actgtccgtg cattgaaaat
    24061 gcatggcgga atgaaaaaag atgaacttaa aaatgagaat cttgatgctt tgaaagtagg
    24121 ttttgcaaat ttaaagcgcc atatacgtaa tatggaacaa tatcagctac ctgtgatcgt
    24181 cgcaatcaat gaattcgtaa cagatacaga caatgaactc acactgttgg agcacttgtg
    24241 tgaaaatcag gggattcttg ccaagagagc gagtgtatgg gcagatggag cagaaggcgg
    24301 cgttgattta gcaaaagctg tagtacgatt gattgataga aaagaagcta attacaagcc
    24361 attataccgc ctagaagaaa cgatacaaga aaagacagaa acaatcgtca agaaaattta
    24421 cggaggaaat ggtgttgttt tcagcaaaaa agcgcaaaaa caaatagaag agtttacgaa
    24481 gaatggatgg gacaaactgc cgatttgtat ggcaaaaaca cagtattcat tttctgatga
    24541 tccttcactg ctaggtgcac cggaagattt caccatcacg atccgcgaaa tcatcccaaa
    24601 attaggggca ggatttttag tagccttgac tggagatgtt atgacgatgc ctggtttgcc
    24661 taaaaaacct gctgcattga atatggacgt gacaaatgac ggagaagtgt tgggattgtt
    24721 ttaagcttga ttataatcta ccatcgaagg agctgtattt tgactacagc ttcttttttt
    24781 agacgatagg gaattaattt tgtttacact aggatcattc tttttcttta ttgtattttg
    24841 agctatacta aagcataata ggagggtatg atgaaaacga catttataag aaaaaccggt
    24901 ttttacggta tgggtagctc gattcagcta agaatcaaca atgaaaaacg cagtgttctc
    24961 agtcatgaac aaaagataac gcttgatctg gaagtgccct ttacgatgca ggtcaccttt
    25021 tattggctaa aaagccccgt ttatacagtg aaagaagcaa agcctgctta tgtgatcaca
    25081 atgaattact tgattcttca aatctatccg tttttatttc tagcaactgg gttgggggcc
    25141 gttttcctac aaaatttatt tttcagtttt tttgcagtta ttgtgttgat tgcgttcttt
    25201 ttctatataa aaaaccgggc atatttgatc aaggaggaaa attatggcaa actctgatct
    25261 ttttttaatc agtttcaatc gaatcgaaaa atggatgaga gacgagatgg gaaatgcacg
    25321 aaatatggga tttaccgaac ttgtcagacg cctagctcaa agaaaacaat taatgatcag
    25381 aaaatacgaa gatgatcttt tacagcttgc tcagttaagg aatgcgatcg tccatgatcg
    25441 gatcgcagtc gattttgtca ttgcagagcc aaatgattgg acagtcaagc gaatccagct
    25501 gatcgaacaa gaactgattc agcctgaaac cgtgctgcct cgatttgcaa aacgtgtaac
    25561 aggttttgaa caagatcttc cgttacttga actgttaaag atagtagcag aaaaaaggta
    25621 ttcacaattt ccgttataca ataaaggaaa atttgtagca ttgattactt tacgtaatat
    25681 cgggttttgg ctagcaaaag aaagccaaaa aggtccggtt gatttaacaa ataaacgagc
    25741 gttggatttg atcatccaaa acggaaagta cacaaattat cattttgttc cagctaaaac
    25801 acatatttac gaggttgaag cgatgtttcg tgagcaaggg actttggaag ccgtacttat
    25861 cactaaagat gggaatcccg atggtaatct gctcgggatc gtacgacctc gtgatattta
    25921 taaacaaata gaaaaggatt gattttttgt taattattta ttggatattc agcgctttgc
    25981 tagtaggagt ggatcagttg gtcaaatggt ggatcactga taatctagcg cttggagaga
    26041 cgaaaaatct tataccaggt attttgtcac tgaatcatat tcgaaacact ggagccgcat
    26101 ggagtatgct agaagggaag atgtggttct ttactatagt gacactcatt gcagtcgttg
    26161 tgattctcac attgatgatt aaaaatcgtg aaaaaggtaa tcgctggttc atgattggat
    26221 tgagtttgat tctagctggt gcgattggca attttatcga ccgtattcgt cttggctatg
    26281 tagtagatat gttccagaca gattttatga gctttccgat ttttaacgta gcagatgtga
    26341 cacttgtatg tggagtgatt tgtatgttaa tttatattat tttagatgaa aaggatcaaa
    26401 gaaaaaaatg acagaactta aaacgacgat tcaccatgaa tctggtagga tcgataaagt
    26461 attgactaat ttattcaccg attacagtcg ttcgcaaatt cagctgtggc tgaaaaatgg
    26521 agcggtttct gtcaatggac agccagtcaa agcaaattac aaagtaaaac aaggagacga
    26581 gattactgtc ttgatccccg aaccagaaac attagatgtg gtcccagaaa atatcccgtt
    26641 gacgatcgtt tacgaagatg aagatgtagc ggtgataaac aaacctcaag gaatggtcgt
    26701 tcatccttca gcaggtcata cgcatggcac aatggtcaat gcgctgatgt accatatcaa
    26761 ggatctttct acgatcaacg gcgttattcg tccaggtatt gttcaccgaa tcgacaaaga
    26821 tacttccgga ctgctaatgg ttgccaaaaa tgaccaggca cacgaatcat tagctcagca
    26881 attaaaagat aagacctctt tgcgcaaata tgttgcttta gtccatggag agattcctca
    26941 cgaaaaagga accatcaatg cgccaatcgg ccgttcgaag gaaaaccgga aaatgcaggc
    27001 gataagagaa gatgggaagt cggctgtcac acattttact gtattggaac gtttcgaagg
    27061 atttactctg gttgaattaa aactagaaac cggaagaaca caccagatcc gtgtgcatat
    27121 gaagtatatc ggttatccgt tagctggaga tccagtatac ggaccgaaga aaactttgaa
    27181 aggcaatggt cagtttctcc atgcgcaaat gttaggtttt acgcacccga ctacaaacga
    27241 aaagatgatc tttgaagcac ctcttccaga agtttttgaa aaaactttag aaaaattaag
    27301 aaaaaacatt gcattttaat ttagataacg gtatagtaat taacaacgaa agcataaaac
    27361 ttaatagaat tcctttaagt ttagtcccgt gaggctaaga agggtgggag caaggatacg
    27421 tatggtgcgc cataagtaga ttgctcattg acatagtata gctatcagtg tatcagtata
    27481 cgctcagtct aaacattcct cctgtcctct tagggcagga gtttttttat gcaaaaaagt
    27541 agggggaatg aaaatgcaag caaaagaagt agtagatcag gtaaccatga agcgagcatt
    27601 gactcgaatt acttatgaga ttatcgagag aaaccatagt atccaagata tcgtattggt
    27661 aggtatcaaa acacgaggaa tctacattgc atcaagaatt gccgaacgcc tgaagcagct
    27721 agaagatatc gatattcctg taggagaact ggatatcact ttataccgag atgacaaaaa
    27781 agaaaatcca gaagaaccgg aacttcattc ttctgacatc ccagtttcct tagaaggaaa
    27841 agaagttatt ttgattgatg atgttttgta tacaggacga acgattcgag cagcaatgga
    27901 cgcagtaatg gattttggca gaccaagaaa aatttcatta gcagtcctgg tcgatcgtgg
    27961 gcatcgagaa ttgccaatcc gtgcagatta tgttgggaag aacattccaa catctcgggc
    28021 ggaagagatt ttagtagaga tgcaagagct tgatggacaa gatcgcatca tgattttgaa
    28081 agaggaagat tagaggagag agaaacatgt cggagaaaaa aggatttcat aatgatgacg
    28141 tggtgttaga tatccacgat cgcccaaccc caggacattg ggtaggatta agtttacaac
    28201 acttgttcac gatgttcgga gcaaccgtgt tagtccccat cttagtaggt atcgatccag
    28261 gtattgcgct agtcagttca ggactaggta cattggtgta cttatttact actaaaggaa
    28321 agatacctgc gtacttagga agcagcttcg ctttcattgc agcgatgcag cttttgatga
    28381 aaacagatgg ttatccagcg attgcacaag gagcagtcac aactggatta gtctatttga
    28441 ttgttgcgct gatcatcaaa aagatcggtt cagcatggct ggataaaatc ttgcctccaa
    28501 tcgtcgtcgg tccagtcatc atggttatcg ggttgggact tgcggcaaat gcagcaacaa
    28561 atgctatgta taatcagggc acgtatgatt tcaagtatat tgctgtagcg ctgatcacat
    28621 taggattaac aattttcttc aacatgtggc taagaggttt tctcggattg attccgattt
    28681 tactaggtat tattagcgga tacatcgtag ccttgctgtt tggaatcgtg gatactaagc
    28741 caatcgtaga cgctgcctgg tttgctgttc ctaactttga gattcccttt gtacaatatg
    28801 aaccaaagct atatcttgga gcaatcacaa caatggcacc catcgctttt gtgacgatga
    28861 cagagcatat cggccatctt atggtattga ataaactaac taaacgtaat ttctttgaag
    28921 aaccgggtct tcataagacg ttgacaggtg atggattagc acaaatcgtg gcagcctttg
    28981 tcggaggtcc tcctgtaaca agttacggtg aaaacattgg tgttctggct atcacacgtg
    29041 tccatagcgt gtttgtgatc ggtggagctg cagtatttgc agtcatttta ggattcgtag
    29101 gaaaattaag cgctttgatt ttaagtatcc ctggaccagt catctcagga atcagtttta
    29161 tcttatttgg agtaattgcc gcaagtggaa tgaagatttt aatcgagaac aaaatcgatt
    29221 ttgataaaaa gaaaaatctg ttgattgctt cagttatctt agtagtagga attggcggac
    29281 tcgtctttga agtaggcaca tttacattgt cggcaatggc attagcaact gtacttggca
    29341 tcattttaaa tcttatctta ccagaaactt cacgaagcga agaaaactaa ggaggaaaca
    29401 ttatgatcat caaatcagaa cgtatcagct taaagcattt attgaccgtt gaggcactta
    29461 ctgatcaaga agttatgggc ttgatccgca gagggcagga attcaagcaa ggtgctaatt
    29521 ggactccgca aaagcgccag tactttgcta caaacctctt tttcgaaaat agtactcgca
    29581 cacataaaag cttcgatgta gcggagaaaa aactagggct agaagtgatt gaatttgaag
    29641 caagcacaag ctctgtccaa aaaggggaaa cactatacga tacagtactg acgatgtcag
    29701 cattaggcgt agacgtagcg gttatccgtc atggtgatga gaactattat gacgaactga
    29761 tacaaagtaa aacaatccag tgttcgatta tcaatggagg agacggaagc ggacagcatc
    29821 caacccaatg cttgcttgat ttgatgacca tttatgaaga atttggtcat tttgaaggtc
    29881 tgaaagttgc catcgtaggt gatatcactc actctcgcgt agctaaatcc aatatgcaga
    29941 tgttaaaacg tctaggcgca caagtattct tctcaggtcc tagagaatgg tacgacgatg
    30001 aattcgaagt gtacggacat tatatgccat tggatgaact actagatcaa gttgatgtca
    30061 tgatgatgct tcgggtgcaa cacgaacgcc atgatggaaa agaaagtttc tcaaaagaag
    30121 gctaccatca ggaacatggg ttaacagttg agcgtgcgaa gaagttgcaa aaacatgcaa
    30181 tcattatgca cccagcacca gttaaccgtg atgtagaatt agctgattca ttagtggaag
    30241 gattacagtc gcggatcgtt cagcaaatga gcaatggagt attcgtcaga atggcaatct
    30301 tagaagcagt gctaaatgga aaagcgtaat tataggagga tgaaaaaatg aaaacactca
    30361 ttaaaaacgg tcagatcaat acacgaaaaa atatgacgac accagcagaa atttggatag
    30421 aagatgggcg aatcaaagca atcggtacag gattctcaga agccgaattt gatgaagtgt
    30481 ttgatgcaaa gggacaattg atcacacctg gtttagtgga cgtccatgtt catttgagag
    30541 agccaggatt tacatacaag gagacgatcg aagctgggac tcgctcagct gctcgtggcg
    30601 gtttcactac tgtatgcgcg atgccgaatt taaatcctgt acctgatacg gcagaaaagc
    30661 tgagacaagt atatgacatc attagaaaag atgcagttgt gaaggtattg cagtatgcgc
    30721 caatcacaga aaatctgcga agtgagaaat tagttgatca agaagcactg attgaagaag
    30781 gtgcttttgc tttcacaaat gacggtgtag gtgtccaaac agctgggacg atgtatctgg
    30841 ctatgaaaga agctgcaaaa aacaataaag ccctagtagc ccatacagaa gatgaatctt
    30901 tattatttgg cggcgttatg cacgctggca agaaggcaga agaacttggt cttcccggga
    30961 tcttaagcgt aaccgagtct tctcaaattg ctagagatct gttgctagct gaagcaactg
    31021 gtgttcatta ccatgtttgc catgtttcta ctaaagaaag tgtccgtgta atcagagacg
    31081 caaaaaaagc aggaatccac gtaacagcag aagtttcacc ccatcatctt attttgatcg
    31141 acgaagatat tcccgaagat ttcggctttt ggaaaatgaa cccgccgcta agaggacggg
    31201 aggacagaga agctttgatc gaaggcttgc ttgacggtac gatcgattgc atcgctacag
    31261 accatgcccc ccatggatta gaagaaaaaa gccaaagttt catgaaatca ccatttggta
    31321 tcgtaggaag cgaaacagcg tttcaactga tctatactca ttttgtagaa actggtcgat
    31381 tcacgttgga acaagtgatc aattggttgg cagttaaacc ggctgagatt tttggtttga
    31441 atgcaggaac gctaacggtt ggtgcccccg ctgatgtagc agtttttgat attacacaaa
    31501 cttgcacgat cgataaagaa gactttttat caaaagggga gaatacacct tttatcggct
    31561 ggaaagtaaa aggcgaaaca caaatgactt ttgtcaacgg aaaacttgtg tggcagaagg
    31621 gagaataaaa gtatggaacg tttgcttata ttagaagatg gaacagtttt cgaaggaaag
    31681 gcttttggtg cagatgctag tgtcttcgga gaaatcgttt ttacaacaag tatgactggt
    31741 tatcaagaaa ctatcactga tcaaagtttt aacggtcaaa tcattacatt tacctatcct
    31801 atggtgggta attatggggt aaatagagat gattatgaat cgatttctcc aacatgcaaa
    31861 ggagtagtag taaaagaaca tgcacgtgta gcttcaaact ggcgaaacca aatgacgcta
    31921 gattcatttt tgaaacaaaa aggaattcct ggaatatcag gtatcgatac gcgcgccctt
    31981 actcgcaaaa tccgtgaagt cggaacgatg aaagctagta tagttgatgc tggagattcc
    32041 ttcgaacatg catttgatca gctgaaagca accgttctag caaccaatca agtgcagcaa
    32101 gtctcaacta acaaacctta tccaagtcca ggaacaggtc ggaaagtcat tgtcattgac
    32161 ttcggcttga aacatagtat tttacgagaa ttgagtaaaa gaaactgtaa cctcacagta
    32221 cttccctata acacagatgc cgagacgatt ttagcgctat caccagacgg agtcatgctg
    32281 acgaatggac ctggtgatcc aaaagatgtg ccagaagcaa tcgaaatgat caaaaaaatc
    32341 caaggaaaag taccgatttt tggtatttgt ctcggtcacc agttatttgc tttagccaac
    32401 ggtgcagata cctataaaat gaaattcgga catcgaggat taaatcaccc ggtacgagaa
    32461 gtagcgaccg gaagaattga ttttacttct caaaatcatg gctatgcagt cgatgaagcc
    32521 acgattgatc cagaaaaact catcgtaact catgtagaag taaatgacgg aacagtagaa
    32581 ggagtcaaac acagagatta tcccgctttc agtgtgcagt ttcatccaga tgctgcccct
    32641 ggtccgcatg atgcactaca tttatttgat gaatttatgg aaatgatgga cgcaggaaag
    32701 gagcagtaaa cagatgccga aacgtacaga tatcaagaaa attatggtga ttggatctgg
    32761 tccgatcatc attggtcaag ccgcagaatt tgactacgca ggaacacaag cctgccttgc
    32821 cttaaaagaa gaaggatatg aagtggtttt agtaaattct aatcctgcga cgatcatgac
    32881 ggataaagaa gtggcagatc aagtatatat cgaaccgatc acacttgaat ttgtatcaag
    32941 gattcttcga aaagagcgtc cagatgctct ccttccaact ttaggcggac agacaggcct
    33001 caatatggca atggagctgg cagcatcagg tattttagaa gaattgaaca tagaacttct
    33061 aggaacaaag ctttctgcga tcgatcaagc agaagatcgt gatctattca aacagttgat
    33121 gaaagaattg aatcagccaa tccccgagtc tgaaatcgtc aatacagtag aagaagctgt
    33181 agattttgca aataaaatcg gctatcctgt catcgttcgt ccagccttta ctttaggcgg
    33241 aactggtgga ggtatgtgtg aaaatgaaga agaattacgt atcgtatcag aaaatggatt
    33301 aagtctttcc cccgctaccc aatgtttgat tgaacgaagt attgcaggtt ttaaagaaat
    33361 tgaatatgaa gtgatgagag attcggctga taatgcaatc gttgtctgca atatggaaaa
    33421 ttttgacccg gtgggtatcc atacaggtga ttctatcgta tttgccccta gtcaaacatt
    33481 gtctgaccat gaatatcaaa tgcttcgtga tgcttctctt tcgattattc gagcacttaa
    33541 gatcgaggga ggctgtaatg tccaactcgc tcttgatcca catagtttta attattatgt
    33601 gattgaagtc aatccgcgtg tttctcgttc atctgcgtta gcaagtaagg cgactggcta
    33661 tccgatcgct aagctagctg ctaaaatagc gcttggatta acattagacg aaatgaaaaa
    33721 tcctgttaca ggaacgactt ttgcagaatt tgaacctgca ttagattatg ttgtagctaa
    33781 aattcctcgc tggccgtttg ataagtttga aaacggagaa cggcttttag gcacgcagat
    33841 gaaagcaact ggtgaagtga tggctatcgg tcggaatatc gaagagtctt tattgaaagc
    33901 tgtgcgttct ttagaaatcg gtacacacca tatcgaactt cctgagttgg cagacatcac
    33961 ggacgatgac ctaatccaaa aagtggttcg agcacaagat gatcgcctgt tttatgtagc
    34021 tgaagctatt agaagagggt acaccataga agaattagcg gatttgacaa aaatcgattt
    34081 attcttttta gataaactgt tgcatatcgt agaaatcgaa cagcagttga aacagcagcc
    34141 gttggatgac aagatattga aaacagcgaa acaaaacggt tttacagaca aaaaagtggc
    34201 ggagctgtgg gaaataaacg aagaagccgt tcgccagatg agaaaagaaa atgatttgat
    34261 tcctgtatat aagatggttg acacttgtgc agcggaattc gaatcaaaaa caccttattt
    34321 ttatagttcc tatgaatatg aaaatgaaag ttcacgatca gaaaagccct caattctagt
    34381 actgggatct ggtcctatcc gtatcggaca aggggttgaa tttgactatg cgactgtgca
    34441 ttctgtcaaa gcaatccagc aggcaggata cgaagcaatc atcatgaaca gcaatcctga
    34501 aacagtttcg actgactttt ctatttcgga taaattatat tttgagccat tgaccttcga
    34561 agatgtgatg aatgtgatcg agctagaaca gccgatagga gtaatcgttc aatttggtgg
    34621 tcagacagca atcaacttag ccgaaccttt gactgctgct ggtgtaaaaa tcttagggac
    34681 aacgatcgaa gacttggatc gtgcggaaaa tcgtgattta ttcgagcaag cgctgaaaga
    34741 gttggatatt cctcaacctt taggggatac tgctacaagt aaagaagaag ccgtggttat
    34801 cgcttcgaga attggttatc cagttcttgt tcgtccaagc tatgtattag gcggacgtgc
    34861 gatggaaatc gttgaaaacc agcgagattt agaagattac atggaacatg ctgtgaaagc
    34921 atctccagaa catccagtac tagtggaccg ttacttgctt ggtggtgaat gcgaagtgga
    34981 tgcgatttgt gacggtgaaa cagtactcat cccagggatc atggagcata ttgagcgtgc
    35041 tggtgtccat tcaggggatt caatggctgt gtatccacct caaacattat ctgaatcgat
    35101 caagaaaacg atcgaagatt acacgatccg cttagcatta ggtttgaatt gtgtcgggat
    35161 gatgaatatc cagtttgtca tccatgaaaa tgaggtttat gtgatcgaag tcaatcctcg
    35221 agcaagtcgg actgttccat tcttaagtaa ggtgacagga attccaatgg cacaaattgc
    35281 gacgaaagct attttgggag agaaactaaa gacacttggt tatgaaaacg gactttatcc
    35341 agaaacaaaa gaagttcatg ttaaagcacc tgtcttttct tttacgaaat tgcagaaagt
    35401 tgatacttac ttagggcctg aaatgaaatc gactggagaa gtaatgggat ctgacagaca
    35461 attagacaaa gctttgtaca aggcattcga agcttctgga ttacacttgc ctgaatatgg
    35521 agccgtatta tttacgattg cggacgaaac aaaagaagaa gcactagaat tagctaaacg
    35581 ttttacagaa attggctata gtctgatagg aacgaaacat acagctgctt atttcgaaaa
    35641 aaatggtctg gtcgttgaaa cagtagcgaa aatttctgag gaagcagcag aaaaaaatgt
    35701 agtcgactta ataagagatg gtaaaacaca agtggttgtc aatacgattg ataaagatcg
    35761 gggaaatgct tctaaagacg gtttcatcat ccgaagagaa gcagttgaac atggtattcc
    35821 attgtttact tctttagaca cagcagatgc gatcgttcga gtgatggaat cccgtgcttt
    35881 ttcaactaac cctatttaac ggcgagcaca tgttaagaca aaaagacgaa aaaccgttgc
    35941 aggaggggac agatgaaaca agaaatgatg gaaatcgttc agcaaaaaca gctggcacca
    36001 aagatttttg aaatgacatt gaaaggtgat ctagtaaaag aaatgcagca accaggacag
    36061 ttcttgcata ttctcgtgcc gcgtgcagac ttgctattac gccgaccgat tagcttgaat
    36121 catatcgacc acgaaacaaa tacttgccgt atcatttatc gggtggaagg tgaggggact
    36181 agagtcttct ctactctttc tgccggagac catttagatg tcatgggtcc tttaggcaat
    36241 ggttttgatt tgtccaatct tcatacagga gacgaagtat ttataatcgg tggaggaatc
    36301 ggtgtccctc ctctatatga gttatcaaaa cagttagtcg aaaaaggaat taagccaacg
    36361 cattttttag gttttgccac acatgaagtc gtttattatg aaaaagaatt taatcgtcta
    36421 ggagaaacaa gaatcgctac tgacgatgga acatacggta tgcacggaaa tgtcggtaat
    36481 ttattgttag gaacgaagac ggaacctaca gctgtttttg catgtggttc aaacgggtta
    36541 ttgaagacag tggaacagct gttttcttcg catcaaaatg tccagttatc attagaatcc
    36601 cgcatggcat gtgggatagg ggcttgctat gcttgcgttt gtcatacgcc ggaagatgaa
    36661 acaggaacaa aaagtgtgaa agtttgtgac gaaggaccga tttttaaaat aggagaggta
    36721 attatatgaa tgagaatcct ttagcggtca aacttcctgg cctagaatta aaaaatccta
    36781 tcatgccggc aagcggctgt ttcggttttg gcaaagaata cggaaaatac tatgatttga
    36841 atcagcttgg atcgatcatg gtcaaggcaa cgactccaaa tgcccgcttt ggcaatccca
    36901 cgccaagagt ggcggaaaca ccatctggca tgttgaatgc aattggtttg caaaatcctg
    36961 gtctagacgt tgttatgcat aaacttcttc cagaattaga ggagtacaat gagttaccca
    37021 tcatcgcaaa tgttgctggt gcttgtgaag aagactatgt agaagtttgt agcaaaattg
    37081 gcgaagcacc aaatgtgaaa gcaatcgagc tgaacatttc ttgcccaaat gtcaaacatg
    37141 gcggaattgc ttttggaaca gattctgatg ttgcttttca attaacacaa gcagtaaaga
    37201 aagtttcttc tgttcctgtc tatgtcaaat tgtccccaaa tgtaacagac atcgttccaa
    37261 tcgctcaggc aatcgaagct ggaggggcag atgggtttac gatgatcaat actcttcttg
    37321 gtatgcggat tgaccttaag acaagaaaac cgattttagc caatcaaaca ggtggactct
    37381 ccggaccagc aatcaagcct gtcgctattc gtttgattca ccaagtagca agtatatcca
    37441 gccttccaat cattgggatg ggcggcgtgc agacagtgga tgatgttctt gagatgttta
    37501 tggctggagc aagtgctgta gctataggga ccgcgaattt caccgatccg tatatctgtc
    37561 ctaaactaat taaagagctc cctgtgcgaa tgagcgaact aggcatcgaa tcgctggaaa
    37621 gattaatcaa agaagtgaga gaggaacgtg aacgatgaat caactagacc aacgaccaat
    37681 tattgcatta gatttttcaa ctaggcaaga ggtagaagac tttcttcgat ttttcccgaa
    37741 agaagaaaaa ttgtttgtga aaatcggaat ggaattgttc taccaagaag gacctgaaat
    37801 cgtacgttat ctaaaagagg ctggtcataa tgtattttta gatctgaaac ttcacgatat
    37861 tccgaatacg gtcgaaaaag caatgcgggg attggcaaag cttggtgttg atctcacctg
    37921 tgttcatgcc gctggcggta ttcgtatgat ggaggcggca atgcgaggat tagaagaggg
    37981 gactccagaa ggtggaaaac gtccgctact tctagctatc actcagctta cttctacaag
    38041 tgaagaagaa atgcatgccg atcaactaat cgaagtttct ttagaaaaaa gtgtgatcca
    38101 ttatgctagc tgtgccaaaa aagcaggatt agatggtgtg gtttcttctg cttgggaagt
    38161 agaagcgatc aaagaaatgg caggagacga atttgtttgc ttaactccgg gtataagacc
    38221 agaaggaaca gtcgcaggag atcagacaag agtagtcaca ccttctcagg caaaaaagat
    38281 cggctcaact tttatcgtta taggtcgacc aatcactcag tcaactaatc cttataaagc
    38341 atatcaaacg attcaaacag aatggagcta acctaaaatg aatgttgaac aaagtatcgc
    38401 aaaagactta ttagaaattg aagcagtatt tttaaatcca agtaatcctt ttacatgggc
    38461 aagtggaatc aaaagcccaa tttactgtga taaccgaatc acgatgagct atccaaaagt
    38521 ccgaaaagag atcgcaaaag gattagcaag caaaatcaaa gaagcttacc ctgaagtaca
    38581 agtgattgct ggaactgcaa cagcaggaat ccctcatgcc gcatgggtag cggaaatact
    38641 ggatttgcca atggtctata ttcgaagtaa ggcgaaagat catgggaaag gtaaccaaat
    38701 cgaaggacgt atatttgaag gtcagaagat ggttgtgatc gaggatttga tttctacagg
    38761 cggaagtgtc ttggaagcag ctgaagcagc aaaaagagaa ggtgcggata tccttggtgt
    38821 tgcggctatc tttacgtatg agttgccaaa aggaaaagca aatttcgaaa aagccgaaat
    38881 ccctcttatg acgttgacca attattctgt attgattgaa gcagctttag aagatcgtta
    38941 tatcgatgag caagaattaa ctctgctaaa agaatggaaa aaagatcctg aaaattggca
    39001 gatttaagcg cgcttgatga agatagagaa atccgatttg tgtctccaga atcatcaatt
    39061 ggaatagcaa aaatccaaaa actgattgcg agaagcatct tttcatcagg tatgaaaaga
    39121 aacttctatc gtgatcagtt tttgattttt ttaaaattca tttttctaag actggttagc
    39181 agaaagaaga gaataatc
//
LOCUS       DADXTG010000013        39066 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.013, whole genome shotgun sequence.
ACCESSION   DADXTG010000013 DADXTG010000000
VERSION     DADXTG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.013"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            70..3348
                     /locus_tag="IWI57_000542"
     CDS             70..3348
                     /locus_tag="IWI57_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBC2448708.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3889..5796)
                     /locus_tag="IWI57_000543"
     CDS             complement(3889..5796)
                     /locus_tag="IWI57_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBC2448709.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5926..6801
                     /locus_tag="IWI57_000544"
     CDS             5926..6801
                     /locus_tag="IWI57_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBC2448710.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6881..7855
                     /locus_tag="IWI57_000545"
     CDS             6881..7855
                     /locus_tag="IWI57_000545"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBC2448711.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8035..9180
                     /locus_tag="IWI57_000546"
     CDS             8035..9180
                     /locus_tag="IWI57_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBC2448712.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9317..9661
                     /locus_tag="IWI57_000547"
     CDS             9317..9661
                     /locus_tag="IWI57_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBC2448713.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9932..11218
                     /gene="efmA"
                     /locus_tag="IWI57_000548"
     CDS             9932..11218
                     /gene="efmA"
                     /locus_tag="IWI57_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBC2448714.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11356..11955)
                     /locus_tag="IWI57_000549"
     CDS             complement(11356..11955)
                     /locus_tag="IWI57_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBC2448715.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12359..13480
                     /gene="mnmA"
                     /locus_tag="IWI57_000550"
     CDS             12359..13480
                     /gene="mnmA"
                     /locus_tag="IWI57_000550"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBC2448716.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13726..15147
                     /gene="arcD"
                     /locus_tag="IWI57_000551"
     CDS             13726..15147
                     /gene="arcD"
                     /locus_tag="IWI57_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBC2448717.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15161..16279
                     /locus_tag="IWI57_000552"
     CDS             15161..16279
                     /locus_tag="IWI57_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBC2448718.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16339..17631)
                     /locus_tag="IWI57_000553"
     CDS             complement(16339..17631)
                     /locus_tag="IWI57_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBC2448719.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17763..18341
                     /locus_tag="IWI57_000554"
     CDS             17763..18341
                     /locus_tag="IWI57_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2448720.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18470..18847
                     /locus_tag="IWI57_000555"
     CDS             18470..18847
                     /locus_tag="IWI57_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBC2448721.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18780..20852
                     /locus_tag="IWI57_000556"
     CDS             18780..20852
                     /locus_tag="IWI57_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBC2448722.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21063..22010)
                     /locus_tag="IWI57_000557"
     CDS             complement(21063..22010)
                     /locus_tag="IWI57_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2448723.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22256..22720
                     /locus_tag="IWI57_000558"
     CDS             22256..22720
                     /locus_tag="IWI57_000558"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBC2448724.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22782..23825)
                     /locus_tag="IWI57_000559"
     CDS             complement(22782..23825)
                     /locus_tag="IWI57_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBC2448725.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24202..25167)
                     /locus_tag="IWI57_000560"
     CDS             complement(24202..25167)
                     /locus_tag="IWI57_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBC2448726.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25401..26246
                     /locus_tag="IWI57_000561"
     CDS             25401..26246
                     /locus_tag="IWI57_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2448727.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26437..27624
                     /locus_tag="IWI57_000562"
     CDS             26437..27624
                     /locus_tag="IWI57_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBC2448728.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27630..27986
                     /locus_tag="IWI57_000563"
     CDS             27630..27986
                     /locus_tag="IWI57_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBC2448729.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27988..28326
                     /locus_tag="IWI57_000564"
     CDS             27988..28326
                     /locus_tag="IWI57_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBC2448730.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28402..28658
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28721..29755
                     /locus_tag="IWI57_000565"
     CDS             28721..29755
                     /locus_tag="IWI57_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2448731.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29748..30434
                     /locus_tag="IWI57_000566"
     CDS             29748..30434
                     /locus_tag="IWI57_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448732.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30458..31306
                     /locus_tag="IWI57_000567"
     CDS             30458..31306
                     /locus_tag="IWI57_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBC2448733.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31503..32276
                     /gene="sufC"
                     /locus_tag="IWI57_000568"
     CDS             31503..32276
                     /gene="sufC"
                     /locus_tag="IWI57_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBC2448734.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32289..33575
                     /gene="sufD"
                     /locus_tag="IWI57_000569"
     CDS             32289..33575
                     /gene="sufD"
                     /locus_tag="IWI57_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBC2448735.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33572..34807
                     /locus_tag="IWI57_000570"
     CDS             33572..34807
                     /locus_tag="IWI57_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBC2448736.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34794..35264
                     /locus_tag="IWI57_000571"
     CDS             34794..35264
                     /locus_tag="IWI57_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBC2448737.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35269..36660
                     /gene="sufB"
                     /locus_tag="IWI57_000572"
     CDS             35269..36660
                     /gene="sufB"
                     /locus_tag="IWI57_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBC2448738.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36755..37897)
                     /locus_tag="IWI57_000573"
     CDS             complement(36755..37897)
                     /locus_tag="IWI57_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2448739.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37936..38670)
                     /locus_tag="IWI57_000574"
     CDS             complement(37936..38670)
                     /locus_tag="IWI57_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2448740.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38902..>39066
                     /locus_tag="IWI57_000575"
     CDS             38902..>39066
                     /locus_tag="IWI57_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBC2448741.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVG"
BASE COUNT        12667 a         7184 c         8247 g        10968 t
ORIGIN      
        1 ttgacagtaa aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga
       61 ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg
      121 ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg
      181 aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga
      241 actagtgaag atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat
      301 gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga
      361 gaaagcacaa gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct
      421 tccacgaata acggcggaaa attccagtca agcggatcag atggaatgac ctattattat
      481 caagcaatcc ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg
      541 acctatacga atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat
      601 catttgtttg ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa
      661 tatgtctggg actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg
      721 cgacttggat tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg
      781 ccagctcctg ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg
      841 gaaaaagaaa caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc
      901 acgaactcga ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca
      961 tattattacg atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa
     1021 aaattatacg cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg
     1081 acgatcaagg tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg
     1141 caagaaagac ctactcgctt gattgatccg atttacactg tgacctctag taatcattca
     1201 ggaaaccaag atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa
     1261 aataaagcaa caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg
     1321 gaatttgatt atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg
     1381 ccagataaaa actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag
     1441 attcttcata cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa
     1501 gggtcagatg caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga
     1561 tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga
     1621 ttgacgattc caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag
     1681 aaaaatccag aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca
     1741 ctgttcagtc actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa
     1801 aaaggattac agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat
     1861 ggaaatacag gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg
     1921 cataacttag tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc
     1981 gatggatttg cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg
     2041 tatcataatg cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga
     2101 aaagtaatca tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga
     2161 gaaggtaacg gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg
     2221 atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat
     2281 attatcgtca atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc
     2341 tcttcagcag gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat
     2401 cttgagatga gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg
     2461 ttgaactact attggaatga acgagagaaa caatcagaaa atactcttgg acataccata
     2521 tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg
     2581 cgctatgaaa atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt
     2641 caaacagaaa aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga
     2701 gaaacctcac cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca
     2761 aaaccaaatg aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat
     2821 gcagaaggaa atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca
     2881 aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt
     2941 gaaacacagt caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa
     3001 ggagaatata cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct
     3061 gctcatacag aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg
     3121 cccccgggaa atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa
     3181 ccaaatggaa aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact
     3241 caaggactcc caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt
     3301 ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc
     3361 cttttatgat gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata
     3421 gatgtctcac ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc
     3481 tgttcttatt acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa
     3541 tcagctgccc ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt
     3601 tttttctcta atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca
     3661 aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca
     3721 aaatttttct tactacttga aactaagcag ctttttcaca acctctaata catggtattc
     3781 cagaagtaac ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc
     3841 ccaaattcct tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg
     3901 cactcttttt tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata
     3961 gaattcgtca aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc
     4021 gatttgcagt aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca
     4081 attgacaaag aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca
     4141 gcttagaccg cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc
     4201 gtcagcaaaa ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg
     4261 tgtaagatct tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc
     4321 gttattatca aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt
     4381 agcagagctt gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac
     4441 gatcacattc cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc
     4501 tgaacgatcc actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa
     4561 aacaagagta tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc
     4621 gaataatgct gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat
     4681 gacggctttt tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata
     4741 aacggcgatc atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata
     4801 tttttttccg tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc
     4861 tgcttttttc tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct
     4921 gacttggcgg aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat
     4981 ccagcttttg aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta
     5041 cactggatca gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt
     5101 tttcaagtcc agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat
     5161 tttgctgtaa tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa
     5221 ttgcactgct tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa
     5281 taactgaaca gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga
     5341 tgcataatac gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt
     5401 ttgcgcattc ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa
     5461 cagccattct ggaaattgct tattcaattc gtcgaaagac tgcaatgatt caactttttc
     5521 gattataggt tgaagcggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag
     5581 ttgataatat gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag
     5641 cagatcgtct gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc
     5701 gcctgtagct ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc
     5761 ttcatagaaa tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt
     5821 cgtgtgccca ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa
     5881 ttcgaacaat caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg
     5941 cgatcccaac tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt
     6001 aaaaaattag ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct
     6061 ctggtttttg aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat
     6121 tctccagcaa aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat
     6181 gaagagccaa tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc
     6241 gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt
     6301 tttttgaggt ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca
     6361 gcagttctcc ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt
     6421 cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa
     6481 ccactaggac ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag
     6541 aagaatggca atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca
     6601 aaacgtatac gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca
     6661 tatggcaatc attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat
     6721 tacaaagtac acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac
     6781 ggaacagttt cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg
     6841 ttaaactatc gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga
     6901 tgacacgttg aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa
     6961 agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc
     7021 catcaatatc gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc
     7081 acctgtcaat gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag
     7141 tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc
     7201 gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc
     7261 aactcgagta ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc
     7321 tgtagataat ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac
     7381 gggagatgac atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct
     7441 ttcagaatat ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga
     7501 gggtggttat gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt
     7561 gaacacagga gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga
     7621 agtatatgcc tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa
     7681 tgcaccaatc aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc
     7741 agaaaactta acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca
     7801 tgaaaataca ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa
     7861 aaaaagaggg aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt
     7921 ttgtcacagc tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc
     7981 tttggtttgt tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat
     8041 gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt
     8101 gaagcgatga cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt
     8161 ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa
     8221 gcaaaacctc acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc
     8281 ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa
     8341 catcctgctg ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat
     8401 ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa
     8461 gatacgatct tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc
     8521 aaagaaatcg gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa
     8581 gcgtacggaa accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct
     8641 gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat
     8701 atccctgcat tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat
     8761 ttagcaggga tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa
     8821 gcacgtaaca acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac
     8881 gaaatccctt atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt
     8941 cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct
     9001 atttctactg gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca
     9061 atgtatggag aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga
     9121 aataccgaag acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa
     9181 ctagtgaagt cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa
     9241 cttaactgaa aaacctagta aaaatcagga taaactttta taataaagag aaactaattt
     9301 tatcgaggtg aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag
     9361 ctatcgtttg catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac
     9421 aaacggaggc aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg
     9481 attcaaatta aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac
     9541 agcaaatgga ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga
     9601 taaattttat tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg
     9661 atctgtggat ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt
     9721 gtaaggacga cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt
     9781 ttaatatttt gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat
     9841 ttagggaaaa aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct
     9901 atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac
     9961 gaattggaag agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag
    10021 tatggtcgtc caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat
    10081 tttgagtttt gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg
    10141 accgctagtg gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc
    10201 gattttcgcg cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct
    10261 agtttttgtg tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca
    10321 gtcgattatg ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg
    10381 aatggtccaa tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt
    10441 cgttcctgtc aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt
    10501 attgattttt gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat
    10561 ttttaaagat gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat
    10621 aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt
    10681 gatgacgcta gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta
    10741 cgcagtcgga atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg
    10801 aatgaaacct attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt
    10861 ggttccaaac gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg
    10921 tgcaacgcca tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa
    10981 tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg
    11041 attattattt gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc
    11101 cggaatcggg attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta
    11161 tgataaagaa ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta
    11221 tggaatggag ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt
    11281 ggcaataagc ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga
    11341 tatagggcag ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat
    11401 ttgttgctca gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg
    11461 tgcgtctacg cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc
    11521 atgaagattg cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata
    11581 ttgttgatgg tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc
    11641 gtcagtaagc ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt
    11701 ccctttgatc aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt
    11761 tgctttttca tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc
    11821 ttttattact gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa
    11881 gagccgcgga gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact
    11941 gataaagtaa ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta
    12001 tctgagcaac tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc
    12061 gataagaagc aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg
    12121 aaaggctggc cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt
    12181 gattttttgg ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta
    12241 cgacttgata attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa
    12301 aagttgcgac cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat
    12361 gacagacaac agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt
    12421 aacagcactt ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg
    12481 ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa
    12541 agtagctgct caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga
    12601 tcgtgtattt gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt
    12661 gatgtgcaac aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc
    12721 cgattatgta gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca
    12781 catgcttcgt ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca
    12841 agaacagctt tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc
    12901 aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt
    12961 tattggtgaa aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa
    13021 tatggtcact cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat
    13081 tggtcaacgt caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt
    13141 tgtaggaaaa gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc
    13201 attgtatgcc acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc
    13261 aaaagaattc aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt
    13321 tcgcttattg gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac
    13381 tcctggacaa gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga
    13441 ccacgcatat caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa
    13501 acagctatgg ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca
    13561 ataaaattaa tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc
    13621 caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat
    13681 cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg
    13741 acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt
    13801 gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca
    13861 tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa
    13921 aagcgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta
    13981 ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt
    14041 gcaacgatgc tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat
    14101 gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat
    14161 ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta
    14221 ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt
    14281 tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg
    14341 cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt
    14401 gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg
    14461 ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa
    14521 gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga
    14581 aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg
    14641 tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg
    14701 aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca
    14761 atcttgacta atctattctt attgactttc ttagttactg attatgccta tcaatttgcc
    14821 tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc
    14881 cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt
    14941 tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc
    15001 tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg
    15061 ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg
    15121 cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg
    15181 tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc
    15241 gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt
    15301 tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt
    15361 caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa
    15421 tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt
    15481 tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc
    15541 tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga
    15601 agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg
    15661 cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc
    15721 atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat
    15781 cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc
    15841 aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta
    15901 tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt
    15961 gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat
    16021 tttaaaacaa tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc
    16081 tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt
    16141 agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg
    16201 actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt
    16261 cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc
    16321 agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac
    16381 tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac
    16441 aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccac gataaccaaa
    16501 tgcactggaa acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga
    16561 ttggttataa ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc
    16621 ttggacagca gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa
    16681 ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc
    16741 aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc
    16801 tatcaaagtg gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc
    16861 atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga
    16921 tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc
    16981 tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg
    17041 gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt
    17101 agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc
    17161 tgtaacagaa ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc
    17221 agttgcgaca agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg
    17281 caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat
    17341 tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca
    17401 ccaaggagag ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa
    17461 gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat
    17521 gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg
    17581 ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc
    17641 ttctttctga aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca
    17701 atgaaagtct cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt
    17761 ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact
    17821 gctttcgaga actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca
    17881 aacgcttgga tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca
    17941 aaacagcgct aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg
    18001 tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt
    18061 tttcaaatca agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt
    18121 tattagatgg aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat
    18181 taacgaaaga agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc
    18241 tttccttacg ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct
    18301 atttttggat gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta
    18361 acaattttaa gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt
    18421 gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt
    18481 aattaccatg ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat
    18541 tgaagaagaa gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg
    18601 gaacgaaatc catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt
    18661 ttggaatgac ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa
    18721 gggacttgcg ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta
    18781 tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg
    18841 gtgctaatca aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc
    18901 ttggtcattt attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc
    18961 aaatggacac gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag
    19021 gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca
    19081 ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc
    19141 atttcaataa accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa
    19201 ctggattcaa gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg
    19261 cccgggcaaa agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt
    19321 acgcggataa tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc
    19381 aagaaaagaa aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg
    19441 gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag
    19501 cacaaaaact tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa
    19561 aattaaaaac ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca
    19621 atcaacgcta tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga
    19681 cccataagat cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa
    19741 gtacgatgaa agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt
    19801 tgaaaaacgg agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc
    19861 accatgcact tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc
    19921 gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag
    19981 tcagccaaac aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc
    20041 gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg
    20101 aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta
    20161 aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag
    20221 ctacgcgtgc agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc
    20281 aaggattgca agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag
    20341 taacaccaga tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga
    20401 aacaagcagg aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga
    20461 tcaaatcaag caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat
    20521 gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt
    20581 cagattgcaa ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat
    20641 gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg
    20701 atggaacgac agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg
    20761 aagaacgacg cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag
    20821 cagcagcact aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag
    20881 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa
    20941 tttcggctta tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg
    21001 tgacaagact tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta
    21061 tattattggt gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa
    21121 acaggacgct gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga
    21181 cataacaatt ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat
    21241 gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg
    21301 acacaagaca aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg
    21361 ataccatcaa tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca
    21421 cgttcgcggt tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct
    21481 tttgaaaatc ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc
    21541 aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg
    21601 tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg
    21661 gtgccgacac aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg
    21721 ggcggatctt gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat
    21781 ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa
    21841 ggataagctg aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca
    21901 ataaacaaat attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga
    21961 ggaacagttt tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc
    22021 ctttcaatcg tatgtttata tatggtttac attcaaaatt taataaacct taaataacac
    22081 ctttaaaatt tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag
    22141 tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa tttttattag ttggcaagta
    22201 gttttggata gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa
    22261 aatacacaca cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt
    22321 atcttttcag ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca
    22381 agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat
    22441 aaagagaggt acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg
    22501 agaaatccga acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc
    22561 gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat
    22621 cgggcaaaat ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa
    22681 acgccaattg ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca
    22741 ttgttccatg cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa
    22801 ataaatagta aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag
    22861 cgtgtacctt ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca
    22921 gtgatctcaa accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa
    22981 aacaagctgt tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat
    23041 tttcgttttt tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg
    23101 aatttttcaa acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc
    23161 atggcgaatt cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca
    23221 gattggagtg taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt
    23281 aatccaagaa aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg
    23341 atctgtcctt tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa
    23401 gaaccacaaa cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt
    23461 tttttaccaa tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca
    23521 attcctttta caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa
    23581 acagagaatt ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt
    23641 ttaaaaaaag tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac
    23701 tgtggcagat aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa
    23761 ccgggtaaga tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat
    23821 ttcatttgat cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac
    23881 taaaccagta ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg
    23941 tttcgaataa acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
    24001 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttcttttac
    24061 gacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccagtaca
    24121 aaaacatgaa cagctgtttt tgtttggccg ttcttattac ttgatgcaga acagcttttt
    24181 cacaacctat tgaatcgatt attaagcgat ttttttagaa acaaattttc catgtttgct
    24241 atgttcttta attaagaaac gtacgtaatg agcaagaacg tagaagacaa ctacacttgc
    24301 aaacagacat tccgcaactt ttaagaaatc caatacgcta ctatagatac ctaaactgtt
    24361 gaagtaatga acagtagtaa atatagctgt tgcagagata acaagcggga atgtaaaggc
    24421 tgcatagctt ggataaaaac gaagcttgaa caaacgtgga agcatagaca gcacaacgaa
    24481 atacaatact tgagataaag cgaacaatgc tacgacaaga taaaaattag gttcagcaaa
    24541 gtttttaaga taacctgtta ggcaaagaga accaggtgct gcaacgatcg taatcagcgg
    24601 acgtgtcgga tgagccatat ttcttacgaa aaagacacgt ttgatgatga tcggcaataa
    24661 gaacgcatag cagatcaatg aaatccaaaa gaaaattttt ccaataagtg ggaagaagtt
    24721 cccggcagtt acagtgatga ccccaaatcc aacgtaaatg ataaaccagc ttggataaat
    24781 tgcttcaatt gaaagatcag ctttgataac atggtgataa ttgaaataaa agacaaggat
    24841 cacttggaag ataacagcgc acagccaaat gaatttgaca attggtgcaa tactttcata
    24901 tgaaacaaaa taagtacaaa taaccatgag tgacatcgta aaagttggtg aaacggaggc
    24961 gatgattgga tcatgcaacg tttgtttcgt gtgttcgaat aagataaaca atttaacaaa
    25021 aatgaatatc ataagaatca tagagatacc gccagttaga ttgcctaaag caacatggtg
    25081 atagtcttta aacagatttc caagggaagc taacccaagg atcaacccgc agatcgggat
    25141 cggaataatt ttcaaaaagt tcttcatata taatattctc ctttttattt aatagatttt
    25201 agtaccaaga tgaacgaata cattgtaaaa tgaataggaa tgtatgatta cgagcaagca
    25261 gacagctgat tgtcttcatc taagttacta gttggatcag caactgtttt gttgtacatt
    25321 tctattatag atggtttttt gtatatttaa atcaaatagt ttttatagta attatcataa
    25381 aaagttatgg aagtggtgcc atgtttaagt tattaacgac ttttcgagca gtatatgaaa
    25441 caagaaattt ttccagagca gcagaagtac tgtttttatc tcaaccagca gtctcgaatc
    25501 aaatcaaaca gttggagcgg gaattgaata tcgatctttt tcaacgtaac ggaagaaaag
    25561 agatgcttcc aactaagcaa gccgacttat tatatgaacg gacactggtt ttgttagaag
    25621 aatgggaaga aactcagcaa aaattgctca atgaacaaaa tgaagaagaa gtatgtcgga
    25681 tcgttgcttc ccacaccttt gctgtctatc ttttgcctca gttgatgaaa catctgatca
    25741 aaaggtttcc taaagtgcat tttgagatcg aaatcacgaa ttctcatgaa tcattagaac
    25801 aagtagcgaa acatgaatcc gattttggtt ttatcgaaaa gccattagag acttccggcg
    25861 tacaaagata ctctttgatg gaagaccagc tagtcatcgc tggggaccca aaaagcaaat
    25921 tgtggcttgt aagagaagca aactctggtg tttttcatta cacgcagcgt tattttgaag
    25981 agatgaatat ccaagataag aagatgatcg tgaaaagtaa cgatgtaatt gttgcgttgc
    26041 tgaaggaagg aatcggtcgc tcgattttgt ctaagcgctc tgttccagaa aatgtgccaa
    26101 tgactccgtt gaatttccat cgatattttt atttcatcca acgaaccaat ctggtttctg
    26161 atcagctgct agagattgcc gaagagatcc ttcgatacaa tgaaagcaca gaaacttctc
    26221 acagcgagga gaatctcagc tggtagataa ttgcatgtat caaaaaaagt tctatgatac
    26281 actaaagata agcagtaggc ttttgtactg catttgctca agcaactgca caaacggaaa
    26341 gttaattttt cccaaatgtc tatggaaaga ctttccatta tctaactgga ccgctataat
    26401 aatcagcagt atggttttta agaaaaggga tgaaacatgg aaaaaaaatt aaaagcttcg
    26461 aacgataatc gtattgatta tggcgtgatt ttaccagtct ttctgttatg tttgattggt
    26521 ctgctctcat tatatgtagc acttagccat gatccgaaca atccagcagt cttgaaaggc
    26581 gtagggatgc aagttgtctg gtatttagtt ggagcggcag caatcgttgt catcatgcat
    26641 ttgaattcca aatggatctg gaaactgact ccttaccttt atggtttggg gttagtggta
    26701 atgggactct tgctcaaatt ctatgatcag aatttagctg cttcaacagg gtctaaaaac
    26761 tggtttcgat tcggtgcgtt tactttccag ccagcagagt tgatgaagat tgcgtacatc
    26821 ttgatgatgg cactgatcgt aactaaacat aatacgcaag taaaagaacg gacgatgaaa
    26881 tcggactttt ggctgattgg gaagctcttg atcgtgacaa taccggtttt agcattgatc
    26941 atggcccaag atgactttgg aacgatgctt gtctttcttg cgatctttgg cgggattttc
    27001 ctgatgtctg gaatctcttg gcgcatcatc gcacctgtag taatcttggc agtagtcgtt
    27061 ggagcaggaa cgatcttctt ggttacgact gaaggcggaa gagacttgct ttataaagtt
    27121 ggattcaaat cttatcagtt tgcacgtatt gattcatggc tggacccttt ccatgatacg
    27181 tcaggaatga gttatcagcc ggcccaagga cttttggcaa ttggtaccgg tggcttgttt
    27241 ggtaaaggat tcaacgtaag caatatctat gtgcctgttc gtgagtccga tatgattttt
    27301 accgttatcg gtgaaaactt cggctttatc ggtggtgcat tcgtcatttt cttgtatttt
    27361 attttaatct atcgtatgat tcgggtgtgt ttcgatacga ataatgaatt ttacgcctat
    27421 atcgcaagcg gtttgatcat gatgctgctg ttccatgtct ttgagaatat tggagcaaat
    27481 atcggattac ttcctttaac agggatccca ttgcctttca ttagtcaggg aggatcctcg
    27541 attttaggga acatgatcgg tatcgggttg atattatcga tgagatacca aaatgaagca
    27601 cctgcaagat cttcaaggag gtaagaaaat tgtacacctt ttactggtac ccgaaatgtt
    27661 caacatgcaa aaaagccaaa gcgtggttag accagcatgg cgttagttat caaaccgttg
    27721 atatgatcaa agaagtaccg aaggcctcaa cgattgagac ttggctagaa acgagtcaag
    27781 taccaatccg tcgcctgttc aacacaagcg ggatgaaata tcgggaactt ggactgaaag
    27841 atcaagtagg cgagtttacg gcttcaaaag cgagccaagt cttatcgaca gatggtatgc
    27901 tgatcaagcg accactgatc ttacgagagg accagcttgt tgcaattgga ttcaatgaaa
    27961 aaatttatga aggagaactg atataaaatg gcaggcaagc atttgaagaa aaaggacaac
    28021 ctttggatct tatttaatgg aaaagaatac tgtgttggtt tgacaaatga agcgcaagaa
    28081 gaacttggag acatcacgtt tgccaatctg ccaaaagcag gacaatcgct aaaaaaagat
    28141 gaacctctga tcgaagttga agcggaaaaa gcggtcaatg aatttgccag cccgctaacg
    28201 ggtacagttt cttctgttaa tgataaaatc ggtgaagata tcaatatctt gaatgattca
    28261 gatgaaatga atgcgtggat cttaagtttc aaagatgttg atccaaaaga atttgacgaa
    28321 ttataaaatc agaaaatcac ttgaaaaaag ttgacactga ttcttccagt tgctataatc
    28381 ataagcaatc ctatatgaaa cgagaaagct ttgaaaagaa gagtacatgt cacggacgtt
    28441 tgaagagagc cctgtttgct gaaaatgggt aacgaaaatg agatggaaga tggtcttaga
    28501 gcagagtaag cgagcgtaag caaacttact acgggagtgc ccgttacagc gcgacagtat
    28561 acggaaagaa gctaagtcca agtactggaa gaggctgtta ttgcgaagta acggtaaatc
    28621 aaggtggtaa cacggaagtt caagctttcg tccttttgct taaggcaaga gggcgaaggc
    28681 ttttttgttt gaaaaatcat aagagaaaga tgtgaatggc atgtcattaa tccaactaac
    28741 gaatgtcaaa aagacattca atgggaaaaa cggaaacatc caagccgtca atgacgtttc
    28801 actaaacgtt gaaaaagggg atatttatgg aatcgtcggc tattctggcg caggaaaaag
    28861 tacattagtc cgtctcctga atggacttga gcttcccacg agcggcgaag tgatcgtaaa
    28921 cgatcaagat attacaaaat taaaaaacaa agaattacgt acattccgaa agaaaatcgg
    28981 tatgatcttc cagcatttca acctgttatg gtcaagaact gtacttgaaa atatccagct
    29041 gccgctggaa ttagctggcg taccaaagag caaacgtaaa gaacgggcag aagaactgtt
    29101 gcgtttagtc gggttagaag gacgcggaca agcatatcct tcacaacttt ccggcggaca
    29161 gaaacaacgc gtcggtatcg cacgagcatt ggcaaatgat cctgagatcc tgctttgtga
    29221 tgaggctact agcgcattgg acccgcaaac aacagaagaa gtattagact tattgttagc
    29281 gatcaataag aaattgaatt tgaccatcgt cttgatcaca catgaaatga atgtgatccg
    29341 caaaatctgc aacaaagtcg ctgtgatgga gttggggaaa gtcgtagaag aaggagacgt
    29401 attgactgtc ttcagacaac cgaaaaaaga agtaaccaaa cgctttgtac aacaagacat
    29461 cgaaccagaa gctgattcga cagaactatt agctgaactc atcaaagaaa atccaagcgg
    29521 ccgtttggca actttgacct ttagcgaaag aaatgccaac gagccagtca tttcccaagc
    29581 gattcgacgc tataacatcg atgtcaacgt tgtgtatgga aagatcaaac aagtgaaaga
    29641 aggttccttt ggttcattga ctgtcttgat gactggtgaa accaaagaat tagatcaagc
    29701 agaagtgttc atcaaagaac aaggagtagg aattgaggtg atccatcgtg gataaaagct
    29761 tttcagaaac atatctagat tttaaacaaa tcaacccgga aacgatgaat caggctatcc
    29821 ttgatacact atatatgaca ttaatctcaa tggtcttagt gacgatcatc ggattagttt
    29881 tgggattgat cctttactct attggacgca aaaacaaacc gtatgctcga atcatttatg
    29941 gaatcgtatc gatcatcagt aatattttcc ggtcaacgcc atttatgatc ctgatggttt
    30001 tgatcattcc atttactaaa gcaatcgtag gcactatgct aggtgcgcaa gcagccattc
    30061 cagctcttgt tttatccgca gcaccatttt atgcccgtct agtagagatt gcttttcgtg
    30121 aagtcagccc tggtgtactg gaagcagcag atgcaatggg agcaagttac tgggaaatca
    30181 tctggaaggt cttgatacct gaaagtattc ccgcattgat ttctggatta acagttacaa
    30241 cgatctcgat gatcggattt actgcgatgg ccggcgcgat cggcgcaggt ggacttggag
    30301 gtcttgcttg gcaggaaggc taccagcgtg gtaatctaac agtgacattc gttgcgactt
    30361 taattatttt actaatcgta tttatcgttc aaggaatcgg tgatttctta actaagaaaa
    30421 cagacaaaag ataataggat atagggggat gtaggatatg aaaaagaaaa tttttggatt
    30481 tgcagcagcg cttttgctga ctgtaggatt agcagcatgc ggtaacaaca gcgacagcaa
    30541 agattcagca aataaagcag acgacacgac attgaaagtc ggcgcttcac cgacaccgca
    30601 cgcagagatt ttggaacatg tgaaaccatt attgaaagat gaaggaatcg atcttgaaat
    30661 cgtcaaattc gatgactatg tattaccgaa ccaatctttg gaagaaggcg acattgatgc
    30721 caattacttc cagcatattc cttatttgaa caaagaaatc aaagaaaaag gatatgactt
    30781 tgtcaatgcc ggtgctgtcc acatcgaacc aatgggcttg tactctaaaa aagtcaaaga
    30841 tgtttccgaa ttaaaagatg gtgcaacaat cattacttca aattctgaat cagactgggg
    30901 acgcatcatc acaattctac aagatgctgg cttagtcaaa gtcaaagatg gagtagatct
    30961 ggaaacagca acattcgatg atattgaaga aaaccctaag aacttgaagt tcaaccatac
    31021 gatcgaccca tcacttttag cttctacgta ccaaaatgat gaaggtgacc tagttgcaat
    31081 caacgcaaac ttcgcttacg gcgcagggtt gaatccatta gaagatgccg tattgcttga
    31141 aaaggacaac tctccttacg tgaatattat tgcgactcgt aaaggtgatg aagacagtga
    31201 caaaatcaaa aaactgattg aagtgcttca ccgcaaagaa gtccaagaat ggatcacaga
    31261 caaatggggc ggctctgtta aaccagtaaa ggctgatgca aaataaggta tggaacaaaa
    31321 aagatgagaa ataagtctgt aatggcttat ttctcatctt ttttatacct attctaattt
    31381 agaaatcaaa attgtttaat aataaataaa agatatttga ttttagtaag gaaggtcatt
    31441 gcgcttcatt ctcaattagt ctaaaatgag tgagaacgaa agagaatttg ggggtataat
    31501 gcatgtctgt cttagaaata aaaaatctac atgtgtcgat cgaggaaaaa gaaatcctta
    31561 aaggcgtaaa cctaacaatg aaaaccggag aaatccatgc catcatggga cctaacggca
    31621 caggaaaatc taccttatct gccgctatca tgggcaatcc aaactatgaa gtaacagaag
    31681 gcgaaatttt gtttgacggg aaaaatgttt tagacttgga agtagatgaa cgtgcgcgac
    31741 ttggcttatt tttagctatg caatatccaa gtgagatacc aggtatcacg aatgcggaat
    31801 tcatgcgtgc agcgatcaat gcgaaacgtg atgaaaacga caaaatttct gttatggcat
    31861 ttttgaaaaa gttagatgaa aaaatggctt tactaaatat gccagaagaa atggctgaac
    31921 gttatctgaa cgaaggtttt tctggcggag aaaagaaacg gaatgagatc ctgcaattgt
    31981 tgatgttaga acctacattt gcaattttag acgaaatcga ttcaggtctg gatatcgatg
    32041 ctttgaaagt cgttgctaaa ggagtaaatg aaatgcgcgg agacaatttc ggtgcattga
    32101 tcatcaccca ctatcaacga ttattgaact atatcacacc tgatgtcgtt cacatcatga
    32161 tggaaggccg agtagtcatg acagggaatg ctgacctagc taaacgtctg gaagcagaag
    32221 gctacgctgg tatcagtaaa gaattaggaa tcgactataa agaagaagaa gcgtaagggg
    32281 gagcaagaat gaaagaaaaa aactggctcg attatcttga cgcggtaaat gatttttctt
    32341 taagtaaagg cgaacctgat tggatgagaa ccttccgtca agacgcacta gcgaaagcag
    32401 atgaactgcc attgccgcat atcgatcggg tgaaattcca tcgctggtcg ttatttgatg
    32461 tgaaagaaac acaaaccatc agtgaaacag gcacgattcc ggcatttgat gcaatgaaag
    32521 acaatcctgt gcttgtccaa caaggttcat ggacgatttt tgaacaactg cctgtggaat
    32581 tagcagaaaa aggagtcatt tttaccgact tgttcactgc aatgatcgaa tatccagaac
    32641 tagtccaaga atattatatg aaaaaagcag taaacatgaa cgaagaccaa ttgactgcac
    32701 tccatgtagc ttttatgaac agcggtattt tcctgtatgt gcctaaaaat gtcgtgatcg
    32761 atgaaccact cgagtcctta ttcattcaag atggcgcatc cgatgagcac ttcttcaagc
    32821 atgtgttgat cgtggcagat gaacatagtg aattttctta tctggaacgt ttccagacaa
    32881 ccaaagaaca agtagcaaaa tcatcaggta atatcatcgt ggaagtcatc gctaaagctg
    32941 gttcaaaaat caaatattca gccgtagatc aattaggaga aaatattact tcttatatga
    33001 atcgccgtgg gcacatttta cgagatgctt ctgtcgactg ggcaattggt gtcatgaatg
    33061 acggtcatgt gatcgctgac tttgactcag atttagcagg agaaggtgca cacgcagaag
    33121 tcaaaatcgt ggcaatcagc tcgggcagac aaatccaagg aatcgataca cgagtaacca
    33181 ataaagcccc tcatacgatc gggcatattt tgcaacacgg agtgatccga gaaaaaggaa
    33241 cattgacctt taatggtatc ggccacatct tgaaaggggc aaaaggtgca gatgcccaac
    33301 aagaaagtcg tgtcttgatg ctttctgata aagcacgtgg tgatgcgaat cctattcttt
    33361 tgatcgatga aaacgaagta actgctggac atgctgccag tgtaggacga gttgatccag
    33421 aagaaatgta ctatctgatg agtagaggat tgcataaaga agaagcagag cgcttagtga
    33481 ttcgaggatt cctcggttct gtcttaacag caatccctgt agaacaagtt cggaaagaat
    33541 tagtcgaagt aatcgaaggg aagttgaatg gatgatcgat gctaaaaagc tacgcaatga
    33601 ttttcctatc ttatttcaag acgtcaatga tgaaccttta gtttatctgg ataatgcagc
    33661 gacgacccaa aagccaaaac aagtactcca ggcattagcg tactattacg aacacgataa
    33721 cgccaatgtt catcgcggtg tccatacgtt agcagaacgc gcgacccatg attatgaaca
    33781 agcaagagaa aaagtacggg cgttcatcca tgcaaaagaa acagcagaag tgctgtttac
    33841 tcgtggtacg acgacaagct tgaattgggt agccagaacc ttcggcgacc gctatatcaa
    33901 agcaggagac gagatagtgc tctcttacat ggaacatcat tctaatgtta tcccgtggca
    33961 gcagttggca aaacgaacag gtgctgtctt gaaatatatc gaactaacag aagaaggctt
    34021 cctggatatg gaggatgcag cgaaaaagat tacggacaaa accaaaatcg tctccattgc
    34081 tcatgtatcc aatgtcttag gtgtcttcaa tcctgttcaa gaattagctg aacttgtcca
    34141 tgaaaacggc ggggtcatcg tagtagatgg tgctcaatct gccccgcaca tggccattga
    34201 tgtccaagca ctagactgtg atttctttgc tttcagcgga cacaaaatgt gcggaccaac
    34261 aggtattggt gttttatatg gtaaacgaaa atggttagaa gaaatggaac ctgttgaatt
    34321 tggtggcgaa atgatcgatt ttgtcgaatt atacgacagt acatggaaag aactaccttg
    34381 gaaattcgaa gcagggacac caaatatcgc cggcgctatc gcattaggcc atgcaatcga
    34441 ttatttgcaa aaactcggta tggacaatat ccatcgatat gaagaagaac tagccgctta
    34501 tgtgttgcca aaattacaag caattgatgg attgactgtt tatggtccac aagacccgaa
    34561 ttttcggaca ggagtcgttg cattcaattt ggatgggtta catccccatg atgtcgcgac
    34621 agctttagac atggaagggg tagccgtcag agcaggtcat cattgtgcgc aaccgctatt
    34681 gaagtacctt gaagtatcag cgactgctcg cgcgagcttt tatttctaca acacaacgca
    34741 agacgccgac cgtttagttg aagcaatcat ggcaacaaag gagtttttcc aacatgggac
    34801 tatctaaatt agacaattta taccgtcaag tgatcttgga tcattcttcc catccgcatc
    34861 atcgtggaag cttgactgac tcgacaagca agatcgaatt gaacaacccc acatgtggag
    34921 atgtgatcga attgcaagtc gatgtaaaag acggaatcat caaaaatatc gcctttgacg
    34981 gcagtggctg ctccatcagt acggcaagtg cctcgatgat gacagacgcc gtcatcggaa
    35041 aatcaataga agaagcagaa catttaactg tagactttct acagcttgtc caaggaaaag
    35101 aagtcaaaga tcccgaccaa ttaggcgatg ctgctatgct tggtggtgta gctaaatttc
    35161 ccgctcggat caaatgtgcg acacttgcct ggaaagcact aggaaaagca atggaaaacg
    35221 atggtaccgc agctttccat cattaccaca aggaagagga gtgagaacat gggcgtacca
    35281 gaattagaag aatataaatt cgggtttcac gatgatgtcg aaccggtttt cagtacagga
    35341 gaaggcttga cagaagaagt gatccgtgag atgtcaagaa tcaaaggcga accagaatgg
    35401 atgcttgaat tccgtctgaa atctctagaa acatttaaca aaatgccaat gcaaacatgg
    35461 ggccccgacc tttcagacat cgatttcgat gccatcaaat attaccaaaa accaagtgac
    35521 aaacccgctc gtgactggga agacgtacca gaaaaaatca aagatacatt tgaaaagatt
    35581 ggtataccag aagctgaacg agcttatcta gccggtgctt ccgcacaata tgaatcagaa
    35641 gtcgtgtacc ataatatgaa agatgaattc gaaaagttag gaatcgtctt tactgacact
    35701 gactctgctt tgaaagaata ccctgaatta ttcaaagagt acttttcaaa acttgtaccg
    35761 ccaaccgaca acaaattagc agcgctgaat tcagccgtat ggtcaggtgg gacctttatt
    35821 tacgtgccaa aaggcgtcaa agtcgatgtt ccattacaaa cgtacttccg gatcaatgcg
    35881 gaaaacacgg ggcaattcga acgtacgctg atcattgttg acgaaggagc aagcatccac
    35941 tatgtcgaag gttgtacggc acctacttat tcaagtaaca gcctgcatgc cgccatcgta
    36001 gaaatcttta caagaaaaga tgcgtattgc cgttacacaa cgatccaaaa ctggtcagac
    36061 aatgtgtaca acttagtaac caaacgagca aaagcatacg aaggcgcaac cgttgaatgg
    36121 atcgacggaa acctaggtgc aaaaacaacg atgaaatacc caagtgttta cttagatgga
    36181 aaaggtgcac gaggcaccat gctctccatc gcctttgcag gagcaaacca aatccaagat
    36241 acgggtgcaa aaatgatcca caatgcacca aatacatcta gctctatcgt ctctaaatca
    36301 atttcaaaag acggcggaga agtaaactat cgaggacaag taacctttgc caaaaacagt
    36361 gcgggttcga tctctcatat cgaatgtgac acgatcatca tggacgaatg gtcgaaatcc
    36421 gatacgatcc cattcaatga gatccacaac agccaagtct ctttagaaca cgaagccaaa
    36481 gtctcaaaaa tctcagaaga acaactctac tatctgatga gccgaggcct gaccgaatct
    36541 gaagcaactg aaatgatcgt catgggcttt gttgaaccat tcaccaaaga acttccaatg
    36601 gaatatgcag tcgaactcaa tcgattgatc agttatgaga tggaaggcag tgttgggtaa
    36661 acctgttata ggagctagaa cccttgatat ataagggttc tagctttttt gttttgcctg
    36721 tatactagaa atgaccagat tttgaccagc tcttttactg atgagccatc atatcagcaa
    36781 agtcttgacc tgttttctta ggtgcattat ttgtaatatg tgcatagatg tctaatgtca
    36841 ctttactatt ttcatgccct aatcgttgtt gaactacctt gacagattct cctgcttcta
    36901 ataataaact agcatgtgta tgtctaaacc catgaggtgt aatacgaggc aagttatatt
    36961 ttttcaaaat atatcttaac caatcattaa cagtgtttgg aatgtataga gtgtttttaa
    37021 ctgttgtaaa aacatgttgc tctttagagc tagtattgta tccaagtttt aaatactctt
    37081 ctttttgctg taatttccag ttgttgagga tagaaagagt ctcagtatct aaagagataa
    37141 ctcgtctaga agctcttgtt ttcggagatt gaattataac tttaccaaat tcatctacag
    37201 caagagtttt attgacatgt agttctttat taaacgtgtc tatgtctttc cactgtaaag
    37261 cgagtacttc actctttctc atacctgtgt atgccaataa tctgaagaag gcgtattgtt
    37321 tcatgttacc aaaatcttta aaagcatcca gtaaattatt aagctcctct tttgagtaaa
    37381 atttacgatt tttctcatac tctttttttc gtggaagaag tgttttttcc ataggattat
    37441 tgtcaatata ttgcattgca accccgtagc gtaaaatttg tgcagtctgt tttctcatat
    37501 aatcgtaact ttcatattta ctgtgccatt catctaccac tttttgacaa tatgggacag
    37561 agatattcga tagtttaagc ttaccaaatg ctggaagtat ttgattattt gcgtatctta
    37621 ctgagattgc gattgttgaa gctttaacac tggtacgctg atgagttatc cattcattat
    37681 acaggtcttc aaatgtcata tcaggtgctt tctttttaac accgtataat atttgatgtc
    37741 tgtactcgtc ataatctttt tgagcttctt gtttgctact aaaacctctt ctcgtagtat
    37801 ttattttttc accagttcgt ggatcggtgg caatgtgtcc tttatacatc cacgccactt
    37861 gaccgttttt ctttttgtac tttgatattt ttgccattaa atcatctcct cttagtttac
    37921 aaggagtact taccactaga tggtatcacc tcccttcatt tttttcttag tgtatttttt
    37981 cccaattgat gaaatcgttt gttctagctt gtttatatta tcagtaattt ctctcacagc
    38041 ttcttcggga taagacatgt ctttcaattg aataggtagc cattcatcta ccattctttc
    38101 tagtagttct tctatactgc tagtgaggaa aagaatgtta ttgtcaaatt cttctacaat
    38161 agcattgatg aataattctt ctatttttaa gatattgaaa agactaattt tttcattttt
    38221 aactttatta caagtcttgt tagcaataaa ttcttgcatc tctggttcta atttggaata
    38281 tctatccagc catgtatcta aattagagtg tactggtggg aactgttgac gaagatgctg
    38341 gaacaggttt ttatctggaa atgttatacc atctagtaca atgctatctc tataaagaat
    38401 aattcccaaa atatattctt ctacattgcc acaaactaac tcctctatac tcatatttcc
    38461 caaatcagca attcgggata atcgctctgg tattggtaaa taggttccct tctcccattc
    38521 gtagatagtt tttttgtcaa ttaattgacc gttgttaatc ataagaccaa atgctggttg
    38581 tgttaatttt ttactctttc ttatagcttt tatttttgtt ccgagtatct tttttcgttc
    38641 taaataattt attggtggac gttttatcat attgaccaca cctcctaaaa ttagtatacc
    38701 tcaaaaaagg ggtgttgtaa ataaatttta caaagctgtt gacacccctt ttttatcgag
    38761 gtattatgtg tatgtcgatg aactggtaat cattttttgt ttgattaatc ggttaataac
    38821 aaaatacttt tattttgagc aaaaaatacg aagataattc ttagaaaatt tgttcaagca
    38881 atcacgaaag gagggggaaa tatgtctcta caaatcattg atagcattgt ttctatacaa
    38941 gttgaacaac aagtgaaaga gataattaca gtgctaaagc ctatgctaga agacattatt
    39001 tctggagtca ctctagagta tatggactat cagcaggcaa gtgaatatct aggtgtatca
    39061 gtagga
//
LOCUS       DADXTG010000014        36755 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.014, whole genome shotgun sequence.
ACCESSION   DADXTG010000014 DADXTG010000000
VERSION     DADXTG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.014"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(293..988)
                     /locus_tag="IWI57_000576"
     CDS             complement(293..988)
                     /locus_tag="IWI57_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBC2448742.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(1078..2178)
                     /gene="ychF"
                     /locus_tag="IWI57_000577"
     CDS             complement(1078..2178)
                     /gene="ychF"
                     /locus_tag="IWI57_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBC2448743.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2187..2378)
                     /locus_tag="IWI57_000578"
     CDS             complement(2187..2378)
                     /locus_tag="IWI57_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBC2448744.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2399..3289)
                     /locus_tag="IWI57_000579"
     CDS             complement(2399..3289)
                     /locus_tag="IWI57_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBC2448745.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3276..4043)
                     /locus_tag="IWI57_000580"
     CDS             complement(3276..4043)
                     /locus_tag="IWI57_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBC2448746.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(4055..4768)
                     /gene="rsmG"
                     /locus_tag="IWI57_000581"
     CDS             complement(4055..4768)
                     /gene="rsmG"
                     /locus_tag="IWI57_000581"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBC2448747.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(5000..6901)
                     /gene="mnmG"
                     /locus_tag="IWI57_000582"
     CDS             complement(5000..6901)
                     /gene="mnmG"
                     /locus_tag="IWI57_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBC2448748.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6918..8315)
                     /gene="mnmE"
                     /locus_tag="IWI57_000583"
     CDS             complement(6918..8315)
                     /gene="mnmE"
                     /locus_tag="IWI57_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBC2448749.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8614..8997
                     /locus_tag="IWI57_000584"
     CDS             8614..8997
                     /locus_tag="IWI57_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448750.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9165..9923)
                     /locus_tag="IWI57_000585"
     CDS             complement(9165..9923)
                     /locus_tag="IWI57_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBC2448751.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9941..10759)
                     /locus_tag="IWI57_000586"
     CDS             complement(9941..10759)
                     /locus_tag="IWI57_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBC2448752.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10756..11112)
                     /gene="rnpA"
                     /locus_tag="IWI57_000587"
     CDS             complement(10756..11112)
                     /gene="rnpA"
                     /locus_tag="IWI57_000587"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBC2448753.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11230..11364)
                     /gene="rpmH"
                     /locus_tag="IWI57_000588"
     CDS             complement(11230..11364)
                     /gene="rpmH"
                     /locus_tag="IWI57_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBC2448754.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11887..13221
                     /gene="dnaA"
                     /locus_tag="IWI57_000589"
     CDS             11887..13221
                     /gene="dnaA"
                     /locus_tag="IWI57_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBC2448755.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13419..14549
                     /gene="dnaN"
                     /locus_tag="IWI57_000590"
     CDS             13419..14549
                     /gene="dnaN"
                     /locus_tag="IWI57_000590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBC2448756.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14778..15020
                     /gene="yaaA"
                     /locus_tag="IWI57_000591"
     CDS             14778..15020
                     /gene="yaaA"
                     /locus_tag="IWI57_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBC2448757.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            15007..16131
                     /gene="recF"
                     /locus_tag="IWI57_000592"
     CDS             15007..16131
                     /gene="recF"
                     /locus_tag="IWI57_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBC2448758.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            16128..18074
                     /gene="gyrB"
                     /locus_tag="IWI57_000593"
     CDS             16128..18074
                     /gene="gyrB"
                     /locus_tag="IWI57_000593"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBC2448759.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            18094..20565
                     /gene="gyrA"
                     /locus_tag="IWI57_000594"
     CDS             18094..20565
                     /gene="gyrA"
                     /locus_tag="IWI57_000594"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBC2448760.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20801..21100
                     /locus_tag="IWI57_000595"
     CDS             20801..21100
                     /locus_tag="IWI57_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBC2448761.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            21150..21662
                     /gene="ssb"
                     /locus_tag="IWI57_000596"
     CDS             21150..21662
                     /gene="ssb"
                     /locus_tag="IWI57_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBC2448762.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21688..21924
                     /locus_tag="IWI57_000597"
     CDS             21688..21924
                     /locus_tag="IWI57_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBC2448763.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            22067..24040
                     /locus_tag="IWI57_000598"
     CDS             22067..24040
                     /locus_tag="IWI57_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBC2448764.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            24046..24498
                     /gene="rplI"
                     /locus_tag="IWI57_000599"
     CDS             24046..24498
                     /gene="rplI"
                     /locus_tag="IWI57_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBC2448765.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24772..26139
                     /gene="dnaB"
                     /locus_tag="IWI57_000600"
     CDS             24772..26139
                     /gene="dnaB"
                     /locus_tag="IWI57_000600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBC2448766.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26919..28211
                     /locus_tag="IWI57_000601"
     CDS             26919..28211
                     /locus_tag="IWI57_000601"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBC2448767.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            28998..29861
                     /locus_tag="IWI57_000602"
     CDS             28998..29861
                     /locus_tag="IWI57_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBC2448768.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(29990..31612)
                     /locus_tag="IWI57_000603"
     CDS             complement(29990..31612)
                     /locus_tag="IWI57_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2448769.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            31861..32382
                     /locus_tag="IWI57_000604"
     CDS             31861..32382
                     /locus_tag="IWI57_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBC2448770.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(32568..34298)
                     /gene="spxB"
                     /locus_tag="IWI57_000605"
     CDS             complement(32568..34298)
                     /gene="spxB"
                     /locus_tag="IWI57_000605"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBC2448771.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            34463..35281
                     /locus_tag="IWI57_000606"
     CDS             34463..35281
                     /locus_tag="IWI57_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBC2448772.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(35384..35581)
                     /locus_tag="IWI57_000607"
     CDS             complement(35384..35581)
                     /locus_tag="IWI57_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448773.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            35750..36328
                     /locus_tag="IWI57_000608"
     CDS             35750..36328
                     /locus_tag="IWI57_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBC2448774.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            36423..36560
                     /locus_tag="IWI57_000609"
     CDS             36423..36560
                     /locus_tag="IWI57_000609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448775.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT        11159 a         7068 c         7298 g        11230 t
ORIGIN      
        1 ggttgggttt attaccgcag atgcaaactt ttgaacaccg tttagtggag aggctgtgaa
       61 aaagctgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc cacatttttg
      121 cataaattgg acttaatacc gagaaactgg cttttgaaca ccgtttatcg taaaaaggga
      181 ctgtgacaag acttttgtca cagtccctta ctattattta actatggata aacaaaatga
      241 ggttctttac tacccctaga tatctagcaa aatagctgat ctttttgcta gtttatcttc
      301 ttgggacgga taaacttcca ataatcccgt attttttctt caagtagtaa cggattgcta
      361 aagcagttgc cccgataatg ataactacta ctggatctaa tactggattg ataactggtg
      421 gcaatagtgt tgtcgcagaa aacagtacga cccaaagaag tgtcgttcca cctaggataa
      481 gcaatgtctt acccattcct ggtttcttgc ttcgatctgc tccgggatat tcatattgat
      541 agatgtattt gtacatcata taaaagaccc agccaccgat tgcagaacct agaacgagtg
      601 tagaaaagcc ataagctggc gttgttccac gtgaaaacaa tccggtaatc gcaatcatga
      661 tacttaatag accaagcaac agcaacgtat tatctaacca catcaataaa ggggtggatg
      721 aattttttac ttctgctggc ttattgatga ttgcttccgt tctttcagag actgtcccaa
      781 acaattgacg ggctgttctt ccgccttttt gttctgcaac taaagtaggc agaatgtcat
      841 gaagggcttt tactttttct tcttcagaca aattcgctgc ttctaaagat tttttcaaat
      901 caaaaatgta ctgttgattt cttttggtta attttgtttc taaactattg ttttctttta
      961 cgattgctcg taattgttct ggttccatta tctaatagtc ctccttagcc gttccttttc
     1021 tcaacggtcg tcacaaaaga cattctacca tattctacag ctagtttgat agccctttta
     1081 cacattaaag cggaataaca ttacgtcgcc gtcttgtacg acataatctt ttccttctaa
     1141 tcgaacacgt ccagcttctt ttgctgcatg catgtttccg taatgatcca agtcttcaaa
     1201 agagacagtt tctgcacgga taaatccgcg ttcgaaatct gaatggatga ttcctgctgc
     1261 ttgtggtgct ttgattcctt ttttgaatgt ccaagcacgt acttcttgtt ccccagcagt
     1321 aaagtaagta gctaagccta acaaatcata agcagcacgg atcaactgat ccaaacctga
     1381 ttcttcgatt cctaaagctt ctaaaaattc cgctttgtct tcttcatcta attctgcgat
     1441 ttcttcttct gcacgagcac tgaccacgat cacttctgca ttttcttctg cagcaaagtt
     1501 gcgcacttct tgtacataat gattcgcgtc tgcatctgct acgtcatctt ctgagacatt
     1561 ggctacataa agaattggtt ttgtcgtcaa taaaaatagt gatttaacga ttttttgttc
     1621 ttcttcagta aattcgatcg ttcgggcaga ttttccttct tccagtacag gtttgatttt
     1681 atccagtaca gctagttctg ccactgcatc tttatctttg gttttagcaa cttttgctac
     1741 acgagtatag cgtttgttga tagattctaa gtctgctaac actagttcca agttgatcgt
     1801 atcaatatct tctagtggat caacacgtcc ttctacgtga gtaatattat catcatcaaa
     1861 acagcgaaca acgtgacaaa tagcgtctac ttggcggata tggcttaaga attgatttcc
     1921 caaaccttct cctttacttg cgccttttac gattccagca atatctgtaa attcaaaagt
     1981 cgtcggaaca gtttttttcg gatgtacaag ttctgttaag cgttgcaaac gccagtcagg
     2041 tacttctacc atccctacgt tggggtcgat cgtcgcaaag ggatagtttg ctgcttctgc
     2101 tcctgctttt gtgatcgcat taaacaaagt tgattttcct acgttgggca acccaacaat
     2161 tccagcagtc aatgccataa atttattcac tctttctctg catttttttc aattactttt
     2221 tttaatttct tttcaaaatc tcgacgggtc atcatgacga tatgtccgca attcatacat
     2281 ctgattttga tatctgcgcc cattcgaatg acttcccagc gattcgtctg gcaagcgtga
     2341 ggttttttca tttcaacgat atcgtgcaag tcgtacataa aaatagcctc ctttttgttt
     2401 attcttcatc aaattgaata ttcaagatat caagaattcg agtcaaatct ttttgagaaa
     2461 gatattcaat ctcgatctta cctttcccat ttttttcttg gatagctaca cttgtaccaa
     2521 atttatccat caaacgatct tcgctttctc ggatataata tggtttttcc tttgctagcc
     2581 ttgggatttt tttcttttct tttccatcat tttgatttaa ctcagaaaca agctgttcca
     2641 gttggcggac agttaaattt tcagttactg cacgattggc taatttcaag atcaatgttt
     2701 tattttttaa acctaacagc gtacgggctt gtcccataga taaacgctga tcttgtacca
     2761 tttccttgac aagatccggt aaagaaagca agcgcaaata attggcgata taaggtctgc
     2821 tctttcctaa acgactagct acttctgctt gtgttagttt caggtttttc atcagcatct
     2881 catatgcttc tgcttcttct aacggattca gatcttctcg ctgcaagttt tccagcactg
     2941 ctacttgcat catcgcttct tcgtcaaagt ctctaacgat agcagggatc gtttcttttt
     3001 cagctagttt ggacgctcga aaacgtcgct cccctgcaat aatctcatat ccttttactg
     3061 cggaacggcg tacgatgatg ggttgaaaga cacctgattg ttcaatagaa ttagctagtt
     3121 cctgcaaaga tacttcttca aatgttttac ggggctgata cggatttggt cgcagctcat
     3181 tcaatggtag ctggacaacg gtatcatctt ttacatccac cgtctctaag ttttctaaat
     3241 cttgaaacag tgcatcgatt cctcggccta atcctttact tttcttcacg acttaccact
     3301 tcctttgcca atgcttggta gacttcagct cctcgagaac gtggatcgta gtcgatgatc
     3361 ggttttccat gactaggtgc ttctgataaa cgaatatttc ttggaatgat cgtttcgtaa
     3421 actttttcgc ggaagtattt acgtacttct tctacgactt catttcccag atttgttcgt
     3481 gcatcataca ttgtcagtaa gactccctcg atctctaatt ctggattaaa gtgtttttgg
     3541 actaaacgaa ccgtattcag taattggctt aatccttcca atgcgtaata ctcacactga
     3601 acaggtatca aaatcgaatc acttgctgta aatgaattga ttgtcagatg gccaagagaa
     3661 ggcgggcaat cgatcaaaat ataatcatac tgactgctta cttctgctaa agatcctttt
     3721 aatcttgatt ctcttgccat cattgacgtc aattcgatct ctgctcctgc taattggaga
     3781 gtcgcaggca caatactcaa attttcgtgt tcagtgatca aagttgcctc atcaattggc
     3841 aattcattga ctagaacatc atagatgtct cttgtcacat caggttttct gatcccgaca
     3901 ccactggtag catttccttg tgcatccata tccactagca atactttttt gccaaggcta
     3961 gcaagacaag cacctagatt caccgtcgtc gttgttttgc cgacgccacc tttttggttc
     4021 gcaacagata ttatttgtgc catctcttct cctcctattt gattggctgc ttattaggca
     4081 gtccgggctt tcttgggtat ttctttgggg ttggtttctt tttttgaata acgatgatat
     4141 gccgttgatc agagacattc ggcaaggtaa ccgcctcttc tttgaggaat ttaccaccta
     4201 ataaagcaat cgcttgtttt gcttctgtca attcctcttc actttttgct gctttcaatg
     4261 cataaaaata gccttcttgt ttagccaagg gcaaacacaa ttccgctaac acattcaaac
     4321 gagcaaccgc acgagcagtg acaaggtcaa atgatgcacg aaagtgagga ttttgcccaa
     4381 atgtttctgc ccggtcatga tataaggaag catcgattcc taataatcgg gttaactcag
     4441 ataagaaggt tattcgttta ttcagagaat caacgatcgt tatctgcagt tctggaaagg
     4501 caatttttaa aggtatgcta ggaaaccctg cccccgaacc aacatcgcat aaagataaag
     4561 gttgcgtaaa gtctacttca aatgctaatg taagtgaatc gtaaaaatgc ttcagatata
     4621 cgtctttttc atccgtaata gctgtaaggt tcatcttttc gttccattcg actaatgtcc
     4681 gataataaat agcgaattga ttcatttgat gatcggtaag ctctatccct ttttctgcca
     4741 aggattggcg aaattcttct ggtatcataa atattccttt cattgtgaaa cagcaattgt
     4801 ttcacagatt ctctccctac tttaacgcaa aatctctaga catgcaatcc ttccatatgg
     4861 attcttaaaa atttgaaaag taagaaaagc cgatagactt tttctttcta gtatgactca
     4921 taaaatatac acagaagaga gaactagctt tgagcaattt ctgcaaaaac tagttctctc
     4981 ttctatttat tattacgtat tacgctggtt cactgacttt cgcaatcttc ccttgttcga
     5041 tatagaccat taggatactt aggtctgcag ggtttacgcc actgattcgg ctagcttggg
     5101 caattgtttc tggttggatt ttctgcaatt tttgttttgc ttctgttgct aatccgttga
     5161 ttgctgcgta gtcgatgttt tctggaatct ttttcgcttc catgcgtttt aatttttcta
     5221 ctttttccat ggcttttttg atgtagcctt catatttgat ttggatttct acttgttcga
     5281 tttcttttcg tgtcaattct tcattttctg gaataaaccg tagaagttct gcataagtaa
     5341 tctcaggacg tcttaaaaag tcgcttgcta gtacaccatc ttttaatggt gccgcattaa
     5401 tcgttgctaa aaagtcttgg acttcttctg ttggtttgat tcgtacattc atcaaacgtt
     5461 tgatttcttg ttcaactgct tcttttttct tcaaataatt cttgtattga tcttctttta
     5521 ctaagcctac acggtgaccc atttctgtta atcgcaaatc tgcattatca tgacgtaaaa
     5581 gcagacggta ttcagcacgt gaagtcagca aacgataagg ttcgttcgtt ccttttgtta
     5641 ccaaatcatc gatcatgaca ccgatgtaac cgtcacttcg tttgatcact aaaggttctt
     5701 ttccctgaac tttcaaagct gcattgatac cggcaatcag cccttgtcca gccgcttctt
     5761 cataaccgct tgtaccattt gtttgtccag ctgtataaag attttcaacg actttagttt
     5821 ctaatgtcgg acggagctgg tgtggctcta ctacgtcata ttcgatagca tatcctgtac
     5881 gcatcatttc tactttttct aatccagcaa tagaatgaag catgttgact tgaacatcct
     5941 ctggtaaaga agtagataaa ccttgaacat agacttcttc cgtatttaag ccttctggtt
     6001 ccaaaaataa ttgatgacgt ggtttgtctg caaaacgcac gatcttatct tcgatggatg
     6061 gacaatagcg agcgcctacc ccttcaacga tccctgtaaa cattggggca cgatgtaagt
     6121 tgtctcgaat aatttggtga gtcgtctcac ctgtataggt cagccagcaa gattcctgat
     6181 ctagtttata ggcactatct ggtgtgctga agctaaaatg attcggctct ttgtcaccag
     6241 gctgctcttc tgtaacagaa tagtcgatcg tacttgattt tactcgaggc ggtgttcctg
     6301 ttttgaaccg ttcgatagca aagccaagtt cttttagatg attcgctagt ccaatagaag
     6361 gttgagagtt attcggacct gatgaatatt tcaattcacc gataatgatt tctccgcgaa
     6421 gtgctgttcc ggctgtaata acaactgctt tggcccggta gctcgctcct gtggacgtca
     6481 ctactccatg acaaacacca tcttcgacga tcagttcttc gacgattcct tgtcttagcg
     6541 tcaaatttgg ttctttttcg atcgtatgtt tcatttctgt tgaataagca tgtttgtctg
     6601 cttgtgctcg caatgctcgg acagctgggc cttttcctgt gttcaacata cgcatttgga
     6661 tataggtttt atcgatattc ttacccattt ctccgcctaa cgcatcgatt tctcgaacga
     6721 caacgccttt agctggtccg cctacagaag gattacatgg catgaatgct accatgtcca
     6781 aatttattgt cagtaataat gtttttgatc ccatacgtgc agcagcaagt gcagcttctg
     6841 aaccggcgtg tcctgcgcca acaacgatca cgtcatacgc atctgcttca taatgttcca
     6901 atcgtttgtt cctcctttta ttttcctaaa caaaattgac tgaataattg agtgattagc
     6961 tcatcttgta cactgtcgcc gacaacttct cctagatagt cccagcaacg cgtcatatct
     7021 atttgaacga gatccacagg cattcctgaa tcaatacctg caatgacttc ttcaagtgag
     7081 agggaagctt tttctaacaa cgcgatatgg cgggtattag aaacataagt agcgtctctt
     7141 tctcctgttt ctccgctgaa gaataaatca gaaatagctg attccaaacg atctagtccg
     7201 tcgtttttag ctacggaaat tgaaaaaact ggttcatttt cgatcaattt cttcaatttt
     7261 tcttgttcca gctgtgctgg caaatctgtt ttatttagaa gaatgatccg tttcaatcca
     7321 cttgtcgctt ccaataattg ttcatcttct gctgtcagtg gttcgctttg gtttagaaca
     7381 agtaagatca agtctgcttc tgctaatgct tttcggcttc tttcgacccc gatccgttcc
     7441 accacatctt ctgtttcccg aattccagct gtatcgatta gcttgagcgg taccccccga
     7501 acattgacat actcttcgat tacatctcga gtcgttcctg caatatccgt aacgattgct
     7561 ttttcttctc ttaacaaatg gtttaacaaa cttgatttgc cgacatttgg ccggccgatg
     7621 attgccgtac ttaacccttc ccgcagaacc ttcccttgct tggatgtagc aagtagcgct
     7681 tggattcttt gttggatcat ctgcgctttt tcaagcaata acttcgtagt caattcttcg
     7741 acatcatcat attctggata atcaatattt acttctactt gtgctaatgt ctctaaaatc
     7801 tcttgtctca atgagcggat caaagcagac agattgccgt ctaactgatt aagagctagt
     7861 cccattgctt tgtctgtttt tgcccgaatc aaatccatca cagcttctgc ctgtgaaagg
     7921 tcaacacgac cattcaagaa tgctcgtttt gtgaattcgc ctggctcagc taatcttgca
     7981 ccttctctta acaatagctg caaaatttga ttgactacaa cgatcccacc gtgacaattg
     8041 atttctacta catcttcccg cgtaaatgtc tttggtgctc tcatcacgga gaccatgact
     8101 tcatcgacaa gctgattgct cttgggatca acgatatgtc cataatggat cgtatgactt
     8161 ggtacttctg ataatcgttt gtttccagat tgataaactt tatctgctag ttgcacagct
     8221 tgatcgccac ttaaacgaac gatactgatt gcaccttctc caggtggggt agaaattgcc
     8281 gcaatcgtat caaattctaa agtaatttca gccatttttt cacttccttt ttcattttgc
     8341 gcacaaaaaa agtgcccact ccacccttta tgcacaataa acggtgaact tcgcactttg
     8401 actgcttgtt cttgattaaa ttctggtcat agattatcac acttgctata aggaatcaag
     8461 tgtttaaaac aattagatga agttgagcct gttctgcttc cattacctta tatttaattg
     8521 tccttggatg tctctttgcg ttctttggtt tattcgaaga gaagatactg tataaacaag
     8581 gctttatctt tcttaaaaaa aacggtacac taaatggtag cgctatctaa actatacact
     8641 tttgaaagga gaaaatatgt gagcccgttc agatctgctg cattggaaga agaaagtgac
     8701 cctcggatca acgatgtcgt tttatctaca gggaatatca accagaaata tatcgtccgc
     8761 gatatcgttt ttgttgtgga atgcttagaa gctgatttgt ttgacccatc ttttcccaaa
     8821 gatgaccttt tcttaacaac gaagaggaaa ttaaagacga aggtacttga atatggtgcg
     8881 aatgcagtaa tcaactgtca ttttgagtat cagcaaaaaa aacgcgacgg aaagtatttt
     8941 ctggaacttt ttgcttatgg aacagtgatc caatttatcc aaacaacgat tggttaaatg
     9001 aaacagacaa aaaaagcgga tcaaaactga agacagtttc gatccgcttt tttctatcaa
     9061 agttagtgac tacttcatcg tatactctga taagaatgat ttctagtaaa atatacttgg
     9121 tttgaaaaac tttgtcaacg tgatttattc aaaaagtaat ggaatcaata atatttttta
     9181 gcaggttcta ctactaaata acgataaggt tcctcacctt ccgaatgtgt ttttacatgt
     9241 tcatctttgc ttaatgcagc atggatttgt ttacgttcaa atgcaggcat tggttctaag
     9301 aaaacaggct gccctgtccg ttttactttt tcagccgtgc gatcagctaa acgctgaatg
     9361 atctcttggc gcttttcacg gtaattgcct acattgacta ctacagacag cttatttctt
     9421 gctaaacgat ggatgaagac ttgtgccaga tattgcaatg cattcagtgt ttttccatgt
     9481 ttcccaatca gtatgccttg cttttgtgtt tctaaatgga aaacaaccat attatttgat
     9541 tctctttcca ttttgacaag agctggagca tcaagctgtt ttgaaatttc agtaagataa
     9601 actgccaatt ccgttaaagc tgcttcatca cctagctcac ttacttcttc tgttttttct
     9661 tcaagaatcg tttcgttgac tacctcgatc tcagctgttg gttcttcttc caaaatttct
     9721 tctacagtct gttctactgt ttcagaaact ttttcgctga tacttggctg gatcgaaagc
     9781 cgtgcattct ttcgacccat tcctaggaaa cctttttttc cttcttccag tatttcgatt
     9841 tcgacttggt ctttggccaa acccaaatca gccagaccta gttggacagc ttcgtctatt
     9901 gttgcagctt cataaatcgg cattagcatt acctccccta ttatttccgt tttttcttag
     9961 gattcattgc tttcttaaga gcacgttctc tttcgcggat tctccgtgct tcttcttcac
    10021 gctcttttcg aattttgaaa gggttgttga tcagcagcgt ttgtacaact tggaaggcat
    10081 tggaaactac ccagtataat gaaagaccgc tggctaaatt gacacccatc actaaaatca
    10141 ttacaggcat cacgaaattc atgatcttca ttgatgcatt cgattcaatt tgactcatgc
    10201 tagataaata cgtacttgca aaagtaaaga ttgctgctaa aatcggcaaa ataaatgttg
    10261 gatccgcgtt ccctaagttc aaccataaga aatgcccttg tttcaactct ggtacacgtg
    10321 aaatagattg ccataacgcc atcaagattg gcatttgtac taataaagga agacaaccag
    10381 catatggatt gacgccattt tctgcatata aacgctgctg ctcttctctt aacttacttt
    10441 gtgtttccgg atctcttgaa ctatattgtt gctgcagtgc ttttaatttc ggttgtaatt
    10501 cttgcgtttt acgcatgctc tttgtttgga aatgcatcaa cggcaatagg atcacgcgga
    10561 tgatcagtgt aaataaaatg atccctatcc ctgcattacc aaatgacaac gccttgatcg
    10621 cctcagcaaa gtaataaaca atataacgat cccaaattcc tgtactctgt gcgctgactt
    10681 ccccagttcc gcaaccggat aagacgatga ccaaactaac gataccggcc atcaaaagta
    10741 aacgtttgta tttcttcact tactcttttc ctctctgtcg attatttttg caagttttaa
    10801 gacgtggact aaattggagc gaacctcatc ataagtcaac cccttcacac ttggtctagc
    10861 aatcacaata aaatccaact ccggatcaat ttcttctttt atagaataaa ctgctgctcg
    10921 gatttttcgt ttgacttcat tgcggtttac cgcgttgccg accttttttc ctacagataa
    10981 accaactcga aaatgaggct gtcccggttt ttccaaacgg taaacgacga atttgcggtt
    11041 agcaaaagaa tctcccgtat ggaacacggc ttgaaactct cgttcttttt ttactcgata
    11101 cgcttttctc attagttttc tttccttttc tatctctcac ttacgctcat aatcatacca
    11161 taattacgat tgtcctttca tcatttccct aaaaaagagt ataaaaaaaa ccactgaaac
    11221 cccagtggcc taagctgaaa gaacttttct tcctttgcga cgacggctag ctaacacacg
    11281 acgtccattt ttagtactca tacgtttacg gaatccgtga actttttgac gtttacgttt
    11341 atttggttgg tatgttcttt tcatatattc cacctccaaa atgttcttat ctattttata
    11401 aacacagaca tactttgata gtataacgac aaagtagtga ttttgtcaat ataaaataga
    11461 agatcgattg ttaaaactta tccacaagat tatttttttc tatgggaatc ctatgtggaa
    11521 aaagttatgc acagggatta tccacagata ataaaaggct ggttttacac agaaaacaca
    11581 cgtgtaaaaa aagtaatcca caatcaggcg atttcctgtg tattccgtct cctaactatt
    11641 ggtatatctg tattttttat ccacagacgc aagaggaaat ctattggttt ttacgagttt
    11701 tccacctgtg cattagtggt gtggaaaact ttttttgaca actgtgtttt tctctttttt
    11761 ctttttgtcg attgtgtgga taattgtaat taaaaaattt ttttcttcgg tttctgtgga
    11821 aaactattga aaaaaatgct aaattagtac tagttattca ctttttaatg aaggaggagg
    11881 ccttatatgg tatccctcga tgctttatgg aatgaattaa aagcaacata ccaaaaggat
    11941 ttatcgcctg ctagctataa tacatggatc gaaacagcga accctcgaac ccttgatcaa
    12001 aaccagctcg ttgttgaggt tcctagcaag attcataaag aatattggga aaaaaacctg
    12061 gcgacaaaaa tcgttgaagt cggttatatg ttatcaggaa atgagattat ccctcgcttt
    12121 attactggcg aagaagcaga gcaagaagaa gttatagaag aaaaaaatcc aaaagttgtg
    12181 gcacctagtc cgctaaaaaa agccatgctg aaccctaaat atacctttga tacgtttgtc
    12241 attggtaaag gaaaccagat ggctcatgca gcagcgctcg ttgtagcaga agatccaggt
    12301 tctatttata acccgctctt cttttatgga ggcgtgggac ttggtaagac tcacttgatg
    12361 catgcaattg gtcatcaaat gctgcaaagt cagcctaatg ccaaagtaaa atatgtaagc
    12421 agtgaaactt ttgctaatga tttcatcaat tcgatccaaa acaaaacagc agaagagttc
    12481 cgtcaagaat atcgaaatgt tgatttactg ctagtggatg acattcaatt ttttgcagaa
    12541 aaagaagcta cgcaagaaga gtttttccat actttcaatg ccttgtacaa tgaagggaaa
    12601 caaatcgtac taaccagtga tcggttgcca aacgagattc cgaaactgca agaacgcctt
    12661 gtttctcgct ttgcatgggg gctgtctgtt gatattacac cgccggatct tgaaacaaga
    12721 acagcgattt tacgtaaaaa agcggcggct gaacgtctag agatcccaga tgacacacta
    12781 agctacatcg caggacaaat tgattctaac attcgtgaat tagaaggtgc gcttgttcga
    12841 gtgcaagctt ttgctgcgat gaatagtgaa gatatttcga ctagtctagc tgcagacgca
    12901 ctgaaaacgt taaaatcagg aaaaggacat cctcaattgt caatcctgca gatccaagaa
    12961 gaagtggcaa agtattatca catccaattg aaagatctca aaggaaagaa acgcgtcaaa
    13021 tctattgttg ttcctagaca aatcgctatg tatttggcac gtgagctcac agataattct
    13081 ttaccgaaaa tcggagctga attcggtggc aaggatcata caacggttat ccatgctcat
    13141 gaaaaaatcc agcagctgat ggattctagt gtcagtatgc aaaatgaagt atcagaaatc
    13201 aaaaactttt tactaaatta atttgtcgat tctatcaact gaaaatagca tgtggataaa
    13261 agaaaaaaac gttcaaaagt tatacacact ttttccacag ctgttttttc gtttctctct
    13321 tacgtttttt gagttttcca cagaatcaac atgccctatt acttttatta ttctttttaa
    13381 ctaataataa ataaacaaac attcaaagga gatacatcat gaaagttact ttaaaccgag
    13441 ctagctttat gcaggaattg caaactgttc aacgagctat ttcaagcaaa accacgatcc
    13501 ctattttgac aggtgtaaaa atcacactga cacaagaagg tttgactttg acggggagca
    13561 acgctgatat atcaattgaa acttttttgt ctgttgaaaa cgaaaaagca aatatgcaaa
    13621 tcgaatctac tggttccatt gttttacaag cacgtttctt tagcgaaatc attcggagac
    13681 ttcctgaaga aacatttact ttagaagttt tagaaaataa acaagtagcg atcacttctg
    13741 gaaaagcgaa ttttatcgta aatggattag atgcagataa ctatcctcat cttcctgttg
    13801 tcgaaagcca taaccagatg aaattacctg tacacgtatt gactaaacta atcaacgaaa
    13861 cagtttttgc tgtctcacaa catgagagtc gtccaatctt gacaggtgtg catttcattt
    13921 tatctgataa ttctttatta gctgtagcta ctgattctca ccgtctaagt caacgcgtga
    13981 ttccagtaga acaagcggct gatcattttg atattgttat tcctggaaaa agtttgatcg
    14041 aattatctcg ctcattaaca aatgaagaag aaatcgtcga aatcagcatt atggaaaacc
    14101 aagtgttgtt caaaacagaa acgatgtatt tctattcccg tttgctagaa ggaaactatc
    14161 cagataccaa tcgtttgatt ccttcaagtt ttaatacaga ggttgaattt tctgttccta
    14221 gctttttagc ggcgatcgaa cgggcttctt tactttctca tgaaggccgt aacaacatcg
    14281 ttcgtttatc tattcgtcca gatgcggttg ttttatacgg aaattcgcca gaaatcggga
    14341 aagtagaaga aagcttgagt tatacagcaa gcagtggcga tccattagat atttctttca
    14401 atccggatta tatgaaagca gcgttgcgtg catttggaga tatgagcatc aaagtgaaat
    14461 ttatctctgc tattcgtcca tttacattgg aaccaacgga agatggcgtt caatttattc
    14521 agctgattac tcctgtacgt acaaattaac aatgattttc aatgttcttg aaaatttaat
    14581 tgaaaagaaa tagaaaaaat aaaattttaa acagaaggcc ctaaaatctc ttttaaggct
    14641 ttctgtttat ttttgtttaa atcacctttt ttagattaaa tcgtcttata tggaaaaata
    14701 tatggataaa tttgttttat tgcgaaaaaa aaggtataat agagtatact tttaataatg
    14761 aaaatgaggg atgggcattg aaacaacaag tcattctaaa tacggaattc atgacgttgg
    14821 gccaaatgtt gaaagaagtc acagttatcg gtagtggcgg acaagcaaaa tggtatttgg
    14881 cagaaaacac tgtgttagtc gatggcgaac cagaaaatcg tcgtggacgc aaactttatc
    14941 ccggcatgat ggttgaagtt ccagaagtag gaactttttt tatggtaaaa aaaggaaacg
    15001 acgataatga ggctgaataa gctgtattta aaaaattatc gaaattacga agagctgaac
    15061 atcgaatttt ccaagaacct cgtcatcttt ttaggagaaa atgcgcaagg aaaaacgaat
    15121 cttttggaaa gtatctacgt attagctatg acgcgaagtc atcggacaag tagtgagcag
    15181 gaattgattg gctggactga cgatcaggcg atgattcaag gagagatcac aaaaggttca
    15241 tccactcttc cattagaaat cttattatct aaaaaaggcc gaaaaaccaa gatcaaccat
    15301 atcgaacaga aaaagttaag tacttacgtg ggacaaatga atgtcatttt atttgcacca
    15361 gaagatcttt ctttagtaaa aggcagcccg cagatcaggc ggaagttttt agatatggaa
    15421 ttaggccaga tcaatccaat ctatctttat gatcttgtcc aataccaagc aattttgaaa
    15481 caaagaaacc agtacttgaa gcaattgaat gaaaaaaaac aaactgattt gctttatttg
    15541 gatattttgt ccgaacaact agcagcgttt ggcagcaaag tattgaaagc aagagcacag
    15601 ttcgtcaagc gattggagtt ttgggctaat tctttgcatc agcaaatcac ccatcaaaaa
    15661 gagcagctgg aaatcgagta tctcaccgca gtcgattctt tagaaacaca tacgcaagag
    15721 caaatccaag aacaatttct cgcacttttg aaccaaaata aaaagaaaga cctttttcgg
    15781 ggaacaacga cagttggtcc tcatcgtgac gatctttcct tttttattaa tcagaaaaat
    15841 gtgcagactt atggatcgca aggacagcaa agaacaactg cactaagtgt caaattagca
    15901 gaaatcgatt tgattaaaga agagacaggg gaatatccaa tccttttgtt agatgatgtg
    15961 atgagcgagc tggatgataa tcgtcaactc catctgctag aaacaatcga aggaaaagtt
    16021 caaacgttct taacaacaac gacacttgac catgtaaaaa ataaaatgac tgttgaacct
    16081 gagatttttt atgttcaaca agggaaacta aaaggagaag aaactgaatg acagaagaaa
    16141 gaagtttagt agaacgcgct aaagagtatg atgccagtca gattcaagta cttgaaggac
    16201 ttgaagccgt tcgtaaacga cctggcatgt atatcggttc gactagttct gagggtctgc
    16261 atcaccttgt ttgggaaatt gtcgataatt cgatcgatga agtattagca ggttttgcaa
    16321 cgaaaatcca tgtgattatt gaaaaagaca acagtatcac cgttattgac gacggtcggg
    16381 ggatacctgt agatatccag gcaaaaactg gacgtcctgc agttgaaacg gtctttactg
    16441 ttttacatgc tggaggaaaa ttcggcggtg gtgggtacaa agtttctggt ggacttcacg
    16501 gagtaggatc ttctgttgta aacgcgcttt ctacacagct tgatgtcaaa gtatacaaag
    16561 aaggaaatgt gtactatcaa gaatatcgtc gtggagctgt agtggacgac ttaaagatta
    16621 tcgaacaaac ggatcgccat ggaacaacgg ttcactttac gccagaccct gaaattttta
    16681 cagagacaac tgagtttgat ttttcaaaat tggctacccg gatccgtgaa cttgctttct
    16741 tgaaccgcgg gatgcgtata tcgattgaag acaaacgaga agaagaacca gtcatcaatg
    16801 aataccatta tgacggcggg atcaaaagtt atgtcgaata cttgaatgca aacaaaaccg
    16861 tgatttttcc agaacctgta tatttggaag gtgaacagca agatatcgca gttgaagtct
    16921 ctatgcaata tacagacggc taccattcca atatcatgag ttttgccaac aatatccata
    16981 cgtatgaagg tggaacacat gaatcaggat tcaagactgc tttaactcgt gtgataaatg
    17041 attatgcccg aaaacaaaag ctgatgaaag aaaatgacga caacttgacc ggtgaagacg
    17101 tgcgagaagg attgacagct gttatttcta tcaagcatcc tgaccctcaa ttcgaaggac
    17161 aaacaaaaac aaaattgggc aattccgaag tacggacagt cactgatcgg ttatttagcg
    17221 aacatttcat gaaattcttg ctggaaaacc caagtgtggg ccggcaaatt gttgaaaaag
    17281 gattactagc ttccagagct cgtctagcag cgaaacgtgc gcgtgaagta acgagacgta
    17341 aaggtgcttt agagattagt aatctgccag gaaaattagc ggactgctct agcaacgatc
    17401 ctgaaaaatg cgaattgttc atcgtcgaag gagattcggc cggcggatca gctaagcaag
    17461 gtcgtaatcg agagtttcaa gccatcttgc ctattcgtgg gaaaatcttg aatgttgaaa
    17521 aagcaagcat ggacaagatc ttggcaaatg aagagatccg gtctttattt actgctatgg
    17581 gtacagggtt tggagaagat tttgatgtat cgaaagctcg ctaccacaaa ttagttatca
    17641 tgactgatgc cgatgtcgac ggcgcgcata tccgtacatt gctgctgact cttttttatc
    17701 gttacatgcg tcctattgtt gaagctggat acgtctatat tgctcagcca cctttatacg
    17761 gggtaaaaca agggaagaat attacgtatg tacaaccagg aaaaaatgca gaagaagagt
    17821 tgaaacaagt cgttgcttca cttcctgcct caccaaaacc aagcgttcag cgttataaag
    17881 gacttggaga aatggatgat catcaactat gggaaacaac tatggaccca agcaatcgga
    17941 tgatggcccg cgtcagtgtg gacgatgcta ttgcagcaga tcagatattt gaaatgttga
    18001 tgggggatcg agtagaaccc cgtcgtgcat tcatcgagga aaatgcacac tatgtgaaga
    18061 acttagatat ttaatcatga aggaggaaaa agaatgagtg aagaaatcag agaaaacatc
    18121 catgatgtca atctaaccag cgaaatgaaa gactccttca tcgattatgc gatgagtgtt
    18181 attgtcgcac gtgcacttcc tgatgtcaga gatggtctga aacccgttca tcgccgtatt
    18241 ttatacggca tgaacgaatt aggtgtaaca ccggataaag cccataaaaa atctgccaga
    18301 atcgtcggag atgtcatggg taaataccat cctcatggag acattgcgat ctatgagtca
    18361 atggtacgga tggcacagcc attcagctac cgttatatgc tagtggatgg tcatggaaac
    18421 tttggttctg ttgacggtga cggtgccgcc gctatgcgtt ataccgaagc acggatgagc
    18481 aaaatcgcta cagaaatgct gcgtgatatc aataagaaca ctgttgattt ccaaagcaac
    18541 tatgatgata cagaaaaaga accagttgta ctacctgctc gcttcccgaa tcttttagtg
    18601 aacggaacaa cggggatcgc cgtaggtatg gccacgaata tcccacctca taacttgtct
    18661 gaggtagtag cggctatcga tttgctgatg gataatcctg atgtgacgac caatgaattg
    18721 atggaagtat tacctggacc agattttcca acaggtggtt tggttatggg gaaatcagga
    18781 atccgaagag cgtatgaaac aggtaagggt tctattaccg tacgtgcaaa agttgagatt
    18841 actgagatgc caaatggtaa agaacggatc atcgtgacag aattgcctta tatggtcaat
    18901 aaagcaaaat tgatcgaacg gatttctgaa ctgcacagag acaagcgtat cgaagggatc
    18961 acagatttgc gggacgaatc ttcccgtgaa ggtatgcgga tcgtcattga catccgtcgt
    19021 gacgccagcg catccgttat cttgaataac ttatataaat tgacatcctt gcaaaattca
    19081 tttggattca atatgttggc gatcgaaaaa ggtgtgccga aagttttgag cttgaaacaa
    19141 atccttgaaa actatatcga acatcaacgc gaagtcatca cacgacggac ggtctttgaa
    19201 aaagaaaaag cagaagcaag agcacacatc ttagaaggtt tgcgtattgc tttggatcat
    19261 atcgatgaaa tcattgcgat tatccgcggg tctaaatcag atgacgaggc aaaagcaaca
    19321 atgatcgaac ggttcgattt atccgatcgc cagtctcaag ctattttaga tatgcgttta
    19381 cgccgattga ccggtttgga acgggaaaaa atcgaaaatg aataccaaga actcttaaaa
    19441 ctgattgaag atttaactga cattctggca agaccagaac gtgttacaga aatcatcaag
    19501 aatgaattag cagacttgaa tcaacgattt ggtgataaac gacgcacaga attactagtc
    19561 ggtgaggtct taagtctgga agatgaagat ttaatcgaag aagaagaaat cgttattaca
    19621 ttgacaaaca atggttacat caaacgtttg gcaaataatg aattcagagc tcaacgaagg
    19681 ggtggacgtg gtgtccaagg aatgggtatc cacaatgacg acttcgtcaa aaatctggtt
    19741 tcttgttcta cccatgatac attgttattc tttacgaata acggaaaagt gtatcgggca
    19801 aaaggatacg aaattccaga atacgggcgt acagccaaag gaatcccaat catcaattta
    19861 ctaggtatcg attcgagtga aagcatccag gcaatcattg cggtgacggg agaagcagaa
    19921 gaaggccact atctcttctt tacgacgcgt caaggtacag taaaacgtac ttctgtcact
    19981 gcttttgcta acatccgaag taatggtctg atcgcaattg gtttgaaaga agacgatgag
    20041 ctcgtcaatg tgctactgac agatggtcaa tctaatatca ttattggaac acataacggc
    20101 tactctgtca ctttcaaaga agaagcagtc cgcgatatgg gacgtacagc ttccggggtc
    20161 agagggatac gcctgcgtga ggaagattat gtcgttgggg cagccttaat gaaggaaaat
    20221 caagaagtat tagtgatcac agaaaaaggt tacggaaaac ggacaaaagt atcggaatat
    20281 ccagtgaaag gccgcggagg taaagggatc aaaacggcga acatcactga aaaaaatggt
    20341 ccactagctg gattgaccac tgtttctgga gatgaagaca tcatgctgat tacagataaa
    20401 ggtgtcatta tccgtttcaa cgtatcgact gtttctcaaa ctggacgttc aacattaggc
    20461 gtacgtttga tgaagatgga agctgatacg aaagttgtca cgatggcagc agtcgaacca
    20521 gaagctgcag aacaaacagt tgaagaacaa ggatcagaag aataaccatc aagactaagt
    20581 gtgtaagcta gcaagaataa ccgattaaaa aatctggtta ttcctgctgg cttttttgat
    20641 tagttgaatt tctcttaaaa tattcgaaac tgttcattct gtattgcttt tgaccaaaat
    20701 agagtgtata attagagctt gtgagtagtc taattaatta gataactctc cttgctctta
    20761 ggaacctaag agccgaaagt ccacagggag gtgaatatca atggaaaaca cgaaatatga
    20821 aatcatgtac attattcgtc caaacattga tgaagaagca aaaactgctt taatcgaacg
    20881 tttcgatgca atcttgaaag ataacggagc agaagttatc gaatcaaaag attgggaaaa
    20941 acgccgtttc gcttatgaaa tgaacggtta ccgtgaaggt atctatcaca tcgttaaagt
    21001 atcttctcca tcatcagcaa atgctgtcaa tgaatttgac cgtcttgcta aaatcaacga
    21061 cgatatcatt cgtcacatga ttgttaaaga agaagcttaa ttgttgtttc atgtgaaaca
    21121 atagaaaacg atgagaggag atgactattt tgattaataa tgttgtatta gttggacgtc
    21181 tgacgaaaga tccagatttg cgttacacag caagtggtac ggcagtcgca acatttactt
    21241 tggctgtgaa tcgtaatttt acaaaccaaa gcggcaatcg cgaggctgat tttatcaact
    21301 gtgtgatttg gcgcaaatca gcagaaactt tagcaaacta tgctcgtaaa ggaacacttt
    21361 taggtgttac tggacgtatc caaactcgtt cttatgataa tcagcaagga caacgtgtat
    21421 acgtgacaga agtagtagct gacaacttcc aattgctgga aagtcgttct gcatctgaaa
    21481 atcgccaaca aagcggcgga ttccaaagtt ctggtcaatc agctggcgga tttggcggaa
    21541 acaataactc taatcaaaca tctcaatcat ccaacggtat gcctgatttc gaccgtgata
    21601 cttctgaccc attcggctca agttcaacga tcgacatttc agacgatgat ttaccattct
    21661 aaacgaattt gataggaggg aaatagaatg gcacaacaaa gaagaggcgg acgtaaacgt
    21721 cgtaaagtag actacattgc tgctaaccat attgaatata tcgattataa agatatcgaa
    21781 ttactaaaaa gatttatttc agaacgcggt aaaatcttgc cacgtcgtgt aactggtaca
    21841 ggcgctaaaa accaacgtaa aattactgtt gcaatcaaac gcgctcgtat catgggcttg
    21901 ctacctttcg ttagcgaaga ctaataaatc ctaggaattg aatgactgat ccttttgatc
    21961 agctattcaa ttcctttttt tctgtaaaaa taacctctat tcgtttagtc gctcgaatga
    22021 cgaaagttat ggtaaaatca aattagtcat atatgggagg aaaattatgg aaaataaaaa
    22081 caatcattta tccccgaaac ctctcttcct tatcctgttt atattaatcg aagtattggt
    22141 agtcttaatt ttaccatttc gttttgttag cgcttcggtc attctattga tgaatttatg
    22201 gggtgttcgc tattggttga aaatgaaacg ttggcttatt ttaaataaac gtgaaaaaat
    22261 ccagcaggct tcgactgtag ctgaaaccaa tctagcttat gtctcagaag tcatgcctgt
    22321 aggagtgatt gcttatcagc catcaagcca aaaaatcgaa tggctcaatc cattagcatt
    22381 gaaaatcaaa gatcaaagtg gattaacaga ggaagaattg ctagatactt tttttactgc
    22441 aaaagaaaaa aagaaaaacc aagtctatct agcagataaa acatttcagt ttgatatgga
    22501 tacagtaaag ggtgttgttt attttaatga tattacccaa aaccagttgt taaaactcca
    22561 tcagacgaac agtcagccgg tgattggtat tttatctatt gataactatg cagatgccat
    22621 tgataaatta gatgacaaag agatttctta tttgaactct ttcgtcacta cactagtttc
    22681 tgactggatg aatgaatacc atgtcttttt caaaagaatc aacgcagaac ggtttttctt
    22741 cgtagcacag atgatcgatc tgaaaaagat gatggataaa aaatttgatt tgctggatcg
    22801 ctttcgagca gaagctgaaa aacagagctc gcctatcacc cttagtatgg ggattgctta
    22861 tggctcaaga accctcgatg aaatcggtgg agaagctcag aataatttag atgttgcttt
    22921 agtccgtgga ggcgaccaag ttgtcgtgaa agaggtaaaa gaaggagcaa aacctgttta
    22981 ttatgggggg aaatctgcta agaatgtcaa acgcacgcgt gttcgttctc gagcgatgag
    23041 taccgctttg cgtcggattt ttgatgaaac aggcgatgtg tttatcatgg ggcacaagtt
    23101 tccggatatg gatgctttag gctctgcttt tggtgtcagt tgtctggcac gcttcaacca
    23161 gaaaaaagct tacatcgtta tcaatgagaa cgagatcatt ccagatgtag aacggtgtct
    23221 tgaagaaatc cataagcatc ctgaattaga aatgcaacta atctctccag aacgtgccat
    23281 agagctgaaa aaagaagagg acctcctcgt gatggtggat taccataaac cttctctatc
    23341 gatctctcag ggcctatatg agcaatttga taagattgtg atcattgatc accatcgtcg
    23401 tggggaagag tttccaagta aacctctgct gacttatatt gaatcttcag cttcttcagc
    23461 ttcagagcta gtttctgagc tgatccaata ccaaagcagc aaaacaaatc gcttagatcg
    23521 ttttgaggcc acattattgc ttgcagggat ggttgtagat acaaatagtt tcaatgtccg
    23581 tacaactgca cgaacttttg acgttgccag ctatttaagg tcttgtggag cagacgcatc
    23641 cttagtacaa tatttattga gctcggattt agcgacatac ttggagatca gcaatttgat
    23701 tgctggaagc gactatgtga cttctgatat tgttgtggcg gcagggacag acgaaaaaga
    23761 gtataatagt gttatagcag caaaaacagc agatacattg ctttcgatga tcaatatcaa
    23821 tgcagctttc gtcattacaa agcggacaga cggcttgatt gggatcagtg ccagaagtac
    23881 cggcacgatc aacgtccaaa tcatcatgga atccttaggt ggcggaggac acttcacaaa
    23941 cgcagctact caactggaaa atacgacagt tgaggaaaca aaacagaagc tatttgctgc
    24001 aatcaaagaa aatatgaatc aaatatatga taaggagtaa tccaaatgaa agtaattttt
    24061 ttacaagatg taaaaggaaa aggtaaaaaa ggggacatca aagaagttcc agcaggttat
    24121 gcacaaaatt atctaatcaa aaataacttg gcaaaagaag caacgaaaag cagtatcagt
    24181 cagttagcag gacaaaagaa agcacaagaa aaacacgaag ctgaaatctt ggaagaagct
    24241 aaaaagttaa aagaattctt ggaaaaagaa gatactgtag tcgaattgaa ggcgaaagca
    24301 ggggaagaca gccgtttgtt tggttcgatc ccttcaaaac aaattgctga agggttaaac
    24361 aaacaataca aaatcaaatt agataaacga aaaatcgaat tagaaaatcc gatccgttca
    24421 ctaggctaca caaacgtacc tgttaagctt catcatgagg tgacggctac gatcaaagtt
    24481 catgtcgtac aagaataaca aatcttgata gcgatgttca gctggtaagt ataaccagaa
    24541 ggaaaaaaga caaaccgaaa tttatctgag aaaacagctg cagcaatgaa agcaaaagct
    24601 gcaaatcgat ttcagattga tttcggtttc ttttttggga acagcaaaat agcataaaga
    24661 aaacgagtat aataaaagag aactattttt ttattacact ttcggtacga atgatgactt
    24721 ttctctcatt ttcttgtaaa atgaagcaat tgaagacaag gagcgatacg tatgaatgaa
    24781 gtgtggcagg atcgaatccc accgcaaaat attgaagctg aacaagcggt tttaggctct
    24841 gtttttcttg acgcagaaac gatcattgat gccatggagt acatagagcc aaaagacttt
    24901 tatcgtcgtg gccatcaaat tatttttgag acaatgatcg agttaaatga tcgtaatgaa
    24961 gcgattgacg tggttactgt caaagaccgg ctggaacaag ccaatttgtt agaagatgcc
    25021 ggtggtttaa gttatctctc agacttggca ttagcagttc cgacagcagc aaatatcgtc
    25081 tactatgcga aaatcgttga agaaaaatca ttgctgcgta cattgatcca gacagcaaca
    25141 ggaatcgtta cgaaaggatt cgagcaagga gaagaagttc aaacaatttt agatgatgcc
    25201 gaacgcagta tcttggaggt ttcggaaaaa agaaatcgaa gcggcttttt atctattgct
    25261 gaagtactga actccacgat tgaaaatatc gatcgactag ctcaaaacaa tgaagaaatc
    25321 acaggtctac caactggcta tcaaggtctg gataaaatga cagcaggttt ccagcccgaa
    25381 gaactaatca ttcttgctgc acgtccagcg gtaggtaaaa cagcgtttgc attgaatatt
    25441 gctcaaaatg taggaacaaa aacagatcgt tctgttgcga tatttagttt ggaaatgggg
    25501 gcagaatctc ttgtaaatcg gatgctttgt gcagaaggtt cgattgaggc cagccattta
    25561 cgtacaggtc agctttcgga agaagaatgg tcgaatctga tcgttgcgat ggggagtctt
    25621 tctcgagcaa atatttttat cgatgataca ccagggatca aaatctctga aatccgtgcg
    25681 aaatgtcgga aactagcgca agaaaaaggc aatctaggct tgatattgat cgattatttg
    25741 cagttgattg aaggtaacgg taaagagaac cgtcaacaag aagtatcaga tatctctcgc
    25801 caactgaaaa aactggcgaa agagctgaaa gtacctgtca ttgctctttc acagttatct
    25861 cgtggagtag aacagcgaca agacaaacga ccagtgctaa gtgatattcg tgaatccgga
    25921 tcaattgaac aagatgcgga catcgtagcc ttcttgtatc gagatgatta ttatgaaaga
    25981 gaaggcggag atgacgatga tggaaacgag ccaaaaaatg acaacgtcat cgaagtcatc
    26041 atcgagaaga accgaagcgg tgcacgagga acagttgaac tactgtttat caaagaatac
    26101 aataagtttt cgtcgctttc gccaagagaa gaattttaaa gaagaggttg tgagccttga
    26161 gcaatcccga gagctctcgt aagaagatgc aaggagtaac cgcaggaacg actagagtac
    26221 ttaatttaaa cagatctcca aagggattgt gacaaaagtc ttgtcacagt ccctttttcc
    26281 gaataaacgg tgttcaatca gctgaccctt ggtattagct cagcaaacgt caaaatatga
    26341 ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatc ttacgacctc
    26401 ttttccttaa acggtgttct cctgccaact cctcggtcac aagtcacaat atcatcaaac
    26461 atgagaagca gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa
    26521 cctctttctt aaacggtgtt caaaagcttg catctgcggt aatacgccca atcaaacgaa
    26581 aaatatgaga agctattttt gtttggctgt tcttattact tgatgcagga cagctttttc
    26641 acaacctctg attcacggtg ttccaaaagt agcttcttcg aaaaataagt cgaaatttca
    26701 caaaaatttg agaagcaatt ttcgtacatt ccttcttatt tcttgaagct gaccacttct
    26761 gttacaacct cttttttaat aaccattcta aaaagataaa gttcgttttt taagcgtttt
    26821 tataaaataa aaataaaaat cattcgattt tatttgaaat atgtatctat tattgataca
    26881 atggttcagg attaaataat atagaacgag gtgttcacat gtcatctgta gtagtagtag
    26941 gtacgcaatg gggcgatgaa gggaaaggaa aaatcacaga ctttctcagt gaaaatgctg
    27001 aagtgattgc acggtaccaa ggtggcgaca atgcagggca cacaattaag tttaacggtg
    27061 tgacttataa actgcatttg attccttctg ggatttttta taaagacaag atcagtgtga
    27121 tcggaaacgg cgttgtagtc aatcctaaat cattggttaa agaattagct tatttacatg
    27181 aaaaaggtgt agaaacaagt aatttgcgta tttctgaccg cgcgcacgta atcttgcctt
    27241 atcatatcaa attggaccaa ttacaagaag atgctaaggg cgaaaataag attggtacaa
    27301 cgattaaagg aatcggacct gcctatatgg ataaagcagc tcgtgtaggt atccgtattg
    27361 cagacttatt agataaagaa atttttgaag aacgtcttcg tttgaatcta gatgacaaaa
    27421 accgtcaatt tgtaaaaatg tttgattcag aaccacttga ttttgacgat atttttgaag
    27481 aatattatga atacggacaa caaatcaaac aatacgtcac agatacttcc gttatcttga
    27541 atgatgcttt ggatgcaggc aaacgcgtgt tgtttgaagg cgcacaaggt gtaatgctgg
    27601 atatcgacca gggaacttat ccttttgtta cttcttctaa cccagtagcc ggtggggtaa
    27661 caatcggaag cggtgtaggg ccttcaaaaa tcaataaagt tgtcggcgtc tgcaaagcct
    27721 atacgtcacg tgtcggtgat ggaccatttc caactgaatt atttgatgaa acaggagaac
    27781 aaatccgcga agtaggacga gaatacggta cgacaactgg tcgtccgcgt cgtgtaggct
    27841 ggtttgattc tgtagttatg cgccattcta aacgagtttc aggtattacc aacctatctt
    27901 taaactcgat cgatgtatta agtggattgg aaacagtaaa aatttgtacg gcttatgaat
    27961 tagatggcga actaatctac cactatccag caagcttgaa agagttgaac cgctgcaaac
    28021 caatctatga agaattgcca ggctggtcag aagacattac aggatgcaaa acacttgcag
    28081 aactgccaga aaacgcacgt aactatgtac gtcgtatctc agagcttgtt ggtgtacgta
    28141 tctcaacatt ttctgtcgga cctgatagaa cacaaacaaa catcttagaa agcgtctggg
    28201 cgcaaatcta aggataaaaa cgacgactga atcaggacgt tcttaccaga gcgagtcgaa
    28261 gtgtttatct atacgataat aataacttga agaggtagtg aaagagacgt ttagccttat
    28321 tgccgatagg ctgtctcttg aacactgttt attcagaata agggactgtg acaaaaaccg
    28381 tgtcacagtc cctttttccg aacaaacggt gttcaaaagc ttgcatctac ggcactaagc
    28441 ccaatcagat gaaaaatatg gagtgctatt tttatctgat tgttcttact gcttgatgca
    28501 gagcagcttt ttcacaacct ctccactaaa cggtgttctc ctgccaactc ctcggtcaca
    28561 agtcacaata ttcaacaaac atgagaagct gttttttgaa attgttcctt gtgactcgga
    28621 gctaaacggc aggctcacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
    28681 tagctcaaaa aacggcaaaa tatgaggaac tattttttcc gtttttttct ctaatactca
    28741 ggtcaaaacg ctgatttcac aacctctgat tcacggtgtt ccagaagtag cttctacgga
    28801 aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct
    28861 tgaagctgac cacttctgtc acaacctctt attttgatta gaacttgcga aaataaaccg
    28921 gttaaagtgt gtctagcgca agagatttgc tataatagac gataagggta aaatgtaacg
    28981 agaaggggtt gaaaaatgtg tatcaaattg taacagattc ttgttgtgat ctgccatacc
    29041 atgtattagc aaaagaaaaa gttgattttt tatcaatgaa tctccaattg aatgatcaag
    29101 agttagtaga tgacctcgga aagacattca attatgaccg ctacatgaat cagctgaaaa
    29161 atggtgcgat gcctacgaca tcacaaatca atgttggccg atatttagaa tttttccgtt
    29221 cttatgcgga aaaacagatt cctgttttat atgtggcttt ttccagtgga ttaagcggct
    29281 cttacagtag tgcgcttcaa gcggttgaat tgctgaagga agaatatgaa gaccctgaga
    29341 tctatgtttt tgatacaaaa gctgcaagta ttggggaagg gcttttagta ttggaagctg
    29401 taagattgaa aaaagaaggt aaaagcatcg aagaaaccct agagtggctg gaaagtaatt
    29461 atttgaaagt ccattcatgg gtaacagtag acgatctgaa atatctagaa cgaggcggta
    29521 ggatttccaa agcaagtgct actcttggca gtctattgag tgtaaagcct attattacgg
    29581 tggataaaga aggaaggctg acgaatgttg acaaagttcg aggaagaaac aagtcgatcc
    29641 agacaatcgt caatgagacg gtaaaaacaa tcgttgcacc acttgatcaa atcgtttatg
    29701 tggcttacgc tggggatcta gagagcgcgg agaaggcaaa acggcttttg gaagagcaga
    29761 taaagatgaa agatgtgaag ctttatccct taggaccaac gatcgccagt cacacaggct
    29821 atggctgtat cgcgattttt tcgatgggaa tcagtcgata aaagcagtaa ctcattctgt
    29881 aaaaaagccc agaaattgat tgtaacaaaa acagcgactc ttaaaagata gatgagcatc
    29941 ctatctctta gaggtcgctg tttttttatt ttttaagcta aataggccat tatgcccata
    30001 actcgttcaa ttgtttttga atatctgcat tttccaaaaa ttcatcatat gtcgtatcag
    30061 ctcgatcgat cacaccttta tccgaaacgg caatgatgcg gtttgccaat gtttggataa
    30121 attgatggtc atgtgacgca aataagattg agccagtaaa tgccatcaag ccgtcattca
    30181 atgctgtgat tgattctaag tctaaatggt tcgttggatc atctaataca agtacgttag
    30241 ctttcgaaag catcattttt gaaagcatgc aacggacttt ctctccacca gataaaacat
    30301 tcactggttt caatacttct tcaccagaaa acaacattct tcctaagaag cttcttaaga
    30361 aggtgttatc atcttcttct ttacttgcat attgacgcaa ccagtctaaa atcgttagat
    30421 cagtatcgaa ttctttcgtt gtatcctttg gtaaataaga attacttgtt gtcacacccc
    30481 aacgtactgt tccagtatct ggcgtaattt cacccatgat aactttgaat agcgttgttg
    30541 ttgttatatc attttctgcg acgaaagcga ccttgtcttc tttgctcaaa ttaaaagata
    30601 tatcatctaa aattttcttt ccatcaatcg ttacacttac attttctacc tgcaataagt
    30661 cgtttccaat ctcacgttca ggtttgaatc caacgaatgg ataacgtcta gaagaaggtt
    30721 ggatatcgtc taaagtaatt ttttcaagca tttttttacg tgaagttgct tgttttgatt
    30781 ttgatgcatt cgcactaaag cgggcaataa attcttggag ttctttgatt tgttcttctt
    30841 ttttggcatt tgcgtttgct tgaagtttag ccgctagttg actagattcc aaccaaaaat
    30901 cataatttcc tacataaagt ttgattttac caaaatctag atccgccata tgtgtacata
    30961 ctttgttcaa aaagtgacgg tcatgggata caacgatcac agtattttca aaattgatca
    31021 aaaattcttc cagccaattg atcgattgga tatccagacc gtttgttggt tcgtctagca
    31081 aaagaacatc aggtttacca aatagtgttt gtgctaataa cacttttacc ttttgtccac
    31141 cagttaattc actcatttgc tgatggtgca attcttcagg gatgttcaga ccttgcagta
    31201 aaacagctgc ttctggttca gcttcccatc cattaagctc agcaaattct ccttctaatt
    31261 cagctgctcg aataccatct tcgtcagtga aatcctcttt catgtaaatc gcatcttttt
    31321 ctttcagcac ttcatacaga cgcttgtgtc ccatgatgac tgtttcaagt acagtatagt
    31381 cttcatagtc gtaatggttc tgtttcaata cggccatccg ttcatctggt cccatagtaa
    31441 cggaacctgt ggaaggctgc agatcccctg ataacacttt aaggaacgtt gatttacctg
    31501 caccatttgc accaatcaag ccatagcagt ttccgggggt aaatttaata ttcacatcat
    31561 caaatagttt gcggtctgaa aagtgcaaac tcacatcatt tacagtaatc aatttttttc
    31621 ctcactttta tcattattag ttttgacaag tcttatagaa ttatataggg gaaaggtaaa
    31681 agattcaagg caatcgaaca ttctttgtgt ttcacgtgaa acagaatgaa aaaaataaaa
    31741 ataaaagcgg agcgaaaata gctactatag gatttattag tttgcaacag cataattttt
    31801 gacgttattt ataagaggga gtactataaa actgtaaaag aaatgaaaga aggattgaaa
    31861 atggatgaaa aaagaaccat cgactggggc tcattgattt tgggaatttt atttgtactt
    31921 gtctcattgt tatcatttca agaccctgtc ggaaatttag ttgcaattgt agtagtcttt
    31981 gcgatttttg cctttattaa agggatattt gaactgtttg tacggaacag attaaaagag
    32041 ctaacaggat ataaaggaaa gatgccctta ataatcggga tcatcgatat tctagttggc
    32101 gtctttttct tattcaatat tggtgcagga gtcgcagcat tgccttttgt ttttgcagta
    32161 tggtttattg ctgattcagt tttagcgtta tttactgctg acttggcaag aggagtaagt
    32221 gaaggatatt attggtttac gatcatcgtc aatattttag gaattttatt aggaattttc
    32281 ttattgttta atccaatttc ttctgcgttg acattaagct ttttagttgg attctatttt
    32341 atgttatttg gtatcacaca tatcgtctat gcatttagat aagtaaaatc aaacaaataa
    32401 aaaagccggc ttccgattac agttttactg ttgtaattgg aagccggctt ttttatcatg
    32461 gacaaatatg cacgaggtga tttttaggtg tcgagacaaa tttatgcaaa aatgtgagaa
    32521 gctatataca caaattttat cttaatactc gaagcatagc gcagttgtca caacctcttt
    32581 tcatataatt cattcaatac tggcaaatca tggacttcat atttttcttt gaatgcttgg
    32641 atttcttctt cagaatattt tcttggatct aaatgcagat cttctactgg caatgggcgc
    32701 ttgttattga tcttcacatc gattactaca ggacgatctg attttgcagc tgcatcaaag
    32761 gcaaatcgaa gttgcgagta ttctgtgact gtaaagctat ttgccccgag cgcttctcca
    32821 actttaccga aatctgcatc ttctaaaaat acaccaaatt tcttttgttc cgtatcttct
    32881 tgttctgctt cgatgaaacc aaaagaatca ttcgaaaaga caacattaat aattggaagc
    32941 tgatatttga cttgtgtaat gatatcttgc atgaccatag caaacccgcc atcaccactt
    33001 aaggtaaata cttgtttttc tgggtagctt agttgtgcgg caattcctcc tggcacacca
    33061 ttacccatag ttgcgaacca tccagatgtc gtatgttttt gtttaccgtt catattcaat
    33121 aaacgaatag aatggatagt cgtgttaccg acatctgtga cgaaaatcgc atcgtcttca
    33181 gcaatacgat tgatttcttt atagacagcc tctggacgaa tcggttcttc ctctcgatct
    33241 tcaaaagatt tcaaccaatt tacccatttt tctttatttt cttgatttgc taggtaccag
    33301 ctatcagcag ggcgagcatc ccctaactcg acaagtcttt gcaaagctgt gtttgcatca
    33361 ccgaggatcg acaaagatgt ttcatggcgt cgtccaaatt ttgacgcatc gatatcgatt
    33421 tggataaatt cagcagttgg attaaaaaat gcacgtccaa atgggaagtc gctaccgaca
    33481 aataagatca gatcggcttc tgccaatgct tcatttccgg gtttagtagc gacacgtcca
    33541 gcaaaaccaa gaaagttttc ataatcatca ggaataattc cttttgctag aacggaagcg
    33601 acaacaggaa ctgaaaaatg ttctgagaat tctttgattt gcggaaatcc gtttcgcgtt
    33661 ccttgaccaa cataaagaac tggttttttt gctttttcaa gataaggcaa agcagctagc
    33721 aaatcttttt cttctggtaa tatcacactt gttttgtgag tgtgagcggt ggagaaaggt
    33781 tgttcgtcaa tttcttcaaa accaagatca acagggatag tcacgatagc cacgccttta
    33841 tgctcatagg cggctttaat ggcttcatct actacatgcg gaagactttg tggtgtcatg
    33901 actgtacgat tgtatacgct aacatctgcg aaaataggat tttcatttag ttcctggaaa
    33961 gagttatagt tcatggatgt agaagcaact tgtcctaaga acgctactag aggcacatgg
    34021 tccatctgtg catcatacaa accattgatc aaatgtgtcg caccaggtcc tgcagagccg
    34081 aagacagcgc cgactttccc cgttaatttt gcatctgcag cggctgcgag tgcgccgact
    34141 tcttcatgac gaacttgaat gtagttgatt tcggtttttt cttgatatag cgcatccata
    34201 attgaattga aagatcctcc gggaatgccg taaatatggt cgatacccca gctttcaaga
    34261 acttttacca ttgcgattcc tgcattgatt tttgacatgg tgtttcactc ctttagcaat
    34321 gatgatacat aaattttttg tatctagagt atagaacttt ttatcgaaaa tcccaactaa
    34381 ttcgtctttt cacaaaaatg tagtcatgtt tttgggaata tgctagagta gtacatagat
    34441 agattcaaga gaggggaatg gcatggttga atggctattg agacttgaac cttggcagca
    34501 agcacttgct gggacagctt ttacttattt tatgacagct cttggtgccg ggttggtttt
    34561 tttctttaaa gaaatcaaaa aagaagtgct aaacttgatg ttaggatttg cttctggtgt
    34621 catgattgct gcaagttttt ggtctttgtt agatccggct attacaaaag cagaagaaaa
    34681 tggagatatt gcttggctag ttgtaagtat tgggtttggt ttaggcggtg tgtttttata
    34741 tatggcagat aaaacactgc cccatatgca ttttggacca caacatgaag cagaaggatt
    34801 gcctacccat ttaaaacgga cgatcttgct tgttttttct attacattgc ataacattcc
    34861 tgaaggttta gccgtggggg tagcattcgg tgcagcagca actgcggata atccgacagc
    34921 cgctatatta gccgctgttt cggtggcgct aggtattggt atccaaaact tcccagaagg
    34981 agcagctgta tcaatcccgt tgcgacaaga aggtctgagt cgtaaaaagg cctttgttta
    35041 cggacaagct tctggaatcg ttgaaccaat tgctggagtg attggagcgc tactagtaac
    35101 gaaagtggag ctgctgcttc cttatgcact tgcttttgcc gcaggagcaa tgatttatgt
    35161 cgtggtggaa gaattgatcc ctgaagcaca gcagacattg tccagcaaac gtcattttgc
    35221 agtttttggc gtcatgagtg gatttatcat tatgatgatt ttggatgtgg cattaggata
    35281 aataaaaaaa atatatatga tatatcaaat agggccgcga acttatatcg cgaccctatt
    35341 tttttagtaa gttatatcat aatgaagtga atggtatcat acattaataa tagttttttc
    35401 tatgtgtatt tcctttgatc cagcgaacaa gccagatgat aagtgcgatt gggattagaa
    35461 ctttgaccgc aaaccataaa agcccgctta ggacactgac aaaaagactg atgacgaagc
    35521 tagctgcaat tagcactaaa agaaccatga agatatttaa gaacatattt ttttgacgca
    35581 tacaaaaccc tcctcttgtt cattctttac tatagaaaaa gtataacagg gattgtttaa
    35641 aaacacctcc aactgaagac tgatttttac ttcatctact taaatgatca aacaattgct
    35701 ttttctctgg tttatcatct aaaatagagc ggaaaaggag gagaatatca tgcctaaaaa
    35761 tcgtcttgaa gcatttacag atgctgtaat cgcgattatc atgactattt tagttttaga
    35821 acttcatgaa cctaacggag acacgtggga ggcgctcgct gcattaggtt accgattttt
    35881 tatctatatt gtcagtttta tcattttagc gatttattgg aacaatcatc atcatttatt
    35941 tcaagttgta cataagataa atggacgggt cttatgggcg aacagtttct ttgtttttgc
    36001 gctttcgctt tttccgtttg caacttcatg gatcagtgag tacatcaata gcctggctcc
    36061 agaaatgact tatggttttg ttaccttgac cgctaatatt gcgtattata tcttggcaaa
    36121 agaacttgtc aaagcaaata aaggccacga gaaagcattg aaggcgatgt tgaataatta
    36181 caagaaatcg tatctatcga ttggtttgaa tgtgttagga attgtcttag ggtatttagt
    36241 agcgccagta gccgtgctga tcgcaaacat cgtgattcta gctgcttgga tcatcccaag
    36301 tcgtaccatc gaatcacaat atcggtaaaa tgatccgtgt ttaaaattta agagaaaaat
    36361 atagaaaaaa taaataaacg atcaaatatc cgagtgatac tagtattcaa agagggatgc
    36421 agatggatca aaaaagaatt catgtaggac tgattactag ttttgtcacg atgcttattt
    36481 atttggatca aggtcatttg acttggacag tagttggtgt cgtaggattt ttgacattat
    36541 tcacagcaat aaaaaaataa acatctttat gtaatctgat gttctatcta tttaagaaaa
    36601 gcatgtttac agcagtatga ttctgttgta aacgtgtttt tatttgtgga aaatttaatt
    36661 tttagaaaag tgtatcgaaa aatagatagt tagtttttcc acagtggata acttcgtaag
    36721 agataaaaat aaaaaattag tcaaagaagg gcgta
//
LOCUS       DADXTG010000015        34507 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.015, whole genome shotgun sequence.
ACCESSION   DADXTG010000015 DADXTG010000000
VERSION     DADXTG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.015"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..2066)
                     /locus_tag="IWI57_000610"
     CDS             complement(24..2066)
                     /locus_tag="IWI57_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448776.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            complement(2056..2850)
                     /locus_tag="IWI57_000611"
     CDS             complement(2056..2850)
                     /locus_tag="IWI57_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448777.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            3149..5338
                     /gene="nrdD"
                     /locus_tag="IWI57_000612"
     CDS             3149..5338
                     /gene="nrdD"
                     /locus_tag="IWI57_000612"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBC2448778.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            5653..6255
                     /gene="nrdG"
                     /locus_tag="IWI57_000613"
     CDS             5653..6255
                     /gene="nrdG"
                     /locus_tag="IWI57_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBC2448779.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(6309..7430)
                     /gene="dinB"
                     /locus_tag="IWI57_000614"
     CDS             complement(6309..7430)
                     /gene="dinB"
                     /locus_tag="IWI57_000614"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBC2448780.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(7539..8411)
                     /gene="rsmI"
                     /locus_tag="IWI57_000615"
     CDS             complement(7539..8411)
                     /gene="rsmI"
                     /locus_tag="IWI57_000615"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBC2448781.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            complement(8480..8827)
                     /locus_tag="IWI57_000616"
     CDS             complement(8480..8827)
                     /locus_tag="IWI57_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBC2448782.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            complement(8820..9644)
                     /locus_tag="IWI57_000617"
     CDS             complement(8820..9644)
                     /locus_tag="IWI57_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBC2448783.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            complement(9680..10618)
                     /gene="holB"
                     /locus_tag="IWI57_000618"
     CDS             complement(9680..10618)
                     /gene="holB"
                     /locus_tag="IWI57_000618"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBC2448784.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            complement(10632..10961)
                     /locus_tag="IWI57_000619"
     CDS             complement(10632..10961)
                     /locus_tag="IWI57_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBC2448785.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            complement(10976..11620)
                     /locus_tag="IWI57_000620"
     CDS             complement(10976..11620)
                     /locus_tag="IWI57_000620"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBC2448786.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            complement(11684..12280)
                     /gene="recR"
                     /locus_tag="IWI57_000621"
     CDS             complement(11684..12280)
                     /gene="recR"
                     /locus_tag="IWI57_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBC2448787.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            complement(12305..12619)
                     /locus_tag="IWI57_000622"
     CDS             complement(12305..12619)
                     /locus_tag="IWI57_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBC2448788.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            complement(12639..14384)
                     /gene="dnaX"
                     /locus_tag="IWI57_000623"
     CDS             complement(12639..14384)
                     /gene="dnaX"
                     /locus_tag="IWI57_000623"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBC2448789.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            14599..15846
                     /locus_tag="IWI57_000624"
     CDS             14599..15846
                     /locus_tag="IWI57_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBC2448790.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15871..17055
                     /locus_tag="IWI57_000625"
     CDS             15871..17055
                     /locus_tag="IWI57_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBC2448791.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            17052..17849
                     /locus_tag="IWI57_000626"
     CDS             17052..17849
                     /locus_tag="IWI57_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBC2448792.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(18291..18986)
                     /locus_tag="IWI57_000627"
     CDS             complement(18291..18986)
                     /locus_tag="IWI57_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBC2448793.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            19140..19418
                     /locus_tag="IWI57_000628"
     CDS             19140..19418
                     /locus_tag="IWI57_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448794.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            19437..20645
                     /locus_tag="IWI57_000629"
     CDS             19437..20645
                     /locus_tag="IWI57_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2448795.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(20793..21158)
                     /locus_tag="IWI57_000630"
     CDS             complement(20793..21158)
                     /locus_tag="IWI57_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBC2448796.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(21305..22078)
                     /locus_tag="IWI57_000631"
     CDS             complement(21305..22078)
                     /locus_tag="IWI57_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2448797.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(22252..23112)
                     /locus_tag="IWI57_000632"
     CDS             complement(22252..23112)
                     /locus_tag="IWI57_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBC2448798.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(23112..24530)
                     /locus_tag="IWI57_000633"
     CDS             complement(23112..24530)
                     /locus_tag="IWI57_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2448799.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(24587..25036)
                     /locus_tag="IWI57_000634"
     CDS             complement(24587..25036)
                     /locus_tag="IWI57_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2448800.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(25481..25744)
                     /locus_tag="IWI57_000635"
     CDS             complement(25481..25744)
                     /locus_tag="IWI57_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBC2448801.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(25843..27138)
                     /gene="rho"
                     /locus_tag="IWI57_000636"
     CDS             complement(25843..27138)
                     /gene="rho"
                     /locus_tag="IWI57_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBC2448802.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(27135..28406)
                     /locus_tag="IWI57_000637"
     CDS             complement(27135..28406)
                     /locus_tag="IWI57_000637"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBC2448803.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(28571..29440)
                     /locus_tag="IWI57_000638"
     CDS             complement(28571..29440)
                     /locus_tag="IWI57_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBC2448804.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(29732..31342)
                     /locus_tag="IWI57_000639"
     CDS             complement(29732..31342)
                     /locus_tag="IWI57_000639"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBC2448805.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(32536..33876)
                     /gene="glnA"
                     /locus_tag="IWI57_000640"
     CDS             complement(32536..33876)
                     /gene="glnA"
                     /locus_tag="IWI57_000640"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBC2448806.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(33904..34296)
                     /locus_tag="IWI57_000641"
     CDS             complement(33904..34296)
                     /locus_tag="IWI57_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2448807.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT        10048 a         7248 c         6120 g        11091 t
ORIGIN      
        1 attatcttgt cttattccgt atatcattcc tcgataatcc gacaatataa ctgactcgta
       61 atgacataaa gcaaaagata gataaggatc aataagccac tcacaagata agccaaccag
      121 taatcggcag tttttatata ctgagtgaag acattgattg caaatactgc gtgagtgatc
      181 cctaatatca tgggtggaaa gaaggtaagt gcgttttgct cgaagatcat ccgtctgatc
      241 cttctttgtg gaatccccag cttagtcaat aggtgataat tttctttttc ttcctctgct
      301 tctgctaatt gccggactgt caaaatacta ccagtagcaa taatcacgat catcccgaca
      361 aatagtgtga caaaaatatt gataccgatt cgccgattaa ctgcacgcaa tttcgaatag
      421 ctggaagaga aattcaaacg agagctttct tggctaaatt gtgcttcctt catttggtca
      481 tccggaagtt tctttgtgct aatttctcct tgtagacttc catttacgat atcataagaa
      541 taataaacag cattgagcca ctttatcgac aggtcattgc tcagtcgttc atctattttt
      601 tcattatttc cacctgatac atctacattg actacttgat aatccacacc ttttgctttt
      661 tcaaaaacgg tatcatctac aatcagtaca ggacttagat ataccattgt ttcgtcacct
      721 aatacacttg gaagcatttg ttgaatcttc aattcttgat tatctggcag tccaatacta
      781 gatccatata atgaaatatt ccgaaaaaga gtttgcattt gatcaagcaa aacagtatga
      841 ccaggtgatt tcaaagaaat gttcggcaaa tctggattaa cttttctgaa ggcttgatat
      901 tgtgtttccg tgatcagatt agcagtttga aacataggtt cttcgagatt ttgactatag
      961 atactcccaa tgactttata atgcaaggtg atttctttag ttatcttctg cccttcctct
     1021 gctagaatcg gcttgatttt tttctctgtc tcaacatcaa actgatagtc catcggggag
     1081 gaaactttct cctggtagga taaaatagta gagatactag ccgtgccgcc aattgtcatt
     1141 aaagaaattc cgagaatgac tgtaatcaga gaatttgtac gccaactttt gagcaaatga
     1201 acttgcgtgt tacccattat caaaaaattg ataccatagt aataaatctt ccatttactc
     1261 aatatgatca aaaataaccg aaatgtataa cagaaaaaca aataagtacc gaccacacaa
     1321 acgaaaaaaa ttaaaaaagg aaaaaagaaa actaatgtat caagataatg ataactgatc
     1381 atccatgaag tgatttccct aaaatgacta gctccaaaat atccgctcgt taaaagtagt
     1441 aacccgatga ctgcgagtaa tcggtgcctt gtcctgaagc gcattgttga actatctgct
     1501 tgttcttttt ctccaaaact tcgaatcatt ggatagcgcc atattttcca tattgaatat
     1561 acagatactg ctaaaaggat gatgaaaaaa gcaaataaag tatctactac tgatggaatg
     1621 gagataaaaa atggactagt aagatccata tccatcattc gaaccaaaat cattgaaaag
     1681 agtttggaaa aaataatacc gagtaatatc ccagaaatac aggcgaacaa tccaattatc
     1741 atgatttcta acacatatat acctgatatc tgcagtttat tcataccaaa aagctgatag
     1801 atgcctattt ccttttgcct tctattcaag aaaaatcggt tggcactgaa tacgaaaaaa
     1861 agcaaaacaa cagtaatgat aaatccacca aaactcaatc caccatctat cgaagtatct
     1921 tgacttgctc ttctgactaa agattgatcg taggtcattg cgctgaagga ataatagatc
     1981 attacggata aagtcaaact gaaaaaataa acaagatagc ctcgtgactg ccgaaaaaaa
     2041 cttttaacgg ctagtttata cagcattcat ttttcctcct ccgccaatcg tcgcctgcat
     2101 gtctattact ttctcaaaga actctcttct tgtccctctt ctgacaactt cagaaaaaat
     2161 cacaccatct ttaataaata aaattctatc gcagtagctt gctgtatatg gatcatgggt
     2221 gactaacagt atcgttgctt tttctttttg attcatggtc ttcaagtagt tcaacaattc
     2281 tgtagccgac ttcgagtcca aagagccagt tggttcatct gcaaaaatca cttgtggctg
     2341 aacaacaagt gctctagcag ctgcaattcg ttgtctctgt ccgacagaaa gttcatcggg
     2401 ataccgtttg agcagttctt ctatattcaa caattgagct acatgtctta ctctcttttc
     2461 catttcctct ttagtcatcc gttcaactgc taggggcaat aggatattgt cttttgcatt
     2521 tagggtatcg atcaaattga actcttgaaa aatgaatcct agtttttttc gacgaaagtc
     2581 actgagctca ctgtttttca tttttgtgat ttcttttcct tggatcgaga cactgcccat
     2641 cgtaggcaaa ttgatcgtgg acaagatatt catcagcgtt gtctttcctg caccacttgg
     2701 gcccataatc ccaacaaact cgccttcttc tactgtgaat gagatattat ctaaggcttt
     2761 gacgggagaa agtttgctgc cgtagctttt acttagatgt ttcacttcga tcatcttttc
     2821 caattcttct cctcctcttt catttttcat ttctatttat ttgcatagta gcatatctac
     2881 gaataatttg ctcttatgtt tttgtcactt ttacttggac tgaataaaaa attttattca
     2941 attattttat tcagcaaaat taaatatgca aaaaacaaat ccaccagcta caagttttca
     3001 ccttttttca caataaaata tacactacat atagtataga aatccaatta tacagcacat
     3061 tgaatactat atatagccga tatttagttg ctccttcccc gattttgttt tatgatagac
     3121 aagaacaatt tagcaggagt tgaagataat gaaagtaaat tatgaagaaa aaactgtcgt
     3181 tactcagcag ccagagacat cccagatgaa agtcatcaaa cgcgatggcc gccatgttga
     3241 tttcgatgaa caaaagatca atgaagcact gatcaaagca gaaaagaaaa ttcacggctc
     3301 attatcacca cttacttatg aaaaaattca aatgattgca gacttggttg tccaagaaat
     3361 caaaagccgg tttgctgaaa atgtcaaaat ctatgagatt caaaacatcg tggaacatat
     3421 attactagaa aaaaacgaat atgaattagc agaagagtac atcaattatc gtaccaaacg
     3481 agattttgct cgcagtaaag caacagatat caacttttcg attgaaaagc taatcaacaa
     3541 ggaccaaact gtcgtgaacg aaaacgcaaa taaagacagt aatgttttca atacacagcg
     3601 agatctgaca gccgggatcg ttggaaaatc aatcggactg aaaatgttgc caccacatat
     3661 agcaaatgcg catcaaaaag gcgatatcca ttaccatgat ttggattatc atccttatac
     3721 accgatgacg aattgctgct tgattgattt caaaggcatg ctgaacaatg ggttcaaaat
     3781 cggcaatgcc gaagtcgaat cgccaaaatc tatccagacc gccacagcac aaatctcaca
     3841 gattattgcc aatgtagcgt caagtcaata tggtggctgt tctgccgacc gaacagatga
     3901 attgcttgct ccttttgcag aattgaacta taaaaaacat ctaaaagacg cggaagaatg
     3961 gatcgattcg ccagaacgcc aaaaagaata tgcaaaagca aaaaccaaaa aagatatctt
     4021 cgatgctatg caaagtttgg aatatgaaat caatacctta tttacttcaa atggacaaac
     4081 tcctttcact tcattaggat ttggactagg agaaaactgg tttgaaagag aaattcaaaa
     4141 agcaatttta cagattcgaa tcaatggatt agggagtgaa aagcggacag ctatcttccc
     4201 taaactgatc ttcactttga aaaaaggtgt gaacttaaac cctgaagatc ctaattacga
     4261 catcaaacaa ctagcattgg aatgtgcaac gaaaagaatg tatcctgata ttctgaatta
     4321 cgataaaatc gttgaattaa ctggaagttt caaagtacct atgggctgtc gttcattctt
     4381 gcaagggtgg aaagacgaaa atggtcaaga agtaaatgtc ggacgaatga atttaggtgt
     4441 tgttacattg aatttgccaa gaatcgcgat cgaatcaaaa ggagatcaga ataaattctg
     4501 gcagttactg tctgaccgct tagaaatcat gaaagatgcg cttctctatc gtgtagaacg
     4561 ttgtaaagaa gccattccag caaatgcgcc aattttatat atgtatggtg ctttcgggaa
     4621 acgtctttca cgtaccgatt ctgtcaatga attattcaaa aaccgtcgag ctactgtatc
     4681 gttaggctac attggattgt atgaagttgc ttcagctttc ttcggtggcg aatgggaaac
     4741 aaacccagaa gcaaaagctt tcacacttga tatcgtcaaa gaattaaaag ctaatgcaga
     4801 tgcatggggg gatgaatatg gttatcattt cagtgtctat tcaactccaa gtgaaagtct
     4861 aactgaccgt ttctgccgtt tggatacaga aaaattcggc atcatcgaaa atatcacaga
     4921 taaagaatat tatacaaaca gtttccacta tgatgtgcgt aaaaacccaa cgccatttga
     4981 aaaattggaa tttgagaagg attatccaaa atattgttca ggtggattca tccactactg
     5041 tgaataccct atgctccaac aaaatccgaa agctttagaa gctgtatggg attttgcata
     5101 tgacaaaatc gggtatcttg gtacgaatac gccaatcgat cattgctatc aatgtggttt
     5161 cgaaggtgat ttcaaaccga cagaacgagg gttcgaatgt ccagaatgtg gaaatcatga
     5221 tccaaaaaca tgtgatgtag tcaaacgtac ttgtggctat ttaggaaatc cgcaagctcg
     5281 tccgatggta catggccgcc ataaagaaat ttcctcacgt gtgaaacaca tgaaatgagg
     5341 ttgtgaaaaa gctgtcctgc atcaagtaat aagaatagct aaacaaaaat agttcctcat
     5401 atttttcgtt tagttgggct tattactgca gatgcaagct tttgaacacc gtttattcag
     5461 aataagaggc tatgacaagt cttttttcac caccctcttt cgatggcttt gtatctttga
     5521 acgccctcta tcaaaataag aacaagcttt cttgaacaat ggatttaggt cagtttgttc
     5581 ttatttatgt ggtcgagtag attttatctt caatagctat atctttgaac cttaaatgaa
     5641 agaagttttt ttatgagaaa tccaaaacca aaagaatggt tagcaaaaga ctacagccaa
     5701 aactatattg ctgactacaa accgtttaat tttgtcgatg gtgaaggtgt ccgcaacagt
     5761 ctttacgtta gtggctgctt atttgcctgc gaaggctgct tcaacaaagc tgcacaaaat
     5821 ttccgatatg gcaaaccatt cacacctgaa ttagaagaac aaatcatcgc tgacttgcgc
     5881 aacgattacg tgcaaggatt aactttactt ggtggcgaac cttttttgaa tacagatgtt
     5941 tgtctgcaag tcgtacgacg tatccgaaaa gagttcggta cgacaaaaga tatctggtca
     6001 tggacaggct atacctttga tgaattgcta caagattcag aagataaatt agaactcctt
     6061 tcgcaaatcg atatccttgt tgacggacgc tttgaattaa gcaaacgtga cctaaaacta
     6121 caatttcgcg gaagtagcaa ccagcggatc attgacgtcc aaaaatcttt agaatccaat
     6181 caagttgtga tttgggaaaa atgtacggat gcaacagaaa cctatgaaca aatcaaaaag
     6241 caagacttga tataaaaaca gctttgggag agaaaacgga tttccggttt caactctcaa
     6301 agctgttttt atatttcttg attttcccat aatggaagga cgatgttttc ataagtcatc
     6361 gggtccagat tcgtcaaagt aatccctaac aatcggattc ctttttctac tcccaagatt
     6421 tcctcccata taaggcttgc ttgataaaag agtgcttctt ttttatagat atattctggc
     6481 aatgtcactc ttttggtcac agtagaatag tccgtgtacc tgaccttcaa aactactgtt
     6541 ttcccatgtt tttggactct tctcagtgct tcttctactt tttcagctag ttggcgcaac
     6601 tgagtcagca ctgcttcttc cgtttgcaac ggctgcccat aagtatgctc ttttcccaca
     6661 gattttcgct ctcttgtaac gtttactgga gaatcatgga ttcctctgac tttacgataa
     6721 agagaatagc ccatttttcc aaaatttcga atcaacatca tttctgtgca ttcatatagg
     6781 tcttttcctg tataaatccc tagctcatgc attctaggta cagttttctt ccctacccca
     6841 tgaaattttt caattggcaa agcttttaaa aatggcacag cgtcttgagg ggcaactacc
     6901 gtgattcctc taggtttttg atagtctgat gctaatttgg ccaaaaactt attgtaactt
     6961 actcccgcag aacatgttag ctggacactc tcccaaatat ctgcttggat cattcgggcg
     7021 attttgatgg ctgacttgca attgatttta ttttcggtca catcaagata cgcttcatcg
     7081 atactaactg gttccacaat atctgtataa cgatagaata cttcccgtac ttgtttggaa
     7141 atatcgctgt atttatgatg gtcgccaggc ttaaagatgg cttgcggaca aagctcatac
     7201 gctttttgtg cactcattgc tgaatgaatt ccgaattttc ttgcttcata atttgctgta
     7261 gtcactactc cacgcccacc agtgtcactg gggtgtctgg caattactaa tggatgtccg
     7321 cgaaactcag gatgatctcg ttcttcaacc gaagcgaaaa aagcatccat atcaatatga
     7381 atgatttttc ttgatgtatc attatgtaaa ggaaattgca gctccatcat cctcacctct
     7441 tattcattat acaagaacat tcgttcgcgt tcaagagatt tgaaaagtta gtagctgatt
     7501 tcctcattag tctttcatta tagacaactt atactatctc agtctaattc atggtactga
     7561 cggtagactt cttgtttttt cagcccattt ttcagcgcta ctgctttgat agcagcattt
     7621 ggtttttcgc ctgaagcgat ctttgcttct acttgttcct ttaacgttcc caactcttca
     7681 tcaacttccg tcactggcat ttcgaacaaa ttcccttcta caagcagaca acattcaccc
     7741 tttactggat gttcttcgat ataagcgagt agctcttccg tacttcctct aaggtattct
     7801 tcatggacct ttgtcaattc ccgacacaaa acgacttggc gatttccaaa aatttctaag
     7861 aaattcgtca atgtagcagt gatccgataa ggtgactcat aaaaaatcag cgtaactgtc
     7921 tgttctttta gctttttcaa tgtttctttc tgttcttttt tcttacgtgg aaggaagcca
     7981 taaaataaaa atggttgagg cactagccct gaagcaatca aagccgtcat cccagctgta
     8041 ggtcctggga tagagatcac agaaatccct tcttttatgc aggctaaaac tagctcatgt
     8101 ccaggatcgc tgatggaagg cattcctgcg tcactgactt gagcaatcgt ttttccttct
     8161 tttaacaaag caatcaattg agggacacgt tctttataat tatgttcatg taaacttttt
     8221 tggggtgtat ggatttcaaa atgattcaat aacttttgtg tattacgcgt atcctcactt
     8281 gcgataagat cggattcttg cagcatacga acacttctgt agctcatatc ttctaaattt
     8341 ccaattggcg tcggtacaag atataaatcc ccatataccg gatttccctt gaaacttttt
     8401 tgattataca tctgtcacaa tctccttaat aaaaagactg gagcagtatt tctccgctcc
     8461 agtctcctct tgttattggt catcgaccgc tttcgcgata gatcacttct agacaaaacg
     8521 cacaaggctc gtcattgtcc cttcttgatc catataaatc gtagcagaca tggaatcctt
     8581 cttcataaat tttttcaaga ttcatacgag acttagaaag ttcctgcttc acattttcag
     8641 ctggagcttg tgcaacttgg ttcaattccc gcaggtgttc ccgcagacgt tgattttcca
     8701 tctccaaagt tgtatttttt tctacgatct catgcaaagc atgcttcatt tcaacaagtt
     8761 ctgttaatgt atttctcaac tctgattcta attgattcaa tccgtcgtat aatgatctct
     8821 tatccattca tctcacctac ttggataaat tctttcaatt catcccattc atattcgaca
     8881 ggattttccc gtccagtcag ccgaatacta acgatacggc taagcagatt caaccctacg
     8941 actttccctt taccatcagg tgtagcaatt acttttccaa agtcagggag ttcttttttc
     9001 gctgtctcgt attcttcgct ctcatattgc agacagcaca tcaagcgtcc gcataaaccg
     9061 gaaatttttg ttggattcaa agataaactt tgatcttttg ccattttgat tgatacaggc
     9121 acgaaatcgc ctaaaaaaga agaacagcat aatggacgcc cacatggacc aattccgcct
     9181 attatctttg cttcatcgcg gacaccaatt tgtcgaagct caatacgagt acgaaataca
     9241 gcggccagat cttttaccaa ttctctaaag tcaattcgcc cgtccgctgt gaaagaaaaa
     9301 atcaatttat ttcgatcaaa tgtgtattcg gctttgacca ttttcatttc caactcacgc
     9361 aactgcactt tttcttttgc gattttcaat gcatcataag catctttttt gttttttttg
     9421 tctttttcta attcagcttg tgacgcttta tgcaagatcg gacggatagc ttcaggaagg
     9481 tcattgggat caatctcttt tttaggaagg acaacagtag caatatgtaa ggattgctga
     9541 gattcgacta ataccttgtc actataaaca tattcagagt ctcctggtgc aaaataatag
     9601 atatgacctg catctctata tcggacacct actacttcta ccatttccat cctccttgat
     9661 catcatcctt tgcggcaagc taaaaaatcg tatgcaaaac gaactgttcc gcaacagcct
     9721 gaaaactgac attgctgcgt agtttctgct ctgcgatcaa tatccgttca attatctggt
     9781 ttatctgttt cactttgcca tattcgccgt ttgctactgc ttgacgctgt gcttcctgat
     9841 aataaaacat caagacagat aatcctgtaa actgttgtgt tttttcttta aatgatttta
     9901 ccaatttttt ttggacatag ataaaggctt gagtatcttg cttttgcaag taaacgaacc
     9961 attgataaat caagtcctta gcatcattaa accattcatc ttgagagatt tcaactgctt
    10021 ttcctaaact gtttgttaag aaagccaaaa gttttgcttt ttccaatgga atctgctctt
    10081 cttgcaatcg atggatcagt gcttctttcg aaagttcttg aaaatgcaga acttgacaac
    10141 gagattgaat agtagggaga attcttccta ctgcatctgt ttctaacacc gctaaaaaat
    10201 ctccaggggg ctcttccaag aatttcaaca aactgtttgc cgcactgcta ttcatttttt
    10261 cagcttcttt gatcaaaaaa actttttttc ttgattcgta accgctgcga ctgaattccg
    10321 tctgcaaacg tctgatctga tcgactttga tggtttgtcc ttccggttca ataaccaaaa
    10381 catctggatg ttctttcttt tcgatccgct gacaattgtt gcattttcca caaggcagat
    10441 tgtctttcat ttgggtacag aacaggtgct gtgcaagcca tatacctact tcatgttttc
    10501 ctgttccttt ctccccttca aaaagataag catgagcgat acgcccatgc tcaaaactcc
    10561 gttgaagctg ctgatacacg atcggttgca tttgagccaa gtttatttct tgattcatag
    10621 ctcagctcct cctaaaattg atggaatcct tcaacaggta agacgaagac tgtggcaccg
    10681 cctacttcta cttctactgg ataagggacg cccccatcca tcgaaatatc cagtgtaaca
    10741 ggtgtagata caaattgctt tcttgattca cacgtttttt tgatgatttc caatgcttct
    10801 tctacacggt catcttcaat acccacgata aacgtactgt ttccagcctt aaggaagccg
    10861 cctgtcgatg atagttttgt ggcacggata tttgcatcaa tcaattcatt tgataaacgg
    10921 ttgctgtctt tgtcttggat aatcgctaaa ataattttca taaacgttca ccttcctagt
    10981 tttcaaaaaa ttgcggatac tgttcaataa ttgtatgata acttgtttgc agcacttctt
    11041 caaagctttt tcttgcatca actttgtgga ttctttccgg attctcttct gccagcttta
    11101 agtaagcatg acgtacacgc tggtggaatt ccaaaccttc cgaatctaaa cggtcgattt
    11161 gattttgtct gtttttcttg attcttctta atccggtgtc tgaatctacg tcaagataaa
    11221 gggtaaaatc cggcgtagta ccttctgtag caaactcatt caaccgagca atctcggtaa
    11281 cgccgatgcg tcggcctgca ccttgataag caagagaact gtcgacaaat ctatcgcata
    11341 aaacgatttt cccttttgcc agagcaggca gaaccttttc gactagatgt tgtcttcttg
    11401 ctgctgcata aagcagtgct tccgtacgtt catccatccg atcatttttt ggatcaagga
    11461 tcactgctcg gatctcttca gcaatcggaa tgcctcctgg ttctcttgtt tgaatgattt
    11521 ctgtttttgc tacttctttc aaaagagggt aaagttcatt caagatgctg gtttttcctg
    11581 caccatcagg cccctcgatt gtaataaata gtccgttcac attgttgccc ccatctcttg
    11641 tgctgctttt atattgtatc tgaaaaaaca cttaattaga aatttataac tcacgtcttc
    11701 cttcgacagc tttcaataaa gtaacttcat ctgcatactc gatatcagag cctacagaca
    11761 gaccatgtgc tagacgagtc actttgatac ctgcgggttt gatcagacgt gagagataca
    11821 tagctgttgc ttctccctct gttgttgcat ttgttgcgat aatcacttct tttacatctg
    11881 catcatgcag ccgctgcaac agagaagaaa tattgatgtc ttctggacct gtcccttcca
    11941 tcggtgataa cacaccatgg agaacatgat aaagtccgtg atattctcgc attttttcca
    12001 ttgccattac atctttcggc tcttcgacca ctaggataac gcttcgatca cgtgaaggat
    12061 ttctacatat ttcgcaggga tcttcttctg tgatgtttcc gcagatacta caaaaatgca
    12121 aatcgcgttt tacacttaac aaagatttag caaattcatt tactacttca tctttcatat
    12181 cgatcgtata aaaggcgagt cttgtcgctg ttttctgacc gattcctgga agtttcatgt
    12241 agctgtcgat cagtttcgca attggttctg gatattgcat attcattact ccttcagttt
    12301 aaaactaaaa tcctggcaaa ccttttgcgt atttccctaa ttttttctca gtttccacat
    12361 caatttgtgc gatcgcatca ttgactgcca ttacgacaag atcctgaagc atctcgatat
    12421 catcaggatc aacgacttct tcattgattt ggatatcttt catgcgacga tcgcctgtaa
    12481 aaaccgcttt gaccaattca ttggtggctt tacctgtaaa ctctgtttta tttagcagct
    12541 cttgtgcttc tgccatttct ttttgcattt tttgtacttg tttcatcatg ccttgcatat
    12601 tgcccattcc acgcatcata attatctact cctttttatt aatcatctac cactgtaaca
    12661 gcttcggagc caaacagttc ctctgctttt actacaagtg tttcttctgg tacttcttgt
    12721 tcttgaggaa gcaactcgat ttctccttct gagtcttcag aagcaaaaga gttttctgtt
    12781 ccctcttctt gtgcctgcgt caaataattt cttcgaagtt ctggccagct ttctcttgta
    12841 atataaacag gatcaggcgc ataatctttg atcattcggc ttaagttgtt gtgtaccgaa
    12901 agctgcaatt cttcgtcatt tgctgctctt tgacaaacaa tctcataatc aaacgcaatc
    12961 actagtcctg taggtccagc tgcaactggt tcgcttgctt taagcattgc tcgctgagtg
    13021 acagatagac tcatcagcaa gtcttcccat acttctttta cttggttcag atgctgtctt
    13081 gtcgcttccc gcagaacttt atatactcgt tccgtcggta cacgataggt acttgtctgt
    13141 tttttatgat ttgtttgctg taccggctgt acttttgtat tctcaattcc tgtattactg
    13201 cgtagttcct tgatttcgtt tcttagttcc ttaatctctt gttgtagctg ttttactatc
    13261 tgatcatctg caaatgctac atctgctgaa ggaacagaag cagacggtag ctgtgcaagt
    13321 ttgactgtag cgacttccaa ataaatagcg gcactattgg taaaacgaat ttcattttga
    13381 gtatcgctca aaatctttat cattgaaaac agctgatccg ctgaaacagt ttgtgccaat
    13441 tctgtaaatg tacttctgat cgtactcgat tgttcctcaa ctaggttagg tgcttgctga
    13501 tacatcagca agtcacggca gtattgtaac atatcctcta gaaagcgacg tgattctttg
    13561 ccggctgcga gaatttggtt caatgtttcc aatgcttctg gtacttcttt tttcacgcaa
    13621 gcactcaagt aactgtccat catttcgtca gttaagctac ctgtcacttg catggcatca
    13681 tctaaagtaa ttgttccttc actaaacgag attgcctgat caagaatact caatgcatcc
    13741 cgcatcccgc cttctgcaga acgggcaatc acattcaatg cagctggttc atagctgata
    13801 gtcgattctt tcaagataaa ttcaagatgt tcgacaatat ctgccgtatt gatccgttta
    13861 aaatcaaatc gctgagtccg tgaaatgatc gtagctggta ttttgtgagg ttcggtcgtt
    13921 gctaagatga atatgacact ttcttttggc tcttccaatg ttttcaacaa ggcattgaaa
    13981 gctcctgttg aaagcatatg tacctcatca ataatgtaca ctttgtacgc tgcctttgta
    14041 ggcgcataat ttgcccgatc acggataaat cggatttctt cgactccgtt attgctggca
    14101 gcatcgattt cgatcacatc ttcttgcgta ccagcagtaa ttgatcgaca catttcgcat
    14161 tcgttgcatg gctcaccgtc tttactgttc ggacagttga ttgcttttgc gaaaatcttt
    14221 gctgcactcg tctttccggt tcctctgggt cctgtaaaca agtaggcatg tgaggttttg
    14281 tgggaaacaa tagcattttt tagtgtctgt gtcacagctt tttgtccaac gatgtcttcg
    14341 aaacgctgcg aacgccagac tcgataaagt gcttgatatg ccataaggat cccccttttt
    14401 taaaaaattc actatctctt attatacgtg atattaggta aaaaaactac tatccttctc
    14461 ggctttttca ggtatttaaa caatacgcaa ttttccattt caattccgtc tctagtccga
    14521 gaagtttcac atcttcctat ggtataataa ataaacaata attgtttggc actagctgaa
    14581 tcagaggagg aattttttat gggtcttatc aaagcagcaa caagtatggt tggcggcggt
    14641 ctggcagatc aatggctaga agtcatcgaa ccagacaata tgagtgatac aacggtcatg
    14701 acaaaaggtg tcaaagtccg caaagatgac aaacgtggct ccaatcgtaa aggaacagaa
    14761 gatgttttaa cagacggaac agtcgtccat gtctatccaa acatgatgat gcttttggtc
    14821 gatggtggaa aaatcatcga ttataccgct gaggaaggtt attacaccat taaaaatgac
    14881 gcagctcctt ccatgttcaa tggaacactg aaagaagcaa ttgctgaaac ttttgaccga
    14941 ttcaaatttg gcggcgtcac accgcagaaa caacaagttt tttatatcaa tctccaagaa
    15001 ataaagggaa tcaaattcgg tacaagcgcg ccgttgaact actttgacaa tttctataac
    15061 gcagaattat tcttacgggc acatggcact tattctattc atgtcgttga cccgatttta
    15121 ttttatacaa acgcaatccc taaaaacaaa acacaagtgg aaatcaatga tatcaatgag
    15181 cagtatttag ctgaattcct aacagcgcta caatcagcaa tcaatcagat gtctgcagat
    15241 ggtcaacgga tttcttatgt accatctaag agtttggaat taagcaaata catggatact
    15301 gcattggatg attcttggcg tgaattacgt gggatggaga ttgtttctgt ggctgtagcc
    15361 agcatttctt acaccgatga ttctgtgaaa ctgatcaata tgcgtaacga aggggcgatg
    15421 ctaggtgatc ctagcgtacg tgaaggatac gtgcaaggtt ccattgcacg tggtatggaa
    15481 gcagccggaa aaaatgaagc aggagcaatg accggcttta tgggtgtagg tatgggaatg
    15541 aatgcaaatg gttcttatct ttctcaagca gctcaaaata accaagaaca aatcaaacaa
    15601 caagcagaaa aacaaaatca acaaactgca caaggaaatg cggatacttg gacatgtcca
    15661 gtttgcggga cagaaaattc cggaaaattc tgttccaact gcggagctgc caaacctgta
    15721 gaaaacgttc agcctaagct acaaatgcga tgcagtgaat gcaatgagat cgtggatctt
    15781 tccaacggca tccctaaatt ctgtccaaac tgtggtaaac cgtttaaagg aatccccgtt
    15841 aattaattta tttagaaagg tgtgcagctg atggatgttc ttacacataa atgtcctaat
    15901 tgcggcggtc cgttgacttt tgatcccaaa gaccaaaaat ttcattgcga atactgcctg
    15961 aatatctata cagaagaaga agtcagccaa tatgagcaaa agcaaaaaga agcgcgtatg
    16021 gctggcgaag aaagcaaaga agaaccttca cctaccgaag attttacttt tacagcccaa
    16081 gaacagcttg ccgatatgaa tgaagccgag cgcaaagcgg ttgacgaggc aggcggtttt
    16141 tcagatacag cggacgaatc tgcagataat accgcagcga tagatctttt tctttgtccc
    16201 aactgtggtg cagagatcgt gactgatgcg acaactgctg cgacttattg ttactactgc
    16261 cataatcccg tcgtactatc gggacgttta agcggagaat tcttgccaaa taaagtactt
    16321 ccttttgctg tcgagaaaga agaagcaatc aacagatttc tcgcttggac caagaaaaaa
    16381 tggtttgttc ccaaagcttt tttcaataag gatcaaatcg ataagttgac aggtgtgtat
    16441 ttcccttatt gggctacaga tgctgaagta gatggtgcca tgcaggcgaa tggtacagtt
    16501 atccgcatat ggcggatagg tgacatcgaa tacacagaaa cgaagcaatt tgctgtttca
    16561 agagcaggca agctttcttt taaagaacta gtaaaaaatg ccttatcaaa aaatacgcaa
    16621 caaaagatgg tcgaaggcgt gcaacctttc ccgttagata aagcaattga ttttaaaagc
    16681 cagtatttag ccgggttcca agcagaaaaa agagatatcg aatatgaggc aatcaaatca
    16741 cagattcaat ctgaattaaa agactattca gagaagctgt taaaagatac cgcaaatgga
    16801 tatacaacat tgaccggtgt gcgaacttct gcttcaatca caaacgaagt aaataattac
    16861 gtattactgc cggtatggct tgttacttat cgaagcaatg actcaagtaa aaaagtctac
    16921 tactacgcaa tgaatggaca aacaggaaag gtcagcggtg tcctgccgat cagccagaaa
    16981 aaattgggac tgaccgctct tgggattttc gctgggctag cgatcctctt tctgatcgga
    17041 ggttacttga tatgatgaaa aaattttttt tattctttct tttgctagta agtgttggct
    17101 ggccggtggc tactcatgca gatacagcta cggtaaatga tgaggctggg ttattcacaa
    17161 cagatcaaat ccaatctttg gaacaacagg cagaaaagct gaatgaaaaa atcaaaggac
    17221 gagtcttgat cgtaacgact acatctaatt cagaagaacc aagagacttt gctgataatt
    17281 acttaagaga cgcagtcgga aatgatcaaa atggctcagt attgctatta gatatgggac
    17341 agcgtgaaat ctacatttcg acttctggta atatgatcga ttatttgact gatagcagaa
    17401 tcgattcgat cttagatgat gtttatgatc agatgacaaa tgctgcctat tacaatgctg
    17461 caaaagctta cctgtctaaa tcagcaacat atgtggaaga cggagttcca ggaggacatt
    17521 atcgtgtaga cgaagaaact ggcaaaatta cacgctataa ggttttgacg acagtagaaa
    17581 tcatcattgc tgttgttttg gctcttatcc ttagtcttgt cttttatttt gtgactgttt
    17641 cgcgttatca attgaaatca ggcatctaca aatatccatt ccgagaaaaa tccagtatca
    17701 aattaactga aaaaaccgat cgtctgacaa attcttttgt cacgacaaga agaatcccca
    17761 aaagcccacc tcctggaagc ggcggtggcg gaagtactac ccattctagc ggtggcggga
    17821 cttttggcgg tggcggcaga agtttctaaa aattgctaaa tgaatgttcg caaaaaaagg
    17881 gctgtgacaa aaatcgtgtc acagcccctt ttgttgcaaa taaacggcgt tcaaaagctt
    17941 acatctacgg tgataagtcc agccaagtta aaaacataag agactgtttt tacttggctc
    18001 ttcttattac ttgatgcaga acagcttttt cacaacctct tggtaaacgg tgttctccta
    18061 ccaactcctc ggtcacaagt cacaatattc atcaaacatg aaaagctgtt ttttgaaatt
    18121 gttccttgtg actcagagtt aaacggcagg ctcacaacct cttggtaaac ggtgttcaat
    18181 cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccgttt
    18241 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctttgttca tcaaaagata
    18301 atattgtctt ctaacaagtc catattcaaa ataacgatat gatggtcttt aacggtgata
    18361 ataccatctt ttttcagttg ttttaaaatt ctgtttacac tgctagcagt tgagatgcca
    18421 caaaatttgg cgatttcttc attcgtgatc gaaaaatcga tcagcatccc ttcttctgtc
    18481 cgcacaccga ataaatcata taattcgtaa agttgcgtac aaacagctcc cagctttccg
    18541 tttaatagca tttgctgcat ttttttcatc gaataaagca gacgatagcg gtaatactct
    18601 tttacatagt gttgtagctt tgggtcttgg ttgatatttt gccaaaagcg cacacgattg
    18661 attcgataaa actctgcata ttctgattca actcgaatat tatacggcga atcgatgaat
    18721 tgtgaatatt cgtcttttaa aagagagacg atttcaagat ctttgatata gcgaaggtta
    18781 aattctctcc catcatttga aataacactt gcttttaccg ttccctcttt caagatatac
    18841 gtatgggaat cttccatccc ttcgaatgtc aaatatttat gatatccttt gcgtataatc
    18901 ggaaaatcat ttttatccaa atattccttt aagactctac tatccaatga tgctccccca
    18961 tccaaacttt catctaaaaa gatcatacag tactttaaaa ctttttaaag tccaaaggta
    19021 ttttactaat gatattaaca tttgttaagt acctttttcc atcacttatg cgattttttt
    19081 cctttttttt attcgttata ctgcagaagt aaaatttttt aagaatagag ggaaaaaaca
    19141 tgaattactg gatgaacaca atcattaatc gattagaaac agcatatcaa acaagatttg
    19201 atatgaaagc ttctttagtc tttttaaatg atgcgtatca aaactcgatc gaattgatca
    19261 aagcagtgga tgagaatcca acaaacgaat gcgaagaatt tttaaatctg tttatgtcta
    19321 caagagattt atttattcgc caacttgttg atcgctatcc atccaattat cacgatgtag
    19381 aagttcaaat acaaaaatta aaagcatatt cagcttaagg ggaatgtata acatacatgg
    19441 aaaagaaatt atttaataaa tattttattg gaatcacatt gatcaatttt attgtctacc
    19501 tgatttatta tctgttgatg gtcatcatgg cagtcattgc gcaagacacc ttacacgcca
    19561 gcttgagcca agcaggtttt gcttctggga tctatatcat cggaacattg ctggcacgct
    19621 tattcatggg gaaacagtta gaactatttg gtcggaagtt taccttaaga ggcggcgcga
    19681 tcttctatct cctttcaaca ttagcatatc tttatacgcc aactatcggg gcattgtatt
    19741 tagttcgatt cttgaatgga ttcggctacg gtacagtttc tacagctaca aatgctatcg
    19801 ttactgcata tatccctgca tctaaaaaag gggaaggcat caattattat ggattaagta
    19861 ctagtttagc tgccgctgtt gggccatttt taggaatgat tttgctgaat atgactgatt
    19921 ttcgtttcat tatctggttt tcaattgtac tggtcttctt tgttacgatt gcctgccttg
    19981 tctttcctgt aaaaaatatt ccattatctg aagaacaaaa aatatctctt cgctcatgga
    20041 atatcaatag ttttattgaa aagaaagcac ttttcattac aggaattgcc tttttgatgg
    20101 gtctttctta ttcaagtgtt ttatcatttt tatcttctta tacaaaagta atccacttag
    20161 ttgctgctgg gtcatttttc ttcgtcgtat atgctttggt catcacattt acacgtccat
    20221 tgactggaag gattttcgat gtcaaaggag aacaatacgt gatgtatcct agctatttat
    20281 tcttaacagc aggattattt ttactgagtg taacgacgaa tagtgtgaca ttgcttatct
    20341 ctggagcatt agtcggccta ggttatggta cgtttatgtc aaatggacag gctgtttgtt
    20401 taaaaatcgt ccgcgaacat cgaatcagta ttgctttatc tacctacttt attggtttag
    20461 acctaggctt aggcgttggt ccttatttga tggggatgct aaaatctctt acttcattta
    20521 gaggattgta tgtgattgcc ggagtcattc cattgatatg tacagcactc tatcttgtta
    20581 atggccgaaa acatagtcat gaagcagagg gcgaattgat tgaatcattg aaaaatgaaa
    20641 tttgatcttg atgaatagat aaaaaaaacc gttcagatct gaaaataatc tgagcggttt
    20701 tttgagtaaa aatagtttat gattattctt aattaaacat ctatttattt ttaaagcttt
    20761 catttatcga taagaatagt ttaactgttg atttattcca ttttccgttc atctggtacg
    20821 atgccttgac ttagcaacca cttgtggaag aaccattctg ccacaccgat caatagagca
    20881 ggaatcaata ctttcatcca aatatcgact gtatcattca taaaatatag atagagccac
    20941 aagataactg cagctgaacc agcatctgcg atcgtcgctg cgatgttccc tactttacgt
    21001 aagatcaaca gatcccctag cacatagatc atgactacaa taattacccc aacaatcgtt
    21061 gaatccataa agctaacacc gtataaacct gtcagcacga cccacagaat cgcccagatc
    21121 atgattgcct tcactaccaa cgccttcaca tgtttcattt tctgcactcc tttctcctgg
    21181 ataaaaaagt aagattatct aacaagataa ccttttcatt attataccat cggctgagag
    21241 aagcgagcaa agataaggtc ctatctaaaa atgaggaagc cacttagaac attcacgtaa
    21301 taatttatcc gtacagacat tcgatatggt gttcctttac tttcgcatac tgttcttttg
    21361 tcagttcttt acttgtcacc agataatcga tttcatgaaa agcaatggct tgatgcatga
    21421 atttatgtgc aattttttgc tgtgtaacta aaacggcaag ccggtctgat tgttttctaa
    21481 aagctttttt gaccattgcc tctccttcat gagtttctgt tactccttgg ctgaaatcta
    21541 gcccgcgaca actcattatc gctaaatccg cataatatga caatgcatcg ttataggctt
    21601 ttgaaccgtt gattgttgct cgtttcggaa caattgcccc tcccaataca gatacttttg
    21661 ccgttgtata ttcactcaac atagcagctg tgacgatgcc attcgtaatg atctgcaaac
    21721 catcaaatct tgctaggttt ctgactaagt gtaaagaggt tgtactggaa tccaagaata
    21781 agaccatatc atctcgaatc aaagaagaag ctgtatctgc aatcttacgt ttttctgttt
    21841 cttcttctaa tttggtgatc ttcatcaggt aaggcacgtc aatttttcga ttgtcaataa
    21901 gcatggcgcc accgcgaact ctacggatca atccactttt ttccagaaaa acaaggtcac
    21961 gtttaatcgt ggcttcgcta taatttacta actcgatcaa atcggtgact cttgcaaatt
    22021 ggttgttttt tagataattc acaatctcct gctgtctatt ctccacttca ttcaacataa
    22081 aatcacccca tttactttat atagtaacag gaaaaaagag gttgtgacag aagtgattgg
    22141 cttcgagaaa taagaagaaa tttttcgaaa aaactacttc tgaacatcgt gtgtggttaa
    22201 gagggtatga caagactatt gtcataccct cttgaccaca catttttatt tttagtataa
    22261 actagccttg cctactgtgt tcaaaatttc cattttatgt ttgaccactt cttgtacctt
    22321 tttattagct gctggataaa ttgcattcgg ctcgtacatc gatggatttt cactcaatat
    22381 ttctctcaaa gcattgaaaa agacgctttt aagatctgag ctcaaattca ctttggcaac
    22441 tccgtatttt actgcttcgc tgacttctgc gtccggatta cctgaaccgc catgtaaaac
    22501 aaatggaaga tcgatttgtt ggttcaattc tttcagtaag tcgatattta attttggagt
    22561 tctgtttttc ggatacaagc catgcgctgt tccaattgct acagcaagtg tatcgattcc
    22621 tgtttctttc acaaaaatct tcgcttgatc aggatctgta taaatgattg tttcagcacc
    22681 tccttcagca gaaccattat tcccaatcgt tccaagctca gcctccactg agacatttac
    22741 cgcatgagct aacgcaacca cttttttcgt aatctgtaca ttgtcgtcga atgatttcaa
    22801 agacgcatcg atcatcacgg aagaatagcc gtttcgaatc gcccgtaaga catcttctat
    22861 cgtaccgcca tgatccaagt ggataacgat cggcagttta cttgtataag cataggctct
    22921 tactgccgca acaaactcat cacttaaata ggcgatttca tcaggatgaa tttctaagat
    22981 tactggtgct tctaatctct ctgcttcttc gatgattgct ttcatcatat catagctgca
    23041 aatattgaag gctggtactg caaattgatt ctctttagcc actttcaata actctttcat
    23101 tgtatataac attacgcttc cacctcattt gttagatttt catttttcgt ccacttgtct
    23161 tgttttttct ctttcactgt ttttttcaat agtccaagca taaaggctgt agtgactgta
    23221 cctgatgcaa ttgccaagac atacataggt aaattgttca taacatttgg aattagcatc
    23281 acccaaatcc ctccatgagg tgccattgaa gtaactccta ctgccatgga aatccctgca
    23341 gcgactgctg aacctgtcat caaactaggt atcacgcgga tcgggtcaga taccgcaaac
    23401 ggtatagcac cttctgtaat atacgaagcg cctaaaatcc agcatgattg acctgaagtc
    23461 cgttcttctt tggtaaattt atgtttgaat aataaagtgg ctaaagcaat acctagggga
    23521 ggtgtcattc ctgcagccat acaagcagca atcggtgcat ttactccagc tgacatcaaa
    23581 ccgatcgaga aagtcgaagt cgatttattg atcggcccac ccatatcagc tgccatcatg
    23641 atcccgataa gtaatccgaa caatagcccg gatgtatttc ccaagctatt cagccaattt
    23701 gtcaaagcat ccaacaccca tgtgatcggt tgttcaatca caaataccat caaaagccca
    23761 ataattaaga tcgataaaaa ggggacgaca atcagctgat aaatagcaga aaattctttt
    23821 tttacgctaa ttttatttgc cagaaaatca cagatatacc ctgctagcac gccaccaatc
    23881 attccgccaa gaaaacctga accacctgta gaggccagta atccggcaac aattcctggg
    23941 gcaaaacctg cttttccagc aatcgatgta gcaatccccg cgcctaacgc aggaaccatc
    24001 atggcaaacg ctgtatctcc tccgatcctt gaaaatgcag ctgctaacac attatagtct
    24061 gctgagttgg gatcagccgc tgtaatccca aaggcaaaac tcagtgcgat gatgattccg
    24121 cctgcgatca caaatggaag catgtaattc acaccattca tgaaatgatt atagattgtc
    24181 ggctttttct catttgcagc tggttctttt tcattagaca caacgaatac gccaatatct
    24241 ctcaatgctt cgtctattac tacttgcggt tctttgattg ctcggcttgt agagacacgc
    24301 tttacttttt tcccggcaaa tcggtccaat tcgatttctt tatctactgc aagaatgatt
    24361 gcatctgcct ctttgatttc ttgttcagat aactgatttt ctgtttttac accttgtgtt
    24421 tcgaattttg cttgatggtt tagatttctt gctgttactt ctaatttttc tgccgacata
    24481 taagtatgag caattccagt cgggcaagca gttacaccaa cgattttcat gattattttc
    24541 ctcctttttc ttcactatcg atactcggac aaagactcta ctattttcat tcttgactaa
    24601 tctgtctgat gatatctgct ggcgtagtgg cttcttttat tccctttcta aaatctgcat
    24661 tcatcaatcg tctggagagt gtgctgagaa tctctagatg catatctgct gatttttcag
    24721 gaactacgat ggcaaaaata tattcaattg gcatttcatc tatcccgcta tagtccgtta
    24781 atttggctgt atgaaaaaaa cacagtgctg gttttgtcac atatttgctt tttgcatggg
    24841 gaatcccaaa tccatcttct attcctgtgg agatctcctc ctctcgtttg aatagtgcct
    24901 taacgacttt ttgaacattt cttaccttat ttgttttttt caacacatcc cctattcgct
    24961 caaataatga tccttttgaa tctatcgatg gatcgaaaac aatcagttct tctgcgatca
    25021 attgatcaaa cctcataaaa aacctctccc ccacaaaaaa tgttaacgtt ttcttggaac
    25081 tgaggtcagt atagcatcgc tagctgtacc gttcaatatt atgaatgaac agaggaaaaa
    25141 gacccatcat ccgcgtgtat aagtcattaa aatgaacgat ctagctcaca aacaatcgca
    25201 agtctataac aaaacaaaaa gccttccttg aaaggaaagc ttagcttcta attttaggta
    25261 aatggttttt tacttcgaaa taacccctct tcatttcgtt aatataaggt gtttttatca
    25321 agtggataat taaaactata tactagtttt tcattgtttt gtgcattatg atcctacgac
    25381 tttttcagca tcaaacgaag ttttaaattg gcaaaaaaca taaaaaaaca gactatccct
    25441 atcagatagt ctgctaaacg ttgagttatc agcgttttta ttattccgca gcgtttgcgt
    25501 ctttgagacc gtattttttg ttgaaacggt ccacacgtcc gtctgcttgt gtgaattttt
    25561 gacgtccagt gtagaatgga tgtgagtcag aagttacttc gacacggatg actgggtatg
    25621 tattaccgtc ttcccattca actgtttctt gtgaagtttt tgttgaacct gacaagaatt
    25681 tgaaaccagt tgttgaatcc atgaatacta ctgggtggta atctggatgg atgttttctt
    25741 tcatgatttt ttcgctcctt tgccctgctc catttgctga accagagttg atttgtcgat
    25801 ttacaacatt aacatcttaa catgtttcag tcaaggttgc aattatcttt tcagatttct
    25861 ggtcggattt tttttaccaa aggagacatc ttgaaattct ttgaaaaatt gctgattgtt
    25921 ttttgtttta cgcaaaaagc ggatcagttg ttccgtatat tctaatgaat ctccagtcat
    25981 attttttcga agtttccaaa tttcttctaa ttcttcactt ggcatcaata attcttcttt
    26041 tcgggtacct gattttttga tatcgatggc tgggaagatc cgacgttcag ataattcacg
    26101 tgaaagttgt aattctagat ttccagttcc tttgaattct tcatagatca cgtcatccat
    26161 acggcttcct gtatcgacta aagcggtcgc taaaatcgtc aaactgccac cttcttcgat
    26221 gtttcttgct gccccgaaaa atttcttagg tttatacaat gcggctgggt caatcccgcc
    26281 actcaatgta cgtccgcttg gtggaacgac taagttatag gcccgagcaa gtctagtaat
    26341 actatccatc aagataacga catctcgttt atcttcgact aaacgcattg cgcgttccaa
    26401 tactaattca gaaactcgag tatggttttg cggctgcaga tcaaatgtag aagataccac
    26461 gtcgcctttc acgcttcgtt ctagatccgt tacttcttcc ggtcgttcat caatcagtag
    26521 aacgattaat tctacttccg gatagttttc ggtgatacca tttgcgattt cttttaatac
    26581 agaagttttc cctgcttttg gcggtgcgac gatcaatcca cgctgaccaa aaccgattgg
    26641 agaaaagata tcgatcattc gggtagatag tctttgcggt gttgtttcca gttgaagctg
    26701 tctttgcgga taaagaggtg ttaaggctgg aaaatgcgga cgttcttttg cttcttccgg
    26761 atcttttcca ttaacgcttt ccacgtgcat caaaccataa taacgttccg attctttagg
    26821 tggacgggct tttcctgcca ctttgtcccc atttctcaaa ctgaatcgtc tgatttgtga
    26881 ggcagaaata tagatatctt ctgcacttgg tccataattg attggccgta aaaaaccgta
    26941 accatcttga ccaacgatat ccaatattcc ttccatataa taaaatcctt gtttctctgc
    27001 ttgtgcacga atcacagcta gagataattc ctttttattc atcttactgt aataaggaat
    27061 tttaaattct ttggcataac tataaacttc ttttaacgtt ttgttctcta gttctgccat
    27121 tgtcaaataa tcgctcattc agctcccact tcctctatca tttcaattga tgcccctaag
    27181 gctgttagtt tctcgataat atgatcataa ccgcgtaaga tataatctac attgtaaatc
    27241 gtcgttgtcc cttctgccaa tagtccagca atcactaaac aagcacctgc ccgaagatca
    27301 gaagccacga cttctgtgcc gtgaagttta tttggaccat tcacgatgat catatttcct
    27361 tccacggtaa tatcagcgcc cattcgtgct agttccggca catggttcac acgtttcgca
    27421 tagatcgtat cgatgatttc tgaagtgcct gtagtcatca atagtaacgc agtaagtggc
    27481 tgctgcagat ctgtggcaaa tcctggataa ggataggtca tgatgtttgc gggttttaat
    27541 tctttcgagg ggtaaacttc gatttcatct tcgctgattt tcatatggac accaatttcc
    27601 tgcaattttg ctataaagct ttccaaatgt tcaaagatca catttttcac tttgatacca
    27661 tttcctactg cagctgctaa tgctagatag gttcctgctt cgatgcggtc aggaatcatg
    27721 aaatgccgac agccatgtaa tgtttcgact ccttcgatac ggatcacatc tgtacccgct
    27781 cctcgaactt ttgcccccat attattcagt aaagttgcca catctatgat ttccggttct
    27841 cgtgcagcgt tttcaattac tgtctgtcct tttgctttta cagcagctaa catcacattg
    27901 atcgttgctc cgactgaaac cacgtccatg aaaatccgat tgccacgcaa tcctttattt
    27961 tctgtccgta aatacatggc accatgttca tttgtcactt cagcacctag agcttcaaat
    28021 cctttgacgt gtaggtcaat cggtctaggg cctaaataac agccacctgg caggcctact
    28081 accgcttcgc caaatttacc aagcagtgtt cccataaaat aataagaagc acgcaaacta
    28141 ttgatttttc cgtgcggcat cggaatcgat acgacttctc gaggatcgat ttctagtatg
    28201 ttgttggaaa aatggacagt tgcgcccatg atctctaaaa tttcaattaa tgaatgaaca
    28261 tcttgtatgt ccggcacacc ttccaagatc actggggaat cagctaagat tgcagctgga
    28321 atcaatgcga ctgcactatt ttttgcaccg ctgatcgtta cttcaccagc taaaggtcgg
    28381 tttcctttga ttacgatttt tttcatctgt ttccaccttg tttattaatc ttttttcatg
    28441 gatatgcttg tattttatca tagataaaaa aaagaaacga gcatggtctg ccgttttaag
    28501 taaaaaagaa tggagagcga cttcaaaagt cgttctccat tcctatatat cgtatctgtt
    28561 atgtggatgc ttacgctttg ttagctgaac cgaaccagtc gatatgagat ttagcatctt
    28621 caacgattgc tgctttacct ggagctaata atttacgagg gtcgaatcct ttaccttctt
    28681 tatctttacc agcttcgata tatttacgag tagctgcagc aaatgataat tggaattcag
    28741 tgttaacatt tacttttgag atacccattg agatagcttt ttcgatttga tcttgaggaa
    28801 tacctgatcc gccatgtaat actaaaggca tgtctggacc tacagcatca gcaattgctt
    28861 gtaagtggtc aaaagctaat cctttccagt tttctggata ttgaccatgg atgttaccga
    28921 taccgcaagc taagtagtcg atacctgtag caaccatttg tttgcattct tcaatatcag
    28981 ctaattcgcc gttaccgatg attccgtctt cttctccgcc gattgaacca acttcacatt
    29041 caactgagat accttttgca tgtgcttttt caacaacgtc tttagctaat ttcaagtttt
    29101 cttcaaatgg tagatgtgaa ccatcgaaca ttactgaagt atagcctaat tcgatacatt
    29161 ccaaagcagc ttcgtaatca ccatggtcta agtggatagc aactggaaca gtgatgttca
    29221 ttgcttcaac taaatcagtg attaaatctt tacagatttt gtaaccaccc atgtatttag
    29281 ctgcacccat tgatgtttgg ataagtactg gtgcttgttt tgcttcagcc gcttcaagga
    29341 ttgcttgagt ccattccaag ttgtttgtgt tgaatccgcc gacagcgtaa ccgcctttac
    29401 gagcagcttt taaaaattca gctcctgata ctactggcat aataaaattc ctcctaagtt
    29461 ttgtaaaact aaatttgtta agacaattct taaccaacga ccctatttta ccaaacattt
    29521 cggtagtttt ctactaaaat caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa
    29581 taaaatatcc aatagcgctt taatcgaaaa aagtcttttg ctttctatct tttttcctac
    29641 aaataataac aaatcagaaa aagagaaaag tgacaagaaa attgcttatc cccttctctt
    29701 gctacttttc tctttctatc aagttcaaaa attattttgt ttcgtgatct gctaatgatg
    29761 ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg acgagaaatc aattctgggt
    29821 ggaattggca tcccacaaag aattttttct ctggcagctc tacgatttct actaaacggt
    29881 tatctggtga gacacctgaa aagactagcc cttgttcttc aaacaattga cgatatttgt
    29941 tattgaactc atagcggtga cggtgacgtt cttgcacaac atcggctcca tcatatgctt
    30001 cagccgtctt cgttcctttt ttgattttac atgggtataa tccaaggcgt aaagttccac
    30061 ccaaattctc gatgttttcc tgatcagcca ttaaatcaat aatattgttt gctgtatctg
    30121 gattcgtttc agctgatgac gcatcttcta atccaacgac gttacgtgca aattcgacac
    30181 aagccatctg cataccaaga caaataccta aaaacggaac gtcattttcg cgagcataac
    30241 gaatcgcttc gattttccct tctactccgc gatcaccaaa tccaccagga accaagattc
    30301 catctgcgtc tttcaaacgt tcttctacat tttctctcgt taattcttgt gctttgatcc
    30361 aatcgatctc gatgtctgaa ttataggcga aaccagaatg tttcaatgct tcaactactg
    30421 agatatatgc atctggtaac tcgacatatt taccgacaag cgcaatccgt acttttttct
    30481 tcaagttcaa gacacgttct tctaattctt tccattctgt catgtctgct tctggtgtat
    30541 ctagttttaa atggtcgcaa acaatttgat ccattccttg tgcttgtaaa actaatggga
    30601 tggagtaaag tgtatctaca tctggcgact cgataactgc ttctggttcg acatcacaaa
    30661 attgtgccaa tttgtttttc acgctttgag aaacaggttg ctctgtacgt accaccaaaa
    30721 tattcggttg gatacctaaa ccacgtagct cttttacact gtgctgcgtt ggtttagttt
    30781 tcatttctcc agcagctttc aagtaaggaa tcaatgttgt atggatatac ataacattat
    30841 ctgtaccgac atctgctttc atttgacgaa gtgcttcaag gaatggcaaa gattctatat
    30901 cccctactgt tccccctact tctgtaatga tcacatctga atcagtcatt ttagctgctc
    30961 gcatgatttt ttctttgatc tcatttgtaa tatgaggaat aacttgaaca gttgctccca
    31021 agtattcgcc cttacgttct ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt
    31081 tggagtattt gttcaaattg atgtcaataa aacgttcgta gtggcccaga tccaaatctg
    31141 tttcagcacc atcatctgtt acgaatactt ctccgtgctg atatgggctc atcgtccccg
    31201 gatccacgtt gatgtatgga tcaaattttt ggatagttac ttttaagccg cggtttttca
    31261 ataagcgtcc taaagaagct gcaacaatcc cttttccgat tgatgaaacg acaccgccgg
    31321 tcacaaaaat atattttgtc ataagttcaa aaactccttt ttaatttatg tgcaggaaaa
    31381 gtctcagaca ataaacaaag ctccctattc ttcaataaga atagggagcc gaaatttctg
    31441 taaacttctg accctctaaa agggtgccca aggtgtataa tacaagctga ctaagggaaa
    31501 gtcaagtact caaatgaata ctatccgctt acaatttttt ctttttcgat atcctttgag
    31561 aaattatttc ttaagtcctt tatgagtgca aaaaaaaatt taacccacat aataagcttg
    31621 atataaacat agctaacgtt aaacctaagc aaatcagaaa atcgttactg aaaaatttta
    31681 agtgaaataa tagataaaaa aataaaacaa acattatgat agataatatc ccacctaaca
    31741 aagaactact ttttcctact tgcagcaaca gtttatatag cttatcgtag aaacaatctt
    31801 tttctttcac ccttatttac ctcctttacg taaaaaacgt agtccacagt tctcggattt
    31861 ttggcatatc ttttctatta aataatatca aaaacaaaac aattccacta attttttcaa
    31921 agatgacctt tttttcaaca aaagtgcctt gtagggattc attgaaggta acgaatcaca
    31981 taagtaaaaa ataaggagat gcgacaaaag tcgtgcctcc ttatttctaa ataaacggtg
    32041 ttctcctgcc aactcctcgg tcacaagtca caatatccat caaacatgag aagctgtttt
    32101 tgaaattatt tcttgtgatt cggagttaaa tggtaggctc acaacctctg gataaacggt
    32161 attcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt
    32221 tcacaaaatt tttcttaata ctcgaaacta aacgtctttt tcaaaacctc ttggtaaacg
    32281 gtgttcaaaa gcttgcatct gcggtaataa gttcagccaa ttcaaaaaca agaaaagctg
    32341 tttttgcttg gctattctta ttacttgttg cagggcagct ttttcacaac ctctccacta
    32401 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
    32461 tatttttccg ttagtttctc taatactcag gtcaaaacgc tgatttcaca gcctctaatt
    32521 cacggtattc ctgttttaat aaagttctag atattgttct ctttcccact cggaaacagt
    32581 ttgtctgaat gccgcccatt cgagacgttt tgcttcaaca aaatttgaga agatatgttg
    32641 tcctaatgcg tcaatcatta cattatcctt acgcaattct ttgatagcat tatggatagt
    32701 tgaaggtaag tcctggatat gtgcagcttc tctttcttcc tcatccatta cataaatgtt
    32761 acggtcaaca gctgctggcg gtgtgatctg attacggata ccgtctagtc ctgcttgcaa
    32821 caatactgcc attgccaagt atgggtttgc agatggatca actgaccgca attccaaacg
    32881 tgtggataaa ccgcgagatt ctggtacacg tactaatggc gaacgattac ggccactcca
    32941 tgcgacataa acaggcgctt cataacctgg aacgagacgt ttataagaat tgactgttgg
    33001 attgcaaacg gctgtgtagg cacgagcatg tttcagcaaa ccacctaaga aatgataagc
    33061 tgtttcactt agttccaatt caccgtcttt atcatagaaa acatttcctt catcattgaa
    33121 caaagacata ttgcaatgca taccagaacc attgatccca aataacggtt tcggcatgaa
    33181 agtcgcgtga agtccatgct tgcgtgcaat cgttttgaca acgagcttga aggtttggat
    33241 attatcacaa gcatctacca catctgcata tttaaagtca atttcatgct gtcctggcgc
    33301 tacttcatga tgtgaagctt ccacttcaaa gcctagactt tctaattcca acacgatgtc
    33361 acggcggcaa ttttcaccta aatctgttgg tgcaaaatca aaataaccac ctttatcatt
    33421 caaggtcgtt gtgatcccac cgtcttcgtc taatttaaat aagaagaatt ctggttcagg
    33481 tcctaaattg aaagaggtaa aaccaaggtc gcgcatatct ttcaatgctc gtttcaaatt
    33541 cccgcgagga tcgccggcaa atggtgtccc atccggatta tagatatcac aaattaaacg
    33601 agcaactttt ccatgagcac tttcccacgg gaagatcatc caagtggaga gatctggata
    33661 caaatacata tcgctttctt caattcgaac aaatccttca atcgaagaac cgtcaaacat
    33721 cattttattt tctaatactt tgtcgatctg gctcactgga acttctacat ttttgatcgt
    33781 acctaagatg tctgtgaaca tcaatcgtaa aaatcgtacg ttttcttcct caatgattcg
    33841 tttgatttct tcacctgtta tctgtttctt taccatggta tccctcttcc ttttttaatt
    33901 tattcagtca aagaattgtc tttgcaatcc ttgcgactga taatgacctg ttttgcttaa
    33961 cccaccttgc gccaaaatct catcacgaaa aatttgtcga acgtcatgat ctgtcaacgg
    34021 cttcttgttt tttgcttctt gttttttcgc ctgtgccatt tcgtatacac gtttgatccc
    34081 agccatattc aatccctctg aaagatagtc tttaatctca agcaacatat caatatcatt
    34141 cagcgaatac atacgtcggt ttccttcgct tcgttccgga tgaactaaat cttgttcttc
    34201 ataatagcga atttgccgag cagacaaatc agtcagcttc ataactgtac caattggaaa
    34261 tacggacata gatcggcgca attccttttc tcccatcaga cgtccccttt cgttttataa
    34321 gttggctaaa gtgtaccaat taaaaaaaat attgtcaact gcatatgtta ggttttctaa
    34381 catgagatct ctatttttta taaaataaca aaaaaataag aacacaaaaa aggtatctag
    34441 ttgattttcg taccagatac cttttatttt ttattatact gtactatcct ttattagtgt
    34501 ttttatt
//
LOCUS       DADXTG010000016        34024 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.016, whole genome shotgun sequence.
ACCESSION   DADXTG010000016 DADXTG010000000
VERSION     DADXTG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.016"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(13..594)
                     /locus_tag="IWI57_000642"
     CDS             complement(13..594)
                     /locus_tag="IWI57_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2448808.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(727..1410)
                     /gene="rpiA"
                     /locus_tag="IWI57_000643"
     CDS             complement(727..1410)
                     /gene="rpiA"
                     /locus_tag="IWI57_000643"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBC2448809.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1729..2142
                     /gene="rpsL"
                     /locus_tag="IWI57_000644"
     CDS             1729..2142
                     /gene="rpsL"
                     /locus_tag="IWI57_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBC2448810.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2226..2696
                     /gene="rpsG"
                     /locus_tag="IWI57_000645"
     CDS             2226..2696
                     /gene="rpsG"
                     /locus_tag="IWI57_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBC2448811.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2790..4877
                     /gene="fusA"
                     /locus_tag="IWI57_000646"
     CDS             2790..4877
                     /gene="fusA"
                     /locus_tag="IWI57_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBC2448812.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5049..6236
                     /gene="tuf"
                     /locus_tag="IWI57_000647"
     CDS             5049..6236
                     /gene="tuf"
                     /locus_tag="IWI57_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBC2448813.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6643..6951
                     /gene="rpsJ"
                     /locus_tag="IWI57_000648"
     CDS             6643..6951
                     /gene="rpsJ"
                     /locus_tag="IWI57_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBC2448814.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            6982..7611
                     /gene="rplC"
                     /locus_tag="IWI57_000649"
     CDS             6982..7611
                     /gene="rplC"
                     /locus_tag="IWI57_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBC2448815.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7639..8262
                     /gene="rplD"
                     /locus_tag="IWI57_000650"
     CDS             7639..8262
                     /gene="rplD"
                     /locus_tag="IWI57_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBC2448816.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8262..8552
                     /gene="rplW"
                     /locus_tag="IWI57_000651"
     CDS             8262..8552
                     /gene="rplW"
                     /locus_tag="IWI57_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBC2448817.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8591..9424
                     /gene="rplB"
                     /locus_tag="IWI57_000652"
     CDS             8591..9424
                     /gene="rplB"
                     /locus_tag="IWI57_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBC2448818.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9467..9745
                     /gene="rpsS"
                     /locus_tag="IWI57_000653"
     CDS             9467..9745
                     /gene="rpsS"
                     /locus_tag="IWI57_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBC2448819.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9768..10115
                     /gene="rplV"
                     /locus_tag="IWI57_000654"
     CDS             9768..10115
                     /gene="rplV"
                     /locus_tag="IWI57_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBC2448820.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10129..10785
                     /gene="rpsC"
                     /locus_tag="IWI57_000655"
     CDS             10129..10785
                     /gene="rpsC"
                     /locus_tag="IWI57_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBC2448821.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10788..11222
                     /gene="rplP"
                     /locus_tag="IWI57_000656"
     CDS             10788..11222
                     /gene="rplP"
                     /locus_tag="IWI57_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBC2448822.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11212..11400
                     /gene="rpmC"
                     /locus_tag="IWI57_000657"
     CDS             11212..11400
                     /gene="rpmC"
                     /locus_tag="IWI57_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBC2448823.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11425..11691
                     /gene="rpsQ"
                     /locus_tag="IWI57_000658"
     CDS             11425..11691
                     /gene="rpsQ"
                     /locus_tag="IWI57_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBC2448824.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11749..12117
                     /gene="rplN"
                     /locus_tag="IWI57_000659"
     CDS             11749..12117
                     /gene="rplN"
                     /locus_tag="IWI57_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBC2448825.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12154..12462
                     /gene="rplX"
                     /locus_tag="IWI57_000660"
     CDS             12154..12462
                     /gene="rplX"
                     /locus_tag="IWI57_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBC2448826.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12490..13029
                     /gene="rplE"
                     /locus_tag="IWI57_000661"
     CDS             12490..13029
                     /gene="rplE"
                     /locus_tag="IWI57_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBC2448827.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13048..13233
                     /locus_tag="IWI57_000662"
     CDS             13048..13233
                     /locus_tag="IWI57_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBC2448828.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13269..13667
                     /gene="rpsH"
                     /locus_tag="IWI57_000663"
     CDS             13269..13667
                     /gene="rpsH"
                     /locus_tag="IWI57_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBC2448829.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13699..14235
                     /gene="rplF"
                     /locus_tag="IWI57_000664"
     CDS             13699..14235
                     /gene="rplF"
                     /locus_tag="IWI57_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBC2448830.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14396..14752
                     /gene="rplR"
                     /locus_tag="IWI57_000665"
     CDS             14396..14752
                     /gene="rplR"
                     /locus_tag="IWI57_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBC2448831.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14773..15273
                     /gene="rpsE"
                     /locus_tag="IWI57_000666"
     CDS             14773..15273
                     /gene="rpsE"
                     /locus_tag="IWI57_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBC2448832.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15288..15467
                     /gene="rpmD"
                     /locus_tag="IWI57_000667"
     CDS             15288..15467
                     /gene="rpmD"
                     /locus_tag="IWI57_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBC2448833.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15511..15951
                     /gene="rplO"
                     /locus_tag="IWI57_000668"
     CDS             15511..15951
                     /gene="rplO"
                     /locus_tag="IWI57_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBC2448834.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            15951..17246
                     /gene="secY"
                     /locus_tag="IWI57_000669"
     CDS             15951..17246
                     /gene="secY"
                     /locus_tag="IWI57_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBC2448835.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17308..17955
                     /locus_tag="IWI57_000670"
     CDS             17308..17955
                     /locus_tag="IWI57_000670"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBC2448836.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18146..18364
                     /gene="infA"
                     /locus_tag="IWI57_000671"
     CDS             18146..18364
                     /gene="infA"
                     /locus_tag="IWI57_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBC2448837.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18397..18513
                     /gene="rpmJ"
                     /locus_tag="IWI57_000672"
     CDS             18397..18513
                     /gene="rpmJ"
                     /locus_tag="IWI57_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBC2448838.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18531..18896
                     /gene="rpsM"
                     /locus_tag="IWI57_000673"
     CDS             18531..18896
                     /gene="rpsM"
                     /locus_tag="IWI57_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBC2448839.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18924..19313
                     /gene="rpsK"
                     /locus_tag="IWI57_000674"
     CDS             18924..19313
                     /gene="rpsK"
                     /locus_tag="IWI57_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBC2448840.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19393..20331
                     /locus_tag="IWI57_000675"
     CDS             19393..20331
                     /locus_tag="IWI57_000675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBC2448841.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20359..20739
                     /gene="rplQ"
                     /locus_tag="IWI57_000676"
     CDS             20359..20739
                     /gene="rplQ"
                     /locus_tag="IWI57_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBC2448842.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21011..21850
                     /locus_tag="IWI57_000677"
     CDS             21011..21850
                     /locus_tag="IWI57_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBC2448843.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21826..22695
                     /locus_tag="IWI57_000678"
     CDS             21826..22695
                     /locus_tag="IWI57_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBC2448844.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22685..23482
                     /locus_tag="IWI57_000679"
     CDS             22685..23482
                     /locus_tag="IWI57_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBC2448845.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23685..24431
                     /gene="truA"
                     /locus_tag="IWI57_000680"
     CDS             23685..24431
                     /gene="truA"
                     /locus_tag="IWI57_000680"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBC2448846.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24886..27555)
                     /locus_tag="IWI57_000681"
     CDS             complement(24886..27555)
                     /locus_tag="IWI57_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBC2448847.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(27793..27981)
                     /locus_tag="IWI57_000682"
     CDS             complement(27793..27981)
                     /locus_tag="IWI57_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBC2448848.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28251..28613
                     /locus_tag="IWI57_000683"
     CDS             28251..28613
                     /locus_tag="IWI57_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBC2448849.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            28665..30347
                     /locus_tag="IWI57_000684"
     CDS             28665..30347
                     /locus_tag="IWI57_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBC2448850.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            30464..32500
                     /gene="recG"
                     /locus_tag="IWI57_000685"
     CDS             30464..32500
                     /gene="recG"
                     /locus_tag="IWI57_000685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBC2448851.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            32549..33550
                     /gene="plsX"
                     /locus_tag="IWI57_000686"
     CDS             32549..33550
                     /gene="plsX"
                     /locus_tag="IWI57_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBC2448852.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            33672..33914
                     /gene="acpP"
                     /locus_tag="IWI57_000687"
     CDS             33672..33914
                     /gene="acpP"
                     /locus_tag="IWI57_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBC2448853.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        11145 a         6248 c         7339 g         9292 t
ORIGIN      
        1 cgtttttagg tattagatgc ataattgctc gatttctttt tcaggtaaat tgatatattt
       61 ataacatgtt ccaactgaaa caccgcattt tgaagaaata aattggatcg tttctttttt
      121 ttcataatag agacgtcgga tttttttgat cgtttttgca tcgatttttg gtcttccgcc
      181 gatttttcct tttttacgag cttcatctaa tccaactaat gtacgttctt tgatcaatgc
      241 gcactccatg tttgctaggt tttccattaa ttggaaatag atttttccca tttcgcgcgt
      301 atcgattcct tcatcaagac taatcaagtg cagcccgcga tctttgaata attgcgtgag
      361 ttctgttaat tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc
      421 gccttgttgc atgtcagcga tcactgactc caaatgaaca gcagtagaat cattttgttc
      481 cacttcatat gtctcatgga aaatttcttg acaaccaaag tcagataatt gatcaacctg
      541 agttgttaaa tcaacgtcat ttatggttgt gcgtgcataa ccaataattt tcatggtgat
      601 tcctttctag cagtgatttt ctaactggat ataacagtaa aaatgtagca taaagtacgt
      661 caaaaagata gttttttcgc aaattttccg ctgtcttttt ttattggatg atcaagcagt
      721 ttttctttat ttacgacgga ttgcgtcaag aatctcaacg cctttttctg agccgacaac
      781 tactttagaa accatgttta ggaaaagccc atgttctact accccaacaa gttcatctaa
      841 ataggtagct aagctttctg gctgttcgat gacttctaga tgcagatcaa tgatgtaatg
      901 accaccatct gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg gttggaatct
      961 tttttcttca aaaatatgaa gtagctgttg ggagccataa gggatcactt caactgggag
     1021 tgggaacttc ccaagtttat cgactaattt agaagaatca acgatccaaa tcgtttcttt
     1081 tgagtacgta gcgactattt tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg
     1141 aaaatcagca ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc
     1201 aatactcttt aacggtattc ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt
     1261 aactcctgtg atacgtaaac cttcttcacg catacggcga ccaatctctt ctaccatata
     1321 ataggcggtt gaaccggtcc cgagacctac gatcatcccg tcctttacga atttcgcggc
     1381 ttcaatgcca gctaattgtt ttaaattcat ctttgcgtcc accttttttt agtttgagca
     1441 tcgtgctttc ctcactattt tagcgtgatt tacggtaaaa agataccatt cttttttatt
     1501 ccaataaagc tttttcaaaa aaaattgcat ctcttgttga catgtttttt cattcgtgat
     1561 attctaacaa aggtgcttta tctacaggtt tctgtgaaga aacgtgctga ctgtttatca
     1621 aagcatattt gataaggatg cagacattgc atttattttt tatcacaaaa aaagaaccac
     1681 ctggatgtgt ggaactagac gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt
     1741 aaccaattag tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc acctgctttg
     1801 aacaaaggat ataacagctt taagaaaact caaactaacg taaactcacc tcaaaaacgt
     1861 ggggtagcaa ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa
     1921 tatgcacgtg ttcgtttgtc aaacttaatt gaagttactg cttatatccc agggattggt
     1981 cataacttac aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca
     2041 ggggtacgtt accatatcgt tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa
     2101 caaagccgtt ctaaatacgg aactaaaaaa cctaaagctt aattttaatt attaaacaaa
     2161 tgaaacttgc ttctactata tagaagtaat tactatgaac aatatttcgg aaggaggagt
     2221 tacggatgcc acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat cctatttata
     2281 actcaaaatt agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg
     2341 cagctaacat tatctataat gcctttgact tgatcaaaga atctacaggt aacgatccat
     2401 tagaagtgtt tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc
     2461 gtgttggggg ttcaaactac caagtaccag ttgaagttcg ccctgaacgc cgcgttactc
     2521 taggtcttcg ttgggtagta aactacgctc gcttgcgcgg tgaacacaca atggaagaac
     2581 gcttagcaaa agaaatcatg gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg
     2641 aagacactca taaaatggct gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt
     2701 ctctctgttt ggtcggcttt tataagtcga cctcagatag tgtccttatt tttagcaact
     2761 agatagtaaa gagaggagaa acaaggagaa tgaccagaga attttcttta caaaacactc
     2821 gtaatatcgg tatcatggcc cacatcgatg ctggtaaaac gactgctact gaacgtatct
     2881 tgtactatac tggtaaaatc cataaaattg gtgaaactca tgaaggagct tcacaaatgg
     2941 actggatgga acaagaacaa gaacgtggta ttacgatcac ttctgctgct actacagcac
     3001 aatggaaggg tcaccgaatc aacatcatcg acacaccagg acacgtagac ttcacagttg
     3061 aagttgaacg ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat gcacaatcag
     3121 gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac aacttatggt gtacctcgta
     3181 tcgtatttgc aaacaaaatg gacaagatcg gtgctgactt cttatattca gtatcaactt
     3241 tacatgaccg tttgcaagca aacgctcacc caatccaatt accaattggt gcggaagacg
     3301 acttcacagg aattatcgac cttgtaacaa tgaaggctga aatgtataca aacgatcttg
     3361 gaacagagat cgaagaaaca gaaattcctg aagaataccg cgaattagct gaagaatggc
     3421 gcgaaaaatt ggttgaagct gtagctgaaa ctgatgaaga acttactttg aaatacttag
     3481 aaggtgaaga aatcactgaa gctgagttaa aagaaggtat ccgccgcgca acagttaacg
     3541 tagaattcta cccagtacta tgtggttcag catttaagaa caaaggggtt caattactat
     3601 tggacgctgt tcttgattac ttgccatcac cgttagatat cccagctatc aaagggattg
     3661 accctaaaac tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct ttctcagctt
     3721 tagcatttaa agttatgact gacccattcg taggtcgttt aactttcttc cgtgtgtact
     3781 ctggtgtttt acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg
     3841 ttggtcgtat cctacagatg catgccaact ctcgttcaga aatctctgaa gtttacgctg
     3901 gagatatcgc tgctgctgtt ggtttgaaag acacaactac tggggacaca ctatgtgatg
     3961 aaaagagtct agttattctt gaatccatgg aattcccaga accagttatc caagtcgcta
     4021 ttgaacctaa atcaaaagct gaccaagata aaatgggtgt ggctttacaa aaactatctg
     4081 aagaagatcc tactttccgt gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg
     4141 gtatgggtga acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg
     4201 aagctaacgt aggtgcgcct caagtatctt accgtgaaac tttccgtgct ggcactcaag
     4261 ccgaaggtaa gtttgtacgt cagtctggtg gtaaaggtca atacggtcac gtatggattg
     4321 aatttacacc aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg
     4381 ttgttcctcg tgaatacatt ccagctgttg aaactggttt gaaagatgct atggaaaatg
     4441 gtgtcttagc tggctatcca ttagtagata tcaaagctaa actttacgat ggttcatacc
     4501 atgatgtcga ttcaaatgaa actgccttcc gtgttgctgc ttctatggca ttacgtgcag
     4561 cagctaagaa agctaatcca gtaatccttg aaccaatcat ggctgtagaa gttgttatcc
     4621 ctgaagatta tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag
     4681 gtatggaagc tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa
     4741 tgtttggtta tgctacaact cttcgttctg ctactcaagg tcgtggtaca tttacaatga
     4801 catttgacca ctacgaagat gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg
     4861 gcggaaaagc tgaataattt cttgaaaccg gatagtcctg caaagagatt atccaaagag
     4921 actatatttt taatatagtt tcgtgtaaaa taattatcga tgatatgggc atgtggtgta
     4981 ccttgtgtac atcatatacc tatcagacaa ataaaaaata tattgtattt ttaggaggac
     5041 attttaaaat ggcaaaagaa aaatttgacc gttctaaacc acacgttaac attggtacta
     5101 tcggacacgt tgaccatggt aaaactacat taacagctgc aatcacaact gtactatcta
     5161 agaaaaacgg cggccaagct atggcttacg atcaaatcga tggtgctcca gaagaacgcg
     5221 aacgcggaat cacaatctca acagctcacg ttgagtatga aacagataca cgccactacg
     5281 ctcacgttga ctgcccagga cacgcggatt acgttaaaaa catgatcact ggtgctgctc
     5341 aaatggacgg agctatcttg gtagtttctg ctgctgacgg cccaatgcct caaactcgtg
     5401 aacacatcct attgtctcgt caagttggtg ttccttacat cgttgtattc ttgaacaaag
     5461 tagacatggt tgatgacgaa gaattactag aattagttga aatggaagtt cgtgacctat
     5521 taacagaata cgaattccct ggtgacgatg ttcctgtagt tgctggatca gctttgaaag
     5581 ctctagaagg cgacgcttca tacgaagaaa aaattcttga attaatggct gcagttgacg
     5641 aatacatccc aactccagaa cgtgacaacg acaaaccatt catgatgcca gttgaagacg
     5701 tgttctcaat tactggacgt ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc
     5761 gcgttggtga cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa acaacagtta
     5821 ctggtgttga aatgttccgt aaattgttag actacgctga agctggagac aacattggtg
     5881 ctttactacg tggtgttgca cgtgaagaca tccaacgtgg acaagtttta gctaaaccag
     5941 gtacaatcac acctcataca aaattctctg cagaagtata cgtgttgaca aaagaagaag
     6001 gtggacgtca tactccattc ttcactaact accgtccaca attctacttc cgtacaactg
     6061 acgtaacagg tgttgttgaa ttaccagaag gaactgaaat ggttatgcct ggcgacaacg
     6121 taacaatgga agttgaatta atccacccaa tcgctatcga aaacggtact aaattctcaa
     6181 tccgtgaagg cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa gcttaattca
     6241 cttaagtttt aaagtaaagc aacatttatt cggacgtaat cgctcggaac gaaggtctta
     6301 ggatctttgt tccgagcttt tttatttatt gcaggatcag gaaaaaagac aggagtatta
     6361 taaatagaag aaacacgaaa taaaagagtc agtattttct aaatgaaact ttatgaaaac
     6421 aaaatgtttt ttacaagaaa aagccttgct ttgtgagttt atatatatta taatgacaaa
     6481 ggtgcgttta ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa
     6541 cgagaggttg cgacacgccc ggacgctttg ccacgaacga gcgtgacgga aattttcgtg
     6601 gagctatgtc tacttttcaa aataggcgaa ggagggaaca agatggcaaa acaaaaaatt
     6661 cgtatccgtt taaaagcgta tgaacaccgt attttagatc aatcagcgga taaaatcgtg
     6721 gaaactgcaa aaagaactgg agctagcgtg tctggtccaa ttccattacc aactgaacgc
     6781 agtctttaca caatcatccg tgcgactcat aaatacaaag attcacgcga acaattcgaa
     6841 atgcgtacac acaaacgtct tatcgacatt gtgaacccaa caccaaaaac tgttgatgct
     6901 ttgatgaagc tagacttacc atctggtgta aacatcgaaa tcaaattata aaactaaaac
     6961 aaaaataatg gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca
     7021 caaatcttca ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc tactccaaac
     7081 gtagtactac aagtgaaaac aatggaaacg gatggctacg aagctattca agttggttac
     7141 caagatatgc gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac
     7201 acggctccta agcgcttcat tcgtgaattc aaaaatgttg agctaggaga atatgaagta
     7261 ggaaaagaaa ttacagtaga tgttttccaa gcaggagaca ttattgatgt aacaggtact
     7321 acgaaaggta aaggattcca aggggttatc aaacgccacg gacaaagccg cggaccaatg
     7381 gctcacggtt ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt
     7441 gtatttaaaa acaaacgtct tgctggccgt atgggtggca accgcgtaac tatccaaaac
     7501 ttggaagttg tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg taatgttcct
     7561 ggagctaaaa aatctttaat tactatcaaa tcagctgtga aagctaaata attgtaggaa
     7621 gaaaggagga actaaggaat gccgaatgta gcattattca aacaagatgg aagccaaaac
     7681 ggtgaaatca cacttaacga agaaatcttc ggaattgaac ctaatgaaag tgttgtttac
     7741 gatgcaatcg taatgcaacg tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt
     7801 agcgctgttc gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt
     7861 caaggatcta tccgctcacc acaatggcgt ggaggtggcg tagtctttgg accaacacca
     7921 cgttcttaca gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg
     7981 tcagaaaaag ttgctgaaaa caacttggta gcaatcgaag gcttgaactt cgacgcacca
     8041 aaaacaaaag aattcaaaca agttcttgct aacttatcta tcgactctaa agtattggtt
     8101 gttctagaaa aaggtaacga ctttgctgct ttatcagcac gtaacttacc aaacgtttct
     8161 gtagtaactt ctgataatgt gagtgtgcta gacgttgtat caaatacaaa agtgttagca
     8221 acacaaactg ctcttactca aattgaggag gtgcttgcat aatgaactta ctagacgtaa
     8281 tcaaacgccc agtgatcact gaaaaatcaa tgcttgccat ggatgacaag aaatacactt
     8341 ttgaagtgga cactcgcgca aacaaaactt tagtaaaaca agcggtagaa gctgcttttg
     8401 acgttaaagt caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca
     8461 aatatgcagg atatacaaaa aaacgtcgca aagcgatcgt tacattgact gaagattcaa
     8521 aagaaatcca attattcgaa gctgctgaat aagcactaat agataactca ttaaattagg
     8581 agggaaaaac gtggcgatta aaaagtacaa acctacctca aatggtcgtc gtaacatgac
     8641 aggttctgat tttgctgaaa tcacaacatc aacacctgaa aaatcattgt tacaaccatt
     8701 gaaaaaccat gctggacgta acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg
     8761 acacaaacgc caatatcgcg tgatcgactt caaacgtaac aaagataacg ttgttgcaac
     8821 tgttcaaacg atcgaatatg atccaaaccg ttcagctaac atcgcgttag tacactatga
     8881 agatggagtg aaagcttaca tcttagcacc taaaggacta gaagtgggta tgaaacttgt
     8941 ttcaggtcca gaagcagata tcaaagtcgg aaatgctctt ccattagaaa acattccagt
     9001 tggtactgtt atccacaaca ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc
     9061 tgctggtaca agtgctcaag tacttggtaa agaaggcaaa tacgtattga tccgcttgaa
     9121 ctctggtgaa gttcgtatga tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa
     9181 cgaacaacac gaattaatca acatcggtaa agctggacgc tctcgttgga tgcgtaaacg
     9241 cccaactgta cgtggtagcg taatgaaccc taatgatcac ccacacggtg gtggtgaagg
     9301 taaagctcct atcggacgta aagcaccagt ttctccttgg ggtcaaccag ctcttggtta
     9361 caaaactcgt aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa
     9421 ataattactt tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt
     9481 aaagaaagga ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc aagcaggggt
     9541 tgaaaagaaa aaagttatca aaacttggtc acgccgttca acagttttcc caagttttgt
     9601 tggatatacc atcgcagttt atgatggacg taaacatgta ccagtttaca tccaagaaga
     9661 catggtagga cacaaattag gtgaatttgc acctacaaga acttatcgtg gacatgctgc
     9721 tgacgataag aaaacgaaac gctaatttga ggggaggata aatccaaatg gcagaacaaa
     9781 tcacatcagc taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag
     9841 taattgacct tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac
     9901 caaacaaatc tgctggaatc attgaaaaag tattgatgtc agcagttgcg aatgcagaaa
     9961 ataactttga cttagatgtt gaaaacttgg tagtaagcga agcttttgtt aacgaaggac
    10021 ctacaatgaa acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta
    10081 caagtcatat cacggtagtc gtatcagaaa aataaggagg gataatcagt gggtcaaaaa
    10141 gtacatccaa ttggaatgcg tgtaggcatc atccgcgact gggatgctaa atggtatgct
    10201 gaaaaagagt atgctgagtt cttacacgaa gatttaagaa tccgtaaatt tatttcaact
    10261 aaacttgctg atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac
    10321 atttcaattc acacagctaa accaggtatg gttattggta aaggcggttc tgaagtcgaa
    10381 agcctaagaa aagaattaaa caaattaact ggtaaaagaa tccatatcaa tatcgtggaa
    10441 atcaaaaaac cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa
    10501 aaccgtgttg ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc
    10561 gctcaaggga tcaaaactca agtatctggt cgtctaaacg gagcagatat cgctcgttca
    10621 gaaggatact cagaaggaac tgttcctctt catactttac gtgcggacat tgattacgca
    10681 tgggaagaag cagatacaac atacggaaaa ctaggagtta aagtgtggat ctatcgtgga
    10741 gaaattcttc caactaaaaa aaacactgag aaaggaggga aataatcatg ttagtaccta
    10801 aacgtgtaaa acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa
    10861 aagaagtagc attcggtgaa tacggtttgc aagctgttga ttcacattgg atcacaaacc
    10921 gccaaatcga agctgctcgt atcgcaatga ctcgttacat gaaacgtggt gggaaagtat
    10981 ggattaaaat tttccctcac aaatcttata ctgccaaagc aattggggta cgtatgggtt
    11041 ctggtaaagg ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa atcatgtttg
    11101 aaatcgcagg cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct tctcacaaat
    11161 taccaatgaa aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt
    11221 aaagaaatca gagaattaac cactgccgaa atgcttgatc aagaaaaaca attaaaagaa
    11281 gaattgttta atcttagatt ccaattagca acaggtcaat tagaaaacac tgcacgtatt
    11341 aaagaagtac gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa
    11401 ttatggaagg aggccattaa gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa
    11461 ggtcgcgtgg tttcagacaa aatggataaa acaattaccg ttgctgtcga aacaaagaaa
    11521 aaccacccta tctatggcaa acgcatgaac tattctaaga aatacaaagt gcatgatgaa
    11581 aacaacacag caaaagttgg cgacatcgtg agaattatgg aaactcgtcc attatcagct
    11641 acaaaacgtt tccgtttgtt ggaagtagtc gaagaagcag ttattatcta ataacacgag
    11701 attttcctat atagattgaa tgcaaaatct gaaaggagga tacacaacgt gatccaagca
    11761 gaaagtcgtt taaaagtcgc tgataactca ggcgcacgtg aaattttgac gatcaaagtc
    11821 cttggtggat ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc tacggttaaa
    11881 caagcaacgc caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga
    11941 acaaaatcag gagctcgtcg tacagacggt tcatatatta aatttgatga aaatgctgca
    12001 gtgattattc gtgatgataa aagccctcgt ggaacacgta tctttgggcc agttgcacgt
    12061 gaactacgtg aaaacaactt catgaagatc gtttcactag caccagaagt tttataatct
    12121 tgagacactt atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt
    12181 aaagttatca ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa
    12241 aaagataaag ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa
    12301 gctgctcctc aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg
    12361 gtggttgact ctactggtgt agctggccga gttggctaca aagaagtaga cggcaaaaaa
    12421 gtccgtgttt ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg
    12481 tcttactgaa tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc atcattgatg
    12541 gaaaaattta actatagctc tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac
    12601 atgggtgttg gtgatgcggt atcaaacgct aaaaacttag ataaagcagt tgaagaatta
    12661 actttgatca ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat cgctggattc
    12721 cgtttacgtg aaggaatgcc aattggtgca aaagttacat tgcgcggaga aagaatgtac
    12781 gaattcttag acaaattggt ttctgtttca ctaccacgtg tacgtgactt ccatggtgta
    12841 agtaaaaaag cctttgacgg tcgtggaaac tatactttag gtattaaaga acaattgatc
    12901 ttcccagaag tagattatga tttagtagat aaagtacgtg gtatggacat cgttattgta
    12961 acaacagcaa actctgatga agaatctcgt gagttgttga cacaattagg catgccattc
    13021 caaaaataat taaaggaggc gaactacgtg gctaaaaaat caatgattgc taaaaacaaa
    13081 cgtcctgcaa aattttcaac acaagcttat actcgttgcg aacgttgcgg acgtccacat
    13141 tcagtttatc gtaagttcca tctttgccgt atttgcttcc gcgaacttgc ctataaaggt
    13201 caaattcccg gcgtgaagaa agctagctgg taaaaagtaa attcgcataa gaaggaggta
    13261 aaagatcaat ggtcatgaca gatccaattg cagattttct aactcgtatc cgtaacgcaa
    13321 acatggttaa acatgagtct ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg
    13381 aaatcttgaa acgtgaaggt ttcgttcgtg atgttgaata catcgaagat gacaaacaag
    13441 gcgtgatccg tgttttccta aaatttggta aaaacggcga acgtgttatc acaaacttaa
    13501 aacgtatctc taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat
    13561 tgaacggttt aggtatcgca atcatttcga cttctgaagg tgttgtcact gataaagaag
    13621 ctcgtgaaaa aaatatcggc ggcgaagtta tcgcttacgt atggtaatca agtagaatct
    13681 acaaggaggt gtctttaagt gagccgtatc ggaaataaag tcgttgttat ccctgaagga
    13741 gtaacagtta ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg tgaattgact
    13801 cgtacttttt cagcagatat caaaatgaat atcgaaggaa acgaagtaac tttcactcgt
    13861 ccaaacgaca gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac
    13921 atggttgtag gtgtcagcga aggtttccaa aaagcgttag aactaatcgg ggttgggtac
    13981 cgtgcacaat tacaaggatc aaaacttgta ttgaacgttg gttactcaca tccagtagaa
    14041 atcacaccac cagctggagt aactgttgaa gtaccagcta acacacaagt aattgttaaa
    14101 ggtgcgaaca aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca
    14161 gaaccttata aaggtaaagg tattcgttat gttggcgaat atgtacgccg taaagaaggt
    14221 aaaactggta aataatcgtt tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat
    14281 ttggctgaag tttaccttca aaaaaataca tttttacttg tgccgcagct tctatttttc
    14341 gaaacgtatt tactgcttga aatagtagta caataaaaat aaagaggtga caattgtgat
    14401 tacaaaacca gataagaaca aaacacgtca aaagagacat cgtcgtgtac gtaacaatat
    14461 ctctggtact gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc
    14521 tcaaattatt gatgacgtag cgggtgtaac gctagcaagt gcctctacct tggataaaga
    14581 aatttcaggt ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag ttgctgaacg
    14641 tgcagctgaa aaaggcatta aagtagtagt ctttgaccgt ggtggatacc tttaccatgg
    14701 ccgtgtgcaa gctttagctg aagctgctcg cgaaaatgga ctagaatttt agaagaagga
    14761 ggaataccat tcatggttta tatcgatcca aaacatttgg aattagaaga ccgcgttgtt
    14821 gcaattaacc gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt tgctgcttta
    14881 gttgttgtcg gtgacaaaaa cggacacgta ggatttggta caggtaaagc tcaagaagtt
    14941 cctgaagcaa tccgtaaagc aattgaagat gcgaagaaaa acttagttga agtacctatg
    15001 gttggttcaa caatcccaca cgaagtaatc ggtgctttta gtggtggtag aatcctaatg
    15061 aaacctgctg tagaaggttc tggggttgct gctggtggac cagttcgtgc cgtattagaa
    15121 ttagctgggg tagcagatat cacttctaaa tctttaggct caaatacacc aatcaacgtt
    15181 gttcgcgcaa cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt
    15241 cgcggcaaat cagttgaaga attaatcgga taaggaggac aacaataatg gctgaattaa
    15301 aagttacttt aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat acagttaaag
    15361 cgttaggtct tacaaagatt aacagttcag tggttaagcc ttctaatgaa gcaattaaag
    15421 gcatgatcaa tactgtttct cacctagtgg acgttgaaga aatttaattt agaaaaatca
    15481 taactagaac attaaggagg tgccaaacct atgaaacttc atgaattaaa acctgctgaa
    15541 ggttcacgcc aagtacgtaa ccgtgttggt cgtggtactt catctggtaa cggaaaaact
    15601 gccggacgcg gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt
    15661 gaagggggac aaacaccatt gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac
    15721 cgtaaagact atgcagtcat taatcttgat gtattgaacc gctttgaaga cggagctgaa
    15781 gtaactcctg ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa
    15841 gtattagcta atggggaact aaacaagaaa ttaactgtga aagcagcaaa attctctaaa
    15901 gcagctcaag aagcaattga agcggctggt ggatcaatcg aggtgatcta atgtttagac
    15961 tattaaaaga tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt actgtatttg
    16021 tgttgtttgt atttcgccta ggtgcacata ttactgtacc aggcgtaaat gctgcttcat
    16081 tgcaaaacct ttcttctttg ccatttttca acatgttgaa cttggtaagt ggtagtgcga
    16141 tgcaaaactt ttccattttc tcaatggggg tttcaccata cattactgct tctatcgtca
    16201 ttcagcttct tcaaatggac atcgtgcctc gatttgttga atggtcgaaa caaggggaag
    16261 ttggtcgtaa aaaactgaat caagcgactc gatatctgac attagtattg gcatttatcc
    16321 aatctgtcgg tatcactgct ggattcaatc aatacgctca attaggtttc gtgaataatc
    16381 ctaacgtagc aacttacgta acgatcggtt tgattctaac agccggtacg atgtttgtta
    16441 cttggctagg agaacagatc acagaaaaag gtatcggaaa tggcgtatca atgatcattt
    16501 ttgcaggtat catttctaga ttgcctgaaa gtatcagaga aatttatgaa gattacttta
    16561 taaatattga tccttcagac atatggaaat ctgctatttt tgtggccatt ttactagtag
    16621 cggtcttgtt gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc aagatcccga
    16681 tccaatatac gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa
    16741 atgcagctgg agttattcca gttatctttg ctagttcgtt tattgctacg ccaaatgctg
    16801 ttttacaagc attgagcggt agattcggtt ctgaaaactg gtacaaagtg atgatggaga
    16861 tctttaacta taatacagca cctggtgcta cgatttatac gattctgatc gttgccttca
    16921 ctttcttcta cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc
    16981 aaggaagtta tataccaagc gtacgccctg gtaaggggac tgaagagtat gtctcaagat
    17041 tactgatgcg tttaagtaca gttggtgcag tcttccttgg tttagtggca ttgcttccaa
    17101 tcattgctca aatggtttgg gatcttccac aatcaatcgg attaggcggt acaagcttac
    17161 tgatcgttat cggtgtagct ttagaaactg cgaaacagtt agaaggctta atgctgaaac
    17221 gtaaatatgt tggattcatt gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt
    17281 ctcacacatt tattaggagg gaaaacaatg aacctcattt taatggggct gccaggcgca
    17341 ggtaaaggaa cacaagctga aaaaatcatt gatgcttatg gtattccgca catctcaaca
    17401 ggagatatgt tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga agcaaagtct
    17461 tatatggata aaggtgcatt agttcctgat gaagtaacaa atggaatcgt gaaagagcga
    17521 ctagctgaac cagatacaga gaaaggcttc ttattagatg gctttccacg tacgctagat
    17581 caagcaaaag ctctggacgc aatgctaaaa gatttgaata aaaaaattga tgctgtcatc
    17641 gatatccatg ttggtgaaga aatcttggtc gaacgtctag ccggccgatt tatctgcagc
    17701 aattgtggag caacttacca taaaattttc aaccctacaa aagttgaaga cacatgtgat
    17761 cgttgcggtg ggcatgaatt ctatcaaaga gaagatgata aacctgagac ggttaaaaat
    17821 cgtctggcta tcaacatcaa aaatagtgaa ccaatcttgg cttattataa agaacaaggt
    17881 ttgctaaaca ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa
    17941 atcattgaaa aatagcacga ttgcccgtag ccgacttgca ttatgcagtt actcttgtca
    18001 agttcaataa attatgatag aatatgtcag tccgacttct agagaatcct ctagatgatc
    18061 cattttgaga attttgaggt gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg
    18121 aaacaagtac aaggaggtac tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac
    18181 agtcgtcgaa actttgccga atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt
    18241 tcttgctact gtttcaggta aaattcgtat gcattacatc cgtatcttac ctggtgacaa
    18301 agtaacggtt gaattgtctc catacgatct taaccgtggc cgtattactt atcgctttaa
    18361 ataattgtac tccgtaaaac tacaggaggt attatcatga aagtaagacc atcagtaaaa
    18421 ccaatgtgcg aacattgtaa agtaattcgt cgtaaaggac gcgttatggt gatttgccca
    18481 gcaaatccaa aacataaaca acgtcaagga taattggagg tgctataaat atggctcgta
    18541 ttgcaggagt agatattcct cgtgataaac gtgtagtagt ttcacttact tatatctatg
    18601 gtattggtaa cactactgct aaaaagatct tagctgatgc tggtgtatct gaagatgttc
    18661 gcgttcgtga attaacgaat gaacaaacag atgctatccg tgcagaagta gataaattaa
    18721 aagttgaagg ggatcttcgt cgtgaagtga acttgaacat caaacgtttg atggaaatcg
    18781 gttcttaccg tggaatccgt catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa
    18841 acaatgcacg tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa aaataattct
    18901 taagtgaagg aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga
    18961 agaagaatat agaagcaggt attgcacata tccattctac attcaataac actatcatca
    19021 tgatcactga tactcacgga aatgcattag cgtggtcatc agctggttca ttaggtttca
    19081 aaggaagcaa aaaatcaact ccttttgccg ctcaaatggc agcagaaact gctacaaaag
    19141 cagcaatgga acacggatta aagactgttg aagtaacagt taaaggacct ggttctggac
    19201 gtgaagcagc aattcgttca ttacaagcta caggtttaga agtgactgca attcgtgacg
    19261 tgactccagt tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc
    19321 cgctcatttt gagttttatc agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg
    19381 gtaaagataa gaatgattga gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa
    19441 gattatggca agttcatcgt tgaacctctt gaaagagggt atgggactac tttagggaat
    19501 tccctacgtc gtattttatt atcttctttg ccaggtgctg ccatcactaa tattcaaatc
    19561 gatggtgtac tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt cactcaaatc
    19621 atcttgaaca ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa
    19681 atcgatatta ccggacctgc tacagtaact gctggcgata ttatcgttga tagtgatgta
    19741 gaaattctta acaaagatat gtacatctgt agtgtctctg aaggagctac gttccatgct
    19801 cgcttaacag tgaaacctgg acgtggttat gttcaagcag atgaaaataa aaaagaagat
    19861 atgccaattg gcgttcttcc agtcgattca atctacacac ctgttcgtcg tgtgaactat
    19921 caagttgaga acacacgtgt tggacaccgt gaagattttg ataaattgac gatggagatt
    19981 tggacagatg gttcaattga accgttggaa gcaatgagtt tgtctgcgaa aatcatgact
    20041 gaacatttgg atatttttgt gaacttaact gatgaagcaa aaaatgctga aatcatggtc
    20101 gaaaaagaag aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga gttagacttg
    20161 tctgttcgct cttacaattg tttgaaacgt gcaggaatca atactgtaca agaacttaca
    20221 aataaatctg agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc attagaagaa
    20281 gttaaagcaa aattgcacga cttaggttta ggtttacgta aagaagatta aaacctttta
    20341 cgaaggaggg aaaccaacgt gagttatcgt aaactaggac gcacatctag ccaacgtaaa
    20401 gcgatgttgc gtgatttaac aactgattta ttaatcaacg aacgtattgt gactactgaa
    20461 gctcgtgcga aagaagttcg ctcaactgca gaaaaaatga tcactttagg taaacgcgga
    20521 gatttgcatg cacgtcgtca agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt
    20581 gaagaaaatg aggacatcgt cattgaatca gctttacaaa aactgttcaa cgatattgca
    20641 cctcgttacg cagaacgtca aggcggatac acacgcattt tgaagacaga accaagacgc
    20701 ggtgatggtg caccaatggt tgtcttagaa cttgtctaaa atcacactaa taacatcact
    20761 ttatagatga ttcttttaga gtgttatgat gttggaagat atttttccga gtctagctca
    20821 gcgctgctcc tcaagcgaat atcgcttggg gagtaagcac tcatcataaa gtgttttttt
    20881 ataccttcaa ttacctgtca tgacagatat gtttgaaggc ataaaaaaac attttaagcg
    20941 agaaataatt gaaaattttg tctttttttg atagtgttag taggaacttt aggatgaaga
    21001 aggagtaatc atggagccaa ttattgaatt agaaaagata aattataaat accagccaga
    21061 tgatcttcgc cccgcattga aagatgtctc ctttacaatt gataaaggtg agtggatcgc
    21121 tattattgga cacaatggtt caggaaaatc gacacttgcc aaaacgatca atggattgct
    21181 tctgccagaa tcagggatcg tgaaagtggg aaatcaaata ttggatgaag aaaatatttg
    21241 gacgatccga cagatggttg gaatggtttt tcaaaatcca gacaatcagt ttgttggttc
    21301 gacagtagaa gatgacgttg cttttggtct tgaaaatcaa gggattccca gagaagaaat
    21361 gctagtgcgt gtgaaagatg cacttgagaa agttagaatg gctgagtttg cttcacgcga
    21421 gcctgctcgt ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt
    21481 acgacctgat atcattattt tagatgaagc aaccagtatg ctagatccag aaggtcgtga
    21541 agaagtgatc tctacgatca agaagatcaa agaagaaagt caattgaccg ttatatctat
    21601 cactcatgat attgatgaag cagctaatgc caatcggata ctagtgatga gacaaggcga
    21661 acttgtccgt gaaggtacac caaaagaaat tttttctgct ggtcctgaac tgatcgactt
    21721 aggtctagat ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac gtggggttga
    21781 tgtgcccagt gaatatatga cagaagaaag gatggtggat tggttatgga catccgtttt
    21841 gaacaagtag acttcactta tcagccgaat acaccatttg aacaaagagc cttatttgac
    21901 atcaatatga cgatcaaaga aaacagttat actgcattag tagggcatac cggaagcggg
    21961 aaatcaacct tacttcagca tttgaatgcg cttgttaagc caacaagcgg tacagtccat
    22021 attggagaac gagatattca gccggatacg gataataaga atttaaagcc catccgaaaa
    22081 aaagttggca tcgtctttca gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa
    22141 gacattgctt tcggtcctaa aaactttggt gtcagcgaag aagaagcact agtcctagca
    22201 aaagaaacat tagaacaagt tgggctggat gaaagctatt tggaacgttc gccatttgaa
    22261 ctttcaggag ggcaaatgcg tagagtagca atcgctggtg tgcttgccat gaggccggaa
    22321 gtacttgtat tggatgaacc aacggcagga cttgatcccc agggcagaaa agaaatgatg
    22381 gagatgtttt ggcgtcttca taaggaacag cagatcacta ttgtattagt tacgcattta
    22441 atggatgatg tagccaattt tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt
    22501 aatagcgggg agccgcaaga ggtctttcaa aacattgaat ggctaaaaga aaaacaacta
    22561 ggcgtaccta ctgccaccga gtttgcagaa gaattaatgg ctaaagggat gaactttgct
    22621 accttgcctt tgacagctga agaattagca gacgctatcg tccattatgc tggaggaaat
    22681 gcccatgatg aataaattga tatttggtcg ttatatgcct ggagattcct tgatccatcg
    22741 attggatccc cgagctaaat tattagccag tttttatttt atcggaatca tttttttagc
    22801 aaataattgg gaaacgtacc ttttgttggc atttttcaca ttactttctg tcttcttgtc
    22861 aaaagtagat ttgaaatttt ttatccgagg cattcgccca ttgatctggc taatcctctt
    22921 cactgtagct ttgcaggtct tgtttacaag tggcggagaa acttattgga gttggggtgt
    22981 tttccatatc actgaatttg gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt
    23041 gatcattttt atgtcgactc tactcacgct tactacaccg cctttggaat tatcggatgc
    23101 gattgagtac ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg aaatttcatt
    23161 gatgctgtcg attgctcttc gttttgtgcc tacattgatg gacgaaacag aaaagatcat
    23221 gaacgctcag cgcgctcgcg gtgttgattt tggtgaagga aacctagttc agaaaatgaa
    23281 agcagtcatt cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct
    23341 tgcgactgct atggaagcaa gaggctacaa aggtggggaa ggaagaacga aatatcgtgt
    23401 attgcattgg cataatagag acacgatggt tatcattgct tttgtattat taactgtgct
    23461 actgatcttt ttaagaggat aaacgttcag tgggccgcta tctattcttt tcttgctgat
    23521 ctgtttgcaa gaaaggatgc aagcagtgta tgacaagaaa tattagggtc tgtggcagac
    23581 taaggtcaca ggcctctttc ttttatctga aaagaaagaa cgaaaaaatt ttatagaaaa
    23641 atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta cgttacaaag
    23701 caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca aatgggcgta
    23761 ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg
    23821 tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta atccattttg
    23881 attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag
    23941 aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca agatatttgg
    24001 tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt ccttttcgac
    24061 gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag cgggcgcttc
    24121 ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc tcgatagagg
    24181 ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt
    24241 tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta gtaggaacgc
    24301 ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt attgcgaaaa
    24361 aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg tatgaagtga
    24421 aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa ggtatttacg
    24481 aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc tacttctgga
    24541 acaccgttta aggaagaggt cgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
    24601 cgggaaaata gctcctcata ttttgccgtt tgttgagcta ataccgaagg tcagctgatt
    24661 gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca caaagagcaa
    24721 tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga ggcgttgata
    24781 agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt
    24841 tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt ttctacgtct
    24901 tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc cataaaaggg
    24961 gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata ggacaaaaag
    25021 gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat gtttacctgc
    25081 tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg gtttcccatc
    25141 aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg aatcttcagc
    25201 ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac ctttttcttt
    25261 gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt taggctccat
    25321 gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg tctcttgagg
    25381 ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc gcgatgtacc
    25441 attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg ggaacaaact
    25501 taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt ttggctggtt
    25561 atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg tattcaactg
    25621 attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag acaatgtatt
    25681 gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat cattaacgga
    25741 caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg tatagtcgcc
    25801 gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt tagaaatgtt
    25861 atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac ggatattcaa
    25921 atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat tacgatttct
    25981 tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt ttgcagatac
    26041 tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt catttacaag
    26101 agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat tccaatcatc
    26161 aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca tgatcaataa
    26221 taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc ccacagcaaa
    26281 agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca cataggtgta
    26341 gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga ccaaaatgat
    26401 tcctaacaac agcccacgat cgtcttgttc tatcttttcc catccttttc ctgcaagcag
    26461 gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg gtgcatgcat
    26521 accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta tggcgaaaag
    26581 actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat aaaacagaca
    26641 gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt ttgtcgtgtc
    26701 ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt ctgttttcaa
    26761 cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg ctggcaatac
    26821 aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa caatcgtttt
    26881 tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag aaaatacgcc
    26941 aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga taaataaaag
    27001 tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg gaagataaaa
    27061 aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga tcaatgcgta
    27121 actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg caagaaacca
    27181 aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg catctggcat
    27241 catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat agtaagaaat
    27301 taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac tttgctttcc
    27361 aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg catcacttgc
    27421 caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa gtgctaaaat
    27481 caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa agaatgactt
    27541 taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt cagtttatca
    27601 tatccttgtt tttctctgct taaaaagagt ctagttttca caaaaaaata aaatttagaa
    27661 taaataactt cataatttgt tcgtataaca aaaataaacg caaaaaaagg gtaggaatca
    27721 aaagtcccac ccttaaaaat ttttattaaa taaaaaaccg ttcactatct gaacggtcca
    27781 agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag ttgacaccca
    27841 aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag gtttaacagt
    27901 acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct tgcgacctgt
    27961 aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga gcaatagaat
    28021 aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag tacattttca
    28081 tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat acctgaaatt
    28141 ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg ctatgataaa
    28201 atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc atggctgtaa
    28261 aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca acagttgttg
    28321 gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat caaattaaag
    28381 ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt gtccgccaag
    28441 aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg aaaatctctg
    28501 aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta ttaggcgtaa
    28561 ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac taggcaggag
    28621 actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt gtaacagaaa
    28681 taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg caagtaaatg
    28741 cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg ggaacaaata
    28801 tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca gaaaaagtag
    28861 gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga aattccggcg
    28921 ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt gtctcattaa
    28981 atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat aaagcagtaa
    29041 tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa gctggagaaa
    29101 agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta aaaggagcaa
    29161 agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc ggtgtcgtag
    29221 acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca ttgaatggag
    29281 aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat atggtcaatg
    29341 cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat tttggttact
    29401 gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca ttcgattacg
    29461 aagaattccg taactattta gatggtatag gagattcttt acttgtggtc aatgatgacg
    29521 agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat tatggacaaa
    29581 aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat gagacgattt
    29641 tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt atcccgtatg
    29701 gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt ttaggcgcta
    29761 attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt gttaaagcga
    29821 ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac atcttcatgg
    29881 cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct tcaagaacga
    29941 tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta gaagaaaaca
    30001 aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact cacgcgatca
    30061 gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc atgatcgatg
    30121 gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca ctagaacaaa
    30181 tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga acagttgaac
    30241 aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa atcgagatcc
    30301 accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt ctgtgtcaga
    30361 atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc aggtatgatc
    30421 gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt ctttagatga
    30481 tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg ccagcttggg
    30541 catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag atattcagga
    30601 aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa tcgtagtctc
    30661 gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc gtatgatgca
    30721 agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag aaaaagtcat
    30781 tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag ccttgaacgg
    30841 gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc acgtcaacaa
    30901 atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag aatacggcac
    30961 attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc ttgaccgagc
    31021 ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc aagccaaacg
    31081 acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat tgaaaaaagc
    31141 agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat tgaaacaatt
    31201 tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga atgaaatctg
    31261 tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg taggtagcgg
    31321 aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt tccaaggtgc
    31381 gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc aacagctgtt
    31441 tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa aagaacgacg
    31501 tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca cacatgcatt
    31561 gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg agcagcaccg
    31621 ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag atgtcttatt
    31681 tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg agatggatgt
    31741 atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct gggtaagaac
    31801 acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag gacatcaaat
    31861 gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa atgcagtgga
    31921 aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct tgttacatgg
    31981 taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca accagatgca
    32041 gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg cgaccgtcat
    32101 gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc gtggacgtgt
    32161 tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa atgaactagg
    32221 tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa gcgaaaaaga
    32281 tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc tgcctcaatt
    32341 tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag aagaggctgc
    32401 agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt tagcgcaaag
    32461 cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc tatactttat
    32521 actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa tgggcggaga
    32581 tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt ttccagaagt
    32641 agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa atgaaaaaaa
    32701 catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag taaaagcaat
    32761 tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa acggtgaagc
    32821 agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt tgttcatcgt
    32881 aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag tagtagggaa
    32941 agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca aaccagaaca
    33001 tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag gtatcggacg
    33061 tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg aagtgacgaa
    33121 aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa acgtagaagc
    33181 tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta caggcaacgc
    33241 tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa aatctgctat
    33301 tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg gcttacgttc
    33361 tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg gcttgaaagc
    33421 accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata cgatccgcca
    33481 aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat ttgaaaaaag
    33541 cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag gtggacattc
    33601 ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta tgattcttgg
    33661 aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt atttcaaatc
    33721 attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat gatttaaaag
    33781 ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc ggtacggaaa
    33841 tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat tatatcagca
    33901 gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat gcagcttttt
    33961 atccttcttt ttttaatctt tttgacacgt aaaagtcatt tttctgataa ttctggcgat
    34021 aaac
//
LOCUS       DADXTG010000017        33862 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.017, whole genome shotgun sequence.
ACCESSION   DADXTG010000017 DADXTG010000000
VERSION     DADXTG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.017"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..507)
                     /locus_tag="IWI57_000688"
     CDS             complement(<1..507)
                     /locus_tag="IWI57_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2448854.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHK"
     gene            complement(584..1114)
                     /locus_tag="IWI57_000689"
     CDS             complement(584..1114)
                     /locus_tag="IWI57_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBC2448855.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1118..1978)
                     /gene="rlmB"
                     /locus_tag="IWI57_000690"
     CDS             complement(1118..1978)
                     /gene="rlmB"
                     /locus_tag="IWI57_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBC2448856.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(1971..2366)
                     /locus_tag="IWI57_000691"
     CDS             complement(1971..2366)
                     /locus_tag="IWI57_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBC2448857.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2363..3775)
                     /locus_tag="IWI57_000692"
     CDS             complement(2363..3775)
                     /locus_tag="IWI57_000692"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBC2448858.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3772..4308)
                     /gene="cysE"
                     /locus_tag="IWI57_000693"
     CDS             complement(3772..4308)
                     /gene="cysE"
                     /locus_tag="IWI57_000693"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBC2448859.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4394..4633)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4630..6090)
                     /locus_tag="IWI57_000694"
     CDS             complement(4630..6090)
                     /locus_tag="IWI57_000694"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBC2448860.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6260..7417)
                     /locus_tag="IWI57_000695"
     CDS             complement(6260..7417)
                     /locus_tag="IWI57_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBC2448861.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7451..8821)
                     /gene="radA"
                     /locus_tag="IWI57_000696"
     CDS             complement(7451..8821)
                     /gene="radA"
                     /locus_tag="IWI57_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBC2448862.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8884..9411)
                     /locus_tag="IWI57_000697"
     CDS             complement(8884..9411)
                     /locus_tag="IWI57_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBC2448863.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9641..10447
                     /gene="proB"
                     /locus_tag="IWI57_000698"
     CDS             9641..10447
                     /gene="proB"
                     /locus_tag="IWI57_000698"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBC2448864.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10444..11691
                     /locus_tag="IWI57_000699"
     CDS             10444..11691
                     /locus_tag="IWI57_000699"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBC2448865.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11767..12111
                     /locus_tag="IWI57_000700"
     CDS             11767..12111
                     /locus_tag="IWI57_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448866.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12228..12554)
                     /locus_tag="IWI57_000701"
     CDS             complement(12228..12554)
                     /locus_tag="IWI57_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448867.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13169..14371)
                     /locus_tag="IWI57_000702"
     CDS             complement(13169..14371)
                     /locus_tag="IWI57_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2448868.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14488..15003
                     /locus_tag="IWI57_000703"
     CDS             14488..15003
                     /locus_tag="IWI57_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBC2448869.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15202..15648)
                     /locus_tag="IWI57_000704"
     CDS             complement(15202..15648)
                     /locus_tag="IWI57_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBC2448870.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15695..17173)
                     /gene="msr(C)"
                     /locus_tag="IWI57_000705"
     CDS             complement(15695..17173)
                     /gene="msr(C)"
                     /locus_tag="IWI57_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBC2448871.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17527..18156)
                     /locus_tag="IWI57_000706"
     CDS             complement(17527..18156)
                     /locus_tag="IWI57_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBC2448872.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18140..18604)
                     /locus_tag="IWI57_000707"
     CDS             complement(18140..18604)
                     /locus_tag="IWI57_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2448873.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19091..20359)
                     /locus_tag="IWI57_000708"
     CDS             complement(19091..20359)
                     /locus_tag="IWI57_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBC2448874.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20590..21327)
                     /locus_tag="IWI57_000709"
     CDS             complement(20590..21327)
                     /locus_tag="IWI57_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2448875.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21394..21963)
                     /locus_tag="IWI57_000710"
     CDS             complement(21394..21963)
                     /locus_tag="IWI57_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBC2448876.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22091..22963
                     /locus_tag="IWI57_000711"
     CDS             22091..22963
                     /locus_tag="IWI57_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2448877.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23305..24177
                     /locus_tag="IWI57_000712"
     CDS             23305..24177
                     /locus_tag="IWI57_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2448878.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24174..26132
                     /locus_tag="IWI57_000713"
     CDS             24174..26132
                     /locus_tag="IWI57_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2448879.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26464..27315)
                     /locus_tag="IWI57_000714"
     CDS             complement(26464..27315)
                     /locus_tag="IWI57_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2448880.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27438..27905
                     /locus_tag="IWI57_000715"
     CDS             27438..27905
                     /locus_tag="IWI57_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBC2448881.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28168..28650)
                     /locus_tag="IWI57_000716"
     CDS             complement(28168..28650)
                     /locus_tag="IWI57_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBC2448882.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28750..29613)
                     /locus_tag="IWI57_000717"
     CDS             complement(28750..29613)
                     /locus_tag="IWI57_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBC2448883.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30211..31086
                     /locus_tag="IWI57_000718"
     CDS             30211..31086
                     /locus_tag="IWI57_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448884.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31231..31383)
                     /locus_tag="IWI57_000719"
                     /pseudo
     CDS             complement(31231..31383)
                     /locus_tag="IWI57_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31651..32982)
                     /locus_tag="IWI57_000720"
     CDS             complement(31651..32982)
                     /locus_tag="IWI57_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2448885.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(33130..33843)
                     /locus_tag="IWI57_000721"
     CDS             complement(33130..33843)
                     /locus_tag="IWI57_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2448886.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT         9886 a         7069 c         6018 g        10889 t
ORIGIN      
        1 cttgtgatcc attgctaaac attcagccat gtctacaaaa tcaacgcctt gcatataata
       61 atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg caattttcga ttgcttcttt
      121 taataaaaga tgctgaattg ctaaaggttc ttgcaatgtg atctctgcta aaaagtctgg
      181 tccttctgtc tctatttttt ggtcgattga gacttcatct ttttgatcac gacgcttcag
      241 cgcgttctgc ttcctcaaca gagaatagat ttgattgctt ataacgcttt tataataact
      301 tccaaagcta acacctttgc ttttttcgta agttctcaat gcctgatata aagcgatcct
      361 tgcttcttgt tcccaatcct cagaaacata atttcttaaa taataggtgt ttttcaaacg
      421 tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat gcttcttcgt caccattacg
      481 tgcagcgcgc aataactcct cagacataaa ataattcctc cataaattcg tttatggagg
      541 aatcttatca tcaatgattc atcgaactga ttagaacgat atgttattta ttttttctca
      601 ttaaatcgtc caatttttgg gacaattttt ttaactgctc ttcattccat ggagaattac
      661 gtcggaaatc aataaaatgc atatctttat tttcttgggc tattgttttt tccgtttttt
      721 ttatcgtctt atataattca taagcagaag ttcgtaatgc tccttgggaa aagatcatcc
      781 actgttctgc taaatcgctt gtcgccactg tcacttgtgt caaacggtca ttcagctgac
      841 cagcgattct ctcgatataa ctgtcagccg tttcgtcttc tttcgtaaaa acaacatcca
      901 actggtattt cttataattt tgttggatgc ccggaactag ttgggcatcg aagacaacga
      961 tgacttccat tccttcatat ttcgcataat ttgataaacg atgtaaaaga gcttctcttg
     1021 catccgccat tttgtcctgc tttttaagct ggacaagttc cggccacgaa ccaatcatgt
     1081 tgtagccgtc tacaatcaac agctgttttt tcatggtcta ctccccctga cgttgggaaa
     1141 aagcctgata cataagcaaa ccagcagcaa cactggcatt taagctctgg acgtgcccgg
     1201 tcatcgggat cgtcagcaat tcatccattt cttttttcaa tcctggactc attcctttgc
     1261 cttcattacc aatgactaaa gcaattgatc ctttggcatt ccatttttgg aagtttgtgc
     1321 cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt gagctgttgc acagcttgta
     1381 ccaaattcgt cacgcgtgca acagggacat gttcgactgc cccagtggaa gtctttgtta
     1441 cgataggtgt gattccaact gctcggtgtt ttggtatgat cacaccatcg acgccacttg
     1501 cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc ttcgatgcta tccaaaatca
     1561 aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat cagttggtca agggataaat
     1621 attcataagg tgtaatcgca agaacgactc cttgatgcac gccgtgatcg ctcaaagttt
     1681 ctaatttttg tttaggaacc catttgactg gcacagcttg ttcacgtgcg atttctttca
     1741 gttcttctac tttttctcct cgtgaatctt cttgaagaaa gagtttattc cctcgccctt
     1801 gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa attatcttct aatgcttctt
     1861 cattttgatc agaaactgac tgatttcgtc cagtttgctt tttatttgaa tatctatttt
     1921 gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt tgttctttct ttacccattt
     1981 ttttcaccaa ctttctgaat acaccagcgg atcaattctt ccatccgctc ggtttgtttt
     2041 gtcaaatgca gatagcccat gagcgcttcg aaccctgttg cgacccgata cgtcgtaaca
     2101 tcagcatttt ttgcgctcgt atggctttta gcatttcttc ctctgcgata ataaagttct
     2161 tcttcttctg tcaaaagatg ttcttccagc atttgctgaa tcaaaaaggc ttgtgcattc
     2221 gcagaaacat aattcgttgc cgcacgatgc agcttattgg gttttgtttc tccttgatca
     2281 atcaggtggt cacgaacata gatttcgtaa atcgcatctc caacataagc caaggctaaa
     2341 ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca tcgggttcct tgcggtgtat
     2401 cttctaataa gatccctttt tctttcaaca aatcacgaat ttcgtcgctt cggacaaaat
     2461 cacgattgct tcttgcttga tttcgttctt cgatcaattg ttcaatctct tcatctaata
     2521 actcttctgc tgtaaataaa ataccgaaga tcgaaagcca ttgcgagaat aaagcttctc
     2581 cttgtgccaa gacagcggca gaaactttcg gctggtctag ataagtgttc agccacttag
     2641 caagctcata gaccactgtg atcccatttg ctgcattaaa atcatcgtcc atttcttcga
     2701 tgaatcgctg ttctaaattt tctaacaaag cgattttatc cgcatcatct gccaaattct
     2761 ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt attcttcagt cgttgtaaat
     2821 tggtggctgc ttctttcatc gtcgtttcac tgtagcggat cggacgacgg tattgtgtgg
     2881 tcgccatgaa gaaacgcaag acttgtggat caacgttttt gatcatttca tgtacagtga
     2941 tgaaattccc tagagattta ctcatttttt catcgtcttc gccaatcgtt acatagccat
     3001 tgtgcatcca gtaattagcg aattttttcc ctgttttcgc ttcactttgt gcgatttcat
     3061 tttcatgatg aggaaattct aaatcttgcc cgccaccatg gatatcgatc gtttctccta
     3121 aatgttttgt tgccataacg gaacattcaa tatgccaacc gggacgtcct ttgccccatg
     3181 gtgaatccca agaaatctcg ccaggttttg cttgtttcca taaagcgaaa tctaatgggt
     3241 cttctttttg ctgctgttct tcccctgtac gctgacttgc tccgacttcc agttcatcga
     3301 tggattggtc acttaatttc ccataatttt caaatttacg ggtacggtaa tacacatcac
     3361 ctgaagattc ataagcatag cctttttcaa ccaacacttg aataaatgct aaaatatctg
     3421 gcatatgatc catgactctt ggatgcagtg tagcaggttc gacattcaac gctttcgtat
     3481 cttcttcaaa tgctcggatg aaacgatcag caacttctgg tgctgtaata cctaattcgt
     3541 ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt cgatacgaaa tcgactttat
     3601 agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc aatggtgcta cgagcatttc
     3661 cgatatggat ataattatac acagtcggtc cgcagacata cataccgaca tgtccctttt
     3721 gtatagggat gaattcttct ttttttctgg tcaatgtatt gtagattttt atcattctga
     3781 tctcctcact ttttctcctt tgattcgaac gacttttgct ggtactccta ccgctgtagc
     3841 gtcgtctgga acatctttca acacaacggc acctgcaccg attttggcgt tttttccgat
     3901 cgttaccggt cctaatatct gcgcattcgc agagagcatg gctccttgtt taacagttgg
     3961 gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt acgccatgga ataaaatcac
     4021 atcgtcctcg atttctgcgg tttcgccaat gacgattccc attccatggt cgatgaacac
     4081 gccagttcca atcgttgctc ctggatggat ctcaacacca gttaggaaac gccagaattg
     4141 agcattgatc ttcgccaata aaaagagccg gtgctgatat aaaaaatgag agaaacgatg
     4201 ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc tcagctgtcg aacgtgcagc
     4261 cggatcgttt ctctttgctg catcaatcgc tcgggtaaac caactcatga ctttccactc
     4321 ctttcgtaag aattttatgt aggaaatcat cttcttataa agaaaagaag cgccttttaa
     4381 ataagtttcc ttatcaaaag acgctttccg cgtggttcca cttttcttca taatcaatga
     4441 ttatctctga cagctaacgc ctgttatacg ttcttggcta ctttgtttcc tataaaacag
     4501 tttttcctgt tttctgtgtt tcgccaagac cactcccaga ggcatttcgg caaccattca
     4561 ttgatacctt gcaccacccg gtatctctct tgtaatgatc agctgcttac ttttttctgt
     4621 tcatcgtgtt tagttcagta cttgatttaa atgtgctaat gatttttcac gaccaagcaa
     4681 ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct gatacagcta cacggattgg
     4741 catgaaaaga ttttttcctt ttacgccagt ttctttttga acagctttga tggcagcttt
     4801 gacagttgga gcatctacta cttccatttg ttctagctgt gctttgaatg cgtggagaac
     4861 agtaggaact gtttcaccag caagtacttc ttttgctgct tcattcaata caggatgttc
     4921 attgaaaaat agcgtagaca gatcaacgat ttctgcagca tagctcattt gaggttgata
     4981 caggctaacg atcttcttca cccattcgat tgtcttttca tctgggtttt ctgctacacg
     5041 tccatcagcg attagataag gcagacacat gtcagtaaat gcatctagat ccattgcttt
     5101 gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg aaagctgcgg gtgatttact
     5161 taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt tcgaagattt cgtcttcgcc
     5221 cacaggtgac cagcccaata atgcaataaa gttgaacatt gcatctggta gatagcctaa
     5281 ttcgcgatat tgttcgataa attgcaagat tgattcgtca cgtttgctta gttttttacc
     5341 tgtttcactg ttgatgatca atgtcatatg tccgaaggtt ggtggtgtcc agccaaatgc
     5401 ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg tcatctccac gtaaaacgtg
     5461 agtgattttc atcaaatgat catcgaccgc aacagcaaaa ttataagttg gcataccatc
     5521 gcgtttttgg atgacaaagt cgccaccgat attatcggat tcgaacgaaa tagcgccttt
     5581 caccatatct gtaaatgcgt attctgtatt gcgaggcaca cggaaacgta caacagattc
     5641 tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc aagttcgcac atgttcctgc
     5701 gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt tctgcttcta attcttcttc
     5761 tgtgcaatag catttgtatg ctcgattgct agcaagcagc tgatcaatca gcggttgata
     5821 gatttcttta cgttcagatt gacggtaagg gccgtattct ccaggatttt caggagattc
     5881 atcccaattc attcccaacc aagcaaggtt ttccaactgg cttttttcgc catcttcgat
     5941 attacgtttt tggtcagtat cctcaatacg aataataaag tctccatcat tgtggcgggc
     6001 aaacaaataa ttgaataatg cagttcttgc atttccaata tgcaggtgcc cagttggact
     6061 tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc ttctttcaaa ctagactttg
     6121 actaacagca aaaattccgt cagttacggc ttctcaaata tcatttattt aaagccactc
     6181 agcacaaatt ttaccttttc taaggtaaat agtaaagcca actggttgaa tttccttacc
     6241 agttggctga atttcttttt tattttgatt gtttagtctt tttctctgtc tcttttttat
     6301 tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat catgcgtcca gcagctgttt
     6361 gaagggcact tgttacaacg acttcgatac gttcattcat gtaatgctgt ccatcttcta
     6421 cgacaaccat cgtgccgtca tcaaggtagg ctactccttg ttggcgttct gtcccggctt
     6481 ttactactag aacattcatc gtttcaccag ggatgacgac tggttttacg gcatttgcca
     6541 acgcattgat attaagaaca ggtacatttt ggaattcaga gactttattc aagttatagt
     6601 cattggttac aatgacacca tctaaaagct tagccaattt gatcaatttg ctgtctactt
     6661 cactgatatc ttcaaagtct ccatcgtaca tttcgacaga tataccctct tctttttgca
     6721 aggcatttag gatatctaat ccgcgacgac cacgcacacg tttcaaacta tcccctgagt
     6781 ccgcgatgta ttgtagttcg taaagaacaa aattagggat catcaaggtt ccttctaaga
     6841 atccagtctt tgtaatatca taaatccgac cgtcaatgat cacgcttgta tccaagattt
     6901 tatatttatg gaaattttct cctgattttc gttctaacag ttgactctca acttgactag
     6961 ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac tttcttccat tcttcgattc
     7021 gtgtcgttcc cattctcaat cccagatagc ctaagatgat catcagtaaa atcggcaata
     7081 cactattgac gaatggaatt tccatattgt aaagaggggt cgagatcaca acacctaaag
     7141 tcaatccgat gatcgcaccg atcgcaccga agagaagata agtcaagctg acctcactta
     7201 gtttttgttc tacttgtttg ataatagctg agatatgttt ggccaacagc aaagataata
     7261 aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt attattcagc catccgtttg
     7321 actgttgacc gattgcctgc caagctgagg gtaaaaatga aatacccaag cttgcgccga
     7381 caatgatcat cagcaaagta atgacacgtt tttgcatcgt ttttcctccg tttcgacaat
     7441 catttattag ttagcggaac acacgcttca acgtttcagc caaagttgct actccaacta
     7501 tctcgattcc ttttggcgct tcccagttac ctaagttgtt tttaggtagg tatacttttg
     7561 tgaagccgag tttttggact tctttgactc gttgttcaat atgactgaca cgtctgattt
     7621 ctccagtcag tccaatttca ccgatgaaac attccgttgg ctgtgttcct ttttctttat
     7681 agcttgaagc aatactgaca gcgatagcta aatcaatcgc tggttcattt aattttaccc
     7741 cacctgcagc ttttaagtaa gcatcttgat tctgaagaag caacccagct cttttttcca
     7801 gcactgccat gatcagagat acccgattga aatcaagacc tgttgttgtt cgtttggcat
     7861 ttccgaacat agtaggtgtg actaatgctt ggatctctac caaaattgga cgtgtccctt
     7921 ccattgccac aacgatagca gagccagttg caccgtctag gcgttcttct aagaaaatct
     7981 gtgaaggatt agcgacttct tccaatccat gctcatgcat ttcaaaaatc ccaatctcat
     8041 tcgttgagcc aaatcggttt tttactgctc gcaaaatccg aaaactatga tgttgttcac
     8101 cttcaaaata taacaccgta tccaccatat gttctaacat tcttgggccg gcgatcgagc
     8161 cttctttggt aacatgaccg acgataaaaa tggctatccc atttgtttta gcggttttga
     8221 gtagttctgc agtggtttct cttacttggc tgacacttcc tgccacactt gtgatgtctg
     8281 gctgcgtcat cgtttggatg gaatcaatga taacataatc aggggagata tgttcgatgg
     8341 cacgactgat ttcgttcata tctgtttcag cgtatagata aaattctgtg ttgatacttc
     8401 cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc ttcgccggat acatataaga
     8461 cttttccgcc tatggctgct aattgctgag aaacttgtaa cagcaaagtg gatttcccga
     8521 tacctggatc gccaccaatc aagaccatag accctggcac cacacctccg cctaacacac
     8581 ggttcagttc ttctaatttt gttttgatcc gaggttcttt tttagggacg acgtctgcga
     8641 tttttgttgg ttttgctttt tctcctgtca aacttgtccg agttctgcgg tccgttgtat
     8701 cttgttcgat ttcttcgacc atcgtattcc attttccgca atttgggcag cgtcccaaga
     8761 acttaggcga cacatacccg caattttggc aaacaaattg tacttttgat ttttttgcca
     8821 ttgtaccgcc tccttttatc ctaatcatga gacattaaat caagcttaag tatctttaac
     8881 gttttattta ctggaagaac caaatccgcc ttgacgttga gcagtcgttt gatcttgatc
     8941 tgcttttaaa aacggcaaaa agatcccttg gccgatacgc tcgccttttt tgatcactac
     9001 atccttgaaa ccaaagttgg tgaattggaa catgatatgg ccttcatttg cctcattatt
     9061 gtaataatca ctgtcaatca cacctactcc gtttgtcaac actaaaaacc gcttcaacgg
     9121 attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct cccatgtaag ctttgattcc
     9181 agtaggcacg agggttggtt tcaattgttt ttcattttct gatacatctt ttttcaatcc
     9241 atctgcgact gtttccaacc cctttttcca gcctgctttc cagatacttg gaatcacgac
     9301 ctcttctgct gcttcaaaat catagccagc agcatgatgt gtagcgcgct gcggtagatt
     9361 gatattttga tctttatatt tagaaataac ttcaaatcct ctttttctca ccggttttcc
     9421 tccctaaata aactactcct attctaacat atccgaggct gtaaaagact ttctttacaa
     9481 attcttcaag aaattctgct atcttaaagt aaaccattaa ttcgttcttt ttccaaacga
     9541 ctgcaagttg tacaaaaaaa ctatttttta catgacttta ctcgtcaaaa caagtagaat
     9601 agaacgaaat taatgagtgg atatttgatt gaggtgtcac atgcgtaaag aaattaaaaa
     9661 tgcaaaacga attattgtca aagtaggaac aagtacattg atttatccca atggaaatat
     9721 caatcttgcg gcaatcgatc agttagcttt tgtcctttct gatcttagaa atcggggaaa
     9781 ggaagtgatc ttagtctcat caggagctgt aggcgttggt atgcataaat tacatttgtc
     9841 agaacgtccg actttgattc ctgaacagca agctgcggcg gctgttggtc aagccgaact
     9901 gatgaatatc tataatcaaa gattcagtgt atataatcaa cagacggcac agatcctcgt
     9961 gacaagagac gtggtagatt atcctgaaag tcgtaaaaat gtgaggaata cattggaaca
    10021 gcttttgatt atggacacga tccctatcgt gaacgaaaac gatactgtag cagtggatga
    10081 attagatcat ctgacaaaat ttggcgataa tgatcagctg tcagccatca tggcaaaact
    10141 tgtcgcggca gaccttttga tcgtcctttc tgatatcgac gggttttatt cagataatcc
    10201 gactacaaat cccgaggctc agatgtatcg ctatatcgat aaaatcgatg aaaagattct
    10261 ggcacaagca ggtggtgctg gtagcacatt tggaacaggc ggtatgcaga gtaaattaaa
    10321 agccgcttct aggatatttg aagcaaacag cgcaatgatt ttagcaaatg gaaaaaatcc
    10381 ggcaattatt tttgatattc ttgcaggaaa agaagtaggt actttattta aggaggactt
    10441 gaaatgactg acttgatgca attaggacaa caggcaaaag atagcgcgca acaactagca
    10501 cttatgccta caaaccgcaa aaatgagcta cttgttcaaa tggcacaaac catcaaaaag
    10561 aatcaacaag cgatcattga agcaaatcat aaagacttag aaaaagcagt cgaaaacaat
    10621 atcagtgaaa cgatgcaaga ccgcttgcag ctgacacctg agcgaatcaa tgccatggct
    10681 acagagatcg aaaaaattgc ttcgttagac gatcctattg gaaaagtaga tgagatgtgg
    10741 acgaacaccg acggattgag aatcggcaaa aagcgcgtgc cactaggcgt gatcggcatc
    10801 atttacgaat ctcgtccaaa tgtcacgaca gatgcagcta gtctagcatt caaatcaggg
    10861 aatgccgtta tccttcgcgg tggcaaagaa gcattcttct cgaatcaatt attagtccaa
    10921 ttattgcagc aagtactggt cagtgctggc gaatctcctt atgccatcca gtttgtcgat
    10981 gatacttctc atgaaactgc aggacagttg atgcgattga cagaatactt agatgtgctg
    11041 attcctcgag gcggagcaaa attaatccaa cgagtcaaag aacaggcaac ggtacctatc
    11101 attgaaacag gtacaggaaa taatcacgta tatatcgata aagaagcaca gttatctatg
    11161 gcagtgaaca tcatcgtcaa tgcaaaagct tcccgtccat ctgtctgtaa cgcggcagaa
    11221 acattgctga tccattctga gatcgcacct tttttcttgc cagctattga aaaagaactg
    11281 gtagaacacg gggtctcttt acgtgcggat gctcgtgcgt tagaatatct agagacggct
    11341 gctttagcag aagacacaga ttgggatact gaatatctag attatatcct tgctgtcaaa
    11401 gtggtcgatt ctttggatga agcaattgca catatcaatc ggcataatac taaacattca
    11461 gaaacgatcg tgacagatag ttatgcggct agtcaacgtt tcttaaatga agtggacgcg
    11521 gcggctgtct atgtcaatgc ctccactcgt tttacagacg gatcggtatt tggttttggt
    11581 gcagagattg gtatctctac tcaaaagttg catgcccgtg gtccaatggg gttaaatgaa
    11641 ttaacttcca caaaatatat catttatggc gatggacaaa tccgcgaata atgtttcgtg
    11701 tgaaacatta ttcccgcctt ttgccttttt tctgtaaagg ttctatccaa ctagaaaggt
    11761 gtcaatatgt ttgaaatttt aatgattttt tttatttacc ttatttctct gaatatcgct
    11821 gcttttctgg gagtcggtat tttatccctt ttctttcaat tcaaaaaacg aagcataggg
    11881 agtcaacggg aaaaatggtc gcagtatttt gacaaaatcg ggcaaaaagg gctggttaca
    11941 cgcttgcaca ttagttacat ggttgcatta tgcttattag cgacaatcaa ttactattct
    12001 tttttcgatc attcaatcgc ttatacgatt acgctactga ttgctgggat ttttcactta
    12061 tcttataaat atcagctgaa taaaaaccac ttgaatcgga ctttccgcta aaaaatatcc
    12121 gtacgataag tagagtgcct tgaagatcgg caaaaaactg ccggactttt tagaggctgt
    12181 gacaaagatt ttgtcacagc ctctttttcc atataaacgg cgttcgccta ccagctgcct
    12241 ggtaaatctg tttcctcttc gttaagaagc gaaaatccgt caaatgtttc gccatcataa
    12301 taacgaagca gctcatccat ggacaagttc atcgtttttt ctttttcttc ttccgctgat
    12361 tcagccagta gctgttcccg atacacctga tacataccag ctattttttg ctgcatcgct
    12421 tttctagttt caccttccgc attgattatt ggcaattctt tgatatagcc acgataaatc
    12481 aaccgatcat ctgaaaattc aatgggtaat tcaacgagtg tcaacccgac ttcctgaaaa
    12541 tattcttcca gcatctatta tccccccatt ctttttctta aatgaatttt agcttaccta
    12601 ctgaaaattt tccagtttca ttattttgag aaaaaaaggg aatgtgacaa aaatcgtgtc
    12661 acatcccctt tttccgaata aatggtgttc ttctgccaac tcctcaatca taagtcacaa
    12721 tattcaacaa acatgagaaa ctgttttttg aaattgttcc ttgtgattca gagttaaacg
    12781 acagtctcac aacctcttgg taaacggcgt tcaatcagct gaccctcggt attagctcaa
    12841 aaaacggtaa aatatgagaa gctatttttc cgttttgttt ctctaatact taggataaaa
    12901 cgctgatttt acaacctctg ttaaacggtg ttcaatcagc tgtccttcgg tattagctca
    12961 aaaaacggca aaatatgagg aactattttt ccgtttgttt ctctaatact caggacaaaa
    13021 cgctgatttt acaacctcct gataaacggt gttcaaaagc ttgcatctgc ggtaataagc
    13081 ccaaccaaac gaaaaatatg agaagctatt tttgtttggc tgttcttatg acttgatgca
    13141 ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca aaataattac
    13201 ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa taaaacgcat
    13261 agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa gctccaccga
    13321 aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct ggtatttcca
    13381 tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct aacacaatgc
    13441 tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa caaataatga
    13501 ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca agagccggtg
    13561 caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca aaaccgactt
    13621 ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta aagaatccga
    13681 aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt aacgctttga
    13741 caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa agtaccgcta
    13801 gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa cgccatgaac
    13861 cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca agtgctgctt
    13921 catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc gttgctaaag
    13981 cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca atcagcattc
    14041 cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac aatcctgtca
    14101 tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc atcaccacac
    14161 cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta gcgtttaact
    14221 gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa gcgatcatac
    14281 aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt gtttgttctt
    14341 gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca tcatacatga
    14401 tttattttac atgtcaatat gcacatgtaa aacgtggaaa ctttactgtg atataataaa
    14461 gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt attatgcgtg
    14521 cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat tttttgggaa
    14581 gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg ttatgtgacc
    14641 gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga tgaaggacgt
    14701 gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa agatgaattt
    14761 ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg actgctattc
    14821 actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt gaatgacctc
    14881 tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag aattatgctt
    14941 tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca gttttttgag
    15001 taagggataa acagaacctt tctagaaata tctattgaag agcaaattat cttcaataaa
    15061 agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac ctatcgttcg
    15121 gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg ccaaggcctt
    15181 ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca cacttcttga
    15241 aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca ttttccaagc
    15301 attcatataa gcagagaata attgatcagg tattccttgt tccatgatca caccgaataa
    15361 agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga tcatttggac
    15421 cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca caccgatgat
    15481 gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca acaaacttga
    15541 taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga ccatcaaaaa
    15601 acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc tgtaccactc
    15661 ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta agtacttttt
    15721 ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca aagtaagaat
    15781 catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct aaagcttcga
    15841 tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta cttggcttag
    15901 taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta cataacggtt
    15961 tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca agcgattctg
    16021 aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc atttgataac
    16081 ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct tctccttgtt
    16141 tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca atcgcgattt
    16201 ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca cttctttcca
    16261 attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat tttggtactg
    16321 gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt tggagtcttc
    16381 tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt tgtttagagg
    16441 aagacaaccg atccagtctg atttgttgct ttttcttttt actggacact ttttccaact
    16501 tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc tctttcatga
    16561 tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg agataggcac
    16621 tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt ttcgatgcta
    16681 gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa taataacgaa
    16741 gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc tcgtctagaa
    16801 gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt tcttcccctc
    16861 cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg gctaattcca
    16921 aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg aaggtgatct
    16981 ctgtttggat tttcccatga tcgggaaaaa gttcaccggc tattagtttg agcaaagtcg
    17041 atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag acacgaagag
    17101 aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt atatttactg
    17161 ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta tcgatactaa
    17221 aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct actctgaacg
    17281 aaataactat gactaaatca ccaaatctgt catagctttc acctattcta ctgtcgttcg
    17341 aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt gaatttattt
    17401 agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt ccgacctcct
    17461 tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat agtaactgaa
    17521 aaccttttat tcgaacatag tgatcttttc tggtgctaaa cgaacagact cgtaaccttc
    17581 ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt tatccaaacc
    17641 aaatgcctct gccaactgat ctgcttcgaa tccaccaatt ggatttgttt catagccatg
    17701 atcacgcgca actaacatga attgcatagc cgctaagcta gaatcgactt tgactacatc
    17761 gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta attgttgatc
    17821 tctgacttct tttggcatct tccctgattc gtaagcttgg ttgtagattt cttcgccata
    17881 ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat cattttgtct
    17941 cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt cagcgaccac
    18001 aaagcgccat ggtttcatat tgacagaaga aggtgcagtc gtcgcttctt tgatcatttc
    18061 cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg atcttctacc
    18121 agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct ttcgtttctg
    18181 ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat tgtttcattt
    18241 ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc atttcacaat
    18301 gcttgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg ttgttctgag
    18361 gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga cgagtaactg
    18421 ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga ttttcttctt
    18481 tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct tcaaagatct
    18541 ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc tcttctaaag
    18601 acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca ataatagaat
    18661 aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct caatagaaaa
    18721 gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga aagcacaacg
    18781 ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct gcagtagata
    18841 attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc gaatattaga
    18901 aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt gtcacaacct
    18961 tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa ccaaacaaaa
    19021 aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc agctttttca
    19081 ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc atcggtgtta
    19141 gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt tcatttggat
    19201 taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct tcataacgcc
    19261 aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga tggtctccac
    19321 tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc caagcaatcc
    19381 cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc aatgtatata
    19441 aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa gttggtgctg
    19501 tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa ccataattag
    19561 gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag ttcgtattgc
    19621 ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc ttcaataccg
    19681 ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg gcattcaccg
    19741 taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg ccattatagc
    19801 ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg ttcccccagc
    19861 cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg gcacttgcca
    19921 aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta gcagatgagc
    19981 gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct tgatgggtat
    20041 gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc tctggattgt
    20101 tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt tcagttgttg
    20161 gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta ctttctgtat
    20221 cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt tctgtcgatt
    20281 cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata acgctaaata
    20341 ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc ctaaataaca
    20401 gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt tgtttctttt
    20461 taataaattt gctttttctg cataaaaaga cgtaaatgaa gaatattctt ctccattcac
    20521 gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctctct ggatattatc
    20581 cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttgctga cagacatccg
    20641 gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcagatg ttaatcccca
    20701 ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg tttgatataa
    20761 ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt gccgttgctc
    20821 ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat aaacggcgga
    20881 atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc cagatttttg
    20941 tttttccatg attggaagta cggcggaaat tccatttaac acacccatga tgttgatatc
    21001 aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg gcatgattcc
    21061 tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat cgataactgc
    21121 ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag cttctggtaa
    21181 agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta gtttagcccc
    21241 tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc ccatgatgac
    21301 aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa aatctcttgc
    21361 ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga caggtttgac
    21421 gattttggca gggattcctg caacgatggt attgtctggg acatctttcg taacagttgc
    21481 attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag ttgctcctgc
    21541 tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc ttcgttcttt
    21601 tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca atactgaatc
    21661 ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa agacattttt
    21721 ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag tcaccgtatc
    21781 atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg gttcatggta
    21841 cccagtattc agcgctgcta aaggctcttc gttttccgct tggatctgat ggatcacttc
    21901 aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga tgaggcttgt
    21961 cattttgctc cctcctttta tccttacttt ataacggacg gcttatgatg tctaatactt
    22021 ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt ttaaaaacag
    22081 gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta gagaaaaaaa
    22141 tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa aacaactgaa
    22201 agatttagaa gaagaacttg gtgtgacact tttcactcga ggctcccgag agatacagtt
    22261 gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt tggtagaaaa
    22321 aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca ttggcggagg
    22381 cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca aggcgcatcc
    22441 tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca aactggatag
    22501 cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat accagtcatt
    22561 tcgactgcca gcccaagatc gttggggact tttgatacat cggaatcatc ctttagcaaa
    22621 gcaaacagtc atccatccgc aggaactgaa aactgttcct ttgatcattt cccggcaaac
    22681 gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt tatcgatcgt
    22741 cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga cttttggtgc
    22801 attagctcta gatgggatca tcaatactta tgggacggaa aaagtattca tcccactcga
    22861 tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc tttctcctgc
    22921 tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt ttttttcctt
    22981 cgctttcagc tttttaacaa cgaataagtt aagcaaaaga tccaatgatt aaataacata
    23041 agtttcttaa tttgttcctt ccttttattt ctgataattc cagtaaatta ctataattat
    23101 aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta acaataattc
    23161 gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg gtttccctag
    23221 gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa acgtgaaaac
    23281 caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca aaatgcagta
    23341 ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg atttgacaga
    23401 aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat ggaaccagaa
    23461 ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt atttgcagca
    23521 gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga acatgtattg
    23581 atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg tatcgtcatc
    23641 cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag tattgctcaa
    23701 tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga cgaagcagga
    23761 tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca tcgcagtatc
    23821 cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt ggaaacagat
    23881 atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt acgactgctt
    23941 ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt agaaatggtc
    24001 aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt gctgaccaaa
    24061 gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca tatcgagcgt
    24121 ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa aaattgaaag
    24181 catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta cctgctgctg
    24241 ctttactagt agggatcgga aactggtggg catcttatag taacgacatt gtcgctcatt
    24301 ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt gccgttgggt
    24361 tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt ttggtcgcat
    24421 ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg aatatcaaac
    24481 cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt attattgccg
    24541 gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg atggctttat
    24601 ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg ttgctgatca
    24661 gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct tttgctaaat
    24721 tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac cgtttactga
    24781 tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta gcaggaatca
    24841 atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga atgtatcaag
    24901 ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct atttacagaa
    24961 atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gttagcagca gcttttgctt
    25021 cattctttac tggtgtaaca gaaccacttg aattctcatt tatgttcgtt gcttggccat
    25081 tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca ttcttccatt
    25141 ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct ttgaaaaatc
    25201 cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca gtgatttact
    25261 actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc cgcgaggaag
    25321 cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat gcggcacaag
    25381 caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc gataactgta
    25441 ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat aaaatcaaag
    25501 ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc atcgtaggta
    25561 cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct gcaacacgac
    25621 ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtggaacg atcgaacaag
    25681 atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca gatgatgtat
    25741 tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga gaaatcttct
    25801 ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc ggaatccaaa
    25861 cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac ttaaaaggcg
    25921 aaccattcac tttatatgta aaagaaggtc aaaaagtcgc aagaggtcaa ttgattgctt
    25981 tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc gttgtattca
    26041 caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta aaagcaaatg
    26101 ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga cttttttatc
    26161 gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc gaataaaaac
    26221 ggcgttctcc tgccaactcc gcggtaataa gtccagccaa acgtaacata tgaagagcta
    26281 ttttttattt gactcttctt attacttgat gcagaacagc ttttttacaa cctctgataa
    26341 acggtgttca aaaaccagtt tatcggtatg aagtcaaatt tatgcaaaaa tgtggaaagc
    26401 cattttcaca aaatttttct tcatactcaa aactaatcgg ctttttcaca acctctttat
    26461 acattattcc tgatccaata tttcttttaa tgctgcttca agagaagtta atggttttcc
    26521 caatactttt tctaaatcat cagaggaaat agctaactga tcctttttaa tatcatgttg
    26581 gatcgctaaa aacatgtcag ctgcttcttg agggaatccg gcttctttta gattttcgac
    26641 aaacgctgca tcacttgctt caataatttc taacttttta cctgtcgcac gctctaatgc
    26701 cttggctaat tcttcatagt cagttagatt gccggataat tctaagattt ctggagagtc
    26761 actttctact agtacttttg ctgcagcttc tgcatattca cgtttcaatg cccaacctgt
    26821 ttttccattt cctgcagcat aaacgaattg accagttttt aaagcctgcc caatgatagg
    26881 taattcattc tctacatacc aattattccg taaaaatgta tgggaaatac ctgaatcctt
    26941 aatcattttt tcagtgaatt ggtgatctgg cgctaataca ctgatcgagt gatcggcatc
    27001 tgcaaaactt gtataagcga tataagatac cccagctgct tgtgctgcct tcactacatt
    27061 cttatgttcg gcttgacgat ttcctggaac gccagagaca aaaagcaatc gatctattcc
    27121 tttcaacgct tctttcattg agtctaaatc atcataatca ccaatacgta tattaaatcc
    27181 agcttctttc agtgcttctc ctttttcttc actacgtact agtacataga tttcattttg
    27241 tggaaccatc ttttttaaga attccagcgc ataaccacca aaacctccag ttgctcctgt
    27301 tactaaataa tgcatatttg ccactcccta ttctttttat ttgttaagat ttacagctgt
    27361 acatttataa attacacctt tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa
    27421 tgaaaggatg acaactgatg aagtattctc tgcaatttag tgatgcaatc catgtacttg
    27481 cctatattga aatgtttcaa gatacccgat cactttctag tgagatgatt gccagtagta
    27541 tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga
    27601 tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa aagtccagat cagatttctt
    27661 tactagatgt ctatcgcagc atcgaaggta atacacagct tatccaagtc gatccaaaaa
    27721 caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc tttagaagaa agctacgctc
    27781 ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac acttgatagt ctgatacata
    27841 aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac aggcctcgta gagagatttt
    27901 tataattctc tctactgtgc ctgcattttt ctgatcggaa tcgtctacat aaattctttt
    27961 attctccggg ggacaaaagc cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa
    28021 gcgctgaagt catgacgtct tgataaacgg tgttcaagta gctgaccctc ggcattagtt
    28081 ctgaaaaatg caaaatatgg aaagctattt cccatttttc ctctctaatg ctcaggtcaa
    28141 agcgctactt tcacgacctc tatattatca tggtttattg actaccggta tccagatttc
    28201 tcccatatat ttcccgttgg taactcccat ttcgactaca gcatttggac ccccaacata
    28261 ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc gtattcgtca aatcatttct
    28321 taattcttca ggcgattctg ctaaagcgat tacaacaaga tacgttcctt ttgggaatgg
    28381 gatgattcgg gtagtgtttg gaacatcctg actcgtttga acaccgacat agtacatcat
    28441 tttcccgtta tagggttcgt tgactgtaaa gaaataatga ttttcagcag cttgtttcaa
    28501 tgcatctatt gttccattac cgataacttt ttgctcaaat gcttgtttct ctgcagccaa
    28561 accgacaaaa tccgtattgc tactcttcaa ttccacacca atacccgtca ccgtaaaatc
    28621 tggtttttct tcgatcctat actttgccat ttctcccacc ctttctatta tattaagttc
    28681 atcttacata aaattaaagg gaaatgacac gattacgctc ttttttctag ggatattttt
    28741 attttttaac tatttttttg ctttcactgc aatccaaatc tcactgtaaa cattactgaa
    28801 atcctctccc cattttgtaa aagagatctc gggagcttcg actaactcat aatctgaaga
    28861 aggaagccat tctgcataga ttttccccca agtatcttgt aaagtttggg gaaatggccc
    28921 tttattagga aaaatggccc aagtgtgttc ttctatagca agcatttcta aatcagcgta
    28981 cggattttct tttgtcgtag caaagccaat catgtgcatc aaactgccct tttcttccaa
    29041 gcgggctcca tcaaatccat aagaaacgtt gattacttga tttggatata aatctcctaa
    29101 cttatgcatc tcttcccttt gagtctccgt gatcgtctgc gctaactttt gtatttcttg
    29161 attgactcct tcaaatttga caggaacttt tttcgccaca cctgccacat aaaactgttc
    29221 tttcttttcg atacgaaatt ccatcgattt tcctcctctg acatcaataa aaaatgtcaa
    29281 gcgtggaaaa gatttttgta tgccgtatct gctgatttct gaaggcagat atcctgacca
    29341 atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat ccatatctaa acgccgtttc
    29401 tgtcactctt gatccatcta ggagatcgcg attagccaaa gccaatctac ggtttttgat
    29461 atagtccatt agcgacatcc ctgcaataaa cgtaaagatg cgttttaatt gatattcaga
    29521 gattcctgta ttttcggcta ttattttata ggaaagtcct tctgtcaaat gttcttcgat
    29581 gtaatcgatc aattggttca gatctttaag catatttatc ttcctccttg cttgcctttt
    29641 tatcatatta tcaaagaaag tcaattactt gatgaatcag gaaccaattt agtcagatgt
    29701 agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg
    29761 tattatctgc ctatctatgt tttaccttta gaaaaataaa ttttggggaa gacacattct
    29821 aagtaaacaa agcggtaaaa atatctttta ttcccttagt taatccgctt actacctact
    29881 gtacaaagca aattgttgtt caaactgctg atcagaacgc tgcttggatt tttctttgcg
    29941 atacatcata cctcttttat caattggaac aattatttat ttctaacaaa atcacttcat
    30001 ttttgtcaga taaataaaaa acaaaaaaag ttccatcttt aaaacacgct attcttttac
    30061 tttttctcac attctctaag aaaagaaaag gagaatgaat ttttgacaat tcgagcaaaa
    30121 gtgattatca aatatgtaaa atcagctact tttttcttaa acgtttcttt atactaaata
    30181 taaataagtg tttcatgaaa ggagaactag atgaataatt ggcttgagta ttttcctgaa
    30241 aacgttttgg aacgcggtta cagctatcat ctgcacggtt ttgtccgtca tttgaactat
    30301 acaagcaagt acttgtccgc aacagtttct ggcacggaag attataaggt agtgatcact
    30361 tgggatgaaa agaccaacat gacttgtgat tgtctatatg ccatagaagg aaaaaaatgc
    30421 aaacacatgg ctgctgtctt attcgcttac gaagaacgtc caataaaaaa atccaactac
    30481 tcactttccg aactctcgtc ccttgtttcc tccgctagtt cctctcttgt acgggagctt
    30541 ttaacagaga ttcttataga acatccgcaa tttatcgaac gcttcaaagt taagatgcca
    30601 tttcatgcaa taaattattc agataaatta acaactatca tccataaata cgatcatatt
    30661 atcaagaaaa acaaaaacag gaaaacagcg aagttcatca tggagatgcg aaagttcatc
    30721 caagaagcag ttgaatcgct cattcagcaa aatgcctatc ttcccgcatt cgaattgatc
    30781 aatgaagtga ttgctactct cgaaacattc tattgggagc ctgaggatga acgaaccctt
    30841 cttctgatag aggactgtta ttatttatgg aaagaattat tagcagaagc acctcatgca
    30901 gaaaaaaggc aaatgttctc ttggttcgtc tgccaagtcg atcatactga cgcctcttat
    30961 tcaaaacgct actccataaa aatcctgaaa gaagactttc gggaaaaaga attttctaac
    31021 caaaagaaaa aaatagataa aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa
    31081 aaatagcaag taaacgataa caaccatcct aaatccacaa gaaatacagc agaccttgct
    31141 ttaaaaacac gaatcatgca aaggatgaag taaagaagac ttaacgcgta cccaatatta
    31201 gtcatttgac tattaggtac gcgttttgtt ttataagtca tgacaaattt ccttcgttgc
    31261 tgggtagatt ttccggcatt gttgatagat tttatgatac ttttctacat tttgtaagag
    31321 aggttccact gcatctgtat attggataaa attctgacaa caactttcag gtgtgtcgaa
    31381 ccactctaat ctttaaagat aaatattaaa ataacgatta ataatattaa cagtctttat
    31441 ttttcaccac caaaaaccta tctttgcata tactaaatag acgtatatac acagataggt
    31501 ttgtcttgtg aaaaataatg attatcctta ccaaaaaatg tttgtttttc tacatgttcg
    31561 tacttatcaa aaaatatgat ccttattgaa attactggct atttatattc caacgacttc
    31621 ttgtgttaat ttttgttatc tcatagtaaa ttatgtttca ttttcactta atttagttgt
    31681 cacgtgttcg tcaattcgta aaaacggtaa atatataaac acacttgcgg ctataattaa
    31741 tatttgtaaa acagctgctc gccagtctcc ggctgttgct aaaaatcccg atataactgg
    31801 tggcgtagtc caaggaattt gcactactac atgattaatt aatcccacag cagttgcaaa
    31861 atatgctact gtcaaagaaa aaatcggtgc aagcacaaag ggaataatca gactgatatt
    31921 aaatacaata ggcaatccaa aaataatagg ttcgcttata ttgaatatag cgggagttac
    31981 cgataattta gcaaagtcct tataatcttt tctacgactg aagataaata ttgcaatcaa
    32041 taaagcaatc gtatttcccg atccacccat taccgcaaag gtatctttaa aggccatatt
    32101 gataatatgt ggaatttgct ctttgtttgc ataagctgcc atattttctt gcatattctg
    32161 aatcaaaaac ggatctaaca atgaacctga tattactgct tgatggatac caaatccaaa
    32221 aaatagatta gcaatagatg taattaacaa aaatcctggt aaaccagttg taattttact
    32281 taatggctct gtaactatcg tggtaattag cttatttata tccatattaa acaattgaat
    32341 gattataaat gacaataaag caaacgaggt aatcgtaata atgattggga ttaatacatt
    32401 aaaagacttt aaaacagcgg gaggaatatt tccggatagc tgaatctgta gtttcttatt
    32461 attagaaagt ttaataaata attctgtagc cgctaatcca acaaatatac ctacaaacat
    32521 tcctgaagct cctgtatagg atataggtat cacacccgtc acttcaacag ctttatccgc
    32581 attttcagga gtaaacatca tctgcatagg tgttaataca acaaatgtcg aaatagcaac
    32641 taataccgct gcaatatgat ttgtataatt tctgcttgca cacatatgat aagatactgc
    32701 tgctacaact aataatgtag ttatgcttaa tgtaccattg gcaattgaag taccaatttg
    32761 ttgtaacgtg gtcaatgtac tagtatcgac aatttccccc atgaatcctg ttggttctaa
    32821 gatcactgaa ttaataagtg tcacaaatcc tgctaacatc ataaatggca taaatgtagc
    32881 aaacgcatct cgcaaagttc ttaaatggat ctgattcccc aacttagtcg caactatacc
    32941 aaatttttca aatacaatgt ccttcctgct acttttgccc atattttttc tcctttatgc
    33001 ttattgtaat cgattacttt ttccacccaa ataatacatc acttatcaaa aaagtaaata
    33061 cttttaatta aatatatata tcttttttag tataaaaaaa taagctctga aaaaataatt
    33121 ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca aagtcttgat ataaatgtct
    33181 cgataccgaa tactcgaaag gtttaccaga cgatagagca acaactttgt ctatctcaat
    33241 cataaattca cctggatcta tttttaatat ttttgcgtca aattcattcg cagtatctcc
    33301 tttgatacgt atgtgcgaag tctttatttc cagacccagt tcatttgtga tataagcata
    33361 tacagatttc tttaaatgtt ttggttccaa gcctggtatg actgctaatg gcatgaatgt
    33421 ttgctcaatc gagtatggtt gacaatcaat aaaacggctt cgtacaatat tatatacata
    33481 gtcttttaca ctgatactta atttatcagc tacttttttc gatggaagtt caattgagaa
    33541 agtatggatt tctgaatcta cgtgttcttc accaaccacg ctaaacaaac cttcgtttgg
    33601 accaagaact cgtgagttat caattggtgt tctgacgact tcagtcccat acccacttct
    33661 acttcgcaaa actccttcgg aaacaagtaa gtctacgcct ttctttactg ttaacttgct
    33721 tgtatgataa ataactgcca attcatctcc tgtaggaagt ttctcaccaa ttttatactc
    33781 tccatttaca atttttttct ttatatctaa gtaaatttcc atatacttcg caattttttt
    33841 caatggtgac cctccaatca aa
//
LOCUS       DADXTG010000018        32189 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.018, whole genome shotgun sequence.
ACCESSION   DADXTG010000018 DADXTG010000000
VERSION     DADXTG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.018"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(104..484)
                     /gene="yajC"
                     /locus_tag="IWI57_000722"
     CDS             complement(104..484)
                     /gene="yajC"
                     /locus_tag="IWI57_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBC2448887.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(581..1726)
                     /gene="tgt"
                     /locus_tag="IWI57_000723"
     CDS             complement(581..1726)
                     /gene="tgt"
                     /locus_tag="IWI57_000723"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBC2448888.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1944..2642
                     /locus_tag="IWI57_000724"
     CDS             1944..2642
                     /locus_tag="IWI57_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBC2448889.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2810..5449)
                     /locus_tag="IWI57_000725"
     CDS             complement(2810..5449)
                     /locus_tag="IWI57_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBC2448890.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6068..7099)
                     /gene="queA"
                     /locus_tag="IWI57_000726"
     CDS             complement(6068..7099)
                     /gene="queA"
                     /locus_tag="IWI57_000726"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBC2448891.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7292..8467
                     /locus_tag="IWI57_000727"
     CDS             7292..8467
                     /locus_tag="IWI57_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBC2448892.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8471..9112
                     /locus_tag="IWI57_000728"
     CDS             8471..9112
                     /locus_tag="IWI57_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448893.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9109..10026
                     /locus_tag="IWI57_000729"
     CDS             9109..10026
                     /locus_tag="IWI57_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2448894.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10023..10694
                     /locus_tag="IWI57_000730"
     CDS             10023..10694
                     /locus_tag="IWI57_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448895.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11235..11723)
                     /locus_tag="IWI57_000731"
     CDS             complement(11235..11723)
                     /locus_tag="IWI57_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBC2448896.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11924..12190)
                     /locus_tag="IWI57_000732"
     CDS             complement(11924..12190)
                     /locus_tag="IWI57_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBC2448897.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12190..12453)
                     /locus_tag="IWI57_000733"
     CDS             complement(12190..12453)
                     /locus_tag="IWI57_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBC2448898.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12929..13114
                     /locus_tag="IWI57_000734"
     CDS             12929..13114
                     /locus_tag="IWI57_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2448899.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13256..15079)
                     /locus_tag="IWI57_000735"
     CDS             complement(13256..15079)
                     /locus_tag="IWI57_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBC2448900.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15677..18382)
                     /locus_tag="IWI57_000736"
     CDS             complement(15677..18382)
                     /locus_tag="IWI57_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBC2448901.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18688..19635)
                     /locus_tag="IWI57_000737"
     CDS             complement(18688..19635)
                     /locus_tag="IWI57_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBC2448902.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19825..20094
                     /gene="rpsN"
                     /locus_tag="IWI57_000738"
     CDS             19825..20094
                     /gene="rpsN"
                     /locus_tag="IWI57_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBC2448903.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20270..20632)
                     /locus_tag="IWI57_000739"
     CDS             complement(20270..20632)
                     /locus_tag="IWI57_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBC2448904.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20643..21764)
                     /locus_tag="IWI57_000740"
     CDS             complement(20643..21764)
                     /locus_tag="IWI57_000740"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBC2448905.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21780..22130)
                     /locus_tag="IWI57_000741"
     CDS             complement(21780..22130)
                     /locus_tag="IWI57_000741"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBC2448906.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22260..23771)
                     /locus_tag="IWI57_000742"
     CDS             complement(22260..23771)
                     /locus_tag="IWI57_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBC2448907.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24093..25445)
                     /locus_tag="IWI57_000743"
     CDS             complement(24093..25445)
                     /locus_tag="IWI57_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBC2448908.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25598..26674)
                     /locus_tag="IWI57_000744"
     CDS             complement(25598..26674)
                     /locus_tag="IWI57_000744"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBC2448909.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26870..28615
                     /locus_tag="IWI57_000745"
     CDS             26870..28615
                     /locus_tag="IWI57_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBC2448910.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28895..29332)
                     /locus_tag="IWI57_000746"
     CDS             complement(28895..29332)
                     /locus_tag="IWI57_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBC2448911.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29316..30689)
                     /locus_tag="IWI57_000747"
     CDS             complement(29316..30689)
                     /locus_tag="IWI57_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448912.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31011..31499)
                     /locus_tag="IWI57_000748"
     CDS             complement(31011..31499)
                     /locus_tag="IWI57_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2448913.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31521..32123)
                     /locus_tag="IWI57_000749"
     CDS             complement(31521..32123)
                     /locus_tag="IWI57_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBC2448914.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9203 a         6883 c         5943 g        10160 t
ORIGIN      
        1 atattgagca atatatccca tgctcttatc tctttctttt ttgaataaaa aatgaggtag
       61 gacatctttc tgccctacct cgtctttaaa taagctttga tacttatttt tcttccttct
      121 cttctgtctc ttccagcgtt tctgttgttt cagtttttac ttcaactgtc tcagctggtt
      181 cttctgttgt ttcaacagta gtcgttgcat catttgttac gacagtccct ggtttgaccg
      241 ttttgattgc tgcacgatcg tactcaagga agatcccttc gcagtctaaa acaactgtcc
      301 gtttgtcatt gtcgatttct gaaacaactc catgtaatcc gccgatcgtt acgactgaat
      361 cacctggttt catgctatct aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac
      421 gtgacataaa aaaccacatt gctaatagcg cgataaacat cacaagcatt ggtaatgttc
      481 ccattttttt cacctcacta attctgtatc tacaaaaaac actttagcag aaaaatgctg
      541 ataacactag ttttttactt aatttttaaa agattttcct ttagaagctt tttgcgtttt
      601 ctttattgaa cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca
      661 tgattgcttg acgtacttgt ttcattagat tcaacaagaa gtataaattg tggtaagaag
      721 tcaaacgaat cccaaatgtt tcatcacatt tgatcaaatg gcggatatag gcacgtgtat
      781 agtttttaca tgtataacaa tcacatttct catcaattgg acggaaatca tgggcatatt
      841 tcgcattttt cactactaga cggccttgag aagtcataca tgtgccattt cgagcgattc
      901 gagtaggcaa gacacagtca aacatgtcta ctccacggat aacaccatcg atcaatgaat
      961 cagcagcacc gacacccatc aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga
     1021 attctaatac acgattcatt tctgatttag gttccccgac agataacccg ccgattgagt
     1081 aaccagggaa atccatcccg actagatctt tcgcgctttg acgacgaaga tcttcaaacc
     1141 ctgctccttg gatgattcca aataatcctt gacggtctgg attagcatga gctttcaatc
     1201 ctcgttctgc ccatcgagac gtacgttcta ctgatttttt cacgtagtcg taactttcat
     1261 cgaatggtgg acattcgtca aaactcatca tgatatcaga gcccaattta ttttggatgt
     1321 tgattgcttt ttctggtgat aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca
     1381 cgccttcttc ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat
     1441 ctgttaaaat cggttgatcc cagttcataa atttatgcag tccgccagct tcttctacta
     1501 agtcttcacc agggcgtagc cataaatgat atgtattact taaaataacg ccagcaccca
     1561 tttctttcaa ctcttctggg gacattgttt ttactgtagc taatgttcct actggcatga
     1621 acattggtgt ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat
     1681 gtttttcttt cttgattaaa cgatagcgaa tggcaggttg cgtcattcat ttccctactt
     1741 tctatataaa atatcttgac aactcccacc atcataatcg tttcaagtgc tttttgcaag
     1801 ttccctcggc ttccttctac tttatttaat attctttttt ttatcctttt tttatgaata
     1861 acaaaaaaaa gaaaaacatt atgacagaaa tattttttga tacatggtat attggtgtta
     1921 ttaaagaaaa ggagggaaaa atcatgcaat cttctgcaga attaaaacgc caagcaaaag
     1981 agagtctaaa aggacgttgg ggacaagctg ttttactaaa tctaatccct acattgattg
     2041 gtattgctac tattcttctt attatgattc ctattatctt gtttgccacc accatatatg
     2101 atggatcagc gagcgtctca gaagcgacaa cttcaggagg cggtagttca ggaggcggtg
     2161 gcggaattat ttccagcatc attagcgctt tgttcatgag cggtatttct tggacttatc
     2221 ttgatatttt acgtggacaa aaagatcaga tcgaaccatt caaagatgca tttcgtggat
     2281 tcaaaggttt gtttatcggc ggagtgatca gcttagctgt gctgatctcg atctttacaa
     2341 cattatgggg tttgctatta gttattcctg gaatcattaa aagctattct tattctcaaa
     2401 gctattttat ttactacgat atcgtccaag aaacaggaca aacacctaaa gtactggata
     2461 cgatcactgc aagtcgaaaa ttgatggatg gttataaagg acaacttttc tggttagatc
     2521 tttcatttat cggctggcat atcttagcat tagcaactgc tggtattggt tatctatggc
     2581 tcagcccata catttctgca acaaaagcag cattttatga acaattgcca aaagatattt
     2641 aaaagagagt gtgacttgtg accgaggggg tggcaggaga acaccgttta tcgagaggca
     2701 gtaaaagaaa cattcagccc attagcaaaa aggaggtgtg acaacattca tgtcacacct
     2761 cctttttgta tagttccagt aaaaagaaaa tcaacttaga aacagttaat cattcctctt
     2821 cttctgcttc cggtaatcga tggaacttga tcagttcatt caccgcgatt ggtacgatag
     2881 ataaaatccc taccaagaac cagtgattca aactgatgtg tactaggccg aatagttctt
     2941 gcgtaaatgg cagcaaagca acagccgttg taatagctaa tgccagtaac gccatttcta
     3001 ccaaagcttt attggtcgct aaattgactt tgaagatcga ttggctgctg cgtacattga
     3061 acacatgcag aatcgatgaa taagctaaga tcaagaatgc aacagtttga ccgacatgct
     3121 gacttgcgtc tacccatggg ctgatagtat cgacataact acctaaataa taaccaaaca
     3181 aggttacgat ggtgaagacc cctgcattta tggcaatttt ttgcaataaa cctcgagcaa
     3241 agatcccttc atttttcgga atgggtggct gatccatgat gccgtgttcc gctggctctc
     3301 ttcctaaagc aaatcctgga agtccatcag caactacatt gacaaacagt agttgcacag
     3361 ctgtaaatgg tgctccccat cctaacagca tcgcaatcaa aacaataaag atctccgaaa
     3421 tattacagct tagcaaaaag ttgatagctt tacgaatatt ctgataaacc gaacgtcctt
     3481 gactgacagc gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac
     3541 tttgcgccac atctgttcct gtgatcccca tggcacaccc aacgttactt gctttcaacg
     3601 ctggtgcatc attcacacca tccccagtca tcgctacgac tgcacccgta cgttgccaag
     3661 atttgacgat ccggatcttg tcttctggcg ttacccgagc atagacagag agagctttga
     3721 cacgcgcatc taattcttcg tctgataatt gatgcagttc tgatcctgaa agtgcttctg
     3781 acggatcatt caagatacct aattctttcg ctatggcact ggcagtcacc acatgatctc
     3841 cagtgatcat gactgttttg atgccagcct ttttcgctcg cttgattgct cctttacttt
     3901 ctggacgtgg tggatcgatc attccgatca gaccaatcaa acgtaagtct ttttccagtg
     3961 cttcagaaga aatatcttgc ggttcttcgt cataagtagc ataaccaaca gcaatcactc
     4021 tcagtgcccg tttaccaaag cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac
     4081 taaagcgcgg agcaagaaca tcgaatgctc ctttggtgat tgagatgtat ttttcgccca
     4141 tttggtgaac tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg tttactcgtg
     4201 gatatttttc ttctaattct gcctttgtat gataattctc ttcaacagcc cttacgatgg
     4261 ctacttctgt tggattacct ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat
     4321 ctgtacatag tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga
     4381 tattcgttac ttcatcttct ttgctccata cccggcggac gcgcatttca ttttgtgtca
     4441 gcgttcctgt tttgtctgag cagataacat tagcggttcc tagcgtttca actgctggca
     4501 actgtcggat gatcgcatgt ttcctcgcca tcttctgcac accgtatgca agtgtcaacg
     4561 taacaataac agtcaaggtt tccggtacag ctgcaacagc caaggaaatc gaagtcatga
     4621 acatctcaag gagtggttct ccttgcagtt caccaatcac aaatactaaa gccgcagccg
     4681 ctaaagcgat catactgatt cgtttgccta gctgattcaa tcggacctgt aaaggtgttt
     4741 tttgcatagt attttcgttc aaaagatccg cgatcttacc catttctgta gccatgccaa
     4801 tggcagtgac gatcgctttc ccacgacctg cagcaaccgt acatccttta aataccatat
     4861 taagttggtc acctaatgac tcatcttctt cagcaataaa tgctgcatct ttttcaactg
     4921 cttcactttc tccagtcaac gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac
     4981 gggcatcggc agaaatagca tcaccggact ctaaaacaag aatatcaccc actaccagtt
     5041 gctctgactc aacagtggtc actttgccgt ctcgtatgac agttgccgtt tgtttattca
     5101 tatcttgcaa ggattccagt gctttttctg cgtttccttc ttggacaatc gccaataccg
     5161 agttgatcac aacgatagcg atgatcagca gtccttcaaa taaatcccca tgttctgtag
     5221 caaatgcggt ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg
     5281 acaagcttcg aaagaatttt ttgatcatcg attctttcgg cgcctcatcg aatttattca
     5341 atccctgctt ttttcgccgt tcatccactt cttgtgaaga caacccagta tctggatctg
     5401 cttctgttac cttctttact tcttctattg tctgcttgta ttcttccatc ctattcctcc
     5461 tttaaaatct tcgtacaaaa aaaggtactt attttactat aacatccttt ttttatttac
     5521 ttcaaataaa gggcttatcg attatttgtt aagaaagcgt aaaatttaaa gtgcttatat
     5581 ttgcaaacgc taccaaagga tgctattata tagatgtaag gaaggaggat accatcaaat
     5641 agacagaaga atttctcgat agaacagtgt aagcaaacaa agttgttaga agtacaggtt
     5701 tcacccagac atgcgtgtag agtacaaacc tataaatagt gaatgcgtat ggatgtttgg
     5761 atcacgaagt atccgaaaca agaagtaatg cgatacgtaa caattgaata acgtaaacgt
     5821 ctgaaaattg aataggaaca agagatgttt caaatgttga agatgtaacg aaaacaaaat
     5881 ttttgtttaa catgaagaaa ttcaacaccg tgctttgcat gtatgatgga aagttacaat
     5941 catttctgtt tatttgaatc cttccgaggt acaagaaagc cgagcttatc gagagctcgg
     6001 ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt
     6061 cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa
     6121 tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga
     6181 ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat
     6241 agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt
     6301 tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc
     6361 catttttacg tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac
     6421 tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta
     6481 catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt
     6541 tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag
     6601 tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc
     6661 ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc
     6721 cgtgttccaa ttcttctttc actactgctt gcaatctgcc atcaccaaaa gagattcttg
     6781 tcccaacttt ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat
     6841 tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag
     6901 caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga
     6961 tttcatggaa atgtttatct tccatctctc cagttttccg atccaaaaca agtaaacgag
     7021 aatgatcgcg gtcttttaat ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa
     7081 aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca
     7141 tgtttttcgt tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa
     7201 taaaaaaact accctatttg tattttttca ttgttttagg catactagta atagatagtt
     7261 cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat
     7321 ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt
     7381 catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg
     7441 aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga
     7501 tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca
     7561 catgacgatc cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga
     7621 acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga
     7681 tcgctaccca catgaattat ctggtggtca gcagcagcgt attggagtcg ttcgtgccct
     7741 tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg atccaatcac
     7801 tcgtgattcc ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt
     7861 atttgtaacc catgacatgg atgaagcgat taaactagca agccgtatcg ccatcatgag
     7921 tgaaggccgt gtgatccaat ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga
     7981 tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag
     8041 agtcggcgaa gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga
     8101 agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa
     8161 tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aaacgaggaa aagtatccag
     8221 tgtcggcgac attttgaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga
     8281 taccttgcaa cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa
     8341 caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg
     8401 gggagaagat cccgcaacta ctgatgttgc agagtcagct actcctgaag aatctaagga
     8461 ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt
     8521 tgaacatctt tatatttcag gacttgcttt gatcattggt atcatcattg ctgtaccgct
     8581 tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc
     8641 cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg
     8701 taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac
     8761 gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat
     8821 gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc
     8881 tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg
     8941 cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat
     9001 tttggcaggt acgatccctg taacaatttt agcactattg gctgattggc ttctaggtct
     9061 cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata
     9121 ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc cttccctggt
     9181 cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca
     9241 cagatcttag caagtctagt tgccggaatg gttgaacatt atacagataa aaataccgca
     9301 atcatcaaca atctggctac tacaactatc aaccatcaag cgatgatgaa cggagatgct
     9361 tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct
     9421 gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacggt
     9481 caaacgtggt tcccgtccta tagttttgaa aatacgtatg tctttcttgt tcgaaaagac
     9541 accgcacaaa aataccatct atctaaagtc agcgatctga aaaatgtagc ggatgaatta
     9601 gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa
     9661 gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac
     9721 gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc
     9781 agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct
     9841 ccagttgtag acaacaagat attagctgat acgcctcatt taaaagaagc cttaaataag
     9901 ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat
     9961 ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg
    10021 aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa
    10081 atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc
    10141 tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag
    10201 caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct
    10261 ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt
    10321 attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca
    10381 tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt
    10441 taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg
    10501 ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag
    10561 gaaccaatgc aacagagggc acatcgatta ttttagcagg tgcattgcca actgcattaa
    10621 tggcgatcat cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc
    10681 gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca
    10741 aaggcttttt tagttctgtc atttttaagg caataatcca ctctgattct tccctcctta
    10801 tgggcaaaga gttgcttttc ttagctattt atccattccg tatactccaa cttcatactg
    10861 ccaacgggtt catatctgca tccaccgact aagttttttt cttatccgct aaggcatttt
    10921 gccaatccat tttcccatca ctcgtcatgg aattcatacc acatgatctc taaatactcg
    10981 tctgcttgtt ctttgaattg ggcatcaatt cttacatcct ttggcgtggc tttcatttaa
    11041 gccttttcca tcagtcctcg attgttcgat gccttcagct gcatcatcaa aactggtgac
    11101 tgccgtatcg gtaacttcga tcatctttta tacaatcggg aacttgccat cctcatcctt
    11161 ggctaaaaac atatctagcc attccagctt ctcgccatgg gaggtttgcg gttcctaaaa
    11221 ccggtacacg attcttatgg actgcatccg tcgcaactag acttggcgat acgatgatcc
    11281 ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat attgccaata agaaaagaca
    11341 ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt tgatactgct attcatggcc
    11401 gaaagcgcca cgactttaac ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct
    11461 ttggataccc gctctgctaa aggaacgaca ctggtattgt atttcgtatc tgtatctttt
    11521 ccaaaagacc atgccaacga atcctctggg aaccaataag acgactccgg ttgattgtcc
    11581 attgcttttt tcaacatact tttggctgtt tctcgataaa cgacatttcc aattgtcttc
    11641 gcctcacttt ggcgataatc gatggtcgtt gccactgcca acactaacaa cgatgctcca
    11701 attttactga ttttccttgt cacacttctt cctccttgtt ttacataaac ccgtcaacat
    11761 tccattccta aactcaaaca atgacccttc aaaggtggag atataggaac aaattaaaga
    11821 aatcatgcaa taaatccaag aaggttggtt ttagaacaaa aaaagagtct cctttttggg
    11881 gagactcttt gttaactatc taaaaaggtt ggctcttctc acttcactca taatgtgtcc
    11941 aggcagaata aatttctaca actttatttt cctcatcaac tttgtaaacc actcgatgct
    12001 gtctattcag cctgcgtgag tatctgcctt catgtgtagg tctcagcttt tcaaaagact
    12061 gcgtgggcat atatggatct tccttcaatg tctgaatcac ttcttcaaat tgtttcttta
    12121 aattcgtttg cttgattttt cttaaatcca ctttcgcaga gtttttgatt gcgactgtat
    12181 agttactcat caaagagctt cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc
    12241 tttttcgcgg tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg
    12301 aatcgctctc cagtcttcca gcccaatcaa tacagcattg tttccactac gatcactaat
    12361 aatctcgatt tcagtatgat tttcatttac ctctttcaat aactgataaa aatttttacg
    12421 agcagtgcta ggattcatcg ttgtattagc cattgtctct cctccttttt ctgatataat
    12481 tatagcgtac ttgattaagt acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac
    12541 ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct
    12601 cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat
    12661 catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga
    12721 aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga tacgcacgaa
    12781 agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa
    12841 tgattgcctc tttcgtctcc tgtcattcat gttcactatc gatttgatac ttgcttagac
    12901 cgttgggttt gtgaatcacc aattaaaaat ggcactgagt tatggtaaca aaaaatggtc
    12961 acttgacgag gctgagattc ctacttatga agacacatgg cacttaagag tcgaggtaaa
    13021 ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga
    13081 tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac
    13141 gctgttccca tctttaatgg cttatagagg gcacctttag ctgagaataa gacaaaacag
    13201 accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt
    13261 tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc
    13321 ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt
    13381 gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta
    13441 cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga
    13501 catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt
    13561 ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac
    13621 cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat
    13681 gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga
    13741 tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta
    13801 accgatagac aaacaaaatc gcaatgattg catatgagat gaacgcaccc gtaatattgg
    13861 caatcgattt gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt
    13921 gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg
    13981 agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg
    14041 aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca
    14101 gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca
    14161 gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa
    14221 tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg
    14281 tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat
    14341 tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta
    14401 aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag
    14461 ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac
    14521 caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg
    14581 atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag
    14641 aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg
    14701 ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca
    14761 agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat
    14821 aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag
    14881 cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga
    14941 tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag
    15001 caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca
    15061 acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat
    15121 agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata
    15181 aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt
    15241 aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg
    15301 atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact tttggaagag
    15361 agatgtaagg ctgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac
    15421 aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta
    15481 aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga gcagcttttt
    15541 tacaacctct tggtacacgg tgttcaatca gctgaccctc ggtattagct caaaaaaacg
    15601 gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca aaacactgat
    15661 ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg
    15721 tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag acaacaaaca
    15781 aaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct cctgcagatt
    15841 cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt tggaagaatt
    15901 tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt actaagatga
    15961 taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag acgatcatca
    16021 tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt tggacatcta
    16081 ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca
    16141 aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt cggtctttct
    16201 ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg acccacagac
    16261 atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga taaagcgcat
    16321 tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg tctagtttca
    16381 ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc gcgtctgctg
    16441 ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc gcagcagcgt
    16501 tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta ccggtgattt
    16561 cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct aatttttgct
    16621 gaatgatcgg taattcattg ttgtataggt ctgccccttc attgatgcca ttgacgatgg
    16681 tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca attgcaggca
    16741 tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct ttcgttgagc
    16801 tgagaagcga atcaaagtcg atctggtccg ctttgctcag tgcgccttgt gcagtcccaa
    16861 tcgtatcgat gacttggtct agtagagaag tgacagtgga actgatttga cctgtattga
    16921 taccagcaat gatgccattg atattattaa caacggcatc aatttgatct agcaattcat
    16981 caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg cggttttgca
    17041 gattgcttag caagggtttt aattgattta gctgatcaat aattggctgt aatttcgtat
    17101 ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt gcattttgtt
    17161 gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc ttttcatcat
    17221 cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa cctgtcgttg
    17281 ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg
    17341 catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt
    17401 ctaaattttt cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt
    17461 ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct
    17521 ctgctagttg cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga
    17581 tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca
    17641 tctcatctac tttagggatg taatccacga attcatttgc tttattcagt ttttccttga
    17701 tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca
    17761 aattttcatc tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat
    17821 cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa
    17881 attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa
    17941 tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag
    18001 taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc
    18061 cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt
    18121 tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact
    18181 ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat
    18241 cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga
    18301 tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa
    18361 gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt
    18421 accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat
    18481 atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc
    18541 ctttacttta tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta
    18601 tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc
    18661 ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc
    18721 aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag
    18781 attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa
    18841 ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt
    18901 ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat
    18961 gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc
    19021 ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc
    19081 taattttttt accttttcct catttttact ggatgcactg gttaagattc ggattccctt
    19141 ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac
    19201 agtatctgtc ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg
    19261 cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc
    19321 tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc
    19381 acctacatga acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt
    19441 acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc
    19501 aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac
    19561 ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat
    19621 tgcaaatctt cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat
    19681 tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt
    19741 tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata
    19801 aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa
    19861 cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat
    19921 tacgaaggct tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat
    19981 ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc
    20041 cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag
    20101 tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat
    20161 acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca
    20221 cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa
    20281 caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa
    20341 tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct
    20401 gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt
    20461 gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc
    20521 taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg
    20581 atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct
    20641 ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg
    20701 atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg
    20761 ataagcgtca cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc
    20821 atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc
    20881 atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca
    20941 ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga
    21001 atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc
    21061 ccagagggat taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca
    21121 tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt
    21181 tttttcagca cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga
    21241 tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg
    21301 aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg
    21361 tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct
    21421 gctgcttgca accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca
    21481 gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct
    21541 ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact
    21601 gctgcctcag ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac
    21661 aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt
    21721 ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc
    21781 tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat
    21841 acattgcctt catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc
    21901 tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct
    21961 ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat
    22021 agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc
    22081 cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct
    22141 cttttcaata aatttagaac actctcattc taccaaatta taaccatttt gccacaaata
    22201 agtccacaaa taacaaaaat gaaagtttct cttaaaaaag agaaactttc attttatgtt
    22261 taatcgttat ttgaacggat tacaaatccg cgttttttat cattgtgacg tttaccgcca
    22321 tctttttggc tgttattatt tttgctataa ccgccttttg atttgtttcc acgataatta
    22381 ccgccgtcac gacgattacg gttgtttccg ccaccacgtt tgccattttt attgtatcct
    22441 tttttctgca ttggcaatgg gcgttctggt gtgatcttca ctggcactgc atcagctgga
    22501 tcttttgctg tcgtttttag caaaagtgca actaaatctt gtgcagaata ttcttctaat
    22561 aatttatctg ctgtttgcag gtatttatct agaccatttt ctgcaagttt tgtttcgatt
    22621 tgttcaactg cagcacctaa ttgacctttg aatgcttctt tttcggttgg tggacgtaaa
    22681 gtcgtcatcc gttttttcgt caagttttca atcacatgca agtagtccat ttcatttggt
    22741 gtaacgaaag tcacagacat accgcctttt ccggcacggc ctgtacgtcc gatacggtga
    22801 acgtagcttt ctggatcttg agggatatca tagttgtaca catgagtgac acctgaaata
    22861 tctaatccac gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc gctcttaaat
    22921 gaacgcaata cgctcattcg tttttgttga gacaaatcgc catggattcc ttctgcacgg
    22981 taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag
    23041 acgattgtta attcaggtgt ttgaacatca aacaagcgag tcatgatatc gaatttttcg
    23101 tattcttttg cgcggacata gtattgatcg atcaagtcag ctgtcatttc tttcgctttg
    23161 atcttcacat gatgtggatt tttcatgaat ttaacgccaa tattcttgat agctggcggc
    23221 atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg
    23281 atatcttcca agaagcccat attcaacatt tcatctgctt catccaaaac taatgtttga
    23341 acggttccaa gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc
    23401 acaacgatat gtggacgatc tttcaagcca cggatctgac ggccaatatc tgctccgccg
    23461 taaacggctt gtacacgtac ttttttgtcg cgccctaaac gatataattc ttcttgtgtt
    23521 tggatagcta attcacgtgt tggtgcgata actaaacctt gtaattcgtg acgatctgga
    23581 tcgatttttt ctaacattgg taatccaaat gcagctgttt tccctgtacc tgtctgtgct
    23641 tgtccaataa cgtcttttcc agctaatgct agtggaatcg tttctgcttg gataggcgtt
    23701 gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa ttctagttct
    23761 ttaaatttca aatgaatcct cctatatggt tgttaactga ggatgtagct tttttgctac
    23821 cccgtcgatt tgttcggtca aagtgtattg tttctttttg acagtggcca cgcacgtttt
    23881 tatccattct tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt taggcattag
    23941 aaaagccaaa cgggtcgcgt tcagctttat cattattatg acatctggct tcaacaagtt
    24001 gtttctatgg caaaaccagt ccaatttctc ttcatctttc ggcaataggt tattctaaca
    24061 tagtttttcg ttttctgcaa gtttacagat ttttatttca tttgctgaag ttgttctacc
    24121 acttcaaata agcccatgct gttgctgcct tttagcataa tactatctgt tgcttcaaga
    24181 ttttgtttga tagcatcaat caatttattc ttttcttttt caaaatagaa cacaggtagt
    24241 tgggggtatt tttggttcaa ttcttctttt agtgcatgca tttgttcccc ataaaggaaa
    24301 atcatggcga atttctcagg atcgatatgt tcactcattt gcgcatgcat atttaaagac
    24361 tctttcccta attccagcat atctgctaac acagcgattc tgcgtccttt tgttggcaga
    24421 tttttaaaag tatctaatac cagtcccatg gctgttggat ttgcattgta gacatcgctc
    24481 aaaatagctg caccatttcc tgccgttagc cattcggttc tgttttgagt gatagaaaca
    24541 tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa agaagcgccc gatcgtatac
    24601 gcaatcaaag cattcgttac attataactt ccaggcagcg ggatcgtaaa tgtttctcct
    24661 tctactacaa aatcggtttg tgcttttcct tttgctgtca cttccgcatg aacatcgcca
    24721 tttccaatac cgaatgttgt gactgtttgt actaatgttg ccgttaaagg tgtcagcaac
    24781 ggttcatctg cgggcacaac gagtaatccg tctgatacaa gtccgtcagt gatttccatt
    24841 ttggctttag cgattccttc tcgcgaccca agattttcta tgtgtgcttc accgatcatt
    24901 gtgattgctg cagcttttgg ttgagccata agtgaaagtt ccgtgatttc attggcgtga
    24961 tccatgccca tttccaaaac gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa
    25021 ggaagaccaa tattattgtt gtaatttcct tgcgtcttat atgttttgta tttttcagac
    25081 aagacagacg cggttagatc tttcgtggtg gttttaccat tgcttcctgt aattgcaacg
    25141 acttctgcgc ctactttttc caagtaatag gttgccaatt gctgcatggc tttcaaagga
    25201 tctgctacag gaataactgg aaaatttttt ggagcttgtt caacaggcaa gctccaaaat
    25261 gaagcagatg ctccgtttgt gatcgcttgc tggataaatt catgtccatc tcgcgtacca
    25321 gcaagaggaa cgaataaact tccttcggtt atcttccggc tgtcaaattc gacattggta
    25381 atctttgtca gtgcagagca ttcgctgccc acggcttgtg caatttcttg tattgttaga
    25441 ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt
    25501 cgcatgactc tcacttccaa gtaaatttct ctattcgtat ttagaagcta gaatgaacgc
    25561 ggttacgcaa tcactccagc ctcttcttta tttctcttta ttcatttttt tcaaaaaaag
    25621 attgacgctg atggtatcga ttcattccta actggatcag ttcttcaatc aaatcaccgt
    25681 atttcaagcc catattttcc cataagagtg ggtacatact gaactccgta aatcctggca
    25741 tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa tcgcaccggc
    25801 tcaatccgct tccacctaac atcgtgtaag ctaacttcgc gtactcttgc gctttttgat
    25861 aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg atatattttg
    25921 cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc gtccgaacgt
    25981 cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt tcaacgattg
    26041 ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct cggttttctg
    26101 cttttgtaat gccgacacta gaacccatat tcgccggttt gacaaacatc ggataaagca
    26161 aagaaccttc acattgatca aatacttttt taggattttc tttccattga ttcttaagta
    26221 ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg gtcatgattt
    26281 tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct
    26341 ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact ggaaaaacga
    26401 tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag ccttcctctg
    26461 tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt ttttctgtta
    26521 gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga acttggtaat
    26581 aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact tcatgctctg
    26641 cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt attctaaatc
    26701 gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata acttatttat
    26761 aaaaaaacga gccagcttct aaaagaacga acgtcattta ttttttgtca gctagattat
    26821 ttgtcccgtt ttttataaaa agttatgata aagaaaagga gggatgatga tggcgcaaac
    26881 aaatccacgc agccgcttgg cagaaatcat tcgagtgttc atcaattaca atgtagtgcc
    26941 aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc
    27001 caccttcatc aagataggac agatgctttc tgttcgagaa gatttgcttt cttctgcctt
    27061 cactcaaaca ttcaaaacat tacaagatag tgtacctagc gacacatttt caacggtcaa
    27121 aaagacgatc gaaacagaac tttctctttc attatcagat atattcgatg atttttcaaa
    27181 atcgcctttt gcctctgctt ctatggggca ggctcatcgt gccaagctga agaacggtga
    27241 cgcggtagtc gttaagatcc aacaccctaa catcgccgaa gagatccgac ttgatcttca
    27301 actatttgaa cgagcgatcc ctttgatcaa atacattcct gaaactagcg tagttgatct
    27361 aaaaggggta ctccaagaag taaaacgttc cttgatcaat gaaatggact tcttaaaaga
    27421 aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga tccgcagccc
    27481 caagatttat gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa
    27541 aaacttgaat catttaatga acgccgaaaa caaaactgaa acttttatca ctggaattca
    27601 aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt gagaacttta tgaaacaggt
    27661 gtttgatgat ggttttttcc atgctgatcc tcatccaggg aatctgctat tccatgtact
    27721 aacaaaagaa gaacagacac aagctagccg aaaaactgaa acagtccatg aaaaagagtt
    27781 cggttccttt gcttttcgag cctccacttc tgcagaagac cctgttgcgc catataccat
    27841 caactacatc gattttggta tgatggggca tctttctgca ggacttaggc aaaaactcac
    27901 acaagccgtt ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag cggtccttcg
    27961 actttgtcag caagaaggct cgtttgatga aagtcggttc caccaagaac tgacaagttt
    28021 tcttgaacag tattatgatt ctcctattga tgaaatcaac ttgcaagaag tattcacaca
    28081 tgtcgtgacg atttgtcatc aaaataatct gcagttcgat cgtgatatca cgcttttgct
    28141 gaaagcattt gggacattag aaggggtgat ccgcgtcctc gatcctgaag tttccctcat
    28201 ggaagtcgct tcgccttttg ctcaacatta ttttctcact catctggatg tagaagatac
    28261 attgaaacaa agcggattag acttattgga agggatgaag gctgcgccta agatccctca
    28321 acagctccac catctattag aaatgtggac ttcgggacaa ggaaaagtca atttggaatt
    28381 gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg
    28441 tatgatttta gctgcattga tcgtagggtc ctccctgctt gtccaagcag cacctgtaga
    28501 aaacgcagag gtcgtttcct tattagggat atttacgtat gctattgccg cattcgtcat
    28561 tatttttttg gcaatcgatg cattgatcca aatgtataag aagaggaaaa agtagaaaaa
    28621 ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg gttttacata aattgtttct
    28681 caaatttttg tgaaaattcg gcttattttt cgaagaggct acttctgaaa caccgagaat
    28741 aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa gggaacatgg
    28801 aacacattta tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc
    28861 ttcgttacct actatcttct gtacttggta aaccttagtc aattgccttt tttaacgctt
    28921 gacgaaaaga catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag
    28981 cttttcttaa ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt
    29041 caaattcttt tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta
    29101 aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat
    29161 ccgcactatc agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg
    29221 tcgtataatg gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt
    29281 ttatgacatc ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc
    29341 gccttcttcc ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc
    29401 cattttgcca agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg
    29461 atcaataata gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc
    29521 aataaaaact ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg
    29581 cgatccattg gaatagctgt atcagtttga taatattcct tatttagcca gctgcctgtt
    29641 actacgtgcc agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg
    29701 accaataaag aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt
    29761 ttcaaaatcc atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac
    29821 ataaaaatcg aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaataaa
    29881 aaaaggaaat tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac
    29941 ttccattcct tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa
    30001 ccataaatag caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga
    30061 aacaataagc caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt
    30121 ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg
    30181 acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca
    30241 atcgcttgat acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata
    30301 ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat
    30361 gctgcactca gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct
    30421 aatattccaa gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa
    30481 ctatctagac caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa
    30541 aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa
    30601 agataaggaa taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta
    30661 cgcccaaatc gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta
    30721 atctgctctt cgattttagc attaaagggg aaataaacca attttacaga atatattctt
    30781 gtaaaaaaaa atacagaaag aagcataagc ttctttctgc atttttacgg tacttgatta
    30841 ctcaagcaga caagcccttc tccacaaaag tcttgcccga cacccctgca tccggcaggt
    30901 gataaagata gaaatcaatt atcctgtgcc tccaaaatca attaggagct ctgtaaacca
    30961 gagtccttct cgacccgtcg cataaattta ctttaacaca gttcctatgt ttatgcgaga
    31021 gcttttttca ttttataatc gaccatttga aatccttgac cgatatctga aataagttct
    31081 tctgtaatct caaatccttc atgcttataa acctcgattg cttgatgatt cccctgattc
    31141 actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa
    31201 actgaagaag tcaatccttg accacgtact ttatttagca aatataattt acttaaataa
    31261 atttttccat ttatctcttc ataagcagtg taaccgacag tcttttcttc gctttttaat
    31321 aaaaagtagg acacaccttc tgtcagttct ttttgaatct gtttttttga ctgataagtt
    31381 ttcagcatgt aatccacttg tttttgacca atgatcggca caaaaacttc cggccatact
    31441 tcttgtatga ctgccatcag ctcatccagt tcattttctg ttacttttat tttttccata
    31501 cacccacgtc cttttttaca ttataaatga ccttgcgctt gatggaataa aaagagcatg
    31561 atcatgctat acacacccac cgaccacggt ttcaagaaaa tcacaatctg cataaattcc
    31621 ctaaaagata atttcgtcaa accagctact agacagatca agtcgtctgg cgcaaaggga
    31681 agcagcaagg taacaactag tactttttta atatctttcg tttttcttgt cagcaattca
    31741 tcaaatttct gatacttttt tggcgataaa ataagctgga caaaggctac gccatagtaa
    31801 cggacaagca aaaatccgat tgcttcccca atcactaggc caatacaact atataaaagc
    31861 ccaaatccat tgccaaacat cagcatgccc gctactgagg agatccctcc cggcaaaata
    31921 ggaatgatca cttggatcac ttgcaacaga acaaatatca tcactgcata cttgccaaat
    31981 tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg ataagcataa
    32041 agaatcaaca caaagaaaaa aaacagacca ataatgggca tgatctgaag acatttttgt
    32101 aaaactgatc cttttttaga catctatatt ccccacatct tttttcattt attaaaactg
    32161 cctccttatg atgtgcaccc caaaagtta
//
LOCUS       DADXTG010000019        31613 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.019, whole genome shotgun sequence.
ACCESSION   DADXTG010000019 DADXTG010000000
VERSION     DADXTG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.019"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..255
                     /locus_tag="IWI57_000750"
     CDS             <1..255
                     /locus_tag="IWI57_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBC2448915.1"
                     /translation="FRRGGNCKGLDVLALDEDDLYDQKLTDNPIGFGLLPEDDEGNEW
                     FKMTLTDDNGDELSVEDTWSYLSDYIVSVEIIEFVADKEE"
     gene            369..608
                     /locus_tag="IWI57_000751"
     CDS             369..608
                     /locus_tag="IWI57_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448916.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            675..914
                     /locus_tag="IWI57_000752"
     CDS             675..914
                     /locus_tag="IWI57_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448917.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            1041..1730
                     /locus_tag="IWI57_000753"
     CDS             1041..1730
                     /locus_tag="IWI57_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBC2448918.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1736..2902
                     /locus_tag="IWI57_000754"
     CDS             1736..2902
                     /locus_tag="IWI57_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBC2448919.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            2984..3655
                     /locus_tag="IWI57_000755"
     CDS             2984..3655
                     /locus_tag="IWI57_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBC2448920.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3708..5684
                     /locus_tag="IWI57_000756"
     CDS             3708..5684
                     /locus_tag="IWI57_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBC2448921.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5697..6449
                     /locus_tag="IWI57_000757"
     CDS             5697..6449
                     /locus_tag="IWI57_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2448922.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            6465..7226
                     /locus_tag="IWI57_000758"
     CDS             6465..7226
                     /locus_tag="IWI57_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448923.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            7239..7499
                     /locus_tag="IWI57_000759"
     CDS             7239..7499
                     /locus_tag="IWI57_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448924.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            7496..9586
                     /locus_tag="IWI57_000760"
     CDS             7496..9586
                     /locus_tag="IWI57_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBC2448925.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(9622..11679)
                     /locus_tag="IWI57_000761"
     CDS             complement(9622..11679)
                     /locus_tag="IWI57_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBC2448926.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(11824..12015)
                     /locus_tag="IWI57_000762"
     CDS             complement(11824..12015)
                     /locus_tag="IWI57_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448927.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            12203..12658
                     /locus_tag="IWI57_000763"
     CDS             12203..12658
                     /locus_tag="IWI57_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448928.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            12655..12897
                     /locus_tag="IWI57_000764"
     CDS             12655..12897
                     /locus_tag="IWI57_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBC2448929.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            12915..13217
                     /locus_tag="IWI57_000765"
     CDS             12915..13217
                     /locus_tag="IWI57_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448930.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            13286..15317
                     /locus_tag="IWI57_000766"
                     /pseudo
     CDS             13286..15317
                     /locus_tag="IWI57_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15317..17929
                     /locus_tag="IWI57_000767"
     CDS             15317..17929
                     /locus_tag="IWI57_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBC2448931.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            17926..20304
                     /locus_tag="IWI57_000768"
     CDS             17926..20304
                     /locus_tag="IWI57_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448932.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            20365..20907
                     /locus_tag="IWI57_000769"
     CDS             20365..20907
                     /locus_tag="IWI57_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448933.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            20985..21317
                     /locus_tag="IWI57_000770"
     CDS             20985..21317
                     /locus_tag="IWI57_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448934.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            21318..21911
                     /locus_tag="IWI57_000771"
     CDS             21318..21911
                     /locus_tag="IWI57_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448935.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            21933..23957
                     /locus_tag="IWI57_000772"
     CDS             21933..23957
                     /locus_tag="IWI57_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBC2448936.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            23957..25156
                     /locus_tag="IWI57_000773"
     CDS             23957..25156
                     /locus_tag="IWI57_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBC2448937.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            25172..25816
                     /locus_tag="IWI57_000774"
     CDS             25172..25816
                     /locus_tag="IWI57_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448938.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            25827..26633
                     /locus_tag="IWI57_000775"
     CDS             25827..26633
                     /locus_tag="IWI57_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448939.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            26656..26885
                     /locus_tag="IWI57_000776"
                     /pseudo
     CDS             26656..26885
                     /locus_tag="IWI57_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26906..27979
                     /locus_tag="IWI57_000777"
     CDS             26906..27979
                     /locus_tag="IWI57_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBC2448940.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            28023..28292
                     /locus_tag="IWI57_000778"
     CDS             28023..28292
                     /locus_tag="IWI57_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448941.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            28569..28847
                     /locus_tag="IWI57_000779"
     CDS             28569..28847
                     /locus_tag="IWI57_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448942.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            28886..29308
                     /locus_tag="IWI57_000780"
     CDS             28886..29308
                     /locus_tag="IWI57_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448943.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            29328..30920
                     /locus_tag="IWI57_000781"
     CDS             29328..30920
                     /locus_tag="IWI57_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448944.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30950..31285
                     /locus_tag="IWI57_000782"
     CDS             30950..31285
                     /locus_tag="IWI57_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448945.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            31426..>31613
                     /locus_tag="IWI57_000783"
     CDS             31426..>31613
                     /locus_tag="IWI57_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448946.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11367 a         4883 c         6442 g         8921 t
ORIGIN      
        1 tttagaagag gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta
       61 tacgatcaaa aattgactga taatccaatt ggattcggct tgctacctga agatgatgaa
      121 ggtaatgaat ggtttaaaat gacgcttacg gatgataatg gagacgaatt atctgttgaa
      181 gatacttggt catatttgag tgattatatt gtcagcgttg aaatcattga atttgtagcg
      241 gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca
      301 cgacgaataa taaaaaggta gtattacctt aggcattgag taaggaattt atttaggagg
      361 aaaattagat ggcgcgacca gttttagagg cgatggatat ggatactttg acaaaaggaa
      421 ggtatgtaca gctgttagct cgtggttatt cacctaaaga tgtattcaaa tccctgtcta
      481 aaggaacaga tatggaaaaa gaaagattac gaaaagaatt cgattattgg agaacccata
      541 atggaatcaa ggatttgaaa ccagcacggc cagtaattaa acgaaagaaa aagataaccc
      601 aagactgaag tgtagagcgt atgtgatcat aggctttttt atttttgcaa aaaacaagaa
      661 aggtaggaaa actcatgaat tatgaagaaa tcgaaaacag aaaaaaagtg tcgaaagaaa
      721 tggaagaaaa gttgctaaaa atgatgaaac aaaaacacct taaaagatta tctgtcatgc
      781 aatatatcaa tgatatgaag ataacaggaa aagagaaagc ttgtttgtta ggttcaatga
      841 aaaactttga acagttgagg cgaacttatg taaaaacagg ttccaattgc caactattat
      901 tagaagtttc ttgacgatta tgatatgaaa atgatcaatt atgatgtggc attgttattg
      961 aataaaaaga atagtaaaat ttaacatggt ttatcttggt tagatgtagg taaattaatt
     1021 catatataga aggtagatag atgaataaaa acaataaaac tttatttaat ccttacgtgg
     1081 gcgtaatcat tgtttttctt ctttttagtt attttgctta tcaaggttat caaaaggaca
     1141 aaatcgctga aaaagtagat ggtgtaacaa aaacaatcca tattcaggaa caggctaaaa
     1201 cctatcaaga taatttaacc agtttaacgg atatttctaa gagacaactg attactaatc
     1261 aaaaacaggc attagaaaag gatctcaaag gtgaaattgc tggaaaactt gttttttctt
     1321 ctacaggatt tattctccct gtttttcaag gagcgaatag cgacacgctt agtttaggag
     1381 tagcttctac ttattatcta gatactgaaa tgggaaatgg aaattatatt cttgctggtt
     1441 ataacgtaga tacaccaagc gttttattat ccgatatgac tgatttatct gaaggagaag
     1501 aaattgatct aatcgatgct cattacaagt atcagtatga agtctccaaa agaggaaaaa
     1561 tttctgatta cagccaggta gttcaatcta aggaaaacaa ttccttttta tccttgccaa
     1621 aagcaggtaa aaaaccacta ctcacactgt tagcctttca agcaaataat atgaaaaagt
     1681 gcgatgtgat ccagtgtgaa ttagtcaatg tagaaaaaat caataaataa gggaagtgag
     1741 tcacattcaa gaacttgtag aaaaattgat caataaaatg agtgccattt ttctgttact
     1801 gttggtatcc attctatatt taatatggac ggtggggtta aatcctccta cgatattatt
     1861 tatttatgat aatgctgcac agttagtgac gtttttcgta ggctgtttgt ttcttttgaa
     1921 tctgtacaag gtaaagagaa acgatctatt cttcattgca gcaggcattc ttttttacct
     1981 tttcttttct ttttttagaa gctaccgagg agatactttc tatggtgatc tgatcgttcc
     2041 gacaattatt gctattattt tgattgtaaa atggacgaaa tttactgtaa ttgatcgtag
     2101 cttgtatctt gtagtattct atgtcttttt actcgtaacg gtttatcgag tatttactga
     2161 gataccagta ccagaaggac aaagtatttg gaatccatcg aacaaattat cagatatttg
     2221 gatcaataca aatacgatag gtagttcttt aatgacatta gcccttctta tcacaggatt
     2281 tgctagttca tttggaaaat ggtacatgcg attgctatca ttaccagcgt tgattcttgc
     2341 tggggcaacg atttgggttt gccaatcgaa agcagcatta tatgcgcttg ttatcttcat
     2401 tatcttagaa attttaccta agaaggtttt tcgaaataca cacttgtcct tttggatgta
     2461 tgcagcagtt tcggttctag ctttaccgat ttcttatttt gctgcaatgt cagacaaagt
     2521 tcacttattt acaggacgag aagaaatttg gcgcaaattc tatgaaacat taggcgaaaa
     2581 aacaggacaa atatttgtag ggatgaaacc tttctttttc catagaggaa atcagattct
     2641 aggtaatcat aatacgtatc acgcactcgt caatcactat ggattgattg gttttgttct
     2701 atcgattatt cttttgctta gtttcttatg gttgctcgta agaaaacgtg agctgacaaa
     2761 tggacaacta acattcttgt gggctttttt agcgatcatg tgtcaatcgt tcatggaaga
     2821 aacattaacg acaatcagtt gggtgccaat tgtattctta atgttagcaa tggctacaca
     2881 ccaatataaa accaccagat aaatgaaata gaatagtaat aagagaaaac actttttcgt
     2941 tgtgaaaaag tgttttttgt tttgtctgaa aggaggcaga tggatgaaaa aatggttatt
     3001 tggtttttta ggagtcgctt taatcgtcgt ttgcagtgtg tttggctatg ttagctacca
     3061 aaaacatgaa ggagaggtgt ttaagcaaaa tatagaaaaa aagatgcccg tagatcaaat
     3121 caatgcacac gcaaagagtt ataaggagga tgcaacgaac gtcaataatg atatgtcttt
     3181 ggggcaaatg ttgtccatac aaaaagaagc aatcaaaatg ggcgtcaata aacaagtatt
     3241 tgcacaaatt cagattcctg cacttggttt agcattgcca atttttaaag gggcaaatca
     3301 atacactttg agtcttggag cggctactta tttttatgaa gatgccaaaa tgggcaaagg
     3361 aaactatgtc ctagcaggtc ataacatgga aatgccaggt gttttatttt ctgatattca
     3421 aaagttgtca ttaggggagg tgatggactt agttagtaac gatggcgtct atcgttacaa
     3481 ggtaacgcgt aaatttatcg tacctgagta ctttaaatta atcgatggcg ttcctgaaga
     3541 gaactctttc ttgtctttac caaagaaagg agagaaaccc ttattaacgt tgtttacttg
     3601 tgtttatacg tcacaaggaa aagaaagata cgtggtacag ggagagctac aataacaaga
     3661 atttaatcaa cgcgagtcgt tgaaatatga aatgaggaga gtaaaaaatg gcaataaatc
     3721 ggtatgtaaa aaaagctgga atgattgttg gaatgttagg aatcattgca ggctgctttc
     3781 aggggtatcg agcttatgca gaagatgtca cgcaaaaaac gccaccagaa agggtcaata
     3841 tcacggttca taaattaatg tacgatcaaa gtacgcaatt gaacgttgat attgacggaa
     3901 tcaaaaatga tggttatacg cacgatcaat atcctacagg tgtaacgaaa tacaacaagg
     3961 cagattatgg tgatgttgag tttacgttag gaaatattac ggatcaagta ttaccgactg
     4021 aagattctga tctaaccaac gcgaaagtgg acgaaatcgt taaagatgta gaagacaagg
     4081 gatctaacag tgagtacgtt aagaatgcaa ccaataaaat tacttcagct gtagatgaaa
     4141 atggtgagat cacttttgct gatcaaccag cctatgtcaa ttccaaaggg aatgtatatg
     4201 tggtttatga aagtaaatcc gctgctggat tagtcacaca aaaagcaaaa ccaatggttg
     4261 tgattgcgcc aatgacagac aatacaggat caggattttt aaaagatatt catttgtatc
     4321 caaagaacat tgtttctaag ttgagttttg aattaacgaa atttggtgac gatggcacag
     4381 ctcaaagcaa acaaacacct ttaaaagggg caaaatttga gttatacaag ggcgaacctg
     4441 gaaaaggaac aaaattagga gagcttgtat cggatgatca aggaaagctg actgcaactg
     4501 atttgacgtt aggaaaatac tactttgtgg aagttccttc agaagttgta gtgggatcag
     4561 atcaagaacc aacagcagac caatatttgt taggcgcaga cgctcgcaac gatgcgcaca
     4621 ataagttaac ctttgaaatt acgaatgacg gcgtgacaag tgatttaaaa gcttcctacg
     4681 tgaactataa agcaccagtg atcgataaaa cagtgacaaa tggaacagga gaagaacatt
     4741 ctttccaaat tggtgacgca gtgaattatc aaggtacaat tcatatccct acagatatcg
     4801 caggtggaac agatgggatt acagtaaatg gtgtaaaaag tgaaacttct ccttacagtg
     4861 tatttaaatg gggcgacaca gcaggtaaag gactttctta tgttgctgaa aaagcagata
     4921 ttaaagtaac aaataatgat ggttcagtcg tattaaaaga aaatactgac tataaaatcg
     4981 aaaattctga tagtggtttt gtgatcgatt ttatcgtaaa taatggacaa gtaagtgata
     5041 cagttgcgaa tttacatggt caagatctaa aaatgactta taacatgttt gtgaatgata
     5101 gtgctgaaat cgcaaatcca ttaacaaata gcgtggattt cgtttataac aataatccat
     5161 ttaatcagga agaacaccac gaaaaaacga aagctgacgt tgtgacttat ggcgcaaaat
     5221 tcttaaaagt cgatagcggc ctatttggaa caggtatcaa agcaacaccg cttgaaggcg
     5281 cagaatttgc agctaagaat gcagaaggta agtactacgg tggacttact gatacagata
     5341 aagatggtgt caaagaagca gtttgggtag acgatgtagc aaatgcagca atcctaaaat
     5401 cagataaaga gggtcatttt gaaatcactg gtttaactga aggagattac tcactggaag
     5461 aaacaaaagc tcctgaaaac tatcaaaaat taacaaaaga gatctctttc aaagtagaca
     5521 aagattcata caaagaagaa aaccgaatca cgatcaaaaa taatcaaaaa gcttccgtac
     5581 caatgactgg tagcaacggt ttccaatctt acgtattgat ctcttgcttg ttgcttgggg
     5641 caggcgcaat ttctgcagtt gtttacttta agaagaaagc ctagaaggat cagctaatga
     5701 ttatcagaca ccctaagaaa aagcgaatca tgggtaaatg gatcattgct ttctggttac
     5761 tttcagctgt tggtgtctta cttttgatgc ctgctgaagc gagcgtggcg aaataccaac
     5821 aaaatcagca gattgccgca atcgatcgaa caggaacggc agcagaaacg gattcttctt
     5881 tggatgttgc taagattgag ttaggcgatc ctgttggtat tcttacaatt ccaagtattt
     5941 ctttaaaact accaatttat gacggaactt ccgacaaaat tttagagaat ggtgtaggga
     6001 ttacggaagg aactggcgat attacaggag gaaacggtaa aaatccttta atcgcagggc
     6061 acagtggact ttataaagat aatcttttcg atgatttgcc ttcagttaaa aagggagaaa
     6121 agttttatat caaagttgat ggtgagcagc acgcttatca aatcgatcgg attgaggaag
     6181 tgcaaaaaga cgaactacaa agaaactttg tcacttactt agaaccaaat cccaatgaag
     6241 atcgagtaac gctaatgaca tgtacgccta aggggatcaa cacacaccgt ttcctagtgt
     6301 atgggaagcg agtaaccttt acaaaatcag aattaaaaga tgaagaaaat aagaaacaaa
     6361 aattatcttg gaaatggctt ttaggaagta ctgtttttct ttcagtcatg atcattggtt
     6421 cgctatttgt ttataaaaag aaaaaataaa ggagggtaac caacatgaga aatagaaaat
     6481 ggctatttgt ttgtgcagcc gctctttttt ctttcactgc ctacacacag ttgacgtttg
     6541 cagacagaca agaaagcacg gatagcacag aacaaacaat ccaaacgtca gatagcagca
     6601 cgattcaaag cagcgatgaa accgtcgatt cgtcaacagt tgattcttca gaagaaccta
     6661 aaattgctga agacgatcaa atagtcaaac agaccattca cgttcaaaaa attatttctt
     6721 ccgaacaatt agacaaccag gggcaaaagc ttgaaaaaca agaaggagta aatggcgcga
     6781 agttttctgt ttatgatgta tcggacattt tacaaaagat ggatgttaag gatctcacta
     6841 ctgaccaaat tgaatcccaa ttaaaagatc gagtgaaaaa actatccagt gatcaactga
     6901 agctaatttc taatggagaa acaaagacaa ttgatcaaca aacaggtgtc tttgaattct
     6961 cggtagaagt acaggcaaat cagaaacaag cttattatat tgtcaatgaa tcaagtcctg
     7021 aaaacatttc taatagcgag gatattttgc tattaacacc tgtttctgat aaaaatggtg
     7081 aattcttaaa ggatgtgtgg atttatccta aatcagaagc ttcgcaacct aaagaagaag
     7141 taaaaaaaat cgtatctact ggcgtaaaga aaaacttctt tgagaattgt tgggattttg
     7201 ttactcactt gttttctaga gattaaggaa gaaataaaat gaaactgatc aaattattat
     7261 tattcaattt ttcttgttta accgcagtct ttttgtactg cggtttatta ttttctaaac
     7321 tatctatttt gccaagctac caactatacc taatgaggat tttaaacata ccagctgctt
     7381 ccatgccgct agatattatg gaaaagctca ttaggaatcc attatttact acgatcaatt
     7441 tgatcgttat tgtcggaatt tcactagtca tttataaaaa agaggggatg aagggatgaa
     7501 ttttaaacac tcgtggacaa gacggttaaa atatgttttt cccataatga tgatgttagg
     7561 tacattgttt gggttaaaaa cagccaatgt ctttgctgaa aaagttattg tcgatccgtc
     7621 aaatccaatc cagaacgtaa agaataattt tattattcca aaagatgttc ctaatgcaaa
     7681 aacaaatatt actgtaaacg gagcgtcaaa agtttacaaa tatcatgttg atgccaacaa
     7741 aggtggtttt acagacgact acgtaggatt agtggaggga aacaaaaata tacgctatcc
     7801 ttctttccaa aaagaatacg gtgaaacaaa ccttgtttta gaaggcgtaa caaccaacac
     7861 caaagtcaat attgactatg gaaaaatcgg tacgtataac gggaaaaaag tcaatatcaa
     7921 gctagttcta tctaatattc acttatactc ggatacgttg ccgtggaatc tactagataa
     7981 caactataca aaaactcatt ttcgagacga tggctataaa aatacgaatg gtgcaatgag
     8041 taaatctaaa aaaagaacag tactttggat ctcagacaat cttttctcag gaattgttta
     8101 ccattcaaca caaatgaatg tgcagctagt agccacatat gaggacggta gccccgttca
     8161 attttcagga gatacattta tcagttttaa ctctttgaac cctgcaggag gaaaaagcac
     8221 ggatcttaaa ggagaatatg cccactatga caaaatgaat acgacagatt ggtatgtcag
     8281 aaaagatacg gttctttcag aatttaaatc tttctacaac aatctgaacg tggtaggtgg
     8341 ccacccagga ggaagctcta agctgacaca agcggataat gatttcaata atttgcatga
     8401 taaattagga gatccgaagt ttggtcaagg tactgtgtcc tttaaaatat ctgaagcaaa
     8461 tccgacgttt gtgattggtt cttctaatgt tcaaacctgg tttactttat cttctgcgac
     8521 aatctttagt gttgttcctg atcaacctga aaaaacaggg gttgataaaa atggaaataa
     8581 cgtgaacgat aaaatgttgc aagtaggaga cacgattcaa tatcgaatca aacagaaggt
     8641 gaatcgatta ggagtggatt tactagcggt ttacgatcga tttgagttaa tcgataattt
     8701 accaaaagaa gtaaattacg tcgatgcaca cgttgaaagt ggtacaaata aaaaattcga
     8761 tgtatctggt gaagtgacct atgacaaaac aaaacaccag gtaaaatatg cagcgaaagc
     8821 tgatacatta aaaaatcgaa tgaagtataa cggcgaaact tacgaattag tgatcaatgt
     8881 aaaagtcaat gaattggcca atcaaaatag tgtagccaaa aatcagggaa cgtcgatcat
     8941 taataaagta gaaaaggata caaatatcat taccgtttat ttccctaaaa ttccagtgaa
     9001 agaagtacaa caaaatggaa aagatgtaaa cggacgaaat gacggtaaga agggtacgcc
     9061 gacagctcct ttaaacgcag gatctgaagt acaatatcta gtcacacaaa aatggcacac
     9121 aaaaggcgta gacgctgcgt cagatcacta caaacaattt agtattcaag atccaattga
     9181 agctcgtttg acatataaag aaggttctgc gcaagtaata gataagagta aaggaaaaga
     9241 tattacttct gaaggcacac taacctatga tagcaattca agaacgctga agtgggaagc
     9301 ttccgctgac ttcttaaata acaatctttt agatggacga gaaattcagc tgatatttac
     9361 agctaaaact ccattacaat cagaaaaaaa tattgataac caagccgtag tagcagtaga
     9421 taatgtttct aataaaacaa atgttgtgac aattggggtt gatcctaact tgccacaagt
     9481 tattgttcct aaaacaggtt ctacacattt agtaacgatt ttagcggtca gcttactatt
     9541 acttgtgtta gcaacttttg gctacgcagt tctgaagttc aattaaacaa aaaaacaagg
     9601 ctgaaaatca gccttgtttt tctatttgaa aatgggattg gcactaataa atacgttgtc
     9661 tgaagaaagt gatccgtctg cagaaacttc ataagcatgg gataaaccaa cgatactttg
     9721 attgttgtaa agcaagtttg cgttatgagc agcgctatct ttccacataa ccatagcatt
     9781 tactgctcct gtatctgaaa ggatctcgtc aacgtaaagg tagaaactat tgatattttc
     9841 tcctttagca ccgtagcagc ctaagctttc taagagatct tggaaagtac cgccgccagg
     9901 tcggtaatga ctgaagctta cttgtaattc ttttgcacga atatctgcag cttgttgaac
     9961 gatatcattg aattgcattg gggcaacgcc attttgttga cggtagctat ttaaccaagc
    10021 aagttctttt tgacgcacat agttgtttaa atcacttagt gagatatttt tgtatttgaa
    10081 agtgatcgtt cgattttggt caacattatt gattacacgt tgtccatttt tatcaacggt
    10141 aacagatgaa tcgtcatttc tacacaagaa gtagttttga tcggtcatat ttttccaaga
    10201 agtagagaaa ctatctccct cgtaaaccgt ttgtgtttca gtagataaaa cagcgtttgt
    10261 gtcaacgttt atatggttca cagtgacgtt gtattgatga gtataggcaa aattgattcc
    10321 ttgattactt gtaacgttgt ttaaagtcac agtgttgcta gagctttctc taggatcaag
    10381 aacaaaatta gtgaacatag gtgcttcaat tgtgatgctt tgtccataaa cgacgggata
    10441 actcttactg taattataga gttcttggat atttggatca gtatccgaga ctttatttgg
    10501 acgagctttc acttcaacta gaaagacttc atcatagtaa aaagtaaatg tggtatttgt
    10561 gttaatcgtt tgtgtttgtg tttcattccc atttacttga tactgtggtg cttcaaagtt
    10621 agtcaagtcg gttctagctt ttgcagtgta acttgtacca gcttttactg agacatgttc
    10681 ttcttttagc agcgttccag tttgtcgatt catatgacgg acagttaccg catattctga
    10741 aggtttaggt ttgtgttgta accctttaaa tgcagaatca agactatttt tagcattatt
    10801 tacttgggtt tgagaagctt tagaatcgtc tagtactttt ttagcattgg ataaagcagt
    10861 ctgaaaatta ttccaagatc cttctgtaaa atctccttta gcagttgatt ttacttggtt
    10921 gtacaaggct tgtaattccg ttttgttcac agtaggagct ggttttttct ttaatccctt
    10981 gtaagcagaa tctaatgtgt ttttggcact atccacttgt ttttgagtcg cttttgagtc
    11041 atttaacact tttttagcat tgtttaaagc ggtttggaat gcgttccagg tattgtcggt
    11101 atagtcacct tttttagttc ccttaacgct cgtataaaga gcttctaaag ctgatttatt
    11161 cactttgggt ttagaggtgt cagcaccata ccaaccaata ccttcgtatt tccagccata
    11221 gtctttggtt aaccattgta cttctttcca acttgcagtg tagtgatgcg aaccagaggt
    11281 aacacctgga aggaaaaggc ggtagatcgg tttattattt ttttcggcag aacgccaggc
    11341 aggcccttca taagtccaac cgtaattttt ggtcaaccat ttcacttcat cccaactagc
    11401 tgtgtaatgg tgatcacgaa ggcctttatt gtataaacga tagacaaggt ggccaccgct
    11461 agtcggtgct tcccaagcgg ggccttcata attaccccac ttaattttca ctaggtgatc
    11521 acgttctttt gtgctagagg tgtaaaaatg ttcatggtta tttggattat aaaggcgata
    11581 catgacgtta gttgaagcag cgtcagcaga tccagcaaga gcaactgtgg cagcaaatgt
    11641 tccaatagct ataacagttt ttgggtttaa taatttcatg ttttcaactc cctattaaat
    11701 gattaaatat tttgtttaca ttcataatat accacggtat accgaggtag tcaacggtat
    11761 accgtggaaa ttttgaaata aaaaagccgc cctatgaaga gtgtaaggat ggctgtttat
    11821 atgctatgaa gaaatggatt cggcgtattt ggttagtttg gcagcattat caaatcgcat
    11881 tttttcaatc gcactttttt tctttattaa atccgcaatt gttgattctg cgactgaaga
    11941 atgtttgcta atttcatatg ctgtcttgcc agtttcaaat agtaaccatt cgattttctc
    12001 tttatcagcg tacattgcaa tgctccctct tgattttttc tttattatag ctcttttttg
    12061 taataaatca caatatcaag aggaaaagtt tctatagaaa ggtgggaaat tatgagtatc
    12121 cgttccccgt gtatcaaaat gaggtttgtt gctactttcg ttatttaata tactaaaata
    12181 aaacaacgaa agaaggaata gaatgaaaaa gttaataatg gtatttactt cgttgattgc
    12241 catgattgtt attttaggtg gatgtggtag tgaaaaaaat aagcaagata gtcaagataa
    12301 tggcgtgtta aaaccaggaa cagtgtggaa agaagaagta ggcggacttg ttcataattt
    12361 aaaaattata gatgatacaa catgggaata ttccgaaagt gtttggcatc ctgaacaagt
    12421 acaaataaca gttgagaagc taaaagacta taaaggatta gaaagatata aggtaatcga
    12481 ttcaggtgga gtagatttat tcataggtaa aacagcctta atagttccct ataacaaaga
    12541 cggtttagaa acgtttaaat ttattccaac aaacgaaaaa gaacatcgag ataaaagtga
    12601 aatattaaaa gaatacagta ctaaatctgg atacaagtta caaaagacta gcgagtgatt
    12661 gctagtcttt ttatttatcc tgaaggagga atcgcagtgg caaaagctaa aacgccattt
    12721 gaaaaagccg cgtctaaaat cttagaacgt cgcggtgtat ctgaaacaca gtggaaacaa
    12781 gaagtccttg aaaaagcaca atttgaccta ttacggggaa aagacaagga cttagaagga
    12841 tatatctatc aacgtgaacg tgaaaagctt gttttcgatg aaattattaa acactaaaag
    12901 gagcgaacaa ttttatgttt gaatcaatta caatgaattt tttaacacaa ccgaaagtag
    12961 tagctgcggt tgatattttt agagcattac aaggtgcagc aactagttta aaaaacaatc
    13021 tgatgatttt tgcggctgta gctgccgttg cctccattgt tcttgggggg attttcttct
    13081 tctttggccg aagaggagct gaaaacggga agatgcacgt aggttatgta ttgatggggg
    13141 taggagttat attagctgcc gcctctattg tggtttcgtt atttggaatg tttggacaaa
    13201 ccccacaggg actataaaaa ataaatagaa ggagtaaaaa gtacgtggct actttttact
    13261 ccttttttat gaaaggagaa tactcatgta ctttacgatt tcaggcaata ccaaaaaaga
    13321 taaagagtta ataaaaaaag caacgaagaa agtgggaaag cgtactgcct ttttaccgaa
    13381 aacaattaaa tattctgtca ttgaaacgga agaacagcta catgtgttag gacaagtgtt
    13441 agaacaagag aatcaatctt ctgttaaatt gactctcacg ttacatgata aagaaggaaa
    13501 aatagaaaag gctggcacag aatacgtttt tggcccactg gattatcaaa tttcagaagg
    13561 tttttatcct acattgatgg atttgatccg ccttgatgtg tttaaggaaa cggatgaaac
    13621 catgagtgtg gaagagtctt atcgaaacga aaaactgcta caagttttag aagaagagtt
    13681 agagagcgac caaagaaaag tgagtcaagt gagtgaagtc gaagtaaatg atgaagtacc
    13741 atgggaacga ccaatctttc caaagaagga gtcaaaagaa aaagagacag tctatgttga
    13801 cgaacaagct gatattccga ctcagaaatc gatatttgag gaagagaacg atgtaatggg
    13861 tcaattggag gtagatccct ttgaagaaac aatggaggat gaagagacgg ccgaagtaga
    13921 agttgatcta gttgaagcta atgaagagcc aacatggact gaaggggatt ctcaggaata
    13981 tattgaacca cttatcagtg aagaagtaga gaaaaacgaa acagagatac aaaaggcaac
    14041 tgtatttgat tatattttgg atcaatatcc gccagagcat gaatggatga aagaaaaaaa
    14101 taaattattt ttaaatgagc tttattcgaa atatcaatta ccccaaacct atcaagcatt
    14161 tttagagagt aaagaagaat taatctcaca gggtaaaggc acacttttag aacagttaga
    14221 gtctgttcaa tcaacaaatt ggcatgagct tgcacaagaa gatttattag ccacttttga
    14281 agaacgtcaa caagaaacag atcaagaaat aaatgagtat gcgacggaac agtcatctaa
    14341 ttgggagaga acaaaagagg aactaaataa agaagaacaa cgagtgattg aagaagaagt
    14401 acaaaaaatt aaagagcgat acgaaaagaa acgcgacaat gcgtattcct tgtgtataga
    14461 gaaaattaat aactttacta aaactaatca agaagcttta tcgaaagaaa aagaaaactt
    14521 gttaaaagaa cgtataaaag aacgaaaaga agagttctac cgaggtttac gtacagataa
    14581 gctaaagatt agcaaagaat taaatgaaca actgaataaa ctctatcaaa ctacgactga
    14641 agttcttatg gaaagacacg ggcagatcca gcaagaatta caagcacaaa ttccaaaatg
    14701 gaaaaaagag tatgaaattc aacaacaaga attgaaagaa caagaagaaa aacaacgaca
    14761 agaagaaaaa gagcgcgaac aattagaact aaaaaaacgt gagcagcatc ttgcagaaca
    14821 aaaagtggcg atagaaaaac aacggttaga gcaagaaaag gaaaaagaag cagctgctcg
    14881 atacattgcg acaagcacag ttgaattatc tagcacaacc tcaaccagta gctgctgttc
    14941 aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc acagcgttcc tttaacggat
    15001 ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt
    15061 ttaatcatat caatcaatct tcacaggcag attctgtagc cacaacggcg tcaatcaagg
    15121 aagaagccca ggaacaagca agaaaagagt atgaacaaca attggaagaa ttattgaatc
    15181 gtaccaatca gtcaacagca gagtcaagta tggaaaatag caaagagagt gatcctagta
    15241 aggctaaaga aggatcaagc gattcaactg tacactcaaa cacttccgaa agttcagaat
    15301 caaatgaggt gaaataatgc gtattagaga aacaacgaga cgaaaagaca aacgattcaa
    15361 tttagaaaag aataggtatc tttctcattt ctatcgctat actccttcag gagaagagct
    15421 accagaaaaa catgacaaac gtatttctca aaagattttt tatagtggct tagcttatct
    15481 tcctttaaac tatgttgtta caagaatacc agaagcattt catcgatttg agattattcc
    15541 aggaagttta caaggagtga atatttttca gttacaagaa atgccgcagt tattccattt
    15601 tgattcaact ggtttttgga atcctttgac cccaacaaca agtttaggca cacgtttagt
    15661 tgtttctacg atttgtgttg ttccagctgt acttatggga ttgaaaatgg acatgtggtt
    15721 tcgtccgcta gcagatgggc aaaaaggaga taaccgatta aaaacgttaa aggaaatcca
    15781 acaaatttat ccgcaaatac cagaatcaaa gggaagtttt cctggttatg gcggaatacc
    15841 aattactcat tataaaaaat actggtatat tcagacggat acagtaaata cttgtattgt
    15901 tggtacttcc cgctcaggga aaggccagat agaagtttta gcaaccattg acaatttgtc
    15961 gcgtgcagaa agacaaagtt caatggttgt taatgatcca aaaacagaat tatatgttgc
    16021 tagtaaagat gaactagaag ctagagggta tgatgtgtat gcctttaata tcaccgatcc
    16081 actgcaatca atgagtgata atccattggc actcattgtg aaatactgga agcgaggaga
    16141 catagacacc gcaacacagt taaccaatac ttttacgaat acgatttatt atgatgcaaa
    16201 tgcttcagac aataaatact ttaacgaaaa tgcgcagaaa gctgtcaatg cgcttatttt
    16261 tgcgttattg gaacaagcgg atcaaacagg ggattactcc aaattaacac caaataatct
    16321 cgttgaacta ctttcagaaa ttggcggttt taattattat ccagatcctg agaatgaatt
    16381 caagcagctc aatgccttag atgagttcat gaatcagtta ccccctggaa atatagcaaa
    16441 aaaacaatat ggggcaacaa aaattggatc tgataaagcg aaagggaata tcttgtctac
    16501 ggcaattaca ggattgaatc catttaccct aacgaaaatt gcgaagatga cttctcaatc
    16561 gaccattgat tacaagtcca ttggttttcc gaagtatttg gatctcaaat tacatgaaag
    16621 cctattaaat caacgcattg tcgtggaatt ttatcataag ggaaagaaat ttcacgagga
    16681 acgagtcaaa gttggctatc gtggtttttg tgaaatcaac tttgattgtc atttagagca
    16741 gggagatcat gtttgcttaa aatacaatca tcaaggaaaa caatatgttg cttggtatga
    16801 gttttctcaa aaagtattga aagacgaaga gggaaaaatc gtttatcgaa aaaaacgagg
    16861 agaaactcat ttgccagaac tagaaaaaga agccgttcta acattagatg agaagaaaag
    16921 taacctatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat
    16981 gttaacgcct gattatgatc catctaacca tatcattgtt agcatctttc ttagtcagtt
    17041 gtataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgtaggat
    17101 tcattttatt ttagatgaat ttggaaatat gccaccgatg gacaacatgg aaggaatcat
    17161 gacggttacg gcaggtcgta atatgttatg ggatctgttt attcaatctt atcagcaact
    17221 ttatgcgaag tatggagaac aaaacgcagc gattatcaaa gaaaattgtc agacacatat
    17281 ttatattatg tcgacagatg ataataccat cgaagaattt tctaaaaaag tggggaataa
    17341 gacggttgaa caagagaaca gtaccatcaa ttcattaggt atgaatactc atatcaatcg
    17401 caatgttgat tcagatcgta tcttaactcc tgaaagaatt tctacttttt tagaaggtga
    17461 aacgattgta ctcgctcctt taaaacgaca agatttgaaa ggtcataaaa tacggccgta
    17521 tcctattttt aatcatgggg aaacgaatct tccttatgct tatcaatata ttgaagattt
    17581 caatccatcg tgggatatca atgatataga catacattgt acacatgcgg acttggatct
    17641 taaggcttta caaattgatt atgattattt cctaccagat gaatatgcta gaaatctcta
    17701 tcatggactg catgatcaat tagaagaaaa aaacgaagaa aatatgtttt ataaaatcgt
    17761 tcaatctttt gagaatgaga cggctaaaaa attgttaatt caattttacg aagaacaagc
    17821 aacggatcgt cttcaagaat atgttgaaaa gttgatcaaa aatgaagaaa tttctgaaat
    17881 gattaggaaa cggctgttat tggaagctga aaaggcggtg aaagaatgat cattggagga
    17941 attttctctg gcatagccga ctggtggtca ggtttattcg gagaagggga tgcagatccc
    18001 aaaaccgtaa cgaaagtctt acaagattat ggtaattatc tgcagtatca agatgtattc
    18061 agttggattt gtaatacttt tttatgggga ttgatcaaag ggttatataa tctcaatact
    18121 ttattagaaa aaacaattta tcaaagcttt gatttaaaag acttattgaa cgccgctggt
    18181 gttaatgaac tatttcaaag tttgattagt aaggtattgg ctcttttttg tgtcgtcaca
    18241 ttaatttatt taggcttgaa attttttatg agtaaacacc caccgaaagt caaaaatatc
    18301 ttggttaatt tatttatggc catgatcttg atttttggtg gtagttcttt aatcgatgaa
    18361 gggttaaata tcagtaaaaa cttttatgga gacgtcactg cagcaaataa agcagataaa
    18421 acgggttcac ctgcttttca aattattcaa aataatgtga ttgatgtcag tacattacta
    18481 gcgactgacc cgaataaagt agaatcctta ccaacagata aaagaaatgc tttaacagcg
    18541 aaaaatttca aaacagcgaa tatcaatggg attattacac ctgatcaagc taagaaaatg
    18601 tctgaatcaa gtgatattag tacagtacaa aaagatcggt taaaggcatt acagtatcga
    18661 ctagagttag acgataatgg gaaagaaatc cctgttcaaa ttagtgatga aggtttagca
    18721 aaatatgtgt atacgtcagg ctatcgtcga tacattagta gtccagggat tattttggct
    18781 ggggaatctt ctttgtcggt agcctattta tttattttgt ttacaatcgt tacttgcatt
    18841 attgagctag tatttaaaaa attctattta gtaattgccg cgtcaacgga ctttgagaca
    18901 ggacaacgga tgaaaagtgc gatagaagat atttctcaat ccttcttgtt attggcgttt
    18961 acgattttag aattacgaat ctatacatta atgctttcag gtatcggtga tttgcatgcg
    19021 gtaggaaaga tcaatggatt cttgtatgtt gttgctttaa ttgtgctaac tattgcgcta
    19081 ttcaaaggct ctcaatcggt tacgaagatt ttcggtgttg atacgagtct gaaaaacggc
    19141 agcaattctc ttcttagcat gtttgcctta ggaaatattg cgaaaaatgt gggtagcgga
    19201 gctaaaaacc ttgcaagtgc tggtaagagt ggtttaagcc atttaaatga tattcgtaag
    19261 aatggatttg gccatcgaaa agatattacc catgataacg gggaagaaca atcagacgat
    19321 aaagctacaa ctgtacctaa tagtaagaag acagtttccg aaatgttcgg gaaagcgaaa
    19381 aatggtgttt ctaaagccgc agaaggtctt ggttacgtca atgaacgtgg gttaaaagga
    19441 acagttgaag atcttacaga aaaagccaaa gacaccttag aagagaaatt agacggcacg
    19501 agagctaaag aatttgctga agctgtgaag aatccatccg aagcactaga aaaacaaaaa
    19561 gaaaaagctt cagcagtcaa agaaggacta aaagatgcgt atggtgaagg acaagtcaat
    19621 gcagcagtga aggcaaatac aacaagtgaa aaagaaaatc aagaaggtgc agcgattcct
    19681 tcagccaaag atcaccaaga tgagttacat tctaacgaaa attcgatcgg agaaaaagat
    19741 tcagaagaag gacaacatgg tcagttacca aaaaccgatc cagaacaata tcgttataaa
    19801 tctaacatta attctgaaaa cgaaaaacgc atttcagatt taccaaaacg aaatcaagaa
    19861 gtacaaacgg atgaggattc catcgatctg ccaaatgata atggcatcga agttggaact
    19921 gaaaaatcaa cgaaaattgg tttgcctaag acacaaaatt taccaatgaa agatagtcgt
    19981 ttgaatcaat tcaaagagaa tgatcaaagc tttgttcatc aatcacctgc aggtactgtt
    20041 caaatgaaat caagtcgttt gcctgaaaat ggaaatctga caactgtttc ttacaattta
    20101 gctgaaagtg gtgaaacaga gaatgcgtcc gttcaaaata ttgtgacaaa agaagctgca
    20161 cctaagccaa tcaataaagt tcataatgga tacagaccag caaacgtagc atttactgca
    20221 ccgacaaaaa taaatgtgac aagagaaact aaaaacagga acaatgaatt gaaagaacaa
    20281 ctattgaagg atatcaaacc ataaaaggcc tatttaaggg cctttttctt tataaaacaa
    20341 gtttaaaatg gaaaggagaa aattgtggag gatattttaa tagctacgtt tataactgca
    20401 ctgggagcat ttttaacgat atttgtggtg actattaaag gatttaaaga tacggatagt
    20461 ttacaaaaag atcatcagtc tattttagat ggtcaaaaaa cacataccga aagcattatt
    20521 gtacaccttt cagaaggatt cacttatttg aaagatgcta tgcccacatt cacagtaaaa
    20581 ttaacggaaa tatttcattc tgttgagcaa atatcgtcag aattgaaaga gcaaagacaa
    20641 caaaaagatc gtcaacttgg ctcacttaat tttaggcaag aaacgataca agaatcattg
    20701 gagaatgtaa atcgattaaa cgatgaaatg caacgagttc atttggaaaa tgaagaatta
    20761 cgcgcacaaa attatgattt aattcaaaag aaaagaaatc tggaacaaaa aattgttgaa
    20821 ttaaaagaag aaaatcgtca attgaattat caattacgac gaggaaaaaa tcgttctcgt
    20881 gatttaggaa atgaattaga gttgtaatat ttagaaaaac gctgtgagaa atcacagtgt
    20941 tttttatttc taaaaataca aaatataagg ggtatggtta tattatggat aaatacattc
    21001 atatgaaaaa tcctccaaga tttagtgggg aatggaagtt tttcgccttt ttctttttag
    21061 atctattaac gcttggtggc tgcttttttt gggcgaaaat agtcaataat cttgtccact
    21121 taccactctt ttttgcactc ttgaattatg gattatggct tgcgatagcg ttagtgattg
    21181 tttggcgacc ctataataac cctggtcaac gccaaatttt tgcgattatc gaatctattt
    21241 tctttacgga taagaaccac tatcatgcgt tagatcctaa ccgaaattat catggtacgt
    21301 ataaggagga tgaataaatg cgagtcaaag gacaaaattc agttgataag aaatccattt
    21361 tgcagtttgg aaaaccagaa gagcgctcta tcttggactt atttccatat aaatcctgta
    21421 cagaagaggg agtcttaatt actagagaag atcattttca gcgttattat cgtgtcgttt
    21481 ccactgacgt ggagggatta aatgaacaag agaaactaga acgaatgaat cagttgacca
    21541 ccgtcatgcg tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc
    21601 taacggaaca aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcac
    21661 taggaagaaa tctacagcga ttaagaaaat atgaaaagaa attagtagag gagatccagg
    21721 aattaaaacg ggcagaacaa gaacgtcctg acttaagctt ctttttcgtt attgaaggaa
    21781 agacaatcaa ggatttacaa actagaaatc gtcagttaat gcgatctagt ggcattttag
    21841 ggttaaaacc actctacaaa aaagaattga ttaagattct ttatcgaatg aacaacatga
    21901 atgacgaata aaaaagatgg aggaaatgat tcatgctaga tgtgataaaa acagcttttt
    21961 caacgcaaag taatcaaaag gtgattgatc cattaaaaca acttggtaat cgtgaaatag
    22021 agattttaaa atcaaaaggt tatgatttag atttcttaaa acaagtgatg ccacaaggtg
    22081 ggttgaattt tgatgaagaa tatgtgacct atggaaatgg ttattcacga tgtgtgacag
    22141 tttatcatta cacaaaaaat cctactcttt tttggttggc caatctcatg aacaatcctc
    22201 atacagtggc cacactagat gttagcacag atgaaaagca aaaagtgatt gatcaaatca
    22261 accgcgcatt agatgaatta aatgatcgat cagataatga acgaaaagcg actgaccgaa
    22321 ataaaagtgc ggaagaatac ctggaactag cggaatatgc ccgaaaattg acttctaatg
    22381 gggaaatttc taaacaagtc aaagtgagag tacatgttta cggaaatacg atccaagaag
    22441 tggatgaacg ggcgcaggaa ctgatcgaaa atctaaaagg gatggattat cgtgcggtga
    22501 cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc gatgaacaag
    22561 ctgaatggtt tggaaatatc aaagggcaat cacttccatc gttaaacatt ggcggaggat
    22621 tcccgttcca ccaccaaacg ctaaaagatc caggaggcac acaatatggg gaaaccatga
    22681 caggcggccc atttgtctgg aatgccacgt ataaaaatag tgtacgttta tctaccaata
    22741 ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg atcgcggaag
    22801 cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac tttattcctt
    22861 tcttaaagga atataacggt attatggtgc gtttagacgg atctgacggg atgatcaatc
    22921 ctttagagat ctttgccaca cggacgtatg atgaagcatc cagtttatat gacgatggtt
    22981 cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacgcactta gacaaagtag
    23041 tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca gaattcaaga
    23101 cctacttgaa tcgtttttat gaagagtatg gaacagtacc acgcggtttt acttcctcaa
    23161 attcgcgttc aaatactgat acgcaagtca cagggaaaga tccagaagct tatccgacgt
    23221 tcaaagaatt tttagccttt ttaggacaac tggaactacc aggggcttcc caagagaaaa
    23281 agaatcggaa agaagaaatg gagtctatcg tggaatcctt atgtgagact tatggcatga
    23341 tttttgatgg tcatagtacg attcgacacc ttgaccaaca acagttggtc ttttttgaca
    23401 ttgactctat tacaggccta gatcgagggg tacaacaatg tcagatgtac atggccatga
    23461 cgttgatttg gaatcaggca ttgattcaag gacgaagaca acgacgctta attaaagaaa
    23521 aacaaatgga agaacaagac ttcaaacgct tcttggcgat gattgatgag tgccataatg
    23581 cgattaatcc agatttcatt gaaacggtaa attatgtcac aaacttccaa cgagaaatgc
    23641 gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa
    23701 atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga
    23761 tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtt ggtaaatcaa
    23821 tgacgaactc agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaatt
    23881 ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt
    23941 ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaaacagt atgttaagcg
    24001 tcgtgcaaag cggcgcttat ttttgtgggt gtttggtact tctgcaggtt tgattactat
    24061 attgatcact gtgtttgtga ccctttttct aatacttgcg gcaggttcta ttgataattc
    24121 tgattcaagt agtggtggcg aagcttttac aggagaatat tcggaaggct tacccattta
    24181 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc
    24241 agcagtgaag tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc
    24301 gtttggtacg tcggcgtccg caagaaatga tctcaattat tttgggatta cgtggtttga
    24361 tgggtgtctg tttcctaaag gaactgcgcg tggaattggt ggcattgaag gtggctggta
    24421 catgaagttt cccaactcaa aagctgcctt ctcctattac ggctttatgg tagccacaca
    24481 atcaaatttt aatgcttgtg tcggaaataa aagccctggc gcaagcttat tgatcttagg
    24541 acgaggaggg tatgccgccg caggaattac tgaagacagt gcctattata aaaattgtat
    24601 gtctattatt aatacaaata aattgatgga gtacgatgag tttgcgatta aacactggag
    24661 agaaggcggc ggaactggtg gtacgattac aggaagctgg ggcaatccat tcccaggaag
    24721 tagcttagat aaaaattcgt tcagtggagg acaacttttt gggaaaaatc caggaggaga
    24781 attccgacct aacgggttcc atgatggact agactttggt tcagtcgatc atccaggaag
    24841 tgagattcat gcggttcatg gcggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    24901 tttgggtgcg tgtgtgatcg tgattaatta cgatggcttg aatatggtgt atcaagaatt
    24961 tgcaaatagt acaggaaatt ctcgagtgaa agtaggcgat caagtgaaag ttggtcaagt
    25021 aattgggata cgagatacag cacacttgca tttaggattt actcgaatgg attggcgtca
    25081 agcacaagga catgcattca ttgatgatgg tacatggatc gatccgttac catttttgaa
    25141 tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat aaaaccttaa aaatcgtctt
    25201 aacagctgca attagcgtta gtttaatcgg caatattgcg ttgttttggc agcttaaaga
    25261 agaacaagag acaaatacct ataaaattga agaagtggct aaaaacgcgt ctgataagct
    25321 caaaaaaaat tcttctgaag cacaggcgaa gattgacaag ttaaatcaaa ccatcaatga
    25381 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    25441 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    25501 aagcagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    25561 attaagtcct gtagacgatg atcaccaaca tgatgattac ggaagttact cttttacatt
    25621 aggtgacgtg caaacttacg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    25681 atttgttgat tatacaatcg gcaatcctca attcaaagat gtcaaaccac aaaaaataaa
    25741 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca cagactttag
    25801 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagcaaga caacgtttta
    25861 aaaaacgaaa aagttatctt agatggctgg ggatctttat gattctcggc tttttgattc
    25921 ttgctagtcc gatttacaca cataaaaagg tagacatacc agatgaagta agcacgaacg
    25981 catttttaaa cacgaaggaa ctgacgatgg tttcaaaaga aaaaggagcg aaccagcaat
    26041 tagtttttga atttgcgatc gacgatgaag atcagagctt attaaaagaa ctagctaatc
    26101 ttaattatcg tgtagaagta aagacagcta aaggggatta tcaagcaata aaagccaaag
    26161 tgatcaaggt gtctgatgat tattttgtag tgaagttagc acatgtacca gaagaatata
    26221 ttgctatgcg attaactatt attcctgaaa aaattgatcc aaaggtggat atgcaagaac
    26281 cgcaagatct gattttttat attcacgaag ataaggtcaa agatcacgtg aaagacgaag
    26341 attatgaaca acatgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    26401 cacaaaaaca aattgaatcc ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    26461 aactaaaaga tcaattgcct tatcaagtcg cagaagatca ggaaaatact gaaaataaga
    26521 ttaatagtta ccagcaagaa attgaaacat cgaaaacgca aatgaaagat caagaagaga
    26581 gcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaatatt tagaagaata
    26641 ggtggagtaa aagaaatgaa gcgagcgaac ttggtcggca aagatacttt cgctgagcag
    26701 aaagtattga ttaaagccta tctcaaagtt gtaaaagcta aagaaatatg tcaagaagtg
    26761 aattatctaa cacaacaata tctgagttga agaatggtag aaagagttta gataccttaa
    26821 aattcagatc atttgaagct ctatatatag ctgctaaaaa atattttaca gctgataata
    26881 aataaaaaat taaggtgggt tctatgtgaa aaaaacgtat caaaagtttt tatggattag
    26941 ctatatcgtc ctatttatcg ttgggtttct cgtcagtgtg tccgtgatcg tcggaacgct
    27001 ttttcttttg ttctctgacg aagatctggt acttaagggg ttaatgagta cgttatcgtg
    27061 tgtcatgtta ctttatttat gtatacagat caacgaaatt atgccccctc attggcaaga
    27121 aaagaagaaa gaatttaagg aagaaatcga aataaaatcc aatgactgga ttgaatacac
    27181 atggcttcct ctgaaaagaa atagcaaacg aaaggaattt tgggcattgc aattatggcc
    27241 aataggaaaa ctttttattt atatggcttc acttatggtt actttaatcg gttatttggt
    27301 ggtggtctat ggggtggcct attacttgtt aagtgagcca aaacagatcg cggcgtcaaa
    27361 tattttcacg gcagtaatga cattaatgtg tgtcatgtgc ttttctattt ttagttactt
    27421 actcgtccaa tttatcaggc aaaatttaac aatgagtaaa cttttcaaaa gtttattgga
    27481 cttacttatc ttaggcagta tgttaacgac tctaacagat cggttcgaac aatctgaaaa
    27541 aacggtagag gggataaaga acttcttaac gacgattgat caatttataa atggatatat
    27601 gtatttgttt gaagtaggct ttattctcac tttgtttggt tgtgtattta taaaactcat
    27661 taaaagatca atcgaaaaag aaatggttat tgatctaatt ccgacgctga gcggctatga
    27721 gtattacgtt gattttggaa agtatgagga gttgatccat acggatatcg ttcacacgag
    27781 tatccatgaa gtaaccgtta agggcatcag atctacttta aaagatggac aggagacgaa
    27841 aacaatcgac cgaagaaaag aggtctacaa tttttataaa gttgaagcaa gtctacgata
    27901 tggcattggg aaattgaggt tagatgggag taatattcaa caaaacaaac catatgtttt
    27961 aggaaaagtg gtgatttaga acttcctatg aaagatgaat aaataaggag aatcttttga
    28021 aaatgattct tctttacaga attgtgctta tagttggcac ggagttaaaa atcttaaaga
    28081 aggagcagtt attcaaagtg aaagaacatc ataatcagtt actatgtatt aaaacgggta
    28141 cgatgaacat tcaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
    28201 cgattcgaga gcaacataag caagaaaaat taatggctac ttatagccaa ttgattgatg
    28261 agtgggaaca attcaaggaa tctaaaacat aaaattccat ttaaacacct tattttttaa
    28321 agagcataag gaaacgtgta cccactttca aaataaagtg ggtagatagg ctgtgcctat
    28381 cacgtttctg aaaaaaataa atgaccccaa aattttcaat ttatgtgtag gtttatgggt
    28441 agcaaaaata tgaatgtggg tagcacaaaa gttcatttgt gggtagtaaa atttaaatat
    28501 gtgtagcaat gtgtgtagct acacacattg ctacacacgt tataacatat agtcaaaagg
    28561 agatatttat ggaagtaaaa ataagagata ttgatgaaaa aacgattcaa agaattgacc
    28621 gaagatgtat tgaattaagt gaacgtactg ggactaaatg gagccgaaat cgatacctaa
    28681 aagttttgat cgaaaatgat tttgatcacg ctcttctgga ttataaaaaa gatcaatttg
    28741 atcggttgtt agaaaaattt gtggatattc aaacttataa tacgaaaatt ttagaagaat
    28801 atattgcaac aaataatcaa ttaatcgcct tgttaattga acagtaaaaa taatattgtg
    28861 aatttataaa aggaggaaat caactttggc gaaagcaaca agaaaacata tttatcttta
    28921 tgatatgcat atcgaagtac ttgatgagat aatttctact catgacgata tatccaatta
    28981 tagtgaagca gttagacatt tgtgtatgat gtatgatccc aaaactgaag aaaagataag
    29041 tcccagtcaa atcaaactaa atgcaattgg aaaagaagtt tctattttaa ctgaaatggt
    29101 ttcggagttt gcagatcttc atttaaaaga tacaggaata ttaattggta caaattcaac
    29161 tgtttatcaa gaagcgaaag agcgtgtgga aggcaagata aaagcaaatc aaacgaagaa
    29221 atattctcct aagataaaga aaacaataag cgaatcagtg actacaaaaa gtgaagtatt
    29281 tgatccaaca aggtttttaa aacgataaaa attagaagga gaacgtgatg agtaaatcac
    29341 ctgcagtcat tctcacttgt caatttacct tgcccaataa aaaatctttt tcaacataca
    29401 ttgattatat gacaagagaa aaagcgctgg aagaaatgga acggcgcacg ctcgaagaag
    29461 aacaggaact gcaacgaatc aagaatgctt tagaggattt ttatattcct gaaggagaaa
    29521 ctttttcgga gagtaagacg gataaagaat tcagtgaaac gtcgaatgaa gcgcaatcgt
    29581 tgatggaata tggaatcaat tttgacaagt tagaggaaca agattttacc aaatatcttt
    29641 cttatatgac gcgtcactat gcgttagaac aaaaaaagga actaacggaa agggaaaaaa
    29701 tagaatatca acgtttgaat caagcaatgg aaaaatatca agcagaacca cacaaaaaaa
    29761 cttcaggaac tcttcctgga gttttttcta tatcttcaga cgaagtaaaa ggaagtgatc
    29821 tgaaggagat taagcagatc ttccaaagag ggcaacaaaa aggctcaatc atttatcaag
    29881 atgttgtttc tcatgataat tcgtatcttg aaaaaatagg attgtacgat cccaaaacag
    29941 atactttaga tgaagttgga ctaaaagctg ctggaaggaa aatgatggag acgttgtttg
    30001 agaaagaaaa actgaatgat acgggatatt ggatggctac cattcaccgt aacactaagc
    30061 atattcatat tcattttgca gttgttgaaa aagaaaatac gcgtaaacca tacactgagg
    30121 tcgaaaatgg tgtgagttat atcgtaccga aaggaaagag aagacaagca acattagacg
    30181 caatgaagac caattatgtg catgaattag aacgtttttc ttatgaaaag gatcgaatga
    30241 taggcatgga gaaacgaaat ttattaacgc ggaaaagtga tttgcgtaac agcttaacaa
    30301 aagtggtaaa agagccagta cgttatgatc gacacgcaat gaacttatta aatgaagtat
    30361 ataaaacctt acctgacaaa cgttcggaat ggaattatgg ggatgaaaat cgaactaaac
    30421 tttcaccata cacaagagag aaactcgacg agttaaccca atatgtgtta acaaaagaac
    30481 ctgaatacca agaatatata cagatttcaa aagcattgaa agaagaagca aaaagtcttt
    30541 atggggaaag caagagagaa tcaaaggatg cagagaaaaa tgctctcttt gatttaaaaa
    30601 agcgcacagg aaatgccatc ctaacagagc tgaagagaca ggatagtcaa ataaaaccat
    30661 taatagaaag tgttgatcag acgcaatcta tttacaatga acgatccttt cgaacgctta
    30721 aacaattgcc aacgaatgaa tacaaatata gttacgaaga attcatgaag cgcaggaaac
    30781 aattccaaca aagagttgtc acgcagcgga gtttgcgtca attaaatcga acgattaatg
    30841 cccatgaaga aaaataccac gcaagtaaag catacgaaga gcaacaacga agaatccaaa
    30901 tggaacaaga aagacagtaa agacagtaaa gacagtagga ggaatagcaa tgaaacaata
    30961 tttagatcaa tggaaagtta tagaaggatc attaagagaa gaacgaatcg aacaacttcc
    31021 cgattgttta gaaaaagagc atttgtttca gataagagaa atgttgataa acgaacagtt
    31081 tgatccaaat caatttttag tcgttgagta cccagctact ggtgtgtact gttgtaatca
    31141 tgtgaagggt gaaaaatatt ttatcattca agaatatgag ggcaagttag cgccttatta
    31201 tactacttgg gaaatgaatg aagaggggat caataacttc ccgtgtaaaa gcattgaaga
    31261 gtctatttca ctcacagaat gttagttgtt aagtgaataa aagaagtaaa agagtcctga
    31321 acgtgattgt ccaggactct tttttttcac tgaattgttt tccgccaatt cattgagtgg
    31381 tgcgatttcc gtctacatta tatcaaaaga aaggaggaag aagaaatggc ttattcaacg
    31441 attggagttg ttgttgaaaa aagcagagac aatcttgttt ttgtgacaga aattcaaaca
    31501 ggcagagctt ttgtagtcac agacaaagcg gcaaaagcat atcaaagtgg cgatatttta
    31561 acgttaaatc tgactacgaa gacgttcgtc gatgcggcag aagattatcc att
//
LOCUS       DADXTG010000020        29846 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.020, whole genome shotgun sequence.
ACCESSION   DADXTG010000020 DADXTG010000000
VERSION     DADXTG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.020"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="IWI57_000784"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="IWI57_000784"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBC2448947.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="IWI57_000785"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="IWI57_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBC2448948.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="IWI57_000786"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="IWI57_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBC2448949.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="IWI57_000787"
     CDS             2855..3730
                     /locus_tag="IWI57_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBC2448950.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IWI57_000788"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IWI57_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBC2448951.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="IWI57_000789"
     CDS             4627..5061
                     /locus_tag="IWI57_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBC2448952.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="IWI57_000790"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="IWI57_000790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBC2448953.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="IWI57_000791"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="IWI57_000791"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBC2448954.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="IWI57_000792"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="IWI57_000792"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBC2448955.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="IWI57_000793"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="IWI57_000793"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBC2448956.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="IWI57_000794"
     CDS             13332..14258
                     /locus_tag="IWI57_000794"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBC2448957.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15333
                     /locus_tag="IWI57_000795"
     CDS             14440..15333
                     /locus_tag="IWI57_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBC2448958.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15467..15592
                     /locus_tag="IWI57_000796"
     CDS             15467..15592
                     /locus_tag="IWI57_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2448959.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15726..16358)
                     /locus_tag="IWI57_000797"
     CDS             complement(15726..16358)
                     /locus_tag="IWI57_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448960.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16506..17048)
                     /locus_tag="IWI57_000798"
     CDS             complement(16506..17048)
                     /locus_tag="IWI57_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBC2448961.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17183..17572
                     /locus_tag="IWI57_000799"
     CDS             17183..17572
                     /locus_tag="IWI57_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448962.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17576..18340
                     /locus_tag="IWI57_000800"
     CDS             17576..18340
                     /locus_tag="IWI57_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBC2448963.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18469..19416
                     /locus_tag="IWI57_000801"
     CDS             18469..19416
                     /locus_tag="IWI57_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2448964.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19546..20286
                     /locus_tag="IWI57_000802"
     CDS             19546..20286
                     /locus_tag="IWI57_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448965.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(20577..21245)
                     /locus_tag="IWI57_000803"
     CDS             complement(20577..21245)
                     /locus_tag="IWI57_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2448966.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            21379..21666
                     /locus_tag="IWI57_000804"
     CDS             21379..21666
                     /locus_tag="IWI57_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2448967.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            22082..23620
                     /locus_tag="IWI57_000805"
     CDS             22082..23620
                     /locus_tag="IWI57_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBC2448968.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            23617..24582
                     /locus_tag="IWI57_000806"
     CDS             23617..24582
                     /locus_tag="IWI57_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448969.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            24569..25891
                     /locus_tag="IWI57_000807"
     CDS             24569..25891
                     /locus_tag="IWI57_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2448970.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      25954..26058
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            26132..26875
                     /locus_tag="IWI57_000808"
     CDS             26132..26875
                     /locus_tag="IWI57_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448971.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            26890..27882
                     /locus_tag="IWI57_000809"
     CDS             26890..27882
                     /locus_tag="IWI57_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2448972.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            27894..28631
                     /locus_tag="IWI57_000810"
     CDS             27894..28631
                     /locus_tag="IWI57_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448973.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            28621..29343
                     /locus_tag="IWI57_000811"
     CDS             28621..29343
                     /locus_tag="IWI57_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBC2448974.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(29427..>29846)
                     /locus_tag="IWI57_000812"
     CDS             complement(29427..>29846)
                     /locus_tag="IWI57_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBC2448975.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        10260 a         5088 c         6288 g         8210 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    14461 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    14521 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    14581 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    14641 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    14701 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    14761 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    14821 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    14881 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    14941 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15001 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15061 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15121 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15181 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15241 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15301 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15361 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15421 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    15481 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    15541 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    15601 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    15661 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    15721 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    15781 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    15841 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    15901 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    15961 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16021 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16081 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16141 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16201 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16261 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16321 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    16381 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    16441 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    16501 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    16561 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    16621 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    16681 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    16741 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    16801 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    16861 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    16921 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    16981 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    17041 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    17101 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    17161 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    17221 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    17281 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    17341 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    17401 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    17461 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    17521 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    17581 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    17641 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    17701 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    17761 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    17821 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    17881 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    17941 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    18001 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    18061 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    18121 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    18181 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    18241 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    18301 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    18361 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    18421 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    18481 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    18541 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    18601 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    18661 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    18721 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    18781 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    18841 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    18901 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    18961 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    19021 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    19081 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    19141 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    19201 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    19261 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    19321 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    19381 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatattta
    19441 tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct
    19501 gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga
    19561 cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac
    19621 aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg
    19681 tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag
    19741 cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa
    19801 ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa
    19861 aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag
    19921 catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct
    19981 aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat
    20041 ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg
    20101 ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg
    20161 ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa
    20221 cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt
    20281 aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt
    20341 ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag
    20401 cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactattttc
    20461 gcagatcgtt tgcaaaaata gtttattttt ttataaagaa gattttactg aataatatca
    20521 ttttagttta cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag
    20581 tgctctaact gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc
    20641 aattggggca aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga
    20701 atagaacagc taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca
    20761 actaaaatac caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt
    20821 tcaatggctt gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga
    20881 ccgcaaccaa tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca
    20941 gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg
    21001 acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt
    21061 aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa
    21121 gcaataccag taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct
    21181 aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt
    21241 ttcatttcta gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta
    21301 tactcttact tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat
    21361 caaataagga gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc
    21421 aaaaaaacag agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca
    21481 atggtccaga taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca
    21541 cttgctatga aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat
    21601 cagtcaaaag aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg
    21661 atataagtaa aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt
    21721 tttggcaaaa aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt
    21781 ctcccccacg agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg
    21841 tgtaaaaaag atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt
    21901 agtgaaaaaa cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc
    21961 agcatattta gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca
    22021 agttagttgg gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg
    22081 catgaataat tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt
    22141 tcaacatctg tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt
    22201 agcgatttgg gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt
    22261 gctccaaacg attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg
    22321 aactgttcca gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac
    22381 gtatattggt ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat
    22441 gtccagaatg agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc
    22501 aggtatccga actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg
    22561 agcaggtgga ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac
    22621 attgattggt gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg
    22681 gttgcagcat aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt
    22741 tggcggcgtt tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg
    22801 aaaattagga gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa
    22861 cacaaaaaca gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc
    22921 actggatcaa ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt
    22981 ggtgaaggta cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga
    23041 agcagaagaa ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca
    23101 aaatacgtat gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat
    23161 ttccgatttg gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga
    23221 tcgttcagat ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca
    23281 aagcatggag ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga
    23341 tgcctattcc acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg
    23401 agggttattc ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca
    23461 tcctgaaatc gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat
    23521 tgatatgaat taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt
    23581 cctgaaaaat gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa
    23641 catgtgcaaa aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa
    23701 aaaggagaaa tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa
    23761 atgatcaatg gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta
    23821 aatgaatata atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct
    23881 ttgttcccga ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg
    23941 gaaaagcaga aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct
    24001 gatatttatc gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg
    24061 atcatccgag ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca
    24121 ttagatccga tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg
    24181 ggcacgacca tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt
    24241 attgcattta tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg
    24301 aatcctaaaa acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg
    24361 cgagtagaag atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt
    24421 caacaagttt gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca
    24481 agcattgtga tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt
    24541 gcttttttaa gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc
    24601 aggtgtgagc ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt
    24661 ggtcgaagaa gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt
    24721 cttgttaagt gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa
    24781 cgagatccca actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc
    24841 agtcccagaa agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac
    24901 cgctcgtttt ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta
    24961 cttagttttg gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt
    25021 gaaaactagt gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg
    25081 tggtggttat attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac
    25141 cattgtccat cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc
    25201 cgtccatcaa atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat
    25261 catttcagaa ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat
    25321 gattttttgt gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa
    25381 tatcgtcttt gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa
    25441 aatttttgcg tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac
    25501 ttttgaagga aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata
    25561 tccgattata cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa
    25621 aagtcacgcc tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta
    25681 tcatcgtgtc aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt
    25741 gattggtgct gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt
    25801 catcaatcaa aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt
    25861 ggcaagtgat ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct
    25921 ttttccttct tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg
    25981 acatgagagt aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg
    26041 agaaacatag cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg
    26101 ttattcatat tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag
    26161 tatggcattc attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt
    26221 tattttaccc ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact
    26281 ttcccattct gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct
    26341 ttcttttatt actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc
    26401 gttactagtg atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg
    26461 gtttggtttc ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat
    26521 cgtagtttct ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa
    26581 aacgatgggg gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc
    26641 cggcttcttt tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg
    26701 agaatggcta agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta
    26761 ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa
    26821 tggcgcttgt tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa
    26881 ggggatcaaa tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt
    26941 ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt
    27001 gttttggatt atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc
    27061 tattataaag aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt
    27121 attggtctag taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact
    27181 tatgctcacg cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat
    27241 aatacttctg gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa
    27301 ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg
    27361 gaaaaagacg gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca
    27421 gaaagcttag gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa
    27481 gcaaaagaag cgggtattga actaaactat atcccattga aagaattaga tgaacggtta
    27541 gattactata ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg
    27601 gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat
    27661 gacagtgcta aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa
    27721 tcacaagcat tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa
    27781 gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat
    27841 gtaccttcgc aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa
    27901 tccaaatcag ccacttgaat gtagtcattg aaaacctgaa agtattagaa gacatccatt
    27961 tcactatccc taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa
    28021 cattgctgaa aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg
    28081 atcaaaaagt aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac
    28141 ttccttggct gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa
    28201 tcaatgaaga acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt
    28261 ttttgccgga acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca
    28321 tgaatccttc ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca
    28381 gagggcagat gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac
    28441 ttgtgacgca tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg
    28501 cccgtccggc acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat
    28561 ggctactgca gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag
    28621 atgttaagtg atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag
    28681 gaacacacga ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga
    28741 cagcttgcag caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat
    28801 agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata
    28861 gggttagata atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga
    28921 cagcttgctt ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa
    28981 aaagagatct taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct
    29041 tctttggact atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat
    29101 ttgaaaaaga atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt
    29161 attgaaacgg atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa
    29221 gattatgtct cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca
    29281 gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa
    29341 taaaaaagag atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag
    29401 taaactgttt gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc
    29461 gccgcttccc aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt
    29521 gcagcagagt agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt
    29581 ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag
    29641 cttgtgcttg ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca
    29701 acaggcgctg cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg
    29761 actggtgctt cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt
    29821 gggattgtta attgttgtcc tgagta
//
LOCUS       DADXTG010000021        29233 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.021, whole genome shotgun sequence.
ACCESSION   DADXTG010000021 DADXTG010000000
VERSION     DADXTG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.021"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(111..1160)
                     /locus_tag="IWI57_000813"
     CDS             complement(111..1160)
                     /locus_tag="IWI57_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2448976.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1242..1931)
                     /locus_tag="IWI57_000814"
     CDS             complement(1242..1931)
                     /locus_tag="IWI57_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBC2448977.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2037..2405)
                     /gene="rplL"
                     /locus_tag="IWI57_000815"
     CDS             complement(2037..2405)
                     /gene="rplL"
                     /locus_tag="IWI57_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBC2448978.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2458..2961)
                     /locus_tag="IWI57_000816"
     CDS             complement(2458..2961)
                     /locus_tag="IWI57_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBC2448979.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3165..3292)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3371..4060)
                     /gene="rplA"
                     /locus_tag="IWI57_000817"
     CDS             complement(3371..4060)
                     /gene="rplA"
                     /locus_tag="IWI57_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBC2448980.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4164..4586)
                     /gene="rplK"
                     /locus_tag="IWI57_000818"
     CDS             complement(4164..4586)
                     /gene="rplK"
                     /locus_tag="IWI57_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBC2448981.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4929..5597
                     /gene="sdaAB"
                     /locus_tag="IWI57_000819"
     CDS             4929..5597
                     /gene="sdaAB"
                     /locus_tag="IWI57_000819"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBC2448982.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5610..6482
                     /gene="sdaAA"
                     /locus_tag="IWI57_000820"
     CDS             5610..6482
                     /gene="sdaAA"
                     /locus_tag="IWI57_000820"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBC2448983.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6573..7592)
                     /locus_tag="IWI57_000821"
     CDS             complement(6573..7592)
                     /locus_tag="IWI57_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBC2448984.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7765..8670)
                     /locus_tag="IWI57_000822"
     CDS             complement(7765..8670)
                     /locus_tag="IWI57_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBC2448985.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8729..9274)
                     /gene="nusG"
                     /locus_tag="IWI57_000823"
     CDS             complement(8729..9274)
                     /gene="nusG"
                     /locus_tag="IWI57_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBC2448986.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9375..9545)
                     /gene="secE"
                     /locus_tag="IWI57_000824"
     CDS             complement(9375..9545)
                     /gene="secE"
                     /locus_tag="IWI57_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBC2448987.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9569..9721)
                     /gene="rpmG"
                     /locus_tag="IWI57_000825"
     CDS             complement(9569..9721)
                     /gene="rpmG"
                     /locus_tag="IWI57_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBC2448988.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9883..10527
                     /locus_tag="IWI57_000826"
     CDS             9883..10527
                     /locus_tag="IWI57_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2448989.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10583..11086)
                     /locus_tag="IWI57_000827"
     CDS             complement(10583..11086)
                     /locus_tag="IWI57_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBC2448990.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11099..11145)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11683..12648
                     /locus_tag="IWI57_000828"
     CDS             11683..12648
                     /locus_tag="IWI57_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBC2448991.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12747..13919)
                     /locus_tag="IWI57_000829"
     CDS             complement(12747..13919)
                     /locus_tag="IWI57_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448992.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13906..15051)
                     /locus_tag="IWI57_000830"
     CDS             complement(13906..15051)
                     /locus_tag="IWI57_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2448993.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15065..15997)
                     /locus_tag="IWI57_000831"
     CDS             complement(15065..15997)
                     /locus_tag="IWI57_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2448994.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16114..17193
                     /locus_tag="IWI57_000832"
     CDS             16114..17193
                     /locus_tag="IWI57_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBC2448995.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17190..17819
                     /locus_tag="IWI57_000833"
     CDS             17190..17819
                     /locus_tag="IWI57_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2448996.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17929..18156)
                     /locus_tag="IWI57_000834"
     CDS             complement(17929..18156)
                     /locus_tag="IWI57_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2448997.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18259..20847)
                     /locus_tag="IWI57_000835"
     CDS             complement(18259..20847)
                     /locus_tag="IWI57_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBC2448998.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20867..21526)
                     /locus_tag="IWI57_000836"
     CDS             complement(20867..21526)
                     /locus_tag="IWI57_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBC2448999.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21575..22078)
                     /gene="trmL"
                     /locus_tag="IWI57_000837"
     CDS             complement(21575..22078)
                     /gene="trmL"
                     /locus_tag="IWI57_000837"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBC2449000.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22299..23165)
                     /locus_tag="IWI57_000838"
     CDS             complement(22299..23165)
                     /locus_tag="IWI57_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBC2449001.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23274..23915
                     /locus_tag="IWI57_000839"
     CDS             23274..23915
                     /locus_tag="IWI57_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBC2449002.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23977..25338)
                     /gene="mgtE"
                     /locus_tag="IWI57_000840"
     CDS             complement(23977..25338)
                     /gene="mgtE"
                     /locus_tag="IWI57_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBC2449003.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25356..26255)
                     /locus_tag="IWI57_000841"
     CDS             complement(25356..26255)
                     /locus_tag="IWI57_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBC2449004.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26257..27054)
                     /locus_tag="IWI57_000842"
     CDS             complement(26257..27054)
                     /locus_tag="IWI57_000842"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBC2449005.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27032..27736)
                     /locus_tag="IWI57_000843"
     CDS             complement(27032..27736)
                     /locus_tag="IWI57_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBC2449006.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27867..28442
                     /locus_tag="IWI57_000844"
     CDS             27867..28442
                     /locus_tag="IWI57_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBC2449007.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28560..>29233
                     /locus_tag="IWI57_000845"
     CDS             28560..>29233
                     /locus_tag="IWI57_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBC2449008.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8597 a         6190 c         5255 g         9191 t
ORIGIN      
        1 tattttgatg aacgcatatg tagtcctcat ttcttgggag ttttgagact actatatgcg
       61 tttttttgtt gatttaccaa catgtttaga cattttcctc taggaaaaaa ttaactagca
      121 ccacgggtaa atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt
      181 gtttgattgg cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc
      241 aaaatggcca aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca
      301 agtcccatat acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt
      361 gcagcaactg ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa
      421 ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca
      481 aacatgaaaa atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc
      541 ggcaaacttt taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc
      601 ggcattttcc agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag
      661 atcatccgcc aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc
      721 cccacgagta aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct
      781 agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa
      841 gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca
      901 gataacgcta ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact
      961 aaaaacagta accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca
     1021 cttgtgctaa cagataaact agctgctact tttgttccaa tcggctggat gtaatacata
     1081 ttggcaacaa caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt
     1141 gttttttcat tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga
     1201 aaagattgga aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt
     1261 ccttgttttt tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca
     1321 cttgcaacca acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac
     1381 gcatacaaca cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga
     1441 ggcaatttca tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata
     1501 tagcctaaca ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga
     1561 ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg
     1621 ttaccaagag cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa
     1681 ccagcaatga ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc
     1741 aatacgatca agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct
     1801 gccttactcg ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac
     1861 cgatagccca ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt
     1921 ttctttttca tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa
     1981 aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat
     2041 ttaacagtta cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct
     2101 ttagatacgc cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc
     2161 aagcctaaac cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct
     2221 gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca
     2281 gctacagcta caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg
     2341 tcgttaagtt ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat
     2401 gccattataa tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta
     2461 agctgcatct ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac
     2521 tggcgcttgt agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc
     2581 caatgctgtg atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc
     2641 taatgctttt gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc
     2701 gttactgaat gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc
     2761 ttttttagct gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc
     2821 acgaagttgt ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac
     2881 tactactgat gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt
     2941 tgcgataatt gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt
     3001 ttgcactgaa aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac
     3061 ctattcactt cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt
     3121 tgctcaacca gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa
     3181 ctctatgcca ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag
     3241 gaaattaagg cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta
     3301 gcttagcatg ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc
     3361 taatatttaa ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga
     3421 tcgttacatt tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat
     3481 catggattgc tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg
     3541 gtacatggat atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt
     3601 ttactgcttt tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt
     3661 ttggtcctaa tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa
     3721 ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga
     3781 aatctgcacc agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag
     3841 tttgtgtttt accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt
     3901 ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt
     3961 ttgtttcttt tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt
     4021 tttttaatgc atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt
     4081 ggttataacg gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa
     4141 gctgctgtct tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct
     4201 tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca
     4261 gcgatttctc ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct
     4321 gaaccttttt cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta
     4381 acgaatgtga atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct
     4441 tgatcagctg tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga
     4501 cctagtgcag gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta
     4561 acgatttttt ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa
     4621 ttggagatgc atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa
     4681 tgtgcaaaac aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt
     4741 ttctgctatt tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat
     4801 ttttcgttct catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa
     4861 gcaaacaata acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg
     4921 tggagaaaat gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga
     4981 ttggtccaag tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta
     5041 tatttggcga acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct
     5101 atcgggggca cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg
     5161 atgaacgttt agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg
     5221 ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa
     5281 cacgaaagct ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat
     5341 tgaatggatt caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag
     5401 atgttccagg tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg
     5461 gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag
     5521 atgatcctca agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg
     5581 taaattattt cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc
     5641 aagcaaaagc ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg
     5701 gccgttcccg tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat
     5761 caatcaacga gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa
     5821 aaaaattaga tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag
     5881 ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta
     5941 caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc
     6001 ttgatttgac acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag
     6061 tcatcgcaaa caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt
     6121 ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag
     6181 cagcacaagc agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg
     6241 caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta
     6301 tctctgctga tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg
     6361 ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg
     6421 gcttagctgt tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt
     6481 gataaagctc cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag
     6541 agaggactaa ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact
     6601 tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt
     6661 tcaaaaacag atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg
     6721 ccaatcgcaa ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg
     6781 acaccagagt cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat
     6841 acttcaaagg acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata
     6901 ccatctgccc ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg
     6961 ataactggaa gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt
     7021 tttgcagctg cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt
     7081 tcgctatacg cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct
     7141 tcttcacggt aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct
     7201 tctactgctt tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg
     7261 aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa
     7321 gtgctgatgg aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct
     7381 ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca
     7441 attccgaaaa cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg
     7501 attttctttg tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca
     7561 ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag
     7621 gcaatgttta caatatcgat tggatgttct tcattacttg tatgataagt catagtagat
     7681 ccttctttca tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac
     7741 atttactctc agatactttt atcgctattc tttccaaata aatcgactta caagctgatc
     7801 tacttcttgg ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc
     7861 ctctatttca tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg
     7921 tactgttcca tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt
     7981 ggttatagga atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc
     8041 aggccccttt gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa
     8101 aggcgcgcca attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt
     8161 tcctaaatag cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc
     8221 tatgccgtag ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca
     8281 ctcgttattt ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga
     8341 caaatctcct gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac
     8401 tgcttgctga tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct
     8461 gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc
     8521 cgttttttct ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa
     8581 cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat
     8641 tggttctgtc atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat
     8701 catatgatcg acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa
     8761 gttctgtact ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc
     8821 cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga
     8881 cttcgccaac ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga
     8941 tatgattgac ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa
     9001 cgaatcctgt cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca
     9061 tttcaacaag tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt
     9121 tgacttcagt ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca
     9181 tactttgcgc acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat
     9241 gcaagacata ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa
     9301 taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg
     9361 ttgtggctag cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt
     9421 acgtaaaaca tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg
     9481 agttgttttc tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat
     9541 ttcatgagac aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat
     9601 tacaatattt acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg
     9661 attttgtgta gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca
     9721 tttacttctg cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt
     9781 tttacttgtc aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt
     9841 aatttgaaat taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt
     9901 gtatataaga aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta
     9961 aaaattttag aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt
    10021 ttccgtggaa acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg
    10081 acgcttcctg tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct
    10141 ttcttcaaag tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa
    10201 aatcattttt atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg
    10261 agcgtagaac aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg
    10321 gctgctattg caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat
    10381 atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg
    10441 aaagtatgtg aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt
    10501 gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc
    10561 tttctttttt cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat
    10621 aatacatcaa tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat
    10681 aggttgtcca aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc
    10741 tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt
    10801 gcaataaagc tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca
    10861 tgataccaaa accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca
    10921 atttacggtt aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc
    10981 ctgaggatac aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat
    11041 ttacaacgat tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt
    11101 tctcctagtt tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata
    11161 attataacag acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc
    11221 ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg
    11281 tcataagaat tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa
    11341 acttaaataa ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta
    11401 gcaagcagct gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta
    11461 atggatcatg cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt
    11521 cctagacttc tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca
    11581 agtttctttc attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg
    11641 agtattaaaa tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat
    11701 atattgatca atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta
    11761 ccggtattaa aaaataagca tattgactat acacttcatt atagtgaata caaaggacac
    11821 gatgctgaga ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa
    11881 cagaaaacaa tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat
    11941 caggtacttg atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca
    12001 ggttctggaa atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt
    12061 cagctgattt tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa
    12121 ataagtgaaa aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc
    12181 gtccatgcga caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca
    12241 ttttcgtatt tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg
    12301 gtagatgcag gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag
    12361 catccttttt ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc
    12421 attgattttg tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta
    12481 cttatacaga aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta
    12541 cgtatcgttt caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga
    12601 ccttttgata tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt
    12661 tgtcagaagc tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt
    12721 gtcacagtcc tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag
    12781 tttctttctt tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag
    12841 acacaaccat tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg
    12901 atcaagttat tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc
    12961 atttctggca agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata
    13021 aaaatatctt tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc
    13081 gaaaagctaa tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt
    13141 atatcccacg aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac
    13201 agcagcgagt aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg
    13261 ggcgcactct ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg
    13321 aaaccgctaa aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc
    13381 aacagctttt tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag
    13441 agtgccattt gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga
    13501 atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct
    13561 gggcgactct gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct
    13621 gcaggcagat gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt
    13681 tgtgaggttc catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca
    13741 tcgactacag gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta
    13801 ttttcaacat caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct
    13861 atcagtaaat tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata
    13921 acgcacccct ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa
    13981 ccatagaaga ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact
    14041 ttcgctgatc aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca
    14101 atacttcact tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga
    14161 aatcaagatg ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt
    14221 acctaacaaa atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg
    14281 ttcaccaaag tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt
    14341 caaaacaatc gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg
    14401 aatcaaggac aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg
    14461 gaccccccac atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact
    14521 tttgaaactc atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac
    14581 agtcatgatg ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc
    14641 aatacgctgt aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg
    14701 acccacatgg agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc
    14761 atttagcttt tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc
    14821 attgacttcg acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc
    14881 tttgattaca attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa
    14941 ggccgccata aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga
    15001 tttatttcgc agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc
    15061 tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt
    15121 ctttggttgc tatttttccg taactgatct tttcttcttg taagattgta agaatcggca
    15181 acaatactgt atttagttca gtagttgtta cttggagctg acctttttta taagcgattc
    15241 catcagaagc tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt
    15301 cgatcgtcaa acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag
    15361 caatcacctg tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca
    15421 tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta
    15481 aaattttatt ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc
    15541 gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt
    15601 ctcctggacg aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca
    15661 aggctttcct tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt
    15721 gcacacttag ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga
    15781 cagcataatt ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat
    15841 ttaacaaaga aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca
    15901 gacctaagac ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc
    15961 catagcgttt gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt
    16021 cttgttgatc tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca
    16081 cacatgacat ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa
    16141 actagctttc tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg
    16201 caagttatct atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca
    16261 aagccaaatt ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt
    16321 gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac
    16381 ttgtttttgt taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc
    16441 gcctttgtcg taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg
    16501 accgatcttg agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag
    16561 ttatcggaac aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt
    16621 gtggaagaac ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca
    16681 agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa
    16741 ccattagaaa atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc
    16801 catgacctcc atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat
    16861 tttccttctt ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa
    16921 aactgcttct taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc
    16981 aaaaccgttg ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat
    17041 gatggcacca gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa
    17101 cgtgttcaag aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta
    17161 acgatcattt taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc
    17221 catttgtgac gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag
    17281 gcattggtca ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt
    17341 tgctgacgga catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg
    17401 ctaaatttcc caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc
    17461 gcgaagcatt ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta
    17521 ttccttctgt caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga
    17581 ctttcactca aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta
    17641 gtgaacgtga gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg
    17701 cagatgcctt gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa
    17761 agctagattt acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag
    17821 tcttatctaa cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac
    17881 atcttctatt caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc
    17941 agggctgcca ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc
    18001 taacgttttt tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt
    18061 caatttgaat tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc
    18121 taatttcgct aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt
    18181 cagcttagca tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc
    18241 tatttctcat ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt
    18301 ttccatacca atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa
    18361 atctttagaa acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa
    18421 cagatctggt tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc
    18481 ttctacaggt agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa
    18541 tggatcattt ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt
    18601 cattttatca ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt
    18661 cgctacacaa gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg
    18721 agtcactgcc gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg
    18781 caaaataacc atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag
    18841 agtgatttta ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac
    18901 taattcatcc actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat
    18961 atctccattg aatacattca attcgggagt attgactaat tggaggactt tgtctcctat
    19021 ccgataaacg gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat
    19081 ctcctgcatc attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa
    19141 tacttggata tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat
    19201 caagggttca atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt
    19261 gaaatctttt ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc
    19321 atcaccttgc cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag
    19381 atcatggagt acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat
    19441 aacttgcata ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat
    19501 agacatctca tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt
    19561 tttttcccgt ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt
    19621 ttcgttcatt cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc
    19681 ttgggtatat tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat
    19741 tccgttgatc acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga
    19801 acggattgct tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt
    19861 ttcgatacgc cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg
    19921 actgagaagg cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc
    19981 gtaaagatta gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt
    20041 agcaacctga tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg
    20101 ctgaagcaat gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat
    20161 ctgatgcaag atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc
    20221 ggctagatta tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg
    20281 attttcttcg atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa
    20341 ctgactaccg aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag
    20401 ccgaatcgtc tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa
    20461 taccgaagga tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt
    20521 ttctgctgtt ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc
    20581 atttgcagtt gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt
    20641 aggatgatcc actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa
    20701 acttccggtg actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac
    20761 caaaagcact ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac
    20821 gaaataaggc atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt
    20881 ctggtgctgt ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca
    20941 ataatctgca aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca
    21001 tttcacgcaa ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag
    21061 ctccatgtcc tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa
    21121 cagtttcgat acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat
    21181 acagatctaa atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat
    21241 cgatcgactg accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa
    21301 tctctaccgg acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg
    21361 ctgtactgga ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga
    21421 tttcttcgta actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt
    21481 cctgattcca ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc
    21541 tgtctttctt ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt
    21601 gatgcgtcaa ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg
    21661 cagctgtatt cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac
    21721 acttttcttc gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata
    21781 aaaagaagtg ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt
    21841 ttgagatcaa atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt
    21901 ggtaagtaat atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat
    21961 cagttgaaaa acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac
    22021 gggcaatatt tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca
    22081 ggatcattcc tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa
    22141 aaaaaaacgt atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct
    22201 cactaacttt tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta
    22261 atataacacc ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt
    22321 tttctcttag ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta
    22381 tataagtctg ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga
    22441 tctttttgat tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat
    22501 ttcattgctt cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt
    22561 tgattcggat aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc
    22621 aacacttctc catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag
    22681 atatttaaaa ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca
    22741 caccaaaatg cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca
    22801 aatccaacag cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct
    22861 ccgcagccaa catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata
    22921 acgggctgat acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg
    22981 tattctgttt taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa
    23041 cggtgattat ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc
    23101 ggacagcgga acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt
    23161 aacatagttc cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc
    23221 taaaaatatg atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca
    23281 aagaattttg cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc
    23341 ggatcgaatt gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg
    23401 aagaaacgtt aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta
    23461 gagggggcga cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg
    23521 aagcaaagaa attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc
    23581 tagacgaaga ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc
    23641 atatggcttt tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag
    23701 aacatggtgt tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa
    23761 atttcccacg gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg
    23821 gtggtggtgt cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag
    23881 tacacggaac caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca
    23941 acaggcataa tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa
    24001 agaagcaata ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg
    24061 accgcttgct actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc
    24121 tgcaagattc gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat
    24181 aaaattatgt ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt
    24241 gacaacgccg gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc
    24301 agaaatcgca agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt
    24361 tcctgtaatc agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata
    24421 gtgactgatt aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa
    24481 acgtcgagac gctgccttta ctggattttc attaatttct tccacattga ccccggcaag
    24541 tcctgaataa tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac
    24601 gattcctaac aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct
    24661 aaatgtttgt gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct
    24721 catcagatca gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac
    24781 ccctactagc tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc
    24841 tgcttcgttc ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac
    24901 aaattcagtt gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat
    24961 ttcactggca tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag
    25021 cgtattaagc aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata
    25081 gctaggacgc atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc
    25141 gaacatatcc gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc
    25201 tgtaagagat tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct
    25261 gaatgcctga atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa
    25321 ttcttgattt tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact
    25381 gcctccatgt ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg
    25441 taaaaacgca gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg
    25501 ccatacatat catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga
    25561 tgtgtccttc ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct
    25621 atccagtact cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa
    25681 gaataatctc gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt
    25741 ccactgacta aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat
    25801 tttgcgtgcg caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt
    25861 cgcgtcacca catggattac ttggttttcg taaccttgtt ttttataata agcctttaca
    25921 cgattagcca ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt
    25981 ttatttacta ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa
    26041 agtacggttt catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct
    26101 agagaaaaca agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt
    26161 attttcgcta acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga
    26221 gaatgatctt tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc
    26281 tttcacacga tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc
    26341 tattcgataa taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac
    26401 aagataatct tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat
    26461 cggagaaccc aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa
    26521 ggcattgata ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc
    26581 agtaggcgta gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat
    26641 gtatacatcg gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa
    26701 aaaatgctgg tccggtgttt catccaaata actgatccga acatccagca agggataact
    26761 aacactttgt tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg
    26821 ccaatccgtg taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag
    26881 acaatgactg tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac
    26941 caattctggc tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg
    27001 ttctgtcact tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg
    27061 cctcctctct ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc
    27121 ctcttccatg ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct
    27181 cttcatccaa ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg
    27241 gaaaaacgcc ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg
    27301 caagagtccg aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag
    27361 gatactcgat caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat
    27421 ctcgttcttg gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga
    27481 tatcttctac aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta
    27541 aacgtcccat tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga
    27601 ctctcccagt gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga
    27661 tcccccgcag tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt
    27721 cccaatctcg ttccatagct gatacctcta cttcattaac attcattgtt tattctacca
    27781 tatatgcaaa gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc
    27841 aaaatagaca aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta
    27901 tgctgacaaa gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt
    27961 tccgtgtaca gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt
    28021 gtggcctacg gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg
    28081 aaaaagggcg tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct
    28141 tcgaaaaacg tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg
    28201 atcctgcttc attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg
    28261 aagaaggact tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg
    28321 aactagaggt aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt
    28381 tttctttacc ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt
    28441 agaaaccttt cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt
    28501 ataggatgtg aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata
    28561 tgatcgaaat ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac
    28621 agttattaca atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc
    28681 gctttcttcc tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt
    28741 ctcagaacaa tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg
    28801 actgcaaagc ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc
    28861 aagaagcagt cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg
    28921 aagaaatcgg cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag
    28981 aagtattctt ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg
    29041 cagtcgttta caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta
    29101 caagtagcct ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt
    29161 ttaatggtga acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac
    29221 acttgctttc taa
//
LOCUS       DADXTG010000022        27843 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.022, whole genome shotgun sequence.
ACCESSION   DADXTG010000022 DADXTG010000000
VERSION     DADXTG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.022"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            183..1442
                     /gene="lysA"
                     /locus_tag="IWI57_000846"
     CDS             183..1442
                     /gene="lysA"
                     /locus_tag="IWI57_000846"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBC2449009.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1429..2229
                     /gene="dapF"
                     /locus_tag="IWI57_000847"
     CDS             1429..2229
                     /gene="dapF"
                     /locus_tag="IWI57_000847"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBC2449010.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2232..3113
                     /locus_tag="IWI57_000848"
     CDS             2232..3113
                     /locus_tag="IWI57_000848"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBC2449011.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3110..3826
                     /locus_tag="IWI57_000849"
     CDS             3110..3826
                     /locus_tag="IWI57_000849"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBC2449012.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3832..4527
                     /gene="dapD"
                     /locus_tag="IWI57_000850"
     CDS             3832..4527
                     /gene="dapD"
                     /locus_tag="IWI57_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBC2449013.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4543..5544
                     /locus_tag="IWI57_000851"
     CDS             4543..5544
                     /locus_tag="IWI57_000851"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBC2449014.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5541..6902
                     /locus_tag="IWI57_000852"
     CDS             5541..6902
                     /locus_tag="IWI57_000852"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBC2449015.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6862..8013
                     /locus_tag="IWI57_000853"
     CDS             6862..8013
                     /locus_tag="IWI57_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBC2449016.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7988..9157
                     /locus_tag="IWI57_000854"
     CDS             7988..9157
                     /locus_tag="IWI57_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBC2449017.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9332..10711
                     /locus_tag="IWI57_000855"
     CDS             9332..10711
                     /locus_tag="IWI57_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBC2449018.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10992..11789
                     /locus_tag="IWI57_000856"
                     /pseudo
     CDS             10992..11789
                     /locus_tag="IWI57_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11978..12646
                     /locus_tag="IWI57_000857"
     CDS             11978..12646
                     /locus_tag="IWI57_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBC2449019.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12846..13232)
                     /locus_tag="IWI57_000858"
     CDS             complement(12846..13232)
                     /locus_tag="IWI57_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449020.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13204..14586)
                     /locus_tag="IWI57_000859"
     CDS             complement(13204..14586)
                     /locus_tag="IWI57_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBC2449021.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14742..15929)
                     /locus_tag="IWI57_000860"
     CDS             complement(14742..15929)
                     /locus_tag="IWI57_000860"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBC2449022.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15936..16967)
                     /locus_tag="IWI57_000861"
     CDS             complement(15936..16967)
                     /locus_tag="IWI57_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBC2449023.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16983..18437)
                     /locus_tag="IWI57_000862"
     CDS             complement(16983..18437)
                     /locus_tag="IWI57_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2449024.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            18612..19517
                     /locus_tag="IWI57_000863"
     CDS             18612..19517
                     /locus_tag="IWI57_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBC2449025.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19608..20285
                     /locus_tag="IWI57_000864"
     CDS             19608..20285
                     /locus_tag="IWI57_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBC2449026.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20282..20452
                     /locus_tag="IWI57_000865"
     CDS             20282..20452
                     /locus_tag="IWI57_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449027.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20566..21462
                     /locus_tag="IWI57_000866"
     CDS             20566..21462
                     /locus_tag="IWI57_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2449028.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21517..22887)
                     /locus_tag="IWI57_000867"
     CDS             complement(21517..22887)
                     /locus_tag="IWI57_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2449029.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23125..23871
                     /locus_tag="IWI57_000868"
     CDS             23125..23871
                     /locus_tag="IWI57_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBC2449030.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23868..24707
                     /locus_tag="IWI57_000869"
     CDS             23868..24707
                     /locus_tag="IWI57_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBC2449031.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24708..25658
                     /locus_tag="IWI57_000870"
     CDS             24708..25658
                     /locus_tag="IWI57_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBC2449032.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25799..26551
                     /locus_tag="IWI57_000871"
     CDS             25799..26551
                     /locus_tag="IWI57_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449033.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26570..27721
                     /gene="queG"
                     /locus_tag="IWI57_000872"
     CDS             26570..27721
                     /gene="queG"
                     /locus_tag="IWI57_000872"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBC2449034.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9137 a         5057 c         5840 g         7809 t
ORIGIN      
        1 agcttctaat agttcgggta ttttgttttt ataaccattt ttattcttat ttttaaaaaa
       61 atatttcgaa atatactaga caattcagaa aattttgttt aatattattc caataaaatt
      121 caaatcttta tcaaatgaaa ttccaataaa aacggaaaaa aagtgaaaag aggaatgaaa
      181 aaatgacaga attgataatt ggcggagtca cagcaagcac attggcagaa aagtataaga
      241 cacctctgta tgtgtatgat gaagaaaaga tgagacaaac gatgctgtca ttcaagcgag
      301 gctttgtatc gaaacgtttt gatacaaaag tgctttacgc ctctaaagca ttccaaacga
      361 ttgagatgct gcatttagtt cgtgaatatg gattcgggct agatgttgtc agtggtgggg
      421 aaatctatac cgctttgcaa gctgattttc ctagagatac tatctacttc catggtaaca
      481 acaagacgcc agaggaactg atctatgcac ttgaaaatga tttgttgcat attgttgccg
      541 ataatttgat ggaagttgaa ttattagcag aattaagcca acgctattta aaaaaaatct
      601 gtatcatgct tcggttgaat gtcggagtag aagcacatac tcatgaatat atcgtcactg
      661 cgcatattga ttcaaaattc ggcatgagct atgaaagtaa agagtgccaa caagcccttc
      721 agttgataaa agaaagcagc tatctagagt tagaaggatt tcatgctcat atcgggtcac
      781 aaatttttga tgttactgct tggttagctg aaatcgacaa attgacgaat tacttaaccg
      841 attttgacca acctctttcc ttgaatcttg gcggagggtt tgggattcgt tatacacaaa
      901 atgatcagcc gatcccagtc gaagaggcac taaaaaaact gattgaatat acggaagaag
      961 ctttggaaaa acgaaaactg accattcgtc aattactgat cgaaccgggt agaagtattg
     1021 ttggagaagc tggtacaaca ctatacacga ttggtttcat caaaaagaca ccacataaaa
     1081 aatattactt tgtagatggc gggatgacag ataatatccg tccagctctg tatcaggcag
     1141 aatacgattg tgatttagca gcaaaactgt cggaagaaaa aacagagaaa gtgacgattg
     1201 caggaaaaat gtgtgaatca ggcgatgtgg taattaaaga agcttatttg ccgaaagcag
     1261 aatcgggtga tcttttagct gtttattcca cgggagctta tggatactcg atgagcagca
     1321 actacaatcg agcgacacga ccagcagttg tttttgtcaa taatggccaa tcaagactga
     1381 ttgttcgcag acaagcattt tctgatctac taagggggga agtttcctat gatccttcat
     1441 aaatataatg gctgcggaaa tgattttatc ttattagatt acgcagaaga cacagattat
     1501 agtcaattag ccgcttcttt atgcacaaaa gaaaaatatg atacagacgg attgatcgcc
     1561 gtaaaaatcg atccgttgga aatgatctat tacaacaaag atggcagccg cgcgccgatg
     1621 tgcggaaatg gcattcgctg ttttgctcga tacgtaaatg atcagaacat gatgaatgaa
     1681 caggaattca acgtagagac attagcaggc acaagaaaaa ttacggtgtt ggatccctca
     1741 cctttttatt gcagcgtaga tatgggagtg ccagatgatt ctccggaaaa aatcaaagct
     1801 aatcaaactt atccaataaa aaaccaggaa atagagatca atggtgaaaa agtaacgatc
     1861 actagtttat ttttaggaac gattcatacc gttgtttttg ttgaaaatgc gcgtgaggaa
     1921 gtctggagaa aaagaggtga actgatctgt catcatcctt tgttcaaaga aaaaaccaat
     1981 gtcaactttg tagaagtagt caatgaacaa gaattgatcg tccgtaccta tgaaagagga
     2041 gtaggttgga cacttgcttg cggtacaggt tgctgtgcct cttatgtggt tgctaaggaa
     2101 actgggaaaa tttcagcaga tcaggtagtt gttcacttgg aacaaggaga cttgatgatc
     2161 tctggtgagg atgcgataca aatggctggc ccagcagttc atgaatggac aagaatattg
     2221 gaggaatgaa gatgataaaa ggttcgatcg tggcgctgat cacgccgatg aatgaagagg
     2281 gatcagtaga ttatgctggt ttggaaaaat tgatccaatt ccatttagat gaacaaacag
     2341 atggattatt agttttaggt acaacaggag aatcttctac gctcacacaa tctgaggagg
     2401 agcagatcct tcaactgaca gtaaagaaag tggctggccg tgtgccggtg attgcaggag
     2461 ctggaacgaa taatacgaaa gaaacgattg aaaaagcgaa gcattttgct tctttggggg
     2521 cagacgcact tttggtcatc accccttact acaacaaaac aagtgatgca ggactagctg
     2581 ctcattttac agcaattgca gaagcctcac cgattccgct tattttgtat aatgttcctt
     2641 ctagaacagg catgtcgatt ccaatccatg tattagtaaa tttagcagaa catcctaata
     2701 tcattggttt gaaagaagcc tcaggagata tggcttatgt gatggatgcc gctcggttga
     2761 ttggagaaga attcttcctt tacagtggaa atgatgattt gatcctacca gtaatgagtg
     2821 taggaggaag cggtgtaata agtgtatggg cgaatatcca acctaaaata gtccatgaat
     2881 tagtaaaaga tacgcaggac ggaagatggc agcaagcaaa agaaaagcaa ttaaatgcac
     2941 ttgaactgat ccatgccttg ttcagtgaga ccaatcctat tcctgtcaaa gcagccatgt
     3001 cattattaga cttaccttcc ggacctctac gcctgccttt agtttctctt tcaaaagaga
     3061 aaaagaaaca attagctcag cttttgttaa aagaaaggac ggttgaaaaa tgaaaatcgg
     3121 actgattggc agtggacaga tgggaaatag agtcgaagaa gttgcacaag aaagaggaga
     3181 tacggtcctt cttttacgga cggcacctaa agaagcaact acgcttgctt ttacggaact
     3241 gccagaactg cttattgatt tttctcatcc agataattta gagaaaatcc tttcgtatag
     3301 cttatcatat caacttccgt tagtcattgg cacgacaggt tatacacaat cacagttcca
     3361 agaaataaaa gaacatgcca aacatgtacc tgtgatgtat agtgctaatt tttcgttcgg
     3421 catcatggtg atgaatcaac taatcaagca agcagctgca atgttgcaag gctggcagat
     3481 cgaactgatt gaaaaacatc atagtcgtaa aaaagatgca ccatctggta ctgcaaatat
     3541 gctgcttcaa acaatccaag aagtgcaaaa gctgcagccc gtatacaact ggcaagggaa
     3601 aaaaagagaa gaaaaccaaa ttggtgtaca cagtattcgt gcaggttctt tacctggtga
     3661 acatgatgta ttgtttgctg ctactgatga aatatttacg atcaaacatg aatctttttc
     3721 taatcgaatc tttgctgcag gcgcagtaga agcagcggac tggttgaaag atcagtcacc
     3781 aggttattac aaattagaag atttattaat ggacaaaggg gtgtaaaaaa cgtggaagct
     3841 caagaaatca tccgctacat tcaaacggca tctaaaaaga cgcctgtgaa agtctatctg
     3901 aatgtaaccg agccaatcga atttaaagaa agtaaagttt ttggtgaagg aagcagcttc
     3961 gttgtttttg gtgactatga gacgattgct ccggttttag aagctgaatc agaaaaaatc
     4021 attgaaatcg aaatcgaaac tgctgcaaga tacagcgcag ttcctttgct agatattaaa
     4081 aaaattaatg ctcgtatcga accaggtgct attatcagag atcaagtgtc aatcggcaac
     4141 aatgccgtga tcatgatggg agcgattatc aatattggtg cagtaatcgg tgaaaacacg
     4201 atgattgata tgggtgctgt cttaggtgga cgagcgacag tgggcaaaaa ctgccatatt
     4261 ggtgcaggag ctgtcttagc aggtgtgatc gaaccagcat cggccaaacc ggttatcgta
     4321 gaagacggcg tactggtagg tgcgaatgca gtgattgttg aaggtgtcca tatcggaaaa
     4381 gatgcagtcg tggcagcagg agcagtggtt ttagaagatg tggcagcaga aactgttgtc
     4441 ggtggaatac ctgcacgtgt attaaaaata agtgatgatc agacaaaaga aaatacagca
     4501 ttgattgctg cgttacgcga attatagagg agatggagaa aaatgcgcga aatagatacc
     4561 tgtattgtgg gggcaaccgg attagtcggc caaacgatgc tcaaagtgtt agaagaatat
     4621 gattttccag taggacgatt gaaattatta gcttcaaaac gttcagccgg taaagtttgt
     4681 gtattcaaga aaaagaaaca cactgtacag gaattgacag aaacttcatt tgaaggatat
     4741 gatttagcgc tattttctgc aggtgcttct gtatccaaag agtttgcgcc aatcgcacga
     4801 agtcagggac tatttgtcat agataattcc tcagcatggc gggaagattc ggatattcca
     4861 ttaatcgttc cagaggtcaa tctggaagac tatacattga atcggctgat tgcaaatcca
     4921 aattgttcaa ccattcaagc ggtacttccg cttaaagctt tacaagaagc ctttggtatc
     4981 cagcgagtca attattcgac ctatcaagca gtttctggtt ccgggcaaaa aggcattgaa
     5041 gatcttcatc gtacgcaaaa tgacgaggaa ccagttttgt atccttataa tattagccaa
     5101 acggtgattc cggaaatcga tcagcctttg gaaaatgggt atacaaaaga agaacagaaa
     5161 atgataaatg agacccgtaa gattctgcat cagcctgctt tgccagtatc agcaacctgc
     5221 gtacgtgtgc caatcgaaaa tggacatgga gtctctgtgg cagttcaact gcaaaaagag
     5281 tttacgacgg aagaaattag agagtgtctc agccagtttc cagggattca attagaagat
     5341 gatctagcag cacataaata tccgacgagc attcttgcta gaggaacaga tatggtttat
     5401 gttgggagga tcagaaaaga cacttcttta gagaacggat tgttgttcta tacgactgcc
     5461 gataacatac gcaaaggagc cgctgcgaat gccgtacaga ttgctgcagc tttaatgcag
     5521 aaaatagcgg aggtggcaag atgagtaata cgctgaaagt agcgaaattc ggcggaacat
     5581 cgatgtctca tgaagagcaa ttcaaaaaag tccgtacaat tgttctttca gatcccaatc
     5641 gccaagtgat cgttgtttct gctttaggca aacgtacgtc agatgatgac aaagtaacag
     5701 acttacttta tttgatttat gcttaccttc agcatcatgt tgattggaca cccctttgga
     5761 aaaggatttg tgatcgtttc attcaagtaa gagatgttct tgccctgcag ttacctattg
     5821 aagatgaact agcacgcata cagaaacaac tggaagacga aaagataact gaagattatt
     5881 tagtaagtag aggtgaatat ctgactgctc aactgatggc tgagtatctt ggttaccagt
     5941 ttattgacgc aaaagacctg attttctttg actatgatgg gaaaatcgat caggaaaaaa
     6001 cagcacagga aatcacaaaa gcatttaaaa gatatccgaa aatggttgtt ccagggtttt
     6061 acggggcgaa ccctgcagga aaagaaaaat tattgggccg tgggggaagc gacattaccg
     6121 gcgcgatact ggcgaagatg ttgcaagcgg aaagttatga gaattggacg gatgtttcag
     6181 gaatcatgat ggctgatccg cgaatcatcg atcatcctaa aatcatcgag gaaatctctt
     6241 ttagagaact gagagagatg gcttatatgg gggctaacgt tcttcatgaa gcagctgttt
     6301 tcccagttca agaggctgat atccccatcc aaataaaaaa taccaatgat cctttcgctc
     6361 aagggacaaa aatatcgaat cacgaaatag aaaaggaaaa tggactgaca ggaatagctg
     6421 gacgcaagga ttttctttcc atcactttat tcaaacgtca tatgtccgat gaaatcgggt
     6481 ttatctggaa agcaatgagc atttttgcta aacatgagat atcaatcgaa catattcctt
     6541 cagggatcga caatatcgga gtgatcgtct cagcagaagc gattgccgat aagttatttc
     6601 tcatcacgaa agaattaaaa gaaacacttg gcgtggaaga aatcgtcgtg atcgaagact
     6661 tagctttgat ctctgtcgtt gggggtccgc ataaagaatt gattggatta tctgggaaag
     6721 tgttatcgat attgaacaaa ttagaaattc gcacatcgat cttatcacaa ggagcacagg
     6781 agttgaatct gatcatcggt gtaccgaata atcaatatga aacagtagtg aagggtatct
     6841 atgaagggat ggtgaatacg gatgctgaaa gaatatcgaa tggcactcca tcaaattcct
     6901 gaaatcgggt ttcatgaatt caagacaaaa aaatatttat atgaacaagt aaaaaacagc
     6961 ggggccgtta tccatgaaat cggcgaaaca ggactactcc tttattttga taatcagcaa
     7021 gaagaaacga ttgcttttcg aacagatatc gatgcactgc cagtcaaaga agaaacgaat
     7081 ctctcttttg cttctaagca tgaaggttat atgcatgcat gtggtcatga cggacatatg
     7141 gcgatgcttt tagggttgac tgactttata actaaacaca ttgaggatct tatttataat
     7201 gtagtactga tctatcagcc atcagaagaa atttccggtg gcgcacaaag cgttattcag
     7261 tcaggattgt tggataaata tgaagtacaa gcaatatttg gctttcatct atggcctgga
     7321 ctagctaagg gagaagtttt ctctcgacca ggacctttga tggcacaaag cagtgaaaca
     7381 gacattatca ttaaaggcca gtctgcacat attgcttcta gtgaaaaagg aatcgatagt
     7441 ctggaggcag ctgtacgatt tttgaagaat gtttatgatt ttgaccggca attaccggca
     7501 gagtggaatc atctaatgaa atttggccaa atccaaggtg gaacgatccg aaatgtttta
     7561 gctggagaag ttattgtatc cggaagtatc cgttcttaca gttcagatat gcaaaattat
     7621 ttgaagaggg gattagacca tttagcagaa cagtttcact ctacagctac tatttctttt
     7681 cgctataatg acggctatcc agctgtaatc aaccatagag gcttatatgc agcactgtta
     7741 gattcaaaca aactacatga attagatgct ccagttcttc aagccgaaga ttttggcata
     7801 tatacgcaac aatatccatg tgtatttttc tttttaggtg tcggtgatac ccctgcttta
     7861 catgatccga cgttcgattt tgatatggat gttttagaaa atggtgtaga atggtacaaa
     7921 acgattcttt ttacagaaaa tcttcttgta aaaatcgata gcagttcaaa agaggagcga
     7981 gtaaataatg atgaatttat ccaatcgatt tagtcctagg ctttctagaa tagaagtatc
     8041 aaaaatacga caattcgatc agcaaatatc gtcgatacca gatgtgatca aactcaccct
     8101 gggagaaccg gattttccaa ctcctgaaca tgtcaagcaa gcaggaatag cagcgattga
     8161 agaagacttt tctcactata ccggaatgag aggattagag gaactgcggg aagcggcttg
     8221 tatatttcag caacagcgtt atggattaac ttatgatccg caaacggaag ttttgacgac
     8281 agttggtgca acggaagcga ttgcctctgc gttgttatct gttttggaag aaggagacaa
     8341 ggttcttatt ccagcgcctg cttattcagg atatcagcca ttagtggaac tagctggtgc
     8401 tgaattgatc ccgatagata cttctgacac agggtttgtc tgtcagccgg aacaatttga
     8461 acgagcattt gaacaatatg gatcagctgt caaagcagtc attttaaact atcctaacaa
     8521 tcctactggg acgacattgt ctgcaaaaca attgaaagca atcgctgagg ttttaaaaaa
     8581 atatccggta tttgttataa gtgatgaagt atatgctgaa ttgacttata gtggaacgca
     8641 tatgtccata gctacttatc ttccagaaca aacaatcgtg ataagcgggt tatccaagtc
     8701 acatgcgatg actggttggc gagtgggctt tatttttgct caaaaagccc ttattgacga
     8761 actaattaaa gtccatcaat atctggtgac tgccgctaca acgatgtcgc aaaaagctgc
     8821 ggctgaagcg ctaataaatg gggtgaaaga tagcgagaat atgaaagagc aatatcaaac
     8881 acgaagagac tatctggtcc agcaattggc gcctttagga tttgaagtga cccagccaaa
     8941 cggtgcattt tacttgtttt gcaaactccc agctgccatt caaactgact cttggcagtt
     9001 ttgtgtagaa ttagcggaac aggcaaaagt tgcgtgtatc ccaggaattg catttggccc
     9061 agaaggagaa ggttatatcc ggatttctta tgctagcagc atggaaaaat tacatgaggc
     9121 ctgcaaacgg atcaaagact ttttaacaga acagtgattc atagatttta tcaaaaaaac
     9181 aatcgttaat tttgcggtat attctgcaag gagacgattg ttttttaaat ttagttcaaa
     9241 tgaacgcgtt tcttttgatt cataactaat gtaatgttaa gtttaaattg tttagaatga
     9301 ttttaaaaga atattcaagg aggtttcatc aatggaaaaa atcaaagttg tcattgtagg
     9361 tgcatctcat ggagggcatc aatcgatctt ggaactattg acacgatacg gtgaaaatgt
     9421 agatatcacg ctatttgaag caggagacta tatttccttc atgtcatgtg gtatggagct
     9481 gtatttagaa gacaaggtaa cagatgtaaa tgatgtacgt aatttcagac cagaaaactt
     9541 tccgcaatcc aatgttcata ttttaaataa tcacgaagta aaaacgatcg atgccgataa
     9601 aaaaacagta acggttacta gcgcatcaga taacgaaaca aaagaatatc cttatgacaa
     9661 attgattttg agttctggtg tcaaaccaaa caatctgcca gtaccaggag cagatctaga
     9721 aaatgtctac ttgatgcgtg gatacaattg ggcaaccaaa atcaaagaaa aattaaatga
     9781 tccggctatt aaaaaaatcg ctgtcgtagg tgcgggttac attgggatcg aagcagcaga
     9841 agcaagccga aaagctggga aagaagtcgt attgcttgat gtgatcgata gacctttagg
     9901 cacttattta gatgctgaga tgacagatat tttagaacaa cacctaaatg aaaacggtat
     9961 tgaagttttg acaaatgcaa aaatcgaagc ttttactggt aacggaaaag ttgaggcaat
    10021 caaaacaagt gaaaaagaaa tcccagcaga tctagtcatt caagctgccg gtgtgaaggc
    10081 aaatacagaa tggttaaaag ggatcgtcga cttagatgaa cgtggctgga tccaaacaaa
    10141 tgaatacttg caaacgaatc ttccagatgt gtatgcagta ggagatgcta cacttgctta
    10201 ttctatccca gcacgcaaaa aaatgccgat cgctttagcg actgttgcac gtagagaagc
    10261 acgttatgta gtaaaacatc tttttgaaga agtaccaaac aaaccgttcg gcggagtcgt
    10321 aggttcctct gcattaagtg tctttgacta tcattttgca gcaagcggat tgaatagctt
    10381 tacagcagca aaagcaggag tagatgtacg agtgtcttat tatgaagata cgttacgacc
    10441 aaaatatgta ccagaagaac aaggaaatcc aaaagtgtcc atccaattat tctcggatcc
    10501 aatgacccat caattgctag gaggagcagt attatctacc tatgatgtta caggtcaagg
    10561 aaatgtattg gcactagcga tccaaaacaa actgacttta gaagaactag cagaagctga
    10621 cttcttcttc cagccaggct ttgaccgaca atggagcatg ttgaatctag ccgctcaaca
    10681 agcgttagga gaagaaccat ttgtagaata agaaaacgaa atatttgaaa agaggttgtg
    10741 aaaaagctgc cttgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
    10801 taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttttcaaaaa
    10861 agggactgtg acacgatttg tgtaccgacc cccaaaagtt agaccaaaaa atctaacttt
    10921 tggaggtcgg tattttttat ggttaaatat acttttgaac ttaaattaaa gatcgttcat
    10981 gattatttgg atggcaaagg cgattggacc gtaaaaatcc agatcaagag attgaagcgg
    11041 aaatgcttaa aattcgggag aagcacaagg attatggctg tttacggatg accaatgaac
    11101 taagaaaccg cggtttctct atcaataaaa agaaagttca gcgcttgatc aagaagctag
    11161 gaataaaagt aacggcatac acgcgtaaat cacgtcgcta caattcttat cgaggaaaag
    11221 ttggagcagt agccaaaaat cggatccatc gtcgtttcta caccagtatc tgccatcaga
    11281 aaatcacgac ggatacaact gaatttaaat accatgaagt ggatgccaaa ggcattatcc
    11341 gtcaaaagaa attgtatcta gatcctttta tggatatgtt caatagtgag attttatcct
    11401 atcgtatctc tgaaaagccc aatgcattag cggtaatgga aggtttggag gaagctatcc
    11461 agaccactaa cgattgtccc tatcgtcgta cgtttcactc agatcaaggt tgggcgtatc
    11521 aaatgaaagc ttatagaaac aaattgaagg agtacaaaat ctttcaaagc atgtctcgga
    11581 aagggaactg cttagataat tcacttatgg agaatttttt tggcttatta aaacaagaga
    11641 tttttcacgg gaaagtttat aactgtttcg aggaattaaa atcagccatc gatagctata
    11701 tctattacta caataatgaa cggataaaac agaaattaaa ctggcaaagt ctcgtccaat
    11761 ttcgtaaaac aaccgtaacg gttgtgtaaa aatatcattt tagagcataa aaaaaaggag
    11821 tggatttctc cactccaaaa aagtctaact tttgggggtc actacagtgt cacagtcctt
    11881 tttttagtaa aaggatacat ttcttccttg tatattccat gcgtgttcgt gagaaagcat
    11941 atataattta attaacagat agttaggggg agaaaaaatg attttttcaa ttacaatgaa
    12001 actggtgttc gggttgatcg gtttattatt agtggtacgt ttgcttggga agaaatccat
    12061 gtcagaaatc acaccgtttg atttagtcta tacgcttgtt ttaggtggga tcttagaaga
    12121 atcaacctat gatgacaatg tccatgtagg gcatgtccta tttgccattg cgctttgggc
    12181 agtaatgatt tatggtatcg aacgtatcgt ccaaaaaaat gaaaaggtca atcgttgggt
    12241 caaaggcgag ccatctgttc taatcaaaga cggcgtcatc aacatgacag agttgacaaa
    12301 taaccatatc gagatggagc agttgcgagc aatcttaaga cagcaagaat gcttttctct
    12361 tgaaaatgcc aaacatgtta tcctagagaa tgccggtcaa atgagtgtat tgaaaaaatc
    12421 agatgaagac aaagctttat cgatcctgct tgtagatgaa gggcaaattc agcataaagt
    12481 gcttcagtcc aatcaattga ctgaagcatg gcttatggag aacttgaaga aagaaggcta
    12541 tgcagatgta aaacagctga tttatgttga atggtcagaa gaaaaagggc tctacatact
    12601 aaataaagaa gacgtcatgc atgaatctta ccgaatagat ggatagatag acagaggttg
    12661 tgaaatcagc gccttggctt gttactcaaa actaagcgac tttttcacaa cctctgatat
    12721 ctaatatacg ttgttccaga agttgcttct tcgaaaaata agccaaaatt ccacaaaaat
    12781 ttaagaagca attttcgaaa aatcctttct tattttttga agttgatcac ttctgtcaca
    12841 acctctcatg tcacaaacca tttttttgtt tcctttaact caatataccg tacgtcttcc
    12901 cacaatatcg gctgatggtc aatcataaag taatcttcat aggcttcacc aatgaagtgc
    12961 ccttcgacct catccaagag tcttccaaat tcatctttta aattgagctg aatgattact
    13021 gctttacgag ataaaaaggc ttctgtcagt atactatcga tttcttgcaa tgtcatttgt
    13081 tgtttaggaa gaggctgttt gcgtgcttct tcttcatttt ttttgatacc gttgactagt
    13141 tcttccatcg caaaagcagt cccccatttt aatccaaatg gccgatctcg atactcgtta
    13201 tagtcaatga atttttttgc tgtccgtctc atcttttcca ccttctaatc cactcatacc
    13261 acctgcatgc ccgccaacga gagaactacg tgcgatggcg cgtcctcctt gcaagagact
    13321 atgcgcatgg atgattgatt gaaagccgta tttttttcta atcgtatcga caacaaaatc
    13381 aactttcgct tcatttatct gcttttcagg atcttcaaaa aggttaagct gtaaacctgt
    13441 cgcgtatacg aggtcggaac agttcacgcc tacacgccga atatcctggt agtggtaatg
    13501 ctggtggaaa atggtcaata aagcatctgc tagatctctg ctagcatttg tcgcttcgat
    13561 tttcatttgc tggtgaaacc cggtcttgcc atttggatca agatagccta aagagtatcc
    13621 tacccataag ctgactactt gtgtttttgc tgcagatcgt ctcaagcgag tgccgacttg
    13681 gtcggccatt tctttgataa cgatctcgat ttcttctcgt cttgtataat ctctatctag
    13741 gatttgacta tttccgatac tttttgattt tggtgtatat tcttgtccca aaaaacttcg
    13801 atcgatgccc caagcatggg cataaagctg agtaccgatg acccctagct tgcttttcaa
    13861 gtgataataa tccgcgtgag ctaaatcata aacagaatgt atacccattt gtttcagacg
    13921 ttttgctgtt cgtttgccga ttccccaaaa gtcagtgata tctgagattt tccataactt
    13981 ttctggtaca tcttcataac gccattcagc aaacatttgc tttccgtctt ttgagccatt
    14041 gtctaaggct agtttagcaa gtagcgggtt atcgccaatt cctatagtcg tgtagattcc
    14101 ggtctgctca aacacatctt tatggatcag tttagccatg tcgtacgcat ttgttttacc
    14161 aaagagtgcc atgctatttg ttacatcaag gaagctttca tcgacggaat agacatgatg
    14221 gttcgcttcg tctgcatatt ttttatagat agaattgatt tctctgttct tttccatata
    14281 ataggccatc cttggggcaa caatatataa atcacttgga taggggaagg gaagatctct
    14341 ggcacggctg atattggtga tgccgtaagc tttttttgct gcaggactgg aagccaagat
    14401 taatccgcta ccccgtgttt ctgtcgaatc ggacggatag gacatgacta ctaatttagt
    14461 tgtaagggga tcaagatcct tttcaacgca ctctacggag gcgtaaaaag atttgcagtc
    14521 aatacataaa atatctcttg aaggttcttt ttcataaaca aataccggat tttgattcgc
    14581 ccacataaca atcacctctt caacacaatt agaacacacg ttctctttcc gtgtcaataa
    14641 aaaacagcaa ttttcaaata cgagcaaaag gctcacattt ggaaattgct gagaggttat
    14701 ttttatctat ttgtttttag ctgagatcgt tgattttttt atcaaacatg aacagcttct
    14761 ggatcgatct ttgttgtccc atttccaatg tgtgcaacat aaaattccgg agcatagccg
    14821 acaactttca tgtattcatc atagacattg tttttgaagg caggtatatt ttcttctttg
    14881 acaagagcaa tcgcacagcc accaaatcca gctcctgtca ttcgtgcacc taagacacct
    14941 tcttgttttt gagcagcatc tactagtgta tctaattcga tcccagttac ttcataatca
    15001 tagcgaagag agtggtgaga atcatttaac agttgaccaa atgcttctaa gttcccagct
    15061 tctaattcag cttttgcctt caaagtccgc tgattttcag aaacggcatg gcgagctctg
    15121 cgtatcaatg tatcatcgcc gatttggtca atagttgatt caaattcttc ttcatctaag
    15181 tctcctaaag aagagatagg caaagttttt tgcaaacgtg ccaaggcttc ttcgcattcg
    15241 ctacggcgtt cattgtattt tgaatctgcc aattcccgcc gcttattcgt attcatgatg
    15301 acgattgcat aaccatctag tttcactgga accatttcgt atttcagcgt atttgtatct
    15361 aataagattg catgatctag ctcaccgaag ccgatggcaa actgatccat gatacctgaa
    15421 ttgacaccga tgaattcatt ttctactttt ttacctgttt ggactagttc caagcgatct
    15481 gtttttaaat caaacagatc ttctaataca acacctacta ataattctaa tgaagcgcta
    15541 ctggaaagtc ctgcaccatt agggattgtt ccttccacta acaactcaaa cccatgatcg
    15601 attgagtgtc cagcttcttt taatttaagg accattcctt ttacgtaatt tgcccagttt
    15661 gcttttttat catattctag ctcatctaaa gtaaaactaa tgacaccgat atcgtcaaag
    15721 tttgtggaat aaactaatat tttattatcg tctcgaggtg ctgctacacc ttgcgttcca
    15781 taagtgatgg aagcggggaa gacatggcct ccattgtaat ctgtgtgttc accaatcaaa
    15841 ttgatgcgtc ctggtgagaa gtattgtgta gtttttttag gtccgaatgt ttcggcaaat
    15901 tttgtgttta agcttctttt gatttccatg atgagttatt cctttcttgt tttgattttg
    15961 aattctgtca ctgtttcaaa tactgatccg gcttttaaat gaatcgaacc aaatgctgag
    16021 aagcgctctg cacctggagc tgtttgagtc tcaaatgtga tgccaccgtg atgtgccagt
    16081 tttcggttgc gcatttctgg tgtttctgtg ccgaaatttg cagtgaaaat aaccacacta
    16141 gaagcatctg tagcaatacc tacttggatt cttttatcgg ggctagtcaa gcgagcggct
    16201 tctttaccca agccggtttc ttttaagaaa aatggatgat cgattccgtc aacaagattt
    16261 ttttgatcca aatcactagc aaaaacggac gctaattttt taggtatttg aaaatcgaaa
    16321 gcacttcctg cagcattttc ttttacccct ataggaatac tatctgttcg caaaggagca
    16381 taagcttcgc tattgagcca aagctcatgt tgatcgattg actgagaagc atctcccgtc
    16441 aagttgaagt aaacatgatt tgtcggattg aataaagtat cttggtcact ggtgccgcga
    16501 gaggtcactc gccagatatt atcttttgtc aaagtatagc ggatttccac atccattgta
    16561 cctggaaaac catgttctcc gtcgggactt gtagtagtaa aaatgacaga agcttcattt
    16621 tcgccattca aaatgacata agaccatttt tttaattcaa atccaggagc accaccatgc
    16681 agattatgtc cagtttttgg atctgtggct aattggtaag tttttccatt gagagaaaag
    16741 cggccatttt cgattctccc tgcggtgcga ccgatagaag caccgatata cggatctttt
    16801 tcgatatatt cttcagcggt gtcaaatccc aaaactaatt ctcgatcatt tgattttaaa
    16861 ctgacgattc tagcacctaa atcagagata gaaagtgcta gcttgttttt gttctccaag
    16921 gttatcaagt gataaccttg gccaaaatct ttttctgtaa tcttcatttt caagatcctt
    16981 tcttaatggg aaggaactac tgcatcgtga agttcatctt ccattgtatc agctggagcc
    17041 aatttgtttt ccaattcctc aacgatttgt gcatgttttt tctctgtcaa tgtaatcttc
    17101 caagcaaaaa taatagcgga gaaaatcatc aaagcagcag gagcatagaa tgcataagct
    17161 ttgaaagtcg ttacgccatg agcagtgata tcagcagcag tagcattacc agtcatccca
    17221 gaagcgatag cgacaaaccc tacgatcccg ttagacaaag caccagcgat tttatctagt
    17281 aaagggcgaa ttgatagagt aacagcttcg ttacggatac catttttcca ttgaccatat
    17341 tcaacagagt ctgtgatcgt catcaaagcg gataagaaaa tcaattgttg agggaagtag
    17401 aacaatacta gcccgatgat aacgattggc aaacttgaac ctgcgatcgt aaagaaaaag
    17461 taagaagaaa tcatcattgc gatccctcca aggtaaacgt agcgacgaga gatgacgcgt
    17521 gtaagcattg ggaatagtgg aacagctact aaaccaagta aggcagcaac tagaccaacg
    17581 atccaaaatt cagttggttg gccaagtaca tatttgaaga agtagaataa tacggcagtt
    17641 gttgctacat tggcaatcgc ataagcgata taagcaaggg caagccacat caactgatca
    17701 ttttgagcaa ctgctttgaa cacatctttg atcccagctt tttcattttg ctgacggatc
    17761 gcagtatcga cttcttttgt gcccaaggca gtgataatcg cagtaccgcc tgaaataaca
    17821 gcagcaatca caccgaacca gaaccagcct gaagcgcctt caatatgttt atgagtaaca
    17881 agataggtaa agaaagtaac gatagggaca acgactagag tagtaccatt tgcacctaga
    17941 gaagagccga aacgagcaaa cgtagcaaat ttttcacgtt cctttgtttc cgtactcaaa
    18001 gctggaatca ttgaccaaaa agcgatatcc ttaaatgaat aaaaactatc caataagatg
    18061 aaaacgattg caaataatat gataaataaa gtcgtattat gagtagctaa gccaaagaaa
    18121 ttggtaaaga ttgctaccaa gagtacggaa ctaacaacgc cgccgataac taaccatggt
    18181 ttgaatttac cgtattttgt acgagtatta tcgatgatgc caccaatcaa aggatcaaaa
    18241 ataatttcaa ctaagcggat acctacaact aagcttgtaa ctgtagcaat catttttgca
    18301 ttcgttgcct tatctgttcc tgcgaacatc tggcttgtca cgaataccat aaaataagtg
    18361 cttaatgtaa catagaatgt gtcgtgtcca aaagcgccta aagcgtagga cacacgttgg
    18421 atcattttgc ttttcataat ctgtattcct ctcctgttca atatataata ctaagtaaac
    18481 cggttacaac gaataacaaa gatcttttaa tgacttaaac ttaacaagaa atcgcttcct
    18541 ttttaatata tttctgtgac ttttatttaa ctttttgttg cattatacta aaggagtaag
    18601 gagggataga cgtggagcaa tcgatttttt taaaaaagga acatcaacaa gtagaaaaag
    18661 aattgtattt tgatttttgt ggtttctcta aaacgatgcc gtttcattct tttggtcctg
    18721 ctattcgcaa tgattacatc ctgcatgtcg tgatggaagg caaaggatct tatcatgtga
    18781 aaaatcaaca attccaattg aaaaaaggtg atctgttctt gattcgtcca ggagactcga
    18841 cgttttactt atcagatgga gaagatccgt ggatgtattg ctggctttca tttggtggga
    18901 gtgctgcaga agaaatcatt cggcagtcgc ttttcagaga agaccaatac acgatggtct
    18961 cttctgacat acaaaaatat atcgacatta ttttagaatg catgaaagat acggaagaaa
    19021 ccttggaaaa tgaattacag ttaacagcat taacctaccg ttttttatca gtccttttaa
    19081 aagatggcgg aaaggtcagc ggtggtgtac aaaaggttca ttcctcttta gctgttgaaa
    19141 cagtcaaata catcgaggca cattatgctg aaaaattgtc tgttgaggaa atcgcccagc
    19201 atttagcggt caatcggagc catttgtcgc gagtattcaa aaatcatatg ggtaccagta
    19261 tcaaggaata tttgatcggg gtacggatca atcgaggggc gtttcttctt tctctgacgg
    19321 atgaatccgt ggaaagtatt gcctatcaag tagggttcaa tagtctagtc gtatttagcc
    19381 gaatgttcaa aaagagtacc ggagaaacgg ctacgaacta tcgtaaacgt atgaaaaacg
    19441 aaaattttaa gaatattgct ttggatacct tgaaaaaaca gttggaaaaa caaaccgcag
    19501 tttcttggac aacctgatta gtctggttaa aatgacaagc atcggcaaaa gaaacttgtg
    19561 gcggttatgc tacactgtaa agcgaagact aaaaaggagg aaaagggatg cgtacactta
    19621 tcatcattag ccatccaacg atcgaagaat ctttaaatca gcaatttttc aaagaagctt
    19681 ctgctggttt accagctact tggcatcatt tggaatcaag ttatccagac ggaaaaatag
    19741 atatagagac tgaactacag ctcttggccc agcatgatcg gatcattttc cagtttccgt
    19801 tttactggta tagttctcct gctcatttaa aaatttggca agataatgtc ttggaacgag
    19861 cagcacatgt attaaaaggg aaagaactag gaatcgtatt gacaacaggt gtggcggaaa
    19921 aagaatacca agctggagga aaagaagaat atacgatttc cgaatttttg aggccgtatc
    19981 agcgaatcgc aaataaattc catatgacct atcttccgcc attcgtttta gcacaattta
    20041 tgtatttatc ccaggaaaaa cgttgggaga agctaattgc ttatcagcag tatctctcat
    20101 tggaaggcaa accatcgctg actcagcgga ttgactggtt tattcaaaga gtacaggaaa
    20161 atcagaaaat gcaggaagaa gaatctgaaa aacaagcata tatcgtagaa gcactgactg
    20221 aggcaaaaga gcagatcgaa gacttgacct ttacactgca agaaatgaaa ggaacttcat
    20281 tatgacaaat gattggctga tccaacaatt agatgaatta agcgaaaaat cagaatatca
    20341 agaccgtgcg ctcttatatg aaataaagaa actgatggct gagatagacg aacgaacaat
    20401 gcagctgcaa ggagaaatcg acggacaatc ttggaatcat ctcaactggt gaaattttct
    20461 tataaataac aaagataaaa atgtgcacag cgacaattat ttgctacaat aagaatatga
    20521 agcgtttgat aacgctatta tacataaaaa ttggtggtga aaaaaatgta tgatgaaaat
    20581 gagtggggag aaaatgttcg gatgacccga agaagacagc aaaaacaggc agctcgtaag
    20641 cggatgatca tgattggggc agcagttgcc gtgtttttga tcatagcagg aggagttgcg
    20701 ggtctatatt ttcatggaaa aaattcgtca gaagcgaaaa gtacgaccat aacggaaaca
    20761 acgacaaaga aaacagagac gaaacaaaca agtgagtcat caactaaaac aaaagagaca
    20821 caaacttctg aagaaaaaac agcaaatggt ttgccagagc aacttcaagc aatcgtaaat
    20881 gattattcga aaaagatcga agaaaagacg cctgcattga ttgaagaata ccagacagaa
    20941 attcagggaa atcaagaggg aatagctggt ttatcagcag ttgctaacca aaaagcacgt
    21001 gagcttcaag caatctccga cgaagggatc agaaaattac gtgcagcgta tcaggcagca
    21061 gaaaataaag acggtgtaga cttagatact ttgatcaatc aattatcggc taattatacg
    21121 aatcatgtcg ctaagatttc cgatatttat ttgcagacta gcgcctctct tcaagcagag
    21181 tcaacaagca cacaagacac aacatcttct gattctgaga cagcagagtc ggtagaagct
    21241 tctgaaatgc aagctcgaac aacacaagac acaacagatt cctctgaaac taataccaac
    21301 agcgcgactt cacaggctgc gacaaccgta gttttggaag gagaaggtcc aaatcaaata
    21361 gccgaacgaa ctggcgtacc agtagaaacg atcttgtcgc tgaatgggat gagcatggag
    21421 aattatttcc tgactcccgg tgagacgcta aaattgaatt gataaaagca ataaaaaaga
    21481 acgaagcttc cactaaggtg aagcttcgtt ctttttttat tttccggttc ttttttcgcg
    21541 tacaaagtaa tggatcgtca tgtaaagcaa gaaaacgagt ccaataatca atgagattct
    21601 cgtttcagga ttgataaaca tgaaaatcaa tacgatagct aaaaaagcaa gtgtcacata
    21661 attcgtataa ggagatagtg gcattttaaa aggatgatca tgcattttat ctgcgtgctt
    21721 ctttttgaag cgaatctcgc tgatcaaaat cactacccaa ggaaccatac caggcaaaat
    21781 acttgagctg tatacgaaaa cgaaaatatt ttctgactgg ttgatgaaca ttggtagaag
    21841 ataatttaag atcagaccaa ttaagattcc acctgagatt gctacgacag gataaaaagg
    21901 cgcaccgtga cgagatagtt tcagaaattt tttagacatc tttccattta tggcaagtgt
    21961 atagaccata cgacttgcgc tgaaaatccc tgaattgcaa cctgataatg ccgctgtaat
    22021 cacgacaaaa ttaatcagtc ctgcagcaaa tgtaatgccg actttagaaa aagtttgtac
    22081 aaaaggtgaa ccaatctcac tcaattgatt ccacggatag atactcacaa tgataaaaat
    22141 agcaccaata taaaaaatca agattcgacc gatagtggac tggacagctt caatgatcgt
    22201 ctttttagga ttctctgctt caccagcagt cattccgatc agttcgattc cttgatagga
    22261 agcaacaacg atcgataatg caaagaaaaa tcctttgacg cctccagtga agaatccacc
    22321 atttttccaa agatttgaaa tccctactgc ctgaccatga ttaccaaatc caaataagat
    22381 taccccaaga cctgcaatga tcatcaaaat gatcgttatg actttgatga tcgaaaacca
    22441 aaattctaat tcgccaaacg ccttgacaga aataagattt gccagcataa gaaacaagac
    22501 gacaatgatt ccgggaataa ttgccggcaa atcaggccac caataattca tataagatcc
    22561 tacagcaatc acttcactta ttcctacgac aatatattga aaaatattac tccaagcggt
    22621 caagtagcca gccacaggat ggatgtactc gctcgcgtaa tttgcaaaag aaccagtcga
    22681 aggatcaacg taaagcattt cgcctaaagc acgcataatc aaatacaaaa agaatcctgc
    22741 aatcgcatag gctcccatca ctgagggccc tgtccaacgt atggttgagg aggcacccat
    22801 aaacagaccg acaccgatcg ttccacctag tgcgatcatt tggatatgac gggcactaag
    22861 acttcgagtt aacttttttt cttccataca aaaactcctt tcgttgttga gtcaacaaga
    22921 aaaaataatc aaacgtttca acacatgttt ttcattatta ggttagcctt attgaactct
    22981 tgatgtgatt ctacagaaaa ttttcataaa atgcgaggaa aattcgaaaa aaactttttc
    23041 atcattctac ttgaaaatgc tttccatttt agttaaacta tatttaaata aaaagttagg
    23101 taaacctaaa aaggagtgta agatttggaa cgtacggcca ttgtaattca agacctttca
    23161 gccgcttatc aagggaaaac tgttttgaaa actatcaact tgacgataaa tccacaaaaa
    23221 attaccggta ttatcggacc aaacggtgca ggaaaatcaa catttatgaa atctttgcta
    23281 gagttgatac cagctactac tggtaaagtc accttttctg gacagccggt aaactcagtc
    23341 cgtaaaaaaa tagcctacgt ggaacagcga agtgaattgg atctttcctt tccaattgat
    23401 gtattaggcg ttgtactttt aggaacatat ccatctttac gaattggaca aagacctggg
    23461 aaaaaagaaa aagaacgtgc aagacaagct ttgaaaaaag tagggatgga agaatatgcc
    23521 aaaagacaga tcagcgaact atcgggtgga cagcttcaga gagtttttat tgcaagagct
    23581 ctagcccaag gagcagaatg gatcttttta gatgaaccat tcgtagggat tgatgcgtta
    23641 agtgaacgaa agatctttga catcttgcag gaattgaaga attcaggaaa aacgattttg
    23701 atcgtccatc atgatcttca taaagtagaa gaatatttcg atgaggttat tcttttaaat
    23761 aaacagctga tcgcttccgg tccagtacaa gaagtcttta catcgaagaa ccttcaattg
    23821 gcctatggtg agatcatcag acatttagta aaaggaggag agaaaaaatg atccaatcct
    23881 ttattgatgg attaatgaat tatcaattcc tgcaatacgc tttggtcact tccatgcttg
    23941 taggacttgc ttctggagtc atcggttctt ttattatttt acgagggatg tctttgatgg
    24001 gagacgctat ctctcacgca gttcttccag gtgtagccat ttcttatatg tttggtttta
    24061 gttacatctt tggagccact gcttttggga tgctaacagc agcagtgatt ggtttcgtga
    24121 ctcagcggag ccgattgaaa aatgatacag cgattggaat tgtctttagc tccttttttg
    24181 cattagggat tatcttgatt tcatatgccc agagtgcgac agatttgtat catattttat
    24241 ttgggaatgt cttagctgtt agggaaagcg atttgttgct gacagcactc gttagtggaa
    24301 tcgtcttgat atttgtcttt ttcttttata aagaattgaa gatcacatca ttcgatccga
    24361 caatggcaaa agcttatggg ctgaacacat cgctgatcca ttatcttttg atgttctttt
    24421 tgacattagt ggctgtagtc agtttacaga cagtaggaac aatcttggtg attgccatgt
    24481 tgatcacacc agccgccacg gcttacttgc taacgaacca tttactgaaa atgatcatta
    24541 cagctgcagg aatcggtatg ctaagtgcag ttgtcggtgt gtttttcagt tatagttaca
    24601 attggccatc aggagctacg atcgtgttag catgtaccgc attttttatc cttgcttttt
    24661 tattttctcc aacaaaagga attttatttc agaaaggaag cagatagatg aaaacaaaaa
    24721 aatcactctt tttaatcttg gcagttagtt tcttggtatt agccggttgc ggaaagcagg
    24781 cttctgacca agcggatgaa ggaagtaaag aaaagttgtc agtcgtggct accaattcga
    24841 tcttggcgga catggcaaaa gaggtcggaa cagatcaaat agatatccac agtatcgtac
    24901 cagtcggaac agatccgcat gaatatgaag tactaccaga agacatcaaa aaggcaagtg
    24961 atgcagatgt tattttatac aacggtttga atcttgaaac aggtaacagc tggttcgata
    25021 acttgatgga aacggctaaa aaagaagaag gaaaagatta ttttgcagtt agcaaaaatg
    25081 tagaacctct ttatttaact agcggtgaag aacatacaaa agcagatccc catgcttggc
    25141 tagacctatc taacggaata aaatatgtgg aggaaatcgc acgtatattc tctgaaaaag
    25201 atgcagaaaa tgcgacactc tataaaaaaa atgcagaagc atatgtggaa aaactaaaag
    25261 aattagatac acaagcgaag gaaagttttg cttctatcga agagaacaaa aaattattag
    25321 taacaagtga aggggcattc aagtattttt cacgagcata tgatttgcca gcagcttata
    25381 tatgggagat caatacagaa agtcaaggta cgcctgatca aatgaaagcg atcattgatc
    25441 agataagagc aaaagaagta ccggttttat tcgtggaaac cagtgtagac tcaagaagca
    25501 tggaacgggt agcaaaagaa acgggtttga aaatctatga taaactgttc actgattcca
    25561 tagcaaaaga aggagaacaa ggagattctt actatcagat gatgaaatgg aatattgaaa
    25621 cgatccatga aggtttgtca caaacaaaag aaagctgaaa agtgcaaggt ctagcttgag
    25681 gagcagacct tgcatttttt ataagagata tgaaaaaaaa ttatgctgtt tttcgtcgta
    25741 cagatacctt ttttgaagaa attccgttat agttagagta aggaaggaag gtgtggctat
    25801 gaattttaca cgtaaagata aatatacgat taaagaagaa gtctttcgat tgatcagcca
    25861 gcaggaaaat accgacaagg atcaagacct tcaatttctt gaagcagaat tagctaaatt
    25921 attcatagca aatcaaaatg agacaacaga accagatgaa gagctgtaca taggcaaatg
    25981 gttgtttgat gaatttcagc ctttagatga aaataccggt atcggttatt ttgtgttcct
    26041 ttctccagtc ggcttcacac ctggcgtatt agttaaacaa aacgacgaaa taaccgtcaa
    26101 agcatggcct gcattatttt cagtcatcaa agcagaagga gtaatcaaaa agatccagtc
    26161 ggcctattat aaaagtcaat ttattgccaa cagcaaatta atgaatatgt tttataatcg
    26221 tctagtagaa attgctaggc ctgggaaaat cccgcaaaca ttcacgtaca cacagcaaaa
    26281 aaatctctat gaagaagtat tgtattcccg cgatctgtct gagttttcgc catgggaaga
    26341 ggtaaggctc catgaactgt taagtacaaa accagtttca ttgaattttt ttaatgcatt
    26401 agataacaaa agaaaattaa tttcaacaaa ttattgggaa gtatttgctg caagaaaaaa
    26461 atggatggaa gaaaagaaga ttgccgatca gcagatccag tttgtccagc tgaaaaaaga
    26521 aaaactatat tatgggttat acagccgtta acagcaagcg aggaagacca tgacattaaa
    26581 agaagaaatc atacaagaaa gcaaacgatt aggaattgac aaaatcgggt tcaccacggc
    26641 ggaacctttt gattcattaa aagaatcgtt ggaagaacaa aaagcagctg gccatacttc
    26701 tggctttgaa catccaaata tcgatgagcg tctttatcca gaaatgacat ttgatcagcc
    26761 gaaatcgatc atttccattg cacttgcgta tcctactagg atccatgaag aagtgcctag
    26821 ggatgaaaaa cgcgggcagt ttgctcgtgc ttcttggggg atcgattacc atgatatact
    26881 cagagaccgt ttgaaccgat tgatcgaatt catccaaaca agagcagaaa aatggcaaaa
    26941 agaagaggaa tggcgtctag ctccgcaagt agatactgga gaactgatcg atgtcgctgt
    27001 agcacagcga gctggtttag gatttattgg acgaaacgga ttgttgatca ctgaagagtt
    27061 cggttcattt gtctatcttg gtgagatcgt tactaatatt gtatttgagc ctgataaacc
    27121 aggagaattt ggttgcggtg actgtatgcg ctgcgtgact gcttgtccga caaaagcatt
    27181 gcttggtgac ggaagaatga atgcccagcg atgtttgtct tatcaaactc agacaaaagg
    27241 gatgatgccg gaagaatatc gtaaaaaaat gcgaaatgtc atttatggat gtgatatctg
    27301 ccagttagtc tgcccttaca atcaaggaaa agattttcat tttcatgaag agatggaacc
    27361 aaaagtagac gaagtatatc caaaattaaa gccaatgctt tcgatgtcta ataaagactt
    27421 caaacagcaa tttgggcatt tagctggttc atggcgtggg aagaaacctt tgcagagaaa
    27481 cgcattgatt gctttagcta atctaggtga tcgaacggca ttgcctgaga tttcagaatg
    27541 tttaacagat gtccgccctg tcatcagagg aacagcagct tgggctattg gacgtctagg
    27601 aacaaaagaa ccggaaaaat ggcttgatgt attagatgaa atattagcta aagaaacaga
    27661 agaagtcgtt ataaacgaat tggaacatgc ccagaaatta ttgcggagaa agttaaaata
    27721 aaaaaatagt taacattatt caaagttttt gtccagtaat tgttgtaaca attactggtt
    27781 tttttgtatt ttttacattt ttttaagaga atttgataaa aatcaaaaat ttttttgctt
    27841 ttt
//
LOCUS       DADXTG010000023        27451 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.023, whole genome shotgun sequence.
ACCESSION   DADXTG010000023 DADXTG010000000
VERSION     DADXTG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.023"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            142..1209
                     /gene="rlmN"
                     /locus_tag="IWI57_000873"
     CDS             142..1209
                     /gene="rlmN"
                     /locus_tag="IWI57_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBC2449035.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1402..2505)
                     /locus_tag="IWI57_000874"
     CDS             complement(1402..2505)
                     /locus_tag="IWI57_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBC2449036.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3058..3606
                     /gene="aac(6')"
                     /locus_tag="IWI57_000875"
     CDS             3058..3606
                     /gene="aac(6')"
                     /locus_tag="IWI57_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBC2449037.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            4152..5813
                     /locus_tag="IWI57_000876"
     CDS             4152..5813
                     /locus_tag="IWI57_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2449038.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(5883..6230)
                     /locus_tag="IWI57_000877"
     CDS             complement(5883..6230)
                     /locus_tag="IWI57_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBC2449039.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            6314..7255
                     /locus_tag="IWI57_000878"
     CDS             6314..7255
                     /locus_tag="IWI57_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449040.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            7256..8299
                     /locus_tag="IWI57_000879"
     CDS             7256..8299
                     /locus_tag="IWI57_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449041.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            8305..9375
                     /locus_tag="IWI57_000880"
     CDS             8305..9375
                     /locus_tag="IWI57_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449042.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            9365..10318
                     /locus_tag="IWI57_000881"
     CDS             9365..10318
                     /locus_tag="IWI57_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2449043.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            10330..11340
                     /locus_tag="IWI57_000882"
     CDS             10330..11340
                     /locus_tag="IWI57_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2449044.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(11625..12446)
                     /locus_tag="IWI57_000883"
     CDS             complement(11625..12446)
                     /locus_tag="IWI57_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBC2449045.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(12543..12821)
                     /locus_tag="IWI57_000884"
     CDS             complement(12543..12821)
                     /locus_tag="IWI57_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449046.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            13089..15098
                     /gene="metG"
                     /locus_tag="IWI57_000885"
     CDS             13089..15098
                     /gene="metG"
                     /locus_tag="IWI57_000885"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBC2449047.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(15577..16164)
                     /locus_tag="IWI57_000886"
     CDS             complement(15577..16164)
                     /locus_tag="IWI57_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449048.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            16339..17661
                     /locus_tag="IWI57_000887"
     CDS             16339..17661
                     /locus_tag="IWI57_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2449049.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            17816..18592
                     /locus_tag="IWI57_000888"
     CDS             17816..18592
                     /locus_tag="IWI57_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBC2449050.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            18585..19154
                     /gene="rnmV"
                     /locus_tag="IWI57_000889"
     CDS             18585..19154
                     /gene="rnmV"
                     /locus_tag="IWI57_000889"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBC2449051.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            19147..20031
                     /gene="rsmA"
                     /locus_tag="IWI57_000890"
     CDS             19147..20031
                     /gene="rsmA"
                     /locus_tag="IWI57_000890"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBC2449052.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            20482..21111
                     /locus_tag="IWI57_000891"
     CDS             20482..21111
                     /locus_tag="IWI57_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449053.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(21235..22344)
                     /gene="ugpC"
                     /locus_tag="IWI57_000892"
     CDS             complement(21235..22344)
                     /gene="ugpC"
                     /locus_tag="IWI57_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBC2449054.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            22553..22975
                     /gene="mgsA"
                     /locus_tag="IWI57_000893"
     CDS             22553..22975
                     /gene="mgsA"
                     /locus_tag="IWI57_000893"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBC2449055.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            23298..23834
                     /locus_tag="IWI57_000894"
     CDS             23298..23834
                     /locus_tag="IWI57_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBC2449056.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(23874..25637)
                     /locus_tag="IWI57_000895"
     CDS             complement(23874..25637)
                     /locus_tag="IWI57_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449057.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(25640..27355)
                     /locus_tag="IWI57_000896"
     CDS             complement(25640..27355)
                     /locus_tag="IWI57_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449058.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         8858 a         4970 c         5566 g         8057 t
ORIGIN      
        1 ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa gttctattcg
       61 atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta gcgaaacttt
      121 gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac gaatgaagaa
      181 ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca agtttgggaa
      241 tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc gaaacaattg
      301 atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt cgtccaagaa
      361 gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat gattgagact
      421 gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt aggatgtaat
      481 atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct gactgctgga
      541 gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa tcttggcgaa
      601 cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta cgacaatgta
      661 atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc tcggcacatt
      721 acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa cggcttgcaa
      781 gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg
      841 atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta tttagagaaa
      901 acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga tagaccagag
      961 catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata tgtcaacttg
     1021 attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa agctgatgta
     1081 ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat
     1141 ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt
     1201 gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag ttttcttaat
     1261 taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta taaagctttt
     1321 tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga cagcctgctt
     1381 gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt
     1441 agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata
     1501 aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt
     1561 cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac
     1621 atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata
     1681 actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata
     1741 gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt
     1801 agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact
     1861 atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg
     1921 gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac
     1981 ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat
     2041 aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat
     2101 tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat
     2161 agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca
     2221 agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat
     2281 caactgttca atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc
     2341 tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc
     2401 tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc
     2461 atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt
     2521 tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg
     2581 agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat
     2641 catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat
     2701 ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta tctaaaaatt
     2761 ctaaaaatta agattattat atgaaaacag gttttatttg tcaattcata attggttttt
     2821 tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat
     2881 ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt ttattttata
     2941 agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag
     3001 aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg
     3061 ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg
     3121 agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg
     3181 aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt
     3241 gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc
     3301 cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc
     3361 agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt
     3421 caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa
     3481 catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca
     3541 aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct
     3601 caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac
     3661 gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg
     3721 aacaccgtgt accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt
     3781 gtgaaaatgg ctttctacat ttttgcataa atttggctta ataccgagaa actggctttt
     3841 gaacaccgtt tattcagata agggactgtg acaagacttt tgtcacagtt tctttttgtt
     3901 tattcttttt tatctttgta tggcaataat agacaatata acgaaaatta tttaatggct
     3961 tttggaagac catggaaaac cttgtacaaa gctatttttt ctgaaaaatt ccttgaaacc
     4021 tcacgaaaaa atcattattt tatgaaaaag acaaaaaaaa gtgttgattt tttaataatt
     4081 attcattaaa ctcttaaaca acttcagaaa atattaatta ttctgaaaat tccaataaag
     4141 gggtacgtaa aatgaaaaag aaatttacat ttggtttggt tgcggtatgc ggccttgtac
     4201 ttgctggatg ttatggcggc agttcagcca caagcagtag ttcagcaaaa agcggttcag
     4261 cagatggagg aggcgtattt aacttagtcg tgcctcaaga aatgcctaca gctgacttat
     4321 cagttgcaac agatacaatc agttttacag cattgaataa cgtttatgaa ggaatttacc
     4381 gtcttgatga agacagcaaa ccacagccgg caggagctag cgagttggca gaagtcagtg
     4441 aagacggatt aacttacaaa atcaaattac gagaagacgc aaaatggtca aatggtgatc
     4501 ctgttacagc agcggattac gtttatggct ggcaacgaac agtagaccca gctacagctt
     4561 ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga agacattaca gctggaaaga
     4621 aagataaatc agaactaggg atcaaagcag ttggtgacta tgaattagaa atcaaattga
     4681 cgaaacaaac accatatttc caatatctgc ttgctttccc atcatttttc ccacaaaaac
     4741 aatccgtagt ggaagaaaat ggggatgcct atgcatctgc aagtgataaa gctgtttata
     4801 atggaccatt cacattagaa gattttgatg gaccaggaac agatacagaa tggacttata
     4861 agaaaaatga tcattactgg gacaaagata cagtaaaact ttctgaaatc aaagtcagtg
     4921 tagtaaaaga atcttctact gcattgaact tattcaaaga tggccaagca gatgacgtga
     4981 tcttgtctgg tgagttagca cagcaaaacg caaacgatcc agcttatact tctgttaaag
     5041 aagcgcggac aagctatatc gaattcaatc aacgtgaaaa gaattcacca ttcaacaatg
     5101 tgaacctaag aaaagctatt tcttattcta tcgatcgtga agctttagta aaacaagtac
     5161 taggtgacgg atcagttgtc tctacaggat tgatcccatc agatatggta aaaagtccaa
     5221 caacgaacga agactttgca aaagaagctg gaaaattagt ttcttatgac aaagataaag
     5281 caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta gaatttgatt
     5341 tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa aactcaatcc
     5401 aagaaaactt ggatggcgta acagtcaaac caacacctgt tccattctct gtacgtcttg
     5461 accgctcaac ttcgggtgat tttgacgcag tactaggcgg atgggctgct gactatgcag
     5521 acccaagcag ctttactgat ttgtttgtca caggtaattc atacaaccgt ggtcgctgga
     5581 gcaatgcaga atatgacgaa gcagttaaag cagcttcatc aacagacgct ggcgatgaac
     5641 aagcccgttg ggatgatttc caaaaagcaa tcaagatcat ctctgatgat atgggtgtcg
     5701 ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa ggtatcgttc
     5761 accatgcggc aggcgcttca tgggattaca aatggacata cgttgaagaa taatcgttaa
     5821 taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt tctcatattc
     5881 acttatccta acaaagaaaa aagcatgaaa atagcagaaa gagcaattag tattccggca
     5941 atgatcagcc aaaagctgta cataccgtag ctggcagacg agagcgagga aaattctggt
     6001 tttttctttc ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg atcgaagaaa
     6061 cccgaagaaa agacccataa aaaaccgccg ataatcagaa acggcaatga ccataagaat
     6121 agatcattag ataattgaat gagtgtaaag gaatggttga tgaggttttt tattaaaata
     6181 accagaatca gtagaaaagc tactgcgtag gggatagatt tttttttcat ttgattgctc
     6241 ctttattttt ggttcattat ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta
     6301 gggggaaaca ttagtgaata gttatatcaa atatgtatta aaacgtgtct ttttcatgat
     6361 catcacgctt tggttaattg caacaatcac ctttttcttg atgcaattgc tgccgggcac
     6421 gccatatacg aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt
     6481 cggtttggat aaaccagtta tcgtacaata tgggatttat ctttctaatt tgcttcaagg
     6541 agattttgga atttccttcc aatttaaaaa ccagccagtc gcacatttgt tagctggaag
     6601 aatcggacca tccttgcagt tagggttaca ggcaattatc tttggaacag tcctagggac
     6661 gattttagga acgatctctg ccatgaagca aaatacatgg gcagatacat cttctacact
     6721 cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt gctgttttat tacaatatat
     6781 ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg gatggctttg tgtacacaat
     6841 cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac
     6901 ggaaatggtg gaagtactgc atagtgatta tgtggaatta gcaaaagcaa aaggattgag
     6961 ccgttgggaa atcgccttta aacatggtct tcgaaatagt ttgattccat tgatgacttt
     7021 attaggaccg ttagctgtag cattgatgac cggatcactg gtggtagaaa atatctttgc
     7081 tattcctggt atcggggaac aattcgttaa gtcaatcaca acaaatgatt atcctacgat
     7141 catggctgta acgatccttt actcatttat gctgattttt gttattttga tcgtggatct
     7201 attgtacggt ctagttgatc caagaattcg tgtttcagaa gggagccgag gataaatgga
     7261 aatgatacca aagcgttatg aaacagttgc agatatccct gcggatgaat ttcttccatt
     7321 acaaaaaaat accgaagaag aacgtgaaca gattactgca ccttctttga attttatcca
     7381 agattcatgg cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg ttttactatt
     7441 ggtcatcatt ttgatctctg tgatcacaat ctttgtttct cctcatgatc caactgccca
     7501 aaacgtggct tatatcaatt tgcctcctcg tattccggga atcaatatcg atggattgaa
     7561 tggaaaagca atggttggtg gtgaacttgt tgataaatat gctcaagcaa acgtcccttc
     7621 aaacgtaaat ttctttttag gaacagacgg tttgggacgt gatgtattaa gccgcttatt
     7681 tatgggaatg agaatctcgt tgctgatcgc atttattgct gcattactag atgtaacgat
     7741 tggtgtagct tacgggttgg tttctggttt gttaggcgga cgtgtcgata acgtgatgca
     7801 acgttttttg gaaatcttat caggaattcc aaacctcgtt gtcatgattt tgatgttggt
     7861 cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg gccatcacaa actggatacc
     7921 aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat atgttttagc
     7981 tggattgaca ttaggtgaat caagaatgaa aattgcattc aaacatattt tgccgaatat
     8041 ctctagtgtc attattattc aaatgatgtt tagtattcca tctgctattt tcttcgaagc
     8101 ctttttaagc tttatcggtt taggtcttac gcctccgtca gcttcattag gaacgatgtt
     8161 gagcgaaggg tacaagacat tcctgtatct gccttatcta ttatggattc cggcagtaac
     8221 tttatcggtt atcatgattg gctttaactt acttgctgat ggattgcgtg atgcctttga
     8281 tcctaaaatg aaagagtgaa gacgatgacg aaaattttag aagtgaaaga cttagaaatc
     8341 tcttttgata cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat
     8401 aaaggagaaa cattggcgat cgttggggaa tctggaagtg gaaaatcagt gacgaccaga
     8461 agcatcatgc gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat cctctttaaa
     8521 ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg taaagaaata
     8581 gcgatgattt tccaagatcc aatgacctct ttagatccga cgatgcccat tgggaagcaa
     8641 gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa aagagggaca aaaagctgca
     8701 ttagaattgc ttaaattagt tggaataccc gaagctgaga aacgtatcaa cagttatccg
     8761 catcagttct caggaggtca gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat
     8821 ccggaaatcc tgattgctga tgaacctaca acagcgcttg atgtgactat tcaagcacag
     8881 attttggagc tgctgaaaga aattcagaca aaaatcgata catcaattat ctttatcact
     8941 catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga
     9001 atcgttgaag ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg
     9061 ggactgcttg gttcaatgcc gacattagat agtgcgaacg atcgattgta tgctattcct
     9121 ggttctccac cggatttatt gaacccgcca aaaggagacg cattctatcc aagaaatgaa
     9181 ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt ttgaactatc aaaaacacac
     9241 aaggcagcaa cttggctact tgctccgcag gcacccaaag tgacaccgcc agaagaaatc
     9301 caacgtcgct gggctatctt taaagagaaa caaaaacata cttacggtca aggaggaatt
     9361 gctgatgcca aataaactac ttgaagttaa aggattgaaa cagtatttca atgtaggaaa
     9421 aaagaatgaa gtacatgctg taaatgacat cagctttcac atttatgaag gagaaacatt
     9481 cggtttagtt ggagaatctg gaagcggaaa atccactact ggccgtacga tcattcgctt
     9541 gaatgaacct actgatggtg agatattatt cgacggtcaa gatataacga agatcaaaga
     9601 taaaaaaggt ttgacgaaat tccggcatga tgttcagatg atctttcaag atccatacgc
     9661 ttcgttgaat cctcgtatga aagtcagaga tatcattgcc gaaggaattg atgttaatgg
     9721 actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat gatctattga gaactgttgg
     9781 attgaatcca agccacggta ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg
     9841 tattggtatt gcacgagcat tggcagtaaa accgcgtttt atcatttgtg atgaaccaat
     9901 ttctgcacta gatgtgtcga tccaagccca agtggtcaat ttgttgcaag atctccaaaa
     9961 agaacatcaa ttgacctatc tatttattgc acatgatttg tcgatggtta aacatattag
    10021 tgaccgaatc ggtgtaatgc ataacggctt gttattagag atgggaacaa gtgatgaaat
    10081 ctataatcac ggtgttcatc cttatacaga aagtttgtta tctgctattc ctctaccaga
    10141 tccagaccat gagagaaaac gcagaagaat caaatatcaa cctgaaccag atgacggaca
    10201 agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg agcaggaagt
    10261 cccatattac gcaaaaaaac taaaacgaca aaaggaatct cttctggttg ctaactagga
    10321 gggacataga tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga acaaaaacag
    10381 caattgattc gtacttttgg atgtgctcga tttacctata accatttgct gaaaaagcgg
    10441 cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc tcacacccgc tacattaaaa
    10501 aaagaatacc cattcttaaa agaagtagac agtcttgctt tagcaaatgc acagcttaat
    10561 ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg
    10621 aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat ctatctggaa
    10681 aatggctact taaagttacc aaaacaaaaa gaactaatca agataaatca gcatcggcca
    10741 gttgaaggat cgattcgctc cgctacgatt tctgctcgat ataatgaaga attttatgtc
    10801 gcactacttt gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg gatcgggatc
    10861 gcctatcatc cgaagacatt gatccaaaca tctcgacctc tagaagtaac tttaccaaaa
    10921 tttcaccaga cagaagaaaa gctgcaacat gcacagcgga aattgaatgt gaaagtccgc
    10981 tccgctcacc atagaaagat caggttagat caggcaagca attatcagaa acaaaagaga
    11041 aaagtaatgg atctttatct gaaacaaaag aaccagcgag aagattatct tgaacaatta
    11101 tctgggaaat tggtcaaaca atatgactac ttatttgtag aatcttttcc aaaagaagag
    11161 gcacatgctg atttttctat tcacgactgg cataaattaa taacaaaatt gcgttataag
    11221 tcccaatggt ataataaaaa atttcttttg atcaataccg acggagcaga agaaagcaat
    11281 tccgtaagaa agagccaagt agtagaaaaa atgggcagac attcggttat caagggataa
    11341 aaaatgaatg aaactgatag gagcgttagg gttagccttg ttaataaaaa gtcttaatga
    11401 cttttgtttc taggaatttt tcttttgaag aaaagctcac taattatgac aaaccaataa
    11461 aacaatagca gaaaaagttc gtacgagctt tttctgctat tgttaatttt ttattcagaa
    11521 agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg
    11581 cgttttttct tgaatgatta ggtctgaatc ttttgctcac aaccttattc atggtgcccc
    11641 ggcttgagct gatagatttt tgaaggacgg ccgattccta caggacgttc accgatctca
    11701 tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc
    11761 cctaagaatt tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt acctaaaact
    11821 tgtagaactt gcggctcgtg ttccatttta ttgactacac ggttgaatgc tttcaagatg
    11881 atttgactat ggtcaaaagc caaagtatct ttgccagaag actctccagt gactaaatca
    11941 agcgagatag ccacttctcc gctagataaa tggatcttgt tactatgacg ttctagtgtg
    12001 aaccagcgta cttccttggc atcatctccg gcgattagcg gttcttctcc aataaaggct
    12061 aagtagctga ctgtgacgac ccagcctcgc ggatctcgat taggcgtact gaatgtatgc
    12121 agttgctcaa tattttcttt tgaaataacc acaccagttt cttcctttgt ttctcttaag
    12181 acgctttctc cagttgactc atccttttgg acaaagccac caggcaatgc ccatgaattt
    12241 cgataaggat gcccttttct ttggatcaac aaaactttca gctgatcttc ttctttgtta
    12301 tagcacatca gtacgatatc gacagtcaaa gagggcttgt catattctgg acgttcctgt
    12361 ttttgatacc agttaagaaa ttcttcttca cttgcttcat gttcataata taatttttct
    12421 tcttctttag aagcaaaacg gttcatctga ccacctccat tgacagtaaa agtacttttt
    12481 accttattgt agcagactct gaaaaaaaac caaggattta cttgaaaaat ctaggttatc
    12541 atttaaatat gaaaaataaa tggaagtatg agattgaata aaaagaaaag cagataaaac
    12601 ggcaatactt tataccgctc ttcacgaatg gcaaagaagc cacaaataac gatatatacg
    12661 tcaatgataa aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg cccatggagg
    12721 ttaaaataac catacatacg aatataaaaa tagctgaata ttaaatgtgc taatgaaaga
    12781 aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc agtcccttac
    12841 taagatagat agtctttatt ataccacgtg tgaggggaga aataaaaagc cgttttttag
    12901 tagatatgaa acaaaacaaa aacccgcatt tattcaacct attcatcctt ttaaaggata
    12961 gattgaacgt agatagaagt cgtttatcca gtctttttca ttttgaaaaa aagctaacag
    13021 catgcttttt ttatggtata ctagcaagga aacgaattcg catcatgcga taaaggagga
    13081 agagccccgt ggcagaaaag gaaacttttt atatcactac gccaatctat tatccaagcg
    13141 gcaaacttca tatcggcaac tcgtatacta cgattgcatg tgacgcaatt gctcgttata
    13201 aacggttgat gggctttgat gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa
    13261 tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg
    13321 ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta tgataaattc attcgcacga
    13381 cagatgatta ccataaagca gctgttcaaa aaatatttga tcgtttatta gaacagggag
    13441 atatttactt aggtgaatat gaaggctggt actctgtttc tgacgaagaa ttctttacag
    13501 aaacacaact tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc
    13561 caagtggaca cgaagtagaa ttggtaaaag aagaatcata cttcttccgc atgagcaaat
    13621 acgcagaccg tctattggaa tactatgaag aacatcccga attcatccag ccagaatctc
    13681 gtaaaaacga aatgatcaac aactttatca aaccaggatt agaggatctt gcagtttcac
    13741 gaacaacttt tacttgggga gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat
    13801 ggatcgatgc attatccaat tatattactg cactaggata tggctccgaa gatgaaagtc
    13861 tattcgaaaa atactggcct gcagatgttc agatggtcgg taaagaaatc gttcgctttc
    13921 atacgatcta ttggccaatc atgctgatgg cccttgattt gcctctacct aaaaaagtat
    13981 ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg
    14041 tttatcctga aatgcttgtg gaacgttatg gtttagatgc actccgttat tatttgcttc
    14101 gtgctgttcc gtttgggtca gatggtgtct ttacgccaga agattttgtt tcccgtgtca
    14161 attatgatct ggcaaatgat ctagggaact tattgaatcg aacgattgct atgatcaaca
    14221 aatattgtga tggactcgtt cctgactatg cctcacttgt cactccattt gatagtgaac
    14281 tgtctacgac ggctgctaac gttgtcggac gttaccatga tgcaatggaa aaaatggaat
    14341 tcaatacagc attggctgaa atctgggtgc tgatttctcg tgcaaacaaa tacattgatg
    14401 aaacagaacc atgggtatta gcaaaagacg aagaaaagaa agctgaattg aatagtgtca
    14461 tgatccattt agctgaatct ttgcgtatcg cagcgatatt acttcagcca atcatgacag
    14521 aaacgccaga gaagatcttc aatcaactag gattggactc tgaaacaatg aatcttgaag
    14581 gattacactt tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct
    14641 tcccacgttt agatatggaa gaagaagttg cttttattca agaaaaaatg tctgaaggta
    14701 ctcaaacaaa cgaagataca gtcaaatggg atccagaaga aacagaactt gtttcaacga
    14761 aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga actaaaagta gcagaagtca
    14821 tcaactgtca aaaagtagaa ggggcagata agttgctgca attccgacta gatgctggag
    14881 acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat
    14941 tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg cgtggacaaa
    15001 tcagtcaagg aatgatcctt tctgcagaag caccagatgg ctctttacaa gtaatcgaag
    15061 caccaaaatc catgccaaat ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac
    15121 tagttgtgtc ataccccctt tttccgaaat ttgagaagca attttcgtaa attccttctt
    15181 attacttgaa gccaatcata taatacagaa acagagaact atccaaaata aatcctagaa
    15241 aaagaagtac gagcttcgct gatttttgtt aatttagaat aatactaata ggaatcgtaa
    15301 aaacagaaaa aaagcataat gataaagggt cgggcagatt ttaattgatt catctggtac
    15361 tcttttttat tgtccttaca ttcataaagt ttgaatgcaa tgactttatt acatctatat
    15421 ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta tgttcaatta
    15481 gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat cggctttgac
    15541 ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat ggtaaccttt
    15601 tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa
    15661 gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg tcaacatcac
    15721 gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat tatcccaatg
    15781 catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga tcccgactaa
    15841 ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag
    15901 caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc cccacaagat
    15961 catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca atattagttt
    16021 ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc aatgccaatg
    16081 ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat ctgctaattt
    16141 cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg taggtttagt
    16201 atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac tgaaagtgga
    16261 gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac caaatttcaa
    16321 agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca
    16381 ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt
    16441 actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg tggtttctta
    16501 acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga aactggtgga
    16561 gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt aggatgggcg
    16621 caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt tgcgacacaa
    16681 ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt gatcaccgct
    16741 gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg
    16801 acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt attgacccca
    16861 ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac ttttgtcgaa
    16921 ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt aggtgtcggt
    16981 gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat tattcttggt
    17041 ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa aactttgccg
    17101 attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt catttttgga
    17161 ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg tgcattgaac
    17221 ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct
    17281 ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt tcctgcggtt
    17341 ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt tttaacagac
    17401 atgttgattt ttgtgatgtg gctattcagt ttactgattt ttatcggtgt ctttatttta
    17461 agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc aatcgttccg
    17521 attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac gcaaacgaaa
    17581 ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta tcaaaaaaag
    17641 aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac gaactaggtc
    17701 ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt tgatttttgc
    17761 tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggagaga taaacatgat
    17821 atttgattct catacccatt taaatgctga acagttcaat gaagatattc cagaaacgat
    17881 tcagcgagca caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg atacgccaac
    17941 gattgaaaag tctttgcaat taagccatga ctatccaaat atatacagta ttatcggctg
    18001 gcatccgaca gaggctggca gttatacgaa agaaatcgaa aataaattac aagaacaatt
    18061 gactttacca aaggttgtag cattagggga aattggcttg gattatcatt ggatggaaga
    18121 cccgaaagaa gtccaagata aagtatttcg cagacagatt gctatcgcaa aagaaatgaa
    18181 cctaccaatc agtattcata ctagagaagc acttgaagac acgtatcaaa tcctaaaaga
    18241 agaagatgtt cgagatatcg gtggaatcat gcatagtttc agtggtgatt atgaatgggc
    18301 aaaacgattt cttgacttag ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa
    18361 agcattagac gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac
    18421 agatgcccct tatctggcac cagttccttt tagaggaaag cgaaatgaac caggctatac
    18481 tcgttataca gtagaaaaga ttgctgaact tcggaatctt ccgacagaag aagtggctgc
    18541 ccaaacatgg acaaatgcgc accgattatt tggtctaagc gaacatggat aagatgaaga
    18601 tccaagagat tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg gaagtagttg
    18661 atgcagatac gatagaaaca atagggtctg caatcaatga agagatcctg atgcaaattg
    18721 aacatgccca agagactcgt ggagtgatcg tttttaccga tcctgatttt tcaggagaaa
    18781 aaataagaaa aatcattatg gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca
    18841 cacaagctaa accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta
    18901 ttttagaagc attgaaaaag gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa
    18961 tcactagaca agacctttta gactatggtt taatagctgg ggcacaagca aaagaaaaac
    19021 gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac
    19081 gtttgaagat gtttcgtatt acgccgaaag aactcgccca agcaatgaaa atagtggagg
    19141 aacaacatga gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga gattttattg
    19201 aaacatggtt tttcatttaa aaaaagttta ggacaaaatt ttctgactga accaaatatt
    19261 ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat cgaagttgga
    19321 ccaggaatcg gtgcattgac ggaacaatta gccaagcatg caaaacaagt cgtagcattt
    19381 gagatcgatg atcgtttgat ccctgtattg gctgatacga tgcaacccta tgacaatgtg
    19441 actattgtac atcaagatat tttaaagacc gatttgtcaa cagctgttag agaaacattt
    19501 catgaggagc ttccgttaaa agtcgtagcg aatttacctt actatattac gacaccgatc
    19561 atgatgcatt ttcttgaatc agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa
    19621 gaagtagctg atcgtatctc tgctgaacca ggaacgaaag cttatggttc attatcgatt
    19681 gctgttcaat attatatgga agccagttta gcattcatcg taccaaaaac agtatttgtc
    19741 cctcagccaa atgtagattc agcgattttg aaactgacaa gaagagatat accggcagtc
    19801 gaagtgacag acgaaaaagc atttttccga ttgacaaaag cggcatttca gcaaagaaga
    19861 aaaacgcttt ggaataactt gcagcattcg tatggcaaag atgaccagac aaaagcatgg
    19921 ttagcaaaaa gcttggaaac agctggaatc gatccaaaac gtcgtgggga gactttgtca
    19981 ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg
    20041 ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa
    20101 atagttcctc atgtttttcg tttgtttggg cttattaccg cagatgcaag cttttgaaca
    20161 ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg aacaatttca
    20221 aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca agcttttgac
    20281 cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct atttttgttt
    20341 cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat ttagaaaaga
    20401 atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt atgtgtcaag
    20461 acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac gatttctgct
    20521 ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat gattccacca
    20581 gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt tacaccagga
    20641 gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac gccggttatc
    20701 atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg gtacttgcaa
    20761 aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg gcgtttccaa
    20821 gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt agtcagcatc
    20881 ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt ttatacagta
    20941 ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa tattgcttat
    21001 tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc agcaaaaaaa
    21061 gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta gacatgatct
    21121 ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg agagtttttt
    21181 gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg caatttatgg
    21241 aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga tgttgaaggc
    21301 aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg agatgaactc
    21361 agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg aaacgacaac
    21421 ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac tgtggatgtc
    21481 ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa ctaatacttt
    21541 gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa caccattttt
    21601 caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa cgaacatatt
    21661 gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc cgtctttcat
    21721 gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa catagatggt
    21781 cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta cacgtaattt
    21841 agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc gaacgattgc
    21901 ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa
    21961 gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga tgtctgcttt
    22021 atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca tatgaggata
    22081 caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca cgtcattcat
    22141 gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg cgatcattcg
    22201 caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt cacggtcttt
    22261 gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt ttttatatac
    22321 gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag ttttattaac
    22381 tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac aagaattttt
    22441 tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccataag aaataaaaac
    22501 tgatacactg gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat
    22561 cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa
    22621 aacaatattg gaaaaacatg aactttttgc taccgggacg actgggcaga ggatcacgga
    22681 agcaaccggc ttgccgatcc atcggtttaa atccggaccg ttaggcgggg atcagcaaat
    22741 tggagccatg atttcggagg atcagttaga tttggtgatt tttcttcgag acccgctggc
    22801 tgcacagccc catgaaccag atgtaacagc acttatccgt ttatctgatg tctatgagat
    22861 tccgttagct acgaatatcg gaacagctga ggtgctacta agaggactgg aagccggctt
    22921 cctagatttt cgaagaatcg tccatgaaat agacaagcat ccactttcat tttgatggat
    22981 aagcgtatgg aaaaataacg tttaatgttc gcctatatgt agctataaaa gctaaacaaa
    23041 ctgataatat gcgatataat acgaacgtat tggataatat aaacaataat attagttttt
    23101 cgtatttgca gtaaatgtga aaatatgcta tagtttctgt gggaaaagat cccaaaaata
    23161 atacagagta gaaaataaag cgttaagtgc taagaggatg ggatgttgcc tcttggacga
    23221 aggacgtaat gttcgcggtt ttattttcgc attcgctgca tgatacgatg tagcagctct
    23281 taaaggagat gctaaaaatg aagaaacacc ggttagacgc acgtatgatt gctattatgg
    23341 gcttattgat tgcattgatg gtcactttgt cccgactggt agcaatcgag accccattta
    23401 ttaaaatcag tgtcacattt attccacaag tgatcatggg cattcttttt ggtccttttt
    23461 ggtcagggat aggcgcagtt ttagctgatt tagtaggaat ggccctcttc tcgaaatctg
    23521 cattctttat tggatttaca ttgaatgcat ttattgaagg agcaatctac ggattctttt
    23581 tctatcgaaa agagatcact tggaaaaatg ccattctggc tacgcttagt gtcacgctga
    23641 tcatcaactt atttttgaca ccattatggc ttgcgttgat gtaccatgtg ccattgttca
    23701 gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat atggttaccg attcaatcaa
    23761 ttgccattta ttacgtagga cgtagtattc cttacaaaaa aattttaaga agtttagcga
    23821 tccacgctaa ataaaaaaca agcagtcttc ccatcccaga agactgcttg tttttattgt
    23881 gctgtgaatt gtgcttgata gagccgtcgg taataaccat ctttttgaag cagctcatca
    23941 tgggtgccca tttcgacaat agctccctga tccatcacta atatctggtc cgcatttttg
    24001 attgtggaca ggcgatgggc aatcacaaaa ctggtttttc cttccatcat tgctaagaag
    24061 gcttgttgga tttttttctc tgtcaatgta tcgactgagc tggtagcttc atctaagatc
    24121 aacatagggg gattactgat catcgttcga gcaatcgtca acagttggcg ttgtccatct
    24181 gatatcttta acccttgacc gccaatctta gtatctaatt tttccggcaa acgttctaca
    24241 aattcataca tataagaggc ttttagggca tcattgattt ctgcgtcact tgcttccgga
    24301 ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc
    24361 attccaaaac ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg gtcatcgact
    24421 gtaattgttc cttttgttgt gtcataaaaa cgcatcagca aattaacaag tgtggatttt
    24481 ccggcccctg tttttcctac tatagcaatg gtttgacctg gataagcagt aaaattgaaa
    24541 tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa agagactttt
    24601 cctttgatat ttttgggttc caaggcatag gcatctggca aatcaggctt ttcttctggc
    24661 tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg
    24721 attccagaaa gttcgataaa tggtttagaa aactgacttg agtagatcgt aaagctggaa
    24781 atcacaccaa cagtgattga cgaaccactc ttcaatgcca ataggccgcc aactaaacca
    24841 atcgataaat aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa
    24901 aattgtgccc gttgtccttt ttcatataga tcagcattta gcagttcaaa ttgctgttga
    24961 ctagcttctt cttgctgaaa agctttcacg attttctggt taccgattcg ttcggtcaca
    25021 aagcccgaga tatccccaat aatcctttgc tgtctatcga aatttgtttg tgaagcacga
    25081 gcaacgatcc aactcacaag gaaaataatt ggtgtagaac aaagaacgac caatgtcaga
    25141 agtggactta gacgcatcat aaaaataaac gaaagaatga tgattgccac tccagaaaat
    25201 aattgattga aggccgcaga aattgcaatg gaaatattgt ccatatcatt tgtaaatcga
    25261 ctgacgatat ttccgtggga gttttgatca taataacgaa gtggcaatcg attcagatga
    25321 gcaaatgcat cttttcttaa ttttgcaacg gataagtaag ctaaccgatt gcttaacact
    25381 tggatgagcc attgactgac tacgttgatt aaaacgatac caccgaatag aataagtaaa
    25441 tggaacagtc gattgaagcg gacctgtcct tctccaatca tttgatcgat actttgacca
    25501 atttgtaacg tcatcacaac tgttgcaatc ccattgatga ttcctagtat cacagctagc
    25561 caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat
    25621 aattttttta ctttcataac tattcttcct cctgtgatgc gacgatttcc tgatattctg
    25681 ctgattgagc aagtaattct tcatgggtac cctgagcagc aatcttacct tgatccatca
    25741 ctaagatatg atgtgcttct tgaatagagc ggacacgttg ggagataata atcactgtac
    25801 tgccaagata agtctgtagc gccttacgca aatccaagtc tgtttgataa tccaacgcac
    25861 ttaaagagtc atctaaaatc agtaatgctg gcttggcaat caaagctctt gcaatcgtca
    25921 gtcgctgttt ttgtccgcca gagaaatttt tcccattttc catgactttt gtatctaaac
    25981 catttggaag ctgacggaca aattcttctg cctgagcgat tttcagagct tcccaacatt
    26041 ctttttctgt ggcgtctttc ttaccccatt gaaggttttc acgaatagtc ccggtaaata
    26101 gtacagaagt ttggggaaca agagcaattt gctgacggag atgatccagc tgccaagaac
    26161 gaacatccat tccgtccatt ataactgtac cggaactgac atcatagaaa cgcggaatca
    26221 attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat gattccaagg actgtatttc
    26281 gcttcagctg aaatgtaata ttttggagag aaagaccaga atcaggagta taacggaagt
    26341 caacatggtc aaaagcgata attgcttcag aagaaaaatc gggagtagta ctgacctcag
    26401 cttcaagaat cgaattttct gtgtctagca cctctttcac ccgatttcct gaagcttcgg
    26461 cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag tgccaaaagc atttgattca
    26521 tataattgat caaagcaaga acttgtcctt gttgcaagct accaatattt actttgatcc
    26581 cgccaactgt cagcaaacag atgatcccga cattcatgat caaggttgta gcaggtgata
    26641 atagggcaga gatattggaa acacgtaaat aattgttcgc tagatcattt gtgcttttat
    26701 cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg gatgacacga acaccgctaa
    26761 gattctgact aacgtgttca ttgagctgat ctagtttttc ttgcactttt tggtagagag
    26821 gaacggtttt tttgatgata aaaaataaaa tgattgagaa aataggcaat aatgctaaga
    26881 aaaacagacc tacttcccag tcgatcacaa aagccatgac cactgacccg atacttaaaa
    26941 acggtgctct gatcaataaa cgaatgacca ttgctaaagc tagctgaagc tgattgatgt
    27001 cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg attcaactcg ttgtgagaga
    27061 aagtattgat ttttttgatt agctgattac gtaactctgt accaaatcct tgtgaagcta
    27121 ctgaagcata gtattggcaa atgatagcac aaatcagacc aatcacagac atagcgacca
    27181 tccataagcc catttcaatg atttttcctc ggtcattcat ttttagtcca ttatcgatca
    27241 aggaagccat cattaaaggc aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg
    27301 ggcccaaaat gatttgctta cgatagtctt ttgcataacg taataactga atcatatgat
    27361 tcctcctatt atttatttgt ccacaattga aaaaacaagt actaccacat tgtagcttag
    27421 gaaatttaga atgaaaagag aacaagcctt t
//
LOCUS       DADXTG010000024        27423 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.024, whole genome shotgun sequence.
ACCESSION   DADXTG010000024 DADXTG010000000
VERSION     DADXTG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.024"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..1051)
                     /locus_tag="IWI57_000897"
     CDS             complement(107..1051)
                     /locus_tag="IWI57_000897"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBC2449059.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1347..2149)
                     /locus_tag="IWI57_000898"
                     /pseudo
     CDS             complement(1347..2149)
                     /locus_tag="IWI57_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2370..3584
                     /locus_tag="IWI57_000899"
     CDS             2370..3584
                     /locus_tag="IWI57_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2449060.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3606..5621
                     /locus_tag="IWI57_000900"
     CDS             3606..5621
                     /locus_tag="IWI57_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBC2449061.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5704..7077)
                     /locus_tag="IWI57_000901"
     CDS             complement(5704..7077)
                     /locus_tag="IWI57_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBC2449062.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7103..7387)
                     /locus_tag="IWI57_000902"
     CDS             complement(7103..7387)
                     /locus_tag="IWI57_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2449063.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7389..9404)
                     /locus_tag="IWI57_000903"
     CDS             complement(7389..9404)
                     /locus_tag="IWI57_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBC2449064.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9583..9888)
                     /locus_tag="IWI57_000904"
     CDS             complement(9583..9888)
                     /locus_tag="IWI57_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBC2449065.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(9977..11008)
                     /locus_tag="IWI57_000905"
     CDS             complement(9977..11008)
                     /locus_tag="IWI57_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2449066.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11215..12090
                     /locus_tag="IWI57_000906"
     CDS             11215..12090
                     /locus_tag="IWI57_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBC2449067.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12242..13084)
                     /locus_tag="IWI57_000907"
     CDS             complement(12242..13084)
                     /locus_tag="IWI57_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2449068.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13176..15053)
                     /locus_tag="IWI57_000908"
     CDS             complement(13176..15053)
                     /locus_tag="IWI57_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBC2449069.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15050..16471)
                     /locus_tag="IWI57_000909"
     CDS             complement(15050..16471)
                     /locus_tag="IWI57_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBC2449070.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEELRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16474..19863)
                     /locus_tag="IWI57_000910"
     CDS             complement(16474..19863)
                     /locus_tag="IWI57_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBC2449071.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20120..21511
                     /locus_tag="IWI57_000911"
     CDS             20120..21511
                     /locus_tag="IWI57_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449072.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21584..22231)
                     /locus_tag="IWI57_000912"
     CDS             complement(21584..22231)
                     /locus_tag="IWI57_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBC2449073.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22239..22925)
                     /locus_tag="IWI57_000913"
     CDS             complement(22239..22925)
                     /locus_tag="IWI57_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449074.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22931..24004)
                     /locus_tag="IWI57_000914"
     CDS             complement(22931..24004)
                     /locus_tag="IWI57_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449075.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24226..24900
                     /locus_tag="IWI57_000915"
     CDS             24226..24900
                     /locus_tag="IWI57_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2449076.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24905..26245
                     /locus_tag="IWI57_000916"
     CDS             24905..26245
                     /locus_tag="IWI57_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2449077.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26394..27380)
                     /locus_tag="IWI57_000917"
     CDS             complement(26394..27380)
                     /locus_tag="IWI57_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBC2449078.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7984 a         5188 c         4995 g         9256 t
ORIGIN      
        1 accattctgt ttatttgctt acctattaga tatcaataaa aaactgctta acagctattt
       61 cagctgttaa gcagttttag gatttttagt tcatattcat tgtttcttat aatacagtgt
      121 tcaatacttc tcttaaagca cttgttgatt tgctgaacat tgctttttct tcttcgttga
      181 ttgataattc aacaacttca cgaacaccat tttcgtcaac gattgatggc acacctgtaa
      241 agatatcttt ttcgtcgtat tcaccagtta aataagcaga tacaggcaat acagcttgtt
      301 cgttattcaa gatagctttt acgatacgtg cagtactcat accaatacca tagtaagttg
      361 cttttttacg atcaatgatc tcataagcag cattacggac tttatcagca atcacatcta
      421 attcatcttg ggcaatacga tggtcttttt ctacaatttc aaacactggt ttaccaccga
      481 cagttgtatg tgaccatgca gcaacttctg aatcaccatg ttcacctaag atatagccgt
      541 ggacgctgcg tgggtcaact tttaatttca atgcgatttc tttacggaaa cgagttgtat
      601 ccaatgttgt tccagtaccg ataacacgtg atgttggcag accagattcg ttccaagcga
      661 tatatgtcaa aatatctact gggtttgaag caacaacgat aataccgtcg aatcctgatc
      721 ccatgatttc ttttacgatt tggcgcatga ttgacgcatt tgttttaact aaatctaaac
      781 gagattgacc aggtttttgg ttgataccag ctgtgataac aacgatatca gcatctttac
      841 attcttcata gccacctgac catacagcca cattttcatc gccccaagcc ataccatcta
      901 aaaggtccaa tgcttcccct tctgcttttt cttgattcac atcgatcaat actaattcat
      961 tagagattcc ttggttgatc attgcataag cgatacttgt tccaacaaaa cctgtaccga
     1021 cgattactac tttacgactt gtttttttca tgaataaaca gctccttttc ataactattt
     1081 gttactttca tgaaaagtat agtcttgttt attcatttta tcaacatgtt aattataata
     1141 gttgttatta taatttttga tggaaacgaa ttcctgaata cgcataacag ataaatgaca
     1201 aatgtttaca cctatatccc ctgataatac aaccaaaagt ttagaactga aaataaatcc
     1261 aaaaaaattc gctgcaacga tataataaga cagcaatcgc acctaaaaca attttccttc
     1321 atatgcttta gtgaaagatt ttcttcttaa tgtttatttc tccattcatt gggagtacat
     1381 gttgaatact tcttgaataa tttgaataaa gaagtcgttt gtttatatcc gatacgatta
     1441 gcgatttctt ccaacgaaaa actcgtttct tgaatcagtt ccaacgcaaa gtcgaatttt
     1501 ttctttagaa ccagttcttg aaaggaagca tcaaaataca ttttcaattt attactaaga
     1561 tagaccggat tgtagccaaa atgagctgct gtttctttta acgtacaaga agtaaaatgc
     1621 ttatcgatat aatctaatac tctaagcatc tcattttctg cgcatccatc tgctgatgaa
     1681 aagctttgtt gttctaataa atgagtcaat tcgatcagta atagtgatgt atagagattt
     1741 aacgaacgga tcttatgact gtatgtatca aaataaatca acagcatatt ttcaatcaaa
     1801 tgcgtcaaac gacttgtttt tccgccgcga aaatgaatga attgtgtatg ttcccctttt
     1861 tcttttgatg catttatcaa aaaagaagaa agtagattat ttgtgcttgc caaatgattc
     1921 aatatctctg tagataaggt ttcttctttt aataagatgt tgattaaaat atcctcttgc
     1981 cctgtgtatt cgattctttg aggtgtttac tgatatgaat atttccctgt tgaaatagat
     2041 cccctcggtg gatttttggc ggtgtctgaa gatggtaagc taagtttaaa tcattgatat
     2101 tcattccttg ttcacgctgc agagtttcga ttgattcttc gttacgcaaa aaaaatgtct
     2161 aattcagcta gttccaattg actccctcct ttttgtaagc ggtttctttc gcttattagt
     2221 tcacgctgta ctgctattat atgcaaagct atttttggtt tcataaagac tttctatggt
     2281 tgggacatga acttcctgtg attccgctat tgagagaata accacttcat actatatttt
     2341 tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa taaaaagatg atgaaatttt
     2401 ctctgctgtc agtctctctt attttgactt ctgctggtgc agtcacgtct gcaattccca
     2461 gtatgtcagc tacttatgcc acgcgttctt tatctgctgt tgaattatta acgactgctc
     2521 cctcttttat ggtcactatt tttgttttga ttagtagttt tattgctagg aaaatcggac
     2581 aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat ttgtggtatc ctccccctct
     2641 tctcacaaag ttacagtatt gtcttagctt caagagctgg actaggtatt ggctttggat
     2701 tgatcaattc tttggcagtc agcatgattg ctgcctttta tcacggagat gaacgagcct
     2761 ctatgattgg ttttcaaagt gcatttcaag gatttggtgc agctttaatg acatttgttg
     2821 caggacagct agtacagttc ggttggcaat ataccttttt agtctacgcc atcagcttac
     2881 ctattcttgt cttgtttgtt ttgtttgttc ctacacctga aacaactgaa cttcaatcgc
     2941 atggcacagc cccaacaaga caagtaatca aaacgaatcc taaaatgatt agttatggta
     3001 tttttctctt gctggtcatc gtggtatata atgctgtatc gattaaatta gccacagtcc
     3061 tgacgactta ttctctagga aacgaaacta atgcagcgaa tatcttaaca atcatgcaat
     3121 tggcaagtat ggctgccgga gtttgttttg gctggcttca gacaaaagca catcgctata
     3181 tgcttacact ttctttacta ctgatgggaa tcggctttgt attgattgcc actgtgataa
     3241 acctgtatgt gattgggttt ggtgcgatcc taacaggaat ttctttttcg ttattcattc
     3301 cttacttatt caaccaagta agtatccaag cgcccaagta cgcggagagt ttcaatactt
     3361 caatcttatt ggttggcgct aatttaggta ctttcattgc accatatggt ttgctctttc
     3421 tctcttttgc ttctcttggg agtaaaacac ttgctgtttt cgtcaatggc agtatcttgc
     3481 tgttgattag cgcgcttatt agccttctag tagtacacag gacgaataga aagacagtga
     3541 taagagtaga tgaagaaata aaagaacaat tgacaacaaa ataaatttga tggaggtcgt
     3601 gtaaaatgac aaatgaaaaa cataaagata gatggctttg gtatcctgga gactttgaga
     3661 tccgccatgg actgctacaa aatttccaac gggaagaacg aggttttgac tggccagcgt
     3721 actggtacat ggatgattgc catcggaacg tcaagtttaa gagatattac tttcttgatc
     3781 aaccttccat gtttaaagtg acgatccaag gagttgggta tgtagaaatt aatggacaaa
     3841 aacatccatg tggaaaatgg ctgacttgtc cagctggtaa agcaaaaatt cgtatctttg
     3901 taggacatac atcagggcta ccagcgatgt ttatcgaggg agacgaagta aaatcagata
     3961 ttggctggac agcaagtaat tttattgaag aatatccagc tggctggagt ccgctttatg
     4021 tcgatatcgc aaaagatcca aatcaaatct attatcaaaa agaatgcgtc catccagtca
     4081 ctgaaacgga gattggcgga ggaatcttat tcgattttgg gcgagcagtg aatggctgtg
     4141 tagaactaat atctctggat gaccatatcg aatctatcac tctatgttat ggagagtccg
     4201 atgtagaagc tatggatatc gattattgct actataagca aataggaatg acaagaaaaa
     4261 tgaaagctcg taaacgcgct tttcgctatt tgtttatacc agaggtttct actggaatgg
     4321 ttcaacttca agtttttcat gaatttttat ctctttcgaa acaagctgct ttttcaagcg
     4381 attcttcttt actgaatcag atctggtcag tatcagaaga agccttcaag ttatgtagtg
     4441 atttgttttt tatcgacggt atcaaacgag atcgctggat ttggtctgga gatgcgtatc
     4501 aatcattcat gatcaatcaa tatctttttt ttgacgagga aatcaataaa cgaacgatat
     4561 tagcattaag aggacaaaat gaaatcaagc agcatatgaa tactatcgtt gactattcga
     4621 tcttgtggtt gattggtatc gaaaaccact atatgatgac gaaagactat gaatttttac
     4681 gcatcgtcta ccctaagatg caatcattaa tgagatactt gatggagcaa acgaatgaat
     4741 tagggtttat ttatgggaga gcaaaagatt ggatatttat tgattggtct gaaatggata
     4801 aagaaggaac gcttgctgca gaacaagtct tattactgaa agcctatcat tccatgatca
     4861 cttgcggaga tatcttagct gaaacatatg gagagaacga aaattatcat actcatctct
     4921 atcaagaaaa atatcaacta ttgaaacaaa acctatttga ctacttttgg gatgaagaac
     4981 ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa tgtgacgcgt catgcgaata
     5041 ttttcgctgt tttattcgat tttgctaaca aagaccaaca gcaatcgatc ttaaagaatg
     5101 tcttattgaa tgacactatc acacagatta ctactccata tttcaaattc tatgaacaag
     5161 atgctttatg caaactcgga cagacgaata tcgtctacca aacgattctt gattattggg
     5221 gagggatgtt atctcatgat gcggtaactt tctgggaaga atacgaccct actcaaacag
     5281 gaaatgaaaa gtacagtatg tatggagatc cttatggaaa aagcttatgc catgcatggg
     5341 gagcaagccc aatctatctt ttaggacgct attttatcgg tttgtatcca acaaagccag
     5401 gttatgaaga atttatgata caacctaatc tagattcatt tgaaacactg aattgtacgc
     5461 tacctatcaa agatggaaag atttcgcttg aaaaaacaaa agataagatc gtgattactt
     5521 ctgatagaac aggcggaaca gtgaaaatga atggtaaaag ttatcctctt ctagcaaaac
     5581 agccacttgt cttaacaagc tattcaaaaa taaatgcata aaacaaaaaa ctgcttgcga
     5641 catatcagaa gactttaaat cttcttctga tattgtccaa gcaattttta ttttgaagtt
     5701 tgcttaattc tgttctttag aaacaatgtt tgttcttttt ccggcaaaaa gaaagctaga
     5761 taatacgaca agtaaaaaga aagcgatgat tccaactgtc aaggctattt gaccgtattc
     5821 tcctagtttc ccaagtacaa gtcctggtac tagataatca gtatcggcaa atgtagaaga
     5881 agcgatttta agatctccta atactggaag gataaataat ggcagccaac taatcaataa
     5941 gctattgaaa gcagaaccta ataccgcccc tcttctaccg ccagtcgagt tgccgaagac
     6001 accagaagca gctccgcaga agaaatgtcc tactacccca ggaatgataa ctgtagtttg
     6061 caagcctatc atagcaaaca tggatagtgt tcctacaaag aagctaacaa aaaagccaat
     6121 taatacagca ttaggtgcat atggaaaaac gataggaaca tctaatgctg gtttagaatc
     6181 gggtacgagt tttttagcaa tcccttggaa tgctggaacg atttctgcca acaccattcg
     6241 aaccccttga aggacgataa caaacccagc tgtgaatgtt ccagcttgga tcagtgcaaa
     6301 gattacagcg ttgtctccat tgcttagttc tctttcaaca aatccaggac cagctaacaa
     6361 agcaagtgcg acatatacga tcgacatcaa gagcatgatc gatacagtag aatctcgcaa
     6421 aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa cggtctttgt ttccaaaaaa
     6481 ctttccgatc catccactag aagcataacc gatatttccg gtatgcccta acgcaacttg
     6541 attgtttcca gtgatgttct tcataaacgg ttgacaaaga gcaggcgtaa tagttaatag
     6601 tgttccttcg aagaccccgc ctaataagat catccacaaa tttgcagtaa accccgcgct
     6661 tactaaaatg actgctgtta gacaagaaac ataaagcatt gcttgacctg taagaaaaat
     6721 gaatttatat cttgtgaatc tagcaagtaa aatattcacg atcatcccta ctagcatgat
     6781 caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc actactgctt cattgctagg
     6841 aactactcct tgagtatgca gagctgtttg gaacatcgaa gcaaaagggg ttagtgaatt
     6901 actgattaaa ccagcaccgc cgatcagtac taggaatccg acaaatgttt tgatagttcc
     6961 ttggatcact tctgaaaatt tcttcttctg aagaacgaga ccaagcagtg caatcaaaga
     7021 aacgaggatg gagggctgac tgacgattga cacaaacaaa ttcaacaatt tatccattga
     7081 tagttcctcc cttgaatcat ttttattctt ttgacaagat cttcgttact ttttctttta
     7141 cttcttcgct gtcaatgatc gattgcaaag gtacgagctt ttcatcaggt atagaagtta
     7201 gcgtatcttt caaagaatgc tcaatgaaga aataatcagc catatcgcta gatacacttc
     7261 caacatccgt atgacttact tcaaactcgt ccacatctgc tccaagatct gcaagtgctt
     7321 gtttgatgtt catctctacc ataaaacttg ttcctaatcc actttgacat actgcaaaaa
     7381 ttttcatatc attcgtctcc ttttcttatg atcgctaata tttcttcttt tgttgatgcg
     7441 tgtaataaat cttgaagatt ttgtttatta gaaagtagct ttgttaaatt tgccaaagcc
     7501 cgaagatgtg cctcattatc gatagcagct aaacagatga acaaactaat gggatgggct
     7561 ggattatcaa gcagatccac aggttgttga agtttcagca aagacatccc tacttcgttt
     7621 acgccatctt ctggacgagc atgaggtaat gcgatgtgat cgccaatatg gataaacgca
     7681 ccatactgct ccacgcgctc gatcatagct ttgatataac tttcttctat tttattttct
     7741 gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc agttttttgg ttgatccgat
     7801 aattggatca tttcttctgt taataattca gatagcatag gacgttgatc ctccttcttt
     7861 tctaaaagtt tgttcatttt acgatttaat acttgataga gcttttcttc gtctacgcct
     7921 tcttttaatt cgatataagg cagtagcgcc gacaacaatt cttgggcaga aggcattgaa
     7981 aatcctggta tcagccaatc tctttgtact tgattgatca gctgattttt ttccaagtca
     8041 gtcatgattg gatgcagaac atagagagac tttttcttat cagtaagtgt gagaggtaca
     8101 gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt gtaagcggtc agctgaatta
     8161 gtctctgtaa aaaagatcat cgggaataat tgctgaagtt cactttttaa gatcaatgaa
     8221 gagctaatgc cacttggaca gacgatcacg gctcttattt ttctatttct ttcttctgcg
     8281 tccgcttttc tgatctctcc gccaaatagt atagtgaaaa aaccaatctc ttcttcaggg
     8341 atgctcttcc ctattcgtct ttctaacgga agtaatgctt ttcttgtcat ttcgtagatg
     8401 gaagcatatt gatgcttaat ttgatcgatc atcacattag gtaaataaaa agaataggaa
     8461 atacgaaaat aggcagggac caagtgataa aagacattca tcaataattc acgaaacgaa
     8521 gcaaattcta cggccatcag gaattccatg cgatggatga cttctgaaga acattccagc
     8581 aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga tcgatgctaa gcaaagagag
     8641 aaaaatataa tctccgaatt ttgatgttcg gaatagccaa ggccagtcaa tacttgtcga
     8701 ctaatgtcag tgactgcaag agaagccaac agttcagcat cttttaccag ttccatggat
     8761 gtttttccaa ttcgaatggt tgtcaaactc aaataataag ctaattcatc cattcgattt
     8821 ggtgcgaatc gaagatctga tagtgaaatc gttttatcta gcaaatctct aactttggcg
     8881 taataatcaa tggaaagggt tgcggttaaa tacgtgagcc caaatcttcc agcaagtgac
     8941 tgcatcaaac gagcagtgcg attttttgct tctcttctta atattgcttc tgaccctagc
     9001 agcctaaaac cagttttcct agtatacgac agctgacaat cttccacttc taattcattt
     9061 cttatctttt tgatatctga caggactgtc cctttagaaa cctgaagaaa atcttgaaaa
     9121 tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca aataaagaag atctcttcgc
     9181 tcttgttcgg tatacacagc ttcaaatcga gtaatatcca aaaatttttc tgcccagtcg
     9241 attgctcgga atttctttat acggatacgg gtatcatcca catattcggc aaaaggagtt
     9301 aattgtttga tcgcctcgct tgtatttact tcgatgatct ctgctgcttt ttctagactg
     9361 atttctcttg gtgttttcag gagataacta attttgtagt tcacagcgct gttcacctcc
     9421 tacttatatt ctagtatggt agcgctttta aatcgagggg tgtttggtac aacttacagt
     9481 tggatttttt ttacgactgt accgaacgat ttctatcgaa tggatacaga aaaaaagcag
     9541 gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc gatcaatgat gatcatgttt
     9601 attttttgag gtcagttctt caaatttttc tttgacatct tcaaccgctt tttcgatatc
     9661 tttttcaaag tgattgggct cattaggctt tgtttcctct tctacagaat ttgaaccaga
     9721 taaagctgtt tctttatttt ctgtttcacc agagtttgtg ccagctaatg attcttcttt
     9781 gtcttctgtt tcaccagaat tagttcctgc taaagccact tcttcttctg ttttaggggc
     9841 cattgaacct cttaatcgtt tgtgtttttc accgtcataa tttgtcatgt tggttcctcc
     9901 tagtagttct atactttttc atcgtacaat aagacagttt atgcgtcaaa aaacaaaccc
     9961 gattcttaca aatatgtcat atttttcctt ctgattcttg aatgcttttg attccttctt
    10021 ccaaaatttt catttgtaag attgtttctt catctttgat gactttttct gcgccattaa
    10081 ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc accaacctcg gaaattactt
    10141 tttcttcatg ataactccct tgttcatcta tataggtcaa tacaccataa tgttcgggca
    10201 aatcgatacc aaagtcttca tgatcaggca tgtaaaacat tttcagatgt tcttcttgtc
    10261 gatcttttgt ttgtttcacg aacatccctt tttttccgta aacgacgaaa cttggacgtt
    10321 ctttgatgcg gaaataactg gattttatcg aaattttcat atttccatag aaaaaatcca
    10381 gatcgaagta atcattcatt cgtcctttcc ctaaaagttg acgtacatca tagctgatat
    10441 catctggttc gccgaaataa gaaagaactt gatctactgt atgacaagca tgtccatata
    10501 aatagctgtt cgcagtagaa aattcatgcg tattttctgg tacttccggt ctgaaataat
    10561 cataatgcat ttctacttct aaaatatccc ctaattttcc gctttcgatt actttttgga
    10621 ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca ttggataaat agaccttttt
    10681 cttttgctaa tgcaaataac tcttctgctt cctttgacgt ttctgcaaaa ggtttctcta
    10741 ctagtacatt cttaccatgt tcgagtgcca tttttccata ttcataatga aattgactag
    10801 gtgtagtgat gacaactaat tggatctcag gatcatccca aagatcagac agctgatcag
    10861 tatattcgat gtcttcgaat gttttccaca ctggttcaga tggggaaaaa atttttttga
    10921 tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg atagcgattt gtactctttc
    10981 cattgccaag ataagcgatt gtaagcatga gtggtgcctc cttttattga tgttttcagt
    11041 atatcctaga aaagcgcaga aagtatttat aatagctatt ctggtaaaaa agttcagaaa
    11101 aacacttgtt atcattcggt ttggtgaaat taatgttcaa agtaaggata aaaatcaaaa
    11161 agatagtagc ggaaccaagt gtttatcagt aagatagaat ttggaggtca gatcatgctt
    11221 attgaagaaa aactaacaaa acaagagttg tttaccacga cagagaaacg gattgtcgat
    11281 tacatccgta ggaatatcga agctgcagtc tacatgacga ttgaagaatt agcaaaagct
    11341 acgtatactt cccattcagc tatcattcgt ttatgcaaaa aaatgggatt cagcggattt
    11401 aaagaattcc gtttcgagct ggggcgggaa gttcatcaat tgattgctca gttcaatcaa
    11461 acgattgatc ctaattttcc tttttcaagt gaagatagca ctcagcaaat tgcaaagaaa
    11521 atggcggatt tatcgatcca gtcaataaaa aaagcacaat tgcagattga acatgaagat
    11581 ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa tctttctttt tgcaaaaggt
    11641 gattctcaaa tcacggcaag gaaattccag aataaaatgg tcaagttgaa taagtttttg
    11701 atcatggctg aagaatacag tgattcttct tggaatgcag ccaatttgac tagcgaggat
    11761 tgcgccattt ttattagcta cagcggtcgc atccatcatt atgaacgaat aatgacctat
    11821 ttgaagcatg tagaggcccc taccttgctg attaccggaa atcagcattc agaaatggcc
    11881 aaacaggctg atatgtgtct tgttatatcg caggaggaat atgattttgc taaagtagcg
    11941 actttctcct ctcaaattgc cttcgattac gtattaaata cgttattctc agtgatatat
    12001 tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa
    12061 ggccttctta aagaatatcg gaaaacttaa gaaaagatcc tcatttagta ataagaaaga
    12121 tagtgtttga taggctctct ttgatatcat cataaaaaca ggctgaagtt ttctttttca
    12181 aacctcagcc tgtttgttta cggaacgatt cgaatagata tattatagaa atgaggaata
    12241 gctaacttct gtttcttatt ttgaacgatt gacgaaaaag atatataaga aggaggaaaa
    12301 tcagtataca tgcagctaac aatccataca ggaaaaatcg tttgttctcg gatactttct
    12361 gtacttcttt ttgaacttct tttgcatgat aaggaacccg gtgccctctg acaagtaagc
    12421 gatgactgtt gaccatataa ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt
    12481 cgattctcag atcttttgta tcagtcggtt ctaccgtctt gatctgatcg acttgataag
    12541 cgagtgtccg tccattgatt tcgatataga aagaatcacc taaagtcagt ttcggtaagt
    12601 ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga aataacggca tgtgtatttt
    12661 ctccccccgt aggataagac gttccttcca ataatgaggc acctttttct agcagcacag
    12721 tattcgtttc atcaaaaatt ggcaaatgga cattgatctc agggatcgtc aaaataccga
    12781 tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg ttcttttttc ttagtaaagg
    12841 gatctgcacc tggatttcca ccttcttttg ctaattgctg gtttttcttt gtcatgtttt
    12901 cttggatttt agccatgact tcttcatttt ctttgaccgc ttgttgctga taatgagaga
    12961 tgatctgctg atccagataa ttattcagcg cgtcgctaac aaatggataa gctagtatcc
    13021 ctattcctga gagaagcaag aaaagcatca gcagatctag aagtttcctt ttctttggtt
    13081 tcatttgtgt tctccttgtg atgactgttt gataataaaa gaaaaaggag catttgcgta
    13141 atccgctgct cctttttcca attcttgcaa aaagactaaa tctgactgtg cttgcgtcta
    13201 gcaaagtaca gtccagcaat cagtagaagg actactcctg caccgatata cacatagatt
    13261 cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa
    13321 atcaactgtc ctgctgttac ataagattgt tcatcgatag taaatgctac acggtttgtt
    13381 aatggaacat atttttctgg cgctttcgtt tcttccagat agtacgtgcc atattttaga
    13441 cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc
    13501 gatacccagc tgacggcttt tgtggatggg tcgatcgata aatatttcgc tgtgtcacta
    13561 tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt ggtctgatgt aacgtcacca
    13621 tctactttaa caaatcgttt gcccccagta acgacatcga ctgacggtgg tgtttgatca
    13681 tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc ctttggttgg atctgctttt
    13741 tcgttcaaat gcatatagta aacgaatttc aatgtaccgc caggagttaa tgaaggaata
    13801 taattcggat caactgaaac cgtgaatccg tctgtttgct cagtgacaga ataatttact
    13861 gggtcgattt ctttatttcc atcatataag gcataagcat acgttcctga agaatcatta
    13921 tcaaatgtta aagcagcgtc atgagtatcg ataagtttga atgttgtgta cttgttttgc
    13981 gttccttctt tatcagcgat acctaatggg atattgacag aaatctcata ttgaattttt
    14041 tcaccgattg cgacatcttt tccattcaat tgaggtgttg ttttatcaac tttagacgta
    14101 tcatttttga tggtcttttc tactggtgtt tggctatttg ccaggatctc aaaaggcgtg
    14161 attgtttgct tttcaatcat ttccgcatta tcaggagctt ttacttcttc taaattatat
    14221 tttccaactt ctaaatgatt aacgatcaac tggccttttt caatagatgc ctcggcaaag
    14281 tcattgttgc cgatgtcata agaatgacca gtaatgaaat gtttggcttt ggtttgatca
    14341 gttgtccaag tgtacaatcc atctgtgaca ctttggatgt attttttgac gcttggtgtt
    14401 ccttcttctt tagaaataat aaattctgct ccatttagtg cttcgttttc ggcagtaccg
    14461 atttttgtaa ctttcaacgt tccatcatta ccgactgtat ttttagggta gagatggata
    14521 gtatctagtt cttctgtccc gtatttataa gagccatctg cttgtttgat catctcatat
    14581 acagggaaag ctaaaaccat gtttgcggca gctgacactc cgtcttttgg ttcttctttg
    14641 atcgtgtaga ctgcatcttt cccattttgt tttttaggta aagataaagt gacatttcca
    14701 tcagcatctg tcgttcctga agcaactggt gtaccaggag tcaaagactg gactgcttgt
    14761 tttgctgcat ccacggacgc tcctttatct cgttgcgcat aaaattcttg agtgacgtta
    14821 taaactgaaa atgaaatatc ggctaatcct tggtattgat cgaattcgct catttctttc
    14881 ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt tctttttatg aaggatgact
    14941 tgagctgctg tctcctctgc aaacactttt tttgcgccga agctgtttgt gagtaatggt
    15001 aaaataagga aaaggactcc taacataacg tttatttttt tatgattttt cattttttca
    15061 ctcctgttct ttttttgata aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa
    15121 cgcctaaaag tgtatttttc gtttcgtttg tttttggaag aaatggacgg ctattttctt
    15181 ttttattgac tactttcaat atactgtccg cctctttatc ctcagaaaga aaagttgaaa
    15241 tggtaaacgg aatttctgtt tcacttaaaa tataaccaga aggtgcttcg atctctttta
    15301 atcgataact tcctgttttc aacccggaaa ttgaaaaagc gccattttta tcggaaatca
    15361 ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc gttggctgtt tgctggaggt
    15421 aatttcctgc tttgttttta ataaggaata ctgcgccagc taatcgttta tctggtttat
    15481 ttgtctcgat tttgacaaat tgcttactcc ctgtatatac agagactttc tttgtactga
    15541 caccaaaacc aaaatccagt gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac
    15601 tgcttagctg catctgataa gaaatggtca gtttttttcc agcatatttt tccaacgaag
    15661 ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg tatgacaaag ccttctgtca
    15721 gcttttctcc attcgatgaa atcgttaaac tattaggtaa aaacgttaat gcttcatccg
    15781 cactatctga caattcgaac ttttgatagt caaggatatt tacaggaatt gtcgtatgca
    15841 aggaataagt gattttttca ccaatcgtaa aatcatttct aagctcttct aataccttct
    15901 caaaaggtgg atcagggtag tcgttttctt catttttagg atacaaatgg atagttgaaa
    15961 gtttttgatt gttttgtcca tgtacaggaa gaacaactac catattctct gccttttctt
    16021 tgacgacttc tggtactttg gattcaatga aaagatacgc tttatctctt ttttcttgat
    16081 ctttagcggc cagagaaaaa gaagcgatac catcttcgtt gttaagagtt gtagttgttg
    16141 cttctgcggt aaacataccg gaagacgcac cgttttttgc gatctctgct tgtgcttctt
    16201 ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc tgtgacatca taaacttgga
    16261 atgtgacacc atttaatcct cgatacgttt gtaataaagc tttttcttgt ccgtcatttg
    16321 gatgattttt cggcagttgc ccgtttggga aaagcaattt gtgtaaagta atctgaacca
    16381 tttctgtttc tgtttctgtt gctacctgag taaaagctgg tttaaatagt aacaagaaga
    16441 gacacatact aagccaacca agttttttca tatttagctg ccccctcttt cttttttcaa
    16501 aaaaagatac caaatagaaa acgcacaacc aatcatccct actagataga ttcctaaacg
    16561 gccaattcct cccgtttcag gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat
    16621 ctggttgttt ttggctcctg gtgacagaac agattcatga tcttgtccat ccacttgaat
    16681 tgacccatct tcgtgtataa ctatagtaac tggctctttt agaccttggt atccttctgg
    16741 tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa ttttcgaata gaaaggtatc
    16801 tgtttcttgt ccatcttttg gcgattctaa gtccatttct ggtccctgca gtctgaattt
    16861 tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt accgttagat caaacgcttt
    16921 tagtcgattt tgatgagtga gcgtccatac attatttgtc atctcggtat tttttcccgt
    16981 gtaaatgatt tttccatatt tatcgatttg gaattcataa tttcccggcg aagtatcata
    17041 tccggcagga ccagctgttt caacgactcg atatttacca ggtgtgagtg aatcaaaact
    17101 ggcaagacct ttttcattcg ttgtttggct gtcaattggc tgataagtac catttgcttc
    17161 ttttttctgt agggaaaatg ctgctccttt aagatttatc tctttgtccg tcccttgcat
    17221 cgcatactta cgtactgcta caggaacttc aacaaaagga ttttcaatag tcaactggat
    17281 cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt accgtaccat cagaagcgat
    17341 cttgatttcc caagtagtct ttttgcttaa tccatgccct tctggagctt ttgtttctgt
    17401 cagcgtatag gtcatttctt tttgcaattt gacattttct ggaagagaat aggttccgtc
    17461 gccatgatct gttagtaaag tatcaataga atctcctgtt aatttgaaaa cagcgccaat
    17521 aagatccttt tcacctgtag aggaattttt cgttattttc aagtttaacg gtgtgaattg
    17581 tttagtattt ttccatgtca ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa
    17641 ttctttgatt gtttggtaac tgaatgcttg accttgatta ttgtattgag gtagtgataa
    17701 tatctcttga taactttctc cgctatttgc agataatttg tcaatgtctg cacgttccca
    17761 cgtatttgtt gtatctttag ctggtttaat gattcgaata tatccgtttt tccaagctgc
    17821 agcatttgtc gtatgttccc gttgaatctc aaaagtaact tgatctggac gatcagctag
    17881 attgttgtca aattcttccc ataacttttt gatgtgaaga ctgactccgg gagctttagc
    17941 agatggtata ccgaattcag ctaattcatt ggtatcaatg tttggctgga aagttgtcct
    18001 gccgttcatt tgataccaga aatttggatg gaagtcctca ttttctgttt ggattctcac
    18061 ttggtaatgg atttggattt cttggtcttt tcctaaatag atctgattgc tcttgatggt
    18121 atttccttct atggaaatag atggagtgac cgttgtagga cttgtcccca cactcttgac
    18181 tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt ggatcattta tttgtccatt
    18241 gcttacagtt gctgagatct gtacagcttt tttggctagg tattcagaga tatctgttgc
    18301 atgatcagct gattcatagt aaagatcgcc tttttcatct gatgaaacca tttgtcgcat
    18361 acgagactct acttctgctt ttgagagacc agctgccgga tcgctttgaa gttggatgcc
    18421 aagaccatgt atttcgattc ctcgttcttt gagtgccatc gcttctccga tggttgcgat
    18481 aaacgtactg tcgattcttc tggatagatt gttttggtca ggtgcatcat agattcttga
    18541 gattagagaa gtatttcccg gccgatcttg cgtattagaa aactgagttc cgtaataatt
    18601 gctgcttgat tgtgcgtgta cccgctgtac tttataggaa aatgttggta caccatccgt
    18661 cagcaaaacg atcacttttt tatgtccatt tggaacggat agcatgcttc ctgcatctct
    18721 tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa ggtgtaatgg attttacttg
    18781 atttttcact gaatcaaatg aacccatctg tactgcaccg ttactgtagc tatatccttc
    18841 gcttgagtag ccgacatatc ccatattgat tttgtctgtg ataccgctat ctgctaaagt
    18901 atcgacaaaa cgatcgacac caatctttac ttcaccgatc cgattattgt cgttcatact
    18961 tcctgaccag tctacgacca atacgagatc aagaggcgtg atatcttttt gtacatttcc
    19021 acgagcattc aaataaacat caaacaatcc gggtgtactt gtttcctttg catactttcg
    19081 tatcgcatag tctgcttgat taccggttcc tccatattcg atatacgaat gggtccgatc
    19141 attaggatct ccattccaag aatttatacc atcccattga tttgttccgt ttttattgcc
    19201 ttgatgatca agaacatttg tatttcctgt aggttgccaa ctggctttag gataagtgcc
    19261 ctgatcatct gttgtatatt ctgtaggaat cgtttgatta gatggcacac tcacagctgg
    19321 caaggataat gtaggcaatg taaatgggct gacagaattt tcgttttctt ttgatgattc
    19381 ttcttctatt tcgtctggct ttggtactga cacatctgta tctttcttat tttctgtctc
    19441 atcaataatg tcatattctt tattatcgac gatcagcttg tttgcctggc tgtggtaaag
    19501 tgctggatcg attggaaaac tcagatcgat agtttgttta gtgtttgatg agatttccaa
    19561 cgtaattttc ccgctattta cctgataagc atagccatct ccttcttttc tttccacatc
    19621 acttaagtaa ttttcgacta gaccaatttc tttttctatc ttttcagcac tggtgttatt
    19681 cgcatttagc ttgatgttca gtgctttttt tccagactga tccgaaatga cttcagctga
    19741 aattgtttct aaaactgttg gatcatctgt agtgtctgct attgcagata atggcacaaa
    19801 gtttgataaa ctcaatatta tcagcatgaa aataactttc agtttcttcc ctgtggtcgt
    19861 catttttccc ctcctttttc tttttttctg ttttgacgtg aatagtgaaa attattaaaa
    19921 taaacaggcg ttacataatt ttatttattt taatatgata attattatac gaagggcgct
    19981 aaagggaatc acaccaattt tcataccaat tggtttcatt tatgaagttg attccttcga
    20041 ttaatgagcg tttcattgaa agaaaagggt tgagggagaa aaacatatga tcaggttgtt
    20101 ggaacgaaac tatagaaaga tggaaaaaat cattcgaatt ctgtccattt ctgacaagtg
    20161 gatgactaaa aaagaacttg ccaaacgaat tggtagttca gaatctacat ttgtaaggta
    20221 tatcgaagag atcaaaatcc gctggggaac ggatgtaacg attcaatctt cacaaaaatt
    20281 ggggtatcgt cttgaacatt tgaatgtacc agcttattta aatgttctga tggaaatgtc
    20341 gcaaagttct acaaacagtc gtttattaaa tgagattatt gtcaatccgg gtaaaacgat
    20401 tgagtattat tgtgaaaaaa tttccatcag ccggtcttct tttacacgta agttgaaaca
    20461 atgcaatcaa attttagaaa gctatttatt aaaagtgatc gttgaccaag gttttcagtt
    20521 aactagcctt aaaagtgagc tccagttgcg tatctttgtt acctttttct ttttaagtta
    20581 ctacggaaga cgcacgttgc catttccaat cgaaaaaaag aagatcaaag aaattatggt
    20641 caagaataat tgtcagatga atttgtttca ggaagaaagc ccatatgagc aatctttctt
    20701 tattatgtat tttgttgtgc atctcatgag agaatcccaa ggaactccat ttgaatggaa
    20761 agctggagac tcttatgtac agttgataaa tatcgataaa aaagacttct taatcttgaa
    20821 aaatctctta ggaaaattga agtacagcag ctatataaaa gtagtgaact attttgtcaa
    20881 tacgattttt tcagaatttt cggagaaaga aaaaaatgaa atcagtgagc agatacgaaa
    20941 aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa gaaaaaaacg aagaatatgt
    21001 tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt ttcccatacg atacgactca
    21061 tttgacgatc cgtgcaactt actttgcaca gcagatgcaa caccgccaga tacgtctata
    21121 tgctggattt cgtgcagaac ttcaatttta ttccactcta ctgaatcaga atctgattta
    21181 ttatttatct agctatatct tttggatgct tatcggtaat cctgaaattc accattatac
    21241 ttctgataaa aaaatactga ttatcagcga cttaagtctt cgacatagtc aatatattga
    21301 agaatatatc agtgatattc ttgctgtgca taaaattcac tctgagacaa ctgctattac
    21361 tgaagatcag ctgtcgaaat acaatcttct cgagtatgat ctgatcgtta cgaatcaacc
    21421 cattttgaat gcccatgttc ctcatatatt gattgatgat agtgtgtcat tcgcaaatga
    21481 agaagaactc atccgcttat tcgagttatg aataatcaaa aaacagctat gtataaacca
    21541 ccctgataaa aatttcagga caaatacaca tagctgtttc tttttattgt attccttcag
    21601 caatccaacg agatttatag cgaaagtctt ttaacaactc ttcttttgtc caattatttg
    21661 caatgagatc cgtcaaagtg ttcattacga gggtcatgga aaagtatagg aaactaactg
    21721 cttcttccga cgattgaatg gagaaatgat tgattcttaa taattccagc catttttcgt
    21781 tcattggaga atcagccgat agttctctac gtttggttgt ctgctgatca tttcgctggg
    21841 ttaacaagta taacctttgc caatagatac tttcgtgatc taagtcgcag caccactcta
    21901 aatatttttc gatccccaaa aagaaatgtt gtttgttttc gctaatgaat tttaagatat
    21961 cttgatggac tatgtgagaa ctgcttttga tcgtatacaa ataagcatct tccaaatctt
    22021 gaaaatattt ataaaaagca ccgcgcgaca tccccatctt gtcaacgatt tctgagactt
    22081 tgacttggct gatgtgctgt gagcaaaata tctccagtaa taggtcatcg attctctgtt
    22141 tctttgcgtt cgataaattg aaaaaagttt cttttggcat ccacatctct cctttatctc
    22201 tgattatacg tggatagccc ttcattgtca tgaaggattc attccttctt tttctcttct
    22261 tctgtcaagt acccatcttc cattcgataa acgcgatcac tccattgaat catacggaca
    22321 tcatgagtaa ccataatagt cgctttttgt ttctcatgag cttctttaac tagtaatttg
    22381 acgacttcat atgcatgatc tgtatccaaa ctagctgttg gttcgtctgc taagatcaag
    22441 cttggctcat tatataaagc gcgagcaatt gccactctct gtctttctcc accagaaagg
    22501 tctttaggat aactattttt aagtgactgg atatctaaag attgcagcaa ggaatcgatt
    22561 tttgttgaat cttctttttt tgaagtaact tggtcaacta gttcaaattg ttcctttacc
    22621 gttaaaaaag gaatcaaatt agagctttgg agaataaatc ctatttcttt aaagcgtaaa
    22681 tctgctcgtt ttttttctgg aagctcagtt agtgagtgac cgttgatcac tatttctccg
    22741 gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag tacttttacc tgaaccagaa
    22801 ggaccaatga tgctgacgaa ttctccttcc tttacataaa aatcaattgc tttcaaagct
    22861 gagacctttg tatgaccaga accaaatgtt ttggtaactt ttttcatcgt taatattttt
    22921 ttcattttta ttaacctcct attgctgtaa ttgggtctac tttggttatc gtacgtatcg
    22981 aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc actatatagc aaccattgtg
    23041 accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag actagtcaaa taagcaaaca
    23101 aaagagcaag cagaacgcct accaccccaa caataaagga ctgtgctact aaagatttag
    23161 cgatgaagaa atttttgatc ccttgtgctt tcattactcc aaaaatagca gtcttctgaa
    23221 gtgtcatcac gtacatgaaa ataccgacca cggcagcaac cactaagaac aaaaagtata
    23281 tcatcgcatt taaggtcata ttttgggcag aatagccagg aatactttca atgaatgtcc
    23341 caatagttaa taactgtaga cccttcgatg ctttttcttt cgaaatttta gctgaatcct
    23401 ttattacgat cccattgatc ggctgttctt cttcagaagc aaacggctgt tgtccatact
    23461 tcaaagcagt ccacgtatct agggacgcat agatgacggg agaaactgtg taataagttt
    23521 caggaaagat accgacaatc gtcaaaggat cgtcgtaact gccaattttg atcgtatctc
    23581 caattttata gcctgaatca gccagatttt gagagatgat gatttcattc tttgttttaa
    23641 attcatgccc tttcgtgagt tttggtaata aaaatgcttc tttcgttgtg ccgaaaactg
    23701 taacattttc tttgtttttt cctttgattg ctccgctgta caatccgatt ggtgctttct
    23761 ctcctccttc aatgtatttc agatctcctc ttgttaattg tgaagctgca aatacttgat
    23821 ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc atcgattgct tttttaaact
    23881 cttctgaaag tccattcgct agtcctgaca acatgaatac gacatacgca atcaagaaca
    23941 taataccgac gatcaagcca tatcgtaatt tagaatagcg aatttctttg atagctagaa
    24001 acatttgttt ttcctcccta tgatagattg atagtgacaa aatgtcactt gttgaatagt
    24061 gtaactctaa agtgacaatt tgtcacttgt gttgtttttt tcttatcaat aaattgtcta
    24121 agacatcttt cgataaaacc ttaacatgag tcataaaaaa tttatttaag ccttatttat
    24181 gctttccatg ataaactaaa gtgaccaaat caatgaggtg ggaaaatgat taaactatta
    24241 gtagtagaag acgataaagt attatccaat aatataaaag aaatactcgc tgcaatcggc
    24301 gagataacac aagtttatga tggtagtgaa ggactgttcg agttggaaag tggagtttat
    24361 gatcttgcaa tcttggattt gatgatgccg gaaatgaatg gatatgaagt gttagcaaaa
    24421 atacggaaag aaaaaataca gactccagta ttgattttga ctgcaaaaga tagtttggaa
    24481 gataagattg aaggatttga acgaggtgca gatgattatc taacaaaacc tttctatcga
    24541 gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct cgctaggtct ctttgatgaa
    24601 aatgttctga agtataagga tatcagttgt gatttaaaaa gcaatgaagt cttgtataaa
    24661 gaacaagttt tgcccataca aggaaaagag tttgaattgc tcgtttattt tttacaaaat
    24721 aagggaatca ttttgacaaa agagcagata tttgaccgga tctggggatt tgattctgat
    24781 acgacgatta cagttattga agtttatatg agcaacttac gaaaacatct gcgaagtact
    24841 ggattggata aagaaatcaa aaccttacgt aatgtcggtt atatactgca aagggtgtga
    24901 tgcaatgaat ggagatatta cacgaaagca acgtagtcgc ttttttgcga caaatgtagc
    24961 agcatttgct tttatctttt ttcttttggg attcattact ttacagatat taaaccaatc
    25021 tgcatacaga gaaacagatc tgtcattaga gaaaataaat tttgattctc gaacagtaca
    25081 attagagata caaagatatc agcaaggcga tcccttttta aacccgactc aaacggaaca
    25141 aaacagaaca aacatggaac agccattaat ggaaccatca aatgaccgtt ttaacactca
    25201 agtgatcttg tggtctaaaa atggtgagat attgaataaa gcagctattg gtgggcgttt
    25261 caaccaaatc aaagaattaa aaatggatac gaaaaatctg aataccatcc aaaatattga
    25321 attaaatgaa gacagtggct catctgccct ctctttccgt tcgattacga aaaaagcgga
    25381 gacgaatcaa gatggcgttg cctacatcca aattttagaa aatacaaatc aaatcaagaa
    25441 ttcattagcg acttttcgca cgattatgat tttgtgtatg attgtttttt ggattctctc
    25501 tattggtatc agttattact tgtctagtct aagcatgcgc cctattattc tttcttggag
    25561 aagacaaaaa gaatttgttg agaatgcttc tcatgaattg cgcacaccgt taacgattat
    25621 tcaaaatagt ttggaacatc tttttaccaa gcctaatcat actattattg aagaatccga
    25681 aagcatcgct caagcattgt cagagacgag gcgcctcact ggattgactt cggatttgct
    25741 gacaatcgct cggaatgatt ctgatcagca gctgctttca aagcaaatga tcaatacaca
    25801 agaatatatc gaacaacttg ttaaaccatt tcaagagatg gccatcatag atggaaaaga
    25861 gtttattctc cataacgatg caacattaga agtaccaatt gatgaaaaaa aaatccatca
    25921 agttttggtt attttgctgg ataatgcatt aaaatatacg aatccagaag ataagatcat
    25981 tttggattca tctgttaacc ataaagaatg gctgattcac gtaaagaata ctggcccttc
    26041 tatcagtgaa gaagatcaag caaggatttt tgatcgattt tatcgggaag atcaatctcg
    26101 ggcgaaagag actggcggat acgggcttgg cttggcaatt gcaaaacagg taatcgaaca
    26161 gcatcatgga cagatttctg tatccaatta tcttccacaa ggagtcgatt ttaaagtcca
    26221 attgccgttg aagcctaaaa aataatgctt atccctaaaa aagggctgtg acaaaaatct
    26281 tgtcacagcc cttttttgtt gcgaataaac ggtgttccag aagtagcttc ttcggaaata
    26341 agccgaaatt tctttaagct gaccacttct gtcacaacct ctttagctta cgcttacgat
    26401 tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt caatatccaa ttcttctcgg
    26461 ctaacatctt ctgtcacttt ttcaacatct tcatgttctt ctttatgaat cccaacttct
    26521 tcacgaataa ctgtatgttt gcttacatct acttgctctt ctttgattgg gatagtaatc
    26581 gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt gatctgttac tggtttgcgt
    26641 tcgatgacga tttcttcacg tttgaccggt acttctaccg tttcagtatc gttgaccaca
    26701 tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg tatttacatc taatttttct
    26761 tcttttagtt gaattgtatc atcatctaga tttggtttgc gatcgatgtc ttcagtcttg
    26821 cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg gatcagttcc tggtgtcata
    26881 ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac ctacgtccat tggatctgtt
    26941 ccagtgccag tttctaatcc agtccccatt gtgcttgctg ggctaacagt atcttgtgta
    27001 tccattgtat cttctgttgt ttctggctga cgatagcctt ttactgtgat gacaaatttt
    27061 ccatcagaaa tatctcctct atattgatat aaaggatcat tttctggtgt agccgtctca
    27121 gaatcatagg tatcaaaaga aaaagtatct tttatttttt cccataaaga acggtcttct
    27181 tcaccatcca tccgattttc atctgtttca acatctatac cagacaatcc gtcaaatcct
    27241 agtctagatg ctgcttcatc agtagtataa ataacgatat catctcgttc atacccttcg
    27301 ttacgcaacc gttcaattac tgataatgct tcttccctcg tactatagct tccgatgacc
    27361 gttaaatttt tctcttccat gtaaattcct cctagcaaat agatttatga ttggtatatt
    27421 gtt
//
LOCUS       DADXTG010000025        27360 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.025, whole genome shotgun sequence.
ACCESSION   DADXTG010000025 DADXTG010000000
VERSION     DADXTG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.025"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(13..777)
                     /locus_tag="IWI57_000918"
     CDS             complement(13..777)
                     /locus_tag="IWI57_000918"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBC2449079.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(778..1725)
                     /gene="prmA"
                     /locus_tag="IWI57_000919"
     CDS             complement(778..1725)
                     /gene="prmA"
                     /locus_tag="IWI57_000919"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBC2449080.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(1738..2226)
                     /locus_tag="IWI57_000920"
     CDS             complement(1738..2226)
                     /locus_tag="IWI57_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBC2449081.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDDQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(2192..2863)
                     /locus_tag="IWI57_000921"
     CDS             complement(2192..2863)
                     /locus_tag="IWI57_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBC2449082.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            3001..4287
                     /locus_tag="IWI57_000922"
     CDS             3001..4287
                     /locus_tag="IWI57_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBC2449083.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            4359..4553
                     /gene="ssrS"
                     /locus_tag="IWI57_000923"
     ncRNA           4359..4553
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWI57_000923"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4631..4915
                     /locus_tag="IWI57_000924"
     CDS             4631..4915
                     /locus_tag="IWI57_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449084.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            4988..5467
                     /locus_tag="IWI57_000925"
     CDS             4988..5467
                     /locus_tag="IWI57_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBC2449085.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            5690..5833
                     /locus_tag="IWI57_000926"
     CDS             5690..5833
                     /locus_tag="IWI57_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBC2449086.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            6411..7994
                     /locus_tag="IWI57_000927"
     CDS             6411..7994
                     /locus_tag="IWI57_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2449087.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKEMERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(8431..9615)
                     /locus_tag="IWI57_000928"
     CDS             complement(8431..9615)
                     /locus_tag="IWI57_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBC2449088.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(9640..10647)
                     /locus_tag="IWI57_000929"
     CDS             complement(9640..10647)
                     /locus_tag="IWI57_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2449089.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(10819..11178)
                     /locus_tag="IWI57_000930"
     CDS             complement(10819..11178)
                     /locus_tag="IWI57_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449090.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(11150..11521)
                     /locus_tag="IWI57_000931"
     CDS             complement(11150..11521)
                     /locus_tag="IWI57_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBC2449091.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(11538..11840)
                     /locus_tag="IWI57_000932"
     CDS             complement(11538..11840)
                     /locus_tag="IWI57_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449092.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(11857..12327)
                     /locus_tag="IWI57_000933"
     CDS             complement(11857..12327)
                     /locus_tag="IWI57_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBC2449093.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(12302..12580)
                     /locus_tag="IWI57_000934"
     CDS             complement(12302..12580)
                     /locus_tag="IWI57_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBC2449094.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(12577..13626)
                     /locus_tag="IWI57_000935"
     CDS             complement(12577..13626)
                     /locus_tag="IWI57_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBC2449095.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(13583..14512)
                     /gene="tadA"
                     /locus_tag="IWI57_000936"
     CDS             complement(13583..14512)
                     /gene="tadA"
                     /locus_tag="IWI57_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBC2449096.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14644..16398
                     /gene="ade"
                     /locus_tag="IWI57_000937"
     CDS             14644..16398
                     /gene="ade"
                     /locus_tag="IWI57_000937"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBC2449097.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(16496..17158)
                     /gene="trmB"
                     /locus_tag="IWI57_000938"
     CDS             complement(16496..17158)
                     /gene="trmB"
                     /locus_tag="IWI57_000938"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBC2449098.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(17228..18010)
                     /locus_tag="IWI57_000939"
     CDS             complement(17228..18010)
                     /locus_tag="IWI57_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBC2449099.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(18214..19425)
                     /locus_tag="IWI57_000940"
     CDS             complement(18214..19425)
                     /locus_tag="IWI57_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449100.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(19418..20155)
                     /locus_tag="IWI57_000941"
     CDS             complement(19418..20155)
                     /locus_tag="IWI57_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449101.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            20293..20727
                     /locus_tag="IWI57_000942"
     CDS             20293..20727
                     /locus_tag="IWI57_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBC2449102.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            20720..21064
                     /locus_tag="IWI57_000943"
     CDS             20720..21064
                     /locus_tag="IWI57_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBC2449103.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            21244..22254
                     /locus_tag="IWI57_000944"
     CDS             21244..22254
                     /locus_tag="IWI57_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2449104.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(22323..23267)
                     /locus_tag="IWI57_000945"
     CDS             complement(22323..23267)
                     /locus_tag="IWI57_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2449105.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(23251..25971)
                     /locus_tag="IWI57_000946"
     CDS             complement(23251..25971)
                     /locus_tag="IWI57_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2449106.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(25968..27188)
                     /locus_tag="IWI57_000947"
     CDS             complement(25968..27188)
                     /locus_tag="IWI57_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBC2449107.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
BASE COUNT         8265 a         5629 c         4806 g         8660 t
ORIGIN      
        1 taatgaagaa cgttagctga aagaaggatt cattaattcc cattgatagc tagcagcact
       61 taataaataa agaggtgctg tttctgtacg aaggattctt ggtcctaatc cacaaagtat
      121 ccctttgtgt tctacaaaat aatcgacttc atcgcgggat aatccacctt ctggtccaaa
      181 gacagctaag attcgactat ctggttttgt ttcactaaga attttggctg catttgattt
      241 ttcgccttgc tttgctgatt cctcataagc aaccaagacc acgtcataat catctagccg
      301 ctcaaataaa tcttgtttcg tttccaataa ctctactgat ggcacgatct gccgatggga
      361 ttgttccgct gcctcttgag caatcttttc aagccgttgt tgtcgtttca tacgtttttt
      421 tgcatcccat ttaacgacag aggctgatgc cgggaaacca agaaaagctt gcgctcctaa
      481 ttcagtggat ttctgaacga tccaatccag cttttcccct ttaggatagc cactcgctat
      541 ggtaatcgaa cttggcagtt ccttttgatt ttcttctttt ttaatttctt tcagataaac
      601 agcctcttca gtaatctccg tgatttctgc aacaatggct atttgatcat tgaatgccaa
      661 aaaaacttca tcctcgggtt ccatcctcat aacccgtaca atgtggtgat agttttcccc
      721 ttctaaaaca aaatagtctt ttgattcata tgattctttt aaaaaatacc gctgcatcta
      781 ttccacctct ggttttttca aaataattgc ataccagtct ttttgctgga aaatttgatc
      841 cacgataaac ccttgttcta acatggcatc cagaaccatt tgcttcttct cttcaatgat
      901 tcctgaaacg atcagcgtgc ctgtttgctt cagtaaacgc caagcatctg ggatcatcaa
      961 taagatgata tctgctaaaa tgttcgcaac gattacatca ctttcaatct cgattcctgt
     1021 caaaagatcg ttagctgata catggacatc tttggctatt tcattcatat ccatattttc
     1081 tttcgccgaa cgaacggcta cttcgtctaa atcaaaggca tagacatctt ttgcaccaag
     1141 atattttgct gcaatactta gcacgcccga cccagttccc acatctagca atgtttcacc
     1201 accacgcaag actgtttcta gtgctgtcaa tgtcaaggaa gtcgtgggat gagtgcctgt
     1261 gccaaatgcc ataccaggat ctagtgtgat aattttttct agaggatctt ctgtctggta
     1321 gttttcccag cttggcgcaa ccgtcagcat tcgcgtaata cgcaccggat gatagtattt
     1381 cttccaggct gttgcccagt tgctttcctc tacttcacta actgttactt tattttttcc
     1441 aatagctaga ccaaactcag gtaattgtgt gatacgatct ttgataaaag gcaagatttc
     1501 cggtaagaaa gtcgtttccg gaaaataagc catgactagt gcgccttctt tcagcgatgt
     1561 aaaatcattt ttatctaaaa tctcaccata ttgatccgac tgaaaatgtt caacatctaa
     1621 agaatcttca atcgcgacac cactcgcccc tgcctccatc aaaatattag caactgcttc
     1681 gactgcttca ctttcagttt ctacttttac ttcattccat ttcatatctg tcactcctta
     1741 ataacttggg tacggtaaat aagtatctgc ttcattttgc tgttttactt ctactaattc
     1801 tgctagttgc tgatcatccc agtgaagctc gaatacagct tcgtcatcgt cttcatccaa
     1861 aaaatcaagc aaatcgcttt gcccttgagc taatacttca tttagatatt ccactaaaga
     1921 atcagctact gattttgcca accctttttt tccttcataa gggattactg ctagataatt
     1981 gcgttcatca aaaacagatt tctgaggatt aaaaagaagt acgccgtctt caaattcgat
     2041 aattggttct tgcgaaacga tcccttctgc atcatcaatc tcaaattgtg ctttattttc
     2101 tgcaaacaag cgaacaacga tctcgatcgt atgatttttt gtatcccaat ctaaagctac
     2161 atcatagtct gtcacttgtt tttcaattat ttcatctaaa tatgttaata gatccttctt
     2221 tttcattttc tttcctccaa ccccaatctt tctcgatttg gttttttctt tgtaaagaga
     2281 tataaggatt tcccgacaca acaaaacgca acggcttttc tgtccataca cctttgtttg
     2341 ggataccgat tctaggcaaa gcattgatct tttttggtgt cttttttttc tcaaaaacta
     2401 agcgtaacga actatctgct atagactgac cataaagttc ttgaggaatt gctaatgctt
     2461 ccactaattt tcccggaccg ttgctgatat ctggaccagt ttttccgcca cgatttttac
     2521 tcatttgatc aatcattgcg gcgggttcga ttgcacgtat cattactcct tgaggaatcc
     2581 cttctggctg agtaatgatg ttcaaaatcc gatgagtatg catcgtgtaa aggtagatcg
     2641 tacctggttt ttcatacatt gcccgcacac gcggtgttct tcttaaacca tagctatggg
     2701 cggcctcgtc ttctggacct aggtatgctt ccgcgtctac aatatatcca gctaatcttc
     2761 cttctggtgt gtcatgctcc aaatacatcc ccaataaatc ccgtgccact tctggcgtcg
     2821 ttttattcca aaaaaattca tctatctttt gtttatccgt catttttctc acctacttta
     2881 tcatacacaa tattagttgg ataagctatg aaaacaaaac aacagaaggc tgttctttct
     2941 aggcatcttg ctaaatatga tataattaag ggacgtaaaa acaagaagga ggaaaatcct
     3001 atgcaacagc cattggctta tcgcatgaga cctcgaacga tcgatgaagt cgttggtcag
     3061 cagcatctag tcggggaagg aaaaatcata cgccgcatgg tcgatgctcg catgctctct
     3121 tccatgattc tatatggacc tcctggtact ggaaaaacga gtatcgcaag tgcgatcgcc
     3181 ggctctacta attatgcatt tcgcatgcta aacgccgcta cagattcaaa aaaggatcta
     3241 caagttgtag cggaagaagc aaaaatgagt ggtacagtga tcctcttatt ggatgaagta
     3301 caccgattag ataaaacaaa acaggatttt ctgcttcctc atttagaaag tggccgaatc
     3361 atcttgatcg gagcgactac tgagaatcct tatattacca tcaatccagc gatccgcagc
     3421 cgtacacaga tctttgaagt caaaccatta acagaacaag acatccagtt ggcagtcgaa
     3481 catgctttaa gggataaaga acgcggcctt ggacaacaag caatccagtt agacgaagat
     3541 gctcttcttc acctttcgcg tgcaacgaac ggcgatttgc ggagtgcatt gaatgggctg
     3601 gaactggcta cactatcgac tccttcagat aaagaaggaa ggattcatct tacactgtcg
     3661 atcattgaag aatgtgtcca aagaaaagct cttacccatg acaaaaatgg agatgctcat
     3721 tatgacgtta tttcggcttt tcaaaaatcg attcgtggaa gtgatgtgga tgctgcactt
     3781 cattatctcg cccgtttagt tgaggcaggt gatctggcaa gtatttgccg gcgtctgatg
     3841 gttatcggtt atgaagatat aggtttaggt aatcctgctg ctgcggctcg tacagtgaac
     3901 gctgtattag ctgctgaaaa attgggattg cctgaggcac gtatcccact agcagacgtc
     3961 gtcgtcgatc tttgcttgtc gccaaaatcc aattctgcct acatggcatt agatgcagca
     4021 cttgctgata ttcgagaggg caaagctgga gatgttcctg atcatttgag agatagccat
     4081 tacaaaggtg cgaaatcact caaccgtggc gtgggttatc agtatcctca tcattttgat
     4141 caggcttggg tcaatcagca atatttacca gataaactga agaatgctca gtattatcag
     4201 ccaaaagata cagggaaata tgaacaggct ctgggtcaac agtattaccg tataaaagag
     4261 tggaaaaagc atcctccaaa aaactagaca agaaaacggt tctgtttctg cgagctctct
     4321 cattgctctt tttaaattca tatgctacta tagaggtggg aaatctgtga tgttcgaatt
     4381 gtcctctttg tgtgttgacc gaacattttt attgatatct tgggacccgt cttgtgtcac
     4441 tgctggtaac atgccttatc atttggtagt tcgaagctac gaccatggga cccacctgct
     4501 tgaattcgcg ggatcaatac tacaggacaa acaacggcat taacggagat taccatcttt
     4561 taaagaataa agcaatttgc tcttggcaaa ttgctttttc tatttgttgc ttattttgga
     4621 ggatttgttc atgattagga ttttaatgat tatcgccact ttagtattat tattcgttag
     4681 ctattattta tttaataaac aggatatctt ttttgtattg atcaacaaaa atgataaaaa
     4741 tcaaggattc ctacaatttt acggagctgc atatgctgtt ttaggcgtaa tgggtatttt
     4801 aacagctttt ttcaatcaac gctttattgc cttggtcttt ttactgatcg taattattgt
     4861 ttctgccacc ttcagtatcc gatttgcaaa aaaaattgct gaaccaaagc agtaaataat
     4921 tctctttttc cctaaagtgt tctacaataa aagagtacaa tacgattgac ttgaggagtg
     4981 aatgcttatg ttacaacaat acaaaaaaat catggttgcc gtagatggtt ctgatgaagc
     5041 tgaattagct tttaaaaagg cggtcaatgt agctatccga aataacggag aacttctatt
     5101 agcacatgtc attgacacac gttctttcca aaccgtttca tcctttgatg gaatgcttgc
     5161 agaacaagct actgaaatgg caaagcaaac attggcagat tatgaaagca atgcgaagaa
     5221 agctggcttg aataatgtaa cctctgtcgt agaatatggc tcaccaaaac aaattattgc
     5281 cagagaaatc ccagaagata atcaagtaga tttgattatg ttaggagcca caggattgaa
     5341 tgctgtagaa cgtttattta ttggttctgt ttctgaatat gttattagaa acgcagcttg
     5401 cgatgtgctt gttgtccgaa ctgatttaga aaaccaattg ccagctaaag accacaccaa
     5461 agcttaacac gtagacgacc tgccaactat cggcaggttt ttttgcacaa aaaaccgtga
     5521 taaaaacaaa agacgcggca agcactcatc ggtctttata atgtttaaaa taaagggaaa
     5581 acagtcaatg agttcaagta aaataaaaaa aagtgaacac cagggcgatt agtcgccctg
     5641 gtgtggttta aaacgaccta agcatgccaa agcttattgg ttcgtcaatg tggaagtttc
     5701 cgatgaatct gatacgaaaa aatatgaagt atcgatggcg gataccgatt ttatcgccaa
     5761 actaaaattt tatttcgggt tcgggtaccg cgagaaggca agtgctacat tcggtaaatc
     5821 attcaagaat tgatgaaagg ctaccgcttg ccattgcaac cacaacgata ctcctgaacg
     5881 cgatgttggt gagatttcca aagagaagag ctgtttaatg caacggtatt aagcaaatgg
     5941 caaaaggaag tcatgtagac aaagtttttt atcaaacacc atatgatttc gcctgaacgc
     6001 tggtttagct cagaatacaa tgatgtggtc cacgagacat taccctcagt cagataaaaa
     6061 aagctctctg acagaattac acatcgataa atattaagta aatgtcagcg ctagttgaac
     6121 tttctgacac tttcaggtac actggaaaag acaactacac gaaggaggga aaatactatg
     6181 atgcgcatga ctaaaataac aagtaatgaa aattaccaac gataccatat cgcatttagc
     6241 catgcaattg gttttacagt tgctgttgac gggacaagtt tgtccaaaac agttgaattc
     6301 acgtaaaatc aatggccatc tcaaggtatt tttctcatga taatctgagt ttaaataaca
     6361 caggccatgg ataaccatgg cctttttgag tggtctcaag gagagagatc atgatcatac
     6421 aagcaatttc tattaaaaaa acaatcggcg ggaatccatt atttgaggat ttatcctgtc
     6481 aaattgaagc tggtgaaaaa attgccttag tcggtatcaa cggaactggc aaatcaacct
     6541 ttttacaaat cttgaccggc agagaaggtg tcgacagcgg tgttatcagt cgcaaaaaag
     6601 gcttaaagat cgggacggtc gaacaagaac ttacagtttc cgaagcgacc gtcaaccact
     6661 acttgctcca ttctgccgct gagatacagg acctcaaaca gcaaatgagt cgctacgaag
     6721 cattgatgac agaaccagat acaaacttag agaagtgtct ggcccgttac ggtgagctgc
     6781 agcatcgctt tgaagaacta ggcggttacg tattagaaga ccggattgca gcgatcttgc
     6841 aaggcttagg gattccacac cgtcaggaag cacggttaac agaacttagc ggaggcgaac
     6901 gagttaaagt cgctttagcc aaaattttag cagccgatgc cgatttactt ttactcgatg
     6961 aaccgaccaa tcatttagat cttaacagta ttcgctggtt ggaaaactat ttacagaata
     7021 caaaaaaatc ggttctggtc gtttctcatg atcggcaatt tctcgatcaa gtcaccacaa
     7081 aaacatggga actggaagaa ggtgcattga ttgagtatcc cggaaactac agccgttttc
     7141 gagtcttgaa agaagctcgc ttggctgagc tgacaaaaaa ttacgaactg cagcaaaaag
     7201 aagtccagcg gttgaaagtc atgattcgtc gttttcgtca gtgggcacat gaaggagaca
     7261 atgagtcctt cttcaaaaaa gccaaagaaa tggaacgccg cctggcgaaa ctgacactcg
     7321 tcaaaccgcc acctcccccg aaaaatcggc tgcaaagctt gtctaatggt ggaaaatctg
     7381 gcaaagaagt tttcatcatt caaaacttgc accagcagta cgctgatcaa gtactattca
     7441 aagacagcag ttttgcagtc tatcgtggag atcatttagc catcatcggc gacaatggtg
     7501 caggaaaatc aaccttactc aaattattac tcggtgagga aaagccttac ggtggcacca
     7561 tcaaattagg tagtagcttg caaatcggtt atttgccgca gcagctgcag tttgccgatc
     7621 ccgatagccg tttgctggct tacgccatca cattgactgg aaacgaagaa gtcgctcgac
     7681 ggcaattagc caaaagtggc ttttatcaaa gcgatgtggg taaacggatc aaagatttat
     7741 ccggtggtga aaaaatccgt ttggcgttgc tgaaactctt tttagaaaaa atcaacgtcc
     7801 tgattttgga tgaaccaacc aatcacctcg acagctatgc tcgagaagaa attgaagaaa
     7861 tgctgcaaga ctatcaagga acgctcctag cagtcagcca tgaccgctac tttttgcagc
     7921 aacacttcca agaagcactc gttattgatc aggagcagat cagccgccag ccgctcggcc
     7981 aactgattga ctaggacgtt ttttcgcaac aaaaaacttg attttcatac ctgtgctttc
     8041 acgattcagg caaagtacag gtggaaaatc aagtttttat tattcgatca atgaatgttt
     8101 cggatcggtt ttctagctaa acataaagcg aagaataaag tcgacagccc acgaggctga
     8161 tctaaaaatg ttttttcttt ttacatatgt aatgtccctt ataaagatga atgaaaataa
     8221 aatattggga ttttcctgaa aatcaataaa taaaatgcca gtcttttccc ttttgaccac
     8281 ttttccaagc aaaattccct aagaagatcc tgtaaaaaag aagggaaatt gaagttaaat
     8341 taccattttg cggtcttctc cgcaaaaaaa acacatgcaa gaattccctc ttgcatgcgt
     8401 ttcatctttt atcctaacga atgctttggc ttagccgcgc agacgctcca catcacgggc
     8461 aatcattagt tcctcatctg ttgggatcac taatactctt acttttgatt cttttgtaga
     8521 gatatctact tcttcaccac gcaattcatt caacgctgga tcgatttcac aaccaaacca
     8581 agacatacct ttgatgattt cactacgtac atgtccgtca ttctcgccga taccagcagt
     8641 aaagacaatt gcatcaacac cattcatcgt tgtcacataa ccgccgatgt attttcggat
     8701 gcggtccacg aaaatgtcat aagctaaacg aatatgttct ttttcatagt tcttttcaag
     8761 atcacgcata tcactagaca atcctgttag gcctagcaga ccagatttct tgttcaaaac
     8821 attgatcatt tcatcaatat caatctctaa tttttccatt aaatatggga gaacagctgg
     8881 atcgatatca cctgaacgtg tacccattgt caccccagct aatggtgtga atcccataga
     8941 agtatccatg gatttacctc catcgactgc tgtgatcgat acaccatttc ctaagtggca
     9001 agtgatgatt tttagttctt cgatcggttt gttcaataat tttgccgcac gttccgacac
     9061 ataacgatga cttgtgccat ggaagccata ttttcttact ttgaaatctt cataataaga
     9121 caccggtaag ctatataaat aattttgctt tggcattgtt gagtggaaag atgtatcgaa
     9181 tacagccaca ctcaaaatgt caggtaaaat ctttttaaac gctttgatcc ccattaagtt
     9241 tgcaggatta tgcaaaggag caaattctgc taattcagca atgcggtcca ttactttctc
     9301 atcgatcact actgaatccc cataagtttc tcctccgtga acaacacggt gccctactcc
     9361 ggtgatttca tcataagagc ttaaaatttg cagttcaatc aactgatcta acagcatttt
     9421 tactgctact tcgtggtttt cgatatccat gatttgttcg aatttttgat tatcgccgta
     9481 cttgatggta aaaatagaat cttgtaaacc gatacgttcg acgatccctt tcgcaactac
     9541 ggtttcttct ggcatttgat ataattgcca tttcaaactt gaactcccag cattgattgc
     9601 aattgtttta gacatatgac tctccttctc gttgaatgat tacagagcag atgatttcca
     9661 gtttttaaac tcgttgaaaa attccgtcac cttctctggt tcttttaatg aagcaagttt
     9721 cacaagcaag acttctttta cttgttgtgc ttgtggtcct tttttctgta agatcaaaat
     9781 acttttttgc gattgtttat tacggaacaa ttcatctggc aattgaatca ttccttgaag
     9841 ataaccttct tctttaaacc attttttgat ttcttcactt tgatccgttt ctaaaaatcc
     9901 actcggcatc aaaaacaaac caaaaccatc cggcttcaca tatttcattg attgctctag
     9961 caataaatga tgtgcataac tatggccttc ttcggtactt gttaaaaact cttttgcttt
    10021 ttcgtcattc ggataataac cgattggcaa atcgctgatt gcaaaatcga caggatctat
    10081 taataattcc tgcaacccgt cttgatgaaa gtattgaaca tttgcttgtg tcaaatcact
    10141 tgtagaagct gcgacagcca acaatgtatc atcaatatcc acaccaattc cttctgtttg
    10201 gtaccccgca ttcgataggt ttaaaagaac tgttaataac aagttgccca ttcctgccgc
    10261 aatatccaga atcttaaccg gtgctttttt atttgtgtat agttgttcaa taagaaaaac
    10321 aaataaaaaa ccgatactat caggagtgag ttgatggttc gcctgcaaat gttctgtctg
    10381 gctgcccttc aataaaagta attgagacag tctgcgccat tcttctggct cgaaagaaag
    10441 tttcttcaac tcttcataaa gagctgtgat ccgttgcgtt gtctcttttg tcggcactcc
    10501 atccaccaca cgtacttgat aatcatctat taaattttct gcattttcga cataggcttc
    10561 taaaaaggaa gttcccaatg cattctgtaa aagctgaata gcttccaggt tttgattaaa
    10621 cccttgttcc attttttctg gtaacatggt gcacctcatt tctagatata ttataacaat
    10681 tttttcacct gtttaattct atcgaacaat tgacaaaatt tcaatcgttt tcactgtttt
    10741 ttatttgtga aacggcaaac atttttttat taaaaacagt gttataacag aaaagagggc
    10801 gttttcacga acattccatc attctgtact agtatctgta tcatttttta cttcttctgt
    10861 gaaccgaaag ataaacggtg agacttccac ctctaagaat agggactgtt gtacccactg
    10921 atatgttaca gatccagttg tgtagtaaaa aactccttcc gaactttcgg caagcgtaga
    10981 ctgttccgat aaagccatct gcttcattat ttttgctata taataatttt tgattcgtat
    11041 attaagatcc attgacaaac ggtagctttc taatgctgcc atcaataaaa acgtacataa
    11101 aagaaataaa agcaagacag aaaataaaat agagccctta taacattttc tactgatcgt
    11161 tttttgtcca tatggcatat ccatcttctc cattttcaaa gtgtacataa aaagaaattg
    11221 tgtgttcttt ttccaataat tgaagctcag tgatacttgt taacataggt tgatgtccgc
    11281 cggaagttct tcttttacgt atcattgcct gatatctttc gatgatatac tgtttatcct
    11341 cgatcgtgta gtgcagttgc tgatttttca cagagacatt gtgactgctc ttgagttctt
    11401 cttctaattg tgctaaaaat attagccact ctttttgatc tttacgctgc aaaaattcac
    11461 ttacttggat ggtttgctga atcaaaagat tgaatgtgag caatagactg ctcaatatca
    11521 tcagtccaat tagacattca atcagcgtaa atgctggaat tacttgtttt ttcaatactg
    11581 atcgcttctt tctccatatt gacaaacact ttttcgatta ctccattatt atcgacaatt
    11641 tggatatcgt atgggatcat ttcttccctt cgctctggtc ttttgtgata catccgctgc
    11701 gtcttgatat cttcataaat cattcgaata aatagcagtt cctccccact tttcttttct
    11761 gcctgaagca aaaacagctg gccaaagctc actaagtaga cacagacaga agcgatcata
    11821 agcgctgtaa gggactccaa caaaacaaat cctgttttat tcaattcttt ttgtatagcg
    11881 accactccct atctggaatt gatacgtaat cgtccgctct ttttcttccc aataaaattg
    11941 ataagctttt agtgaacttt cattcccggt tctagcagca aaagtaatag aagcatggcg
    12001 tttcaaggta atctcatcag gaatatctaa tacttcccaa tttattttat ctggattcgg
    12061 aacatgaaaa atgatttgat tctcttcact attccaataa aagcctgttt ggatctgatt
    12121 cacaatagcg attttttgcg tagaatagat tcgtttttca aattggccga aaaattgata
    12181 gactgccaat tcattcttcc atgcagaaaa tgcgagaact ggaaaagaaa ggatcgttgt
    12241 aagtaataaa atcagtaaga gtgattcaaa caacgtatat cctgatgcta aaaccttacc
    12301 ttcaaggccg ctgatagatt tctgcatgtt ctcgtttgat atagccttca ttcaacaatt
    12361 cctccacagt aggaactctt ccattatttt gaagcgcata gagctctttt tgtgtgtcta
    12421 ccaactggat gatcgcttca cgactttctt ggtctacatg attcctataa cgcgacaaat
    12481 tagggacgaa caacaacact aatacactga tgatcagtaa gacgattagc atttcgatca
    12541 atgtaaaacc gctataataa gatatttttc tctttttcat ggcaaaacct cctctatgct
    12601 tccgtaaaca ggtaataaaa cagcggcata aactaataaa atcaaagccg ctacgcccaa
    12661 aaaaatgatt ggctgaataa aatgcatcca gtgatgaaga cgtttgaaaa gctgttttct
    12721 tgtccattcg ctgtaaaaaa tcagttcttt acctaaactt ccctttgcct ctccctgtaa
    12781 aacaatttta ctgaattctt ctgatagaaa acgataattc tggaactgct cagccaatga
    12841 catacctgcc tccaatcctt gaatcaactt agaggcaaga tgctgaatca gactttcttg
    12901 tttcgtttcc tttaagcaga ctatgatttg ccttaattcg aaaccttcat gaaaaagctt
    12961 tccaatctct agtgacaaat aagccgattg atacaaggaa tatatcggtc caatcaataa
    13021 ttgtctgctt atccattcac tctgatcaac aggagacaac ttcatcagac gataccttac
    13081 tagtaaagcc aatagagtaa ccgctaggat gaatagtaga acataccaat gaaagacttg
    13141 tatcagctga ataccccaat gctcttgcat gaccattcct gaaccaataa gctgaggcaa
    13201 aacgaacaac cgcatactaa acaagatacc tagcaaaaag gtgagcaata gcatcggata
    13261 tgcaagcagc tttttcaaat cattgcgaaa tccttcaaca agccgaaact gtttggcaat
    13321 ttccaacaaa gttgtcggca gattcccatg acgttcagct aactcgatct gtaccagttg
    13381 ctctgctcca tatcctattt gcgaaagtac ctcactaaac gcttgccctt cctttagctg
    13441 atgttggata tttgccaaaa taacaggtgg aaacgaacgg atcgtgagta ataaataaag
    13501 ggattcttgc agactaaaac cagataaaag cagttctccc atggtttgtg caaactggtt
    13561 ttgctggctt cttgttaatc ctttagaata gttctgactg ccaaatattt tcctgagctt
    13621 cttcataact ttcctcccat gattttttta ctgattcttc ctccataaat tgataactcc
    13681 ataaaacaga aggatggtga ttggcatctt ccaaaagttc ttgataaatg acaccagcta
    13741 aacattcaga tagctcttct tttttctttg tcagttcatg gattctgacc aatgtacctt
    13801 caagattgcg tgcatgtacg gttgcaaaaa cccggtgccc tgttagtgct gctcttaccg
    13861 cagcatgtgc agttgattca tctcttatct cgccaatgat tagcaagtct ggacgatgcc
    13921 gtaatgcgag tctcaaaagt gtatcataag tctgtgctat tttcggattg atctgtagct
    13981 gcagaaaaaa cggttcttct atctctacag gatcttcgat agtaatcact tgcaattttt
    14041 cttccaaagc caaccgatac attagagaag tcttgcctga tccgactggt ccgctgaaaa
    14101 ggtataatcc acgtctgacg gttttctgta aaatagttgc aaaatctcga gaactaaagc
    14161 aacgatgggg tgtttcaaaa gtaccgtata atagccgaat cactaaactc tctcgttgaa
    14221 ggtaatctcc tacagaagac aaccgcagac gaacttcctg tttttctaaa gaataagtaa
    14281 atgcacctaa ctgagctttt cttttttctc cgatatccat ctgtcccaaa tacttaaatc
    14341 ttgagatcag ttgctgtcca tctgccgaag agactttctt ccagaatgtt cgtttttttc
    14401 catagcgaaa atagatgtaa aatacttctg aagctggcaa tatatatacg tcttgggcat
    14461 gtttggcaaa actttcactt atcatttgat ccgataatcg ttttatttcc attattctcc
    14521 cctccacaaa aaacatacgt ctttttgatc tttttgtttc aaataaaatg aatttacaaa
    14581 aaaatattgg caaaaaccga atttatgtta tacttataaa aatttatctt gggaggcact
    14641 ccattggaaa caacattaag gaaatatatc gaagtagcaa gtaaaaaaaa gcctgctgac
    14701 ctagtgatca agcatgctaa gatcgtcaac gtctttacga aagaaatcat ggaaaaagat
    14761 gtagcaatct gcgaaggaat gatcgtagga atcggtgaat acgaaggaaa aagcgtacat
    14821 aatgctaatg ggcaatatct agtgccagga ttcatcgatg gccatgtgca tatcgaaagc
    14881 tcacttttat caccaagcga attttcaaaa gtatccttgc ttcatggagt tactactgta
    14941 gtgacagatc cccatgaaat aggaaacatt gctgggagta caggtcttga gtttatgata
    15001 gaagatgcaa gacaaacact catgaatatc ttttttatgc taccttcttg tgtacctgta
    15061 accccttttg agaccaatgg tgcaaattta aatgcaaaaa aattagcccc ttttttacaa
    15121 aaacccgaag ttttaggctt agctgaagtc atgaactatc aagcagtagc aaataatgaa
    15181 acagacatcc tagaaaagat ccaattgatg caccagcata agaaaaaaat tgacgggcat
    15241 gcagctggta tcggcatgga agagttaaat gtttatcctg cagcaggtat tcggacggat
    15301 catgaagcta caactgcaaa agaagctaaa gaacgtttgg atcttggtat gtatttaatg
    15361 gttcgagaag ggaccgttgc aaaagatctg gcatcgctac taccggcgat cacacctgaa
    15421 aattcaagaa gatgcttctt tgtcaccgat gataaactga tcaatgattt agtaaaagaa
    15481 ggatcaatcg atcatatcgt ccgtcaagct attgcacttg gaatcgatcc attacaagcc
    15541 attcagatgg caacattgaa tgcagcagaa tgttttgatt tgaaaacact aggtgcgatt
    15601 gcccctgggt atcaagctga tttcttctta acagacgatc ttaccaagct gccaattcat
    15661 gaagttttct ataagggaac attagttgtt caatctgggc aactccaaga ccatttattt
    15721 gctgatcatt caaattcata tacgtgttgt ctgccaaaac taaacgctca accattaaat
    15781 ccacattcgt tagagctgct tctttcttct tcacaagccc atgtgatcga aattatcccg
    15841 aatagtctag tgactaaaga tttggtagaa aaagtggacc gacaaggcga acgattcatt
    15901 ccttcaatcg aaaaagacca attaaagatt gcggtgatcg agcgtcacca tcaaacggga
    15961 aacattggat taggcatagt gaaaggattt cagctgaaac aaggagcgat agcgacaacg
    16021 attgcccatg actcgcataa tctagttgtc gttgggacaa acgacgagga tatgctgtat
    16081 gctgctaatc ttttgattca aaaaggtggc gggatgaccg ttgtcaacaa acagcaggaa
    16141 atagcttgtg tttctcttcc tataggcgga atcatgacac aggaaccttt ttcgatcgtc
    16201 tatgaacagt tgaatgaatt aataactaaa gcacatcaac taggagcagc taaaaccttt
    16261 gatccttttt tgacactttc tttcttaaca ttatctgtca tacccgaatt gaagatcacc
    16321 gacaaaggat tattttcatt ttctcgcttc caattgattc caccttgtgg aaaaaccgat
    16381 gaaataaata cattttaaat caaataaatc ccagaaaata cattacatct gtaatgttca
    16441 ttttctggga tttatttatg gatattaatg tttttatgaa agcagaaagt tagtttcaca
    16501 gacttttctt tttggtctta tcttgaaatt gagcttctac tcgataaata cgttgacctt
    16561 ttgaagaaaa tttttcttca tactccgtca taatattgcc ttcaaattga ctttgatgta
    16621 aatctaacca tacctgtttg aggatcatgc catattgaga aaaacttgcc agagaatact
    16681 caaacaatcc ttgattatct gttttgaaat ggatttctcc atttggacga agaatctgtt
    16741 catctacagc aagaaaagtc ttatatgtca atcgccgttt ttcatgacgt ttttttggcc
    16801 atggatcgga aaaattcaga tagatttgat ctacttcgct gtcagcgaaa tactctgtca
    16861 acgctgatcc atccacatgt agaagtttca ggttaggaag cggttgttcg atcaacttgt
    16921 ctagtgcaat cgaaacaaca ctgacctgca tttctactcc aatataattg atttcaggat
    16981 gggcttttgc cattccagtg atgaactggc ctttccccat accaatctca atgtggattg
    17041 gatgatcgtt ttcaaacaat tcattccatt ttcccttcca caatgttgga tcactaatta
    17101 caaaattcgg gtgagccgct aacatttccg ctgctcccgg acgatttcgg acacgcatta
    17161 tttatctcca ttcctttcct ctaattaaaa aggtctggcc aaatctccgc agagtttggg
    17221 ccaaacctca gccactgaaa attcgtttta attgtaaaat ttcttcgttc atccgtcgat
    17281 gatcttcacg tttgcaatat ctggtgattt cttggagcaa attcatggcc gcataccaat
    17341 gaaatttttc aagcatttcg tcagtcgggc gtatgccata actgatcagc cactgattcc
    17401 agctagacag tggcacataa tttcctaaaa tcgttgcaat atccaccgca gggtctgcaa
    17461 acatgattga atcccaatcg actagatata aataattttt acatactaac caattacgat
    17521 ggtttacatc tccgtgtact actgataagt tttccttatc tattgacggg atctgctcac
    17581 gaagaaaacg atggacaatt tgcagaaatt gatttttctg gagttcttcc ggtaattctt
    17641 tttcatattt ttgcagcatc ttctccggcg tcatgacacg accaccgata cgggtcagca
    17701 tggttttaag agaatttgaa tgatgtaaat ggtacaaaac atcaatcacg tcgtttcttt
    17761 gaccaatctc attaggagtc agcaagcgac cttccagcca ctcttgtgct gtcaaagtat
    17821 cgccattccc cgttcgtttt gtccaaacaa gttttggtgt aattccttcc cttgaaagag
    17881 cagccaacat cggagtcgtg tttcgtttaa taaagacttt gtcctcatca cgcatgccta
    17941 tgtatgtttt tcctgtgtcg cctttgatgg gacgtaaccg ccactcttgg tctaactgaa
    18001 actccatcat gcattctccc gtcctcttac tctttgtttt gcagtactta ttattctagt
    18061 cgcctgaaca ctacacgtca agcacatata cgttcttttt catttaattg ccattcaaaa
    18121 aaccagcttt tctcaaaaaa aatagaacga acagcattct tactgtcaac gctctatttt
    18181 tcattctcat ttttacccta cacagctttg ttttcagtct gacatcttct tcaaacgagt
    18241 tggtacatat agtttaacaa agagcatagt aacaatcaaa taagccgcta gcggaaccca
    18301 tcgctgctgc ccatctaata cgactgcagc aatcaaacca aaaataaccg acgcgataat
    18361 taatacccat gtgagtagat actgaatcgc ctgcttcttc tgattcttat ggattggata
    18421 aagctgaacg agaaccatat agcggaactg gttcgctaaa ggtaacagtt ggaaaccgat
    18481 caaatataaa aataagaaac cgataatcag actaaaataa aagtcgacta ctccggcaat
    18541 caataaccca cctataatta ctaatcggaa ataaagacca ctaaaatcag aacctcttgc
    18601 caatcttctt gcaaacaagt aaagataagt gttttttgat tctcttttaa tatgagtcaa
    18661 tataggatcc aaatagcgtc ttcttttgat ctttgccgta atttcaggaa catctgtgaa
    18721 taaattgata aattgataga tctgatgcaa cctatgttct tctacatgca ccattttctc
    18781 ccaatccaat cctttttctt gttgcttcca caaaaaccag taatatagac ttgcctgtac
    18841 taatgcacct gctattcctg cccatattag ccaataaaat gatagaaaca aaaagatcag
    18901 agaagaaaaa aaccagccta atttccaata acgaataact tgttgatcta gtcgaaacaa
    18961 ttctgctctc ttgatttgca gatgactgaa tttcaaactc cataatatga tcagatagac
    19021 gaaaaacatc gaaaaggatt gctccgtact aaccaccact aatggcatgg ccaatccgca
    19081 tgccaatatc aaaaagatca acggaaaaat gcaagaatag cgaaaagctc gttccaaata
    19141 ggtttgcatt tctttttctt taggaagtaa aaaaacaata tctgctgcct ctgcaaaagt
    19201 agccatctta cccacgaata aacagagcat ccaaaataca gagattatga gccctccaat
    19261 tgcaaacggt ggctgcaggg ttttcaacca attagaataa tataatccca caccgccaat
    19321 caagaaaaga caaactaaaa caaaatgatc gttcaataca taacgcatat atttcatcat
    19381 tttcttttga tgtcttgcta aacgtttttg aaaaaaacta ctcatgtgcc tgctcctttg
    19441 ttaaagcaat atagatttcg tccaaagaag aatccggcag ctgaaattct tttctcaaca
    19501 tctcaagaga accgtctgct cgtatctctc cgttatgtaa gaccacaaaa cgatcacaat
    19561 atttttctgc agtagctaaa atatgcgtag acatcaaaat accggctcct tcttttttca
    19621 tttctaccat caattcaagc aatgcattga tagctagtgg atccaagcct aaaaatggtt
    19681 cgtcgatgat atataaagaa ggctcgatca aaaaggcgca taagatcatc actttctgtt
    19741 tcattccctt tgaaaaatta gctgggaacc agtcaagacg attagataac cgaaaagtct
    19801 ccaacaactt ttctgctctt ttcattgcta tatccatagg tatatcgtag gccatcgcag
    19861 tgacttcgat atgttctctc aatgtcaatt cttcatataa agaaggagtc tctggaatat
    19921 acccaatttg tttgcgatat tcttccgtat ttttctttaa ggatagatga ttgatttcta
    19981 tcgttccttt tgaagcttcc agcagaccga taatgtgctt gatggtcgta cttttccctg
    20041 ctccgttcag tccaatcaac cctactatct ctccaggctg tacatcgaaa ctgatatctt
    20101 ttaacacagg gagatgtccg tacccccctg tgacatgatc gataattaaa ctcattcttt
    20161 ttcctccgcc atttattttt atttattata ccacgaagaa aatgacggta ttttgttttt
    20221 ttccttacaa tagctttaac ttatggtaaa gttaagctga agtatttatt ctttatagaa
    20281 aggatgtttt atatggaaaa ctgtattttt tgcaaaatca tcaatcaaga aatcccaagc
    20341 tataaaatat atgaagacga caaggtctat gcttttttag acatctctca agcaacgaaa
    20401 ggacacacat tagttgtgcc taaacgtcat gtagcagata tttttgaata tgatcccgaa
    20461 cttgcaggag aggttttttc gcgtgtgcct aagattgctc aagcattgga aaaagctttt
    20521 ccagaaatga aaggtcttaa tatcttgaat aacaaccgtg aactagccta tcaatctgtt
    20581 tttcattcac atatccattt agtccctcgc tacagcaaag aggatgattt ttctattcac
    20641 tttgtcaatc atcaagatag ttatggatcg gaagaactaa aagctattca agaaacaatc
    20701 gtaaagcagg tgtcctgcga tgattaaaaa ctttttcaaa ggactgttat ttggagctgc
    20761 tgctggcact gtcggaggtc tgttattggc gcccaggagc gggaatgaaa caagaaagaa
    20821 actgatcgaa gaattggaag aagcaacaac tctgacaaat gatttaaata acagcctgaa
    20881 tcattttaaa agcgcattga tcgaaacaaa agaaacagct gaatctgtga ttcctgcttt
    20941 ccaagaagca atcaaaaaag atatcgaaga ttttaaattc caagccgaac cacgtatcac
    21001 acaaatcaat gaacaagtag aaaaactaaa agctgatctg ccagagtatc ctgaacaaat
    21061 ataataatga ttattttctc aacagattta actatataag tgagaaaagt ttgcgtattt
    21121 cctaggaatg tgatgaaatt ttgaattttg tttgaaacta ccacgacaac gtttttaaag
    21181 gaaatgggat atggtatagt taatatataa aaataataac taattacagg agtgcaaatt
    21241 ttaatgaaaa aaaaatcaat tattttggca gctactagtg cgttggctgt tttaactcta
    21301 gcagcttgtt caggcgatac taataaagat atcgcaacga tgaaaggcgg aacgatcact
    21361 gtatctgatt tctatgatga agcaaaactt gaatcatcga accaatcctt agtacaacgg
    21421 atgatcattt ataaagtatt caataacaaa tatggcgata aagtaaccga taagcaagtg
    21481 gatgcagaat acgacaaaca agcgaaatca ttaggtgata cttttgaatc acaattagaa
    21541 gctgctggat atacgaaaga tacttataaa gaatatattc gtaacaattt agcttttgaa
    21601 gctggcttga aagctcacgt agatattaca gatgacgatt tgaaaactgc ttggaaatct
    21661 ttccatccag aagtagaagc acaaatcatc aaattatcaa gtgaagatga agcaaaagat
    21721 gtgaaaaaat cagcggatga tggagatgat ttctctaaat tagccaaaga caaatcaact
    21781 gatacagaga caaaagaaga tggcggtaaa gttaaattcg actcaacgac aacaactatt
    21841 cctgcagaag taaaagaagc agctttcaaa ttgaaagacg gtgagatctc cgatgttatt
    21901 actacaacaa atcctacttc ttatgcgaca gaatactacg ttgtcaaaat ggtgaaaaac
    21961 caaaataaag gcaatgacat ggataaatac aaagatcaac taaaagatat cgctactgaa
    22021 actaaactaa gtgacaatgc attcacaact aaagtaatcg gtgaagaatt aaaagatgca
    22081 aatgtaaaaa tcaaagatga tgcctttgaa aatgtgttga gtgcattcac aacaacttca
    22141 tcaagtacga aagactcaag tgaaactaca gcttcaacaa agagttctga cacaaaatca
    22201 actgattcaa caaaagaatc ttcaactgaa gaaacaactg attcatcaaa ataaaagaaa
    22261 acaaaaaaag cgaagactta tgtctcgctt tttttgtttt gaatgaaaaa aataaatttt
    22321 tgctataaat cttttggtac atagaaactt cggttgtcca taccgaaaat ccgatcggtg
    22381 tattttcctg gttctgtttg acgaatcgaa gtcaacatca tctgcatcga agcatcgata
    22441 ttgtcgattt gatgtaatat ttctgcttcc attattcttg gacgtacagg tgagccatat
    22501 tctaataagc catggtgtgc taaaaccata tgacgtaata cgagaacatc ttcgtcagct
    22561 tcgctgattt tcaacgcaag acaggcttta gttatttctt cgtcaatcaa taccagatga
    22621 ccgatcaaat ttcctgcaag cgtatattct gtcgacatag gaccggaaag ttcaatcacc
    22681 tttcctaaat catgcaaaat gataccagca tacaacaatg ctcgattcag ctcaggatat
    22741 tcatcggcga ttgatttccc taagtgaagc attgtcaaag tatgataagc taatccactg
    22801 gcaaatgcat gatggtttct ttttgctgcc ggaaactcaa aaaattgttt ttgatattta
    22861 ttaagtagat agcgaacaat acgattccaa tgcgcattgg tgatctcaaa caacaattga
    22921 ttgatttctt cttccatttc ttctttcttc aaaggggctc tttccatgta taaactaggc
    22981 tctcctggct catctaccct tgttaagcgc atatgaagaa tctttacttg agggtttcct
    23041 tgataaacct ctcttttacc gtttagaaaa acgactgtgc cggctgtaaa tttattgatt
    23101 tcctcctccg aagcatccca gtatttacca tcgatagtac ctgaagtatc ttggaaagta
    23161 aaagcaataa atttttttcc gtttttagcg attcttacat ctgcactttt gatcaggaca
    23221 aattgttcaa actgctcatc gattactaaa tcacgaattt ttttcattat ttgctccttc
    23281 taattgtttt acgatttggt tcatctcatg gtaatagctt accatttctt tatcagaaga
    23341 aaaacaaatt atttgatact tttctccaaa tttagctaaa agttcagcta attgatactt
    23401 tcgctgatga tcataatgaa gccagccatc atcaatgatg atcgggcata tcgaacggtt
    23461 agcttctaat gagaggtaag caaatctcat cgccataatc agctgatctt tcgtacctgt
    23521 agataactca tagatgggaa attctgtata tccgtctgtt aaagtcagtg tatcattttt
    23581 cagtaatatc ttctgataat ttccttttgt taaaatacct aaataatcac ctgctgctct
    23641 taacaactgg ggtaattgtc tttcactcat ctctgttgaa agatcaccta agaatgtccc
    23701 ggcgatttca agtgctcccc attcctccag taattcctgt aattttgctt tttgccggct
    23761 agcttgttga taaagttcgt ccaatgtgcc gtctctttgc aactcttcta cttgaagttg
    23821 gagtcgttgc cgattttcac ttgcttcttt ttcttcttgc tggagttggg attgcttttc
    23881 tttcagttgg cgcagtctta cagataattt ctcttttgtc agctcttcag gaaatagtgg
    23941 agtaagcatt tccgataatt cattgtatct tttcatctgc tgttcggctt gtttggcttt
    24001 ttgtaaaaag acaggtactt ccgaaggata agataagcct gcttgagtca attgttcgct
    24061 gtattcttgt atccatactt tttgtttctt tttcaattga ttgatttctt gcttcaataa
    24121 agtattttgc tgatagcttc tggcaaattt catctgttcc atctcttgta taaaacggga
    24181 aagcaatacc attttttctg aaattgtctt atcttgtatc ggtaaccatt ctttaacaaa
    24241 ctcaaatttt tggtcgattt gtcgtagttt ttcttgtatt tcctgttctc ttttatgttt
    24301 ccgctcaaat acgccatata atttctgata atctatgact tcctgtccat attctttcat
    24361 gagtttgagc gtatccatct ttcctaagtg ccgatgatcc gcaaaacgat atacttcttg
    24421 ctgaagaatc tgtttttctt ctccagttct cttttgtttt tcttctgctt cagctaattg
    24481 agctaaggct agatcaagtt gtcctagttt ttcttgccac attcctttta tctcagagaa
    24541 atcattttta gttttaattt ttggttgcct tttgtagaag aaatagccaa tcatgcctgc
    24601 accaattagt aaaaggatca ctttcaaggg cattggtaca aatatacctg caatgattat
    24661 tgctccgcca gccatcagca tatacggttt agatgaagag actggtgcta tctctgactg
    24721 attttgtaat aatgctggat aggtctcttc caagtcattc acttctgctt caatcatctc
    24781 ttgctgttct ttagctattt ggagacgcac agaaatctgg ctttctagat cagcaatcct
    24841 tctttcttca gccagaagat gcttccattc ttcttcatat aaaaattctt ccggtaacgg
    24901 atttttctgc caatgccacc tttgttccaa aatcttcaat tctgaatttg tttctctagt
    24961 ctcaatttgt aatctttgcc attcttcgtt taattgttcc gctgtaaatt gaagttgctg
    25021 gagatctttt agttcctgct cctgctctaa ataaaaataa tatctggctg actgctgatc
    25081 catcccgcta tgcttttcca gtgttgcttc taattcttcc aaacgctcat ttgtttgctg
    25141 ataccgtttg gagaaagact ccagatctaa aagaagttgc tcatcaggaa gacctgtagt
    25201 gctgtttttt ttgagagatt gccattcttc atataaagaa aaattaagtt cttgctgtct
    25261 ggctaatgaa aattgtttgt tcgcctcttt taattgtgcc aataactgct gctgttgcga
    25321 ctcgtattcc tgttcttgtt ggatcaactt gccaaaataa gcttcctgtt cttgtttttg
    25381 atgaattttt tcttgcagct ctttccattc atttaatttt tgattgatta gtaatttttg
    25441 gccttttttc ttgaataatt gttgtgcttt ctgataatat tcttgccgct tttggaatac
    25501 ttgagaactg cccgtgatgc ctaaagaaat cagcgcatca tgtagctctt tttctctcaa
    25561 atgatctagt tccatcaact gctcttgctg gaaagtaaat acctgctgga ataactcttt
    25621 cgtcaaaggt gcgatcagtt tttctagtaa ctcatcactt cccacttgat cccccagcca
    25681 caccttcgat tttccgcgat tttgctgctt gtagcgctcg acagcaaatt caccatatac
    25741 aggatgttta agccatattt tgccgccata ggctgagccg tcttttggtg tataatcctt
    25801 ttttttacgg ccttttgaag ggaaaccaaa taatattgct tgaataaatt gataaatcgt
    25861 tgattttcct gcttcgtttc ccccatagat cagctgattg cctgaataaa agtcaaaact
    25921 tttttgacgc cattttccaa aagcagcaat ctcgatcctt aaaatcttca ttcctgatcc
    25981 tcctcaaata aaaaatcttg attaagcagc tgcttggctt tttcaagtgt ttcttcttga
    26041 tattccggca gttcgctcaa tattctagcc gcttcttgat gactatatac ttcatttaaa
    26101 atttgttgga aatcttgagg tgtctgatag tattgaaata actgttccat caatttagca
    26161 gaagcagcaa gtggggtctt cgtataaaaa gtattctcta tgagagaaat acgccaaata
    26221 aagaaatcat ttatcctatc tgagaattcc tctaacaaat agtctaatag ctctcctgat
    26281 tgtatccgtt ccaaaacatc tgtattgaga tggtcataat cttttaattt tatctcaatt
    26341 aaactaaatc catcctctac ttttgaaagc tgctgacgaa tgtgaacaat gacctctttt
    26401 gtcgttcttg ctttcctcaa tgatatttcc tttgtcttcc aatagacctc tgctacttta
    26461 atcggatgta tttggcaact tcctttactt aattcaacta gtagaataga tgtagaagct
    26521 tgttcctttt tagtatgacc ttgaggcgca cctggataaa caattgtttg cttttcattt
    26581 aagaccgttg gcacatggat atgccctaaa gcccaataat cgtatccctt ttcttgcatc
    26641 tttgagggtt ggaaaggagc atagtttcct ttttgagaga cgccaggttc cccgtggtac
    26701 aacccaatat ggtaatctgt gagttctcta gaaggaaatt ccatcacttt gtctttttga
    26761 atccacggtt gacgataact aaatccactg agagagacag tttctccact ttttatcgtt
    26821 attttttttg tctctaccgt ttcggatgta aataaatgga catttttagg aaaggagaac
    26881 caataccgct ctttttgata gaaatcatga tttccgaata ttataaatac aggtatatgg
    26941 ttcttttcta accgtttcat ctgttctgaa aaatgtttct gtatttttaa tgaaggacga
    27001 ttttgatgaa aattatctcc tgcaagtaaa acaaaatcaa cgttatttat tattgcctgt
    27061 tcaatgatat ttgataaaac ggtcagattc gtcttcaata gtttttcctg tacagtttta
    27121 tcaagagttg tcagtccttc aaatgaccga tccatatgta aatctgccgc atggataaat
    27181 ttcaccatta aaaatttcct cctttttttc ttttatcata ccattttcaa tgtttttttt
    27241 catcaataac cgaaaatttg ttcgtattat gcaaagaaat tacgataaat ggccgacttc
    27301 aaaaaataag tttattcgaa aaaaagattg tgacatgatt tttgtcacaa tcctaaatat
//
LOCUS       DADXTG010000026        26901 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.026, whole genome shotgun sequence.
ACCESSION   DADXTG010000026 DADXTG010000000
VERSION     DADXTG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.026"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1311
                     /locus_tag="IWI57_000948"
     CDS             <1..1311
                     /locus_tag="IWI57_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449108.1"
                     /translation="TEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLL
                     TEIVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVL
                     FGVYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAY
                     GNQPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFE
                     LDGAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRK
                     NWIFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYL
                     FLLVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYL
                     SDCLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1588..3648
                     /locus_tag="IWI57_000949"
     CDS             1588..3648
                     /locus_tag="IWI57_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBC2449109.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            3731..4300
                     /locus_tag="IWI57_000950"
     CDS             3731..4300
                     /locus_tag="IWI57_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449110.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4475..5281)
                     /locus_tag="IWI57_000951"
     CDS             complement(4475..5281)
                     /locus_tag="IWI57_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449111.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5419..6933
                     /locus_tag="IWI57_000952"
     CDS             5419..6933
                     /locus_tag="IWI57_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBC2449112.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7336..8319)
                     /locus_tag="IWI57_000953"
     CDS             complement(7336..8319)
                     /locus_tag="IWI57_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBC2449113.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8324..8971)
                     /locus_tag="IWI57_000954"
     CDS             complement(8324..8971)
                     /locus_tag="IWI57_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2449114.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9056..9688)
                     /locus_tag="IWI57_000955"
     CDS             complement(9056..9688)
                     /locus_tag="IWI57_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2449115.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(9704..10255)
                     /gene="coaC"
                     /locus_tag="IWI57_000956"
     CDS             complement(9704..10255)
                     /gene="coaC"
                     /locus_tag="IWI57_000956"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBC2449116.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10252..10989)
                     /locus_tag="IWI57_000957"
     CDS             complement(10252..10989)
                     /locus_tag="IWI57_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBC2449117.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(10986..11975)
                     /locus_tag="IWI57_000958"
     CDS             complement(10986..11975)
                     /locus_tag="IWI57_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBC2449118.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12233..12775
                     /locus_tag="IWI57_000959"
     CDS             12233..12775
                     /locus_tag="IWI57_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2449119.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            12792..13877
                     /locus_tag="IWI57_000960"
     CDS             12792..13877
                     /locus_tag="IWI57_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449120.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            13889..14695
                     /locus_tag="IWI57_000961"
     CDS             13889..14695
                     /locus_tag="IWI57_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449121.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            14696..15523
                     /locus_tag="IWI57_000962"
     CDS             14696..15523
                     /locus_tag="IWI57_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449122.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15520..16593
                     /locus_tag="IWI57_000963"
     CDS             15520..16593
                     /locus_tag="IWI57_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2449123.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17021..19717
                     /locus_tag="IWI57_000964"
     CDS             17021..19717
                     /locus_tag="IWI57_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2449124.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            19794..20282
                     /locus_tag="IWI57_000965"
     CDS             19794..20282
                     /locus_tag="IWI57_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2449125.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            20294..20788
                     /locus_tag="IWI57_000966"
     CDS             20294..20788
                     /locus_tag="IWI57_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2449126.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            20809..21618
                     /locus_tag="IWI57_000967"
     CDS             20809..21618
                     /locus_tag="IWI57_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2449127.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            21615..22436
                     /locus_tag="IWI57_000968"
     CDS             21615..22436
                     /locus_tag="IWI57_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2449128.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22487..24586
                     /locus_tag="IWI57_000969"
     CDS             22487..24586
                     /locus_tag="IWI57_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBC2449129.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            24583..25701
                     /locus_tag="IWI57_000970"
     CDS             24583..25701
                     /locus_tag="IWI57_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBC2449130.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            25740..26618
                     /locus_tag="IWI57_000971"
     CDS             25740..26618
                     /locus_tag="IWI57_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2449131.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            26764..>26901
                     /locus_tag="IWI57_000972"
     CDS             26764..>26901
                     /locus_tag="IWI57_000972"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBC2449132.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CM"
BASE COUNT         8562 a         4649 c         5649 g         8041 t
ORIGIN      
        1 actgaattca aaacaaagcc agagatccaa atttcagaag aagcagagat ccatgtcgaa
       61 cgtctgaatt tgacggatat caaaaattta agaagaatct actttcaacg atcaaaaatt
      121 gctcgactgt taacggagat cgtggattca aaagtaacga tcgaaaaatt tgcagagaat
      181 cattttttaa gcatatcaag agcctatgta aaaagaagag aattagtctc ttttcttgaa
      241 cagcatcaaa taaagttaaa gaaaaacata ttagttgggg aagaaataaa catacggaac
      301 gtattatttg gggtatattt cggggtcttt aatggttttt ctatgccatt tagagaaagc
      361 gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat atctgttcaa tttaaaactg
      421 tctgcaacaa aacgaatcaa attcaatttg ctaaccacta ttagtttgtt gagggcctat
      481 gggaatcaac caatcggaaa acgtttcttt tcagatgaat actttaagag ctcagaagga
      541 atgaagatca tcaaggattt atcacatatt tcttttcttc atatccctaa agagcagatc
      601 attgatgaga tttcttatat catgttgttt ttgatcacgg aagaagaatt tgaattagat
      661 ggcgcatttg gacttgcgga agctcatttt catgatatcc acatgattag tcaaaaaatg
      721 tcagtggaat tgattgatca attaccattt tcgattgaaa tctctgaaga agaaagacta
      781 tatatacgaa aagaacttcg tatgggtatg atgcatgtca atcgaaaaaa ttggatattc
      841 gactttgaga tgactagctt tacgtctaaa aagcagctac agtttttctt cgagacgtat
      901 ccaatattca gtacagcaat ccaaaaggtc atacaagaga ataaacgcta tttcttggga
      961 aaagatgaac gtttcatgat tcgcttattt tatgattatc tctttttatt ggtcggtatt
     1021 tgtccggtat catattttga agcaccgatt catgtctgta ttgatttttc gcaaggatct
     1081 gcttataccg attacattat ttctcaagtg aaaggattta aaaattacaa tattgtgatt
     1141 gagagtcgaa tcacttctcg tacgaatatt tatttatctg attgtttact tgaaaaattt
     1201 aataaagaac agataatttg gaaaaatcca ccgactccag atgactggga atattttgga
     1261 aataccatca tcagagaaaa aaatagacat ttaactgaaa aaatcagcta gtatttatgg
     1321 atggcagctt tgtctcacat atttattagc tgatttatgt ttttttcaaa gaaaaggtaa
     1381 ctagatataa ctagatataa ctagatatat tacttaacaa aattaaaaag tacataagtt
     1441 attattccga ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat
     1501 cttcttttga ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta
     1561 gacaagagta gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat
     1621 aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt
     1681 atgatgcgca cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag
     1741 gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct
     1801 atcactatta ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact
     1861 tcctataata aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa
     1921 acattatgga catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact
     1981 gatcctacaa aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca
     2041 gatataaagg acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag
     2101 tttgaaagaa agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta
     2161 gaaaggctga agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat
     2221 gggaatatcg tggatacgac taatacagga acatctacta tttccttgtc ctattttgat
     2281 ccaattgctt taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca
     2341 gctatcaaag gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt
     2401 gttgaatatg taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac
     2461 gggacagtag atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg
     2521 aaaacatcaa atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca
     2581 tcgactgtca tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca
     2641 catatgagta gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga
     2701 gtgccaaata tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat
     2761 tggatgcaag caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt
     2821 cactcagaac tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt
     2881 tatataccag acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa
     2941 ataaacaaag aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta
     3001 aaggatgtat cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa
     3061 acaggtgatt ttacgattat gtatagttat catgacccgt attcactcca agaactccat
     3121 gctcagtcac tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa
     3181 agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca
     3241 gggaaagtaa aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa
     3301 tctaataaaa cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat
     3361 gaaattttag agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg
     3421 gaagatcaga aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt
     3481 gattccaccg atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca
     3541 cctggtggag gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca
     3601 tgggtatacg ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta
     3661 tctgttatag tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag
     3721 ggatagaaaa atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact
     3781 tcaacaaaat gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa
     3841 agaagatgaa tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga
     3901 tgaagcactt gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag
     3961 gatgtttttt gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa
     4021 aaaatcaaga gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca
     4081 aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca
     4141 tacggacgga ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta
     4201 taaaaaaatg ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta
     4261 tgaaaaagtt atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc
     4321 tcagtattgg gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata
     4381 ctatcggata actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat
     4441 tgtcactgcc ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa
     4501 aattcttgat gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac
     4561 tgaaaacaac tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca
     4621 cgtctaagta accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt
     4681 caatgattgt ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact
     4741 ccacactatt gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat
     4801 ctgctgaaaa ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa
     4861 caactgctag taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa
     4921 caagggaaaa gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc
     4981 acattgaaaa atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa
     5041 aagataacaa aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa
     5101 aataagggat ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt
     5161 ggttccagac ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc
     5221 cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca
     5281 taaaataact ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct
     5341 atatgactta aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag
     5401 gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt
     5461 cttgctatga gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg
     5521 aaggaatctg ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa
     5581 atgaaagaat cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct
     5641 atcgaagcaa aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga
     5701 ggaaacacat ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa
     5761 gaaggaatca aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga
     5821 acgaatgata aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg
     5881 ctagatcaaa acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag
     5941 aggacacatc gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg
     6001 aataacgtcc atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag
     6061 aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag
     6121 gtcaccggaa aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt
     6181 gaaaaatatc gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga
     6241 gatggtatca ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa
     6301 attcatccaa ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa
     6361 ggagcaatct tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat
     6421 aaagtatctg cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa
     6481 ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact
     6541 ggaaagacaa tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa
     6601 acagttgatg gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc
     6661 acagccatgg aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga
     6721 atccattaca cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa
     6781 aaccagccaa ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac
     6841 aaccggatcg gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata
     6901 caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt
     6961 gaaatcagcg ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag
     7021 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta
     7081 tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     7141 gaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     7201 acggcggaag cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     7261 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     7321 ttgatcagca tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc
     7381 ttccatatat tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg
     7441 ccgaatcttg ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc
     7501 tgctttatac tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc
     7561 aatcaataca ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac
     7621 ccaacgtcca tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa
     7681 atccaaaata acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc
     7741 ttgtttttta tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc
     7801 ttctgatcct gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc
     7861 tgctgttccc acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt
     7921 tccaagccag taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga
     7981 aacatagtct agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc
     8041 ggcataacca cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat
     8101 atcactttta ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg
     8161 tttcaaagag actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac
     8221 tttgatcagt aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac
     8281 atctacagaa ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc
     8341 attcttggaa tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag
     8401 aaatatccgt ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg
     8461 tttttgatgc cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag
     8521 tcaatgtgac tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg
     8581 cataatgttt agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg
     8641 cataaacagc actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg
     8701 ttttgattgc gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa
     8761 aaatcaatac atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat
     8821 caaaagagac atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca
     8881 cccctgcaaa aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt
     8941 gtccgtttgc acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt
     9001 ccattcaata gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt
     9061 tagtaagaga agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa
     9121 gacctgcgac gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat
     9181 aaacagcatt taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga
     9241 tcatattcat cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag
     9301 aaacggaatc tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa
     9361 ccaccccaat caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt
     9421 ctgttccgat gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg
     9481 tgctacggat catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc
     9541 ctaaaacgat cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga
     9601 tagggataaa acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag
     9661 ttaatgtaaa gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat
     9721 taagagtcag catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa
     9781 aatctccaca agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca
     9841 atgtttccag attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg
     9901 caatcaattt tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg
     9961 caatgccgtt tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt
    10021 cagcttgttt tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt
    10081 ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc
    10141 tgcttttaga cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat
    10201 cagcgctttt gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga
    10261 ttcctccagt ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagcttc
    10321 ttccactgtt ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt
    10381 caaatcattt gcgaaaataa aatcagtttt gttatttatc agactatttt ttgcgactgc
    10441 cagaagagat tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg
    10501 ctgccattca cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg
    10561 ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca
    10621 attgtccagt tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga
    10681 aaaactaaaa gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg
    10741 ttcttttttc gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata
    10801 aatcgttata ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat
    10861 tgtggtatcc ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga
    10921 atgatttgta atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag
    10981 aatcttcatt tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt
    11041 agtttagatg ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct
    11101 gcaatctgca aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg
    11161 acagaatcaa ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt
    11221 agtttgattc ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga
    11281 agaagactgc ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt
    11341 cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct
    11401 agacttttta gccatgtgac agactcttct tttctggaaa aggcaatgat tgactggaat
    11461 cctagtttat gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg
    11521 acaacttttt tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca
    11581 gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga
    11641 actaaccagt ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca
    11701 agcccaaaac cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg
    11761 acgacagttc cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca
    11821 gactcgctca ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag
    11881 acttcatttt cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt
    11941 tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt
    12001 tcttttatca taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa
    12061 aattaggtga aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat
    12121 tcagcaaaaa aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga
    12181 gtggtagtaa acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat
    12241 tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga
    12301 acgcacagat ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc
    12361 aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca
    12421 tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga
    12481 tgaagaaaac gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga
    12541 gcctgtcttg ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc
    12601 tgaaacgttt atttttgtat tagaagggga attagagtta agcttaggag agacggtcta
    12661 tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa
    12721 tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag
    12781 ggggtaaaaa cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg
    12841 atgaaacagt attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc
    12901 ttggtccttc cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag
    12961 ctacagaagg ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc
    13021 gacaagtaaa tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata
    13081 acgttgcttt tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa
    13141 aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt
    13201 ctggtggtca aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat
    13261 tgctattaga tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg
    13321 aactgcggga tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag
    13381 aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa
    13441 gtggtacacc cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg
    13501 gggagagcaa tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta
    13561 agcaatttga atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac
    13621 gcccagagga tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata
    13681 ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat
    13741 ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc
    13801 cagaagatat ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg
    13861 acagctacga agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc
    13921 ttatattttg tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc
    13981 tttttttgat atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg
    14041 gacatcgctt tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac
    14101 gctgatcatc agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg
    14161 gctgatgctg attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat
    14221 tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc
    14281 gaaacagata ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc
    14341 atttatgatt atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc
    14401 tagtagagat ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc
    14461 attaaatggt gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat
    14521 gctgactcgt ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca
    14581 tttcttggtt acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc
    14641 tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa
    14701 aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt ttattcttat atgcaccgat
    14761 cttctatctg attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt
    14821 tacttgggaa aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa
    14881 tacgttcatg ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc
    14941 tatgggaatc tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa
    15001 catcttatta gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact
    15061 actaggattc ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc
    15121 aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc
    15181 ggctagagac cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct
    15241 ttctccagga atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc
    15301 ggttacattt ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg
    15361 tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc
    15421 catgatattg gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa
    15481 gaaaaacaga agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg
    15541 tttttaggta ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa
    15601 acaagtggca tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat
    15661 cctagtttga tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt
    15721 gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct
    15781 ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat
    15841 cattcgaaga tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat
    15901 ccagataatc agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat
    15961 aaattctttg atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa
    16021 gacagtttga tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta
    16081 ggctattctt tgaatagtaa gaatatggca cagctgacag aagcagcaaa taaattgaat
    16141 catttaacac caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat
    16201 gaagaagcct ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat
    16261 gaacatttac attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt
    16321 atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa
    16381 ccggaaaatg cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca
    16441 aaagcgttat tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg
    16501 atctcccatc ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac
    16561 ctattcttag aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact
    16621 tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta
    16681 ccaaaagcgg atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc
    16741 taccagagaa aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct
    16801 aagattgaaa aagttacaga aggataaagt aattggaaac tatgcaaata tacttaaccc
    16861 ttctgtaatc tattaataaa actaatatat gaatccagtt caatatttgt agctatgaaa
    16921 aaacataaaa atcatattag ataaaaacta aattggtaga taaattattt tgtcagatat
    16981 ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa ttggaataca aatatagaat
    17041 agacatattg cagccgatag tttattatgc ttttagacac attaaactgg aggaaaaaat
    17101 gaaaaagagc gctaaagatc gagtttttga tgagctgata cgactaacag ggaatatgtc
    17161 ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc tctcgtcaga atgccagcca
    17221 ttatttgacg agattagtgg aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt
    17281 gtggaagcct ttagatgaat atgcggttgt agataatacg gagcaaataa acgaagcctt
    17341 tcattctgta gttggtcatg acggaagtct gagagaggtc attcaaaaat gtatagccgc
    17401 tgtcaaatat ccgcctaacg gactcagtgt attaataaat ggagccacgg gtgttggaaa
    17461 aagtttttta gccacaaaaa tttttgaata cgctatccat gagcaaatta ttgaaaaaga
    17521 tgcaccattt gctatcttaa actgtgccga ttatgcggac aatcccgaac tgctttcggc
    17581 aacactgttt ggatacaaga agggggcctt tacgggggct gaaaaggata ccgatggttt
    17641 attggcaacg gccaacaacg gctatctttt tctcgatgag gtccatcgcc tatcgaaaga
    17701 aaatcaagaa aagctctttc tatttattga tactggaaac tatcgtccag tcggtgaaaa
    17761 tgtaaattgg cattctgcta aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa
    17821 ttatttatta gacacctttg atcggcgtat tcaaattagt gttatgttgc caacttttga
    17881 agatcgcccc attcgagaac gattagagct gattcaatta ttttttcaaa atgaagcgga
    17941 tgttttacaa aaagatatta ttgtttcaag agaagccttg tctttaatgt tatctcatcc
    18001 ctttagtggg aatattggta agctgaagaa tatcattaaa atcagttgtg cggatgtgta
    18061 tagtcgaaca caagaagaac cattaacgat tgggaaaaat gagatcttga ttcagttaaa
    18121 tatgctggaa tccgaagtag aaagcttgcc gattgctagt ttattgataa agaaaaatca
    18181 aaagataaaa acggatcgaa tcaaaccgtt tgattatgat tcgattttgg aaaaaattac
    18241 tcagctgata aatgaaggta atttcaatga aaatttttct tcgataaaga atgaagtaca
    18301 gcatttggtg catcacattg ataaggattc ttttttatca atgagtagaa aattgatgat
    18361 tcagatgttt agaaaggtct gggtatccgt tttaggaaaa acgtacggat tgacgacgag
    18421 tggacctgtt gcagaaatta ttagcagatc ctatgtcatt tccccaagct ataagacaga
    18481 cttaaccggg ataaatcgta cattagctgc aaatctacct cggacgactt acttatgtaa
    18541 tcagttcttg gagcatttac caccgctctc agaaaaggac gagaactttc taagactgct
    18601 tattgtcgtg actttatctg attatgtaga tgaatccatc gagctgaagg gcctgttgct
    18661 tgctcacgga gatagtacgg ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa
    18721 ttatgttttt gaagcgttgg atatgccagt agagagtaat gtgtcagaga ttgtcgaatc
    18781 gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt ctagtgctga tcgtagatat
    18841 gggctcttta aatcaaattt acacacagat caagaacgat ttaaaagggg agttacttct
    18901 tctgaataat ttgacgactt ccattgcact tgatattggc ttgaagatga cgatgaatgc
    18961 gccatttaaa cagattgcgg aggatgcagc agacaagtat tcaatcaatg ttcaatactt
    19021 tgaaggcttt tctcaaagta gcaacattat catttcctgt atgtcgggtt tggggatttc
    19081 ggacaaattg aaagatgttt ttcgtcatca tttatcggat gaatccatcg aagttttcac
    19141 gaaggactac agagaactac gtgaattaat cgaccaaaat gatgaaactt attttgacaa
    19201 gacaaaattg gtcatcacga cttcggatct tccacgttcc ttttcaattc ctaatattaa
    19261 tgtttatgac atcttagacg gtgatggagc agccaatttg gagcatattt tgagtcctga
    19321 aatgaagccg gcagaattta accgcttgat tcaggagcta gtaaaattct tttcgttgga
    19381 aggaattgcg gaccgtttga actttctgaa cccaacgatc gtgatgagtg aagtggaaac
    19441 agtcatttct aaatatgaaa attactatca tttcacttta aatggccgtg taaaactaaa
    19501 tctctatatg catacggcat tgatgatgga acgtttattc ttggctcgga cagaaaaatc
    19561 tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa ttttattatg tttctagaag
    19621 tatttttcaa tcaatggaga tgaaatataa ttttaaagtc aatgcttatg agctttcgct
    19681 gctctatgaa ttattaggac cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc
    19741 tgtttttttt aggttagaca ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt
    19801 taaaagttgg cacaggaatt gcttatataa aagtgaacag aaagaaagga attaaccata
    19861 tgaagaaaat tctaatagcg actcacggtc acttagcttc aggctttctt agcagtattc
    19921 aattactgac ggggaaggtt caagagatta cggtgattaa tgcttatgtg gatgagtgtg
    19981 attttgaaca ggagttgaat gtatttgtaa accaggtgaa cgagcaggat cagatttttg
    20041 tttttaccga tttatttggc ggaagtgtca atcagaagat taccaaaacc tttttggaga
    20101 aagagattgc tgtaactatt ttgtctggct ttaatctgcc aattctgctc gaaatagtat
    20161 tggcaaccaa ggaattaaca aaggaggata ttcaggaact ggtaaataaa tgccaacagg
    20221 aattaaagat gaccattctt gatgaggtac atgtagtaaa tgaagaggaa gatttttttt
    20281 aggaggaaga aaaatgatta aagcagtgcg aattgatgaa cgattaattc atggacaagt
    20341 agcgatgact tggacaaaga gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc
    20401 ggctaccaac gatattcaaa agatgacatt gaagatggct gcaccatcga atgtcaaagt
    20461 cattattaaa acagttgatg acgcgattca tttgttaaaa gatccgcggg cgaaggacat
    20521 gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta aaagttgcga aagctttccc
    20581 aacggaaatc gaattaatga acgtggggaa tgctgggaaa atgacaccgc catcggaaga
    20641 taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca gaaattcaag acttaaaaga
    20701 attagttgaa ctttatccag ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc
    20761 agctatctct attttgagtg atttttagaa aaaggaagga gaaaaattat gtatgatgca
    20821 tttgtagtcg ctttggccgt ttttattgga gttgctggac atgaaatgtt cgggatgtcc
    20881 atgctgagtc gtccgattgt tgttgctccg ttagcaggag cgttgatggg ggatttgcag
    20941 acggggttga ttgtcggggc gtcactagaa gccatcttta tgggtgtcgt caatatcgga
    21001 atctctagta cggcggagcc ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa
    21061 atgggaggaa acgtggatgc gattattccc attgtctttc cactagcagt attaggatta
    21121 cagttaatga atatgatttt ttcttttgta tgtggaccga tggctgctaa atttttcaaa
    21181 tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc acttctttat gtggtttgtc
    21241 cactatgcgc tttatgctct tattccattc tttgccgttt tatttggagc agatgttgtc
    21301 aaaagcgtct tggataatat tccagaggtc atcatgaacg gattaacagt cgctggaaat
    21361 ctgctgccgg cagttgggat ggcgatgctg ttacggatgc tttggagtaa aaatattgct
    21421 gtgtggtttt tctttggggc cgttgttatg gcgtatctcg agttaccatt gattgcaatt
    21481 gccgcagttg gaacgattat tgcaattgtt atagcccaaa gagatctaca actaaaaaat
    21541 atatctgttc atgcagttca aagcgatgct gctgtaaatg caacgcttga ccaagaagag
    21601 gaggatttct ttcaatgaaa ttagctgatg gaatttctaa agatgagaaa aaattggttc
    21661 ggaagatgtt ttggcgctcg atgactatgt atgcttcggt gaatccggtg acgatgggcg
    21721 gcggtggttt cgcctattct atgatgccct tcattaatca tttctacaaa agtgaagaag
    21781 atcgagctgc ggcgctggaa cgtcatacac agtatttttc gacgaccatt ccttgcgcgt
    21841 cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg
    21901 acgtggaaag tatcaacgcg atcaaaacga gtttgatggg accattggct ggaattggcg
    21961 attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg actagcgatc aacttagcca
    22021 atgccggaaa tattttggca ccaattatct ttttagttat gttcaatata cctaattact
    22081 tgatgcgata ctatggcggc gttttaggct attcattagg ctctaaatat attgaaaagc
    22141 tgtatgcgaa cgggttaatt gaaattctga caaaagctgc tagtatcttg ggtctgatca
    22201 tggtaggtgc aatgacgatc agtatggtca aattcaatac cgttttgagc ttttcaatga
    22261 agggcgaaga actaatgaat gtccaagaaa tcctcgatag tatttttaaa ggaattgtac
    22321 cgttattggt aacattgagc tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa
    22381 ttatttttgg aatggtcgtc ttgggaattt tattgtcatt acttggaatc gcatagtagg
    22441 aagctgcagc tactaggctg cagttttttt agcaaggagg gaaaagatga ctattaatga
    22501 agtaatttat tcacccaaac acccattttt taatgctgag ttaaacgacg gaaatgtgac
    22561 tacttcaaac gaatacagct actatcggaa tattcctgat gtaaagggat tgaccttcga
    22621 tgtaagtaat caaaagacat tagcaaacct tgatgtttgc gggacgattc aaacgttgac
    22681 cttttatcga gaaaatttac taactgagga aaaaccaggt gtttgggtca ataaacagct
    22741 ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac gggattgtct accatttgtc
    22801 agaatcggat catcgtattg aagtcgatgt gattcaagac tgtttgccta gatatattca
    22861 tcattattcg acgtttaagg tcattgtagt atcctttgct cccatactgg ctaataagcg
    22921 tttaagtatg cttgttcaac aggtttacat tgtgaatgaa acacacgaaa gccttcaagt
    22981 ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat
    23041 ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa gtggcaagct tcgccattgc
    23101 ttttgtggac ccaaatgctt ttgaagaagt aatgactttt caggaatctg atatcgaaaa
    23161 atggctgaag gaaacattga actattttgc tcagctattt gggcagctgt caatgccaaa
    23221 cgatagagtt gtccatctct atcgtcgtgc actttatcaa agcttttcta gtttcggtat
    23281 gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat
    23341 ttggaataag gatatgtatt acgcatcatt gccattcacc atgtttgaac cggagttatg
    23401 tcaaaaaacg gttctatggt ttgatcaata tggcgttaag tttcctggtt caaaatttac
    23461 aggaggtatc aatcattctc tgagtaattc gttatccagt gcgttactgg ctagtcttta
    23521 ttttgaacat actggggata caaaattctt tgagcggtgt ccgcaggttc tactgaatgc
    23581 ttcaagaatt attgaggaca tattagctca acgtcggtca ggggaaccaa tgctttttcc
    23641 atctgtttgg ttatcagacg cctttgcact tggaaagtat cataccggca gcaatctttg
    23701 tctttggaag gcctgtattg gacttgcaga aatttttgaa gccctagaac aactgagtct
    23761 tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac
    23821 tatcgatggt ccttttggtg agcaattttt ggagggaatc ggtgatgagg agaaaacaac
    23881 ctattcagtg aagcactatc aaaaaccgat tcttgagcaa gggttaatct ttttaagtga
    23941 tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat ggagaagaat cagacactac
    24001 gttgatgcct ttttatcaat ttttagataa taaagatcat ttatatcaga acaccatggc
    24061 cttttctgct agttctttca atccaaccta tagcgaggaa atcaaaggca tcacttgggg
    24121 attagaatcc ggcgccactt ttcctggctt tatcactgtt ttaatgagcg agctgcacaa
    24181 ccctcaagct ttcgtaaccc gattagaaga actaatttgt ttggcagatt tagacggttc
    24241 gtggtggtgg tggccgtatc gcttagaggc tcaacaagga gaagtcgtgc gagactttgg
    24301 ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc ctaatgatca gtcaatattt
    24361 cggattgaaa ttcaaaaaag gagtattgca aattgatcct gttgataatt taacgttttc
    24421 ttggaagaat ttgaagttcg gtcaagcctt catttcgatt gaatgtacgc aatccgagtt
    24481 gagtatttca aatttgtcag aaaagccatt gaaaataagt gatacgaaaa aaattcttca
    24541 cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa agatgagtat actaaaaaat
    24601 attacccaat ttaaaaaaat ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag
    24661 aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca
    24721 attccgctga cttctggtga agaaactgtg attgcggata ttactggaac gggaataatt
    24781 cgtcatatgt ggatgacgat tcgtgatcga acagaatttg gcagttttgt tttacgtgat
    24841 ttaatcttaa gaatttattg ggatgggtcg gatagccctg ctgttgaaag tcctttaggg
    24901 gatttctttt gtaatgggtt tggagagcgt tgcgaggtga actcgttgcc gatagtggtt
    24961 aatcctacag ggggctttaa ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt
    25021 acgatcgaga atttacatcc gaaggatatg tcctcatttt tttatacgat aaattacgct
    25081 ttggtggacg aattggaaga aaatagccta tattttcacg caaattggca tcgggaacgc
    25141 attaccaaat tacaggagga ttataagtta attgatcgat taaagggtca aggctattac
    25201 gtgggtactt accttgcgct tacctgccta gaacgttatt ggtggggcga aggcgagttt
    25261 aaattctaca ttgatgggga tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat
    25321 tattttggcg gggcgtgggc ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa
    25381 aactattcaa cagccttttt agggtatcct taccaatcaa atagagatgc cacaagagat
    25441 cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg gtgacgacgc aatgccacga
    25501 catggtcttt atcgttggca tcttcaagac cccatctgtt ttaccgaaga tttatcggtc
    25561 actttacaac aaatcggcaa cgatgatgtt cgcttatttg agcggcaaga cgatgttgct
    25621 agtgtcgctt actggtattc aacagaagct tcaggaaata ctaaacgtca tttcactgat
    25681 cgagaacgct tgccgcgtta atgaagtgtt tttcacatat tcgaagggag gaacctatga
    25741 tgatatatgg ggcaattgaa gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg
    25801 aattaacgat tttggaaaga gtcagttttc ccacaacaac tccagttgaa acaatgaagc
    25861 aggtcattga tttttttaaa ccttttaagg aacaattggc aggcatcggg gtcggctgct
    25921 ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa
    25981 aacttgcttg gcaaaatttc aattttgtag gaacgctaaa gcaatatttc aatattccga
    26041 ttgaatggac aacagatgtc aatgcggctt gctatggtga atatgttgct ggttctggca
    26101 aagggttagc aggtgttgca tacttcactg tgggaacagg tatcggaggt ggtgccttgt
    26161 tcaatgggac tttcgtggaa gggttcagtc atccggaaat ggggcatatg ttgatcaagc
    26221 gacatccaaa ggacaatttt tcaggaaact gtcccttcca ccaagattgt ttagagggga
    26281 tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa aggccagaat ttattggctg
    26341 acgattcgtt ttggcagatt gaagcctttt accttgccca atgtgcatac aatacaacac
    26401 tgatgttttc gccagataga attatttttg gcggtggtgt aatgaaacaa gaacacatga
    26461 agaaaaaagt tcaggataaa tttgttgagc ttataaatgg atatgttgaa ataccaccaa
    26521 ttgatagcta cattattacg cccgaattag gtgataatgc cggaataatc ggcggattag
    26581 cattggccag aaaagcggta agaaataagc aaccataatt aatgaatgtt tcattgatac
    26641 tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa
    26701 atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag
    26761 gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc tgtatacctt gaggatattc
    26821 actggtggcg tacacttaaa tagtaatata ttgtgttact ttatgtctgt ggtaatagtt
    26881 atagttgctt accaatgtat g
//
LOCUS       DADXTG010000027        26716 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.027, whole genome shotgun sequence.
ACCESSION   DADXTG010000027 DADXTG010000000
VERSION     DADXTG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.027"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            140..2374
                     /locus_tag="IWI57_000973"
     CDS             140..2374
                     /locus_tag="IWI57_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2449133.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2522..2842
                     /locus_tag="IWI57_000974"
     CDS             2522..2842
                     /locus_tag="IWI57_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBC2449134.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLSV"
     gene            complement(3047..3270)
                     /locus_tag="IWI57_000975"
                     /pseudo
     CDS             complement(3047..3270)
                     /locus_tag="IWI57_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(3468..5048)
                     /locus_tag="IWI57_000976"
     CDS             complement(3468..5048)
                     /locus_tag="IWI57_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBC2449135.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(5449..6825)
                     /locus_tag="IWI57_000977"
     CDS             complement(5449..6825)
                     /locus_tag="IWI57_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBC2449136.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(6953..8071)
                     /locus_tag="IWI57_000978"
     CDS             complement(6953..8071)
                     /locus_tag="IWI57_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2449137.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            8213..8647
                     /locus_tag="IWI57_000979"
     CDS             8213..8647
                     /locus_tag="IWI57_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449138.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(8725..9258)
                     /locus_tag="IWI57_000980"
     CDS             complement(8725..9258)
                     /locus_tag="IWI57_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBC2449139.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(9352..10530)
                     /gene="mutY"
                     /locus_tag="IWI57_000981"
     CDS             complement(9352..10530)
                     /gene="mutY"
                     /locus_tag="IWI57_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBC2449140.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(10523..11320)
                     /gene="recX"
                     /locus_tag="IWI57_000982"
     CDS             complement(10523..11320)
                     /gene="recX"
                     /locus_tag="IWI57_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBC2449141.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            11427..12809
                     /gene="rlmD"
                     /locus_tag="IWI57_000983"
     CDS             11427..12809
                     /gene="rlmD"
                     /locus_tag="IWI57_000983"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBC2449142.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(12983..13561)
                     /locus_tag="IWI57_000984"
     CDS             complement(12983..13561)
                     /locus_tag="IWI57_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBC2449143.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            13883..14353
                     /locus_tag="IWI57_000985"
     CDS             13883..14353
                     /locus_tag="IWI57_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBC2449144.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(14461..15687)
                     /locus_tag="IWI57_000986"
     CDS             complement(14461..15687)
                     /locus_tag="IWI57_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBC2449145.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(16441..16641)
                     /locus_tag="IWI57_000987"
     CDS             complement(16441..16641)
                     /locus_tag="IWI57_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2449146.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(16775..17383)
                     /locus_tag="IWI57_000988"
     CDS             complement(16775..17383)
                     /locus_tag="IWI57_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBC2449147.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(17468..17938)
                     /locus_tag="IWI57_000989"
     CDS             complement(17468..17938)
                     /locus_tag="IWI57_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBC2449148.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(17986..18900)
                     /locus_tag="IWI57_000990"
     CDS             complement(17986..18900)
                     /locus_tag="IWI57_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBC2449149.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            19046..19849
                     /locus_tag="IWI57_000991"
     CDS             19046..19849
                     /locus_tag="IWI57_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBC2449150.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            20027..20974
                     /locus_tag="IWI57_000992"
     CDS             20027..20974
                     /locus_tag="IWI57_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBC2449151.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(21042..21335)
                     /locus_tag="IWI57_000993"
     CDS             complement(21042..21335)
                     /locus_tag="IWI57_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBC2449152.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(21363..22028)
                     /locus_tag="IWI57_000994"
     CDS             complement(21363..22028)
                     /locus_tag="IWI57_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBC2449153.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(22168..22800)
                     /locus_tag="IWI57_000995"
     CDS             complement(22168..22800)
                     /locus_tag="IWI57_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2449154.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(22807..23874)
                     /locus_tag="IWI57_000996"
     CDS             complement(22807..23874)
                     /locus_tag="IWI57_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBC2449155.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(23871..24602)
                     /locus_tag="IWI57_000997"
     CDS             complement(23871..24602)
                     /locus_tag="IWI57_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBC2449156.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(24771..25250)
                     /gene="greA"
                     /locus_tag="IWI57_000998"
     CDS             complement(24771..25250)
                     /gene="greA"
                     /locus_tag="IWI57_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBC2449157.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(25386..26561)
                     /gene="mltG"
                     /locus_tag="IWI57_000999"
     CDS             complement(25386..26561)
                     /gene="mltG"
                     /locus_tag="IWI57_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBC2449158.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         8104 a         5370 c         4421 g         8821 t
ORIGIN      
        1 ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct taaaaaacta
       61 ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac ccatttttgt
      121 gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg caacgattca
      181 cttgtatgcc actgtcaatg gtcagcggac acaactcgat tactgtcaga gctgttatca
      241 aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg atccatttgg
      301 atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac agcaaggaaa
      361 ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg gtggtcagcc
      421 acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca cagaacaagc
      481 acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga
      541 aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa
      601 aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt
      661 aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta ctggtatacg
      721 aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt
      781 gatcctattc atagatgaag ttcacgaaat cgtcggtgct ggttctgctg gtgatggaaa
      841 tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc aaatggttgg
      901 tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag aacgtcgtat
      961 gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt tgaaaggttt
     1021 gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca tcgaagcagc
     1081 cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag ccatcgattt
     1141 gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc ctaaaacaat
     1201 cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag aagaagattt
     1261 cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga aagaacgtca
     1321 gctcactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca
     1381 acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt
     1441 agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc gagtagcgaa
     1501 agcgattcgc cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg gctccttctt
     1561 atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg cctacgaact
     1621 attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg aaaaacatag
     1681 tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt
     1741 aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag tagaaaaagc
     1801 tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat tgacagatgc
     1861 acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta atgccggaac
     1921 tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt
     1981 attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg acgggatcat
     2041 cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa tgctagacga
     2101 tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga atgtcaaaga
     2161 aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc gacgcacgat
     2221 ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt ctatccatga
     2281 gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac cagaacgttt
     2341 ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg tgagacggta
     2401 tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact gttcatttta
     2461 taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag aggtgagcgc
     2521 catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc agcttgaaaa
     2581 cactgatgcc catctagtca aggtctacac agccggaaat accactattg tctacactga
     2641 agcccctgaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga
     2701 aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga
     2761 tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt
     2821 aggcgctaca ttatccgtgt aaacaaattt tgaacataat aattaaagag cgtgggacaa
     2881 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcgaaa gcagaagcaa
     2941 ctccttcgga aataagctga aattcacgaa aattgctttt caaatttttg tgaattccag
     3001 cttatttctt ggagttaaac gcttctgtct cagcctcttt ttctttctag ctttttgatt
     3061 tttctctttt cttttgtact tttctctttt cattttcagt tctacgcgtg tctttatttt
     3121 taactttttc ctctttatgg atgattgatc gtactttttg tgcaatttgc tcgtttttgt
     3181 cccagtcttt tacatctaat aatcgacata atcgtaacat ctcttctttt tctgtttttt
     3241 tctttcacgt ctttgccatt ctgtttccat gtgtcacact ctcctttctt taatcatatg
     3301 tacgcaaaat aatttttata tttttttgat tagacatttc tatgatttta atatagtaaa
     3361 aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa tatgagaggc tgtttttgtt
     3421 tggctattct tattacttga tgcagggcag cttgtgtcaa gacttcttta catcaaactt
     3481 tttaatttta cattgggata tttatccgca aaccagcgtt ctgcaaattg gttctcgaat
     3541 aagaacaacg gttggtcgaa acggtcacga gccaagatat tgcgacttga actcatcttc
     3601 tcatccaaat cttctggatc gatccagcga gcgattttgt gtcccattgg agacatgatt
     3661 acttcggcgt tatactcatt taacatacga tattggaata cttcgaattg tagctgacca
     3721 acagctccaa tgatatattc ttccgttaga tatgttttat aaagctgaat cgctccttct
     3781 tgaactaatt gatagattcc tttgtggaag gatttttgtt tcatgacatt ttttgcagtc
     3841 actttcataa acagctctgg agtaaatgaa gggagttctt cataagcaac ttttagtttt
     3901 ccttcgaaca atgtatcccc aatctgataa ttgcctgtat catatacacc gatgatatcg
     3961 cctgctactg ctttttcgac gttttctctc gaatctgcca taaattgtgt gacattgctt
     4021 aatttcagtt ttttatttgt acgttccaac cagacatcca ttcctcgttc gaatgttcca
     4081 gaacagattc ggacaaaagc gattcggtca cgatgggctg gattcatatt tgcttggatc
     4141 ttgaatacga aacctgaaaa ttcttcctca taaggactta ccatctgatc atcttgtgtt
     4201 ttgtgtccat aaggagctgg tgccaattca acgaaagtct ctaaaaaagt ctgtacaccg
     4261 aagttagtca atgcagaacc aaaaaatact ggtgtctgat cgccacgagc aattttttct
     4321 tgattaaatg catctccagc ctctttgatt agttctactt cttctaagac ttgttcatat
     4381 aaggaatttt cagttagagg atgattttcg ggaagcagtc catcttcatc caattctact
     4441 aatcgttcat cctgataatt ttccggacga taaaactcta cacgattatg gtagatatca
     4501 taaagacctt ctagcccttt acccataccg attggccaat tcatcggata agattcgata
     4561 tctaatagct cttctaattc ttctaacaat tccaatggtt cacgaccatc acgatctagt
     4621 ttgttgataa aagtaaatat cgggatgcca cgttttttta caacttggaa taatttcttt
     4681 gtctgtgctt cgatcccttt tgcactatcg attaccatga ccgcactatc cacggccatc
     4741 aaagtacgat aagtatcttc agaaaaatct tcatgtcctg gtgtatccaa gatatttact
     4801 cgtttccctt gataatcaaa ctgcataaca gaactagtaa cagaaatccc acgttgtttc
     4861 tcgatttcca tccagtctga tttggcaaaa ttacctgttt ttttgccttt tacggtcccg
     4921 gcttgacgga tagccccacc aaacaatagt aattgttctg taatcgttgt tttcccagca
     4981 tccggatggg aaatgatcgc aaaagttctg cgattgtcca cttgttgctt taattttgga
     5041 ttattcataa cctaactcca tttcttccat tcaattattt atattttctc taggcacaga
     5101 ttgtccgatc tcttaaagaa agacaatttc atatagggct gtgacaaaag ccgtgtagca
     5161 gtctcttttt ccgagtaaac ggcgttctag agacagccta tcagcaataa gcccacccaa
     5221 gccaaaaata cgaaaagctg tttttgcttg gctgctctta ttacttaatg caggatagct
     5281 ttttcacaac ctttgtcaac tactacaact gaatcagtat agcatggatt cagcgaatga
     5341 aaaagccacc aaaccataag agtcaaatat ttttttgtca gctgactaaa agaaaagacg
     5401 tgttgcttgt ttattcaagc aacacgtctt tcataaccaa gtttccaatt atcttcttgg
     5461 ctcgtcatct tcgaattctt ttctgaagta tcttcgttct tcttcaggtt ccgcttctgt
     5521 ttcttccgga tcatgaaaaa tgacacggat tttaaggatt cgagaacctt ccatttcttc
     5581 agaaaccagc gtaatattat cgacatcaaa cgataacttt tcgccttcat ccggaatcat
     5641 gcctaatgct gtaatcaaat aaccagccat cgtatctaca tcactcatat gcagatcact
     5701 gtcaaaagct tcattgaact catcaatcag catacgtcct tggatgatat attcgtgttc
     5761 atcgattttt tcataaagat tctcaacttc atctgattca tcatcgattt ctccgacgat
     5821 ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg ccgccatatt catctaataa
     5881 aatagccatc tgattctgcg tctttttcat ttcatataac agatcatcaa taaagaccgt
     5941 ttcaggaacg aataaaggct cctgcatgat tttcttgata tctaaattat caaagccgaa
     6001 tttatgggca gcttttagta aattctttgt atgaaggatt ccgactactt tatctttgtc
     6061 ttcgttataa acagggattc ttgaatagtt ttcggataaa acttcattca cattttcttc
     6121 cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt gggaccatga cttctcgggc
     6181 aactttcgta tcaagagaaa aaaccccttg gagcatttct aattcttcat tattcaatac
     6241 gccttcgttt tcaagcatat aacgcatttc atctctggtc atttttgaat cttcatcatc
     6301 aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa gcagaaagca gccagacgaa
     6361 gggcttagct actacaccaa tcaagcgtat cagccctgaa gtgaactttg cgacttcttc
     6421 tgatttattt aaagcaatac gtttagggta caattctcca aaaacaatcg atacataggt
     6481 caaaatagcc agcacaatga tatttgcaat attttttgcc gctgtgccgc cacctaatac
     6541 cggagctaac cgttccgata aagtattagc tagtgaagca ccagacaaaa tgttgaccaa
     6601 agtgattcct acttggattg tcgataggaa attatttgga ttctgcaata tttttaaaag
     6661 cttttgagat ttcacatctc cttcatcagc tttttgctcg attcgattct tattgatcga
     6721 aacaacagca atttcggaag ctgctaaaaa agcattgatc aatgtcaaaa cgatcaataa
     6781 taaaatctgc gctaacagcg actgactctc agggtcagca ttcataatcg cattctctcc
     6841 ttataaaatt aatttttagt ttacgataga aaaaaacgtg gcactatagc cacgttaaaa
     6901 tggtatcaca aagcaatttt ctttgcaata aaaacaagtg tactaaaact acctacgagg
     6961 tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc tttgacgatt ttgattatat
     7021 aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc taagaagata ccaagcaatc
     7081 cggcaatatt tccagctact agaagaacga taatgatcgt taatggatgg attttcagtg
     7141 atttcccaat tacattagga taaatgatat tcccatccaa ctgttggacg attagcacaa
     7201 ccacacaaca aagtaatgct tttaccggat cattgaagac tgttactaga aatgcaggtg
     7261 ctagtcctag ataaggtcct aaatatggaa ttaggtttgt caatcccgca atgaccccaa
     7321 ataagaaagc ataatccaca ccaattacca tatatcctaa aaatgtgaag gtaccaacga
     7381 ataaacattc gattgcttgt ccgctaatgt aattagccaa tgttttattc agttgcccca
     7441 ataactccac aatttggttt cttctttttt ctgggaaaaa ctgttggatg tttggcacta
     7501 gtttttctcc gtctttcaac atatagaaca agatgaatgg tgctgtaatg attactacgg
     7561 ttgccgaagc gatcgttcca acgatcgaac ctaaactatt ggatactcca ctaaggaatt
     7621 gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa atatttattg atatctactt
     7681 ccttgaacaa aggcaattcg gctacctctt ttaaccagct ttctacctgt ttgatgaaat
     7741 caggcatatt agatgccaat gaggaaattt gctgtactag acttgggatg acactaagta
     7801 aaatccaaat aattgctgca actaaaagga gcagcacaat tgcgacagcc catattcttt
     7861 tgatttttgc tttcatcaac agcttgacaa taggattcaa taaatagtaa agaaagcctg
     7921 caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc gatcggttcg aaaataaagt
     7981 ttatttctga tgaaatcatg atcaatgcag ctatgatcaa taattcaagc gaccaaaaca
     8041 ttagtttaga ctgtctaagt ttttcaaaca atagatttcc tcctttcttt cttactagta
     8101 tatattatat tcgttaaaaa ttcttttatt ctttttttat ttttgaaaac ttgccatact
     8161 ttttatttgt tttattttcc agtatactat acgaaaggaa ggtggctaga ccatgaaagt
     8221 tgtacaaaca aaagatacaa tgagcaatat ttacttagat gctgtacgga tcaggcatca
     8281 agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa atagacaaag atgaagccca
     8341 ttgtatccac ttcgtcctgt actcagataa aaaagaaccg caaggaactg tacgtctttt
     8401 gcctttagaa aacggaaaaa tgaaattaca gcggatggcg attctttctg aatacagaca
     8461 ccaagggtta ggaaaaatat tgattgaaga agcagaaaac tttgctaaga atcaaggcta
     8521 taatacgatt ttattagggg cacagtccac agctgaaact ttttacgaaa aactaggata
     8581 tacagcttac ggcgatcctt ttgaagatgc tggcatgcct catatttata tgaaaaagac
     8641 tttataaaaa tttatttcga aaaaagcaaa aaacagcaag cttttgagaa aaatctctca
     8701 ctggcttact gttctttttt gtgtttactt acgcgacaat tgcttgtaac ggttatacca
     8761 aatatcaata tagccttccg agaacgggcc atctccatta ttgatccagt caaccagaat
     8821 cttcacattt tcttttagga tgaagtcaat atcgttcggg taattcatca ttttgctgtg
     8881 catctcatat tcatcgacat ccaatagtcg tttctcgccg tcaggaaata ccttgatatc
     8941 caagtcatag tcgatatatt tcaaggcctc atcgtccagc aaaaaaggtg aagctagatt
     9001 acaataatag gaaaccccct tttctctgat cattgctatt acattgaacc agtatttttt
     9061 atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg cgtccatcag attcagtgac
     9121 taatgtgtga tcattcacgc cgatcataga gtgttcgctt gtttttaata ccatggtatc
     9181 tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt tggatcgtca caaactctcc
     9241 ttcttttgga actcccatag caacccttcc ttaagcagca ttttgtgctt tgtgtttggg
     9301 tgttcattac accgacatca taattattat atcaataaaa atgagctttg cctagaaagg
     9361 attcctattg tttcgatttt tctctaactg atctaccagt ttcatttgga ccttaggaaa
     9421 aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa gttttgttct cggtaaattc
     9481 ttgttctgct ccctcggttg cccgtccgta aaaaaggaga acatgccatt tcagatgact
     9541 aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa acaaagagtt cctgcttttc
     9601 aaatgggaga ttttgctctt cttctgctac taggtcatca aacagatcga tttctggctt
     9661 tgtttcccat tcttttttca atcgctcata ctcctcttgt gtcacttcca tcattggaaa
     9721 tgtccacata ttcgccagca atttttggct atctctttct tcaaaataat acgctccaga
     9781 atgattttga agagctgcac tgatataata aacgtctttt ggctttgctt tttttgtttt
     9841 tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa caaaaggctt ggatcggaca
     9901 agtctcacac tttggagaag ttggtgtaca gatagctgaa cctagatcca tcattgcttg
     9961 gttgaattca cctggatgct tttcatcaat gattttccgc atcgcttcat caaagatttt
    10021 cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac aatctgctga ctactcgcat
    10081 cacgttgccg tcaactgcag gttctggaag tccaaatgcg atacttgcaa tcgcgcctgt
    10141 cgtatatggt cctattccct tcaatgagct aatttcttca ggcgtttgcg gcatttcccc
    10201 atcaaattct gacatgatct gtttagcagc agcttggatg ttccgggctc ttgagtaata
    10261 accaaggccc tcccatgctt ttaaaagctt ttcttctgga gcatttgcta gctcttcaat
    10321 tgtaggaaac cattccatga aacgataaaa ataatcgatc actgtgtcta ctctggtttg
    10381 ctgaagcatg atctcagaga tccagatacg ataaggatca cgattatagc gccaaggcag
    10441 gtttcttttt tcttgttcat accattgaat aaactgatct tgaaattctt ttgtttcttt
    10501 atctgtccat ttttcccatt ctttattcac tttcctcttc cttttccgta atgaatcgct
    10561 ggatcatatc caaatcaaat cctttttgat ataagctttg ctttactttt agatttcttt
    10621 ttttaggttc aaaccttaaa tttcttcgcc atagtttatc tccttgtttg actaaataat
    10681 catattctat ttcttgatct gcttcttgcg gaagatcagc tatcgcttgt tggatcactt
    10741 cttgagaaaa gcctttcgtc atcaatgttt gttggatttt tctcaataat tctttttggc
    10801 tttttccgtg attttttcca aacgcttttt cggctgtctg atgtgcattt tgaacttggt
    10861 cttcaaatgt atattggctg agcgcttgtt caatgatttc aggtttcaaa cctttttgct
    10921 gcatctgctg cgctaatttc tttggtcctt tgtcacttaa gcgcatattc gtgcgcacat
    10981 aactttcccc atagaccaaa tcatcgacaa ggtccaattc ttttaagcgg gctacgattt
    11041 tatttcgatc ttcttgaagg atttcctttt cttttaagta tgttcggatt tctttttccg
    11101 atcgcaactg ataacttaag taattcattg ctaggtgaaa accgaaatca gctccgctac
    11161 tcttcttgat ctcttggata gtttcttcgt ctaattcccg tccttttaat aatcgatagc
    11221 gtactaatac atcttcagat acttgtaaag cttctccttc agataaatct acgcgataga
    11281 aagggccttt gcctttactg attctcgtaa cggttaacat aaacatcctc ctcgaacata
    11341 tattctctat cataccacag aaaatccccg taatcaggta ttatatgtta gaataaacca
    11401 cagaaaaaag aaaaagggag atacttatgg cagaaacaat tgtcaaaact ggacaaacgg
    11461 ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg aatcggttat ttcaaacggt
    11521 tgatcatatt tgttccatat gcactaccaa aagaagaagt actagtgaaa atcacgaaag
    11581 caactcctcg ctatgcagaa ggagaattaa taaaagtcaa aaaaacaagc aaagatcgcg
    11641 tagtggcacc atgtcctgtt tactatgaat gcggtggctg tcaattacaa cacttagctt
    11701 atcaagctca attggatttt aaaaaggatc tgcttttgca agcattggaa aaattcaaac
    11761 cagcaggctt tcgatcttat caacttttgc cgacgatcgg gatggatgag ccttggcatt
    11821 atagaaacaa agcacaattt cagttacgaa aaaataagca gactcaaaaa attgaagctg
    11881 gtttatacga agcaaactct catcagttag tccctataac agactgcttg gttcaagaac
    11941 ctcgaacaca aaaagtaatg aatactgttg ttcaactatt gaataaattt gatgcaccca
    12001 tctatgatga acggacaaac agtggaatct tccgcacaat catggttaga atcggtgtta
    12061 aaactgggga gttacaagtt gtttttatta ctcgcagtaa aaaattcccg caaaaaaacg
    12121 caatgatccg tgagatcaat gaacgactgc ccgaagttgt ttcaatcatg caaaatgtcc
    12181 aggcacaaaa aacgtctctc gtgatggggg atgaaacgat ccacttatgg ggaaaagaag
    12241 ccattgaaga acaaatcaat gaagtgactt tcgatctttc tcctcgtgcc ttctttcaac
    12301 tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat caaagcatta gatgtccagc
    12361 ctaatgaaac gatcgtggat gcttattgcg gagttgggac gatcggctta agtgtagcaa
    12421 aacaagccaa agaagtacgg ggaatggata ccgttcctca agcaatcgct gatgcaaaaa
    12481 aaaatgcgga aagattagga tatcacaaca ctcgatatga agtcgggaca gcagagcaag
    12541 ttctgcctaa atggttgaat gaaggttttc agcctgatgg tatcatcgta gatccaccac
    12601 gtacgggttt ggataacgca ttgatccaat cattattgaa ataccctcca aaaaaacttg
    12661 tttatatctc ctgtaatgtt tctacacttg ccagagatct ggttaattta agtaaaaagt
    12721 tcaagattga atatttacag tccgtcgaca tgtttcctca gactgcacgt tgtgaagttg
    12781 tcgtgaagat gacgagactc gctgactaaa tgaaagaggt tgtgaaaaag tctgccctgt
    12841 atcaagtaat aagaacagcc aaacaaaaat agctcttcat gttttatgaa tactgtactt
    12901 gtgaccgaag agttaacagg aggacactat ttatttaaaa aaagggttgt gacatagttt
    12961 ttgtcacagc cctttttgat tattattgga tattcacgtc tatcacttga taaaatgcat
    13021 ttgtcgtatc gctgatattc catactgcta aaatcacatt atatcctttt cgatcctgcg
    13081 gaagtgtgat cgtttggttg actgtttttg gtggaacagt tgctttgtcg tctatttttg
    13141 tgatcaattc aaaatcacta aatttcaaag gttgattagg attccaagtt ggtttggtaa
    13201 tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa attccaagta atatttaatg
    13261 ttcctggatt aaccatgttt ttatgccatc tgttagatgt ctgttcatct aaaggctcaa
    13321 agccgctgac tcctgcacta gcaatttttc catcaatgaa tgtatttttg gtagcttcga
    13381 tactttgcgg ttcccattgt gctcttccga cattttgatt taagtttcct gctgctgatg
    13441 ttcctaggta tgcacggctt cctggagaag taatataacc atgcgcaaaa cctggaatgc
    13501 taaaaccgaa caaccctaaa ctaactaacc caataaacaa taacgtacta atctttttca
    13561 tgtagaaacc tccttcattt gataaccaca ttgtaaacgc tttattgtat taacacaaac
    13621 attatgcttt tataatttta tgttgaagta aaaaaggtga aaaaagtaag atgcagatta
    13681 taacagaagt agacgagaaa agtacaaata gaaaacatga gaatcaaatc atatgatctt
    13741 tttcaaaaac atcattcata taaacaaatg ttcaactttt taataacaac aattatcaat
    13801 ttttttctta cttcacaaac taaatacagg tcttgctgtg agccttcatg gtaaactaat
    13861 attatagaaa ggagttagta aaatgaagat gataaaagat attcgttttt gggtttgtgt
    13921 tataggaatt gtcatattag gctttttatc agggttgctt agcggaaatc ccggaaaata
    13981 ttactattct ttgcaacttc caccatttgc accacctagc tggatatttg ggccgatgtg
    14041 gaccttactg tatatattga tgggtatttc tctttatttg ttacttaatc acaacaacaa
    14101 aaaacagcga aacaatcttg tcggtctttt tgtgattcag ttcattttta attttatatg
    14161 gtctgctcta ttttttaatt taagaaatat tttcattgca gcaatcgata ttacactttt
    14221 agttattttc ttgtcagtgc ttatgtatca gttgtggctg catcatcgcc tgacaatgtg
    14281 gctcatgatt ccttattatt tatgggtact attcgcaact ctattgaatt attcaattta
    14341 ttttttaaac taagcgctaa aaagagacgt cctgtaattt aacacataga aaaagtgcca
    14401 cctaagttag attttttgtc taatttaggt ggcactcttt attaaccaaa ctattttttt
    14461 ctagtaccca cttgttgttc catactcatt attctggtca taaccataac cattttgttc
    14521 ctgtccagcg taaggatcat aactttgagt atcttgactt ggtgtatagt actgacttgt
    14581 atcttctgtt tgctgataat aggtattcgg atcttggtac tgatcatatg tgttttctga
    14641 ataggaagaa taatcggtgt acccttcact attcgatcca tcaccgtttg tcgaatcgcc
    14701 atcttccgaa tcatcaaaga acatcattga agcattctca tttttcaaat taggtaatgt
    14761 atcgcttgtt tttatattta attgtttctt cagttcattt tggatcgaaa gaagatcatt
    14821 ttttcctaaa atttgatagt agatatcatt gatcatttgt ccttcgcctt gcaactggtc
    14881 ttgtttgatt gtttcaaacg ccggggtgta gttcgtcgct acatcaagca tgtcatccca
    14941 atctaagtcc gtcgtcacat tgttctcaac tgcttttaaa atttttctat aattgcttac
    15001 gccatctaaa cttaaaactt tattgacgat tttcgtcact acttcccgct gacgacgttg
    15061 tcgaccgata tcgccttctg gatcttcgtg tcgcattcga gaataggcta aacctttttc
    15121 tccattcaat gtttgttcac ctactggtac atggacacca tccaaagtaa agtcaatttt
    15181 attattcact ttgatgccac cgaccgcatt gatcaaatct tccatgccat ccaaattgat
    15241 cgttacataa tgatcaatag gaatatcgag cagttgctca attgaatcca tggccatttc
    15301 gacaccacca aatgcgtagg catgatttaa tttatctacc gttccataac ctacgatatt
    15361 cgtataaata tctcggtcca aactgatgat cgttgatttt ttttgtttcg gattaactgt
    15421 tactaccatc atcgtatcag aacgtccttg ttccgttctt cctaatccac cggtatctaa
    15481 acctaatagc aaaaccgaaa aaggttcttt gtcatcaatg cttactctct cagaacgttt
    15541 ggaggatatt cgattagagt ttttagaaat tttgtttact gtttggctcg cttctcccat
    15601 aaatctgatt ccgtatgctg ataatccggc aatcagcaaa acgaataccc ctaacaaggt
    15661 taatagaact ttttgccatc gcttcataat acacctctgt ttcacatttt ctaaaatatt
    15721 gtctcatata actgttcaat cagcaaattc actttttaat cataagaaaa ctttaaaaaa
    15781 catttttgtg taacgattat tattttttaa aatttctcaa aattcttaac tatcatacca
    15841 taccgtgaaa aagatcgaaa gaccgaatac acgcagttcg caatcgtttt atcttttttt
    15901 atgtattttt tccagaaaaa gcgaacaaca aggtattttc caaataatcg tgcagtaaaa
    15961 tgatcgcaca tacctttccc atctttttaa cagtctttgc ctaccgttca gattatcatt
    16021 cgatttcacg acaatttctt cctatgactg gtccttttgt taaacccatc ttctattcgt
    16081 tttatttcca gctcactgca tggctcttaa aatgactcga actagtgatt gcattaccat
    16141 catcttttaa tggatagata tgctcctctt caaagtaaaa cgattttctt ttaaagtaat
    16201 agaattgaca tggtaatgag tatgtatact agtggtttca caaatggtct tttattcaac
    16261 atccaaaaac tgaaatttct ttaaaatcca ttactaacga agaaaatgtt ccttgcttga
    16321 taattagtta ggacatgtca agtgaacaaa tgcatttact tagccaaaaa atctaaaaac
    16381 ttcataaata gacaaaaaga cacgactctc taagaagtcg tgtctttatt caacaaatta
    16441 ttataattta gtcacttcag cagcttgagg tccgcgtgca ccttcaacaa tcgtgaattc
    16501 tacttcttga ccttcttcta ggctcttgaa gccgtctcct tgaattgctg agaaatgaac
    16561 aaatacgtca tctccgccgt ctactgtaat aaacccaaat cctttttcat tgttgaacca
    16621 tttaaccgtt ccgtgttcca taaatgtata tcctccaaaa aagttcttgc aagtaacctt
    16681 gcaagaacat tatacccaat ttagaaaacg ctagcaagct ttcgctcttt tattttccaa
    16741 attattttta agactgatat gacggaggaa atggttattt tgctgcttcg aaacgtttgt
    16801 ttacttcatc ccaattaata acattccaga atgctgcaat ataatctggt cggacatttt
    16861 tatatttcaa ataataagca tgttcccaaa cgtccaaacc tagaattggt gtctttcctt
    16921 ccatcaatgg agaatcttga tttgcagtag aggtaatagc taattttcca ttttccatta
    16981 caagccaagc ccatccagaa ccaaatcgtc cagcggctgc ttttttgaat tcttctttaa
    17041 aagaagaaaa atcaccaaaa gcttcattaa tcgcttcttt tatttctcct gtaggttcgc
    17101 caccagcatt tggtgccatg atttcccaga aaaatgaatg gttagcatgt ccgccaccat
    17161 tgttacgtac agctgtcttg atatctgttg gaatagcgtc catatcagat aataattctt
    17221 ctattgtttt ttcgcctaat tctgggtatt tctcaattgc tgaatttaaa ttcgtcacat
    17281 aagtattgtg atgcttatca tgatgcagat gcatcgtttc ttcgtcaata taaggttcca
    17341 atgcgtcata tgcataaggt agatctggta atgtgtaagt cataaaaaaa ttcctcctca
    17401 atgttttctt aataatgtct acattacaaa gagtaacaaa gagaaggaaa ttcttcaatt
    17461 gttatgccta gtcgtctttt tcactttgac ctacttcttc ttcaaaaacc tcagtttctt
    17521 taaaaataaa caacttgctg aaaatatagt tcgcaaggat aacaataatt tgagtgatga
    17581 ttttcgccac atactcattg ctgttcataa cattgatcaa caataccatt gcccccatgt
    17641 ccaatccgta agaaaggata cggtagaaga agaacttacc gcattcaata agtgcatgtg
    17701 taaaattcgg tgattttgaa tggaataccc aaagtttgtt cgttacaaaa gcaaacaata
    17761 cagaacaaaa ccacgaaata gaattggata aggccatact taaatcaaaa tattggcgtg
    17821 cgagagcaaa actaacaaaa tttacaatcg tagccaaacc accaaaaaag agatagacta
    17881 gtatctccca atatcctttt gcttctaaat atccttttaa ctgtcgatac tgcttcatga
    17941 gccaacctac cttattaaaa attcttattt atcatactct atatttcaat aactttcatc
    18001 tttatttaaa aaaattaatt ttttttgatc ctttgccttt gtctctgctt ggatcgtcac
    18061 atgtgtaata ccatatttta attttaataa agaaattata cgaggatata tcttttcaat
    18121 ctcgcttagt agctgatctt caaaatcaat atgtccgtcg aaaataatta tcttttcgct
    18181 atactgccaa atatggacat gatgaacatc ctttacccca tcgattgctt caatttcttg
    18241 ttccattttt tcatagtcga tagcaggtgc actttgtatc aaaatagcgg cagctttttt
    18301 gatcaccatt atcgcttcta tcaatatata tatcgaaatg atcagtgtaa taactggatc
    18361 gatccagtag atatcgaaaa aacggatcaa aattgctccg ataatcacac ttaccgaaga
    18421 cagtgtatca cttaacaaat gcaaataact agattttata ttcaaacttt catctgcttc
    18481 ttgtcgtaat aataatactg agaacaaatt cgcgagcaat ccaataactg ctactgttag
    18541 catcaaatga ctatttatct tttctggtga agtgaaccgc ttgaatgctt caacggttaa
    18601 aaacaaggat aatatgatca atatgaatga gttgatgaaa gcactcaata tttctaaacg
    18661 ctggtagcca tacgtacgtt ttttatttgc ttcttttcgg gccatttttt gtgccccgta
    18721 agaaaaaata attgccaatg tatcactaag gttatggaaa gcatctgaaa caagagacaa
    18781 actccctgac ataatccctc caataaattc tgataaagtg atcaccgcat tcaaaaccac
    18841 tactccaaat agtttttttg atgatctctg tggctgtgta ggcactattc cttcctccat
    18901 tttatttata ttggatagtc tttagacaag aataacattt ttttgatatt tcaccaatca
    18961 ttcagctgtt tccttaaagc acttgtattt cacttgatac ttcgctagaa ttgcaagtga
    19021 tgacactact agatgaggag tacttatgac aacaaaacaa ttaattatca gttcctttat
    19081 acgattcaaa gacctaaagg aggcgagaaa tgtcccattt tggaaagcca ttgtctatct
    19141 tttagttttg agtataatta tggctttgcc aatctctttt caagtttttc aagtcttaaa
    19201 caacattcaa ttggatgggc aaaagattgc tactcgcatt cctgatttta caatccaaaa
    19261 cgggcagctt caaaccgaag aaaaagaagg attcatttac caaacaaact ccattatctt
    19321 cacatttgat ccagagggaa aacgaacaga acaggacatc tctactgatt tgatggggaa
    19381 ttttgttagt gtcgggatgt taaaaaacaa acttatcata gctcttccta atacaggcac
    19441 gacttctgct ttgttgaaca ataaccaatt agaacttccc tacaccaatg aatctttaaa
    19501 aaacttaaca ggtaagcagc tccgttcttt tttaagtgaa gcatctatac cactttggat
    19561 aaaagtcctg acatttatat tttctattta tcctagtttt ctgaatctgc tgatcacttt
    19621 gctatttacc aacattgctg cttttctcta tgcacgcttt cgactaacaa aagctacttt
    19681 tcttgattgt ttaaaaacat tgatctattc aatttcattg cctactataa ttgcaacaat
    19741 cttgatggtc tttcttcctt cttttgacac tagcgccttc attgcttttg caggaatctt
    19801 tatttttgct caagctgtca aaggatgggc aaaaatatcg atcaattaag aaataaagac
    19861 aactcgttat ttctacttca atagaccctt ttagaggttg tgacattttt ttgtcacagc
    19921 ccctttttta tttgaaaaat atttctttct caaaaaacaa gcaaggcgct tcaaagttct
    19981 tcttgtatgc gctacactta gagtaaagaa aggcgggaaa ttaactatgt cagaaagaat
    20041 catttatgca gatcgtccat ttcgtgatga atttatcgaa gaaatccata ctatagcgcc
    20101 tggttatatc tttaaaacag aattacaacc agaggatcta caaaatgtag agatcagttt
    20161 gggctggaac tcagactacc aagaaaaact attagcctct tctaccttga aatgggtcca
    20221 atctatctca gctggtgttg atcagctacc ccttgagact ttttcaaaga aacatatttt
    20281 actttcaaat ggtagtggca tccacagtga atcgattacg gagcatgtct taggaatcat
    20341 ccttgggtat tctcgtggtc tttttcaagc acaaaaggca cagagtgaca tgaaatggtt
    20401 aggttcatcg gtccattacc aatctatcca agggaaaaaa atactaatca ttggaactgg
    20461 ccatattgga aaaatgattg caaaaaaatg caaagcatta gacatgaact gctatggaat
    20521 caatacatcc ggtcatccag cagaaggaat ggaaaagact ttttctttaa atcaactaaa
    20581 ggaagttctt cctgaagtag atttcgtagt caatatctta cctctaacgg aggaaacaac
    20641 tgggctcttt gatcagcata tgtttgaaca ctttgatcca aaatctgtat tcatcaatgt
    20701 tggccgtggt gcttctgtta agacacaaga tctggtgcag gctttaaata accagcaact
    20761 ttcctttgca gcattagacg tttttgagga agagcctctt ccacaagatc atcctctttg
    20821 gaaaatggac aatgtactga ttacttccca cattgcaggt ttgacacctg attttcaaaa
    20881 gaaattaatg gctatttttt taaccaatct gaaaagctac tttgccaccc atgagcttca
    20941 aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg aaaacaatat tttcaaaaga
    21001 aaaaagagaa ggtgacaaaa attgtgtcac cctctctttt tttattgttt tttaaagtga
    21061 taggttggct cctccaggtt gggatcataa tcaacaagta aatcatatcc tttaaagaac
    21121 cattcatctg cttcctcgat aaaaaaaagt gtcccatcca tttccacttt ctttacagga
    21181 tgttctggct gttcaactga cattccaatc gaaaaaccat catgaacctc tgttttgccg
    21241 tagacttttc caaaaaaacg tattccgtaa gtttctggca gatcaatttc tgagtcaaac
    21301 cacttttgtg cttttggcgt taccgttaat ttcatcccaa caacctactt tctcttttct
    21361 ttctattgat tcatcttatc gtctagaata tccacatcgc ctgtctctcc aataaccagt
    21421 agattatcat tttcacgaac atattcatct gctgcaggag aagcgataac cgtttgatct
    21481 actcttcgta tggcgactac tgttaagtca aaacgttgcc tgaaatttaa ttcagctaat
    21541 gttttgttga aaaatttagg attggagact cggatttctg ctaaagaata tttatctgac
    21601 aactctaggt aatctaaaat attctttgac actaaattat gcgcaatcct tattcccatg
    21661 tctcgctcgg gatggacgac gcgatcagct ccgatctttt caagtactct ggcatgatac
    21721 tcattttggg cttttgctaa aatatttggt acgcccattt cctttaccat cagagtaact
    21781 aagatactgg cttgaatatc ttctccaata gcgacaatta catgatcgaa atttcttatg
    21841 cctaatgatc gaagtgtcat ttcatcctgt gcatttgcca caacagcatg agtggctata
    21901 ttcatatatt cattgacccg atcttcatta ctatcaatag ccagtacttc ttgaccagct
    21961 tcgatcagtg ttcggcagat acttccgcca aatcgtccaa gacctacaat tgcaaaattt
    22021 tgtttcaccg ttttcctcct ttagtatctt cttttctcag tataacaaag cctttacaaa
    22081 aacaaaagca agcatcatta ttgtaaaaaa gccaagaaaa ataatagtta ttttctcagc
    22141 ttttgattat cctctttatc atcgttttta tttcaccaaa ccatgtttga atgcatagat
    22201 agcggcttgt gtacggtctt ccacttctag cttagccaat atattggaaa catgtgtttt
    22261 tactgttttc agtgtaataa acaattcgtc cgctatttcc tgattacttt ttccttcaga
    22321 aatcagcatt aaaatttcat tttcccgatt cgttagttct tcatgtaata caggctcatt
    22381 tcgccgactc attttttcca tcatttttgt tgtcacttct ggttctaata ctcgctcgcc
    22441 acgttgcgtt gcccttatag catcagcaat ttcatgtgcg gtagaggtct tcaacaaata
    22501 ccctgctgca cctgcttcaa tggcaggata aactttttcg tcatcaataa aactggttac
    22561 gataatgatt ttagcttctg gccaatcttt taagattgct tttgttgatt cgataccatc
    22621 catttcttcc atgactaagt ccattaaaat gacgtccggt cgaagtgtca tcgccttctc
    22681 atagccttgt cgtccatttt cagcttctcc aattacttca atgtcttctt gaatagataa
    22741 ataagaagag acgcctaagc gcaccatttc atggtcatct actaataaaa cttttatcat
    22801 ccttatctat gcctccttca tcaaagggac cttgatttct acacttgtac cttgaccttt
    22861 aaaactaatg atttttactg ttccaccaat accagctact cgttctttga tattgttcaa
    22921 tccataactt cccgttttcg tttcattcat attaaaccct gttccgtcgt caataatacg
    22981 taacaaaaga ttattgtcta ttttgtgcaa atatacctct aattcattgg ctttagcatg
    23041 tcttaatgta tttgataaca attcttgaac gattcggaac aagtgatcct caatggaact
    23101 agatagtttt acatcttcta catcccattt cagcgaaatc tggattttat tttgcaattc
    23161 cttcaataac tgctcgatac cttgctttaa acttttctct tctaaattga ctggacgtag
    23221 atgaagaagc agcgcacgca tctcagactg ggaagcgttg atgatctctg ctaccatttt
    23281 caactgcttg cgaaacattt ctggtgtctc gcttttttct gcttgctccg tcaatgccga
    23341 catcatcatc attgctgcaa acaattgttg cgaaactgaa tcatgcagct cacgggctaa
    23401 tcgatgtctt tctaattcta aaatttcttc tttggtttgt ccatctacat attggggacg
    23461 gctcgttaca atttgtaatt cactagatac ttcaatcatt ttttctttta tcttagtgat
    23521 ttccttatca atatctttga tgtataaatc gtccgatgca cttgggatcg gaagaagcaa
    23581 caatttggat tcataattgc cagaagaaag tgcacgcagc ttttcttcga ttttcccgta
    23641 ttttgtcttt tgaataagtg ataataatag aaagacaatt aatcctacac ctaaggaaat
    23701 ggctaacaaa tggaagataa gcggaacata taataaacgt gcacgcagta attctcccca
    23761 ccaatgactt tgattgctgg cataaaaata agtgaaaagt gaaaatagaa taataaggaa
    23821 agccgcaatc cccgaataaa cagctagcat cgctctggat atttttccca tcatattcgt
    23881 atcacctcga catctccaat caaggtatta gtaataatct tcaatctacg agggttttca
    23941 tcataatcac tgctgtagat tttgatttgt tcatttttca aagaaaattg ctcttcttca
    24001 aacaagacat ttcctactaa tgtcgcatgc tctaatcgga tggctacgcc caaaggtact
    24061 aagatccgtg ttctgcctat cccttttctt acaatcacaa tattatcatc ttttggcaaa
    24121 agcgtattgc ccaaatctat gatagtatca ccagaaatga cggcgatgtt gatatcgtcc
    24181 cattcataga cgtcgtttcc aatacgttgg ttaccgaata attgctgtct tttccgctca
    24241 ttattatgtg tttttaattg ctctgtttgg atcatcatga tctgtttctt tcgccaaaaa
    24301 gcatttttgg ttaaatctat ccctgaaatc tctacacctt tcagaccaat aaataaaacg
    24361 gcaaatacag ccatcatcca taaagcagga ctgttgacta agctaaaaaa aatgaccaaa
    24421 cttcctaata tcaattgaaa attttgaaat ttagtacgag gatgtttgcg catagctaga
    24481 tatatattga aaataccgaa tatcactaaa agcaaaagtc cagtattgtt tacgatttgc
    24541 caaacagcaa ataacagcag tagtgcttca actaccacaa aaaaacgcca agaactattc
    24601 atcgctcttt ctccctcctg acacattctc atttcatcat acctattttt ttaactgttg
    24661 gctataggac tttagatgta agatcgtttg ttgaaatcta aaatacacaa agagaaaaag
    24721 atctagagtg tatcatcagt aagatgaact ctctagatct ttcagtcaga ctacgcttgt
    24781 tctactttga cgattttaac taacatatcg ccacctggag tagcaatagt tacttcatca
    24841 tttttacgtt tgccaatcaa tgcttgcgca ataggagaat cattggaaat tttgccagaa
    24901 aacggatctg cttcagcgct tccgacaatc gtgtactctt cttcttctcc atctggtagt
    24961 tcgatgaatg taactgtttt accaattgat acctcatctg catctacacc gtcgttatcg
    25021 atgatttgag caaaacgaat catattttca attgttgtga ttcttccttc aacaaatgct
    25081 tgttcatctt tcgctgattc atattcagag ttttcggaaa gatctccgaa accgcgggca
    25141 attttgattc gttcaatgat ttctccgcgt ttgactgttt ttagttcttc taattctttt
    25201 tctaattttt ctttaccttc tagagtcatt ggataaactt tctctaccat gtgtaacgtc
    25261 tccttttttc gataaaatac cgcaaaaggt aatcttttga ggaatatagt ccaaaaaaga
    25321 ttaccacgct tttcttcaaa tgtaaatgtt tttctaaaaa tgatcaatgt ctagagcctg
    25381 tttttctaag aattatttac atatttttcc actaattcca tatgttcttc ataagtttta
    25441 gcgaaataaa catttccagt gctcgtatct gctacgaaat aataatagtc attttctgct
    25501 ggttctaata ctgccttgat tgcctcttcg cttggattgt caaaaggtcc aggtccatag
    25561 ccggtattgg tatacaaatt atatggtgaa tctacagctg tatcttcata cgtcactaat
    25621 tctttgtgtt cacctaatgc gtataaaaca gagatatctg actgaagtgg catatttgct
    25681 ttgattcggt taaagaagac ttgtgcaatg tttttacgat cattttcttt tacgccttct
    25741 ttttcaacta gagaagctag agttaaaact tcttgtacag tcaaattttt ctgcttgata
    25801 gctgaataat agttttgcat gaccgtattc gtcttgttca ccatttcaat cactagatcc
    25861 ttaagcgaag tactcgtgta ataatcatat gtggctggga ataagtaacc ttccaaacgg
    25921 tattttgtgt tctcagcttt agatgcactt tccaacagtt ctgggaattt ttggtgcaac
    25981 tcgttaaaga aagtctcatc attcactaaa tctaaaaatt ctttcttgtc tttaccagtt
    26041 accttcgcta cccgttcagc aatctgatcg atatcgtacc cttctggaat ggcaattttc
    26101 ccatctgcaa cttttgttgg ctcagaagta ccgccttctt gcaactgttt gccaatctcg
    26161 tcaagagtca tattaggcgc taactgataa tagccagctt gaaagccagt taaattttta
    26221 aattttgtat aataattgaa aacaatcccg cttttaataa tattgtcctt ctccaaaatc
    26281 tcaccaatct gcttgttaga agagccactc gggatctcga cttggactaa ttggtcatct
    26341 gatttatcta aaggcttcaa cccgctatct acataacgat acacggtaaa tccaagaaat
    26401 ccgccaataa tcaataatgt aagagcaatc actaaaatga tcttcctcac aatcttatct
    26461 tcttttttac gcagatttct ccgcttgtcg attatatctg ctgtagctgc cttactggct
    26521 tcttttttat ctttacttgg gtgttgtttg tttcctgaca aaacaactcc tccttactat
    26581 caagttcgta actttattca ttatacatga tatttgaatt ggcgtgaatg attttattta
    26641 aatattctat caaaaatgta agtagtaaat ccattactat aagattaaaa taaatgttat
    26701 caagatacaa gctatt
//
LOCUS       DADXTG010000028        26458 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.028, whole genome shotgun sequence.
ACCESSION   DADXTG010000028 DADXTG010000000
VERSION     DADXTG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.028"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            123..3650
                     /locus_tag="IWI57_001000"
     CDS             123..3650
                     /locus_tag="IWI57_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBC2449159.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3647..7369
                     /gene="addA"
                     /locus_tag="IWI57_001001"
     CDS             3647..7369
                     /gene="addA"
                     /locus_tag="IWI57_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBC2449160.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7438..7779
                     /locus_tag="IWI57_001002"
     CDS             7438..7779
                     /locus_tag="IWI57_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2449161.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8088..9131
                     /gene="pheS"
                     /locus_tag="IWI57_001003"
     CDS             8088..9131
                     /gene="pheS"
                     /locus_tag="IWI57_001003"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBC2449162.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9136..11556
                     /locus_tag="IWI57_001004"
     CDS             9136..11556
                     /locus_tag="IWI57_001004"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBC2449163.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11726..12736)
                     /gene="trpS"
                     /locus_tag="IWI57_001005"
     CDS             complement(11726..12736)
                     /gene="trpS"
                     /locus_tag="IWI57_001005"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBC2449164.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12990..13727
                     /locus_tag="IWI57_001006"
     CDS             12990..13727
                     /locus_tag="IWI57_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBC2449165.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13741..14556
                     /locus_tag="IWI57_001007"
     CDS             13741..14556
                     /locus_tag="IWI57_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBC2449166.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14569..15216
                     /locus_tag="IWI57_001008"
     CDS             14569..15216
                     /locus_tag="IWI57_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBC2449167.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15232..15888
                     /locus_tag="IWI57_001009"
     CDS             15232..15888
                     /locus_tag="IWI57_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBC2449168.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16050..16871
                     /gene="racE"
                     /locus_tag="IWI57_001010"
     CDS             16050..16871
                     /gene="racE"
                     /locus_tag="IWI57_001010"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBC2449169.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16874..18229
                     /gene="rph"
                     /locus_tag="IWI57_001011"
     CDS             16874..18229
                     /gene="rph"
                     /locus_tag="IWI57_001011"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBC2449170.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18230..18748
                     /locus_tag="IWI57_001012"
     CDS             18230..18748
                     /locus_tag="IWI57_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBC2449171.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18855..19355
                     /locus_tag="IWI57_001013"
     CDS             18855..19355
                     /locus_tag="IWI57_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2449172.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19635..20555)
                     /locus_tag="IWI57_001014"
     CDS             complement(19635..20555)
                     /locus_tag="IWI57_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBC2449173.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20720..21733
                     /locus_tag="IWI57_001015"
     CDS             20720..21733
                     /locus_tag="IWI57_001015"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBC2449174.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21843..22598)
                     /gene="fabI"
                     /locus_tag="IWI57_001016"
     CDS             complement(21843..22598)
                     /gene="fabI"
                     /locus_tag="IWI57_001016"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBC2449175.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22818..23258
                     /gene="fabZ"
                     /locus_tag="IWI57_001017"
     CDS             22818..23258
                     /gene="fabZ"
                     /locus_tag="IWI57_001017"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBC2449176.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23373..24134)
                     /locus_tag="IWI57_001018"
     CDS             complement(23373..24134)
                     /locus_tag="IWI57_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2449177.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24323..25417
                     /locus_tag="IWI57_001019"
     CDS             24323..25417
                     /locus_tag="IWI57_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBC2449178.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25503..26426)
                     /locus_tag="IWI57_001020"
     CDS             complement(25503..26426)
                     /locus_tag="IWI57_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBC2449179.1"
                     /translation="MIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGKTALDYWH
                     QYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVSCGMRTLYRLFSK
                     GIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIH
                     HKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSN
                     WKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRKVNQTFISSVSNQ
                     RNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8725 a         4592 c         5560 g         7581 t
ORIGIN      
        1 tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga atatagaaca
       61 aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga aggaggaaaa
      121 gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa gtcttgatcg
      181 acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc cttgttccca
      241 actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga agaagcggaa
      301 aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg gcttggtatt
      361 tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga tctgcaatga
      421 tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc ggagaaatca
      481 ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt acaggaaata
      541 ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa gaccagcaac
      601 ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg actcaacgtg
      661 cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca caagatttca
      721 acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag tatcaattac
      781 tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat cgtccttata
      841 cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt tatttccgtc
      901 tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt gcaccagagc
      961 agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca tctagccaaa
     1021 agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg gaagctgaaa
     1081 atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc agtgaagaaa
     1141 actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat ggaaatatcc
     1201 ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa caaatgatgg
     1261 aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc tattcttatc
     1321 ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg gaagcagaaa
     1381 ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg gacatcactg
     1441 aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa aaagactggc
     1501 agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat ttggaagaac
     1561 agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg aaaaaaataa
     1621 agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg atccaaagtg
     1681 gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga aacttagaag
     1741 cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat gaatatgtag
     1801 taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt acaagtggtc
     1861 tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga atcaatcgtt
     1921 tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg aatgatcaag
     1981 tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga gaagtattaa
     2041 atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt atctcttttg
     2101 agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat cttagttatg
     2161 cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt agaatcacag
     2221 aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc gaccctaatt
     2281 gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat cgattagctt
     2341 tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta gtactgaatt
     2401 cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta aatgttcctg
     2461 tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat acttcaattt
     2521 cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt ggtttgaaat
     2581 tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt tatcatgaat
     2641 cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg aatatgtccg
     2701 atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga gaacttcggt
     2761 ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta ggacagacga
     2821 ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg catcctcttc
     2881 agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt ctggaattac
     2941 cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc gatgtagctt
     3001 ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga cgttcattcg
     3061 atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg gacgtagcat
     3121 tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga tcatattatt
     3181 tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat acattgaaaa
     3241 aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta ctagatcatt
     3301 cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg aaagatgaat
     3361 accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg caactattca
     3421 tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc ggagaaatca
     3481 aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt ccattcagaa
     3541 gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg gagaaaatag
     3601 ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga atcaaattcc
     3661 tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt ttgatcaagg
     3721 agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc ttgttcgaag
     3781 agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg tgacatttac
     3841 tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag aatccgtcaa
     3901 tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt tattgcccac
     3961 agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt attattactt
     4021 aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga tcttgatgaa
     4081 ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg aacgattttt
     4141 ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga atctggtgtt
     4201 ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag aacagttaag
     4261 cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa aacaaatccg
     4321 tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga tgactcagtt
     4381 agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac aacagcagat
     4441 caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg ctggtttgga
     4501 acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag aaatttccaa
     4561 tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa gtaaaagtta
     4621 ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac cgttagtcga
     4681 agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta aacgggaaaa
     4741 aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca cagaaaaaac
     4801 gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat tcaaagaagt
     4861 aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat actggctaag
     4921 agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac aatccatcta
     4981 ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt tctcaaagaa
     5041 agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag aagtcctttc
     5101 ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa tcaattatga
     5161 cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac agtttgagcc
     5221 ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc cgacggatga
     5281 tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa ttcgtcaatt
     5341 aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc cgatagagta
     5401 caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt tagagatttt
     5461 caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc aagcgacaga
     5521 aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag atattccgct
     5581 tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag ccagcattcg
     5641 attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag catcaaacga
     5701 agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt ggcgagaact
     5761 tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga cgggatatct
     5821 agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt atgcattggt
     5881 tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt tcgtgcgttt
     5941 cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt caatagaaga
     6001 taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc cagttgtatt
     6061 tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt atgcttttga
     6121 agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt tatatgatac
     6181 gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg aagaaatgcg
     6241 taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg gttcctataa
     6301 aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg agttagtttt
     6361 tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg gttacggatt
     6421 gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt ctcttcttca
     6481 acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca ttgaacaaag
     6541 acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag aagatgaaac
     6601 tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc aagcttcttc
     6661 acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag atccagatga
     6721 tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat cccgtcaatt
     6781 tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc ggcaagtgtc
     6841 agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat tagagatgcc
     6901 aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt tagtagacga
     6961 aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa cagaacttgg
     7021 tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt ccatgctgct
     7081 tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat taatccacgg
     7141 aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt ttaaaacaga
     7201 ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa aatatcaagg
     7261 acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag tagaaaatgt
     7321 tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat tataattgag
     7381 caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt gagtatgatg
     7441 gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt ctctatttta
     7501 ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc gcaacgcttt
     7561 gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga acgcttgaag
     7621 gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa gagccgttca
     7681 gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca aatacaagtt
     7741 tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa aaatcttgta
     7801 aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt tggctaaacc
     7861 agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa tgaagttcac
     7921 tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt aagagtgcgg
     7981 tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc ttaacaggaa
     8041 tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg tctctacaag
     8101 cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca caagatttaa
     8161 atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc acagaagtat
     8221 tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt ttcgcaaatg
     8281 agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta gaagaagaag
     8341 cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt agaaaagtaa
     8401 aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat atttttgtgg
     8461 gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac aatttcgaac
     8521 ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc tatatttcag
     8581 acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg gaaaaacatg
     8641 atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc agagatacag
     8701 atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt gataaaaaca
     8761 tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg tttggggaag
     8821 atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca gtagaagtag
     8881 atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac actggatgga
     8941 tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca ggaatcgatc
     9001 cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct atgttgcggt
     9061 atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta agtcagttca
     9121 agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt agatttatcg
     9181 aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga agtagaggga
     9241 gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa ggaatgcgta
     9301 ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga agacgaactg
     9361 tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat tgttgcactt
     9421 cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg tggccaagta
     9481 tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt tataccgaaa
     9541 gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga tccagttttt
     9601 ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa tcgtgcggat
     9661 gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca gactcctaca
     9721 ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat tcaagtggaa
     9781 gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg agtaagaatc
     9841 cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg tccaatcaac
     9901 aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact acatgcattt
     9961 gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga aaatgaaaca
    10021 ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt cattactaac
    10081 ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga gatcacgaat
    10141 gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat tcgtcgtaca
    10201 gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg aatcaaccat
    10261 gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct tgcgggtggg
    10321 gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt atcagtgaag
    10381 atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa agaagtaaat
    10441 gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta ttccgtagta
    10501 gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga agttgctcgt
    10561 atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt tgctggaagc
    10621 ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg aagcggattg
    10681 agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt tgttataaat
    10741 gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt attacgaatg
    10801 aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa aaatacagag
    10861 attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg tacacatctg
    10921 ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga aaaagattgg
    10981 cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga aaacttattt
    11041 gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat gaaggaaatg
    11101 catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt tgtcggtcaa
    11161 gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc tgagtttgat
    11221 ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt gacaaaattc
    11281 ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa tcaggaatta
    11341 acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt atttgatgtc
    11401 tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct gacattcgtc
    11461 aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa agtcgaaaaa
    11521 gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag taagtaacga
    11581 tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact tcgtcacatc
    11641 tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac aggcctttac
    11701 agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt gctgatttta
    11761 cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta cgtaagattt
    11821 ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc ggtgcaaatt
    11881 cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt ccgccgcggc
    11941 ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca tccaagtagg
    12001 tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg tttggatcag
    12061 tatacatact catgactttt ttctgtaaga catcggcagg atcggagata taaataccgt
    12121 ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta cccattcctt
    12181 ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga ttgaagcttt
    12241 gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca ggtacaagat
    12301 tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag aatcctgtag
    12361 gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga ttgcgccgaa
    12421 cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat tccggaattt
    12481 gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa tagtctaaag
    12541 caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct gtcagtgctt
    12601 gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata gccactcggg
    12661 tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga tctcccgtta
    12721 aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc acttaatatc
    12781 ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga aagaggataa
    12841 aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt tctaagaaac
    12901 tttacaaatt actaaactat cagatataat gtattttatt atacatcttt aaaagaaatc
    12961 ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat cacgtggata
    13021 aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag aaaggagaag
    13081 ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc tgtatcaatg
    13141 gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg catgataaaa
    13201 agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat ttcaatttgt
    13261 acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg ttgaagcaag
    13321 accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat atgttagata
    13381 agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg gcaatcgcca
    13441 gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct gctttggatc
    13501 cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac ggtatgtcaa
    13561 tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt gttatcttca
    13621 tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc gaaaatccaa
    13681 atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag gaggaaaaag
    13741 atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt tcttgcacta
    13801 gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag caaatctacc
    13861 aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat ggatattgag
    13921 agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa aaagatcctt
    13981 ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat tccattattg
    14041 aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga acgaaaaaaa
    14101 caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt aaagaaagga
    14161 agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc agtcaaaggt
    14221 tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt agaattagaa
    14281 aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat gactacggat
    14341 aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt tggtggaacg
    14401 tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt cttgaaagct
    14461 gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat ctatcaaaaa
    14521 tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat gagcgaattg
    14581 atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt ttcgagtgtc
    14641 ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca attatcaaag
    14701 aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa cattccatta
    14761 ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa tctagatgga
    14821 ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc tgaaacagta
    14881 cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc ttctggtttt
    14941 acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat cgttatcccg
    15001 ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc aatcgtagct
    15061 ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa taccttcgat
    15121 acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc ttacttaatg
    15181 gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata catggatttt
    15241 agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa ggttactgtt
    15301 gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac aggtgtgatt
    15361 cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga tattatacgg
    15421 aatcttccat tattattgat cattttcttc acttactttg ccttacctca aatcggcatt
    15481 cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc agctatgttg
    15541 tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga agcaggatta
    15601 tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca agcatttaaa
    15661 tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac ttcattagca
    15721 gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg acagaacacc
    15781 aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt atgttacgcg
    15841 ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata gatgattctt
    15901 ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc gaaaaaaaga
    15961 gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa agtacacaat
    16021 agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat cgccctatcg
    16081 gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg aaacaattac
    16141 cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc cctagacccg
    16201 cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag caaggaatca
    16261 agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa atcaaagctg
    16321 ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca gtaaaaaaaa
    16381 cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt caagcttatg
    16441 aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct tgtcccaaat
    16501 ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa attgtggcag
    16561 aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga tgcacccatt
    16621 atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa ctgatcgatt
    16681 ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat ttgagcaatt
    16741 caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc aaacttttcg
    16801 aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat atcgaattgg
    16861 gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca aactggagct
    16921 tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg gtgatacaac
    16981 agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag aaacaggaac
    17041 aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga gaaatcgtag
    17101 agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc ttattggtcg
    17161 ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca tcgtagactg
    17221 cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg catttgtggc
    17281 attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag atccaataaa
    17341 agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg tcacagattt
    17401 ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga cagaatctgg
    17461 aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg aacagttgaa
    17521 tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc aaaaacatgc
    17581 tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa caatcatcat
    17641 tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag aagcgggata
    17701 tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa ctggcagcac
    17761 atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg atcagccagt
    17821 cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag gaatctattc
    17881 tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat tattatatga
    17941 attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt tagtattcgc
    18001 ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag tagcccgtat
    18061 accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag gtaagaaaca
    18121 aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg ctcaagcaat
    18181 gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga tgcggtattt
    18241 agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag atcgttatcg
    18301 ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt ctgacccatt
    18361 atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat tcgaacagaa
    18421 aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt tatcgaatgt
    18481 ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg atatggtgtt
    18541 ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt ttttgaatcc
    18601 tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc ttatcgaaag
    18661 tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag ttgaagagtt
    18721 ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa taaaaataag
    18781 ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa tgaaatagaa
    18841 atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa aatcaggaaa
    18901 cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca ttgtcccatg
    18961 gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga aaagatgatc
    19021 actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac ttgcaaacaa
    19081 tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca gatgttccga
    19141 caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat tcgccttttt
    19201 taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa gagctgctta
    19261 aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct ccgaaaatcg
    19321 aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa aagataatca
    19381 aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa gaggctggga
    19441 caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt tcaaattttt
    19501 gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat acgtgtttag
    19561 agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga gattcgttgc
    19621 atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt gaaaccagaa
    19681 gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata ttgttttccg
    19741 ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc cactaatcca
    19801 aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt acgatttact
    19861 cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg aatatctaaa
    19921 agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg attcgggatg
    19981 aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac agatacgacg
    20041 gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat ctgttgttcc
    20101 atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc aagaccgatt
    20161 gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc gatagcagat
    20221 aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt taaaagacta
    20281 tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg attaaaagac
    20341 ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat caaaaaaatc
    20401 agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat cccgtcccaa
    20461 aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc gccagaagag
    20521 tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct aaaatttgat
    20581 tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata actataatga
    20641 aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat gtggagaaaa
    20701 taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt cttatatcaa
    20761 aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag gagaattgac
    20821 agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg gtcagcaatg
    20881 ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct ttcgacctga
    20941 acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa tggtccctga
    21001 aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt ttgtgccgcc
    21061 ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg gtgaaaacat
    21121 tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag taggagcgta
    21181 tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc gtgctgctcc
    21241 taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac ttccaggaac
    21301 agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca cacatacgaa
    21361 aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa aattcatcac
    21421 tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc agttagctga
    21481 agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc tagatcaatt
    21541 tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg ggatttatca
    21601 caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa caaaacggtt
    21661 gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag gctggattga
    21721 agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa aaatcaggaa
    21781 gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa tgcaacaaaa
    21841 gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat tccgctggaa
    21901 agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac accttgctga
    21961 tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac acctgtgaca
    22021 gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt aggcgagaac
    22081 tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc catcatgtta
    22141 taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc gctgtttggt
    22201 gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct gatatcttga
    22261 gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc ttctttgttg
    22321 gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc gatggcattc
    22381 attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa tgcttcttcg
    22441 ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata gataatttct
    22501 gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt gttagcgacg
    22561 cccataatga cgattttttt tccttctaga aatgacataa tgactctcct ttgtttctga
    22621 taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt cttgatttct
    22681 taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta aaaaaagatt
    22741 gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg caattgcctg
    22801 aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg gaattgattc
    22861 ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca ggaaaaaaga
    22921 tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac tttcctgaca
    22981 atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt tcgatactta
    23041 tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc aataaggcca
    23101 aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa atcaccaaaa
    23161 tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa aaagtctgtg
    23221 agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg atatagcaaa
    23281 acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc tagcatacgt
    23341 tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc ttgcagtata
    23401 ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt ttcgatattt
    23461 gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt ataatggcca
    23521 tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg atagtcgtct
    23581 tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg gctgccgtta
    23641 ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata aaggtggttg
    23701 ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc atcgccttta
    23761 ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag tccgttgtag
    23821 taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg ttcgggaaat
    23881 aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc attttcggcg
    23941 acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact ggcagtcgtg
    24001 ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga agtcgtgttg
    24061 tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact taaaataact
    24121 ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt aatgataaca
    24181 aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt aaaagaaaaa
    24241 gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac aaaaacgaaa
    24301 agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc cagaacggtc
    24361 aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt ataaacgggt
    24421 ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag aatttaaaga
    24481 agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca atccagcgtt
    24541 atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga tgggtgccta
    24601 cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga cgcgcaatcc
    24661 ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg acaacatcat
    24721 gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc ctcatcgata
    24781 ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa aatataattt
    24841 gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc ctacgcaaga
    24901 tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag tgaagcattt
    24961 aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa gtgaagaaga
    25021 attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag tagatacgga
    25081 agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata gttatcgagg
    25141 ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca aagataaaga
    25201 tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg acaatgaagg
    25261 gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa acgacggacg
    25321 tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact ttttgaaacc
    25381 atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt gcagaaataa
    25441 tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata ggttatgatt
    25501 tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat ttcgattggt
    25561 gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac actggaaata
    25621 aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg acgcagaatc
    25681 ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc aaaatatata
    25741 tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc acagtcaaac
    25801 gtaatagatt taaagaagtt tttagggaag cgagaaaacc attgattaag ggcagtttca
    25861 atatctaatg ccttacggcc gttgggttta atcgtgataa tgactttaga taatctttca
    25921 actaaagtaa tgacggcact tttatgatga atgccaacga tcgtatcacc ttcaaggtga
    25981 ccaaactcag aattgaaatc aggataatta tcaggtcgat catggattga gcgctgatac
    26041 tgttgttttc cccgtttttc ctgatggcca ttgggttttc ttttaccttt catcggtagt
    26101 gtgtcaatat caaatattcc tttagaaaat aaacgataaa gtgttctcat accgcatgaa
    26161 acagaccttt cttttcgccc gataatgacg tcaggcgtcc aaccaagagt gactttctct
    26221 ttaatgtaat ctacttcatg agcaggtaat tgaatgactt ttctaccaca ttttttctta
    26281 ttttctttat actggtgcca ataatcaaga gcagtcttgc cttgcttgaa cttattgact
    26341 acattataaa tagtttgtat agcacgtccc attcggttag cgatttcaac cggcttatta
    26401 tgttgaagat aatatgattc tatcattgtc agttcgtcta tggtaagatg tttgtagg
//
LOCUS       DADXTG010000029        23560 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.029, whole genome shotgun sequence.
ACCESSION   DADXTG010000029 DADXTG010000000
VERSION     DADXTG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.029"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            183..1949
                     /locus_tag="IWI57_001021"
     CDS             183..1949
                     /locus_tag="IWI57_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBC2449180.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2051..3310
                     /locus_tag="IWI57_001022"
     CDS             2051..3310
                     /locus_tag="IWI57_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBC2449181.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3457..4758
                     /locus_tag="IWI57_001023"
     CDS             3457..4758
                     /locus_tag="IWI57_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2449182.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4761..5612
                     /locus_tag="IWI57_001024"
     CDS             4761..5612
                     /locus_tag="IWI57_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2449183.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5625..6440
                     /locus_tag="IWI57_001025"
     CDS             5625..6440
                     /locus_tag="IWI57_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBC2449184.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6591..7757
                     /locus_tag="IWI57_001026"
     CDS             6591..7757
                     /locus_tag="IWI57_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2449185.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7987..8634
                     /locus_tag="IWI57_001027"
     CDS             7987..8634
                     /locus_tag="IWI57_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBC2449186.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8718..9662
                     /locus_tag="IWI57_001028"
     CDS             8718..9662
                     /locus_tag="IWI57_001028"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBC2449187.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9797..11248
                     /locus_tag="IWI57_001029"
     CDS             9797..11248
                     /locus_tag="IWI57_001029"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBC2449188.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11269..12105
                     /locus_tag="IWI57_001030"
     CDS             11269..12105
                     /locus_tag="IWI57_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBC2449189.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12105..12764
                     /locus_tag="IWI57_001031"
     CDS             12105..12764
                     /locus_tag="IWI57_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBC2449190.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12890..13579
                     /locus_tag="IWI57_001032"
     CDS             12890..13579
                     /locus_tag="IWI57_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449191.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13585..14541
                     /locus_tag="IWI57_001033"
     CDS             13585..14541
                     /locus_tag="IWI57_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBC2449192.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14585..15097
                     /locus_tag="IWI57_001034"
     CDS             14585..15097
                     /locus_tag="IWI57_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449193.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15353..15901)
                     /locus_tag="IWI57_001035"
     CDS             complement(15353..15901)
                     /locus_tag="IWI57_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2449194.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15990..17171)
                     /locus_tag="IWI57_001036"
     CDS             complement(15990..17171)
                     /locus_tag="IWI57_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBC2449195.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17407..18915)
                     /locus_tag="IWI57_001037"
     CDS             complement(17407..18915)
                     /locus_tag="IWI57_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBC2449196.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19074..19265
                     /locus_tag="IWI57_001038"
     CDS             19074..19265
                     /locus_tag="IWI57_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449197.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19468..20385
                     /locus_tag="IWI57_001039"
     CDS             19468..20385
                     /locus_tag="IWI57_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBC2449198.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20378..21451
                     /locus_tag="IWI57_001040"
     CDS             20378..21451
                     /locus_tag="IWI57_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449199.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21599..22117
                     /locus_tag="IWI57_001041"
     CDS             21599..22117
                     /locus_tag="IWI57_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449200.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22098..22490
                     /locus_tag="IWI57_001042"
     CDS             22098..22490
                     /locus_tag="IWI57_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBC2449201.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22566..23501
                     /locus_tag="IWI57_001043"
     CDS             22566..23501
                     /locus_tag="IWI57_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBC2449202.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
BASE COUNT         7763 a         4176 c         4713 g         6908 t
ORIGIN      
        1 actaaatagt acaggaagta gactaaaact tttaaaagtt ttaagtctat ctatttggca
       61 agtttaaaaa ataaaaattc ggtatcaaaa aactgttacc gataacattt ttgaaagcgt
      121 tgtcttttgc ttctctatca cttatagtga tgacgtcata ttaaaacaca aggaggaaat
      181 caatggatac agctgcaatt tatcatcgac cggaaagtga atatgcattt ctatatacga
      241 atacaagatt tcgtattcgg cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc
      301 tatgtggaga tccatatacc ataacaactg aaaaatggta tcaaaaacaa cgaccaatga
      361 aaaaatgtct aagtaccaat gttcatgatt attgggaaat tgaagtgacg agcgaaacaa
      421 gtcgcttaca gtatgcgttc catgttgttg gggaagataa cacggactgt ttttacggtg
      481 atcaaggaat ttttccttat cgagaaaatg tgattactga accaaatttt tattttcgaa
      541 taccttattt tcatcaaatc gatcgattca ctattccaga atgggtaaaa gaaacagtat
      601 ggtatcaaat ttttcccgaa agatttgcaa acggggacaa agcaaatgac cccgaacaca
      661 ctttaccttg gggaagtaaa gatccagacc gagaagactt tttcggggga gatttgcaag
      721 gtgtgatgga tcatttggat tacttgacgg accttggcat caatggcatc tatttttgtc
      781 ccatatttaa agccacatcc aatcataaat acgatacgat tgattattat gaggtggatc
      841 ccgcttttgg cgataaaaaa cttttaaaaa acttgattga tgaagcacat aaaagaggga
      901 tacgtatcat gttcgatgct gtattcaatc atatgggtgt ccattcacca cagtggcaag
      961 acgttctcaa aaacggagaa aaatcgatct ataaagactg gttccacatc cgttttttcc
     1021 cagtagattc ttatcagatg actgacttac cagaaactgc tgaaaatatc ccatatgata
     1081 cttttgcttt tactccattc atgccgaaac taaatacagc aaatccagaa gtgcaaaaat
     1141 atttgctgga tatcgcaact tattggataa aagaattcga tattgatgga tggcgtcttg
     1201 atgttgctaa tgaagtcgat catcattttt ggaaaaaatt cagagaagca gttacagaaa
     1261 tcaaaccaga catctatatc cttggagaga tttggcattc ctctcaagct tggctgcaag
     1321 gggatgaatt ccatgcagtg atgaattatg catttacaga ttcgattaaa gattattttg
     1381 caaaaaagaa aatcacagct tcacaaatgg tcagtggaat gaatcatcag caaatgcttt
     1441 acagagatca ggtgaacgaa ggaacattca acttattaga ttctcatgat actgccagga
     1501 tcttgaccct ttgccaaggt aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc
     1561 ttcaaaaagg gtctccttgt atctattacg ggacagaaat agggatgact ggtgaagatg
     1621 acccggattg ccgcaaatgt atgatatggg agaaggagga acaagatcta gagctattcg
     1681 gttttataaa agaacttgtt tctctaagga aacagttgtc taagattatt tctgaaggtt
     1741 ctactcagtg gctaatagta aatgatcgtg aagataagct ctatttcaca cggaaactag
     1801 agggacagat catctatgtt tactttaatc agtcaaaaga accttgggta gtagaacagg
     1861 aaaatgaggt gattcttagt caaaattgtc agttgttaga agacgggaaa gcagagatac
     1921 gacaatacgg atttttgatc atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt
     1981 tgcaaatgaa gatttgtgcc cattataata agggtgtaac agcacgttga atacaaagga
     2041 ggaaatgaag atgaaaaaaa gaagtgtgaa acagttgggt ttagggttaa tctctttagg
     2101 aatcttaggc ggattagcgg cttgtggcaa tggtaactct tcagcagaag gggaagccga
     2161 tgaaggaaaa acgttaacag tttccgttga ttctggatat aaagattata taaacgaaat
     2221 aaaaaacact ttcgaaaaag aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt
     2281 cgatcagttg gaatcattag cgttagatgg gcctgctgga aagggacctg acgttatgat
     2341 ggcagcatat gaccggatcg gtgcgttagg acagcaagga catctggcgg aagtaaaact
     2401 gggaaatgaa gcagcttacg atgaaacaga caaagcccaa gtgacttatg atggaaaaat
     2461 ctacggagaa cctgcagtaa tcgagaccct tgttttgtac tataacaaag atttagtcga
     2521 tacagcacca tcaacgttta aagatttgga gaatctatcc aaagacagtc gttttgcttt
     2581 tgagtcagaa gcaggaaaaa atacaggatt tttagctaaa tggactgact tttattattc
     2641 ctatggttta attgcaggtt acggtggcta tgtatttggt gatgatggca caaatccttc
     2701 agatatcggt ttgaataatg caggtgctgt agaaggaatc tcttatgcaa cagattggtt
     2761 caaaaatgtt tggccaaagg gaatgcaaga cataaaaagt gcaggagact tcgccagcca
     2821 gcaattcatg tccaataaaa ctgcagcaat cattgacggt ccatggcaag cgcaaacata
     2881 caaagaaaac aatatcaatt atggtgtagc caaaattcct actctaaata atggacagcc
     2941 ttatcaaccg tttggcggag gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga
     3001 cctctctcaa aaatggcttg attatgtaac gaatcaggaa aatcaagaaa aattctttga
     3061 aatggtaaat gaaatccctg caaaccaaca agctagggaa acagcaaagg ctaaaaacga
     3121 tgaattaaca acagctgtga ttgaacaata tgaaacagct caagcgatgc caaacatccc
     3181 agaaatgggc gaagtttgga caggtgcaga aaacttgatg ttcgatgcag catcaggttc
     3241 aaaaacacca aaagaatccg cagatgaagc agtaaaaaca atcagtgaag ccatagaaca
     3301 aaaatactga ttaaaatgat aaccgtccat ttttataaaa aatggaacag gtgcttcaga
     3361 taaaagtggc aagagcgaga tggaaaatta tcttgctctt gttgccatta tcttaaaagc
     3421 agtttgcggt ttagcaagtt tagagggggc attcagatgc agcaagataa aacaagcaat
     3481 atcaacgaaa aaaaaatcac gctttgttca cttattccag ggatggggca gtttgtaaat
     3541 ggccaagctt ttaaaggtat cgtttttttc gccatattcg tcctattcat tggtcagatg
     3601 cttctgggcg gatttcatgc attggtagga ttggttactt tgggaagtat accaatggaa
     3661 gatcattcat tatttatttt gattgaaggc actttgcaat tcatcattac aatcattttc
     3721 ttgatttttt acggtttgaa tcttttagac gcttatcgag tagcgaagat gcgtcaagag
     3781 ggcaaaaaaa tcaatcggac gatgaaggaa gtcatacata atataggtga tgaaggtttt
     3841 ccttatctat tgacacttcc cgcttatctt ctcatgattt ttacgatcat tttcccagtc
     3901 ctagttacat tgttcactgc atttactaat tatgacttca atcatattcc accagccagt
     3961 ttaattgatt gggtaggggc taaaaacttt tttagtatct tctttttaag ctcatatcgt
     4021 aatacttttt ttgcagtctt ttcgtggacc gtgatttgga ctttatgtgc aacaactttg
     4081 caaatcaccc ttggtattct cacagcggtt gttgctcacc agaaatttat taaattcaaa
     4141 cgtattttcg gtatcatttt cttattacca tgggcggtcc cagcttttat tactatcatg
     4201 agtttttcga atatattcaa cgatagcgta ggtgccatca atactcaagt gattcctttc
     4261 ataaaccagc ttccttttgt agatatcagt gcaatctctt ggaaaacgga cccgaactgg
     4321 acgaaagtag caattatctt agtgcagggc tggttaggat ttccctatat ctatgtgatg
     4381 gttacaggaa tcttacaatc gatttcagaa gacttatatg aagcagcaaa aatcgacgga
     4441 gcaaatgcat ggcagcgctt ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg
     4501 ccaacattta ttacacagta tacggggaat ttcaataatt tctccatgat ctatctgttt
     4561 aacaatggcg gtcccggaag cgtaggcgga ggagctggtt ctactgatat tcttatttcc
     4621 tggatctata aacttacgac tggaacttct ccgcagtatt ctatggcttc agcaatcacg
     4681 ttgattatct ccgtcatcgt tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg
     4741 ttcaatatgg gggattaaca atgaaaaaat ttcattcgag tgctcgattc caaaaacgac
     4801 tttctcactt ttttacttac ttttttcttt taatactgtc agtcattatc atctatccgc
     4861 ttttgatcac agcaagtact gccttcaagt caggaaatat tgcagctttt agtttagatc
     4921 tttcaagtga ctggacttta ggaaacttta ctcggctatt cagtgaaaca ctttacggga
     4981 cttggtataa aaatacattg atcatttctg tcctaacaat gatcgtacaa gtgactgtag
     5041 taactttggc gggctatact tatagtcgct atcgttttat cggccgcaaa tcaagtttga
     5101 tgttcttttt agtgattcag atggtaccga ctatggcagc attgactgcc ttttatgtca
     5161 tggctttctt attagaagcg ctcgataagt actggttctt gacaatgatc tatataggcg
     5221 gtggaattcc aatgaatact tggctgatga aaggatactt tgacactgta ccaatagatt
     5281 tagatgaatc tgctaaactt gatggggcag ggcatttcag gatttttttc cagattgtat
     5341 taccgcttgt caaaccgatg ctcgccgttc aagcattatg ggcttttatg ggaccttttg
     5401 gggatttcat gttagctaag ttcctgcttc gaacacctga aaatcagact gttgctgtcg
     5461 gcttacaaac atttatttct gatgtgacaa accaaagagt ctctcttttt gctgctgggg
     5521 caattttgat tgcactaccg atttgtctat tattcttctt tttacaaaag aactttgtta
     5581 ccggcctgac atctggcgga acaaaaggat aggagcagag tatcatgcaa acatttccgt
     5641 taaactattt ttccgcatta cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat
     5701 catggcctaa atttttcttg atatttgtat ttttagtttc cttgatggtc atgcctgtga
     5761 ctctttttta tgccaatcag atccaagcga ttccattaga acaatttcta tccgttcatt
     5821 cgctgatcga tgaacaagga acccaaaaat tttctgaact tgaattatct gaaactgggt
     5881 tacaagcatc gcaacaaacg atagctgtga cccctgaaat tttggtaggt gtgtctttat
     5941 ctgagaaaca acaatccgat catggaactt ttattgattt tgagaaggaa caatgggtga
     6001 tccaacaaaa ggataaatct ggtatacgcc gctatacgat gaattatagc ccatctttcc
     6061 aaccagattc tgtacgtacg cccgaagatt tccaacgatt tttagaaaga gaattttatg
     6121 ccagtaatcg tccaacaatc attttatctt atagtttaag tcttggtctt gtcctatttg
     6181 tcatgacgag tctcatttta ttcggggctt cctttttctt gtggatgaca agaaaaagcc
     6241 aactttcatc cattcataca ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa
     6301 taggttccat ggcagcagca gttgtagggt ttatccactt tgactttatc ttgatgctaa
     6361 gtgtccaaac aacgataact gtcttgctgc ttttatgggt ttttgtcaaa acaagattca
     6421 aagataagcg tgtcgaatga caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa
     6481 tgaaaaataa agtagttttt gaaaaatttc tgaaaaaatt aaacttctta gttgattctt
     6541 ttctttttta gtattaacgt aatagggtag tttttttaag gaggaaaatc atgttagaga
     6601 aaactttata caatcaatta ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt
     6661 gggacggtac aacgaaacaa tacggagata cggacactcc tccccaaatc aaaatcaata
     6721 tccatgaaga aatcccaatg aaagagatta caagtaacgc atctttggca ctaggagaag
     6781 cctatatgga tcatcgaatc gagatcgagg gaagtatcca agcattgatc aacgatgctt
     6841 atcagcaagc aaacagcttt ttacgaggaa aagattatct aaaatggctg ccaaagaaag
     6901 aaaaacatac gaaacaaaaa aacaaagaag atatccatgc gcattatgac ttaggtaacg
     6961 acttttataa actttggcta gatccaactt tgacttattc ttgtgcttat ttcaatacac
     7021 ctgaagatac gttggaacaa gcacaaatca ataaagtcca tcatatctta gacaaactct
     7081 ttatcaaaga aggcgaaacg ttgcttgata tcggttgtgg ctggggaaca ttgatgttca
     7141 ctgctaccaa agagtataat gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg
     7201 atttgattac caaaagaata aaagaagaac atttagaaga taaatgccgt gtattattaa
     7261 tggattaccg cgaactaaaa ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg
     7321 aacatgtagg ttctgaaaat cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac
     7381 caaaaggaac agctttgatc catggaatca gccgacaaca aggcggtgcg acaaatgctt
     7441 ggatcaataa atatattttc cctggcggtt atgtccctgg tgttactgaa ttagttgata
     7501 atattacaaa aaacgatttg caactgattg atttggaaag cttacgtcgt gactaccaat
     7561 tgacattaga acattggaca aagaattttc atcaagtcca agatcaagtc acgaaagaaa
     7621 tgggtgattc cttctatcga atgtgggatc tatatttaca agcttgtgca gcttcatttg
     7681 aatcaagtaa tatcgatgtg attcaatatt tgcttgttca acctggaaac aatgacatcc
     7741 caatgcatcg tgcataattg aggcaaaagt actgtttaaa gactatttaa aaagaagcta
     7801 agttttatga tgctccagaa gtgaactttt ttgtctaact tctaggattg tctctaaagc
     7861 ttagtttctt tttctttata ttttcataat aaaagtttgg ttctttaatt tttaaacatg
     7921 acagaaaatt ttttcttcgt tatacttgta atagaatact attttataac aaaggggatc
     7981 gaatcaatga aaaaaactgt tatttatact agtttatttg cagctttgtt tttagctgct
     8041 tgtcaatcaa ataccaatca aggcacatct gggaatacgt cttccagtag cgaacagcgc
     8101 tcttcacagg tatcggactc ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa
     8161 agttctagtg aagcaagtac tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa
     8221 ttgttccaag ataaataccc agatactgca attactagtt tggaattaga tagtgattgg
     8281 ggatcttact tctacaagat agaaggtgtc gatgatcaaa aagaatacga agcaagaatt
     8341 gatgctcaaa atgaaaaaat ggagacagag aatccagaaa ctctagatcg agatgaacaa
     8401 aatggtcaaa agaaaaaaga agatggtctg gatgttgata aattgatcac gattgaggaa
     8461 gcagcaaaaa tcgccgtcga caaagtagga agcggtacgg caacagattg ggatttggat
     8521 aaagatttag gtactactta ttgggaagtc aaagtaaaag acggcaatca aagtacatca
     8581 gtcaagatca atagccaaac aggcgaagta ctgtcaactg aacaagatga ttaagaattt
     8641 cttcggaaaa caagagttga tttttctttt agtcatgata cactagacaa aataacttgt
     8701 ttgaaggagc gatttacttg agtttagaaa cgacctttgc caatcatatc tttgccaatc
     8761 ctttgatgaa tgcatctggt gtccactgca tgactaccca ggaattagat gaactggctc
     8821 attcagaagc aggagcattt atcactaaga gctgtacgat caatgaacga aaaggaaatc
     8881 ccgaaccacg ctattttgat gttcctttag ggagtatcaa ttctatggga cttccaaact
     8941 taggtttttc ttattatcta gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa
     9001 atcaaccgtt atttttttcc attgccggga tgagtgtcca ggaaaacttg gaaatgcttg
     9061 agaaaataga aaaaagtggg tttaatggga tcactgaatt aaatttatct tgtcccaatg
     9121 tgcctggaaa accacaacta gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag
     9181 tattcagcat cttttctaag ccgctaggaa tcaaattgcc tccttatttt gattttgctc
     9241 attttgatca gatggctgat atcttgaatc agtttccatt gacctatgtc aatgctatca
     9301 acagtgtggg caatggtttg tatatcgata cagaacaaga agcagtagtc atcaaaccaa
     9361 aagaagggtt cggcggcatt ggcggagagt atatcaagcc gactgctttg gcgaatgttc
     9421 gcgcttttta tacacgtttg aaaccagaaa tccaaatcat tggtacaggt gggattcgga
     9481 caggacaaga tgcatttgaa catctgctat gtggcgcaag catgttacag attggtaccg
     9541 agcttcataa agaagggccg gaaatttttt ctagaatcat aaaagaatta acgcagatta
     9601 tgtcagaaaa aggatatacg tcaattgacg aatttaaagg caaattacgc accatttcat
     9661 agcaaagaga cgaaggtcat gattgatctt cgtctttttt tataaaaaac actcgtctgt
     9721 tcattcctgt gcaagcgtac tcattgtatg ttagacgaac ttgtgggaaa ataacagagg
     9781 caaaaaggag gataatatga atgatgcaac agaattagca aaagagctta aagaaaaaga
     9841 atttagtttt caagaactag ttacggatat acagaaaaaa ataaagaagc aaaatcatga
     9901 attgaatgcg tttgtcgcgc ttgaaccatt cgagagtaat tcccacgagc tattgattcc
     9961 ccagtcagaa aaagggccat tggctggtat cccatttcct ttaaaaatgc tcggtcaaga
    10021 aaaaaaggga tggctagcta cttcaggatc tcgtttattt gaaacacacc gagcttctca
    10081 tacatctaat tatgtgcggc aagcagaagc gataggtcta gttccttttg gacaaaccaa
    10141 tgcgccagag tttggtttca aaaatattac agacccagtc atctatggcc cagcaagaaa
    10201 cccttggaac cttgaatgta caccgggcgg atcaagtgga ggagctgctg ctgcagtttc
    10261 atcaggaatc gttcctttag ctggtgctag tgatggcggt ggttctatta gaatacccgc
    10321 ttcgttttgc ggattgattg ggctgaaacc ttcgcgaggg acgatgccag ttggtccata
    10381 tgcttggaga ggatggcaag gagctgctat tgatttcggc ttgacagttt ctatgagaga
    10441 cacggaggct ttattttatg ggatgagaag tatccattcc ggtgcgccat atcaagtatc
    10501 gccagttgag tggcaaacac atccaagaaa aaaacggttg aagatagctt tttgtgttga
    10561 ttcaccgatc cactcacaga ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga
    10621 tttccttgga aaagaagggc atgatttgac agagattcct tacccactag atggtcgtaa
    10681 actgatcacg tcttattatc agatgaatgg cgcagaaaca gcagcaatga tccgctcgat
    10741 tgaaacaggc atccagcgag ctgtcagaaa agaagaactt gaacccattt cttggacttt
    10801 gttccaatat ggtcagaagc tctctgccgc tacttacatc gattctctgc atgtatggga
    10861 tgaagcagca attgtcatgg aaaacctaca tcaagaatac gatctattcc tttcaccaac
    10921 gactgcattt accgccccta aaatcagcga agatttgcaa agtgatgcga taagagcaca
    10981 gatggaacac atcagtgatt gttctgaagt agaaggttta gacgttgttt cttccatgtt
    11041 tgaggagagc ttgaagatca caccttatac acagttggct aatctgactg gacaaccagc
    11101 aatcagcttg cctactcatt tgacaaaaga aaacatgcct ctaggaatcc aatttatggc
    11161 tgccagagga agagaagatt tgctatttca aatcggctat ctatttgaag caaatcatca
    11221 gttccattta ccaaatccat ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa
    11281 agattgtttg ctctaataac tagtgccttt tttattttaa gcgcttgcgg tacagctgcc
    11341 gctaatcagg aagataacag ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg
    11401 acttctggca cactgtttcc cgcatcttat tataatgatc aaaatgaatt agttggttat
    11461 gatgtagatt tggcaaaaga ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa
    11521 tacaacgtgg atggacaaat cacttctgtt tcaaaaagag aatcagattt tgctgcgaac
    11581 gattttggta tcacaaaaga aagagaaaaa aaattctccc tttcagagac tttcaaatat
    11641 tcatttgaca gtatgatcgt acgaaaaaaa gatgattcag gaatccactc acttgaagat
    11701 cttaaaggga aaaaagcagc tggcgaaccc aatactagct atatgcgttt agcggaaaaa
    11761 cacggagcta aattagtcac atatgacaat gccacgaatg atcagtattt aagcgatgta
    11821 gcaaatggtc gaacagatgt tattctaaat gattattatc tgcagaaaat ggccgtatct
    11881 gctttaccag atatccctgt aaaaatactg gagaacgttt atttcaatga aaacgaaagt
    11941 ggttttttat tgaaagaagg aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa
    12001 gagatgaaag aagatgggac gacaaagaaa atatctgaaa aatattttca aactgatgtt
    12061 tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg agtgatgtac tttcctctaa
    12121 ttgatttttc cctcatgtgg acttcacttc ctttcgtact gcaaggcttg gtatacacat
    12181 tagggattgg cttcgtgtcg tttcttttag gaaatctggt agggttgctt ttgacagttt
    12241 taggtctttt ggattggctg cctctgaatg tatttattcg tttttattta tcgttcttta
    12301 gaggcattcc cgcactagtc ctgctgtttc tgttatattt tgggttacct tatcaattaa
    12361 gtgccttaac ggcttcagtc atctgcttta cgattaccag cagtgcattt attggtgaaa
    12421 tctatcgagg atcactagca ggcgtttctt caggtcagtg ggacgctggt ttagcttctg
    12481 gtttttcttt ctttggggta ttgaaatata tcgttttgcc gcaagctttt cgaatctcag
    12541 taccggcact cagtaacgta gctatggatc tagtgaaagg tacttcgtta gcagcaacaa
    12601 tcacagtgcc agaaatattt caaaaagcaa agatcatagg cggtcggaca ttcgattatt
    12661 tttctctcta tattttagtt gcactgattt attgggtcat ttgtatcggg atcgaaaaag
    12721 tgcaacagta tttagaaaaa cgttctatgc atcagtcaat ctgaaaaata gaaatggtgc
    12781 tagcgtggct ggcaccattt ctattttttg tttcttgtat attctgccta gtattttaaa
    12841 accatttcat gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca tgaagacccc
    12901 gctaaccatc gaaatagaat cctcgttata ccaatattgc atcgagcaag gcggcttggt
    12961 tgtagaagaa gtcacgatgc ctgatgacca aggaattgta gatacgctta gctgcttgac
    13021 aaaatttgat ggaagccgag aatggcgatg ctatgagctg aaagtgacaa aagcagattt
    13081 tcgctctaca gccaaactgt catttgttgg acattataat tattttgttt taccaagtaa
    13141 actatatgaa gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc
    13201 gtacacggtt gaagaggaga tgcctgcctc tggtacattt ctgattgcga aaaaagcagc
    13261 aaagaaagag cttgctgtac cggaagaagc tttgacaaac cgcttcatgg cttctttatt
    13321 cagggaagta agaaaagcaa agcaaatgga gcgaggcgta aaatattttc caacagatca
    13381 attgtacaaa gaattgaaaa gacgttcaga aaatcaagat ccattttcta acgaacgata
    13441 ttatgaacgg tttttagagg aaatagctag tcaggcaatc aatgagttgc aagaagaagt
    13501 tatcgcgctt cagcatgatt atgaatattt gaggcaacaa cagcagatcc gtcggttgcc
    13561 tacagagcca ttggagtgaa aaatatgtac tatccttatt taagaggaaa acaatttgac
    13621 ctattagcat taaaagaagc actgtcgcga ggattgctct ccaataaaat ccaaccagtg
    13681 atcgaaccag tacgtgattc cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag
    13741 caccatcgaa tcgctgtgat ccaaaatccc caagtaggac aattcaaatt attcgatcag
    13801 catgtgcaca gttggacagt caaagaaaat agctctgtcg tccctgcaca gatcttaacg
    13861 ccagaaaatc tcagtgaagt actggattca cctccagcgt ttcttgtttt cgatggacag
    13921 cactacccaa aagatacaga ggtgtggaaa cagttggcag gagttgattc gaaatttttg
    13981 ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac
    14041 tcttttcaaa cgagaaagca tgttgaaagt tatgcagata aaacagatga cttttttagt
    14101 gatgactatc tttttttcca cgcagacgga tatattggat tttctgattt cacgatcgaa
    14161 ggtagccgtt atttcgataa agggttccct agccgagcgc ttgctatcca tctaacgtat
    14221 attgatgctt acggcaatat acgcgtgaaa cattttgtct ctgacagtaa cgactctgct
    14281 aaagaccaag cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt
    14341 caccatcatc aactcttgat tacttcagga atgattgaat tattggctct ttaccagcaa
    14401 caaaaattcc ctggactggg agtgttgaaa aagtggtcac ttatgcatca tttggaactt
    14461 attagtcacc tgctagatca tccaaaagat tggcttaaat attattcaaa agttcaggaa
    14521 ctatatgaag tgatccaatg attagataat gtaaatgaaa aaggaataga aaagaggaaa
    14581 ctggatgtat attcggcaaa caaggaaaga agaagtagaa gaagtggtag aaatcattaa
    14641 tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc
    14701 aacaaaagaa acgattttga acgatattga aagaaaagaa agctatgtat taatagtgga
    14761 agatcaaatt gctggaacag ctgcattagt ctcaagcgtg gatcccgttt acacaaaaat
    14821 agatggtagt tggaaagaat caactggtcc ttatttatcg attcatcgtg tcgctttagc
    14881 agaaagtact cgaggaaaaa aactagcaaa accgttctta gaacatttgt tgacgattgc
    14941 agccagcaaa aatatccaag atatccgtat cgatacttat ccagctaatg aaccgatgga
    15001 aaagaccatc tattctgctg gattcactta tcaagggatg attgaatttc cttttgaaca
    15061 cggcgaaaga aaagcatatc aaataatcat caattgacta taaaatcatc tgagcaaaga
    15121 taaagacatt aacactactg aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa
    15181 gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa
    15241 ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac
    15301 atagcttttg tcacgacctc tcggcttatc ctacgagtat tattcgatac atttatatca
    15361 ataatccttt ttttatttta tctgcagctt cttggaacgc tccatcgttt gacacgacta
    15421 aaagtgaggg gatagctttc aaacgtttca actgtgctaa atcttctaca taaaccgcca
    15481 gtctggctga aatctgttca gatgaatctc cacgtttttt caggcgcgat tgcaatgttt
    15541 ctttagagat cgtaataaag ataggacgga ctaccgtccc gaaatactca tataaatgcc
    15601 aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc tttttaattt
    15661 ctgctttact ggaaccatat ttatgttgag catattggtc ccactctgca aactcatttc
    15721 ccttgatcat ctgattgaat gtattgtcag aaacaaaata ataatcctct tgatcttttt
    15781 ccccaggtct tggtgatcga ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag
    15841 cttctgcgag tgaagttttt ccagaaccag aaggaccgat aaatacatag caataattca
    15901 ttgtcatttc tcctttgatg attgaacaag tgaaaaagct ttggaaaata tttccaaagc
    15961 ttttaaaagt aactttgctt ttttgtatat tataccagca agccagtcac aatagcactt
    16021 aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc gacaagattt
    16081 cctttttctt catttaatcc cttcatcgca ccagaaatga tcccgataga agagaagttg
    16141 gcaaatgaaa cgaggaagac agatataatt gctgttgtac gctcagtaaa atggaaatta
    16201 ccatttgcta attctgtcat agcaacaaat tcatttgtaa ccagtttagt cgccatgata
    16261 ctaccggcat ccactgcttc tttccatggg attccgctga tgaaggcaag aggggcaaag
    16321 atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat agcattgatc
    16381 atggcaatca aagcaacaaa accgataagc atagcagcaa ccgtgatcgc cacatggaaa
    16441 ccatcaagaa tgtattctcc caacatttgg aaaaatgtct gttctttgtt ttcttcaatg
    16501 atcagctcat cttctttttc ttctaataca tagggattta caattgaagc cagaatataa
    16561 ccgccaaata agttcaaaac aagggcagtg atcacatatt tagaatcgat cagtgccata
    16621 taagatccga cgatcgacat agaaactgta gacatagccg atacactcat ggtgaacaaa
    16681 cgtttctcag ataggaaggg taattccttt tttatggaaa taaaaacttc cgattgtcct
    16741 aaaatagcag aagctactcc attataagat tccaatttgc ccatcccgtt gattttactt
    16801 aatcctagac caacagcttt cataaataat ggaagaatct tgatatagcg tagaataccg
    16861 ataatagcag aaataaatac aattggcatc aatacggaaa gaaagaaagg tgcacttcct
    16921 ttatttgcta caccggcaaa aacaaagtca acaccttgcc cagcaaaagc aagaagatga
    16981 tcaaacattt ttgccaatac agaaacaacc gctgtaccaa aagttgtacg taataatacg
    17041 aaaccaagaa caagctgtaa aacgaacatc acaattagtg gtttcaattt gattgctttt
    17101 ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa ataaaccaat aatacctaat
    17161 aaataactca aataatcact cctattccaa attccgtttt attttacctg ttttgcaaat
    17221 aaaatactaa tctttagtga aattttcatt acttttttag agtgaaacgc attcattttc
    17281 cattagaaaa taaagcgaac aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc
    17341 tataaaaaaa cgaaactatc gattaagcta atagtttcgt tttcaacatt tataagtaaa
    17401 ctttttctat tggtaatata gattttcgga agggtagaca ggtgcgcagg taatatcgat
    17461 tcctagtttt ttcaatgttt gttcatcatt tttattgacc atcacagtag agtgggcttg
    17521 tgctccctca agttcagcta gtttatcata agcaacttga gcgattggat tcgttactgc
    17581 actgattgat aaagcgatca agacttcatt gatgcttagt gcatccaaag aggaatgaag
    17641 tgtctttgat ttcaatcctt gtattgtttc aagaactaat ggtgacaata aaggaatgtc
    17701 atctgcgata tgggctaaat gttttactgc atttaagata actgccgctg acgcatccat
    17761 caatgaactt gtacgcccgg taatgatgac ttcatctggt aattcgattg caatcacagc
    17821 agaaggatct tttgtaggac tttgcgcttg aatcttatct gcatagtcat gggcagggac
    17881 taccacgttt cggtcttcag gacgtaaacc gacttcttcc ataatcagtt tgattcgatt
    17941 aactgcttct tcgtcgatca atcctttttt gaagtcgcat tctgtttgga aataacgacg
    18001 gatgatttct tgtttagagg cttcttggac aacggtatca tccacgatcc caaatcctac
    18061 acgattcaca cccatgtcag taggagattg ataaacagat tctttaccag taattttttc
    18121 aataatacgt ttgattacag gaaatgtctc aacatcccga ttatagttca cagctacttt
    18181 attgtaggca tcaaagtgga aggaatcgat catattgaca tcttttagat cgactgtggc
    18241 tgcttcataa gcaatgttca gcggatgttt caacggaaca ttccatactg ggaaagtttc
    18301 gaacttggag taaccagctg catgtccttt ttggctttca tgatacagtt ggttcaagga
    18361 agtagcgagc tttccacttc ctgggccagg agctgtcact acaacgattg gtttagttgt
    18421 ttcaatatac tcattttttc caaaaccatc ttctgagacg attttttcta cgttgactgg
    18481 atagtcttcg atttctgagt gtttatagac cttgatgttt cgtctttcta gtttattgat
    18541 aaagactttc gtggcaggct gtccactata gcgagtgata acgacactgt tgattaataa
    18601 gccataacca cgtaattcat cgattaaccg caagatatcc atatcataag taatcccata
    18661 atcaccacgg atcttattac gttcaatatc tccagcatag acgcaaatca gtatttcagc
    18721 ttgatctttc aatttttgca gcaatttgat tttcgcatct tcttcaaatc ctggaagtac
    18781 acgtttcgca tgcttgtcgt caactagctt cccaccaaat tctaaatata atttgtcata
    18841 gtggttcact cgttccaaaa tatacgctga ctgctcttca atatattttt ctgaatcaaa
    18901 accaattttt ttcactaggt tccctcctta agtactaaag cattttttca tttttttgag
    18961 gattcgtcaa ctggaagagc gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac
    19021 ttgtctagat agttattcat tggtatgata gtttttgata aggaaggtgc tacatgaaaa
    19081 aaagacagaa aaagaaaaac gcatataaac actatatccg ttcgattttt accggttatg
    19141 aaagaatgct tgaagatccc gaattggaac aattgacgtt tacttatcta aacgaagaaa
    19201 cgcagctaac tcgtgatgag catcagcgca ttcatttcac aacaagagat ctgcctagta
    19261 aataaaaaat aagataaagg tcatcggatt cgattctgtc ctatcagatg gaaagactag
    19321 ttgatcgaaa aaaaggctgt accaaatttt ggtgcagcct gttttatgga gcattgcccg
    19381 ctctttttta taacaatttt ttacagaaaa tggtactatt gaagtattaa taagtgaaaa
    19441 acagatgagt aagaggaggc aagtagtatg aaagagccag atacaaatgc aaattatgag
    19501 ccagacgaac tagaggagac gaatgcggat caggatacag gacaagaatc gtcttctttt
    19561 tacgatatca aagaaaaagc aagcatgaat ctttcccaat tattagaaga cttacaggct
    19621 tttgaaaaag ctgtccgcga agaagatatc cctgagatct accgtttata caatggccga
    19681 ttgaatgaag aattaaaaag aagctcaaat gaaaatcacg agatcgatca attgctgatg
    19741 cgtaagatcc atgatcgttt tttacaggaa tttcctttta tggaacaagt agaaaacatc
    19801 tcgccaacga tgagctatta caaaatcggc acttattacc atgatcggcc cacggtcggt
    19861 attgatgcaa gtctgccaga aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa
    19921 tattcccaac cggagtcaac ggactatgac aaaaaaatca atgagctaga cgcaaaggtg
    19981 atcactgctc agacagaaat tgaacgttta gatgaacaaa taaaagaaat caatcgacaa
    20041 atgactgatt tgaacgacaa taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt
    20101 caagaattag agaagaagaa gcaagtacta tctaatgaaa aattaggctg gcttccttat
    20161 attgagacgc ctgaaacaat ccagcagcaa aaagaagcat tgaagcaaga agcaagagca
    20221 gaccagcttc gtgcagccat cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt
    20281 agcaaagagg gattcggtca agcaatccat gaattcttga tgaattatct tggcaatgag
    20341 aatcctaata atcaatctaa tgaaggaggt tccgagtatg aatgaacacg atgaagcaga
    20401 atggacaaaa aatgaagaac aaacagaaga tcatgaagaa acgaacactt cattggaaga
    20461 acaaacaagt aatcctctgg atgacacaga agaaatccct atgctagagg aaacaaaaat
    20521 acacgaaaaa gaaaaagtaa ctccgacgaa tacgaatact gcagaaaaca cccatgctta
    20581 cgaaataact gaaaatcccg ggtatttaga aggaaaagag caagatcatc aagatatcgt
    20641 cagtttgatt gaaacagcgg aagcagaatt agcggattta cgtatgagaa aagatttgat
    20701 cgaatcagat tttccacaat tattagaatc atatgatcgt tttatattga atgaagaacc
    20761 aatctccacg atcggaaaga caagcctgat cgaatacttc acgtatgaat tactgaaacc
    20821 gttgaatgat attggtctgg aacggtcaaa ttcagcttat gacacatgga aaacaagaca
    20881 tttttcgatc aattatacgt tgaatggaca aaacgaatta gtcttttcct ttgtattacc
    20941 gaccatcgat caacgatacc aagtggaatc gatgagtctc ctaaacgtat caccagagac
    21001 aatggaaatc aatgtccaag atgaccgggt gctttctttg atccgacact ggtcagtgga
    21061 tcgagtattc tctgcagggc agatcacgat attcaatcac aaattgaatc aattattagc
    21121 ccatgcaaga acacttggtt ttaccgttaa tcagacatta ctagacaata cgaagccact
    21181 tcgtttgaat cttcagagcg aatttgaatt gacgaatgaa gtattggatg atatttttat
    21241 cgtggcaatg aaaaatccat cctacgatat ggaaaagatc aacgaaacta cttatcaagt
    21301 acgattagat aaaggacagt ctttaacgat aacaacaaac gaaaaagatc aaacagtctt
    21361 gtcgatctca tctggagact acccacgatc tgtcattgat ttctttatca attacgaatt
    21421 cttggtccca ttaatggtga gaaagtcctg attggataag ttggattacc agatgtcatt
    21481 cagataattc tgttccaaag gacttgcggc agttatccag aataattgtc acaagtcttt
    21541 tctgtatgta ctaaatggac gtgaataact tgctgatgaa ggagggattt cattaggaat
    21601 gtccacaaat aaaatagcac agcttagctt attgtctgct gcttgtgtag caggtaggat
    21661 cgcttttcaa tttattccga atttccagcc aatgactgct atttttttat ttatagtact
    21721 ttacttaagc ttaaaagatg cgttaattgt catgagtctt tctattgcga tcagcagttt
    21781 ttattttggt gttggaccat gggtcatcgg ccaatggatc agctatactg ttgttctgag
    21841 tttgttttca atcgtctgct tgagaaaaac agtgcaaagg aatctatggt taaagggagt
    21901 ctgttttttt ctggcgggca tggtttacgg aataggtatg accgtttttg atacgctgtt
    21961 atatcgtttg cctcaacctt ggatctatta ccttcaaggg gtgtcgtttg atttgatgca
    22021 cagtataggc aatgttgtgt tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa
    22081 tcattcagga ggaaaaaatg aaaaaaataa tttatagttt gcttgcaatg agtgtcagta
    22141 tactcatttt gacaagttgc agtacaacct atacacaatc ggcttccaac caaaaggagg
    22201 aagtaaaaac atcgcaaaaa gcaacaatca cgttacaaga aaatggtcaa aacttttcgg
    22261 aaaaagaagt gttcttcaaa aaaggcgatg atttactgag tattttgaaa cgaaattttg
    22321 aagtcaaaga agataacggc ttcatcactt cgcttgaagg gcactcacag gatgagaaaa
    22381 atcaactcta ttggatgttt actgtggacg ggaaaagtgc tactactggt gccaaagaga
    22441 tcaaattgaa tgatggacaa tcggtcattt tcaatttgtc taaactataa gtttcataaa
    22501 aaactgttaa agggcgaata ttgccataat tcagtgagtt cagttgatga aagaaggaaa
    22561 ataacatgga aaattttaaa aaaagcggaa tgttttccgt aattgctgct ctaggggcta
    22621 atattctagt tgctatcagc aaatttgtcg gctatgcaat cagtggcagt gccgccatgc
    22681 taaacgaaag catccacagt atcgttgatt gcagcaatca gattttgctg ttattcggtg
    22741 ataaacaagc caataaagga cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat
    22801 actttttcag tacaatcgtc gcaatgatgc ttttcttcgg cggtggggca cttggggtga
    22861 tggaagcttt tgaaaagttg acgcatccat cccatgaagt gggtaatccg atcattgtta
    22921 tagcaatact gttatttggt ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag
    22981 aaatcaaaga attgaatact gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac
    23041 acagtgaaat actgattatt tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg
    23101 ctttagcagg aacaatattg acttatgcaa ccgggattgc tgcatttgat gcgatcagcg
    23161 gattgctgat cggtttcttg ctgatgggag ccgccatttt cctagcaaaa gaattttata
    23221 gtctgatcat cggtgaaagc gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg
    23281 aacgtcctga gatcagtcgg ctgatcgata taaaaacagt tcatttaagt ccaacggaga
    23341 ttttagttgc agcaaaagca gatatcgttg gtaacaaaga gaatgaagct tactcggtca
    23401 tcaatgatat tgaaaaaaat atgcgtcagt ctcttcctga caagaagatg tatatataca
    23461 ttgaaacaga taaatatgac ccaaattatt ctcgtaaata aaacgtttat tcatgaattg
    23521 aagcaaaaat gagaaaaata taatcttgtt tttaaataaa
//
LOCUS       DADXTG010000030        23431 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.030, whole genome shotgun sequence.
ACCESSION   DADXTG010000030 DADXTG010000000
VERSION     DADXTG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.030"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(266..466)
                     /locus_tag="IWI57_001044"
     CDS             complement(266..466)
                     /locus_tag="IWI57_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2449203.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="IWI57_001045"
     CDS             complement(771..2003)
                     /locus_tag="IWI57_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBC2449204.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2257..2826
                     /locus_tag="IWI57_001046"
     CDS             2257..2826
                     /locus_tag="IWI57_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2449205.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3004..3444)
                     /locus_tag="IWI57_001047"
     CDS             complement(3004..3444)
                     /locus_tag="IWI57_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBC2449206.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3602..4366
                     /gene="map"
                     /locus_tag="IWI57_001048"
     CDS             3602..4366
                     /gene="map"
                     /locus_tag="IWI57_001048"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBC2449207.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4398..5321
                     /locus_tag="IWI57_001049"
     CDS             4398..5321
                     /locus_tag="IWI57_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBC2449208.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5397..6536
                     /locus_tag="IWI57_001050"
     CDS             5397..6536
                     /locus_tag="IWI57_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBC2449209.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6529..7329
                     /locus_tag="IWI57_001051"
     CDS             6529..7329
                     /locus_tag="IWI57_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2449210.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7329..8156
                     /locus_tag="IWI57_001052"
     CDS             7329..8156
                     /locus_tag="IWI57_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2449211.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8134..8868
                     /locus_tag="IWI57_001053"
     CDS             8134..8868
                     /locus_tag="IWI57_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2449212.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8968..9834
                     /gene="rfbA"
                     /locus_tag="IWI57_001054"
     CDS             8968..9834
                     /gene="rfbA"
                     /locus_tag="IWI57_001054"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBC2449213.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9848..10420
                     /gene="rfbC"
                     /locus_tag="IWI57_001055"
     CDS             9848..10420
                     /gene="rfbC"
                     /locus_tag="IWI57_001055"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBC2449214.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10442..11470
                     /gene="rfbB"
                     /locus_tag="IWI57_001056"
     CDS             10442..11470
                     /gene="rfbB"
                     /locus_tag="IWI57_001056"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBC2449215.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11568..12419
                     /gene="rfbD"
                     /locus_tag="IWI57_001057"
     CDS             11568..12419
                     /gene="rfbD"
                     /locus_tag="IWI57_001057"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBC2449216.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12454..14487
                     /locus_tag="IWI57_001058"
     CDS             12454..14487
                     /locus_tag="IWI57_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449217.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14531..15811
                     /locus_tag="IWI57_001059"
     CDS             14531..15811
                     /locus_tag="IWI57_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBC2449218.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16021..16827
                     /locus_tag="IWI57_001060"
     CDS             16021..16827
                     /locus_tag="IWI57_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449219.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16839..18059
                     /locus_tag="IWI57_001061"
     CDS             16839..18059
                     /locus_tag="IWI57_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449220.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18049..19635
                     /locus_tag="IWI57_001062"
     CDS             18049..19635
                     /locus_tag="IWI57_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBC2449221.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19674..21812
                     /locus_tag="IWI57_001063"
     CDS             19674..21812
                     /locus_tag="IWI57_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2449222.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22180..23163)
                     /locus_tag="IWI57_001064"
     CDS             complement(22180..23163)
                     /locus_tag="IWI57_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBC2449223.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7378 a         4007 c         4810 g         7236 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagatt tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt
     2221 ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact
     2281 agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt
     2341 actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt
     2401 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
     2461 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
     2521 cattttgcaa gaagagagaa gaagttgtat cgttcactgt gcttagaaga atatggtgga
     2581 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
     2641 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
     2701 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
     2761 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
     2821 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
     2881 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
     2941 tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt
     3001 atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc
     3061 ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa
     3121 agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc
     3181 gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac
     3241 gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc
     3301 ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag
     3361 attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc
     3421 atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat
     3481 gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca
     3541 tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa
     3601 tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt
     3661 agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat
     3721 tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt
     3781 tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc
     3841 tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa
     3901 aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga
     3961 ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg
     4021 gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc
     4081 gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt
     4141 tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat
     4201 cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc
     4261 gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga
     4321 tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat
     4381 aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc
     4441 gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg
     4501 tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt
     4561 taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg
     4621 agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag
     4681 gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt
     4741 tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc
     4801 gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg
     4861 ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga
     4921 actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca
     4981 tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc
     5041 cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg
     5101 ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca
     5161 tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg
     5221 ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt
     5281 tcaataaatt gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt
     5341 aaatttatat cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc
     5401 ttgtttcttt tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa
     5461 cacctgtatt caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa
     5521 gaataaataa agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta
     5581 tatcgacttt attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga
     5641 ttgcttcttt agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta
     5701 aacaaaaaag tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac
     5761 gtattgattc cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc
     5821 ccgtaacgat tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg
     5881 atgggctggc ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt
     5941 ttttcctcca tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta
     6001 ttatcggttt ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag
     6061 ctctattcct agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt
     6121 ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg
     6181 ctatcattag gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc
     6241 atcaccgact aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga
     6301 tggctttgat gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga
     6361 tcatattgat tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga
     6421 tcaatgagaa acatcagttt attttaaaaa cattgcgatt tttagggaat aaagagtacc
     6481 gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa
     6541 ttaaaccagc cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca
     6601 ctagataatt ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac
     6661 aacaattcac ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc
     6721 gatatctatt ttcatgatga aaattccgga tttggttatg gacataatca caatctgata
     6781 gctagcgatt ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca
     6841 gttacagcat tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag
     6901 attcttaatg aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat
     6961 tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct
     7021 gtgtttgaat gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt
     7081 agttttatgg tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc
     7141 ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta
     7201 ttgtacacac ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca
     7261 aaactgtttc gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt
     7321 tttttctgat gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc
     7381 aatctccagc ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta
     7441 tttacgataa tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt
     7501 atcatcatga tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag
     7561 cagatgaagg aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt
     7621 attttgaact attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga
     7681 ttttttctgg gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag
     7741 ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg
     7801 ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag
     7861 attttttgga tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc
     7921 ttcctgtacg aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc
     7981 gataccgttc tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt
     8041 tatccagtca ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa
     8101 accggcatat ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc
     8161 gtgcgttcgg tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat
     8221 agtattttat ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta
     8281 gatgatacat gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt
     8341 tcggggcctc gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga
     8401 gatctaatct ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc
     8461 actgactatt ttgagcagaa tccggatcag ctagtagtag tttctgattt aatcattgtg
     8521 gacagcgagc tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga
     8581 tggcttaata atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct
     8641 ttaaaacccg atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt
     8701 ttgctgggag gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat
     8761 gatttaaacg cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt
     8821 gttcatctga ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt
     8881 aaatccttaa tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt
     8941 tgaaatcaaa aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga
     9001 acacgtctgt accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa
     9061 ccaatgattt attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt
     9121 atttcaacgc cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta
     9181 ggactgcaga tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc
     9241 attggagaag aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac
     9301 tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca
     9361 gtatttggct atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat
     9421 atgcaagctt tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact
     9481 ggcctttatt tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa
     9541 cgtggagaac tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc
     9601 gtagaactta tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt
     9661 gaagcatcta cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg
     9721 gaagaaattg cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag
     9781 ccattgaaaa aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc
     9841 cttacagatg aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt
     9901 ctttggcgat catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca
     9961 tggattgaaa tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct
    10021 tcgggggctt catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac
    10081 tggagcagtt ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg
    10141 ggacggattt atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc
    10201 ccatggattc gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta
    10261 taatgcagaa gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc
    10321 aattgatcca tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga
    10381 atttgaagca gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct
    10441 tatgaaaaac atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta
    10501 cgtggtaaac aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg
    10561 aaacaaagag aacttggccg gacttcctag cgatcgcgta gaattggtcg taggagatgt
    10621 tgcagatgca gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc
    10681 ggcagaatcc cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat
    10741 cattggtacg tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt
    10801 atctactgac gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga
    10861 aggcgaagga gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc
    10921 tacaaaagct ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc
    10981 aaccatttca aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc
    11041 tcgccaagta acgagtatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa
    11101 tgttcgtgac tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa
    11161 aggcagaatt ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt
    11221 gatggaattg atcttagaaa tgatgggcca accaaaagac gcatacgacc atgtaaacga
    11281 ccgcgcaggg cacgatttgc gttatgcaat cgattcgaca aaattgcgag aagagctagg
    11341 ttggcagcct gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga
    11401 agaacatgaa gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa
    11461 tggacaataa tacagaggaa atttcactgt attaatggaa aaacacatgt gaggcaggcg
    11521 ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga taaccatatg tttttattaa
    11581 ctggaggaaa tggacaatta ggtacagaat tgcgccattt attagatgaa aaaaatctgc
    11641 catatgtttc aacagatgca aaagacttgg atatcacaga tgctgagaaa acaatggctt
    11701 atattacaga attaaaaccc gatgttattt tccattgcgc agcttacaca gcagtagata
    11761 aagcagaaga agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc acaagaaatg
    11821 tagcacttgc agcaaaagct gtgaatgcac aattcgtcta tatcagtaca gactatgttt
    11881 tcgatggaaa aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg
    11941 aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt
    12001 atatcatacg aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc
    12061 aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa
    12121 cttggacacg tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg
    12181 gtatttatca tttgtcgaac gaaaatagct gttcttggta tgagttcgca aaagaaatat
    12241 tgaaagatac agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa
    12301 cacgtccgca gtattcagtc atgagcctga aaaagacaga agcgttaggt tttgtcatac
    12361 caacttggca agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat
    12421 ttccttttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag aagtgcacga
    12481 cgaaaagaaa agccaaaagc gaatcaggca gattcgaatt tggtgcttga cctgtttcta
    12541 aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt taatttaaac
    12601 ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact
    12661 gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt gcagacacct
    12721 ttttacctct cacaagctga agaggacttt tcgttggtga acgaggatta ttcattaaac
    12781 ggacaaaaca tgatcattgc gtataattct cctgtaaagg atattacggt tatcggaaaa
    12841 ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt ttattgggcg
    12901 atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt aacgaagagg
    12961 aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc agtccagtgg
    13021 tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat gattgctttt
    13081 tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat gcttttggtg
    13141 gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt gatgctggcg
    13201 tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa agtttcatgg
    13261 gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg agtcatggtt
    13321 catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt atatccagga
    13381 aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt ccttacaaat
    13441 tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc cctgttctat
    13501 cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa aaaagatagc
    13561 gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat cttttggatt
    13621 accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt tcctccatta
    13681 agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt tatcgcttat
    13741 atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc agtaaatgca
    13801 tggtcttatt tctatgcgat tacgcattcg aaaatggata attatttttc ggattttgaa
    13861 accatcgcag ttgctgtgtt gggaacagtg attttagctt ttgcattatt tggctggaga
    13921 cgcttgtttt acctcgcttt tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca
    13981 gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc
    14041 gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa cttcacccca
    14101 acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc tgcatggaag
    14161 aaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca tacaagagct
    14221 tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg
    14281 ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa acgaccatta
    14341 actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc ggataaagac
    14401 ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca aacacaaact
    14461 caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg aaatattagt
    14521 agaaaggaga gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt
    14581 cggtttcttg cttttgttgc tgacgctagt cttttttgtt tggacagcag gggtaggttt
    14641 tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta gtgtggctat
    14701 tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc taggtgttgc
    14761 gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt ctttatttgg
    14821 agacttactg tttcctttga ttattttatt ttgtttagta attaaatgga tcgagtggac
    14881 aaaatgggat cgcggtattt atctgattgt ctttacggtc ttcctcggaa tcacgttata
    14941 tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc caggcaattc
    15001 gctttctgat atttggatca atacgaatac aatcggaagt tctttgatga ctcttgcttt
    15061 attgatcagt ggatttgcca gttcttttga acgatggtat gttcgaatca tcggtgggat
    15121 agcagtcgtt gcagcctttt taggtatttg ggtctgccaa tctagaggtg cattagtcgc
    15181 gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc
    15241 gccatttatt gcctacacgg ttacgattct tttagctttg ccgatttctt atctggcggc
    15301 tgtctctgaa aaagtcaatt tgtttaccga tcgagaagat atttggcata aattctatca
    15361 gacgctagga gaaaaatctg aacaagtttt actaggaatg aaaacattta tcttccagcg
    15421 aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata tctatggatt
    15481 gataggattt gggattgctg ctttgctgct tatccttttt attggtcgct taacgttgaa
    15541 agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg tgatgatgca
    15601 gtcatttatg gaggatacat tgacttcata cccatgggtt ccgattattt atatcctgtt
    15661 ggctatggcc gttcatcgat atgatgaacc agaagttgcg gaggaattct atgttatgga
    15721 agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac cagatagcgc
    15781 tcattcttcc aggatccaac gctatcattg ataggcacaa aatacgagag aggctgtgac
    15841 aaccgtcgca gccttttttg gtttattgca attgtctagc atcttctgta aaatagaaac
    15901 gttagagaaa atcttgtatc atagaaaatt aagaaagaaa taagaacgca gctcggtacc
    15961 aaaaatatgg taaaatggca cttgactgta attcactgaa aatcgcttgg aggaacacaa
    16021 atgtttaaag atttgtattt atttatgaag gacatttatc agaacagaaa attattgcta
    16081 cagttttcga tcaatgactt caaatcaaga tatgcaggat cgtttttagg aatcttatgg
    16141 gcgtttatca atccgctatt tactgtattg atttattggc tggtatttgg tgttggactg
    16201 aaagcacaaa tgatcgatgc aaaataccca tttatcgtat atttagttac cgggatgatt
    16261 ccctggttct tcttctcaga tgcatttgca tcaactacgg ttgtttttcg ggaatatact
    16321 tatttagtaa agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa aatcctatcc
    16381 aatctgtaca cccatttatt cttcattctg attggcatag ctgtcgcgct tataaatggt
    16441 atatatcctt cagcaatgtc tttacagctg atttattatt tattttgcat gtgtgctttc
    16501 ttgacaggat tgacttggct gacggcatcg atccaaccat ttttacctga tatcatgcaa
    16561 ttaattacga tcgtgctgca gttgatcatg tggacattac cgatattatg gagccctaat
    16621 cagtttgatt caggtcttgt aagcatattg aaattaaatc cgctgtacta tgttgttcaa
    16681 ggttatcgtg agtcattttt aagtgaagct tggttctggg agcatggaac atatactttg
    16741 tatttctggg ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg
    16801 aaaccacatt tttcagatgt gctgtaagga gaatagctat gacagaatat gcaattagac
    16861 taaaagatat cacaaaatct tataatatgt acgcaaaacc ttcggatcga tttcgtgaag
    16921 ccctcaatcc ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg
    16981 tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta
    17041 caatgttgaa aatcattacg ggagtactga caccaacatc cggcacgatg gaaatcgaag
    17101 gaaaaatctc agctttgcta gaactaggat caggattcaa tcctgagtat tctggctatg
    17161 aaaatatcta tttaaatgga atggttctag gattttcaag agaagaagtc gatgaaatgg
    17221 tcgatgatat cattgaattt gctgacatcg gagaccatat ttatcaacct gtaaaaactt
    17281 attcaagcgg gatgttcgtt cgtctagctt ttgctgtagc aatcaatgtg aatcccgata
    17341 ttttgattgt ggatgaagca ttggcagtag gcgatcttga atttcaacta aaatgtatgg
    17401 aaaaatttac agaaatcaag aactcgggaa agacgatttt attcgtttct catgatatca
    17461 gttcgattcg tcgtttttgt gatcgttcct attggttgaa aaacggagaa gtcgtcgaat
    17521 atggcgatac gatggaagta acgacgaatt atgaaaactt tttaaagaaa aaatcagtta
    17581 aaacagtgga tcggaataaa aaccaagtag aacggttcgc cgctccagat atcgtcgacg
    17641 tgacgaaagc ggaattgttg gataaagaca aaaatccggt ggatatgctg gaacaaggag
    17701 aaaaactata cgttaaagta acttatgaag taaaagatga tacaatcaaa aatccggttt
    17761 taggaattgc gctacgaaca gtcgacaatt tttatgtatg cggacttaat actctactag
    17821 atggtatcaa gattccttgg aaaaaaggag agaatatctt ttatctggag tatgaaaagt
    17881 taggtttgtt agctggtgaa tattattttg acgttgcggt ctttgaagaa aatgcaacgg
    17941 ttcctttggt atataagaca aagtacatga acttgtttgt cagcggttct tatatcggag
    18001 aaggcatcgt ggtcttagac cataaatggg aagaagggac tcatagcaat gaaatatgat
    18061 tttgaaatgg acctagatga acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca
    18121 ggaagtaaag ttttagagtt tggtcccgga aacggccgat taacgaaaca tttaatcgga
    18181 gcaaaacaat gtgaagtgag cattgttgag ttagataagg aattgttcga ttttgtcagc
    18241 gaatttgctc aagatggatt ttatggtgat atcgaaagtt ttgagtgggc aaactattat
    18301 gctggacaga catttgatta cgtcttgttt gcggatgttt tagaacattt ggtagatcct
    18361 ggaaaaacat tgaagaaagt cagagaattt ttgaatgaag aaggagagat cctgattaca
    18421 ttccctaact tggcgcataa ttctgtcatg atcgatttgt tcaataacca attgccgtgg
    18481 gcttcttatg gtctcctaga tgaaacccat aattcttttt atactcatga tggcttccaa
    18541 aaagtttttg aaaaagcggg attattcatt aatattgaag attatttgta cttagctgta
    18601 ggtgatacag aattaaaatc aacttatgaa gaattgccgg aagctgtacg ctatgatttc
    18661 aaaatgcgac catttggtga agtctaccaa tactttttct cattgatgaa acatccagta
    18721 gcacaaagtt ccattgcaga gccacaaaat tctaattatg taaaagtgtt agaagtaacc
    18781 cagcaaacga aacaagatga aacaatccaa caaataccat tcaataattt cacaggggaa
    18841 aatgagacac taagtttccc cgtatcagaa acaacagaaa gaatggtgtt tcgcttcgcg
    18901 caacaaccta gctttatcga attcagcgca gaagctgccg gagaaaaact aacatttatc
    18961 gattcgaatg ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg gaaagaatta
    19021 ccagagttcg ttctaacgga tatttctggg aaagaagtga ctatccattg tcactatcgc
    19081 tttatcgggg aattgacacc aacaatgaaa gaattgttgg aaacgattcg tcccatggct
    19141 gaagtcacaa agcagctttc tgagaaaaat gacgaattag aaagagaaaa tcatcatctt
    19201 cttgaggaaa atacaagact agaccatacg ttaaaaacaa ccacaaatcg ctattgcaca
    19261 ttgttggaaa gtgatgaatg gacaataaaa catagactag gacgacgtaa gaaagaaact
    19321 tcgaaaaaaa tccaagaaaa agaactttct atatgtattg acgagaaaat ctgggatgca
    19381 gaaacaaaaa tattaaaaat catcggatgg ggaatcgcta attcggatcg ccagcccttg
    19441 tcctataagt tatcagctga ccaatcgcca tttttcgaag caattcctgt ttcacgagaa
    19501 gaagtcaatc aagcagaaca gctcgcaaaa ggaacagaag cgggatttga gttacgaatc
    19561 ctttgcgagc aagaacgttc attcttagtg gaagcagtag ctcaaaatgg ccaatcatgg
    19621 atcattgaaa tgtaaataat gattaactta taaagataag gagtaaacga atcgtgcaaa
    19681 aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga acgggccact ggcgatttgc
    19741 ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat ccctacgata aaggtagaaa
    19801 aagaaaatgt gattgctgaa tcaacacatg tcgtaagatt ggatatcaat catttatata
    19861 atcttgatgt taagacccaa agcggtttca aggtacgttt atcagggaaa atgagaggaa
    19921 aagccatctt agacttccaa acagcgagac atcaaaatgg catagctgtg aaattaaacg
    19981 gtaaataccc ttatgatgac ggcattgaat cttcatggga aagaaaaaaa agattactga
    20041 aaaaagggat aaattatgca aggactcatg gcgtaaaaaa agcgatccgt cgggtcaaat
    20101 tggaattgaa gccaggatcg atcgactatg cagagtggat cagtcgtcat gagaaaccag
    20161 atttgaaaga gcaaaggaaa gaagcacaag gatttgcgta ccgtccattg atttccatac
    20221 ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat tcggtactgg
    20281 atcaaacgta tgatcattgg gaactatgta tcagtgatga tgcttctact gatccagcta
    20341 tccgaaagtg tctggaatcc tatcaagcaa aagatgatag aatcaaagtc gttttccgaa
    20401 aagaaaatgg acacatcagt ctggcaacta attctgcatt ggaaatagca gaaggagaat
    20461 ttatagcact cttagataat gatgatgaat taccgccatt tgcattatat gaagtggcaa
    20521 aagtgctaaa cgtacatcca gaactggatg tgatctacag tgacgaagat aaaatcgatg
    20581 cagatggcaa tcgctttgat ccgcatttca aagcagactg gtcgcctgat acattgatgg
    20641 gaaataatta tatctctcac ttaggcgtat atcgaacaag cattgttaaa gaacttggcg
    20701 gtttccgtaa aggctatgaa ggttctcaag attatgattt ggtccttcgt gtgacagaac
    20761 aaatccctgc agatcatatt taccacattg accgagtctt gtatcattgg cggacgatcc
    20821 caggatcaac tgcaagtaat ggcgaagcca aaagttatat ctatgattcc ggagtgaaag
    20881 cattgacaga tgccctgtct agaagaaaca tcaaaggaag cgtacgtcct ggcagaattt
    20941 ctggatttta tgagattgct tatgacgtct tgcaagagga tttggtcagt gtgatcattc
    21001 caacgaagaa tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt
    21061 cttattcaaa ttatgaaatc attattgcgg ataacggaag tacagatcca aaaatgcaag
    21121 aattatttgc agaatacaag catcagctga aagaccgctt tatcgtagaa ttgattgaca
    21181 ttccattcaa ttattctcga attaacaatt tagcagcaga aaaagcaaac ggcaaatact
    21241 ttttattctt gaataacgat acagaagtga ttgagccaga ttggatgacg gtgatggttt
    21301 cttatgcaca gtttgatcga atcggctgtg taggagcaaa actgttttat ccagatgata
    21361 caacgcagca tgcaggagta ttattaggaa tcggtggtgt tgcagggcat gcattgaata
    21421 actatgatcg aacacattgt ggttatttcg gccgattggt cattgacgtc aattacttag
    21481 cagtcactgc agcttgtatg atggtcaagg cagctgactt caatgctgtt aacgggtttg
    21541 atgaaacatt agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg
    21601 gtcgttacaa tgtctatgca catcaagcag aattgtacca tttcgaatct aaatcaagag
    21661 gttatgaaga tactccagaa aaacaaagac gttttgctgg tgaaatcaaa aaaatgcaag
    21721 ataaatggcc agcatatatt gcgcatgatc cattttacaa tgataattta acaaaagacg
    21781 gaatcggcga cttttcgttg agaccagatt aaactataat aaaaagctgt tatgttgtga
    21841 ttcgctcgca cataacggct ttttatagtt ttttgtcaaa aaaaggcaga aggtgccgac
    21901 tcaaaaaaat aagtcgaggt tgggacagaa gtgtttaact ccgagaaata agaagaaatt
    21961 cacgaaaatt gcttttcaaa tttttgtgaa tttcggctta tttaagaaga agttgcttct
    22021 gctcccaccg tttattcgtt tttaaagaat gagacaaaag tgtttcttac ttttgtccca
    22081 cgctcttatt catgctcaga gcgccaatag atacaaaatc atagccaaag tttcacgaac
    22141 cctagctgtc aaagaatcga tattttattt taatataggc tattttaaaa taaatggttt
    22201 aaggtattcc tctacaaaag ccactctatc aattatcatg ggtttaaaat aagaagtgat
    22261 ggagataacc agattgttct tagcgtcaat ataaagaaca tttccactat taccaattgc
    22321 tgaatatatt tcgttctcag aatctaatac ccaccataaa tagccatatc tcatattttg
    22381 taagtcttct aaagcactgg ttctaggttt gaacatctcg tttatccaca tgggtggtac
    22441 gatttgtttt cctttataca tccctttatt taggcataat acaccaattt ttgccagatc
    22501 acttgccgat aaacacaaac catatcctgc cgtcccgata tttagtggat cacaaaacca
    22561 gacattggtt ttaggagact tatccattgt aaactgctta tgttcttcag ctgtttcagc
    22621 ataataattg acatggtttg gtatccctat aggatcaaat agatacttat tggcaaaatc
    22681 aacagttgtt agcttacttg tctgatacaa caagccagtt aatatgtgta aacagacagt
    22741 ttgatagttg aattcgccgg ttataccttt tcttcctccc aataaatcta gtgatgtttt
    22801 tgtccaatct tcacttgaac agacttttgt ccatggatcg cctttacatt tgtagggtgc
    22861 tttcattgtt agcaagtgct tgattgtaat agcgtaaata gttttctccc cgcgtttcac
    22921 tttatactct ggaaaataat ctaatacttt atcatcgata cttttgatta atccttgctc
    22981 taaagcaatg ccaatcagca aagatacaat acttttcgtt gctgacatga tatgacaagt
    23041 atcgtctttt ttataaccat tccattcatc tgtataaact tctaccccat ccttatatgc
    23101 gacaatttga catatatttg gttgtcgaaa accaataaaa tcatgcaact cttgtttatt
    23161 cataagatat tacctcactt tcttctataa gcataatgtc attaaaaatc gatttcaatt
    23221 ttttcgattc ttcacgtaat acaggtattc ttatggacta gacagcgctt tgggaagatg
    23281 gattctctga aaaatataaa ggttcacttt atgagtggat aaaaaaagca gcaaatactt
    23341 cgacttcttt aatttaccaa cattagtgaa aatcgattca tctggagatt tgtaatttag
    23401 agtccttgtc ttgattagag gtgtataaag c
//
LOCUS       DADXTG010000031        23094 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.031, whole genome shotgun sequence.
ACCESSION   DADXTG010000031 DADXTG010000000
VERSION     DADXTG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.031"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..2560
                     /locus_tag="IWI57_001065"
     CDS             119..2560
                     /locus_tag="IWI57_001065"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBC2449224.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2890..3594)
                     /locus_tag="IWI57_001066"
     CDS             complement(2890..3594)
                     /locus_tag="IWI57_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449225.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3781..4026)
                     /locus_tag="IWI57_001067"
     CDS             complement(3781..4026)
                     /locus_tag="IWI57_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449226.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4043..4894)
                     /locus_tag="IWI57_001068"
     CDS             complement(4043..4894)
                     /locus_tag="IWI57_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBC2449227.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5060..6064
                     /locus_tag="IWI57_001069"
     CDS             5060..6064
                     /locus_tag="IWI57_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBC2449228.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6305..6661)
                     /locus_tag="IWI57_001070"
     CDS             complement(6305..6661)
                     /locus_tag="IWI57_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBC2449229.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7029..8216
                     /gene="tuf"
                     /locus_tag="IWI57_001071"
     CDS             7029..8216
                     /gene="tuf"
                     /locus_tag="IWI57_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBC2449230.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8404..10995)
                     /gene="mprF"
                     /locus_tag="IWI57_001072"
     CDS             complement(8404..10995)
                     /gene="mprF"
                     /locus_tag="IWI57_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBC2449231.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11021..11782)
                     /locus_tag="IWI57_001073"
     CDS             complement(11021..11782)
                     /locus_tag="IWI57_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2449232.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12060..12581
                     /locus_tag="IWI57_001074"
     CDS             12060..12581
                     /locus_tag="IWI57_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBC2449233.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12640..13500
                     /locus_tag="IWI57_001075"
     CDS             12640..13500
                     /locus_tag="IWI57_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBC2449234.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13685..16000
                     /locus_tag="IWI57_001076"
     CDS             13685..16000
                     /locus_tag="IWI57_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBC2449235.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16182..18023
                     /locus_tag="IWI57_001077"
     CDS             16182..18023
                     /locus_tag="IWI57_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBC2449236.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18047..19156
                     /gene="rpoD"
                     /locus_tag="IWI57_001078"
     CDS             18047..19156
                     /gene="rpoD"
                     /locus_tag="IWI57_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBC2449237.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19376..21475
                     /locus_tag="IWI57_001079"
     CDS             19376..21475
                     /locus_tag="IWI57_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBC2449238.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21671..22534
                     /locus_tag="IWI57_001080"
     CDS             21671..22534
                     /locus_tag="IWI57_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBC2449239.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22639..>23094
                     /locus_tag="IWI57_001081"
     CDS             22639..>23094
                     /locus_tag="IWI57_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBC2449240.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         7783 a         4037 c         4812 g         6462 t
ORIGIN      
        1 atataaaaaa gagaatattt aaggattgtt aaaatttaat cttagaaaaa aataactgga
       61 acaagagcta tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt
      121 gaaagaagtc gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt
      181 aagtccattt actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac
      241 aaaactgaaa aaagataaaa tcgatcaagt aatatttgga aatgtcctgc aagctggtaa
      301 tggtcaaaat gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc
      361 aatgacgatc aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct
      421 gatccaatta ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc
      481 acctatgctg aaacagtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat
      541 ggtcaacgac ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa
      601 tgttgcaaca caattttctg tgagcagaga agagcaggat cgctatgcct tgtcgtccca
      661 gttgaaagca gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa
      721 gatttctgat gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga
      781 aaaactgggc acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc
      841 ttccccgttg aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa
      901 taataatctg ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag
      961 tatcatggga atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa
     1021 tttatcaaca atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt
     1081 tagccaagaa ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt
     1141 aggccatcca attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct
     1201 tcgtgaacag aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc
     1261 agtcttgtta gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta
     1321 tcagcttacg ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga
     1381 aactgcgttg atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga
     1441 aaaccaagtg agtgaagtgg aaatcccaat ggggatcgcg cagaattttc aaatcaatgg
     1501 caagaaaaaa tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa
     1561 tggtgcaaaa atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca
     1621 aatcgttcta tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa
     1681 agaagagctg attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg
     1741 tgttcaagat atctctactc gggaattcat ggggagtttc catgcatact tatctattga
     1801 ttttttagtg gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag
     1861 tgtggcaaac aagttacggg agtggttccc tgaagaagag attctattca gtattttaag
     1921 taattttgct acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg
     1981 gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc
     2041 gaagcttgac ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc
     2101 tgtagtggca gctacaggaa atgatacacg tgcggttagc gcttcgatcc acgcctacgc
     2161 ggcaagaaat ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt
     2221 tggaaaacta acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc
     2281 aaaagcaaaa gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt
     2341 aattgctgca gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg
     2401 aatccaaaaa ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat
     2461 tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca
     2521 gacagctata caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag
     2581 aataaagcaa gatttacagt tattttattt actgcagatc ttgctttttt ctctatcaga
     2641 aaatatgttt attattagaa ttttttatag ttaaggttag gcatcttaag aaatcaggca
     2701 actccaagat atcttcaggt agaagagaag tcgcctaaca ttgtaggatc ataggtgatt
     2761 tgttggacag gtcaagctga tatttgagcc tgatgttaga aggaatttct ttttacttgc
     2821 tgtagaaatt attatatgac taaaaaggct gggattttaa atcccagcct tttatgacaa
     2881 atagaatttt tatcgatcag taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa
     2941 agtcaatttg actttgtatt tccgttcgag tgctttttcg ataacgaact cattttcagt
     3001 agtcatagaa atagttgcta atttacgagg ttcatcagca tttttttcat taataactac
     3061 ttcatgttca ttttgttctg tgtaaaattt aggatactca gcaacggctt ttgaaaaatc
     3121 agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt
     3181 tggtttttcc gttttttctt ctggcacact gactgtttct tctgtaactg gtgtcgcaac
     3241 agcttgcgca gttggttcat cgtttttcaa tcgttgatca ttccagtaag acacgatttc
     3301 aataagaaga ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac gaattctctt
     3361 taatacagta ttgaagttgt catcgattgg cacgttgatc cgtgtatctt catttgtttt
     3421 cccagctaat ccgatcgtta agatttgacg gtttttggga tcaagcatga cttgtaacgg
     3481 caaactataa cgtcgcaagc tctcttcttc tagagaacgg aataattgtc ctcttacacc
     3541 atccaatgaa ggacggaatt gatttaataa tttttgtttc tgttttttct tcatatgttc
     3601 tctccttcgc tcataccctt tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat
     3661 gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt tcccgttttt
     3721 tgaaagaaaa ataaaaaatg gaacggatta tgtatatata cataatccgt tccattcttt
     3781 ctattttttc ggtatcaagg caaaaacagg tagttcaggt gaataaatga cttcatgttc
     3841 gtgccagggt ttgaccatta gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg
     3901 atcatgtgtc cagtttcttg caggaaaatg ttggattttc tctgcatcga tcaaaaagac
     3961 aaaatcggaa tgaaaagtct gggtaacttc ttccgagaga gaattcaagc gttcgattgg
     4021 atccataaaa tcacctttcg acttaaaatt gggtagtatc tgggtctttt gctttgccga
     4081 ctacaccatc taactgatcg atttgtttca tttcttcttc tgttaattca aaatcaaata
     4141 attcagtatt ttctttgatt ctgcttggtg tcactgactt aggaagagga aggaattcgt
     4201 gttgcagcga ccaacgcaaa gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt
     4261 gtttcatttc aggcacatcg aagattttac cagtgcctaa tggactatat gcttcgagga
     4321 tcatatcatg tttttttgca tactcaacta cagttgtctg taattcgcct ggtgcaagga
     4381 aaacttgatt gaccatcggc ataatttttg ctgtttttgc tagtgtatct agatggtggg
     4441 gtaagaaatt gctcacaccg attgctttga ttttacctgc ttcatacagt tcttcaaatg
     4501 ctcgccatgt atctgcattt gcttgttccc aattatcacg aaaagcaaca gggtttggcc
     4561 agtggatcaa aaacagatct aaataatctg tttggagctt cttcaatgat tcttcaaaag
     4621 aagacatcac aagttcatag ctgtggtttg cattccataa ttttgttgtc aagaatatct
     4681 cttctctagg aattccactt tccttaatcg cttggccgac gctttcttca ttcttgtatc
     4741 cttgggcagt atcgatatgg cgataacctg cagccagcgc ttctttaaca gaggaaacag
     4801 cgacatcccc atcaggtgtt tgccatgtgc cgaatcctac taccgggatt tcgtaaccat
     4861 ttgatagacg ataagtgctt gttaatgaat tcatactttc agctcctttt ttattcgttc
     4921 aactataagt gtagcgcatt tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat
     4981 ttttgtttaa aagaaaatga ttttgattct ggtatttttg attttttttc cgtaaactaa
     5041 gacagaaggg aatgagaaaa tggatatcta tttaaaaaaa gcaattctgc atattattga
     5101 cagagaaact ggttctccag tcttttcaca aaaagagctg gatctgacaa aagaatatat
     5161 tcgtgatttt cttcaaaaga agatccaaaa aatctcttct gctcaaacaa aaactggaca
     5221 actagcagaa gaaagcatga tcgttgaatc acttaaaatc gctcaagaaa attttgtgga
     5281 agcaagtgaa gcaatcgttc agcggtggtt cgatatctac caagaaagtg aagaagcacc
     5341 tagtgcagat gtatttgttg tcttgtatga attagataca gtgatgtact tatctttatt
     5401 gaaagtcaat tatcgtgagg cttacactca ctttgttgaa gcagacgaag cagggatcga
     5461 caaccgcttg attttgaatc gagcaattct aggggcgaaa acacaaaaag cagatgaagc
     5521 attcgctgtg aatttgactg attggaccta cgaattaatt gaaaaaaaat atgagttttc
     5581 tggcaaaaaa gagctttatt tttctggaag agtgatcgaa agtgttcctg caccttcttt
     5641 agaagaaaat gtaaaagtga tccaaaaagt agccaagcat cttggaaaaa aatttgagac
     5701 agaagaattt gatatcatgg ctgatttgaa agaagctgtc tatgatacga tagaagaaaa
     5761 aggtcggttg gatcatgaga tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa
     5821 aatggctttt aaagaagaag tacaagagca aggatttgtc ccagaagcac ctcctgtaaa
     5881 agaagtacaa gaaatttctg agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg
     5941 gatcgaactg atcgtaccag tagatgtata tcgcaaccca gatttgattg aatttgtgaa
     6001 taatccggat ggtacgatat ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt
     6061 ataaaaatga ctagaatatt aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc
     6121 aagagaagga tcggaaaaga cgaatataac aaaaaaagag cgtgagacaa aagtaaagaa
     6181 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttctta
     6241 tttcttggag ttaaacgctt ctgtcccagc ctctttttat ctaataaaat tttaagacca
     6301 tttactaatt atcgattttt tctgccgcat ttttaggcac ttcagaagcc tttatttctt
     6361 cccagcttaa tacaccttta cgaggattga ctttgagttt aagatacgca ttcatcttta
     6421 atggatgttc tctttcttcg cgaagcgtca cctgtttttt ttcaccatct ttattataag
     6481 ccgcttgctt gtaggtataa gtcgtgaatt tctctccgtt atctgcttgg gtagctgaca
     6541 tttcgccatt cgtcgtgatt tttgtataat aagcttcgcc gccgtaaaaa taatgataag
     6601 ctgcaaaact tccgccgaga aatatgccta aaacgacaag tacgccaatt aattttttca
     6661 tgtacttcac ctctttcctt attattaacg aaatagaaga tttcgtctat tgaaagttgt
     6721 tggcaacagc tgaattttaa acttttcctc attttgttct tattgcttca aaagaaagtt
     6781 aaggactatc ttgaagtttt tctcaaaaaa cacatttttt tatttataaa aggtactttt
     6841 ttcgcgtata agacagacga gtgctcttag ttgactttga ccaatattga cgtataataa
     6901 gcttagttcg aatcttacaa aaagtaagtc ggacgtattc tttttgattt agtcaaaaaa
     6961 agactataat aaaaaggtga gagctgaaat ttttcagtga tctatattat ttaggaggtt
     7021 tgtaaattat ggcaaaagaa cattatgaca gaagtaaacc acacgttaac attggtacga
     7081 ttggacacgt tgaccatggg aaaactactt taactgctgc gatcactaca gtattaggca
     7141 aaaaaggcct agcaaatcct caagactatg ctagtattga tgcagctcca gaagaacgtg
     7201 aacgtgggat tacgatcaat accgcacacg tagaatatga aacagaaaaa cgccactacg
     7261 cgcatattga tgcaccagga cacgcggact atgtgaaaaa catgattaca ggggcagcac
     7321 aaatggatgg tgcaatctta gttgtttctg caactgacgg tccgatgcct caaacacgtg
     7381 aacacatttt attgtcacgc caagttggtg taaaatacct gattgttttc ttgaacaaag
     7441 ttgatttagt cgatgatgaa gaattgatcg atttggtaga aatggaagtt cgcgagttat
     7501 tgagcgaata tggtttccca ggcgatgaca ctcctgtgat caaaggttcc gcattaaaag
     7561 cattgcaagg cgatccagat gctgaagctg ctattatgga attgatggat acagtagatg
     7621 aatatatccc aacaccagaa cgtgatacag ataaaccatt actattgcca gtggaagacg
     7681 tcttctcaat tacaggtcga ggaactgttg cctcaggtcg tattgatcgt ggtgctgttc
     7741 gtgtcggtga tgaggtagag atcgtaggga tcaaacctga aacacaaaaa gcagttgtaa
     7801 caggtgtaga aatgttccgt aaaacgttag attacgggga agctggggat aacgtaggcg
     7861 tgttgttacg ggggatccaa cgtgacgata tcgaacgtgg acaagtactt gctaaaccag
     7921 gttccattac tccacataca aaattcaaag cagaagtgta cgtgttgaca aaagaagaag
     7981 gtggacgtca tactccattc ttcaacaact atcgtccaca gttctacttc cgcacaactg
     8041 atgttacagg aacaatcaca ttgccagaag atacagaaat ggtcatgcct ggtgacaacg
     8101 tgacgattga tgttgacttg atccatccaa tcgctgttga aaacggaacg acattctcaa
     8161 ttcgtgaagg cggacggaca gtaggttcag gtatcgttac agagatcgaa gcataagaaa
     8221 cgcgactcga atattttggt aatgaaaaat gtttaaaata aaagagcggt ccaaacagga
     8281 tttctgtttg gaccgctctt tgttcttcaa ttgtataaga agagattgta gaagcatgaa
     8341 ctttcgtttt acttcagtct cttttattca tcatgagagt gttcatctat cggtatgcag
     8401 tttttaatac tttcttcgta tccatttttt caaaccactt attctacctt gtgtctgctc
     8461 aactggcgca ttatcaataa tcagcaaagc aatcataaca tatgcaatcc aactatctct
     8521 agaatataga gtataacgtg agacccagtt tgtagcgtac ttgcttttat attctcttaa
     8581 tccatggaaa gaataaaaat gagaaccaaa ctcgtaaacc aagtatgcaa tcctttcctg
     8641 taaaaaactc tttctggaag ttccgacatt tgaaagaggg gccatcccta aattgaacca
     8701 ttggatatgt tcttctttca tataggcaaa caaatgcaag aacaagaaat ccatactgcc
     8761 agaaggtgct tttttagaat catatcgcat cagatcaatc gttcctactt tattgttcgt
     8821 gtaagtaggc atgatcgttg caaaagcgac aatttcttct tgggtatttt tgacaactgc
     8881 aataggcgct ctttgtagat aatcttcaga gaaaaagcct aaagaaaacc ctttttcctt
     8941 tcgtgaaccc aaccaattat cagagatatt tttgaaagta ctcatttgct cagtagaaaa
     9001 tggtggttgt aagacatcaa atgtaaatcc ttctcgttcg atccgattca agacagcacg
     9061 agtgcccttc atttttttcc ctgaagttgt aaaactattc aggtctacat aagcttcttc
     9121 ccccatctta atgaaatcgt agccgaattc atgcaaaatc ataacaatct cttcgcttgt
     9181 ttcgtagaaa actggtaaat agcatagtcg atctgtttct tcgataaaag cttcgattgc
     9241 ttctggaaaa tcttctttct taccagaagg atcacccatt acaatacatt tattattgaa
     9301 gcaagcgaat tggagaagaa cagttggttc accgtctttt tcataggcaa acatccgctt
     9361 atcttttaag aagatcaatt gactatcgga gtttcctcca taagttgtaa ggatcttcag
     9421 ggccttttct tcattaaagg cttcgccaat ctgtttcttt tctccttgaa ggaaatgaac
     9481 aaatagaacg atcatgaaac taacagcgat gatggccaac aatcccgaaa accaaatctt
     9541 ttcagaagga aataagaaaa aggaaataaa atggtgacgc cgatgaggga aatccggcag
     9601 attgtatacc ccaataacga tgtaaagcaa ggacagtgca ccaataatga ttccatcgat
     9661 cgtacgccac tcccatgaat agatcaattg ttcccgaaat aattcgtttt tagaagcaag
     9721 aatgatcaga agcagaatag acaagaaaac caccggtgtg aaactaaaat cacttaataa
     9781 aacataaagt aaagctagaa caatcaagaa gattgtaggc aaatatgctc gtttcacacg
     9841 tgctgcgatc cctctgccca taatgattaa aagaaatccc aatagtatac taggaaattg
     9901 gataataaaa tgaaatttca atgggttcaa actatgaagc cagcgaaact ctgtaaaagc
     9961 ttgcgggata gtggcagaaa gtactagcat gattccagaa aagtataata agacaaccac
    10021 gattttatgc gcaatttctg ttgctaattg tttagggata ccagaataac gctgatcaaa
    10081 agtggaaccg atatttttga agaaaaagac gattccaatc aaaaacggaa tgatgtagta
    10141 gaacaggcga tagagcaaga tccacacaac gactatttct cttggcacac caatagcgga
    10201 caacccgata atcatcatta catcgaaact cccaatttct cctggaatca tagacacgat
    10261 tcctatcact gaagcagcaa tgaataatgg aattgtttgc cacagatcaa tccggatttc
    10321 cattaaataa ccaacagaaa ggaaactaaa caggacaccg gtccattcca acaatgaaac
    10381 tagcaatagc tgtccttgag ttttaagtga ggtaccacca aggaatcctt tctttttgaa
    10441 agtagtaaat aaaaagacga caggaaaata tagaccgccg ccgatcaacc atatccaata
    10501 ttgcttcaaa aaaggagcaa ctggcgtgaa gactacaagt aaaaaagaaa gcagactgta
    10561 aatagaaagt ccagccatca aaaacaagaa aactttcgat aatgcttgga tcacgtttct
    10621 gctctctttc ttttgtccat atagttcaga gcgaagcccc acactaacaa acccgccgaa
    10681 accggcaata ttgttgatcg tattgatcag ccagcttgtc tcaaacaggt aacgaggatt
    10741 ctgcttttgt tcaagcatcc gattcaaaat aatatcataa ccaaccatag gtaacacaga
    10801 gataagacca atcaatagca ttagtccgct tttccataca ggtatagtgg caaacgtttc
    10861 tcctaattgc tggatagata gagttttacc gatggacatc aattcaccga caataatgac
    10921 aatgacagaa atcaaaaaga ttgttttgaa cagtcctagg tggtttttca gccattgtat
    10981 ggtatttttt aacaaataaa tcaccttcta ttcctaatag ttatgtttca gaaagaaatt
    11041 gaagaacttc tcgattgaag cgttcaggat cttttctagc taattcatgt ccttgttctt
    11101 tcacaagcac aaaggaagct tttggtatag ttttagctat atacaaagaa tgttttagct
    11161 tgattacatc tttttttccc acgatgatca aagtggggga gttgattttt tttaaatcat
    11221 tttctgtaag cccgatgtca tgcagcaaaa gtttgataac taaaagattt ttgcgaagac
    11281 ttgggcgaaa taaagagagt aaccataccc atgcataatg aatattactg atgactctgg
    11341 cagaaaaacg aacgccttta acaagcgtgt ttccagaatt taaaatcaat cgatgaactt
    11401 ttttagggaa gctgctggca aataccagtg ctagatttgc tccatcacta aatccaagaa
    11461 aatctgcctg atcgattttt tccagcaaca tgatcgtatt cagatcctcg gccattaact
    11521 gaaaattcaa catagatgct tcgtttgtcg aacgaccatg tcctcggcta tctactagat
    11581 acacggtata gtagcgttcg agtacaggga cctgttctga aaaaaagcgg ccgctgccgt
    11641 cattgccatg cagtaaaaag agagggaagc cttgaccgct cttttcatag taaatttcgc
    11701 tcccatctgg catttttgca tatttctttt cttttttcac ataatcacct actgtgtaaa
    11761 gtataacgat tatatcagtc attgggcaat tgtttttact gaaaaacaac gatcattttc
    11821 gtaagatgtc aaaaaataca ggaaaattca ttttttctta aggcgtacac agaaatcaag
    11881 gaagaaatag ccaagaagat aaagaaatag taagataaaa aaagatgtca aaataactag
    11941 gtgatgaaat gaagcgaaaa cgattatcta ttatccgtat tcttttttac atgatcattc
    12001 tgacggttat tggtgcggtg ttgtttttat gaggaagaac ttatcgttcg gtaaaacgag
    12061 tggaaagcta tcaaatagaa ataacaacag ctgcaaaaaa ttatcatatg gaaaactacg
    12121 agaatttgat caaggcgata atattgaccg aatcaaaagg aaaagggaca gatctcatgc
    12181 agagctcgga aagtctgcat ggagaagctg agatcattga taatccagct gacagtattg
    12241 atcaaggtgt caaatatttg gcggaaatga tagtagaagc aaagaaacaa ggatgcgatt
    12301 tatggacagc tgtccaagca tataattttg ggaaagatta tattgcctat gtaaaaaatc
    12361 atggcggaga aaacacagta gaattagcag aagcgtattc taaagatgtg ctgagcccac
    12421 ttttaggaaa tgaggaaggg aaacaatacc gatattggcg gatccagtcc ttgctttaca
    12481 atggcggcta tctctatcat aatggaggta acatgtttta tcctgatatt gtcaaaatga
    12541 atgaaaaaat aatagagacc tatgaaaaag tctttaagta aaatagcagg aaatgttcaa
    12601 tcatgaataa aattaaatga aagaaggagt gcgcaaatag tggaaaaaag gcaaaaaatg
    12661 gaaaatcaag aagtggggta cacaaccaaa gactggatgt tacggtttgt gaagggaatg
    12721 tttatcggtt caggatttat cttgccagga gtcagcggtg gtgcattagc agctgtcttt
    12781 ggcatgtatg agcggctgat ttctttttta gcccacctta caaaaaattt caaagaaaat
    12841 gttttgtttt ttctgcctgt tggtctaggc ggagtgacag gcatctttct actttctttt
    12901 ttagtcagtt ttttattagg tgcgtatgaa acaacgatat tatggttttt tgtcggatgt
    12961 atcgctggaa ctgttcctgc gttatggaaa gaagcaggga aaaaaggaag agatacttct
    13021 gatgtgatca tcatggttgt tacttttgtt ctggcttatt tgtttttatt atacggcgct
    13081 caattatttg acgggcaagt cgatcagaat tttggaacat ggctgattgc cggcggattg
    13141 attggtttgg gtatgatcgt cccaggattg agcccttcta attttttggt gtatatggga
    13201 ttgtataaag cgatggctga cggatttaaa gaagtacgac ttgaagtgat tgtgccgatc
    13261 ggaattggtg ggattctttg tgttcttgga ttgtcaaaaa tcatggatta cattttctca
    13321 aaggcatata gcaagctatt ccatttcatt ttaggtgtag tttttgcttc aacgattatg
    13381 atcattccta ccgattacgc gggattggga gttgctgggg tactagcttg tcctgttctt
    13441 tttatagccg gtacagcact aggttggtgg atgagccgtt tggaggaaca atataagtaa
    13501 agatagattg ataataaaaa cagcacacgg gtgattcaaa caaaaaggtt ggcatctttt
    13561 ttgtaaaatc attcgtgtgt ttttctataa attttataaa aacaagttgt ttttaatctt
    13621 ggaacacgct agacttaaac acgtgattat ttttgaaagt tggcggaaaa tggagaaaaa
    13681 gaaaatgaat ggacttacac agcaagaggt agttcagaaa atggcttctg gccagcaaaa
    13741 tgactaccag gaagatgcat caaaaagtac aaaacaaata ttcagcgata atatattgac
    13801 gttattcaat tttctgaact ttggtattgg gatttgtctg ttatttgtag gtgcgtattc
    13861 caacatggcg tatttagcaa ttattgtcgt caatatcgtt attggaattt atcaagaaat
    13921 acatgcaaga aatcttgtac ggaaattatc gattgtttcc caatcagaag tactagtgat
    13981 ccgggacggt aaagaacaat cgatttctac taaagagctg gtattaggag atttggtaaa
    14041 aatcggagca ggagaacaga taccagccga tttacaggtt atatctggac gggcagaagc
    14101 aaatgaagct cttttgacag gcgaatctga tttgattgtc aaagaaactg gagctgaatt
    14161 gctttctgga agctttttga caagcggttc tgtcatggct gaagttatcc gagtaggtgc
    14221 agataattat gctgtccgct tgactagcga ggcaaagact cacaaagcta ttcgttctga
    14281 acttgtagat tcgattcgca aagtatccaa attcactagt ttcgtgatta ttcctttggg
    14341 gatcatacta tttttggaag cactgttcat ccgcgatgct tctatcaaaa tttctgttat
    14401 tgcatctgca gcagctttgt taggcatgct tccaaagggt ttggtattgc taataagtat
    14461 tgcattaacg acaggggtaa ccaaactggc aaaaaaacga atccttgttc aagacatgta
    14521 ttcaattgaa acgctggccc atgtcgatac attatgcttg gataaaacag ggaccattac
    14581 tgaagggaaa atgagtatcc agcgggtaga agcactacat ccgctgtacg agaaagccat
    14641 cccagaaatt atggggacgt atctggcaga aagcagtgat aacaacatca ctatgcgggc
    14701 tatgagagat tattttgagc tttctaatcg gtatgatgtg acaaacaaag tcgcattttc
    14761 ttctgatcgc aaatggggcg cgatggaatt tccagaactg ggagtcgtgt acgtaggagc
    14821 gcctgaacgt ttggtggaag aagatcggct tccggacagt ctaaaaacag cccaagcgaa
    14881 tggctatcgc gtacttatgt tggggatcgc tccaaatcag caattgaacg aagaaaaacc
    14941 agcatttgtt gagccggttg ctctgtttga aatcgatgat ccaatccgaa aaaacgctca
    15001 agaaacacta gcttatcttc atgaggaagg tgtggatctg aaagtgattt ctggagacaa
    15061 tccttttact gtctccaata tcgctagacg tgcaggatta ccgaattatg atgcgtatgt
    15121 cgatctttct caaattactg aagaaatgga agtgcgagaa gctgctcatc gctatacggt
    15181 ttttggacga gtgagtccgc agcaaaagaa attgctggta aatgaattga aagaaagcgg
    15241 cagaacggtt gcaatgacag gcgacggtgt gaatgatgtt ttggcgttaa gagaagcaga
    15301 ttgcagcatt gctatggcag aaggtgatgg agcaacacgt cagattgcga atctggtatt
    15361 gttggactct gattttacta cattgccaga agtcctgttt gaaggacgcc gagtggtcaa
    15421 caatgtgacg aaagtctcag gcatcttttt tatcaaaacg atttattcat ttattttatc
    15481 gattatatgt gccgttacag caattggatt tccgtttatc cctatccaaa tcacgctgat
    15541 tgatttagcg attgaagggt atccttcctt ctttttatct tttgaacaag ataaaagaaa
    15601 aatcacttac cgttatttgc ctacagcact tattaatgct ttaccaaatg ccttgcttgt
    15661 agtagtgaat atcattgcgg tgtatctact tggaaaaaca ttcgcatggg gaaatgctga
    15721 aactactact ttgatgtatt acttgctgat tgggatcagt tgcatggcag ttgtaaaagc
    15781 ttgttatccc ttcaacccat tgcgcatctt cctagcagta acgacgatcg tagggatata
    15841 tgtagctgca atgctgttcc atggaatttt ggaagtaaac ttagggttgg gagagagcgt
    15901 aggatacttc tttgtattca tgctcgtaaa tattgttctt cgagtattgt actggaaggc
    15961 tgatattcaa aatcagttac tgaaaagagt agcaaattga tgcttaatga gatagaaatg
    16021 tgttatactt gaaaaagcaa tgaaatatga tacaatcaaa catgtataat tgtgtccttt
    16081 aaaatcgcac tgatttcagt tagtgcgatt ttttaccggt tattctcctg aaatatcttc
    16141 tgttaaaccc aaaagatatc aatagagcag ggggtgaaaa aatggctcaa cggattcctc
    16201 aagaggttat cgaaacagta cgcagtcaaa cgaatatcgt tgaggtgatt ggacaatatg
    16261 tccagttgaa aaagtctggt aaaaattact tggggctgtg tccttttcat gaagaacgta
    16321 caccgtcttt ttctgttgca gaagacaagc agatttttca ttgctttggt tgtggtaaag
    16381 gcggaaacgt gtttactttt cttcaagaac tagaaggtct aagttttcca gaagcagtgg
    16441 taaaagtggc agacttcgaa cagataccgt tagaagatca atggcgtcag cgtactatag
    16501 ctgtccaaaa tcctgaatct gctacgggga aactgattgc tgctcatgaa aaagcagcgg
    16561 aaatctatca tcacatgcta ttgcatacaa aagctggaca gccagcttta gaatatttgc
    16621 aaagtcgtgg gttgtcttta gaattaattg aagaattcaa cataggtttt gcacctaatg
    16681 agcgttcttt tcttcagcaa gtattcaaga atgaatcaat ttcaaatgaa ttgttagaac
    16741 gctctggatt gtttgtcacg catgatgatg gccgtatgtc tgatcgtttt tatcaaagag
    16801 tgatgtttcc acttcgtaat tctcaaggcc agacgatcgg tttttcagga cgctggcttg
    16861 cacctgaaaa agggcaggaa aaagatcagc ccaaatactt gaatagtcca gaaacagaac
    16921 tgttcaataa aagacaagtt ttatttaatc ttgataaagc aagaagcgat atccggaaaa
    16981 acggagaaat tttgttgttc gaagggttta tggatgtgat tgctgcatgg caggctggta
    17041 tcaaaactgg attagcttct atggggacaa gtctaacagc tcaacagatc caacaaatgg
    17101 aaaagctgac gaaagaattg gtcttttgct atgacggaga caacgcaggt tttgaagcaa
    17161 ccaatcgagg gattgaattg cttcggcaaa acagtcgatt agcactttct gtcgtagtcg
    17221 ttccagaaaa gcttgatcct gatgaatatc tccgtaagta tggagaagca tctttccaag
    17281 aattagtggc tcatgggcgc gaaacagtct ttacttttaa atcacgttat ttaaaacaag
    17341 ggaaaaattt agataatgaa aaagacaaga tcgaatacct cgaagaatta ctagttgaac
    17401 ttagccgagt tgtttcacca atcgaacaag atatgtatct atctcaactg tcaacagagt
    17461 ttcagatcgg cagagagacg atacaaaaac aactgcgcga tcttcgaagg acaaatcagc
    17521 aaaatcgtca agaacctcag gaaagtcaac acactcaggt tacgagacaa gaaactgcac
    17581 ggaaaaagag acctttgacg caagtagaaa aggcagagca gattctatta tatcgtgcat
    17641 ttaaagaagt ttctgtccgt aatattttaa aagaacgaga atttcagttt attcatgatg
    17701 catatgcaga agtttatttt ttattcgatg catatgtctc acaacattat gaattcaacc
    17761 ttgcagagtt tttagacttt ttgcaagatg aaaatttaag gcggttggtg gtggaaatcg
    17821 aatatttacc ggtggcagaa gaaagttcgc cacgagaaat ccaagacttg ctagatgtga
    17881 tcagcaaatc tggcttagct gatgaaataa cattaaaaaa acaaatgcag caagaagcca
    17941 gacgaactgg aaacaaacag cttgagcttg aattgacgat tgaaatcatc aatttaacga
    18001 aacagttaaa acaagccaaa tagctactca aggggggcct tcttttatgg caaatggaac
    18061 agataaaaaa acgtatgaag cagcagtagc agctttcatc aaagaaaaca aacccaaacg
    18121 acaagttgta tatgatgatt tatccaatca gcttgctaca ccattcactt tgaatgcaga
    18181 tgaaatggac aaattgatcc aaaagattga agacgcaggt atcagtgtgg tggatgaaaa
    18241 tggtgatcca tcaattcaca gcttgaagag tgctgagaaa aaagctgaac aagcaaaaac
    18301 agaagatctt tctgcgccaa ccggtgtaaa aatcaatgac ccggttcgta tgtatctgaa
    18361 agaaattggc cgtgtctccc ttttaactgc ggaagaagaa gtagcgttag cattgaaaat
    18421 cgaagaaggc gaccaagaag cgaaacagcg tctggcagaa gcaaaccttc gtttagtagt
    18481 aagtatcgct aaacgttatg taggacgagg catgcagttc cttgacttga tccaagaagg
    18541 aaacatggga ctgatgaagg cagttgaaaa atttgactac cgcaaaggat ttaagttttc
    18601 aacctatgct acatggtgga ttcgtcaagc catcactcga gcaatcgctg accaagcacg
    18661 tacgatccgt attccagttc atatggttga aacaattaat aaattgatac gtatccaacg
    18721 tcaattgctg caagaccttg gaagagaacc tacgccagaa gaaatcggtg cggaaatgga
    18781 tcttccaaca gaaaaagtcc gagaaatctt gaaaatcgcg caagaacctg tttcccttga
    18841 aacgccaatc ggtgaagaag atgactctca cttaggtgat ttcatagaag accaggaagc
    18901 aacaagtcca gcagaacacg cagcttatga gttactaaaa gaacaattgg aagatgtact
    18961 agatacattg actgatcggg aagagaatgt ccttcgctta cgttttggtt tagatgatgg
    19021 ccggacgaga acattggaag aagtagggaa agtatttggt gttactcggg aacgtattcg
    19081 tcagatcgag gctaaagcat taagaaaatt gcgccatcct tctcgttcaa aacaattaaa
    19141 agatttctta gaataagcgt ggactattga ccagaagaga aatcagtaaa actgtttggt
    19201 ttattttaaa agaaaaaaag cttgagacta cgcagtctca agcttttttt tctataggaa
    19261 ggaaattcat tcattatttt taagtaaatc ctaaaaataa gctattggcg tgacgtatag
    19321 aatatgatag attaaatggt agcgtaaacc tagtaaagga gccgatcgct aagtgatgaa
    19381 aaaatgtcca aattgcggat cgaaaaaaat ggagcagaat actatctgtc cagactgcgg
    19441 tcatcaaata actgcagaag atcagcaaat aaaagaatca attgaagaaa caaatacaga
    19501 tgcagctatg caggataaat caactgaaga tgcagtcttt cctgatacag aattaaacga
    19561 tccaatcgaa tggtcggaat tgaaagattt acctctagaa tctgtaatgg aattattcga
    19621 aaaagcagaa ccagaagacg aagaaccacc tgctcacgat caagtaacta atatagagaa
    19681 aaatgaacag aagaaagcaa ctgttcatga gacttcggaa gaatcaggaa agaatcaaga
    19741 agaaacgctt gaatcaaaac aaaaaagaag agtagctgaa ctgaaagaaa tcgtcgataa
    19801 tgaggaagaa aattcgatac tatccgctta tatcaaagca catcgtgaag atacaaaaga
    19861 agagcacgcg aaagagctgc tcaaaatgat cagtgaaaaa attgcacagg aaaacaaagt
    19921 agagccagaa aaggaacaag aaactgaaaa agactctgat agagaaaacg ccacaggtaa
    19981 agaaacagta aaaataaaag acgtaaaaac ggatatcaca gaaaaaactg aagtatttac
    20041 tgaaaaacaa ttagaaaagg aaaaaacgaa taaactgccg gaagcagaga aaaaagaaat
    20101 actcgaaaac acagacaaaa agtcaacaga gcccgatgaa ataaatagtg aatccgatgc
    20161 tgaaaatcaa tctgtaagca aagagggtac tgatcttgaa gcaaaaatag aaaaagaaga
    20221 gaaccagcct aagctgtttg aagcagaagc gaaatcaata acaatcgtgc cagatactga
    20281 aaaaacacat caagaatcaa acttagaaaa aacagatcca gataaagcgc aaaagaaaaa
    20341 atcaaaaaaa gtaccttatc tcgttttagc agcagtactt ctactcggtg gaggaagctg
    20401 ggcttattat gaccatcatc aaaaagtaga agccgaagta gcagcagaag caaaaagaca
    20461 gcaacaaatg gaagaacttc atgagaaact tgctgccttt tatacagatg atcaccatca
    20521 atttattcgt gcagatatga ttcatcaaga tttatcaaaa ctaaaaaatg atttatccag
    20581 tcttaagaat gaaaaaggat attcagagtt agaaacaact taccaagata tccaatctaa
    20641 gatccgagat atccaaaaag tgaatgagtg gttcatagca cctgccatcg tcgatgatca
    20701 tctagcagat gacatcaagt tgaaagcaga taaagagatc caagcaatcg aaacggataa
    20761 cacagatttt ggaaaattga ttcaacaagc acaaacagaa gctaaaaatc aatatgctga
    20821 attgcaaaca gctaaagaaa aaacagctat agtctttaaa gacggaaaag tagtggattc
    20881 agcaacacgc gaccaataca agacggcaaa agaagcagta gaaaaagtga aaaatacaga
    20941 tctatctgct cagttgaatg aacaattgaa acaagtagat aagatgctca ctgataaaga
    21001 gaaaaaagaa gaagaggaaa agaaaaaagc agaagaagcg aaaaaagcag ctgaagaaca
    21061 agcaaaacaa gccgctcaag cagcagctgc agctcaagcc gctcaacaag ctgcggtcgc
    21121 acaagcagca cagcaagaag cggcatcctc gaccccttcc tcttcaaact cagcgaatca
    21181 gccaatcaaa tctgccagag caagtgatat tgccgatgca tctaatccag catggacgtg
    21241 ggcaccaggt gtccaaagca aagtgattgc tacatgtatc caacgtggat atatcgttga
    21301 aggaggttac agattagaaa aagccagaat cgaaaatggg gaaggctatt ataatctata
    21361 tgccacttca acaaaatctg cattgatgaa aggcattgga gaaagtgctt tgccatttta
    21421 catcgtaacc atcaactgta aaactggatg gttcggagga aatgggagca attaatttta
    21481 ggcaaatgaa agaggctgtg agcctgccgt ttaacaccgt ttattcgaaa aaggggagta
    21541 tgacacaact gttgtcatac tccttttttc acctttccaa accaaggctc agctattcaa
    21601 gtagataaca ttatgttatg atagcaaaga aatgtattaa gaaaaccttt cactatagag
    21661 ggagaaaaaa atgaacgatt tattagcaac tatatttaca ggtattatta ttgatgaaaa
    21721 tgaaaaaaac tactttgtcc aaaaaaacgg acaaaccttt ggcttaagca aatcagaagg
    21781 tgaacacaaa atcggtgaag cagttgaagg attcggttac caaaatcaaa aacatgaaaa
    21841 tcggatcact acacaaattc ctaaaagccg tatcggtcat tacgcttttg gaatcgttac
    21901 tgctacacga aaagatttag gtgcgtttgt cgatatcggt cttccagata aagactttgt
    21961 cgtatctctt gatgaattgc cgacaatgac agagctatgg cctaaaaaag gggatcgctt
    22021 gatgatttcc cttagagttg atgcaaaaga ccgtatttgg ggcagtttag cagatgaaaa
    22081 aatattcaaa tctttaagca aatatggaac agaagaaatg aaaaacaaag atgtgatagg
    22141 aacagcatac cgcttgaaat taacaggtac gtacgtttta acggaagatt tctatcttgg
    22201 gtttatccat ccttctgaac gatataatga acctcgtcta ggcgagcaga taaaaggtcg
    22261 agtgattggt gtccgaccag acggtgtact gaatctttca ttgaagccaa gagcatatga
    22321 agctatatca gatgatgcac aaatgatttt aaccatttta gaacgttctg cagatcagca
    22381 aatcaacttt acagataaat cggatccaga agaaatcatg aaagcttttg gtatcagcaa
    22441 aggagcattt aagcgcgcat taggtaatct tttgaaacaa ggattgatta cacaagaaaa
    22501 cggtgtgaca aaactagtca aaaaatctaa ttgagaatcc cttgcatgtt tttcttaact
    22561 agcttataat aattttaatt aacatagtgt tatatctttt tttatttgta attattataa
    22621 attgttgggg ttgagtaaat ggtcatgaat gctatttcgg cgatgaaaaa aacaaaacag
    22681 cagctacatg aagcggggtt taagctgaca ccgcaaagag aagctactct gcttgtgctt
    22741 ttggaaaatg agaaagagca tttatctgca gaagaaatct tctttttagt gaaaaaaaag
    22801 agtccggaaa ttggacttgc tactgtttat cgaacccttg aaatcttgac agaattgaaa
    22861 attgtcgata aagtaagttt taatgatgga ttagcccgtt atgatttgag aaaagaaggc
    22921 gccaaacatt tccatcatca tttactatgc ttgaactgcg gcacgatcga ggaagtagaa
    22981 gaggatcttt tacttgaagt ggagcaagta gtagaagaac gttatcattt tttagtaaaa
    23041 gatcatcggc taacgttcca tggaatctgt caggaatgct atgaaaaaga aaaa
//
LOCUS       DADXTG010000032        23063 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.032, whole genome shotgun sequence.
ACCESSION   DADXTG010000032 DADXTG010000000
VERSION     DADXTG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.032"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWI57_001082"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWI57_001082"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBC2449241.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWI57_001083"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWI57_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBC2449242.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWI57_001084"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWI57_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBC2449243.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="IWI57_001085"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="IWI57_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBC2449244.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWI57_001086"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWI57_001086"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBC2449245.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWI57_001087"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWI57_001087"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBC2449246.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWI57_001088"
     CDS             complement(8053..8523)
                     /locus_tag="IWI57_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBC2449247.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWI57_001089"
     CDS             complement(8569..8994)
                     /locus_tag="IWI57_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBC2449248.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWI57_001090"
     CDS             complement(9061..9330)
                     /locus_tag="IWI57_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBC2449249.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWI57_001091"
     CDS             complement(9341..10936)
                     /locus_tag="IWI57_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBC2449250.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWI57_001092"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWI57_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBC2449251.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWI57_001093"
     CDS             complement(14489..15049)
                     /locus_tag="IWI57_001093"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBC2449252.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWI57_001094"
     CDS             15402..16376
                     /locus_tag="IWI57_001094"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBC2449253.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWI57_001095"
     CDS             16546..17946
                     /locus_tag="IWI57_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBC2449254.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWI57_001096"
     CDS             complement(18024..18746)
                     /locus_tag="IWI57_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2449255.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWI57_001097"
     CDS             complement(18825..19331)
                     /locus_tag="IWI57_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBC2449256.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWI57_001098"
     CDS             19454..21253
                     /locus_tag="IWI57_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBC2449257.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IWI57_001099"
     CDS             complement(21321..22895)
                     /locus_tag="IWI57_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBC2449258.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6313 a         5008 c         4213 g         7529 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aat
//
LOCUS       DADXTG010000033        22971 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.033, whole genome shotgun sequence.
ACCESSION   DADXTG010000033 DADXTG010000000
VERSION     DADXTG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.033"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(187..1035)
                     /locus_tag="IWI57_001100"
     CDS             complement(187..1035)
                     /locus_tag="IWI57_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBC2449259.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1259..3199)
                     /gene="thrS"
                     /locus_tag="IWI57_001101"
     CDS             complement(1259..3199)
                     /gene="thrS"
                     /locus_tag="IWI57_001101"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBC2449260.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3536..3715)
                     /locus_tag="IWI57_001102"
     CDS             complement(3536..3715)
                     /locus_tag="IWI57_001102"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBC2449261.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3762..5585)
                     /gene="cadA"
                     /locus_tag="IWI57_001103"
     CDS             complement(3762..5585)
                     /gene="cadA"
                     /locus_tag="IWI57_001103"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBC2449262.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5863..8142
                     /locus_tag="IWI57_001104"
     CDS             5863..8142
                     /locus_tag="IWI57_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2449263.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRSYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8235..8882)
                     /locus_tag="IWI57_001105"
     CDS             complement(8235..8882)
                     /locus_tag="IWI57_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449264.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9061..9780)
                     /locus_tag="IWI57_001106"
     CDS             complement(9061..9780)
                     /locus_tag="IWI57_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2449265.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9911..11062)
                     /locus_tag="IWI57_001107"
     CDS             complement(9911..11062)
                     /locus_tag="IWI57_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBC2449266.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11279..12019)
                     /locus_tag="IWI57_001108"
     CDS             complement(11279..12019)
                     /locus_tag="IWI57_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2449267.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12023..12346)
                     /gene="rsfS"
                     /locus_tag="IWI57_001109"
     CDS             complement(12023..12346)
                     /gene="rsfS"
                     /locus_tag="IWI57_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBC2449268.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12403..12996)
                     /gene="yqeK"
                     /locus_tag="IWI57_001110"
     CDS             complement(12403..12996)
                     /gene="yqeK"
                     /locus_tag="IWI57_001110"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBC2449269.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12980..13630)
                     /locus_tag="IWI57_001111"
     CDS             complement(12980..13630)
                     /locus_tag="IWI57_001111"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBC2449270.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13640..13951)
                     /gene="yhbY"
                     /locus_tag="IWI57_001112"
     CDS             complement(13640..13951)
                     /gene="yhbY"
                     /locus_tag="IWI57_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBC2449271.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13967..15076)
                     /gene="yqeH"
                     /locus_tag="IWI57_001113"
     CDS             complement(13967..15076)
                     /gene="yqeH"
                     /locus_tag="IWI57_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBC2449272.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15073..15600)
                     /locus_tag="IWI57_001114"
     CDS             complement(15073..15600)
                     /locus_tag="IWI57_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBC2449273.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15780..16565)
                     /locus_tag="IWI57_001115"
     CDS             complement(15780..16565)
                     /locus_tag="IWI57_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBC2449274.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16558..17427)
                     /locus_tag="IWI57_001116"
     CDS             complement(16558..17427)
                     /locus_tag="IWI57_001116"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBC2449275.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17428..18807)
                     /locus_tag="IWI57_001117"
     CDS             complement(17428..18807)
                     /locus_tag="IWI57_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBC2449276.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18815..19234)
                     /gene="fabZ"
                     /locus_tag="IWI57_001118"
     CDS             complement(18815..19234)
                     /gene="fabZ"
                     /locus_tag="IWI57_001118"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBC2449277.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19259..19735)
                     /gene="accB"
                     /locus_tag="IWI57_001119"
     CDS             complement(19259..19735)
                     /gene="accB"
                     /locus_tag="IWI57_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBC2449278.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19738..20973)
                     /gene="fabF"
                     /locus_tag="IWI57_001120"
     CDS             complement(19738..20973)
                     /gene="fabF"
                     /locus_tag="IWI57_001120"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBC2449279.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20992..21729)
                     /gene="fabG"
                     /locus_tag="IWI57_001121"
     CDS             complement(20992..21729)
                     /gene="fabG"
                     /locus_tag="IWI57_001121"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBC2449280.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21741..22658)
                     /gene="fabD"
                     /locus_tag="IWI57_001122"
     CDS             complement(21741..22658)
                     /gene="fabD"
                     /locus_tag="IWI57_001122"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBC2449281.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22765..>22971)
                     /locus_tag="IWI57_001123"
     CDS             complement(22765..>22971)
                     /locus_tag="IWI57_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBC2449282.1"
                     /translation="QEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINEIEDEFD
                     VKIETEDGIKTVQDLVTYVEKQTA"
BASE COUNT         6305 a         5107 c         4005 g         7554 t
ORIGIN      
        1 aaaatcaact ttttgtttca cagcaatcac ttaaagttac atatccaact atttttttca
       61 ttaaaaaata aacttatttt tgaaaattat gttttcttaa gtcgtgaaac actttgatgg
      121 atcatttatt tgtggaaaac tacgttttgt tattctaaaa aacttatata aaaaatagta
      181 aggtttttat ttcgaatcca taaaatattc ttggattgct tctcgtgttt ttttcacttt
      241 ttcgctaagc ttttcgtttt tagccatata gaaataaaaa atgatatcga tcgtagcaaa
      301 ttgggccaaa agtgaattag ttgccgcact tcgattcgtg atttcttttg gtctagatgt
      361 ttgtacgagt acatcggcta tcttgctaag cgggttattt ccaaattgcg tcaacgcaat
      421 gatttcgata ttcatgcttt tcgccagctc ggctaatgcg actacatctg cacttctacc
      481 tgagtttgaa acaagccaaa gcactttctt tttctcattg ctgacaagtt gcgggagcaa
      541 gacgtggata tctttttcaa aaatgattgg cttaccgaca cgagaccatt tttgcagaat
      601 atcttctact actaaagaag aggcgccaac cccatataaa aaaacagtct ctgccgattc
      661 caaacactct acgacttttt ggatcgtttc ttcttctaga atatctactg tttcttttat
      721 cgctacttgt gcattgttac caagtttgtt tttgagagag taaaaggact catttgcttc
      781 tatatcacta aaaccttcat cgattccagt ctgtagttct gttgaaatcg tgattttgaa
      841 gtccgtcaga ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa
      901 cccggaagct tctgctaatt cattagctgt cattgtaggg accttctcaa tattaccgat
      961 aatgtagttg gccaactttt tttctgctga agaataatct tccatcatac tttcaatacg
     1021 actaattacg ctcataagtt cctccaccct tttaatgata gtctatgtaa gtataccata
     1081 tcagaccctt attcacggtc gtatactgga taaaaaacaa aaaaacaatc cgcccaacag
     1141 aatatcttat cccatcaatc ccacagattc tattgagcat ctgtgtcttt gaggaatacc
     1201 tgttatctat tgttcggatt gctttctatc tactactttt ttacaggaaa tttacctatt
     1261 agttttctct gctgtaattt ttgacttctg cttccatagc atcaataaag atattcaaat
     1321 cttctacagc tgtttcttta cttccataac gacgtacgtt cactgttgca tcttccaatt
     1381 ccttgtctcc aacaactaat tggtaaggga tcttttgtgt ttgagaggca cgtatcttat
     1441 agcccatttt ttcattacga tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt
     1501 ctttgatttc ataagcataa tcgccatgtg catcaacaga aacagggatg attgttgctt
     1561 gtataggtgc caaccatgtt ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa
     1621 aacgttccat tgtagaaaca attccgcggt ggataactac tggacgatgc gtattttccc
     1681 cgtcttctcc aacatatgtc aaatcaaagc gctctggcaa taagaagtct aattgtatag
     1741 ttgataatgt ttcttctgta ccaagagctg ttttgacttg aacatctagt tttggtccgt
     1801 aaaaggctgc ctcgccttct gcttcaaagt aatctagttc taattcatcc atagcggctt
     1861 tcagcattgc ttgggctttt tcccacattg cgtcatcatc aaaatattta tctgtattgt
     1921 ttggatcgcg atagctcaag cggaaacgat aatctgtaat attgaagtca gcatatactg
     1981 ccaccatcaa ttcaagcgta cgtttaaatt catctttgat ttgatcagga cgaacaaatg
     2041 tatgaccatc attcaacgtc atttctcgta cacgttgtaa acctgacaaa gcacctgatt
     2101 tttcatagcg atgcatcatt cctaattcag cgatacgaat tggtaattca cgataagaat
     2161 ggatcgtgtt tttgtagacc atcatgtgat gagggcagtt cattgggcga agaacaagca
     2221 tttcgccatc tcccatatcc attggcggga acatgtcttc ttgataatga tcccagtgtc
     2281 cagatgtttt gtacaactca acatctccca ttattggtgt atatacatgt tggtaaccta
     2341 gactaacttc tttgtctacg atataacgtt cgatcgtgcg tcggattgtt gccccttttg
     2401 gaagccagaa tggcaaacca gaacctactt ctggggaaac catgaacaag tcaagttctt
     2461 ttcctagttt acgatggtct ctttcttttg cttcttcacg gagacggata tattctttca
     2521 aagctttctt gtcaaaaaag gctgtcccgt atacacgttg catcatttga ttgtttgagt
     2581 ttccacgcca ataagcacct gccactgata ataatttaaa tacttgtata cggccagtgg
     2641 atggaacatg tggtccacga cacaaatcga tgaattcgcc ttgttgataa gcagtaatca
     2701 cttcttcttc tggcaattca ctaatcagtt ctactttgta cggatcactt gcaaaaattt
     2761 ctaatgcttc ctgttttgaa agaacacggc gttcgatcgg cagattttct ttgacgattt
     2821 tcatcatttc tgcttcgatt gccggcaaat cttctgcagt tacttggttt tgtccattat
     2881 ctgtatcgta gtaaaaccct gaatcgatag ctggtccaac gccaaagtgg atatttggat
     2941 agaggcgacg catggcttgg gccatcaaat gagctgctga atgacggact aagccaagtg
     3001 catcttcatg gtctggtgtg atgatctcaa tacttgcatc ttcctcgatt ggacgagtca
     3061 aatcaacgag ttttccgttc actttaccag ctaaagcttt ttttgctaag ctttttgaaa
     3121 tgctttcagc aattgccaat gttgttgttc cagactcaaa ttctttgact gcaccatctg
     3181 gaaaagtaat ttttatcata atagtttcct ccttgaaatg ttttgattga tcagatcatc
     3241 aagctgaaaa acaaaaaaat cctagcatct cttgaaaaga gatactagga cgaaaagttc
     3301 gtggttccac ctaatgttca aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg
     3361 cccttgcttc aacaagggtt tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt
     3421 cagcctagct tttcctctct tatcgaagac tctcaaattc agttggcttt ctcatcaatc
     3481 aattatgtgt ttatttaacc actaataaat agaaaaatca agtgggaaac taaacttatt
     3541 ttttccattc cccatttaca ccgtatgttc cttttttcat ctcattgatg atgacatgga
     3601 tgtgttcttt tggtgcctgc gtgtttttat gaacagcttc cgtgatatct ttcatcatat
     3661 tttctaattg ttccggactg cgaccttcta ctaattcgac atgtacgaat ggcataatgc
     3721 tctctcctct ttctgatcgt tcttttttca actcgattct atcatactat gtttgtcttg
     3781 ggtactgaat caggaaaaat ggctggatct ttttttgctc tcaaggcact caaaatagag
     3841 accgtatcca cgacttcttg caaaacagct ccgaataatg cggggattag tcccgtactt
     3901 gcaattaaca tcaaaaaaat acaaataaag atgccaatca gtacagactg tttcgctatt
     3961 ctcatcgtat ctcttgaaat cactactgct tttgctacac gcaagagatc atctttcaag
     4021 atgaccgcat ctgcactttc actagcagct gtagaaccat gagcccccat tgcaataccg
     4081 atatctgcag cagcaagtgc tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt
     4141 ctttgatctt tagggatact ttctaaaatg gtcagtttat cttgaggcaa gctacgggca
     4201 tgtacagaat ctaattggac tttttctgca atcgtttgag caatggcaga tccatccccg
     4261 gtgatcatca tcaaattttt cacatcaagt tttcttagct ccttgattgt ctttgctgct
     4321 tctgggcgaa tctgatccga aaataggatc gttcctgcaa aagatcgatc aattgttaca
     4381 tagattgctg tattggacgt ttctatttct tcagctgaaa caaaagaggc acgtccaatc
     4441 tttacttcat gattattgac tactgcttgg attccttgac cggtcacttc tttcagttga
     4501 ctaacaggat acagttggct gtttttcacg ttgttcacta acgaacgagc taagatatgg
     4561 ctagattcct gttctgcact agctgcatac agaagtagct cttctttttt tattccattg
     4621 actggctgaa tcgcctctac ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa
     4681 gcaatcgttt ttgtacgtgc gagcttttca atcgttgttc cagtttttac aacgatcccg
     4741 tttttacttg aacggctcat tcctgcaact aaagcaatgg gcgcagctag aatcagtgga
     4801 caaggtgatg ccactaccag tacctcagca aatcgtaaag ggtcttttga gaaccaccaa
     4861 gaaataccac cgatcaaata agcaatcaac gtgaaaggga ctgcataacg atcagctaaa
     4921 cggacaaatc gggcaggctt ttcttttgat tcttcgacta gtttaataat catttgatac
     4981 tggctgtctg cggcacgttt ttcagcgatc atttggatcg atccgtctcc atttaaagac
     5041 cctgacataa tctgatctcc aggtttcttg gtttccgggc gggactcacc tgtcaaagaa
     5101 gattcatcga ccgaagattc tcctttggtc actcgaccat ctgcgggtac aagttcgcca
     5161 ggcttgacta gtaactggtc atcaatattt atttcatcga ttactatatc ctgcaatttt
     5221 ccttctttga atcgatgggc aattcttggg gtgttatcta acaaagactg cagttcttta
     5281 cccgctcgtt tattcgcgta atcctctaga ctatctccgc ccgtaagcat gatcaatatc
     5341 atcaaactag cccagtattc tttgacggct aatgttgcaa ggattgcggt aatcgctaat
     5401 acatctacgc catatcttcc tgaacgtaac gtcttgatca tttccaaaaa catcaagaag
     5461 gcgagaatcc ctccagtaaa tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca
     5521 aattcactta ataaagctat cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt
     5581 gtcattgttt tttctcctct tttatgtgac ttagagaggt tagtaataaa tttccagctg
     5641 cctctttcat cttaagcata gtccttgact agcacttttt aaaacaatag gccttaattg
     5701 aaaacaacta tcactatcag cgtttccaaa attcatcagt tctacaaaca aaaatattca
     5761 gcaaggatta ctttttcgat tcatcagtta aaaagtacct tttttcgaaa aactatgcta
     5821 tacttttaat tcgttattta cggcggaagg agcaaacatg cgatgaatga gaaacaacta
     5881 gaacaaacgc acgtggatga aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat
     5941 acaaaacaac aagaattgac tcaggaaatg aatcattcga caaaagacac agctaatcac
     6001 aagattcgag ctggttccaa tgaagctttt tatgaatctg ctgttgaata tcgccaacat
     6061 gagcaggaat tgcttttgaa ataccatact cttgaagcac agcaaaaacg cctgcaaacg
     6121 ttgtctgtca tgaaagatag tccttatttt gcccgtatcg actttaaaga agaccgtgaa
     6181 acagaaacat tatatctagg tatcgcttct ttaagagatc tggcagaaga aacaatcgtt
     6241 attgactggc gcgcaccaat tgccaatctt tattatgaag gtgagattgg tcccgcattt
     6301 tatgaaactg aaacagatag aatagatgtc gatctgcagt tgaaacgaca atttaaaatc
     6361 caagaaggac gtattttatc tatggtagat acgtcggaag taatcaatga tgactttttg
     6421 ctagagatat tggatgaatc atcaagcaac catatgaaaa acatcgtctc tacgatacaa
     6481 aaagcacaaa atgcaatcat tcgggacaca acaagtaaag taatgctgat tgaaggaatc
     6541 gccggaagtg gaaagacatc cgctttgctt caacgtattg cttttctgtt gtatcataat
     6601 cgcaagtggc tcgaggctga aaacgttctt ctattttcac ccaaccattt gttttctgat
     6661 tacatctcga ctgtattgcc ttcattaggt gaaagcggcg tgcctacaca gacattcaaa
     6721 aattatttgg tgcagctgct gccagagttt tctttactag atgaacagca acaagaaagt
     6781 ggttttttgc taggggaaaa agatccgata caagtgatga aaagcggatt gaccttagtc
     6841 gatcagattg atcgctatat ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg
     6901 aaaataaatg gccgtacgat cctatcaaag gaatcgattc gccaatggta taaagaaaca
     6961 aatgaacaat tacctttaca tcatagactt tctctattac aaacgaagct cctgaaaaaa
     7021 ttaggcggtc tgcaaaaaga cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa
     7081 cagctccaag aactatacgc aacagatcca aatcttgaat atacagaaca aaaggagcgt
     7141 tcattacgta aaaaactggc caaccaaatc gtacgtaaaa gattccgtcg aatccatcga
     7201 gggatcatga attatcaatt tgtcaatata acgaaacaat atctccactt tctacaaact
     7261 gtgccacttt ctcttctaac acgacataaa atcagcgaga ccaggtggca ggaacatttg
     7321 acaactgttc gtacacactt gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc
     7381 tttttactga tgcgccgatc atatccagtg acggttacac acaaagctcg ctatatcttt
     7441 attgatgaaa tgcaggattt cgcaccatca caagtggcat tgctgcaagc cttgttccca
     7501 aaagcaaatt tgactttctg tggagatcta aatcaaaatg tctttggaaa tgaaacgatc
     7561 gttggatcat tggaagacct tttcgtggac cgtgaagtca ctcgtttcca attaacgaca
     7621 agctatcgct ccacaaaaga aatcactgat tttgctaatc attttattgt gaaagaacat
     7681 caagtagaaa caacagcaag aaaaggagac cttcctttgc tcatccaagg aacaacagat
     7741 gatatcaaat tgaattggtt agcagatacc attcgaacga ctcagggaaa agcccggtat
     7801 tggcgtacag caatcatagc taaaacacaa gaagaatgcg aagctcttta tgctcaactg
     7861 ccagacgagt tgaaacaaca agtccagctg attttaaatg agagtgactt tatgaagcgt
     7921 tccattattt tacttccagc ctttttagca aaaggtctgg agtttgaccg agttttcttg
     7981 tgggatgtag atgcagaaca tttttcttct cagcaagaca aattgatcct ttacaccatg
     8041 tgtacacgtg cgatgcatga attgaccctt tttacctcca ataatgaaag tcctttgtta
     8101 aacaatatgg atatttcatc ttatgagaaa aaagtactgt aagtaaaaag acaactgcta
     8161 tcattaatgt tagcagttgt ctttttattt acaataataa ttgcagaaaa agtaattcaa
     8221 tcatttaagg tcatttattc tatatataaa tccaaatctg tttcaggata ttttaatttg
     8281 acaagcgaaa taatcatttt cagttcttcc atgttttctg gatattctgg gtcaaatacc
     8341 ttgctatgac ggccaatccc gccattgtcg atcaatcggt aaattcgatt ccattcaatg
     8401 aattggtcaa ttgattggaa attgctgatt ggatagccgc cacaatccaa aatctctgat
     8461 acacgaaatg ctaggtatgc actatcgatc actttatttt ttttcgtgtc tatcttctca
     8521 tttctttcga taagaatttt ttcaggacga tgtttcaagc tccactctaa tagtagaaag
     8581 aaagcctcca tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa
     8641 agcagcggaa tcagataatt ttgtctataa atcagcattg gtgcgcggtc caccatacac
     8701 tctaaataac ggttcagata ccctgtaccg acatagtttc ctaatcgata gccttcaacg
     8761 ccgaatcttg cgtatttttg cgctaatttt gaacaatcag ttacgttcac agctaactct
     8821 tctactttgc ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc
     8881 atattcttcc ccgtccatct tccacatact tcatatcatt tcacatgata cttatgctct
     8941 catcatacca caggttgaat tagaaaagaa aataaatcac acaaaaaagc aagacaatct
     9001 gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa aactggttgc
     9061 ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat cttccagtgc
     9121 atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct cgttttgagg
     9181 gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta atgcttcttg
     9241 tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt cttccattac
     9301 atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag cgtcttctcc
     9361 tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg aaacacccat
     9421 gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat tcacattgtt
     9481 cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat agcgcaattc
     9541 agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa taatatgttt
     9601 tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg
     9661 atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg cataaatccg
     9721 gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt tacgtcccat
     9781 attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct gttccattat
     9841 acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct tctcattttt
     9901 agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga atattgcctc
     9961 ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc tctttttttc
    10021 cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt ccagcatcgg
    10081 taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct tgctttttga
    10141 tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat ctttttgttt
    10201 ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg
    10261 attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta gaagataaca
    10321 accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa gttgaaagat
    10381 cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct aacgcatggc
    10441 gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga tagcctgcac
    10501 ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt tcctttgcat
    10561 aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg ggatacagac
    10621 tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac agttcttgaa
    10681 atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa
    10741 acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt
    10801 ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg ggaagctcca
    10861 ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta tcgatgatag
    10921 ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc tctgctcctg
    10981 ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga ttgtactcta
    11041 cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa aaaagaaata
    11101 gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa actattctcg
    11161 caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg
    11221 atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg caaaaccgct
    11281 agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa
    11341 aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt aaataacttt
    11401 ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga ccagaccctt
    11461 cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg cttcctggat
    11521 aactatccca caaaaaagca aaattatccc cttgataatg atagctatat tctggaaaaa
    11581 cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt tcttctaacg
    11641 cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata tagcacagcg
    11701 aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg agcatattgc
    11761 cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca atcatcaaca
    11821 tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag gcaaccgcta
    11881 atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt aaatgacgtt
    11941 tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg taaatgaaag
    12001 caaatgtttc ataagccata tgttaatcca accattcact aagatcgact agcggagcat
    12061 ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa acatgtacga
    12121 tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca atacgtttta
    12181 cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg ttgatttgac
    12241 gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca tttcgaacgt
    12301 ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca atttttaatt
    12361 gttctttact atctatgatg atttcctcct aatatgagca gcttattttt ttgctaccca
    12421 gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa gcaaatgggc
    12481 caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg caatggctcg
    12541 tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta cataaatgat
    12601 tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg catgaagaat
    12661 ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa gaccatgcca
    12721 gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga ttaccatttt
    12781 gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa tactagcttt
    12841 ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta ctccaagcac
    12901 atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt tttctctaga
    12961 ataagcagta tattcattac tatagtccat attcatacaa cccttcattt tgaatataat
    13021 caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc ttttgacgta
    13081 ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga tatggagtat
    13141 ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc agctcatcga
    13201 ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag taataatctg
    13261 tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt ttcccaccac
    13321 gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct aattccaaca
    13381 tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt ggctgataag
    13441 aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca gccatcacta
    13501 aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc tttttcttct
    13561 ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg
    13621 tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt tgatactttt
    13681 cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg atttcgcaat
    13741 ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta ttttgaagta
    13801 acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg atcattgctg
    13861 aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct ttgctacgta
    13921 aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa atcaatgctt
    13981 tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact ggcgctgtta
    14041 cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa
    14101 aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt aataatccac
    14161 ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga atatttacat
    14221 catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca aatcttgcaa
    14281 gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt ttgatttctt
    14341 ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt tgatggcgat
    14401 ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc tcgattttat
    14461 ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc gctgtatttt
    14521 tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg atatagacat
    14581 cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca tgagatttct
    14641 tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca cgttccttca
    14701 tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact ttgttcccaa
    14761 ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt gaaccattga
    14821 agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat tcattcagta
    14881 agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt ctcattataa tggcgtaatc
    14941 tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa gccgtttgcg
    15001 gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag ccgatacatt
    15061 gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt ttgctaatag
    15121 ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac tatctgttgc
    15181 gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac gtatatcagt
    15241 cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt gtttctttgc
    15301 tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa gatcaaattt
    15361 ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa cagggattcc
    15421 tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg gattattcca
    15481 tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat tctttaattg
    15541 ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat ttgaaaacat
    15601 gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa ccccgtatta
    15661 tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga aaaagcattt
    15721 ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac aaaaaacaac
    15781 taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc taatcgaaca
    15841 agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt atatcgtggc
    15901 aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt
    15961 tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc gtctttccac
    16021 aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc cagcatccag
    16081 acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc aatgatgatg
    16141 gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat agcctctcct
    16201 tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg ctgtattgat caaggtaatc
    16261 accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg atagccttca
    16321 ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc tttttgaata
    16381 ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat cgcttcgtca
    16441 tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa
    16501 tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg tgctgtctta
    16561 tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga ttttctgttt
    16621 caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga attcagcctt
    16681 ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa
    16741 accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa aactggcagt
    16801 tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt cattgctatg
    16861 ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc cttcttgcat
    16921 ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg ttgctcgttc
    16981 gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga taaaattagg
    17041 atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag tgaggactgc
    17101 ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt cgggaaatgc
    17161 tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt catcaataat
    17221 caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag ggcatacttt
    17281 ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact ttgcccacat
    17341 gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat tgatccgaat
    17401 atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt ctggtgtcca
    17461 attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg
    17521 atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg tgataaattc
    17581 acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat ggacgattat
    17641 ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc
    17701 tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc ctggagaagg
    17761 agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt gtccttggaa
    17821 tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca cctgtttttt
    17881 tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga ttcgagtatt
    17941 catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg ttccggcatt
    18001 ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt ctctttttgt
    18061 ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc gttgtaaaga
    18121 acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga tctgaacttc
    18181 tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc caaatgccgc
    18241 tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg attgaacttt
    18301 ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat aaccaattct
    18361 gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc ctggaatgac
    18421 tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca ttgcatcgat
    18481 cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg aggcaaatcg
    18541 gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg tcacgattgc
    18601 agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg ggccaatgca
    18661 gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg aatatatcgc
    18721 tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga tttcaccgcg
    18781 gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag gttaattccg
    18841 cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg gctgaagctt
    18901 tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact ttcttacgga
    18961 attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa
    19021 gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct ggcataacag
    19081 gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt ttcttagcaa
    19141 cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt ggataacgat
    19201 gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc tgctttgctt
    19261 atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg ttttctacaa
    19321 ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt ttcatcgctt
    19381 caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg aaagctggtt
    19441 tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc tcgattccat
    19501 caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct tgttcaggta
    19561 tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata ttcatgcctc
    19621 gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa tcgaattccg
    19681 ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata tccaaaatta
    19741 ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta atgaattact
    19801 cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca agtcaatctc
    19861 aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta atgccagcaa
    19921 agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca tcgatttcgt
    19981 gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg cttttgcttc
    20041 tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga cttgttccgg
    20101 ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag aaccatctgg
    20161 agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga tttctccata
    20221 gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa cgcctgctcc
    20281 ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac gattaggatc
    20341 agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct cacaaatcgt
    20401 tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt tacggaatgc
    20461 ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac aaataccttt
    20521 tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca ttggtacgaa
    20581 cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt tttcgattgt
    20641 tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat tcattttttc
    20701 tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat agatcgaaaa
    20761 catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga aatctttgac
    20821 ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc
    20881 attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt
    20941 gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt cttatccgtg
    21001 catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat tttgtgctaa
    21061 gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt
    21121 ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg tgatgaatcc
    21181 tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca cagattttgt
    21241 taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat ttccaatcaa
    21301 cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca tttttttcaa
    21361 tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt ttttgaaatc
    21421 ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt ttactaacac
    21481 atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt tctcataatc
    21541 agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt cttctatctt
    21601 ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt ttgcaaaagc
    21661 taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat tttttccttt
    21721 tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt ctgcttattc
    21781 tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct taatactttt
    21841 ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg gatactctca
    21901 tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc
    21961 attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa ttgaatctga
    22021 cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg
    22081 ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa aacagcttcg
    22141 tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata atttgcagga
    22201 gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc aatcggcgca
    22261 ttcatcactg caaccatttt cccgcttcca gctggggcag cttctgtcat ataagcgcct
    22321 ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc tactaatgcc
    22381 gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg ctccttcaat
    22441 aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg cgtctgatcc
    22501 aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat ctcacttgct
    22561 tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat accttgatat
    22621 tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa tatatccaga
    22681 atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca ggaaatgtcc
    22741 cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag tcactaaatc
    22801 ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat cttcaatttc
    22861 attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga tgtcagtagt
    22921 caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt g
//
LOCUS       DADXTG010000034        22725 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.034, whole genome shotgun sequence.
ACCESSION   DADXTG010000034 DADXTG010000000
VERSION     DADXTG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.034"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            48..458
                     /locus_tag="IWI57_001124"
     CDS             48..458
                     /locus_tag="IWI57_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBC2449283.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            451..1152
                     /locus_tag="IWI57_001125"
     CDS             451..1152
                     /locus_tag="IWI57_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBC2449284.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1152..2738
                     /locus_tag="IWI57_001126"
     CDS             1152..2738
                     /locus_tag="IWI57_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449285.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2757..2978
                     /locus_tag="IWI57_001127"
     CDS             2757..2978
                     /locus_tag="IWI57_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBC2449286.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2975..3736
                     /locus_tag="IWI57_001128"
     CDS             2975..3736
                     /locus_tag="IWI57_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBC2449287.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4116..4625
                     /locus_tag="IWI57_001129"
     CDS             4116..4625
                     /locus_tag="IWI57_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBC2449288.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4700..5239)
                     /locus_tag="IWI57_001130"
     CDS             complement(4700..5239)
                     /locus_tag="IWI57_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBC2449289.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5379..5990)
                     /gene="rpsD"
                     /locus_tag="IWI57_001131"
     CDS             complement(5379..5990)
                     /gene="rpsD"
                     /locus_tag="IWI57_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBC2449290.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6252..7103
                     /locus_tag="IWI57_001132"
     CDS             6252..7103
                     /locus_tag="IWI57_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBC2449291.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7131..7766
                     /locus_tag="IWI57_001133"
     CDS             7131..7766
                     /locus_tag="IWI57_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBC2449292.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7786..8559
                     /locus_tag="IWI57_001134"
     CDS             7786..8559
                     /locus_tag="IWI57_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2449293.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8967..9764
                     /locus_tag="IWI57_001135"
     CDS             8967..9764
                     /locus_tag="IWI57_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBC2449294.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9742..10155
                     /locus_tag="IWI57_001136"
     CDS             9742..10155
                     /locus_tag="IWI57_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBC2449295.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10139..12784
                     /gene="mutS"
                     /locus_tag="IWI57_001137"
     CDS             10139..12784
                     /gene="mutS"
                     /locus_tag="IWI57_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBC2449296.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12802..14910
                     /gene="mutL"
                     /locus_tag="IWI57_001138"
     CDS             12802..14910
                     /gene="mutL"
                     /locus_tag="IWI57_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBC2449297.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14932..15492
                     /gene="maf"
                     /locus_tag="IWI57_001139"
     CDS             14932..15492
                     /gene="maf"
                     /locus_tag="IWI57_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBC2449298.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15577..17565)
                     /gene="treP"
                     /locus_tag="IWI57_001140"
     CDS             complement(15577..17565)
                     /gene="treP"
                     /locus_tag="IWI57_001140"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBC2449299.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17788..18501
                     /gene="treR"
                     /locus_tag="IWI57_001141"
     CDS             17788..18501
                     /gene="treR"
                     /locus_tag="IWI57_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBC2449300.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18578..19024
                     /gene="msrB"
                     /locus_tag="IWI57_001142"
     CDS             18578..19024
                     /gene="msrB"
                     /locus_tag="IWI57_001142"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBC2449301.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19194..19796
                     /gene="ruvA"
                     /locus_tag="IWI57_001143"
     CDS             19194..19796
                     /gene="ruvA"
                     /locus_tag="IWI57_001143"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBC2449302.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19809..20810
                     /gene="ruvB"
                     /locus_tag="IWI57_001144"
     CDS             19809..20810
                     /gene="ruvB"
                     /locus_tag="IWI57_001144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBC2449303.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20839..21417
                     /locus_tag="IWI57_001145"
     CDS             20839..21417
                     /locus_tag="IWI57_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2449304.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21469..21951
                     /locus_tag="IWI57_001146"
     CDS             21469..21951
                     /locus_tag="IWI57_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBC2449305.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22068..22442
                     /locus_tag="IWI57_001147"
     CDS             22068..22442
                     /locus_tag="IWI57_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449306.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7395 a         4067 c         4919 g         6344 t
ORIGIN      
        1 aaaggtttgt tagtaaaatg aaagctatac gtaaggagtg ttgtttgatg aaattttttc
       61 aagagttttt cattattatt ttcttttctg tattaggaga aggcatacgg atttttgctc
      121 atttacctat tcctggaagt atcttaggta ttcttttttt atttttagca tttgaaatgc
      181 atcttgttga tcctgaaaaa atcggagcaa ctggtgattt tttattgaat aatctagcca
      241 ttttatttgt tcctgctggt gtaggtttat tggaatattt tgatgatatt gcttcgatat
      301 ggcctgtttt actgggagca gtcgtcgtgt gttccatcgt cacgatggtt gcagcgggaa
      361 aaacggcaga aggtgtagaa gctttgctgg gctatgtccg taagaaaaaa gcagaaaggg
      421 tatctgtaaa cgaaatggag gagaatcaaa ttgactgagt tttttgtgaa tccattattt
      481 ggcttaagtt tgactttagg agtgtacgta ttatctgaca aatttttgcg gaaatataaa
      541 ctgcctgttt tgaatcctct cgtattatcg attgttgtta ttatcttatt tttgcatttt
      601 tctgggatca gctataaaga ctataatatc gggggcaatt ttttgactat gctgattact
      661 cctgcgacag tggcacttgc catccctctt tataaaaact tccgtttact gaaagagaat
      721 ttttatcctg taatggctgc gattttagca ggaatagtcg caaattgctt ggttagtgta
      781 gggttcggtt acttcttctc tcttcataaa gagatggtga tctcattact acctaaatct
      841 gtaacgacgg ctatttctgt cgatttatct catacgatgg gcggggtgaa tgccgtgaca
      901 cttgctattg ttgtctctac aggtattttt ggttcgctga tcgccactca tattttccgc
      961 ctattcaaga tcgaaagtcc agttgctcgc ggtgtggcgt taggaagtac ttctcatgct
     1021 attggtacag caaaagcaat cgagattggt gaaatagaag gaatcatttc cggacttgcg
     1081 atatgcgtca acggaatatt gaccgtttta ttgctaccgt tattctttca gccttttgct
     1141 ggcttgtttt gatgcgaaaa ccactatttt atgctacaat aaccaaaacg ttcgagcata
     1201 aaaaaggagg gaaagacgtg tatttgtctt ccgagtatct taaacaattt gataaagatc
     1261 tatattataa gattctcctc ctagaaagtt ttgaggatca agcttggcat accgctgcac
     1321 aattagcgca agttgttcag ctggatgccc gcagcgtgtc gaaatacctg aacgagctgt
     1381 ctaaaaacta tcagcagttt agcggtaaga cacacccttt gtttacaaaa aatcaccgtt
     1441 ctggttataa cttttatgat acgctcgatt caattgaaca tgagcgtttt ttgatctatc
     1501 tcgtgcaaag tacgctgaaa ttccagttgc tgcacgacat tttttttgaa gaattccata
     1561 caatgtacca atttgcgcaa aagcattaca ttagtgaatc tactgctcat cgaaaaatca
     1621 atgaatggaa acagcaactg caaacttacg gaattcggct gcaacgaggt acatatatcg
     1681 ctcaaggcga agaagaaatc attcgacttt acttgcatat gacattctgg cagttgtttc
     1741 gagggaaaat ctggccattt gaaacgattt ctcaaatgga cgtaaagaat atggcagaac
     1801 atatcatggc tttttttaat gttcgtctga acgagatcaa aaaaagacga ttggaatata
     1861 tgctaggtgc ctttttttta agaaaatcac agaagcatta tgttgtattg aatgagaaaa
     1921 aaagacggct gatttctgac aatctgttgt tccagcgatt ttgccaggtc atggagccgg
     1981 tatttcctaa ttattttcaa gtagaagacg agttaggcgc attatttctt gtactgatga
     2041 ctcgtgaaga atattatagt gatcctaaga tacggaagaa aatttttgat tttcatcaac
     2101 aagcaaaaac accgcccttt acagcccttt cagaagcaaa agccgctctt tctctttatc
     2161 aggaagagca ggggctgcca gcagaaaatc taaccttcga ggcagaaaac tatttattca
     2221 gcagtcattt tttcgcctat ctttttccta atgcgaaaga aacgattgat ggcaatagta
     2281 gtgattttat taatcattta gttattgaaa acaaggaatt gaaacaatgg ctcgtacatt
     2341 tttttgaaag tagacacaaa catcccaatc atctggcatt taaaaatcat gcgtttttga
     2401 tggggcggta tctgactgtg tttaaaacat taggagcctt tactccacag cttccaaaga
     2461 tcacgatcct tttaatgaca gatttcccat tatttgaaga gcaactgtta gaggagggat
     2521 tacggacatt tttccgtaat gagtatcagc tgatttttct gccaacagat tatcggggga
     2581 gagaagttga tcttctgatt tcgacaagta aagtccaccg caaaccatgg gcggatcttg
     2641 actattttat tgtgactgaa gaactaaaat taattgatta catccaacta tctcaaaaat
     2701 ttgaaatgat tcaaaaacaa aaactattaa aacaataaca aagaaggaga aaaaagatga
     2761 catttgaaga aattttacca gaattgaaaa aaggtgcaaa gattattcgt aaaggatgga
     2821 gtggatttga actttatgtt gttctcgtat ccgatgatga atttgatggc tgtccggtga
     2881 cgccgtactt attgatcaaa acaagtgacg aaggtttttc cagcttcgct cctacagttt
     2941 gtgatatttt agcagatgat tgggcgatcg tagaatgaaa aaccagtatc cagatctagc
     3001 taaacaggtt gtatttatca caggagctgc ttctggaatt ggtgctgcgc aagcccgagc
     3061 gtttcttgaa caagatgctt ttgtatttgg attagatcaa aagttcggta aaatggaaga
     3121 aatttctttg cgatttcctg aaacttttgc ctacgcacta ggtgatgttc gacaaatgaa
     3181 agaactccaa caagcggtat cgaaatgcca aaatcatttt ggtgaagtta cgattttact
     3241 gaatacagcg gggatattgg atgcctacaa acccttgatg gaaactgacg aatcgctgtg
     3301 ggatctgatt tatgagacga atgtgaaaag catgtatcag ctgacaaaaa tcatccttcc
     3361 tgatatgatc cgccaaaaaa gcggaacgat catcaatatg gcttcgattg ctggcttgat
     3421 tgctggtgga ggtgggatcg cttatacaag cgcgaaacat gcgattgtcg gatttaccaa
     3481 acaactcgct ctggattacg caggacaggg tatccgagta aaaggaattg ctccaggagc
     3541 catccaaaca ccaatgaatg cggctgattt tgaagggaaa ggtgagatgg cagagtgggt
     3601 ggcaaatgaa acaccagtca agcgatgggc acagccggaa gaagtagctg agctgacgtt
     3661 atttcttgcc tctcctcaag caagctacat acaaggaacg atcatcccaa tcgacggcgg
     3721 atggttgtta aaataacctt gtgttttttt caagcttctg tatgataaaa ctaaagagag
     3781 aaacaacggt tgatgataaa aaaagtcgat gactgttccc gacattcgat gccgaacagc
     3841 cagttcaaaa aattcttttt aataatcgtt aatattcgaa actaagcggc ttttcacgac
     3901 ctcttgatat aaacgatatt caaaagcttg caactgcggt aacaagtcca gccaaatgaa
     3961 aaatataagg agctattttt atttgaccat tcttattact tgttgcaggg cagctttttc
     4021 actaccttct gcgacacttc agttggaatt ttcttcccat cctttggtcg cggtcattac
     4081 caagataaaa ttatcttgca aaggagaaat agaacatgga aaaaaataat acccgttcag
     4141 ccaaacgctg gacaacgaca gatgtgactc ggatggcgct agtcactggt ttatatgtgg
     4201 cagtgacgct ggttttgtct gtctttagct ttggagtgat tcagattcgt ctctcggaga
     4261 tgttcaacta tctgtccttg tataacaaaa gatacatcgt cgcggtaaca gcgggagtag
     4321 ctttggctaa tattgcttct cctcttggac tgatagatgt tgcagtagga agcgtcagca
     4381 cgctgcttgt attgcttatc aactataaaa tcactagtcg catcaaaaac atgaaaatca
     4441 aaatggccgt gacagcatgc gtatttgctt tttccatgtt tacagtagct ggtcagctaa
     4501 cgattttata tcaactacct tttttcttga attggtgggt gattgcttta ggagaattgg
     4561 catcaatgat tgtcggtggg ataattattt attggatcgg taaaaaagtg gatttgtcaa
     4621 aataaagatc tgtttaattt aaaatagggc agcatggaag agactgaatg ttgagtcttc
     4681 ttccatgctg cttttttgat taatttgtat gaagaaatcg atttagccgt tttttcatca
     4741 agataccgat ataagctgct ccagttgcct tgattgcttc cggcagtaaa aacggcagta
     4801 gtccactggc aaatgcagtt gtaaaagtaa gatcaccact tattttcagc cagatcgttc
     4861 cgcagatcaa tgcagcaaga gagcccatta gattggcaaa gattgcatat aagtaatgag
     4921 catccgttct ttcgatcatc caaccagtta tccacgtttg taagagaaat ccaatcaaat
     4981 atccgccagt tggcccaaat aaaacagcta tacctccagt catccctgaa aatacaggaa
     5041 gaccaatcaa gccaagtaaa agataaactc caacagatat caatcctagt tttcttccga
     5101 gaaaagttgc tgttaatcca atagcaaatg tttgccctgt caaaggaacc aaaccaaaag
     5161 gaatggtcat ctgagaaagg acagaaataa tcgcggcaaa ttccgcagca agtatcaatt
     5221 ctttcaatgt tcggttcata acgccctctt ttcgttaact taatattttt atttggttaa
     5281 cgaaattgta aaacaaagtg attactttgt aaatagaaaa tataaaaaaa gaccagctat
     5341 ccaatagata gcggtctttt tcaagaaagc ttttgaaatt ataagctttg gttgtagtat
     5401 tcaacgacaa gagcttcgtc gatttctgga tatagttcgt cacgttctgg taaacgagtc
     5461 aatgaacctt ccaatttttc agcgtcaaag cttacgaatg ctgggcgtcc aacagttgct
     5521 tctacagctt cttttactgt tacgatgttt tgtgattttt cacgaactga gatcacttga
     5581 cccacttcta cgtggtatga agggatatca acgcgtttgc catcaactaa aatgtgacca
     5641 tggttaacta attgacgtgc ttgacgacga gtcgttgcaa gtcctaaacg gtaaacaaca
     5701 ttgtctaaac gttgttctag taagatcatg aagttaacac catgtttacc ttctttgatt
     5761 ttgctagctt ttacgaacaa gttacggaat tgacgttcgt tcattccgta catgtgacga
     5821 agtttttgtt tttcagttaa ttgcatgccg tattctgatt gtttgccacg gctattaggt
     5881 ccatgttgac ctggtgcgta agggcgacgt gctaattctt ttcctgtgcc tgacaatgag
     5941 atgcctaagc gacgagaaat tttccatgat ggtcctgtat aacgagacat taaaatttcc
     6001 tccaatgaat ataagttttt ggagtaaaat aatccgttga aaaattcata atcatatagt
     6061 ttgttcttca atcttcacct ttgcagccgc ggttacacaa tcgaacccga gacggggcaa
     6121 caaactaggg atgagttatt atttttctgc tgcattattt tacacaaatt acagtatatg
     6181 atagatcatc cacgaatgtc aagataatta gtacataaaa acgctttaca aacctaggct
     6241 gtctttataa aatgagaggg tatgtgcaaa tggaaaaaag cgaaggaagt ttaaggagaa
     6301 aatcaatgaa tccggtatca ccattatttg agaaaatcga tgcttcaatt gaaaagaagg
     6361 cgaatctttt tgaaaacagc ttttcacgtt acgctgttcg agcggttctc gcctgtttgt
     6421 ttttgacttt aggaacggct gtggcatttg cgatcgcgat gaagggggaa gatatcgcgc
     6481 atggtctagg aaagatttta tacgcattta tgttcagctg gggtctggtc atgattctat
     6541 acatgaacgc cgaattagga acatcgaata tgctttatat gaccgtgggt gtttttcggc
     6601 agagacttgc tttaagtaag gcagccaaga ttctatttgc ctgtattttt ttcaatatga
     6661 ttggcggtat attatgtgga tttctaattt ctatgactac gccatttcaa gatttaacgt
     6721 tggacaactt tatgttagaa tcaatagcag taaaactagc aaaaccttca gcaacgattt
     6781 tggtggaagg gatgtttgcg aatatcgttg tcaataccgc tgtactgatc agcatgcgga
     6841 tgaaagagga tgtcggaaaa gtgatcacag tcgtattcat cattttcatt tttgcttttt
     6901 taggatatga acacgtgata gcgaattttc ctgcctttac gcttacttac tttgcttctc
     6961 acggacagat ggatggaatg acagtcagca atgtgttaca taatttgttt tttgctttgg
     7021 caggaaatta tattggtggc ggtttagtaa tgggactggg atatgcttgg ctgaatcagt
     7081 caaaatcatc ctatgtggac taaaaagtcg ttcattaaag gggaagtgcc atggaagttt
     7141 tagtttttcc aaaaaatgag gaaaaagcgt tacaaatgga aaagtacatg aaaaatcttt
     7201 tttcctttgc tggagtacct aagccggaac gcgctctttt acaacaaccg ttactggatg
     7261 agagcaaaaa tttatctatt tctgaagttt tagcttttgt tcattattat tatgaacaaa
     7321 tcgaaagaga gtatcagtat gtagcgatcg atttagtcca gaaaaacatc aaacggctta
     7381 catttgaaga cttagtcgta ttgttgccac tagttcagaa aaaagaatgg tgggacagca
     7441 tcgactcttg gagaaaagta tacagcacat ggtgtaaagc ctacccagag cagcgtgaag
     7501 aagtctttca attgtttgaa aaaaaggaag atttttggct acgtcgaata gcaatcacgt
     7561 tgcaattagg ttataaacag caaacagaca caggactatt gatccgagca atagacgaag
     7621 atcgtcaaac aaaggaattc tttatccaaa aagcaattgg ttgggcatta cgcgattata
     7681 gtaagaccga tgccgattgg gtaaaaagac agctgacaac agatttgagt aagttagctg
     7741 ttcgagaggc aagtaagtat ttataagaaa gcgagacgac aagagatgag acgcaaatta
     7801 tttgcttttg atattgatgg aacgttgtta gattcaaaca aacaagcttt agatagcaca
     7861 agagaagcat tggaaaaatt acgcaaagcc ggtcatctgg tcacgattgc tacagggcgg
     7921 agccgatttc atgcgcaaga gatcattcgc gatcttgatt ttacgaacta cattttatgt
     7981 aatggagcgg ccggtttttt agaccatgaa caagtatata agaatttact agatcgcgac
     8041 cagttggatg cgttcgttca agaagcgcat catgagcaga tcgatacagc ttttgtcagc
     8101 ttagacaata tcaagcgttc ttcttccaat gatgtaacgg tgatggaaga agcgatgcat
     8161 tctttcggtg ctgtcttgcc tgaattagat cagttttttg cagaggaaca agaaatatac
     8221 caagcgttag catttttcga tcaaacatat gatgggaagt tctcttcata tgataaaatt
     8281 cgatttgtcc gctggcatga gaacagtgta gatgtcgtac ctaaaaatgg ttctaaagca
     8341 gcgacaattc tgcacttagc agaacaagta ggcatccctc atgaagacat catcagcttt
     8401 ggcgatggac aaaacgaccg tgaaatgttg cgcatgtcag gaattgggat agctatgggg
     8461 aatgctgtgc cagaagtaca agcagaagct aagatggtca ctgatacaaa cgatcaggat
     8521 ggaatttgga aggcattgaa agagttaagc gtgatttaaa agaggttgtg aaaaagctgt
     8581 tctgcatcaa gtaataagcc cagccaaaca aaaatagctc ctcctatttt tcgtttttct
     8641 gggcttatta ccgcagatgc aagctttcga acaccgttta aggaagaggt cgtgacagaa
     8701 gtggtcagtt tcaagaaata agaagggatt tacgaaaatt gcttctcaaa tttttgtaaa
     8761 attacggctt atttctgaag aagtagatga aacaacgttt attcgggaaa tgggactgtg
     8821 acaagatgtt tgtcatagtc ccattttttt attttatttt tcaaaactaa gaaataccag
     8881 gaaagagttc cttttgtttg accttttctt tcttattgaa aacaactata atatagagtg
     8941 atgttaaaag agaagaggaa tttagtgtgc gggtattgtt tattggtgat gtagtaggtt
     9001 caatgggacg agagatgatt actgaatatc ttcctagatt gaagaagaag taccgtccgc
     9061 aagtaacgat tgtaaatggt gagaatgccg cttctggacg tggaatcaca gaaaaaatct
     9121 ataaaaaatt tttgcaagac ggcgtagatg tcgtgacgat ggggaatcat acgtgggaca
     9181 accgcggaat ctttgagttt gtaaatgaag ctaaaaagat ggttcgtccg gcaaattttc
     9241 cggaagggac gcctggacaa gggatggtct ttgtcaaagt gaaccaagtc gaactggcag
     9301 tgatcaacat gcaggctcgt tcttttatgg tggacttgga tgatcctttt cgtaaaatga
     9361 atgagctggt agaagaagcc agaaaacgta cgccgatcat ttttgtagat tttcatggag
     9421 aaacaacaag tgaaaaacaa gcaatgggct ggtttttaga tgggaaagtt tcagcagtgg
     9481 tcggtacaca tacacacgtt cagacaaatg atgcgcgaat tttgccaaga ggaacagcgt
     9541 atctgacaga cgttgggatg acaggtccct atgatggtat tttgggtatg cgcagagaac
     9601 ctgtaatcga aaagtttctg actgcattac caaaacgttt tgaagttgtg gaacaaggcc
     9661 gatcgatttt aagcggctgt atcttggaat tggatgatac gacaggtcat gcaaaagaaa
     9721 tccagctgat tcagatcaac gatgatcgac cgttcatgga gtagaaagag aggcagtttt
     9781 ttgcagcagg agccggaaat tcaaaaagaa gtaaggaaac tgacaaaact tttaagagag
     9841 aatgaaacga tcattcgcta caaagaactt gaagaaaaga tccagcaaaa ccagtattta
     9901 gctgagttga gagaaaaaat caaacaagcg caaaaagatg ctgttcattt tgcccactat
     9961 gacaaaccag cagcagaaaa agaagcaatc aaacaagcgg atcaattcat gcaagaattt
    10021 gatcagcatc cgcttgttgt agcttataga aaacagctgt tggaagcaga tgatctgctt
    10081 catcatttaa caactatgat acaagaagaa atcaatggac aaatagagga ggaaaaacat
    10141 gcctcaaaaa actaaaaata ctcctatgat ggaacaatat ctaagcatca aggcccaata
    10201 ccaagatgct tttttgttct atcgtttagg agacttttat gaactatttt acgaagatgc
    10261 gatcaaagcg tcacagcttt tggaattaac gttgacgagc agaaatcgaa atgcagaaga
    10321 acctattcct atgtgcggtg tacctcacca tgcagcacaa ggatatatcg atgccttgat
    10381 tgaaaaaggc tataaagtag cgatttgcga gcaggtagaa gatccaaaga cgactaaagg
    10441 gatggtcaag cgcgaagtga tccagctagt cactcctggt acagtcatgg acagcaaagg
    10501 attgtcggca aaagacaaca attttttaac agctgtagtt agtcaaggaa atgaatttgg
    10561 ctttgcttat gccgatttaa gtacgggaga attgaagacg gctcggttgc atgatgaaga
    10621 agctgtttta aatgaagcct ctgcattgca gacaaaagaa ctcgtattag gcagtgagat
    10681 cactgatacc cttcgtgaac agctaagcgg gcgtttaggg ctagtctttt ctcaacaaaa
    10741 tgagatggaa gaaaacgcgg aatttagctt tttgacaagc gaactagtcg atccgctgga
    10801 aaaagaagca accggcaaac tattgactta tttatcggtg acacaaaagc ggagtttagc
    10861 tcatatccaa aaagcagtag aataccaacc agatcatttt ttgaaaatgg attattattc
    10921 aaaattcaac ctagaattaa gccaatctat tcggactggg aaaaagcacg gcactcttct
    10981 atggttgtta gatgaaacaa aaacagcaat gggtggccgt ttactaaaac aatggctaga
    11041 ccgtccgctt atccaggcaa aacaaattaa agcaagacaa gaaatggtcg cttctttgct
    11101 agacagctat tttgaacgta tcgatttgca gtcagcatta acgaaagtct atgatctgga
    11161 acgcctagca ggtcgagtag ctttcggtaa tgtcaatggg cgagacttga tccagctgaa
    11221 aacttcttta gaacaagtgc caatgatcag agggttgatt gaaggaatcg atcaaggtca
    11281 atggcaaagt ctgctttcag aaatgcagcc gatggaccat ctcgtccaat tgatcgacca
    11341 agctatccaa gatgatccgc cgttacaaat aacggaagga aacatcatca aagatggatt
    11401 caatgagcag ctcgatatgt accgttctgc tatgagaaac ggaaagaaat ggttggcaga
    11461 acttgaagca aaagagcgtc aagaaacagg aatcaagaac ttaaaaatcg gatttaaccg
    11521 cgttttcggt tattatatag aaattacaaa agcaaatctt ggaaatgcag aactggagaa
    11581 gtatgaacga aaacaaactt tagcaaatgc agagcgtttc atcacaccag aactaaaaga
    11641 attggagaca caaatcctcg aagcagaaga aaaatctgtt gatttagaat atcagttgtt
    11701 tttagctgtg agagaagaag tcaaaaaggc tatccagccg ctacaagtac ttgcaaaagc
    11761 aatcagtgca gtagatgtgc tacaaagttt tgcaacgatc agcgaacgct atcagtatgt
    11821 tcggccagaa cttgtcagtg acaagcatca gcttctgatc aaagacggac gacatcctgt
    11881 agtagaaaaa gtattaggac atcaggaata tatccctaac agcgtagaaa tggcagaaga
    11941 cgaaatgatc ttgctgatta caggaccgaa tatgtctggt aaaagtacgt atatgcgaca
    12001 gttggcatta actgttctga tggcgcaaat gggctgtttc gttccggcaa aacaagcagt
    12061 tttacctatt tttgatcgga tttttacccg aattggtgca agtgacgact taattgctgg
    12121 acaaagtact tttatggtag agatgatgga agccaatcaa gccctgcgtt atgctacgcc
    12181 aaacagtttg attttgtttg acgaactagg gcgaggaaca gcaacctatg acggaatggc
    12241 gttggcccaa gcaatcatcg aatatatcca tcgtcatgta caagcaaaaa cactcttttc
    12301 tactcattat catgaactga cggtacttga gaaagaactt ccgcagctta aaaatgtcca
    12361 tgttggagcc gttgaaaaag acggagaagt tgtcttcttg cataaaatga tggatggacc
    12421 tgcggataaa agctacggca tacatgtggc aaagatcgca ggattgcctt ccaatctact
    12481 agaacgagca gcagatatcc tgcatacgct agaaaatggc gataacggac accatactgt
    12541 tccagctgaa gtagaagaga aaactaaaac agccaaacag gagcaaataa aaccacctgc
    12601 agaaaaagta aatcaagacc atatacaaga ccgtgtaaaa gaagaaaacc aacagctttc
    12661 tttattcagt gaattatccg aaaatgaaac agaagtcatt gaatcactga agcaaataaa
    12721 tttactagaa atgacgccaa tggatgcact aaacacatta tatgaattac aaaaacaact
    12781 gtaggaagaa ggcggatatc gatggccaaa atccaagaat tatctgaacg tttagctaat
    12841 cagatcgctg cgggagaagt agtggagcga ccagcttccg ttgtgaaaga attagtggaa
    12901 aatgcaattg acgccggcag tactcagatt gatatcttag ttgaagaagc gggactgcgg
    12961 acgatccaag tgattgataa cggagaaggc attttggcag acgacgtaga gaatgccttc
    13021 aagcggcatg cgacaagtaa gatccataat cgcgacgatc tttttcgcat acgaacgctg
    13081 ggctttcgag gggaagcttt gccaagtatt gcctctgttt ctgaaatgac tctggaaacg
    13141 gcgaatcagg aagaaaaaca aggcacctat ctgtcgctga aaggcggaga agttattgag
    13201 catcgaccag caccactgcg tcaaggaacg aagattaccg tcagcaatct gtttttcaat
    13261 acgccagccc gcttgaaata cgtgaaaaca ctgcacacag aattagcgaa catcggggac
    13321 atcgtcaatc gactggcatt gagtcatcca tcgattgctt ttcgtttagt acatgatgga
    13381 aacaaaatgc tggcaactgc tggaaatggc gatctgaaac aaacaattgc gggaatctac
    13441 ggattatcta ccgcaaaaaa aatgctgaaa atcgaaggaa aagatctaga ttttgagtta
    13501 tttggctatg tttcattgcc tgaggtgact cgtgccagta ggaattactt atcgacgatc
    13561 atcaatggac gttttatcaa gaacttttcc ttgaataaag caatcgttgc aggttatggg
    13621 tcaaaattaa tggtcggtcg tttccctatt gctgtattgg aaatcaaaat ggacccgctt
    13681 ttagtagatg taaatgttca tccgacgaag caggaagtcc ggctgtcgaa ggaaaaagaa
    13741 ctaacagagc taattagtaa cgccattcaa aaagcattgt ctcaagaaaa tctgattcca
    13801 agtgcagcgg ataatctacg gtttaagaaa aaagtacctg atcagcctaa aacagaacaa
    13861 atcgaaatcg atttgtccta cgcttttgaa gaagaaaaac ctaaaaagcc aagtagtttg
    13921 aattttgatc gagagacagg tacctttttc gtagaagaac taaagaacac atttgttgac
    13981 agaattgttg ataagtctgt ggaaaatgtt gaaaactatt ctggtttttc tgaagaaaac
    14041 accacttatc cagaaaaaac agcgaaaacg gtggataagt ttggtgaaaa tgttgataac
    14101 tttgtagaaa aaacagtgga aaaatctgaa aagaaagaat ttcccattgt ggataacatg
    14161 gagaaaacaa atgaagaaca agcagataaa tccgtagaag aggatgaaaa gatttacgag
    14221 gaagaattga cactccatcc tgaatttgat ttcactcaag gaaacgacaa aacattagaa
    14281 caagccatca agaaattaga aacggaaaaa ccagtacaac gttttcctca gttggaatat
    14341 tttggacaga tgcacggaac gtatcttttt gcccaaagta gcgatgggtt gtacatcatt
    14401 gatcagcatg ctgcccaaga gcgcattaag tatgaatatt tccgggaaaa aattggggaa
    14461 gtatctgatg atctgcaaga attgcttgtt ccatttgtgt tagactatcc aaacagtgat
    14521 gctttgaaat taaaagagca aaaacacttg ctagaagatg cagggattta tttagaagaa
    14581 tttggtcaaa acagctttat tgtacgggcc catcctactt ggtatcccaa tggagaagaa
    14641 gaatcgatta ttcgggagat gatcgatatg ctgctgacaa ctggaagtgt gagtgtaaaa
    14701 aaattccgtg aagcaacagc aatcatgatg agttgcaaac gatcgattaa agccaaccat
    14761 tatttgaatg aagcacaagc acgtgtgctg ctgaaggatc tggcacgatg cgaaaatcct
    14821 ttcaattgtc cccatggccg tccagtattg attcacttta ccaattcgga tatggaaagg
    14881 atgttcaaac gaatccaaga cccgcattaa gtcaaagaga agagggaaaa catgaagatt
    14941 gtattagcat cccaatcacc aagaagaaaa gagttattgg ctcatatcgt ttcagaattc
    15001 gagattgaac ctgctgatat cgatgaaacg ccatttccag acgaacagcc cgtcgattat
    15061 gttcgtagaa tggctgaaga aaaagcacga actgtctggg aacaagatga aaaagaagac
    15121 catttagtga ttgccagtga cacgactgtt gttctcaatc aagagatcat gggtaaacca
    15181 gaagatctgg ctgaggctga attcatgctg aaaaaactaa gcggggaaac acatactgta
    15241 tatacagctg ttgttttaaa aacaaaggaa cgagaagaac ggatactagc agaagcacat
    15301 gtcacttttt atcctttgac agacgaagag atcaaacgct acttggagac gggggattat
    15361 gcggataagg caggggcata cggcatccaa aatgctgcga gtgtattcgt caaagaaatt
    15421 tctggagatt attacagtat cgtaggattt ccgattggtg ctgtcaatca ggcactgaaa
    15481 acattcattt aaacaaaaaa gcggactatc tgattttcga ttgaaaatcc atgatagccc
    15541 gtttttttta ttgaaaagat tacttatcta ctctgactaa gattttactt cagctttacc
    15601 gactgcttcg cccgggcgaa caagtcctga ttggtcaaga gaaagtcctg caactttttc
    15661 attattggtc aaagcaacaa taatgtcagt tttcttgcca gcttgttcta tttctggcaa
    15721 atccatttca gcaatcaggg tctctggtgt gacaaggtat ccttctttca cgaatacatt
    15781 gaaaggaaca ccctttagtt caacagtatc taatcccatg tgaacaagta cttccagccc
    15841 ctcattagaa agaataccaa tggcatgttt tgtttcaaag acactcgtca ctttaccgtt
    15901 tactggtgca tagactttgc cgtttgacgg ttcgacagca taaccgtcac ccatcatttt
    15961 ttgtgagaaa actgggtcat ttacttcagt tatttccttg atggtccctg cagcgactgc
    16021 gaataatgtt tcttttgttg caactgccgt accagttgct gaaacaggtt cgaaagactg
    16081 tggcgttgct gttgcgccat ttgctgtttg aagttgtaca tcagcagcat tttcaggaac
    16141 agcaggatca atcttattca aaattccctg acgtctaaag atgatcgtca agccaaatgg
    16201 aatgatgaca gcaatgatca ttgcgatgat gaaaggtacc catgtttctg cttggatagc
    16261 tagtattcct ggcaaaccgc ctactccgat cgcgttagct cgaacaccca tcaagttagc
    16321 aaacatgcca gctaatccag agccgaccat tgcagcgacg aaaggataga cgtatttcaa
    16381 attgattccg aacatagcag gttcagttac gcctaagtaa caagaaatca ttgctggaat
    16441 cgagatttgt tcttcttttt tattcccacg atgtaagaag atgatggcta agactgcaga
    16501 gccttgggcg atatttgata aagcaatcat cggccataaa ttcgttgatc cgaaatctgc
    16561 aatcagctgc atatcgatgg cattggtcat atggtggaga ccagtaatca ctaaaggagc
    16621 atacaggaat ccgaatacgg cactaaacaa ccagctgata gaagacgtca atccagtatt
    16681 tacgatattt gagatccagc ttccgactgt ccagccgata ggacctaaaa taacgtgtgc
    16741 agctaaaaca gttggtaaca atgaaaacaa tggaacaaaa atcatcgaga tcgactgagg
    16801 gatatatttg cggaagaaga tttctagata cgctaacata aaccctgcca gcatggctgg
    16861 aataacttgc gcttgataac caatcatctg gacttgagca aaaccaaaat cccaaaatgg
    16921 aatatctgct gcggctgtgg aagctacact ataagcattt aataattgcg gagaaactaa
    16981 cgtaattcct aaaacgattc ctaatatttg tgtagtaccc atcttttttg caatactcca
    17041 agtgatacct actggaagaa aatggaaaat tgcttcaccg ggtaaccaca aaaaggcatt
    17101 gacaccattc caaaacttcg agtgttcaac gattgttcca ccaagacttt caaactggat
    17161 accttcaagt acatttctga atccaaggat caatccaccg acaatgattg caggaatcag
    17221 tggtgtaaag atttctgcta aaacggcaat agcacgttga acgggatgaa ggttttgttt
    17281 tgcagctgct tttccttgtt ctttggaaac cccttcaact ccagatactg caacgaagtc
    17341 gttataaaaa gtagaaacat cgtttcctat gatgacttgg aactgtcctg cattcgtgaa
    17401 catccctttt acgctaggta tatcttcgat cgctttttcg tccgcttttc ctggatcatt
    17461 caggacaaaa cgcatacgag tcgcacaatg actaactgca gcaatatttt cttttccgcc
    17521 gatatcatgt aacagcttct cggcatctgc ttgatatttt gccatatcaa taaacccctt
    17581 tctcttaact tgtctatgca agttgtttct tcatttacta tacttttttt cggagagaat
    17641 tgcaagcgtt ttattaaaaa gtacgagaga aaatgatatc gttttaaaaa gcttaaaata
    17701 agcttttctt aaaaatagga acattgaaat acaaaaagag ctgtagtata gtgaagaaat
    17761 gatggaagaa cgagtgaggg aaaaaacatg aataagttca atgaaatttt tttagatttg
    17821 gaacagaaaa ttttagcgaa agaatatcct ccgcattcgc ttttacccag tgagaaccaa
    17881 ttgatcaaaa tttatggtgt ttcacgagaa acaattagaa aagcgctgaa tcttttgact
    17941 acggaaggtt atattcaaaa aaaacaagga aaaggttcga tcgtactgga tcggaatcgc
    18001 ttcgactttc ctatttctgg attgactagc tataaagagc tacaggagac ccaacgtatt
    18061 ccaagtgaaa cgatcatcca tacgttagaa gaaacagaag tgacaaaagc attgaacgag
    18121 atcactggct gggagatcgg tgcgcctgtt tggcatttga tacgcgaaag aaaaatcaat
    18181 ggcgaagtag ttatattaga caccgattat ctattaaagg aaatcgttcc tattttaact
    18241 ccagcacaag cacaaggatc gatctatgaa tattttgaaa atgagctttc tttgacgatt
    18301 gcttacgcgc aaaaggagat cacagtagaa gaagtaacgg aaatcatgaa aacagcgatg
    18361 gatcttcacg atgacactta tgcagtagta gtcagaagcc tcgttcatct ggaagatacc
    18421 cgctgtttcg agtattctga gtcggttcac cgattagata aattccgttt cgttgagttt
    18481 gcccgacgcc gaaaagtgta aatagttcaa aagaaagcaa attgttggca tctgtccact
    18541 ttggcgctat gataggatca caaagggggc atttttaatg gataaaccaa taagaccaga
    18601 aaaagaagaa ctgaaagaaa agttatcacc catggcttat gcagtcacac aagaaaatgc
    18661 aacagaacga ccatttagcg ggaaatatga tgatttttac gaaaagggga tctatgtaga
    18721 tatcgtcagc ggtgaaccgt tattcagctc tgcagaaaaa tatgatgcag gttgtggctg
    18781 gccatcattc agcaaaccaa taacgaaaag acaagtgcgc gaaaaagccg atttttctca
    18841 tggcatgcat cgaatagaag ttcgaagcaa agaagcagat tctcatctcg gacatgtgtt
    18901 taccgatgga ccagtagatc aaggtggtct acgttactgc atcaattctg cctcactgaa
    18961 attcattcca tatgaaaaga tggatgaatt aggttatggg gaattcaaat cacttgtaga
    19021 ataatggaaa tacacaaaga gcggtacaat acggggattt actcgatcgt ccgctctttt
    19081 tttattcaag cgattgctat ttgaaagaaa cgctccctag actccttttt tcacaaggtg
    19141 aaatgtgcta caataaaggg aacgtatgtg tgcaaaagaa agaggactag aatatgtatg
    19201 aatatctgac aggtgtggtt acctttatca acccctatta catagtagta gaaaccaatg
    19261 gtatcggcta ccagatcgca ctaggaaatc cttaccgcta cagtagcaaa ctaaatcaaa
    19321 aaataaaaat ttatgtccat caggtaatac gtgaagatgc gcatcttctg tatggatttg
    19381 attcgctaga agaaaagcaa ctgtttttgc gattagtaag tgtttcagga atcggtccga
    19441 aaagtgccct tgcaatcatg gcaagcgaag accactccgg tttgattcaa gcagtagaat
    19501 ccagtgatgt cacttatttg acgaaatttc caggagtagg gaaaaagaca gcacaacaaa
    19561 tgatcttgga tctaaaagga aaattcggtg agctgagtat cgatacacct tttagtctat
    19621 ttgatgaagc aaacacaaaa gacgctacag ctctatctga agcaatggaa gctctctcgg
    19681 ctcttggtta cagtgatcga gaaatcaaac gtgtagaaaa acaattaaaa gaagtagata
    19741 atcagacaac ggatgagtat ctacgccaag cactcaagct aatgatgaaa aaataaggag
    19801 gagaccaaat gacagatgaa tacttgttat caccagaatc tggcgaaaat gaactgagtt
    19861 tggaaaaatc tcttcgacca cagctgcttt ctcaatatat cggacagaga aaagtcaaac
    19921 aagaacttca gatttacatc gaagctgcca gaaatcggga agaaccttta gatcatgttc
    19981 tgttgtacgg gcctcctgga ttaggaaaaa cgacgatggc catggtcatt gctaatgaga
    20041 tgaccgtaaa tattcgtacg acgagtggcc ctgctatcga acgtccgggt gacttagtag
    20101 caatcttgaa tgaactggaa gcaggggatg tcctgtttat cgatgagatc catcgattac
    20161 ctagagtggt agaagagatg ctttattcag ctatggaaga tttctatgta gatatcatgg
    20221 taggacaagg gccaaccgca catccggttc atttccctct tcctcccttt actttgattg
    20281 gcgcgacgac acgggcaggt atgctttctg cacctttacg agatcgattt ggcatcattt
    20341 ctcatatgga atattatgac gaacaggatc tgcaggagat cgttttacgt tcttcggata
    20401 tttttcaaac agagatcatt gaagaagggg cattggaaat tgctcgccga tcacgaggaa
    20461 cgcctagaat cgctaatcgc ttgctcaaac gggttcgtga ttttgcgcaa gtccaaggag
    20521 acgggaaaat cgatcgtcct atagcggatc aggcacttac tttgctacag gtcgatcagg
    20581 ctggattgga ttatgttgac cagaaattgt tgcgaacaat gatcgagtta tatggcggcg
    20641 gaccagtagg gttaagtacg ctttcagtaa acataggaga agagcgtgag acggtagaag
    20701 atatgtatga accgtatctg atccaaaaag gatttttaaa gcgtacacct agagggcgaa
    20761 ttgctactgc ttatgcctat gaacattttg gttacgacta tcatatatga tttttgcttt
    20821 tagattggag gaaaatgaat gagtgataca acacgtacga aacaattgat caaaaacgta
    20881 ttgattgatt tgtgccaaac gcaatctttt cgaaaaatca gcgtccaaaa aatcgcaaac
    20941 gaagcaaaaa tcaatcgaca aacattttat tatcatttta cagataaata cgatttgctt
    21001 cgttgggtct attgtgagga tgcattgcat tatttagatc cacaatgtct ttcacttgat
    21061 aattgggaag agcaagcatt gaaaatgctg aaagcaattg ctgagaagaa agaattttat
    21121 gcaaatactg tgctttctga cccagaaatt ttacaaaaag agttttctca actgatccaa
    21181 aaatcattta tcaagatttt tgaacaaatg gatgaagaaa gacaactttc tgcaagagac
    21241 aaactatttt atgctcgttt tttttcttac ggttgcagtg gtgtattagt caattggatc
    21301 acgaatgaat atgaagaaac accgatggaa atcgctatgc agcttttccg tttagccaag
    21361 gatacggaat tatattctta tcgtctttat gcaaaagaag agttggaaga acagtaatag
    21421 aaaaattttt tgttggaaaa gcaataatcg ctaaaaggag atggggaaat gacaaaaatt
    21481 ttatttgttt gtttgggaaa tatttgccgt tcaccgatgg cagaaggctt gctgagaaaa
    21541 cgaatagcat tagaaggaag ggaagcagaa ttttttgtgg attctgctgc tacaagcacg
    21601 tatgaagttg gaaagacacc gcaccctgga acgaaaaaaa tattaaacca agaaaaagta
    21661 gatatgacga atatggttgc tcggcaaatc acaccccacg attttgaaac ttttgattgg
    21721 atcattggca tggatcaaga aaatgtagaa gaactgaaac gaagagcacc acgctcagcg
    21781 caggggaaaa tccatttgtt tttaagtagc attcctggaa aagaaaaaga aaatgttccc
    21841 gacccatact ataccaataa ctttgatgag acatatcact tgatcacaga aggcctgaat
    21901 cattggtgga atatttggat tgatgaaaat aaaataacaa acaatttgta aagttaacgc
    21961 tttcttttta aggcgatgtt agagcatttc cttgtttttt tgtaaaatgt taggtaaaat
    22021 aaaattaatt aagtaacact ttcttagcta aaggaggagt gcattcgatg aacgaagaga
    22081 cagtatcgcc aatgaagaag cttacgaagt tagttactca tacttcgtat caactttttg
    22141 attcttttgt aaatgacact gaagaacatg ctaagaatgt acgattgcaa ggaaagttta
    22201 ttcttgagct gcaaaaagct gctactgaac atagtctagt tatcttacaa gtaagagcaa
    22261 atcgtttgag tagcaagtat gaaacgatct caggctgggt ggcgacaaaa acggtcgaaa
    22321 aagaccgagt gatgatccgc ctacaaaacg aagagcagca gatccggatg gttcctgtag
    22381 aacaaattga aaaaataaca atcctttcac cagcagggga acaggaacgg ttatcaaaat
    22441 aatttatacc agatagaaaa gcacgtatga gcttgtacca tcaatcatta ctaatcaaaa
    22501 ataaccgaat gctttaagct gatatcagta aaacggaacg ttttaggatg agtatcatag
    22561 catgacagca ttcggttgat tagttgaatc gattggatgg tacaagtttt tttgataaaa
    22621 aaaggtcctg ctagccgggg agctagcagg ataaggagtt aaaaatgaaa aagtgttgtt
    22681 agggttgttt gttggtatgc ttatataata gtcattagtt gtgaa
//
LOCUS       DADXTG010000035        22617 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.035, whole genome shotgun sequence.
ACCESSION   DADXTG010000035 DADXTG010000000
VERSION     DADXTG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.035"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            189..2402
                     /locus_tag="IWI57_001148"
     CDS             189..2402
                     /locus_tag="IWI57_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBC2449307.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            2413..2859
                     /locus_tag="IWI57_001149"
     CDS             2413..2859
                     /locus_tag="IWI57_001149"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBC2449308.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            3030..4289
                     /locus_tag="IWI57_001150"
     CDS             3030..4289
                     /locus_tag="IWI57_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBC2449309.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(4378..5325)
                     /locus_tag="IWI57_001151"
     CDS             complement(4378..5325)
                     /locus_tag="IWI57_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBC2449310.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            5719..7011
                     /locus_tag="IWI57_001152"
     CDS             5719..7011
                     /locus_tag="IWI57_001152"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBC2449311.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            7026..8795
                     /gene="aspS"
                     /locus_tag="IWI57_001153"
     CDS             7026..8795
                     /gene="aspS"
                     /locus_tag="IWI57_001153"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBC2449312.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            8904..9779
                     /locus_tag="IWI57_001154"
     CDS             8904..9779
                     /locus_tag="IWI57_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2449313.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            10045..11928
                     /locus_tag="IWI57_001155"
     CDS             10045..11928
                     /locus_tag="IWI57_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBC2449314.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(12003..12287)
                     /locus_tag="IWI57_001156"
     CDS             complement(12003..12287)
                     /locus_tag="IWI57_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBC2449315.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(12277..13011)
                     /locus_tag="IWI57_001157"
     CDS             complement(12277..13011)
                     /locus_tag="IWI57_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBC2449316.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            13140..13763
                     /locus_tag="IWI57_001158"
     CDS             13140..13763
                     /locus_tag="IWI57_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBC2449317.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            13955..15076
                     /locus_tag="IWI57_001159"
     CDS             13955..15076
                     /locus_tag="IWI57_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBC2449318.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            15083..18211
                     /locus_tag="IWI57_001160"
     CDS             15083..18211
                     /locus_tag="IWI57_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBC2449319.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            18230..21424
                     /locus_tag="IWI57_001161"
     CDS             18230..21424
                     /locus_tag="IWI57_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBC2449320.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(21570..22277)
                     /locus_tag="IWI57_001162"
     CDS             complement(21570..22277)
                     /locus_tag="IWI57_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBC2449321.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         7621 a         3994 c         4795 g         6207 t
ORIGIN      
        1 atagtcaaca atttgtataa tagaaacaat tatttataca atggatacga aaaatcgttt
       61 gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg tataactgca cggattttgg
      121 gacaattatc aaacagatat ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat
      181 cgattcctgt gacaaaagaa gaaattttaa cgggacccgg tgtcataaag ctcgttagtc
      241 aatacatggg tcccgaacat gtagcatttg ttgagaaagc atgcgaatac gcgactgctg
      301 cacatgacgg acagtttaga aaatcaggcg aaccctacat tattcatcct atccaagtcg
      361 caggtatatt agcagattta aaaatggatc cccatacagt ggctacaggc ttcttacatg
      421 atgttgttga agatacagaa atcactttag aagatctgag agaagaattt ggcgatgacg
      481 ttgctatgtt agtggacggc gtaaccaaat tagggaaaat aaaatataaa tcccacgaag
      541 agcagctagc agaaaaccac cgaaagatgc tgctagcaat ggctcaagat ttacgagtta
      601 tcatggtcaa attagctgat agactacata acatgcgtac gttgaagcac ctgcgagaag
      661 ataagcaaag aaggatcgct caggaaactt tagaaatcta tgcgcctctt gcacatcgtc
      721 tagggatcag ccggatcaaa tgggaattag aagatacagc acttcgttat ctaaatccaa
      781 aacagtatta ccgtatcgtc cacttgatgc agacgaagag agaagaacgt gaaaaatacg
      841 taagtggtac tgttgaagat attcgaatag cgacagaaga gctggggatt tttgcagaaa
      901 tctatggacg gccaaaacac atttattcaa tctatcgtaa aatgaaagat cagaaaaagc
      961 agttcaacga aatttatgac ttgctagcta ttcgagtgat cgtagattca atcaaggatt
     1021 gttatgctgt attaggagca atccacacaa aatggaaacc aatgcccggc agatttaagg
     1081 attatattgc catgccaaaa gctaatatgt accaatcttt gcatactacc gtgatcggtc
     1141 cggcaggaaa tccggtagaa attcaaatcc gaacacagga aatgcatgaa atcgctgaat
     1201 tcggggttgc tgcacactgg gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg
     1261 gtatgacgaa acaattaagc tggttccatg agatactcga acttcaagac gaaagctatg
     1321 atgcttctga atttatggaa ggcgtaaaag gagatatctt tagtgataaa gtctacgtct
     1381 tcacaccaaa aggagacgtt actgagttac caaaaggatc cggaccattg gactttgcat
     1441 acagtatcca tacagatatc ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg
     1501 tgcagcttga ttacaaattg aaaaacggag atatcattga gatcatgact tctccaaatt
     1561 catttggccc aagtcgcgac tggttgaaat tagttgctac tagcaaagca agaaataaga
     1621 tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt gatcaaaggc cacgaatccg
     1681 tggtcaaatg tattacagat ctaggattta cgcctaaaga tattttgacg aagaacaaac
     1741 tgcaagaagc actcgatcgt tttaattatc aaacagaaga tgatctctat gcagctgtag
     1801 ggtatggaga agttagcccc ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag
     1861 aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat catgaatcag ccgaaaaaag
     1921 aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact
     1981 tactaattcg tatcagtcgc tgctgtaatc cgattcctgg tgatgatatc gttggttata
     2041 tcactaaagg cagagggata tccattcatc gtcgagattg tccgaatgtt cagcctgaca
     2101 aaccaaatgt agcagaacgt ttgattgaag tcgaatggga agatacatcg aatacacgaa
     2161 aagagtatga tgcagatttg gaaatttacg gctataatcg ttcaggctta ttgaatgatg
     2221 tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag cgttgaagca cggacgaata
     2281 aagataaaat ggcgacgatc catttgacgg ttggtatcca gaatttatcc cacctaaaga
     2341 gtatcgtgga taaaatcaaa gcagtacctg atgtctacag tgtacgccgg acgaatggat
     2401 aggaggacag atatgagagc agttgttcaa cgggtaagta aagctgctgt gtcagtcgat
     2461 cagcaagaaa ttggaaaaat tggctacggc ctacttgttt tattaggcat tcatgaaacc
     2521 gatacgcaaa aagatgtgga ctatctggta aaaaaaattg cacaaatgcg tatttttgaa
     2581 gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg gaggacaaat tttaagtatc
     2641 tctcaattca cactgtttgc ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc
     2701 gcacgaccag aaacagctat tcctttatac gaatcattca atgaaggaat cagaagtcgc
     2761 ggcatcacag tcgctaccgg acaattcgga gcagatatgt cggtttctct cgtcaatgat
     2821 ggacctgtaa cgatcattat tgattctaaa aacaaataaa gtaatctggt tttcactagt
     2881 aaaaactatg aaacacgtcg cttaagttag actgataaac tctaacttaa gtggcttttt
     2941 tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa aagttaagct taaattatac
     3001 aagttatttt ttataggagg ttgctgaaga tgaaagaagt accagtagta gatatcaaaa
     3061 tcacagattt aaaaaaattg tatcaaaaag gatacaacat gttggaggag ttacgccatg
     3121 aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa agaagctatc acgatctacg
     3181 gatcatcagc agcaaaagta ttttatgatc caaggaattt caaacgaaaa ggtgctatgc
     3241 caaaattagt attgaagaca ctttttggtc aaggtggagt acagactttg gatggagctg
     3301 ctcatcacca tcggaaaaat atttttatgg atctaatgac accggaaaga atggaagatt
     3361 atcatcgtat cttagataag aatctgacac aagcacttga agcacagcat ggtcaatttg
     3421 aactatttga tttaagtaag atggttttct ttacatctat ttgcgagtgg gcaggtatca
     3481 atttatcagc gatttctaaa gatgaggtcg aaaaattagc agaatatcaa atctctatga
     3541 tcagcggaac gtttacttct ccgattgatc acataaaagg tgtcgaaaat cgcaaaaaat
     3601 cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca aaatcctgtg gctggaaaag
     3661 aaaatgtggc attatatgct tttgccaatg ccacagattt agatggtcag cttttacctc
     3721 tggaagtggc agctgttgaa ttattgaaca tcatccgtcc aacggttgct ctaactgtgt
     3781 gggcggcatt gatgggacat gcacttttca gtagaccaga tttgtatcag caattaaaaa
     3841 atgacttttc gacgttacaa gatccgttta ttcaagaaat gagacgctat tatcctttct
     3901 tcccaatgct acctgcgatt tcgctgaaag aagtagaagt agatggttat cgtataccag
     3961 aaggcagttg ggttatttta gatttatatg gaacagacca tgatgaacga acagttgaag
     4021 cgccagattc ctttatgatc aaacggtatg tagggaaagc aaaagatatt tcctataaag
     4081 aagaatatga gatgattgca caaggcgggg gaaattttcg acaaatgcat cgctgtgcag
     4141 gagaatggat cactcttcat agtctacgtg tgttttccga tcagctagta aataagtttg
     4201 agttctctgt acctgaacaa gattggacga ttccattcaa tcagtttccg acttatccga
     4261 atagccgagc gctactttac aagaattaga ttagcataaa cttatcgaaa aaaaggaaga
     4321 tcaaacagtg ctgatcttcc ttttttcata ttactataaa ttgataaggt caattgatca
     4381 aaatccacga aatgcttcat caatggtttg ataatcttca ttggataaat gcaaatctaa
     4441 agcttggaca ttgtcagata cttgctctgg acgcttagct ccaggtataa ctacagacac
     4501 tcgtggattc ttcatgtacc aagctaaaac aagttgcgca ggagtggctt ggtatctttt
     4561 agcaatgggg cgaagtacat cgacagcggt taaaatttct ctgaaacgtt ctccttggaa
     4621 atctggattt ctttttcgag gatctccttc accaaattgt ttttcctcac caagttcata
     4681 ttttcctgtc agcaaaccgg aagcaagagg aaaataggga acaaaactga ttttgttttt
     4741 ttcaagataa gggaataatt ctttttcagc ctgccgatgg atcaaactgt atttatcttc
     4801 tactacatcg acatatccat ctgcattcgc ttctttcaat tgttctaacg tgaagtttga
     4861 cacaccgact gctcggattt ttcctgcttc ttttaattcg tgcaatgtag caactgattc
     4921 attttttggt gtgctttcat ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg
     4981 cagacgtttt agtgcatctt ccaccgattg tttgagaaac tcaggagaat tatcaaatgt
     5041 tcgcccttta tttggtacat gagcagcttt tgtagcgatg acgacacgtg agcgatcata
     5101 ttctttttcc ttcagtacct cgccaatcag ttcttcggaa cggccgtttc cgtaagcata
     5161 agcggtatcg atcaaattga tcccgctatt caaagctgta cgaactactt gttttcctgt
     5221 ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg gcatttgctc ctaatccaag
     5281 ctgttctgcg tagacttgtg aatgtccgat tcttatttct gccatagctg attcctcctt
     5341 tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa
     5401 tagtcagata tctcttgact ttgagactgg tttttagtag gttataagta atatcgaatg
     5461 tcaatgaaag agcgagtaag tttttgaaac tttacaagag aggatcatcg aggctgaaaa
     5521 tgatctaaag tttcattagc ctaaagacac tctggagatg gacgagtgaa aagccgtccc
     5581 gcgtacgagc gttattcgta agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt
     5641 atgaatttca ttcacccttg ttcatgatga acaagggtga tttttttatt tacatagcta
     5701 agaaagggag agaaaactat gagtttccaa agaccaaaag gaacaaatga tattttgcca
     5761 ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac gtttattatt cagagattat
     5821 cagtataatg aaatgcgtac accgattttt gaacactatg aagtgatatc ccgaagtgta
     5881 ggggatacaa cggatatcgt ttcaaaagag atgtatgatt tttatgacaa aggggaacgc
     5941 catgtgacgc ttcgaccaga aggtacagca ccaatcgttc gttcttttgt tgagcataaa
     6001 ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt atatgggacc aatgtttcgc
     6061 tacgaacgcc ctcaaaaagg acggttacgt caattccatc aaatcggagt agaagcattt
     6121 ggatcagaaa accctgcaac tgatgtggag agcatggtga tggcacttga tttcttcaaa
     6181 cagttgggga tcacgcaaat ccggctagtc atcaattcat taggtgataa agaaacgcgt
     6241 caagcctatc gtcaagcatt gatcgactat cttttacctt ttgaatcaga gctgagtgaa
     6301 gactctaaac gccgccttca tgaaaatcca ttacgcgtat tggacagtaa agacaaacga
     6361 gatcaaaaat ttgtggcaga agctccatct atcttagatt atctaagcga gcctgctcaa
     6421 aaacattttg ataccgttgt ttccatgctg gatgcattgg ctgttcctta tgaaatcgac
     6481 agtaatatgg ttcgcggact agattattac acacatacga tttttgaaat catgagtgac
     6541 gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg gacgttatga caatctagtg
     6601 gaagaactag gtggtccttc aacacctggc ttcggatttg caatgggtat cgagcgatta
     6661 ctcttgacta tggaggcaga ggaagtagtt atccctgcat ttaatgagtt ggatgcttac
     6721 gtggtcgcac tcggagacga aacaaatatc gaagcattga aagtggtcca agcgattcga
     6781 aactttggtt tttcagctga ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag
     6841 acagcagata aagcgaatgc caaattagta ttggtgatcg gtgaagatga gcttgctagt
     6901 caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa aaagttttcc attagaagag
     6961 atatatgaac attttgatga agtctatgat gaaatgactt tattcgagta ggaggaaaaa
     7021 aataaatggc acaaagaacg atctattgcg gattagtttc cgaacaacaa ttagaacaaa
     7081 cagttacatt aaaaggatgg gtacaaaaaa gacgagacct tgggggagta atctttatcg
     7141 acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag
     7201 catgggaaat cgcggataaa tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta
     7261 gaagacgcgg agaaaatgca gtaaatccta aaatggccac tggtgaattc gaagtgattg
     7321 caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc
     7381 aagcaatcag tgacgaaata cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa
     7441 tgactaaaaa catcaaatta agaaatgcga caacaaaagc gattcgtcat tatttagatg
     7501 ataatgactt tctcgatatc gaaacacctt acctagggaa atctacacca gaaggcgcaa
     7561 gagactacct agttccttca cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc
     7621 ctcaattgtt taaacaattg ctgatgaatg ctggattcga ccgttattat caaatcgttc
     7681 gttgtttccg cgatgaagat ttacgtggag accgtcagcc tgaatttacg caagtagata
     7741 ttgaaacaac gttcttaaca gcagaagaaa tccaaactta cacagaggga ttgattgcga
     7801 aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc atttcctcgt atgacctatg
     7861 atgaagcaat ggctcgctac ggcagcgaca aaccggatac acgttttgat atggaattga
     7921 tcgatctaag cgatacagtc aaagaagtag aattcaaagt cttccaaatg gctcttgaaa
     7981 acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag
     8041 acatggatca gttaggtcaa tatgtaggac aattcggagc taaggggctt gcatggctga
     8101 aagtagaaga agacggattg aaaggtccaa ttgctaaatt catgggagaa gcaacagaag
     8161 caatcattaa agcaacagat gcaaaaccag gtgacttgct gatgtttggc gcagataaaa
     8221 gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg tttaggaaaa gaattaggac
     8281 taattgatga atcgaaattc aatttcttat gggtaactga ttggccacaa tttgaatttt
     8341 cagaagaaga aggtcgatat gtctctgctc accatccatt cacgatgcca aaagaagaag
     8401 atatcccgtt attggctgaa gaccctgcaa aagtctatgc agaagcttat gatatcgtct
     8461 tgaacggtta tgaactaggc ggaggttcta tccgtattca tactcgtgag cttcaagaaa
     8521 aagtactaga aactctaggc tttacaaaag aacaaatgga agaacaattt ggtttcttgt
     8581 taacagcttt agattacgga ttccctccac atggcggtat tgcattagga ttggaccgtt
     8641 ttgcaatgtt attagccgga gaagataata ttcgtgaagt cattgccttt cctaaaaacg
     8701 gaaaagcagc agatgtgatg agtgatgcac caagtatggt ttcgccttta caattgttcg
     8761 aattgaatat tgatgtaacg aatatagaag aatagccgat aaaaaggtaa aaaggatttg
     8821 tcgaatacgc ttcttttgac aaccttgatt taattgaaaa acactttata atgaagccat
     8881 aacatataaa gcggggcttt tccatgaaaa aaacattcga aaattgggct tatcatgatt
     8941 acacgaccaa gctttcggta tctatcgttt atgcgttgat cgcatcagtc gcattgaatt
     9001 tcttttatca gccaggacat atttattcaa gtggtattac tggtctagct cagattttga
     9061 cgactttgtc aaaatcactg ataggattcg aagtaccggt atcaacaact ttgtatttac
     9121 tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg gaagaagttt acaattttca
     9181 cttttatcac agtcactttg acctcccttt gtatccatat tttaccggaa acagtcctat
     9241 ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt gatgggacta ggtattgggc
     9301 tagcgttgag aaacggccta tcatcgggcg gtctagatat cgtgagtatc acgattcgta
     9361 aaaaaacagg aaagtcgatt ggttctattt cgatctactt caatgcattg atcgtttttg
     9421 ttgccggaat tctatttggc tggcaatata tgttttacag tacgttgtct attttcgtca
     9481 gtggaaaagt aactgatgtc gtttatacta aacaaaagaa gatgcaggta atgatcgtaa
     9541 cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga
     9601 tcatcaatga agcagaaggt gcttataagc atgataagca gacagtattg atgacgatcg
     9661 ttacacgata cgaactgcct ctacttagag aagcaatgaa agcttctgat ccacaagctt
     9721 ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt ctatgaggat ggcatttaga
     9781 tcgagtttga caaaaattca tgaaatactg ctgtagacag acgttcgttt gtctgcagtt
     9841 tttttctacc atagtagaat cagcaaagaa aaactgcttt tttatttgaa attttcttaa
     9901 agttgaaaaa aagcatgttt atttttttaa atttatggga tgatagaaaa aaatagtaga
     9961 gaaaggacac gattctactt gctattttca cttaataccc gtatattacg aatgaaaaaa
    10021 gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac
    10081 atcactgggt ttgatgggct ccgttcatta gctgtggtgg gtgttatctt ctatcacctt
    10141 ctgccaacta gtatgaaagg cggctatttg ggcgttccga tttttttcgt cgtatctggt
    10201 tatttgatca cggatctgct gcggcaggaa tgggaacaaa atggaaaaat caatatttgg
    10261 caattttatg tcagaagaat gaagagactc tatccggcac ttgtttttat gcttgtgaca
    10321 gcttcggctt atatcacttt gtttcagcga gggctgttaa acaatcttcg aggagttgtg
    10381 gtcagcagct tactgtatgt caacaactgg tggcaaatca ataacggttt atcttatttc
    10441 gatcgatttg ctaatgagtc accgtttacg catatctggt ctttagcagt agaaggtcaa
    10501 aactatttgg tttggccgat cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa
    10561 tggatctttt atactgtgtt aggtacatcc ttcctctccg ccatcttgat gatgatcttg
    10621 tatacaccag gaggcgatcc aacccgtgtt tattacggta ccgacactcg tctgttttct
    10681 atatggttgg gaagtgcgct tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc
    10741 ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa tctcgctttt ggcactgatt
    10801 ctttgcttct tctttttaga tgatcattac aatttcatct attacggagg aatgctccta
    10861 gtgagtctgt tatgcgtagt gctagttgca gtgacagcac accccggagc aagtttgaat
    10921 cgatggctga caaatccagt atttacttgg attggcaaac gaagctatgg catttatctg
    10981 tatcagtttc cagttatgat tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt
    11041 ttgttgcata ctttagtgga aatcacattg attctaatca tcagtgaact ttcttatcga
    11101 tatgtggagc gtccgctagc acgttttgat tatggtcgta cattccaagt gatgaaagga
    11161 tggttcagca aaccgatcat cagtaagagc aaaccatggg ttgttccagg cacattactt
    11221 gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa
    11281 aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag cagaagaaac cagaaacaat
    11341 tcagaaaaca ccactagttc ggttgaccaa gcgatccttg ataagtacga gttgtccaag
    11401 gcacaaggga ataaggcaca aaaattagaa ctaacggcat ttggtgattc agttatgttg
    11461 gatgctgcaa cggatctaca agaactttat ccaaaagtag tggtagatgg agatgtcgga
    11521 cgacaattat atgccagtgt accgtacatt gaaaaattaa aggaacaaga tttgttgaaa
    11581 gacactgttc tgatcggact aggaacgaat ggctcattct cggaagctca gtttgatagt
    11641 gtgatggatg caatcggtga tcgaaaagta tactgggtca atgtccgagt accgacgaag
    11701 cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga cgaaaaaata cgacaatatg
    11761 acgctgattg attggtacag ctacagtaat gaccatagtg actggtttta tgatgatcaa
    11821 gtccatccaa atcctgaagg gatgacacat tacattcact tagtcagcga agccttgcta
    11881 ggaggaagtt caagtacgaa cagcagtgag acaaacgtga cagaatgata gctacaaaaa
    11941 acttcaggca aaaacatttg tttttgcctg aagttttttt atatttaccg tgtttttaac
    12001 attcatatag attcaggaag caaaacggtt gagtgtgtct gtaaataacg ttctttttgt
    12061 tttcgggtga gttttttgaa tgcagcttct gcttttgttg cttcgcttct tgaagaaaat
    12121 acttctgcat ggatcatctt aacaggtctt cttttttgaa gacgagtata tttagcacca
    12181 gtaccattgt tatgttcctc taatcgtcta gaaagatcgg ttgtatatcc accataaaat
    12241 gtctgatctt tacagaataa tacgtaaaaa taatgttcat ttgccataga gcatctcacg
    12301 aatctctgca gtgtattctc catccatatt gtaagtgatc aatggtggag cgactttgaa
    12361 tccgtctaat ttgccttctt tgattccttc aactaacaaa atatttgcct cttttccagg
    12421 ttttgggtaa acaaattgga tccttttagg tgcaattcga tagtcttcca tgacctgaat
    12481 gatttctaag aaccgatcag gacgatggac catagtaaag cgtccatttg tttttagtag
    12541 cttacttgaa atatcgatca cttcttctaa cgtcgtatga atctcatgac gggcgattgc
    12601 caggtgttta ttagggttcg ttttatttgt tggcagccct ttgaaataag gtggattaca
    12661 gacgactaag tcacaagaat tatgtcgaac gacagataag ctgtctttca gatcgatggt
    12721 gtggacagtc atttgttcat ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc
    12781 tagacgaggt tgaagttcga tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag
    12841 tccaactgcg ccatttcctg cacaaaggtc aacaatcttc ccttttttag gtacacggga
    12901 aaagtgagca agcagcacag catctaaaga aaaagaaaac actttggaac tttggataat
    12961 cttgatatct tctgcgtata attggtcgat tcgttcgtct tcgtgaagca tggattccct
    13021 ccaaaatgaa aaaaatagtc gtctgtatta tactattttt attaaaaaga accaaggtgg
    13081 cttgtcaaac ctctattttt tatgcatact atgaccataa gaagggaaag gaagagacta
    13141 tgttttatcg atttatgcgc ggggtagttc gagtactatt agccttagtt aatggaaatg
    13201 cccgctatga gaataaagaa gttttaccgc aagatgagaa ttacatacta gttgctcccc
    13261 atcgtacttg gtgggaccca ttatatctgg cggtagctgc agcacccaag gaattcagct
    13321 ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg atttatatta actcgatcca
    13381 atgcttttcc agttaaaaga gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga
    13441 ttttgaaatc gacaaatttg agtttgatca tgtttcccag tgggacaaga catgctacag
    13501 aacttaaagg cggaatggca ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg
    13561 tatatcaagg tccgttgaca cttggggatc tgttcaagcg tcagagagtg acagtgcgtt
    13621 tcggcgaacc aatcgactta tcagatatca agaaaacgga taaagaaggt atggcggaga
    13681 tcgaacgacg aacacaagca gcttttgaca aattggacaa agaagtgaac cctgatttca
    13741 aatatgaagt caaaaaaaaa taaaatgata agtttatgcg tgggcggtct tttgaatcta
    13801 ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg agagacgtca agtctgccct
    13861 ctttttgcgt tccaatatgt aaaatagaag acaggtgtca tgtaatggat caaagtgtta
    13921 taataattag aaggacattt tttgaggtga ttgaatgcgt tttttacata cagccgattg
    13981 gcatatcgga aaaaaactac aaggttatga cttgattgaa gaccagcgtc atgtactatc
    14041 ttctatacta gatatcgcga aagaagaaaa agtagaggca atcgtgattg ccggagactt
    14101 atatgatagg agtgtcccag ctgttgatgc cgtagaatta tacaatgaat tgatggtcga
    14161 ttggaattta aaggaaaaga tcccgatttt tgctatatca ggaaatcacg atagcagtac
    14221 gagactttca gcgggtacgc catggttcag ttattcgaac tattttttgc gaacacagct
    14281 agcagaagca tttgaaccag tcgagtttaa aaacgtacaa tttttctttc ttccttactt
    14341 tgaaccaatt gcagcgagat tatattttga agatgacaat atccgaacga tcaagcaagc
    14401 ggtagccaag gtgatagata aaatgcagga gctatttgat tcagaaaaaa aacaagtatt
    14461 gattagccat ttttttgtaa tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt
    14521 aggcgggttg gattcgatcc ctggtgaact acttacaccg tttgattatg tagcactagg
    14581 acatttgcat aatcaagctg ctttgagaca ggataatgct cgctatagcg gttccccatt
    14641 gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac
    14701 aggcctggag ttgaatttta gggagatcga acccctgcat gatattgaag aaataacagc
    14761 gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc gtcaatcggg agaattacat
    14821 ctatatctat ctcactgata gaacgatcat cccgaatatg atgaaccagc ttcggcaaat
    14881 atatcctcgt attttgggga ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa
    14941 acgaaaagta ctagaaatga aagatcctca aaaattagca gaagcatttt ttagtgaaat
    15001 gacggaagag cagctaactg caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat
    15061 catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta caacttaaaa attttggtcc
    15121 ttttatcaat gagaccgtag atttttcacg tttgacagaa gcaccgcttt ttttgatcag
    15181 cgggaagaca ggagctggca aaacgacgat ttttgatggg atcaccttcg cactttttgg
    15241 tgaaacttcc ggaagacttc gctctggaaa agagatgcgt tctttgtttg ctacaccaga
    15301 cgaagaaaca agcgtgactt tttcttttga acatcagcag atgagctatg aaatcgaacg
    15361 aaaaccagaa caagttttag caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa
    15421 agtaacgttg accatttttg atgggaaagg aaaagaaata aaacaaatca caaaacgtac
    15481 agaagcagat cagttgatca gagaactgat gaatctcgat gcgaaacagt tttctcaaat
    15541 cgttttacta ccccaaggag aattccgtaa ttttttggtt tcatcaagca gtgataaaga
    15601 gactgtttta cgtcatttat tcggtactca atttttccaa cagttcaatg agcgattaaa
    15661 agaaaaagca aaacaacagc aacaaaaatt ggatcattta gagcaagagc tgcatcttct
    15721 gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca gcagggtttg aagccgtttt
    15781 agcccaatgg acaaaccatc aggcaatact cgaaaaacaa atcacggaag aaaaagaagc
    15841 tctaacggaa cttcaggaaa aacaaaagca actagaaaat tcctactatc gttttgaaag
    15901 cttgaaaaaa agttatgaag aaaaaacgat attaataaac aagcaaaagg aattagctgc
    15961 tcaaaaagac gaaatagagg aaaagaaacg atggattggg tattatgaat ttacgcaacg
    16021 attagtcgaa ccaatccagc gcctacaaga aataaaagtt gaaaaaatag aactgtcaca
    16081 aaaaagtagt gagcaaacaa agaaactagc gatgactacg cagaattatg aagagtggca
    16141 aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact
    16201 gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc
    16261 agaacaatta acaatttcat tggataaaga agaattgaat gccgagaagc tgtatcaaaa
    16321 tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa aatttactgg atcaggaaaa
    16381 actgcaagaa gatcgtcttg cttttggaca gttaaaatat ctagatgaaa agcagcaaga
    16441 aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca cagataagag aagaactcaa
    16501 aaaagcaact gaagaaataa caaaaaatag tcagctattg aaagaacaga cagaacaagc
    16561 agaacagctg aaaagccaat gggcaaagca gcaaattgct cgtcttcagc tttttttgcg
    16621 tccaggggag ccatgtcttg tttgtggatc aacggaacac cccatacaac aattaaaaca
    16681 cgaacagcca aataaggaag atatcttaaa agtagaacaa caactggcag aagctgagtc
    16741 tgctgtagaa agaactcagc aaatcattgc caaaagtgaa gcacatgaaa ataatcttca
    16801 acaacaatta tcagatttag aacaaaaaat catggcaacg aatatacaat tggctgaaca
    16861 agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct gagataacag aacaaaaaac
    16921 aaaaacaact cttgtagtaa tcgaacaatc tttagataga cgaaaaagca agatggccaa
    16981 tgcaagaata gagctggaag aaataagaca acaagcagaa aagctcgaac aacaattagc
    17041 tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc cagcaactgc aaggagaaat
    17101 caaaagttta gaacaacaat tgcaagatac ggatagtaac tgtttagaag aggagatacg
    17161 acgacttgaa aaacagttag aagaagactc gaatcaacta gctgttgagc agaaagcagg
    17221 agtgcaattg ttgcaagatc ttcatacgct tcggacacag cttgatcttc aacatgaaca
    17281 aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa agaatagcta aaaagttaga
    17341 aagccagtcg tatttttcaa caattgaaga aatctgtgca tttgcacaac agcaagcaca
    17401 atataaagaa aaccaacgta cagttacttc ttatcatgaa gagcaattag tgacagctga
    17461 ccggctgcgt caattagaaa atatccagct aactgagaca ttcccagata cagaagagat
    17521 cagccaacag ttagaagaaa taagacgtat gatcgaaata aagcaagaaa agatatacgg
    17581 tttacaagaa caaaaaatta gtaatcaaaa aatctacgat gaatgtcttt cgatttatca
    17641 cacaagccag acgaaattag atgagcttac gcaattacag cagctttcgc aaacattcaa
    17701 cggagaaaat cctaagaaga ctagtttgga acggtatgtg cttcaggttt atcttcaaga
    17761 agtgttacaa gtagcaaatg atcatctcca acgtttaacg aaaaatcgtt atcaatttga
    17821 gctagcggat accattggca gttatcgagg gaaaaccgga ttagaaatca atatctatga
    17881 tgatcaggca ggtatgtctc gaagtgcgca taccctgtct ggcggtgaga gttttattgc
    17941 agcactttct ttagcattag ctcttgccga tgtgatccag acacaagctg gtggtgtagc
    18001 aatcgatgca ttgtttattg atgaaggatt tgggtcatta gatgaagaag ctttggaaat
    18061 ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga atgatcggca tcattagtca
    18121 tgtaagagaa ttgaaagaac gagtattaca acaaataaga attgatacag aaggtagcgg
    18181 acaaagcaaa atacgctatc aacttggata aaaagggaga ttggtaacta tgaaatggag
    18241 tattccagaa aaagtcattg cacgtggacg tcaatacttg caagaaggcc gagtgttatc
    18301 ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta
    18361 tatggttgat ttggatggta cagcaaaaga gatagattac tgccaatgtc cctattggga
    18421 ggaacataaa tattgtaagc atacagttgc tgtggaactt tatttacggt cgaaaaatct
    18481 atctcgtatc atgaaaaaag accagccaat gctagaaccg gtaaaagcaa caagtgaggg
    18541 agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa aacaatcctc aagcggtgca
    18601 acctttgatt attgaatacc aagtagaaac gattgagact aatcagtatc acccagagct
    18661 ttctattttg gcactttact tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat
    18721 cgtaaaaaac atctatgatt ttttacaagc ttacacagaa aaagaaactt acactgttaa
    18781 taaacagtac cagtttcgtc tagataaacg ggcgtttcaa aaagaagatc aaaaaatact
    18841 cacgcatctt gcaggaagtg ctcagacgca tcaattatta ggtacgaacg gcttacaagt
    18901 caaaggaaaa ctagataaaa gatatctgct tctcccagta gaacaagccc gcttcctgtt
    18961 agagcagatg aatcagacta cacgtttgca tctgcagata aatgaacaaa acgatcaagg
    19021 aatacaattt tccgatcaaa acaaaccttt atcattcaaa gtggaaaaat ttggagaaga
    19081 ttatttgctg caagcattga atgattttga ttttttcttt atgcattatc agtggggagt
    19141 tatccaaggg acattttatt ctctgacgaa atatcaacaa acggtttatc aaacttggaa
    19201 acaattgatt cgccgattgg agaaaccaga agtagtgttc aagaaaaaag aattaccgtc
    19261 tttgtttaaa gagattatcc ctctgctgtc agagatcggc gacgtagcta ttgcccctgc
    19321 agtagaagag gaagtagcag atatccccgt cgaatttatt ttctttttcc gaaaagcaaa
    19381 aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa gtcatctatt cgactgaccc
    19441 gaagcatgag gtctcgacag aacaggcttt tcaggtgtta cgtgacacag tagaagagaa
    19501 acgagtactt gatctattca gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg
    19561 tccattgcca agcggggaag aactatatgc ctttttccgc acagaactat ctgtcttccg
    19621 tcagtttggt gaagtcagac ttggaagaaa attgcgagag ttgtatttag atgcgcataa
    19681 acatcaaccg aaaatcgaag taaccgagac cggttcatgg ctcgatattc gattcgatgt
    19741 gacagggata gaggagcaag aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc
    19801 tttttataca ttagaaaatg gtcaggttct ttcttttgat tcagaagaat ttcaacaaac
    19861 aagccaagtg ttgcaacaat tacgggaatc gatacgtaca gaagaaggaa cgatccatgt
    19921 acctaaaaat caaggattga tcattcaaaa tcaattggaa aaaagcaatg ctacttttag
    19981 tgaatcgttc caaacgatgg tacaagatct cattcatcca gaaagatacc aggcacagct
    20041 tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag caaggttttc gatggttgaa
    20101 gatgttaggt cattatcagt ttggcggtat tttagccgat gaaatgggac ttgggaaaac
    20161 actacagaca attgcctttt tactttcgga aaaagaagaa cggaaaagct tctctgcttt
    20221 gatcgtagcg cctgcgagct taatttacaa ctggcaggca gaggtgagaa aattcgcccc
    20281 ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa
    20341 agacacagat atccgagtga cttcttatgc cagcttaaga caggatctag ctaattatca
    20401 gtcgcaaaaa attgattatt tgattcttga tgaggcacaa atggtcaaaa atagcagtac
    20461 gaaaactgca caagcattga gagaattagc tgtacctcag cggtttgcac tgagtgggac
    20521 accaatcgag aataatttag aagaattgtg gtcattgttt gcaacgatta tgccaggatt
    20581 tttcccgact aaaacaaaat tcagggaact tgccgcagaa gaaattgcgc aaatgattcg
    20641 accgtttatt ttacggcgag ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga
    20701 gatgaatcta tattctgcac ttacagaaga acaaaaaact gtttatttag cttacttgcg
    20761 tcaaatgcgt gaagaaatca catcaatgga ttctgaagca ttcaagaaaa accggatcgg
    20821 gatacttgct ggtctgacaa gactgcgaca aatttgctgc gatcctcgtc tgtttattga
    20881 agattaccaa ggcggttccg gcaagctcga acaagtaaaa gatctactgg tagctgccaa
    20941 agagaacaaa cgaagagttt tgctgttttc tcaatttaca gggatgctga ctatcttgca
    21001 agaagagtta gccgagctag ggatctcgac attttattta agaggcagta cgaaaccaca
    21061 agatcggtta agtatggtag acgcatttaa tgctggcgaa aaagatgttt ttctgatttc
    21121 cttaaaggct gggggaactg gattgaatct cacgggagca gatacggtca tcttgtatga
    21181 cctttggtgg aatcctgcca ttgaagaaca agctgctgga cgtgcacatc ggatcggaca
    21241 aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga acgattgagg aacgcatgga
    21301 ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca
    21361 gttgacgaaa ctgactgaag aagacatccg catgatttta agtgtaggag aaatcgagga
    21421 gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg
    21481 tttatttgat ttaaaaaata gcaaggaggc tgggattcca ttttatccca gcttccttgc
    21541 tatcattttt ttatttctat ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac
    21601 agctgatcaa aagaagaaat ctcgatatcg caaattggag tctgttcaat tttgcgttct
    21661 cgatggttga accaaagcgc ttgccagtta gcactttttg cacctagaac atcattgtcg
    21721 taattatcgc caacgtataa ggtctcttct ggaagcatat gaaattcttt ctcagctagc
    21781 tgaaaaatct ctttttctgg tttttggaaa cctgttgctt gagaaatgag catattgcgg
    21841 gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc
    21901 ccatttgtga taattcctaa tgggacattc ttttccttca agaaatcgag ggtttttttg
    21961 acctcctcat gcaagcagat attatcaagt tcttcctcgt aaattttttg aaataagagg
    22021 ccatcttctt ccgtaatatg agggtagtct aaatctttta gtgattggct gatccgatgt
    22081 gctcgcatat attctagtgt ccaatctcct gccattactt ttgggaagtt ttcatcacta
    22141 tgatggcgga accggatata tagtggatgc atatctgcat ctgaaacaag cggaacgact
    22201 cttttgaccg cgtttcgaaa tggctgctgc tgatcataca tcgtatcatc aacatcaaac
    22261 acaactgatt taatcaaaat tttacacgcc tttcggtttt cattgatttt ttcacaaatc
    22321 cttgactatt gtaacagaaa aaattgtcta aaaaagctga taaataaagg tttttcttac
    22381 tatatttcta tttccttgac agaataaggt ttttatattt cagttttttt cactaaaaat
    22441 cgtgaaacac gtcaataagt ttgatgaaca aattaatcat tatggtagaa gtcgattgat
    22501 ttattttaat gcaatctctg tggaattttc gaaagattta tttttttaat acagtctcaa
    22561 tataactatt caaacataaa ataacgttat ttgatttatt tgtttatagt aaatttt
//
LOCUS       DADXTG010000036        22450 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.036, whole genome shotgun sequence.
ACCESSION   DADXTG010000036 DADXTG010000000
VERSION     DADXTG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.036"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..615)
                     /locus_tag="IWI57_001163"
     CDS             complement(<1..615)
                     /locus_tag="IWI57_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBC2449322.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLP"
     gene            complement(628..1344)
                     /locus_tag="IWI57_001164"
     CDS             complement(628..1344)
                     /locus_tag="IWI57_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBC2449323.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            1674..2282
                     /locus_tag="IWI57_001165"
     CDS             1674..2282
                     /locus_tag="IWI57_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBC2449324.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            2607..2930
                     /locus_tag="IWI57_001166"
     CDS             2607..2930
                     /locus_tag="IWI57_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBC2449325.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            3112..4482
                     /locus_tag="IWI57_001167"
     CDS             3112..4482
                     /locus_tag="IWI57_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBC2449326.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(4612..5913)
                     /locus_tag="IWI57_001168"
     CDS             complement(4612..5913)
                     /locus_tag="IWI57_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBC2449327.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(6267..8225)
                     /locus_tag="IWI57_001169"
     CDS             complement(6267..8225)
                     /locus_tag="IWI57_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBC2449328.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(8402..8668)
                     /locus_tag="IWI57_001170"
     CDS             complement(8402..8668)
                     /locus_tag="IWI57_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBC2449329.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(9178..11304)
                     /gene="pnp"
                     /locus_tag="IWI57_001171"
     CDS             complement(9178..11304)
                     /gene="pnp"
                     /locus_tag="IWI57_001171"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBC2449330.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(11492..11761)
                     /gene="rpsO"
                     /locus_tag="IWI57_001172"
     CDS             complement(11492..11761)
                     /gene="rpsO"
                     /locus_tag="IWI57_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBC2449331.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            12012..12575
                     /locus_tag="IWI57_001173"
     CDS             12012..12575
                     /locus_tag="IWI57_001173"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBC2449332.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(12828..13349)
                     /locus_tag="IWI57_001174"
     CDS             complement(12828..13349)
                     /locus_tag="IWI57_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBC2449333.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(13352..14194)
                     /locus_tag="IWI57_001175"
     CDS             complement(13352..14194)
                     /locus_tag="IWI57_001175"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBC2449334.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(14187..14912)
                     /locus_tag="IWI57_001176"
     CDS             complement(14187..14912)
                     /locus_tag="IWI57_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBC2449335.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15088..15384
                     /locus_tag="IWI57_001177"
     CDS             15088..15384
                     /locus_tag="IWI57_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449336.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            15952..17544
                     /locus_tag="IWI57_001178"
     CDS             15952..17544
                     /locus_tag="IWI57_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBC2449337.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            17557..18297
                     /locus_tag="IWI57_001179"
     CDS             17557..18297
                     /locus_tag="IWI57_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBC2449338.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            18465..19814
                     /locus_tag="IWI57_001180"
     CDS             18465..19814
                     /locus_tag="IWI57_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2449339.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(19942..20532)
                     /locus_tag="IWI57_001181"
     CDS             complement(19942..20532)
                     /locus_tag="IWI57_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBC2449340.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            20679..22031
                     /gene="brnQ"
                     /locus_tag="IWI57_001182"
     CDS             20679..22031
                     /gene="brnQ"
                     /locus_tag="IWI57_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBC2449341.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6443 a         4510 c         4294 g         7203 t
ORIGIN      
        1 tggtaattta tttctattga accaaatcgt atcgatccct gccatatttc ctcctttgat
       61 atcagaagtc aaagaatcac caatgatcac agtttgcttt ttatcaaaat taggaatgtg
      121 ggaaaagaca tgatcaaaaa attctttcat cggcttttgc gctccgacag cttctgagac
      181 aaagatatcc ttgaagtaat caagcattcc tgcatctgtt aatcgccgat gttgcgtgtg
      241 cgacacaccg ttcgtcacaa tataaagatc agcatgcgga tggacctttt tcaggatttc
      301 tagacttcct ggaacaagat catgaccttg gttcaagtag tggcggtagc gtttttccat
      361 ttgatcacta tctactgttt tcccaaattg ctggaaaagt aacccaaaac gactgcctat
      421 tatttcttct cgtgatagtc tgcctttttc aaactcctgc cataaatgat gattcaaagc
      481 cttatagctg gtttcgattt ctggtgtcaa tgtgattcct tgttctgcaa acaaagcact
      541 taaagcattg gcttcagtta attgaaaatc taacaatgta tcatctacat caaacaataa
      601 agttttatat ttcatctttt tgacctccta tttaaaataa atacttaact ttttcatctc
      661 tgctaatgtc aacgatcgat actcgcctac tcctagctgt tcatctaatt ccaatggtcc
      721 catgcttagc cgtttcaatg cggtcacttt ctttccttgg gctaagaaca tctttttgac
      781 ttgatggaat tttccttctt ggatcgtcaa taaaaccgta ctttggtgct tgtctgcttt
      841 gagtattttt aaatcagccg gcttacattg atacccatca gcgaatacga ttccattttc
      901 aaatgctgtt acgtctgccg gtgtcaccct ttcgttcaca actacttgat aagtctttga
      961 aacctttttt tctggaagta gcatttcata tcccaattgt ccattcgaag tgatcagcaa
     1021 tagtcctgtt gtatctctat ctagtcttcc cactggatat aatggttgga aacgatcggc
     1081 acttgcaatc agatcgatta ctgtcgtctt ttctggatct gatacagctg taacagcttt
     1141 ttcaggttta ttgagaagat aataggactc atttgtttct aagtgttcac ctgctacttc
     1201 gatttgatgc agtaaactat ctacatttcg attttgtcga aattcgactg tgccatctat
     1261 catcacttgc ttcaaagcaa acagccgctt catttccttg cgtgtcgttt ctaatttttc
     1321 ttctattaat ttatctaaac gcatgctgtt tcctttctta ttactactaa ttaattatac
     1381 cttgaatatt cttgcttgca aatacatcat catagttatt ccttgactta attaacagtt
     1441 cacgttacgg ttaaatagta aagtgaattg gatttctcat acacagcgta atgacctggt
     1501 ggagaaaagt tgaatcctga ctttctaagg ggagatgcgc gcatctctcc ttttgcgctt
     1561 ttttaagcgc cctgccttcg atgatgtgca cgagattagc ctttagaatt cacttaaata
     1621 aaatctgtcc gttaccaaag cagacagtca cattttaagg aggaattttg gttatgtcag
     1681 tatcattaga agtaagtaaa agagaagtac gtccccgttc attaagaaac aaacttcgtc
     1741 acgaagggaa agttccagct gttgtttatg ggtataaagt agaaagtacc cctatctcat
     1801 tcgatggttt gacactaagc aaattgttgc gtgaacatgg agcgaacaca gtcatcacga
     1861 tggacatcga tggacaaaaa gtcaatacat tggttcacaa gatccaaacg aacactttta
     1921 ccaatcattt cgaacatgca gaatttctat ctgtaaacat gtcggaagaa acagaagtcg
     1981 aaacagaagt cgttcttgtt ggcgaatcag tgggtgctaa atctggtgga gtgttagctc
     2041 aaaacttgta tacagtagtc gtatctgcta caccagacaa attgcctgaa cgaattgaag
     2101 ttgacgtgac aaacttggaa ttaggtgatt ctattacagt agccgatctt ccaaaaaacg
     2161 acgattacaa attcgtgacc gacagtgaag agcaaatcgc tgctgtagtc gctcaaagtg
     2221 aagaagaaga aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat
     2281 aatactgaaa caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt
     2341 tttatttcta atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac
     2401 gaaaaatcct aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt
     2461 tatactttga aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg
     2521 aaccctcgtt gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat
     2581 tcgttgattc gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt
     2641 acgcttcagt tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta
     2701 ttattgacct agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga
     2761 tcgaaaatcg tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg
     2821 atattgattt gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg
     2881 acggtacgag agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat
     2941 aaacgtggat gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc
     3001 ttggcttctt tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga
     3061 taaacgctat atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat
     3121 ttaacaaaag gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga
     3181 aatgtttttc agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt
     3241 aaaaatgcgc tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt
     3301 gcccaaggtt taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat
     3361 tatcggggtc tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg
     3421 attttaactg tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct
     3481 cctgaaatac ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca
     3541 tcagtcagtt tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca
     3601 ctattctttc tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc
     3661 tattttggca tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc
     3721 agcgtactgt gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa
     3781 gatttcaaat ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg
     3841 tttcaatctt cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta
     3901 gggacagatg tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag
     3961 ccgatgatgt catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa
     4021 gaatatggcc gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt
     4081 ctcttagcag gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc
     4141 ccatcagaaa cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt
     4201 tactggatct tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc
     4261 aagatcccta cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt
     4321 cttacaggaa tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt
     4381 tctgttgccg tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt
     4441 gaagaggaac atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc
     4501 gttgcggcta aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt
     4561 tttttagata acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctgt
     4621 ttttctttcc gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac
     4681 cgtgaaacgg aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct
     4741 agaagcaaag cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg
     4801 aaaataacgc tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga
     4861 ctgttccgct atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc
     4921 ttctaaaata tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat
     4981 agcaatatgc ttaccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc
     5041 agttgttacg atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga
     5101 ttgcttgatc aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat
     5161 aaccggatag cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc
     5221 tgaagcttcg aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc
     5281 atcaaattca aacacgtttt ccaaataata atattcttct ttggtcaact ctcctttttc
     5341 ataagaattc aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga
     5401 tacgagacgc acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat
     5461 aaaaggaaac attactttat aaaaataatg aagtggttta actacgaaca agacgacctt
     5521 ctccgtattg acgatactat agctcttagg aagcagttcg ccaataacca catcgatata
     5581 cgtaactaaa ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg
     5641 tagtttaccg atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat
     5701 gatcaaaccg gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg
     5761 tgtaacatga agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct
     5821 tgatcgtctg attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat
     5881 catcaaaaat aaaataatga aattgataac catattctcc cgcctttctc tttttctatt
     5941 gtaccctttt tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag
     6001 gagatgcggc atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt
     6061 gctaaacagc tttttcatga cctctgataa aaacggtatt caaaagcttg catctacggc
     6121 attaagccca aacagatgaa aaacatgaga agctgttttt atctgctcgt tcttactgct
     6181 tgttgcagta cagctttttc atgacctctg ataaacggtg ttcaagtagc tgacattcga
     6241 cattagttca ggtcaaagcg ctactttcac aaccgctctt tcaaccatgt tttccgagta
     6301 tttccaatga taagcggaac agtttcatgt tttacttcgc tgtactctag cccaaaccat
     6361 gtttctggcg aggtagcgat ttttttgatt gccagttttg cctgcatgat ttgacgttgc
     6421 cattttccta gagttgtcac attggaaact acttcatcaa tcaggacaaa ttggaaatcg
     6481 ccaacttctc gccctggtgt cagagagtat ctttgtggct gttttggcaa acggccttgt
     6541 ttcatcaaat catggatgac ttggcgaaca tatacgttga tttcctgttg gaccctgaaa
     6601 cctaagaaca gattcacttg gacaataaaa tcagtgtcca tcatatctac ttgatattct
     6661 ttcgtatatg gctcatccgt cacttccaca tgaacaaacc aatagactcg tgctctttta
     6721 ggccgtttat ctaaaatcga atagatgaac tgtcggccaa ctttatcttc agccatatca
     6781 ggaaccaaga agaccacatt tgtctgatag attggtaaag aagtatcatc acgtaattct
     6841 ctaagctgcg ggatataatc tctcaaagac acatcctcgg ttgacgattc gcgaataatg
     6901 ttcccttgtt cccaaataaa catcacgact aagatgatca aaccaatcag aacggctaca
     6961 aaaccgcctc ggaagaattt gacaacactt gagataaaga aaacgacttc tatcgcaccg
     7021 aagaaaaagg taactaaata tgcaagccaa cgagaatgcc cattttggat aagataaaag
     7081 taaagcagtg ctgttgtcat caacatcgtc acagtgatcg atagaccgta agcagcttcc
     7141 atatgatgag acgttcggaa tgtgacaacc actagagaac aggcgatcca taaaatcaga
     7201 ttgactgctg ggatatacat ttgcccgata ctgttcccag gatacatgat ctttaaccga
     7261 ggcagcagtt tcaacttgat tgcttctgaa actagcgtaa acgaacctgt aatcaatgct
     7321 tgcgaagcaa tgactgcagc gactgttgca aaagcaacgc cgatcacgat ccaactttga
     7381 ggcatcattt ggaagaatgg attcaggttc ttgatttgct gtgcttcagg gttttgataa
     7441 acattcaaca accacgcagc ctgcccaaaa taattgataa ccaaacaaat tttgacgtaa
     7501 ggccagctca gacgaatatt tcgtttgccg acatgtccaa gatcggaata aagcgcctct
     7561 gcacctgtcg tagccaggaa aatatttcca agaatgaaca agcctaattt gttatctgca
     7621 ctaaaaagca gctggatagc ataataagga ttcagtgcac ggataactgt ccagtcttga
     7681 ctgaagttca ttaatcccat tacgcctaaa aaagtaaacc aagcgaacat gattggtccg
     7741 aatgcttttc cgacaatatc tgtaccaaag cgttggacca tgaataaaac aaaaataata
     7801 actaaagtga taatgacgat cacgttctgg ttattaccga accggtcaaa aaactgcgga
     7861 attcctcgca agccttcaat cgcagtcgtt accgtcacag caggggtcaa taccccatct
     7921 gctagtaagg cagctcctcc gatcattgca ggaatgatca aatatttccc accttttcgg
     7981 acaagtgtgt agagtgagaa aattccacct tcaccatggt tatctgcatt caatgcaatc
     8041 acgacatatt tgattgttgt cagtagagtc aatgtccaaa aaatcaatga gactgatccg
     8101 ataacaaaat caggattgat tcctgacaat ccgccattat cttcaacaat cgatttcata
     8161 acgtaaagcg gacttgtccc gatgtctccg tagacgactc ccatcgctac aagcaggcca
     8221 gccattgata tctttttggt attgtgcgtt gttgccacgt ttttccctca tttcttgtta
     8281 tatggatatc ctttaggtaa gcttgaatga tagatgatat tcttatccat gacacccgct
     8341 ttcctaaatt gcttctaatt atccaagtaa cacactgaag tgtcaactta tttgaataaa
     8401 attattcttt ctttggacgt tctctgatac gtcttgccac aaatgaataa agagcaaggc
     8461 aaaaaattgc acccaataat gctggtatca aataaaaacc gccgagttcc ggtcccagtg
     8521 gtcctagtaa aaattcgccg agccaactac ctaaaaaacc gataatgata tttccagata
     8581 cgcctcccgg gacttccttt cctaaaattg cgccagctat caaacctaat ataccgccga
     8641 taaataacga ccataaaaaa ttcagcatct cctttccctc ctttatccat atttctcact
     8701 tttattttac gctttattca gaaggactcc aaaaatatgc gccaagaaac tccagaaaaa
     8761 aagaagttgt aacaaaagtg ttcagcttat ttccgtagaa gctgcttctg gaacaccgtg
     8821 aatcagaggt tgtgaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
     8881 gttcctcata ttttgccgtt ttttgagcta atatcgaggg acagctgatt gaacatcgtt
     8941 tattggagag gttgtaaaaa agctgttctg catcaagtaa taagaacagc caaataaaaa
     9001 cagctcctaa tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac
     9061 cgtttattcg aaaaaaaagg actgtgacac ggcttttgtc acagtcccct tttttcgaat
     9121 aattattgtc cccctgtcac cttcacgatc acaagtacat tattcctatg aattaaatta
     9181 tttttcttca tttttcgttg aaccatcttc gttcaacatt gcttttcttg acagatttac
     9241 gcgtccttgt ttatcgattt cagttacttt cacaaggatc tcatcaccta gtttaacaac
     9301 atcttcgact ttattgacac gatcgtttgc taattgggaa atatggacaa gtccatcttt
     9361 tcctttgatc aagttgacga atgcaccgaa tttttcgata cggacaactt tccctagata
     9421 aacttcacca actttgactt ctttggtcaa atcttcgatg atcttgatag cttttttgat
     9481 catttctgca tcagctgaag caatacttac ttttccatct tgatcgatat cgattttcac
     9541 accagtttca tcgatgattc cgttgatcgt atcgccgcct ttaccgataa catctttgat
     9601 tttagcaggt tcgatctgga tcatttcgat ttttggtgca tattggctta attcttcacg
     9661 tggagcagca atcgttgaag tcaattcttc taagatttcc atacgtgctt gttttgcttg
     9721 agttaatgct tcagttagga tttgttctgt gattccttga atcttgatat ccatctgtaa
     9781 agcagtgata ccatcttttg taccggcaac tttaaagtcc atatcgccaa gatgatcttc
     9841 cagcccttgg atatcagtta ggatcgtata gttttcacca tcagaaacaa gtcccatcgc
     9901 aataccggcg actggcgctt tgattggtac acctgcatcc atcaatgcta atgtgccagc
     9961 acagatactt gcttgggaag aagaaccatt tgattccaat acttctgcta ctaaacggat
    10021 cgtataagga aattcttctt ctgaagggat tacttgtgct aacgcacgtt cacctaatgc
    10081 gccatgtccg atttcccgac gccctggaga gcctgcacgt cctgtagaac caacagaaaa
    10141 ttgagggaaa ttgtagtggt ggatgaatcg tttgctgact tccaccccta aaccatcaat
    10201 gatttggtgc tctcctagag gtgcgagtgt acaagctgat aaagcttgtg tttgtcctcg
    10261 tgtgaacaaa cctgatccat gtacacgtgg caataagctg acctctgatg acaatggacg
    10321 gatttcatcc aatttacgcc cgtcaggacg gatcttatcg atggtgatca attcacgtac
    10381 gacatctttt tcaagatctt ctgtgatttg tttaacttct ttcaacaagt gatcttcatc
    10441 ttcatgacct gcaaatttct cagcataagc ttcgcggata tcgatcttca cctgctcgat
    10501 attatcttcg cgagcaagtt tttcttcagt cattactgct gctttcattg tagcgtaaga
    10561 agcatcaaag atttcttttt tcaatacttc atcaacttgc agtaaagtca cttccatttt
    10621 ttctttccct actgcttgaa cgatttcttc ttggaaagca actaactctt tgatagcgtc
    10681 aaaaccaaac agcaatgcac caagcatgtc ttcttctgaa acttctttcg cgccgctttc
    10741 aaccatattg atggcttgtt tcgttccggc aacagtcaat tcgatatctg tttgttctgc
    10801 ttgttctact gttggattca agacgtactc gccatttaca cgaccaactt ctacgcctgc
    10861 gattgggccg tcaaatggga tatctgagat tgatagtgct aaagaagaac ctagcattgc
    10921 tgccatagct ggcgagcagt ctgtttctac actcatcacg atgttcgtta cttggacttc
    10981 attacggaag ccttctgcaa acatcgggcg aattggacgg tcgatcaaac gggcagtcaa
    11041 tgttgcttcc gtacttggac ggccttctcg tttgatgaaa cctccaggga ttttccctgc
    11101 tgcatacatt ttttcttcat aattgatcgt taatgggaaa aagtctgtat cttttgcttc
    11161 tttagaggcc acagctgcac ttaagactac tgtatctcca taacggacta aaacggctcc
    11221 gtttgcttgt tttgctagct gaccgacttc gatttgtaag ggacggccgc cccatgtcgt
    11281 tttgaatact tgtttttctg acataacttc tccttttcct tctcgtgaaa gcaagacgct
    11341 actaaacaaa tgaaaactgc cgaaagccgt cttttgagca gctttcattt ggttagtagg
    11401 agtccatcta gcttgatcac ggaattttac ttaattgata tacgagtaaa aaaagtgaga
    11461 ttcatgtgaa tcccactctt tttgactaaa attaacgacg caagcctaaa cgtttgatca
    11521 attcgcggta acgttgaacg tcagttttac gtaagtaagc taacaagtta cggcggtgac
    11581 caactttttt cattaggccg cggtatgaat gatggtcttt tttgtgcacg cgtgcatgtt
    11641 cattcaagtg gttgatttct tcagttaata cagcgatttg tacttctgga gaaccagtat
    11701 ccccttcatg gcgagcgtat tcgttgatga tttcattctt gcgttctttt gaaattgcca
    11761 tttctttcac ctctttcgat tattcccctg cgactgagta aaacgttggt gattcgctta
    11821 accaagtggc aggtcatgtg cctagagcac acagctatac tttacctgaa accgggttga
    11881 aaatcaagta gttaatcatt aagaaaaggt agttttcaaa caatcttact actacttttc
    11941 catttttgtg gtaaactata atgcgaaaat agacttgaaa cacagtcttt taagaaaggg
    12001 gtacatattc catgattaca atggatgata tcattcgtga aggaaatccg accttacgcg
    12061 aagttgcaaa agaagtttct cttccattaa gcgaagaaga tatctcttta ggaaaagaga
    12121 tgcttgagtt cttaaaaaac agccaagatc caatcaaagc tgaagaacta cacttacgag
    12181 gcggtgttgg cttagctgct cctcaattag atatttctaa aagaatcatt gctgttcacg
    12241 taccaagtcc tgacccagag gcagatggac cttctctaag tactgttatg tataatccta
    12301 aaattttgag ccattctgtt caagatgctt gtttagggga aggtgaaggc tgtttatctg
    12361 ttgatcgaga agtaccagga tatgttgttc gccatgccaa aatcactgtg tcttattatg
    12421 atatgaatgg tgaaaaacac aagattcgtt tgaagaatta tgaatcgatc gtcgttcaac
    12481 atgaaatcga ccatatcaac ggtgtgatgt tctatgacca catcaacgat caaaatccat
    12541 ttgcactaaa agaaggcgta ttggtgatcg agtagagaat tggcaggttc cttcgatact
    12601 atgctacaag aaagctttat aaaaaagggg agtgctcaaa agctgcactt ccctttttta
    12661 tgtttctcca ctaatcgaaa tttagataga catctagaaa tcgcaattcc aaaaaattct
    12721 gtgtattgga tacgcatctt ttcttatttt ttcttatttc ttccatatgg gtgtttatct
    12781 cagagatgat agaatagttt ctaggataaa aagtagcagc tacgatgtta tccttcgtag
    12841 aggattcctg cttcagctag tttttgtttg atgtttttca gcatatgctt ttctgggtag
    12901 cggatcgtat gcagatggat gccgcctgtt aattcggata acaaagtagc attacttttt
    12961 tgataacgtc tcataaagga atcgatatct tgtggcgttt tgatgtgcaa agttcctgct
    13021 aactcaccgt aaaggggatg ttccacaatt acatcaatga tttcaccacc attttcaacg
    13081 attagtctca gttcttcttc tgtttgatct tttccatgct ggacagcgat tttactgatg
    13141 tgcccagtat tggtttcttc tgtttcgagg atataacctc ttgctgttgc cacgatactt
    13201 ttacctgcag ctcgtagtaa agcaatatcg cctacaatga tctgacggct gactccaaaa
    13261 gttttcgcaa acttgctagc actcactggt ttttctgtat ttgccaaggt ttgcatgatt
    13321 tgttttcgcc gtgtctctcc ttcgatcaca cttattcctc cttcattttg ttttggtatt
    13381 cgcgcgagtg tttcatcaaa tcaacatacg tgtctgttat tgtttcctta tatcccttat
    13441 ttttcagcag actaacaact ttatccaata cttgctgctc tctttttgca tcataaactg
    13501 cttggttttc ttcttttttc acttgtccta ttttagttac cgtatccatt cttttttcta
    13561 ataaagcaac caactctcga tcgatcatat cgatcttatg acgtaatgtt tcaagagact
    13621 cttctgttag ttcttcctct actgtcttga tgatacgagc aggatttcct gcaatcacta
    13681 cattgtcggg aaaagatttg gtcacaaccg aacctgcgcc gactactacg ttgtccccta
    13741 gagtgacacc cggcgtgacg ataactcctc cgcctaacca aacattatta ccgattttaa
    13801 tgggttttgc gtactccaga ccgctgttac gtttcactgg atgtagtgga tgcgtcgccg
    13861 tgtaaagctg gacattcgga gcgaacatgc agttatcacc gatttcaatc gtgctgacat
    13921 ctaaaaatgt acaattgaaa tttgcataaa aattctcccc aacaaatata ttatatccgt
    13981 aatcaaaatt gataactggc tccatataga tttttttccc tgtacggcca aaagtctctt
    14041 tcaacaattg ggatcgcagt tccgctgatt ctgcttggtt gatgatttgg ctttgacttc
    14101 ttgcaaacat acggtcggca accagctcag gatcattggc aaaataaagc tcgcctgcaa
    14161 tcattttttc tttttcggtt ttgaattcag tcataatctg ctcctaattc ttgtattttt
    14221 ttgaaaacaa ccgctccgtc ttcttgaata aacgtataag gctccgctaa ataaacaggc
    14281 gtttgattga ttactaagat tttggctgac ggctggcgaa actgtaccag atcacaaaac
    14341 ggatgaactt gaaaactcgt tccaacaatg acgatcagct ctgcttggct gacggcataa
    14401 gccgcctgct ccatgactcc atcttgaaac ccttcaccat ataacacgat gtctgggcgt
    14461 atttgtcctc cgcatgcttg atgtttgtcg ctgtgaagat attctttcca gtctaccgtt
    14521 tgttgacatt ttcgacaatg acattgatac agactcccat gaaaattgac gagttgcggg
    14581 cttttagcct ttgcgtgcaa accgtcgatg ttttgggaaa caacccaaac atttttttct
    14641 ttcgctagat gtgctatttc caaatgaatg atattgggtt ttgcttcagg atgataaatc
    14701 tttttgacaa attgatagaa tttatccggt tcattcacca ttgcttgatg actcagcaag
    14761 tattccggct gctccatccc gtggtagatt cctgacaaag atcgatagtc tggcactcca
    14821 gaaggtgtgg atacccctgc accggtcaaa aatgtaattt gctgataagc taatatctct
    14881 tttgctgctt cgtctactgt taattgtttc atgttttgtc acctcacttt ccattatagc
    14941 ccatactcga acaaatttat tgttgaaaag tcagaaaaat gggaataaaa aagtgttatg
    15001 ggcatttgag caatttatca caatgttcac tgacaaaaga atgaaaacgt gttacaatac
    15061 tgctgtataa aagtgaggtg aatcgatatg ttaccattct ttatttattg gggcgttatt
    15121 ctggcttgta tcgcctggtt agcgttaagc gtttatttct ctgtttttta tcttgtacgc
    15181 aaagaaaatg gaaatctttg ggcttttgct ttattcaacg tgattgccgc aattgtttta
    15241 gccattacat tagccgtata tcgaacatgg ggctggggaa tcacccaata ctcaagtttg
    15301 atttatctaa tattagccat ttatggcgtc gttgttcttt tgcaagcgat cctaggacgt
    15361 gagccaaaga aagcggcagc ttaaatgatc gagatggaac tggacacagg aaattttctg
    15421 ttctagttct ttttttattg tattacatac atttttgtgt ataattatac tatcgtttgc
    15481 aatggataga tgattcacat tttgattgtt ttttcggtaa aagtaaagat ttctatattt
    15541 atcagtattt tttcaaaaac cagcagttat agattgacaa agcctctcga gtatcgtaaa
    15601 ctttttttgt ataaataact gaatacgaga tgcctcaatc aatgaggtag aggtcgcgag
    15661 aatcattaac cctgtggagt gaagcaaaca tggagaagca ggaataggga ggatcgccga
    15721 aatgtgcact tttttgcttg gagtgtatgt tgggacgcaa gttaagagct tgcggactgt
    15781 cttagcagtg atgctaagtt gcgctatgtt ttgtgagagg tttatcacct tatggaattt
    15841 tagcggcctt tttatatcta taaatcggtc gctttttatt ttttcagata aagctttacc
    15901 gcatttcatc tagaccgctc gtgtatcata taatcagaaa gggagaaaaa aatgaaaaaa
    15961 aatatggttt cagttgccgt actatttgct tttgtcttct cactattcag tgtgggatct
    16021 ctggcaaatg cagatgaaaa aacaccgagc gatgagtttc gcgtaggtat ggaggctggg
    16081 tatgcaccct tcaactggtc tcaacaaaca gatgagaata atgccttgcc aattcaaggc
    16141 caaaatagtt acgccggcgg ttatgatgtt caaatcgcta aaaaagtagc agatggttta
    16201 gggaaaaaat tagtcatcgt ccaaacgaaa tgggatggtc tggctcctgc ccttcaatca
    16261 ggcaagatcg acgcgatcat cgccggaatg agccctactg ctgaacgacg aaaagaaatt
    16321 gattttaccg atccttatta cgaatcacaa ttagtagtag tagttcaaaa agatagcaaa
    16381 tatgcaaaag caaaaagttt agcagatctt tctggagcaa agatcactgc tcagttaaat
    16441 acattccact atactgtgat cgatcagata ccagatgtgc agaaacaaca agcaatggat
    16501 aatttctcag caatgagaac agctttagct tctggaatga tcgatggtta tgtcagcgaa
    16561 cgtccggaag gtgtaacggc aactagtgtg aacaaagact tggaaatgct tgaattcagc
    16621 ggtgcagatg gcttccagac aaacccggaa gatgttcaga tttctgttgg tatgcgtaaa
    16681 ggcgacccgg atatccaaaa agtaaaccag attctttctg gcatctctaa cgatgaacga
    16741 accaaaatta tggaccaagc agtcaaagat cagcctgctg caactgatag tgaagaaaaa
    16801 cctggcatca tttctgattt cactacgatt tggaaacaat acggcaatat gttcttacga
    16861 ggtgctggac ttaccttgtt catcgcattg atcgggacag ttgtcggtac tacacttgga
    16921 ttactgattg gtgtcttccg ttcggtacct gaatcaggaa atgcactgac tcgcttcttc
    16981 caaaaaatcg gaaatgcact tctttctatc tatgtcgaag tattccgcgg aacaccgatg
    17041 atggtacaag caatggtcat cttctacggt ctggcactcg cttttggcat ttcattgaac
    17101 cgaacgattg ccgctttgtt tattgtctct ctcaatactg gagcttacat gacagagatc
    17161 gttcgtgggg ggatttttgc agtagatgaa gggcagttcg aagctgcgca agccattggt
    17221 atgacccatg gccaaacgat gagaaaagtt gttattccac aagttttacg gaatatcttg
    17281 ccagcaacag gaaatgagtt tgttatcaat atcaaagata cagcagtttt aagtgtaatc
    17341 ggcgtagctg atttgttctt ccaaggaaac tctgcttccg gtgcgaattt ccaattcttc
    17401 caaacattta cgatcgtcgg gatcatctac ttagtcatga cctttacgat caccagaatc
    17461 ctgcgggcgg tagaaaagaa aatcgatggt ccttcagctt ataccaagct tgaagaaatc
    17521 gacaattcaa atttgcagga ataaggagga agaatcatga atgaaaaaat tattgaaatc
    17581 aaacatctaa gaaaaagctt cggtgaaaat gaagtattga aagatatcag cgtaacagtc
    17641 aataaaggtg aagttgtgac gatcatcggt tcttccggtt ctggtaaatc tactttcttg
    17701 cgctgtatca atttactgga aaaaccaaca ggcggcgaga tcatttataa tggagaaaat
    17761 gtattagctc ctaaatataa tttacctaaa tatcgaacga atctaggtat ggttttccaa
    17821 tccttcaacc tgttcaacaa tatgaatgtc ttggaaaact gtatgtctgg tcaaacaact
    17881 gtcctgaaac gagataaaga aactgccaaa aaagtagcaa tcgaaaatct ggaaaaagtt
    17941 ggtatggatc gttatatcga agccaaacca gcacaattat ctggtggaca gaaacagcgt
    18001 gtcgcaattg ctcgggcatt atcaatgaat ccagatgtat tgctattcga cgaacctact
    18061 tcggcgttag accctgaaat ggttggcgaa gtgcttaata cgatcaaaga cttagcacat
    18121 actggcttga caatgcttat cgtgacgcat gagatggaat ttgcaaaaga tgtatctgac
    18181 cgtgtcattt ttatggataa aggtgtgatt gcagaagaag gtacaccaga agatatcttt
    18241 gtccatccga aagaagaacg tacaaaagaa ttcctttcac gtattttaaa tgcttaggat
    18301 caatagctga ttgggacaaa gagctgcggt gtttaccgca gttctttttt atgaattact
    18361 cataaaaggt aaatttctga aaattttgga atcttagttg tgtttttcag aaaactctct
    18421 taatatggaa gtaattaaac ttttttagtt aggatgattt aaaaatgaat gaagagcaag
    18481 aactgcagcg tggactgaaa aatagacatg tccaactgat ttctattggc ggagcgattg
    18541 gaacggggtt gtttttaggt tccggaaagt ctatccagct agctggccct tcgatattgc
    18601 tcgcctatct tattactgga tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc
    18661 tgttatcaaa caccaataac cattcttttc tggattttgt tgcagaatat cttggcaaaa
    18721 aagcggcttt catcactggc tggacgtatt ggttctgctg ggtatcgatt gcaatggccg
    18781 atttaacagc catcggaatg tatgtacgct actgggcgcc tggtgttcct cagtggcttc
    18841 ctgaactgat tgcattaggt cttcttcttt gcttgaatat ggtggctgtc tcattgtttg
    18901 gtgagttaga gttttggttt gcattgatca aagttgtagc gatcattgcc tttatcctcg
    18961 taggagctta tatgatcctc acccactata cgacagatat tggcacagcc agtatcacga
    19021 atctatggtc tcatggcgga ttctttccaa ctggtgcaaa aggatttatc ttagctttcc
    19081 aaatggtaac ctttgctttt gctgggatag aattagttgg attagtcgct ggagaaacag
    19141 aaaatcctga aaaagtgttg ccggaagcta tcaataatat tcctatcagg atcatcttat
    19201 tttacttagg atctcttttt gtcattatgg caatctatcc atggaacagc ttgaatccag
    19261 ataacagtcc atttgtagaa gtattttcgg aaattgggat cacgattgct gcttccttga
    19321 tcaatttagt cgtattaagt gcagcagctt ctgcctgcaa cagtgcaata tatagtacag
    19381 gacgtatgct tcgttcttta gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa
    19441 caacacatca tgtgccaggg aatgctttga cattttctac tatcgtcatt ttcatttctg
    19501 ttatcttgaa ttacgtgatg cctagtgaag tattcacact agtaagcagt attgccacta
    19561 cttgtttctt gtttatctgg agtatgcttg tctacacgca catgaaatat cgtaaaagta
    19621 tcctcggcaa aaaagctcat tcatttaaaa tgccgttgta cccatttagt aattatctgg
    19681 tatttcttta catggccttt gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg
    19741 ctttattact aacacctgtg tggttcttgc ttctattact aatctatcat atgaaatatg
    19801 aacgtaagaa gtaagaaagt agttgtgaca gaagtggtca gcttcgagaa ataagaagga
    19861 atttacgaaa attgcttctt aaatttttgt gaaatttcgg cttatttctt ggagataaaa
    19921 gcttctgtcc cagcctcttt tctatttccc cggaaacaac cgctgataat aggcatctgc
    19981 ttttttatag gaaaaaatcc aaataccaga tacaaaaaaa cgtccgaata acccgatcaa
    20041 cggtacaaaa aagacaccgg caatcaagca taagttaatc atcatggaga taagacttcg
    20101 acggtccttt gacaaaactc tcacgacatg ggaatctggt ttatttgctt tcatgatttg
    20161 cctcatcaag ataccaaaag aaaaactcca catcgtgtat actaagacat aagccatctc
    20221 aggccaaaaa gcttcagggt tacgcccgac ccaactagta acagaaggac ataaagtcag
    20281 ccaaaagagc aaaaagatat tcgaccagac cactcggtaa ttaattcgtt ctgccatctg
    20341 aaatatacga tgatggttca cccagatcac cgcaacaaat ataaagctga tggtataagc
    20401 taataatgta tttcggatga cccataaaga atgaaagcta gatgtattag ggatcttgat
    20461 ctctaataca agaactctca aaacaatggc taccactcca tcagtaaatg cttctaatct
    20521 cgactttgtc acgatgattc ctcctcagat gattcagcgc ttttatttta tcacttacgt
    20581 tattaaataa ccatcgtttt atatagggaa ttttctgaaa attgttgcat atttttcgaa
    20641 attttgttac ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta
    20701 aagactatct ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta
    20761 tctttcccgt tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt
    20821 tcttggttac cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa
    20881 gctcaggtgt atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca
    20941 tcttgcttta cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt
    21001 tcgagattgg tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt
    21061 tcagtattgt attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag
    21121 attatgttgg aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc
    21181 ttgctttttt gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag
    21241 agcatgcttt tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt
    21301 tagcatttgg gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg
    21361 aaatcgctaa agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt
    21421 atactttgct cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg
    21481 gcgggatcac gctggctcag attgctgatc attatttagg tacttatggc agtatcttat
    21541 tagcatttat cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg
    21601 cagaaacatt cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta
    21661 gtatccttcc ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc
    21721 ctgttttgat gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc
    21781 ctttgtttaa gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt
    21841 cgatttttga tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa
    21901 atcttttata tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg
    21961 taccagcagt tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag
    22021 tagcagattg ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat
    22081 tttgaaaaat ggcttctcac atttttgcat aaatttaact taataccgag aaactgactt
    22141 ttgaacaccg tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata
    22201 ttttgtgaaa atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc
    22261 ttttgaacac cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg
    22321 caaagtagct ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc
    22381 aaacaacatt tcttttataa tatagatttt agacatatat atgatataaa aatagttttc
    22441 gcaaaaaaac
//
LOCUS       DADXTG010000037        22349 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.037, whole genome shotgun sequence.
ACCESSION   DADXTG010000037 DADXTG010000000
VERSION     DADXTG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.037"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..937
                     /gene="purR"
                     /locus_tag="IWI57_001183"
     CDS             119..937
                     /gene="purR"
                     /locus_tag="IWI57_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBC2449342.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1440..2813
                     /gene="glmU"
                     /locus_tag="IWI57_001184"
     CDS             1440..2813
                     /gene="glmU"
                     /locus_tag="IWI57_001184"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBC2449343.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2907..3878
                     /locus_tag="IWI57_001185"
     CDS             2907..3878
                     /locus_tag="IWI57_001185"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBC2449344.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3884..3986
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4052..4621
                     /gene="thiT"
                     /locus_tag="IWI57_001186"
     CDS             4052..4621
                     /gene="thiT"
                     /locus_tag="IWI57_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBC2449345.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4702..5475)
                     /locus_tag="IWI57_001187"
     CDS             complement(4702..5475)
                     /locus_tag="IWI57_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449346.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5742..7442
                     /locus_tag="IWI57_001188"
     CDS             5742..7442
                     /locus_tag="IWI57_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBC2449347.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7412..7630
                     /locus_tag="IWI57_001189"
     CDS             7412..7630
                     /locus_tag="IWI57_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBC2449348.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7650..8540
                     /locus_tag="IWI57_001190"
     CDS             7650..8540
                     /locus_tag="IWI57_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBC2449349.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8558..8842
                     /locus_tag="IWI57_001191"
     CDS             8558..8842
                     /locus_tag="IWI57_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449350.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9329..9943
                     /gene="gmk"
                     /locus_tag="IWI57_001192"
     CDS             9329..9943
                     /gene="gmk"
                     /locus_tag="IWI57_001192"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBC2449351.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9948..10253
                     /locus_tag="IWI57_001193"
     CDS             9948..10253
                     /locus_tag="IWI57_001193"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBC2449352.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10506..12914
                     /gene="priA"
                     /locus_tag="IWI57_001194"
     CDS             10506..12914
                     /gene="priA"
                     /locus_tag="IWI57_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBC2449353.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12929..13420
                     /gene="def"
                     /locus_tag="IWI57_001195"
     CDS             12929..13420
                     /gene="def"
                     /locus_tag="IWI57_001195"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBC2449354.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13413..14351
                     /locus_tag="IWI57_001196"
     CDS             13413..14351
                     /locus_tag="IWI57_001196"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBC2449355.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14351..15709
                     /gene="rsmB"
                     /locus_tag="IWI57_001197"
     CDS             14351..15709
                     /gene="rsmB"
                     /locus_tag="IWI57_001197"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBC2449356.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15722..16462
                     /locus_tag="IWI57_001198"
     CDS             15722..16462
                     /locus_tag="IWI57_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBC2449357.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16459..18528
                     /gene="pknB"
                     /locus_tag="IWI57_001199"
     CDS             16459..18528
                     /gene="pknB"
                     /locus_tag="IWI57_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBC2449358.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18693..19592
                     /gene="rsgA"
                     /locus_tag="IWI57_001200"
     CDS             18693..19592
                     /gene="rsgA"
                     /locus_tag="IWI57_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBC2449359.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19605..20255
                     /locus_tag="IWI57_001201"
     CDS             19605..20255
                     /locus_tag="IWI57_001201"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBC2449360.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20256..20894
                     /locus_tag="IWI57_001202"
     CDS             20256..20894
                     /locus_tag="IWI57_001202"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBC2449361.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            21049..21903
                     /gene="mreC"
                     /locus_tag="IWI57_001203"
     CDS             21049..21903
                     /gene="mreC"
                     /locus_tag="IWI57_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBC2449362.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21907..>22349
                     /gene="mreD"
                     /locus_tag="IWI57_001204"
     CDS             21907..>22349
                     /gene="mreD"
                     /locus_tag="IWI57_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBC2449363.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTL"
BASE COUNT         7451 a         4009 c         4843 g         6046 t
ORIGIN      
        1 tctttttatt ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt
       61 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
      121 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
      181 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
      241 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
      301 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
      361 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
      421 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
      481 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
      541 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
      601 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
      661 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
      721 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
      781 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
      841 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
      901 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
      961 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
     1021 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
     1081 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
     1141 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
     1201 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
     1261 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
     1321 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
     1381 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
     1441 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
     1501 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
     1561 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
     1621 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
     1681 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
     1741 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
     1801 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
     1861 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
     1921 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
     1981 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
     2041 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
     2101 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
     2161 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
     2221 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
     2281 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
     2341 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
     2401 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
     2461 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
     2521 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
     2581 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
     2641 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
     2701 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
     2761 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
     2821 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
     2881 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
     2941 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
     3001 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
     3061 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
     3121 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
     3181 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
     3241 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
     3301 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
     3361 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
     3421 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
     3481 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
     3541 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
     3601 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
     3661 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
     3721 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
     3781 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
     3841 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
     3901 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
     3961 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
     4021 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
     4081 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
     4141 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
     4201 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
     4261 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
     4321 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
     4381 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
     4441 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc cttggctctt
     4501 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
     4561 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
     4621 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
     4681 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
     4741 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
     4801 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
     4861 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
     4921 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
     4981 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
     5041 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
     5101 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
     5161 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
     5221 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
     5281 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
     5341 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
     5401 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
     5461 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
     5521 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
     5581 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt tatttaaagg
     5641 aggttttatg aattgttttc ttatggaatc tacaaagtcg aatacgagac tcatgacctt
     5701 tttatgacgt actggtatac ttgaagaaaa aggagagcgc gatggagata aaaaaagttt
     5761 acttttcaca gaaaatgtcg ctgtattcgc ctatccagca agcaaaacaa ccccttccat
     5821 tttttcaaag tcatcaagaa agtagagacg ctttttggcg ttctgcaaaa aagcaatgtg
     5881 cttttaaagc agtatcgaaa aaatataccg tctattttac tattgctaaa agtagaggag
     5941 atgttatttt taataatctg ttgattgaag gtgaaacttt tgaatggaaa aaattttttc
     6001 ggtatatgga agcttgcgct cgttttttta ttaaagaaaa ctgttgtttt ttatttcaat
     6061 ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa cggctatcaa ggaaccacac
     6121 aattaaataa aaagctggtc tatcatacag cacttgtttt aggtggagga ggagcgcacg
     6181 gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca tgatatcaac tttgagatta
     6241 tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat tttacaagga gatatgaaaa
     6301 aagctgtgaa tttatggaaa aagctgtcga caagacaagt gcttgcgtta ccagaaatgg
     6361 ctgcagttga agaccttcgt gaacgcttct atcgagaaag acgccaaatg acgaaaacag
     6421 cactaattga aggcggggtt tcttctgctc cattggaaaa gatggtcaaa gacaatttgg
     6481 atttgtcctt actcaaacat aatcccaaaa tacgtttata tacggtgtct actaaactgc
     6541 cagaactagc ggaagtagtg actgatatcc aacagacaaa aacagaagaa ataccagatt
     6601 ggatactcgc ctctgcttca ttttatcccg ctatggctta tcgaaagatc ggaaaaaata
     6661 aatatgcaga cggtgggtat cgcaacaaaa tacctattga tattgcgatt aacaaaggag
     6721 caacagaggc atttgtcgta gatgtgcaag ggccaggccc agcgaaaaaa attcgtatgc
     6781 cagacatttt tatccattgg aaatgtcaaa cattgtggac actaggcagc tttctgcttt
     6841 ttgatagcca aaggaaccaa ttaaatttac aattaggcta tctggaaatg aaaaaacgat
     6901 taggctatta ctatgggaac tggtatacat ttcattctgt taaacctgca agaacatact
     6961 ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt agaattatct tttttcaaaa
     7021 gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa cagggtcgta ccagagacat
     7081 gcggattggc gatgctagaa cttttagcaa aaaaacattt tttactgccc aatgaaatct
     7141 atcaagtaga catgatgatt caaatgatct gccaacaaga tataaaaagt atgccggatg
     7201 atttattggc acaaattggt caattaagta ctgaagaatg gcgaaggtat cgaaaatacc
     7261 agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt tgattatctg ctacaaggaa
     7321 aacgcaagga tcgtttgcag catgaattgc ttaagcaacc agttgacaca ttactgatcc
     7381 tatatttata ttatctcaag gaggaacaac catggcacaa gaattttcat atgaaatcgt
     7441 agaagaaatc gctatattgt cagaaaacaa caaggggtgg cgtaaagaac tgaacctagt
     7501 cagctggaat ggtcgcccgc caaaattcga tcttcgagat tgggcgccag accatgaaaa
     7561 aatggggaaa ggcctgacat tgacgaacga agagtttgaa caactgcaaa aagcaataga
     7621 aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat gactggtttt ggcaaagcca
     7681 tccgcgaaac aaaagcgtat cagttagata ttgaaataaa aagtgtcaac cagcgttttc
     7741 tagatgttca aatacgaagt cctaaaatcc tgaactttgt tgaaaacgat tttcgtcagc
     7801 ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt tgtgaatcta gcttatattg
     7861 gcaaaaacga aaaacagatc gtcatcaact gggaattgat tgattcactt atcactcaat
     7921 tgacagacgg aattcaacaa cgatacggta gtcaaaatca agtgtcaatt agtcggatgg
     7981 cggaacgctt cgtgctggac gaaaacttcg taacggtaga agaaaaaccg gaagagagca
     8041 tggaagaatt ggcacagtta gctctagata cattgaaaag cgcacttgat tctattgagg
     8101 cgagtcgaga aaaagaaggt caagctttag ctttagtgtt gaaaaagaat actgaagaat
     8161 tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat ctatgaaaag gaatttcaaa
     8221 taaggtatca aaaaaagctg gaggaatttc ttggagcgaa agtcgatgag caacggttgt
     8281 tgacggagct agccatcttg ttggaacgcg gagatattca cgaggaactg gatcgtctgg
     8341 cgatccatgt acaaaaacta gatgaactta tcgaagcaca atgtcctgta ggtcgggaat
     8401 tagatttcct gatccaagaa atgaatcggg aaatcaacac gatcggttcc aaatcaaatg
     8461 caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat cttagaaaaa atcagagaac
     8521 aagtacaaaa tattgaatga cgaaagaggt agagataatg aaatattatg acgtaacgtt
     8581 ccacgaatta agtggaaaaa ctgtggttaa acgggatatc ccttctgaaa aaaacggctt
     8641 tgacgtatgg aaagatgcct gtgcagacta taatgagaac gaactgttta ttttgatcaa
     8701 cgacggtgct tacgtgacga tgaatcggaa atttattgtt cgaattgaca cagaagaggt
     8761 agaagatccg acagaaaaag cacgttctcg aaaagatgag atcatgggtg tagtgaatac
     8821 tttgtccaat atgggctttt agcaaggatg ctgaacaaag aaatacaata aataaacaga
     8881 attggatttc ttcgcttagc ttctttgctc taggaataca tttaaaggtt gtgacaaaat
     8941 tcgtgtcaca atcttttttt attttgaata agtgatgttc aaaaaccatt ttctcggtat
     9001 taagccaaat ttatgcaaaa atgtgagaaa ctattttcac aaaatttttc ttcatactcg
     9061 aaactaatcg gcttcttcac gacctcttat acacggcgtt caaaagcttg catctgcgat
     9121 aataacccca accaaacgaa aaacatgaga agctgtttat gtttggctgt tcttattact
     9181 tgatgcagga cagctttttt acgacctctt ggtacagggt agctttttta ccaatttcta
     9241 gctataatta aagataacct tgaaagttgc agaaaaaaag tgcaaaataa acgataagta
     9301 caaaaagact agttgaaagg acgtcgtcat gtcagagcgt ggattattaa tcgtgttgtc
     9361 tggtccatcc ggtgtaggaa aaggcacagt acgaaaagca atttttgaga gtgaggataa
     9421 tgattttcag tattcgatct caatgactac ccgtaaaatg cgtgaagggg aggtcgaagg
     9481 cgttgattat ttcttccgaa gcaaagaaga gtttgaatca atgatcgaag ctggagagat
     9541 gctggaatac gcagaatatg tcgggaatta ttatggaact cctttgtcat atgtcaacaa
     9601 gacattggat gaaggaaaag atgttttctt ggagattgaa gtccaaggag caaaacaagt
     9661 caaagaaaaa gtgcccgatg gtgtattcat ctttttgact ccgccagatt tagcggaatt
     9721 aaaatcacgg atcgtaggcc gaggaacaga tgcagatgaa gtaatcgaaa aacgaatgaa
     9781 agttgcaaaa gaagaaatcg aaatgatggc tctttacgac tatgctgttg taaatgatga
     9841 agtgccgcta gcagttgaac gaatcaaaaa cattattgcc agtgaacatt ttcgagtaga
     9901 tcgcgtaatc ggtaaatata ttaaaatgtt aaaggagatg tagtcttatg atgttaaaac
     9961 cttcaattga ctcactatta gatcgtgtaa attcaaaata ttctctagtg attttagcaa
    10021 gtaaacgcgc gcatgaattg gatgcaagtg cacagcctac tctagattct tttgattcag
    10081 taaaaagcgt cggtcaagct ttagaagaaa tcgatgcagg aaatgtagtc aatgatccac
    10141 atcctgaatt aaaacgtgaa cgtttgaaga tggaagaaga ggaacgtcaa gcacaaaaag
    10201 agcgtgaaca acacgaattg gaatcacgta tccgtgaaga gcaaaaaatg taataaagaa
    10261 aaatagagcg aacatctaaa aagaaaagca aacccagctg tttccttttc aaacattcgc
    10321 cacttattgt ctggtagaaa aggctgtgtc tgaacttttt gacacagttc ttttttttct
    10381 tatctgctgt taattttttg ttaggatttg gcagaagata agacaaatcc atcgatctct
    10441 agtaaaatag gcaaggaact ttttgtcgct tgataagaaa agacaaaaaa aggaggaaac
    10501 gaaccatgac attaacagct gaagtcattg tggacgtacc tactatgcag accgatcagc
    10561 cattcactta tttggttccc tctgaaatgg aaacagcttt gcaagtcggg atgcgagtag
    10621 aagtcccttt tggtaatggc aatcgtcatg tccaaggatt tgttatggct atccaaaaat
    10681 cagaagaatc agctaatcca tctttgaagg cagttattcg tttgctagac cttgctccgg
    10741 tggtcaatga agaattactt tcattagctg attatatgaa aaaaattact tacgcattca
    10801 aaatcacttg tctacaaacg atgcttccga gtgtgatgaa agcagaatat gataaattga
    10861 tctatccttt ggcagacaca ccagaagttc aggtattgtt tgatcagcga gaagtgattt
    10921 cctggaagga agcagaagaa gaaggcagcc tttctcaatt gattcgctgg cgccaagagc
    10981 aattagtaga tatcaaatat gaagtccata cacgaaataa agtaaaaacg attcgtttgg
    11041 tccgttcttt gttaactgaa aagcaaatcg aagaagaatg ggcaaaatta cggcaaaatg
    11101 ccaagaaaca aaaagaactt cttttgtgtt tatctgaact gagccaagaa gaaccaattg
    11161 cttatttcaa aaataaagga atcagtactg ctgtcttgaa tcaaggaaaa gaaaagggct
    11221 ggctggagtt tatagaatca gaacgttatc gcgaccctta taaagaccga gtatttgacc
    11281 aaacgacggc attggaactg aatgcagaac aaaaaaatgc tgtcgaacaa atcatcacag
    11341 caggtcagca gcaaaaagat caggtctttt tgctggaggg aatcactggc agcgggaaaa
    11401 cagaagtata tcttcaggca atcgctgatg ttttgtcaga aaataaaacg gctattatgt
    11461 tagtgcctga aatcgcattg actccgcaaa tggtcgagcg attcaaagga cgcttcggag
    11521 aatcagttgc ggtcttacat agcggacttt cccaaggtga aaaatatgat gaatggcgca
    11581 agattgagcg ggaagaagcg caagtagtag taggcgcacg atctgctatt tttgccccgc
    11641 tgaaaaatat cgggctgatc attatcgatg aagaacatga atcaagttac aaacaagatg
    11701 agacaccgcg ttatcatgcg agagatctag caatttggcg cagtaaatat catcattgcc
    11761 caatagttct aggaagcgcg acgccctctt tagaatcaag agcacgagct caaaaaggcg
    11821 tttatcagtt attgcagtta aaccaccgag ccaaagcagc tgcacagctt ccagctattg
    11881 agatcgtaga tatgcgtgaa gagtttcaga atcatcggac ctctactttt tctgctaatt
    11941 tgcaggaaaa aatacaaaat cgattagaca aaaaagaaca aactgtccta ctgctcaatc
    12001 gtcgaggata ttcgtctttt gtgatgtgta gggactgtgg atttgttctt ccatgtccta
    12061 attgcgatat ctctcttaca ctgcatatgg atacacgttc aatgcgctgc cattattgcg
    12121 gacatgaaga acctattccc aatcgctgtc ctaattgtgg cggcaataaa atccgctact
    12181 atggaacagg tactcaaaaa gtagaagagg aactccgaga actttatccg caagctagga
    12241 ttttgcgtat ggatgtagat accactcgaa gaaaaggggc acacgaacag attttacaaa
    12301 aattcggtgc aaaagaagca gatatattat tagggacaca aatgattgct aaaggtttgg
    12361 actttccaga agtgacatta gttggggtgt tgaatgctga tacctccctg aatttaccgg
    12421 attttcgttc tagtgagcat acattccaat tgcttaccca agtttcagga cgagcagggc
    12481 gagcggaaaa agcaggcgaa gtcgtgatcc aaacattcaa tcctcaacat tacgccatag
    12541 aattggctaa aaaacaaaat tatgagcagt tttatcagca ggaaatgcat gtacggcatc
    12601 gtggaggcta tcctccttat tactttactg tgaaaataac cgccagccat cctgaagaac
    12661 aagtagcggc aaagaaaatc tttcagattg ccaatcagct aaaagaagtg ttaagccctc
    12721 aaagcttgct attagggcca acgcccagca tgattttgag agtgaaaaac cgatattatt
    12781 atcaactaat catcaaatat aaacatgaac cgaatcttcc tcatgttttg gatgaaattt
    12841 tgaatggaag ccagaaagaa cagcgacaag gtctttttgt ggcaattgac aacgaaccat
    12901 taaactttat ttaagaaagg gccaaattat gcgctatcct atattgatcc accctaatga
    12961 taaattaaaa agaactgctc agccaatcga tgtaatcacg gacgagacaa tcgctttatt
    13021 agacaattta tatgagacaa tgattgcgaa tgacggaata gggatcgctg ctcctcaagt
    13081 aggacaaaac aagagaattg ctgtgatcga agtagatgaa ggcgagaaat ttgaactgat
    13141 taacccagaa atcatcgaag caaaaggtga aagccttgac gtagaaggtt gtctcagtat
    13201 tccgcatgtc tatggaacgg tcaagcgagc agatgaagtg accgtgcgtt attatgatcg
    13261 agacggagaa gagatagaag tgacggcttt tggctattta gcccgtgcct tccagcatga
    13321 aatcgatcac ttagacggta tcttgttcat tgagaaaatg atacagcaga tcccggaaga
    13381 agaacttgaa gaatatatgg aggaacatct tgatgactaa gctagtattt atgggcacac
    13441 ctgctttttc tgtgccgatt cttgaagggc tgatagaatc aggttacgag atccaggcag
    13501 tggtaacaca gcccgaccgt ccagttgggc ggaaacgagt aattacgccg acaccggtaa
    13561 aaaaagcggc tctgaagcac ggtatccgtg tgctgcagcc agagaaaatc tccggttcgc
    13621 cagaaatgga agaaatcatt gaattggtgc ctgatgtgat cgtcactgcg gcatttggcc
    13681 aattcttgcc tgaaaagtta ttgcaagtac caaaattagg ggcaatcaat gtccatgctt
    13741 ctttacttcc taaatatcgg ggaggtgcgc ctgttcatta tgccatcatg aatggggaga
    13801 aagaaactgg tgtaacgatc atggaaatga tcaaaaaaat ggatgcaggc ggaatctatg
    13861 cgcaagaaag cataccaatc actaaacaag atgatgtagg gacgatgttt gaaaagctta
    13921 gccttttagg aagaaagcta ttgctagaaa cattgcccaa tattttggat ggacaaaaac
    13981 cagtacctca agacgagtca gaagcaactt tttcgccaaa catcacacgg gaacaagaag
    14041 caatcgattg gcataaaact gcagaagaaa ttgacaacca agtacgcgga atgcgtcctt
    14101 ggccaattgc attcacgacc tatgagggca tacgctggaa attattatcg gttgaagtat
    14161 tggcagaaac tacagacaaa gcacctggta cgatcatccg gaaagacaaa aaaaatctat
    14221 ggattgcttg cggtaaacag accgtcttag ccatcaatga attacagcca gctggtaaag
    14281 gaaaacaggc cgtacatgat ttccttaacg gaagcggtca gcaagttcag attggtcagc
    14341 aggtgaaata atgaaaaaaa tccctaaaaa aatgaaaaaa tcagtacgat atatggcatt
    14401 actgacatta gagcgagtga acaagggtgg cgcctactcg aatcttttgt tgaatgagat
    14461 gatcaaacaa agcgagctaa atcaaaaaga tgtgggttta tttacagaat tgatctatgg
    14521 tacgattagc catcagcgac tattggaatt ttacgttgca ccattgatcg ctaaagcaaa
    14581 aaaagtggat gactgggtca agatgcttct ttatctttct gtataccaat tagaattttt
    14641 agataaagtc cctgcacatg ctattttaaa cgaggcggtc gaaatcgcaa aagtaaaagg
    14701 aaatcctggg acagggaaat ttgtaaatgg tgtcttgagg aattatcagc gtcaaggagc
    14761 accagatctg caaggcattg ctgatcctat cgaaagactt tctgtcaaaa tcagcctgcc
    14821 tctatggatg acgaaacgac tggtggatca aatcggatac gaagaaactg aaaagctcgg
    14881 tttatcgcta tatcagccta gccatgctag tgcccgagta gatacacgcc gactttccag
    14941 agatcaggcc atcactgttt tgcaagaaga agagatcaca gctgaaaaaa gccaagtcag
    15001 tccatacggt atagtagcga aaaaagggca tttagcttct agctcattgt tccacgatgg
    15061 cgtcatgacg atccaagatg aaagttcgat gcttgtagcg ccgtctatgc aaatcgagcc
    15121 tgaacatcat gtcttagatg cctgtgcagc acccggaggt aagacgacgc atattgcttc
    15181 ttttttagat ccgtcatgcg gtggagaagt ggtcgcttta gatgtacacg aacacaaagt
    15241 caaactcatt gaagataatg cagcacgtct aggtgtctct tctgctgtct acgctcaaaa
    15301 gatggatgct agagaagtca gtaaacagtt cgaagcagag cagtttgatc ggattttagt
    15361 agacgcgcct tgttcaggtc ttgggctgct tcggcgaaaa ccagatattc gttataaaaa
    15421 acagccggat gaattagcga atttaccaaa aatccaattg gaaattttag aaagctgcgc
    15481 accaacttta aagtcttccg gtattctgac ttatagcact tgtacgatct taaaagaaga
    15541 aaaccaagac gttgtttctg ctttcttgga aagacaccct gattttgaga ggattgatgt
    15601 tttgaccaat cctatgctac aatcatcaat cgaagagaaa atgttgactt tgtatccgca
    15661 tcagttttac acagatggat ttttcatttg ttgtttgcgc aaaaagtaat gaggtgaaaa
    15721 aatgcagatt gaatatcaat cagatgtagg aagacgacgt aacacaaatc aagactatgc
    15781 cagtgtattt accagccgtt caggtctcaa gcttgctgta ttagcagacg ggatgggtgg
    15841 tcatcgtgcc ggagacattg ccagtcagat ggcggtaacg aatctaggcg aagcttggga
    15901 aaaacaagaa gctgttgacg atgaaaaaat cgcccaatgg ttcattcaat cgattcaaga
    15961 agaaaatatg ctgatttatc agcgtggaca agaacagccg gaatataacg gtatgggaac
    16021 aacaatcgta gcggccgctc tgtcagatga ctggttcacg attgccaatg ttggggatag
    16081 cagagcttat ttgattcgtg aagataaaat catgcaattg acggaggatc attctttagt
    16141 caatgaacta gtcaaatcag gagaaatcag cgaagaaatg gcagtgaacc atccaaggaa
    16201 aaatatttta acacgttctg tcggcatgcc tggaacagtc gaagtagatg tttctactca
    16261 tctttggcaa ctaaaagatc gactgctgtt atgttcagat ggcttgacga acatgctgtc
    16321 tgaagaagaa ataggaacga tcatcaatca agaaggccca ttgtctcata aagtaactga
    16381 gctgattgat cgtgccaatg aagcaggcgg agcggataat atcaccgttc ttcttatcga
    16441 atataaggag gacagtgtat gatcgagttg ggcaaaaaat taaatggccg ttatcaaatc
    16501 actggaaata tcggtagcgg tggtatggca aatgtatttt tggcgcacga tttaatcttg
    16561 gatcgtgatg tagcagttaa agtcttacga tttgactttc aaaatgacca gactgcgatt
    16621 cgacgatttc agagagaagc tttagcagca acagaactcg ttcatccaaa cattgttagt
    16681 gtttacgatg ttggtgaaga agacaacatg cagtatctgg tgatggaata cgtcaaagga
    16741 atggacttga aacgatatat ccaaacacat tatcctgttc cttatgaaac agcagtaaat
    16801 attatgcagc agatcctttc tgctatttct ttagctcata accatcaaat catccaccga
    16861 gatttgaaac cccaaaacgt actgatcgat aatgaaggtg ttgtaaaaat cacggatttc
    16921 gggattgcta tcgctttatc tgaaacatcg atcacgcaga cgaatacgat gctaggatct
    16981 gttcattatc tgtcccctga gcaggccaga ggaagtatgg caacgaagca gtcagatatt
    17041 tatgctttag ggatcatcct ttatgagatg ttgacgggta gcgtaccgtt tgatggtgaa
    17101 tctgctgtaa caattgcttt aaaacatttt caagatgatc ttccatctat aaaagcactt
    17161 gatccaaatg tgccacaagc actggaaaac gtgattttac gggcaacggc taaagaacct
    17221 gcagaccgtt ataagtcagc cgaggaaatg tctgatgatc tttctacagt attatcacca
    17281 gctcgtgcaa acgaagaaaa atggcagcct catgtgatgg ataatgaaac aaaagttatc
    17341 acacccctta cggaagatgc accaatgcct gattcattca aatctatgcc tctgccaaaa
    17401 gacaagccag aaaacgaaac cttaccagag gaggaagaag ttctgcctga tcctaaaaag
    17461 aaaggacaga aaaaatggtg gctgatccta ttagctattc ttgctgtttt aggaatcgga
    17521 acagctgtat tctttgcttc tggcgggcga ggagaagttg ttgttcctga tgtttctgat
    17581 ctttctgaag cttctgctag agaaacactc gcaaaagcga aattagaagt agcaacaaaa
    17641 acagaagaaa ttgcagatga caaaatcgaa gaaggtaatg tagtgaagac agacccggca
    17701 gcgggaacga cagtcaagca aaaacgcgaa gtcactctgt atattagtag cggcacgaaa
    17761 aaaatcaaat tagatgatta cagtggtaaa agttatgaag atgccgaagc aagtttgaaa
    17821 gaattagggt tttctgctga cttgattcaa aaagaagagg aattcagttc agaggtagaa
    17881 agcggaatga tcatttcgca atctccatca gaagatacag aagtcgatcc taaaacagac
    17941 acgattactt tcacagtcag caaaggacca gaaacagtga ctttgagtag tcatgtagga
    18001 gaaaactacg aaactgttcg taattggctt gttacacaag gattcaatat caataatatt
    18061 agtgctactt atgactatag cgacactgta ccatcaggtt cgatcatcag tcaggatcca
    18121 gcctcgggtg aagttgtagc agaagaaact tacatcaatt ttgtcgtaag ccaaggaaca
    18181 gagcctaaat tgaatgatat ctccggctat acaaaaagcg aagcacaaag ttatttagct
    18241 agtgtcggtg cagaatatat cgggcatgag acttccgaat attcaaacac ggtggataaa
    18301 gataaagtga ttcgtacagt accaggggca ggaacgacaa ttaccaaagg aatggttgtg
    18361 aacgtaattt actctaaagg accagatcca gcgtcttcta gttctgaaaa agactcttca
    18421 gacagttcta gcagctcaag tagctccagt agcacaagca actcttcaaa agaagaatct
    18481 tccagctcca caagttcatc agctagcacc tcagaaagtt caaaataagt gctgaataaa
    18541 atatatcaga agggaatgcg acattgattt gttgcattct cttttttgtg tttctatctc
    18601 ttttgatcaa ttttcaagcc atatactttg agaagtttgc atggaataac acctactttc
    18661 ctcttcgtat ggtaaaataa acaaaaacgg aggtggcact tatcaaagga caaatcagaa
    18721 aagcaatcag cggtttttac tatgtatacg caaacggaga gacctatcaa acaagaggaa
    18781 gaggaaattt ccggaatcgg aaaatcacgc cgcttgtcgg agatcaggtg atctttgaga
    18841 gtgaaaatca aacagacggc tatcttctgg aaatcttgcc gagaaaaaat caattagttc
    18901 gcccaccagt cacaaatgtt gatcttggaa tcattgtcac aagtatcgta gagcctgcat
    18961 tttcttataa tctacttgac cgctttctag ttaccttgga atacgagtcg atcgaaccga
    19021 ttattttttt gacaaagacc gacttgtcag acgacccaga aaaaatcgaa gagatcaagc
    19081 aaacctatac taaaataggc tatccggtga ttgtacccaa tcatacgggt gatacgacag
    19141 aactgatccg ttacttccca gaacggctat cggtctttat gggtcagtct ggagcaggaa
    19201 aatcgacgct actgaatcaa atatcaccgg aactgaattt agcaacagga gagatttctg
    19261 atgcacttgg aagaggacgg catacaacac gtcatgtgga actgattcct atctatggcg
    19321 ggttagtagc agatacacca ggatttagct cgattgactt tttgacaatt gaagcaggag
    19381 aactgccgag acaatttcct gaatttgtag aagcttctgt ccattgcaaa tttagagagt
    19441 gcatgcatgc taaagaacct ggctgcgaag taaaacatag agtagaaaca ggtggtatcg
    19501 cacagacacg ttacgacaat tatttgcaat tcttacaaga aatcgaaaaa agaaaaccaa
    19561 tgtatgcaaa aaaagaaggt aaacgaaaat aggaggaaac aagaatgaag atagcaccat
    19621 ccattttgag tgcagatttt gccaatttac aaagagacat tgagttagtg gaaaaaggag
    19681 gcgcagatta catccacgta gatgtaatgg atggccgatt tgtcccaaat atcacattag
    19741 ggccaaatat tgttcaggca atccgtcctg ttactaaatt accgttagac gtccatctga
    19801 tgatcgttga tccagaaaac tacattccag catttgcaaa agctggagca gatatcatta
    19861 ctgtccacgt cgaagcaact cctcatattc atcgggcact tcaaatgaca aaaagcttag
    19921 gagttaaagc tggagtagtt atcaatccag gaacaccagt ctccatgatc aaacatgttt
    19981 tgcctttggc tgatcaagta ctagtcatga ctgtcaatcc tgggttcggc ggacaaagct
    20041 ttattgaaga aacagtagaa aaaattacgg aactcgctga tctacgtaga gagaacaatt
    20101 ggacttatgc aatcgaagta gatggcggaa tcgttccaga aacagcaaaa atctgtaaag
    20161 atgcaggggc ggatgtcttc gttgcaggat catatatcta tggagccgaa aatccagtcg
    20221 gacaaatcga acgattaaaa gaggcattag actaaatgaa aattttatta gctgctggag
    20281 gtaatccgca aaactggccg cagtctctgc tgtccaaata cgatgcctat gtcgggatag
    20341 accgtggtgc gctgtacctg cttcaagcag gctacccagt agatctggca gtgggtgatt
    20401 ttgattcatt gtcgaacgaa gagcagaagg aagtatttgc aaaagccaaa gagtataccc
    20461 gttcccctgc agaaaaagac gatacagata cacagttagg tatcttaaaa acatttgaac
    20521 gttacccgca agcggaagtc actttgattg gagctacggg cggaagattg gatcatcttt
    20581 tagccaatct ctggttaggt atggaacctc gcttcaaacc ctttgcccat cagcttatta
    20641 tcagagattg tcaaaattat ttgtcttatt atttgcctgg agagcatata gtaagacaga
    20701 taccagatat gaagtattta gcttattgct gcttgactcc cgtctcgaat cttacattaa
    20761 aaggcagcaa gtatgtattg gaaaataaag aagtagaata tccgacatct tatgcaagta
    20821 acgaatttct ttcagatacc gcagaatttt ctttttcaga tggtatgatt gcagttattc
    20881 aaagcaaaga ttgatccata aacaaataaa aaaatttgtc tttcaggcta aaaatgacag
    20941 aatctattat ttgctaaaaa aaagcttaag aggagcaact taccttttgt ttcacggcat
    21001 ttagtgataa aattgaacac aagagtctaa ttaattcgag gtgtctttgt gaagaagttc
    21061 aatcctaata aaaatattat tattaccttg gttattgtta tcatcgtggt gacgatacta
    21121 agtgtgaccg ctgcgcagcg agcgaatgaa ggaaagccca atttcttcca atccatcgtt
    21181 aatgacagtg ttgcaatggt tgataaaaca atctctgccc cagtacggtg ggtacgaaat
    21241 ggagtagatt ctgtccataa tcttttttca acttatgccg aaaatgagcg gctaaaagaa
    21301 aaaatcgata gctatgatga cgtttcacag caaaacaaga attacgagag agaaatcgaa
    21361 gcgttgaaaa aagaacttga gttgaatcag acgctgacta gttacgaaaa agtaacggca
    21421 aatgtcatta ctcgttcgcc tgacagttgg caggacatgc tgatcgtaga caagggttct
    21481 aaagacggta tcgaagcaaa tatggcagtc atgtcgcaaa aaggattgat tggccgagtg
    21541 atcgaggtca atacggcttc gtctaaaatc gaattactgt catcctctaa tgaaagctcc
    21601 aatcattttc cagtacgggt atcttcggct aatggcgaag cgtttggttt gcttaaaaac
    21661 tatgatgaaa agctccatgc cttagtggtg acccaattaa ctggtgatac ggatatcaaa
    21721 gaaggggatg ttgtccagac atccggtctt ggagggaatt ctccagctaa cttgccgatc
    21781 ggtacggtta ttaaaacgaa accagatagt tatgggctgg atcgggaagt ttatgtgaaa
    21841 ccttatgcag aaatgtatga cgtgtcagtt gtgacgattg tccaacggct agcggaggat
    21901 tagtccatgt tgaagaaaga aacaatgaag tactatctgc caatcgtttt gttctttttg
    21961 atgttgatag atggtcattt aacaagaatg ctaggggagt ggtcgaaagg cacctatatg
    22021 tcaaatgccc actttctgat attggcatta ttatgttgca gtatggcgtt tgaaaaacgt
    22081 tatttactga ttaccacgat tgttctcggg gctatctatg acgcttacta tattggcgtt
    22141 atcggtatct atgcagtagc tctcccttta attgtatggt tgatgtatgt aatgaaagac
    22201 gttatccatg tcaacatctt tactgaattt ttcagtatga tcatctttgt cacgggttat
    22261 gaattgttta cgatggtggt ccagttgatt tttaaattag cagtagtaaa taacacgtat
    22321 tttattacaa ggtttttagg acctacact
//
LOCUS       DADXTG010000038        22315 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.038, whole genome shotgun sequence.
ACCESSION   DADXTG010000038 DADXTG010000000
VERSION     DADXTG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.038"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(49..381)
                     /locus_tag="IWI57_001205"
     CDS             complement(49..381)
                     /locus_tag="IWI57_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449364.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(395..652)
                     /locus_tag="IWI57_001206"
     CDS             complement(395..652)
                     /locus_tag="IWI57_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449365.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(847..1389)
                     /locus_tag="IWI57_001207"
     CDS             complement(847..1389)
                     /locus_tag="IWI57_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449366.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1410..2501)
                     /locus_tag="IWI57_001208"
     CDS             complement(1410..2501)
                     /locus_tag="IWI57_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBC2449367.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNGE
                     YTEGLPIFSEIRGRGSITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKMK"
     gene            complement(2509..4719)
                     /locus_tag="IWI57_001209"
     CDS             complement(2509..4719)
                     /locus_tag="IWI57_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449368.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVINPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQQLGQRSSESKPSISAP
                     KSLQPAFSSLDKSTDQSKKIEKDPKTLPILDAKLGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4735..7263)
                     /locus_tag="IWI57_001210"
     CDS             complement(4735..7263)
                     /locus_tag="IWI57_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2449369.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGTSYLALLPIGETPENMRSCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSFVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7316..7726)
                     /locus_tag="IWI57_001211"
     CDS             complement(7316..7726)
                     /locus_tag="IWI57_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449370.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVAI
                     FEYLFLRSFISWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7739..7957)
                     /locus_tag="IWI57_001212"
     CDS             complement(7739..7957)
                     /locus_tag="IWI57_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449371.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNNPTRVFDAFGKIIEKLFMN"
     gene            complement(7954..8946)
                     /locus_tag="IWI57_001213"
     CDS             complement(7954..8946)
                     /locus_tag="IWI57_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2449372.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKGVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKIDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDTKGEERK"
     gene            complement(9025..9939)
                     /locus_tag="IWI57_001214"
     CDS             complement(9025..9939)
                     /locus_tag="IWI57_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2449373.1"
                     /translation="MIKTTLAVVTTVGITLFSFQLGTLLVNADETVETPKSEIVDTLP
                     GEELPDVETELSGGASETPEQSQPEQPEKELIESEPEFPESTLPTDPIEEEGVHGSTT
                     TDSSTVTTPEQSGTTSSSEKTTDSSSTEQPTMSSTSEESKEEPSQPAEKASEKSEAIP
                     KENKPVVPAKEVTVSVTPSGEITTNTSQGTSVPIVTSNVEELTHIPTPTTPLKVESGQ
                     TIVGVKDGIPLVQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVL
                     SVLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9958..10303)
                     /locus_tag="IWI57_001215"
                     /pseudo
     CDS             complement(9958..10303)
                     /locus_tag="IWI57_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10323..10880)
                     /locus_tag="IWI57_001216"
     CDS             complement(10323..10880)
                     /locus_tag="IWI57_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449374.1"
                     /translation="MSLEFLGEPIEKEEFVLHYMDLFIQLIKKLCAIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFIKREKNQMSVEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10960..11460)
                     /locus_tag="IWI57_001217"
     CDS             complement(10960..11460)
                     /locus_tag="IWI57_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449375.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKRELEGQFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11461..12006)
                     /locus_tag="IWI57_001218"
     CDS             complement(11461..12006)
                     /locus_tag="IWI57_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449376.1"
                     /translation="MNSYMTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLESIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLATVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12003..13259)
                     /locus_tag="IWI57_001219"
     CDS             complement(12003..13259)
                     /locus_tag="IWI57_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBC2449377.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKRKIPEDFEQRIKERFRLYVESGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(13619..13930)
                     /locus_tag="IWI57_001220"
     CDS             complement(13619..13930)
                     /locus_tag="IWI57_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449378.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVGLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13953..15263)
                     /locus_tag="IWI57_001221"
     CDS             complement(13953..15263)
                     /locus_tag="IWI57_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBC2449379.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMRWSRLKEGYFASVYWRQLLA
                     RMLIDNRFIYTKKQVGEKQMKEKMRLPKVYFYQKKQELVISFPLDGGRFHERFLKMGN
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     LLISGAIGGGKTYLLYSLIKACLDVGTVDICDGKAADLAALGDVDIFKGHVFYKTNEE
                     MIACLRNALKEMNQRYAYMKTMKEPKYEPGKNYAYYGISPHFIFFDEWAAFYGSLDME
                     TRNKVDKLIQPLVLKGRQAGMYVILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15432..15809)
                     /locus_tag="IWI57_001222"
                     /pseudo
     CDS             complement(15432..15809)
                     /locus_tag="IWI57_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15878..16330)
                     /locus_tag="IWI57_001223"
     CDS             complement(15878..16330)
                     /locus_tag="IWI57_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449380.1"
                     /translation="MGLKFPNNYDITEALDETCVGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNLKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16332..16697)
                     /locus_tag="IWI57_001224"
     CDS             complement(16332..16697)
                     /locus_tag="IWI57_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449381.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(16796..18820)
                     /locus_tag="IWI57_001225"
     CDS             complement(16796..18820)
                     /locus_tag="IWI57_001225"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBC2449382.1"
                     /translation="MVFSITNDTEPIKVIHFANRQARKNVKLFKYEEWNKKKTPLAGV
                     TFTLYNQAGKALGEYITDQNGEILVKGLVANNYYFIENTPLESFQPNTDKQAFTITFK
                     QNAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGK
                     EYTISGVLMNKATNTPILQDGKEIRKTLTFIADKVNGTKDLEFVVNASVLKGQTIVVF
                     EKLFRDGIEVAAHANINDENQSVRVNNPDVHTTAVFEDGLTVADPTGLFTLNDMVKIK
                     EIIPGKAYLVKGILMDKATGEALLVHGQPVESAGGFIAKAENETVIVPFTFDLTGLNG
                     REIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKVVNPLMKITI
                     TDAVKYHDLIVGKTYTVNGILMDKSTEKPVLVDDKEITSTLTFVAERIDGTVNLDFVL
                     DARALRGKEVVVFEDLFRDGVLLASHADITDKDQTIKIVNPTITTKATNKNGGKEILA
                     LPNQTVLEWVKVDGLAIGQLYKLLVQGYLTPDGTPFEGTHAEKVFTADKEMMEFLFEF
                     LVDGRNLAGKGLSFVEWLQAKTEDDKEAFEEVVKHNVDLTNKDQTVQFVEAPKPPQPV
                     KPEQPVKAATVVPMLPKTGEQTSSILMVLGLVVLVIVLGITLYKVRKAKD"
     gene            complement(18778..21078)
                     /locus_tag="IWI57_001226"
     CDS             complement(18778..21078)
                     /locus_tag="IWI57_001226"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBC2449383.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAKGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETSVKAQGASSLNNAVYGLYQADGKKIKEVTL
                     KNVNGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLIDSMNPDNKPKLAGIEISLTSKTTGKVVKTVVTDKDGYATFGKNAV
                     VFDTYILSETKGKEGYKLFEPFEVTISENGQTFHYALEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTFTYGKDRYRLEE
                     IKAPVNYVLNQNHWYSRLPMIQNQSK"
     gene            complement(21236..21463)
                     /locus_tag="IWI57_001227"
                     /pseudo
     CDS             complement(21236..21463)
                     /locus_tag="IWI57_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21553..21825)
                     /locus_tag="IWI57_001228"
     CDS             complement(21553..21825)
                     /locus_tag="IWI57_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449384.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21908..22159)
                     /locus_tag="IWI57_001229"
     CDS             complement(21908..22159)
                     /locus_tag="IWI57_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449385.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6519 a         4858 c         3336 g         7602 t
ORIGIN      
        1 tcataatttc tcctccataa aaaataagtt actttttaaa tatcttgttt atgaattcat
       61 tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag acttgtcata
      121 caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca atccattaaa
      181 cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa tttttatacc
      241 ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt gaatttcttg
      301 cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg attcaacgat
      361 ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag ccatgtagag
      421 cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt actctacatt
      481 ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca atgttcggat
      541 tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat acaaacaatc
      601 ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc atctcgtatc
      661 catctcctaa aaaatagaat agttcttata gttattcata agacatattt tttccgtttt
      721 tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca tagtttttct
      781 taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga gtgttcattt
      841 tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct tttgagcttc
      901 ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg tttcttctcc
      961 aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa gtttcccagt
     1021 tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac aacgatctgt
     1081 catataatcc ctcacagctt gattgcgttt ataaacatct gtgaaattac tccattttct
     1141 agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt gcgctgtctc
     1201 gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc cacttgtttc
     1261 ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt tttcagaaga
     1321 aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta aaattatccc
     1381 ttttttcacg tttctagtca ctgcctttct tatttcatct tacgaaccag actacgaatt
     1441 tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc ggcattctgt
     1501 tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc ggtgtgacca
     1561 tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc tgcttgtaaa
     1621 tcacttggtt ttgggttcat gatcacttgc caaccatagg ctgtccaatc ataatcttcc
     1681 cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc tagttgggat
     1741 acataccacg ccgtgccacc ataacactct ccattattta ccgatcgtcc aatgatccga
     1801 tctaaaactt tcgcattacc tttacttttc cctgcaacaa gtgacgattt ttttgttggt
     1861 aggctattcc aatgtttgat cgcaaaagta tcatattctg ttaagttata agactttatg
     1921 atactcatcg ctccttggta atacggactg ccttcggtaa tacctgccgc cgcatagcct
     1981 ccacgaccta gaattaataa acaactccca ggatctttat tgcccacaca ttgattaaag
     2041 ttgctttgac tcgccaccat aaagccgtaa tagtcataac aagcttttag attcggaaac
     2101 ttcatataga agccgccttc agaaccgcca atcccccgtg cagaaccttg tggataggga
     2161 cagcctggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga ttgtgactgt
     2221 ccataagccg attcataacc caattgagaa agaatcacac tcggcaacaa ctgatacttt
     2281 accgccgcac caactgctgc ttgcgcatgt tcatctgtga tcgaaccacg tcctctaatc
     2341 tctgaaaaaa tcggcaatcc ttcggtatat tccccattaa aagcgacagc tagacctgta
     2401 gcctctcctc catcctcgga aagttctcct gaaaaaaaga atggcaatag aaaaaaggga
     2461 agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc atcctttctt
     2521 cgacaatggt ttttggactg ttcgtccttt tggtaaagta aaatcggttc ctagttttgc
     2581 atccaaaatc ggcagtgttt taggatcctt ttctattttt ttcgattgat ctgttgactt
     2641 atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg gtttactttc
     2701 agacgaacgt tgtcccagtt gttgcacaga acgatttcct gatagagtgg ctaccgacga
     2761 cttaggaaca gagtattgat tcaataactg ctgcattgct ggataactcc catttttcgc
     2821 ctgatttttc acttggcgaa tctgttggga aagagaacca gcagctgtta attgtggcac
     2881 gggtactttt ccagcaggca acggaatcga ttgaccaaca aaagcgatac ctttatttaa
     2941 caatggtttt ctttgattat tcccttgttg cttactcgat tgtgccaacg attgtacctg
     3001 ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg aaagaccaga
     3061 attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat gataacgaac
     3121 atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa agggattaat
     3181 cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact tttgaaatcc
     3241 atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg ctaatcctgc
     3301 acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt gtttcatttg
     3361 agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg cagctcctaa
     3421 ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata ggagtgcttt
     3481 gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat cgtctactat
     3541 gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt gaaacatcaa
     3601 gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa caaaagaagc
     3661 gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa agataaaacg
     3721 aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg gcaccataga
     3781 accaacgact gctttataaa cagctttagt tggttgaata tagacacgat aagccttctt
     3841 tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag ataattcttc
     3901 acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat acaattcttc
     3961 taaatttgtt gtcccataat ttaacaatag atacggatca acaacagccg ctttataata
     4021 aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa caccattgac
     4081 atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc cttctacctc
     4141 cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta cgttactacc
     4201 atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa acaccatttt
     4261 tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat acaagacgac
     4321 aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa aagtgaaaaa
     4381 ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga caaaatcaag
     4441 gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt ttagaaaatc
     4501 tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag ataaataaga
     4561 agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca cctgcttttc
     4621 tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta aaaaaaacaa
     4681 aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc atttctattg
     4741 catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg ttgtttgtaa
     4801 tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac gtccataggg
     4861 atctaacatc caacactgtc ctttaatcat atggctcatt tcagataagt tcgcctcatt
     4921 tacttccaaa cgtaaatgag tcaaaatctt ttctcgctca ctgtcttcat caaaagcgaa
     4981 gacaacacca aaattccctt tttctttatc tgaacctaag tcgccaataa attgtgtatc
     5041 taacactaat tgattcatct gtgaacgccc cacacgcgcc attgctgcaa taatactacg
     5101 tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg ttttcttact
     5161 actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca aacacaaaga
     5221 ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta atttcaaatc
     5281 ttggatctct aaaatcgttg ttttggcatt aaagtctaat cctgggtttt ctccataaga
     5341 aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac catattctcg
     5401 aacctctttt tgttccattt gttctaaata ctcgaccaca tgcatcaagc caacttgttg
     5461 cccggcttct ttttgaacta aaacttcttg gatccccttt aataccgccc cttttaaaag
     5521 gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt gtttcgcatc
     5581 tactcgggat aaacaaacaa ttggatctaa tacgccataa ttacggggat ttgtagcatc
     5641 taacgtgaca aaactcattt tttgcaaatg ttccacaaac aaagggtaat gtttctgata
     5701 atacgggtct tgaatgagtt tcgtgaacca tttgcgcttt tctcctttgg gatctacaaa
     5761 taaactttgt acattcataa acgaactata caaaaagagc aagccaacta aaaatgtttt
     5821 ccctttacca gtttttccag ttactgctac atgaggcgca tcaaacaacg cacctttgat
     5881 tccttccgca atggctaaaa gatttaaaaa gacaaagcga tttgacgcgt agatcaattc
     5941 ttctaacggt gttttctctc ctctagaacc accgtcccct aatgtatctt gacgaccaat
     6001 ataccagcca gattccatac ctaactctga tactgctgaa aacaaccatt caccaactgc
     6061 ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgtc ctaataaaca
     6121 tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa tcgtatgaag
     6181 ctgttgaata atcgttgcac tattttgacg aacttcttca accgtttgac cgtatacaat
     6241 aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct ttaaatgttt
     6301 tactaatccc cgaacagtta ataagcgttc actaggttct tcatcatttt gaaccaattc
     6361 cttatgttct gttttgaacc gttccgatag ccaattcagt tttaaaccaa tacctccctg
     6421 tcctttcaag cgtggatagt gaattttcat tcgtaattct gctgggaaag aaagatcctg
     6481 tgcaaattga aatagatcac aacttctcat attttcaggt gtttctccaa taggtaacaa
     6541 cgctaaataa ctagtcccca attcatcttc taattgaaga aggcctttat gttccttcgg
     6601 tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt gttcttctac
     6661 tttatgagcc attcctcgaa tatattgcag acgattgaga tacaacagct ctttttttac
     6721 taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat aaggatcact
     6781 ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg ctaaacttcc
     6841 tgctacatct aaccagatta gatttaatac atcatgaaag ctctctaccg ttgctttttc
     6901 tttgactttg attcctaaaa taaatgaagg acgatagact tgcccttctc gttctaacaa
     6961 tgtcactgtt tcttttgcat aatacgcacc aatggcttga ctatcaggtg aaaatccctt
     7021 acctaaggtg tcaaactttg ttcgtaaatt cattgttcgt ggatacatca ccaactccac
     7081 atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta tttcctgtgt
     7141 cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg cccatataca
     7201 atcctcgcca tttaacaaac aatttttctt aaatgctttt actggttctt ttacaagcac
     7261 caataagttt cacctcccaa taaaaaaagg aacaaccagt tgattgtccc tttttctaat
     7321 cctctaaata aacatactta ttcacaaaaa gtaattcttc caatgcattg tttgatacct
     7381 tgtcccagtt ataatacaca caatcaccat tcatcatacg cactacataa caaaaccaat
     7441 cccataaata aaaatgcact ttttttccat caggttttag ttttgccagt gctaacacac
     7501 ataaataggg tagccctata taaaaaacaa ctcctgctgg tggaaaaaaa gaaataaccc
     7561 aactaataaa cgaacgtaaa aataaatatt caaagattgc gaccaacaaa aaggttgctg
     7621 cattgactaa tgcaactgga aatggaagac gccaattgct tgtaatctgt tgtacgcgat
     7681 aaggttccgc ataaacaatt gaataatcat aaggttccca atccatgttt ctattctcct
     7741 aattcataaa taatttctca atgattttac caaacgcatc aaatacacgt gttggattat
     7801 ttaaaaagaa atagactgct gcacctgctg caatagatat taatgcattt cctacttttg
     7861 atttcataaa atttcgtaac cccagataaa caaccaccat tattaaaaag tagccacctt
     7921 gcgttaatat aaaatctaca attcccttca gtgtcatttc cgttcttctc ctttcgtatc
     7981 caaccaatta tgtgttagct tttcaacata atactgattt gtttttttcg taataacaag
     8041 cgtcatttgt tctttttgta aaagtgacgt atcaccatct cgaaagacga ccgttgcttt
     8101 tacaataaaa ggttttttcg tttctttata acgcaatact ttcatctcta cttgatcaat
     8161 tttagctgtc tgattgagtc cttcaggttc tttcatcatg tatgacatat cttttgcatt
     8221 gtttttcgca tacttatcta agaaatcttc cacaaatcct tcaacacctt tcgcttccga
     8281 cgcatcaacc gtttcgtata atgtgtcatc ataagtaatc attcgttgtt tttctgtatt
     8341 ctttggtagt gccgtaaaat aaggataagc aataatacga cagcccttgt ttgaaataga
     8401 aaagggaaga ttgagtactt gtgtgattgt cacacttgtt tctttcttac tctctggatt
     8461 catcgagcga atatcataag ttactttata actagctgtt ggaatggttt caatttcttt
     8521 taatccaata aacgaagcag cagttaattt tcgaacacct gaaacacttc ctggttcttg
     8581 aataaagttt tgcgcataaa actctttttt taatgtttct tgacgttgtt gttcttgttc
     8641 atgattatcg aatgtattca tatacaaagc aacaaaacga ttcataaagg attcaatttc
     8701 aggtgtgtac gttaattctg accgactttg tacttctttc ttttgtaatt gttggatttc
     8761 atctttcaaa aacgacactt gaaccttcgt tttcatggat tgaaaaaatg ccaagccccc
     8821 tgaaaaacaa agaaataaaa ccaaacacca aatccatttc actgcttttt ttgaatggat
     8881 tttcttttgc ttcggtactt tctctgtata aactttttct ttccgccgta gttgtcgttt
     8941 tttcataagc tactccttct agtatgtttt tactagtacg tacgattgac aacgataagc
     9001 ttgtttttct gaaaaatagt ccattcaatt accctcattt tctattcctt ttctctttct
     9061 tttatatcca acaaatccag caatacttgt caacactcct cctagaacag aaagtacaac
     9121 atgtatttct tcaccagtct ttggtaagac ttttgttttt ccatctgctg ttttgacttc
     9181 aatattgcca cttggaagtt tctttacagg aatcgacaaa tcctttacca aattcccttc
     9241 actatcttgg actaatggaa ttccatcttt tacaccaaca attgtttgac cactttctac
     9301 ttttaatggg gtggttggtg ttggaatatg ggtcaattcc tctacattac tagtgacaat
     9361 cggtacactg gttccttgag aagtattcgt cgtaatttca ccgcttgggg ttaccgatac
     9421 tgtgacttct tttgctggaa caacaggttt attttctttt ggaattgctt ctgatttttc
     9481 gcttgccttt tcagcaggtt gactaggttc ttcttttgat tcttcactgg tagacgacat
     9541 cgttggctgt tctgtacttg acgaatcggt agtcttttca gaagaactag ttgttcctga
     9601 ttgttctgga gttgtgactg tacttgaatc cgttgttgtt gagccatgca caccttcttc
     9661 ttcgattgga tcagtcggta aagttgactc aggaaactct ggttccgact ctattagttc
     9721 tttttcaggt tgttctggtt gagattgttc tggagtttca cttgctcctc cacttagttc
     9781 tgtctcaaca tctggtaact cttcccctgg caatgtgtct acaatttctg acttaggcgt
     9841 ttctacagtt tcatctgcat ttaccaataa cgttcctaac tgaaacgaaa atagtgtaat
     9901 ccctactgtt gttacgactg ctaatgttgt ttttatcatg attattcctc caatttttta
     9961 tgcaattcgt gttttgttca tagacgccgc actaatatta tctgaacgat aaaagaactc
    10021 tcgtccgttt tcttctaacc caacatatgg attatttttg aataattgaa tcaatagttc
    10081 actggtgttg tattttggat agtttgcaaa aaaaataata ctgtgcttgt cgcccatctg
    10141 aaaattcaac aagtaattca tagcgatacc gcttcaatcc ttttcgttgt tttaaaggat
    10201 ctctatacca acttgtccat cgttccattc gtggatagag atagacacga tacacataga
    10261 acaatgccat accgcatacc accaaaaata ataatcgcca cataaaaatt cccccttttc
    10321 ttctaccttt cttgaatcat cgaacattca taccctctat agagatttga aaaatccatc
    10381 atcaattgtt catataaacg ttgttgcaaa tagcgattgt ttcgaataca taattcttca
    10441 tagccatcaa tcacttgatc ttctttttca tagcatcttg tagctctatg acgctcagct
    10501 tgcatgacat aaccaaactc tgcaccatat cgttctaccg ttaataactg ttgactacgc
    10561 aataatgcaa tataacaatc acaaaaatgt tcaaccgaca tttgattttt ctcacgtttg
    10621 ataaatagat actctttgga aagaacatta ttcgttaaac aatctgataa atagcgataa
    10681 tctggcggtc ggttattttc atctgtgaat ttttcccata ataaactgat ttttattcgt
    10741 tcagcggcta gatagtcttg ttcgcgcgga taaaattcat caatcgcaca aagctttttt
    10801 attaactgaa taaacaaatc catataatgc aaaacgaact cttccttttc aattggctct
    10861 cctaaaaatt ccagtgacat accatcttct gcttccttaa aaaataaaaa agcagaaacc
    10921 gatttggctt ctacttttct ttccttttat agctcttttt tacggaagaa tccgcaaatg
    10981 acattttttc aaaaaatcaa tcggcacaag ttcacgaaaa cttggagcaa ttttggatag
    11041 aatcacttcc ccatcttgta aaatacaatc ataaaaatca tcagttactt gatacttacg
    11101 cttcttgcaa taattgataa aatagtagcg atcattttct atttccattc gttgttctac
    11161 taagcgcata cgaacatcat catagaaata agccacaaat tgcccttcaa gttcacgttt
    11221 ttcaatggga tactcttctg gtaacaatct attttttcgt cgtttataca ttgctggata
    11281 ccttagaagc agttcatcta aacgtctgtc agtatatggc aactgccgaa tcaacgattc
    11341 taatcgttct ttatacagat agaataattg gcgcaaacaa tagtgtactg cctctggatt
    11401 ttctaataca gttggatcac gtgaccaata cgcaccaaca taaatcataa tttttttcat
    11461 ttagacaatc acaccagcaa tactaattac cgatccacaa taggtagaac aagtggcata
    11521 gcgaccaatt tcctctaatt ctatctcaat ctcttctaaa gaaagatatg tctgttcttc
    11581 tgttggctgg cgactcattt cccaagtgac agtcgccaat gaaatttctt ccataagagg
    11641 tttttcgctg ctaagtgctg ccacacagtt ttcgtatatc ttcattttat cgcagccttt
    11701 tttaaccgct aaaatcgcag gtattggcaa tgaaaatcga taaaagcgaa tggattctag
    11761 cctacctttc cttcggctaa attccaactc ttcgagttct tgttcgtatc ccttacgaac
    11821 ttctaataag cgtgcaattt cttgccagtt ctctttttcc tgataacgtc cagcttttac
    11881 cgcttgttca atataaggaa ctcgaaaacg aaaataagac aaatcaacca tcttttcatt
    11941 taatgcttcg atcgcagctt ttaaatccaa caggcgcaac cattctgttg tcatatatga
    12001 gttcatgcaa ttttccctcc tgacgttccc atccattcta gatagctttg ttgctcgtca
    12061 tttagttcag cattatcaat gccttcttgt acaaagtctc ttcctaaaat acgatctgct
    12121 tcttgtaaca attttaatgt cggagctact tgtttcaaaa accagcgttc tgaacgatct
    12181 aatgagaatt tttctggctt tactgtaaaa tccaattctt ccatcgaacg aaacaatttt
    12241 gcccatcgtt catcaacaat ccgtgcatca ctttccagtc gatcccaaac aataagcttt
    12301 gcattaatta aaccaacgcc aatctctcct aaacgtttcg gtgaatcacc taaaataaat
    12361 gcttctactg cttgtgttgc tttctcattg gctagccgta attcatatcg attataaatc
    12421 ccatatttcg ttaatgcttc taattctgaa attttttctc ggttcgcaat ttcaaaacgt
    12481 ttttcataga aattgaaaaa caatggactc tttcgcgatc caaaatacaa ggataatcct
    12541 tcacttcgaa aatagccatc atctaaaaaa caaccgccaa tattctggaa tctcttaaaa
    12601 tccatttcta atagacccgc tttttgtttt tctagtaatt caaagagatc gaaatgttct
    12661 tccgtttcca atccccaaag ctcatctaac gcaatatcga atcgactaca ttgcacacgt
    12721 gtataaccag cttccaactt tccttgattt ttccaaatag aatatagcca tgttcgccaa
    12781 tctgtttgtt gttcacgcat gacttcctca tattcccggc aaccttgtcc tgaaaattgt
    12841 acaagtacat tttctgttac ggttcgtttt gaatgccaga acacccagat attcccacga
    12901 acagaaagac agtcaaaaag atacaagccc gttggacgtg tttcaaattc tgtcagatcc
    12961 attcgtaata cacgttgaat cactgttttg tagtctgtcc caaaaaagga aagacgcaaa
    13021 taatcaatca ttgctcgaag atctattccg ctttccacat acaatcgaaa gcgttccttg
    13081 attcgttgtt caaaatcctc tgggattttc ctttttccct gttccatctt ctttatataa
    13141 tctactgagc atcccaataa ctgtgcaaaa cgtttttgag agagtaactt cttccctcca
    13201 cctgaagcgt gaatagcact caattttttt cgataatttc gcaattcaat tggcgtcata
    13261 ttctacctcc tttataaaat aacaaatttc gattttttca atctgctaga aatgccggta
    13321 tcataggtgg taccccctga tttttctaaa aaggggtact tcttctaact ttagctaaag
    13381 ccttgatata actgctttaa atatactttt ttatttgtgt tatgttattt ctgcccccca
    13441 tattatatat cggggggctt tacacaaaga ggccgtgcgc aagcgcacga cctctttgtg
    13501 agctgcacac gctgctagcg agtgcgcatt ataggcccat gcttcgcagc cttaatgcgc
    13561 accgccaggc agagtgcggc taccagacta cgtctagagc cctcccgata gataataact
    13621 acaaaattaa atgatctaat tttgttattt cactatattt ttcaggagaa aaaacgcgat
    13681 aaattgacaa agttaaatcc tcttctaaac ctactcgata atgatacaat cgagtcaatc
    13741 gttctatatc ttttttcgtt agcaatactt gattaataac ttttcctgta aaaacacgtt
    13801 ctttgcgcaa aaagcggtaa tcagttactt gttcaatacg attcaaccaa tcataaaacg
    13861 ttctggaaga aagagaagga ttaatcattc gtattgctgt agctactcga taataacaac
    13921 tctctgccat ttaacttcac ctccttagtt gattacaaaa tatgcgcatc agaaaaactt
    13981 gttccttttg gatcttttgc taaatacatt tggcgctcta gttgttcttg taacaactga
    14041 ttatcatctc ccatcgtttg atcttgacta tcctctttta ctgtaaaatc atagacaatc
    14101 atttcagtaa atttctcaaa ttctttgata aagtgatagt ctttgtaaac aaatggcgca
    14161 aagaactcta caggtacatc aaatcctgga aaagcatacc cacgaccagg ttgatctttg
    14221 ttgtaaaatg gcttattttt aaaatcttca ccaaaagtca ttaaatagcc aatcggagat
    14281 aatcgaccaa cagaaatacg gaacgttaaa ttatctcgaa caccacttgg aaaatattcc
    14341 gcatctggtc gttgcatcgc taaaataacg tacatgcctg cttgacgacc ttttaatact
    14401 aatggctgaa tcaatttatc gactttattt cttgtttcca tatccaacga accataaaaa
    14461 gctgcccatt catcaaaaaa gataaaatgc ggcgatatcc cataataagc ataatttttc
    14521 cctggttcat acttaggttc tttcatcgtt ttcatatagg catatcgttg attcatttct
    14581 tttaacgcat ttcgtaagca agcaatcatt tcttcattcg ttttgtaaaa cacatgtcct
    14641 ttgaatatat caacgtcacc taaagccgct aaatctgccg cttttccatc gcaaatatct
    14701 actgtcccaa catctaaaca agctttgatc aatgaataca ataaataggt ttttccgcca
    14761 ccaatcgcac cagaaattaa cagatttggc gattgatcat atacccaatc caaggttttc
    14821 attaaatgaa tacgtccatt ttctgctata cattgatcaa taggaatccg accagaatcg
    14881 gaaagaaata cataattgat tcgggctttt tctctttctt cacgaattaa atcacgcaaa
    14941 aatacttcag ataacaaatt acccattttt aaaaatcgtt cgtggaaacg cccaccatct
    15001 aaaggaaaag aaatcactaa ttcttgtttc ttttgataaa aataaacttt tggtaatctc
    15061 attttttcct tcatttgttt ctcaccaact tgcttctttg tataaataaa ccgattatca
    15121 attaacattc gagcaagtaa ttgccgccaa taaacagagg caaaatatcc ttcttttaat
    15181 cgcgaccaac gcatcaaacc taaaattaat ccactaacca gcaataaata caacacaata
    15241 cttattccag gaataagata catttgccaa ttcgaatacg tacttaaacg cggataaaat
    15301 actttccaac taaaccaacc taacagaatc aaaaatggga gtaaaaagaa accataataa
    15361 cagaaccaca aataacgatt gaaataacga attcgccgac cacgatactt aaaaaaatga
    15421 ggttccaatt gtcacattcc tcctttctgt ttatacttct cctgtgctaa aaactttcga
    15481 caaaaagcaa ctaatactgg aatatcacat ttaataaaaa accataattg aaaacgatct
    15541 aatcgatttt tttatgggtg tgcacacata ttacatattc gtactacacg aatccaaacc
    15601 tcttcctgat gattttgttt ccactgatat ggtaatgaaa aacgaacaag atcacgtaaa
    15661 aacatacact gcccaataac cgcacaaata tatgcatttg tttgtgcaaa aatcgtcgga
    15721 ttgtctccaa aatactcaat caattgttgg gtattttgac aacaagccac aatctctcga
    15781 agtgtttgat tggttttata tgaattcaaa atctcccctt ccttctacct tagataaaaa
    15841 gaataggaag acttcttcct actctttcaa aaaatcctta ttttttctct ttttccttag
    15901 aatcaacact tggttgtttt tgatctaaac ctttttcttg tgattgtttc agattaggat
    15961 tatttgttgg agcttctact ttctctaatc ctttcgcata aatcactaaa tctgaatatt
    16021 cacgattgcc atcttgtgct ggaatatata ccacatgata ttctggatca attaatttca
    16081 caggatcacg tcgtttaaaa ccagtcaaat ctacatctgc tggaaattta actcgtcgtg
    16141 caatctttgg ttcatctgga tttccaaaaa ctaattgtgc tcgctgtgat cgaatttctc
    16201 ccgtataatc gcctgtttca ttatcaaata cacgatcaga ctcttctaat gcgccaaagc
    16261 gtacggctcc tacacaagtt tcatctaatg cttctgtaat atcataatta tttggaaatt
    16321 ttaatcccat cttacaaaac ctcctcaatt tttacgccag ttaaaaagac accaacatct
    16381 tgatagccag ttgttcgacg ctctgtttgt cccatatcat ttgtttcacg actattcact
    16441 cgttctttac gaaaaacaaa ataagcttct tcaaatgaaa tacgcacctc tttttctggt
    16501 aatcgtaatc ttttatcctc ttcagctgtg agataaacaa aaaaagcagg ctgttcttct
    16561 cgcgaacatt ctgcttgtaa ttgaacatat cggccagaca caccattaat ttcctttggt
    16621 ggattaggtt cccgtgaacc caataaaaat acttgaccta aaaactcttt cttcatacgc
    16681 aatgcttttt ctgccataaa gtttttcctc ctcttaaaaa ccaaaataaa ttctaaatat
    16741 ttctacatta caatcaaaca agcggttaaa aatcctaacc gcttgttcct catgcctaat
    16801 ctttggcttt acgtacttta taaagtgtta tacctaaaac aatgactaat actactaatc
    16861 caagaaccat aagtatacta cttgtttgtt cgcctgtttt tggcaacatt ggtacaacag
    16921 tagctgcttt aaccggttgt tctggtttca ctggttgcgg tggttttggt gcctccacaa
    16981 attgaaccgt ttgatctttg tttgttaaat ccacgttatg ttttacgact tcctcaaacg
    17041 cttctttgtc atcttctgtt ttggcttgaa gccattccac aaaacttaag ccttttcctg
    17101 caaggttgcg accgtccact aagaattcaa ataagaattc catcatttct ttatcagccg
    17161 taaagacttt ttctgcatgt gtaccttcaa atggcgtgcc gtctggtgtt aagtagcctt
    17221 gaacaagcag tttgtacaac tgaccaatcg caagaccgtc tactttaacc cattccaaca
    17281 cggtttgatt tggtaaagcc aagatctctt ttccaccatt tttattggta gctttggttg
    17341 taatggttgg attgacgatt ttgatggttt gatctttgtc ggtaatatcc gcatgagaag
    17401 ctaataatac accgtctctg aagagatctt caaagaccac gacttctttt cctcgtaacg
    17461 cacgtgcgtc caacacaaag tctagattga ctgtaccgtc gatacgttct gccacaaacg
    17521 ttaacgtact agtaatttct ttgtcgtcta ccaacactgg tttttccgtt gatttgtcca
    17581 ttagaatacc gtttactgta tatgttttcc caacgattaa gtcgtggtat ttcacagcgt
    17641 ctgtaatggt gattttcatt aatggattaa ccactttcaa gccagttggt ttatgcgtag
    17701 ccgttgtacg tacacttggg ttcgttactc gtaccgtttg ccctttgtcg ttgatgtctt
    17761 tgtggtttgc cagttcttca tcatttctaa aaagactttc aaatacgacg atttcacgac
    17821 catttaaacc tgtcaaatca aaggtaaatg gtacgatcac cgtttcattt tctgctttgg
    17881 caataaagcc accagcactt tctactggtt gaccatgaac aagtaaggct tcaccagttg
    17941 ctttatccat taagatccct ttgaccaagt aagcttttcc tggaatgatt tctttgatct
    18001 taaccatatc atttaaggta aacagccccg ttgggtctgc gaccgtcaat ccatcttcaa
    18061 atacagcggt tgtatgaaca tctggattgt ttactcgaac actttgattt tcatcattga
    18121 tattcgcatg cgccgcaact tcaatgccat cacggaaaag tttttcaaag acaacgatcg
    18181 tttgcccttt taagacgctg gcattgacca caaattcaag gtcttttgtg ccattgactt
    18241 tgtctgcaat aaaggtcaaa gtttttctga tttctttacc atcttgtaag atcggtgtat
    18301 tcgttgcctt attcatcaat actcctgaaa tggtgtattc ttttccgaca aacaggttgg
    18361 tataagaaac actgtcttta atgacgatcg tttctaatgg atcaaccact ttttcaccat
    18421 cggctttatt tgacgcttgt gtttctagtt ttggcggaat caagtagtta cgtacagaaa
    18481 ccaataacgt ttcgccatca tctggcgcat tttgtttgaa tgtaatagtg aatgcttgtt
    18541 tgtctgtatt tggttggaaa ctttctaatg gtgtattttc gataaagtaa tagttattgg
    18601 caactagacc tttcactaaa atctcaccat tttgatcggt gatatattca ccaagagctt
    18661 tccctgcttg gttatacaga gtaaaggtga caccagcaag cggtgttttc ttcttgttcc
    18721 attcttcata cttgaacaat ttcacgtttt tccgtgcttg acggttcgca aaatggatca
    18781 ctttgattgg ttctgtatca ttggtaatcg agaataccaa tggttttgat tcaaaacgta
    18841 atttacgggc gctttaattt cttccaaacg atagcgatct tttccataag taaatgtacc
    18901 gttagttaga aaaaagcctt tttcattggt tttaaagata tccgttgttt ctgtatcatt
    18961 tggaatctcc attgtgacat aacgattggc taaggtatca aaaatcttga attctgtatt
    19021 ggcaagtgga atcacttttc ctgtttcttg atccactttc accactttaa gacgttgttc
    19081 gattacttta tcttctaaag cataatggaa agtttgccca ttttcagaaa ttgtgacttc
    19141 aaatggttca aatagtttat acccttcttt tcctttggtt tcagagagaa tatacgtatc
    19201 aaaaacaacg gcatttttac caaaagtagc atagccatct ttatctgtca caactgtttt
    19261 taccactttt ccagttgttt ttgacgtcaa agagatttca ataccagcta attttggttt
    19321 attatctgga ttcattgaat cgattaagtc tttattagca actttcacta aatcaatggt
    19381 gcccttaatc acttggtctt ttgcggtcgc attgacagtt tttccaggtt caacaacaat
    19441 ttcaattgct tcatcagaaa gcacataacc ttttggcgct ttgatttctt ttaaaacata
    19501 tttgccagga tcaaggtctt taatttctgc ttgaactttc ccattgacgt tcttcaaagt
    19561 aacttctttg attttcttcc catcggcttg atacaaacca taaacggcgt tgttcaaaga
    19621 agaggcacct tgtgctttca cactagtttc tgcatcttct ttaaagacac gaatgtttcc
    19681 ttgtttcaaa atagtcaaat tgacaacaga gtcaccaggt tcaaaaggat tcaatatacc
    19741 aattgtttgg gcacctggac gacgatacgc taatggcgta cccaatactt caatctgatt
    19801 gaaatgaatt tttgcctttt ctggtgcttc ttttgtgact gtgatccatg cttcattccc
    19861 atgaatagat acattgatac cttttggctt ttgaatgcct tttacacgtt gtaaaaagtc
    19921 atttccactt tttactttta acgtttcgcc aatttttatc gtatgtgttt gattatgaaa
    19981 cgtcgctctt acatcaaaat tattaagcag acgttgaatt tctgcttttc gacgatcata
    20041 atcaacacgg atatttttga ttgggatttt tgtgcccaaa gcttcccaaa tcataaattg
    20101 tgccgcaatg tattgttcat cggatttgtc tttatttgtg acatatccta aataagagat
    20161 caagcttgtt tttttccgta catttgaatt tttcaaatag ttttccaaag cagtagctgt
    20221 ataaacttga ccttttgcgg ctgagacacc aggctcaata caaaacgcca cttgtccatt
    20281 aatctcaaaa cgaaccatat aatttaaagt gaagtttgga ttgttcggac tagaagtaac
    20341 accaatattt tcgccccaat caattttccg accacctaaa acagctgtat tggttgcacg
    20401 gcgaacacga accgaacgat tggttggtac tgttactaac agtgtaggtg tacctgtttt
    20461 tggaatgtcc tgctcttttt tcttttgatc ttcaatacta gccgtagatt ctttgtttgg
    20521 tttcgttggt tgattttgac tagctgcggt tgaagagatt gttttttcaa cgttttcctc
    20581 attttttcct tctttgtcag cagtagccgc tggttgttcc actgtttctt ctattcgttg
    20641 cttttcttcc gcttgtttct cagaagactc tggcttcaca gaaggcgttg cttcggcagc
    20701 tttttctcct tctggagtgg acgtttctga agcaggggtt gtaattgagg cagtttcttc
    20761 aactggttgt gaagtaacag gactattccc tgggtgttgt gtttccccaa tcgtctgatc
    20821 gagagtcgtt ttggaattga ccgttggcgc ttctgtcaca ggggcctgtt caacagtggc
    20881 ttcaccagct gggaccccgg cttctgctgg ttggacttct gtcacttcag aagcgacttg
    20941 ggcaatggct tccaccgtag gttgtgtagc cgtcgtttct tctgcatgtg cttgagcagt
    21001 tcccaccaag aaacagaatt gagaaaaggc caccaatgcg agtgctttca accaattcat
    21061 tttcgttttt gcatacacaa gaattcctcc ttttcctgag aattttatat aataaaacaa
    21121 aacgccgtta ccaaaagtga taacgacgtg aactgtctct tctattttta tctggcgctc
    21181 ttatctgaac acatttcatc aatgatttta ccctcccact ataattacag aacaaaaaag
    21241 aacctgcttc gtggaaatca actgtccatt tcccaattgg aataccagct ttattggcaa
    21301 cttcaccatc accaaatctc atgccatacc aattactacc ttcatcctta atttctttcc
    21361 aaccatatga catagcttca tctaacgaat caaaaatcat gccactgttg ccaattgttg
    21421 caggatctgg cttaagatct tctggaagag aaatatcaat cacaccataa tgatccgatt
    21481 cctcagtatt attgtcatta ctagctattg gtttggttgt tctactggct tattttgata
    21541 caacaagtac gcttattctt cttttacttc tccgttttca atttcccatt caaaccactc
    21601 agcaaagtct tgcttaaaac catctactcg atacctattt tgtaaatagg catcttctga
    21661 cgtcgtagga tctaaatagg taggtttatt ctctggacga gcatagacac ctacccatgt
    21721 ttcgtaatct ccttcaaaat aactatctac caaatagcct ttttttctaa gaatctcccg
    21781 aacttgattt ttaattattg atacttgttc aaaaccatca ctcattctat cacctcaaat
    21841 aactctagat tctcataaat attcccgaca atttcaatcc tatcaatgat ctctaacaaa
    21901 tcgctactta tgcttcccca ttcgtataag aatttccctt ccgcaaaata aattttccat
    21961 aaacttcttc atgatcgtcc caaccaatat cactctcgaa aaattccacg ttgttcttat
    22021 cttttagctc agtaaatgcc ataagttcaa catgttttcc aacctcgaaa caatcacacc
    22081 acccatcaac cgttacacgt gtgccttcaa tacctaagaa actttgtcct cgtgtattaa
    22141 acactaaaac tttccgcatt ttttgctcat taattaatca cgcgcgaaat ctaggaatca
    22201 caacttattc gcttcttctt tataaataaa aaaactccaa taatcggaaa ttactatctt
    22261 ttcctcattc cttttttaaa tcttcatact tcttcttatc tttgtcctaa cctaa
//
LOCUS       DADXTG010000039        21038 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.039, whole genome shotgun sequence.
ACCESSION   DADXTG010000039 DADXTG010000000
VERSION     DADXTG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.039"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..795
                     /locus_tag="IWI57_001230"
     CDS             1..795
                     /locus_tag="IWI57_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBC2449386.1"
                     /translation="MWLAGCSQGKADTTDKKETIVKLGIIGEDTDVWDNVKDRLKKEN
                     INLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGTDLVSIGNTVNAPLGI
                     YSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDKAKGQAPTVSDITENR
                     KNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIFLEPVDDHARPYVNII
                     VARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETFGRK"
     gene            826..2049
                     /locus_tag="IWI57_001231"
     CDS             826..2049
                     /locus_tag="IWI57_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBC2449387.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            2452..3870
                     /gene="nhaC"
                     /locus_tag="IWI57_001232"
     CDS             2452..3870
                     /gene="nhaC"
                     /locus_tag="IWI57_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBC2449388.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4374..4817)
                     /locus_tag="IWI57_001233"
     CDS             complement(4374..4817)
                     /locus_tag="IWI57_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBC2449389.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(5035..6375)
                     /locus_tag="IWI57_001234"
     CDS             complement(5035..6375)
                     /locus_tag="IWI57_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2449390.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            6644..7540
                     /locus_tag="IWI57_001235"
     CDS             6644..7540
                     /locus_tag="IWI57_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBC2449391.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(7690..8631)
                     /locus_tag="IWI57_001236"
     CDS             complement(7690..8631)
                     /locus_tag="IWI57_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBC2449392.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            8757..9878
                     /locus_tag="IWI57_001237"
     CDS             8757..9878
                     /locus_tag="IWI57_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBC2449393.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            10136..11638
                     /gene="eat(A)"
                     /locus_tag="IWI57_001238"
     CDS             10136..11638
                     /gene="eat(A)"
                     /locus_tag="IWI57_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBC2449394.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            11666..12232
                     /locus_tag="IWI57_001239"
     CDS             11666..12232
                     /locus_tag="IWI57_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBC2449395.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            12443..13504
                     /locus_tag="IWI57_001240"
     CDS             12443..13504
                     /locus_tag="IWI57_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBC2449396.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            13507..14403
                     /gene="murQ"
                     /locus_tag="IWI57_001241"
     CDS             13507..14403
                     /gene="murQ"
                     /locus_tag="IWI57_001241"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBC2449397.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            14417..16399
                     /locus_tag="IWI57_001242"
     CDS             14417..16399
                     /locus_tag="IWI57_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2449398.1"
                     /translation="MAKNELTLEELAKHIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(16464..16910)
                     /gene="nrdI"
                     /locus_tag="IWI57_001243"
     CDS             complement(16464..16910)
                     /gene="nrdI"
                     /locus_tag="IWI57_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBC2449399.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            17121..18545
                     /locus_tag="IWI57_001244"
     CDS             17121..18545
                     /locus_tag="IWI57_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2449400.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(18726..19499)
                     /locus_tag="IWI57_001245"
     CDS             complement(18726..19499)
                     /locus_tag="IWI57_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBC2449401.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(19496..20401)
                     /locus_tag="IWI57_001246"
     CDS             complement(19496..20401)
                     /locus_tag="IWI57_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2449402.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(20555..20875)
                     /locus_tag="IWI57_001247"
     CDS             complement(20555..20875)
                     /locus_tag="IWI57_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBC2449403.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         6782 a         3823 c         4351 g         6082 t
ORIGIN      
        1 atttggttgg cagggtgtag ccaaggaaaa gcagatacaa ctgacaaaaa agaaactatc
       61 gtaaaactag ggattattgg agaagataca gatgtatggg acaacgtgaa agaccgtttg
      121 aaaaaggaga acattaattt ggaatacgtg aaatttacag attacaatca accaaatgtg
      181 gctttagcag atggatcgat tgatttgaat tctttccaac atcaattctt tttagacaat
      241 tacaataaag aacacggaac tgatttagta tcgatcggaa atactgtcaa tgcaccgctg
      301 ggaatttatt ctaacacttt gaaggatgta gctgatatca aagacggagc aaaagtagca
      361 atccctaatg atgttacaaa tggcggcaga gctcttctgt tattgcaaac tgctggattg
      421 atcaaagtgg ataaggcaaa aggacaagcg cccactgttt ctgatattac cgagaataga
      481 aaaaatctag acatttctga attagatgct tcccaaacag cacgtgcttt gtcagatgta
      541 gacatttcag tgatcaatag cggtgtagct gtagatgctg gatttaatcc tgcttctgat
      601 gccatcttct tagaaccagt ggatgaccat gcacgtcctt atgtgaatat catcgttgca
      661 agaaaagaag atgaaaacaa taaaacgtat caaaaaatag tagacgctta tcaaacagaa
      721 gacacagtca aagtaatcga gaaaacatca aaaggatcaa gcgtgcctgc atgggaaaca
      781 ttcgggagaa aataagaaaa atgactagca taggaggaat tttttatgac aacaacaatc
      841 tcaacaaacc aattgaagga tctaatccaa tatcgtttac cagaatatca agcacttgct
      901 ctagatatcc acgaacatcc tgaagtaagt aactatgagt tttattcctc agcagcgttg
      961 attaaacaat tagaaaaaga aaggttttgg gtagaaaaag aagtagctgg acaccgaaca
     1021 gcctttgatg cacgttatat ttccaataaa cccggtccca caattgcctt tttggcagaa
     1081 ttcgatgctt taccaggtat tggtcatgca tgcgggcata atttgtttgg tacttactcc
     1141 gtattagccg ccagcatagt gaagcaattc attgatgaaa ctggtggaga aatccgtgta
     1201 tatggcaccc caggggaaga agggggcgag aacggatcag ctaaaggcag ctttgttcga
     1261 gaaggttttt ttgaggatgt cgatgtagcg ttatgcgtcc atccagcttt tcgttatggg
     1321 aaaacgacag aatctttagc gaatgatcca gtcgacatca aattttacgg tgtcgcttcc
     1381 catgcagcag cagcacctga aaaaggagta aatgctttgg aagcattgat ccaagtgttt
     1441 aatggaatca acgctctacg cttgcaattg ccaaaagatg tcaatatcca tgggatcatt
     1501 acagacggag gagtagctgc caacgtcatt ccagattacg ctgctggacg tttctattta
     1561 cgtgcaggaa atcggcaaac attagataaa gtatatgaaa aagtggaaaa tatcgtacga
     1621 ggtgctgcga taagtacggg cacgactttt gagtttggat tgttccaaaa tgcagttgat
     1681 gatgtcatcg ttactccttc ttttgatgac ttattcttcc agcatgcaaa agaagcaggt
     1741 gttccagatg aagaaataga aacggaacaa agaacaagtc tcggttcttc tgatgtagga
     1801 aatgtcagcc aagtcatacc aacaatccag cctacggtag caatctctga gacatatatt
     1861 gccggacaca cagaagaatt caaagctgct gctaaaagtg acaaaggcct tgcttctatt
     1921 gcgattgcag cagaactact cgccaataca gctcttgatc tgttgaaaga gccaaagcta
     1981 ttagaaagaa tcaagcaaga acatcaagaa atagtagcaa gaaaaaaaca agagggcgct
     2041 actttctagt aaaaaataaa cttgtgtgtg aaagttataa aaataatttt cagaaaaatg
     2101 aaaaaatatt ttctttttca ttgacaaatg atcttatgga aggtaaactt cgtacaagtc
     2161 aacttttcac gcagtagcgg ttaggtgttt tgttcagaaa gtcggtggtc gctgtgaacc
     2221 gatcaagcct aattgacgaa ttacatggaa agtccataca tttgaataaa agtttgaaag
     2281 tgaaaaacag atctgttttt aattaaggtg gtaccgcgga gtcattcgtc cttagtttaa
     2341 aacaggtctt tttattttta taaaggagga atcgcggatg aattgcggcg gagtaatccg
     2401 atatcttaac ttttttagaa cataagaaca actaaaaaag gagagattat catgggaaaa
     2461 aaaggaacac caagtttttt agaagcaaca cttgtattaa tggttattgt tttaggcatt
     2521 gcgattggcg ttgtcggatt aaaggtctca cctaatatca cgatcttagc agcgatcgga
     2581 atggtcatgt tgtatgcagt gataaaaaga tacccgacag attggctgca cgaaggaatc
     2641 atcaatggca tcaaaccagg aatcatccca atctttattt ttattttagt cggttcattg
     2701 attgctgtat ggatacaagc agggatcatt ccaactctaa tggtcattgg gttcaaattg
     2761 atcagtgtcc agtggtttgt cccttctgta tttgttgtgt gtgcaatcgt aggtagcgct
     2821 gtagggagtg cctttactgt tatgtcaacg attgggattg catttttcgg tattgggaca
     2881 acactaggct taaatccagc gctggttgtt ggagctattg tttcaggtgc cgtttttgga
     2941 gataagatgt ctccattatc cgaatcaaca aatctagcag cagctattgt tgatgccgat
     3001 ttgttcaaac acatcaagca tatgatgtgg tcaactgtcc cggcatttgt tgtttctttc
     3061 tttttattca tgattttagg acacacaagt aaagccgcaa gtctttcatc tattcgtgaa
     3121 gtgactgatg ttctagaagc aaactttact atttcaatct ggtcactgat tcctttattt
     3181 ttaatgttac tgtgtgcatg gaaaaaggtg ccggcaattt taacgatttt gttgaatatc
     3241 gctgtagcag taggaatgat ttttattcaa aacccacaaa cttccttaac gaagttggct
     3301 ggggtcatcg aatcagggtt tgtttcaaat actggaaacc aacaaatcga ttcactgctt
     3361 agccgcggag gaatcgaaag tatgatgcct actgtgtcat tgattattct aactttgtca
     3421 ttaggtggat tgttgatcga atttggcttg atctcaaccg tgatggaacg agtatcaaga
     3481 aagatgacga acacaccaaa tttgattttt acaacattga tgacaagtat cggagtaaat
     3541 atttttattg gtgaacaatt cctatctgtt attctgcctg gtaatgcatt taaagaaact
     3601 tatgaaaaag caggatttga tccaactgta ttaggtagaa cgctagaaga tggtggaaca
     3661 gtaatcaact acttagtgcc ttggggaatt gccggaagtt tcgtagcaag tacatttggt
     3721 attcctacac tcacttactt gccattcgta tttttcagtt tgctttctcc tgtgttttcg
     3781 atggtcagtg ccttcacagg ttggggaatc tcaaaaaaat ttgtgaaacc gcaagaagca
     3841 gtaagcaaga cagcgcaatt agaacaataa aaacataaaa agggattgtg acaagacttt
     3901 tgtcacaatc ccttttacga ataaacggta ttcaaaagcc agtttctcgg tattaagcca
     3961 aatttataca aaaatgtgga aagccatttc acaaaatttt tcttaatacg cgaaactaag
     4021 cgactttttc acaacctctc gataaacggt gttcaatcag ctgaccctcg gtattagctc
     4081 aaaaaatggc aaaatatgag gaactatttt tccatttgtc tctctaatac tcaggtcaga
     4141 acgctgattt tacaacctct taataaacgg tgttcaaaag cttgcatctg cggtaataag
     4201 cctaaccaaa cgaaaaatag gagaagctat ttttgtttgg ctgttcttat tacttgatgc
     4261 agggcagctt tttcacaacc ccttattcac ggcgttccag aagtagcttc ttcttatttc
     4321 ttgaagctga taacttctgg aacatcctct ttttttattt ggacggataa tggtcattcc
     4381 atttctttga tcattttttt cattttgtcg atctcttctt cttgtgcctg aatgatttct
     4441 tccgctaatt tttttacttc aggatctttt aaatcagctc gtttacttgc caggatagcg
     4501 atagaatgat gaggaatcat ggccttcatc caagctgtgt cgttgacagt agtttggctt
     4561 cgtaccataa acaaggaacc ggcaaaaaca agaagactag cggctaagat cataacattt
     4621 atctttttgt tttcatacat tttccacatg aacaacagca tgatgactgc catgactgca
     4681 cccatcatca acgccatgaa tactcgtgta cgactgaaga agatatgatc gagcgcatag
     4741 acattcaagt acatcatacc aaacataact accgttgaaa caccgatcat caacaaaaac
     4801 ttccaatact ttttcattta aaacaaactc ctttttttca ttcctttctt ctatcatgtc
     4861 atgagcatgg agaacaatca attattttgc acagagggtt tttgaacacc gtttaccaag
     4921 aggtcatgag actgtttatt cggaaaaagg agttgcaaca agacttttgt cacaactcct
     4981 ttcttcaata gctccatcta ttccacataa ataaaataaa attttctatc gatttcattc
     5041 attcgtcaat acgaacgcat cattcttttt ttgataactg aaataaatga acaggtaaag
     5101 caggccagtc aatacagtta ccccaatgga gttcgtgaac aggataccaa ggaaaatcaa
     5161 tcctaagaca gctaatgcaa aaaagctgat tcctttatcc cataatgttc cttttcttag
     5221 tgccagtaca aaggatgccg cactgataat gatccagatc aataatactg tatacgaaag
     5281 ggaacctgcc agatagccaa ataatttgtc accgacaaaa taagaaagag ctacaccaag
     5341 ataaagaaca ctggtacaaa ataaaacagc acgttgcggt acttgatgct tgtttaaaac
     5401 agctagtttt ctagaggaac cttttcgatc cttcaaacgg aagaacaaaa ttcttgaaga
     5461 tgcatacaca cccgaattga tagatgagaa caaagcaagg acaatcacaa aattgacgat
     5521 atccgctgca aatgggatat tcattttttc aaataccatc acaaaaggac tgacattcgt
     5581 gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa aacaaaggaa tgatgtaaaa
     5641 ggaaatgatt cttcccatga cacctttgat tgctttagga atcgcacgtt taggatcatc
     5701 tgcttcgctt accgtgatag caatcagttc agaaccgccg taagaataga taactacaag
     5761 caaagaactg atgattccct tcataccatg aggagtaaac ccaccatggt cagtcaattg
     5821 ctgaagggta gggaaaacgc ctgtgcccaa catttgctga ccgacaagat aagtagcaaa
     5881 aatgatcagt aagataatga cactgatttt agcaaaagcc agccaatatt ctgtttctgc
     5941 gaataaccgt accgaataaa gattgatgat cgtagttaaa acagccaaca taaaaacaaa
     6001 tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag gaagcagctg caacagcttc
     6061 agcgatgata ttgataatcc acatagacca gtagacccaa tcagtaaaat cagcagaatg
     6121 agcacctaga taagggcgta tcaaaccaga taatccgtga tgcgtgtcgc caccgcttaa
     6181 aacgattttt cccagacctt tcatcacgat atataaaatc agcccaccaa tcaaaaaagc
     6241 aatcaataca gaagggccgg cgatcccaat cgcttcgcca cttcctttaa atagacctgc
     6301 accgattgct ccacctaaag ctaacatggt gatatgtctg gagctaagtt gttttttcaa
     6361 ttttttatct tccataaatg acctctttct tttaaattca caatcaaaat ttatattaag
     6421 aataaatagc aatatttaac aatgactaat gataacaaat tttcagacaa ttacaactat
     6481 tcagaaaact tttaggcgaa ttttaataga agaatgattt atttcatcat attttcatac
     6541 aaatgttaaa ttttcataca aatataggaa acgttttact ggctttttac tatttaaaca
     6601 ggctatcata ggaaaaagtg acagagaaag tgaggcggaa aagatgaaca aaaatgacgt
     6661 actgctagtt gcaaaaaata aatcacttta ttttttgcct tccatccgct ctttagatgt
     6721 ttggtccgga aaagatttac tacagtcgga attcattaaa tcagctagac aacagttggc
     6781 aaattatcag ctgatcgttt ttttagatta tggatttgat atggagatgg ctcgacgcgt
     6841 gcgcccattt accaatgcaa aaattgtgtt atttttttgg aatcacttca aagcagaaca
     6901 cgaggcgatg ctgaagcagg cacaagatga accggcaatc gatgagattt atcattttga
     6961 ttttttggaa gcaaaagaac ttgggctgaa acataatagt tcattttatt cgaaacatat
     7021 gaatttttct tctagcgaac caaagacgga tctatttttt ggtgcaacga ataatgggag
     7081 aaaagaacga gcagaaagct ataagcagga atttataaaa agagatgtta cgaccaacta
     7141 tttcattctt ccacatcgag ggaatgaaca acctggatat cttacttatg aagagtacct
     7201 gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg cgggagggtc agcaaggagt
     7261 gactttgcgg acatttgaat ccatgtattt tcaaaagaaa ctagtgacgg ataatcaagc
     7321 gattgctcat tatcattttt atcgtcctga aaatattttt ttgcttcgag aacgtgattt
     7381 agatgaatta ccggaatttc tgcatacacc atttaagcca atcctatcag agctattgga
     7441 attttatgat gcagaaaatt gggcaaaacg atttatcact aacgattcca ctatttatga
     7501 gcaatacgaa tatgacctct cctctgttga aaacaaataa aaaacctaca ttttgaagag
     7561 attatgtact gaaacaaaca agcacaattc ttatggcatc atgagagttg tgttttcttt
     7621 ttgatctttt attgggctta aaaaaagaga accagctgct gattcagctg attctcatca
     7681 gagagccttt tattctgcgc caaggattcc ttcttttgca tggactaatt gggtcaaaaa
     7741 ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt cgccaaatgg caccattgat
     7801 gtagtcgatt tcagtcaaac gatggttttt gatcaggtct tggtacatag atgggtaatg
     7861 caacccgatg ttgtcaggat cataagtaga agcaatatgt tggtacactt cttcttgatc
     7921 tagtatgatt ccttcttttg ctgcgataga agcaaattca gatacgattg ttcgaaccat
     7981 gctctctgcc gctttttgcg cgcctagttc agcaatatta caatccaaaa tcgtgcataa
     8041 accattcaat gttccattta cacaagcttt tcgccaaatg gaataacgaa cgttatggct
     8101 atagatcgga tttaaatttg cttcttgaaa gacttctaca actttttttg taaatgcttc
     8161 gccttctggt tccaagttct ctagttcgat ttctccatca cctaacaatg tgacttttcc
     8221 gggaccagct agaccagctg tccacatcgt gatcccaaat aagatatttt tctttggcac
     8281 atatttttcc agtacatctt catgacctaa tccatttagt aaacacaaaa cataagtatt
     8341 gtcagtaata atggattgga tcgaacaaaa catatcatct aattgattgg cttttgttaa
     8401 tgctacgatc aagtccacgt gttcgttgct ttcgatgatt tcttctggtg aatagattgg
     8461 cagttttgcg accacttctt taccgttaaa gtcagcaatc aacccatttg tacgaatggc
     8521 ttcaatgtga gcaggccaac gatcgatcaa gaggacctca tttccgcttt gatgtagcat
     8581 caatccgatt cggctgccca ttgctccggc cccagctatt gcgattttca tgttttttct
     8641 ctcctttatt caatgagttc ccttttagta tatagaaaaa tggaagaaaa tagcattgtt
     8701 ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag aaagtgaggg atacagatgg
     8761 atattttggc ggcaattgat tcatttaaag gaagtgcttc ttcatttgaa ctaaatcagg
     8821 cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat caatgtgccg attgcagatg
     8881 gcggagaaaa tactgcagaa gcaatctatt acgcgctcag gggaacatgg cgtaccattg
     8941 atgggatgga tctcttattc cgtcagcgaa aaatcagcta tttattgaca acttggcaag
     9001 gaaaaaacac cgcagtcatc gaatctgcac aagcattggg gttagatcta cttgataaac
     9061 caacggatca aactatccga cttgcttcca gttatgcctt aggagaactt attaaagatg
     9121 cactgcttca agaagtagaa caaattattg tcactttagg aggaagcggg tgtagcgatg
     9181 gtggtctagg attactccaa tccttgggtg cttctttaga agggtacaca gaaggaaatc
     9241 ctttattatc tactaaaaaa cttcattttg atcgaagaaa cgttcttgtt tctaaagtaa
     9301 aattgattat cgctgcagat gttaccaatc catatacagg tcaacaagga gctgcttggc
     9361 aatttggaag acaaaaagga ggaatagata cgacgctagc tttcttagat aaacaagcag
     9421 gaaagattgc tcagcaaata aaagatttct gggaggtgga tttagatgat attccaggaa
     9481 gtggagcagc aggcggtatt ggtggagccc tttttttgtt gggagcgaag atggtttctg
     9541 gtttcgattt agtcagccaa atcactggat tggaaaaaaa gatcgaacaa gcagatgtca
     9601 tcattacagg agaagggcgg atcgatcagc aaacgatcca cggaaaagtt ccatacggtg
     9661 tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc tatttgcgga agtagagacc
     9721 aagagctaga aggactttct gattttctcc caattgtctt aagtattcat ttgggtccaa
     9781 taggtttaga agaagcaatg gaacacaggc aaacattaga aaaaacaaaa atcttaggac
     9841 agacacttag cagatttttc accatcctcc cgaagtagtt gacaaaatca ttttctttat
     9901 gctatcgtaa tttcaaatat aggacaaagt gagggatccg tgcattgaaa aattaagtcg
     9961 attgatgaaa gctgcattca gactgtacct tgtggagaca aggaagcagt gtgcagattt
    10021 tcgggattgg cttggttttt tcgatgtatg ggtctttttg tgtgtatcca acccttgcat
    10081 cctctttagc cagttctgaa aataggactg gtcttttttt acgaggtgat ggaaaatgtc
    10141 taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc caaggcacat tattatttga
    10201 acaagcaaat ctaaattttg acacacaatg gaaactagga cttatcggac gaaacggtcg
    10261 aggaaagaca actttactga atattctaca aaacaaacta ccttatcaag ggcaagtaat
    10321 ccatcagcaa gaatttgcct atttcccgca acagacaaaa gataaagaac gtttaaccta
    10381 ttacgtgtta aatgatatta cggattttga gatatgggaa atcgaaagag agctccaatt
    10441 gatgcaaaca gatcctgaaa tcttatggag agaattcagc acactatcgg ggggagagaa
    10501 gacaaaagtc ctactggcac ttttatttgt ggatgacact catttcccgt taatcgatga
    10561 accaacgaat catttggata tctctggtag aaaacaagta gcggcttatt tgaaaaagaa
    10621 aaaacaaggc ttcatcgtgg tcagccatga ccggggattt atcgatgaag tagtggacca
    10681 tgttttagca atcgaaaaaa gtcaactgga actttatcaa gggaatttct ctatctatga
    10741 agaacagaaa aaacttcgtg atgaatttga aatggctcaa aatgaaaaat tgaaaaaaga
    10801 agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa tggtctcgtt cccgagaagg
    10861 agataaaaca aagaaacaag tcggattcat cgatactgaa tctagacgag tgaataaagg
    10921 agcagtgggt gctgatgctg cacggacgat gaaacgatcc aaagcaatcg tgaatcggat
    10981 ggagacccag atcagcgaga aagaaaaact attaaaagat atcgaatata tcgattcttt
    11041 gacgatgaat agccaagcgt ctcaccataa gcgactttta agcgtagaag atcttcaatt
    11101 agggtatgaa aatctgttat tcgagccaat tcattttaca atcgagcctc atcagcgggt
    11161 ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt atccattatc ttctgggggc
    11221 attcaacggc aaggttatag gagaaaaata ccagccaaaa catctgagca ttagttatgc
    11281 aagccaaaat tatgaagaca atcgaggaac gttggcggaa tttgcagaga aaaaccaagt
    11341 agactaccaa gcatttttga acaacctccg aaagcttggg atggaaagag atgtttttca
    11401 taacaagatc gagcagatga gtatgggcca acggaaaaaa gtggaattgg ctaaatcttt
    11461 atcacagcca gctgaactat atacatggga tgaaccattg aattatttgg atgtcttcaa
    11521 tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct gccatgttac tagtggaaca
    11581 tgatcaaacc ttcctggata aagtatctac tgagattatt tctcttgaga gaatctaacc
    11641 cttgtaatct caagaacaac aaggtatggt taaggggaac tttgccaacg aaggagagaa
    11701 tcacatgctt tatgtaacga gacacggaga aacaacttgg aacgcacaag gattagtttg
    11761 cggacgggca gatgtgccgc tgactgaaaa aggacagatg caggcacaga aactagcaga
    11821 aaaggtagta gatttgcctg ttccaatcac gaaaatcatc cattcccctc ttcaacgagc
    11881 aagagataca gcacaagcag ttgctgatcg tctcagcttg ccgctgaccg ttgatgaacg
    11941 attggtagaa atggattttg gggattatga tggactgcca agcaaagatg agaactttca
    12001 aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg gagtcagtct tagatgtgta
    12061 tgcacggatc gttcctttat tgaaagaatg catcgaagac gaagaaaatg tctatctgtt
    12121 agtttgccat aatgccttga taagagtgat caatgcttat tttcatccga tgcctaatga
    12181 ggggttcttt acattcatgg tagataatac agaattggtt tcttatgagt aattatgttt
    12241 ttgtgttatt taaataaaat atttcaaggc actgcttttg cttagcagtg ccttttttag
    12301 gttatgacaa ttcttttgat aatataaact caacctttag aaatgaaatt taattatttt
    12361 aataaaagta ttgaaatttt gtttctttag atatatactc tagtcataga aatgaagacg
    12421 gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg attctttgtc
    12481 agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg gtattttcac
    12541 ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga tcgaactagg
    12601 aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag aggcattgga
    12661 acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg taacaggttt
    12721 aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc aattgaaaat
    12781 cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag aagcagaagc
    12841 tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa cagggctgga
    12901 caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc ttgtccaagg
    12961 ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc caactttgga
    13021 agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa gtaacacgga
    13081 tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat ttgcttcttt
    13141 ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat tttacgaata
    13201 tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac gaagcgtaga
    13261 tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc gtatgaaagg
    13321 gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc aattaaccaa
    13381 atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca aagaagagct
    13441 accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa aagaaggaag
    13501 ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa ccaagaaact
    13561 tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa agaagatcag
    13621 aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat tgctaacacg
    13681 attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac aagtggaaga
    13741 ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc ggaaatggtc
    13801 ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg ggccgaagat
    13861 tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa tgatatagtt
    13921 attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga ttacgcaaaa
    13981 tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat cagtaaacac
    14041 gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc tactcgattg
    14101 aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag tatgattggt
    14161 ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga aaaactaatc
    14221 gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac agctgaaaag
    14281 tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt gacaaatagt
    14341 gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa agggacctta
    14401 tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg caaaacacat
    14461 ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca tgactcgtgt
    14521 gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag cgattccagg
    14581 agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg ggaaagtgaa
    14641 caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa aactttcttc
    14701 tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa acgaacgggc
    14761 acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca aatcggtatt
    14821 aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta ctgggatcgt
    14881 ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg atgcagcaac
    14941 atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta gctacctagt
    15001 tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc ttggtggagt
    15061 tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga aaaacttatt
    15121 tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct tcgctgtttg
    15181 gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg tagatatcat
    15241 cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct tcatcatgcc
    15301 tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac ttggcgttgg
    15361 tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta tgttcggact
    15421 gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc gtacgctatt
    15481 gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg cattatggat
    15541 tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac cagtcgggat
    15601 cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac gcccatttat
    15661 tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg gaaatgtcgg
    15721 ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg acggtaaatg
    15781 gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg ctacattctt
    15841 cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa cagccacagt
    15901 aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga ctatcctctc
    15961 atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag tttttgcaca
    16021 aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct attctccagt
    16081 tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca caacgccaaa
    16141 tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg gtacgccatt
    16201 tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag cagaagctga
    16261 cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg taaccaatat
    16321 ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa aagcacctgt
    16381 gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa actgaaatct
    16441 atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg ataaattcgt
    16501 gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa gccaaatgtt
    16561 tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt tccgctaccg
    16621 actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg catcgtttcg
    16681 gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata agtaggaacg
    16741 aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat ttcttttaaa
    16801 gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc tgctagatgt
    16861 ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat ggatcatcct
    16921 ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt aatttggaaa
    16981 gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata tttctattaa
    17041 agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat aaaagcggtt
    17101 ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt ggtttaattc
    17161 cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt attatgacgg
    17221 acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta ggaacagcaa
    17281 tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat ttaaaaggcg
    17341 ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc agcggttggg
    17401 gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg atgagcgcat
    17461 taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc gtgatgagta
    17521 cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat gctagtttcg
    17581 tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt gtagtagcta
    17641 tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt atattgaatg
    17701 cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg gtgatgcttt
    17761 gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa aaacaatcag
    17821 atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac gtcttggttt
    17881 ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag gcgcagcctg
    17941 ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg atcaacggcg
    18001 gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg attgaaacaa
    18061 tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc aacaaaaatg
    18121 gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt ttactttcat
    18181 tattattcac tgacagtgct tataactttg cttacacact tggtacagca gctattttct
    18241 tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg aaagaatggg
    18301 tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg ctagtcgtag
    18361 cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat ttttacctca
    18421 aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag attttactta
    18481 taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg atgcttcacg
    18541 tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat tcaaaaacat
    18601 actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa taaacggtgt
    18661 ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc ttttttgatg
    18721 agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa caaaacgatg
    18781 taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg cttcatgatc
    18841 tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca tttgatccac
    18901 tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc aaaataaaag
    18961 acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat catcaacact
    19021 aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa caagctttgg
    19081 atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa aagaatcagt
    19141 ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata aataactaat
    19201 gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag gagatttttc
    19261 aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat catgataaag
    19321 acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc aatgatcgtc
    19381 gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag aacagataaa
    19441 acagcagttg actttttctt gatcaattta aataattctt gttttacaag caatgtcatt
    19501 tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc aaatcattcg
    19561 tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg atcacggttc
    19621 ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc aaagtatatc
    19681 cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata acgatgaaat
    19741 cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt ccttgattga
    19801 tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg gagatcaaga
    19861 aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt gttgcttgtg
    19921 gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc agtaaggcaa
    19981 gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt ttgtgaatgt
    20041 acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg ttggtttcag
    20101 agaataactt cagatgatca tagcctgtta agaatggata gatacccggg tattcgatca
    20161 atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt tcaccaatac
    20221 agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc gttgtttttc
    20281 cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta aaactgacat
    20341 cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa agacttttca
    20401 aaaatattca ctccttttct atagatagta caagcataca cctgatgagt ggagaagtcg
    20461 ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta aataaaaagg
    20521 cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta atcttgatgc
    20581 caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc agcttatcat
    20641 gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc cagataggat
    20701 aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa ctagcaatca
    20761 tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc catttcatca
    20821 tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat gccatactta
    20881 cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct tcaaagacct
    20941 aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt tttctgtagt
    21001 atagcacacc tatccagata tcggcaatct catacttt
//
LOCUS       DADXTG010000040        20362 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.041, whole genome shotgun sequence.
ACCESSION   DADXTG010000040 DADXTG010000000
VERSION     DADXTG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.041"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IWI57_001248"
     CDS             292..1431
                     /locus_tag="IWI57_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBC2449404.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IWI57_001249"
     CDS             complement(1498..2073)
                     /locus_tag="IWI57_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2449405.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IWI57_001250"
     CDS             complement(2078..3052)
                     /locus_tag="IWI57_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBC2449406.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IWI57_001251"
     CDS             3335..4303
                     /locus_tag="IWI57_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2449407.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IWI57_001252"
     CDS             4416..4694
                     /locus_tag="IWI57_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBC2449408.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IWI57_001253"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IWI57_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBC2449409.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="IWI57_001254"
     CDS             5151..5906
                     /locus_tag="IWI57_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2449410.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IWI57_001255"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IWI57_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBC2449411.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IWI57_001256"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IWI57_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBC2449412.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IWI57_001257"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IWI57_001257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBC2449413.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IWI57_001258"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IWI57_001258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBC2449414.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IWI57_001259"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IWI57_001259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBC2449415.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IWI57_001260"
     CDS             10643..11185
                     /locus_tag="IWI57_001260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBC2449416.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IWI57_001261"
     CDS             11208..12764
                     /locus_tag="IWI57_001261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBC2449417.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IWI57_001262"
     CDS             12778..13680
                     /locus_tag="IWI57_001262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBC2449418.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IWI57_001263"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IWI57_001263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBC2449419.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IWI57_001264"
     CDS             15123..15545
                     /locus_tag="IWI57_001264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBC2449420.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IWI57_001265"
     CDS             16014..16244
                     /locus_tag="IWI57_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBC2449421.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IWI57_001266"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IWI57_001266"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBC2449422.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IWI57_001267"
     CDS             17655..17831
                     /locus_tag="IWI57_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBC2449423.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IWI57_001268"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IWI57_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBC2449424.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IWI57_001269"
     CDS             18326..18709
                     /locus_tag="IWI57_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449425.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IWI57_001270"
     CDS             18878..19762
                     /locus_tag="IWI57_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2449426.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="IWI57_001271"
     CDS             19876..20112
                     /locus_tag="IWI57_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBC2449427.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IWI57_001272"
     tRNA            20200..20280
                     /locus_tag="IWI57_001272"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IWI57_001273"
     tRNA            20287..20358
                     /locus_tag="IWI57_001273"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6762 a         3562 c         4423 g         5615 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta ta
//
LOCUS       DADXTG010000041        20202 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.042, whole genome shotgun sequence.
ACCESSION   DADXTG010000041 DADXTG010000000
VERSION     DADXTG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.042"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..1433
                     /locus_tag="IWI57_001274"
     CDS             6..1433
                     /locus_tag="IWI57_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449428.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1525..1890)
                     /locus_tag="IWI57_001275"
     CDS             complement(1525..1890)
                     /locus_tag="IWI57_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBC2449429.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1887..2252)
                     /locus_tag="IWI57_001276"
     CDS             complement(1887..2252)
                     /locus_tag="IWI57_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBC2449430.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2444..2692)
                     /locus_tag="IWI57_001277"
     CDS             complement(2444..2692)
                     /locus_tag="IWI57_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449431.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2691..2819
                     /locus_tag="IWI57_001278"
     CDS             2691..2819
                     /locus_tag="IWI57_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBC2449432.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2876..4570
                     /locus_tag="IWI57_001279"
     CDS             2876..4570
                     /locus_tag="IWI57_001279"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBC2449433.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4574..4693
                     /locus_tag="IWI57_001280"
                     /pseudo
     CDS             4574..4693
                     /locus_tag="IWI57_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5221..6354
                     /locus_tag="IWI57_001281"
     CDS             5221..6354
                     /locus_tag="IWI57_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBC2449434.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6385..6705
                     /locus_tag="IWI57_001282"
     CDS             6385..6705
                     /locus_tag="IWI57_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBC2449435.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6734..7198)
                     /locus_tag="IWI57_001283"
     CDS             complement(6734..7198)
                     /locus_tag="IWI57_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449436.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7330..8583)
                     /locus_tag="IWI57_001284"
     CDS             complement(7330..8583)
                     /locus_tag="IWI57_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2449437.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8612..8929)
                     /locus_tag="IWI57_001285"
     CDS             complement(8612..8929)
                     /locus_tag="IWI57_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBC2449438.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8955..9275)
                     /locus_tag="IWI57_001286"
     CDS             complement(8955..9275)
                     /locus_tag="IWI57_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2449439.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9739..11154)
                     /locus_tag="IWI57_001287"
     CDS             complement(9739..11154)
                     /locus_tag="IWI57_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2449440.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11171..12487)
                     /locus_tag="IWI57_001288"
     CDS             complement(11171..12487)
                     /locus_tag="IWI57_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2449441.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12637..13335)
                     /locus_tag="IWI57_001289"
     CDS             complement(12637..13335)
                     /locus_tag="IWI57_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBC2449442.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13624..14580)
                     /locus_tag="IWI57_001290"
     CDS             complement(13624..14580)
                     /locus_tag="IWI57_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBC2449443.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14693..15526)
                     /gene="msrA"
                     /locus_tag="IWI57_001291"
     CDS             complement(14693..15526)
                     /gene="msrA"
                     /locus_tag="IWI57_001291"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBC2449444.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15701..16969)
                     /locus_tag="IWI57_001292"
                     /pseudo
     CDS             complement(15701..16969)
                     /locus_tag="IWI57_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17207..17443
                     /locus_tag="IWI57_001293"
     CDS             17207..17443
                     /locus_tag="IWI57_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449445.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            17450..19402
                     /locus_tag="IWI57_001294"
     CDS             17450..19402
                     /locus_tag="IWI57_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBC2449446.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(19474..19971)
                     /gene="dfrG"
                     /locus_tag="IWI57_001295"
     CDS             complement(19474..19971)
                     /gene="dfrG"
                     /locus_tag="IWI57_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBC2449447.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         6462 a         3921 c         3439 g         6380 t
ORIGIN      
        1 ctactatgat tgaagaatat attgagaaaa atattttaag acagctgttt ttatgtgggc
       61 aattctatgt aaataaagaa gtcaatctag aaaagctctc caatctcctg catgtttgca
      121 aaaccacttt actcaatgat atcaataaca taaaaaaaga atttgaagaa cagatcgctt
      181 atacacatcg ggaaaaggac cgttacactt tatatttttc agaacatata cctcgatgca
      241 agatcatgca gcaattgtct caaaattcgt tgtttttaaa aacttgtctg ctttatttgg
      301 aagaagatga acctgattat cttcaattaa ctgaatgtga attcatctct gtcagcaaag
      361 cttattcatt aaaaaaacaa gtattagctt actttaatga ctgcgggatc gaaatcgacc
      421 gctactcccc tcgattcact gaaatggaaa gacgtctgct gcttcttaat gtgtcgtatc
      481 gtttaggcgg ttttaattct tgggagctac ctgaatcgtt ttttgaacgt gccgatcgtt
      541 tcattgaatc cgtaacggaa aatagtggca gattttatga taaagaaaat aaagaaatct
      601 tatcgattgg ttttgctatc agtttcctac gccagcaagt gtgtgctgtg acaatagatt
      661 caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa tcagcatggg
      721 aaaacactga tttccaaacc tattataaaa aagaagaatt cgcctttatt ttgacactat
      781 tcaatctttg taattatggt ttccattctt atcagttgat tgcggaagac tttcaacagc
      841 tacaccaagt gtttattgac aatactcccg aaataaaaga actagttgct acctttgaat
      901 ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga acgtgcgctg attcatctga
      961 tgcgttcggc ttgggataac tatcaattat tcatgccaga aaaattttat cttttaaatg
     1021 aggaacagac aaatttatat aaagaagtcc agacaatttt ttccagctgg tccagccagt
     1081 taccttatga tctacggttg aatcctaatt gcatgcgggc ttttgtgatc gaattgagcg
     1141 gaattctccg tctgacaaaa gaacatctga ctatctatat cgtcacaaat tcggatgttc
     1201 attatctgat ttatcgggaa gccttggaag ctgtaactac tttcgatttt caagttgcac
     1261 caacaattta ttcaagtata tctgatataa agaagtatgc gcagcagtcc tcaaaccggg
     1321 tattatgtga acgtacatta tatacaccag atgcggtcca atatgaaaat attattccta
     1381 tttcgatcaa tacgatcgat cgagctatca tttcagctgt gcaaaacaaa taaggggctg
     1441 tgacaaaaat catgtcacag cctcttattt gttgaagatg cccatttctg taacaatctc
     1501 ttgatacacg tcaggctcac aacctcactt attttggaca tctaggggtc cgatctcttt
     1561 atttaaaaaa taagaaatga tggttcggat aactaccaac gccgctagtt ggaaaatatc
     1621 ctgcaaggta ggtttgataa tcgattcgat gatcccagct gaaacaagaa tttccaaact
     1681 tagaagtata tagcctccca acatcttttt aatagcattg ttctcgctta ctcggtcgct
     1741 ttgcactttt gtatggacaa ataaatattt caaaaaatcc ttgattgata gacaaacacc
     1801 ccaaaccaat aaacagatag caaagatatc aagtcccagt acaagttttt cgaaatatgg
     1861 aagaagaaac tgcatcaact ctgtcgtcat cctacctctc ctcttctttg tttttctttg
     1921 tcttctattt cctgattcaa gaaataagaa atgactgttc gaatgataat aataagtgca
     1981 agcttcaaaa tatctgtcca agtcggcttg ataactgact cgatgatatc agcaacgact
     2041 agtacttcca agcttaacaa aatataactt gctagcagtt ttcttgtatc tgcattacga
     2101 gtattacgtt ctgcatagtt tttcttgaca tgagaccaat caaaaagttt gaatagaaca
     2161 agaacgatcc cgacaattaa gataccgata gaacaaatat ttaaaatagc aatgatccat
     2221 tctagaaatt caattaaatt tgagcgaaac atatacgcta cctactttct tttaatttgt
     2281 tcctattctt ttattgtaaa agaaagttac cttgttcgtc tatttaaaag ttgttctaat
     2341 gataacggtt cgcttaaatt tttctttttc actctcccat ttttctccct ccatccataa
     2401 atcgtggaag tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc gtcatcagct
     2461 tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa atagaaccat
     2521 acacctcctc aaatcttttc ttcttaccta ccctattttt atttacagga aaatcagaca
     2581 cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg cattctaata
     2641 aaaacgaact atccttggat atagaaagag aaaacaagga ggcgtttttc atgagtaaaa
     2701 aattaatcaa agtcggcatc ggtttaggtt tgcttgccct tggcgctgca tatctaggaa
     2761 aaaagacagg actattcgaa gatgacagtc atttatatga tgaattcgaa tcaatctagc
     2821 atcctattta gttgaatcac tacggacata cttggaagac attggaggaa tcacaatgag
     2881 aaaaagtaaa gcaataatga ttggtgccgg tattgccaat atggcagcgg ctgtttattt
     2941 gattcaagaa ggcaaatgga aaggggatca gatcaccttt tattcattgg atgatcacgg
     3001 ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa tattggaata aaaaccatcc
     3061 attagaaaac caaaaaggct atattgctcg aggcggtcgc atgcttaatt accgtaccta
     3121 tgtagatttg atggatctgt taagtcgaat accttctgtt actgagccag ggatgactgc
     3181 tgaagaagat acgagagatt ttgatgcaaa acaccgaacc tttgacaaag cacgtttgat
     3241 ggaaggtggc aaaggaatca ttgatggcgg aaaactggga ttcaataata aagaccgtat
     3301 tcttctgact aaactgattg ctatgcctga ttcagaagaa gaaaaactag acaatatcac
     3361 tatcgaagaa tatttcaaag atgatccaca cttttttgaa acaaacttct ggtttatgtg
     3421 ggaaacaaca tttgctttca gaacacgtag ttctgctcaa gaattgcgtc gctatatgca
     3481 tcaaatgatt tatgaattca ctcaaatcga acatctagtg ggcgtcaatc gaacacgtta
     3541 caatcaatac gaaagtatta tgcttccttt gatcgagtac ttgaaagaac aaggatgcaa
     3601 aatcattttg aaccgccgtg tcactgactg ggaattcaaa gaaacaccga tgcaagatga
     3661 aatcactgtg actggtttga aaattaccaa tacactgact caagaagaag aatacgttgt
     3721 ggtcgatgaa gatacagcag ttattttcac taatgggtcc attacagatt cagctacttt
     3781 aggagattac gaaacaccag ctgtagaaaa tatggattat ggtgcagctt ctagtctctg
     3841 gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct gacaaattct ttgctgatcg
     3901 agatgcaagc gaatgggtca gctttaccct gacgacgaaa aaccatttgt tgttgaatga
     3961 aatcacccgt atcacgactc aagaaccagg aaatgccttg aattcattta tttcaaccac
     4021 accgatcacg ccgttaggcc aaaaagatgt caatatgtct attgtcgtgc atcatcaacc
     4081 gcattttacg tctcagaaac caaatgaaac agttatttgg ggttatttcc tttatccaag
     4141 acgacgtggc gaatttgtgg ataaacagta tattaaaatg aatggaaaag aaatgctaga
     4201 agaactgatt ggacaacttt caaaagtaga tcctggacca gtgaatattc gtgaaaaaga
     4261 aacagaaatt ttcgacagcg taataaataa tatccctgtc tatatgcctt atgcttccgc
     4321 gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc attcctaaac attcgaccaa
     4381 tctggccttt acaggagaat ttgtcgagca gccttatcag atgatcttta ctgaacaaag
     4441 tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg ggtattccta tgtcccgtct
     4501 tgtcaaaaca ccgcgttacg acaaagatat tcctactcta gctcgtgccg caaaaaaaat
     4561 gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacaaccag
     4621 gctaaaacag cttcttatgt ttttgatttg gttggactta ttaccgcaga tgcaagcttt
     4681 tgaacaccgt ttaccaagag gttatgaaaa agctgccctg catcaagtaa taagagcagc
     4741 caagctaaaa cagcttctta tgtttttgac ttggttagac ttattaccgc agatgcaagc
     4801 ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt tagtttcgag tatgaagaaa
     4861 aattttgtga aaatggttcc tcacgttttt gcataaattt gacttaatac cgagaaactg
     4921 atcccttctg tcataacctc tttttattta tgtctgtttc tttttgaaaa caactatttt
     4981 ttcaacgttt actctcttgc caatccaaca tcatctaacg gatacaacta tttcaatgat
     5041 taccattaca ctaaaaaatc ggaaacacac ctctccgaac acaaatacat aagactatag
     5101 aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa ggtaagatct cctttaaaat
     5161 gaaattatct gtagtactaa taatgcagac aattttatga atctgaaagg agcatctcca
     5221 atgaaagcag tcacttggca aggaaaacag aaaatggaaa tcagaaacgt agatgatcca
     5281 aaaatattag aaccaacaga tgcaatcatc cgtatcacag caacagccat ttgcggctcc
     5341 gatctgcatc tttaccatca tggtgaagca gttttggaaa aagggtatgt tgtaggacac
     5401 gaaccaatgg gaatcgttga agaagtggga tctcaagttc aaacattgaa aaaagggcaa
     5461 cgtgtggtta tcccttttaa tattagttgt ggtcattgtc atttctgcct gaatcacatg
     5521 gaaagccaat gtgatgaatc aaatccagat cagttatatg gcgggctttt cggatttggc
     5581 aagttgaacg gtaatcattg gggtggtcaa gcggaatatc taagagttcc ttatgcagac
     5641 tctacttcat ttgttgtacc agataatgat cttcctgatg aaaaagtttt atttctatca
     5701 gatattttgc ccacagctta ttggagcgtt gaaaacgcag gagtaaaaaa aggcgataca
     5761 gttgtgattc taggttctgg tccagtcggt ctattcgctc aaaaattcgc tgttatggct
     5821 ggagcagagc gcgtaatcgc agtagatcca gtacagcacc gtctagataa agcggctaat
     5881 tacaatgggg ttgaaactta tttcttagaa gacacggcac aagctggaac agaattgtat
     5941 gaactggcta aaggtggcgc agatgtcatt atcgattgtg taggaatgga cggattagag
     6001 cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa gcggcacaat ctctccatta
     6061 cagatggcat cgcaagctgt caaaaaattt ggaacagtcc aagtaactgg tgtttatatg
     6121 actccagctt cttcttaccc gctgcaagaa ttttttatgc gggatatcgc tgtcaagcat
     6181 ggacaggcac caattatcca tctaatgcca aaaatttacg caatgattgc agaaggacta
     6241 ttcgatccta cacaaatcat tactcattcg ctgccactat cagaagcagc acaagcatat
     6301 gagatctttg acaaaaaaga agataaaaat ataaaagttg ttttgaaacc ttgaattcag
     6361 taaaaaatac aggtgatttt ctttatgccc aaaatcaaac gaaataccca tgagatcgaa
     6421 ataggaaaag aagaagatat cgaaatacga ggacgtcgct tccgcttcta tttccaaaat
     6481 cgttacacat tgatctcact ggctgtcgat ttattaaccg ggattttcta tatttttgga
     6541 agccttgctt ccttaactaa aatccccgat cgatatggca cctattttta tttggctggt
     6601 gctattttct tgacgattcg tcctattctc agaatcgtaa ggaacatttt tatttacgac
     6661 gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg catgagaatt tttcaaatct
     6721 catgcctctt ttttcaccct atcgtatcac tttgatacag acgagcggtc gtcccacttt
     6781 cttccttgac cattgtcaaa aatgtccact cgtatcgttc ataaaacgtg gtatgatctg
     6841 taattaagta aagtttttcg tacccaagtt gatgcatttc ttttctgatt tccgctaata
     6901 tccgcttggc tatcccctgc ttacgctttt ccggttcaac aaacagggca caaagatttg
     6961 gtgtcagatc agggcggtta tggaagtcgt tttcaatgac gcccgctcct ccgacgatgg
     7021 tttctttttc atctaagacc acataccatt gaggaatact ttccttatta gcgattccag
     7081 tacgaatact ttcctcatac acaacagcag gaactcccca ttttgatgaa aaccaattcg
     7141 cgctttgttt tactaattct gggtgttctc ttaatttgat ccagttatat gtaaccaatg
     7201 tttaaacctc cgactaaaat aaaacgttta caaaaattat acaagtcttt atgaaaatta
     7261 gcaatgacaa caatagtgat gtaaaaaaaa aaacggaaaa caatcacttg ttctccgttt
     7321 tttgattaat caagcatcta aagcttcttt tcttctttct tggatcgtag ctaccttgac
     7381 gaacggtaga tagatcaaaa tagaaagaac caaattgaca gcggctagta caccacctgc
     7441 aatacttttt gttgccaata caccgccaat aattggtgga gttacccatg gaggcataac
     7501 ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa gcaacactaa ctaagaccat
     7561 tggtgtcaaa ataaatggaa tgaacatgat cgggttcaac acgataggta acccaaacat
     7621 cgttggttca ttaatattga agacacctgg cgcaatcgac aagtttgtta caaccatata
     7681 tggtttgttt ttacgtccta ctaaataaat agctaacagt aaacctaaag ttgcacctgt
     7741 tcctcctaag ttgacaaagc tgtcaaagaa aggtttgttc acgataaacg gtgcgctatg
     7801 tccagctgcg attgcattaa cgtttgcttc aatggctggc gcattgattg tttgcatcaa
     7861 aggatcgacc atattcgctc cgtgtaaacc aaagaaccat aagaatggtg tgataaatgc
     7921 aagcagtaag gcagatggat aagagtttgc aagtcccata aatggttctt ggaccactgt
     7981 atagaaagaa cctacgatat catcaacacc gaatgcagca aaaattgcag caactaaagc
     8041 aaacgcagag atagtgatca ttgctggtag taatgaagca aatgcttttg caactgccgg
     8101 tggcacacca tcaggcattt tgatgatcaa tttttcattt ccgcttaaac gtacaaagat
     8161 ttctgttgaa ataatcccaa caataatagc aataaacaaa ccgtttgagc tcatacctaa
     8221 tgctccgcct aaagcgaaga aagagcctaa agaaatcaca cctgcggaaa caccgtcttt
     8281 tcggtagccg ttagctaaat gataagcaag caagaaagca atcaagacag aaagaatggc
     8341 aaatgtaccg ttccaagcag cagccccgaa tcctttccat gattcaccac caaaaattga
     8401 gttcatcaat tcttggaacc cgggaatcgg taagttattg atcattactg ctaatgctcc
     8461 taagatcatt aacggcatac tcaccataaa tgagtcccga atcgcaacta aatgccgttg
     8521 tgcaccaatt tttgatgcta caggtataaa atgtttctcc ataaatgcaa caaatttatc
     8581 cataaatctt ccctcttttc tttactgtag attacgcgtt caatcgacga tacagatcaa
     8641 tgatctcttt tgctaaatct gtaaaagcta tagatgtcat cagatgatct tgactgtgga
     8701 ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc ttgtgtcagc atctctgttt
     8761 gagagtgatg tgcttggact aaagattcct ctgcatcttt gattttttca tctgctaaag
     8821 tgaaatcacc ttttttcgca gcagcaatcg cttccattgc atcgcttttt gcatttccgc
     8881 caaacatgat cagacccatg atggcttcta gatttttttg atcttccatt tattgtatcc
     8941 tccgataatt tttattattt gtttattagt ttttctgctt ggtcgagtac tttttcacca
     9001 ttcatcatgc catagtcagc catattgatt acatctaaag gaatgccttt aggtgctagc
     9061 ttttcagcaa attgattttg cataaagcgt acttgcggtc ctaaaagtaa aacgtctaca
     9121 tttttagatt ctagttgatt atccgcatct gaagcagata cagcaaagat gtctgcttcc
     9181 attccttttg cttctgctgc cttttgcatt tttgtgacta ggagacttgt actcattcca
     9241 gctgaacata caagcatgat tgttttcttt tccattttaa acacgttcct ttctatactt
     9301 acacttgtat ataagcaagt agcgtgccaa ctttaaaagc gctttaaaaa gttgatatca
     9361 tcacatttaa ggtctatacc aagttgcata cacactaaaa aagaaagcgc acactaaaca
     9421 cacaaattaa tttttattac cttttttctt ttaacgtgca caaaaaaagt tgtgacacaa
     9481 aacttgtcac aacttttttc aaataaacgg tattcaaaag cttgcatctg cggtaataag
     9541 tccaaccaag ttaaaaacat aagatgttgt ttttactttg ctattcttat tacttgatgc
     9601 aggacagctt tttcacaacc tctcaataaa cggtgttcaa tcagctgacc ctgggtatta
     9661 gctcaaaacg ctggattcac aacctctttt atttcaaata accagtaaat acaatgaaca
     9721 tctacaaaaa acttctattt atttatagat ttcgtcaggc aaatccaatt ctctcttatc
     9781 aatcaaagtc tgataccacg aagcagattt tttcaatgtt ctgcttcgtt catcgttcaa
     9841 ttcgattcga actaatccat atcgattctt aaatgcattc attggtgaca cacagtcagt
     9901 gaatgcccat aacatatagc cttcgcaatt tgcgccttct tctgttgctt tgatcaagta
     9961 atgcaaatgc tgtccgataa attcgatacg gtaatcatct tgcacgattc ctttctcatc
    10021 catatactgt tcttcatatt ctcttcccat accgttttct gttatcagcc aagggatatt
    10081 atgataattt tcttttagat acatggctat atcatacatg attttcggat agatttccca
    10141 tcccctggaa ttattcattt tcttcccagg taaatcaaag acttcataat atcgttcagg
    10201 atggaaaggt gtctcttcat tccattgata acgaggggcg cgaacgcgtt taggataata
    10261 ttgattgatt ccaaggaaat ctactgtatg atgctgaatc gtttctaaat cctctgttgc
    10321 aggatcgaaa taaatcgatt gtttttgaca caactccagg aactcttctg tgtagtatcc
    10381 ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa tcatacatat aagcagcttt
    10441 ttggtcttct ggagaattgg atcgagcata gaccatttca ggatttaaaa tacagccaat
    10501 ccttccacca taatttccgc tgcggtaaat ctctactgct ttagccgttg ctaaaacttt
    10561 atgataattc cacatcatcc acttttttgt gttttgttca tgcggccagc gaacagcatc
    10621 caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat gtaaaccagt tttttaccct
    10681 atcaccgtaa cgatcaaagg caatttgagc atacttagca taaaaatcaa ctacttttct
    10741 tgaagaccag ccatcatatt tttccaaaag aaaagctggg acttcataat gttccaaaca
    10801 aatcatcggt tctacgtctg cttcgatcaa agagtcgatc atttcactta tatgctgagc
    10861 atattcttca tctactatac agttctcata atcagtaaaa aaacgagccc aattgataga
    10921 agtccgataa tgtgtcagat ggatggattt catcagatcc acatcctgct ggtattgttc
    10981 cataaaatta gttgctacgg ctgggccata cccattatgc caaacaaaat gttcattttt
    11041 ataccagcta tctataaacg aatcctgtcc ttcttttttt cctttccaac cttccgtttg
    11101 ccaagcagaa gatgcagcac caagaataaa attttttgga atagtaattt tcatgatttc
    11161 ctcctgtttt ttagatccca tcattttcca cacgatttgc tacttttaca aatggcagat
    11221 agatcaaaat agaaacaata atacaaacaa gttgcgtaat aactgcaccg atatttcctg
    11281 ctgttgccag ataagcattg acgatcggcg gcattggcca agggaccatt acaactgctt
    11341 tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt tccagtgata agaggcgtta
    11401 agataaacgg aatagctaaa attggattca gcatgatcgg cataccaaaa ataactggtt
    11461 cattgatgtt gaagatccca ggagcaaggg ataacttggt gatttgtttc atatcttccc
    11521 gttttccaac taacaatact gcaatcagta aaccgatcgt gactccagac ccgcccatac
    11581 tcatatacat atcccaaaat ggcatagtaa taatgttcgg tacttctttt ccagcttcaa
    11641 aagcttccgt atttacagca attgccgcta aaagcaacgg ttcccgtatc ggcttcacca
    11701 tttgatttcc atggatacca atcacccaaa agacttgcgc aataaacatc aatagcaaaa
    11761 tacccggtaa cccttgtacg acaccttcta atgggcgttg tacgacattg taaatgatat
    11821 cataaaggta cattcctgta agttctttga tgataaatcc cgcagccgcg atacccgata
    11881 cagtcagaat cgtcgggaat aaagcagaaa aactagcaga aacattcgtt ggcacggtat
    11941 caggcattct cagctgtagt ttcttctgtt tcctcaacca gcaataaagt tcaatagaaa
    12001 ggatggagac caacattccc aaaaataacc ctttcgtatc tgtatattgt ttagccaata
    12061 cgttttctac tataacagtc tgattttcat tcactaattc ataaacagtt ggattaacag
    12121 ataaataaga catcactgcg atgataccag ggaatacacc atccacttta ttcagcttgg
    12181 ctaattcgat ccctatcaga aaaacagcat agatggtcaa gaaacttagt gtgacatagc
    12241 tgatggcaga tgcgataggc tttaattctt ctaaaaaacg caagaaactt atctgtgcta
    12301 agccattttc cgagtcaaag accattgctg aaaacaatgt gccaaaagca cccgtaataa
    12361 ttaccggaat caatgcagca aatgcgtttt ttatcaccat gatatacctt aaattattga
    12421 ttctcgttgc cacgttccct aaaacattgg ctaatttatc cataaaactc attccagatc
    12481 ccctcattcc tgcttcttct ccttgacgtc atctcttttt gtctattagt cagcgcttac
    12541 atttcgatga acagaaacta tttactcctc ttttttacca aatataaaac aaaaattcat
    12601 ctgatgtatt tatcataaaa tacatcagat gaactgtcaa ttagtttctt tttctcctat
    12661 tattttctgt aattctctcc gatttattgc catatggaca gtcattgcat caaacgcagc
    12721 aagtgcattc cgcctttcga tggcctgaac gatctctgta tgaatatcga tcgtctcgat
    12781 tttcagatgc tggatatcat aatttttatt aaacaaagca attgattcat taatgattgg
    12841 gataatatgc agcaaggcta cgtttccgct agcttctgca atcatcgtat gaaaatcgat
    12901 gtctaattga acatgttctt gtgtgccttc tcgaaaagaa gtttcaatct tttgcgttaa
    12961 ttttttcatt tggacgattt gattcttcgt tgcatattcc gctgccaaac ctgccatctt
    13021 aggctccaat aaatatctca gttcaaataa gtctcgtatc atccgatcct gatcttcaat
    13081 caaagaaaaa ccgagtgggt cattggcaat ccctcgctta tcactgacaa atgtccccgc
    13141 tccttgccga acttcaagga tattcctgga agctagagcc cgcacagctt ctcgcaccgt
    13201 gcttcgcccc acctctaaat aatcggacaa ttcatattca ttcggcagtt tatctcctac
    13261 ttgcaactgc atttccttta aatatctaag gatcttgtca gtagttactt ctactagggt
    13321 tttattttct tccaaaccac atacctcctt ttcatctgat gtattttttc gtaattatta
    13381 taacacgttt gaatcatctg atgatttttt gtatacaaaa ttgattaatc cgttcataga
    13441 catacaaacg aagaagatat ttaccataaa aaagactagg ataaaattca ctcctagcct
    13501 tcgatcattt ttattttgaa cgacaaaaaa tatgatgttc aataacagac gatcttcatt
    13561 cgattcttga tgtcatcttt tgtttttttt aatatatgga taaaaagttt gattacatcg
    13621 tttctattct agaatttggt cgattctcaa ttcttcttct ggtgagaaat ctaaattatc
    13681 catcattcga acactttctt gtaactgact taaccgactt gctccgacca gtacacttgt
    13741 aacagatttt tgtcgcaaat tccatgcaag ggccatttgc gctaatgatt gtcctcgtga
    13801 tacggcgatt gtttgcagtg ctttgatttt ttccagagtt gatccaactt gttcttctga
    13861 aaggaatggt atttccttac gatgtgctct agaatcttca ggaataccat gtagataacg
    13921 atttgtcagc aacccttgag caagcggact gaacgtgatt gcccctagcc cttcttcttc
    13981 taatactgga gatagatcgt cttctagtag tgcacgagaa aacatattgt agcgcatctg
    14041 atgaataatg aacggtgctt cctttctttt taaaatttca gtcatttttt ttgtatcttc
    14101 tccattatag ttggatatac caatatataa tgcttttcct tgtcttacaa gttgcatcaa
    14161 agcttctgct gtttcttcca aaggtgtatc agggtctggg cgatgatgat agaagatgtc
    14221 aacataatct agccccattc tttggagact ttggtcacaa cttgaaatga tggatttacg
    14281 agagccccat tccccatatg gcccttccca catatgataa cctgctttgc tagaaataat
    14341 cagttcatca cggtaggctt gaaaatcttc tctaaaaata cgcccaaagt ttttctctgc
    14401 agctccagca gggcctccat aattattcgc aaggtcgaaa tgggtaattc ccatgtcgaa
    14461 tgcgcctctt aaaatctctc tttgattatg gataggatca taatcaccaa aattttgcca
    14521 taatccgaga gataaaatcg gtaatttcaa acctgaatga ccggatttac gatattccat
    14581 tttttcataa cgtgcactat ctgcaatata caatttttct cctcctcatc aaatcttttt
    14641 aaaacgcttt cttttttcta tttttagttt actacatttt aaagaaaagg agttaatcaa
    14701 aatgattttt tcgtatattt ttttgccatt tgttttttac tcgttgatag atcagaaatt
    14761 gattacgtgc ttgctggatt tttttataac gtttcggttg ttttttgtaa aattgctgat
    14821 gatagttttc tgctggccaa aacgtaccgg ctggttcgat ttttgtgacg attggttggt
    14881 ggaaagttcc tgaactgtct agtttctgtt ttgattgttc cgctaattct ttttgtcgtt
    14941 cgacttgata aaaaatcact ggacgatatt gcttccctct atcttgaaat tgtccaaatg
    15001 tatctgtggg atcagtaatt tgccaataga ttgtcagcaa ctcgctataa cttatttctc
    15061 ttgtgtcata aatgatctcc acagcttcca catgacctgt gtaaccacca cttacttgat
    15121 cataatttgg tttttcaaca tgtccgcccg tgtatcctga caaaacagat aggatccctt
    15181 ttttcgattc aaacggctcg accatacacc agaaacatcc ccctgcaaat actgcacgtt
    15241 cttgatagtc agcatcttgc atggcatgct tggaaaagtc atatttttgc tccttatcaa
    15301 atccttccgt aatcaataaa taaaagtcca ttacatcagg cgtcagattc attcggatag
    15361 ctaatggacg tagtgttgct tctagttttg ataactgttc tttaacggat atattttctc
    15421 ttatgtcatt ctttgtctga atcaatactt ttcgttccca atctcgtgta ttcggattca
    15481 aaatcagatt ataaagtgtt tgaatgaata tttgttcctc atccatagat tctcctcctt
    15541 tccgaagaag acaagctagt gactttagct gtaatttatc ataaagtgag gaaataagcg
    15601 attacttcat ccccctacag atttttcgta taaaaaagag agaatcctta tcgacagatt
    15661 ctccttttta atcaatttat tttataaatg aaaagttaat ttacctttta tggaaaacta
    15721 ttttaaaaaa taaacataaa tatgcagtta atgtaacaag ataagaaata atagtcgtta
    15781 ctgtataact tgtgcttact ttaatatcag caactccttt gactccgctt atttgtacca
    15841 atccattagc attcacctgc tcctttacag gttgatgatt tatttctaca ttcgttccta
    15901 aataagcata tacaggaagt gtgattactt ggctcttctc attagttact ctatatatga
    15961 tagaactacc agttgtttta taaggaaggt ttacccattg actgccaatc tttgcttcgt
    16021 gttttattat atgctcctta ttctccaatg aagcgatagg agaataatca tataaaaaat
    16081 aatccattcc tttagtggta tacgcctcta ctgtttcttt gtttacaaca atatgtcccg
    16141 gctgagaaat tattttttga ttgaaatttt ttttactaga aacagttaaa gtgattaata
    16201 acaaaacagt cataaataaa taaaatagtt ctcttgtctt attgtatctt ctatttttga
    16261 aaaatatcat cgttaatatc agagaagaga atattatttg caagcttaag atccgccaag
    16321 gaaattgtaa caatttggct ggcgtatttt gcagaagata ccatggcacc aaactagttg
    16381 tagctagcca agtcaaacac gtaccataat aaatcttttt ttctatgttt gttaattttt
    16441 taaataaaat caggggaata cataaagata acagaccaac tagtcccatt gagtaagtag
    16501 ttaagtcgtt gttcatagct tcagtcagac tatcgaacac attagatgca tgagcatata
    16561 aatttgtcac gctaggtttg ttgatttttt ggtagagcat ttgttcaaac atcggatacc
    16621 aaaaagcact cgtcagaacc aatgtcattc ctgccgcact aaataatttt ttaaatatag
    16681 caataaaatc tgcattcttg aaacctttag aaaccaaacg aataaaaata aaaatgataa
    16741 ttaatgctgt actcattaat gttgatagta catggctgta cactagcaaa ctcatactca
    16801 atgctagctt tacagacgga tacttttctt cttttccaga gatgatttga taagcataaa
    16861 ataatacagg gataacaagt gttattgcga tagcttcggc aattgcggaa cgatagtaga
    16921 tgtctactgt ccgataagca gagaaaatat agagacaact aaataaaaat aagtagttca
    16981 gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt
    17041 tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa
    17101 gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt
    17161 gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga
    17221 gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat
    17281 agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt
    17341 attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa
    17401 gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct
    17461 taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga
    17521 agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag
    17581 gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat
    17641 tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt
    17701 caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg
    17761 tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac
    17821 agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa
    17881 tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga
    17941 atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc
    18001 tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga
    18061 aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt
    18121 tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta
    18181 tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca
    18241 accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta
    18301 cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct
    18361 taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc
    18421 tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt
    18481 caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta
    18541 tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg
    18601 gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac
    18661 aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag
    18721 tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt
    18781 gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt
    18841 aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa
    18901 aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca
    18961 attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc
    19021 tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga
    19081 tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc
    19141 gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac
    19201 aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca
    19261 atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga
    19321 taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa
    19381 agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt
    19441 atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata
    19501 tacatgaaaa tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac
    19561 ctcattccat tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg
    19621 tatttttgtg atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc
    19681 tccgaaaata aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg
    19741 aacaatttca caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct
    19801 gtcaggtaag gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc
    19861 ctttgtagta tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc
    19921 tttgccaatc actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac
    19981 agccttttta actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt
    20041 atcctgcccg ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca
    20101 aaatcacaaa ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg
    20161 taatttggta tgtaagttaa aatgaaaaaa gtagtccatt tt
//
LOCUS       DADXTG010000042        19844 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.043, whole genome shotgun sequence.
ACCESSION   DADXTG010000042 DADXTG010000000
VERSION     DADXTG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.043"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            269..1378
                     /gene="pdhA"
                     /locus_tag="IWI57_001296"
     CDS             269..1378
                     /gene="pdhA"
                     /locus_tag="IWI57_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBC2449448.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1381..2358
                     /locus_tag="IWI57_001297"
     CDS             1381..2358
                     /locus_tag="IWI57_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBC2449449.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2383..4026
                     /locus_tag="IWI57_001298"
     CDS             2383..4026
                     /locus_tag="IWI57_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBC2449450.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4034..5440
                     /gene="lpdA"
                     /locus_tag="IWI57_001299"
     CDS             4034..5440
                     /gene="lpdA"
                     /locus_tag="IWI57_001299"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBC2449451.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5602..5877
                     /locus_tag="IWI57_001300"
     CDS             5602..5877
                     /locus_tag="IWI57_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBC2449452.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5883..6650
                     /locus_tag="IWI57_001301"
     CDS             5883..6650
                     /locus_tag="IWI57_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBC2449453.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6818..8647
                     /gene="typA"
                     /locus_tag="IWI57_001302"
     CDS             6818..8647
                     /gene="typA"
                     /locus_tag="IWI57_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBC2449454.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            8908..10680
                     /gene="recQ"
                     /locus_tag="IWI57_001303"
     CDS             8908..10680
                     /gene="recQ"
                     /locus_tag="IWI57_001303"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBC2449455.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10794..11096
                     /locus_tag="IWI57_001304"
     CDS             10794..11096
                     /locus_tag="IWI57_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBC2449456.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11099..12262
                     /locus_tag="IWI57_001305"
     CDS             11099..12262
                     /locus_tag="IWI57_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBC2449457.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12288..15716
                     /locus_tag="IWI57_001306"
     CDS             12288..15716
                     /locus_tag="IWI57_001306"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBC2449458.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15737..16861
                     /locus_tag="IWI57_001307"
     CDS             15737..16861
                     /locus_tag="IWI57_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449459.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16851..17288
                     /locus_tag="IWI57_001308"
     CDS             16851..17288
                     /locus_tag="IWI57_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBC2449460.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17272..17610
                     /locus_tag="IWI57_001309"
     CDS             17272..17610
                     /locus_tag="IWI57_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBC2449461.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17729..18289
                     /gene="rsmD"
                     /locus_tag="IWI57_001310"
     CDS             17729..18289
                     /gene="rsmD"
                     /locus_tag="IWI57_001310"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBC2449462.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18286..18777
                     /gene="coaD"
                     /locus_tag="IWI57_001311"
     CDS             18286..18777
                     /gene="coaD"
                     /locus_tag="IWI57_001311"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBC2449463.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18767..19810
                     /locus_tag="IWI57_001312"
     CDS             18767..19810
                     /locus_tag="IWI57_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBC2449464.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         6708 a         3469 c         4379 g         5288 t
ORIGIN      
        1 agatggttgt aagtttgatt agaaaataga aaaaatgcag atgaaatata aaacgctttc
       61 cttattgtga ttttattcac taatgctgtt aatatgcgga ttggcataat ttcctttcat
      121 cgtgaaaatt tgtgctttta gcttgtattt ctcataaatc catgataatt tattaatgaa
      181 gacggttaaa tagtgataca gttttattca ctgtttcaat cgcagaatga agagattttc
      241 ttatagatga gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca
      301 agcactgcta gatgaagtca atgcagattt cccagtatat caagcacttg atcaagatgg
      361 aaacgtagtt aacaaagatt tagtacctga tttatccgat gatgaactcg ttgaattaat
      421 gactcatatg gtatggtctc gtgtattaga ccaacgttca acagcgctga accgccaagg
      481 acgcctaggc ttttttgcac caactgctgg acaagaggca agtcagctgg ctagtgctta
      541 cgcatttgat aaagaagatg ttcttcttcc aggttatcgg gatgtacctc aactagtaaa
      601 acatggttta cctttatctc aagctttttt atggtctcga ggacatgctg caggtaattc
      661 ttatccggaa gaattgaaag ctctaccacc acaaattatc atcggtgcac aatatgtaca
      721 agcagccggt gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta
      781 tactggtgat ggcggttcat cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc
      841 ttaccatgca aatgccgtat tttatatcca aaacaacggg tatgcgatct ctacacctcg
      901 tgaaaaacaa actgcagcaa aaacacttgc acaaaaagct gtcgcagctg ggattcctgg
      961 tattcaagtc gatggaatgg atccgcttgc agtatatact gtatctaaaa tggcacggga
     1021 atgggcgatc agtggaaatg gaccagtcct aatcgaaaca ctgacttatc gttatggacc
     1081 acatacacta tctggtgacg atccaacccg ctaccgtaca aaagacacag atgacgattg
     1141 gcagaaaaaa gatccattga tccgcttccg taaatatttg acggaaaaag gactttggtc
     1201 tgaagaaaaa gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag cagctattgc
     1261 tgaagccgac aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt
     1321 atctccacaa tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt cgaaataatc
     1381 atggcacaaa aaacaatgat ccaagcaatc acagatgcct tagcacttga actagaaaat
     1441 gacgagaatg tcgttgtttt tggtgaagat gttgggaaaa acggaggagt tttccgggca
     1501 actgaaggat tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc tttagctgaa
     1561 tctggaatcg caggattgag tttcggtctt gcgttagaag gatttcgtcc agttcctgaa
     1621 atccaattct ttggttttat tttcgaagca atggacgaag tagtagctca aatggctcgt
     1681 acaagatatc gaatgagcgg cactagaaat ctacccatca caattcgttc gccatttggt
     1741 ggcggtgttc atacaccaga attgcattcg gataatttag aaggtttgat cgctcagtct
     1801 cctggtattc gagtagtgat tccttccaac ccttatgatg caaaaggact attgatttct
     1861 tctattagaa gtaacgatcc ggttgttttc ctagaacaca tgaaactata tcgctcattc
     1921 cgagaagaag tgccggatga agcttacgaa gttcctttgg ataaagcagc agttactcgt
     1981 gagggaacag atgtatctat tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca
     2041 gcagacaatc ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg tacagttgcg
     2101 ccattagatg tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc
     2161 caagaagcac aacgacaagc gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc
     2221 gctatccttt cattagaagc cccaatcgga cgagtttctg cacctgatac agtcttccca
     2281 tttggacaag cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa
     2341 gaaatcgcgg aattttaaga aaagaaggga cgaaacaaag tcatggctta tcaatttaaa
     2401 ttgccggata tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca
     2461 ggagatacga tcaatgaaga tgatacatta ctagaagttc aaaatgacaa atcagtagaa
     2521 gaaatcccgt cacctgttac aggaacggtt aaaaatgtga ttgttccaga aggtaccgta
     2581 gcaaatgttg gggatgtatt agtagaaatc gatgcaccag gacacgaaga caatgaaggg
     2641 gacagtggtg tagccgcaga atcccaaact ccagctaaac cggctgctga acccactgtt
     2701 gatacagaat cagctggatc gtcaagcgaa ggagtcttcc aatttaagtt accggatatc
     2761 ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc
     2821 aatgaagatg atacattact agaagttcaa aatgacaaat cagtagaaga aattccgtca
     2881 cctgtcacag ggacagttaa aaacgtgatt gttccagaag gcactgtagc aaatgttggg
     2941 gatgtattag tagaaatcga tgctcctgga cacaatagtg catcgtcaac tagtgcgcca
     3001 tcagctgaag caccaaaaga aaaagtggaa acatcaggtt cagcaagtgt cgttgaagct
     3061 gccgatccaa ataaacgtgt attggctatg ccatctgttc gtcaatttgc ccgtgaaaaa
     3121 gatgtagata tctcacaagt aactgcaact ggaaaaggtg gcagagtaac aaaagaggac
     3181 atcgaaaact tcttggcagg tggtccaagc agtgcaccag cgaaatcaga agcgccagaa
     3241 gcggcagcac cgaaagaagc agctcctgca gcagaaagca aaccagctgc accagcaaaa
     3301 ccattcaagt ccaacttagg agacttggaa gaacgtgtag caatgacgcc aacacgtaaa
     3361 gctatcgcta aagcaatggt caacagtaag catacagctc cacacgtgac attgcatgat
     3421 gaagtagaag tgtctaaact atgggataat cggaaacgat ttaaagaagt tgctgctgca
     3481 aatggtacga aacttacttt cttgccatat gtcgtgaaag ctttaacagc gactgttaag
     3541 aaatacccag tattgaatgc atctatcgac gatgcaaacc aagaaatcgt ttataaacat
     3601 tactacaata tcggtattgc aactgatacg gatcacggac tatatgtacc aaatgtgaaa
     3661 gatgcagacc gcaaaggaat gtttgctatt gcagacgaaa tcaatgagaa agcaaaatta
     3721 gctcatgacg gcaaactatc tgcagaagat atgcgcaatg gaacaattac gatcagcaat
     3781 atcggatctg taggtggcgg ttggtttaca ccagtcatca actatccaga agtagctatt
     3841 ttaggtgtag gaacgattgc acaacaacca atcgtaaatg ctgaaggcga aatcgttgtc
     3901 ggtcgtgtga tgaaactgtc attaagtttc gatcaccgta tcgtagatgg agcaactgcg
     3961 caacaagcaa tgaataatat caaacgtctt ttagctgatc cagagctatt aatgatggaa
     4021 ggatgataaa caaatggtag ttggagattt tgctgtagaa ttagatacag ttgtaattgg
     4081 tgcgggacct ggtggctatg tagctgccat ccgcgctgct gaaatgggac aaaaagtagc
     4141 aatcattgaa agagagtata ttggcggtgt ttgtttgaat gtcggctgta ttccttcaaa
     4201 agcactgata gctgcaggac accattatca agagtcacta gattctgaaa tgtttggagt
     4261 aactagtgaa aacgtcaaac tagattttgc aaaaacacaa gaatggaaag aaaacaaagt
     4321 tgtccatacg ttgacttctg gtgttggttt cctattaaaa aaacataagg ttgaaacaat
     4381 cgaaggagaa gcgttcttcg tagatgatca cacactacgc gtcattcatc cagattcagc
     4441 tcaaacttat tcgttcaata acgcaatcat cgcaacaggt tcccgtccaa tcgagattcc
     4501 aggatttaaa ttcggaggac gtgtgttaga ttctactggc ggattagcat tgaaagaagt
     4561 acctaagaaa tttgtgatca tcggtggcgg tgtgattggt gccgaattgg gcggagcgta
     4621 cgcaaactta ggagcagaag taacgattct tgaaggctca ccacaaattt taccaactta
     4681 cgaaaaagat ctagtaaaat tagtagaaga tgatttcaag aaaaaaggcg taacagtcgt
     4741 aacgaatgca atggcgaaag aagcagtcga taacggtgac agcgtgacag tgaaatatgc
     4801 agtggacggg aaagaagaat ccgttactgc tgattatgtg atggtcactg taggtcgtcg
     4861 tccgaataca gatgatatgg gcttagaaca agcgggtgtc gaagtaggag aacgaggatt
     4921 gatcacggtg gataaacaag gacgtacgaa cgtacctaac atctatgcca tcggtgatat
     4981 cgttccaggg gcggcattag cacacaaagc cagttatgaa gcaaaaatcg ctgctgaagc
     5041 aatctctggc aaaaaagtag ctgtagatta taaagcaatg ccggcagtag ccttcactga
     5101 tcctgaattg gcttctgtag gtatgacgat caaagaagca aaagatgctg gtatcgaagc
     5161 aaaagcatat aaattcccat tttcagggaa cggtcgggca ttatcgcttg gtaaaacaga
     5221 aggattcatc cgtctagtta cgacgatcga agataacgtg cttattggcg cgcaaatcgg
     5281 tggagtcggc gcaagtgata tggtctctga attagcttta gcaatcgaat caggaatgaa
     5341 tgctgaagat atcgccttga cgattcatcc tcatccatct cttggcgaaa tcgtcatgga
     5401 tgcttcggaa ttagcattag gtttaccaat ccatatttga tttgaataat acaggcgaaa
     5461 cgcaactgaa aaatcagttg cgttttgttt tttagaatta tcaataatat tattatgtaa
     5521 acttaaaaat atgacaactt cttggtttat taccacttcc tatgctacac tgaagaggaa
     5581 tatgaaatag gagagttgaa gatgcaagaa tatcagtatc cacttgattt agattggaca
     5641 acagacgaaa tggtcattgt aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa
     5701 gggataaaca atgaagcttt tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc
     5761 attggtgagg aaaaacgatt gggacgggaa tttgaaaaag cttccggtta ttctttatat
     5821 cgtacagtgc agcaagcaaa aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat
     5881 aaatggaaca gatgattgat catatcaagt cctggcttga agaagctggt gagaaaatca
     5941 aatcttctca acaaacatta acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa
     6001 atgtcgatcg aaacatccag aattttctaa tcgaaaaaat ccattccttt gatccagagg
     6061 cgaaaatatt ggcagaagaa gaagggtgta atcaattatc ggatctcgat ggaagagtct
     6121 ttatcatcga tcctattgat ggcaccttga attttgtgat tgaaggggaa aatttttgta
     6181 ttatgctcgc tgtttatgaa tccggcgttg gaaagcttgg tttcatttat gacgtgatga
     6241 gaaatgaact tttttggggt ggaaaaggaa taggcgtgta taaaaatgat gagccattgc
     6301 ctagaccagc aaataaatcc ttatcggaag gcttacttgg tgtgaatagt tatcttttcg
     6361 gtcataataa atatggaatc cgagaaattg cggaaagcag catggggaca cgtatgtacg
     6421 gatgcgctgg tcttgaacta gtggcaatct tgaaaggaaa ccacattgct tatatttcaa
     6481 accttagccc ttgggattat gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa
     6541 caagcggtct ttttggtact cctttgacat ttgaaaaaag ggaatactat ttagcaggga
     6601 cttcttgtgc atatgcagaa atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta
     6661 gaaatcattc acaaaaatga taaaaacgag caaaagatta attttctcga aaaaatgaaa
     6721 acattttact atatttcatg aaaaaagtct gctataatta gcagtcgagt aaaatttgcc
     6781 ctgtcataaa gaaccaataa aaaaggagtt agttacattg aattatagaa acgatatccg
     6841 taacgtcgca attattgccc acgttgacca cggaaaaaca acattagttg atgaactatt
     6901 aaaacaatca gatacattag acgcacatac gcaattgcaa gaacgtgcga tggactcaaa
     6961 tgcgctagaa aaagaacgtg gtatcacgat tttagccaaa aacacagctg tagactataa
     7021 aggtattcgc gtaaacatca tggatactcc aggacacgcg gacttcggtg gcgaagtaga
     7081 acggatcatg aaaatggttg acggcgttgt gttggtcgta gatgcgtatg aaggaacaat
     7141 gcctcaaaca cgttttgtat tgaaaaaagc actggaacaa catatcacac caatcgttgt
     7201 cgtaaacaag attgacaaac cttctgctcg tcctgagcat gtcgtagatg aagttcttga
     7261 actattcatc gaattaggag cagatgatga tcagttagac tttcctgtga tttatgcatc
     7321 tgcactaaat ggtacgtcaa gcttgtcaga tgatccagca gatcaagaac caaccatggc
     7381 acctattttt gatacaatta ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc
     7441 attacaattc caagtttcat tacttgacta caacgattat gtaggtcgta tcggaatcgg
     7501 acgtgtattt agaggaacaa tcaaagtagg agaccaagta gcattaatta aattagacgg
     7561 aacagttaag aaattccgtg taacgaaatt atttggtttc tttggactaa aacgtctaga
     7621 gattcaagaa gcaaaagctg gagacttgat tgctgtctca ggtatggaag atatctttgt
     7681 cggtgaaaca gttactcctg tggatcatca agatgcgtta cctatccttc acattgacga
     7741 accaacattg caaatgactt tcttagttaa taattcacca tttgccggtc gagaaggtaa
     7801 attcgtaacc gctcgtaaga ttgaagaacg tttgatggca gaacttcaaa cagatgtatc
     7861 cttacgagtt gaaccaacaa actctccaga tgcatggaca gtttctggtc gtggagaatt
     7921 gcatttgtct atcttgattg aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg
     7981 tccagaagtt attgaaaaag aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca
     8041 aatcgataca ccagaagaat atatgggtag tgttatcgag tccttgagct tacgtaaagg
     8101 tgaaatgcaa gatatggtcc atacaggaaa cggacaaatc cgtttgacat tcttaacacc
     8161 tgcccgcggt ttgattggtt attcaactga attcctttca atgacacgtg gttacggaat
     8221 catgaaccat acttttgacc aatatctgcc aatgcttcct ggtcaaattg gcggacgtca
     8281 ccaaggggcg cttgtttcga tcgatacagg aaaagcaacg acttattcta tcatgagtat
     8341 cgaagaacgc ggtacagtct tcgtagaacc aggtacagaa gtatatgaag gaatgattat
     8401 cggggaaaac agccgtgata atgatttgac tgtcaacatc acgaaagcaa aacaaatgac
     8461 aaacgtacgg tctgctacta aagaccaaac atctgtcatc aaaaaaccaa aacaattgac
     8521 gttggaagaa tctttggaat tcttgaatga tgatgaatat tgcgaagtaa caccagaaag
     8581 cattcgcttg agaaaacaaa ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa
     8641 aaaataatct gtaaaaggat gaagagagaa accagcttgt tataaaaaat agctggtttc
     8701 tttttttgtc caactctttt ctgtggagaa gtcatctttg tcaatggaca taaattcaga
     8761 aaatacgccg aaagacgttt aaaagacttt taaaaaactt atttctagag agtttttgtt
     8821 tcataataag ataatgtttc acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt
     8881 aaaatggcaa gagaaaggag cgggaagatg aacgacttat taaaacaata ctttggttat
     8941 gatgagttta gacctggtca aaaagaaatt atccagaaag tcattgatca ggaaaatgta
     9001 ttaggaatca tgcctaccgg aagtgggaaa tcaatctgtt accagcttcc agctttacta
     9061 ttagatgggc ttactgtggt tgtgtcccct ttaatctctt tgatgaagga tcaggtggac
     9121 gcggcgaatc aattaggtat acccgccact tttatcaata gctcgttaga tggttacgaa
     9181 acagctagac gcttccaaga aatcgataga cagcagtacc gtttacttta tatcgctcct
     9241 gaacgtttta tcatgccgga ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc
     9301 gctattgacg aggcacattg tatctctcag tgggggcatg atttccgccc aagttattta
     9361 cagatggcga accagctaga tcaactgcca aatcgtcctg taattgttgc tttgacagct
     9421 acagcgactg tgcaagtagc tgctgacata aaaagacttt taaaaattcc agaaaacaac
     9481 catattcaga ctggttttga acgagaaaat ctacgatttc aagtaattaa agatcaaaaa
     9541 aaagaacaat atctaatcga atatttaaag atcaacaaaa atcaatctgg tatcatttat
     9601 gcagcaacga gaaaagaagt agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc
     9661 gtgggtagat atcatggtgg tctaaatgag aatgagcgaa cagaaatgca agaagccttt
     9721 ttatacgatc gcttgcagct gattgtagcg acaaatgcat ttggtatggg tataaataaa
     9781 agcaatgtcc gttttgttat ccattatcaa attcctggct ctctagaagc atactatcag
     9841 gaagctggtc gggcgggaag agatggtctt tccagtgaag ccatcctgct tttctctcct
     9901 caagatatcc aagtccaaaa gttttttatc cagcagtccc aaagagaaga agggcaaaag
     9961 caaaaagaat atgaaaaatt aaaagcgatg acagaatacg tgtatattga gtcctgtctt
    10021 cagcaataca tcctaaacta ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac
    10081 tgtttagatg acagagaaat agtagaagta acgacagaag cacagatggt tctttcttgc
    10141 ttaaaacgta tgggtgagaa ctatggtaaa caaatgctga tgaaagtctt agcaggatcg
    10201 aaagaacaaa agctgcaagc tttaggtttt ggacatttat ctacctatgg actgatgaaa
    10261 aaccaatcgc aaaaagaaac gatgcagtta attgaatatt tgatatcgaa tggctatctt
    10321 ctgaccatca atggtgagta tcctgttttg aaagtaacag aaagagggat acaagtattg
    10381 aaaggacaag aatctgttta tcgaaaggaa cccaagaaag tccagcagct atcggatgaa
    10441 gaaaccgata cactatttga agtgctgcgg gaactgcgaa cggatctagc atcagaagca
    10501 ggcgttcccc cgtatgtcgt attttcagat tccacattga aagaaatgag ccgaatacgt
    10561 ccatcaagca gattggagat gcttcagata aaaggagttg gccaaagcaa gcttgataaa
    10621 tatggcgaag cctttttatc tcggataaaa aatgcggact ctaacgttct ggataagtag
    10681 aaagaacgaa tacgtaaaaa tgaataaaaa caactggtta aatctgtttt ttttgctcct
    10741 tatgaaaatc aaatatgtta taattaagga attagaatgg gaggtaagca cagatggatt
    10801 ctttgtcgaa aaaacttgcg atccaagtat taaatgatga agcagaacgg atcaaacgat
    10861 taatccgaaa tcaaaacaac agtctctgta tctcacaatg caaagccttc gaagaagtgg
    10921 tagatacaca gatgtacggc ttttcccggc aagtcggttt tgctgtccgt ataggagtaa
    10981 tcggaaagaa cgagggccag ctgatgttaa gtgagctaga acgagaattg aatcagttat
    11041 atagtgaggt ttaccaagaa atttatgata aaaaagaagt tggcaaggag gaatagactt
    11101 gcctaagaaa gtgaaaaaaa ggcatctatt agactatagc atcttgattc cctatttaat
    11161 tttatgtgta ttgggattga tcatggtata tagttctacg tcgtatctat tattagaaaa
    11221 cggacaaaat ccgtccgcat cagtcattaa ccagagtatt ttttgggtgc ttagcttgat
    11281 tgctatcgca ctactttata aaatgaaaac agatgttttg aaaaaccagc gtttgatcat
    11341 ggcagccatc gcagttttga cgattttgct gctgattgtt gtgttttttg gtaaggagat
    11401 caatggggcg aaaggttggc tgcaaattgc aggattctcg atccaacctg ccgaatacct
    11461 aaaaatcatt tctatctggt acctgtctct gactttatct aaaagacaaa acagtgtaca
    11521 aaaagatttt cttgggactg tgaaacgtcc acttgcaatg gttatcggac ttactgcatt
    11581 agtcgctatt cttcccgatt ttggtaatgc ggcagttatc tttttgatta ttttggttct
    11641 gcttttagcc agcggagtca attatgtgta tactttgatt gtaggagtag gaggcttttg
    11701 tctaagtact tttacgatct ggctgatcaa tatcacaaac ggaaagatct tacccggacg
    11761 tttacagtat atctataatc gatttgctat ttaccaaaat cctttctcag acgaattaaa
    11821 taaagggcat caactagtca acggctacta tgccatgttc aacggaggac tatttggcag
    11881 gggattaggt aacagtatcc agaaaaaagg atttttacaa gaagcacaaa ccgattttat
    11941 ttatgcaatc gtagtggaag aactgggagt gattatgggg attttgatat tagcgttgct
    12001 attctttatg attgtgcgca ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc
    12061 gcttttgtgc attgggatcg gcgcaatgtt tttgatccaa gtatttgtca atcttggagg
    12121 aattactggc gttattcctt tgacagggat cacgttccca tttttaagtc aaggaggctc
    12181 cagtttgctg atgctttcga tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa
    12241 acgcaagagc ttaggtttat aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt
    12301 tagttgctaa caggggtgaa atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa
    12361 ttaaaactgt tggaatctat gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg
    12421 atgaagcata tttagtagga caaggaaaaa aaccgattga tgcgtatcta gatattgaag
    12481 ggatcatttc tatcgcgaaa gaatgcggag ctgacgctat tcatccgggt tacggtcttt
    12541 tgtcggaaaa cttgaatttt gcgcaacggt gcgaagaaga aggcatcatt tttgttggac
    12601 ccaaactgca tcatttagat atttttggag ataaaatcaa agcaaaagct gcagctatcg
    12661 aagcggggat tgcttcgatt ccaggcacag atggaccaat cgcttctata gacgatgcct
    12721 tggagtttgc gaaacaatac ggttatccga ttatgatcaa ggcagctcta ggcggcggcg
    12781 gacgcggcat gcgtgtagct cacgatgaaa agagtgcaag agagggctac gaacgtgcga
    12841 aaagcgaagc aaaagcagcc tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta
    12901 atcctaagca tatcgaagta cagattctcg gggatacgca tggaaacgtc atccatcttt
    12961 ttgaaagaga ttgttctgtt cagcgccgtc accaaaaagt agtagaagta gcgccttgtg
    13021 tctctatgaa tgaacagcaa cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat
    13081 atgtcgggta tgtcaacgca ggtacagtag aatttcttgt tgaaggagat gatttttact
    13141 ttatcgaagt caaccctcgc gtccaagtgg aacatacgat cacagaaatg attacagata
    13201 ttgatatcgt aacaacgcaa ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg
    13261 gattgcctca gcaagaagga attaaattaa atggttcagc cattcaatgc cgaatcacta
    13321 cagaagaccc gttgaataac tttttaccag acacagggaa aatcgatacg tatcgttcac
    13381 ctggtggttt tggcgtacgt ctggatgtcg gaaatgctta cgcgggctat gtagtgacac
    13441 cgtattttga ttcattgcta gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca
    13501 ttcagaaaat ggaacgttgc ttgaaagaat ttagaatccg tggagtgaaa acaaacattc
    13561 catttatgct caatgtcatc acacatcctg aattccaatc aggaaatgca aaaacaacct
    13621 tcattgatag tacagcaaca ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa
    13681 cgatgaaata tatcggtgaa attactgtga atggttttcc aggtatcgaa agtggcgaaa
    13741 aaccatttta tgaagaacca cgtatgccta aagatctaat cacaagaact gattacgtaa
    13801 cggctaagaa tgtattagat gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac
    13861 aggaaaattt gttgttgacg gatacgacat ttagagacgc ccatcaaagt ctgctagcaa
    13921 ctcgtgttcg gacaaaggat ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg
    13981 aactcttttc aagtgagatg tgggggggag caacatttga tgtggcttac cgctttttga
    14041 atgaagatcc ttggcaacgg ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc
    14101 aaatgctttt cagaggttca aatgccgttg ggtattctaa ttatcctgac aatgtattgg
    14161 tggaatttgt caaagaagca gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt
    14221 tgaactggac tccacaaatg gaaaaaagta tccaagctgt acgggatact ggtaaaatcg
    14281 cagaggcagc tatttgctat actggcgata tcaacgatcc aagtcgggca aaatacaatg
    14341 ttcagtatta taaagacatg gcaaaggaac tagaacaact gggtgcgcat atcattgcaa
    14401 tcaaagatat ggctgggctg cttaaaccgc aggcagctta tcgtttgatt agtgaattga
    14461 aagaaacgac agatctgcca atccatctgc atactcatga taccagcggg aatggaatca
    14521 tcacttattc tgctgcttca aaagcaggag tagacatcgt tgacgtggca atgagtgcaa
    14581 tgagtgggaa tactagtcag cctagcatga gcagcttgta ttatgcactg gtcaatggtc
    14641 cacggctgcc agaaatcacg attgaaaatg cacagaaatt aaaccattat tgggaagatg
    14701 tgcgcatgta ttacaaaccg tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata
    14761 tgcatgaaat gccaggagga cagtattcga atctacagca acaggcaaaa gctgtcggat
    14821 taggtcatcg ctgggatgaa atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg
    14881 atatcgttaa agtaactcca tcatctaaag tagttggaga tatggcacta tttatggtcc
    14941 aaaatgatct tacagaagaa gatatttatg aaaaaggcga aacactaagt ttccctgaat
    15001 ctgttgtgac tttcttccaa ggagaactag ggcaacccgt tggcggattt cctaaaaaat
    15061 tgcaaaagat tattcttaaa ggcagacctg cattgaatga acgtccaggt cttttggctg
    15121 aatcagtcga cttcaacgaa gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa
    15181 aacaagacga agtattaagc tatttgatgt atccgcaagt ctttttggac tatcaaaaag
    15241 catataatca atttggcgat gttacattac tggacacacc aacatttttc caaggtatac
    15301 gtttgggtga aacaatcaat gtacaaatcg aacgcggaaa aacgcttatc attcgtttag
    15361 atgaaatcgg agaacccgat atcgaaggga atcgcgtact gttcttcaat ttgaatggac
    15421 aaagacgtga aatcactatc aaagataatt cgatcatcag tacggtccaa acccgattga
    15481 aagctgaacc gacaaaccga gaacaaattg gagcaacgat gtctggttct gttttggatg
    15541 tattagtgaa aaaaggcgat aacgttaaaa aaggtgatac gttgatgatt actgaagcga
    15601 tgaaaatgga aacggccatc gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt
    15661 cttcaggaga cactatttcg tctggtgacc ttttgattga agtgactgag aaataataag
    15721 cgaaaggtgg ggaaacatga aaagactggg tttgttttgg ctgattctgc tattagttat
    15781 tgtggcattc tacatggaac cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc
    15841 tgatcaacaa atatcaaacc aaatgacaac gtggaaatac caggagctta aagcaactgg
    15901 tttttctact tatatcggaa aaccgatcga tcagctggaa aaggattttg gccaagccta
    15961 tgatcgatta actagcggat ttggttttga aacaagatat tatgaaaata aagctagtca
    16021 tctcacattt gaagcgaaca tccaagacag cagagtcaca actgttaaag tactgcaaac
    16081 agggcctgct gatatcgcgc cgttttattt agggatgacg atgcaagatc ttacgaaaat
    16141 cactactatc tatactaatt tcacttttga gtaccatgat acagaagttg gaatcgaact
    16201 aatggaagag gatatgaatt atcgtccgct aattgctttt gataacgaca cttttgctat
    16261 tttatttttt gatcaaacaa caaacggcct attttcagtt gtgtatttag ataaagatag
    16321 tcttttgaaa ctattgccat atcaagtgtt tggtgaaggc cttccacatt atcaagcaga
    16381 taaatcggct gattgggaat caatcaatcg cgttaaagaa cgaaaaagta cgactttatt
    16441 aaatatgctt agaagagaag acgaacttcc catatacaat caaacacttg cctttcaaaa
    16501 ccacagtcag ctgctactac atgatttttt gacaaggcct gaggaaatat tgtcagagga
    16561 acgtatggct gattggcaaa aatcgctagg cagtgctcaa acgacagtga aattctcact
    16621 gacgaatgat gaattggaga agttaatcag taaacaaaaa ttagaaaata ctagtgggct
    16681 atttattcat ccggcgattg acgctacttt ttcgttttta tattggtaca gcgatcctta
    16741 caaccatgac cgtttcatgg acccgggaac ggattctgta ggcattgctt tttctaaaga
    16801 aaatatgtta gtcttattgc aagaagaaaa cggagaaagt agtgatagca gtgatcgtta
    16861 acgaagagct gtttcaactg gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga
    16921 gtaaaacgat ggcttcttat cttgaacata aacagtcaat ggaagaatct gcaaaagttt
    16981 gccagttgaa agaagcgttc ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg
    17041 gtgagtatgc acctgattat cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc
    17101 ttgatatgga tgagcatgtt gcagctttcc gatatagtga aacacagctg caaagtctgt
    17161 tggatcaaat tgtccaagta cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag
    17221 gtagcccgtt ttttgaaacg aaaaaacact ttggatgcgg aggtaattgt catgcaatta
    17281 gacaatagtg aatttgaaat aacaaaacga cgcgggctga tcgtatgggt ctacagctta
    17341 aaacaattaa agaatctgaa aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa
    17401 tatgtcgtgc tttacttaaa tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa
    17461 ttacattttg ttcgaaacgt agaacgttct taccgaccgg atatcgaaat gaattttgcc
    17521 gaaaaaatag gaaacaaaac agtattaaaa gacgaaggtt tcgaaattga agagctaaat
    17581 acagaaatcc gattagctga acatctttaa aaatctctta tgagcagttt ttaaataaaa
    17641 aataacttgg ataacgatcg atttttgatc attgtccaag tatttttttg gtatactagt
    17701 accagtacca tttctaagga gtgagtagat gcggataata tcaggaaatt atggcgggcg
    17761 ccgtttaaaa agtttagcag gggcgaacac cagaccaact acagataaag taaaagaatc
    17821 catattcaat atgattggac catattttga tggagagaca gtattggatc tgtttgcagg
    17881 aagcggtggt ttagctatcg aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga
    17941 caaaaattac caagcgctaa aaatcatcaa agaaaacatc gaaattacga aagaaccaga
    18001 gaaatttacc acaataaaac ttgatgcgga caaagcaatc gaacggctag cagcggagaa
    18061 acaacaattc gactacttgt ttcttgatcc gccatatgca aaacaaaaaa taatcgatca
    18121 aatccataaa atggaagaac tgggactttt ttctaaaacc gcagttatcg tatgcgaaac
    18181 ggataaaaca gtcgaattgc cagaaactgt ggctggattc catcaaataa gaaaacagat
    18241 ttatggcata acggccgtaa ctatctatag aaaagaggga aatcaatgaa acgtgcatta
    18301 ttcccaggaa gttttgatcc ttttacaaaa ggtcacttag atacggtgga acgtgcggct
    18361 aaattattcg atgaggtagt aattggtgtt tttatcaata cttcaaagaa aagtttgttt
    18421 cccccagaag aaagaatgac attgatcaca aaagctgttt ctcatcttcc taatgtaaaa
    18481 gtcatgcatc aagaaaatca attgacggta gaaacagcaa aagagattgg tgcagatgcg
    18541 ttgatcagag gcatacgaag tatcaaagac tttgaatatg aacgagagat tgctcagatg
    18601 aatcatcatc tatatgatga attggaaact gtcttcttat tagcaaaacc agaatatagt
    18661 cacgtgagtt ccagtatttt aaaagaagta ctgcactttg gcggagatgt gagcagctac
    18721 ttaccgccag tgatcaacga agctttagct ggaaagcgtg aatcaaatga aagctaaaca
    18781 atggttgaaa acagtactga ttatcatatt tggaatgttg atcattggat ttgtagtagt
    18841 tcccttgccg tattatgtag aatcaccggg agccacgatc gatctgaaag aactaattac
    18901 ggtcaatgat aaggaagata aaggttctgg gtctttctct cttacctctg ttggtatcag
    18961 gcaagctaca ggtttcttag ctttaaaagc taaaatgacc ccttttgaag aattgatcag
    19021 tgaagaagaa ttgactggtg gggcgacaaa tgaagaatat aatcaaatgc aacagtatta
    19081 tatggattct tcccaaaatg cagcaatcga acaagcgtta aaacttgcag acaagcctta
    19141 taaaatggaa ttcaaaggcg tatatgtcat gagtgtagag aaaacatcga atttctttgg
    19201 caagctttca gttggagata cagtaacaaa agtagacggg aaaagctttc aaagtacaga
    19261 agcatttatg gattatgtaa aaagccaaaa agttggacaa acaatcgaaa tcagttattt
    19321 gcataatggt gaagaaaaaa cagcttctgg tgatctgatc gagttaccaa cagataaaaa
    19381 ggcaggaata ggtatcacgc ttactgacca taccgagatc gaatcagata cagatgttcg
    19441 tattaattct ggctctatcg gtgggccatc agcgggattg atgttcactt tagaaatcta
    19501 tgaacaagtc actggtaaaa acttgcgaca tggtaagcaa attgcaggaa ctggaacgat
    19561 caacagtgaa ggagaagttg gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga
    19621 taaagctggc gtagagattt tctttgcacc agatgatacg atttcagacg atgttaagaa
    19681 agaatatcct gaaataaaaa ccaactacga agaagcaaaa gctgccgcta aaaaattgaa
    19741 ttcctcaatg aaaatcgtac cagtaaaaac agtacaagaa gcgcttgact atctagagaa
    19801 aatgaaatga aactaaataa ttaaagaggg gttgtgacac gagt
//
LOCUS       DADXTG010000043        19802 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.044, whole genome shotgun sequence.
ACCESSION   DADXTG010000043 DADXTG010000000
VERSION     DADXTG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.044"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            241..516
                     /locus_tag="IWI57_001313"
     CDS             241..516
                     /locus_tag="IWI57_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449465.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            529..2601
                     /locus_tag="IWI57_001314"
     CDS             529..2601
                     /locus_tag="IWI57_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBC2449466.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2766..4652
                     /locus_tag="IWI57_001315"
     CDS             2766..4652
                     /locus_tag="IWI57_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2449467.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4664..5611
                     /locus_tag="IWI57_001316"
     CDS             4664..5611
                     /locus_tag="IWI57_001316"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBC2449468.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5631..6158
                     /locus_tag="IWI57_001317"
     CDS             5631..6158
                     /locus_tag="IWI57_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2449469.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6221..6874)
                     /locus_tag="IWI57_001318"
     CDS             complement(6221..6874)
                     /locus_tag="IWI57_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBC2449470.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7006..7848
                     /locus_tag="IWI57_001319"
     CDS             7006..7848
                     /locus_tag="IWI57_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBC2449471.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8006..8902
                     /locus_tag="IWI57_001320"
     CDS             8006..8902
                     /locus_tag="IWI57_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBC2449472.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8905..9618
                     /locus_tag="IWI57_001321"
     CDS             8905..9618
                     /locus_tag="IWI57_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBC2449473.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9638..10156
                     /gene="msrA"
                     /locus_tag="IWI57_001322"
     CDS             9638..10156
                     /gene="msrA"
                     /locus_tag="IWI57_001322"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBC2449474.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10153..10380
                     /locus_tag="IWI57_001323"
     CDS             10153..10380
                     /locus_tag="IWI57_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBC2449475.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10532..11083
                     /gene="lepB"
                     /locus_tag="IWI57_001324"
     CDS             10532..11083
                     /gene="lepB"
                     /locus_tag="IWI57_001324"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBC2449476.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11166..11666)
                     /locus_tag="IWI57_001325"
     CDS             complement(11166..11666)
                     /locus_tag="IWI57_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBC2449477.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11945..12343)
                     /locus_tag="IWI57_001326"
     CDS             complement(11945..12343)
                     /locus_tag="IWI57_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2449478.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12603..13463
                     /gene="ylqF"
                     /locus_tag="IWI57_001327"
     CDS             12603..13463
                     /gene="ylqF"
                     /locus_tag="IWI57_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBC2449479.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13456..14223
                     /locus_tag="IWI57_001328"
     CDS             13456..14223
                     /locus_tag="IWI57_001328"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBC2449480.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14279..15136
                     /gene="dprA"
                     /locus_tag="IWI57_001329"
     CDS             14279..15136
                     /gene="dprA"
                     /locus_tag="IWI57_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBC2449481.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15258..17336
                     /gene="topA"
                     /locus_tag="IWI57_001330"
     CDS             15258..17336
                     /gene="topA"
                     /locus_tag="IWI57_001330"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBC2449482.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17305..18681
                     /gene="trmFO"
                     /locus_tag="IWI57_001331"
     CDS             17305..18681
                     /gene="trmFO"
                     /locus_tag="IWI57_001331"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBC2449483.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18879..19784
                     /gene="xerC"
                     /locus_tag="IWI57_001332"
     CDS             18879..19784
                     /gene="xerC"
                     /locus_tag="IWI57_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBC2449484.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         6873 a         3408 c         4118 g         5403 t
ORIGIN      
        1 atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa catccttttg
       61 tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg gaatagaatg
      121 aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac aatgaaatat
      181 ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga gatctggaaa
      241 atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg cgccctttcg
      301 atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt gatgtatcgt
      361 cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt acagtgtgaa
      421 ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa gaaaaaagaa
      481 caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat ggaatttgct
      541 gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag ccgcattttg
      601 ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg tctgacagag
      661 tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat tgcaccattg
      721 ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt cgggacaatt
      781 ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc gacgttatca
      841 ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc gttaggtccg
      901 acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa aaaggcgatg
      961 catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc cttccagttt
     1021 gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat gagtctagta
     1081 gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa acaaaaaata
     1141 attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct gatcgaactt
     1201 ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg aatcattgct
     1261 gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt gtttgatgca
     1321 gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact gaatgcatta
     1381 gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg gggagacggt
     1441 ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat gctttttgtg
     1501 atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa aaagttcaaa
     1561 aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac agtcagtttg
     1621 gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca gcggtctttg
     1681 cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat cattgtcttg
     1741 cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt gatgatttta
     1801 gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa tacaaaagaa
     1861 tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga tctgtacttg
     1921 gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact gattttgtcg
     1981 atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc taatggttat
     2041 cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca gattttgtct
     2101 tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct ccatccgaaa
     2161 atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga agatatccaa
     2221 gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt agataacttg
     2281 actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa acttgaagga
     2341 ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga tagtctgttt
     2401 acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga atacgaagtt
     2461 gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa tttgttagaa
     2521 tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt tcgagcaaag
     2581 tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta taaaaaacaa
     2641 aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca aaaagaacaa
     2701 ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca agataaggag
     2761 ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt gaaaaagatt
     2821 tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg attgggacga
     2881 acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc agcgacggtg
     2941 atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct caagcagctg
     3001 aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc cccttgatcc
     3061 aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga gaagaccctg
     3121 atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat gcttggacta
     3181 cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta gataaaaaaa
     3241 tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg
     3301 aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa gcgatcgagt
     3361 ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc cacgatcgtt
     3421 attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa ttatacgagt
     3481 ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag
     3541 aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg agggctggcg
     3601 tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga
     3661 acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca caacgtttag
     3721 ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa acactgctag
     3781 aacatttaga tttattgatt caatctcggg aaagactagg tattactggt aaaaatggtg
     3841 cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat agcggtacaa
     3901 tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa gaaatggcac
     3961 cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa acagcagatg
     4021 gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga tttatgcatg
     4081 ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg ttgaaactgc
     4141 tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg gacatagcaa
     4201 cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc actgtttccc
     4261 atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa ggaaacggaa
     4321 aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa gagacccagc
     4381 agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa aagaagaaat
     4441 tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca gaacttgaag
     4501 aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc actcgattac
     4561 aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa atggcacggt
     4621 gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac aagcgtatct
     4681 tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa caggcacagg
     4741 cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt ttcctttgtt
     4801 aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt ttttaaaagg
     4861 agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg aatgggcatt
     4921 tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt ttggccatag
     4981 atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc ttttttgcga
     5041 gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac acatctacgg
     5101 ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt
     5161 gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg catggaatcc
     5221 ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat tttatgttca
     5281 agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc taggcgttcc
     5341 gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa caggtttagc
     5401 ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca
     5461 aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga
     5521 aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca
     5581 tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg
     5641 caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt
     5701 tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac
     5761 gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga
     5821 cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc
     5881 ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc
     5941 aagaatttct gccattttgt acgatattat accgtacagt catccatcat gtattcgatg
     6001 gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag
     6061 gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga
     6121 atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct
     6181 gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag
     6241 agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca tgcatatagc
     6301 ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg cctgctacca
     6361 tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc acgacacaga
     6421 gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc cgtttatgca
     6481 acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat acaaataaag
     6541 tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt cctgcaatca
     6601 aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt gtaaaaatca
     6661 atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca tagatagcat
     6721 aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga accactagtc
     6781 ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact tcatttaata
     6841 tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa
     6901 ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt acatgtagtt
     6961 taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac aaatgtaaaa
     7021 atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt
     7081 gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga ccaactagac
     7141 ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca
     7201 ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag tgaagtgatt
     7261 tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca agccagtaat
     7321 ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg attatctttt
     7381 caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa aatccttgaa
     7441 gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac actagataac
     7501 ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt tttgaacata
     7561 cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg gcgaggaaag
     7621 aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt gccaaatgtg
     7681 cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt caaagagggt
     7741 ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc ggttatcgct
     7801 acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc gaaaagagga
     7861 taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa attaggaaaa
     7921 taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat aacattagaa
     7981 ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga tcctcgtttt
     8041 gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta taccgaaagc
     8101 tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa aagaaaaact
     8161 tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa ccaagcaagg
     8221 aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga ccgcggttga
     8281 aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc gagtaaaaac
     8341 aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga ctgttggcgg
     8401 taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca attcctttaa
     8461 aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat ttcgatccct
     8521 caataaaacc gcacctattt acattttagg catctacaat ccattttatt tgaactttcc
     8581 tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag aaacggtgac
     8641 aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc aaggattgaa
     8701 tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga cagaaaatct
     8761 aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa ataatttagg
     8821 ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa accaatggtt
     8881 agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc aaacaaaaca
     8941 aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt acttggttcg
     9001 accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc agaaatacca
     9061 gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa agaaaaagtc
     9121 aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat tgaatataaa
     9181 ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg ttttcctatc
     9241 gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca actaaaagct
     9301 agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa tatgatcaag
     9361 agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac tatcatgctt
     9421 aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa aatcgactta
     9481 gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga tgcttcaaac
     9541 aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa agaaaataaa
     9601 aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa tatttgcagg
     9661 aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc atacagtgat
     9721 gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa aaagccaaac
     9781 gactggacat acagaagcag tggagattat ctatgatcca acattgattt catatgaaca
     9841 attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac aattcgagga
     9901 tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc ggatcattgc
     9961 tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa tcgttacaaa
    10021 aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt attataaaaa
    10081 gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg aagaaaattg
    10141 gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg aggcggtggc
    10201 acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca atttcctaag
    10261 cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga ctatttgccg
    10321 tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca tgacatataa
    10381 gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta attgaaagaa
    10441 ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata ataaagaaag
    10501 aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct atattaatcg
    10561 cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca tgttgcgggg
    10621 atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt tgaagcaagg
    10681 tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg ttgtttttca
    10741 attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag aaaatgtcag
    10801 ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt atctaactaa
    10861 aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac tacaagagct
    10921 aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata atcgaagagt
    10981 ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag gtaaagcaag
    11041 atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa taaagcgtgc
    11101 agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta ccttagtacg
    11161 taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat aaatgaataa
    11221 acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc tagaaaaatt
    11281 actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa aaggctgact
    11341 gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt tttatgatat
    11401 aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc agcttgaaac
    11461 gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag caaaccagcc
    11521 cagatcggtc catatagata acccataagc gccatactta aaaatccgaa tgtgatttga
    11581 aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa taaaagagca
    11641 gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt tgcaatcaaa
    11701 gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata aaatcaccaa
    11761 tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa aaaagggtga
    11821 gaagactgct tttataaact gtaaaaaaga gattgtacga ataaaaaaaa tagaggcgga
    11881 gaatacttat cgtatcatcc gcctttgttt ttttgttata aaaaacgtca gattagttat
    11941 ctgcttacat cttcatccat acgggttcta atgtatttcc gtctagatct tggacttcta
    12001 atgaaaacat ttcttcttca ggcattccca tatctacttc gtggaaagta ccaccatttt
    12061 cttttgcaat ttgagcaaat ttttttactt catccgcgtt ttcaacacta aacgaaatca
    12121 aagcaccact tgttttttta ttatcagcta tttctttatc ttttataaat tttttataaa
    12181 aatcatgaga gagaagcatt atccaaaaag tgtcatccca taccattgaa ccagcttctt
    12241 cagtagaaaa ttcttcattt tttttaaaac ccagcttttc ataaaaagca acggaacgat
    12301 tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt catttgtttc gtctcctttc
    12361 tgttttgttc gtctatcatc tcttatttag gaaacgctgt caaaaattta gattcatata
    12421 tagaaaccaa aataaaaatt tgcatcatca aaaaagtgaa gatgtgatag aatgaggact
    12481 ggttcacttt cacttaacta atagatggaa tttctttata ataaaaaagt gaatttatat
    12541 tgaaaaagaa aaaaacaagt aaagccgggg aatattccgg tatgaaagga atggagagaa
    12601 caatgaccat ccaatggttt ccgggacata tggcaaaagc ccgacgagaa gtttctgaaa
    12661 aaatgaaata tgtggacatc gtatttgaat tggtagacgc acgattacct ttatcttctc
    12721 gtaatccaat gctagatcag atcattcagc aaaaacctcg ccttgtttta ttgaataaag
    12781 cggatctggc agatccacaa cagacaaagc tgtggcaaga atattttcgt aaacaaggcc
    12841 atcttgcatt agctatcacg gcacaagaaa gtaaaggaat taaaggtcta gtacctgcgg
    12901 ctaaagaagc attgaaagaa aaacgggcaa gagatgctgc tcgggggatc aagcctcgcg
    12961 ctattcgggc aatggtctta ggtattccaa atgttgggaa atctacgttg atgaatcgac
    13021 tagtaggcaa gaaaattgca cagacaggaa ataagcctgg cgtaacaaaa ggtcagcagt
    13081 ggcttcgttt aggtaatgag ttggaattgt tagacacacc agggatttta tggcccaaat
    13141 ttgaagatga agaaatcggc aaaaaactag cgctgacagg cgcgatcaaa gaccagcttg
    13201 tccatttaga tgatttagcg atttacggat tagacttttt tgcccgatat tattcaggaa
    13261 gaatcagcga tcgttatggt ttatcaatcg agcaagaaga aaaattggca ccggaattac
    13321 tgatggatat tacagcaaaa cgtgggtata aagaagacta tgatcgtggc agcgaattag
    13381 tcgtgcatga tattcgtcaa ggaaaacttg gacgatacac actggatcgt tgcgaagaaa
    13441 tggagcaaaa aagtgatgac tgaatccatc caaagtatca aagaacgatt gaaaagcata
    13501 cagagaatag atgatcctac actagaatca ttaagaaatg atccacgcaa aggcgtgcag
    13561 caagcactgg cttcttttga acgacgattg aaaaaggaag cattaattaa ggaaaaatat
    13621 gaagaaatgt tttgctttga aaagcaaggg tacgcacaag gtcaccaatg gattgccggc
    13681 attgatgaag taggaagagg accattagct ggacctgtcg tggcagctgc agttatcttg
    13741 ccgaaaaatg agatcatatt agggctgaat gactccaaac aattatcaga gaaaaagcga
    13801 gaacttcttt atcaacaagt tcaagaaaaa gccctggcaa tcgggatcgg gatcgttgat
    13861 cagcagacaa tcgatgatgt gaatatttat gaagcaagta aattagcgat gatccaagca
    13921 gtagaaaaat tgtccattgt accaagctat ttgttgatcg atgcgatgac cttggatttg
    13981 cctattcagc aggaaaaaat aatcaaaggg gatgcacgat ctgtgtcgat tgctgctgca
    14041 agtatcattg ctaaagtata tcgagaccaa ttgatgaaag aatatgacgt gctttatcct
    14101 ggatatggtt tctcgcataa tgcaggttat ggaacaaagg aacacttaga aggacttgaa
    14161 cgacaaggaa ttacgccaat ccatcgtaaa agttttgcac ctgtaaaaaa atatttacaa
    14221 taaaaaacag ccgcttttac gtatcttatt agaaagagag acgaaaggcg gttttatgat
    14281 gtatcaaata gaagaaaatt tattgaaact tgcttataca aaaggaatca gtagtcaagg
    14341 aaaatgggca cttgctaact ggatgatgca gtatccttat aaagaaatcg gatttgaaga
    14401 catcattaag ataggtaaaa taaccaaaca tcgggagctg tttcgcaaaa gttggcagga
    14461 aattcacgct aactggggca aaatccaatc gaaacagtcg tttatcactt gctttgatcc
    14521 caaatatccc cctcagctgc ttcacttgac ctatccgcca attgtactgt tttataatgg
    14581 tgatctatct ttactttctt gtaacatgtt aagcgttgtt ggtggaagac acacttctgc
    14641 tttagccaaa aaaacagttt attatttact aaaaccagtg atagaagctg gatatgtgat
    14701 cgtcagtggc tgcgcgaaag ggatagatac atacggtcac caagcagcaa tctctcatgg
    14761 tggacggaca atcgcagtca ttggaacagg gatagagcaa gtctatcctg ctgaaaatcg
    14821 tgacttgcaa gaggtgattg caaatgatca tcttcttttg agtgaatatc ctcctcaatc
    14881 cggcccgaaa aagcattgtt ttccaatgag aaatcggatt atcgcagctt tgtctaaagg
    14941 aacatgtgtc tttgaagcca agaaaaaaag cggctctctg ataacagcac agcaagcatt
    15001 ggatcttgga agaacagttt tttctgttcc aggaaatatt ttaacagaac attctaccgg
    15061 atgtcatcat ttgatccaag acggtgcagt ttgcacggtt tcgggtgaag atatcttggc
    15121 agaatttaaa gaataacacc atacttttca tttgcttttc ttgacaaacg ttttctgaat
    15181 ggatatgatt agctacgatt tgtatgaatc ctcttttaat agtaggaaca cgacaatgga
    15241 aaggagtcca ttcagtaatg gcgtataaat atttagtgat tgtagaatca cctgcaaaag
    15301 ccaagacaat tgagaaatat cttggacgaa actataaagt aatggccagt gttggccata
    15361 taagagattt accgaaaagt aaaatgggta tcgattttga aaacaattat gaaccccatt
    15421 atatttctat acgcggaaaa ggcgatgtca tcaaaagcct gaaagccgca gcaaaaaaag
    15481 ctcaaaaagt ttacttggca agtgacccgg atagagaagg agaagcgatt gcttggcatt
    15541 tagcgtacct acttgggttg gatctgaaag aaaaaaatcg ggtggtcttc aatgaaatca
    15601 caaaagacgc agtcaaagca gcttttaagg aaccaagaac gatcgatgta gatttagtag
    15661 atgcacagca agctcgtcgt accttagaca gaatcgttgg ttattcgatc agtcctattc
    15721 tctggcgtaa ggtcaagaaa gggttaagtg caggacgtgt ccaatctgtc gctttaaaaa
    15781 ttattattga ccgtgaaaaa gagatccgag aatttgttcc agaagaatat tggagcatcg
    15841 acggtaattt taaaaaagct cgcaagaaat tcaaagcaaa tttctgggga atcgacggta
    15901 agaaaaagaa attaccagat gcacaaagtg taaaagaagt cactgctaga attgatggaa
    15961 cagacttcga agttaaaaaa gtagaaaaga aagagcgcaa aagaaaccct gcgaatcctt
    16021 tcactacaag tagcctgcaa caagaagctg cccgtaagtt aaactttcga acaagaaaaa
    16081 ccatgatggt agcacagcaa ttatatgaag gaatcacttt gggtaaacaa ggaacagtcg
    16141 gcttgattac atacatgcgt actgattcta cccggattgc cgatacagca aaagcagagg
    16201 ccgcagcatt tattgaagat acgtatggaa aagaattttc tagacatgat caccgaaaga
    16261 tcaaaaatgc tcaaggcgca caagatgccc atgaagctgt gcgtccaacg agtgtgcttc
    16321 gtacaccaga tgaactcaaa caatatttag ataaagatca attgaaactt tatacattga
    16381 tctggtcacg ttttgtggct agtcagatga caccagctat tttagataca atgaaagtga
    16441 cattagaaca aaatggtgtc atcttcattg cgaacggttc aaaaatcaaa ttcaaaggat
    16501 tcatgcaagt gtatgtggaa ggtagagatg acggcaaaga agagaaagaa aatattcttc
    16561 cagaacttga agaaggcgat gtagtccaat ctgttgatat tgaaccaaaa caacatttta
    16621 ctcaaccacc agcacgtttt agtgaggcta cgttgattcg tgcactagaa gaaaacgggg
    16681 tagggcgacc ttccacttat gcaccaacac ttgatacgat ccaaagacgc tactatgtaa
    16741 aattgacaca aaaaagattt gagccgactg aactaggaga aattgtcaat tcattaatct
    16801 gtgaattctt ccctcaaatc gtcgatatcc atttcacagc agaaatggaa ggagatttgg
    16861 ataaaattga agaaggcacg gaagcgtggg tcaaagtggt tgatcgtttc tataaaccgt
    16921 ttgaaaaaga actgacgaat gcggaagaaa aaatcgaaaa aattcaaatc aaagatgaac
    16981 cagcaggatt cgattgtgat gtctgtggac atccgatggt gataaaatta gggaaatatg
    17041 ggaagtttta tgcttgcagt aattttcctg attgtcggaa tacgaaacca atcgtaaaag
    17101 aaatcggtgt gacttgccca gtatgtcatg aaggacaagt cattgaacga aaatctaaga
    17161 aaaacagact attctatggt tgttcccgtt tcccagaatg tgaattcact tcttgggata
    17221 aaccagtggg cagaaattgt ccaaaatgtg acggatatct ggtcgagaag aaagtcaaag
    17281 gcggtaaaca agtagtctgt gtaaatggag attacgaaga agacatccaa aaataataga
    17341 aaagaggttc tttcattgaa aatggtaaat gttgttggag cagggcttgc cggaagtgaa
    17401 gctgcttggc agatcgcaca agcaggagtc cctgtccgtt tatatgaaat gcgtccagtc
    17461 aaatcgactg aggcgcatca cacaagtaat tttgctgaac tagtatgttc caattcatta
    17521 agaggaaaca gtttggctaa tgcagtaggg ttattgaaag aagaaatgcg ccgattgaat
    17581 tcggtagtaa tcacttcggc agatgaaaca gcagtgccag ctggtggtgc cttagctgtt
    17641 gaccgcgatt ctttttctga aacaattacc cgaaaagtca aagagcatcc attagtaaca
    17701 gtgataaacg aagaaattac tgaaataccg gaaggaatca ccgtagtagc aacaggacct
    17761 ttgacttctc atccattagc agaaagcatc aaagcattca atggttcaga tggtttttat
    17821 ttctatgatg cagctgcacc aatcgtagac aaagctacga tcgatatgga taaagtatat
    17881 ttgaagtccc gctatgacaa aggagaagca gcttacttga actgtccgat gacagaagag
    17941 gaattcaaag ctttccatga ggcactaatc aatgccgaag tggcagaact taaatctttt
    18001 gagaaagaaa aatattttga aggatgtatg ccaattgaag taatggcaca gcgaggattc
    18061 aaaacgatgc ttttcggacc tatgaaacca gttggattgg aagatcctaa aactggcaaa
    18121 cgtccttatg ctgttatcca gctgcgtcaa gacaatgctg cagcttcttt atacaacatc
    18181 gtgggctttc agactcactt gaaatggggc gagcaaaaac gtgttttccg gatgattcct
    18241 gggttagaaa atgctgaatt cgttcgctat ggtgtgatgc accgaaacag ctttatgaat
    18301 tcgccagaat tgctgaaacc gacttaccaa tcaacgaaac gcgaagatct gttctttgca
    18361 ggtcagatga ctggtgtaga aggctatgtt gaaagtgcag cgagcggact acttgctgga
    18421 atcaacgccg cacgcttagc aaaagaaatg gaaccagtcg agtttccaca agaaacagct
    18481 attggaagta tggcttacta cattactcat gcggaaggaa aacatttcca accgatgaat
    18541 gccaactatg gtctgtttcc tgaactgcca gagcgcattc gagacaaaaa agcaagatat
    18601 gaagcaattg cacaacgagc tttaaaagct aatgaggcaa taaaaacaga attagaaaaa
    18661 actgcaatcc caaatgagta aggaaactcc atgaagaggg tgagacggat agtctcgctc
    18721 tttttctggc gtttttctta agaatttctc aaacaaaatg tgaggaattg cgccaattgt
    18781 gaacaaactt agaaatattg gttaaattct gacaatttat ttgtcttttt ataaacgctg
    18841 tgctaaatta ttctctggga caaaaggaag gtgagaaaat gacagaagcc acgtgggaac
    18901 aaaaattttt gcgttatttg attgtcgaaa gaggatattc agaaaaaacg aaagaagcat
    18961 atgaagaaga cattctccat ttcaaacatt ttttggagga atctggagac gctgatctgc
    19021 tgaaggcgga atattttacg gttcgtgtct atttgagtgc attagctgat catcgtccta
    19081 gctatagcag aaattctatc agtcgcaaga tagccagcct acgttcgttt tatcagtatc
    19141 ttctcaaaga agaagtgata aaagaaaatc cattttctta cgttcatctc aaaaagaaaa
    19201 atttgagact gccacgtttt ttttatgaaa atgaaatgca agcacttttt gacagcgtag
    19261 caggagatag tcctttggat ttgaggaacc gcgctttact ggaagtcctt tacgggtcag
    19321 gcatccgttt aagtgagtgt agcagtttgt cggtatcaga cattgatttt gattcagagg
    19381 tcatgttgat ccatggtaaa gggaacaaag agagatatgc tcctctaggt tcttttgctc
    19441 aagatgctct gcaagaatat ttcagggaag gccgccaggt attgatgaca aaatatcacg
    19501 aagagcatga tatcgttttt gtgaatcatc atggaaaacc aatcacgcct actggaattg
    19561 aatatgtctt gaatcaaatg atcaaaaaaa gcagtttgga tagtgatatc catcctcaca
    19621 tgctgcgcca tacatttgct actcatttat taaataatgg ggcagatttg cggacagtcc
    19681 aagaattgct aggacatgcc aatttatcga ctacccagat ctatgcccat gtaacaaaag
    19741 aaagtttaca aaaaaactat cgttctttcc atccaagagc gtaaaaataa ggaaaagaac
    19801 aa
//
LOCUS       DADXTG010000044        19707 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.045, whole genome shotgun sequence.
ACCESSION   DADXTG010000044 DADXTG010000000
VERSION     DADXTG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.045"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(308..1333)
                     /locus_tag="IWI57_001333"
     CDS             complement(308..1333)
                     /locus_tag="IWI57_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2449485.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1608..3293
                     /locus_tag="IWI57_001334"
     CDS             1608..3293
                     /locus_tag="IWI57_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBC2449486.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3307..4590
                     /locus_tag="IWI57_001335"
     CDS             3307..4590
                     /locus_tag="IWI57_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBC2449487.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4660..5529
                     /locus_tag="IWI57_001336"
     CDS             4660..5529
                     /locus_tag="IWI57_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2449488.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5526..6362
                     /locus_tag="IWI57_001337"
     CDS             5526..6362
                     /locus_tag="IWI57_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2449489.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6388..7455
                     /locus_tag="IWI57_001338"
     CDS             6388..7455
                     /locus_tag="IWI57_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBC2449490.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7452..8279
                     /locus_tag="IWI57_001339"
     CDS             7452..8279
                     /locus_tag="IWI57_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBC2449491.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8324..9349
                     /locus_tag="IWI57_001340"
     CDS             8324..9349
                     /locus_tag="IWI57_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2449492.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9346..11529
                     /locus_tag="IWI57_001341"
     CDS             9346..11529
                     /locus_tag="IWI57_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBC2449493.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11529..12659
                     /locus_tag="IWI57_001342"
     CDS             11529..12659
                     /locus_tag="IWI57_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBC2449494.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12656..13333
                     /gene="pgmB"
                     /locus_tag="IWI57_001343"
     CDS             12656..13333
                     /gene="pgmB"
                     /locus_tag="IWI57_001343"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBC2449495.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13417..13944
                     /gene="msrA"
                     /locus_tag="IWI57_001344"
     CDS             13417..13944
                     /gene="msrA"
                     /locus_tag="IWI57_001344"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBC2449496.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14145..14831)
                     /locus_tag="IWI57_001345"
     CDS             complement(14145..14831)
                     /locus_tag="IWI57_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBC2449497.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14911..16449
                     /locus_tag="IWI57_001346"
     CDS             14911..16449
                     /locus_tag="IWI57_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBC2449498.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16569..17036
                     /locus_tag="IWI57_001347"
     CDS             16569..17036
                     /locus_tag="IWI57_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBC2449499.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17555..17998
                     /gene="rplM"
                     /locus_tag="IWI57_001348"
     CDS             17555..17998
                     /gene="rplM"
                     /locus_tag="IWI57_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBC2449500.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18014..18406
                     /gene="rpsI"
                     /locus_tag="IWI57_001349"
     CDS             18014..18406
                     /gene="rpsI"
                     /locus_tag="IWI57_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBC2449501.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18494..19681)
                     /locus_tag="IWI57_001350"
     CDS             complement(18494..19681)
                     /locus_tag="IWI57_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2449502.1"
                     /translation="MRYPKRFKEITGIASEKYQKTFPNRQLAIKAENDMKKKIEKVLR
                     EENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITISNTKDIFRLHILPM
                     FGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRTTKI
                     IQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLY
                     LGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSE
                     WKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHL
                     SPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLN
                     KCD"
BASE COUNT         6343 a         3562 c         4154 g         5648 t
ORIGIN      
        1 tgtctgttat tctatttatg ttttagagat aagtatcccc aaataacatg gattgaaata
       61 agggtaaatg tgtttaaaaa aacgaactat aaaaacttat acacaacgtg gttaaaaata
      121 gttttttctt ccctgtggaa aaaatagttt aacacaaaca ttatagttat ccacagctat
      181 ctaaaacaga tggaaaataa gaaaaattcg ctaaaatacc gggagggggt aacgagggat
      241 gagtgattga aaaaagaaca gagtaactga cttatcctca gtgtttctgt tcttttataa
      301 ttctgttcta catttcttgt tgttggatcg aatctgtgag agtaatctcg gttgaaagaa
      361 tgattctttt ttcttcaggg tattggtggt ggattagttg gaataaaagt tctgtggctt
      421 tgattcctaa ctgatttgtc ggaatgtcta tccgagtcaa ctgtggcgaa aatagggagg
      481 acaataaggg atcactgaat cctgcgatcg aaagatcatc aggtatctgc tttccagatt
      541 gttctgccac tttgtaaata ctcaatgatt ttaatgcatc ggtagaaaaa atcgcagttg
      601 gtctttctcg tagcttcatt aaatcattca ttgctgccat gctatcttct aagcgaaagc
      661 cgccgtctat aatccattca ttacggatat ctaggtggtg atcaaataag cattgacggt
      721 aaccattgag acgatcattg gatacataaa cttctgtcgg agcatggaga cagccgatat
      781 gacggtgtcc attcttgatc agtgtttcca ccaaatcgta actatcctgg aaattatccg
      841 tgtccactga gtagatgttc gtgtagtcgt ttccgacttt tccgattacc acaataggaa
      901 tattgtattg atccaattca aaaagaagtg tttcatccat cgtagagctt aaaataataa
      961 ttcctcggat cattttttct tggatctttt tcgcgcattt ttctagctct tcttctggtg
     1021 tttttgccgt ttgtagaatc aagtcgaagt tattttgttc agaaacggca gaaatggatt
     1081 gaataacatc tgaaaaaaaa ggattgccat aggtcgtatg agaagtacga tgagataatg
     1141 ctagaatcgc gtcaaaacca tttcctgaca gtcctcttgc aattttgctc ggttcataat
     1201 tcaattcttt gattgcttga agtacacttg cttttgcttt atcagagata tttggctggt
     1261 tatttaatac tctggagaca gttgattttg agacattagc ttttttcgca atgtcataaa
     1321 tagtaggttt caattttata tgcacctcct ttttgggagc gttttagaat aattccatag
     1381 tacaatagac attctttttt gtctatgatt ttgaaaattt aggaatagct tgcatgatca
     1441 tgagagacca tattttttta agaagctatt aattattgat acataagctt tcttatttgt
     1501 atttttgtgt atatcaaaat tatttaaaaa aatgctattg acagtaagcg ttttccataa
     1561 tatgatcaaa tcgttgggaa cgctcccaaa aaagaaaggg gaatccgatg tatacaaaac
     1621 ttttacaagc gatttatcgc gactcagatt tgtctgagaa aaccgcctct atgattgaac
     1681 aaaaagtcag tgtttatcag cagaagaaag gccaggcagg caaactgcat tgggacgaag
     1741 aagatatttt cttgattact tatggggatc aattttttga agagcagcaa ccaactttgc
     1801 gtacatttaa gaaattttat caaaagtatt tggcagatac atttagagtt gtacatttct
     1861 tgccattttt cccatataca tctgatgatg gtttctccgt tgtaaattat caggcaatca
     1921 acgaccgttt gggcacatgg gaagatatag aagaaatgag aagcgaaaca cggttaatgt
     1981 tcgactttgt ctgcaatcat atgtctgccg aaagtacttg gtttcaggaa tatttaaact
     2041 tgaatgatac gtacaaagat ttttttattg atttgccact ggatacagat acaagcagcg
     2101 tgactagacc aaggaccagc ccgttactta caacgttcat tgacaaaaac gggaaagaaa
     2161 aacacgtatg gacgactttt agcgaagatc aagtagactt gaattttaaa aatccagaag
     2221 ttttattgaa aatgatcgat attcttttgt tttacttaga aaaaggcgct tcatttattc
     2281 gcttggacgc tgtgggattt ttatggaaag aactaggaac ttcttgtata cacctgccag
     2341 aaacgcataa aatcatccaa ttattccgtg caatcgctga tgaagcagct ccaggaacgg
     2401 tattgatcac tgagacaaat gtgccacaca aagataatat cagttatttt gggaatggcg
     2461 agaatgaagc acagatggtc tatcagtttt cgctaccacc tttgaccctt caagcgattc
     2521 gttcgggtaa tacaagtaag ttgaaagaat ggttaaaaac catcgatttc gcatcgaaaa
     2581 aaacaacttt ttttaatttt ttagcctccc atgacggtat cggattgaat ccggtacgag
     2641 gtattctgcc ggaaaaaacg atccttgaat tagtggaaca gctgaagaaa gagggtaccc
     2701 gagtaaacta caagaaaaac ccagatggaa cgaccagtcc atatgagata aacgctacgt
     2761 atctagatgc gttaagttct ccaacagata cgacggaatg gagaagcaaa cgctttttag
     2821 ctgctcattc atttttggta acgttaccag gcgtgccggc aatttatatt caaagtgtgc
     2881 tgggaagtcg aaatgattac aaaggcgtag aaaaaacagg agagaatcga aaaatcaatc
     2941 ggcaaaaatt tttgttagca gatattgaga aagagttgca gacagatcca ttaagaacag
     3001 cagtatatgg tgaattgacc cagatgctat ctattcgaaa aaaagaaaaa gccttccatc
     3061 cgaatagttt gatttactta ctggactcag cagattcttt tatcagtttt attcgcgaag
     3121 cgaaggaaga gaaagtctta gttatccata atttttccaa tcaatcagca ggttatcttt
     3181 taccaaaagg agattaccta gacctgtttt ctggacaaat tatccattgc agtcaagaac
     3241 gaaagttgat attccagccg tatgaatata tgtggctgaa aataataaaa taagaggtgg
     3301 aaaaaaatga aaaaaattat tgttactacc tgtctgctag ctactggatt tcttcttact
     3361 gcttgtggtg aaagtcaaag ctctaacgaa tcacaacaga caacgattca attcatgcac
     3421 tcttctgtcg aacaggaacg attaagtgtg atcaatgaac tggttgctga ttttgaaaaa
     3481 gaaaatccag acatcaaaat caagcaagtg ccggtagaag aatcagcgtt caatacaaaa
     3541 atcgttacgt tagctcgttc cggtaaatta ccagaagtaa tggaagtcag tcaggatttt
     3601 gctaaagtga tggataaaga tgaactgatt gatcagacgg cagtccaaaa cgttatcgag
     3661 gaagctggag aagataatta ttatgacgga gccaaaaacc ttgttcgttc agaagatgga
     3721 aaaagctata ttgctgcacc aattagcggc tgggttcaag gtatttggta tgacaaagaa
     3781 aaattatcag aagctggttt ttcagaacca gaaaattggg atgatatttt aaaaattgct
     3841 caacatttca cagataaaga gaataaacaa tatggaattg ctatgcctac tgctgaagga
     3901 acaatgagtg agcaagcgtt ctctcaattt gctttgtcaa atggagcaaa tgttttggat
     3961 gatgaaggcg aggtaacgat cgatacggaa aagatgagag aagcattgtc gttttatcaa
     4021 aacttatccc aatacacgat gccaggctct aatgatgtga cagaaatcaa agatgctttc
     4081 atgaatggaa cagcaccaat ggcaatctat tcaacttaca ttttgccttc tgtttatgaa
     4141 gcggggaact cagaaaatat cggttttgct atcccaacac aaaaagatca agctgtctat
     4201 ggcacagtat ctggattgac gatttcttct ggtctagacg agaaacaaaa agaggctgct
     4261 gaagcattca cagctttctt atctcaaccg aaaaacatgg aaaagtgggt cttgatgtct
     4321 cctggtggtg ctcaaccagt gaataaacaa gtagtcgaac tggatggtta caaagaaaat
     4381 gaagtcatca agtcttttgg cgagctgcca tctgagattg ctagtgcttt tgatgaagta
     4441 caagtctttg gactagtcgg ggaaaagaat ttcacgaaaa tgggagatat cacaagctca
     4501 ggtgtgatcg gaaaagcagt gaaccaagta actgtcggaa acgaagatgt cgatcaagct
     4561 ttagctgacg cacaaaaaaa tacgaaataa caagaagttc tgccaatggt gttcgaaaaa
     4621 agtagatagc tattggcaga ttatcagggg gaggtttcaa tgaatcgtcc aaggaaacat
     4681 tctgacttaa aattagccat gggactttta gcaccaagca tgcttttatt agctgtttta
     4741 gtgctgattc caatgctttc caatattcag atcagttttt tcgatatgcc gctgaatcct
     4801 aactcagacg ccgtttttat cggattaagc aattatcttg aaattttaag tgatagtgaa
     4861 ttttatcgtt ctttaggttt aacagttgct tacacattct tagttgtagc aggcagtaca
     4921 gctttaggat tagccgtagc tattttgctt aaccgcaagt tcagatttag gaaaacggtg
     4981 cgttcactga ttttattaag ttatgttacg ccatctattt ctttgatttt tgcttggaaa
     5041 tatatgttca ataatagtta tggaatcgtc aatgatctgt tagtagatag gttgcagctt
     5101 ttcaacgaag caccgctgtg gttcgaccag ccagttgcta gcttctttat ggtcgttttg
     5161 tttgctatct ggagatacta tccgtatgcg tttatttcat ttttggctat tttgcaaaca
     5221 gtcgacaaaa cgctttatga agcagctgaa atggacggtg caaatgcttg gcagaagttc
     5281 aagatcgtga cactgccggc aatcaaacca gtactagcaa ctgtgattgt tttacgatct
     5341 atctggatgt tctatatgtt tacagatgtc tatctactga cgaataaagt caatatctta
     5401 ggcgtttatt tgtatcagac agcctttgcg ttcaatgatc ttggaaaagc agcagctata
     5461 tcgattctat tgttcatcat tattgcagcg gtcgtcttgc tctcacgaaa gaaggtggac
     5521 ttgaaatgaa aaataaaaag agcaggaaaa tcttatttta cgcgacgatt ttgttgctat
     5581 tagctgtcaa tttgtttcca tttttgatca tgctgatgac atcattcaag gattcacaag
     5641 aagcaattgc aacgacacaa actatttttc cgaaagaatg gacttttgaa cattatcagg
     5701 atattttcaa tgctgagatt tttccattca tcatgtattt caaaaatagt ttagtgatct
     5761 ccttgatagc ctccgtaact gcagtagtga ttgggatcag cggggcatac gctttgtcaa
     5821 aactacgctt caaagctcgc tcgaaaatca atgctagctt ttacaccgtt tatatgtttt
     5881 ccggtatttt gatcgttgtt ccattattta aaatcatttc cggtctaggt ttgtatgata
     5941 ccaatacagc actgattatc acgatggtcg ttcaaacact tcctactgcg atattcatgc
     6001 taaaaagtta ttttgacacg attccgactg atattgaaga agcagcaatg atcgatggtc
     6061 ttagccggat ccagatcatt attcggatca ttataccatt ggctatttct ggaatcattt
     6121 ctgtcttcgt ttattgcttt atggtttcgt ggaatgatta tttatttgct tcgattttcc
     6181 tgtcgacctc cgaaaaattt actttaccaa tcggtttgaa cacattattc agtacaccag
     6241 actacatttg gggacgaatg atggccgctt cattggtgac cgctttacca gtcatcatta
     6301 tgtatgcgtt atctgaacga tttattaaag gtgatttagt cgaaggcggc gtaaaaggat
     6361 aaaaagaaaa gaaagaggaa agaaaagatg aaaaaattag ttgcattaga accaagaata
     6421 gccggattca aagattacga agaccgtgag atcgaaaaga acgaagtacg tatccgagta
     6481 attttcgcta ctccaaaacc aggaacagaa attgtcgact ttagaggatt aagtccattt
     6541 atcgatgaag aattttcaga agaatggcgc ctattcttgc caagagaaga aggaagccaa
     6601 cgcggtgtgg tatttggcga atttcaatta ggaaatatga ttgtcggtac gatcgaagaa
     6661 acgggcagtt tggtgacaga gtatcaaaaa ggagagttag tctgcactta tggacccatt
     6721 cgtgaaacgc tgattacaaa cggtgttgat aattataaac tacgtaaact tgatcggttg
     6781 gatcaatgga aaaatgcagt atgttacgat cctgctcaat tcgctttagg tggtatccgg
     6841 gaagcacatg ttcgagcagg tgatgctgta gcaatcttcg gtttaggcgc tatcgggcaa
     6901 attgcgattc aattagctaa aaaagcaggt gctactacca tcgttgggat cgatcccata
     6961 gcgcagcgta gagaattagc agaaaaatac ggtgccgatt acacgctaga tcctatgaaa
     7021 gcagatgtcg gttttcgtct aaaggaattg acagataaac gtggagtaga ttcagttatt
     7081 gaaacgagtg gcagtgctgc cgcattacaa gcttctttaa gagggatcgg ctatggcggg
     7141 atcatttctt atgttgcctt tgctaaacct tttccagctg ggtttaatct tggtcgcgaa
     7201 gcacatttca ataatgcaaa aatcgtcttt gctcgagcat ctagtgaacc aaacccagat
     7261 tacccaagat ggaaccgtaa acgaatcgag gacacatgtt gggaattatt gatgaacggt
     7321 tacttggatt gtgaagaaat cattgatcca gttgtgccat ttgcagaaag cgcagaagct
     7381 tacatgcgtt atgtagacca gcacccagaa gaaagtatca aaatgggcgt tcaagtccga
     7441 ggaggagaac aatgaaattc ggtacgcaag atcaagcatt tttcccaaga gacattttag
     7501 aaaaatttag atttatcaaa gactgtggat ttgaagaata cgaaatcgac ggtaagttgt
     7561 taatagaaaa tatcgacaca gtcaaacagg caatcaaaga aacagggatc ccagtagtga
     7621 cagcttgtaa cggctacgat gggtggatcg gtgactttat cgaagaacgg cgtcttaatg
     7681 gtgtacgtca aattgctgag attttgcgtg cactaagcga ggttggaggc aaaggaatcg
     7741 ttatcccagc agcttgggga atgttcactt atcgattacc gccgatggtt tcaccccgaa
     7801 gcaaagaagg agactataaa gcagtcagcg aatcacttcg ttatttagat aaagtagccg
     7861 aagaaacagg tacggttatt tatttggagc ctttgaaccg ataccaagac catatgatca
     7921 atttgttagc ggatgcgcgt cattacattg actctaatca attgaaacat gtgcagatca
     7981 tcgctgattt ttatcatatg aatattgaag aagatgatat ttcaaaagca ttgactacgc
     8041 aaaaagagta tgtgggtcat atccatttag cagataatca tcgctaccaa ccaggatctg
     8101 gcgcaattga ttttactcgc cattttcaaa cgctacttca aaatgagtat gaaggttata
     8161 tggtatatga atgtcgtttg cgaggagaaa atgaagtgaa aatttatcag gaatcattgc
     8221 aatacttgaa aaactgtctg gaaacagcgc gtcatttaaa tgatttagag aaagaataga
     8281 gaaagaataa caataagtaa ataagcaaag gagggagaaa taaatgaaga aagtagcaat
     8341 cataggcgct ggacaagtag cagaaaaagt acatgctgct tattatcaaa aaagatcaga
     8401 tgtcttagaa ttggccgctg tggttgatcc agtcagccag cgagcagaag acttttgccg
     8461 acgtacagga ttttctcatg cgtatacatc agttacacaa atgctgaatg agatttctcc
     8521 cgatatcgtg agtgtctgta cacccaatcg ctttcacttc gaacatgtat tggctgccct
     8581 cgaagctaat gcttcagtga tgtgcgagaa accaccagct atgacagctg atcaagcaag
     8641 aaagatggca gagacagccg ataaaaacgg gcagattttg gcttatggat tccagcatcg
     8701 tttttctgac gaagcaacaa tgatcaagaa tcatcttgat gttctcgggg atatttatta
     8761 cgtagaagcg aatgcgcttc gtcggtctgg tgttccaggc tggggaacgt ttatcgacaa
     8821 agaacttcaa ggaggcgggc ctttgatcga ttatggcatt cacatgcttg atacagcgat
     8881 gtatattact ggatttccta aagtaaaaag cgttcgggcg tacagcttcc aaaaaattgg
     8941 tacgaaaaaa aattcaggga aattcgggaa atgggatcca agcaaattca cagtagaaga
     9001 tgcaattttt ggcacactgg aattagaaaa cggcaagatc atccaactga atacttcgtt
     9061 tgctttgaat atcagagaag acaaactgaa tgtggctttt tgtggcgatc aggcaggtgc
     9121 taatctgtat ccaggcgaac tattcgaaga tgtagatgga aagctggttt ccttacaaaa
     9181 gaccacgcca gttaatgacc cacatgaact aagtatggct cattttgtga atcgggcgct
     9241 cggtgaccag actcagatga ttgcagatgg ctggcaagga tatgtcatcc agaatctggt
     9301 ggaaaattta tatcgttcgg cagaaacagg agaagttatt accttatgaa agtagaagaa
     9361 aaacagtttg atttagagta tatcaataag tatgcgagtc agatgaccat tggaaatgga
     9421 ttttttggta tcagaggaag ccaggaggaa gactatcgga atcaagtaag gggaatgttt
     9481 gcagctggtg tatataaccg acctttagga gcagaatcag ccgagttagt gaatctccca
     9541 gatgtcatca gatgggaaat aaaaatcgac ggtgaacatt tttctcttga gacgatgaaa
     9601 gttcttgcat acgagcgggc actagattta gaaatgggag agcttaaacg ctatgcgctc
     9661 attgaaactg ctcaaggaaa aaaagtggaa gtcattactt gtcgtgttgc tgatcaaaat
     9721 aatctccatc aggcagccct gaaagtggtc atcaagccgt tgaccgaaga ctgccaagta
     9781 gatgtgaaaa cgggtatcga tggacagcaa accaattttg gtacacagca gttggttgaa
     9841 aaagaactgc gagtatttgg tgacgatttg atcagtgcat cctaccagac aacagaaagt
     9901 aaaattgata ttgggattgc agctaaattc aataaagcag gtgtattcca agcaaaaaat
     9961 cgccggatca ccgcttcctt ttcagaaaag atcagaaaaa atacgtcctt tacgctggag
    10021 aagatcatcg tgctctctac ctctcttcag caaacagacc cgtttaagaa aagtattgcc
    10081 caagcccaga aaagcgttag ttatacagaa attttagagc agtcgatgtc ttattggaca
    10141 gatttttggc atcagcatcg gataacgatc caaggcgatt cttctttcga tcagttagcg
    10201 atggacttcg ctgcttacca tttacagatt atgactcccc gcaatgatcc aagtgttagt
    10261 gtaggggcga agggactaac tggcgaaggt tataaagggc atgttttctg ggatacggaa
    10321 atttttatcc tgcctttctt tttatatcag tttcctgaag tagcaaagaa tttgctgctg
    10381 taccgttatg atcgattaga aggcgcacgg caaaaagcaa gagattatca ctatcaaggt
    10441 gcacttttcc catgggaaag cgcacacact ggtcaggaag agacacccaa atttgctgca
    10501 ttgaatatca aaaccggaac aagacaagaa gttgcttcag ctgccgcaga acatcatatc
    10561 agtgcagacc ttgcttatgc cgttcaagag tatgtccaag caacagagga tcaatcgttt
    10621 ttagaaacaa agggaaaaac gctcattcat gaaacagcag ctttttggct gagcagagct
    10681 gtaaaagtcg atgatcattt agagatacac agagtcattg gtccagatga atacacagaa
    10741 tatatcgaca ataatgcata tacgaactat ctggcgcatt ataatgtggc cttagctgtt
    10801 aaatatcaag ctggcgatcc gctttttttg aaagaatgtc aaaatttttt agataagtta
    10861 tatttgccag tgcctaacaa agaacagctg attccgcaag atgacacgta tctcagtaag
    10921 ccagttattg atttgaccac ttacaaagaa cgtcctggca gccagtctat tttacttgat
    10981 tatgcccgta gcgaagtaaa tgaactccaa gtcagcaagc aagcagatct tgtcatgctg
    11041 ttatacttat tacctgagta tttttctcaa ccaatcgttt ctaagaattt gcattactat
    11101 gaaaacaaaa caatccacga ctcatcgttg agtaaagcga ttcacgccat agaggcttgc
    11161 cgtatccaag aaatggaatg ggcgtatcat ttattccaag aagcgtgcct gattgattta
    11221 ggaccagatc cgcattcatc agatgacgga attcatgctg cttcattagg agcactatgg
    11281 ctagcagtta tatttggttt ttcaggtatt acagtaacga atggaactct ttctatttct
    11341 ccaagacttc cttcagcatg ggaaaaaatt tcttttcctt ttatttggaa aaatcacagg
    11401 ttgctatttg ttttgacaaa aaaacaaata gtaataaaaa aagagacgaa agaaacatta
    11461 cctatttttg tcaatggaga ccaataccag ctggaaaaag aactgatctt ggaacggagg
    11521 ggaaaataat gcgtattctg attgcgccag attctttcaa agaaagtctg acagccttag
    11581 aggcagcaca agccattcaa tcaggttttc aagaagtttt tgttcaggct gaatatgatc
    11641 tgctacctat aggcgatgga ggagaaggaa cggtttcatc attagcagac agtttaaggt
    11701 tgaagcggca aacagcagtc gttactaatg catttggcaa aaacaaagaa atccagtacg
    11761 cttcagacgg tcaaactgcg atttttgaaa tggcagaaat cgtcggattg aaagatgtcc
    11821 cactaaacca acaacagcca cttaggttat ccacgcgagg agtaggagaa ctgatacgac
    11881 aattgttgca acaaggtgtt cgacacatca tcatcggcgt tggcggaagt agtaccaacg
    11941 atggcggcat cggcatggca gaaggtctag gttatgcttt ttacgatcaa gataatcaaa
    12001 aagtagaagc actgggagaa aatctaggta aaattgctac agttgataaa acgaacgtgc
    12061 ctattgagct gattaaagcc aaaatcactg tcattactga tgttactaat cttttatgcg
    12121 gaaaagaagg cgcttctttt gttttcggac cacaaaaagg cttgaaagaa aatgaattag
    12181 cagtggctga ccaagccatg catgattttt atatgagatt tgcccctcaa gtaatcgagg
    12241 catcgggcag tagtgctggt ggtggaatgg gtgcaggact gttagcattt gcttcaggga
    12301 aaatagtctc tggcatcgac tacgtattgg atttactgga gttcgataaa cgggtacagc
    12361 aagcagacct cgtaatcgta ggcgaaggac gtctagatgg acaaagtcta aaaggaaaag
    12421 cgccagttgg gattgctaga agagtaccag agggcgttcc tgtgatagct atttgcggaa
    12481 gtgttggtaa aggaagcgaa aaagcagcag atgttggcat ttcggccgtt tttccgatcc
    12541 ttcctgcagc tgtgccttta caagaagctt tacagcaagc cactgataat cttcatcgca
    12601 cagctaaaaa catcgcatct ttacttcaag tcgttcattt tcaaggaggt aatcgatgaa
    12661 aatcaaagca gcactattcg atttagatgg cgttcttgta gacacagctc gttatcatta
    12721 cgaagcgtgg ctggttttag ctaaccagct gtccattcca ttcactgaga aagaaaacga
    12781 gcaactgaaa ggaatatcca gaaccgagtc actagagcga ttgctgtcat ttggaaaaat
    12841 ggaacagaaa ttctcagaaa aagagaaatc agcctttgct gagcaaaaaa acaatctata
    12901 tcttcaagcg attcaaaaaa tggatgaaac atccgttttg cctggtgcaa tagctgttct
    12961 tgagtacttg aaaaaaacaa acatcaagat cggattggga tcagcaagca aaaatgcacg
    13021 cctcatttta gaaaaaacga atttgacaag ttattttgat gtccttattg acggcacgca
    13081 agtatcaaaa gcaaaacctg atccagaagt ttttctcaaa ggagcacagc aattgaatgt
    13141 accgccaaat gcatgcttgg tcattgaaga ttcagaagct ggttgtcaag cagctttggc
    13201 tggtaacatg catgtattgg gaataggtga aaacataaat cttccttctg ctgaatatgt
    13261 gattcctgat ttaaccgttt ttgatcaagt acgttcgttt tggcatttat cagaagccgt
    13321 atcacgtata taatagtttc cgttttttct gtcacaagca aaacgttatc ctccttttgg
    13381 aaatcaaggt atgatgagaa aaaaggggga ttttatatga caaaaaaagc agttttcgca
    13441 ggcggatgct tttggtgtat ggtcaaacca tttgaagagc agccagggat catttctgtg
    13501 acatccggtt atactggcgg gcatgtacca aatccaactt atgaacaagt aacaacaggc
    13561 acaacagggc atacagaagc agtagagatc gaatatgatc cagaagttat ttcttatgat
    13621 cagttggtgg aaatctattg gcagcaaacc gatccgactg acgcattagg acagttcgct
    13681 gatcgcggtg attcttatcg tccagtgatc tactataatg atgaagaaga acgaaatatt
    13741 gcagagcgtt caaaacaagc attgcaagac agcgggcgat tcgataagcc aatcgtgaca
    13801 aaaatcgaac caagaagcga attttatcct gcagaagatt accatcagga ttattacaag
    13861 aaaaacaagc tccattacaa tctttatcga gaaggttctg gacgtgcagg tttccttcgt
    13921 cgacattggg gcgtcaagaa ataagaagcc gaaaaagcta cttatggaac accgtatatc
    13981 agaggttgta aaaccagcat tttgagctaa tactgagggt cagctgattg aacaccattt
    14041 agtggagagg ttgtgaacct gtcgcctagc tccaagtcac aaggatttgg taagagaaca
    14101 ctatatatca gaggttgtga taagtcgttt gtcacaacct tttttcaata cctaagatga
    14161 aggacaatca gcaacgcact gctcaaaaaa ggaatgaaag gcagcttttc caactttttt
    14221 ttatgacaga taaagtaaac agaaaaaacg gatattccaa acagactagc aagaaacaag
    14281 atctgaagca actcttctag agacaagcca ccactccaag aaagcatcaa cagagtatcg
    14341 ccagctccga gataggattg accatagcga aggcacacac cgcctacgat aaacgttaac
    14401 agcacacttt gccattggaa gacgccggcc tgcaaccaaa aaatccataa aattgcccaa
    14461 gaacagaata gcgtgtgaga atggaccatg aagtaaaata aatccatcaa cgaaaatagc
    14521 aaagccgaaa gcatccatat gaacgtcgaa aaatttcttg cggaagaaaa agtaaagaac
    14581 caggcaaaaa gaccaccaca taaggcttct gccaacaaat agtagaaggg caaccgacac
    14641 ctgcaatagc ggcagcgaaa tcgttggacg atgatcgaga gaatcggaat caattcatac
    14701 caagaaagcg aacgacagca tttgacacaa tgagatctag gataaataat agagtgacct
    14761 aacggaatcc gctgggcggt taaacataaa aatgatccga tacagcaacc aatgataaaa
    14821 taaagtaaca cgccatctcc cccttttcaa aaccaagata cgcaagctta aactaaattt
    14881 ttgtacatac ataaaatttg aggtgaaaaa atgaacgttt caatcggatt agatatcgga
    14941 accacacaaa ccaaagcagt tgctttcaca gatgaagccc aagaagcagc atccgcttat
    15001 tttcgctacc ctttgatcca agaaacacaa gggatggcag aacaagatca ggaattgatc
    15061 tttcaaggtg tctgcaccgt tataaaagaa gtaattaaac agttgccttc agacacaatc
    15121 attcaatttg tctccttctc ctctgcgatg catggcctga tattgttgga tgaagagaaa
    15181 acaccgttat ctcgaatgat cacttgggca gataaccgag cagcggacga agcagaaaaa
    15241 ctgaaatata ccgtgcttgg caaagaattt tatcggcaga ctgggacgcc gattcatcca
    15301 atgacaccat tgaacaaaat caagtggctg caaaacagcc aacctgaatt attcaaaaaa
    15361 actgcttatt ttgtcggtat caaagactat atcttttata gattgtttgg cgaactgatc
    15421 agcgactacg gaacagcttc aggaactgga tattttgata tccatgaatt ccaatgggca
    15481 gaagaagtgc ttgactactt gaacattcga atagagcaat tgccgcaagt ctttccttcc
    15541 acctatcaag taacaggtct accatcaaaa acagcagcag aactaggttt gccaaatgat
    15601 atatcatttg ttttgggagg ggcagacggg ccgctttcta atctagggtt aggtgctttt
    15661 ggtgatacga tcgccgcttt gaccgtagga acgagcggag cacttcgttt tattactact
    15721 cagcctcagt tccatccaga aatggagacg ttttgttatg ttctggatca gaaacattgg
    15781 gtagtgggag gagcgactag caatggtgct ggcatctttg attgggcagg gcatacattt
    15841 ttacaggaag tcgttcaatc tgctaaagaa aaaggagaaa atccatatga tgcactgctt
    15901 tcggctatcg cttttgtgcc agcgggtgcc aatggtctga tttttcatcc ctatttactt
    15961 ggtgagcgag caccgctttg ggacgcagat gcgtttggca gttttatcgg tttacagcgg
    16021 caacatacag aaaaagaaat gatgaaggcg attctagaag ggatctgtct caacctttat
    16081 cggattttaa aaggagtttg ccaggaaaat cagctcacag agatccgtgc tacaggcggg
    16141 tttgctcaat ctccgatttt tcgtcagatg atggctgatg tactgggtta cccgcttgtt
    16201 ttccctactg tcacagaagc atccgcatta ggagctgtga ttttaggctg gagaagcatc
    16261 ggaaagatcc aaagtatcag tgaagcacag gaattgatag aattgaaaga gcaagttgga
    16321 gtacaagaaa ctgatcataa aaaatatcag aaaatctatc cattatttgt ttcgacacaa
    16381 aagcatttag cacagactta tcagcaattt gctcaactgc gagaagaatt agctgattct
    16441 gaaaaatgat tggctttacg gataaaaaag cgaactgaaa atcgttctca ggtaaatgat
    16501 tttgttgtac ttttcattga aagtctacta ttattttaag tgtaagaaaa tgttaggagg
    16561 caagaaagat gaaattccaa caaaccaaag aagtattaaa tcaattagtt gcagatctaa
    16621 gccaaatgtc tgttgttgtt caccaaacac attggtacat gagaggacca ggtttcctta
    16681 cattgcaccc aatgatggat gagttcatgg atgatttgaa cgctcagtta gatgaagtat
    16741 ccgaacgctt gatcacatta gacggttcac cttattctac attgcgtgaa tttgcagata
    16801 acacaaaaat ccctgatgaa attggccgct gggatcgtac tattccagaa cgtttggaaa
    16861 cacttgttgc aggctatcgt tatctagcag atctttacca aaaaggaatc gaagtttctg
    16921 gagaagaagg cgacgatcca acacaagata tctttatcga attcaaaaca gccacagaaa
    16981 aacgtatctg gatgatccaa gctcatttgg gcaaagcgcc agagatcgac gcgtaaaaac
    17041 tgttgttgaa cttgaccgaa aaagaggttg tgaaatcagc gttttgacct gagtattaga
    17101 gaaacaaacg tcaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    17161 agctgattga acaccgttta ccaagaggtt gtaagcctgc cgttcagctc cgagtcacaa
    17221 gaaacaattt taaaaaacag cttcttatgt tttatggata ttgtgacttg tgaccgagga
    17281 gttggcagga gaacaccgtt tattcaaaat aaggggttgt gacaagactt ttgtcacagc
    17341 ctctttttgt ttttcatttt gacaggaaac gaccctttta tataaaagaa agaaaagaag
    17401 taaagtttca tataaatttt tcaatcaagc agaaaaacgt tgacattgac tagattagcg
    17461 ggtaatatgt taaatggtat tgtttgcccc acaaagagcc ccggaaactc gaagtgtatg
    17521 tcaaacatca gaacttaaat tggaggaaaa taacgtgcgt acaacatata tggccaaacc
    17581 aggcgaagta gaacgtaaat ggtatgtagt agacgcaact gatgttccat tgggacgtct
    17641 ttcaacagtt gttgcatctg tactacgtgg taaaaataaa ccaactttca cacctcattt
    17701 ggatactggc gatttcgtca tcgtcatcaa tgcagacaaa gtgaaattaa caggtaaaaa
    17761 agcgactgac aaaatttatt accgtcacag caactaccca ggaggattga aatcaatcac
    17821 tgctggcgaa ttgcgtgcta aaaattctcg tcgtttgatc gaaacttctg taaaaggtat
    17881 gcttcctaaa aacactttag gccgcaaaca atttacaaaa ttgaacgtat acggtggagc
    17941 agaacatcca catgcagcac aacaaccaga agttttagac atcacaaact taatctaata
    18001 ggagggaatt tcattggcac aagttcaata tatcggcaca ggccgtcgta aaaacgcagt
    18061 tgctcgcgta cgtctagtac caggaactgg caaaattact gtaaataaaa aagatgttga
    18121 agaatacatt ccacatgctg acttgcgtga agtaatcaat caacctttcg ctgttactga
    18181 aacaaaaggc gcatatgacg tatttgttaa cgtaaacggc ggaggatacg ctggacaatc
    18241 aggagctatc cgtcatggta tcgctcgtgc attgctagaa gttgatcctg acttccgttt
    18301 agctcttaaa cgtgctggct tacttactcg tgacgcacgt atggtcgaac gtaaaaaacc
    18361 aggtcttaag aaagcacgta aagcttcaca attctcaaaa cgttaatatc ctttatatat
    18421 caacgtttct ggcacttttc aattatttgg aaagtgcttc ttttgttttt aagaacacat
    18481 ttaagaacac atatcaatca cacttattca atcctctctg aaaagcttca aaaacagcct
    18541 tatcaactat atctggtgtg ttcacatata tctttgttgt agaaatatct gagtgtgtta
    18601 aagcatttga aatttgattc atatctgctc caccttgtct agctatagtt gcataagtat
    18661 gacggagttt gtgtggagat aaatgaacta agtcaggatg ccttctttta attgaattga
    18721 ttcgatagtt taggtaatcg gcatgaacag gacaatttac ttctccagat ggtttagtat
    18781 aagtaaagag atactgatct aacgtttgct taatttttaa tttcaacaat tgatcagctt
    18841 gtaccgattt ccattcactt agcaatgtca ttacaacttc tggtacttga atgacggtat
    18901 cttttcgacc ttttgtaaac ttttttcttt gatatttatc taaagtatgt ttaagacgga
    18961 ccgtttgttt ttcaaaatca atatatttcc attgcaaagc gtaagtctca ctttttctat
    19021 caccaagata cagggtaatc ataaaaagtg tgtaatcatg aaaagtaagt aaatgattat
    19081 taaaatcatc atttactgct tctatccaat tagtaagttc ttttgatgat agtgcttgct
    19141 taccttcttg tattcttttt tcttctaaag catttttttt aggtgcagtg atactttgaa
    19201 taattttagt agttcgatta aattcgatat aatttagtat ttctgcaata tcgaacatgg
    19261 aacgaacata tccttttatg atttttatat ttgcatattc tttcgatttt ttggttaatt
    19321 catctgatat tatctcagta ttgatattta gataattcat tgcgtattca ccaaacatag
    19381 gtaaaatatg caaccgaaat atatcttttg tattactaat ggttatatca ctaggaggcc
    19441 tgtttgatct aattgtttga cctaattcat atctaggaag ccatttagat tcgtaaaatt
    19501 ttttaaacgt tatttttcct tttaattcta atgagtttgc attctcctct ctcaaaactt
    19561 tttctatttt cttcttcata tcattttcgg ctttaatcgc caattgccta tttggaaatg
    19621 ttttttgata cttttctgat gcaattcctg ttatttcttt aaaccttttt ggatatctca
    19681 gattagtttt atatttccca tttgaaa
//
LOCUS       DADXTG010000045        19063 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.046, whole genome shotgun sequence.
ACCESSION   DADXTG010000045 DADXTG010000000
VERSION     DADXTG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.046"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(101..1054)
                     /locus_tag="IWI57_001351"
     CDS             complement(101..1054)
                     /locus_tag="IWI57_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2449503.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1077..1832)
                     /locus_tag="IWI57_001352"
     CDS             complement(1077..1832)
                     /locus_tag="IWI57_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2449504.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(1829..2788)
                     /locus_tag="IWI57_001353"
     CDS             complement(1829..2788)
                     /locus_tag="IWI57_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBC2449505.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(2781..3731)
                     /locus_tag="IWI57_001354"
     CDS             complement(2781..3731)
                     /locus_tag="IWI57_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBC2449506.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            3881..4333
                     /locus_tag="IWI57_001355"
     CDS             3881..4333
                     /locus_tag="IWI57_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBC2449507.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4422..6689)
                     /gene="gshAB"
                     /locus_tag="IWI57_001356"
     CDS             complement(4422..6689)
                     /gene="gshAB"
                     /locus_tag="IWI57_001356"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBC2449508.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(6876..7874)
                     /gene="ftsY"
                     /locus_tag="IWI57_001357"
     CDS             complement(6876..7874)
                     /gene="ftsY"
                     /locus_tag="IWI57_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBC2449509.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(7885..8694)
                     /locus_tag="IWI57_001358"
     CDS             complement(7885..8694)
                     /locus_tag="IWI57_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2449510.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8703..12284)
                     /gene="smc"
                     /locus_tag="IWI57_001359"
     CDS             complement(8703..12284)
                     /gene="smc"
                     /locus_tag="IWI57_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBC2449511.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12304..12990)
                     /locus_tag="IWI57_001360"
     CDS             complement(12304..12990)
                     /locus_tag="IWI57_001360"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBC2449512.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13338..15119)
                     /locus_tag="IWI57_001361"
     CDS             complement(13338..15119)
                     /locus_tag="IWI57_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2449513.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15145..16059)
                     /locus_tag="IWI57_001362"
     CDS             complement(15145..16059)
                     /locus_tag="IWI57_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449514.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16086..17048)
                     /locus_tag="IWI57_001363"
     CDS             complement(16086..17048)
                     /locus_tag="IWI57_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449515.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17048..17992)
                     /locus_tag="IWI57_001364"
     CDS             complement(17048..17992)
                     /locus_tag="IWI57_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449516.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(17993..18997)
                     /locus_tag="IWI57_001365"
     CDS             complement(17993..18997)
                     /locus_tag="IWI57_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449517.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5221 a         4144 c         3384 g         6314 t
ORIGIN      
        1 tggtttttga taacttcttt gaggataggc tcttgctatt taagaatgta agaaacatta
       61 ttgttacata gtatgagagt ttaatcctct gttatttatg ctatttaaat gcttgattca
      121 catcttcgat catcagtttc attgattcta aaccgccccc gcttaaatac cagacatctg
      181 gttcaaggga gatgatctgc tgatttttac cagcattcgt ttgtgctacc agttcattgg
      241 cagaaatatc atctttgctg tcgtctccac caatagcttt tgttctgtct actacaaata
      301 agatatccgg atttttttcc agcacatatt cataagaaac actttgaccg tgagtggatg
      361 cttcgatttg atcatctgct tgttcaaagc caaataaatc atggatgaaa ccgaatctag
      421 aaccagatcc gtaggctgat aattgccctt cattcacaag tgtgactagt gcttttttgt
      481 cagtagcttc tgctttcttt ttcgtctgat cgattgcact agataattcc tctaattgag
      541 ttttagcttc ttcttgctta ccaaagatct cccctaaagc agtgacattt tgctggaaag
      601 attcccatgg atttttcgca tctaaagaaa ggaaaatggt cggagcaatt tgacttagat
      661 ctttttgaaa atctctttgt cggccagaaa tgatgattag atctggctga agctgattaa
      721 ttttttctaa gtctggttct tttattccac ctgctgattc tactttttta taggatgaaa
      781 gatactcagg aaggctgctt gtagctgctc caactacctt atctccaaca ccaagagcat
      841 ccatcgtatc taaagaacca ttgtcgaata cgacgacttt ttctggatta tttggcacct
      901 cgatttggtc tccattacta tctgttatcg tcaatgtagt ttcagtttgt gtgctgcttt
      961 tttttgtgtc tgcctgactg gtgttgttgc tatttccaca tgctgctaaa ccaaatgatg
     1021 ctagaatcaa gatacctact actaattttt tcatctcttc tctcctaatg ttagttttag
     1081 aaataaagac agaatctgcg gccttcaatc tcacaaatcc gtatgtccat ttcgtataat
     1141 tcatccaata catctttttt tatcatttct tgggtcgttc cttgcgtaaa taactgtcca
     1201 tctttcattg caacaatttc gtctgcataa cttgcagcaa agttgatatc atgaagaaca
     1261 ataacaattg ttttcccgat ttcttgtact aaatttttta aaatctgcat gatttgaacc
     1321 gcaaaattca tatctagatt gttcaaaggt tcatccagta aaatataatc cgtgtcttgc
     1381 gctagcacca ttgcaatata cgcacgttgg agttgaccgc ctgaaagagt gtcaatcgat
     1441 tgatccgcta tttcttctaa cccaaggtgt tccagtgatt cctgaacgat ttcctcgtct
     1501 tttgtcgtca attttccttt cgtataagga aatcgtccaa attctactaa ttctttcacg
     1561 gtgatttgga catttatttc attcgattgt cgtaggatcg ataatttttt ggctaattca
     1621 ttggatttcc aagcctttac ttcgttatgg tctaaataga tttcacctgt atcttttggt
     1681 atcaaacgac tgatcatcga caacaccgtt gactttccag caccatttgg accgataaaa
     1741 gcagtcaact tcttttcagg aatcgttaac tgtacatctt taaccactga ttttccatgg
     1801 tacagtttat tgaccttttg taaatcaatc ataggatact cttcctttcg cgaatcaatt
     1861 ttgtgacaaa ataaattcct ccgacaaatt caacgacgac actcactgtt gtattccagc
     1921 cgaaaatctg ctctactgcg agctgtcctc ctgtcagaaa cagcatagct accagaaagc
     1981 ttccgataaa tagctgacca tgacgataag tagaaaagac acgataactg atatttgcga
     2041 caataaaccc taaaaaagta actggaccca caagagccgt tgatgtccct gtcaaaagac
     2101 tgacacaaca taaaatcaaa aattcaaaac gatctgtgtc tatcccgagg tttactgctt
     2161 gttcatttcc gagatggaaa acgtctagca catgtcgata tcgaaataac aggtaccagc
     2221 agccgataat gatcaaagaa gcaggcaaaa gatactgact ctggatattg ccaaagcttg
     2281 caaataatct gccttgaagc aaatcatatt cattcggatc catgatgact tgtaaaaagg
     2341 tactgacact accaaaaagc gtacctaaaa tcatccctga catcagtaat aaaaaaagat
     2401 tcgctctcat ttttttcaaa agaaagaaaa ataacaatag actcagcccg accatcagcc
     2461 caacgttcaa taagaaaaaa actgtacctg tctgctgaag catcgtcact cctccgataa
     2521 agaagaaaag gaccgtttgg acaaggacat ataacgactc aagtcccaat actgaaggag
     2581 tcaaaaactg gttatgcgta attgtctgaa agctgattgt ggcactgctg acagctagcg
     2641 agacaaggat aaatgcaaaa attttcttac tcctcaatgc acatgcaaaa tgccagtttc
     2701 catatgtatg gatcgtcaag tacattcctg taacaaataa aacaagaaca agcataccaa
     2761 taaaaataga tggctttttt ttaagcatat cgtcgtcccc ttatcagaag ataaaggaag
     2821 atagccccac cgatgattcc aacgatcaca ctgactggta tttcataagg aaaaatcaac
     2881 acgcgagcga caagatcgca aaatactaag aacaaactac ctgctaaagc agttacgcca
     2941 attgttttgt gcacttgatc tccatatatt ctagagacaa gattcggaat gatcactccg
     3001 ataaaaggaa tgcttcctac catgatcaac atgactgcac tagccaatga tactaagcca
     3061 atgcctaaaa actgcatctt ctggtaatct attcccagat ttactgcaaa cgattcgccc
     3121 agaccaatga tcgtaaatcg gtaagcaaat agccaaacca agagcaacaa tggaacaaca
     3181 gcatacaata actcataatt tcctcgtatc acagtcgcaa aattcccttg aagccatgaa
     3241 gtcatatttt gtaccaactg gaattgataa gcaaaaaaag tagcaactga accaatgata
     3301 ttccctaaca tgactccaat caacggaagc atcaattgat ttttctggag gaacaatcgc
     3361 gtaagggata aaaaaagcaa tgttcctgca aaagcaaagc aaaatgctgt caaggatcgg
     3421 acaatcaagg atgcaccagg taggaaaatc atagccacta aaatacctaa acgcgcacta
     3481 tccatcgtgc ccgcagtcgt tggtgaaaca aatttatttt gggtcaaatg ttgcatgatc
     3541 aacccacaaa tactactggt cgctcccaca aggatcaaac aaatcgttcg tggcaaacga
     3601 gtcactagca agatttgtaa gctttgccca tctaactgga gcagttctga caattgtaca
     3661 ttcgatactc cgatgaacaa tgaaatacta cttaaaataa ctaataatgc tactagtcgt
     3721 ccgtagcgca tccaatccac ctttctaatt gagaatgatt ttcattctta ttttaagtga
     3781 atcgttgaac aaaagcaacc gctttttcat atgttttagg tattattggg aatatgctat
     3841 aatcgtaaga aagacaaaga gaagaaggag ggtatttttt atggcacaag atgatgtaaa
     3901 aagccatttg gataagggac tttatggtac acctttagtg aaccctgaag aacagcataa
     3961 atatttaggg acattcaggg aacgctgcta tcttagtatg actgttgctg aaatgatcca
     4021 accaaaaaac aaagaaaact ttttaaaaga actcaaaaaa catccagatg cttccatact
     4081 cttgaatggg gctctacccc tttctgtcca aaaccaatat atccagctag caacgaaaaa
     4141 caatagccgt ttcactgtag tcaacgatta cgtcactgca aatcctgagg aattcggatt
     4201 gctcttaaca gcaaaagaag cagtaaatga acctgtgatt gatatcgaaa aaaaataccc
     4261 agaagaaaac tcttcgccag ctgaaaaaga aaaaccaaaa aagaaaaaaa gcttctggga
     4321 tcgtttattt taaaacgaaa aaacacataa atttttacat gcaaaaaaag cttttgatca
     4381 gattgacatc aaataggaca atctgtcaaa agctttttct tttaaaatac ttcaggatat
     4441 aacaatttca atacattcat cgtcaatctt ctgccttttc ctgcaaaagg ataaacatgc
     4501 atatgcatgg ctggattgaa atttgcttcg atgatcccat atgctttttt atcagttgta
     4561 ggatcgattt ctttatctgg tataatcaaa tcgataccac taatcttagc tcctagtgct
     4621 tctaccgcag aaacggcgat ttttttgtaa gcttcagaaa attcatcagt catatcgatc
     4681 gaatcaccac ctgtactgat atttgagttc tcccttagat aaacaatttg attggcttgg
     4741 ggaactgatt cgatcgtcaa cccttgttct ttcaacatca attgttctaa ttcacctaat
     4801 tggatcaatt ctaaaggtgc acgatgattc gttccacgca aagggtcact atttttttct
     4861 tcaacaagtt ctttcactgt tcggatactg tcaccaataa cattagcagg gactcgcaac
     4921 atgatggctt ggacttctcc gtcgatcaca aagaaacgat attccgttcc cggcataaat
     4981 tcttctacta aaaccgaact gtcttcacga aaagcaatgg ctaaacctgc ttgataatct
     5041 tccagactag ctccttcctt gaaaatcgtg atacctaacc cgtaattggt tgatttaggt
     5101 ttgatcacaa atgcctgctc ggcaaatctc ggataagctt ttaccgcttc ttccatggat
     5161 gaaaattctg cgcctcctgg tacccggaac ccagcttctt ttaagacttt tttcgttact
     5221 gttttgtttt ccatgataag tgggacgata tagctgtcct tacttgtcat gtttgcattt
     5281 ttcacatatt ctacatggtc ttggaattgc agacgcaaaa attgatccga ctcatctaat
     5341 actttgactt ggacaccttt ttggatggca tcgaacataa atatctgagt agaaagttcc
     5401 atttcgcgaa aaccggccaa ctgataggga cgttcatggc tttctttata atattgatgt
     5461 cccaatgcca cagcaacttc ttgctgactc gatagctgtg cttcttgata gagtttcgct
     5521 gctaacgttt gttcaggatg atccatttga gtcaatgctt cctctactaa ataaagagaa
     5581 tccaaccaat ccaattcaac cagcatttgc ttcattcctt caagaatttc tctaccttct
     5641 tgatgaaatt ctgtttgatc tgatggttgt tctagtgcta ctttgttatt tcgagtcgta
     5701 ccttctgcta cccattggtc acagtcttct ttttcttctg tccataacat gtacatcagg
     5761 aataattgta agaaccgaac ttgctctgga ttgataccca atcttgcata aggattcaaa
     5821 tcgatattcc gcaattcaat ataacgaaca cctgttgttg ctaaatctgc gactttcttt
     5881 ccgcctcgga aacgaagcgg tgtataaaat tctttctcct ccgaaagttt cccttcttcg
     5941 atcattcgct cgatatctgc taaatactgt ttcatagaag cataggaaac tttcacattt
     6001 ggatgattcg tatagcccag cgcactgttg cgaatactgc gaacaggttc ttgcggatgt
     6061 gattcatcta aaaagtagtt cttctcactc attggtgaag caccgaataa ataagtgatc
     6121 atccagcgat agcgcataaa gtttctcgct gttttcaaat aaagttctgt tttgaattcg
     6181 gaaaaatctt gaaattcttc ctgatggctg aacaatgttc ttagcaactc atcgccaaat
     6241 tcaaaattga aatgaatacc gcttaccatc tgttttcgct tcccgtattc ttttgctaaa
     6301 taacgtcgat acaagacatc ttcgaaattt tttaattttg caataatgat ctcttcgtct
     6361 ttttccggta aggcaggtgg catacttaat ggccagatca tctcttcttt cggtatagaa
     6421 cgagcagtca catcataaac agcagcgaga tactgaaata attcgggaat agaatcggta
     6481 acaggggtga tcatctctgt ttgtgtttcg ctaaaatctg tttggatata ggggtgatag
     6541 gatcgatcgc caaagattgc tggatgatcg gtttttgcta gatttcctgc aagatcaacc
     6601 cgctgtcctt cccgttctat accaaatcga gcttggtcga taaaagggcg cgcattgaca
     6661 tgcaataata attgcttaaa attcatcatt aagtgacctc caattcatat gaatctaatt
     6721 ataatcaaaa agtctaagaa aaagaacgaa attgtttgag aaccgcttac aaataagtat
     6781 aaaaatgaag gcatcaaaaa cacagctatg ttaaaaacaa tcattcaaac aaggagctgc
     6841 aacttttgtc acagctcctg ctttttagac atgggttatt cttcttttag taatcctttg
     6901 aacaatccaa cgacaaaatc atttggatca aacggctcca agtcatcgat tccttcacca
     6961 agaccgacca atttcactgg taaatgcaat tcattacgga tagcaagtac gataccgccc
     7021 tttgctgtac catctaattt tgtcaaaaca agaccggtca catctgttgt ttctttgaat
     7081 tgttttgctt gaaccatcgc gttttgacca gtggttgcat ctaatacaag aagtgtttct
     7141 tggggtgctt ctggtaattc ccgctggatt actcgtttta ttttttctaa ttcgttcatc
     7201 aagttcactt tattttgcag tcggcctgca gtatcaacta gtaaaacatc tgcctgctct
     7261 gtctttgctc tttccaaagc atcgaatacg acagctgctg gatcgcctcc tgcattgcca
     7321 cgaacaactt caacaccagc gcgttcgccc cagacaacta actggtcgat tgctccagcg
     7381 cggaaggtat ctgctgctgc catcaatact tttttacctt cgtttttgaa ctgatgggca
     7441 agtttaccaa tacttgttgt tttccctaca ccattaacac cgacaaacaa aataactgtt
     7501 agtccgtctt cttggatatt tagttcattt acttcattga ttccttccgc ttcatagaga
     7561 tccacaagtt tctcgatgat cgcattttgg acttctgccg attttttcgc atttctgagt
     7621 ttcacttctt gacgtaatgc ttcggtgatt cgtatcgctg tttcaaaacc tacatctgca
     7681 ccgataagtg tttcttctaa ttcttcaaaa aagtcttcat cgactgttcg aaaattggca
     7741 aacaactcat tcatccgttc accaaatgtt ttacgcgtct tttctaaacc cttgtcgtac
     7801 ttctcctgta cttcaacttc ttctgttttc tcttcgccag taaaagcacg cttgatttta
     7861 tcaaaaaatc ccatttgact ccccctactc taatacaaat ttttggatca ctttggctac
     7921 gccattttct tcattgctgg ctgtcacgaa atctgctgct tctttgacga aaggcacagc
     7981 attctccatc gctacgccta gtccagcata ttggatcatc ggtaaatcat tttcctcatc
     8041 accgatcgcc atgatttctt cttgtttcaa accaagatct tttgccaata aagaaatacc
     8101 ataagctttt gtaacacctt ttggcataaa ttccaaaagc atacttcttg ttttgatgat
     8161 ctcatacatt tcatggaatt ctgctggtat ttctttgatt ttaggatcta attcttcttg
     8221 gtcataagcg attactgcct tattaagcac aaattcatct gtaatatcgc ttatatccgc
     8281 tggttggaat tgcagcagct tattcaacac attgtacaag gattgcttcc ttggggcact
     8341 aggcagctgt aaaaccacat tgtcagacaa aacatctagt ggcagatcca attgctgagc
     8401 cagtttgtat aagcgttgga tatctgatac ttccatcact ttcttttcca tgatttcacc
     8461 agtatcattt ttttgtacca aaccgccgtt gaaagtgatg ctgtagtcac cttcatctac
     8521 cagtcctaat tcttgcagat aatgcgccat cgcagctaag gggcgtcctg tacatagaac
     8581 gatcttgacc ccttgctgtt ttgcttgtgc taatgcttgt ttattctcat ctgaaatcag
     8641 cttttgttca tttaataatg taccgtccaa atcgattgct actaatttga tcattttcgt
     8701 tcctacactt tcgtttcgtt gattttcgcc tcttcattga tatcttccat ccgtacagaa
     8761 acgatttttg atacaccgga ttcctgcatc gtcactccgt acagtacatc tgctgcgacc
     8821 atcgttcctt tgcggtgtgt gactacgata aattgtgtgt catcttggaa atcgctcaag
     8881 taacgaccaa aacgtttcac gttggcttca tctaacgctg cttctacctc atctaagata
     8941 caaaacggaa caggacagac acggataatg gaaaagagca acgcgatggc tgtaagtgct
     9001 cgttctcctc cagaaagcaa acttagactt tgcagttttt tgccaggagg ttgtacttca
     9061 atctcaatac ctgttttcag caaatcagtt ggttccgtca gtactaattc agctttaccg
     9121 ccgccaaaca tatttggaaa gacaattttg aattcttgac ggatagcttc aaatacttct
     9181 ttgaatcttg tccgcacttc atcatccatc tcatccatcg tttcaaacaa ttggtttttc
     9241 gctgctaaca gatcatctcg ctgtgttgct aagaatgtat gtctttcact gacttgttcg
     9301 tattgttcga ttgaatttaa gtttactggt cccagttttt caatctgttc ttttaaggat
     9361 gaaacctttg ttctactgtc ttcgatatcg gttgtttctt gatagtcttg ggaagctttc
     9421 tcaaatgtca gctgatattc tgactgcaga taggtcaaat gattatctaa aatcatttca
     9481 gctcggtctt tttggacttc taatttggat tgttccgaca gtcgtgcttt ttgctcacga
     9541 ttacgctctg ccaagactgc ttcaagctta tcgatctcct tttgccttct atctcgctgc
     9601 tctttggctt ttgccaactc ggcttttaat gtttctcgct gagcagataa ttcgttaatt
     9661 tgtttttcca aactttcttc tgtgacttca tgatcagaaa agtctgctgt caatgtcgcc
     9721 aactgctttt ctatcgcttc ttgacgttcg gttgcctctg ctttttggac tcgggcaccg
     9781 cggagctgga tctgcagatg attaaactgc tctttcaaga cagcaagatc tgcttgttct
     9841 tgtgcttttt gactttgtac ttgtgctctt ctggcttcca tctggtcgct ttcttgactt
     9901 aacgatttga tttcttcgtc gattttttgt cgttgcgatt caagatctgt ttgtttgtct
     9961 ttcaattcgt tcgtttgctt ttcgtaatct tcgataaact gctgcagttc acgtgtctca
    10021 aaagttgaga tttgcttttc tttttcaaaa cgttcaagtt catttatgat gttttgtagc
    10081 tgatttgtcg cttcttgttc ttcaaaacgt aattgttcgc ctcgtgtacg caaaacttct
    10141 tgttcttcgg ctaaacgagc tgtttcttgt tgcagctcct gaactttttt ttcttgtgct
    10201 tggagttgtt tatctgcttc ttcaaattca ctagtcaact gtttcaattc ttgattttgt
    10261 acaaacaaac tgccttggtt tcctcgttta ttcgctccac ctgtcatgga tcctccggca
    10321 ttcatcacat caccttctaa ggaaaccact cgatattgat agcggatcgt ttgcgcgatt
    10381 gcattggcac tagttaagtc tttggctaaa aggatcgttc ctagcaggtt ttgaaccaca
    10441 gtttggattt gatcgggata actgacttgt tcactggcaa tccctataaa cccttctaca
    10501 gctgctgctt gtgtcaatat atgagccggt aattgtcttg gtttgattgt cgtcagtgga
    10561 aggaatgttg ctcttcctcc tctttgctgt ttcaaatacg tgatggcttg ccgagcatct
    10621 ttttcatttt caacgatgac atgctgggct gccccaccta aagctgcttc gatagctaac
    10681 gtgaaatctg caggaacatc gatcagttca gccactgctc cgacaatacc cgaaagctgc
    10741 tgtttatgct gcaagaccaa tcgaacccct tggtaaaaac caaaatagtt ttcttggatt
    10801 tcttgaaggc ttttcttacg agcacgtagc tgctggactt gattcattaa ttgatacatt
    10861 ttaggctgtt cttttgctaa tgcttcttgg aactttttcc cgttttgttg gatcatttct
    10921 aacttctctt ttgtttcagt taaagatgat tttagctgcg cctgcttttg tgtcaattct
    10981 tctttttgta agatcaatcg gtctactgat gcttctactt ccgattgctt ggctaacgtc
    11041 tgcttgcttt ttgctgtttc ttgtatatat tgacgttcta gatacttcag ttcgtttcct
    11101 acagctgctt gttcttgcat caaatcaaca tattgatctc ttaattctgc caataattct
    11161 ttcgttgatt tttggtattt ttctaattct tgctgtgttt ttatgagatt tgcttcagct
    11221 ttttggatct ctgtttcttt ttctgctgct gctttcatca agctttcttg aagcttttca
    11281 aaatgtttaa cctttttttg tgcttctgct aatgaagttt gatattcttg cgagcttttt
    11341 tgtgtatgct ttgtccgctc ctgcaaaaca tctttttgtc cttctgtctg cttcagtttt
    11401 tccgaaagat ctaataatac ttgctgattt ttttcgatca gacgatcggc ttgcgcattt
    11461 tctttacgct gcttcgccaa aatggattct tgttcttgga tcgactcaga tagtttgcct
    11521 aattctagat tgaatttcgc aagctgtgct tgtttgttgt cccaatcttt ttttgctgtc
    11581 ttgatttcag caaccatcaa agagacatcg gtttgagtca gtgtttcttt tagccgtaaa
    11641 aactcctttg ctgcctcact ctgggcggcc aaaggagtta actgttcttc caattcgtgg
    11701 atgatgtctt ggacgcggct gaggttgtct tctgtttcaa aaagcttctg ttctgctttc
    11761 tttttccgct gcttgtattt caagacacca gctgcttctt caaagatccc acgacggtct
    11821 tccggtttac tactgaaaat cgcttctact tttccttgag aaatgattga gaacgattct
    11881 tttcctaatc cagaatctaa gaacaattct tggatatctt ttaatctgca gctttgttta
    11941 ttgatgaaaa actcgctttc cccagtccgt cgataacggc gggttacact gatctcattg
    12001 tactccaatg gcagataatg atcactattg tctaaaatca ctgtgacttc tgcaatattc
    12061 agctgcttac gggtatcgga accggcaaag atgatatccg gcatttttcc gcctcgcaga
    12121 cttttagcag attgttctcc taatacccag cggatagcct ctgtaatatt gctttttccg
    12181 ctgccatttg gtccaaccac tgctgtgacg ctattttcaa aatcaatgac tgtcttgtct
    12241 gcaaatgatt taaaaccggc tatttcaatt cgtttcaaat acaaagcttt ccctcactta
    12301 cttttacagt gctgccaatg cattttccgc cgcagcttgt tctgctaatt ttttcgattt
    12361 cccttgtcca gtgccaatca attgatcatc tacataaact tcgatccaaa atactcgttc
    12421 atgagctgga ccttcttcat tgatcaatcg gtattcgatt gatacatcac ctgatttttg
    12481 caagacttct tgtagttttg ttttatgatc catctcatgt gaaaaagcac ctgctttgac
    12541 tttaggaaaa atcacttttt cgatgaatgc atgggtcgtg tcgaatccct ggtcaagata
    12601 taacgcgcct aaaaaagctt cgaataggtc gcaaagcaat gctggtcgcg tacgtcctcc
    12661 tgaattctct tctcccttgc ccaacaaaac atattgatca aagtgacatt ctttggcaaa
    12721 tttcgacaag ctgtcttcgc gaacgatcgc agctcgtgtt tttgtcaatt ttccttctgg
    12781 caaatgagga tataaacgga ataaatactg agaaaccatc agttctaaaa cggcatctcc
    12841 taaaaattca agtcgctcat tatccgataa ttgcagattg cgatgctcat tcacatagga
    12901 tgaatgggta aaagcttgtt caagcaaatt tagatcgtga aaaacgatgt tatagttttc
    12961 ttttagttct tttgtcagct ggttgtccat tttccacatt ccttttatgg atattttttc
    13021 gatacttcct tattatactg gttttttgtc aaatttgaaa tggttcggtc aaaaggtaag
    13081 taaagcttta ataatcctag tgattgacga gaagttgtga aaaattcgtt tagtttcgag
    13141 tatgaagaaa aaattttgtg aaaatggctt tccatatttt tgcataaatt tgacttaata
    13201 ccgagaaact ggactttgaa caccgtttat tggagaagtc atgaaatcag cgccttgacc
    13261 tgaatattag agaaataaac agaaaaatat caaaaaaccg aaaaaggaca tcgccctttt
    13321 tcggtcattt ttagtgatta tgaacgtgaa tctgctgtaa cagaaacatt tgcccatggg
    13381 tttggtacat cataagacca atcccagtct ttgacacggt cgttgactgg caatacttca
    13441 ttacggaaca acgttggaat cgcaaatgct tcttcaaatg catattgttg ccatttttga
    13501 tatgcttctt tttggtactc ttcatcaaat gctttgcttg aactgatatc gttgatcaat
    13561 tttgtatttt cttctgattc ataacgagtg tagttgaacg cagcatttgg tccccatagt
    13621 ccacttggat ttggatcagt tcctgtattc caagcagctt ggtaaacatc aatttcagga
    13681 tcatcgtttt ctaatttatc atagaaagaa ttgaattcga tcagacgacc tgttgtgtat
    13741 tggacatcca aaccaatttc tttccattgt tggatatagt aatctgccaa tggttgcgct
    13801 gtttccccac cggacatcga agcaaagttg attgttagtt tatttccatc tttgtcttct
    13861 cttaaaccat cgtcatccac atctttgtag ccagcatcat ctaatagttt tttcgctttg
    13921 tctagatctt gtgtgaatcc tttcatctca tcatcatggt atgactggaa tactggagga
    13981 atcaatgaag tcgcattgct tctcaaacct gcatagaaac gttcacctac tgcattatta
    14041 tctaatgcat agcccattgc ttgacgtaat gatttatcag ccatttttga atctggattg
    14101 tattccacac tctttgtatc tgcattccat ttacctaact tgaatcctag ataagtataa
    14161 gaagtttgtt cacggcctaa gtttgtatag ccagcaattt ctttataggt atcgtaagta
    14221 tctgttggca tttggaaaat catatcatat aatttagagt tcaatgcttc agttgcactt
    14281 gccatcggta cagatttcat cgtgatcttt ttcaatttag gcgtaccatt ccagtagtgc
    14341 tcgtttggca agtattctac tgattctcct gcaacgatat tactcatgta ataagggcca
    14401 aaagtcactg gatttttacg tacttgatcg cttgattcca tatctttgat tgctacacct
    14461 tctaacgtat gttttggtgc tgcatatgac cagataccac cacccgattg aagcatgctt
    14521 gggtttactt ctttatattc gatttcaact gttttgtcgt ctattttttt gattccggag
    14581 attgtgtcgg cacttccatc atgatattct tccataccta cgatgttacg gaaagtgtcg
    14641 tcataacgga taccagtgta atctttattc ccaatgattt cgtaagggaa aatcacatcg
    14701 tccgccgtta catcttcacc atctgaccat ttgacgttgt cttttacttt gattgtcgct
    14761 tttttcgtat cttcatctag atcaagcgtt gcagctccgt cgtctgtgat tctgaaatcg
    14821 tcatcgtttg caaatagcgg ttcatgagaa ggagccataa aagctgcatc atatgaatct
    14881 tggtaaaact cccactggaa caatccttta aactgactgt ccgttgcgac cgcagcttct
    14941 aattcgccgt cttcgattgc atcatcatta ttttttgttg caattggaaa agttaattcc
    15001 tctttgttat tgccgttgcc tccagagttg tttccagagg tatctgtacc tccgcccccg
    15061 catgctgcaa gaacgacagc agacatcagc gctgcaaatc caaaaatcgt tttcttcatt
    15121 cctatttccc ccttcgcttc tatactagcc taaccgttgg cgcgcatctg cagaacgctt
    15181 gaatgcttgt ccaacataat tgatactcaa catcaaaact aaaataagga ttgatgcggg
    15241 taaccagatc cattgccgat tcactaagac ttctccgtta cttgcataac cgatcaatgt
    15301 acctaaacta ggtacatttg ccggcaagcc gaaacctaag aaagtcaaag ttgtctcgat
    15361 accgatgttt gccgcaaagt tcatcgtcaa attagtaatg attaatgaac ttagattcgg
    15421 catgatttcg cggaacatga ttttaaaatc attggttccc attgtttttg aggcactgac
    15481 ataatctctt cttgcttcag ataatgtctt gcttcggaat aatcgggctt tagctaccca
    15541 ataaaatgca ctcatgatga atataaagga ccagacattg tatctggaaa taatcgatac
    15601 gaagacgata atgatcagca tgatcggtag aatcatgata aaatcaacga tccgcatcaa
    15661 gatgttgtca atgatccctc cgtagtagcc agagatgatc cctaatccaa ccccgatgat
    15721 cgaagtaagg atcgtgatcg caaatccgat cgtaacagag tttctagcac ctatgatcaa
    15781 ttgtcctaat acatcacgtc cgccttcatc tgctcccaga atgaactttt gtccttgtgt
    15841 cgaaactgtt cctggtgcag cgtatttgtc gaagatacta acatacatca ctttgtcttg
    15901 atcggtcagc atcgcaccga taaagatcac taataataaa accactagca aacctaatga
    15961 aaaaatagca attttatctt ttttaaattc tcgagcaatc attcgtatcc ccataggagg
    16021 aatactttct tgaactgctg tattgttttt ttcactcatc gatccattct cctttctgat
    16081 tggttttatt gaatccgaat acgcgggtca acgatactca tgatgatatc tgaaagtaat
    16141 gtccccacta gcgtcgcgat tcctaaaatc aatactaagg atgtgatcac ggtatagtcc
    16201 cgttggccga tggaatcaat aaatagtttt ccgactcccg gataaccaaa aatcttttcg
    16261 ataactacag aaccagcaat caacgaagtg atttcatacc ccatttgaga agccatcggc
    16321 aacgctgcat ttcggaaaat atgtcgactg aatactttat tcgtcggtac ccctttggaa
    16381 cgggctgttc tgacaaaatc gagcgatttc gaatcgatta cttcacttcg cagatattgg
    16441 attgtgacgg cagtcgccaa aaatgcttgg gtaattgatg gtaacagaat atatttcaaa
    16501 cggtctatcc attgagtgat aaatccacct tgcaaaccta gtgtttgtga tccacttgtt
    16561 gggaaccagc ctaaacgata accaaaaaca aatagcatga tcaagccgaa gataaataaa
    16621 ggaacagcaa aactaaagaa gttgtagatt acgacaatct tatccaagat tgaattttga
    16681 taccggccag caagcatccc cagaggcaca gcaatcaaat aagtaaggac aactgtcaag
    16741 atcgatagat ataaagtgtt gatgatccgt tcgccgatca agtcagcaac agagcgtttg
    16801 tataagaaac tttgaccaaa atcgccttgc aaagcatttc cgatccaacg gaagtactgg
    16861 acataccatg ggtcattcaa gccagctgct tgtcgcatct gttcgatcac tgccggatcc
    16921 tgatttggat tgatcaaacc ggtaaatgga tcccccggca tcgcttgggc taataagaag
    16981 atcagaacac ttaaaataat aacttgcggg atcatcaata aaacacgtcg cagaatcgtt
    17041 ttccacatta gcttgcacct ccatctttca acgccacttt gtgtgtagtt gtgatcgtac
    17101 gcaaatcgta gactcgacca gtcgcatcat agtattcttt ttggttttgg atatactctt
    17161 gttctactct tcgtcgattt tctttgtggc tttccctatt ttctacatcg atttgcggaa
    17221 tagctgaaag cagacgtttc gtatagatat gtcgcgggtc attataaatg tcttctcttg
    17281 taccgatttc tacaaatcga cctttgtaca taattgcgat attgtcgcac atgtgtttta
    17341 ctacacctaa atcatgtgaa atgaataaat agctgaggcc gtattcttgc tggattcgtt
    17401 tcatgaaatt caatacttgc gcttgaacag atagatccag agcagataca ggttcatcgg
    17461 ctacgatcag ttttggattg gtagcaacag cacgggctac tcctagacgt tgacgttgtc
    17521 cgcctgaaaa ttcatgagga tatttatata gcgcatcttc cggcatccca acgatatcta
    17581 gcagtccttt tacctttttc ttttcttctt gatcgcttaa ccgttcaaaa tttcggatgg
    17641 gttcagcaat gatatccaac actcgctttt taggattcaa actggacatt gaatcttgga
    17701 agatcatctg cacatccttg ttataaccca tttttttgcg gttgcttcgc ttcgttacat
    17761 cttttccttc atacatgatt tgtccggctg ttaccttttc taatcccaca acggcttttc
    17821 ccgtagtaga ttttccagag ccagactctc ccactaaacc atatgtcttt cctttttcga
    17881 taataaaatc cacaccatct acagcatata catgatccgt tactcgattg aagaatccac
    17941 tgcgaatagg gtaatgaact ttcaggtctt tgattgtgat taattctgtc atctacacct
    18001 ctcctttttc gtctctaaag tggaaatgct tatagcagga gcaacgtacc aagtggttcg
    18061 gtgcgacttc atgaaggata ggctcttctt catgatcgct tgctggtatc catggaatac
    18121 gtggtgcaaa ccgacaacct ttacgaggca tgtttttcag agaaggaacg acaccttcga
    18181 tcacatggag ttctgcatca tgatttcctt cttgtggtat agagttcagt aaagagcgtg
    18241 tataaggatg cttaggatga tcaaacagtt cttttacacc tgctacttca acgaactgtc
    18301 cgccatacat cactgccact cgatcagcca tttctgctac gactcctaag tcatgagtga
    18361 tcaagatgat ccctgttttt gtttcttcct gaaggtcttt cagtaaatca agaatctgtg
    18421 cttggatggt tacatctagg gctgtcgtcg gttcatctgc aatgacgatt ggaggtttgc
    18481 aggcgattgc gattgcgatg atgacacgtt gtctcatacc gcctgatagt tcatggggat
    18541 attggcgtcc aactctcgca ggattaggaa tccctacttg ttctagcaat tctagtgcac
    18601 gagcttgacg ctgttctgca ttcatctttg tgtgatagga aagtccttct ttgatctggt
    18661 cttcgattct catgagagga tttaatgcag acaacggatc ttggaaaatc attccgatat
    18721 catttccgcg gattttatta tagagcgttt cgttcaggtc gattaaattc agatcgttat
    18781 ataaaatctc acctgtaatt tttgtgttat taggatcaag taatcccata atcgtagtag
    18841 ccaaggtact tttcccgcac ccagattccc caacgattgc taaaatctcg tttttgtcta
    18901 aagtaaatga aacgtcatct actgcatcgt aatagtcatc tttgagtcgg aatcctgtat
    18961 gtaggttctg aacttctaat aattggttat cggacaatcg aatacccctc tctgcttcct
    19021 aaatggaagt ttttcttaat aaaaaaggac attacacaga ata
//
LOCUS       DADXTG010000046        19041 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.047, whole genome shotgun sequence.
ACCESSION   DADXTG010000046 DADXTG010000000
VERSION     DADXTG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.047"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            173..487
                     /locus_tag="IWI57_001366"
     CDS             173..487
                     /locus_tag="IWI57_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBC2449518.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            465..2159
                     /locus_tag="IWI57_001367"
     CDS             465..2159
                     /locus_tag="IWI57_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBC2449519.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2179..3357
                     /locus_tag="IWI57_001368"
     CDS             2179..3357
                     /locus_tag="IWI57_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBC2449520.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKDFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            3320..4006
                     /locus_tag="IWI57_001369"
     CDS             3320..4006
                     /locus_tag="IWI57_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBC2449521.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            4006..5166
                     /locus_tag="IWI57_001370"
     CDS             4006..5166
                     /locus_tag="IWI57_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBC2449522.1"
                     /translation="MTIKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5176..6051
                     /locus_tag="IWI57_001371"
     CDS             5176..6051
                     /locus_tag="IWI57_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449523.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6048..6359
                     /locus_tag="IWI57_001372"
     CDS             6048..6359
                     /locus_tag="IWI57_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449524.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            6349..6702
                     /locus_tag="IWI57_001373"
     CDS             6349..6702
                     /locus_tag="IWI57_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBC2449525.1"
                     /translation="MANNRRLEETFNDGWLKILTQSTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            6692..7093
                     /locus_tag="IWI57_001374"
     CDS             6692..7093
                     /locus_tag="IWI57_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449526.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7086..7490
                     /locus_tag="IWI57_001375"
     CDS             7086..7490
                     /locus_tag="IWI57_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449527.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            7502..8110
                     /locus_tag="IWI57_001376"
     CDS             7502..8110
                     /locus_tag="IWI57_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2449528.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            8130..8492
                     /locus_tag="IWI57_001377"
     CDS             8130..8492
                     /locus_tag="IWI57_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449529.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            8495..8677
                     /locus_tag="IWI57_001378"
     CDS             8495..8677
                     /locus_tag="IWI57_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449530.1"
                     /translation="MPNLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            8694..12014
                     /locus_tag="IWI57_001379"
     CDS             8694..12014
                     /locus_tag="IWI57_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2449531.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALDMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEKFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFKGIWSKF
                     TDWLGGTWESIKEGASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPHFKLSGKFNPLKGKIPTLGVDWYAKGSVFNSPNIIGIGEAG
                     PEVALPLKRSVLQEIGDRILSSTSVSSRAQTVQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            12065..12802
                     /locus_tag="IWI57_001380"
     CDS             12065..12802
                     /locus_tag="IWI57_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2449532.1"
                     /translation="MTDCIHSIVDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            12812..15103
                     /locus_tag="IWI57_001381"
     CDS             12812..15103
                     /locus_tag="IWI57_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2449533.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIIEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWILVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            15127..17205
                     /locus_tag="IWI57_001382"
     CDS             15127..17205
                     /locus_tag="IWI57_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449534.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVITLDPNHKLEVLKPKSQPALLTGKTYTVSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPFQPNLLAEPYNMCREYPNENIADPTVKFPIESGDH
                     QIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTF
                     TPTNVVAMPKLFRVYQYPRSTVGACRIDWLKIEKGNTRTPNISEYKYRGTGMRDSNNP
                     KDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDA
                     QTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNG
                     KRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     DYTFAITYTI"
     gene            17222..17371
                     /locus_tag="IWI57_001383"
     CDS             17222..17371
                     /locus_tag="IWI57_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449535.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            17368..17814
                     /locus_tag="IWI57_001384"
     CDS             17368..17814
                     /locus_tag="IWI57_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449536.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            17816..17953
                     /locus_tag="IWI57_001385"
     CDS             17816..17953
                     /locus_tag="IWI57_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBC2449537.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            17991..18284
                     /locus_tag="IWI57_001386"
     CDS             17991..18284
                     /locus_tag="IWI57_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449538.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            18281..18505
                     /locus_tag="IWI57_001387"
     CDS             18281..18505
                     /locus_tag="IWI57_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBC2449539.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            18502..>19041
                     /locus_tag="IWI57_001388"
     CDS             18502..>19041
                     /locus_tag="IWI57_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBC2449540.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLS"
BASE COUNT         6715 a         2898 c         4020 g         5408 t
ORIGIN      
        1 ttggatatag cattgaagag attaattatt acattgactt cgctgaagct ttcattccga
       61 tggtgagata acccccctta aaataaatcg caaatttttt gggggtgatg aaacggaggg
      121 ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta aaatgtttaa
      181 aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa taagccaatt
      241 ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt atatcagttt
      301 atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta gactggagaa
      361 tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa tgggattgat
      421 gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat caattcctga
      481 gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc aaggtatgca
      541 aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga gaagatattt
      601 acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac tttccattcg
      661 aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga tggaaaaaag
      721 ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat ggtaaaactg
      781 gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt aaaaattatg
      841 atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat gatgtattta
      901 aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg aaggaagtta
      961 ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct agaacaaaag
     1021 atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa gattattcaa
     1081 tgataaatgt ggctacttct ggtggtggta aaattcgaga ttatagagaa ttttatgata
     1141 caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa tcaaaaatga
     1201 ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt ttgtttccat
     1261 ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg gaaaaagctt
     1321 gtccaactat taattacaat gcagatttaa aacggaaaat gtttcaagaa tactctcaaa
     1381 tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac agacctatgg
     1441 aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa gaaaaagaat
     1501 ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga cgagattttg
     1561 ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa catactttta
     1621 tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat atttctatag
     1681 atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat attgtaggtt
     1741 ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat atgtaccgtg
     1801 caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt gttcgaagcg
     1861 gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt aatcaacgtt
     1921 tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat gaagagcata
     1981 tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa acggatggct
     2041 ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct gctcctgtaa
     2101 cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta gtattctaac
     2161 ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt cacaaaaaaa
     2221 gcaacggttc ctttagaaga atacttttgt aaattgcaag ttgattttgt gtatcgaaaa
     2281 tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc ggaattcaag
     2341 tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa tgtagctcct
     2401 aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt aatattctac
     2461 aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat tgcggatagt
     2521 tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca aattaacaac
     2581 atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata cgcagatcaa
     2641 caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat ttctagtgcc
     2701 atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa tttattccga
     2761 ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca atttaaggat
     2821 tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta cacattagaa
     2881 gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat agacgacatc
     2941 tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg agacatgagt
     3001 gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat tgctgaactt
     3061 attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc agggaatttt
     3121 gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt tgacgctatt
     3181 gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagaacg cgttggaaat
     3241 gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa tcaacaagta
     3301 tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa agaatgaagg
     3361 cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct ttgaagggaa
     3421 tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag aaattgaagt
     3481 agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga acttacttaa
     3541 gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg ctgcttcaat
     3601 tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga tgattcaccg
     3661 cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg accaactgga
     3721 atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca cagatgaagc
     3781 attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt tgagttatgg
     3841 attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta aaaaagaaca
     3901 aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa ttgctgcatt
     3961 tacagcagca ttaaataaaa catttggaca aggagatgca aaataatgac aattaaaaat
     4021 ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga tggtaacgaa
     4081 gaatctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa aattttagaa
     4141 gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca gcgtgtatta
     4201 actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt tgcaggggtc
     4261 gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga acaatctcat
     4321 ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg gattgtgtca
     4381 cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa agttttagat
     4441 aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat tcctgtatgt
     4501 aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac tgttttagta
     4561 gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa agatatgcca
     4621 gtcggaatga tgcgtgacat gagcaaacaa actagcggag aatatgctga aaaaaaagca
     4681 gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg actatcaaaa
     4741 ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc tgatgtggtc
     4801 ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac tgtactaact
     4861 gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca gtcaacggct
     4921 gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg tgtaggatct
     4981 acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg catttatcta
     5041 gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt attagatatt
     5101 agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac tacaacacaa
     5161 gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg gtttaaatgg
     5221 gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat cgtattgctt
     5281 ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg tgttgaagtt
     5341 catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa accatattta
     5401 tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt ctatatcgat
     5461 tcggatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa agatgatgtt
     5521 tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag ctgtgaaaac
     5581 ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga atctttggaa
     5641 actataaaca ctaattcagg aggcgcacag tgggttatta accgtcctaa agcgaattat
     5701 tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg gcaaaaaaca
     5761 aatatacaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat gtatttcaat
     5821 attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc aatagaaaaa
     5881 gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga agatttattt
     5941 ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt tgtaaacaag
     6001 aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg acgcctgaac
     6061 aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc tggaacgaag
     6121 aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa agtaaagtga
     6181 gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc gaaagatgta
     6241 gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt gagttattag
     6301 gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat ggcgaataat
     6361 cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca aagtaccaaa
     6421 agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc tttaaaattt
     6481 agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg atcgaaatta
     6541 actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa agatcaatat
     6601 tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga taatttttat
     6661 atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta aagaaaaaga
     6721 acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt ttggcctcaa
     6781 actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt acttcattct
     6841 cgaaacagga gaaatagaaa tgatcactga gccaaaatat agcgtgggtc aaaatctata
     6901 tctaactttc tattcagaaa atagagaaga tttaacagga gactcactag atattatttc
     6961 attgattcaa aatcgttcga ttcgttttca gagaatggat cccaatcatt taaaactaga
     7021 gaaccaagat cgctatatcg atcaattggt atttacgttt agacgattat tgaagagtga
     7081 ttgtcatggc taaaaatagt tgggagctaa aaataaatgg acatgatgaa cttcttgtgc
     7141 ggatggaacg ctattcaagc gagagcgaac gactgattaa cgaagcattg aaatcaaaag
     7201 gttcggctat tgcagtggat aggattacag aaaaaattcc tgtttctgaa gcagatttaa
     7261 gaagagggca ccaacacgca aaaaatagtc gtccacttaa gactcaatac attaatttgg
     7321 gtttcatcat tagacctaca agaaaatttg agtatttaaa atatcctgat ttggggatag
     7381 gtacttctaa aagaaatcag ccggacgaat ttatgagaag aggattaggt cttgcacttg
     7441 atccaattac agaacttctg attcgtcaat tcgataaatt aaataaatag ggggaacaac
     7501 aatggctaaa acaaaaactg tagtaacaac gttcgataac gtaagtatca aacgaattgc
     7561 ttttaatttt aagaacgcag gaaatgcaat cgcaacgaat tgtaacggac aattagatgg
     7621 cgaaacagaa atgcaaacgg tggttaaaaa atgtggagcg acagaagtaa aatcaaaatc
     7681 taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag tttatcgacg
     7741 tttcaatggg ttgaaacaag atggacgtat taaaccaggc atttactctt acggtcctga
     7801 ttccgtaggc gaagatttct cacttgctgc agagatcgtg gatgacttcg aagaaaatag
     7861 aaagttagtt ggtatgttag catgcacttc gaatacagga ttaacattct ctattgaaaa
     7921 tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag atgaatttgg
     7981 taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag atcaatggat
     8041 gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta cggccactca
     8101 atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa attgaactaa
     8161 acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta ttgatgatca
     8221 atagagattt taacacagac gagtttgcaa aaatgtcgat gggaaaaggc tctatggaca
     8281 ttacagttat tcaaggtgca aaagccgtat atgtagctta tcgtcaagcg aatatggttg
     8341 attacatttc attcgatgaa tttatcgaca aatgggattt tgatatggag gttgcagtag
     8401 ctgtatacag tactatgatg ttcaaacaag cacgagatgc ctatcaaaaa gaattcgaaa
     8461 aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aatctcttag ttgaaacgtg
     8521 ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt tggttttaag
     8581 cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagtgctt atgaaggatg
     8641 gaaaaactat gcaataaacc aatcccagaa aaactaaaga aaggaggtaa aaaatggcta
     8701 aaaagagaac agaagcagaa gtaacattta tagctaacga tgacggattg aaatctacgt
     8761 taaaagaaat cagcgctgaa ttaactaaaa atagagcaga attaaaacta gaacaagctc
     8821 aattacaaca gactggttct gaatcagaca agttaggaag taaattatct tctttagaga
     8881 agcagtatga attacaaagt caaaaagttg aagtaactag tcaacgttta gccaatgcga
     8941 aaaaatatta tggagaaaat tccaccgaag ttcagaaact tgagagagaa ctgattaatc
     9001 aacaaacagc gcaacaaagg ttatctaatg atatcgataa aacgagtaaa gcattggctc
     9061 aatctaaagg tgaatttaaa acctatgagt caactatgaa ggatttagat aatgagcaac
     9121 aacacctaaa atcaagtgca tcattagtag aatccgaata caaaaaatgg caagcaactg
     9181 ctggtcagtc tgctaccgaa tctgaaaaat tagcaaaagc acaagaatat gtcggcaaac
     9241 agagtgatat tgctgaacaa aaaatcgaag tgctaaaaaa acaattagat gccacacaaa
     9301 aagaatttgg agaaacatct accgaagctt tagatatgaa aactaagctt aatgatgcgg
     9361 aaagagaatt cgaagaatta agtaatgctg ctaaaaacgt tgatacatcc acagtagacg
     9421 atatcggcaa aaaacttgat atgggcaatt taatggaagc tactgatcac ttgtcagtga
     9481 tcggagataa gctgattgat gtaggcagta agtctattga agcagctgga aaagcacaag
     9541 ctatgcaggc ccaatttaaa caagtctttg gctctttaga aggggaagcg caggacgccg
     9601 ttgagggaat ggctgaaaaa tttggaatgt taccaaatac gatcaagcct gtttttacgc
     9661 aatatacgtc aatgtttaaa ggacttggat atgatacaaa agaagctatg gagttagctg
     9721 gtgacagtac tcagttagca gcagatgcag cagcttttta cgataagtct atggatgatg
     9781 ctagtgaatc tcttaattca ttcataaaag ggaactacga aggtggagaa caaataggtc
     9841 tatttgctaa tgatactcaa atggctgctt atgctgttaa gcataacttg ataccagcga
     9901 ctgaaggagc aaaaaaagcc agtgaagagt cattgttagc cgttgaaaaa gcacaatcta
     9961 agtatgctga tgctattaag aaacatggcg aaggatcttt agaagcaaga gaggctgctt
    10021 taaaacttaa agatgcgcaa gataaaataa atgaagaatt aggcccacaa actcaaaaat
    10081 ggtctgattt agatgaggct accaaacaag cagttcgagt tcaatatgct gaagatatgc
    10141 aaaaattagc cggtgctaca ggtcaggcta gtagagagtc tgatggttta gaaaaccaaa
    10201 tgacaagagc aaagcaagca ttagaagatt tttatgcctc gttgggtgaa gatatattgc
    10261 ctgtgtttat caaagggtta caagcaggcg cgaaagcttt gcaaggatta gccgaatggt
    10321 ggagtaaact tgatggacca atgaaaaatt tcattttagc tcttggagga attctagcat
    10381 tattaagcac attagctcct gttataaccg cagttgttac gatagttggc acatttggtt
    10441 ctacagtttt gctgccaata ataggaatta tagcgggcgt tgcagctgta atagcgattg
    10501 taatcacagc gtttcagaac tggggtgcaa ttactgactg gtttaaggga atatggagta
    10561 aattcactga ttggttgggt ggtacttggg aaagcataaa agaaggtgcc tcatcagttt
    10621 gggatggagt taaagaaacc tggtctggat ttgtagattg ggttcaagaa atttggcaag
    10681 gagtttctga ttggtttgga gagttatgga gcggattagt tgaaggagct tccaacatct
    10741 ggcaaggagt ccaagagact tggcaagcat tcgttgattg ggtttcaaat atttggaacg
    10801 gagtcaaaga agtatggtcg attatttggg cagacattgt aggaattgtt caaataccat
    10861 ggacattaat aacgtcattg attcaagctg gtattaatat tatcgtgggt atctttgatg
    10921 tagctggaca gttattaggc gcagcttggc aagctgtttg gacacctatt tctgatttcc
    10981 ttaaaaatac ttgggatact atgacacaat ggataagcat cgcttggaat ggaattgtaa
    11041 ctacattcca tactatattt gatccagtag tggcatggtg gaatggtata tggacatcta
    11101 ttagtactac ggcttcaaat atttggaact caattagtgc aacagcttct agtatttgga
    11161 atagtatcaa gaatacaatc actagcttgg tacaagcagc tgctacagta attcaaaata
    11221 tttggtcaac tgtatctagt tggttaggtg gaatttggaa ttcaatcagc tctacagcat
    11281 caaatatctg gagtagcgtg actagtagta taagcaatgc tataaacgca gctaaaagtg
    11341 ccattcaaag tgtttggaat agtatatctt cgtggatcag cggaatttgg aacggtatca
    11401 aaaacactgc tttgaatctt tggaatggaa ttacaagcac tattagctct aaagtaaacg
    11461 atggaaaaaa tgcaatttca agcggttggt ccaatctaac aggtattgtt tccgacatat
    11521 tcaataatgt taaaagtacg attgctaaca tttgggaagg tattaaaaag actgttagcg
    11581 ctccgattga ttggattaga gataaaatca gtggcatctt tgataatctg aatatttcga
    11641 taccacatat tccgttacca cattttaaat tgagtgggaa attcaatcca ttgaagggaa
    11701 aaatcccaac gttgggtgtt gattggtatg cgaaaggtag tgtgtttaat tctccgaata
    11761 ttatcggtat cggtgaagca ggacctgaag tagctttacc tttgaaaaga tctgtgctgc
    11821 aagaaattgg ggatcgtatc ttgagtagca cctcagtttc atctagggca caaacggttc
    11881 aacctgtgaa caattacgaa ttcaatttca caattgatgg taacgcagat gaggttacta
    11941 tgaagcagac aactcaacaa atcattgaca gcattacaaa agttcaaaat gataatgctt
    12001 cggcatggcg ttaaacagga gagtatttct cctgttttct tagtattaca aaggatgtga
    12061 aaaaatgact gattgtatac attctatagt cgatggattt cctgattatt tgcataaatt
    12121 ggctttagct gaaagaccaa ccataccttc tccaaaaaga cagagagttg aaacttctgt
    12181 tttaggacgg ctaggtggct tagtacaaga ttattcgttt gaagatatgt cgtttacttt
    12241 gcactataac tatttagagg atgtggaaga ccatcaagcg ttcaagcaat cgttttatat
    12301 catgcgtcat tggttaaatt atgcaaagaa attagaattc tctgatgatc ccaacgtcta
    12361 ttatgttatc cagactatcg atattgggga tgcagaaaac gatattgttg aatggggaga
    12421 gttcgatgta aatatcactg cgaaaccatt cgcaagagtt caagaagatg tacctataac
    12481 cgtagataaa ccacagtcat ttaacttgct gaataatagt ttagaagaaa gttttccaaa
    12541 gattatcatc actccttcag ctacttcatg ccaattcatc ttaaatgatt atgtgtttag
    12601 ttttgaaggc ttagtagtag gaactgacat agtcattgat agtgatctaa tgctttgcta
    12661 cgaagagcaa tcggacggag atattttaga tcggtccaac aaaatgaaga ccatgcaata
    12721 tccgacattg caagtggata tcaatcattt taattgcact ggtttgagca aaatacaaat
    12781 ttatcgtaat gggttaaggt aggtgaaata gatgatcgat aatttaataa ctatttacga
    12841 taaaaatgac gcgaataatt tagctgaaca tttatatgat acgcaaggtt taggcgcttt
    12901 gtcagactgg ttaacagcca ctgttagcaa taaactaaac ggagccgaga tatttcaggg
    12961 tacttatcca ataagcggaa ctaatgcaga tttgattata gaaggacgta ttattcagtg
    13021 ctatgtagat gaaaatcgag caaagcaacg tttacggatt tattatgcaa agacttctgt
    13081 aataggaaat acgatagaag taaaagctga acctattttc aatgatataa gaaaatcggt
    13141 gttgaataaa tatgacagtg gaacagaaaa gatcactgct actcaggcat ggcaaaacgc
    13201 aaaagcttta gcgaaaccag ctatcccttc gcagttttct ttctcgtcat tagtagatac
    13261 gcttgctaat gtgaagatag aaaaagcgaa ttttttagaa ttctttggtg gaaaagaggg
    13321 atctattcta gatcgatttc atggggaatt tcttaaagat aataacacat tacgtcatga
    13381 aaaaaggcta ggtacggatc ataaaatcaa agcgatttat actaaaaatt taactggtct
    13441 tgacttagag atagatgctc aaagcgtttt agttggagtt tatccattca ttagcagctc
    13501 ttcagaagga gaagacgaga tcactctacc agaagaagtt attttcacgg attacgtgga
    13561 tgattatcct gctggatatg tttcttttgt tgattttaaa gacaaagcga ctgatgtagc
    13621 cacattaagg gaagctgcta aagactggtt gaaaacaaac atagataaac aaaaaccaca
    13681 agtgagtggt tcgattgaat tagtaccatt gaggcatcaa agaggctatg aaaaatttgt
    13741 tgatctagaa aaagtttcga tgggtgacgg agtagatgtg tatcatccac agttaaaagt
    13801 gaatatgtca gcgagaattg tggaatatac gtttaatgtt ctaaccaatt catacgataa
    13861 attagttgta ggaaacgtca aaacaaactt cttagaaaac acagagaata atgtcagcaa
    13921 tttgattaat gatgccattg atcaattgaa aaacggtggc gaaatcagtg atttaatcaa
    13981 tgatattgta gatcatcaaa ctgatatgat tactggtcaa aatggtggtt atgttttatt
    14041 agatcctaaa gaagcgccta gtcgtatttt gattatggac acaccagata agaataccgc
    14101 aaggaatgtt ttacaaatca acaatgctgg tattggtttt tctaaaactg gcattaatgg
    14161 aacatatgaa acggcatgga cgttagatgg cggattcaat gcctcgttta ttacggctgg
    14221 tgaaatagta ggaattacta ttagaggtac tacattaatt agtgatggtg ctgattatag
    14281 aacaagtatt gctaatggca aaatgacttg gtactcaaaa aaagttaaca aagatattat
    14341 ggagctagaa gcacgtgatt atgtaagtgc tgatgccggt attgtatcat acaccatgaa
    14401 aactggtggt ggtttcatga ttagaaatcc acagggtaac ttggttttta gtacgtggga
    14461 taatggtaat aacagaccgt ttctatcttt tggtgcgccc aatttcaggt atagcaatgc
    14521 tagttatgta acttctggcg acggtagttc tttaagcatt aatggtagtg cgggtaactc
    14581 atgggaattt aaggtagctg gtaggactat gaaatttact agtgatggta tgctaacgtt
    14641 accaggttgt ttttttggtt catgggaaga tgggaaactt gctaggtttg aacaatcaac
    14701 ggtacaagta tataaagatt ttactgttag aggtactaaa aactcaactg taccaacaga
    14761 acattatgga caacgactat tgaacgctta tgaaactcca gaatattatt tcgctgatta
    14821 tggggaagcc gttacaggtg acgatggtaa agttcgtgtt gatattgacc ccatgtttgc
    14881 tgagacagta aatctaagtc ggtatatgac acatgtgaca cctacagaac tagttttgtg
    14941 tgctgttact catgaagata ttgaccattt catcattgaa actagtaagc caaacgtatt
    15001 agttagatgg attttagtgg cacaccgtct agggtatgaa gatattagat taaaagagga
    15061 tacagcatat gatagcacag tgcttgacca aaaacgtttt taaaacgaag acaaggaggt
    15121 atataaatgg ctagcagttt atataatttg gctttagatt tcagcaaaga attaaactac
    15181 accaaagcta ttatggctcg tcagggtgat aaagggatta cggtgacggt taaaccatat
    15241 ttaaatggct tgcagatgga tacgagtggc ggaacattta ctttaaaagg aacaacacca
    15301 tctaaccgtt acgtagatag tgttgcaact agcgtaacta gtgaagaagt cacgttttct
    15361 cttgatggca catttatgag tgaagcagga tattataaac actgctacgt agaatataga
    15421 aaagacaatc aaattttaac aacgcaagat atcatttttt tctcactagg agtgtctgac
    15481 atttcgcaag gtcaagccga tgaatatgtt tcgcaattag aagagttgat tcgaaagtat
    15541 aatgaaactt ttgatgcttt tatggctgaa actaaaggta gagtggatag cttaaatcaa
    15601 cagattactg atttaactgg tcaagctaaa acgctacaag acaagttaga tgctctgaaa
    15661 gaagaaattt ctaagttagg taacttacaa gtgatgtaca gtaacagcat tgatttcggg
    15721 ggctatgatt attcggggaa tccgaatttg atggctaata taaacgctga tagcttctcg
    15781 caaggtagtg gtgccttatc tgttgtagat gatggtgacg aggtagtgat tacgcttgat
    15841 ccaaaccata aattagaggt attaaaaccg aaaagtcaac cagccctatt aacaggcaaa
    15901 acatataccg tgagtgtaga aattatgtta gaaggtgatt tcactggaga ccctagcaag
    15961 ataggcctaa gatatattaa aatgcctaac tgggtatcag agctatatac gcgtaataca
    16021 ttaactgcta ctaagggtgt atggcaaaaa ctaactggca ccgttaaaat tactgctgcg
    16081 agtgataacg ctgaaagctg gcttataatg ctacaaaata aagacgctaa taacagccta
    16141 tccggcaaac tacgtttgag acacgctaaa ctcgaagaag gctcaacagc tacaccattc
    16201 caacctaact tattagcaga accttacaac atgtgtcgcg aatatcctaa cgaaaatatt
    16261 gccgatccta cagttaagtt cccaatcgaa tctggcgacc accaaatata tcaaggttac
    16321 acagaagaag agcttatgat aggtcaaacg tatactatca cgcttaaagg aacaaaaccc
    16381 gcaagtcaaa cctttgtagc gtataatcat tggactgctc gtttaggaga actaaagccg
    16441 gttgatgggt tgacagacgt atggtctcta acattcacac caacgaatgt tgtggcgatg
    16501 cctaaacttt tccgtgttta tcagtatcca cgatcaacag taggcgcatg ccggatcgac
    16561 tggctcaaga tcgaaaaagg caacactcga accccgaata ttagtgagta taaatatcgt
    16621 ggtactggta tgcgtgattc aaacaatcca aaagattatg tttgggatct agcaccagaa
    16681 tatgtcgaag ataatttggc cacagatatt aaaatttctg aaattactgg taaagcaaac
    16741 aattataccg atgggaaagt atcggagatt aattcgcagt tgactgcttc aattaatgaa
    16801 gtagacacca cagctaagga tgctcaaaca aaagcgaatg ctaatgcgac tgctatagat
    16861 gaattagaca ataagatcga tgaacgcatt aatgatacag ctactaccac attaacagtt
    16921 acaaacggga ataccggatc agcaaagctt tatcgtgaag gaaaaacagt ttctatatat
    16981 tttgtggctt taaacggaaa acgcagtggt ggaaatgatt caacgatact aacaattcca
    17041 gaaggctatc ggccaccaat tagttttgag caactggttg gctcgataga ccgttctact
    17101 ttgaacagtg ctcagttatc tattggtgca gatggagcca ttaaatggcg aagaaactca
    17161 agttatggat cggattatac ctttgcaatt acttacacga tttagaaaag cgtgaatcga
    17221 tatgaaggca gcatatagac caattgaacc ttacggattc gagcaaatca ttgtgaatga
    17281 tgaagaacat ttaccggaag aatgcacaga agtcgaacca ccgattccaa attggaaacc
    17341 gaaattcaat tactgggagg gaaataaatg aaaaacattt ggaaatatgg acgtactggc
    17401 ggagagtacg caggaaaagt attggacgac atgcttgtat ccgttcctta cacagatcag
    17461 ccaccgcttg aaggaattcg tgctgatggc gaaccgttaa caatcgctga tcaaatgttt
    17521 gatcctaaat tgaatcaatg gattatttta gcgaacgcac tagatcacaa cgatttaaac
    17581 aatctcaaag caatgtatga gtcgttagaa aatgagaacg gcgatttaaa acagatcaat
    17641 gccaaactca tgctaagcga tgtagcaatt aaacaggaaa atactgcatt gaaagaaaaa
    17701 gcggatagtt tagcacaaat caattcaaaa atgatgcttg cttcgttaca aaatagcaaa
    17761 gacatttcag aaattaaaga gcaactaaat ccagcttcaa agggaggtga gtagtatgtt
    17821 tagttttagc gatgtgaaaa tgatgtatga ttggggctgt ttcactaacg aacaagtaat
    17881 ggttttcgtt ccgttgtgca ttactgaaga aaaagcagat aaaatcatta gcaaagaaga
    17941 gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac atgattaatt
    18001 taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg attttattag
    18061 taataaaacc aattactgca tctttctcga agattactga gactctttca aaagtaagtc
    18121 acaatttaga tttgctgact aaagatttag aatcgagcaa atcagatcga ttgatgattc
    18181 atgaagaact aaagaaacac gatgaaagat tagatacaca tgcagaaaaa ttggtagaac
    18241 acacgcaaca aattaaaact ttatttaggg agagaagaaa atgaataata aaacgttcga
    18301 agtactaaaa tggttcgcac tggtaattat tcccgcacta gctactttcg tggggttagt
    18361 tggtaaagcg ctcaattggc agtacacaga tatctgtgtt gtcatcatta ctggttttgg
    18421 cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc tacaaaatgt tctcggctga
    18481 aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta ttactgcgat gagcctgtta
    18541 acggctcttt ttttattacc cattaatacg tttgcttata ctattaatga cgagtataat
    18601 ttagcgccga atcaaggaga ctccagatta gcaattccta acaaaattat tttgcacgaa
    18661 actggaatag atgcaccagc aagaaacgta gccgccaaca tgaaaaataa ttataacgga
    18721 agcaattctt atactacaga tgttattggt gacggtggga ttgtttaccg tgtgggtgag
    18781 caaggatatg tttcgtgggg agctggtaac gctaatcctt atgcgcctgt acagattgaa
    18841 ttacagcgca catatgataa agcattgttt gaaaaaaact atcgagctta tattgaatat
    18901 acaagagata gtgcaaaaaa atatggaatt ccattgactc ttgatcaagg aacttcttta
    18961 tttacaaaag gaatcatttc tcatttgtgg gtgacaaatt atgtttgggg gaaccacaca
    19021 gatccatatg gttacttatc g
//
LOCUS       DADXTG010000047        18691 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.048, whole genome shotgun sequence.
ACCESSION   DADXTG010000047 DADXTG010000000
VERSION     DADXTG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.048"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1130
                     /locus_tag="IWI57_001389"
     CDS             138..1130
                     /locus_tag="IWI57_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2449541.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            1182..2051
                     /locus_tag="IWI57_001390"
     CDS             1182..2051
                     /locus_tag="IWI57_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2449542.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(2471..4036)
                     /gene="guaA"
                     /locus_tag="IWI57_001391"
     CDS             complement(2471..4036)
                     /gene="guaA"
                     /locus_tag="IWI57_001391"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBC2449543.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(4095..5018)
                     /locus_tag="IWI57_001392"
     CDS             complement(4095..5018)
                     /locus_tag="IWI57_001392"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBC2449544.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            5214..5819
                     /locus_tag="IWI57_001393"
     CDS             5214..5819
                     /locus_tag="IWI57_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2449545.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            6127..7143
                     /locus_tag="IWI57_001394"
     CDS             6127..7143
                     /locus_tag="IWI57_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBC2449546.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            7284..8585
                     /locus_tag="IWI57_001395"
     CDS             7284..8585
                     /locus_tag="IWI57_001395"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBC2449547.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            8631..9293
                     /gene="deoC"
                     /locus_tag="IWI57_001396"
     CDS             8631..9293
                     /gene="deoC"
                     /locus_tag="IWI57_001396"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBC2449548.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            9314..9712
                     /locus_tag="IWI57_001397"
     CDS             9314..9712
                     /locus_tag="IWI57_001397"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBC2449549.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            9778..10860
                     /locus_tag="IWI57_001398"
     CDS             9778..10860
                     /locus_tag="IWI57_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2449550.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            11072..12634
                     /locus_tag="IWI57_001399"
     CDS             11072..12634
                     /locus_tag="IWI57_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449551.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            12627..13760
                     /locus_tag="IWI57_001400"
     CDS             12627..13760
                     /locus_tag="IWI57_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449552.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            13760..14716
                     /locus_tag="IWI57_001401"
     CDS             13760..14716
                     /locus_tag="IWI57_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449553.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            14815..15981
                     /gene="deoB"
                     /locus_tag="IWI57_001402"
     CDS             14815..15981
                     /gene="deoB"
                     /locus_tag="IWI57_001402"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBC2449554.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            16005..16823
                     /locus_tag="IWI57_001403"
     CDS             16005..16823
                     /locus_tag="IWI57_001403"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBC2449555.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            16840..17547
                     /gene="deoD"
                     /locus_tag="IWI57_001404"
     CDS             16840..17547
                     /gene="deoD"
                     /locus_tag="IWI57_001404"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBC2449556.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(17845..18531)
                     /gene="gpmA"
                     /locus_tag="IWI57_001405"
     CDS             complement(17845..18531)
                     /gene="gpmA"
                     /locus_tag="IWI57_001405"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBC2449557.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT         5861 a         3373 c         4152 g         5305 t
ORIGIN      
        1 gttttgaagg ataatgtttt tagaaaagaa cagatagcta gatttcccat ggatttgcta
       61 ttagtatttt gaaaaaatta gtaatttatg acaaaattca ttatactaaa cttgtgcaga
      121 aatgtgaggg atcaactatg aaagcaacaa tgaaagatgt cgcaaaatta gcaggtgttg
      181 gagtaggaac tgtttcccgg gtattgaata acggttctgt taaagcttct actagagaaa
      241 aagttgaagc agcaatgaca gagttaaact atcaaccaga tttttatgcc cgggggttaa
      301 agatgaagca tacgaacacg gttgctttga ttctacccac ggtatggcat ccattttttg
      361 gcgaatttgc ctaccatgta gaaacagcat tagaaagaca aaactacaag ctttatttgt
      421 gtaactccag tggtaatagt gaaaaagaac aagaatatat ccagatggtc agccaaaata
      481 aagtagacgg catcattgga attacttatt cagatatcga tgaatacatt tcgagtaatc
      541 tgccttttgt tagtattgac cgttatttca ctgaagaggt gatttatgtg accgctgaca
      601 atgcggccgg tggacgtttg gctgcaaatg aactttatcg ccgaggatgt cagaatgtag
      661 catatatcgg tgggtaccaa gaaacaccga atgaaacgaa gagtcgtcga aaatattttg
      721 aaatcgagtg ccgtcaactg gggatttcct ataaagcgtt agatatgtta gaacctttag
      781 accatctctc agaacgtgta acaaattttt tgttagagaa tcccgatatc gatgggattt
      841 ttacgatcaa cgatccaatg gcattagctg tcattgatat gcttgaaaaa atagggaaaa
      901 acgtaccaga ggatgtccaa gttatcggat ttgatggtca aaagatgaca gaaggagatc
      961 cttatctagt ctcaacgatt gcccaaccta caaagcaaat ggcggaagtg gcagtggcaa
     1021 aattaattgc tgtcattaat ggagagacaa tagaaaaacg taccgtattg ccgattgaat
     1081 ttggcgaagg tgacacaacg aaacctttag tgagaaacga ttttgcttag tgcaaatacg
     1141 tttctttttt tgaaaatgag tgaatgaaag gtgtagataa aatgttatat ggtggaatag
     1201 aagccggtgg aacaaagttt gtctgtggga ttggtaatga acaattacaa gtgatagaac
     1261 ggaaaagttt tcctaccaca acaccagaag aaacgatgga gcaagtgttt gatttctttg
     1321 ataagtacaa aggaaatatc gcatcaattg gtgtaggagc atttgggcct attgatattc
     1381 aaatcgaatc aagaacttac ggatatatca ccaacacacc gaagttagct tggcaaaatt
     1441 atcattttgt aggagctttg gaaaacgcct tgtcggttcc catctattgg actaccgatg
     1501 taaatgctgc ttgttatgga gaatatatgg cagggtatgg aaaaggtaaa aatagtgttc
     1561 tgtattacac cgttggtaca ggtgttggtg gtggtgctgt agtggatggg aaaatagtcg
     1621 caggttttag tcatcctgaa atgggacata tgctcttaca acgtcataat gaagatcaat
     1681 ttgaaggaat ctgtcctttt catcatgatt gtttggaagg gttagcatca ggacctgcga
     1741 ttgaaaaacg ctttggaaga aaagcccaga ctatctcatt tgatcatcct tattggacga
     1801 ttgaggcaga ttatcttgct cagtgtgtgt ataacacaac gttactatat gcaccggaag
     1861 taataatttt aggcgggggt gtaatgaaac agccgcaact actcgagttg attcgccaat
     1921 cgtttaaaga aaaaatgggt tcctacgtgg atacgcctac tctcgaagaa tatctactaa
     1981 ctccaaggct tgctgacaat gcgggcttga ttggctgcct agctttagca ataaagaaac
     2041 tcaatcatta gaatagtatt tattagaact attcataaca taccggaagt ttaaaagggt
     2101 attctgaaac ttaaatagtc tcaattcatt cttctttctt gagaatctga gtagagactt
     2161 ttattctgtt tcaaattaat ataattacga aatgatattg agtacaagaa agttcttttt
     2221 accagatctt ttaaatcgaa aaatctgata tatatattta tagtactaaa aatctgatta
     2281 gataaaaagc taattcaata ggaaaataaa ggtagtgaat aaaagcaaag cccttgttag
     2341 tagttaatct aacaagggct ttggtaggga aaatggtagg aaaccatctt attttttacg
     2401 aaaatcagga actttagtaa ttgaataatg ttgatttata ggtattcctc atccatatta
     2461 tgttggctct ttattcccac tccactgttg ctggtggttt agaagtgatg tcatacacga
     2521 tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc aagacttccc
     2581 atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca cggataccga
     2641 ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc cctggaagaa
     2701 cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga
     2761 tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca agaacacgaa
     2821 taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc atgcctaact
     2881 gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg atcaattcaa
     2941 attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc tgtgctgttt
     3001 ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa gagacgcctt
     3061 cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca ttcccaatga
     3121 ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag cgatctcttg
     3181 cattgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt acttgttccg
     3241 cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg
     3301 ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga ccaagcaaga
     3361 cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata aagttatcca
     3421 ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc aattcgattc
     3481 cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta cgtgttgtgt
     3541 cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag ccttcaggaa
     3601 ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt gtttctttaa
     3661 acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg cggtttttag
     3721 caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag caaatcccta
     3781 atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc tcatcatata
     3841 cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt acttcttctg
     3901 cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata cgtcttgtga
     3961 tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg ttcgtcatat
     4021 cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca aaatagtcaa
     4081 actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg attttcagtt
     4141 ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg taaatttttc
     4201 aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg atctccgata
     4261 gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa cgcaccaaaa
     4321 cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac agaaaaatca
     4381 aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt gatcccctca
     4441 acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat atcatacaca
     4501 tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg gttcaagaaa
     4561 tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat gattccacgc
     4621 tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt
     4681 cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact ttttccaacg
     4741 gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt atgaagaaat
     4801 aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat cagatgagca
     4861 agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa acttttgata
     4921 ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc tcgccattct
     4981 tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg cgttctccca
     5041 tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga gagattgccg
     5101 atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga cttgcgcacg
     5161 gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa gacatgacaa
     5221 atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg gcttttgagt
     5281 taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt gaaacagtgg
     5341 attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct gatgaaggaa
     5401 agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca tttgcgacac
     5461 agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg caaggaaacg
     5521 caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat gaagcagttc
     5581 atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga aaaaaagttg
     5641 tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca ctgacaattg
     5701 tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa acatttggta
     5761 atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg aaagaataac
     5821 tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta cgggtacgta
     5881 tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga
     5941 aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca gaacttgtat
     6001 agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac tttccttttt
     6061 tctctattct tttcttgggg gttttggtac actgataggg aatttagata gaagggagcc
     6121 aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc agtaacaact
     6181 gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag aaaaaaagtg
     6241 ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa tgtgatcaat
     6301 cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt cttctccaaa
     6361 ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat gttatgcaat
     6421 tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca ccgctcagtg
     6481 gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt agacagcggt
     6541 ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg ggataacgga
     6601 cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat
     6661 ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt agaagaacgt
     6721 ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg gaaatttgtt
     6781 gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt actcaaacat
     6841 aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg
     6901 atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt cgatggatta
     6961 aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag ttttgatatt
     7021 ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag aaaagttccg
     7081 aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt gacaaagggc
     7141 taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg ctttttttat
     7201 ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt ataataccga
     7261 atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa aagagggatg
     7321 gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg aatggaaaga
     7381 ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac atgacaaatg
     7441 aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc gatttgagtt
     7501 ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac acgaccacat
     7561 tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg
     7621 gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga tttcagatcg
     7681 agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg gtaattggtc
     7741 aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga
     7801 ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc gcggcaggag
     7861 cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag aacatcgaag
     7921 atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac cgagaaacag
     7981 tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat agtctagaag
     8041 ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta gaaatgtgct
     8101 acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc gatgaagcaa
     8161 gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga
     8221 tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt ttgactgcac
     8281 gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg
     8341 aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg
     8401 atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca aaaggtgagt
     8461 cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa ttattatata
     8521 aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac atcatcacag
     8581 aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa atggaactaa
     8641 accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct gtgatgcaaa
     8701 ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca gcatgggttg
     8761 cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta atcgggtttc
     8821 ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc atcaataacg
     8881 gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac
     8941 aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag
     9001 ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc gaaatagcaa
     9061 aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc ggagcaaagg
     9121 tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc aaagcttccg
     9181 gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct acaagaatcg
     9241 gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat taattttttc
     9301 aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta ttgaagcgtt
     9361 agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg taacggaatc
     9421 aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa cgaattgtgc
     9481 cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta cgcatctagt
     9541 cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac aagtaatggc
     9601 agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg tgacgaaggc
     9661 aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat aactcatgtt
     9721 cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt tacagaaatg
     9781 aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt atttttaggt
     9841 gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc agacacaaca
     9901 actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa ccagtcagct
     9961 tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa tgatggcttc
    10021 caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca agcattgaat
    10081 gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat ccaagaacaa
    10141 gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga tggattagat
    10201 aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg tgttgcagca
    10261 gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc
    10321 gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga attaggaaaa
    10381 gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa aggacgttca
    10441 attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc aggcggtaca
    10501 ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg
    10561 gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa cggcgaaaag
    10621 aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga agatttggca
    10681 caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg attgaaagaa
    10741 gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc agttgatgaa
    10801 gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga agaaaactaa
    10861 aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg attagaacaa
    10921 gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca actccctgaa
    10981 gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca atcgttgccc
    11041 tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat
    11101 gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca atttacaagt
    11161 aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat ctacattgat
    11221 gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc gtgggaaaga
    11281 agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg tccatcagca
    11341 ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa gcgagcctag
    11401 tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag tttctgaaca
    11461 gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg gaatggaaca
    11521 acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt ttgatgagcc
    11581 gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga aggaattagt
    11641 caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca aagcagtagc
    11701 tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt
    11761 aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca aaacgatgaa
    11821 aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa
    11881 tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg
    11941 tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga ctggtttgcg
    12001 aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata aaaaaccccg
    12061 aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat acgggttagt
    12121 cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa aaccttacag
    12181 taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat tgatcgaaga
    12241 atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag gcggaaatca
    12301 gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa
    12361 tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc tgatcgaaca
    12421 aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt
    12481 ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg atccgaaaga
    12541 aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag aagcacgaaa
    12601 agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc cgtaatttat
    12661 tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg atggctgctt
    12721 ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt ggttcgcaaa
    12781 gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca ttaggttttt
    12841 ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca ctttgcgggt
    12901 ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt gttttgcttc
    12961 cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt ccaggtttac
    13021 ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac tatatcttat
    13081 tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc tcaagtttgg
    13141 actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta acacaagctt
    13201 ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt attttcgttt
    13261 ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga ttgaatcctt
    13321 ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg atggtcattt
    13381 ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct
    13441 ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc ttgcttggtt
    13501 ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg aagatcggtg
    13561 gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt
    13621 tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct aaagtcttcc
    13681 aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg aatcaaaacc
    13741 atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc ttacttctac
    13801 actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt ctgaacgaag
    13861 cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta gttccatcgt
    13921 tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg ctattttagt
    13981 gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag tcagtttgcg
    14041 tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt taggtgtttt
    14101 cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga atctggggta
    14161 tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct ttaaaaatac
    14221 attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga tcatcttcaa
    14281 gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg ccgacacatt
    14341 aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct tgttaggcgg
    14401 tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt
    14461 tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc ctttaggggc
    14521 aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg gcgcacaatt
    14581 accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt tgctgacaat
    14641 cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg ggaagaacta
    14701 tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg
    14761 tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat aatcatgttt
    14821 aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc agatgccgat
    14881 aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc aggattgaac
    14941 gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg tgtggaatca
    15001 gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg
    15061 atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg tgtgtttcct
    15121 aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg taaagtcgta
    15181 tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca ccaaatgaaa
    15241 acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa
    15301 gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga tataacaaaa
    15361 gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga accaggagac
    15421 ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca cacagtcctt
    15481 gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa cgatattttc
    15541 aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg cgttgaccag
    15601 ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt agtagatttt
    15661 gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat tgaagaattt
    15721 gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct aatgattaca
    15781 gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga atatgtaccg
    15841 ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg atattattca
    15901 gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc
    15961 ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc aagttaagcg
    16021 agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa attgattttg
    16081 gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat gctgtaatga
    16141 tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat gcaggaaagc
    16201 tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga ttccactact
    16261 atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc ggactagggg
    16321 cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag
    16381 atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg atcggtccga
    16441 atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg cctgactatc
    16501 aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa ggcgtatata
    16561 tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct cggacaatag
    16621 gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct cacagcggat
    16681 taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa gcgaatctga
    16741 gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa gcattggtta
    16801 aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca tattgaagcg
    16861 aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg agctaaatat
    16921 attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg tatgttaggc
    16981 tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat gggaatgcca
    17041 tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa attgattcga
    17101 gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt actagcacaa
    17161 ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca ttttcctcca
    17221 atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa gggctatacg
    17281 gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt gacagagacg
    17341 ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc tctttattac
    17401 ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga ccatttgatc
    17461 accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat gatcgaagta
    17521 ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct gcattaagta
    17581 ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga cttattattg
    17641 caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt ttaactctga
    17701 gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga
    17761 ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac aaatcgtgtc
    17821 acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg ttaagtcatc
    17881 atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga tgtcttcgtc
    17941 agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg cagctactaa
    18001 aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg gcaaagcgcg
    18061 ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta acattgcgta
    18121 acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag gaagagtatc
    18181 gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg tttctttttt
    18241 gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt tgatttgtgg
    18301 tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg cacgagttaa
    18361 aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc gtccgccttc
    18421 ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag taaattggtt
    18481 caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca tgtttagctc
    18541 tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg tacaattttt
    18601 tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag tagagaatat
    18661 aaaaaaacga acagtgccca cgcaactgtt c
//
LOCUS       DADXTG010000048        18567 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.049, whole genome shotgun sequence.
ACCESSION   DADXTG010000048 DADXTG010000000
VERSION     DADXTG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.049"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            114..1442
                     /locus_tag="IWI57_001406"
     CDS             114..1442
                     /locus_tag="IWI57_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBC2449558.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            1644..2477
                     /locus_tag="IWI57_001407"
     CDS             1644..2477
                     /locus_tag="IWI57_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBC2449559.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2493..3230
                     /locus_tag="IWI57_001408"
     CDS             2493..3230
                     /locus_tag="IWI57_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBC2449560.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3385..4353)
                     /locus_tag="IWI57_001409"
     CDS             complement(3385..4353)
                     /locus_tag="IWI57_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBC2449561.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            4585..5418
                     /locus_tag="IWI57_001410"
     CDS             4585..5418
                     /locus_tag="IWI57_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBC2449562.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5452..6291
                     /locus_tag="IWI57_001411"
     CDS             5452..6291
                     /locus_tag="IWI57_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2449563.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6317..6877
                     /locus_tag="IWI57_001412"
     CDS             6317..6877
                     /locus_tag="IWI57_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBC2449564.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7076..8797
                     /locus_tag="IWI57_001413"
     CDS             7076..8797
                     /locus_tag="IWI57_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBC2449565.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            8962..10104
                     /locus_tag="IWI57_001414"
     CDS             8962..10104
                     /locus_tag="IWI57_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBC2449566.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10120..11331
                     /gene="thiI"
                     /locus_tag="IWI57_001415"
     CDS             10120..11331
                     /gene="thiI"
                     /locus_tag="IWI57_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBC2449567.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            11904..12551
                     /locus_tag="IWI57_001416"
     CDS             11904..12551
                     /locus_tag="IWI57_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBC2449568.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    12680..12896
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12944..15589
                     /locus_tag="IWI57_001417"
     CDS             12944..15589
                     /locus_tag="IWI57_001417"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBC2449569.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            15899..17212
                     /locus_tag="IWI57_001418"
     CDS             15899..17212
                     /locus_tag="IWI57_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBC2449570.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17202..17855
                     /locus_tag="IWI57_001419"
     CDS             17202..17855
                     /locus_tag="IWI57_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2449571.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            17910..>18567
                     /gene="radC"
                     /locus_tag="IWI57_001420"
     CDS             17910..>18567
                     /gene="radC"
                     /locus_tag="IWI57_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBC2449572.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     S"
BASE COUNT         6262 a         3121 c         3956 g         5228 t
ORIGIN      
        1 gaagttgtaa gtatattctc attcaaatga aaatttcatc atagaagaga ttgtatttct
       61 aaattcttaa gagtaaaata cgaagtataa cttggatttg gaaggatgat tggatgaaag
      121 ctattacgcc tgatatgaca acaaaattta acaaacagtt tcaagcaaat atcaaacagc
      181 aggcattgca gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa aatgtttcag
      241 cacatgtgga gaatacaccg gtattttcta tagatttggc tacggggaaa gtggctaatc
      301 aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa cacattccgt cataagatgc
      361 tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa ttacactttt ttctgggata
      421 aatatgaaaa ggcaaattat ttctatgaaa acatcattgc aacaggaaaa gaggcaatcg
      481 atagccggaa agtagctttc ttactcgcta ctcctcaaca agatggcggc caatgggata
      541 tgatcgtttc attattccaa aaatatggcg ttgtgcctaa atcggtgatg ccggaaagtg
      601 cgaatagttc aaattctcga gatttgaata actacttgaa caaattactg cgtaaagatg
      661 cagtagtgtt acggaaaatg gttgctcaag gagaaacatt agacgctatt gaagaaaaga
      721 aagaagaaat gctagaaagc atttataact tcttagctat ctcattagga acacctccaa
      781 aagaattcga ttttgagtac cgcgatgaag aaaagaacta tcatcttgac cgaggactga
      841 caccacaaat tttttatgat aaatacatcg gtgtaaaatt agatgattat gttagcgtga
      901 tcaatgcacc aacaaaagac aaaccattca atcgttctta tacagtagag atgctaggaa
      961 atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga tatggaaact ttcaaaaaat
     1021 tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat gtaggtcaat
     1081 cttctactag aactagtggc atcatggcct tagatgctta ttcaatggat gatctatttg
     1141 atacggattt tacaatgact aaagctgaac gtttagattt tggagaaagt ctgatgaccc
     1201 atgcgatggt attgaccggt gttgacttag tagacgggca atcaacaaaa tggaaagtcg
     1261 aaaatagctg gggcgaaaaa gttggaaaga atggcttctt tgttatgagt gatgcctgga
     1321 tggacgaata tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa gagcaattaa
     1381 atgcgttcaa tgaagaacca attgttctat ctccgtggga tccaatgggt gctttagcat
     1441 agacgagtat ttcaatgaag agtatgtagt aaagataaaa agagctggct taagccagct
     1501 ctttttatct ttaaagtcca ttccctgttt tttatggtcc ctgtgctata attctaaata
     1561 aaggataagg attggaaata atccttttat cattaaaaat atgatgacga agtagaataa
     1621 aattgggagt aggggggaaa gtcatggata tccaaactgt tttatctgat ctgaaattaa
     1681 atggtaaagt gattccagtt gttggtggcg atgttaatca gacctatcgt ataaaaaccg
     1741 aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa aaaaggattt tttgaagcag
     1801 aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt accggatacg tatatgctag
     1861 gtgaaacaag tgaaggcgct tatttgttaa tggaatggat cgagccggga aaaggggatc
     1921 aacgagatct tgctgcagct ttagctaatc tccatcagca aacagcgcca caatttggtt
     1981 ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa ttcttttgaa gaagattggt
     2041 ggactttttt ctttaaagat cggcttgaat cccagatttc tttggcagaa gaaacgaatc
     2101 gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta ttgaaatcgg
     2161 ttgaaccaaa aaagatcact ccacgcctct tgcacggaga cctgtggagt ggtaatgtgt
     2221 tctttgacca gcaagggcat ccagtttttg tcgacccagc tgtttcttat gggaatcggg
     2281 aacaagatat tgcaatgagc cagttattcg gtggtttccg accggaattt ttagatgctt
     2341 atcaaactat atttccatta gaaaagggat ggaaagaccg actgccgatt taccagttat
     2401 attatctttt ggcacatttg aatatgtttg gagaaagtta cggttcccaa gtagatcaat
     2461 tactagaaaa tttttaagag gagggcgtgt ttttggcaaa aaaatatacc aaaaagcatg
     2521 aagtttcata ttatgagtgt gacatcaacc aaacgatgac atttccttcg atgttaggag
     2581 tagttatcaa aacgtctgaa gatcaaagcg accagttggg aagaggcagt gaattcgtca
     2641 atagctttgg acttacttgg gtcatcacga attattccat ggagattaat cgtctcccta
     2701 gagtcggtga agtaatctca gtaacgactc aagccatgga gtacaataag tttttctgtt
     2761 atcggaactt ctggatccat gatgaagatg ggaaagaatt agtcaaaata gaatctgttt
     2821 tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag atcattgctc
     2881 cttttgagag tgagaagatc aagaaaatca agcgtcagga aaaaattgaa aagattgatt
     2941 cgggcttcat gttgccatat cgtgttcgtt tttatgatat cgatagtaat cagcatgtta
     3001 ataatgcgat gtattttaat tggatcattg acgtattagg atatgacttt ttgactacgc
     3061 atgttccaga aaaagtaatt attcgatttg ataaagaagt agagtacggg aatgagatcg
     3121 aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat gaaatccgaa
     3181 tgcaagaaca gctgtattgt gaagcaaata ttatatggaa aaataaataa aaaagaggtt
     3241 gtgacagaag ggggcaactt caagaaataa gaagaaattt acgaaaattg tttctcaaat
     3301 ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc tctcttttta
     3361 agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt cttcacccga
     3421 caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat taatactgtt
     3481 acagaaaata atccccattt tttttagttc ttttcccccg ccttcataat cataaacagg
     3541 ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt ggacaagaag
     3601 cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag cttcgattac
     3661 caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca tcccagcata
     3721 tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct ttgtttctaa
     3781 aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac ccaatgtagg
     3841 tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg cagaatggat
     3901 ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac ttgcgacacg
     3961 aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg aacgcattgc
     4021 cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa aagcagtttc
     4081 ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg cataagcgga
     4141 caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg agggaagatt
     4201 gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg catctaataa
     4261 tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca tggcgatcgt
     4321 tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac gaataatttt
     4381 gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca gtgaagaata
     4441 acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa gactcaacct
     4501 tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag cagccaagga
     4561 aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt taaagaacat
     4621 gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt tctaccctta
     4681 gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga gaattggata
     4741 agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat cagcggtcaa
     4801 ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt tttagatgtt
     4861 atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt cccctcatca
     4921 ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg gtttgagggt
     4981 tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt tcttgaacgt
     5041 acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga gccacagttg
     5101 ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc tctttttcga
     5161 ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg taaaaaaaga
     5221 acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt caaagtagtt
     5281 gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg tgatgcggct
     5341 gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact caatccaacc
     5401 caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc tatgatcaaa
     5461 gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc gttggaatca
     5521 acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt agcaacgggg
     5581 cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat gtatgtcgtg
     5641 tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc ttttgaacaa
     5701 aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat cgtttttggt
     5761 gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat ttttatcaag
     5821 cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa aatcctccag
     5881 gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt aaaagaacca
     5941 atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga acatcgcttt
     6001 cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc aaaaggtggt
     6061 tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat ggaagaaatt
     6121 atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg aattggagtg
     6181 gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac tcaatcaaat
     6241 gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta gtcaatgaaa
     6301 gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa cattccacaa
     6361 aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg aagctcatac
     6421 acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt cacctaataa
     6481 agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag cagaagaaaa
     6541 aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag atgcactgac
     6601 agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc ctgatcgaat
     6661 attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa aacctcatta
     6721 tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat atgctccaga
     6781 acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta agaagaaatc
     6841 cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact atttttgtta
     6901 tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca tgaaaatagt
     6961 atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg atttatgcca
     7021 agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac cataaatggg
     7081 aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg tcgtcttttt
     7141 ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag aaaaacgcaa
     7201 ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa aaatgcatct
     7261 ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg acatatctac
     7321 ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca atgaaagttt
     7381 tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg accaaatgga
     7441 gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg aagaacgcaa
     7501 ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa aagtcagaga
     7561 agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca aagtgatcga
     7621 aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg aagcccgcga
     7681 ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga aaaaaattcc
     7741 accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa tcagtgatac
     7801 ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg ctgaagatat
     7861 cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa cagaagttga
     7921 aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat atgatatctt
     7981 agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga ttgcagagta
     8041 tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc acacatctca
     8101 aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa ccgaagtgga
     8161 agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg aaattcctta
     8221 ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata tcgaaaagca
     8281 acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag aagcgcaaga
     8341 aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag aaaaacaacg
     8401 tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg accgtattga
     8461 agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca atcgtttagt
     8521 tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt tagtagatgc
     8581 agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata cacacgaaga
     8641 agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc actatcaaga
     8701 agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt tcaaacgcat
     8761 cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta gtaaaccagc
     8821 aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga aaattgaaac
     8881 aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat agaaaagaat
     8941 ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac cccaatcttt
     9001 ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa tccatcaagc
     9061 ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa gcagatcgct
     9121 gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac agaaggagat
     9181 aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca tatgatcatt
     9241 tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga attaggaatc
     9301 gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga attgaaagaa
     9361 ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga agtaggatcg
     9421 attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat tcatttccat
     9481 gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac agaaagagtt
     9541 gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg tttcatttat
     9601 tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga aagaaatcag
     9661 cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt acgtttatca
     9721 ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc tctaatcaat
     9781 gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta tgcaccgcat
     9841 attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc cttagaagaa
     9901 aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat ggcaagcagt
     9961 acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg aatcagctta
    10021 gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa gcagctttat
    10081 aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag tgaaattatg
    10141 gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat catgcaacta
    10201 gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca tgctgatcgg
    10261 gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc aaaactagca
    10321 aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga tgtcgaagtt
    10381 ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga aacatttaaa
    10441 attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt gaatcaaaca
    10501 ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg tcaaccagat
    10561 attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga aacgatcaag
    10621 ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct ttctgggggg
    10681 attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat tgaagctgtt
    10741 cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa agatctaaca
    10801 gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt taccgagatt
    10861 caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac tcgtcgaatg
    10921 atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat tttgaatggt
    10981 gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat caatgaagta
    11041 acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat cattgaagta
    11101 gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg ctgtacgatt
    11161 tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca atatgaagag
    11221 cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat ggaagagata
    11281 caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata agaggttatg
    11341 aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct ccatgtattt
    11401 taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc aagaagttat
    11461 ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag atcaagctac
    11521 agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg tgacagaagt
    11581 gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt tttgtgaaat
    11641 ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg ttgcgaaaaa
    11701 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat attttacgtt
    11761 tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg tctcacaccc
    11821 ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact aaatatgtga
    11881 aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca aaagcaactg
    11941 cgaaaagatt acctttatat ttacgctact tgaagatgct ggatgactca ggtataagta
    12001 gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg atccgcagag
    12061 acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc ccttttctta
    12121 tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca ttagtaggat
    12181 atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa aatctaaata
    12241 tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat ggagaaatga
    12301 tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca gtagcaatct
    12361 ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa ggaaatgtaa
    12421 ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc aatgttcaat
    12481 acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat tactcagaag
    12541 ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt cgtaattctt
    12601 tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt gactaaaaaa
    12661 aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc acattcattc
    12721 ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg tagcttggga
    12781 gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca cttgagaggc
    12841 aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc ctagaatacg
    12901 aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg aagaaaataa
    12961 cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc attggttaga
    13021 acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa tcgtaatccc
    13081 gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga ccttgcaaga
    13141 tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc ctggtatgga
    13201 ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc aagggatttc
    13261 tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga aagaagagta
    13321 tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt acagtaggga
    13381 acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg ttaccttata
    13441 cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc aagcacgaac
    13501 agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt atcatatgag
    13561 ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc cagaaacgat
    13621 gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag cgctcatcgg
    13681 caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag atgaatatgt
    13741 agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc caaacgactt
    13801 tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg atggtacgat
    13861 gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta aagcaatcgt
    13921 gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc acagtgtggg
    13981 tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat ggtttgtaaa
    14041 aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag cagtggaatt
    14101 cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc atgattgggt
    14161 aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca aagaaacagg
    14221 tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac aagatcctga
    14281 tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg gctggccaga
    14341 tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa ccggttatga
    14401 catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta ctggcgaacg
    14461 tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc ggaagatgag
    14521 taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg gagcagatgc
    14581 tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct tcagctatga
    14641 aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac gtttcgttat
    14701 catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa aatcagtggc
    14761 tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg agctatttga
    14821 tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg atgatttttg
    14881 tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg tttcaaaaca
    14941 aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat tgcatccaat
    15001 catgccattc gttacagaag aaatatggga acatattcct catcagggaa attctctagt
    15061 agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt ctaaagggat
    15121 ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag tgaatacacc
    15181 attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg atcaattttt
    15241 agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga cgatatcaag
    15301 tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag aaatcttctt
    15361 gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa aagaactgga
    15421 aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac gttttgtttc
    15481 taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt atttagagaa
    15541 gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag aatagaaaaa
    15601 gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc gttacaggaa
    15661 agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa attttgtcgc
    15721 atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat catgtatcaa
    15781 gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc tttgtcatac
    15841 ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg aattgttgat
    15901 gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt cacgtcctgg
    15961 ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa aagtaaaaac
    16021 gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga gaagcctatt
    16081 agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa cgttcaatga
    16141 acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc ttgtaaaaaa
    16201 atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta ttactgaatt
    16261 cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg acgtagcgat
    16321 tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac caatgctgac
    16381 gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa ttgaagaaat
    16441 cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag gaaatattgc
    16501 cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac cgctttatgt
    16561 atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg gagaagtatt
    16621 tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt tagggcaaca
    16681 tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc aattggaaaa
    16741 gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat ggccaggaag
    16801 aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata atacgcatgc
    16861 aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa tcaacatttt
    16921 gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc agcaagtacc
    16981 aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat taagtgaagt
    17041 atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg gattagcaga
    17101 tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac tttatttcat
    17161 ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat aatgcagtga
    17221 tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa gccacacaag
    17281 tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa ttcttaggtg
    17341 tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt ggcaaaacaa
    17401 gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt tcgcaaggag
    17461 cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa catcagattc
    17521 ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg aagaagaaca
    17581 atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga gcaaaaccag
    17641 acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa gaaacattga
    17701 ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc ccggtagtca
    17761 tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct ggggttgttt
    17821 cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt cgtatcttat
    17881 tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa gaagtaccta
    17941 ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta tcggatcagg
    18001 agcttttggc aatcttactg cgtaccggtc aacaccccta tagtgtgatg tcgattgctg
    18061 gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact ttgcatgagt
    18121 tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc ttgatcgaat
    18181 taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt accagctacg
    18241 aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat ttggtttgta
    18301 tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc ggttcgctga
    18361 atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat tgtgcagcaa
    18421 ggatcgtttt agcgcataat catccttccg gaatatataa gttagtacac ataaattata
    18481 taaggaaatt tactgttata ttgccatctg gaaatactat tatgttatcg ataattgaac
    18541 gccataatgc atttttttct tcttccg
//
LOCUS       DADXTG010000049        18194 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.050, whole genome shotgun sequence.
ACCESSION   DADXTG010000049 DADXTG010000000
VERSION     DADXTG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.050"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(105..560)
                     /locus_tag="IWI57_001421"
     CDS             complement(105..560)
                     /locus_tag="IWI57_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449573.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IWI57_001422"
     CDS             complement(714..1922)
                     /locus_tag="IWI57_001422"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBC2449574.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IWI57_001423"
     CDS             complement(2034..2366)
                     /locus_tag="IWI57_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBC2449575.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IWI57_001424"
     CDS             2514..3389
                     /locus_tag="IWI57_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2449576.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IWI57_001425"
     CDS             complement(3471..4052)
                     /locus_tag="IWI57_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBC2449577.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IWI57_001426"
     CDS             complement(4058..5317)
                     /locus_tag="IWI57_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBC2449578.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IWI57_001427"
     CDS             complement(5319..6365)
                     /locus_tag="IWI57_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449579.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="IWI57_001428"
     CDS             complement(6600..6875)
                     /locus_tag="IWI57_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBC2449580.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /gene="der"
                     /locus_tag="IWI57_001429"
     CDS             complement(7118..8428)
                     /gene="der"
                     /locus_tag="IWI57_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBC2449581.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IWI57_001430"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IWI57_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBC2449582.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9974..10654)
                     /locus_tag="IWI57_001431"
     CDS             complement(9974..10654)
                     /locus_tag="IWI57_001431"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBC2449583.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10703..11338)
                     /locus_tag="IWI57_001432"
     CDS             complement(10703..11338)
                     /locus_tag="IWI57_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2449584.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11404..12807)
                     /locus_tag="IWI57_001433"
     CDS             complement(11404..12807)
                     /locus_tag="IWI57_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBC2449585.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12794..13825)
                     /locus_tag="IWI57_001434"
     CDS             complement(12794..13825)
                     /locus_tag="IWI57_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449586.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13856..14077
                     /locus_tag="IWI57_001435"
     CDS             13856..14077
                     /locus_tag="IWI57_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBC2449587.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14242..14979)
                     /locus_tag="IWI57_001436"
     CDS             complement(14242..14979)
                     /locus_tag="IWI57_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBC2449588.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15132..16472)
                     /locus_tag="IWI57_001437"
     CDS             complement(15132..16472)
                     /locus_tag="IWI57_001437"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBC2449589.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16709..>18194)
                     /locus_tag="IWI57_001438"
     CDS             complement(16709..>18194)
                     /locus_tag="IWI57_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBC2449590.1"
                     /translation="AYTVLFIIIAVIAVLTWFIPAGHYSVDDAGNIVAGSYERVQQNP
                     QGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGIINKTGALDAGIAS
                     VVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMMAVGFDTVVAVAIV
                     LVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLAISIVYVYRYASKI
                     EKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFITFGIMIISLIPWTT
                     LNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFLVMSIVVAITYGMK
                     ESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWGEQGLSSLSSSVFI
                     IITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITAYQSASGLVNLITP
                     TSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5130 a         3885 c         3086 g         6093 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     9901 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     9961 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    10021 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    10081 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    10141 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    10201 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    10261 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    10321 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    10381 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    10441 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    10501 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    10561 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    10621 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    10681 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    10741 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    10801 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    10861 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    10921 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    10981 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    11041 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    11101 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    11161 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    11221 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    11281 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    11341 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    11401 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    11461 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    11521 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    11581 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    11641 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    11701 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    11761 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    11821 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    11881 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    11941 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    12001 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    12061 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    12121 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    12181 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    12241 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    12301 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    12361 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    12421 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    12481 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    12541 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    12601 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    12661 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    12721 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    12781 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    12841 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    12901 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    12961 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    13021 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    13081 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    13141 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    13201 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    13261 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    13321 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
    13381 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    13441 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    13501 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    13561 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    13621 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    13681 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    13741 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    13801 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    13861 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    13921 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    13981 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    14041 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    14101 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    14161 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    14221 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    14281 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    14341 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    14401 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    14461 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    14521 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    14581 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    14641 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    14701 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    14761 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    14821 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    14881 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    14941 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    15001 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    15061 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    15121 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    15181 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    15241 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    15301 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    15361 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    15421 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    15481 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    15541 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    15601 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    15661 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    15721 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    15781 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    15841 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    15901 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    15961 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    16021 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    16081 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    16141 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    16201 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    16261 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    16321 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    16381 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    16441 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    16501 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    16561 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    16621 agctattgac ttgtccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    16681 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    16741 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    16801 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    16861 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    16921 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    16981 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    17041 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    17101 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    17161 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    17221 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    17281 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    17341 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    17401 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    17461 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    17521 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    17581 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    17641 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    17701 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    17761 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    17821 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    17881 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    17941 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    18001 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    18061 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    18121 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    18181 agaccgtata cgct
//
LOCUS       DADXTG010000050        18077 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.051, whole genome shotgun sequence.
ACCESSION   DADXTG010000050 DADXTG010000000
VERSION     DADXTG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.051"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(94..164)
                     /locus_tag="IWI57_001439"
     tRNA            complement(94..164)
                     /locus_tag="IWI57_001439"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Gly,seq:ccc)
     gene            281..1033
                     /locus_tag="IWI57_001440"
     CDS             281..1033
                     /locus_tag="IWI57_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBC2449591.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            1049..1528
                     /locus_tag="IWI57_001441"
     CDS             1049..1528
                     /locus_tag="IWI57_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBC2449592.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(1779..3359)
                     /locus_tag="IWI57_001442"
     CDS             complement(1779..3359)
                     /locus_tag="IWI57_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBC2449593.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3352..4095)
                     /locus_tag="IWI57_001443"
     CDS             complement(3352..4095)
                     /locus_tag="IWI57_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449594.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(4271..5047)
                     /locus_tag="IWI57_001444"
     CDS             complement(4271..5047)
                     /locus_tag="IWI57_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2449595.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(5295..6131)
                     /locus_tag="IWI57_001445"
     CDS             complement(5295..6131)
                     /locus_tag="IWI57_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBC2449596.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(6142..7071)
                     /locus_tag="IWI57_001446"
     CDS             complement(6142..7071)
                     /locus_tag="IWI57_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBC2449597.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            7263..7616
                     /locus_tag="IWI57_001447"
     CDS             7263..7616
                     /locus_tag="IWI57_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBC2449598.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(7690..8346)
                     /locus_tag="IWI57_001448"
     CDS             complement(7690..8346)
                     /locus_tag="IWI57_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBC2449599.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            8572..10506
                     /locus_tag="IWI57_001449"
     CDS             8572..10506
                     /locus_tag="IWI57_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2449600.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(10592..11815)
                     /locus_tag="IWI57_001450"
     CDS             complement(10592..11815)
                     /locus_tag="IWI57_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBC2449601.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(11809..13707)
                     /locus_tag="IWI57_001451"
     CDS             complement(11809..13707)
                     /locus_tag="IWI57_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBC2449602.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(13701..14819)
                     /locus_tag="IWI57_001452"
     CDS             complement(13701..14819)
                     /locus_tag="IWI57_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBC2449603.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(14902..15735)
                     /locus_tag="IWI57_001453"
     CDS             complement(14902..15735)
                     /locus_tag="IWI57_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2449604.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(15728..16645)
                     /locus_tag="IWI57_001454"
     CDS             complement(15728..16645)
                     /locus_tag="IWI57_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2449605.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(16668..16847)
                     /locus_tag="IWI57_001455"
     CDS             complement(16668..16847)
                     /locus_tag="IWI57_001455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449606.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(16872..17996)
                     /locus_tag="IWI57_001456"
     CDS             complement(16872..17996)
                     /locus_tag="IWI57_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBC2449607.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5667 a         3560 c         3027 g         5823 t
ORIGIN      
        1 taataagtcc aaccaagtca aaaacataag aagctttttt gcttgactat tcttattact
       61 tgatgcaggg cagctttttc acagcctctt ttaagcgggt gacgggaatc gaacccgcga
      121 catcagcttg ggaagctggg attttaccac taaactacac ccgcgataca aaaacagtat
      181 acgcctcttt cactccttcc tcaagttttt ttattcttta cgcttccgat atgttttgta
      241 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
      301 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
      361 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
      421 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
      481 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
      541 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
      601 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
      661 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
      721 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
      781 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
      841 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
      901 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
      961 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
     1021 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
     1081 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
     1141 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
     1201 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
     1261 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
     1321 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
     1381 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
     1441 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
     1501 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
     1561 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
     1621 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
     1681 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
     1741 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
     1801 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
     1861 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
     1921 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
     1981 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
     2041 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
     2101 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
     2161 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
     2221 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
     2281 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
     2341 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
     2401 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
     2461 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
     2521 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
     2581 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
     2641 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
     2701 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
     2761 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
     2821 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
     2881 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
     2941 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
     3001 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
     3061 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
     3121 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
     3181 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
     3241 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
     3301 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
     3361 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
     3421 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
     3481 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
     3541 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
     3601 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
     3661 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
     3721 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
     3781 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
     3841 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
     3901 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
     3961 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
     4021 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
     4081 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
     4141 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
     4201 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
     4261 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
     4321 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
     4381 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
     4441 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
     4501 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
     4561 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
     4621 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
     4681 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
     4741 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
     4801 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
     4861 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
     4921 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
     4981 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
     5041 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
     5101 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
     5161 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
     5221 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
     5281 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
     5341 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
     5401 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
     5461 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
     5521 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
     5581 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
     5641 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
     5701 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
     5761 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
     5821 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
     5881 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
     5941 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
     6001 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
     6061 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
     6121 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
     6181 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
     6241 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
     6301 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
     6361 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
     6421 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
     6481 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
     6541 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
     6601 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
     6661 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
     6721 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
     6781 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
     6841 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
     6901 agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg
     6961 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
     7021 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
     7081 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
     7141 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
     7201 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
     7261 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
     7321 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
     7381 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
     7441 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
     7501 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
     7561 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
     7621 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
     7681 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
     7741 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
     7801 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
     7861 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
     7921 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
     7981 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
     8041 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
     8101 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
     8161 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
     8221 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
     8281 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
     8341 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
     8401 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
     8461 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
     8521 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
     8581 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
     8641 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
     8701 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
     8761 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
     8821 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
     8881 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
     8941 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
     9001 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
     9061 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
     9121 gacattttga ttctagacga accaacgaac catttagata tcgagacact ttcctggctt
     9181 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
     9241 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
     9301 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
     9361 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
     9421 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
     9481 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
     9541 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
     9601 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
     9661 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
     9721 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
     9781 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
     9841 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
     9901 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
     9961 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    10021 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    10081 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    10141 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    10201 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    10261 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    10321 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    10381 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    10441 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    10501 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    10561 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    10621 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    10681 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    10741 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    10801 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    10861 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    10921 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    10981 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    11041 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    11101 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    11161 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    11221 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    11281 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    11341 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    11401 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    11461 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    11521 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    11581 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    11641 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    11701 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    11761 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    11821 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    11881 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    11941 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    12001 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    12061 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    12121 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    12181 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    12241 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    12301 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    12361 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    12421 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    12481 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    12541 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    12601 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    12661 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    12721 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    12781 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    12841 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    12901 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    12961 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    13021 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    13081 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    13141 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    13201 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    13261 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    13321 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    13381 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    13441 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    13501 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    13561 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    13621 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    13681 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    13741 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    13801 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    13861 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    13921 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    13981 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    14041 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    14101 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    14161 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    14221 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    14281 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    14341 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    14401 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    14461 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    14521 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    14581 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    14641 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    14701 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    14761 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    14821 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    14881 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    14941 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    15001 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    15061 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    15121 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    15181 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    15241 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    15301 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    15361 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    15421 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    15481 agctgaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    15541 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    15601 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    15661 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    15721 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    15781 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    15841 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    15901 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    15961 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    16021 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    16081 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    16141 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    16201 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    16261 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    16321 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    16381 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    16441 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    16501 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    16561 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    16621 tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt
    16681 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    16741 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    16801 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    16861 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    16921 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    16981 aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt
    17041 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    17101 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    17161 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    17221 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    17281 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    17341 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    17401 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    17461 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    17521 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    17581 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    17641 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    17701 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    17761 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    17821 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    17881 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    17941 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    18001 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    18061 ttagtaacac aaatatt
//
LOCUS       DADXTG010000051        17399 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.052, whole genome shotgun sequence.
ACCESSION   DADXTG010000051 DADXTG010000000
VERSION     DADXTG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.052"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(139..684)
                     /locus_tag="IWI57_001457"
     CDS             complement(139..684)
                     /locus_tag="IWI57_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2449608.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(696..1388)
                     /locus_tag="IWI57_001458"
     CDS             complement(696..1388)
                     /locus_tag="IWI57_001458"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBC2449609.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1407..1691)
                     /locus_tag="IWI57_001459"
     CDS             complement(1407..1691)
                     /locus_tag="IWI57_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449610.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1704..2267)
                     /locus_tag="IWI57_001460"
     CDS             complement(1704..2267)
                     /locus_tag="IWI57_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBC2449611.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2495..3157
                     /locus_tag="IWI57_001461"
     CDS             2495..3157
                     /locus_tag="IWI57_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBC2449612.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3281..4477
                     /locus_tag="IWI57_001462"
     CDS             3281..4477
                     /locus_tag="IWI57_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBC2449613.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4578..5954)
                     /locus_tag="IWI57_001463"
     CDS             complement(4578..5954)
                     /locus_tag="IWI57_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2449614.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5991..6857)
                     /locus_tag="IWI57_001464"
     CDS             complement(5991..6857)
                     /locus_tag="IWI57_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBC2449615.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6946..7971)
                     /locus_tag="IWI57_001465"
     CDS             complement(6946..7971)
                     /locus_tag="IWI57_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBC2449616.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7989..8663)
                     /locus_tag="IWI57_001466"
     CDS             complement(7989..8663)
                     /locus_tag="IWI57_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2449617.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8847..9740)
                     /locus_tag="IWI57_001467"
     CDS             complement(8847..9740)
                     /locus_tag="IWI57_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2449618.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9814..11511)
                     /locus_tag="IWI57_001468"
     CDS             complement(9814..11511)
                     /locus_tag="IWI57_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBC2449619.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11504..12817)
                     /locus_tag="IWI57_001469"
     CDS             complement(11504..12817)
                     /locus_tag="IWI57_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBC2449620.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12817..17325)
                     /locus_tag="IWI57_001470"
     CDS             complement(12817..17325)
                     /locus_tag="IWI57_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBC2449621.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEGELPLQVVV
                     DERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSGQALQRIQRQLFNGILER
                     LGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDG
                     ENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYN
                     GAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASM
                     SELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSD
                     IFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVV
                     GDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFT
                     HPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAI
                     NTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTP
                     RYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPG
                     ENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQV
                     KLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQ
                     NGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWI
                     REIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYY
                     EGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFEL
                     YETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPD
                     SFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENW
                     QYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLY
                     RAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNG
                     YRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLEL
                     LKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKE
                     VKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQ
                     QELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVR
                     QSKKEGAR"
BASE COUNT         4885 a         3902 c         3022 g         5590 t
ORIGIN      
        1 aataaagaga tttattaacg taaaaagaat aagttttttt aaaataacgt ataaaaaaga
       61 gcataaaaca aagtcgtttg ttttatgctc ttcttttatt attttgtgct ttttatttta
      121 tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa aatgtctcaa
      181 tgcccattca tgtccaacgg gatcgaaact tgccacggca tctttgaaaa tatggagttt
      241 gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg tacatacgcc
      301 ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat ctgttccact
      361 aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct catagtcttg
      421 gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca cattatgcgg
      481 aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat caatggcaaa
      541 aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct ttttttcaat
      601 tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat catatgtgta
      661 gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt gacaaattcg
      721 atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa gctttgtgtc
      781 gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat attgaactga
      841 cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc agggaaattc
      901 gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt aacgatcaat
      961 ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact gcgcaaatat
     1021 gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta gccaaatcct
     1081 gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc tacttgtaag
     1141 ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc gtattgttgg
     1201 atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg tacgacgatc
     1261 acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc ccatgataat
     1321 ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc gataataccg
     1381 attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa aaccacacat
     1441 aacattagga taacaataat aagagatttc atcaaaaaag agttccattt ggtcgtcttt
     1501 tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc tttacgataa
     1561 aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg ctgttttttt
     1621 aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt aataagcggt
     1681 cctttggcca taagccgatc cctctacttt cctttggtca atagttccca gtattgaatc
     1741 gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg cgcctcttca
     1801 agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg attctcgacc
     1861 ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc tggtgaaaga
     1921 tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc ttctaattct
     1981 ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc agctggaatc
     2041 tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag acgatcttgt
     2101 tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct cttcgaagtt
     2161 cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc gctatagatt
     2221 tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc ttctcctgct
     2281 ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa actccttatt
     2341 agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc tttagtccga
     2401 tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg aaaagaattc
     2461 aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt ggccgtggct
     2521 tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt ttgttctagg
     2581 acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc gcaggaaaaa
     2641 gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact atgaaaaaag
     2701 cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa caaaaaatga
     2761 gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa tcgatctccg
     2821 ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag aaccaaaccg
     2881 actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg atttgacaaa
     2941 taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg aaaaactaga
     3001 agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt atcaaaaatt
     3061 cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac agagcttaaa
     3121 gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat aaaagaagac
     3181 tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta gagacattca
     3241 tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata aaccgactga
     3301 aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca acccgttttc
     3361 aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc tccaagagca
     3421 acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac aagctaaaca
     3481 gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg gatcggctgc
     3541 ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag cacaagatat
     3601 tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag ccaatcctga
     3661 cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg tcaaacaatc
     3721 gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca agatttcagt
     3781 gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag aacagcttta
     3841 ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg gagaattgaa
     3901 aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag aagaaactgg
     3961 cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc gtctggataa
     4021 acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac cacaaatccg
     4081 tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta tcgctacagc
     4141 cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc aaaaagatgc
     4201 agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga aaaattcaga
     4261 aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa tcgtggatat
     4321 cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga cattgcgtat
     4381 ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac acatggaaga
     4441 agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct tgttactaaa
     4501 acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta tctttcttct
     4561 aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc atagccaaaa
     4621 taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac agtttgcaga
     4681 cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg aacgaatggc
     4741 actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag taagcctaga
     4801 ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa tacattcaat
     4861 gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc ttttttcggt
     4921 gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc cttgctgaca
     4981 gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag tagcgttaaa
     5041 attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct cattgcaaaa
     5101 gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa gatcagcgtc
     5161 acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct tggcacatct
     5221 ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc caagaaagca
     5281 aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat gaatggctgc
     5341 cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat gataccaaat
     5401 ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag cgacacgatg
     5461 taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc attttccgga
     5521 ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa aaaaccttgc
     5581 atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc cccgatcaac
     5641 aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata ggcatatggc
     5701 ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga caaaatgaca
     5761 acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg attggccgca
     5821 acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag catgatcaaa
     5881 tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat ctctttctta
     5941 cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga ttaaactgtt
     6001 ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac aataacataa
     6061 atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa agtagagata
     6121 atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt gactgttaga
     6181 agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt ccacacaaac
     6241 tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat tccgatactt
     6301 tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat ggtatataca
     6361 ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc aagcatttta
     6421 ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc accgcttaca
     6481 agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca ttcatggaag
     6541 aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa tggtagccct
     6601 acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac actccaagct
     6661 aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact aacaacgaaa
     6721 aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg ttgattggca
     6781 tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat agggactaat
     6841 gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa cttatcagcg
     6901 aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac gtttttcatt
     6961 ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt cagttgattc
     7021 agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa gtcctaatcc
     7081 tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat caaagatccg
     7141 tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc tgactccttg
     7201 cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt tgatggcatt
     7261 actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa cagtatgatc
     7321 ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct gactgcgtat
     7381 cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc gcgagaaact
     7441 gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg ataattcatt
     7501 ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa ctagcaattt
     7561 agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat ctttttggtc
     7621 ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca gctgattgat
     7681 atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat tttgcaaagg
     7741 ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca agtaaccacc
     7801 cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa ttgtgctcaa
     7861 atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa taaaaaacag
     7921 ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca tcttaaccgc
     7981 tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata tccttggata
     8041 cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt attgtcatct
     8101 acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa gatctttcca
     8161 ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc aatgatctct
     8221 ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg gctcatcatt
     8281 tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat ttttgcaatc
     8341 agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt catagccatg
     8401 atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt cgatacttca
     8461 cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc tagtaataca
     8521 agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt cgttccaaag
     8581 gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat ctcttcgtct
     8641 tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc tactacaata
     8701 tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt agcaaacgtt
     8761 aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag cttaaattta
     8821 ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg tttttgtctt
     8881 ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat acctagaagg
     8941 agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga agagacttct
     9001 tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac ggtgtaaggt
     9061 gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt atttgttttt
     9121 gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc atacacttca
     9181 agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt tttctcagca
     9241 ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg aaaagaggtg
     9301 ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc aaataaaggc
     9361 aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc gtaatattct
     9421 tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac ttgtcccatt
     9481 cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac tgctaattgt
     9541 tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc gatccttctt
     9601 tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac tgcagctccg
     9661 gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt ttgaggtcta
     9721 ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc agcaaccttt
     9781 ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca ttttagattt
     9841 acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga atgccaagag
     9901 cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc tttctggccc
     9961 tggattttct actcctacga tctgaatttc ttcattatag cttccataag agatgataaa
    10021 ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca tggtctcttt
    10081 catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc catctgcgac
    10141 aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg attcataaac
    10201 agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt cattccacaa
    10261 ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt caaactttct
    10321 tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa attcatcatt
    10381 tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta gttctgtgga
    10441 aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta atctacctaa
    10501 agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa acccgttgct
    10561 tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg ctcgcacttc
    10621 tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt tgtaattttt
    10681 gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag gtgctgtgat
    10741 cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt cttgttctcc
    10801 agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca tctgtttatc
    10861 tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg tgtctttcca
    10921 tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca tcataacaac
    10981 cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga aaagatagac
    11041 tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata ccgcttctcc
    11101 agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa accaactttc
    11161 tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt cttctcgttc
    11221 cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt caaacaaatc
    11281 aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc cgctattcgg
    11341 tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca cttcacttgg
    11401 tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc ctattcccgc
    11461 agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca tttttttctc
    11521 cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct agtaaagaaa
    11581 tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat tgacctggat
    11641 tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc tcgttataaa
    11701 cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct aattcatcca
    11761 tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc caaaattcag
    11821 ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc acacctttta
    11881 attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta acaaggccct
    11941 ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg tctgctggtt
    12001 tgatccattg atttttcaag tcattagcag gagccatatc taaataatat ttcccccctt
    12061 ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt tcagttgttc
    12121 ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca tttttaggat
    12181 agatatgaat ctcttgcagt tcctcgcttg attctgtcgg gtgataaatt ggcaaaatag
    12241 cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct ggatcaatcg
    12301 attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt gctttgatac
    12361 gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc gaagtcccta
    12421 tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc gtttcagcga
    12481 tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca tgataataat
    12541 cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt gaaatgatat
    12601 ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg tattcaaatt
    12661 gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc acgacatctt
    12721 cccctgattc ttctgccgac atcgcctgtg gaagaaggac tgctgcaagc ggtagtaaaa
    12781 caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct cctttttaga
    12841 ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg cagcaagcag
    12901 gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag gatcgttccc
    12961 gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta actcttgctg
    13021 tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt ttagtgtacc
    13081 cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc gataactaat
    13141 ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca cttccttgat
    13201 ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt ttggatctgg
    13261 tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct caattgaaaa
    13321 ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt ccaaatcgat
    13381 tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt catagccttc
    13441 cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact taccttcttc
    13501 attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg cgacagattc
    13561 cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta aatcgacata
    13621 cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac gtttcgattc
    13681 ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag aatcccccac
    13741 tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta attttctaac
    13801 tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata aacgatcctc
    13861 ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct tgagttggat
    13921 agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat caggttcttt
    13981 gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg tagtttcttc
    14041 actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt tctcaccatc
    14101 cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc ctttctcttc
    14161 ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt ccagtccgtt
    14221 accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac tgccttcaat
    14281 tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca gtttgacata
    14341 caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa gcttgtgata
    14401 ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc tcgaaccaga
    14461 tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg ctgcatattt
    14521 tttgatttgg aattgatacg tctcatatac taactgataa cgtggtggtc cagcgatctc
    14581 tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg gcatcttcgt
    14641 ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt cggcttcccc
    14701 actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct cgccattttg
    14761 acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg aagaagatgg
    14821 gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct gaatataggc
    14881 catattgttc actgtgtaaa aatttccgtc ttgagctccc gcatcaagtt ttacttggta
    14941 cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat aagcttggtt
    15001 ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt gtccatgacg
    15061 ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg gatctagatg
    15121 gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa aaccagtttc
    15181 atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat cctccaaggc
    15241 tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc catcttcttc
    15301 taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt gtctcccgct
    15361 aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct cttgatactt
    15421 gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt cttgcgaagt
    15481 aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag tcgctttatt
    15541 atctccttct tttacctctc cataacgata gtgtacattt acttcatctt ctggacggtt
    15601 aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat tcccagaaaa
    15661 ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt ccatcacagt
    15721 ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg gtgcataact
    15781 gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc tattttctcc
    15841 gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa gcggagttcc
    15901 tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat tgatatcatt
    15961 tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg tactattatg
    16021 gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca gatctttact
    16081 catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg tcccagcggt
    16141 taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg gactgatttc
    16201 ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg catcttcagg
    16261 atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt tgatgtacgc
    16321 cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa gctgaacggt
    16381 ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag tagcatagtc
    16441 gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca agttctctcc
    16501 gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc aatttcctaa
    16561 cacttttgta tagtcattcg ttgtaaattt cgggtagcga tgctgttcgt tttcaacttc
    16621 tccgctagga tagatatatt cataggggtc ttgcgtagct gttggcccaa gacgctccaa
    16681 aataccgttg aataactggc gttgaatgcg ctggagtgct tgaccgctgg gtacgattgg
    16741 ttcgtccagc ttttccatct cattttcttc gctacgttga atttcagcgg aatcttctgt
    16801 atcagtttca ttttcttgta attcttggtt atctattctt tcatcaacca ccacttgaag
    16861 aggtagttcc ccttcgttca catcggggat cttttctgtt tcaaaaatca atgtgccggt
    16921 ttctgcttct tttgaataaa cttttccgac ataccattct tgaccatcga tcgtctcgac
    16981 ttttaggtct tctccatctt catcatagga ggatagacct gttccacgaa cgttttttac
    17041 ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt attctacctt cattggcact
    17101 ttcctttttt tcaaaagcca gatgccattc aagggtttct tctttttcaa tatacttccc
    17161 ttcaattttt gcatagtcct catctaagaa tacctcatcc tcctttgttt ctgttgactc
    17221 agcagctaca tccagggtcg tcatatgata gactgactgg aaatatggtc caaatagcaa
    17281 actgacagtt actaactgca aaagcttcaa ttttaaccct ttcacatcct tatactcctt
    17341 tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca atgcccatct aattataat
//
LOCUS       DADXTG010000052        17224 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.053, whole genome shotgun sequence.
ACCESSION   DADXTG010000052 DADXTG010000000
VERSION     DADXTG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.053"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            252..743
                     /gene="nrdR"
                     /locus_tag="IWI57_001471"
     CDS             252..743
                     /gene="nrdR"
                     /locus_tag="IWI57_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBC2449622.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            766..2139
                     /locus_tag="IWI57_001472"
     CDS             766..2139
                     /locus_tag="IWI57_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBC2449623.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2139..3065
                     /gene="dnaI"
                     /locus_tag="IWI57_001473"
     CDS             2139..3065
                     /gene="dnaI"
                     /locus_tag="IWI57_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBC2449624.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3194..3634
                     /locus_tag="IWI57_001474"
     CDS             3194..3634
                     /locus_tag="IWI57_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBC2449625.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3778..4479
                     /locus_tag="IWI57_001475"
     CDS             3778..4479
                     /locus_tag="IWI57_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBC2449626.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4476..5597
                     /locus_tag="IWI57_001476"
     CDS             4476..5597
                     /locus_tag="IWI57_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBC2449627.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5612..6844
                     /gene="pepT"
                     /locus_tag="IWI57_001477"
     CDS             5612..6844
                     /gene="pepT"
                     /locus_tag="IWI57_001477"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBC2449628.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6976..7884
                     /locus_tag="IWI57_001478"
     CDS             6976..7884
                     /locus_tag="IWI57_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBC2449629.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7919..8191
                     /locus_tag="IWI57_001479"
     CDS             7919..8191
                     /locus_tag="IWI57_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBC2449630.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8202..9248
                     /locus_tag="IWI57_001480"
     CDS             8202..9248
                     /locus_tag="IWI57_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBC2449631.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9499..12108
                     /gene="clpB"
                     /locus_tag="IWI57_001481"
     CDS             9499..12108
                     /gene="clpB"
                     /locus_tag="IWI57_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBC2449632.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12259..12945)
                     /locus_tag="IWI57_001482"
     CDS             complement(12259..12945)
                     /locus_tag="IWI57_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449633.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(12923..13417)
                     /locus_tag="IWI57_001483"
     CDS             complement(12923..13417)
                     /locus_tag="IWI57_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBC2449634.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13437..14657)
                     /locus_tag="IWI57_001484"
     CDS             complement(13437..14657)
                     /locus_tag="IWI57_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449635.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14804..16639
                     /gene="lepA"
                     /locus_tag="IWI57_001485"
     CDS             14804..16639
                     /gene="lepA"
                     /locus_tag="IWI57_001485"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBC2449636.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            16835..>17224
                     /locus_tag="IWI57_001486"
     CDS             16835..>17224
                     /locus_tag="IWI57_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2449637.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKE"
BASE COUNT         5795 a         3059 c         3623 g         4747 t
ORIGIN      
        1 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
       61 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
      121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
      181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
      241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
      301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
      361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
      421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
      481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
      541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
      601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
      661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
      721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
      781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
      841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
      901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
      961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     1021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     1081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     1141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     1201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     1261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     1321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     1381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     1441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     1501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     1561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     1621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     1681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     1741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     1801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     1861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     1921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     1981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     2041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     2101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     2161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     2221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     2281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     2341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     2401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     2461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     2521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     2581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     2641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     2701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     2761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     2821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     2881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     2941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     3001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     3061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     3121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     3181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     3241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     3301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     3361 tgaaagtatt actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     3421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     3481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     3541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     3601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     3661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     3721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     3781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     3841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     3901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     3961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
     4021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
     4081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
     4141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
     4201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
     4261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
     4321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
     4381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
     4441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
     4501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
     4561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
     4621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
     4681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
     4741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
     4801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
     4861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
     4921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
     4981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
     5041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
     5101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
     5161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
     5221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
     5281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
     5341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
     5401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
     5461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
     5521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
     5581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
     5641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
     5701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
     5761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
     5821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
     5881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
     5941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
     6001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
     6061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
     6121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
     6181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
     6241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
     6301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
     6361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
     6421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
     6481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
     6541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
     6601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
     6661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
     6721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
     6781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
     6841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
     6901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
     6961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
     7021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
     7081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
     7141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
     7201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
     7261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
     7321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
     7381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
     7441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
     7501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
     7561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
     7621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
     7681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
     7741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
     7801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
     7861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
     7921 ggatgtattg ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt
     7981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
     8041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
     8101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
     8161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
     8221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
     8281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
     8341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
     8401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
     8461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
     8521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
     8581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
     8641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
     8701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
     8761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
     8821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
     8881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
     8941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
     9001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
     9061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
     9121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
     9181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
     9241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
     9301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
     9361 taagatttat ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat
     9421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
     9481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
     9541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
     9601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
     9661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
     9721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
     9781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
     9841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
     9901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
     9961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    10021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    10081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    10141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    10201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    10261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    10321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    10381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    10441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    10501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    10561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    10621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    10681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    10741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    10801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    10861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    10921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    10981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    11041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    11101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    11161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    11221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    11281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    11341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    11401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    11461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    11521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    11581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    11641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    11701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    11761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    11821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    11881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    11941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    12001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    12061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    12121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    12181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    12241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    12301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    12361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    12421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    12481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    12541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    12601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    12661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    12721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    12781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    12841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    12901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    12961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    13021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    13081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    13141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    13201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    13261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    13321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    13381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    13441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    13501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    13561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    13621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    13681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    13741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    13801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    13861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    13921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    13981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    14041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    14101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    14161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    14221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    14281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    14341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    14401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    14461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    14521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    14581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    14641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    14701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    14761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    14821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    14881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    14941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    15001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    15061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    15121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    15181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    15241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    15301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    15361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    15421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    15481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    15541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    15601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    15661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    15721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    15781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    15841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    15901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    15961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    16021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    16081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    16141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    16201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    16261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    16321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    16381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    16441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    16501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    16561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga
    16621 gcaagatagt gtgaaataac ttataatcaa tcgaaaaacc tattaaatca acctttttga
    16681 gtagatgcaa cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac
    16741 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
    16801 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
    16861 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
    16921 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
    16981 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
    17041 gattgagatt ctcatcggtc aactaattgc atacagagat atgaataagt tacttaacaa
    17101 agagttagct gataatatag gaattagtga agtattactc agttatgtgt taaatagaaa
    17161 gagaaatccg tcaaaagaaa tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa
    17221 agaa
//
LOCUS       DADXTG010000053        16648 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.054, whole genome shotgun sequence.
ACCESSION   DADXTG010000053 DADXTG010000000
VERSION     DADXTG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.054"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="IWI57_001487"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="IWI57_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBC2449638.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="IWI57_001488"
     CDS             complement(680..1666)
                     /locus_tag="IWI57_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBC2449639.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="IWI57_001489"
     CDS             complement(1689..2255)
                     /locus_tag="IWI57_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBC2449640.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="IWI57_001490"
     CDS             complement(2269..2685)
                     /locus_tag="IWI57_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBC2449641.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="IWI57_001491"
     CDS             complement(3142..5076)
                     /locus_tag="IWI57_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2449642.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5357..6313
                     /locus_tag="IWI57_001492"
     CDS             5357..6313
                     /locus_tag="IWI57_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBC2449643.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="IWI57_001493"
     CDS             6403..7506
                     /locus_tag="IWI57_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBC2449644.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="IWI57_001494"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="IWI57_001494"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBC2449645.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IWI57_001495"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IWI57_001495"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBC2449646.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12140)
                     /locus_tag="IWI57_001496"
     CDS             complement(10887..12140)
                     /locus_tag="IWI57_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBC2449647.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="IWI57_001497"
     CDS             complement(12146..13069)
                     /locus_tag="IWI57_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBC2449648.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="IWI57_001498"
     CDS             complement(13290..15782)
                     /locus_tag="IWI57_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBC2449649.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="IWI57_001499"
     CDS             complement(15789..16256)
                     /locus_tag="IWI57_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBC2449650.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="IWI57_001500"
     tRNA            complement(16392..16465)
                     /locus_tag="IWI57_001500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16556..16629)
                     /locus_tag="IWI57_001501"
     tRNA            complement(16556..16629)
                     /locus_tag="IWI57_001501"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16593..16595),aa:Pro,seq:tgg)
BASE COUNT         4927 a         3479 c         2959 g         5283 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg
    16561 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
    16621 tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DADXTG010000054        16578 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.055, whole genome shotgun sequence.
ACCESSION   DADXTG010000054 DADXTG010000000
VERSION     DADXTG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.055"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(22..918)
                     /locus_tag="IWI57_001502"
     CDS             complement(22..918)
                     /locus_tag="IWI57_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2449651.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(911..1591)
                     /locus_tag="IWI57_001503"
     CDS             complement(911..1591)
                     /locus_tag="IWI57_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2449652.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1592..2374)
                     /locus_tag="IWI57_001504"
     CDS             complement(1592..2374)
                     /locus_tag="IWI57_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449653.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2367..3284)
                     /locus_tag="IWI57_001505"
     CDS             complement(2367..3284)
                     /locus_tag="IWI57_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2449654.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3448..3771)
                     /locus_tag="IWI57_001506"
     CDS             complement(3448..3771)
                     /locus_tag="IWI57_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449655.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4559..7786
                     /gene="fss3"
                     /locus_tag="IWI57_001507"
     CDS             4559..7786
                     /gene="fss3"
                     /locus_tag="IWI57_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBC2449656.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7899..8213
                     /locus_tag="IWI57_001508"
     CDS             7899..8213
                     /locus_tag="IWI57_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBC2449657.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8226..8600
                     /locus_tag="IWI57_001509"
     CDS             8226..8600
                     /locus_tag="IWI57_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBC2449658.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8610..8945
                     /locus_tag="IWI57_001510"
     CDS             8610..8945
                     /locus_tag="IWI57_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBC2449659.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9028..10368
                     /locus_tag="IWI57_001511"
     CDS             9028..10368
                     /locus_tag="IWI57_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBC2449660.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10446..11120
                     /locus_tag="IWI57_001512"
     CDS             10446..11120
                     /locus_tag="IWI57_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBC2449661.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11113..11277
                     /locus_tag="IWI57_001513"
     CDS             11113..11277
                     /locus_tag="IWI57_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449662.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11353..12495
                     /gene="dcm"
                     /locus_tag="IWI57_001514"
                     /pseudo
     CDS             11353..12495
                     /gene="dcm"
                     /locus_tag="IWI57_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12485..12775
                     /locus_tag="IWI57_001515"
     CDS             12485..12775
                     /locus_tag="IWI57_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449663.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13034..14218
                     /locus_tag="IWI57_001516"
     CDS             13034..14218
                     /locus_tag="IWI57_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBC2449664.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14215..14352
                     /locus_tag="IWI57_001517"
     CDS             14215..14352
                     /locus_tag="IWI57_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBC2449665.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            15098..>16578
                     /gene="ltrA"
                     /locus_tag="IWI57_001518"
     CDS             15098..>16578
                     /gene="ltrA"
                     /locus_tag="IWI57_001518"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBC2449666.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT         5456 a         2972 c         3478 g         4672 t
ORIGIN      
        1 gttctcacta atcttgtata attaaaccct ccgcaacttg acggagatgg agaagatgcc
       61 cccgtctaat ctagcggtca tctctcctcc cattccttcc accaattttt ttgacaggta
      121 aagcccgatt ccgctagatt ccgtatttgt gcggctggta ttttcggtat aaaaacgctg
      181 gaagactttt tcaacagagg tttgcgttcc tggtttgata ggattggcca ctgttaatcg
      241 aatgaatgga tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa
      301 attctgtatg atattttgca gcactcgcag caacatgagc ggatctgcta atacagttgt
      361 atttatcgag tgttcagcga acgctacctc gatatttttc tcagaaaatt gttcataaaa
      421 agagaaaaat acttcttcca cctctttaga aaaagaaagc gtatggagat ccatttcttt
      481 ttgcttcgtc tctattcgag caagatcata aaactgatcc gtcatactga gtaatcttgc
      541 aacagattgt ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct ctacctgttc
      601 cagtaattgt acataaccgt taatagcagt caaaggagtt ctgagatcat gagaaaggcc
      661 gacgatcgat agtctgatgt tttcttccat ttcttgaaat gcttgtcctt tttgttcatt
      721 ttcatccaac catgtattca gtgcatctac taggtccatt agattctttt ctcgaaattc
      781 gactgaaaga cgaccaccat attcttgtcg ttcagaaaga gtggcgattt tcttagttaa
      841 tctcttgatt tccctttgca ggaagaaaat aaaacttccc ataaagataa caacgagaag
      901 caacagccat ttaatcaaat ctaaacccca ctccccacac cgtatgaata tacacagcat
      961 gtgcctttgc tagcttccct cgaagattgg aaatatgcat attcactgtt ttctcactat
     1021 caaaatatgg ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat acttttttcg
     1081 gatgttctaa aaaaagcttc agcagatcga attctcttcc tgtcaaatgg acagccacac
     1141 cattcacatt tacatccctc gactctttgt ccagtaagat ttccttgtaa tagatgcgtt
     1201 gcgacagatc ttttacttgg acagaagtct gatgacgaag ttggatgccg attcgtaaca
     1261 aaagttcttc gatatcaaat ggtttcgtca catagtcatc cgctcctgct gccaacaagg
     1321 aaactttatc tctttgatcg ctttttgctg aaagaacgat gatcggaaca ttcgatgtct
     1381 gtcggatttc tgctaacaat gcttcacctg aaagccctgg aagcatgaga tctagaagga
     1441 tcaaatcgac aggatatgtg cttaacaata attttccttc cgtacctgaa taagcatcaa
     1501 gaagtttgta tctctgttca agaacctcct ccagtaagga atgaacagct tcatcgtctt
     1561 cgataatcaa aatagtttcc agcaggacca cctacttaat gtcagatttt tggaaaataa
     1621 acattcctag agctgatgat accacgattg ttactagact gaccagaatc attttcggtg
     1681 cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc
     1741 actccgttag aaattccaag cctttgattt ttgcgaaaac accgggaatg aaggccgttc
     1801 ccatgatata aagaaaagaa agaagcaaac tgacactggt acttctagtg atgaatgtca
     1861 aagccactgg aacagataat gtccctaatg tcagcagaag caatacagga aatactttta
     1921 gaacataatt tccatagttt ttgattccct caacttcacc aaatccgaaa gcaagtgtgt
     1981 aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa
     2041 cgagaagttt cgacacataa taagtcattc gattccgtcc taaagataac gtattgcgaa
     2101 tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc aaatgctgcg aaagccagaa
     2161 gaatccatgc agccattttt tctaccatta aaatccctgt tgcaccgttg atgactgtat
     2221 ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat ataccattcg ttccaagaat
     2281 aaaaaagcgg aagtacgacc atcagtccta gtgagaccca cattttacgg ctcttccata
     2341 cttttatttt ttcagcagtt aatagattaa acatgtggat tgcctccagt taattgtaag
     2401 aaataatctt ccagtttttg cccaactagg tgaataccat ccacaggaac ttttgcttct
     2461 accaatgctt gattgatggc tgctacttgg tcaattcctt caaaaagctg gataacacgg
     2521 ctagattgta caaaataatc cctataacca agagagtcga gaaccgttac tgcttgttcc
     2581 gtcgccgaag tgtcaatttt tataaattga cgagattctt gtgccaattc ttctttcgat
     2641 aattctttga tcaagtgtcc ttcatgcaaa ataccatagt gagtggcgat ctgtgacaat
     2701 tcatctagca agtggctaga aagcaagacg gtgatgcccc gttcttttac caaacgttgg
     2761 atgatttctc gcatttctgc catccctgct ggatccatcc cattcgtcgg ttcatccaaa
     2821 atcaaaaatt ctggattcgt gatcagtgca tttgcaatag ctaaacgctg gcgcatacct
     2881 aaagagaaat tctttgcttt ctttttccct gtattttcca atcgcatcat tttcagcagg
     2941 tcttcgattt ctctatccga cacaccgccg ttcgctgctt ggatacgtag gttatctctt
     3001 gcagttaatt ctggataaag agcaggggtt tcaatcgatt gacccatacg tcgtcttgct
     3061 gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt catcaatcga gatcaaaccc
     3121 ataatagttc tcataaaggt agacttaccc gctccatttt ccccaatcaa tccatagatc
     3181 attccacgtt tgatttcgat tgatacttta tctaatgctt tggcagcacc gtattttttg
     3241 gttataccag ttgctttcaa gaccgtttca tttccaaaat ccatacttat ccttctttct
     3301 tattgatgag tgaagtatag ttgacgggta taaagaaagc agccagaaat gtataaagaa
     3361 agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg acaatacgtg tctttcctat
     3421 tatcaggcag tttgttatac gcttgtatca gtttcctgat tctttctttt cgaccaaagt
     3481 cgtttctctg tattcctctt cactgaaaat ttttgcttct ttcagatcaa gcttgagtaa
     3541 ttttaccaga ccggttataa ggataactat agcaagactc cctcccagaa aaattagact
     3601 gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc gcaccaaaca gtgctaatcc
     3661 aagtggactg gccaccatgg taatcgttgt tatcgttgac ataagtcgtc cttgtatatg
     3721 cgttggaact tctcgctgaa gccagaccat ataaaaagca aaagaaaaca ttaatcctta
     3781 aaaacggact tatgaaaaat aatacatgcc gtttgggatt tacaattagc taccaaataa
     3841 gaggttatga caaaagactt ttcacaatcc cttcttccga ataaacggtg ttctcctgcc
     3901 aactcctcgg tcacaagtca caatatttat caaacatgag aagcaatttt tgtaaattcc
     3961 ttcttatttc ttgaagctga ccacttctgt cacaacctca gaagcggata aataatattg
     4021 tttgatgata ctttatcttt ttcccgtttg tttatagatc gaatgatatt atagaccaag
     4081 aatcctacct attaacatag ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt
     4141 gccagaaaag ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc
     4201 gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg aagctttacg
     4261 ggctttctta agacctggtt ttttcttcgt atcattgaat aagtttgaaa aaccttgatt
     4321 tataaaggtt ttcccgtaaa tatattttat tatatttgat taaatttgaa atgtttgtgt
     4381 tctaaaatgt gttctaattt aaaggcagta tatttcgtat tttagattaa atttttaggc
     4441 tcattcattc tcaaatccat tctattcagt caataaaaat tttaaaaaga ctttataaac
     4501 aaagatgttc aagcgagcat cttatttttt tgtgcttaat ttgaaaggag aacatttcat
     4561 gtataaaaaa caaaaaaaga aattatgtgt attaactatt ttgagtttgg ctgttcaagc
     4621 aattgttgga gcaattgctc ctacgattac ttttgctgat gaaattaccc atccacaaac
     4681 ggtaaccgta gaactagatt tagcacatca atatgctgtt gaaggaactt ttagtgatgg
     4741 tcgtccaatg tcagaagtca ctgttccgca ttatgcagtg tacaacggct ttaaacaaga
     4801 tattttctgt attgaaccag gagtcccgat ttacaatgag ttcactcctg gctatgagaa
     4861 aaacccgtta ccagatatgt ccgataaagc caaattagtt tccgttttat ggaaaaaagt
     4921 aggtacagat gcagatactc atattgtggc acagaagatg atctggcaag aagttaatgg
     4981 gtataccctt cactcaataa aacgatcaga tggtagtgcc gtaaatatcg cagccattga
     5041 ggcaaaaata aatcaagcca ttgacgatta tcagaagaaa ccaaccttcc ataatagtac
     5101 ggctaaaaca gtattagggc aatcgactac tgtgacggat acgaataatc tgaatttgtc
     5161 agagttcgat gaggttgtgg aaaatgccgc aaacattgat tatcgtgtga atggtaatca
     5221 gttgataatc accccaaatg ccaattcaaa agaaagtggt gtgctgacgc ttaagaaatc
     5281 tgctggtacc ggaacacctg tcgcctataa aaaggctggc caacagactt taatggccgg
     5341 ggcgattgat aaacctaata cctacacaat caaaattgat gtggaaacgg agggttcttt
     5401 aaagatcaaa aaagtcgata aagaatcggg tgatgttgta ccaggaacgg ttttccattt
     5461 agactttgga aaaactttac ctgcaaaaga cgtgactact gacaaagaag gcattgctac
     5521 attggatgag attcctcatg gtacaaaagt aaccattacc gaaaaatcag taccggcgcc
     5581 ttatacgatt gataccaccc ccatgactac caccattaaa gcaggcgaaa ccatttacgt
     5641 gacttcaaaa aacgcacggg aaaaagggca aatcattctg gataaaagcg gagttgaaac
     5701 agggagtgat ctttggaatg acaattattc cttagctggt aatacgtttg ccattcgcaa
     5761 agacagtcca accggtgaaa tcgttcaaga aatgaccacg gatgaaaacg gtcatgcgga
     5821 aacaccaaaa gagattgcta atgcgttgga attgggaacc tattatgtga ccgaaactaa
     5881 agctagtcat gggttcgtga atacgttcaa acctgtaaaa gtcgaattga agtatgccaa
     5941 tcaaaccgta gcccctgtta ccagtaacgt aaaagggcaa aatcaagaag tcactggcga
     6001 aaccactttg acgaaagaag acaaagatac tggggataaa gcacaaggta aggcagtctt
     6061 tgagggtgct gaatacacct tgtttactgc taaggatgga aaagctgtta aatggagtga
     6121 ggcttttaaa ccagagttag cgaagggaac gaaaggttct gatgaaacgg tcactttgac
     6181 tttagacgaa aagaaccaag ttgccgttaa acatttagct atcaacgagt attattggca
     6241 agaaacaaaa gcgcccgaag gatatagcct tgatgaaaca aagtatcctg tatccatcaa
     6301 aaaggtggat gatagcgaaa aaaatgccgt gattacccga aatgttacgg caaaagaaca
     6361 agtgattcgc tttggctttg atttctttaa atttgctgga tcagctgcgg gcactaccga
     6421 aactggcttc aatgatctga cctttaaagt gtcgccattg gaagggacca atgaaatcac
     6481 aggtgccgaa gatgaagcga ccacagctta taacgagcaa ttagggtttg atggttatgg
     6541 caagtttgaa aatcttcctt atggggatta tttacttgaa gaagtagaag ccccagaagg
     6601 ttttcaaaag attacgccat tagaaatccg ttctactttt aaggaaaaca aagaagactt
     6661 tgtgaagagt gagtatgtct ttaccattac cgaacaagac caaaaacaac caattaaaac
     6721 ggtgaccgtt ccttacgaga agctgacgaa caaagcattt tctgttagtt tgaaccgttt
     6781 aatgctctat gatttgcctg aggaagaaga tagtttaact tctcttgcga cttggaaaga
     6841 cggaaacaaa gaattaactt ctctggattc taccgagcta gttgataaat tgagttacaa
     6901 tctacatgaa atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggatgcgac
     6961 aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg gtgattgcag aaacaagcgc
     7021 aacgttggcg aacaaagaga aaacaggaac ttggaaaatt cagcataaat taaccgccga
     7081 acaagtttta aataaaacta ttgtcttatt caactatgtg tatgaaaata aggaagcttt
     7141 tgaagcaggc gataagccag tagcgaagga tgttagcttg aataaccaag cccaaactgt
     7201 cagttgtaca gtggaacacc atgtttctat ccaaacaaag gcccacctag aaaacggttc
     7261 tcagaccttt actcatggtg atgtggtgga tatgtttgat gatgtgtcga ttactcatga
     7321 tgtactggat ggctcaaaag aagctttcga aacaattctg tatgctttat taccggatgg
     7381 tacaaacaaa gaaatttgga aatctggcaa gattgattat gaagtaaatg ataaagaatt
     7441 caccaaaact gtgcttgcga aaaaagtaga taccggaaaa tatccagaag gaaccaagtt
     7501 tacttttgcg gaaatcaatt acgataaaga tgggaatatc aatggaaaac acaatgaaga
     7561 tttgaaagaa aaatctcaaa ccttaacacc aaaagaagtt ccaaccatac taagtacgcc
     7621 gaaacaaccg gaaacaccaa ctgttccaag tggctctcaa gaatctagtc ccacagtgaa
     7681 gaccttccct caaaccgggg agaaaaattc caatgtttta ctgttcattg gttttacttt
     7741 gatttttgcg acggcgggct attatttctg gaatcgccgg aactaaggtg atgagatgat
     7801 acaagtaaat cctaagaacc aacgtgcgcg accgcctcct aaaaaagtag tcgcgctatt
     7861 tttgtatcca aaactaacaa tgaaaagagg aaaagaagat ggaattgaaa tttgtcgtgc
     7921 cagatatggc tgaaaccttt ggaaagttaa gttatgccgg tgaaggagaa attataacag
     7981 aaggatatgg acggaatact acggtgattg gtcgcagtta tcatttgtat tccagtaagc
     8041 aacgggccga tgatattgag gtggtagtag ctgcggaagc cggtgaaaag gattttgatc
     8101 aagaccaacc gctaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
     8161 atcgtgggtt tactgattac gtgttgtatg ttgatgattt agtgaaagca taggaggaaa
     8221 agaagatgag attaacagaa ggtattgtcg tagattcagg tttaacattt gggaagttgc
     8281 gtttttccgc attacgccga gaagtacgga agcaaaatga ggatggaaca attggtaatg
     8341 aggtaaaaga acgaacctat aatttaaaat cctctgcaca agggcgaatg attcaagtta
     8401 gtatcccagc taatgtaccc ttacgcgagt ttgcttatga tgcagaggta gagttggtca
     8461 atccaatcgt ggataccgtt gccaattatg tttttcgaga aggaactacc gttaattggt
     8521 tcattaaggc ggatgattta gtcttgaagc ggcagccgaa tcaaggaaat cctacgaacc
     8581 aaaatgaaag aaagaaatag gtgaagatta tggaaattaa tgttgaacat attttagatt
     8641 gtctcgatca atatggaaaa ggcgagctga ctgaggaaca actcgttaaa gcattaacct
     8701 ctgatgaaaa aatgtttctg attatacatc aaggtttact tggagaaaat agcgacagtg
     8761 aagaagactc tgatgtacta gactggttag gagaagaaga gtctttcttt atagttgtcg
     8821 aagtaaatga aaacttatgt cggcaggcag aatccatttt ggaagaaatt ggtgtggaga
     8881 tgccggatgc tattgaaggc tttttaaatc aattggttga aacgaaacaa ctcccctcct
     8941 cgtagcagta aatgactagc ataacttttt gaaattgcct aatgaaatcc tattgggctt
     9001 tttcgattgg aggtggaaac catcaaaatg tacaaaggac atcgcatacg cgcaggagat
     9061 cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat cggttggatg
     9121 agcgtctttt attttcaaga gtttagacaa tttgatattt ctaagctatc actttctact
     9181 attgaaatcg tttggtctat aaaagattta gtatgtctgc ttggaagtct cgctttttca
     9241 ggagctatga tattgcttta tattcacttt tttcttgatc attggcgaag cttgtggcat
     9301 cgacaaaagc tagctcggat gattctagag aaccattggt atgaagtgaa acaaactcaa
     9361 agtgaaggct ttttcaaaga tttgaatagt agccgaacca gagagactat cagttacttc
     9421 cccaaaatct attatcgaat gaaagatgga ttactgtcta ttcgcgttca aatttcactt
     9481 gggaaatacc aagatcagct tttaaaattg gaaaagaagt tagaaagcgg actgtattgt
     9541 gagttagtag aaaaggaact caaagactcc tatgtggagt atactttact gtatgatatg
     9601 atcgccaatc gaattgggat agacgaagta gtggcagaaa atggtactct gcgattgatg
     9661 aaaaatcaag tatgggctta tgattcttta ccccatatgt taatcgctgg tggcacgggt
     9721 ggagggaaga cctatttcct cctcaccatt atcgaagcac tattgaagtc agatgcagaa
     9781 ttgtttatcc tcgatcccaa aaatgcagat ttagctgatt taggtacggt gatgcctcat
     9841 gtttattcac aaaaggaaga aatttctgct tgtgtggaag atttctatga acgtatgatg
     9901 gctcgtagca aggcaatgaa agaaatgtct aactacaaaa caggagagaa ttatgcctat
     9961 cttggacttc cacccaactt tttaatcttt gatgaatacg tggcttatat ggagatgtta
    10021 acgacgaaag aaagtgcggt gattttgaat aagctcaaac aaatcgtgat gttgggtcgt
    10081 caatctggtt tctttctgat tttggcttgc caaaggccgg atgcgaaata tttaggagac
    10141 ggaatccgcg atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga actcggatat
    10201 tccatgatgt ttggggaggt cgaaaaaaac ttctttatga agcacatcaa aggtcgaggt
    10261 tatgtggata caggtggcag tgtgattagt gagttttata cgccacttgt accaaaaggg
    10321 tatgatttct tagattcaat tagtaaatta gaaaagatga atgattgagt gatcttcaat
    10381 actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga aaggggataa
    10441 ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag gataagttta
    10501 tttcttatat tatcaaaggg aaatcccccg aagttggacg agacgcagtt tttatggcag
    10561 ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat tatgataacc
    10621 atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat ggaaaaaact
    10681 ctagtctctt tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag
    10741 atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata
    10801 tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctatgtttat caagacccta
    10861 aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa
    10921 aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac
    10981 atccagatgt attgcttcat atcataataa aagattatac gaataatatt tccgaagttc
    11041 attatgctac ggataatcta gaattatttt taccactagg gtatagtagg aaagatttgg
    11101 aggttcaatt acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa atacaataag
    11161 agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt gattaaatat
    11221 gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa aaaatagtag
    11281 gacaaattaa taatgtatga gccaagagca aaatgttctt ggcttttttc tttgcaatga
    11341 atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt tcgtttaggt
    11401 atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt tgctagacgt
    11461 agttataaag caattcatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta
    11521 tcagatgagt ttattcaatc cctcggacca gtggacatcc tttgcggcgg atttccgtgc
    11581 caagcttttt caattgcggg aaagcggcaa agattttccg atactcgagg tactttattc
    11641 tttgaaatcg ctcggttcac cactcttctc cgacctaagc ttttattcct tgagaacgtc
    11701 cgaggattgc tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat
    11761 ggattggggt atgacgtgga gtggtaggtg cttaactcaa aggcctacat tccccaaagc
    11821 cgggagcgga tattccttat cggacattct cgagacgcat gtaccgaaca agtatttcct
    11881 atcattggac cgtcttcaac atctgatcaa aacatcagaa atttggtaaa tatcaatcct
    11941 tccaatcgag gaatgggtgg acaagtctat ggttcagatg gcgtagcgcc aaccttaaca
    12001 ggtgatgaag gaatcaaaat cgcactgcca gcaagggatg gaatctctgt agcaggacta
    12061 ttaccgggaa agtttgaaca aggtaatcga gtttatgaag ttactggaac cgcccccacc
    12121 ttgtcaacga aacaaggtgg tacaaaaatc atgattcgaa aaaatgaaac agaatatcaa
    12181 gaggtgaagc cagaagacag tgtgaatctg gcttttccta attccacaag tcgccgaggt
    12241 cgtctgggaa aacagagtgt tcatacctta ctaacaggag accaacaagc gatcgtcacg
    12301 gatcagtatc aaattcggaa gctaacacct agagagtgtt ggcgacttca gggctttccc
    12361 gattgggctt ttgatcgaga ttctcaagtc aattcagata gtcagttgta taagcaagcc
    12421 ggaaactctg taacagttcc ggtcattttt gatattgcca gaagattaaa agagttggat
    12481 gtacatggtt tatgatgagt tgattggaga aatttattgg gtgattggaa agattcagtc
    12541 agacccagaa ttagaagaac aattgcgacg gttaaaattt gatattcgca agaatggtgt
    12601 caaagtcgct ggcgatccat atttaatgaa tgaggagacg gatgctcgaa ttgaaataaa
    12661 tcaagtaatt gcagaatttg agcggattgc ggaccaagca aaagaaccag atattcgtca
    12721 atacttgtta gaaatgaaag cagagttgga agtaaatggt attacagatg agtaacctaa
    12781 accaacacaa ctgactgtta ggcgctgacc gcggaggatt cgcgaagcag aaatcctccg
    12841 tggtgctgtg cctgttcatg gacggcgtta gccgaccatg atgtctcacc cccgtatcta
    12901 acaggggggt acaaaaacac aaaaaataag taagcaacca ttaagaatgc ttgttgtatc
    12961 aaagatagta agtaatttgt ataggtgtta actcatttga aaaagttaac accttgaatt
    13021 tggaggtgag aacttggcac aacggaattt agattatcgg ctattaaaag accgacgcaa
    13081 cgaatacggc gtttcacaaa ataaattggc tacggcttgt ggacttagtc ggccgtactt
    13141 aaatcagata gaaaatggtg gcgtaaccgc atctataaaa accatgagga aaatttttga
    13201 ccaactggaa agctttaatc ccgatttgcc tttgacgtta ctgtttgact atgtaaggat
    13261 tcgctttccc acaacggatg cacgaaaaat tattcaagaa attctccatt tgaaatttga
    13321 gtatatgctc catgaagatt atgcctttta ctcctatcaa gaacagtatg tcatgggtga
    13381 tattgtggtg atgttgtctc atgaagaaga taaaggtgtg cttttagaat taaaggggcg
    13441 aggttgccga caatttgaaa cttttttact cgctcaaaag cgtagctggt acgacttttt
    13501 cgaggattgt ctaaaggccg gtggcgtgat gaaacgcttg gacttggcaa tcaatgatcg
    13561 agtggggcta ttggatatac ctgagctaac gaaaaaatgt cagaaggaag aatgtatctc
    13621 cttgtttcgt acctttaaaa gttatcgttc cggggaactt ttaaaggctg atgaaaagga
    13681 cggcatgggc aataccttat atatcggcag tctcaagagt gaggtttatt tctgtttgta
    13741 tgaaaaagac tatgaacaat atatcaagtt agggattcca ttagaccaaa ctgagacgaa
    13801 aaatcgtttt gagattcgac tgaaaaatga ccgtgctttt catgcgattc aagatttgtt
    13861 aaaaggtcgt agcattgaga gcaccacgtt ttccattatc aatcgttact tgcgatttgc
    13921 ggataaagta gaaggtaaaa ggcgaaccaa ctggcccttg aatgaacaat gggggcgttt
    13981 tattggacgg aatcggaaag agattcaatt aacttccgag ccaaaaccgt ataccattga
    14041 acgaaccttg aattggctag gccgacaagt cgcccctacg tggaaaatgg ctaaggagtt
    14101 ggatcgattg aatcggacaa cctatatcca ggatatggta cagaatgcac gcttatcaga
    14161 ccgacataaa aagattttgg agcaacaaag tatggcaatt gaaaatttga ttatatgaaa
    14221 agaggaatcg tcatgggaat tgtaaaagat atttttttgg tatttggtgg cagtatgctt
    14281 ggtgttactg tgatgtgctt gatgaatgca agtgcagcag cggatcgagc aatggaaaag
    14341 aaaggaaagt gacaagatga atttcggaca aaatttatgt gcgactcgtt ccttagtgaa
    14401 aagctaaggc actaataaaa gaacgaaaaa gtttggttat taggagaact gataatctga
    14461 caggtggaat gatgaagtaa cgctctgaaa cgcctgccct gagcctccga caagcattgt
    14521 atgaggaaac tcagaatgtt tgaagctcgg taaagacggc tgaaagatac cgtaatagtt
    14581 gatgaacata tatcttctat cgaaaactat ccagaggtgg gtagcgtatc caacaggtcg
    14641 ggggtctata aaatatctat ggttagaatg ttctataagg aactgacgaa acagcgaatg
    14701 tacgggtcta aatttgaact ttgtagaaat gcaaaggtac gagaaatcgt agctggtgtg
    14761 gcaaagtaag aatataggtt atgaaatgcc atataacgtt acaggcggta tcaagccagc
    14821 aggcttatag ctagtgacct aaggatatat atgtatagat aagattatcg gaacgaggaa
    14881 aggtattggg ttctcatcaa gagaataaac tgacgaagaa caataagcag ttgaactaat
    14941 gctgaaaagc agagggcgaa cctaagaaat tcttgtaatg agaatggagg aatgccctca
    15001 agtcgtgtat atattagaaa agtattatgt tgtttcaata aggattacga gtaagataaa
    15061 aaagctcact caatttcaag taaaggagtg aagccttatg cctaaaaata aaaatgaaac
    15121 attgtgtgta gaagatttaa gacacgctga atattacgaa atgcaaaaca cctttgatga
    15181 tttatatgcc aaaagtaaga atggagatat tttcactcat cttatggata ttatcctatc
    15241 gagagaaaat atattactgg catatcgaaa tatcaaagca aatgcaggca gtaaaacagc
    15301 aggaacagac ggtacaataa ttaaagatat tggcaaattg ccagcagaaa cagttgtcaa
    15361 aaaagtaaga tacattgttg caggcagtcc acatgggtat cgacctaagc cagtgaggcg
    15421 aaaagaaata ccaaaaccaa atggtaaaac cagacctcta ggtattcctt gtatgtggga
    15481 caggcttata caacaatgta taaaacaagt attagaacca atctgcgaag ctaaatttag
    15541 tgaaaacagc tatggtttta gacctaatag gtcagttgaa aatgcaataa aagcaaccta
    15601 taaccgccta caaatcagcc agctacatta tgtgattgaa tttgatatta agggtttctt
    15661 tgacaacgtg aaccattcca agctgataaa acagatatgg gctatgggaa tacgggacaa
    15721 gcacttgata ttcattttaa aaaggatatt aaaagctcca atcaaaatga ctaacggtac
    15781 aataacatat ccagagaaag gaactccgca agggggaata atatccccac tactagcaaa
    15841 cattgttctt aatgagttag accattgggt agaaagccaa tggcaggaaa atcctgttac
    15901 aaaaaactat gtggttcata tcaataaaag cggtagccca tgtaaaagca acgcttacaa
    15961 agaaatgaag aaaacaaagt taaaggaaat gtatatggtt cgttatgcag atgattttag
    16021 agttttctgc cgctacaagg aaagtgctga aaaagccaaa atagcaatca ctcaatggat
    16081 tgaacaacga ttaaaattgg aagtatcaca agaaaaaaca agaattgtaa atgttagaaa
    16141 aagatactcc gactttcttg gtttcaagat taaaatgata ccgagaagaa agaagttagt
    16201 tgttaaatca catataagtg ataaacaatt caaaaaccaa aaagacaagc tggtatcaca
    16261 ggcaaagaaa attgcttctc cctcaaaagg gaaaacggaa ttagaagaaa caagacttta
    16321 taattcaatg gtattaggta tgcaaaatta ttatcagatt gcaacgaatg taaatctgga
    16381 ttgtatgaaa ctgaatagag ctgtaatggc tatcttcact aacagattag gtacaaacaa
    16441 aaggggcaga ttgagaaaaa caggcagaca acttacgaca aaggaggcaa caagatatgg
    16501 taaatctaaa atgcttcggt atgttgctgg cgagggcgaa cctattttcc ctgttggata
    16561 tatccaacac agaaaacc
//
LOCUS       DADXTG010000055        16306 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.056, whole genome shotgun sequence.
ACCESSION   DADXTG010000055 DADXTG010000000
VERSION     DADXTG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.056"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="IWI57_001519"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="IWI57_001519"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBC2449667.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="IWI57_001520"
     CDS             complement(1061..1981)
                     /locus_tag="IWI57_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2449668.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="IWI57_001521"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="IWI57_001521"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBC2449669.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3833..4366
                     /locus_tag="IWI57_001522"
     CDS             3833..4366
                     /locus_tag="IWI57_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBC2449670.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4856..5380)
                     /locus_tag="IWI57_001523"
     CDS             complement(4856..5380)
                     /locus_tag="IWI57_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449671.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5373..5846)
                     /gene="tsaE"
                     /locus_tag="IWI57_001524"
     CDS             complement(5373..5846)
                     /gene="tsaE"
                     /locus_tag="IWI57_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBC2449672.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5969..6949)
                     /gene="pta"
                     /locus_tag="IWI57_001525"
     CDS             complement(5969..6949)
                     /gene="pta"
                     /locus_tag="IWI57_001525"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBC2449673.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7102..7782)
                     /locus_tag="IWI57_001526"
     CDS             complement(7102..7782)
                     /locus_tag="IWI57_001526"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBC2449674.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7908..8777
                     /locus_tag="IWI57_001527"
     CDS             7908..8777
                     /locus_tag="IWI57_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2449675.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8803..9318
                     /locus_tag="IWI57_001528"
     CDS             8803..9318
                     /locus_tag="IWI57_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449676.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9365..9958)
                     /locus_tag="IWI57_001529"
     CDS             complement(9365..9958)
                     /locus_tag="IWI57_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449677.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9983..10162)
                     /locus_tag="IWI57_001530"
     CDS             complement(9983..10162)
                     /locus_tag="IWI57_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449678.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10239..10748)
                     /locus_tag="IWI57_001531"
     CDS             complement(10239..10748)
                     /locus_tag="IWI57_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449679.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10844..11224)
                     /locus_tag="IWI57_001532"
     CDS             complement(10844..11224)
                     /locus_tag="IWI57_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2449680.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11554..12303
                     /locus_tag="IWI57_001533"
     CDS             11554..12303
                     /locus_tag="IWI57_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBC2449681.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12487..13764)
                     /locus_tag="IWI57_001534"
     CDS             complement(12487..13764)
                     /locus_tag="IWI57_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449682.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13843..14487)
                     /gene="pcp"
                     /locus_tag="IWI57_001535"
     CDS             complement(13843..14487)
                     /gene="pcp"
                     /locus_tag="IWI57_001535"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBC2449683.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14686..15630)
                     /locus_tag="IWI57_001536"
     CDS             complement(14686..15630)
                     /locus_tag="IWI57_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBC2449684.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15632..>16306)
                     /locus_tag="IWI57_001537"
     CDS             complement(15632..>16306)
                     /locus_tag="IWI57_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBC2449685.1"
                     /translation="LSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLEL
                     LGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMI
                     FAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQ
                     VLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKY
                     YPGKGQ"
BASE COUNT         4689 a         3384 c         2894 g         5339 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta
     3001 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
     3061 cagatacaag cttttgaaca ctgtttatcg agagattgtg aaaaagctgt cctgcatcaa
     3121 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt gggcttatta
     3181 ccgcagatac aagcttttga acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat
     3241 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttgggctta
     3301 ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtgaaaa agctgcactg
     3361 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttggac
     3421 ttattaccgc agatgcaagc ttttgaacac cgtttattga gaagttgtga aatcagcgtc
     3481 ctgacctgag tattagagaa acaaatggca aaatagttcc tcatattttg ctattttttg
     3541 agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagaagttgt gaaaaagtcg
     3601 cctactttcg agtcgtaaga aatttttttg aaaatggttc ctcacaactt tgcataaatt
     3661 tgacttaata ccgagaagtc atgatcccca tctaactcca aatcacaagg aatgagttca
     3721 aaaactagct tttctcaaca aatattacat aaaaaattct atgttttttg ttaaatttat
     3781 ttgctttttt caaaagtatg ttacagtaat ttcccaagga ggttttacag aaatgaattt
     3841 tgttgccatg gattttgaaa ctgccaatca tcagccttat agtgcttgtt ctcttgcgct
     3901 agtcatggta aaaaatagcc agatcgttga tgaattctat acattgatac agcctgagac
     3961 tccttttttc tggcgaaatg tccaaattca cggaatccat caagaagacg tacgcaatgc
     4021 gccgaaattc ccagaagtct ggcaaactat ccaaccatat tttcaaaaaa atcgtttagt
     4081 cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg ggatgcttag attattatca
     4141 tcttgaacag cctaattatc tttctttatg tacagtgaaa agtagccgtc gcctgtttcc
     4201 tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat ttgaacatcc aattagatca
     4261 tcaccatgat gcattggaag atagtcgtgc atgtgcggaa atcctgcttt atcaagaaaa
     4321 aaaattcggg acagatccat tgaaaaaact tgtgactata aagtaaaaga atacttatga
     4381 aacacacaaa gaggtcgtga gcctgccgtt tcactccgag tcacaaggag caatttcaaa
     4441 aaatagcttc tcatgtttat taaatattgt gactggtgac cgaggagctg gcaggagaac
     4501 accgtttatt ggagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
     4561 caaaaatagc ttctcatatt tttcatttgg ttggacttat taccgcagat gcaagctttt
     4621 ggacaccgtt taccaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
     4681 acggcaaaat agttcctcat attttgccgt tttttgagct aataccgaag gtcagctgat
     4741 tgaacaccgt ttattcaaaa aaaatggact gcgacacgac ttttgtcaca gtcccttcgt
     4801 gtgtttttct gccttctcaa aaggatgaca gtttctcact cttttcctat ttcttctatt
     4861 caatcaaata actcatttta tatacatcta aaaattcacc tagatctgtt cttgcccctc
     4921 taggcatgac cgcttcgatt tggaagccca tttttcggta aaggtgtacg gctctttcat
     4981 tacgaacttg aacgtcaagc tccaatcgaa aaagtacacc catttccttc gcccaactga
     5041 tgatttcttc tagcataaga gtgcctagtc ccattcccca atattcctgc agaatactga
     5101 taccgacttc tcccacgtga gacaaacgaa attgactgtc tgccttgaca gatgctgttc
     5161 caataatcgt cccttcatag attgcaagca gcaataaatt attattgctt tcgtatatat
     5221 aatccaactc ttcttctaag gtttctggtg gtaacaggag ttctgcttca tccaaaacta
     5281 aaaattctgt ctccttatta acttgtccca tcatcaaaag aatgtctttt gcatctcctg
     5341 gcactgcttc ccgcaagatg atcccaactt cttcactcat gattttcagc ccatttttct
     5401 agaattcgct gtttgaaggt ttcactttgc tccccgtatg agattagttt cacataccgc
     5461 ttttctaaat ctgtatcact tttttctaaa atcagttcca aataatcctc cggcagtgct
     5521 tcttctaata gatttcccca ttcaacaacg gaaagcccat caccttcaaa atattcatct
     5581 aaccctaaat ctgctccgct agctgctacc cgataaatat ccatatgata caacggaatc
     5641 cgaccttgac tatattctct aataatcgtg tatgtcggac ttttgatcat ttgcgttatc
     5701 cctaatcctt gggcaattcc tttcgtcaac gttgttttac ccgcacctag atcaccagtc
     5761 aatatcagat tgtcacctgg cattgcggaa tgaccaattg cctctgcgaa ttgttctgtc
     5821 attgttaatc ctgatagttc gatcattttt catcttctcc cgctcttctt tctttttgcc
     5881 agtatatcgc gtaatgaagt ttttttaaag aaaaaatgaa ctagtcaaga acagcagctg
     5941 ctgtttttgg ctagttcgcg tgtatttgtt aatcaatcaa tgtttgtgct gctgtgataa
     6001 tcgataattt atatacatct tcttcatttg ctccacgaga caaatcagaa accggtttgt
     6061 tcaagccttg tagaattggt ccgattgctt caaagttacc gaaacgctga gcaattttat
     6121 agccgatatt tcctgactgc aattctggaa atacaaagac tgttgcttga ccagctactt
     6181 ctgaatctgg cgctttcagt tgtgcaactg aaggaacata agaagcatca aattgcaatt
     6241 cgccgtcgat catgtattgt ggcgctagtt ttttagcgat tttagttgct tcaactactt
     6301 tgtctacttc tggcgatttc gctgagcctt ttgtcgagaa gctcatcatt gctacttttg
     6361 gttcgatatc gaacaattct gctgtttttg cagaatctac ggcgatttca gccaattctt
     6421 gcgcagttgg attcacgttg atcgcacaat ccgaaaagac atatttttct tgatctcttc
     6481 cacgtaccat gatcatcgct ccgcttgtcc gactgatgcc aggctttgtt ttgatgatct
     6541 gaagtgctgg gcgaacagtg tcgccagtcg aatggatcgc tccagagacc attccgtctg
     6601 caatccccat atacgtaagc atcgtaccaa agtaattcac atcttttaaa atcttttctg
     6661 cttgttcttt ggatgccttt ccatttctac gttcaacaaa agtatccacc atttcatccc
     6721 acttatcgta attatcaggg ctgatgatcg taaagctaga tgttttgatt cctctggcat
     6781 gcgctgcatt tcttatttct tcttcgttcc ctactaaaat gggttccaca agttcttctg
     6841 ctttcaaccg ggcagctgcg cctaaaatac gagcatcagt agcttccggg aaaacgattt
     6901 tgatcccacg gcgaacaatt ttaaatctta aactatcaaa caattccaca agatgacctc
     6961 cagacgtttt tatatgtatt atcatacact attccagaaa aaaagaacag tttattttat
     7021 ctgtatcagt caaaaatgct ggaaaaatta agtttttcac atacaaagcg aactgcacta
     7081 taacaggaaa gcgctgtttt attacactgt ttcaggcagt tgccaatcaa tcggttcttc
     7141 tcctaacgca aggagcgcat cattcgtttt cgagaagggt ttggaaccaa aaaagccacg
     7201 acttgcagat aacggactgg gatgtggaga tgttatgatg atatggcgac tcttatcgat
     7261 cattttgatt ttttcttgtg ccggttttcc ccacaagata aacacgattg gtttttcccg
     7321 ttcatttaat ttttcaatga tcgtgtctgt taatctttcc caacctttcc cgcgatgaga
     7381 atacgcttgc ccttcccgaa cggtaagaac ggtgttgagt aaaaggactc cctgtttagc
     7441 ccatgacact agatttccat gttgaacagg tgcgattcca agatcatcat aaagctcttt
     7501 atagatattt cttaacgatg gcgggatctt tacgccaggt tgcacagaaa aagataagcc
     7561 atgagcttga ttttctccat gataaggatc ttgtcctaaa atcaccactt taacttcttc
     7621 ataaggagtc aattccaatg cttcatagat atgatacata tctggatgga tcttttggga
     7681 cgcatattct ttttttaaaa attctcttag atgcagataa taatcttttg aaaattctga
     7741 ggacaaaact tcttgccaac tgttatgaat gatttctttc aaaaactcca cctcctaata
     7801 aaaagaataa ctcacatttt cttcaatcac aagggacaat cttcaccctc agcacttgat
     7861 taatatggta aactaggaac aagaagcaga aaggaacgat aatcattatg atcaaattaa
     7921 ttgcatcaga tatggacggc acattattag atgcccatat gagtgtttct cctgaaaatg
     7981 ccgaggcgat acgttttgca aatgatgccg gtgttgagtt catggtcgct actggtcgaa
     8041 actatcaaga agctcgcgca gctttagatg aggtaggaat cgattgtgcg atgatcactt
     8101 tgaacggggc acaggtattt gataaagaag gaaattcatt gtttaccgtg tcgattccaa
     8161 ataatcaagc aatctctgtt ttagatattc tagatgctaa cggtatttac tatgaagtag
     8221 caaccaacga tggtctttat tctgaaaatc aagcgaaacg aattgaaagt tttgcttcca
     8281 tggtggctac ccatttacct caccttacgt ataaaatggc gattgccatg gcctcagcta
     8341 agctggagtt attgcacatc acatacgtgg acagtattcg tgctattttg gaagaaaaga
     8401 atctggaagt tttaaaaatt atttgttttc atacagaagg gccagctgta ttaggtccag
     8461 ttggaaagca gatttctctt ttaggcgatc tagccgttac ttcctctggt caaaacaata
     8521 tcgaagtgaa ccataaaaac gctcaaaaag gaattgctgt tgcccatgtc gctcacgagc
     8581 gtgggatttc tttagaagaa gtcatgacga ttggcgataa cttcaatgat gtcagcatgc
     8641 tgcaagtagc aggagtcagt ttcgcaatgg gaaatgcaga aatcgaagta aaagattatg
     8701 ccaaatatac aacggacaca aaccttgaat ctggcgtagg aaaagccata ttgcgcgcga
     8761 ttgcagaaga cttgtagttt tatctagaaa agagggatat agatgtcaga atttttcatt
     8821 caggaaaagc agcttggacg tgcaactcgg acaattgtca aaaacgaaaa aggacattcc
     8881 ctgtttttga tggttggtcg ctggggaaca aagggcgatg cattgtctct ttatgccatg
     8941 aatggcgatc tcgttgctca catcaagcag ataagcctga cattcggtac gcgttttgag
     9001 ctgtataaag agttcgaaaa ggtgggaacc atgcagaaaa tattcaattg gcccggtgac
     9061 ttttattata ttcgtagact tcactggaca gtccaaggtg atatctataa tcatcaatat
     9121 caaatccatc atttcaatca gcaaatcatg aaaatggata aagcaacact tttttcagga
     9181 gattactatg tattggatgt ctcaaatgat gaagatgctc cagtttgtat ttgtattgct
     9241 gcggttttag attactggct ctaccgtaaa gacaaaagtc gcgataagcc tgtttcttac
     9301 cgacttaact taagttaaaa agatgtacct gaaatgcgga aatacgcatt tcaggtacat
     9361 ctttttagat attcttttgc atatgatcat aaagatgacc actgatcatc cgctgaccga
     9421 caactttgaa gtcatggcgt tcgtataatt ttttagcctt tggatttttt tcatccacac
     9481 ttagaccgat gacggagcga ttagctttct tcgccaattt aggtaatgct tctaataatc
     9541 gcgaaccgat gccttgtcct ctaaaatctt ctgatacact gatagagtcc aaataccatt
     9601 cgttgggaaa cgtttcttta tcaatgaaca aacgtgtaga cgcatctagc tgatatttct
     9661 ctaagatagg taccagtggc tgatcgatga ttgattcttc ttcatctgta taaccgtaag
     9721 caactcctgc aactttaccg tctatttctt ctacgatacc tcgtgaataa ctgtaacgat
     9781 aatttggatc agtgattgct tcttctaaaa tctctaaagt cttttgttcc cctgtctgct
     9841 tcaaaaatgg cagttccata tcttttagaa tcactaagat caatggggcg atttctggag
     9901 catcttcttt tctcgcaaaa cgaatcatag tttcctctcc ttttctccat tctaacatgc
     9961 agaaaaagaa atcgcaaaat cgttatttta aaaaatcttc aatcttttca gtaatgaaat
    10021 aaccatgagt aacgattctt gccgcttcag ttcggcaata agggcagacg actggcattt
    10081 ctttatcatt atgttcgact acattattga cttcttcgta tgcaaatttt tttccgcaat
    10141 tagggcattt cattttttcc atgttatctc aacttccttt catctttact ctcttagctt
    10201 actcttatat tgtaaaaaag agaagcaata tccttcttct atgcagatat ccatacctga
    10261 cggtcctgtt ctccatctat taatcggact ttataataag gacgttcatc tttgactaga
    10321 agtgtccact ctttcacttt tgcttttttc ccgtagtagc tgactgcttc cttcatcgct
    10381 gcttgcggtt gtttgaactc ttccgttttt tctatatcaa aaagatgttc tgcggccttt
    10441 tccgtgtcat ttcgtacggt tgttttccca gtggatggat tgactgctat gacttgtcct
    10501 tcacaaaaaa actggtattc atatgttgca ttcgtaggat tctccaaatc tttttcaact
    10561 gcaaaatgaa tagcagatat gttatgttct tttgtttcgt tttttacgat tgctgctgct
    10621 tttttcaatg agatccgata ttcctggtca ctaatatcat ccatgtgaaa agcggcattg
    10681 agcagtcccg tcataccggt aatccctatt acgatgaggg cagcaacacc aaacatcatt
    10741 tttttcattc ttcattctcc tgtttctttt ttttaaagaa tatcaagaaa tgaacgaatg
    10801 aaaaatcgaa tgaaacgaaa aaaaagtgac aattttgtta tctttattcg tgcagttcta
    10861 atggcaatcc gtctggatca aagaaaaacg tcattttttt tcctgtaaag gtatcttctc
    10921 taatcggttc tgattcgatc cctaaggact gcaattctgc aattgtttct tcaatatcgt
    10981 ctactttgaa agcaagatga cgcaagccac aagcttctgg atagtttggt cgttgtggcg
    11041 cattagcaat tccaaaaatc tccaattcag cacttcctag tttcagatct aatttatagt
    11101 catctcgttc tttgcggtaa ttttctctga ttacttcaaa tcctaattta ttaacataaa
    11161 aatcacgtga tttctgataa tctgaaacaa tgatggctat atgatggatc gttgtaaggt
    11221 tcatatgagc actcctattc ttcgtttaag ctcggatcta tattaatctc cagttccatt
    11281 gtaaatgtgt cagattgaaa aaaatagctg tttttccttt ttataagaaa aaaggcaaaa
    11341 aaaacagaaa gtaggtcctc caagacactc ttcctgttcc caatataata aatgaaagta
    11401 agaaattata ttttcaagaa aacaaacaga aaattatcat aacacaaatt atttataatt
    11461 gaaagactag ttttttttca agaaaagcat acaatagatt gtgttagtaa atacagaaca
    11521 cactacattt agtgtcttaa ggagtcctca tacatgaagt catattttca gtttttagct
    11581 catcagctaa aaggctggcc gcaacaaaat tattatcttt tctttttcag tcttggctgc
    11641 caggtcatga ctcttgtcaa tagtcccatc acttggctgt ccgttattac atttatcggt
    11701 accacgcttg gtgtattgtg tattttatcc atcaatgcgg caaaatcagt caacggtatt
    11761 ttaggtattt tgtctgcgat ttgttttatt atcgttgggt tttcagcaaa gaactattta
    11821 agtatcggtg aacagattgc ttatatgatc acattggata tccccgtatt gttaagtaaa
    11881 gaatggaatc ataatatggc ttcaaaaatc cgaaaattca ctggaaaaag ctggtttatc
    11941 gcaatcatca gtactttcct tgtctatctt gtttcaggat tcctgattgg tcgttataca
    12001 gatgatcctc ggccgtgggt agatgccatt gctttttcta ttagcttgac tgctggtatc
    12061 atcagtttct tgcgttacaa caaccaatat tactggtggt tagcttcagg gcttgctcaa
    12121 atggtccttt ggtatatttc atttacgcat ggatctgcaa gtttggcgat gttcgtgaac
    12181 agctctgttt acttgctgaa tgatgtcctt gcatttaccg tttcaccttg gtataaccaa
    12241 aaagaacgtg aacgtatgca aaaaatggaa gaagaatact acaacgcaca acaacacgca
    12301 taaaaaaact gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    12361 ataaacggcg gaatcagaag caactccttc ggaaataagc tgaaattcac aaaaatttga
    12421 aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc
    12481 tattttttat agtaattcct tcagatactt tcttgcctca tcattgaaac gcagtgattc
    12541 ttctaacttc tgataatatt ccaacgcttg tttctgagct tcagtaaaac gataatgttt
    12601 tcctttccat ttgtcgattg tctcttgcac atttccagat tggatatctt tcactactag
    12661 ttgccatttt tcttttgaga ctgtacggtc tttcttatca gcagattgta tagcttctcg
    12721 ttctttacgc aatactttta aaaaaccatt tctgactatc cgaggaatgc tgtcgaagaa
    12781 cccttctgta aaaccatagc gacaatattg cttccaaata aatagataga tttttggttg
    12841 gatgaatcgt tggtttttcg caaaatattc catctgagca gtgacttctg gtgttcgttg
    12901 acgttttgtc tttctcaaca cggaaataaa tgtttcaaaa taagcattag gatgtactgg
    12961 cacttcttca tatacgatgg attgccaatc tttacgtaaa aatgcgcgat agttgatcaa
    13021 gaattcttct acatccttta ctaattcttc tgcaaagtcg tcaaatagtt ctttttttat
    13081 ctgtcgcttt tttgactgcc cattgatgtg ttcacttatt ttcttgattt cattttccaa
    13141 tgcttgataa tccgctatgt aaatcggcat ttctacttgg cgaaattctg cgagccgctg
    13201 agctaatttt atatccggta aatatggctg tttcatccga cgattttggt acaagacaaa
    13261 acagtatttt tgccatagtc cttcaggaaa atcgatattc ttctgcttaa gccataataa
    13321 ttcgtctaag gccagatcaa cttttgtcat tccctgatgg atcgaagctg ccactgtcgg
    13381 agagacatgc aggtgatcgg caaagtggtt gatgcctagt tttaggatct ttccggtttt
    13441 tggcgattta acgatgtatt gcacttttaa ttctctacca cactcacaat acaatttttg
    13501 ctggcttcgt tttgttcgaa aaaacggatt gatcttgaag tcagaaaaca cccaatctgt
    13561 tgcagctaaa taatgttctt ccataaaacg agagctgatt agatatcggc ggtgcttatc
    13621 gactagttcc cgctgttttt tcgttaattt tgcatagatc tcccaacgtt gactgcttgt
    13681 gtattgttta gaaattttga tgggatagct tttgctgatt tcttttggaa accattcttc
    13741 aaagacggat tgccattctt ccacggctgt ttctttcctt tctaattaca aaaagctggc
    13801 tactaacata acgttagcaa ccagccagtg tttcttgcta aattagtgag ttgttccggc
    13861 atttccggaa atatcttctt tattttctac tgctgcttca actgcataag tcaaagaaga
    13921 agcaatgtct tctaaagaca tagaaggcgt acccactgga cgatcgatca cttgatcggg
    13981 tgagaatggc acgtggatga acccacctcg aattgccggg aattgtgttt cgatcaaatg
    14041 aagcaaacga tacatgatat cattgcagac aaatgttcct gctgtataag agatgtacgc
    14101 tggtagacca tgatcttgta cattttttgt cattgctttg attggcaatg aggtaaagta
    14161 agcggctgga ccgttttctt ctaaagccgt atcgaatggc tgatttcctt cattatcagg
    14221 gattcttgct tctgctagat tgattgcgac acgttcgaac gagactgctg accgcccacc
    14281 tgcttgtccg acacagatca cgacatctgg ttggtgctgt tggatggctt ctttgactac
    14341 ttgactgctt ttttcgaata cggtcggtac ttccactttg atgatttcag caccgctgat
    14401 ttcatctggc atttttttta ctgcttcata ggctggattc actttgtccc caccaaatgg
    14461 atcaaaacct gttactagta ctttcatctg ttgttcctcc gtccgattga tttaacatat
    14521 ggttatcttt tatcatacca ttttccttga acagcgtaaa taaaggaaac aatagtttaa
    14581 ataacataac agatcaaact taatatattc ttataaaaaa atagtgttcc actacaatgt
    14641 tagaagaggt tgcgacaatt attctgtcac aacccctctc ttctattatt taaatagaat
    14701 catataagca atttggaaaa ctagcataaa taacgcaata aacaactggt ttttgatcac
    14761 tccgtatttg tctttcatct ccagcatcgc tacaggcacg atattgaagt tagcagccat
    14821 cggtgtcagc agggtcccac aaaaaccgca agtcaatgca accattccta ttaacgctgg
    14881 attcgctcca tgactaaata cgaatggtgc accgatccca accgtcatca cggtgatggc
    14941 cgcaaatgca ttccccatga ttaccgtaaa tatcaccatg ccaagagcgt aaatgatgat
    15001 gccgatgttc acatttcctt ctggaacgat cgtacctact gcattggaaa tcactgtccc
    15061 aactccagcg cttgtaaata ccgcacctag agaagctaaa agcattggca acatgcttaa
    15121 cggccctact gtgcccagca tatctgctgc atcatctaaa aatgtacttg gtcgattatc
    15181 tcgagaaaag aacatcaaga ttagcatggc tgcaaaaaca ccgacgccca cgccgactaa
    15241 agcacctaaa ttggtaaata gagcaaacaa gatcgcaaag acaccgatac tcaaagctgg
    15301 aataaaaatt ttcattccta gtttgtctga cattttttcc atatattctt ttgatggaag
    15361 ccggctttct cctttgctga ctttcttagc aattgctggt atggtcatcg caaagatcaa
    15421 gccaccgttc acgattgccg gaagccatct tcctcctgca ataactaccc ctaagaccgt
    15481 ccagaaaaag gctgacccat aacgatgagg attggtttta tctgttaaat ttcttactcc
    15541 agtgtaaatc aatacgagtc ccatcacgat ataaaggatc tccagtaatt tatcgcctaa
    15601 taaaatatca ggatttgtaa aaaagtccat cttattgccc ctttcctgga taatatttct
    15661 tcgctaattt cttttcttta agatagaagt agacacttgc cacaactaaa gccgtgatag
    15721 ccactggaac ttccactaaa gcaagctgtc ccaatgttac gtcatatcct agatctttca
    15781 gtgttgattg gactagcaac gcccctgctc ctccgatgaa tagtacttgg ccaaagaacc
    15841 agcagacatt ttccatagct gaagccatac cttttagttc ttcttcatgc ttaggatgga
    15901 cttctaaccc ttttgattcg atggtcccca atgccatcgg taaaatgatt ggacgaacaa
    15961 atccagcaac tccaccaaaa ctcacgttaa aagccgcaaa gatcattcga aagatcccat
    16021 aaatatctac aatcatacca gcagatactt tcttgaatcg cttgatcaag gaagccgctg
    16081 attctttcag accatttctt tcaagcgttc ctgttgccaa cataatgatg ataaaaatcg
    16141 ccatcccgcg attgtttaca aaacttgtac ctaaaagttc taacattcct tgaatgccaa
    16201 gaccgccagt caaagctgta acaactaaag caaccattac gatcaatatc gagtctaatt
    16261 taaatgcaaa cccgagaata acaatcaaga cgcctaaaag gctaag
//
LOCUS       DADXTG010000056        16168 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.057, whole genome shotgun sequence.
ACCESSION   DADXTG010000056 DADXTG010000000
VERSION     DADXTG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.057"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(203..1513)
                     /locus_tag="IWI57_001538"
     CDS             complement(203..1513)
                     /locus_tag="IWI57_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBC2449686.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1525..2943)
                     /locus_tag="IWI57_001539"
     CDS             complement(1525..2943)
                     /locus_tag="IWI57_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2449687.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3183..4751)
                     /locus_tag="IWI57_001540"
     CDS             complement(3183..4751)
                     /locus_tag="IWI57_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBC2449688.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4755..5165)
                     /locus_tag="IWI57_001541"
     CDS             complement(4755..5165)
                     /locus_tag="IWI57_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBC2449689.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5162..6085)
                     /locus_tag="IWI57_001542"
     CDS             complement(5162..6085)
                     /locus_tag="IWI57_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBC2449690.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6082..6960)
                     /locus_tag="IWI57_001543"
     CDS             complement(6082..6960)
                     /locus_tag="IWI57_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBC2449691.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6947..8284)
                     /locus_tag="IWI57_001544"
     CDS             complement(6947..8284)
                     /locus_tag="IWI57_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBC2449692.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8287..9390)
                     /locus_tag="IWI57_001545"
     CDS             complement(8287..9390)
                     /locus_tag="IWI57_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBC2449693.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9464..10972)
                     /locus_tag="IWI57_001546"
     CDS             complement(9464..10972)
                     /locus_tag="IWI57_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBC2449694.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10938..11630)
                     /locus_tag="IWI57_001547"
     CDS             complement(10938..11630)
                     /locus_tag="IWI57_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBC2449695.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11652..12626)
                     /locus_tag="IWI57_001548"
     CDS             complement(11652..12626)
                     /locus_tag="IWI57_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2449696.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12926..13798
                     /locus_tag="IWI57_001549"
     CDS             12926..13798
                     /locus_tag="IWI57_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2449697.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14256..16136)
                     /locus_tag="IWI57_001550"
     CDS             complement(14256..16136)
                     /locus_tag="IWI57_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449698.1"
                     /translation="MDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNL
                     FWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFT
                     TQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMI
                     RNLRIGLVAVSLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQS
                     VSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYT
                     ALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDA
                     EASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGP
                     SSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNP
                     LSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLP
                     ITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGE
                     KFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKK
                     YL"
BASE COUNT         4762 a         3406 c         2633 g         5367 t
ORIGIN      
        1 aagttttttt cgatagaatc aagcttcgca agtggttctt tttaacgact tagttatctt
       61 atcacgtcta aaaacatgtg tcaactcttt tttggaattt tcataaaatg tttcaatgaa
      121 gcgcgacaac gttgtttaat atatcattaa aaaaaataaa ggtcaagcta aaatattatt
      181 taagcttgac cttattataa tttcaacgat tgtcaaaagt attgtttgca gctgtgttgc
      241 cagtttctat agataaatca cagcttttga aacataagtt ttgtactgct tcgattcgcc
      301 cgaattcttt tgcagttatt tggcaatgag aaaagcagac atcttctata ggcttttcag
      361 gaaatgctac taaatcaaaa gctcgggaag gaagtgagta acctggagaa aggcatgcct
      421 gtacgttttt gatgtgtatg tctttgactt tcgtcatttg ctttgattca ggtatttctt
      481 cagccactgc ttgccaactt tctggcattt gagcaaggtc attgctattt tttctattgt
      541 attcattatg ccagtccatt atccaagaaa aaggaaattg tacatcaatc atattcagat
      601 tttctaccct gatattttcg atcactcccc ctctttcttt tgaagatttc attcgaatac
      661 cacagtctga ctgaaaaaaa tcgatatcgt gaatatatac gtcactaact ccagcactaa
      721 cttcgctacc aatagttaca ccgtatcctg aacggatttt acatctagca acctcgattc
      781 ttgatgattt tcgattgact cttgcaccat tgccatctct gccagactta atagcaatac
      841 aatcatcacc acaggccaaa tcacattcat agacacgcac atttgttgaa gaatcaatat
      901 caatcccatc tgtacttggt ccatcattgt tgcggatgat tactttttct actaatacat
      961 cgttagaata agtgatttgc aagttccaaa aacctgattt ctgaaaaatc aaatcacgaa
     1021 caacaacatg ttctgcatga tacagtaaac aagcgcgtgg acgtttaata gcataatccg
     1081 caatccaacg aagtcccttt tgatcgtatt cagctcttgt cccgcttttt tgatcttttc
     1141 cccaatacag ctcccaccaa tggtctcctt gaccgtcgat tattcctttt ccttctataa
     1201 gaatatcttt cgccgagaga acattcaaaa tcgctgctgg ccatttcatt tctacgccgg
     1261 ctactcgtgt atcgatttct ggaaatgctt cgatatctgt tctccccttg aggactgcac
     1321 cttcttcaaa gataagatga ctcttatctt ttaaaaaaag ggctccgact aaataaatgc
     1381 cttttggaat aaatacaggt aagtgttctt tcgcaccttg atcgattgct ttttgcagta
     1441 ttattgtatc atcttgttga gtgtcgtttg ttttaaagtt caaagcattg ataatccctt
     1501 tttttgtccg caatacgttt ctccctatct ttcattgatt tttttcaatt cttcaccaat
     1561 aaatacagtt tttgaacgat tcagtgggta acctactagc aaaatgattg cggatacaaa
     1621 taagcagaat gctggtaaca agtttgccat cgtataaatc ctttcttgta ctaaaactga
     1681 ttggatggct ccacctgttg tagacgattg gtaaccaatg agtgccagca taaatccgcc
     1741 taccgctcct gcacatgctt gtcctacttt tctcgcaaaa gaatttactc catatactgt
     1801 tccatcttct cttaagcctg tcttatattg atgataatca atcacgtcag taatgaatgc
     1861 ccagaccatc aaattgaata aaccagatcc taaagtagcg ataaataaaa gaatcagata
     1921 tacccatgca tttgttatat gaaggaaata catcagcaag tacatacatg aagaagcaag
     1981 taacgcaaat acgcttgctt cctttttacc aaataatttt gtcagctttg tagcaaatgg
     2041 tgctaaaact actactgttc cgtaagtaaa taaaagagca acagacattg cttgtttatt
     2101 atggaagtaa tcattgaata aataagtcat attcgtccca gctaaaatct gattgatcac
     2161 aatgaaaatg tcaacaatga ctaaaataat cagtgctcga ttagctaaca aaccttttac
     2221 caactgagaa gcagatactt tttcgttttt gacaactctt actctttcag tagtcaattt
     2281 acaagttaac atatatgcga caaatgctat tgcagcacat ccgattgaaa tccaaaagaa
     2341 atgattccct gaaacgactt gttgtccact cttgttatgg gtatacatta aaatcggaat
     2401 gaagaatcct gtagttgatc cgccaatcgc agaaccaatg cttcggaacg tggacaacga
     2461 ggtgcggtca ttaggatttg agctaattgc tgctgccatt gctccatacg ggatattgat
     2521 tgttgagaga aaaatcccat aaacaatgta tgaaacaaaa atataagcaa tcttcaccgt
     2581 cattggaaga tgttggacga atggcaaaaa caaaatgaca gtcactaagc aaaatggata
     2641 cttcattttt ctgatccaag gaataaatcg gccagtttct gttaactctg agacatcaca
     2701 taatcttcca accatcacat cagcaaacgc atccaaaatt ctcgaaatca agaataacgt
     2761 acctacgatt gcccctgaaa cgccaagaac attcgtataa taaatcatca agaaactatt
     2821 gactaaacct aaaataaaac agttacctag atcaccgaac atgtagccta tcttatcgga
     2881 aatcccaaat tccttttttt ctttttttat tgtatttgct gtaacagcat tttgtgaaag
     2941 catttgaatt cctcctacct cttctagtaa acgttttcaa aattataact ctttttttct
     3001 tgtttttata ttcacaatgt tggcttttta ttgcattatc ttgacagata aaaaacaaaa
     3061 aataatctaa aaacattgat gcataagatt gataactttt tttacataac aatatataca
     3121 aacaaaaaaa ttcactaact agtgaaaata agttagtgaa cctcatttat taaagaatta
     3181 aattacagat gatcttttcc atcatacttg aaaaactcaa aatcagcata acttcctctt
     3241 tttttattta gatcatgcac tgctatcccg ataaaatttc cagtaaaccc tcctgagagt
     3301 atacacatgt ttttttgcgg acctactttc ttccattctt cagttgatac gtctttcaaa
     3361 tgaaactgtg ctgtctcttt ctttacagtg actttcaaaa aagtcttctt ttctgtgagt
     3421 ggaatcgtcc aatcttctat ttgtgctact ccgttttctg attctatcat acgtaggcat
     3481 tttcccttgt cctcattcca agtaacataa caataaatat aattggattc atttaagtac
     3541 agtaccagtc cagccatctg attaaaatta ttaggcatgt attcaaatgc tgtctccgca
     3601 tcaaaagtaa aatccgtttg ccgaatagct aacaaatgat gttcgaaggt agattgaaga
     3661 gattcaccgg aaatcagacg taaaaaacct ggtctacatt ttgtatcaca ccaatcagca
     3721 tttggcaaaa tgcgtctggc attccagttc ttctttagct ctccttcaaa tgtatccata
     3781 aactgatgat ctttttcttg ttgtacctct tcatttgtct caatcattat ttcttccatc
     3841 ggagttgtct ggctatcttt aagtcttaac caatcgtcgg acgtccaata cacttcttga
     3901 atagcggttt ctcttcccaa aatagccgca ccgtttaatg gtctggtaca aagatatgtc
     3961 atgtaccatc ttcctgacgg ggtttgtact aagctcccat gtcctgtaca ttgcaatgta
     4021 gaagcaggtt tgtcactagc tgtcagcata ggaaaaccag gatcaagctc gtaaggacca
     4081 gtaatattct ttgctcggca aactgtaact gaatgtcctc ttcctgtacc gccttctgcc
     4141 gtcatcaaat aataataccc gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat
     4201 tctgtacctg caaatatttt atagatggga ccatcaagct tcttcttctc tgcatcatat
     4261 tcttgtaata cgataccgct ggatttattc ggtgtgtcca gacgataatc ccatagttca
     4321 ttgacaagcc atttttttcc cgtttcaaga tcatggaaca aagaaggatc aaagccgtta
     4381 ctatttaaat aaattggttc ggaccaagga cctgtgatct cagatgcagt gatcaaataa
     4441 ttgtggacat ctttaaacgg acggcctgca cttttgacat ctgtgtaaac aagataaaac
     4501 aatccatcag cataactgat ctgtggcgcc catatacttc catttgtagg atttccttgc
     4561 aaagatactt gagtggtcaa aatatccgtt gtatgtgacc agttgactaa atttttcgac
     4621 tcatatactc ggattcctgg aagccattca aaagaggaga caataatgta ataccgctct
     4681 ttcactctta atatattagg atctggattg aatcctctta aaataggatt tttcactttg
     4741 atagtctgca tgattcaggc tccttttctc attcttactc gatataaact gaatgcccca
     4801 gctgcgaaat aaaaaaatgc aaaaggaaac gcaataaact gagtgccaat caacaatatg
     4861 aatcctgtta taaaatacaa accagtggca gttgttaagt attttccttt agcagtcaaa
     4921 aacaatgcta ctgctaaaaa agcaagtgaa ataaacaaca caacggcaaa cagaccagtc
     4981 atgtcctgta ataatccaaa tgtattttct aaagacaaag ttgaatgatc tacagcaagc
     5041 gactgataac tttttttaaa ctctgacgca gacatattac ttaaagtaac tgctaacccg
     5101 ccaaagaaaa tcaaagatag actgccaccg accattccta gcaatagttc ccattttctt
     5161 ttcatttctg aaccccctgc ttttctaaat gtatccattc aggtcttttg atcgatgttt
     5221 cataattgtt attacgttca gagaatttga cagtctgttc atttttcttg tcatcccaat
     5281 tatcccaacc agttgggtga acatgcccac ctatcttaca cgcttcaaat aagacatttg
     5341 catacggacg ccacggtctt cctaaataga cgtttctagt atcaggttct gcagtcaagg
     5401 tacagctttc aaaacgatac cctgtttttt gatttttcgg cgtagatgct gctgtgatgt
     5461 atccaggatt tttactggct aagctatgga tatgacactg atgaaaaacg gcatctgccc
     5521 caccaaatat gaaatccacc gttccttcaa tccagcaata ttcataggtc tgacgacaat
     5581 actcaggatt ggcagaagcc ggtgtacaaa aaggagtacc atctttttgc aaatcaggca
     5641 acggacccgt gcataaggta tcctgataac cagatagtcg acagtttttt aaagatacgc
     5701 gatctcctga attgaataaa gcaacagcct gtcctacctt ttctcctgga cctgctgtat
     5761 tttcgatgtg aagattttct aatacgacat tcgatccttc taagaaaaaa gtagcacttc
     5821 ggaaagtccc taactggcta ccgtcaagat actcctgttt tgcaaaacga ttaccggaga
     5881 taactactgt tcctgctccc acaagtttaa aattggataa tctgctttct atatattcat
     5941 catatcttcc ttcttttact atcaagacct ttggatggct atccggcagg cctgccaaat
     6001 aatcgattcc ttcttggata gtttcaaaat ccgtcggggt atctatagca ttctttccaa
     6061 ttatcagttt gtctttcatc ctcataaatg ctcccttatg aattgtttcc aaatatttct
     6121 catttcttgt gtttccatat gaccgcctgt taaagaaaaa gaagaaaagt tttctggact
     6181 acccattgat tgatactttt tgtccagccc ttttctcagc ttcagtacgc cttcgtccaa
     6241 gcacatcgta tcgttttttc caactaagct aagacgaggg cgcggagcga ttttttcttg
     6301 gattgctaac gttgtataag ctgtcaatag atttggcaca tagtaataaa acccatgatg
     6361 atctaggcgg cgatgtagca aaagtatttc gatgtccacc tgtgccgcta gatcaataca
     6421 aactttgaca cgagaatcca atgctgtcac ccaccagctt aataagcctc ccatagaaag
     6481 accaatcgtt gcgagccgtt gagtatctgt gtcttccctt gtctccaaat aatcgatcaa
     6541 gctgatcaca tcataaatac gcattcccca taacgttttt cctgttaata aaaattgttt
     6601 gaacaactcg ctttcagaaa tacctccgcg ttcttcaaat ccccaagcat caatcgacca
     6661 aacagcatat cccattcctg tcagtacttc tgcaaaagat ggagaagcta agtaaggagc
     6721 accatggatc aattcacttt tcccgactga aaaatctccg ccgtgagaat gcaggtaaag
     6781 gaccggcgga aacggtcctt ctttttctgg ataagcatag agggctggta ctgtttccaa
     6841 ttcatttaag tacaattgat agtattcaag cataaaaccg ttttgttttt ctcggtataa
     6901 tcgttttcca ctaggggtat gtctgctaga tggagcacct aaaaaatcat tcaactgcat
     6961 aagcaaagtt tttacgaaca accagttcat ccatattttc tgattgattt gattggaatt
     7021 tctcaatact gatttcttta gtattttcta atagaaatgc tggtccctcg tgattttcaa
     7081 tcgacatctg gttaaaggaa accttacccg caaagccaat ataaaatcct tgtctgctca
     7141 tttcctctat tcctgtcatc atagcaggaa caccgggaac tgcatcttgg gccatcgaga
     7201 catcgatatt gtcaaatgtg atttcttgta tgaattgttc cgctaatcca taaataaaac
     7261 ctgcagaagc atggacattc cttgccgtga tattcgaaaa atggatcctt ctgaatgctg
     7321 gtgtccgttc atctatcgga taagcctttt tttcccatac atacggttct ttccctcgcg
     7381 gaccacagaa ataataaaga ttcagtataa atggacacat cacattatcc atgactatat
     7441 tggatactcg aatatcttca acgatcccgc ctcgacctcg tctagatttc aatcgtattc
     7501 ctcgatcagt ttcttggaag atacaatttg aaatggtaat attacgaata ctaccactca
     7561 tttcacttcc taatacgaca ccgccatgtc catggaccat cgtgcaattt gtgatcgtga
     7621 tattttcgca agcgatcctt tcgtacgtat cttcagtccc tgccttgatt gcaatacagt
     7681 catctcccac gtcaatatgg caattactaa tacgcacatt tttgcatgat tctggatcaa
     7741 ttccgtctgt atttggtgaa tccgctgggt tcaagatcgt caagttatca aacgttgcat
     7801 tactgcaaag aatcgggttg atcgtccaac ttggtgactg gatcaattta atatctttca
     7861 cagtaattcg atgacagtta tggaaactca tcaatttagg acgaggataa gcgagattat
     7921 ctggttcatt tctaaaagta tgccaccatt tttttccatt tccatctaat gtaccgaaac
     7981 cagtgacaga gatgttctct acattttgag catagataca ggaagcataa acttttcgat
     8041 ggactccttc ccaacgtgaa tgaacaacag gataatcttt tggatcgtca gagaatttca
     8101 gaaccgctcc tgcgcttaaa tgaagttcga cattgctttt taaaaataaa gctcctgtta
     8161 gaaattcacc tgctggaaca actacggtcc cgcctccgtc acttgctgca gcatcaatgg
     8221 cttgttgtat tgcttcagta ttcaattcat caattgatgc gccaaatgtc aaaatatcat
     8281 acatatttat aatgcctcca cttcaatcaa tgcttgcaag aatgcaccaa cgcctttttg
     8341 atcattacaa ataattggtt cactgatgta gtaagcatat gaaccatctc gctgatctgc
     8401 tccgcctaac cctgctactt gacagttttt attcaagttc agccagcctt cttttgtttc
     8461 cagaacaaat tcatcaagta atccttgatg tgatttcttc acttgtggaa gccattcttc
     8521 tttgttcagc agtcccaaac gtattccttt agccattgcg taaacaaaca tactactgcc
     8581 ggaagcttcc agataatttc ctttccgttt tgtttctgtt gttacttgat accatgtccc
     8641 gctgttttct tcttgatatg gaagtaatgc agccagtgtc ttttctagaa tttctttcag
     8701 caaagaacaa tctgtttctt catgaagtaa ttcataggta tctgctgcag ccatcaaata
     8761 ccagccaatc gatcggctcc aaaaatgatg ggatagacct gtttttggat cagcccatgg
     8821 ttggaccttc ttttcatccc atgcatgaat cagcaaattt gtttttggat cgaatagatg
     8881 tttataactg atttcaaact gtctcaaaac atcttggtaa tctttttctt cactaaaatt
     8941 ctggataaat tcagcataaa aaggagaacc catatataag ccatccaacc aaatttgata
     9001 aggataaatt tctttatgcc aaaaagctcc ttcggatgtg cgtggatggt ttgccaactg
     9061 ctgggaaagg agttctgctg cttttttata tttttcttcg cctgtttctt tgtacaataa
     9121 aaagaaaagc tttcctgtat ttacatggtc gatattgtat tcttcaacgc tgtagccacg
     9181 gatcgttcca tcttcttgca cgaaatagtc gatattgtct tttatgaaat caaaataaat
     9241 tttttcttgg gttcgttccc aaagtaattg gaacccttta agtacgacgc catagtcata
     9301 agaccatttt cctttatacc acttttcttc atataagcga ggtgttcttt ccatgatact
     9361 ttttgctaat ttctttgacc agttttccat tatttgctct cctttaaaaa ttaaaatagg
     9421 atttgggaca aataccaaga agtatttgtc ccaatcgtct atattactgt gccgctgctt
     9481 tgtatgcttc ctcgtactct tgtgtgattc gatcgcctcc ttgtgctttc cagtcttcta
     9541 cagctttttt gaatccatct tcgtcaagct gtcccatgat gtattgcacc gtagcatcaa
     9601 tgacgatttt ttcaatttct gatcctactt cattgttcgt cggtgaatcc aatggcacag
     9661 aaggatccat tacagcaaat tcttcatttt ctttgatcaa ttgattagag aattcttttt
     9721 cagggtctgt atctttgaaa gagaacgtca cttcactagg acgagagctt gataacggtt
     9781 gcacctcttg ttgccaaaga tccatatcga taaattcgat agaaccatca tcattcaatt
     9841 cataatgcgt tccttcgata ccgccagtca tcaatttata cacatcttct tctaaaagat
     9901 catcaacaaa ttgaagtaat ttcttcaatt gtgcttcgtc ttttacttga gatttgggaa
     9961 atgctagcaa gccgccaata ccatttcctt ctgaccaaac atgataaccg ccgccttcac
    10021 catctgagat cttgtttaca ggcactaatt ccatatcatc ttgaatacct tgacccatcg
    10081 tcttcagatt cttgatatcc accattccag tgtagattcc agttcttcct tgtgcaaatt
    10141 gttcttgctg atcagtcttt tgggtaactg caaagtcttg tgccaaatag ccattctcaa
    10201 atagatctct tgaaaaattc aaagctttca tatactcttc tgtatcaaat tctggagtga
    10261 atttgccatt atcgtctaca gcccagccat ttggtgctcc aaaataagaa gtgaaaagtt
    10321 taaagctggt ataacgaata tcactaggcg cacgatcgcc aaaaccaact gtgtcgtcca
    10381 caccatttcc atcaggatcg ttttctgtaa attgtttagc tacttcataa agctcatcca
    10441 tggtagttgg aacatctagt cctagattat ccaaccagtc ttttcgaatg actaagccag
    10501 cacgagcgta gtctttttgt atcggtacgc cgtacaaggc accgtcgata ctcgcagctt
    10561 cgattctatc ttcagaaatt tgacttaaat ttttatagtc ttttaaatat tctgatacat
    10621 cccagaatac gcctgatttt aacgcattgc ggacagaaga gtttttcaac atcgcaattt
    10681 gaagtgtaac gatatctcct agttcatcag aagccaatgc agttgttaat ctttcttctt
    10741 ttgaagcatc aggaatccag ttgaatgtca gttccgtatc ggttttttct tcgatcaagt
    10801 tcacaatcgt gtctgtaggc ggtgaagcat gatgcaaaat atttaaccaa gtgattttag
    10861 cttttccatc ttctgtctct gaagaactgt tacttgaact gctgccgcat gcggcaagtg
    10921 ctagaattcc tgcaaaacta agacctaatt ttacgagcgt tgcttttttc ataaattttc
    10981 tccttcactg ttttaataat atttttgttt tcttctgttt tttctgtata tgagttcttc
    11041 cattccaaat ggcaaaatat agaatttgct gtccacgtga ctgcggaaaa gaagagactg
    11101 ccaccgataa aggttaaaac acctggcatt gtttggatga tcatcagata aatccctacc
    11161 atacttaata tcaacactaa tgagtaatga agatgtgaga atccgaataa aaatgccatt
    11221 ttcattttga aaaaatatcc tttccaatca taatgagcca tgattgggaa aatatagagt
    11281 gctgagagca catatatgaa cgcagcaata tacaaagcga tcctaagata ccaattgtgt
    11341 atgaaagaaa gatccacaag taaaacaatc ccgatggcgc catatatcca gctcatcagc
    11401 aaggtttcac gatagttttc tttaaaatat gtgaagtacg ttttcgtcaa aggaaacgtt
    11461 tcttttgagc gcagccattt tctaatcact gcaaccatcg catacgatgc aggaccgaaa
    11521 gtaaaaactc ccaaacctgc gatgactaaa ccgccccaca gaaaattcaa ataaaccaac
    11581 tgtaaaatac gatttagcct tccgtttatt ttatctaatg actgcagcat atctttccct
    11641 ctctttctat cttagtaaac gccgtcttct cctagctttt tggcaatctt attagctaaa
    11701 attaccataa acaatcctac gaccccttta aacaatccca ttgctgtact gaaacttaac
    11761 tgtccattct tcaatccagc tgtataaata taggtgtcga aaatttctgc aacgcttcga
    11821 ttcaatgaat tcaataacaa gtacatatgc tcgaacccta agtctaatgt ctgaccgatt
    11881 tttagaatca gcaaggtaat gattactgga cgaattgccg gcaatgtaac atgccatgtt
    11941 ttgcgtaagc gtcctgctcc atccatttct gcagcttcat acaactgtgt atcgactgca
    12001 gttatcgctg aaaggtagat gatcgtagac cagcccaact ctttccagat cacttgtccg
    12061 atatatactg tgcgtgtcca ttcaggggat gttagaaaac tgattttttc aaatcccatt
    12121 gttgcaagga attcattcaa tacccctccg tcaacattca aaaatacata agtaatcgaa
    12181 acaatgatta cccaagacat gaagtgaggg atgtaaatga tcgtttgaat cccgtttttc
    12241 aatcgtttgt tcgttacttc atttaatagt aaagacaaaa tgattggcat cggaaaaaac
    12301 acgaagatat ttaatccgaa caagatcaac gtatttctta ataatgtaaa gaatgtcggt
    12361 tctgtaaaca aacgaatgaa atgtttgaaa ccgacccaag gacttcccat tattcctaaa
    12421 aatggttgat aatcttgaaa agcaataacg agtcccccca taggaagata cctaaaaatc
    12481 aagaaataaa ggactcccgg cagaatcatc acatataaaa atttattcgt tttgattcct
    12541 gcaattattt tttttcgtct tctgagcttt tcttcttttt tcgatagcgt cagttgaaat
    12601 ggttcttgtt tctttacact ttccataagc ccctccttag ttttgtgaat gtttgctgct
    12661 tatgacttca tgatagataa ctatctgaat tacgtatata atcattttta ccgtaagtaa
    12721 aaacgcttac atagcaatga tttttgtgct atacaaaacg ataaagtgta ttttttcaca
    12781 aataaactac gcatagcaaa ccttctattt ttttctatct gacacttagc ataggaaatg
    12841 attatatacg taatctttca ccgatgataa attgaacata tagaaacaga aacatttaat
    12901 ttattttgag gaggagccta tctatatgaa aaagaaagtc tctataggag aaagaagttt
    12961 tacacttttc aacaatacgt ttctaatttt gctagctttg atctgtatcg ttcctttttt
    13021 aaatatcatc gctacatctt ttgcttctac acaagaagtc gtcgcgaaaa agtttatcct
    13081 ttttcctact accttttctt tagacgcgta tcgctacatt ctttctacgc cgaccatttt
    13141 tagagcactt gctgtttcga ttggtgttac aggtgtcgga acgatcgtca gtatgtgtgc
    13201 aacttcactg atggcctacg gtctttccag aaagtatttg tttggtcgag gtttcgtcaa
    13261 ctttcttgtt gtattttcta tgttgttcag cggggggatg attccaacgt tcttagttgt
    13321 tcgttctcta gggctggtca attcttactg ggccatgatc ctgcctgtgg ctgttaatgc
    13381 aatgaatatg atcattatga gaaacttttt ccaagccttg ccggatagtt tggaagaatc
    13441 agcaaaaatg gatggttgca cagattttgg tgtatttttc aaaattatgc tgccattagc
    13501 gttaccgtct attgctacta tctcgctttt ttatgctgtt acttactgga acacttatat
    13561 gactgctatt ttgtatatca acgattcatc taaatggccg atacagattc ttttacgcca
    13621 aatcgtcatt gtatcaagtg ggatgcaagc agaaagtagt gccgttgatg tgattccacc
    13681 agcacaaaca gttaaaatgg ctgttatcgt tattgcaaca gtgcctatgc tgattgttta
    13741 tccttttgtt cagaaatatt ttgtcaaagg agcactcgta ggttctgtca aagggtgatt
    13801 atgtactgaa cgcaaaatgg tagttgtgtc agaagtggtc agcttcaaga aataagaagg
    13861 catttacgaa aattgcttct taaatttttg tgaaatttcg gcttattttt cgaagaatct
    13921 actcctggaa caccgtgtat cagaggttgt gaaagagacg ttcagccttg agcgatgata
    13981 aaaactctca caaacattgc gacgagtaac ccacaggagc aataagaagc gattttgaaa
    14041 aatagcttcc catatttcac aaaattatgc cttattgtcg atagcctgtc tcttgaacgc
    14101 catttattcg taaaaaagaa ctgtgacaag acttttgtca cagttctttt tgcgtgctta
    14161 gccttgaaat ttaactatcc ataaatacaa atactagggt tattctaact tcttttattg
    14221 cttgaatcag tccacaagaa aaacagcctc ttgattcaca aatatttttt tcgatattct
    14281 cccggcgtaa tcgattcctt ctttttaaag aaacgaataa aattttgcgg atttcgatat
    14341 tgcaatcttt ctgcaatctc ttttacggag aggttagtct ctttcaacca tttttttgct
    14401 acctctaaac gatgattttg aacaaaatca ccaaattttt ctcctgtttc ctttttaaat
    14461 acattactta aatagttagg gttataatgc aacctatcag cgatcacatc taaagacaga
    14521 tcacgatcat attctgtgta gacgatatgc ataatattct ccgacaaatt tttaaattca
    14581 tgatcagtga ttgcttgaga gcattctgtg attggcagaa ctaattgttc taaaacaagt
    14641 ttttctagtt ctttgggatg ataattgttc agtgctttta cataaatttg tttcatgtta
    14701 tcaaagtttt ttgaatctac tcccaataac tgtcctaatt gaacgagttc attgatcaag
    14761 cggaccgaaa ctacttcccg actcaaagga tttttatttt gtgcaaacaa tgcatcaatc
    14821 aattcatgta ctaaattctt cacttcctga tttccagaac gaatggcttc aaacaatcga
    14881 ttttgttctt ctatcggata tctaataagt gttttttcat gagcagcagg aacgatttcc
    14941 tgataaaaaa taattgaact tggaccagta tttacacgat aatacagggc ttcttttgcc
    15001 cgatcaaccg attgcttgct ttcattcagc gtttcgaacc ggttactgat gcctatactg
    15061 atagttaaat tcaagtattc ttttacacgt ttttgaatga gtcggcaata ctccatgact
    15121 tttttgcttg cttctgcatc atctttatcc attcgataaa tagtggcttg catttcttca
    15181 ttcaaaacga tcggcagcat tcgattttcc tttggcacaa tttccgtgat catgttgttg
    15241 atcgctaaaa gtagcaaatc acgctcccca ccgtgactat cttctaacag atcgatttga
    15301 accaaagctg tataatagca gtcattttga gaactataac caaattgctg caaccgctga
    15361 tctagttctc gcctttctac tcgatttcta aataaactaa gtacaaatag cgtttccagc
    15421 tgtggctttt gggtaactaa gtgtgcactt aacgcttctt tttctcctat gatccgactg
    15481 actgattgag cgaccaaatc tagttctttc ccactgaatc cttttgattt aagattcaga
    15541 cgttcctgta tttgagaaat cggacgggca aatctgtccg aaaataagta ggaaagaaga
    15601 ccaatgagtc ctaccagtgt taaagacact gcaactagcc cgattctcag attgcggatc
    15661 attgcgccaa ttgcactaga gtcaacttct aatctgtaaa tccaattatt ataatctgat
    15721 ttcagtacta tatacgtttt atctttttct cttatcactt tcagctgatt gtaatctaga
    15781 tccacgtccg ttaatacagt tggttgctct ttatttttta ctgtctgagt agtaaaaagg
    15841 ttgccaactt cgttttcaat agaaagtatg tcttctccac tttcaccaac aacttgttct
    15901 attgtatgtt tctctatcgt agcaattccc aatgcatatt tttctttttc aaacattggc
    15961 aaagaaatca ccatttcgat tcctttgttt ttcttttgcc aaaataaatt atttttgtta
    16021 tttatgtact tctgttgata cttttcaatt tcctcttcgg tcagttgact caacgatcca
    16081 ttgattactc cccatttacc agccaaactg actaaatcat aattattatc atccataaca
    16141 atcgtttcta taaaatttag ttctttcc
//
LOCUS       DADXTG010000057        15950 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.058, whole genome shotgun sequence.
ACCESSION   DADXTG010000057 DADXTG010000000
VERSION     DADXTG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.058"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            77..241
                     /locus_tag="IWI57_001551"
                     /pseudo
     CDS             77..241
                     /locus_tag="IWI57_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            489..731
                     /locus_tag="IWI57_001552"
     CDS             489..731
                     /locus_tag="IWI57_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449699.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKDLLIKVK"
     gene            complement(836..2044)
                     /locus_tag="IWI57_001553"
     CDS             complement(836..2044)
                     /locus_tag="IWI57_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HBC2449700.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2334..3299
                     /locus_tag="IWI57_001554"
     CDS             2334..3299
                     /locus_tag="IWI57_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449701.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3321..3533
                     /locus_tag="IWI57_001555"
     CDS             3321..3533
                     /locus_tag="IWI57_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBC2449702.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3608..4084
                     /locus_tag="IWI57_001556"
     CDS             3608..4084
                     /locus_tag="IWI57_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBC2449703.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4081..4581
                     /locus_tag="IWI57_001557"
     CDS             4081..4581
                     /locus_tag="IWI57_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HBC2449704.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            5141..6958
                     /locus_tag="IWI57_001558"
     CDS             5141..6958
                     /locus_tag="IWI57_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBC2449705.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7064..8392
                     /locus_tag="IWI57_001559"
                     /pseudo
     CDS             7064..8392
                     /locus_tag="IWI57_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            8469..8630
                     /locus_tag="IWI57_001560"
     CDS             8469..8630
                     /locus_tag="IWI57_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HBC2449706.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8671..9051
                     /locus_tag="IWI57_001561"
     CDS             8671..9051
                     /locus_tag="IWI57_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HBC2449707.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9066..9347
                     /locus_tag="IWI57_001562"
     CDS             9066..9347
                     /locus_tag="IWI57_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449708.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9606..10388
                     /locus_tag="IWI57_001563"
     CDS             9606..10388
                     /locus_tag="IWI57_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBC2449709.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10561..12279
                     /locus_tag="IWI57_001564"
     CDS             10561..12279
                     /locus_tag="IWI57_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBC2449710.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12281..13135
                     /locus_tag="IWI57_001565"
     CDS             12281..13135
                     /locus_tag="IWI57_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBC2449711.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13811..14131
                     /locus_tag="IWI57_001566"
     CDS             13811..14131
                     /locus_tag="IWI57_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449712.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14155..14565
                     /locus_tag="IWI57_001567"
     CDS             14155..14565
                     /locus_tag="IWI57_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBC2449713.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14712..14918
                     /locus_tag="IWI57_001568"
     CDS             14712..14918
                     /locus_tag="IWI57_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2449714.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15265..15786
                     /locus_tag="IWI57_001569"
     CDS             15265..15786
                     /locus_tag="IWI57_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449715.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
BASE COUNT         5025 a         3157 c         3937 g         3831 t
ORIGIN      
        1 tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat
       61 tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc
      121 gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa
      181 aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttcctcaaa
      241 attttaggtt atcgctgaaa gccccggaaa tacgggcatt tcgcaggata tgagaactca
      301 atttactacc aatttactac tttcggattg agccgcatat atgctgaacc atactgctaa
      361 tacccgccac cccaaatacg gcacatcata aattgaatac gacaccgtaa acgcggcgtt
      421 cctctttccc gtcatgggag aacaggacgc cgctttttta tgcccaaata gagaaaggag
      481 gcgacgccat gctatttact tccggccctg accgggcgtt tgaacggctg atgcaggaaa
      541 agcccggttt cgatcactac gacggcggcg gggcggacgc gccggaggat tgcggcacct
      601 gccgatttta ccgcccggat tggaaatatc aattctgcca gtttgcagag tgtccctatc
      661 agccgggcaa gctgaccgac tatgcagcgg tcaaattcac agtgaaagac ctgctaataa
      721 aagtcaagta gaaatttcag atttttaggg aggcgaaaaa atgcaataca aaatcaagcc
      781 gctgagcatc tcaaaattaa aacggcggcc agccagatgg tatagagtgt aaaaatcagt
      841 cgtgctccag ggattccaag taggctttca cagaagcgta gtagatgcgc aggaacttgt
      901 tggcagaggc catcatgtag acacgatatg gcttgccttc agagcgtttc ttgtccatga
      961 actggtacac cggctcgtct attggagcac attgcaggat gacgctcatc accagaaaga
     1021 gcgtcctgcg cagtgaggca gatcctcgct tggaaatgct acggctacgg acatctattt
     1081 gaccggattg gtagggcggg gcgtcaatac ccgcaaaggc aaccaacgct tttttggaat
     1141 gaaaacggcg tacatcgcca atttcagcca ggagttgagg gccgagggtt gggccaacac
     1201 caaacatccc catcactaca ggatactccg gcagagaagc tgccagggat tgcatctcct
     1261 gtttgagagc agctaacgcg gaagaagttg tctggagttg agaaatggcc tgttctacca
     1321 aaagttttgc cgtatccgtt ttcggtatga caccgaagtg tctgcaagca gaggcataaa
     1381 tatccagcgc cttatcctcg ctgaaattgt agccgtgctt tctgcaccac ttctggtatt
     1441 gggtagtaaa ggccttctca gaccggccac aaacgcactc gcaatgccaa aaagtagcga
     1501 caaagtccac ccacttctcg ctgccatcgg cgcggggcgg actggtaaac aagcgatttg
     1561 cgtctgggaa ggtggtgtcc agcagggaga tcaggttatt cttcagcatg gtctgaactt
     1621 tggaatactg ttggtactgc cggtagcaga ttttcagcat gagtcgggta tcctcctccg
     1681 ggatatatct cggcaatgtg agccagtggt caagaccgta gttggccagc tttacggcat
     1741 cctttttgtc ggttttggcc cgtcttaaac tgttgtttcc gtagtcatgc accagcattg
     1801 cattgactac cgagacataa aggcccgcgt cgtggagcag ccaggccacc ggagcatggt
     1861 aattacccgt ggattccatc accacacggg tctcgccgtc caggcttttg agcagtcctg
     1921 ccagctcgct cagttcattg gcggtgtgac gtacttcaaa gggtgaaacc actacctctc
     1981 cgaagggccg catgactgca atcatgctct tacccttgga aacatcgatg ccaacgcagt
     2041 tcattcacat tcctccaata caaagaatct gcaaccggaa tccatctttt ctcgctgccg
     2101 attcaatcta ttgtgtgaca cgaactctcc gacatccggt ggttcaacct gcttaaatcg
     2161 aacgctgcaa cgagaggatg gctaacagtc tttcacacgg acatacaagc ccaaggagtg
     2221 attggtcagc cagttgctcc tctcattgta gcttaggcac gagatggagg gaaagccgga
     2281 ctggctgtcc ggctttcctg tccaaattta ttgtaatagg agcgaacaac gctatggcag
     2341 ttttccgtgt ggaaaagaac aaaggctata cggtgatgtc aaatcaccac ctgcggaata
     2401 agaacctgac cttaaaggcc aagggcctgc tgtcgcagat gttgtccttg ccggaggatt
     2461 gggactatac cctaaaagga ctgtcctata tcaaccggga aagcatcgac gcgatcagga
     2521 cagcggtatg ggagcttgaa aaagctggat atatccggcg tgagcagggc cgtgacgcaa
     2581 aaggcaaaat ggccgatatg gtctatacca tctacgaaca gccggtattg gaaaacccgg
     2641 tattggaaaa tccaacatcg gataacccgg tgttggaaaa tccgacatcg gataatccgg
     2701 cgtcggaaaa tccaacgcaa ttaaataaag aagtacaaag aactgactta tcaaaaaaag
     2761 aaaaaataat tacagatgga caaagtaccc attccattcc tatcccatcc ccgacccctg
     2821 cccctatgga gggcgaggca gcggctacgc cgccggaacg gaaaaggaca ggaaaggatg
     2881 aggctgtacg gatttatcgg gaaatcattt tggagaacat cgagtatgcc tatctcattc
     2941 aggacaggag cattgaccgt gaacagcttg acgaaatcgt agacctgatg ctggaaaccg
     3001 tctgcaccgc ccgaaagaca atccgtgtcg ccggggacga ctaccctgcc gagctggtga
     3061 aatccaagta catgaagctg accggcgaac atatccgctt tgtccttgac tgtctgcggg
     3121 agaacacaac gaaagtccgc aatatcaaac aatacctgcg ggctatgctg ttcaacgccc
     3181 ccagcaccat ttccaactac tacacggccc ttgtcgcgca cgacatggca cagcccgatt
     3241 ggggaaagcc taaatccggc ctgccggact attcctgcgg cgagggcgaa agcctgtaac
     3301 gagaaaagga ggacgaccct atgaagcagg gcgcattgat ttttgacgag cgaacagacc
     3361 gttacgacat ccgctttgat ctggccgact attacggcgg cctgcattgc ggggagtgtt
     3421 tcgacgtgat gatcggcggc aaatggaggc ccacccgcat tgaaatggcc gaaaactggt
     3481 atcttgtcgg catccgggcc gacgaccttt ccggcctgcg ggtgcggatt tgacctatat
     3541 ctacctgcgg cgtctggtgg ttccgcctta gacgccgctt ttgactttct gacaaggagg
     3601 gatgcgattg caggaagaag ttactcaaaa gaccattgcc ctgtccatga aaacgggcaa
     3661 gctcacggct caagtgctgc aagcggcgct gaaaaagtac ctgcaacacc gggccaaggg
     3721 caaaaacacg ctgcaccacg gccagcagag cctaaagcag cttaaaaagc atggggctga
     3781 tctctccaac attgagatca ccgaggccaa cattggagcg ttcaagccat gcgccaagaa
     3841 atacggcgtg gattttacct tacgaaaaga taagaccacc cagccgcccc actacgttgt
     3901 gattttcaag gccaaggatg cagataacct tgatctggcg tttcgggagt ttaccgccaa
     3961 gacgctttcc aaggagcagc ggccctctat ccgcaaggtt ttgtccgctg tgaagcaaaa
     4021 ggcggcggcc cagaccaagc agcgggccaa ggagaaaatc aaggaaaggg ggctggaact
     4081 gtgaagcctg acgtaaagaa attgctgatc ctgaacctcc cgtatctgat ttttgtgtac
     4141 ctgtttgcga aatgcgggga ggcgtaccgg ctggccgctg gtgctgacct gtcggggaag
     4201 ctcctgcatt ttgcggatgg ttttgcggcg gcttttgcca acccgctgcc aagcctgcac
     4261 ctgtttgacc tgtgtatcgg tatcgccggt gccgtgatcg tgcggctggt ggtgtactgc
     4321 aagagcaaga acgtgaagaa ataccgcaaa ggcatggagt atggcagcgc ccgctggggg
     4381 acggccaaag acatagcgcc ttacatcgac cccaagtttg aaaataatat cctgctcacc
     4441 caaacggaac ggcttaccat gaccggcagg cccaaagacc ccaagacagc gcggaataaa
     4501 aacgtgctgg tgatcggtgg cagcggttca gggaaaacga gatttttcgt gaaacccagt
     4561 gcgcccgtaa gggcggtata aatcctacct tgagcaagta gtaggtaaaa gtatcaagcg
     4621 gcattgtgcc gcaacctatc attccccgtc ttaccccaaa agggaaaccg gagggcgacc
     4681 atagcatgac ggaggacacg gggcgctgaa gcctcagaag cgccggcgtg acggaatgaa
     4741 aatccacgta tgaggatgga agctagactg cttgaatgac agcctgaaat cgggaccaag
     4801 agcgtacccc tgacgtagag aggttgtact cgtcagtgtt cctgacagtc gcattgtttg
     4861 gctatgcggc tgctgatact cggagcaggt tcagccacgg gaaagtggaa aaaggcgaac
     4921 gtcacatacc gacaacgtgg aacgcttgtt tataccgtac taaacgggga ttgcctaaag
     4981 tgaaatgccg aaaggctatg aaaacgctga aatgcggggt tctgaatatt caccatggca
     5041 acagagttcc catagtagtc tgcggacggg aaagccgtct acatggcaaa ggggaacagt
     5101 gaatcatttt caaaacagaa aggatggtgt gtgagacact atgagaaatc cgattcatgt
     5161 cttgaaaagc cttgaagaaa aggcaagtgt aagtaactac aaatatgaaa gattatatcg
     5221 caacctatac aatccagagt tctatctcct tgcatatgcg aacattgcga aatcgcaggg
     5281 gagcatgacg cagggggtag acgggcagac gctggacaac atgagcctgc cgagaattaa
     5341 ccggattatt gaatccatac gcaacaggac atatcagcca aagcctgcaa aaagaaagta
     5401 cattcctaaa aagaacggga aacttcgccc gttgggaatc acttctactg atgataagtt
     5461 agtgcaggaa gtggtcagaa tgattcttga agcaatctat gagccgacat tcagcaacaa
     5521 ttcacacggt ttcagaccaa aaagaagctg tcacacggca cttacgcaag tgaagaaaaa
     5581 cttcacaggt gttacatgga ttgtcgaggg agacattaag gcgtgctttg ataacttcga
     5641 ccatcatgtg ttggttgaat tactgcggaa aaggatttca gacgaggctt ttatcggtct
     5701 aatctggaag ttcctgaaag ccggatatat ggaacaatgg cagtacaact gcacctactc
     5761 cggtgttccg cagggcagcg gtatcagtcc gatatgtgca aacatctacc tcagcgaact
     5821 ggacaactat atgcaggaat acaaagaaaa gtatgattgc gagccagaac gcagaaggac
     5881 gaccagagaa tacgagcggg cgtcccggag atacagaaag gcacgtaaag cgttaatggg
     5941 cgcagaaaaa tcaactcctg aactggtaaa agaatttaag gattccagaa ggaagaagat
     6001 gaatcagcac tattacaatc cgtttgagga aggcttcaag aaaatccagt acaaccgtta
     6061 cgccgatgat tttgtaatcg gcgttatcgg ctccaaaaag gacgcagaaa aaatcaagga
     6121 agatgtaaaa atctttctcc aagaaaaact gcatttggaa atgtccgagg aaaagacgaa
     6181 agtcacccat tccagtaagc cggtacgcta tctggggtac gatttcaaag taatccattc
     6241 caagaacatg aagcgttgta aaaacggcga catgaaacgg gtgtggtatg gaaaagtatt
     6301 cctgtatatg ccgaaagaaa aatggattaa aaaagcgatg gaacggggag cgatacaggt
     6361 gaaacgcaac aatgacacag gcaaagaaat gtggcggcct atgccaagga aagacctgat
     6421 gaaccgcagc gacgcagaga ttgtgtctac tttcaattcc gaaattcgag ggttatataa
     6481 cttctatcgg atagcagaaa acgtaggcgc attgcacaag tattactaca tggtgcggta
     6541 cagcatgtta aaaactctgg caggaaagca cagaacgaat gtcagtgtta ttaagaagcg
     6601 gcacatggta aacggagtac tgagaatccc atacgataca actaagggac gtaaatactg
     6661 cgaattttac catgatggat tcagaaagca cagcgacggc tatgacaatg tggcagacgt
     6721 tatgccgagt tataggaaat atgacagcag gcacacgata gtaaaccgca taaaagccgg
     6781 agtatgcgag atttgcgggg aacatgcaga ctatttatgt atgcaccacg taagaacgct
     6841 gaaatcgctg aaaggcaggg atatatttga gcagaaaatg ctgaaaatga gaagaaaatc
     6901 tctggctctc tgccctgact gctttgaact cttacacgaa accaaagaat caaggtgatt
     6961 catggaaagc cggatacgct gagaggtgta cgtccggttt gggaggcggc tccctgaaac
     7021 ctactgatga aagtcagcaa ggcgcagggt gcctacctca tacctgatgc aatgctttcc
     7081 gaccacggac tatccgactt cgtttgtggt gacagatccg aaaggcactt tggttctgga
     7141 aaccggcagg atgttccagc aggcgggcta ccgcattaaa atcctgaata ccattaactt
     7201 ttccaagtcc atgaagtaca acccctttgt ctacatccat tcggaaaagg acgtgctaaa
     7261 gctggtaaat accctgattt ccaacaccaa gggcgagggg gaaaagtcag cggaggattt
     7321 ttgggtgaag tcggaacggt tgttttattc ggccctgatc ggctatatct ggtacgaggc
     7381 cccagcggag gaaatgaact tctcaacgct gctggaaatg ataaacgcca gtgaagcccg
     7441 cgaggacgac ccggagtttc aaagccccgt cgatctcatg tttgagcgtt tggagcagaa
     7501 agaccccgac cattttgcgg tgcgccagta caagaaattc ctgctgtccg caggcaagac
     7561 ccgttcttcc atcctgattt catgcggtgc cagacttgcg ccctttgaca tccgggaggt
     7621 acgcgagctg atggaggacg acgaaatgga gctggacacc atcggggacg aaaagacggt
     7681 gctgtatctc attatgagcg atacggacac gacctttaat ttcattctgg ctatgctcca
     7741 aagccagcta atcaacctgc tttgtgaccg ggctgatgat gtgtacggcg ggcggctgcc
     7801 tgtccatgta cgcctgattt tggatgaatt tgccaacatt ggccaaatac ccaatttcga
     7861 taaactcatt gccaccattc gaagccgtga aatatcggcc tctattattt tgcagagcca
     7921 gtcgcagcta aaagcgattt acaaggacgc cgccgagatc atttcggata actgcgatag
     7981 tgtgcttttc cttagtgggc gcggcaaaaa tgccaaggag atttccgatg cgctggggaa
     8041 agaaacgatt gacagtttca acaccagcga gaaccgggga agccaaacgt cccacggatt
     8101 gaactatcaa aaattaggaa aggagttgat gtcccaagac gaaattgccg tgatggacgg
     8161 cggcaagtgt atcttgcagc tacggggtgt gcgcccgttt ttatcggaga aattcgatat
     8221 taccaagcat ccccggtaca aataccttgc cgacgccgac aagaagaata cctttgacgt
     8281 ggacagattt ttgactatca cgcgccggaa acggcagcaa gtggtaacgc aggatgaagt
     8341 attcgacctg tatgaaatcg acctgtcgga cgaggacgcc gccgctgaat aaacagcggc
     8401 ttttttcatt ccaacaacaa aatcaaaaaa tcattatgag ccggaaaccg gcagaaagag
     8461 gtaaatttat ggcatttttt aattccgcag ttactacttt gcagactatc gtgatcggac
     8521 tgggcggcgc tctgtgcgta tggggcggca tcaatctgtt ggagggatac gggcaggaca
     8581 atccgggggc aaaatcgcag ggtgtcaagc acttaatgtc ttctatgtga aaagatttta
     8641 aagagaaaat gcacatccta cgcagataac atgacaattt catgcaaagc gaaaggagac
     8701 accatgaaaa tcgaatacac aaaagtggga gactattatt taccgaatct ttactacccg
     8761 gaggaaacca gaccgattgg cttatgggga atgcttcgga aggaatatct gaaagaacat
     8821 aaatctggaa cttatacata tcttcttctg accgccaggc tggacagtta tttggcagat
     8881 ctgaatgaac aggcacagga acgctttgaa ttgatcgaaa ctcagatgcg aagtgcggag
     8941 ggagtttccg aggaactgaa gagacagaat cctatggaat ggctatgcca ctgtaacaat
     9001 atccgcaacc gggctgctga gatcatcaag caggaactga tttatgtata agggggattc
     9061 tagatatgag aagagaactg attgaaagtt attatgaaga ataccagcgg gagctggaaa
     9121 gatttcttga atctgacccg gagatgttca ctctggaagc agaaattcat cattacatag
     9181 aaatactgga accggtcctc agagatggcg gacaggagat gtggcagaca ctggacagga
     9241 ttatttcagc gaaaaatgca atggagggaa ttgtggcaaa agaaatgtat atccggggat
     9301 ttctggatta tgaaagactg gtgtgtgaaa aagacagtaa gatctgaata atcagaacat
     9361 agactatata taacgaggct tttattgtat agacagtaag agcctcattt aatatagttt
     9421 tcataataaa aaacaggtgg tagtacgtat ttgctttgtg ttatataaga attggtctgg
     9481 ctgaacaatg gtgaaaattt actatgattt tacttaaatc tgattgaagg atgaaaaagt
     9541 gatgatatca tatagtgggt tgatagggtt acagaaaatg tattcacaga ggcagagaag
     9601 aaataatgaa agtaaaaaat gcagatgaat tttataaaat attagaaaga aaggctatat
     9661 atccggtatt tcagccgatt gtaaatttac agactggaga tgtggcagga tatgaagctc
     9721 ttagtcgtat cgacaggcat gatacgactc ttatgatttc agatttgttt gtgattgctg
     9781 aacaggtagg ctgtgtttgg aaattggaaa aattgtgcag aaataaagca ttgaaggcag
     9841 cggcgaataa accagaacat gcaaaattat ttcttaatgt agatggtaat atcattcagg
     9901 ataaatcgtt catacaggga tttacaaacc gtaaggctgc gaaagccgga gtaccgtcct
     9961 gtgacattgt atttgaaata acggaacggt cggatataga aaactatcag attctgcaac
    10021 agattatgaa gcattatgca gatcagggat atgaaatagc tttggatgat gttggagctg
    10081 gatattctgg tctgaaccgt gtagtaaata catcgccaaa ctacttaaaa gttgatatag
    10141 aactggtcag agatattcaa aaacataaga aaaaggaaat tatgatggaa tttctgttgc
    10201 attactgtaa tgagacagga gcaattctga ttgcagaagg gatagaaaca gaagcagagt
    10261 tggagtgtct gtgtagatta ggtgttcatt acggacaagg atattttctt ggaagaccgg
    10321 acagaacgtt caaaaatgta aggaatgagg cgatgttggt attacgtaga ctgaataata
    10381 aatcgtaatg aaaactatat gtcagcaggt gaaaaatgaa taagtcaatt ataacattat
    10441 aaagatttcc attgattttt acacctattt tacacaagca ctcacataga tttgatttct
    10501 gtcttctata attgtatagg aaaatgtaga tcttatataa taacaggaga ggaaagagaa
    10561 atgaacgaga atgtgaaagt ggtttttggt ctgatcggtg gccttgcctt gttcctttat
    10621 ggaatgaaca gcatgagtga tgcacttcag aaagccgcag gagaacgaat gaaaaaaatc
    10681 cttggatttc ttacaagaaa tccaattatg ggagcacttg ccggagcact tgtaacagct
    10741 gtattgcaga gcagttcagc gacgacagtt atggttatcg gttttgtcag tgcgggactt
    10801 atgagtcttc cgcaggcaat ttctgttatc tttggtgcca acataggaac cactatgacg
    10861 gcacagctga tggcatttaa aatcagcaat tatatttatc caattatctt tgttggattt
    10921 attctgaact ttgttagcaa aaaagaaaag gttaaaaata tcggtatggt aattttttca
    10981 ttcggacttt tatttgaagg aattgaaatc atgggtgaag tcatgaaacc tttggcggga
    11041 agtccggtct ttgttgatct gatgggaaaa gtttcatcca ttccggttct tggtgttgta
    11101 ctgggagcgg tcatgacatt ggttgtgcag agtagttcag ctacgattgc agttctgcag
    11161 aattttgcat cacaggcagg accggacgga gtaagcagtg taatagggct taccggtgcg
    11221 attccgattc ttcttggaga taatataggt acaacgatca cagcacttct tgcatcaatt
    11281 ggacaatcta aaaatgcaaa acgtacagca attgcgcata gtatttttaa tatcagtgga
    11341 agctgcgttt ttattttcct ggttccgtgg tttgcaaaat ttgtccagtt tatttcacca
    11401 aagggaaatg agattgatgt gatttcaaga cagattgcaa atgcacatac aacgtttaat
    11461 atcgtctgta cactggtctg gctgccactg attccgctga tggtaaaaat tgttacaaca
    11521 atcatccgtg gaaatgacaa aacagagaaa gcagcttttg aacccaaata tctggatatg
    11581 aaagtgattg aacagccggc ggctgctatg gttctggtat ctaaggaact gaaccgactt
    11641 ggagaactgg cagaatctct gttaagtgat ttaaaaacgg cgattgtagc agatggagac
    11701 tcaaaaactc atggttcgtt tatagaaaat ctggagattg tacatcagtt acaggacagt
    11761 gtgacagaat atattaccag attattcgca agcggaaacc ttacagaaca acagtctgag
    11821 cagacggcag ggttactgta tgtaaacaat agtattcaga ggattgcaga cagatgtgaa
    11881 gatattgacc agatatgtga aaaagtaaat aacaatggaa aaagactgac cagtgaagcc
    11941 tcctcagaaa tggaagactg cattgatatt atccagcagc ttttaaagaa agcaatggag
    12001 gcaatccgta agggtgatgc tgtagtggct gaaacagttt ttaaaaataa gaaaaaaatg
    12061 cataaagcag agaaaaaata cagtaaggca catttaaacc gtgtaaaaaa tgaggtttgt
    12121 gatgcgtcta tgacaagata tttttctggg atcatgtata attttgaccg tatggcagat
    12181 aattgtgtca gcattgcaga agaagcctgt gataatgtgg cttttataaa ccttgatgaa
    12241 gagacgggaa aatcaatgat ggaaagaggt gcagtataaa atggtaaaga aaaaaaacat
    12301 ccgaaaatct gcacttctgc tgataatgat aatgctggca acgggaataa cagcatgtgg
    12361 aaccgaggaa gagccattgc cggatctgtc tgctatggga gctgtcacag ctgtatcacg
    12421 agaagaaggg tcaggaacca gagcagaatt tgagaatctt ttgaaactgc ctgagagtga
    12481 tacaggaata gttgttgatt ctacagagaa agttctgaaa aaggtggaag aagataaaaa
    12541 cgcagttggt tatgtcgcat atagttccgc aacagatacg aatggaaaaa tacttcaaat
    12601 caacggagtt cttccttctg agaaaacaat agacaataac agttatcctc tttgcaggga
    12661 ttattatctg gcttataatg gagaactgac agatgtggaa caggatttcc tgacttatgt
    12721 aaagagtaaa ggacaggata ttgtgaaaca atattgtatt caggctgaca gtacaacaac
    12781 attcctttct gacaagtcag aaggaaagat tctgattgaa ggatccactt caatggaacc
    12841 aatggtaaaa gcactggcag atgactatca gaaacaaaat ccaaatgctg aaatagaagt
    12901 aaaagcaaca gattcttcaa gagggattac ggcggtgata agtggagagt gtgatttcgc
    12961 aatgtcatcc agagaattaa aggattatga agcagagctt ctggaaacaa aagtcattgg
    13021 aaaagatgct atagctattg tgataaatga agaaaatcca ctggaaaatc ttacaataaa
    13081 acaacttaca ggtctgtaca atggaacata taaaaactgg gatgatcttt cctgagaaaa
    13141 acgaatataa tatcttacaa gcactgacta taacggtcgg tgcttctttt ttggattttt
    13201 tcaaaattat ctgacattct tcaaattccc acgtgtgatt agtggaaaaa taattttttc
    13261 tgcaaaagta ccgtccaaat gccctctgaa acttgctatt agtgagggaa tatttcattt
    13321 atgcgaaaaa aacttgcaaa agggggttgg taaataccgc ttcccaggct ttataggtag
    13381 aggaacaatt attttaaaaa ttttacccca aagatgtccg cttttgcctg tctcagtaca
    13441 gagtatgtga agggactaac aaaagtaaaa gaaaaaatga taaaaatgca aaaaaatatc
    13501 acgaatcttt tgcatcttgc gaagtcattt gactctagta atcagaggac gttttggaat
    13561 gttccttcga tgaaccttga caaccgataa gtgcacccta gaagcctaac aggagttatg
    13621 ccatgacctt tctttttctg agagcgagcg aaattataat gacactcccg atgatgggcg
    13681 gctgaccgaa gccttatggt cagtgacctg ctgatgcagg cagaggcagt ctgagacggc
    13741 tgcaggtgac agggaaacgc acgccagacc gccggacgaa tgaaactgat gacaatgttt
    13801 acagggcaaa atgcccggaa aggagcagtt tatgacagaa ctgaaaatga aagaaatgca
    13861 gttgattaac tggtttggat gcccgaatct gatttataca agggagagaa tccatctggt
    13921 tgccatgctt acaacagatg aagaaatgaa aaagaatctt tacagggtat gcagattcct
    13981 tgcaagagaa gaaatcgcat atcggtatcc gatcatgtat gaaatgatcc ggaaaatatt
    14041 ttcaccggat gataccaatc caccggaagc atgtgcaatg tttatgccgg acgatgaaac
    14101 agatgatgaa gaatatgcaa tggcagcgta gctgcggaaa ggacagtaac gattatgaat
    14161 aaagtggttt taatgggaag actgacaaag aatccggaga tcaaatatgc cggaaaagat
    14221 aatgatatgg cagtagcaag atatacactg gcggtaaaca gaagatataa acgtgacggt
    14281 gaacaggaag cggattttat ttcctgtgtc acattcggaa agagtgcaga gtttgcacag
    14341 aaatatcttc acaagggaat gaggattgtg attggcggtc gtatcagcac tggcaattat
    14401 aaggacaagg acgggaaaac aatttatact acagatgtga tcgtggaaga acatgaattt
    14461 gcccagaaca aggataatgg tgttggagct gattcatcgg aaacaccgaa aacagataag
    14521 gacggattta tggaagcacc ggaaggtgag gttccattcg actaaaccga agtaaatggt
    14581 tggagaggtt gccctttgtc ggggcatcct cttttccatt ttacagaaga catcaaaaat
    14641 tacaggtatt caacctaacg aaatcgtgat ataatcaaaa tatgttcaga tgaaaaatat
    14701 ggaggttgct tatgggcgta agttataaca gattatggaa aatgctcata gataaaaata
    14761 tgaagcggat agagatgcag tacctcaccg ggatcagtgg aaatattctt gccaggatgg
    14821 gaaaaaacca gtatgtatca atggagacga ttgagaagat ctgcaagaag ctggactgca
    14881 ccgttgatga gatgctggaa tttacagatg atgagtaaca agagcagaca tgtcaaatta
    14941 ttaaaactgg aagaatgctt cagaagtcga aaaaagaaat tgaaaaaaga taacacgccg
    15001 gatacggcat aaatctctta gcaacgctaa gagcgcactt ctatacaggc aattccatct
    15061 gcctgtatcg tgcgacctgc ggtgctgttc ccgatttcaa gaaatctcaa accgggaaat
    15121 acagcgaaat cagagatttc gtacaagggg aagatcccct tgcggcacag atgcctatcc
    15181 ctgataaagt ttgtgactga atatttcaga tatagacata taaggggaaa gaaagtgtga
    15241 aatcaaaatt tgtggagaga ttttatgatt agaaaagcaa tactaaaaga tgttaattcg
    15301 attgaagata cttacaatga gcatttccag tatgaactga atcatacagc ctttactgta
    15361 tttaagaaag gggtttatcc aacgaaagat gatgcagaac gggctattta tgctggtgct
    15421 ttgtttgtat atgaagaaaa cggaacaatt gttggaagta tcattattga taaagttcaa
    15481 ccaatagaat atgcaactat tccttggaaa gaaaaattat ctgaagatga ggtaatggta
    15541 attcaccttt taatggtacg tccgagtatg tctggtaaag gaattgcatc atcactaata
    15601 aaattcgcta cagaattagc acaaaaaaat agttgcaggg cactacgttt ggatacagga
    15661 agtcaaaata ttcctgcgct atctttgtat caaaaaaatg gatttgaaat aattgctagt
    15721 gcttctaaaa aagttggaga tgtaattgca cacaaaaacc atctttttct cgaaaagata
    15781 ttgtagttcg taaattccag tttgaaaaat tgagaaaatc ggagtttgag gagcatcacc
    15841 gttatgatta gagaattacg gaaagtggat ataaataaag ttgcagaaat atggttagat
    15901 acaaatataa agacacatta ttttatctct gctcaatatt ggaaaagtaa
//
LOCUS       DADXTG010000058        15077 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.059, whole genome shotgun sequence.
ACCESSION   DADXTG010000058 DADXTG010000000
VERSION     DADXTG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.059"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWI57_001570"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWI57_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBC2449716.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWI57_001571"
     CDS             complement(322..891)
                     /locus_tag="IWI57_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBC2449717.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWI57_001572"
     CDS             1077..1979
                     /locus_tag="IWI57_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2449718.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWI57_001573"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWI57_001573"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBC2449719.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWI57_001574"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWI57_001574"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBC2449720.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWI57_001575"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWI57_001575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBC2449721.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWI57_001576"
     CDS             8547..8732
                     /locus_tag="IWI57_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBC2449722.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWI57_001577"
     CDS             complement(8794..9990)
                     /locus_tag="IWI57_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBC2449723.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWI57_001578"
     CDS             complement(10160..11596)
                     /locus_tag="IWI57_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBC2449724.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTDILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWI57_001579"
     CDS             11889..12698
                     /locus_tag="IWI57_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2449725.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IWI57_001580"
     CDS             complement(12884..15037)
                     /locus_tag="IWI57_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBC2449726.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4399 a         3196 c         2665 g         4817 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatgtctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgta
//
LOCUS       DADXTG010000059        14606 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.060, whole genome shotgun sequence.
ACCESSION   DADXTG010000059 DADXTG010000000
VERSION     DADXTG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.060"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            48..860
                     /locus_tag="IWI57_001581"
     CDS             48..860
                     /locus_tag="IWI57_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBC2449727.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            857..1657
                     /locus_tag="IWI57_001582"
     CDS             857..1657
                     /locus_tag="IWI57_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBC2449728.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1818..3086
                     /gene="rseP"
                     /locus_tag="IWI57_001583"
     CDS             1818..3086
                     /gene="rseP"
                     /locus_tag="IWI57_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBC2449729.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3154..4863
                     /locus_tag="IWI57_001584"
     CDS             3154..4863
                     /locus_tag="IWI57_001584"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBC2449730.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5075..9427
                     /locus_tag="IWI57_001585"
     CDS             5075..9427
                     /locus_tag="IWI57_001585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBC2449731.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9571..10044
                     /gene="rimP"
                     /locus_tag="IWI57_001586"
     CDS             9571..10044
                     /gene="rimP"
                     /locus_tag="IWI57_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBC2449732.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10068..11243
                     /gene="nusA"
                     /locus_tag="IWI57_001587"
     CDS             10068..11243
                     /gene="nusA"
                     /locus_tag="IWI57_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBC2449733.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11265..11558
                     /locus_tag="IWI57_001588"
     CDS             11265..11558
                     /locus_tag="IWI57_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBC2449734.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11555..11866
                     /locus_tag="IWI57_001589"
     CDS             11555..11866
                     /locus_tag="IWI57_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBC2449735.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11879..14185
                     /gene="infB"
                     /locus_tag="IWI57_001590"
     CDS             11879..14185
                     /gene="infB"
                     /locus_tag="IWI57_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBC2449736.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14212..14559
                     /gene="rbfA"
                     /locus_tag="IWI57_001591"
     CDS             14212..14559
                     /gene="rbfA"
                     /locus_tag="IWI57_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBC2449737.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2578 c         3257 g         3789 t
ORIGIN      
        1 ataatactta agatagtggg aatcttttaa gtataggagg aaaaagaatg ttacgttttt
       61 ttccgcaaaa aaacaaatat attcaagaaa aaagcgaatt tcaatttaat accgatggac
      121 caattcctca gcacattgcc atcatcatgg acggcaatgg tcgttgggcg caaaaccgtc
      181 gtttgccacg agttgctggc cataaagaag gcatggaaac agtaaagaaa gtcacaaaaa
      241 aagcatcaag gcttggagtg aaagtgttga cgctttatgc cttttcgact gaaaactgga
      301 agcgaccaaa agatgaagta agttttctca tgcaactacc tgtcgacttc tttgatacgt
      361 tcgtaccaga attgattaaa gaaaatgtca aagttcatgt catgggttat gaaaacgtat
      421 tgccagaaca tacgcaagat gctgtaagac gtgcaattga gcaaacaaaa aacaatactg
      481 gaatggtatt gaattttgct ttgaattatg gaagtagagc agagatcgtg actgctgtcc
      541 aagaaattgc cgaagaagta gcaaaaggag aaatacacgc agaagaaatc gatgatgaat
      601 tgattgctaa gcatttaatg acaggtttct tgcctaaaga gttacaagat cctgagctga
      661 tgatccgtac aagtggagaa gagcgaatta gtaattttct gttatggcag atcgcatata
      721 gtgagctgta ttttacaaaa gcgctttggc ctgattttga tggtgcgcat cttgaagaag
      781 caatcgcctc ttatcaaaat cgagaccgtc gattcggcgg tgtaaaaaaa acagaagatg
      841 agaaagggga tcagtcatga aacaacgtgt gatcacagca gctgtggcgc tagtcttgtt
      901 tattccgatt atatggatcg gaggcatagc ggttgagctt acagcagcaa tcttagcggt
      961 agtaggagtt tacgagctgt tccggatgaa aggattgaca ctcttaagtt ttgaaggagt
     1021 tttatccgcc gttggtgcag tgttacttgt tttgccaaaa gaaagatggt ttttctttct
     1081 tccagagaat gtaagtacat ttatgttatt ttatcttata gttatgattc ttttaggaac
     1141 atcagttatt tctaaaaata cgtatacgat tgatgaagca ggttttcctg tgcttgtcag
     1201 cttgtatgtg ggaataggtt tccagaattt tgtcaatgca cgaacagaaa gcttggctgt
     1261 tttgatattt ggtctattta ttgtttgggc aactgatatc ggagcatata tggttggtcg
     1321 acgttatggt cagcataagt taagtcctga tatttcgccc aacaaaacga ttgaaggtgc
     1381 gctcggtggt atcggtagtg ctgttgtagt cgcatttctt tatttattgc tatacccagc
     1441 aaaagaacta ttcaatcatg gtatgtttgt catgcttctg atgacgatta ttttttcagc
     1501 agtcggacaa tttggtgatt tggtcgaatc tgcaatcaaa cgtcattatg atgtgaaaga
     1561 ctcgggaaat atcttaccag gccatggtgg aatcttagac cgtttcgaca gtttgctgtt
     1621 tgtatttcct atcatgcatt tatttggcat tatttagtaa gtatacctgc gtttctttgt
     1681 gaatttcata aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg
     1741 gtatactatg gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag
     1801 taaaggagaa aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag
     1861 tgattgttca tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgtccgcg
     1921 aatttgctat cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat
     1981 atacgcttcg cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg
     2041 aaacagaaat ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag
     2101 agaagataaa tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc
     2161 gttatgattt agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag
     2221 tagtaaccta ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca
     2281 gaattgcgcc aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca
     2341 actttgctgg acccatgaat aactttattt tggcaatcgt gttattcatc attctggcat
     2401 ttatgcaagg tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg
     2461 gagctgcggc agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag
     2521 aaatccattc atggaatgat ctgacaacag tcattacgaa aaatccaggt aaaacattag
     2581 actttaagat cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag
     2641 agtcaaatgg cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta
     2701 tggataagat cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag
     2761 ccttaggatc actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt
     2821 accaattgtc ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc
     2881 ttctttcaat gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga
     2941 aattagtctt gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag
     3001 gaatattgac attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga
     3061 acgatattca acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga
     3121 taactatttt gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca
     3181 acattacgcg aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct
     3241 ggatatatcc gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta
     3301 ttggaaaaac tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg
     3361 ctgatgcctg ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat
     3421 ggaccaaact tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact
     3481 cacgaagaaa cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct
     3541 ttaaatcttt atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg
     3601 cttcgcggaa gagagtttat catgaaagat gcatattctt tccatgcaac ggaagaaagt
     3661 ttggacgaga catataaaga ttacgaaaaa gcctataccg aagtattcaa gcgttgtggc
     3721 ttagaatttc gctcgatcat cggagacggc ggcgcaatgg gcgggaaaga ctcaaaggaa
     3781 ttcatggcga tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat
     3841 gctgcaaact tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag
     3901 cttgaattgg agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc
     3961 ttttctgttg aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct
     4021 gtccttgttt tggtacgtgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta
     4081 tctgctgact ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag
     4141 tttggttctg tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat
     4201 gtccaagatt tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat
     4261 gtgaatccaa aacgcgattt cactgtgctt tcttatgaag atcttcgttt tgtacaagaa
     4321 ggagatcctt caccagacaa caacggtgtt cttgccttta ctcgaggaat cgaaatcgga
     4381 catatattca aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa
     4441 aatggacgag aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta
     4501 tcagcaatcg tagaacaaca ttcagatgaa caaggaatca attggccaaa aggaattgcg
     4561 ccatttgatc tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aagcctgacg
     4621 aatgaactag aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat
     4681 gaacgcgcag gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact
     4741 gtcggtaaaa aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg
     4801 gttgaagtac gtaaagaaga attggcgaat actttaccaa tcctcttgaa ccaaggcgaa
     4861 taataagcgt taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga
     4921 tgattcgctt ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa
     4981 actcagaaaa aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa
     5041 ttgcattgtt tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga
     5101 aaaactgttg gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc
     5161 tggtgaaatg gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctaca
     5221 tttttccaaa attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc
     5281 ttttaaagat attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga
     5341 acagctcttg caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact
     5401 agcacagcaa gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc
     5461 aatcgacagt acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga
     5521 gttgttcgtc agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca
     5581 agcggaacgt gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat
     5641 gaagcaagca gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca
     5701 atcgccagct cttgaaggac ccatccagct aggacgaaat attccagccg atgaaccaac
     5761 gacaccaatg atcagtatcg ttgaagaaga acgaagagtc acacttgagg ggtacgtatt
     5821 cgacaaagaa gtcagagaac tacgttcaaa acgcaagatc ttgactttga aaatcacaga
     5881 ttatacttca tcatttatcg tcaaaaaatt ctcgaacggc gaaaaagatg aacaagtatt
     5941 tgatgcaatc agtgtcggta gctggttaaa ggtacgagga agtattcaag aagatacgtt
     6001 tgtccgtgat ttggtcatga atgcgcaaga tatcatagaa gtcaaacata cacctcgcaa
     6061 agattatgct cctgaagggg aaaaacgagt agagcttcat gttcatagca atatgagtac
     6121 gatggatgca acaaacagca tctctgatct tgtggcacaa gcaggaaaat ggggacaccg
     6181 agctatcgcc attacagacc atggtggtgc gcaagccttt ccggaagcac atagtgctgg
     6241 aaaaaaagca ggtgtgaaaa tcttatacgg cgtggaagcg aatgtcgtag atgatggtgt
     6301 cccaattgct tataatgatg cacatgaagc attgagtgaa gccacatacg tcgtatttga
     6361 cgtggaaacg acaggattgt ctgcagtata tgacacgatc atcgagctag ccgcagtcaa
     6421 aatgtataaa ggaaatgtga tcgaaagctt tgatgaattt atcgatccag gacatccatt
     6481 gtcacgaaca acggtagatc tgactggtat cacggatgaa atggttcgag ggtcaaagtc
     6541 ggaagaagaa gtattgcgga tgttccttga attttcaaaa gatacaattt tggtagccca
     6601 caacgcggct tttgatatgg ggttcttgaa tactagctac gcaaggtatg gtattcctga
     6661 agcggccaat ccagtcattg atacgttgga attagctaga tatctgtatc ctcaattcaa
     6721 acgtttcgga ttaggtgtgt tatctaaaaa gtttggtgtt agcttggaac agcatcaccg
     6781 agcaatctat gatgctgaag caactggtca cttagcatgg atctttgtga aagaggcaat
     6841 ggacaatcat aacatgcttt atcatgatca gctaaatgag catatcggag aaggagattc
     6901 ttacaaaagg gctcgacctt tccatgtaac gattttagca aaaaatcagg caggactgaa
     6961 agatttgttc aaactgattt caatgtcgaa tgtcgaatac ttcgaacgcg tcccacgaat
     7021 accacgctcc caattgaaaa agatgcgtga aaatctattg atcggctcag cttgtgataa
     7081 gggagaaata ttcgaagcca tgatgcaaaa aggggtagaa gaggcaagaa accgagcaaa
     7141 attctatgat tatattgaag ttatgccaaa agcggtctat gcgcctttga tcgagcaaga
     7201 attggtaaaa aatgaacatg atttggaaga aattatccaa aatttggtag aaatcggaaa
     7261 aagtttggat aaaatcgttg ttgcaacagg taatgtacat tatctgaacg aagaagatgc
     7321 catttaccga aaaattttga tcaattctat gggcggagcg aatccgttga atcgtcatag
     7381 tttgccggat gtccatttcc gaacaaccga tgagatgcta actgcatttc actttttagg
     7441 ggaagaaaca gcgaaagaga tcgtagtaga aaatacgaat aaaatcgcag atatctgcga
     7501 agaagtgatt ccagtcaaag atgaacttta tacaccgaag attccaggtt cagaagatga
     7561 aatatcagaa ttgagttaca caaaagcaaa acaaatgtac ggagatccgt tacctgagat
     7621 cattcaaaaa cgattagaaa aagagttgaa ttctattaat ggtaatggct tctcggttat
     7681 ttatctgatc gcccaaaaac tagtgcataa aagtaatgaa gatggctatc tagtaggttc
     7741 acgtggctct gttggctcta gctttgttgc gacgatgaca ggaatcactg aagttaatcc
     7801 attagcccct cattattatt gtccagagtg ccaatattcc gagttttttg aagatggtac
     7861 gtatggttct ggatttgaca tgccagaaaa acaatgtcca aaatgtggtg cacgcttaaa
     7921 caaagacggt catgatattc catttgagac atttttagga ttccatggag acaaagtgcc
     7981 cgatattgat ttgaacttct caggtgatta tcaagcagaa gcccataact atacgaaagt
     8041 attgtttggc gaagactatg tttaccgtgc cggaacgatt ggtacagtcg ccgacaaaac
     8101 agcatatggc tatgtaaaag gatacgaaag ggacaataat ctgcagtttc gaagtgcaga
     8161 agtcgacaga ttagcaaaag gcgctactgg agtcaaacgg acaaccggcc agcatccagg
     8221 ggggatcatc gttattcccg attatatgga tgtctatgat tttacgccga tccaatatcc
     8281 agcagacgat caaaattcag aatggaaaac gactcatttt gatttccact cgattcacga
     8341 taatgtattg aaattagata ttctagggca tgatgaccca actgttattc ggatgcttca
     8401 agatttatca gggatcgacc cacaaacgat ccctacggat gacccggaag tgatgcggat
     8461 atttgccggt cctgaagtac ttggagtaag tcaggaacaa atttattcca aaacagggac
     8521 attaggtatt ccagaattcg gcactcgttt tgttcgaggg atgttagaag aaacgcatcc
     8581 tacaactttt gcagaactac tgcaaatttc cggactatct catggtacag acgtatggtt
     8641 aggaaatgcg gaagaattga ttcgacgagg agatgcgaca ctggcggaag taatcggctg
     8701 tcgtgatgat attatggttt atttgatcca tgctggatta gatagcggga tggcatttaa
     8761 aatcatggaa actgttcgta aaggacaatg gaacaagatc cctgatgaat tgcgagaaac
     8821 ctatctatcg gcgatgaaag aaaataatgt gcccgactgg tatatcgatt cttgttcaaa
     8881 aatcaaatat atgtttccga aagcccatgc tgcagcttac gtattgatgg ctcttcgtgt
     8941 cgcttatttc aaggtttatt tccctatttt gtattactgt gcatactttt ctgtacgtgc
     9001 agacgacttt gatctagtct ccatgtgcaa aggaaaagat gctgtgaaac aagcaatgaa
     9061 agaaatcacg gacaagggat tagatgcttc cgtaaaagaa aagaatcaac tgacagtgct
     9121 ggagttagcc aatgaaatgc tggaacgagg attcaaattt ggaatgattg atttgtataa
     9181 atcggatgcc gttaactttg ttattgaagg tgatacgttg atcgcaccat ttagagctgt
     9241 cccaagtttg ggaaccaacg tggcgaaaca aatcgttgaa gcaagaaaag acggtccatt
     9301 tttatccaaa gaagatcttg ccacccgcgg taaagtttca aaaacattga tcgaatatat
     9361 gaacgacaat ggcgtattga aagatctacc agatgaaaat caattatcgt tattcgatat
     9421 gctataagca tctgttttcc tgtctgcttg taattttaag cagagtatgg tatagttctt
     9481 ataggtaatg atactaactg tgagtgagcg gatttttcgc tcactctttt taatggaatt
     9541 acgcacttaa tttttaagga ggcgaaactt ttgagtagcg tcgttgaaac agtcacagaa
     9601 atggtgacac cgattttaga tgaacaaaat tttgaacttg tcgaagtcga atttgtaaaa
     9661 gaaggaaaaa gctggttctt acgagtattt attgacaaag aaggcggtat cgatatcgaa
     9721 gaatgcgcct ttgtcagtga gaaactgagt gaaaaacttg atgcagcaga tccagatccg
     9781 attcctcaag cttatttcct ggaggtatcc tccccgggag cagagcggcc attgaaaaaa
     9841 gaaagcgact atgaaaaggc acttggtgat tatgtccatg tctccttata ccaaccagta
     9901 gatggcgaaa aacaatatga aggatttctt caatcatttg atgctgaaca gcttacattg
     9961 aaaatacgca taaaaacacg agaaaaagaa ttgacttttg accgaaaaaa tattgcaaaa
    10021 gctcgcttag ctatccaatt ttaatcacgg agaggaatca gaaaaaaatg agtaaagaaa
    10081 tgttaaatgc gttagacgct ttagaagctg aaaaagggat ctcaaaagaa atcgtgatcg
    10141 atgctttaga agcagcattg gtttctgcat acaaacgtca ctatggccaa gcacaaaacg
    10201 tcgaagtgga atttgatcag aaaaaaggaa agatccatgt ctatgcggta aaagaagtaa
    10261 cggaagaagt gatggattca cagttggaag tctcattgaa agatgcactt ttaatcaatc
    10321 cagcatatga gatcggtgat acgatccgtt ttgaagtaac accaaaagat tttggacgga
    10381 tcgctgcgca aacggctaaa caagttattt tacagcgtgt acgtgaagca gaaagaacga
    10441 ttatttataa cgaattcagt gcttatgaaa aagacatcat gcaaggaatc gtcgaacgcc
    10501 aggataaacg ttatatttat gtcaatcttg gtaaaattga ggcagttctt tctaaacaag
    10561 atcaaatgcc aaatgaattt tatcaaccac atgatcgaat caaagtttat gtttcccgag
    10621 tagaaaatac gtctaaaggt ccgcaagtat ttgttagccg cagccatcca gacctattgc
    10681 gtcgcttatt tgaacaagaa gtaccagaag tctatgatgg aatcgttgaa attgtcagca
    10741 tcgcccgtga agctggcgat cgttcaaaag tggcagttcg ctcaaatgat ccaaatatcg
    10801 atgcagtcgg tacatgtgtc gggccaaagg gacaacgagt tcaagcaatc gtcaatgaat
    10861 taaaaggcga aaacatggat atcgtcgaat ggaatgaaga tccagctgta ttcatcgcaa
    10921 atgcattgaa tcctgcacaa gtcatggatg tgatcttcga tgagacaaat ccaaaggcct
    10981 gcacagttgt cgttccagac taccaattat cgctagcaat cggtaaacgc ggacaaaatg
    11041 cacgtttagc tgctaaattg actaaccata aaatcgatat caaatcagaa tctgacatga
    11101 cggaatttta tgaaaatcaa gaacaacaaa aagaaacaga agaactgcat gatgaagcaa
    11161 tcgttcagtc agatttaaca gacgatgaat atgaaacaat tgcattcaac aatgaaacgg
    11221 tcgaagaaaa accagaagct taactttagt aaggaggcaa agagatgaaa caaaggaaaa
    11281 ttcctttacg caaatccgtg gtttcaggtg aaatgaaacc taaaaaagag atggtccgaa
    11341 tcacccgttc aaaagaaggg gaagtatcga tcgatccaac tggaaaaatg cctggacgag
    11401 gagcgtatgt ctcacttgag ccggaagaag tacaacaagc ttgggataaa aaaattctcg
    11461 atcgagtact cgaaacaaca ttaacagatg agttttatca agaacttctt gactatgtca
    11521 ctcaccaaaa agcacgtaaa gagttatttg gaaaatgaat acagttaatc ggcaaaaagc
    11581 catgaatctc atcggtctag cgatgcgcgc gggaaaacta atcacgggtg aagaactgac
    11641 cattgctgaa atccgcaaac aaaaagcaaa attggtgttt gtggcaaccg atgcaagtga
    11701 gaatacaaga aaaaagataa aagataagag ttcatattat gaagttcctt gctttgagct
    11761 attttctgaa gcagaaatca cgcagatgat cggaaaacct cgcaaggtat tcggaataat
    11821 ggatgccgga tttgcgaaga agaccaagga gctaattgaa ggttaggaag gtgattgcat
    11881 gggcaacaaa agaatctatg aattagcaaa agaactcaaa aaatctagta aagacgtcgt
    11941 ggaaaaagca caacaacttg gcatcgatgt gaaaaaccat atgggtgcga ttactagcag
    12001 tgatgagaag aaattgcaac aagcgttcaa aaacccaagc acgaaacaac aagctgcaca
    12061 acaagcaagc agaaaaccag ccaatcaaca gccgaacgat caacgtaaaa aagaaacaac
    12121 gaacaatcaa caaaaaaata aaaataaccg cagttatcaa gatcgcggtc aagggagcgg
    12181 tcaagtgaat caaggaaaaa aaccaacaac aaaccaaaat aatacgggaa ataccagcag
    12241 ccaaaatcgt caaggaacga gccaaggcgg caatcaaaac cgtactaaca ataacaacaa
    12301 accaaacaat aacaatcgcg gcaaatttaa taataataac cgtaatcgtt ataataaaaa
    12361 aggtaaaaaa ggcaaacagc aacaatcaac taaaccagct gtaccaccac gcaaattccg
    12421 tgaattgcca gatgtattag agtatactga aggtatgaac gtagcagata ttgcgaaaaa
    12481 aattcatcgt gagccagcag aaatcattaa aaaattattt atgcttggtg tcatggtcaa
    12541 tcaaaaccaa gcactggata aagatacgat tgaactgtta gcgacggact atggcatgga
    12601 accacaagaa aaagtccaag ttgacattgc tgatatcgat aaattcttcg aggcagaaga
    12661 agtcgatcca gataaattag tctcacgtcc gccagtagta acgattatgg gacacgtaga
    12721 ccacgggaaa acaaccttat tggatacatt gcgccattca cgtgtaacaa gcggcgaagc
    12781 cggcggaatc acacagcata tcggtgccta ccagatcgat attgatggta aaccaatcac
    12841 tttcttggat acaccaggac atgcggcctt tacaagtatg cgtgcacgtg gagcaagtat
    12901 cacagatatc acgatcttag ttgttgctgc agatgatggc gtcatgccac aaacagttga
    12961 agcaatcaac catgccaaag ctgcaggcgt tccaatcatc gtcgctgtaa ataaaattga
    13021 taaaccaggt gcaaatcctc agcatgttat gcaagaatta agtgagtatg aattaattcc
    13081 tgaagcatgg ggtggagaaa cgatttttgt tgagatctca gcgaaattcg gccaaaacat
    13141 cgaagaattg ctggaaatga tcctgttagt cgcagaagta gaagatttaa aagcagatcc
    13201 aacacaacgc gcaatcggaa cagtgatcga agcacgtctt gataaaggta aaggtcctgt
    13261 gtcaacctta cttgtccaag aaggaacact tcacgttggt gacccaatcg ttgtcggtaa
    13321 tacttacgga cgtgtccgtg taatgacaaa cgatcttggc cgtcgcgata aagaagcagg
    13381 acctgcaacg ccagtagaaa tcactggctt gaatgatgta cctcaagcag gtgaccgttt
    13441 cgtagtcttc gaagacgaaa agacagcacg tgcagcgggt gaagaacgcg caaaacgtgc
    13501 attattggaa aaccgtgctg ccacaagccg tgtgactttg gacaacttat ttgaaagcct
    13561 gaaagaaggc gaattgaaag aagttaacgt aatcatcaaa gcagatgtac aaggttcagc
    13621 agaagcactg gctgcatcat tgaagaagat tgatgtggaa ggtgtccgtg taaaaatcgt
    13681 ccattcagct gtcggagcaa tcaatgaaag tgatgtaacc ttagcagcag ccagcaacgc
    13741 tattatcatc ggtttcaacg ttcgtcctac accgcaggca aaagtgcaag ctgatactga
    13801 agaagtcgat attcgtcttc accgtatcat ctataaagcc atcgaagaaa tcgaaactgc
    13861 gatgaaaggg atgctagacc ctgaatttga agaaaaaatc accggtcaaa tgatcgttcg
    13921 tgaaaccttc aaagtttcta aagttggtac gattgcaggt gcctatgtaa cagaaggata
    13981 tattcgtaga gatagcggtg tccgtgtaat ccgtgacgga atcgtaatct atgaaggcca
    14041 acttgcaagt ctgaaacgat tcaaagatga cgtgaaagaa gtcaaaatgg gttatgaatg
    14101 tggtgcgatg attgaaaaat tcaatgacat caaagtagat gacgtaattg aaggatttat
    14161 catggaagaa gtcaaaaatg actaaaaact gataaaagga ggccaatcat tatggctaac
    14221 taccgtgatc gccgagtcgg tcaagaaatc ttaaaagaag tcaatgatat tttgcgcaaa
    14281 aaggtccgtg accctcgtgt ggagaacgta acgatcacag atgtgaaagt tacgggggac
    14341 ttacagcaag caacgatcta ttacagcatt ttatctgact tagcatcaga taagaaaaaa
    14401 gcacaagatg gattgaataa agcatcagga ttgattcgtc gagagttagg gcacaatcta
    14461 agtatttata agacacctga attgttcttt gaacttgatg aatcagttgt ctatgggaac
    14521 catatcgatg aattgcttcg cgacttgaac aaagactaat caaaaaatcc cgcaaatcct
    14581 gtgaaatcag gggttgcggg attttt
//
LOCUS       DADXTG010000060        14385 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.061, whole genome shotgun sequence.
ACCESSION   DADXTG010000060 DADXTG010000000
VERSION     DADXTG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.061"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..145
                     /locus_tag="IWI57_001592"
     CDS             14..145
                     /locus_tag="IWI57_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBC2449738.1"
                     /translation="MATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(207..2018)
                     /locus_tag="IWI57_001593"
     CDS             complement(207..2018)
                     /locus_tag="IWI57_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBC2449739.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2015..2380)
                     /locus_tag="IWI57_001594"
     CDS             complement(2015..2380)
                     /locus_tag="IWI57_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBC2449740.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2543..2938)
                     /locus_tag="IWI57_001595"
     CDS             complement(2543..2938)
                     /locus_tag="IWI57_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBC2449741.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2956..3918)
                     /locus_tag="IWI57_001596"
     CDS             complement(2956..3918)
                     /locus_tag="IWI57_001596"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBC2449742.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3918..4130)
                     /locus_tag="IWI57_001597"
     CDS             complement(3918..4130)
                     /locus_tag="IWI57_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBC2449743.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4151..4849)
                     /locus_tag="IWI57_001598"
     CDS             complement(4151..4849)
                     /locus_tag="IWI57_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBC2449744.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4869..5411)
                     /locus_tag="IWI57_001599"
     CDS             complement(4869..5411)
                     /locus_tag="IWI57_001599"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBC2449745.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5543..6547)
                     /locus_tag="IWI57_001600"
     CDS             complement(5543..6547)
                     /locus_tag="IWI57_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBC2449746.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6544..7533)
                     /locus_tag="IWI57_001601"
     CDS             complement(6544..7533)
                     /locus_tag="IWI57_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBC2449747.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7530..8336)
                     /locus_tag="IWI57_001602"
     CDS             complement(7530..8336)
                     /locus_tag="IWI57_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449748.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8502..9458
                     /locus_tag="IWI57_001603"
     CDS             8502..9458
                     /locus_tag="IWI57_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2449749.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9535..10053)
                     /locus_tag="IWI57_001604"
     CDS             complement(9535..10053)
                     /locus_tag="IWI57_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2449750.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10141..10290)
                     /gene="rpmG"
                     /locus_tag="IWI57_001605"
     CDS             complement(10141..10290)
                     /gene="rpmG"
                     /locus_tag="IWI57_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBC2449751.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10518..10964
                     /locus_tag="IWI57_001606"
     CDS             10518..10964
                     /locus_tag="IWI57_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBC2449752.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11159..13054)
                     /gene="asnB"
                     /locus_tag="IWI57_001607"
     CDS             complement(11159..13054)
                     /gene="asnB"
                     /locus_tag="IWI57_001607"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBC2449753.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13379..14353)
                     /gene="bsh"
                     /locus_tag="IWI57_001608"
     CDS             complement(13379..14353)
                     /gene="bsh"
                     /locus_tag="IWI57_001608"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBC2449754.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         4477 a         3033 c         2429 g         4446 t
ORIGIN      
        1 attagtagga agtctggcaa cagccgctgc cgttgctggt ttggttgctt ctgtgaaaaa
       61 aacagtgatc gatccaattg acgaaaaaga agcaatgatc gaagaaaacc gtaaaaaagc
      121 aatgcgtaaa cgcgtttctc gttaataatt atttaaaaat acgcatgaag actttctaaa
      181 aaacagataa gtcatcatgc gtatttttat ttgtcagtca atataagttg tgctatttta
      241 tctgcctgtc tcagattcca tggatctgta taaggtctag ctactgctgc agttagccaa
      301 tccacacctt caacaggtat tccatagcaa taaaagtcag actggatttc ccctgaagca
      361 tcaatcacgc gattcgtacc tcgctcaaca tctatagctt ctgaatgata acttcctgta
      421 ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct tcaaattttt tatgacagga
      481 ttacttgaac gagaaaagct tgtttgtggc aatcgagctt caatcaaata gcgactgctg
      541 atttctttat cttgtagaga aataacatag cggttgtgct tttgctgaat atcaaatggc
      601 ggttcgatca aatgaaaaat acccgcatct atcagtgctg ctaattcttt cgtccgaatg
      661 attggcggac cgatcgttaa aaagctattt agatgagtaa accatcccca tagtatttct
      721 ttgtattcct tagcagaaaa aacgtgccat tcaatagcct gccggattgg atcacgccag
      781 tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc aattgccttt ttccgcttct
      841 tttatctgcc cttcaataaa tattcggcta gcttctgaaa agggctcatt ctgataggca
      901 agaggagttt ctaaatacga ccaatcccat ctataaggga ttagtgctgg atgctttccg
      961 agacactcct ctgaagagag ggagagaaaa tcatgctcaa aatttttctt tggaatcggc
     1021 aagtgatatt cttcgatgat tttcttgtaa taaaacaact caacgtcttt tttcagatag
     1081 tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc attgctccag gttttctttt
     1141 gttagtaacc gtggccatgc cattgctccg ccttgctttt gatttcttcc tcgagggtaa
     1201 taagggagcc cccttcttga tccactgtat acaatcggtt ccttgcctga tggatgatag
     1261 accagttttc ccttttcgtt ctcgaagcgc cctcctcttg atactgtaaa caatccgaca
     1321 taatcaaaaa aacttaagcc tagacctctt aaaaagacat tttctttagg cggtatcttc
     1381 gaaagatcta catctgcagg attagaagga agctgataca gtaaaacatt tttctttgcg
     1441 tagtctaccc acactctttc ttcttctgtc tcatcatttt cccagtgacc gcttgctaaa
     1501 acaagctgat cgatagaata gctttgctga tctgtctgta aaagataacc atcttttctt
     1561 ttgcttactt tttttactaa agaataaaca aactgaatag aagctggaaa ttcttcttgc
     1621 aactttgaga aaaaccagcg ttgatagagt ccgtataaac atcgagtaga ttgctgatct
     1681 ttttccagac gattgatttc ttctataatt agttgcttat ttttggttac cgtctgttcg
     1741 atataatctg tagcaggtcc tttactccat tgaaacaagt tgggtccctg tgttaccaat
     1801 cctccactgg acaatgtttc atctgtaaaa agcgtcactt gttgagaaac agaattcatt
     1861 aataactccg gtggctgatt caaccgccaa actcgacctc caggaccatt gggatcgaaa
     1921 agaaaaagtt caagagaatg tctgtcctga ttattatgga gaatcctttc tgctacactt
     1981 aatcctctag gtccggcacc aatgatccct attttcattt gcatgcctcc atttcgataa
     2041 attttttcaa taaaacagca tggttctgat gttcattttt tgcaccataa acaaaagtaa
     2101 tgactggatg ttctttcata agttgactga gtttaaaaat agcttgcttc ttttcttctt
     2161 cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc aaatcgttct ggtacatggt
     2221 caaaagtttt gcgtgcttca ttactaggtg caatttcttt caaccacaga tctaactgcg
     2281 ctttttcttt tgaaattcct cgtggccaga atctatccac gagtattcta tagccatcct
     2341 ctttttcagc atcaaggtaa acacgtttta atttgatcat agttgtttcc ttctttctac
     2401 cttttcgctt agcttatata tgtagataat agcacaaaat aaaaaaaatg aacggcacaa
     2461 ctatccattg cgccgttcat gtgatcttgg tcaaaatcta atgattgatt cggtttttaa
     2521 agctgtcata caatcatctg ttttatactt ttttactttt gatttttcca gaccatccgt
     2581 catatccacc cttaagtaaa taaatctctt gataacctgc tttacgtaat ttatttgctg
     2641 tacggatgct caaacttttc ttttgatcgt aaatatagac aggctggtct tttcgaatag
     2701 atcctaatga atctttcaaa acagtgtaag ggatgtttct tgcacctaaa atatgcccag
     2761 catcaaacac atctttttcg cgaacatcga ttacttgtgc ttttctcatt ccttctcgaa
     2821 attcttcttc tgtcaaagtc gttgctgaac gtttggccat tactcgtaga tagacttccc
     2881 aaaggatgat tgcaagcaca atcaatagca aaatgatatt aattacccat aaaaccatgt
     2941 aatgcctccg tttttttaag caaatttcaa ggctaatgag gcagcaccaa tgacaccagc
     3001 ttcattacca agctctgcta atttgatttt tgttgattct cttacttgcg ggaaagtaaa
     3061 ttctttaaaa tatttctcta cacggctacg taaaaattct cctgctgccg atactcctcc
     3121 acctaacacg atacttgacg gatttaatgt attccccaag tttccgcaag ctaaacctaa
     3181 gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga tcgtcttctt gagccatttc
     3241 aaagatatct ttacttgtta cttcttctcc gttgtctagc attgctttca gttttgaatc
     3301 ccctgcgtat tcttctgcca agaaacgacc taggcgtacc acacctgtag cgctagatac
     3361 agtttctaaa catccgcgtt taccgcaagt acattcgaac ccttcaggat ccacagtgat
     3421 atgtccgatt tctcctccag ctccgctcac gccgtgaatc aaatttccgc cagcaacgat
     3481 acctccgcca acacctgttc ctagagtaac gaatacaacg tctggatcgt tttctccagc
     3541 acctttccat cgttcaccta aagcagcaac attcgcgtca ttatcgatag cgaatttgat
     3601 tcctgttcct gcctcgatca gttttttcac aaattgaact tctgtccagt ttaagttata
     3661 cgcaccaata accgttccag cttcgctgtc cacgcttcct ggggtaccca tgccgattcc
     3721 gataaagtct tcggctttca tattatataa attcaaacga tgattgattg attcaatgat
     3781 ttcaggaacg atatgcttac catcatccaa aatattggta tcgatgctcc atttttgctg
     3841 aatctctcct tctggtgtta aaattgcaaa ttttgctgtt gttccaccta aatcaatccc
     3901 aataatcttc ttgtccatta caaatttcct ttctttcggc tttcttcaat tcggtgctct
     3961 ctttttaata tgcgccacgc tgtcatgtat gtatcatgat caatcaaacg gctatcatac
     4021 agattacgca attcgatagt cattaattca atgtcataga tgcgtgcacc cacatagata
     4081 aagatgccaa attttttgaa cagctgctgt acatcatata agctctccat ctttaagcac
     4141 ttccttcata ttatacaagt aaaccatatt tttttaaacc cagtaaaata catattacaa
     4201 aaagaaatac aaaaactagt gtagaaagaa tcctttggtg tatcgaatag cttgtctgtt
     4261 gtttcggtag acccaaaaca ttccctaaca ctaatccgcc aattagccca ccgacatgtc
     4321 cccaaacatc tacagaggta tccagaagac caaaaacaaa tgtcaacaca ataaataaaa
     4381 gaaaatgtct gactagctgc tggatcgctg ggttatgttt aaaatgaaac cctaagataa
     4441 acaacgcacc gaacaaaccg aatatcgaag tacttgcacc accagacaga acattcgctt
     4501 cgttaaaagc aaagcttgct gtatttccca ttatcccgct gaacatataa atcaagaaaa
     4561 aacgccaatg gccatagatt gcctcgattt gctgccccat aaaatacagt atcagtgagt
     4621 tcattacgaa atgcatcagt ccaaaatgga tgaagattgg tgtcactaaa cgccaccatt
     4681 cattgaagtg aacaagataa ggaccataca ttccggcata ataagggatc tcaagtcgcg
     4741 gaaataattc catcatgagg aagaccgctg tctgtatccc taaaaaagca taggtccata
     4801 acggtcgtct taaccatgca cgcaatttta tttgctgttg ataattcatg aaaacctccc
     4861 atttcatttt agtcaataaa caattgttgg atcttctgat caaagctctc cggttgccat
     4921 tgttcattca attgttcacg aaaaacaagg ctacatgttt ttttatgaaa attatttaaa
     4981 taccggtcgt aataaccacc gccaaaaccg attcgataac cggctctatt gtagataata
     5041 cctggtacaa tgaggagatc gatcatctcc ggcaaataga gcttctctga ttgtggctca
     5101 agaacaccaa aactcgaacg tacaagaacg gactgttcat ccatttcata aaacgccatc
     5161 tgacgtttcg gcaaagtttt aggtacaact attttctttt gttctttttc tgctcgttgg
     5221 ataaccggct gtgtatccag ttcaaattca ttcgataaag tcgtccctat cacttttgct
     5281 gacttccagt actcgctggc gaatagctgt ttatagattc gctgttcttt ttcctttttc
     5341 ttgccacttt tggccagatt tttcagcttt tcgattcctt tccggcgtaa tcttactttc
     5401 aattggtcca aaaactcacc cttccttctc tcataaaatg gtataggaaa aaaagaaaaa
     5461 tagcaatcga actttcaaat aaatgcataa atagaataaa aagaagacct agaaatgatt
     5521 ggccttcctt ttttaatgaa tttcaactgt tcagcgcgtg tttttgtctg ataaaaagtg
     5581 catacagaaa atatggcgca ccaaaaaggg caatcaaaat tcctgtaggt acttccccac
     5641 ttagtagaat cactctagca accatatctg cactaagcag aaaaatggaa ccgaacaaac
     5701 cgctcagaac aaaggtccag ttattttctt tacgtacgac tagcttcgca gcatgaggag
     5761 ctagtagtcc tacaaaagat aaactacctg tcaccgctac actaccgctt gccaaagcaa
     5821 cagctaacaa caaaaggatt ttttgacttt tagccacgtt aaggcctaat ccttgcgcgc
     5881 gttcttgacc aaatgatagc aatacaatcc tctgtgcata aagtcctcct agcggaatta
     5941 agataaccgc ccatggaagc aatgcccaaa cataaggcca gctcgcaccc cagattgatc
     6001 ccgctaacca tgcattcaca aatccataat tttccttaga taattggatc gtgcatagca
     6061 atgttaatgc agaaaggccg gcattaacgg caataccgga caataagatt cgattcatac
     6121 ccaaaaagcc atttcgatca taggccgcaa gatagacaca gcaagcagct aaaacaccac
     6181 ctagaaaggc tacaactagc aatagaaacg gcagcgaata gtgcgaaaag aaacctaaat
     6241 aaagcatcac taaaaatcct gctccggcat taatgcccaa tatactagaa tctgccaatt
     6301 catttcttgt tactccttgc aagatataac cagaaacgcc aagacaagct cctgctaata
     6361 aactgatcag taatcgagga actcgaaatt ctctaacaat cagcgccaaa gaagatgatt
     6421 ctttccctat gaacgtggca aaaagctgat ccaggtaaat aaaaggcgta ccaatcatta
     6481 aactacctat agacaggatg acaagtacaa aaagcaagaa atagaacgtc caatatattt
     6541 ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac ggaacgccaa tcaatgcaat
     6601 gactaatcca aaaggtgttt caaaaggggg attgatcatc ctcgctgtaa aatccgccaa
     6661 cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga acataacgat agtccgttcc
     6721 aaccattccg cgaacaactg gcggaaccaa cagcccaata aatgaaacag gtccgactaa
     6781 cgaaacggca atggctgcta acactgcaac tagacagaaa acaatcaacc gcgttcgttt
     6841 aggatgactt ccaagagcta atgcatgtgt ttcactcaaa tgaatcaaag atattgcttt
     6901 tcctaatgaa aaagaaacca aaacagcaag tatatataaa ggaaacaata ctgctaattg
     6961 tttccatgtg atattggcag ctccgcctac aaaccaaaaa gcaagatcct gctgcagatt
     7021 aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa gaactaatag ctacgccagc
     7081 taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac tgcaaaaaac caaatacgag
     7141 caaagttaca gcaaaagaac ctgccaatga cattagaaac acagaaaaat cagaagcttt
     7201 aggccaaaaa gcaaaactaa gggccattgc caacgaagca cccgcgttga ttcctagtaa
     7261 gcctgaatct gctaaaggat ttcttgtcac tccttgcatc aatgcaccgc tcaatgcaaa
     7321 acaactccct acaagaaatg ctcccgccac acgaggcaaa cgaatatttc gtatgatttg
     7381 gtgactttgt tttgatctat caaaatcaaa caacgcctgc caaaaatctg cttttcccac
     7441 agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc aaaagaacaa aacagaccaa
     7501 aagcaggagt ctatttttct tcatagtcct cataattgat cctcctgctc ttttggcaac
     7561 aaatcatagg ttagaaaaac tggtttattt gttcttggaa gcgtgacaat cgttgcatca
     7621 atctcaaata tttttttcat acactctttt gtgatcactt cttcaggtgt accttctgtg
     7681 atcaacttac cgtttttcat tgctaataaa taatctgaaa aacgtgaagc atgattaatg
     7741 tcatgaatcg acatcaagat cgttgtttgt ttcatttgat tgatttcagt caataactgt
     7801 aggatctcta attggtgtgc cggatctaaa aatgttgtcg gttcatccaa gatcaagata
     7861 tctgtttcct gagcaagcgc catagcgatc cagacacgct gggtttgtcc accagacagc
     7921 gttgcgatat cccgcatacg aaatccagct agacctgtag catccaacgc ccactcaatt
     7981 gcttcatgat cttgcgtgtc catgccactg aacagttttt gataagggta tcgtccgtaa
     8041 gatactactt cttctactgt catacctgta gggtgttcac tagtttgtgc taataatgca
     8101 attttttttg caagttcttt tgaactgtat gtagagatat cagagttatt gacaatgatc
     8161 tttcctgatt tcacaggtag tatacgggta aaacttttca ataaagtaga ttttccactt
     8221 ccatttggcc caatcaaact agtgattttc ccttcaggaa tcgtaacaga catgttttca
     8281 atgatttttt tgttttcgta gccggctgca atgtcagctg ctcttaatct cgtcatttca
     8341 cccatccttt cgctgaaaat cattctcaat tatgacgctg tttcaacatt tcgtcaagcg
     8401 ctactttcat atctatagcc aaatgaagcg gttgacagcg ttcgaaatat caggaataat
     8461 aaccaatgag aaagattctc aattatgtta ggaggaaaga aatgaaatta aaaacaaaag
     8521 tattcacatt atctagcata ttgctgctgg ctggagtact gagcgcttgc tccccctctt
     8581 cagataaaga aacagctaca actgccaatt cgacgacttc atccaatagt actcatacag
     8641 tcacagatac acttggacat aaagtagaga ttccaaacgc gcccaaacgg atcatcggca
     8701 gttacttaga agactatctg attgcactag gggaaaaacc tatcgcccag tggactgttg
     8761 gcagtggttc cattcaagat tatctgcaag atgatttaaa ggatgtccca acgatttctt
     8821 atgaccttcc ttatgagaaa gtgttaagct ttgagcctga tttgctgttg atttcttctt
     8881 ccgctactgt cgaaggcggg aaatacgaac aatatagcaa aatcgcgcca acttatgttg
     8941 taaaaaacgg aacagatgtc acatgggaag aacaactaaa agatgtcggc aaagctttgg
     9001 caaaagaaaa agaggcagaa aaaatcattt ccgattatca aaaatccatc aagaaaactc
     9061 gagaagaact agcagataaa atcgagaaca agacagctgc agtgttgtgg gtgaccaaca
     9121 actcagcctt tatggtcagc aaaaatcggt caagtggaag aatcgtatac gacgatctgg
     9181 aatttggtgt tcctgatcta gtggaagagg tctccaaaaa agccacttct gactggtctg
     9241 ctgtttcctc tgaaaagcta gctgaattag atgctgacta catcatcctt gtgaacagtg
     9301 acaaagatgc tgccatgttc aatgaaccaa gctggaagaa tctaaaagca gtgacagaca
     9361 atcatgtact gcaattcgga cctgaatcta gttggctata taacggacct attgccagtc
     9421 aggaaatggt caatgatatc aaagaaaaac taaattgatt ttaactaaaa aaagcatctt
     9481 cacgattagt cgtgagatgc tttttttctt attaacaaac tagacgcata gattttattc
     9541 ttcttttgaa gcaaccctat tttttactct ttcccatttt tgaaaacgat gggcatatag
     9601 attttcttga tccgttatcc cctcatactc ctctataagg gcgaagtctg aaaaatcaat
     9661 attcccaata tacgtatcgc cttcaaacgt gctatcgatc aatgttcgcc agatccgacc
     9721 agtgtaagga agaagttctt gaaaaatccg actaccaccc gaaacataga tatcttctga
     9781 gatcgtttga ctatacgaca aaagttcatc gatcgaatgc attacttccg cattttcttt
     9841 cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg cgataaggaa gtgaaagatc
     9901 ccccattcct tcataggtct ttcttcccat cactaaaatt cgatccattg tgtgttcgcg
     9961 aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa ccatttttgc cgatcagacc
    10021 atttttatct tgtgcccaca tggaaataaa catattatcc gctccttatg attttagatg
    10081 ttttgagata aaaaaagtct caattctttg accagcaagg aattgagaac tttaaaaaca
    10141 ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt gggcaatatt tttgtttttc
    10201 caaacgatca ggattgttac gtttgttttt gcttgttaga tagttacgtt ctttacaaga
    10261 agtacactct aaagtgatgt ttacgcgcat gtttttccct cccattttta caagaatcct
    10321 ctaagatgac tctcagactt gattatctta ccatgatttt ttctagattg ctagtgtttt
    10381 tcacgatttt gttaagtaaa actacttgac gataaataca acggttttac ttctcaaaca
    10441 aaatcttttt ctttttgtat aaataccctt acacttgttt atatagatta tgttttttaa
    10501 taaaggggga tactaatatg ccatggaaca tgaaggactt tcctgcatca atgaaaaact
    10561 tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa tgcattatta gatgaagggt
    10621 accctgataa ccgtgcgatc ccaattgcca ttgatcaagc aaaaaaatgg tatgaagatg
    10681 ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa tccaagcaag acggatgaac
    10741 acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga tgtgatcgta gaatatgagg
    10801 aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc aagcaaccat tttgatacaa
    10861 aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa caaagaatca actttgttca
    10921 tttataaaaa agatgggaca aaagaaaaag aaatcgatta ttgaacatat gtaaaggggt
    10981 tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac aatggtgttc ctctgccaac
    11041 tccgaaccat aagtcataat atctataaaa cgggaagagc tgttttttga aattgtttct
    11101 tatcactcag tgttaaacgg taggagaaaa cctctttttt attgatattt gattttcatc
    11161 aaacagtcac atagtcgatt gcttctgctt gcggaatcgt ttcatctaca aagtagactt
    11221 tataccatgt caagaaactg aagattgtcc agatttttct ttgctcttct ttacgtcctt
    11281 cgtgatggtc atctagcaac ttcatgattt tttcttgatc aaaaaattct tttgcaaaat
    11341 ctgcttcgaa taatgctttg acttgttcgt agccgtgttc ttctttcaac caagctttga
    11401 ttggcacagg gaatcctaat ttcacacgat tcgaccattc ttccggaaga tgtttgttcg
    11461 cagcttgtct gaaaatatct tttgtgttgt tttgattcaa taaaaactta cttggtattt
    11521 gttgagccaa tttcatgact tcgatatcca ataaaggtac tcggacttcc aaagaacttg
    11581 ccatagaaag tttatctgcc tttaacaaga tatcttttgg catccattga tgaagatcga
    11641 catattgcat cttattgact tcatctttga ttccttctgt tttctcgtaa tgaacagtca
    11701 tgatttcatc cacagatggt gctgtttgat attttggtgt taacacttct gatgcttctg
    11761 attcttcgaa cactcgagca tgaccgataa aatattcttt tgcaggagca agtgattcat
    11821 ataaatggat ccgtccatgg aaatttttca tttttccgat tttacgtcct aaattatagc
    11881 gtgttccttt tggtaatttt ttcaatcctt gagcaatcac acggatgaat tttgaattcg
    11941 tatggaatcc atacgtttgg tatcccgcaa acagttcatc cgccccttct ccagattgga
    12001 ctacccgcac actttgtgaa gcaagatttg ctaaaaagta caaaggtacg caagaaggat
    12061 ttgaatcagg ttcatctaag tgatactgga tcaaagggaa tgctttgaac gacatgtctc
    12121 cgtcgatcac tgctgctgta ttatctaagt ccaatagttc ggttagtttt ctggcttcaa
    12181 tcgcttcatt ataggtttta tcatcaaacc cgattgagaa agaatgatct ggtttcaata
    12241 cagaggttac ataactggaa tcaaccccgc tggataagaa agaaccaact tctacatcac
    12301 tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc gattttttcg atccattctt
    12361 gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca atattgctga atgtctattt
    12421 ttccatcttt atacgtataa taatggcctt caggcaaacg atagacatct ttgaagaatg
    12481 tttcgccgtt taatggagaa tattggaaag tcatataagg tttcaacgct tctttgttca
    12541 attctttgtt aaaatcagga tgaggcagaa aacttttgat ttctgaacca aacataaatg
    12601 ttccgttcat ttctgcataa taatatggtt tgatcccaaa gtggtctctt gcaccgaata
    12661 actcattctt ttcattatcc caaatagcaa aagcaaacat tccgcggatc ttttgcaata
    12721 attctgttcc ccattcttcg taaccatgca aaaggacttc tgtatctgca tgtgtttgaa
    12781 aaacgtggcc agctgcaatc aattcttctc tcaaaggctg gtaattatag atttcgccat
    12841 taaaaataat gatctttgtc cgatcttcat tgtaaatagg ctgagtaccg ccctctaagt
    12901 caataatact taatcttctg aatcctaaag caacatgttt atcgatatat tctcctgaac
    12961 tattaggtcc acggtgaacg attcgatcca tcattgtatt aataattgtt tttttgttgt
    13021 ccttgtcgtt aacaaaacct acaataccgc acatttaact ttcacatcct aatataaaat
    13081 tactgtttat ttcattcata tgcatcttat catagaataa atagcggacg aatattttct
    13141 ctcttacaat ttcgtcacgt gaaaacaaaa gatgaaagtt tctgttttta cagaaaataa
    13201 agtcccaata tctaatttac catgttttct tttttgaggc taccttattc tcttcgtgtt
    13261 aaaaaaatat tagtggatgc tatttccaaa aaaaccagca aaaaaagaca cgacctaaag
    13321 atcgtgtcta attatgactg tatcagaaaa tatgatacca aacggatcta ttcattaatt
    13381 aattgacata gtttatctgt tgctctttta tgactggata actgaccaat tcatgacgat
    13441 ccaagtcttc tttattcata tttacagcag taatttgact gtcttcatac gtacgataat
    13501 aataaatccc tttatcaacg ttacagcaag aagaataaat cgtatattcg tattttcttt
    13561 ctccagtatc gcatactcct ttttgttgct cgactgagcc tagtatatga aaaaattgac
    13621 tgatactttc agattctgaa tcacctgatg cagcattcat tttagtaaat gtggctttta
    13681 caaatcgaga aacagaagag agatctccag gtaagcccaa tcctcccatc ccacgactat
    13741 aaacattcaa gctgatctgg tttgaaaaat gattttctgg tgttccattt gatagcgagc
    13801 gataattgtt cagattaaat aattgataat caaatggcgg attatttgtc aacacaccga
    13861 caggattatc atataaatga agtccatctt tcatactttc aattactatc gatttttctt
    13921 tatcagctaa taaccagtgt aaaggtgaca agggtagttt ttcactaaaa tcgatattga
    13981 ctagattgat agtagccaat aattctttag cttcgtctat ggttgagcat tgccctagta
    14041 tccatggaat aaactcaaat ggtgcaacgt tatcttttcc ttcttgtaat tctttataat
    14101 cagcattgcc aggaaaattc agtcctgcca tactcaaccc tttttcattt gtagcctcat
    14161 aatagagtgg atagttggat accccagctg ctattcctat cattgcataa tgtttatcta
    14221 aattttttac ctttctaaaa tcgaaacgat aatttcttgg agtaatagtg accacttcat
    14281 tataagacat ctcataatca aaattccttc caaaataatg gtctttcgtt gcataagtga
    14341 ttgcggtaca catcgtttcc cgtcctaaaa caaatcgttt ttaca
//
LOCUS       DADXTG010000061        14243 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.062, whole genome shotgun sequence.
ACCESSION   DADXTG010000061 DADXTG010000000
VERSION     DADXTG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.062"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(121..687)
                     /locus_tag="IWI57_001609"
     CDS             complement(121..687)
                     /locus_tag="IWI57_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBC2449755.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(687..1643)
                     /locus_tag="IWI57_001610"
     CDS             complement(687..1643)
                     /locus_tag="IWI57_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBC2449756.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1643..2299)
                     /locus_tag="IWI57_001611"
     CDS             complement(1643..2299)
                     /locus_tag="IWI57_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBC2449757.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2669..4687
                     /locus_tag="IWI57_001612"
     CDS             2669..4687
                     /locus_tag="IWI57_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2449758.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4757..4963
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5114..7528
                     /locus_tag="IWI57_001613"
     CDS             5114..7528
                     /locus_tag="IWI57_001613"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBC2449759.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7876..9429
                     /locus_tag="IWI57_001614"
     CDS             7876..9429
                     /locus_tag="IWI57_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBC2449760.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9810..11594)
                     /locus_tag="IWI57_001615"
     CDS             complement(9810..11594)
                     /locus_tag="IWI57_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449761.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11609..13330)
                     /locus_tag="IWI57_001616"
     CDS             complement(11609..13330)
                     /locus_tag="IWI57_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2449762.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13430..14191
                     /locus_tag="IWI57_001617"
     CDS             13430..14191
                     /locus_tag="IWI57_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2449763.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4613 a         2668 c         2836 g         4126 t
ORIGIN      
        1 ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga aatcaacgct
       61 ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg cgttttttat
      121 ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt tgataaaccg
      181 ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat caaccatatc
      241 cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc gtccgcgagg
      301 cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca tagtgatgat
      361 tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga gattttgttg
      421 ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga cacgttcttc
      481 taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct ggatatcaaa
      541 actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat cgtggccatt
      601 gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca gtaatgtatg
      661 gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac atcatatttt
      721 ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac ttcccatttt
      781 ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt caaacgatgg
      841 atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac atagtcagga
      901 cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt tttgcgcatc
      961 agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt gcgtctgaca
     1021 ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg cgtacaaaca
     1081 cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata atcatgtgct
     1141 cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc tacccataaa
     1201 tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag acaatccggt
     1261 cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc aaatcgttct
     1321 ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac gatgtattgt
     1381 tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg gattggaaga
     1441 gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa agtacaaccg
     1501 ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat cggcaccttg
     1561 aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata tcttttattt
     1621 ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta tctatttgtt
     1681 ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg agtaagtagc
     1741 cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc ccgacactta
     1801 ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt tgaacaaaag
     1861 ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca tgtccactag
     1921 ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct cgcatgaaaa
     1981 atagtttgat caccgggttg atgattccag caattacaat aaatccagaa acgaaccaaa
     2041 atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca aataaaatga
     2101 tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc agatcaggat
     2161 aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac gacagatgga
     2221 actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca gccacctgaa
     2281 atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta tagcattttt
     2341 ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct ttgaataacc
     2401 aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa tgcaaatgaa
     2461 atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac gtagacgtag
     2521 gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat gattacaaaa
     2581 aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa ttattaataa
     2641 tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag aacggagacg
     2701 tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg tgggaactgc
     2761 gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc aagcggatga
     2821 aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag aaattgctac
     2881 ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga tcgcacaagc
     2941 agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt ataatctttt
     3001 tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg aatttttaaa
     3061 taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg ctgaatcttt
     3121 caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt actatgccgg
     3181 tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga cagggcgtta
     3241 tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt acggattgac
     3301 ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag gtggcaataa
     3361 tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa actcaggttc
     3421 agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa tctcaaataa
     3481 attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca atttcattta
     3541 tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga cgaataattc
     3601 cggtaacaat accaactctt caggaaatgc tggtacatca aacggaggac aaacaactgg
     3661 tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg atcacggaat
     3721 tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt atccaggaca
     3781 acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg gctcaaatac
     3841 tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag cttcaggaac
     3901 gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat acggaattac
     3961 gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc caggacaaca
     4021 actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag gttcgtcaac
     4081 caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag gcacttatac
     4141 cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca cgatggatca
     4201 gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc aattgatcgt
     4261 taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag cgaaaccaaa
     4321 tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga tgtataccgt
     4381 gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga tggatcagtt
     4441 aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag tgattgtgaa
     4501 gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa cctatactgt
     4561 gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa tggctcaact
     4621 aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt tgattgtagc
     4681 taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat ttgttactat
     4741 aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg aagtttcaga
     4801 gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg agttgccctt
     4861 gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga ggccttttca
     4921 ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc aatcttttta
     4981 taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga aataaaaata
     5041 aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt tcaatagaac
     5101 aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc aaaaatactg
     5161 ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat tttacgcact
     5221 ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg aaggttatac
     5281 ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc tgcatccaat
     5341 ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag gaaacgatcc
     5401 agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt ctttaggatt
     5461 tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata aatggacaca
     5521 atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag tagcggtaaa
     5581 ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg ggaaaagtga
     5641 acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga aaatcactgc
     5701 ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa gcattaaaga
     5761 aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta aagtggctgg
     5821 tacggataaa tcctataccg tatttacgac acgaccagat actctattcg gtgcaactta
     5881 ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg aacaaaaagc
     5941 tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga gaacggatct
     6001 tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag ttaatggcaa
     6061 ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag gagccatcat
     6121 ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg gactagaaat
     6181 tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag atggtacgca
     6241 catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata aaatgaatga
     6301 atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac gtgactggct
     6361 attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg aggacggaac
     6421 agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa aaaacatcaa
     6481 gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca atgtcgttga
     6541 tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat gggcaggaag
     6601 ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag cagattatga
     6661 aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac atgcggtact
     6721 tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg ttgttcctac
     6781 aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa acaacgaaaa
     6841 aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa cttacggtgc
     6901 agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga ttgcttggag
     6961 cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt tgatcgttga
     7021 tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc ttgataaagt
     7081 gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt tcaatacagc
     7141 aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc tgccttatga
     7201 atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg gtgaagaact
     7261 atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa catacgatga
     7321 agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta aagttcgtgc
     7381 gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag cattagccaa
     7441 tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg cggtaccaaa
     7501 caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac tgatgagttg
     7561 tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt tgttatttca
     7621 aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac acgttcaatg
     7681 tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt ttgtctatta
     7741 atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc aaattcataa
     7801 attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa tgaagtatga
     7861 gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc agctaaacaa
     7921 aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct tttaagctta
     7981 gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg ccatatgagt
     8041 gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca cttttcgtgg
     8101 ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat ttggctccgt
     8161 taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca acagttttta
     8221 cataataaac caacttctca agatgatgac tatctttatt ttgagaagac ttcgacgagt
     8281 caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact tgatgattta
     8341 cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca tattttttta
     8401 ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata cctgacaact
     8461 aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac aatctattat
     8521 gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca catgacaatg
     8581 aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc tgatcctaag
     8641 caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac agagacgcac
     8701 tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat tgattcgcaa
     8761 tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg ttattggatg
     8821 gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc tggggatgat
     8881 gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat tatcacccaa
     8941 tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg tctatttatc
     9001 tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac tatggatggc
     9061 ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca aaatgtcagc
     9121 ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta tacaaacaac
     9181 ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca gacagggggg
     9241 atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac gttagagaca
     9301 aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc ctgcctcttc
     9361 cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca agtagaacaa
     9421 ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca agaaacaaat
     9481 tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga gttgacaaga
     9541 gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt gtcttattgc
     9601 cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca agtcttttgt
     9661 cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt attattcttt
     9721 gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga ctaatcatac
     9781 atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact attataaaga
     9841 ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc gacgatatcg
     9901 ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg gtgtgcaatc
     9961 acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa tgcttctgtt
    10021 cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt cgctagaatg
    10081 actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt ttcgctagaa
    10141 atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg tgcgatttca
    10201 gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat attatcagct
    10261 accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg acgtagttct
    10321 ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt attcagttca
    10381 taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg accaacgatc
    10441 gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat tttatctgat
    10501 gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc taaaagtctt
    10561 gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat cctatcgatt
    10621 gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat tggttcagaa
    10681 atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc acctgataga
    10741 acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat gaaacgaatg
    10801 gccggataga tagcacagtt cataaattga gcacgtttaa aagcgttgta ttgcttttta
    10861 tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt gaccactaga
    10921 ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata ctgattctgt
    10981 tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact gccaacgatc
    11041 agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag accagcagca
    11101 gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac ttgagaaagt
    11161 tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt gtcaaaaaac
    11221 gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa tgtgatcttt
    11281 tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct taagactgaa
    11341 cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact gaaagaaagc
    11401 tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat tcccatgatc
    11461 aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa tccgtaaaaa
    11521 tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt gttcaacgtt
    11581 tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca aactcctgat
    11641 aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt tttccttcat
    11701 ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca acaatcacga
    11761 gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct gttttatagt
    11821 ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc aacgctcgag
    11881 caatacatat ccgctgtttc tggccccctg aataattaga accgccttga gcaacaaagc
    11941 tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc ttgatcgctt
    12001 tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt aaaattgtcc
    12061 cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc tgttcttgag
    12121 tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg tcactaagtc
    12181 tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg atcgctgttg
    12241 tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag atttcagtat
    12301 atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt ggttcagcta
    12361 ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct tctatccgtc
    12421 gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc atcaagtaag
    12481 caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt aatgctcctt
    12541 ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc atgagcaaaa
    12601 aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga ttagcatagt
    12661 cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag gcacggatca
    12721 tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt tttggctgga
    12781 tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat acataaaaaa
    12841 caacagagat caatgtaacc catgttagta atagagaata acgaaacatc atgagaatgg
    12901 tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca atcatttgct
    12961 ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta aaacgactga
    13021 tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt tggaatccaa
    13081 acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt gttcctagaa
    13141 acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg ccacttgcta
    13201 tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg gcgatttcta
    13261 aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag aatgttttta
    13321 agatagacat attattcctc cttttttcct gatacttttc gattctatac cttcaagtaa
    13381 cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa tgaaaaaaat
    13441 gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga tccgctattg
    13501 ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc gattgtttga
    13561 tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag atataccgat
    13621 caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga cacttgatga
    13681 aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa aagagataag
    13741 ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat ttcctgtaga
    13801 agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg aaatcaaaaa
    13861 atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca gttcacagac
    13921 gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag acgtgatttg
    13981 ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga tgaaccatgc
    14041 ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat ggaaaacagg
    14101 agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca gaactgaata
    14161 ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata actcgaaaat
    14221 gatggttgtt ttagttataa aga
//
LOCUS       DADXTG010000062        14191 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.063, whole genome shotgun sequence.
ACCESSION   DADXTG010000062 DADXTG010000000
VERSION     DADXTG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.063"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(30..710)
                     /locus_tag="IWI57_001618"
     CDS             complement(30..710)
                     /locus_tag="IWI57_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449764.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(712..1785)
                     /locus_tag="IWI57_001619"
     CDS             complement(712..1785)
                     /locus_tag="IWI57_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2449765.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(1841..2079)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(2122..2355)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2432..3103)
                     /locus_tag="IWI57_001620"
     CDS             complement(2432..3103)
                     /locus_tag="IWI57_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2449766.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(3190..5868)
                     /gene="mgtA"
                     /locus_tag="IWI57_001621"
     CDS             complement(3190..5868)
                     /gene="mgtA"
                     /locus_tag="IWI57_001621"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBC2449767.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            6527..6964
                     /gene="ndk"
                     /locus_tag="IWI57_001622"
     CDS             6527..6964
                     /gene="ndk"
                     /locus_tag="IWI57_001622"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBC2449768.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(6986..7852)
                     /locus_tag="IWI57_001623"
     CDS             complement(6986..7852)
                     /locus_tag="IWI57_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2449769.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(8012..10105)
                     /locus_tag="IWI57_001624"
     CDS             complement(8012..10105)
                     /locus_tag="IWI57_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBC2449770.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(10854..11477)
                     /locus_tag="IWI57_001625"
     CDS             complement(10854..11477)
                     /locus_tag="IWI57_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449771.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(11636..13033)
                     /locus_tag="IWI57_001626"
     CDS             complement(11636..13033)
                     /locus_tag="IWI57_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2449772.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(13065..14036)
                     /locus_tag="IWI57_001627"
     CDS             complement(13065..14036)
                     /locus_tag="IWI57_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2449773.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
BASE COUNT         4034 a         3048 c         2369 g         4740 t
ORIGIN      
        1 taattttttt cattttttct cctccacttt caatgtgtaa tttttttaat cagttgatta
       61 ctgataaatt gacttaaaaa aactaagatt agaatcaata atgtagccac aatcgttaca
      121 tctgtttgga atcgattata ccctctcgat atagcaagat tccctaatcc gcctgcacct
      181 actgctccag ccatggtagt cagtccgatc acgttgatga tagtcaacgc cgagacccga
      241 atgataccag gcaaaccttc taacaaatac acacgtaaaa tgataccgat tggactggtt
      301 cccatcgctt ctgccgcttc gatcacccca ggatcaactt ctagcagtgc gttctcgatt
      361 tgacgagcaa aaaacggaac aactccaaaa atcaatggca ctaaagctgc tttcgcacca
      421 atcgtcgttc caactaagaa acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa
      481 gggatggaac gaatgatatt gatcagctta tcaagtattt caaatagcag acggttagct
      541 agtaatccat tagggcgtgt cactactaac gtcacaccta agatgattcc aatcatcccg
      601 ccaatgaatg ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc gacaaattca
      661 tccggaatca aataaacatt gggcaaatat gttttaaaga aatcagtcat tttatcttcc
      721 tccttcgatc actttgatac gactatctaa atcgtttttt tgtaaaatat ttatttttac
      781 accagatttg atcaaataat ccaatgcttt tttccgctgt ttggtttctc cagctaaagt
      841 aacgatcaaa ctccctaacg gcgtttcttg taataactct acattgccat ataaaatatt
      901 cgctgttact tgatatttgc tgtacagatg agcgattaga ggttcgttcg tctgatttcc
      961 aatataactt aattccacca gccatttatt ggcgatctga tcggcaaaag cagaatgggc
     1021 aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa
     1081 ttcttcttca ggattgctga agatctggat gctgctacct ttttcaacga ttcttccatc
     1141 ttccataacc gctaccttgt tgcagatttc tttgacaact tgcatctcat gagtgatcag
     1201 gacgattgtt aaacctaact ggcgattcaa tttttttaac aatgcaagga tctgaagggt
     1261 cgttttcgga tcaagtgcac tggttgcttc atcacataat aataccttag gttcattcgc
     1321 caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt taggataagc
     1381 agatgctttt tcttccaaac cgacaagttc cagtagttca ttcacttttt gacgacgttc
     1441 ctgtttcgat tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct
     1501 ttccattaga ttgaaatgct gaaagatcat accgatggcc tttctttctt ttcgcaattc
     1561 tttgggagag agctgactca gattcgtctg attgacaatg acttctcctg ctgttggttt
     1621 ctgcagcaaa ttcatcacac gaactaacgt acttttccct gcacctgagt aaccgacgat
     1681 tccataaaca tcgccttttt caatcgttaa atctacagaa tctactgcct gtatctcttt
     1741 ttctttttgt ttgaaacata cagaaacatc tcgcaattca atcattaaaa ttcctcctta
     1801 tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg
     1861 tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt acgatcatac
     1921 tctagcaaga tatcggtcgc agccgtcatc gccttacatc tatgctcggg atgaattgct
     1981 cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca
     2041 aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaagtccttt
     2101 tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa
     2161 gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg
     2221 aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt
     2281 cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac ttactttcct
     2341 tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa ctgaaaagtc
     2401 aagtacgaaa ttcaaattat tttttctata tttatttttc tgttttttta tttaacattt
     2461 caataacagt tcgaagatct tttccttccg caagaagttt caatttttcc ttttcttcga
     2521 cggtataatt tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg
     2581 aagcagctgt tgctcctact aaagaatctt ccactgcaaa ggctgaacta gcttctaatc
     2641 ctgttcgttc caaagctgtc agataaggaa tcggtgaagg cttgttctcc gacacttcgt
     2701 ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag atatctgttg
     2761 ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat atgacgttct tttaagtctg
     2821 ccaatatttc ttttgcatat ggttttaagt ggatggctcc gcttgtcagc tgttgataaa
     2881 aatattcttc acgcttcgct cggacttcct gtactttttc agggctacct gctgctttca
     2941 ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttcttttg
     3001 ggatatggat atcaaattgc tgaaatcccc aaagccagcc atcacgatac gttttttctg
     3061 tgtcaactaa caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt
     3121 ccaaaacaat acacatagtt tagcacgact ggtccaaaaa gaacagaagc aaagaaccag
     3181 ccttctcgtt tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac
     3241 aagcgccaga taagcgatga ctgtagcaaa taaccataac cagtaattgc caggaagtgg
     3301 catcaagcct aattcagcac caaaccttgt aaaaggaagg acagaaccga caataattcc
     3361 taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt tggataaatg gtactttcgg
     3421 tgtacgcagt gcatggagca ctaaggtttg tgtccatagt gattcgacaa accatcctgc
     3481 atggaataat gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga aacttccgcc
     3541 cactacagca ggacaaataa caaaatacat caacaagtaa gtagtaatat caaatatcga
     3601 acttgttgga ccaaagtaaa ccatgaattc cccaatacta gatgcattcc atttttttgg
     3661 ctcgtccaaa tattctttat ccatccgatc ccatggcaag gaaatgcagg aaacatcata
     3721 gatcagattc aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca ggaacgtact
     3781 tgcaatcagc acggaaaaca tattgccaaa attagaactt gctgtagcct tgatatattt
     3841 catgatattc ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc
     3901 tttttctaaa aggatcacgt ctgcagattc tttcgcaata tccacagctg tatctacaga
     3961 aataccgata tcagccactt tcattgcagg agcgtcattg ataccatctc ctaaaaatcc
     4021 gacagtatga ccatttttac gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa
     4081 ttttacgaat acttcatggt cttcagctac ttgtgctaat tcatgatcac tcatttccga
     4141 gattttctca ccggtaacca attcattgat ctctagacct acttgacggc acactgaacg
     4201 tgtaactaat tcgttgtccc cagtcagaac ttttacagct acaccatgtt ctttcagagc
     4261 tttcaacgct tgtttcgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcaa
     4321 aaccatgtct gactcatctt tgactgaaaa ttctccaacg acgcttggat tggtcttttg
     4381 tgcgacacca atcactcgta agccatcttc attaagtgac tgtacttttg caagtacggc
     4441 ttcttttcgt tcttttgtta atggtgtaac agtaccgcca agatcaataa atttagaaat
     4501 gttcagcatt tcttcaatcg cgccttttgt gatcatttgt gttttaccag ctggatcagc
     4561 aaccacaaca ctcatacgac gacgttcaaa atcaaaagga atttcatcca ctttttgata
     4621 attgatctta tctgtatcta aagtttctgt tgcttcatca atgatagcca catctaacaa
     4681 atttttcagc cctgtttgat agtagctatt tagatacgca tggcgcaaaa cacgatcatc
     4741 ttcatttcct tcacaatcca tatgatattc aagaatgatt ttatcttgtg tcaatgtccc
     4801 cgttttatcc gtacataaca catcgattgc accaaagttc tggatggcat tcaaattttt
     4861 gatcactgtt ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat
     4921 cattggaagc atttcaggtg ttagaccaac tgctacagat agtccaaaaa gaaaagcttc
     4981 tagccaatcc ccttttgtaa gcccattgat gacgataact gctggagcca tcaatgacat
     5041 aaatttgatc agtagcatgg atgttttatg gatgcctaat tcaaagcttg attgaattgg
     5101 tttttcagat agcgtttctg ctacatgacc aaatagtgtt tcatttccaa cagaaacaat
     5161 gattccttcc gcacttccac tgatgacatt acttcccata aagacaagat tttcgtaact
     5221 tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc
     5281 ccctgtcaat gctgcttggg aaatgaataa atcctttgcg gacaataaac gcatatccgc
     5341 tggtatcata tcccctgctg aaagccgaac aagatctcca cagacgattt cttccatagg
     5401 aatctctgtg tactttccat tacgttttac agctgccgtt actttcacaa gtgatttcag
     5461 tttttcagcc gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat
     5521 aaccatagtg aagataatgg ctacaccgaa aagatcttta tctccaggag ctgctaatac
     5581 atattccgtg atgaatgaaa taattcctaa tgcgatcaac actaatgtaa atggcgtgat
     5641 gtatgctttc aatatttcta caatgaatgg tgtttttttg ccatatgaaa tcgcattttc
     5701 accgtaattt tctcttgaac gttcgatttg ttcttctgtc aatccatttg ctgatgcatg
     5761 gaatagttca aataattctt gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt
     5821 atctttcttt ttttccatct caatctctcc tttgtgtcca aaagacagct gttctcgctg
     5881 cttttggtct ttagctattc atcggatcga tagagaaaaa actgaaaggg gctgagaaca
     5941 gatagataat agccctggcc tttcctctag atccttgttt ttcgaatgac tgtcatccat
     6001 ttgttctcat ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata
     6061 gttatttttc caattagacc acctcctttt tatttttcac cgatggggat ttgcggcaac
     6121 aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcatatta aaaaaagctt
     6181 ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata cctgctttta
     6241 gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc gtctctcgac
     6301 gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatct tattcggttt
     6361 tccttcgtta atataacaac taaaaaagag gatgtcaaat caattttcca tgtttttcat
     6421 acttttttta aaatacacac gttatattcc tgtctcacgc ggttttattt gtctcatttt
     6481 ccacctcccg ctataataga aatatagtag gaaacgaggt gctgttatgg aaagaacact
     6541 tgttattatc aagccagacg gcgtgagacg ccatttagtc ggaagcatca tccaacgttt
     6601 tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa actatgactc ctgaattagc
     6661 aaaagaacat tatcagcact tgactgagcg cagttttttt gatgaactga tcgattacat
     6721 gacttccggt cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg
     6781 taagatggtt ggagcaacaa aagctgccga tgctgttcca ggtacgatcc gaggagacta
     6841 cgcgctgccg gggactgaaa acatcattca cgcttctgat tcccgtgatg ctgcggtaaa
     6901 agagatcgcc cgctttttcc cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa
     6961 gtgatacaaa ttctaaactg ctggtctagt tgctgggttc agcaatggcc agcagttttt
     7021 tctctaagga cctccgttga ttctttccta acatatattc gagcactgtg aagaattctt
     7081 ggtagtcttt cgtcttttct tcccctaaag cagcagaaat caatctttga taataatctg
     7141 ccatcaatgt ttcatagatc actgtacttc tttctttatg tgctaagctc cgtttcatat
     7201 cttcattcac actacgtgcc tttttcaacc gaccattttc tatataaaac gaagtcaggt
     7261 tcattaaaaa agtaaagaga tactgctcat gattgttcaa ctgtgctaat cgtcgcattc
     7321 tttttttgat tttcaaataa ttgttctcaa tgaaccgatc actgaatatc caaggaaaat
     7381 cagctagaat agatagttca aattgaccat agtctggtat ctgcatcaag tattcttcaa
     7441 tgattcggca ttcataacgt aaaaattcaa attctgtctc agatacttct gttttttcga
     7501 ccatgtgaag attcaaaacc aaataataca gatcttctgt tttcttaaaa tgcctacggt
     7561 tattgctgat ctctttataa ggatcaaagc tgtcttcttg tacccgatct agcattcgtc
     7621 tttttttctt taactttatt ggttcatcca gctggtttcg catccattca tattctgcaa
     7681 gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac aaatgacatt tttttcactg
     7741 aatttgtctc aatcccgact agagaagtct taaaacagat tccttccgct aattgggact
     7801 gtgtcaactt cttgttgact cgaagtttct tgatcaattc accatcatac ataatagttc
     7861 tcctttttaa tttaaatttt tccacattca ataaatagat acgttaaaaa ggaaaaatac
     7921 acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc aaccttcaag cattagaatg
     7981 ttgtctaact actgaaggtt ggtttgattg tttattgatt atttttttgc tgttcaacgt
     8041 ctgttcttgt tactggatat aagttggttg ttgttttatt accatttttt gaaaagacac
     8101 ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt acgttccgct tcatttttat
     8161 agtcatattt tttcggatca atcgtctcaa atccgtttgg cacataaaac cgcaagagat
     8221 tttcctgatt cagcttatct gaaagactta acgcagtctt gactttttcc tgatctttgg
     8281 agatttcctc atcgattcct gctgctttcg tatcgatgat ttctcctgtc tgattattgt
     8341 aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa
     8401 ctacttgatc atgctgagaa gataataagt ctgtaccaaa atgaagataa tctttatcat
     8461 cgattcccaa tagatgtagt aatgtcggca aaacatcgat ttctccaccg tattcatgat
     8521 tgactgtccc tttagtataa ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg
     8581 cgttgtcaaa ttcatcccat gtatctggat ctttaccaag agcgcttgct aaatctttat
     8641 tatctgtatt cgagatccca aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa
     8701 taccagattt tttcaaatac gtaaaaaatt gttccaatga ttgatccaga taatgtgccg
     8761 ttcgaacata attatctact gtgctattgc cagtattcaa agatggaaag tcgaaattct
     8821 tgtcatccgt ataataaggc gtgtggttgg ttacggaaag aaatttcgtg taaaacggct
     8881 gttgtagatg ctcaagatat tgggcagatt ctctgaataa atctttatct aatatcccat
     8941 aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc aaagaaattg tcataaccta
     9001 agtttttata tacatgatct ctattccaaa aactagctac attcccatgg aaaacagcgc
     9061 ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc
     9121 caagttctgt gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatatttt
     9181 cagcatcact tgtctttccc tgtcctactt catgaaagaa attgtcaaag ctgatcgttg
     9241 cttgattctg gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct accttcatat
     9301 taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat cacatttttc cctttagcga
     9361 tcccaaatgt ttcgggattt ggctctgcat aatgtttctt tgtataatct aaaacttcat
     9421 caatcccatt actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt
     9481 cataaatagt atacgcatcg attcctagat atttgacaat atatgaacgg tcaaatgttc
     9541 gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa aaaataagcc
     9601 ctgaaagaca ggtaacagcg atagccatcg gtttgccgac agggttgctt ttgataggta
     9661 ttttcttgac cttcaaccaa accagtagcc caatgaacac agcgatatcc agccagtaga
     9721 tgacatcatg gggcttcatc aaagaaaagg agcttcctga caatccttgc gacactttcg
     9781 aaaagcctaa tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatat
     9841 tcaagtacaa caaaagcgta tttaaaatat caatgatcaa aagaatagct aacgctggct
     9901 ttggcttttt aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca attgggttga
     9961 tccacaagat aaagtactgg atcgtacctt ccacacctaa tgaaaaatct gcatagtagg
    10021 caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt
    10081 ggcacaattt tttaagttgt gtcacgtata tcacttctct ctattcacat tttttcttat
    10141 tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt tttttattct
    10201 ttctaaagat tattccgtcc acttttcaca gcataaaacc gctgcgatgt aagcgattaa
    10261 actaatgaag agataaaagt cttgtggtga ccgcttcact ccacggtatt caaaagccag
    10321 tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat tcttgtcgca gctccttatt
    10381 tcttgatgca ggacagcttt ttcacaactt cttccttaaa cggtgttctc ctgccaactc
    10441 ctcggccaca agtcacaata tttataaaac atgagaagct gttttttgaa attgttcctt
    10501 gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc aatcagctga
    10561 ccctcggtat tagattatgt caggacgcta attttacaac ctcttggtaa acggtgttca
    10621 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atataaggag ctattttttg
    10681 aaattgctcc ttgtggctca gagttaaacg gcaggctcaa gacctcttga tacacggtgt
    10741 tcaatcagct gaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
    10801 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctctgt ttttcattgt
    10861 ccataggatt gatattgtcc ttgtgatgac tcattcgtaa taattgaggc ttgattttct
    10921 tcacttcccg ttatcgtttg tccactgcca gaatctgaag gacttgttcc tgtcgactcg
    10981 tttttttgag aagagacagt tgttttcttg acttgttcat ttgtcttttt ccattccgct
    11041 aaacgatcgg cgaccacttt tttgtctact ttttcagaat aagcggtgat cacttttcca
    11101 tttttcaaac gggctacttt gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg
    11161 tctttaaatt gatattgtct gagagaaatc gcactaagta cgtcatcgat attaataaat
    11221 tgattttctt cttttccttt tggcaaataa atgtccaatt cgcctttttc gtaattggca
    11281 gttattttat actccgccca ccaataagtt gagaattctt ttttgaaatt atctactgat
    11341 tctttgcttg ctacgatttt ttctgtttct ttctgagcaa gatctgctgt catttttcct
    11401 gaaaatgctg cggataaaat aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac
    11461 ggtctttttt tcctcatgct ttcctccact cctatttaat tttgactatt ctattatata
    11521 caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc gtaaataatt gaacttcttt
    11581 ttcacaaaat ggtaagaatg gagaatttca cgcatttgct ctcaactatt ctttcttaag
    11641 cctcaaatcc gtttttatct gatacgtctt tgaaccaatg acccgatttt ttgattgttc
    11701 tttttccatc ttgtgctaag tctactgaaa taaagccata tcgattctta tatgcattgg
    11761 tccacgacca gttatccata catgtccaca tatgatagcc taaacaattg gcaccttctt
    11821 gaatggcttg atggacatat ttcagatgag attggacaaa ttcgatccga taatcatcgt
    11881 ggatcatgcc atcttctttg ataaatcgtg cttcgttttc tacacccata ccgttttcag
    11941 aaataaagca gtcgatattt ccataatgat ctcgtacatt gatcaaaata tcatagattc
    12001 cttgttcata gatctcccat ccgcgataag gattcatttt acgaccaggc atttcataat
    12061 tgtcgaaata atcctcaggc attggaccgt ttttagtatc gattggtgtt tcttttgctt
    12121 ttacccggcg tggctggtaa taattgatac ctaaaagatc tactgtattt tctttgataa
    12181 ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag ttcttttact atctcaatca
    12241 attcacttgg aaactcgcct tttacagctg gatcaaggaa tgaacgattg aagaacgcat
    12301 ctgctatttg cgctgcttta acatcttcag ggtttgattc gtctcttgga tagcttggtg
    12361 tcagattcaa gatgatacca ataacaccac cttgctccat ttcatggtaa gatttgatgg
    12421 ctaacgctga agccaacgct tcatgatagc caacttggat cgcttttttc atatctgact
    12481 cttctggata atgccatcga tataaataac ctgcttcgac tggaacgatt ggttcattat
    12541 gagtaaacca tttttttact ttcgaaccaa acaattcaaa acaacttttg gcatattttt
    12601 cataggcttc aaccgtttct cggttcaacc atccgccttt ttcttgcaga tacatcggca
    12661 tatcgaaatg atacagattc acaaagggtt cgataccgtt ttcaatcaac tcatcaaaat
    12721 aacgatgata gaaacgaacg gcttcttcgt tgattgttcc agattcatca ggtatcaaac
    12781 ggctccattg gatagatgtt cgaaatgaat tatgcccagt ctgtttcatc aactgtacat
    12841 cctcttgata cttcgtataa acttgcgagg ttttttcagg tcctacttga tcaaagaact
    12901 tttgcggttc ttttttgtac caataatcaa aaatgttctc tccttttcca tcgcctgaaa
    12961 tccttccttc tgtttgtggg ccacttgctg cagaacccca ccaaaaattt gctggaaatt
    13021 gatatttttt catcaaagca cctctttttc ttttttagtt cgttttagtt gattccatgt
    13081 atgatatttt ccatcacaat cactttttca ttttcttcct cttctttttt gattcgtttt
    13141 aacagctgtt ctgctgctcg ttccccgatt ttatctgttg gtggcgtgat cgtgattttc
    13201 ggaaggatac tcagatcgtt tttatctaaa taatcatagt gactgaacgc aatatccttc
    13261 ccgattctga caccttcttg attgaaacat tcgatggcac caatcgacat attgtaattg
    13321 ctgacaaaaa tagcagttgc ccctttttct aataatgctt ttgccccgta atagccgctt
    13381 tttctagaat agtctcctgt aaagacctct aattggatcg atggatcatt ttggaccgct
    13441 tctttggctc ctgctaaacg ttctcttgct acgtagtctg tttgagtggc agcaataaat
    13501 ccgatttttt tatgtccttg ttcaccatga tgagcgatca cttttgctac actttctcga
    13561 tcattcacta atattgtatc aatacctgct ggtccttctg gacaattcaa tgcgataact
    13621 ggaatactca tttttgtgag catttcaaat ccagaccact cttcttcgga taagaagaca
    13681 atcagcccat cgatcacatg cttcatcagg tattcaattt tttgttcaat tttttctggc
    13741 tgatcggcaa acccgcttaa taataagcta tacccatttt cttcagctac tctttcgatc
    13801 cctgagacaa cttccgaccc aaaacggctg gaaatcccgt ttatcaaaag tccaatagag
    13861 aagcttcgat tattcttcaa ccctttagca atgatgtttt ctttatagtc caacgctgaa
    13921 atcgcttctg ctattttttt catgttcttt tccttcagtt gctgaccatt caagtaacgg
    13981 gaaactgttc ccactgaaac accagccatc ttagccacat cacggatcga tgccatacat
    14041 tctcactcct gccagttaca tttgatttct ctttcttgtt gttcgattat gatgaccgca
    14101 ttctttgcat agtgttgctt gtcatcaata tccatataac taaagtcttc agtagagcaa
    14161 cttcctctca ctattaatta tacttaaaca a
//
LOCUS       DADXTG010000063        13875 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.064, whole genome shotgun sequence.
ACCESSION   DADXTG010000063 DADXTG010000000
VERSION     DADXTG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.064"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWI57_001628"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWI57_001628"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBC2449774.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWI57_001629"
     CDS             complement(2260..2511)
                     /locus_tag="IWI57_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBC2449775.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2657..3280
                     /gene="lexA"
                     /locus_tag="IWI57_001630"
     CDS             2657..3280
                     /gene="lexA"
                     /locus_tag="IWI57_001630"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBC2449776.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3460..3678
                     /locus_tag="IWI57_001631"
     CDS             3460..3678
                     /locus_tag="IWI57_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449777.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3967..4479)
                     /locus_tag="IWI57_001632"
     CDS             complement(3967..4479)
                     /locus_tag="IWI57_001632"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBC2449778.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4493..6790)
                     /gene="recJ"
                     /locus_tag="IWI57_001633"
     CDS             complement(4493..6790)
                     /gene="recJ"
                     /locus_tag="IWI57_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBC2449779.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6932..7375)
                     /locus_tag="IWI57_001634"
     CDS             complement(6932..7375)
                     /locus_tag="IWI57_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBC2449780.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7376..8164)
                     /locus_tag="IWI57_001635"
     CDS             complement(7376..8164)
                     /locus_tag="IWI57_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2449781.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8161..9102)
                     /locus_tag="IWI57_001636"
     CDS             complement(8161..9102)
                     /locus_tag="IWI57_001636"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBC2449782.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9279..9866)
                     /locus_tag="IWI57_001637"
     CDS             complement(9279..9866)
                     /locus_tag="IWI57_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBC2449783.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10087..11394)
                     /gene="obgE"
                     /locus_tag="IWI57_001638"
     CDS             complement(10087..11394)
                     /gene="obgE"
                     /locus_tag="IWI57_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBC2449784.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11688..12698
                     /gene="gap"
                     /locus_tag="IWI57_001639"
     CDS             11688..12698
                     /gene="gap"
                     /locus_tag="IWI57_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBC2449785.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12783..13718)
                     /locus_tag="IWI57_001640"
     CDS             complement(12783..13718)
                     /locus_tag="IWI57_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2449786.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3835 a         3106 c         2446 g         4488 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aacaactaga taaactaaat
     2581 aaaatacgaa cattcgtttg atttagcgct ttttatatgg tactatttat ttatcaagtg
     2641 aaagaaggtg taccttgtgg cccgacaaac tgaatcaaga caaatcgaag tcttacgata
     2701 tatccatgaa caagtaaccc aaaaaggata cccgccgact gtccgtgaaa tcggggaagc
     2761 tgttcagctt tcttctactt ctactgttca cggtcatctt gcacggcttg aaaaaaaagg
     2821 gtttatccaa agagatccga cgaaacctcg tgcaatcgag ttgacaaaac aaggtttaga
     2881 aaagattggg attcgtccaa caactattcc agtacttggc gtcgtaacag ctggagaacc
     2941 cattttagca gtagaggatg cttcagactt ctttccaatc cctcctaatc tttcttccga
     3001 agaaggcagt ctctttatgc tgacaattcg tggagatagt atgatcaatg ctgggatatt
     3061 ggatggtgac aacgtgatcg ttcggaagca agaaactgct caaaatggcg atattgtcat
     3121 tgcaatgact gccgaggacg aagcgacttg caaacgattc tataaagaaa aaaacttttt
     3181 ccgtctgcag cctgaaaacg acttgctcga accaatcatc ttagatcaag tatctatatt
     3241 aggacgagta gtaggacttt atcgtagtca tatctattaa ttgctactaa ttaaaagaaa
     3301 actttttaaa aagagtttga gacgttggtc tcaaactctt ttttcgcccg ggttcttctt
     3361 ctttcaaatt tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat
     3421 acgaacaatt gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt
     3481 ggcttagtat taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca
     3541 gctttacttg tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag
     3601 taccgtcaat ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac
     3661 agatccaggc aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac
     3721 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
     3781 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
     3841 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
     3901 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
     3961 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
     4021 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
     4081 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
     4141 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
     4201 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
     4261 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
     4321 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
     4381 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
     4441 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
     4501 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
     4561 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
     4621 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
     4681 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     4741 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     4801 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     4861 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     4921 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     4981 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     5041 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     5101 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     5161 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     5221 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     5281 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     5341 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     5401 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     5461 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     5521 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     5581 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     5641 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     5701 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     5761 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     5821 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     5881 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     5941 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     6001 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     6061 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     6121 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     6181 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     6241 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     6301 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     6361 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     6421 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     6481 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     6541 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     6601 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     6661 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     6721 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     6781 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     6841 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     6901 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     6961 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     7021 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     7081 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     7141 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     7201 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     7261 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     7321 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     7381 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     7441 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     7501 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     7561 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     7621 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     7681 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     7741 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     7801 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     7861 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     7921 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     7981 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     8041 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     8101 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     8161 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     8221 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     8281 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     8341 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     8401 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     8461 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     8521 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     8581 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     8641 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     8701 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     8761 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     8821 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     8881 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     8941 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     9001 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     9061 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     9121 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     9181 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     9241 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     9301 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     9361 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     9421 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     9481 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     9541 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     9601 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     9661 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     9721 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     9781 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     9841 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     9901 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     9961 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
    10021 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
    10081 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
    10141 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
    10201 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
    10261 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
    10321 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
    10381 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
    10441 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
    10501 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
    10561 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
    10621 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
    10681 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
    10741 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
    10801 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
    10861 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
    10921 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
    10981 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
    11041 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
    11101 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
    11161 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
    11221 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
    11281 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
    11341 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
    11401 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
    11461 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
    11521 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
    11581 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
    11641 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
    11701 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
    11761 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
    11821 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
    11881 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
    11941 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
    12001 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
    12061 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
    12121 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
    12181 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
    12241 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
    12301 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
    12361 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
    12421 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
    12481 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
    12541 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
    12601 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
    12661 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
    12721 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
    12781 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
    12841 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
    12901 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
    12961 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
    13021 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
    13081 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
    13141 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
    13201 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
    13261 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
    13321 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
    13381 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
    13441 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
    13501 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
    13561 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
    13621 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
    13681 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    13741 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    13801 tataacccgc gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca
    13861 aattgtattt ccgac
//
LOCUS       DADXTG010000064        13754 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.065, whole genome shotgun sequence.
ACCESSION   DADXTG010000064 DADXTG010000000
VERSION     DADXTG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.065"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..863
                     /locus_tag="IWI57_001641"
     CDS             <1..863
                     /locus_tag="IWI57_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2449787.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="IWI57_001642"
     CDS             1189..2040
                     /locus_tag="IWI57_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2449788.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="IWI57_001643"
     CDS             2137..4017
                     /locus_tag="IWI57_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2449789.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="IWI57_001644"
     CDS             complement(4057..4959)
                     /locus_tag="IWI57_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBC2449790.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5190..6593
                     /locus_tag="IWI57_001645"
     CDS             5190..6593
                     /locus_tag="IWI57_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBC2449791.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6593..8026
                     /locus_tag="IWI57_001646"
     CDS             6593..8026
                     /locus_tag="IWI57_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBC2449792.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8171..9097
                     /gene="trxB"
                     /locus_tag="IWI57_001647"
     CDS             8171..9097
                     /gene="trxB"
                     /locus_tag="IWI57_001647"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBC2449793.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9311..11038
                     /locus_tag="IWI57_001648"
     CDS             9311..11038
                     /locus_tag="IWI57_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBC2449794.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11053..11403
                     /locus_tag="IWI57_001649"
     CDS             11053..11403
                     /locus_tag="IWI57_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBC2449795.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(11495..12355)
                     /locus_tag="IWI57_001650"
     CDS             complement(11495..12355)
                     /locus_tag="IWI57_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBC2449796.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12447..12875
                     /locus_tag="IWI57_001651"
     CDS             12447..12875
                     /locus_tag="IWI57_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBC2449797.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13037..13213
                     /locus_tag="IWI57_001652"
     CDS             13037..13213
                     /locus_tag="IWI57_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBC2449798.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13240..13686
                     /locus_tag="IWI57_001653"
     CDS             13240..13686
                     /locus_tag="IWI57_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBC2449799.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4503 a         2350 c         3074 g         3827 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata ctatataact
     5041 taaaatagac tttttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg
     5101 taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatg
     5161 aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
     5221 gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga tatgaggaat
     5281 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
     5341 ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
     5401 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
     5461 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
     5521 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggtcttgg
     5581 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
     5641 caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat
     5701 tatgcaatgt gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag
     5761 aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata
     5821 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
     5881 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaatatga
     5941 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
     6001 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat
     6061 ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg
     6121 gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa
     6181 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg
     6241 tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc
     6301 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaatta ctgacgatcg
     6361 aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc aatccgctta
     6421 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt
     6481 atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc gaagttcaag
     6541 atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt
     6601 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga
     6661 tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc
     6721 tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa
     6781 aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg
     6841 gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgaaaa
     6901 agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca
     6961 cggaatcgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga
     7021 atacggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat
     7081 cttcaatcga tataaaggac ttgtcaaata ctggctgaca ttcaatgaga tcaatatgat
     7141 ccttcatgca ccattcatgg gagcaggact gtactttgaa gaaggagaaa acgaagaaca
     7201 agtcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
     7261 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
     7321 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
     7381 cttcattgat gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg
     7441 aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt
     7501 tgattttatc tctttttctt attattcttc ccgagtttcg actaccgaca acgaactctt
     7561 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga
     7621 atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
     7681 ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga
     7741 gaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat
     7801 gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat
     7861 tgatttagta tcagctggta ccggtgagat gaagaaaaga tacggattca tttatgtcga
     7921 tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta
     7981 taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa
     8041 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
     8101 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
     8161 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
     8221 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg
     8281 ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc
     8341 cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg
     8401 aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag
     8461 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
     8521 tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt
     8581 tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat
     8641 ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc
     8701 acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac
     8761 agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
     8821 caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga
     8881 tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac
     8941 agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa
     9001 agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta
     9061 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt agaagaacgg
     9121 gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga
     9181 acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc
     9241 atactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
     9301 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
     9361 agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc
     9421 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
     9481 caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga
     9541 tacacaaggt gaagaagcaa aacgccgagg tgtcgctatt gcttatgact cacgccatca
     9601 gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata tcccgtctta
     9661 tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac
     9721 gtttgcggga atcatggtaa ctgcatctca caacccagcg gcttacaatg gatataaagt
     9781 ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
     9841 ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag
     9901 cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc
     9961 agtgacgatt gatcgtgaat tggtagaaac aatggggaaa gatttgaaac ttgtgtacac
    10021 gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    10081 acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc
    10141 gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg
    10201 agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt
    10261 gccagatggt tcttatgaag tattgactgg gaaccaaatc ggggcattgt ttgctcaata
    10321 tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc
    10381 aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa
    10441 tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caattcgaag aagaccattc
    10501 acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg
    10561 cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa
    10621 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg gctattttgc
    10681 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt
    10741 gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac
    10801 agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac
    10861 accgccatcc gacgtattga aatatacatt ggaagaaaat ggctggattg ctatccgccc
    10921 ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga
    10981 cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa
    11041 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    11101 gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa
    11161 atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat
    11221 gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt
    11281 ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt
    11341 cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    11401 taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaaatttct tcgttgtcac
    11461 actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag
    11521 ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt
    11581 caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg
    11641 caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc
    11701 cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc
    11761 ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca
    11821 aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg
    11881 aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc
    11941 catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca
    12001 ataaatggag ggcaccaggt tgccgaattg gcttgatacc ggttaaacgt tcaatttctg
    12061 atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg
    12121 ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg
    12181 catcttgcaa tgcctgcttt aatgtttttt catctgtcac gaacgttctg cggaataggt
    12241 tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc
    12301 cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata
    12361 aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta
    12421 atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag
    12481 aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt
    12541 tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat
    12601 tcaaaggggt aagttatgat acgatttatc ggaatctcca tgattttgca gagatcgatt
    12661 tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcaggggg
    12721 aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata
    12781 tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat
    12841 tcgagatttt cggaagatgt gaaaattgtc aataagggtt taaataaaat aacatttgat
    12901 aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac
    12961 aggacagtct tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg
    13021 gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc
    13081 tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg
    13141 tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg
    13201 caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta
    13261 aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga
    13321 atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa
    13381 gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt
    13441 gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa
    13501 aaatatatgc cggaacaact ttccgatgaa gaaattcgac aacttgttca agaagcaatc
    13561 actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct
    13621 aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa
    13681 tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa
    13741 aaaagaacta aaaa
//
LOCUS       DADXTG010000065        13676 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.066, whole genome shotgun sequence.
ACCESSION   DADXTG010000065 DADXTG010000000
VERSION     DADXTG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.066"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..860)
                     /locus_tag="IWI57_001654"
     CDS             complement(<1..860)
                     /locus_tag="IWI57_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449800.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEE"
     gene            complement(860..1129)
                     /locus_tag="IWI57_001655"
     CDS             complement(860..1129)
                     /locus_tag="IWI57_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449801.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(1126..1506)
                     /locus_tag="IWI57_001656"
     CDS             complement(1126..1506)
                     /locus_tag="IWI57_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBC2449802.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(1973..3436)
                     /locus_tag="IWI57_001657"
     CDS             complement(1973..3436)
                     /locus_tag="IWI57_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBC2449803.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(3500..6517)
                     /locus_tag="IWI57_001658"
     CDS             complement(3500..6517)
                     /locus_tag="IWI57_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2449804.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(6668..7507)
                     /locus_tag="IWI57_001659"
     CDS             complement(6668..7507)
                     /locus_tag="IWI57_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2449805.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            7652..7897
                     /locus_tag="IWI57_001660"
     CDS             7652..7897
                     /locus_tag="IWI57_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449806.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            7960..8382
                     /locus_tag="IWI57_001661"
     CDS             7960..8382
                     /locus_tag="IWI57_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449807.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(8476..8793)
                     /locus_tag="IWI57_001662"
     CDS             complement(8476..8793)
                     /locus_tag="IWI57_001662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449808.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(8820..9026)
                     /locus_tag="IWI57_001663"
     CDS             complement(8820..9026)
                     /locus_tag="IWI57_001663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449809.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(9007..9189)
                     /locus_tag="IWI57_001664"
     CDS             complement(9007..9189)
                     /locus_tag="IWI57_001664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449810.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(9203..9553)
                     /locus_tag="IWI57_001665"
     CDS             complement(9203..9553)
                     /locus_tag="IWI57_001665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449811.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(9579..9881)
                     /locus_tag="IWI57_001666"
     CDS             complement(9579..9881)
                     /locus_tag="IWI57_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449812.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(9884..10117)
                     /locus_tag="IWI57_001667"
     CDS             complement(9884..10117)
                     /locus_tag="IWI57_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449813.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(11103..11425)
                     /locus_tag="IWI57_001668"
                     /pseudo
     CDS             complement(11103..11425)
                     /locus_tag="IWI57_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11548..12663)
                     /locus_tag="IWI57_001669"
     CDS             complement(11548..12663)
                     /locus_tag="IWI57_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBC2449814.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(12669..13067)
                     /locus_tag="IWI57_001670"
     CDS             complement(12669..13067)
                     /locus_tag="IWI57_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBC2449815.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3713 a         2783 c         2080 g         5100 t
ORIGIN      
        1 tgttcttcca attgaaaaga cacttttttt gtttcttgtg tctttttctc ggtttccaat
       61 gtaatgacat acgcaatttg ccaatgcttg ccttctttct tcatttccca ggataaaata
      121 cttttgactt tttccttttg aggactgatt tgtttcgcaa ctttttccgt tagaaacgat
      181 tgaagctttt tcctataccg ttcttctgcc atttcacccg aataataatt cgtaataaaa
      241 taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa gttcttccgc aaatgcaatg
      301 cgttcttgta actggtgctc ctttttcgtt agttgttcaa tctctctttg cgttgaaagt
      361 tgcataataa aaagaccaca agatacaacg acgataaatc caaacaaaag ccattgaaaa
      421 gacatttttt tcttctgaag agagccgttg ctttgtgtaa acgctgtttc tacagaaagt
      481 ggtgttttct tttccttttt gatttttgtt ggtcgacgtt cgttcttttg gtctttcttt
      541 acttgataaa acttcggtaa ttcggaattt aaggtttgaa taaaggtttg tgcttcttca
      601 cttaattgcc cattttctgt taaagattgt tcaatcaatg aaatcaaatg aaactcttct
      661 actggaaaat catacgagcc ttcaaatact ggaatttcat ggttttctgt gtagatttcc
      721 aaggtacacc agccaatttc cgctcgtgcc aatagctctt cattttctct tttcaattga
      781 ataaatgcct tatctaatgc aattttttta tctgttagta aagacatgtt atcaacttct
      841 gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac cacttctacc gcatacacca
      901 cttgaatagg gttatttaaa gaaaatttca cccgatattc ttgattgttc aaactgacga
      961 tatagctatc ctcatcttca gcataatagg tatattcgtt gcctttgact tgaaaatatt
     1021 tttggatcgc ttgtctttgt tgccattcgc caatctttgg aaaataggcg aaatgatagc
     1081 caaataaaac gcctatccct aataaaacaa ttcctgttac tataatcatc tggtttcctc
     1141 ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag caataaagaa ccagtaacac
     1201 aagagagcca atcgttggga caaaatagaa aaaaataaat gcccaatgaa aacctgcgtc
     1261 tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga actacgaaac aatacacaat
     1321 caatacgcct atcaaacaac caattattgt ttcagaagca ttcaacgctc ctgctagtcc
     1381 aatcgctaag cctaggccaa acccaatgag ggcattcatt aacgcaacga gccaatactc
     1441 ttttcttgtg gctttccctt tgtaattaaa gctattctgc caaaattttt tataatttgt
     1501 gatcatatct ttgtcctttc taatgtttat ttttgattct agtttcttct gttttactaa
     1561 caatgaaact tgccataaaa aaggtggcca atacaccacc agctagaaat ccacttacaa
     1621 ataacaaaat tttttcctcc tttctaaaaa atttcgccat aaaaaggcaa agtatgaaca
     1681 cgataaatac catgtacata ctttgccttt ttatgcaata tatgttaact ctccaagcaa
     1741 ttactgattt ttaaattctt tataaataaa ataaaaatcg tttgagtaaa gaaataagtc
     1801 aatgaagacc caaacgataa aaaatattcc taatactata tttaataaac tacgttctag
     1861 atagatacga gatttcaaat taaaaaacga aaatgagaga tacaaatata tcaaatattt
     1921 aaaaagtaac cacattttct ataccactcc ttatcagaaa cagccttatt ctttagaata
     1981 agactgtttc tgggttgatt aatttgtctt ttgttaaact accatctaag gcaagttcta
     2041 aatgtaaatg tggtcctgta ctatttcctg ttgaacccac ataaccaata atttgtcctt
     2101 gttcaactgt ttgtcctacg ttcacgcaat attcctgttg gtgcgcataa agagccacac
     2161 ttccgtcttc atgctcaatt gtgacataat ttcctgacaa agtatgatac gaagcttcta
     2221 tgaccgttcc tgccttgctc gcatagattg gttctccttg attggctgcc atgtctaatc
     2281 ctctatgaaa ttcttctcct cgccaaccga aggaactaga aattgaataa ttttttagag
     2341 gtaccatata gccttctttg cttagttgtg gaccagctac ttctttatca tatttgatta
     2401 agtcataggt ttcgattaac ccatttaatt ttttatcgta gctagtgtct gttgcgtatc
     2461 ttcctgttaa aaatttagtc gcttcctgat aggtttttgc cttacttttc caaacaccat
     2521 cataaaaaga ggtatttcca actaagcctt cttttaacag tttggcatag tcttttaagc
     2581 tttcttcata gctctcatat tgccgaaaag ttgcttgggt cgtataaagt gtcccattgc
     2641 ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt atgtgtgcct ttgattccaa
     2701 atagattgta gtttggtgcc tgagccaatt gactttgtcc gcttgcggat tctaaaatgg
     2761 cttgcgcgat cataacagaa gcgtataaat cattttcttg tccaatttta cgagcagatt
     2821 caccgatttt acggataaac gtttctacag acgcgtcttt ttcaaaatga atagtccctt
     2881 cgttatcttg tgtgctggaa gggactaatt cttgtatcgg tgcgcttggt gcttggtggt
     2941 agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg tacgtcattc ataggtacag
     3001 tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt tgttggcggt tccgcttctg
     3061 tttcggtact tggtttttga ggagtctgtg agctttcatc agaaggttgc tcatttggtt
     3121 cttctgacgg ttcctcctca ggttgttgat tgtcttctgg tttgtctggt tgattcggtg
     3181 tttctggttg ttcttcagaa cctgttggtg gagcgttcat ttctggatta tctggtgtat
     3241 ctattgtatc ttcagtcggt gtttctggtt gattttcttc tccagtggaa tttgttgtcg
     3301 tttcttcagt gatcgttgca gattgcgaat ttgtcagttg attgttgcct tgttcattag
     3361 ccaaaaccgc tggcgtatac ccaaatactt gacctactaa taacaaagca gataaacatt
     3421 tactcgtttt tttcacaaga ttgcctccat tcttcaatca atcatctaga gacagctgat
     3481 tgctgtctct agtcatactt taattgcttt gtttcatttt acgacgaacc agaacagccc
     3541 ccacaccaac aaaaagaaac agtcccaatg caattaacac atcgttcact gtttctcctg
     3601 tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg tttgacaggg gttgtcggcg
     3661 tttctttttt aatgcctttt gctgtcaatg tttgtttttc attcttcaaa tcaaagtgac
     3721 gtgcttcttc ctctttatgg tctttatcct tatagacaat atgagttgcg accaatttct
     3781 ccccgtcttt taacgtgcct gctttgtagt caaaggatac tttaaactct tcagacgctt
     3841 tagaaacttt acgtgcagtt tctaccgtac caacgactgt atcttttcca tttttatcaa
     3901 tcttatggaa ttgagtcacc cagtatttgg tttcccctac cgtagattct ggaaattctt
     3961 gcgatacatg atcgatcaac tcttggtctt tgtctggatc aaatgtttgg tcgccattca
     4021 ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt cactgtttga tctttattgg
     4081 ttaaatcaaa atgacgagcg aattcttctt cctcttcttt gtcgttatag acaatgtgcg
     4141 ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt gtagttgaac gtcacatcaa
     4201 atgtttcttt ttcatcggtc acttttcgtg tttcttttac cgtatctaat acagtgactt
     4261 ttccatcggc agtaatttta tggaattgcg tcacccaata tttctctgtc ccaaccgcag
     4321 atttagggaa tacttgggac actttatcca ctaattcttg gtcttttttc gggttgaaat
     4381 cttttccacc tgtcgttgtc acaaacaaag tagaaatttc tggttggtct tcttctggaa
     4441 ccacgatttc ttcgtttttc acagtaattg gttgtacttc tccgtctttg accgttacta
     4501 aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc agggaacaca atttccgtaa
     4561 atttatacgt accgtctggt aattttagtt tttctggttc ttcttttgtt tcatacacaa
     4621 aggacacatt tttattatgt gcagtcatac cttcaatcag gaatttcgct cctggtaatt
     4681 gtagatgatt gattgaatcg actttattga agtctacttc acccgttttt aacaagttgt
     4741 caatcacatc taattcaaac gtaagcacag gcgtcgtgtt atcgccatag gccacagaga
     4801 acgtggtttg ttcttcagaa ggttgacggt agttgtctgg cacatttttt tcaatcaatt
     4861 ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc cttccagtgt ccgtctttgt
     4921 cagtttttac tgaatccaag acttctcctt ttttcacaag gactgtttct ttatctggcg
     4981 cagtaatatc tgttttggcg ataatatcaa attcgatatt ttctgcagga acggtatcat
     5041 aaacaggttt gccttgattc caggattctt ccgcattata gacttctttg actttctttc
     5101 cttctggaat gaactgttgt aagtcatttt ttgcagttaa cgtcacttct ttgtttggtt
     5161 caatcgtaat cgtatgagtt tgtttgtcta gaatataccc agctggtgct ttgacttctt
     5221 tgacatccaa tttattttga acatattttc cgtctttttc atagaatgca gggatttctt
     5281 ttaaggcgat ttcccctttg gcatccgttg tatattgttt tgtttctccc gtatcatgac
     5341 gtttgatttc atacgtcaca tttggaactt ttttccctgt gacagcgtct agtttggtta
     5401 gtttgaagtc gccatttttt agaactttaa tagttagtcc agctctatta ggatctggca
     5461 cataaagctt tcctagattt tgagcaacag gatctacata ggcaaaactt gaacctaccc
     5521 ctctagaatt tggatccagt gaagaaacag aaattcgtcc gtcatttgag ttttgatttg
     5581 cttttataaa caaactattt cctgattttc ttacttgaac gttagctgtg ttttcaacaa
     5641 ccgacccatc ccattgagaa aacacacctt ttgtatcctt aacttctact tcttccccta
     5701 ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc aagtaaattg tttatttttc
     5761 tatctacagt ctgtttgaaa gcttcataat tagattgtcc aatagttcca taattaacaa
     5821 atgaaccacc taaaacttcc caaatatatg tttgtgctaa tacatagtta tccattgaac
     5881 cactagaata gtagccaaaa taaccaatta aagaaatctt ttttctaata gcacttgtcg
     5941 taattaaatt ttcgagtggt gtcacatcat attgaatacc cgcactcgtg attgcgcctt
     6001 cttgaatgca aaaaacatct tctccgtcaa tttgccagcg aaccatgtaa tcatgagtat
     6061 acattgttcc ttcaggagta attcgagtca tattcacatc tttacgacca ttttcagtca
     6121 atgttagatt ggtagcacgt gtctgtgcat tcttttccaa gattgcttgc cattcttcag
     6181 attcttgcgc caatcgttct tgttcagaaa aagaagtcgc tttttgaata tcttcaatgg
     6241 ttataaagta tttctcataa ccttcaggga cctcttgagt cattacattg ggcggagaaa
     6301 catcttctgt cgtgtcctgc gtagttgtct cttctgttgt ttcacttgtg gtttcagtac
     6361 tgatcgcttc ttcactactt tttgattctt cagtcttcat tacttcttca gtagaatttg
     6421 attcatttat cgtttctgca aatgccgtta cagatcctaa catgggattg agaaatgtaa
     6481 cgaaaattgc caaccaagtt gccattcttt taaacatatt tttttcgttc ctttcatttt
     6541 atttttcagt cttctaaagt aatttcttat ggacttcgac accgaaacat ttttatcctc
     6601 ctcaaaacaa aaaaagacta gaacttataa ttctagtctt catcaaccac ttacttttct
     6661 tttattttta gtagaaatct acagtgtgtt tttgtgaccc gtcactgtaa ataataacat
     6721 ccaccataaa acctttgttg taccactgcg aatttggatc gctaacctct gcattagccc
     6781 aatcataggc ttcatctaca gtgttaaata cctttccact attaccaatc tctccaacta
     6841 caattgttgg ctcttcaggt gtcgttgtgc caccatttcc gccttgatcg cctccaccag
     6901 gattgcttgg ttcttcaggc gttgttgttc ctccacctgt gcttgggtta gaaggagttg
     6961 tttctcctcc agtatttcca ccattacctg gttgtgtagg tgtttgagta gtgccaccat
     7021 tgttttctgg tgtacttggc gttgttgtac caccagttgt tcctgtttga tctccagctg
     7081 gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt tgaagtcgtt ccttgtccta
     7141 ctggttgatt cggatcaact ttgtctttct cttgaatcac tgtttgagcc acagtttcac
     7201 ctgcgctgtt ttgtacactt gctacattgc cattaaatac taatttattt cctgcataga
     7261 ttaaatccac atttgtaatc ttattcatgt ccgctaattt ttggacagta atatttgttg
     7321 cttgactgat tgcgcttagg gtatctcccc agacaattgt gtattcattc cctttaattt
     7381 ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt gttttctgct gcttcagcat
     7441 tcgttcccac agcaagacta actcctccga ctacgattcc tagtgccaat actttcaaca
     7501 ttttcattgt ttttcgcctc cgatttgaat aaatttattt acaaaaacat agtaccaaac
     7561 ttatgtctat cagtcgatat attttttgta gattttttta ttttttgata tactaacttt
     7621 ggtactaaaa taactaaaag gaatgtcaca catgcaactt aataatttaa atgaaataca
     7681 tgattgggct caaaataatt tacttaatcg taaacaagct gccaaaatca ctggacaaaa
     7741 ctatgatgct ttttctagct ctttacgtag aaataatatc aaaccatttc tagaaatagg
     7801 cgacgaacct ggtccttcca tcattcgctt atatctaaga gaagatattg aaaaatacgc
     7861 aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc gttttctgtc ttggatttac
     7921 aagtaaaaca ataataaaca cttttacaac ggaggttcta tgaataagaa atacattgtt
     7981 tcagttggtt caatcgtact gattgtactc gctattattg ctttttctgt atccaacaaa
     8041 acagaaacac agccacaaaa tacggttcaa tcgactacta caagtcaatc tagcgatcat
     8101 tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg gacaagattt aattgatctt
     8161 ggttcttctc aaattactac tgtcttattt gaaaagaaaa atattgcgtt tattactcct
     8221 aaagaaggaa ctgaccttga attttctatc aaagtaacaa ataccaaaga aaatttattc
     8281 tccattcctg acattcaata tacccctaac aagttaaaga ttggggatac gtttggtttg
     8341 gctaaagtaa atgaacaata ctttgcttac tcactgaaat aagtttctaa tagcaagagg
     8401 aaaaaccctc ttgttatttt tatcacataa tcattttata aaaacgcttc gaaaatatca
     8461 acatttttga agaaactatt tttctaattt ctcaatttct tccaagggat tttcgctaat
     8521 gacccataca ggcacagatt gctctcgagg ctccccttct tctaaccacc aacctgcata
     8581 atgaacttct tcctcctcag cattaccatg ataccaacca acaaaggatt catctacaac
     8641 gaatgaaatt tcttcttctg gatcaaataa ttcatttttc catgcttcaa ttgcttgttc
     8701 tttggtgtac ttttgtttgt taaatacacc acaaatttcg tcatctacat aaaaaatttc
     8761 aaaatcaaaa ttactcgttc cttttactgg catcttaact tttcctcctt tgactcactt
     8821 caatagatat accactcatg ttcattccat tgtttactag ttaattggag ttttatctta
     8881 gggctttgtg cactctttcc aaaaacttga gcgtcgtttt tttcttctaa ccaaaaaagg
     8941 taatttctta cattcggtac taaatgtttt tcctgcattt cttcatgaaa aacatataat
     9001 tctgcttcat ttttcaacca attcattatt cattccctca ttcacttctt cataccgcca
     9061 ttcagatagt gagatttctt tttgtttctc cacttgtcct ataaaaatcg ctgtattcaa
     9121 caagctgatc acacttaata ataaagcaat gaaactaatg gttacactta ctctttcaca
     9181 ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc ttgtaatagg tctaataact
     9241 ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat aaaagcactc gcacacatta
     9301 ccagaccttc aactgatata tcactgcgac aataaaattc tggagaaaca ctgatttctg
     9361 attctttctt acaattgaac gaaaaaacta tttttgaatc aaattccggt aattcttcca
     9421 caactatgtt ttgggattct accaagaaag ggagtgcctg ttctaatgct tcttctggtg
     9481 tttgagcaga tagaataatt gcttcttgtt cttttatttc attgttctca aatactttat
     9541 aacttttttt cattgttagt tcctctttca ttcctctctc aattagctaa gccgttactg
     9601 gtacgccatt ctctaaattt agtatcgcta atccttaatg ctcgtcgaat atcaatcatt
     9661 cgataaccta ttgcacgaag ttctttatat cttttagcag ttagcgtctc taacgtcatt
     9721 tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga ttatttgggc caattgttgt
     9781 acttgtaaca gctcttctga acttaattca gtgattgctc gattgcctat cattgcatac
     9841 attttctgtc gaatatactt cttttcttgt ttagacaaca tttctatcca ctcctatttt
     9901 cattatcatt tgttacctcc acttcagtaa atccacaaat aaatgaattc atattccaag
     9961 taccattccc ttcaactaca acttttctcc tgtcctcttt cggatattca agaatcagac
    10021 cgtttaccaa tatgtttttc actactacta atttgtttgt ataccgtgga attgctgttc
    10081 cgatatactt cactttattt cctacgctaa ttgtcatttc ctcactccta ttctttttta
    10141 tccaaaactt tttcctgtcc tattcctgcc aacgaatata cttcactgca tgcttaaaca
    10201 aaacaaaatg ttgccctgtt ttatttccgt cttttacttg ttccacatac agttcatatt
    10261 tggtcacacc ttttaaaacg ccagatactt tttttcatcc attgtcacaa ttagtttttt
    10321 catcaatcga ttgatactgc actgctcatt tttcttgttc atatagtatt cctttctttt
    10381 tcaaaactag cctcttttgt ttactagttt tcatcaatca tcaacgattt aacagatttt
    10441 ttctttcgca gggtttctag cgtcagcaac cccaaactct aaaaaagtgc ctttgctccc
    10501 ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt tttcatacta cctgatccac
    10561 gcaaataacc tttttttgtt ctgtgccaca cccatccata cgtatcgctt gagattcacc
    10621 atactttttt aggtggttta tccttcctaa gtccctgtta gacacgctga ttaacttaga
    10681 aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag aaataagtag gtttacgtgt
    10741 atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt ctactcccct cgttcaaggg
    10801 attccttcca ccagctagct tttcctttca tcatcaaatg ttcctgaaaa gcatttacgt
    10861 ttcgtgtccg tgtgcttact tattcttttt tcgatagcga ttatccgctc gctactcgct
    10921 attttatact ttgtttgtta gctcaaataa aaactaacaa acaaaaaagg ctagtttctt
    10981 tccgttttat ttttttaact gtcgataaat agttgttagt ttaattccaa gttctaaaac
    11041 caatacgccc aacaaaacca caggaaaaac aattggggca aacaagtaaa atcggatagt
    11101 tttcatgtct tccctctttc tttttggtgt cgctgtggtc gttcttgccg ttcaatactt
    11161 cgatacattt tttattcatt atttgctcgt tttgttttcc tctggactgc ttttcttgct
    11221 ttttgaatac tttatacgct tttcgtgcct gaattaatag gtcttgtcgg cgtttcattc
    11281 gtgagaaaac aatatttgaa accatcaaat cctggttcga acaaccacga ttcgcaataa
    11341 ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa cagttggaaa tcttgttttg
    11401 ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt tgaatctaca atgattcttc
    11461 gaacaatccg attcgataac ttccaacgtt gccgattaaa caatgttact tcacaacaaa
    11521 tatgacgtaa gtgattaggg gtcattttca tgtgtccaat tcctccaacc aattatgcat
    11581 cgaaattttg ggcaattgtg acagcccata aatctgcgtt tccaggtctt cataactaca
    11641 agaacgcttg gcttgaagta atgaatattc tgcttgatct gatagttttg ttgccattgg
    11701 atctagattc aattctctta cacaacattc ccaaaaattc aatagcgctc taaaataaaa
    11761 tccttttcga ttctgaattg cttttccttc atgacgagca attttttcat tttgaataca
    11821 ccgttgatac gttttgctta aatcttctgc aaatgtttcc cctaaaattc gatactcgct
    11881 tccatcagat aaaccatgtg tttgattata ctttcgttgc cgcttttcta taattcgttt
    11941 actgtcgtat atcatgttta gctgctcttt cgcttcacgc cattcagctg aactgcgaac
    12001 aatccatgtc aaaacatctt tcggaacaaa cgtactttcg ctgtaatgag caataaactc
    12061 tttttgccaa tcatcagcca tttcaacagg actagtcgta tcgatttttg tatctagatt
    12121 ttctgtatct agattgttag aagaattatt aaataattgt gcagaatttt cacatggatt
    12181 tttttcgtca aaatcatcga aaatcgctaa attatcaaca gattcctcct cttcttttcc
    12241 ttgtgcagaa tctacactgg ttgagtcaac aggacctaat gaatacacat cattttctgt
    12301 cgtatctaat tctcctagat acaaccgagc tttgtttctt tttcctgttt gttgatcaaa
    12361 tcccatatcc tctatttcta gtagctcata ttctttcaat tcttttttga ttgtattaac
    12421 agaacgtctg caacaattca atgcattggc taattcttgt tccgtaaatt caaaataaac
    12481 aaaaccttga ctatctactt gatttcgttg aatggctaaa tctaagcgtg accgaaaaac
    12541 catatacgcg atttttgctt tatcagacaa tgagcgatat ttttcacttg taataaatac
    12601 ctttggaaat tgatagtata attcattgta tttacgagtt gcggtaaatc tttttgttgc
    12661 catcgttatt acacctcatc ttgtccatat attaagaaag aattttggtg ttggtagaca
    12721 cgaacatctt ccattgtagc aatcaattct tttagataaa ttcgaccgaa ttcaattgtt
    12781 agtaagtcgt aatcttctaa ttcatttttg atttcttcaa tttcttcttc agacacattg
    12841 agtaaaatgg ctaattcttg attggtataa gaaatataga catgtttctt ttcatcaatc
    12901 cgtttttcaa ttaaggattc ttctaagtac tcattcagta agctataggc gtattttgct
    12961 tgtgcagtca tcgcataata ttttgacgat tttataaaga atagttttgg catatgaaaa
    13021 gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt ttaccatatt ttttcctcct
    13081 agaaaaacac gcaaaaaaag ctctgtataa atcgaattac acagagtttc ttcttgttga
    13141 tatttaattt ctaaaatacg tttcgggtaa aaactaaaca cttactaata tttagattgt
    13201 agaatcttcg ccttcattta ttcattttaa agagaaaaag atcaagtgaa atgtgcccaa
    13261 aagtttgatt ctattaattt ttaaacacca ttcataatat atttcctata atgtaaaaag
    13321 cgtcgatact tattaaagca tcgacgttat ttttatagta aatttatcaa aatttttgcc
    13381 aacatttttt ccgatttttt tccctatttc tatctttcct tatctttctt agttaatatt
    13441 atttgatgtt aatatatcac tactaatatt aaaaaacatt gatttatcat aggataaaga
    13501 gtgatagttt aatacttaaa gagacatatt aattttattt aaaaattgac attaatgact
    13561 cttaatcagc gggtcgcggg ttcgagcccc tcacggccca ttgggtgcca aacccacgga
    13621 acaggtaaat gttggtgcga aagcatttcg aaaacattta cgctgttctt ttttta
//
LOCUS       DADXTG010000066        13604 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.067, whole genome shotgun sequence.
ACCESSION   DADXTG010000066 DADXTG010000000
VERSION     DADXTG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.067"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            217..555
                     /locus_tag="IWI57_001671"
     CDS             217..555
                     /locus_tag="IWI57_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449816.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(760..1335)
                     /locus_tag="IWI57_001672"
     CDS             complement(760..1335)
                     /locus_tag="IWI57_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBC2449817.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1944..2525)
                     /locus_tag="IWI57_001673"
     CDS             complement(1944..2525)
                     /locus_tag="IWI57_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2449818.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2708..3334)
                     /locus_tag="IWI57_001674"
     CDS             complement(2708..3334)
                     /locus_tag="IWI57_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2449819.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3356..4687)
                     /locus_tag="IWI57_001675"
     CDS             complement(3356..4687)
                     /locus_tag="IWI57_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBC2449820.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4842..5243)
                     /locus_tag="IWI57_001676"
     CDS             complement(4842..5243)
                     /locus_tag="IWI57_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBC2449821.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5329..5535)
                     /locus_tag="IWI57_001677"
     CDS             complement(5329..5535)
                     /locus_tag="IWI57_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449822.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5630..7132)
                     /locus_tag="IWI57_001678"
     CDS             complement(5630..7132)
                     /locus_tag="IWI57_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBC2449823.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7150..7605)
                     /gene="argR"
                     /locus_tag="IWI57_001679"
     CDS             complement(7150..7605)
                     /gene="argR"
                     /locus_tag="IWI57_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBC2449824.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7703..8320)
                     /locus_tag="IWI57_001680"
     CDS             complement(7703..8320)
                     /locus_tag="IWI57_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBC2449825.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8541..9914
                     /locus_tag="IWI57_001681"
     CDS             8541..9914
                     /locus_tag="IWI57_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2449826.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            9989..11101
                     /locus_tag="IWI57_001682"
     CDS             9989..11101
                     /locus_tag="IWI57_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBC2449827.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11173..12810)
                     /locus_tag="IWI57_001683"
     CDS             complement(11173..12810)
                     /locus_tag="IWI57_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBC2449828.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(12978..13586)
                     /locus_tag="IWI57_001684"
     CDS             complement(12978..13586)
                     /locus_tag="IWI57_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2449829.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3669 a         2858 c         2442 g         4635 t
ORIGIN      
        1 ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat aaattgtagc
       61 cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct atggatccaa
      121 aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga taaaacgctt
      181 tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa acaaaaagct
      241 ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa
      301 gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga
      361 atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat ttttgtagaa
      421 gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc tcttatctat
      481 aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag attccaagaa
      541 ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc gcagcacgag
      601 taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata gatgcagtat
      661 atgcagctta tgagcatttc ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt
      721 ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct ccattactaa
      781 ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta cccaaggttc
      841 gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga ctgtgcggtt
      901 aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc tacgataaaa
      961 accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt tttcttcatt
     1021 ccactcataa aagccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa
     1081 ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa
     1141 tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa
     1201 cgtcacaaca agatttgacg gaaaaacttc cccagctgct aattctgctg cctgcttcgg
     1261 ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa
     1321 ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga
     1381 agttcgcacc aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac
     1441 aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa
     1501 caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct
     1561 tacgacctct gggtaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
     1621 cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt
     1681 tttcacaacc tctccactaa acggtgttca atcagctaac cttcggtatt agctcaaaaa
     1741 acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct
     1801 gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa
     1861 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
     1921 acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg
     1981 atcaattgaa aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata
     2041 cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt
     2101 ttttcttcag aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat
     2161 tctagtagca acagacggta ttcttcatcc actgtactca actcaataat caaattacct
     2221 acaggacagc catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt
     2281 tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc
     2341 tgccatacgt ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag
     2401 taataataaa attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt
     2461 tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt
     2521 ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat
     2581 tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat
     2641 aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat
     2701 tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa
     2761 agccaaagtt cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg
     2821 tcgctaccac aacattgaat ccactctcta tcaattcacg catatgggaa tcgctacaca
     2881 agtttccgtt cattccacca agaattactg tgtcgattcc attttttctc aattggagcg
     2941 ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat
     3001 ccaaataagg tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat
     3061 attgactttt tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa
     3121 attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt
     3181 tttcttttgc ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga
     3241 tatcttttgt caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag
     3301 tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt
     3361 ttaatacatc atttagagct tcactcattg tcggatgaga atagatttga tctctcaata
     3421 acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat
     3481 cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga
     3541 atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga
     3601 ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata
     3661 aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat
     3721 tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata taagtgaact
     3781 gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt
     3841 catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg
     3901 atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt
     3961 cttggccatc ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta
     4021 cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg
     4081 cgatatcttc atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag
     4141 atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca
     4201 ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac
     4261 ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc
     4321 gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa
     4381 gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat
     4441 ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga
     4501 ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg
     4561 ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta
     4621 acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt
     4681 ccatcatttt tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga
     4741 taattgtcta ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg
     4801 tcctttcacg tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct
     4861 acatcgatat ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca
     4921 attttttggg cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc
     4981 tgatagccgc catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct
     5041 gtcattcctg cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct
     5101 gctgcaaata attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc
     5161 atatgcatcg agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt
     5221 gctgtttcgt atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc
     5281 tataattata gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac
     5341 cagaatgccg tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag
     5401 cgtcatattt gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat
     5461 tttaagatac atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac
     5521 catattcgtt accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc
     5581 ataccacttt ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg
     5641 ttaatgattc aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact
     5701 tgttctttcc gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta
     5761 aacggatcaa aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt
     5821 cattttccgg gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta
     5881 atatcttgtt tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta
     5941 cttctacggg ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta
     6001 attctggcaa aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt
     6061 tttccttgaa tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac
     6121 ggttaagttc taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga
     6181 tgcctccttt ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga
     6241 ttcttgttcc tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc
     6301 gattgatcac tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa
     6361 tgatcccaag atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt
     6421 tattatcagg atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac
     6481 ctgttgaatc cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat
     6541 ttccggcatc aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc
     6601 cctttttaat ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta
     6661 cgcttccagt ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt
     6721 ctttttctgc tcctccttct gaggtaaaga attttattcc attataataa tagggaagat
     6781 ggctagcagt cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa
     6841 acatagcagg cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac
     6901 tttggatcaa cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa
     6961 tcgtccattt tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc
     7021 cccagccgat cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta
     7081 atgcgattcc tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt
     7141 cctcctcatt tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc
     7201 tgagcggcaa ataataatga atgtatctac accggccatc gttccgacaa cttcagggta
     7261 agccacttca tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat
     7321 aaccatgaac tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg
     7381 ttcttcattt aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc
     7441 attttgactt tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac
     7501 atcggcgcct gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc
     7561 tgtaatgatc tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca
     7621 atgttgatta tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta
     7681 ttttttgttt tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca
     7741 ttttttcagg tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt tctttagaag
     7801 ctctttcata gccttctttt gcgatgaatg cttctaattt ctcaaacgtt gcaggttctt
     7861 cggcataggg cccgacgtgt aagatttggg cacaaagacc ttcttccatc tttttgaata
     7921 ctacttgttc tttcaagtct tccggaagtt tgctagaacg ctgaatggct tcttgtgcta
     7981 tcgcttctgt aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt
     8041 tcatcactgg ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc agtggataga
     8101 cagtatacat ctgataatca ggaatcagcc aatttttttt aggcgacatg cgtattgcat
     8161 agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc gggtcgccga
     8221 tccctctgat gctaaagaat ggttttttag gaacctctag tatttgcggg ctttttttca
     8281 tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct ccgcgaaatt
     8341 ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg tgttgacact
     8401 atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa cacgtttcgg
     8461 cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt tttttacgaa
     8521 attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta cctgcagtaa
     8581 ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg aatgtaactt
     8641 tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg gtaacaacga
     8701 tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat cgcaatttta
     8761 gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata atgatcgatt
     8821 tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt gcagcgacag
     8881 gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc aatcgacgag
     8941 gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt ggccccactt
     9001 atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt cttgtgcccg
     9061 ttttgattct ttctttgatt atcggctggt ttgctattcc cgagatggca gtacaaaaaa
     9121 gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt ggaagtctaa
     9181 catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc ggcggtattg
     9241 gctggttcct tttttactat cgaagtaaaa aagtggagca tccactgatc cgactgtcta
     9301 ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat cagtttttat
     9361 tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga agtagtcctt
     9421 ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt tctccttttt
     9481 ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg ctgattcttt
     9541 ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg ggtgtattga
     9601 ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat atgatgacca
     9661 ccggaatgaa tgcactgaat gaagaactac aaggagacgg gaacgcaatg ttcaatacct
     9721 tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc aattacgtcc
     9781 agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa gcagcattag
     9841 gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat ctgttctttg
     9901 ctaaaaaagc gtagattcca tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa
     9961 atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg gtggcgggat
    10021 cattggtatg accctagcca gttacttaga tacatcaaaa ttcaatgttg ctttatttga
    10081 tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt ggttgtcgaa
    10141 acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt tccctaaact
    10201 tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac ttttgcttcg
    10261 acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag aagaagcgcc
    10321 agaaattgga aacatcgaat ggctttcacc tgaacagaca tctcaagcac ttccgttatt
    10381 gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa aagcatattt
    10441 agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg caaaagtcac
    10501 tttgcataag aagagcgatt cttggctcat caagggaccg tttggagaat acacagctga
    10561 tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat taggctatgc
    10621 tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt ataagatgag
    10681 tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt tcatggatgg
    10741 aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg ctcctactag
    10801 cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc cagatcagat
    10861 aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag atttttcccc
    10921 attttttggc gtactgccag atgatccaag cttactggct gctagcggtc taggctcttc
    10981 tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa atactggtca
    11041 ttggtctacg gaagattatc aaaaggacat ctccacctat attacgaaaa atccatcata
    11101 gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt acttcttgaa
    11161 aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt ttgcttcatc
    11221 tagcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat caagtcgttg
    11281 tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa
    11341 atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt gcacgattct
    11401 ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat atccagtatt
    11461 caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt gatagtcttc
    11521 cctaagagga tagacacgga aaggattagc aaatggttca atgactagct catctagctg
    11581 tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga
    11641 cgctgctaaa actgggtcat tgatcttgat aattggcgga agcgaatcat ctttcatgat
    11701 ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg tcgcataacg
    11761 tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga ctttttctcc
    11821 tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa tggccggatc
    11881 atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc
    11941 attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt gaccttccgt
    12001 atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata cagccatcgt
    12061 ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa taaagccatt
    12121 gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc caaagtaacg
    12181 taatcctagc acagccgctg tattatggag agcatcaaac aaaatgagtc catttggaaa
    12241 tgtttcatgt gtccattcgg gatcacaaaa gatataaaga tctccctcat caaaggaaac
    12301 agacttttga taagtctcta agatcttcga attcgctggc tgaaaattca gcaaataaga
    12361 atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag aaaattcttc
    12421 agataggcca acgattactt ttgtatggta aaactggcgc ttgcgacttt gatacacagc
    12481 ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa agatccgccg
    12541 agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc taacatcagc
    12601 tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg ttcctggact
    12661 cccttttgct agttgataag ctgtcttcct gtcaaaaact tcatgaacat tattaccgta
    12721 aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac ttgtgatttc
    12781 gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa acaaaaaaac
    12841 gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa
    12901 cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct cggtttcatc
    12961 attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt agcagcatcc
    13021 catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt ggcagctgaa
    13081 tagtcgccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg tccgttagta
    13141 gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga gcttgaagtt
    13201 ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg
    13261 tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt tgttttgttt
    13321 tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc aacataaatg
    13381 cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga gttgtctcct
    13441 gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata aacttcatca
    13501 gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa agttgtacct
    13561 aaaacaattt tcttaaatga attcatatta aactcctttt tttc
//
LOCUS       DADXTG010000067        13309 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.068, whole genome shotgun sequence.
ACCESSION   DADXTG010000067 DADXTG010000000
VERSION     DADXTG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.068"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..766
                     /locus_tag="IWI57_001685"
     CDS             8..766
                     /locus_tag="IWI57_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBC2449830.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            826..1254
                     /locus_tag="IWI57_001686"
     CDS             826..1254
                     /locus_tag="IWI57_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449831.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            1300..1372
                     /locus_tag="IWI57_001687"
     tRNA            1300..1372
                     /locus_tag="IWI57_001687"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1333..1335,aa:Gln,seq:ctg)
     gene            complement(1522..1893)
                     /locus_tag="IWI57_001688"
     CDS             complement(1522..1893)
                     /locus_tag="IWI57_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBC2449832.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            2108..2293
                     /locus_tag="IWI57_001689"
     CDS             2108..2293
                     /locus_tag="IWI57_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449833.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(2376..2564)
                     /locus_tag="IWI57_001690"
     CDS             complement(2376..2564)
                     /locus_tag="IWI57_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449834.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2588..2851)
                     /locus_tag="IWI57_001691"
     CDS             complement(2588..2851)
                     /locus_tag="IWI57_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449835.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            3009..3266
                     /locus_tag="IWI57_001692"
     CDS             3009..3266
                     /locus_tag="IWI57_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBC2449836.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(3337..3528)
                     /locus_tag="IWI57_001693"
     CDS             complement(3337..3528)
                     /locus_tag="IWI57_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449837.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(3669..3989)
                     /locus_tag="IWI57_001694"
     CDS             complement(3669..3989)
                     /locus_tag="IWI57_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449838.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(4012..4446)
                     /locus_tag="IWI57_001695"
     CDS             complement(4012..4446)
                     /locus_tag="IWI57_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBC2449839.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            4781..5419
                     /locus_tag="IWI57_001696"
     CDS             4781..5419
                     /locus_tag="IWI57_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBC2449840.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(5743..5826)
                     /locus_tag="IWI57_001697"
     tRNA            complement(5743..5826)
                     /locus_tag="IWI57_001697"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5790..5792),aa:Leu,seq:cag)
     gene            6354..6854
                     /locus_tag="IWI57_001698"
     CDS             6354..6854
                     /locus_tag="IWI57_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449841.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(7044..7388)
                     /locus_tag="IWI57_001699"
     CDS             complement(7044..7388)
                     /locus_tag="IWI57_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBC2449842.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(7542..8462)
                     /locus_tag="IWI57_001700"
     CDS             complement(7542..8462)
                     /locus_tag="IWI57_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2449843.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            8649..9140
                     /locus_tag="IWI57_001701"
     CDS             8649..9140
                     /locus_tag="IWI57_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBC2449844.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(9164..11539)
                     /locus_tag="IWI57_001702"
     CDS             complement(9164..11539)
                     /locus_tag="IWI57_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBC2449845.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            11865..12491
                     /locus_tag="IWI57_001703"
     CDS             11865..12491
                     /locus_tag="IWI57_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBC2449846.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            12514..13218
                     /locus_tag="IWI57_001704"
     CDS             12514..13218
                     /locus_tag="IWI57_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBC2449847.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
BASE COUNT         4053 a         2599 c         2242 g         4415 t
ORIGIN      
        1 atttcaaatg aagaaaaaaa cgttcaaaaa actattgttt ctagggggca cagctgctct
       61 tttattaagc ggtacaagtt attacctaca tatccattct ccagaacata caatgaacac
      121 tgcacaaaaa agttcaacac aaaaaacgaa aaagacaaaa gagaaagcag aagttttact
      181 agatgttcca ttagagagcc agtttgattc tccttcttta gaaaatggct gtgaagtcac
      241 ttcgctttct atgcttttat ctttttatgg ttatgaaaca actaaaaacc agttagctga
      301 gcaattggat tacgtccctg ttttcaatgc agatgggact catggcgatc ctaatgaagg
      361 atttgttggt gatatcagtg gcggagactg ggctatggtc gtctatgttc ccccagttgc
      421 ctcccttgct cagcaaatcg tccaaacgga ttatcacact tttccaaaaa ccgatgctca
      481 actcgatgat atcaaaaaag cactatctaa aggaaatcct gtctggactt ctgtgactat
      541 caattttgaa gtcccagatg aaactgattt tatgacttgg accacaaata atggagaagt
      601 aagagtcact cctcttgttc atgcttgtgt agtcactgga tatgacaaag aaaatattta
      661 tgtcaacgat ccctatggtg taaaaaatcg agcagtaccg atcaacgatt tttcggcgat
      721 tttcactgca atgggtggac agatgctaac tttggagaaa agctagtaat tcaaacaaaa
      781 atcccttata ctcaagatac caaacctttg aagggagcgt agataatgga aaaattcaca
      841 cataaaaaaa tggatccaaa tgaaattccg attatttttg tccgtgactg caagggaaat
      901 gttcaaggta aagtctctat taatgagtgg aacgagcgtc gcagacctgc taccctcaat
      961 gaactggaaa tcaaattgta tcgtcaatct cttgtttatt acgcagatca agagtacgag
     1021 aaagccaccg atttattgaa atttctaatt gctcgaacag agtatactcg ctttgaatat
     1081 attgaacgtt tagcaaacat ctatcatatt atgaatgaac ctgtcaaaga atatcagtta
     1141 ttggacaccg tcttgtcagt agcagaactg attgcccttc ctgctggatt agaaaagaaa
     1201 ctagtacgcc gccttttacg agtaaagcaa caactatccg atcaagaaaa ataacttgcg
     1261 tttttttagg aaagtgctat gattattttg ttgaataaat gggatgtagc caaactggta
     1321 aggcacttga ctctgaatca agcaattgta ggttcgaacc ctgccatccc aatttgatgt
     1381 gcttgctgaa aaaatgaacc ccctgtaaaa gaacaatctt tttgcagggg gattattgta
     1441 ttatttttac tacttgtatt tagtttacat aattaaatta tgaataataa aatcgggcgt
     1501 attcttaata caaaaactga tttacccaat aattgctaat gctttctccg gaactttttc
     1561 tctaggaact tcctcatacg tttctatgtg tgctccttta ttgctaattt tcaaataatg
     1621 attttctttt aacacagaaa gaccattaaa ttcaaccgtt cgttctttcc cattggcatc
     1681 agcagctttc tgcacatacc ttgctgtacc atattcattt acttcctctg gtttttgtgt
     1741 ctttacatag acctctcctt tttcaactaa tggatttagc tgatcaagta taccagggat
     1801 ttctccataa cttccttcag tataatattt cagtccaaat agcaaaatac cactaatcgc
     1861 cgccagtcca atcaaatatt taatcacttt catctttcta cctccttgtt atatattcag
     1921 tatatgggtc agttaaagaa attccatccg tcccaagttg gagatttttc tcctacttta
     1981 gaagcgagaa tcgagtgttt ttttagtaaa aatgatcgtt atttgatatt attgacgtgt
     2041 ggataaatca gtttttctcg ttttgtccat gtaaaaaatc atgttgatag caaaggatgt
     2101 gttcaaaatg aatcaagaag aattatttca aaaagtcaag gaaatgatta agaacggcaa
     2161 tttcgatggc gcaaaacgat ttatcgaaga acataaagaa caattaggac cttataaaga
     2221 gaaagcccaa aatcttttaa aagatgtaaa tatcgacagt gtcaaaaaca aatttaaaaa
     2281 tctattcaaa taagtaaaac tttcactttt gtaaaatcaa aggaggacaa tgctgctgca
     2341 tcgtcctcct ttggttcttt tctgaatact tcatactaaa ataaacgtgt cggatcgtaa
     2401 tgataagtca cattgccgtt cagtgaggct tttcccacca catatttacg attctccaac
     2461 ttcagccacg cttctcgaaa agccattaat tcttctcttt ttacatcaat cttttcgatt
     2521 tttccatcga tcaaatcttg aataagttgt tcatatggat tcattttttt cctccatctg
     2581 ctgtcaatca ataagttgta aaaataggtt catatgcttt tttggtatct agatcttctc
     2641 tgactttgac tgtcgtataa ataatttttt ctactgggat actataataa cttccttcac
     2701 cattattgaa tcggtgaacg tctttctttg ctaaaatacc atttatttta ttcgtcagta
     2761 cacgttgttc gacactgtta cttacaacat agtaaatcac ccgaatatta ttgatcaaat
     2821 gcaaatcgat ttctaacact acatgatcca tgaaaatccc actccttctt tcactttgat
     2881 tatagcaagt ttctcctcta atttcaaaat cgatacagat tttaccggat aaatccacaa
     2941 aactgcttgc gccatttcgc acaaagtgtt attttgtatc atggacttta tttatcggag
     3001 gcaatagtat gatcgcgatc ggtcaatttg ttttttatat tccttttttt attatgataa
     3061 gcattctttt ctattacata aaatggacaa aaaagaagtt ttcagtttta ctggcctctt
     3121 tacctgctgt atattttact tatcaaattt tttcttttag gcattgggaa acaacgtctg
     3181 tattaataac acacatcatc gaattgacgc tttcagtaat ttttcttatt atttggatct
     3241 attttttata taagaatcag aattgaatcc ctcgcatgtt taaaaagaaa acgattaaag
     3301 atgcagcaaa tttctgccga tctttaatcg ttttttctat tctgttatac caaaaaaatg
     3361 tttgaatcgg tgaaggatcg ttccttgttt taattctata aattcttgtg cgatcgggta
     3421 cttttcttcc aaattttctt tgttcttttc gatcaggctt ttcgcttctt catatttttt
     3481 atctgcacat agtgctttta cttcctgtaa tacttttcct gcttccattt gcctcaccct
     3541 cttgccattt tattgcttta agttttattt aagttatacg cattattcta gtttcatccc
     3601 aaataacctt ttttcatttt tactcctcga actaatcagt tttctaaagg ctgattggtt
     3661 cgtctttttt atttttcctc catttcattc tgctcttctg ttaatttatc taatgctttg
     3721 attgatgctg cggctcttgc ataatcatag ctgtataatt ccataaattt atcaggcaga
     3781 acaaaccacg ctggatgata accgttagct gtgacaggga atcctttatc ttgttggtta
     3841 tctccatccc atctcagagc aacacctttt tttcctttcc actttactaa agcgatcgag
     3901 tatcccttct ttgtttgtgt gccgtcttca ataattgcta aaatttctat tttttctttt
     3961 ggcgcacgga cttcttgagg acgaaccatt ttctattcct cccttatctc attacttcac
     4021 gaccaacaca ctgcaagggg catgtgatac gacataagaa gcggtcgatc caactaatgc
     4081 ttgttgtata gcaccttttc ctgtcgctcc cataacgatc aaatcaatat tttcttgtcc
     4141 agggatgaca ttggcaatca aatttcttgg atctccagtt tcaactattg cgtggatctc
     4201 agcaatgccg tactctttgg catcattgat ttttttcagc atggctactt ctgttctttg
     4261 tttttcatct gcatagattt ttgaaaaggc ataggcacta tgggtcagct caactttgtt
     4321 gatgattgac acgataaata agtgggcttc attgtccttt gctaagtcaa gggcttcagc
     4381 gaacgctttg atcgattgtc ttgatccgtc aactgctacc atgatgttct ggtatctttt
     4441 actcattgga accgcctcct ttttctttta ttgtaacatc tcaagcaaaa atacaaaaaa
     4501 tatacgcaat atgtaaataa aatgatgaac aaaagcaatt tctttcaagt tatttataca
     4561 tttcttttct catcagaaca cctgttctaa tcccattatt tctgcattat gtgaaattcc
     4621 tcatcatttt tcacatcgag cgaacatcat tctgttacaa aaaacgtgga tttgtcataa
     4681 attgtcatgc ttcttttacc tatgaaatat tttcacgtgt taaagtattc ctcgttgaac
     4741 acaacaaata aaacttttta acgaataaag gagatttttc atgacatcac ttaaaacttt
     4801 actttttgga acaactttgg ctgccggcgc tgcattcttc atgggaacga ctgctcatgc
     4861 ggacgaagct tataccgttc aatcaggtga tactctatca acgatttctc aaaaatacgt
     4921 tggtgataac tccttgatca atgccattgc agaatcaaat tcaatttctg atattaacct
     4981 gatctactca ggacaacaat taacaatccc aacagaaggc tctgcacaag cagctgctga
     5041 acctcaagca gccgttcaag aagcaccagt ccaagctgaa cctgttcaag ctgaacaacc
     5101 tgttgtacaa gaaacggttc aaacggaaac acaagcagcg cctgttgctg aaacacaacc
     5161 tgctcctgct gtaacagaaa ctgctgctac tccagcaagt acaagctctg caaaagaatg
     5221 gatcgcacaa aaagaatcaa gcggttctta cacagcaacg aacggacgtt acatcggccg
     5281 ttaccaatta gattcttctt acttaaatgg tgactactct gctgcaaacc aagaaagagt
     5341 agcagaacaa tatgttactt cacgttatgg ttcttgggaa gcggcgaaag cattctggga
     5401 agcaaacggt tggtactaag attcattatt tgaacgttta aacacattca aaatatagta
     5461 agaggtcatg cgatttattc tttcgcatga cctctttttg agtttaagtt tagtctttat
     5521 tccttaacta tcgtttgcca gtatttcttg tatcagaatc tataaatcta ctatttgaat
     5581 caatctatta ggttttaaac tgtcccacct tcacacaaat tttttttgat ttatttgaat
     5641 ctggttccgt tgtttaaaat ttttcctata ttcttttaga taatatgtta ttttctttct
     5701 catcgctcca ggtgtacctt ctctttataa aaaaacaaat tatgcggcta agaagactcg
     5761 aacttccacg aggtcaccct cactagcgcc tgaagctagc gcgtctgcca attccgccat
     5821 agccgcatca catcttttag tttaggatta catgtgtttt ttgtcaagaa catatttttg
     5881 ttctttttca tttctgtacg ccctccctta tgccatctac acaaataatt catccgagca
     5941 actccatcat ctattgaggg ttggtgaaaa aatctgtttt atccatatga tcgatttcaa
     6001 tgttaaacga acgactctgg gattagtaac tattttattt ttttcttcat ttcttttttc
     6061 ctctttttct cgattcagct atcttctttc atttggatat tgattatttc ttctgttgat
     6121 tactatcctt ctggaattct agacgctgaa gtaattcacg ttttctttct tctagagatc
     6181 ataagtaacg aatgaacgaa cttcaaataa tttttttatt gtaattcata tttgtattgt
     6241 tcgatgattc ttaatacgat atgacacaat aaataaaaat aggcatggct atttacaaca
     6301 atagtgctat agtagaaaac aaatgaataa ggttacttaa agaggtgaat acgatgaaac
     6361 tgatcaaact atgggaagca gatttaaaga aagcttatca gctacaaact agctttggac
     6421 caaatgagag tggatttatc aacaatgctt atggctatac atttgctgaa tttttagatt
     6481 atgtaaaaaa atgcgaaaaa acgtctttag gaatcgatct tcctgagagt tacgttcccg
     6541 caaccgtctt tattttagaa agtgatgaac atgagtatgt aggaattttt aatttgcgcc
     6601 ataaactgaa cgaaacatta agaaacggcg ctggccatat tggttacggc atctccccta
     6661 attatcgtaa aaaaggatat gccacacaag gattagcatt agtactacaa gaggcaaaaa
     6721 aaatcggcat tgatgaggca cttttgtctg taaataaaaa aaatcttggc agtcttaaag
     6781 cacaacaaaa aaatggggct gcgatttatc gtgaagatga gaaagaatac tatacgaaaa
     6841 tcaaactgat gtaaaaaaga aggattccaa aaggaaaggg atttgaatgt cctttatcaa
     6901 tagaacacat ttatttaatt aacttataat atagcagaca aaattttata aacaaagaaa
     6961 ccttttatct cttttcttct agaaaggata aaaggctttt ttgtttttcc tatttgcttc
     7021 tcatagctag tcagccaaga tttctatcgt atccccaacg caatccgtgc ataacggctc
     7081 atcttatctg tcgtccatgc tggataccag actagtttga cttctgcttt cgttacttct
     7141 ggcacttctt ttaatgcgtc atggatagat tctgttaaaa tatctgctaa tggacagccc
     7201 atagttgtta gagtcatctt aatgactgtt tctccatttt caggattgaa ttctatatca
     7261 taaatcaagc ctaaattgac gatgtcgatt cctaattcag gatcaatcac catttccaag
     7321 gcagcaagaa tacgttcttt gattagttct atttcttgtc cagaccgttc ttggtttgct
     7381 tcgctcatcg tacgacactc cttaacacga tttttattta tcatactctt tttttagctg
     7441 cttgtaaatg agaatttaga agtaagattc tcgtttaaat ttataaaaat gttcttatta
     7501 gatgtaatag attaaattta ttctaaaata aacctgattc ctcatttttt ttgtgaacga
     7561 ttttttattt ctggcaatac gccagttgtt cgatacattt tttgttcgtt ttcatttgca
     7621 aaccggccaa atactgaatg agttggtaaa atattgataa atgcatcgtc gtcgatttca
     7681 tgaacgatct gatcaagatc atacatttcg tagcgggtga tgaccatcat gatcatgttc
     7741 ccttgttttt ttgaatagcc accgacagac ggcagtaatg tcattccacg taccatccgt
     7801 tttgctagtt cttctgttac ctcttctggc ttgacggtaa cgatcattgc tgttactttt
     7861 tgatgactcg tgtggatggc atcaattact tgagtcattg cataaatgga aataatcgta
     7921 tacaaggcac tttcccagtt aaagaagaag cctgcgatga ctacaatgat accatttaga
     7981 atcagcatgt agtttccgac tgtttttcca gttgtttgtg aaagtacaag agagatgata
     8041 tccatcccac cagttgtaaa gcccattttt aaagataatc ctacgccaag acctaataaa
     8101 acaccgccta ctagggcatt catcaaaata ttatcagtga tctgtccgac tggaagcagc
     8161 atcgtcatca ccgatacaca tacaacattc acaaaactaa ggatcgttgc tcgttttcct
     8221 actttgagaa accctaatac aaacacaggg atattcaaaa gaaaaataaa tgttcctgtg
     8281 tctatcttga tatgaaacca ttcatataaa agcgaggaaa tgatttgagc gatcccattc
     8341 atccctgcag aaaagacatt ggctggaatc aaaaaataat ttaaagcgac cgcagcggtc
     8401 aatccggtta tcaaaataac cactattttc tttaatcttt catttttatg atacgcagtc
     8461 ataattttac tcacaagcac ctctccccta ctttggtatt ccaaatgtaa gaataacagt
     8521 ttatctatta aaataaaata cttcgaactc aaatgagaag aattttagca aacaggtaag
     8581 aatctgccct aatcctatga taagatagat gtatggtaaa tatttatatg tatatcagga
     8641 gggttcctat ggcaaaagat gctagcttcg acattgtatc agaagtcaat acagaagaag
     8701 taaaaaatgc gattcagcaa acaacaaaag aattgtccaa tcgctttgac ttcaaggatt
     8761 ctacagtaga aatcaaatta gagaacagca atctagttgt attgagcgat gatgatttca
     8821 aaatcgaaca gatcaaagat gtattatttg gtaagctgat caaaagaaat gtgccaatca
     8881 aaaatatcca tttttccgaa tcagaacacg ctctaggtgg caaagctcga caaactgccg
     8941 atttggtcaa cggaatcgat cgtgaaaacg cgaagaaaat cactacagct atcaaaaatg
     9001 aaaaactgaa agtaaaagca caaatccaag aagatcagat tagagtgaca ggtaagaacc
     9061 gtgatgatct acaagctgtg attgctttat tacgtaaact tgacttacca atcgagttgc
     9121 aattcactaa ctatcgttaa tcaaaaaacg aggctgacgt ctgtcattcc tcgttttttt
     9181 gatttactgc ttcttttgat aaccaaaaac gctgtattcc ttcttccaat tccccaacac
     9241 ttcgtacttc tcttgctgtt tgccaatgtt gtgggaataa acgtcgataa gaaaaactag
     9301 aaaatcgttg atctgccaat acaacgattc cttgatccgc catgccacga atcactcgac
     9361 caccagcttg caagactttg ttcattccag gaagctgata agcgtaagca aatccaaggt
     9421 gttccttctc atcataatat gatttgatca gttcctgctc atgattcatc tgcggcaaac
     9481 ctacacctac gatcatactg ccgatcaatc ggtcccctct caaatcgatt ccttctgaaa
     9541 aaatcccgcc aagtacgcaa aatccaatca atgtttcagc gggatttgtt tgaaattttg
     9601 ttaaaaatcg ttcgcgttct tcctcattca tgtccgtttc ttggattagc acattgctgt
     9661 cgggatattg ctgtctgaat gcttccaccg caagatctaa atattgataa gaaggaaaga
     9721 aaacgaaata attcccgacc tttccatcag caaaagcatg aatcgtctct actaattttg
     9781 ccagactgtg ttcccttttt cgataagtcg tctctaggta atttgcgatg aaaacttgct
     9841 ggtttccttc tgggaaagga ctcggcagtt tgtaagccag acttttttgt ccccccagtg
     9901 tttcctgata ataagagagt ggtgaaaaac tagcagaaaa aagaatactg ctttttcctt
     9961 tatccaacga ctgctcaaga aacaagcttg gatcaataca aaactctttt actatcagat
    10021 cgcgatacgt tttttcgact gttgtttcgt agtgatcatc ataaaattca ctgaccttca
    10081 aaaaatgaag caaatcaaaa taataaggaa gcaattgttc ttgtgatggg tgctcaggaa
    10141 attctgccag ccattcttta attttttcgc ttaattgata acctgccttt actaaagatt
    10201 cagccggtgc tttttgatga tgataatccc aatgatcttc ttttgctatt tctcggatcg
    10261 aatcaaattc ttttaatagt ttgttgaaac ggcgatgaag ttttttgaat tcctttggta
    10321 tagcttcctt acttcgtttc gttttttcat aagatagttc tgccgaatac atctctcggg
    10381 aacgattcac caaattatgt gcttcgtcaa tcaaaaacag atagtcctcg tttttttcat
    10441 cttcaaaaaa acgtcttaaa taaacagttg gatcaaataa ataattgtaa tccccaatga
    10501 ttacgtcaca ccagatactg acatctaatg atagttcgaa aggacaaagt gtatgctttc
    10561 ttgcataagt ttcgatgact tcacgagtga tctgattctc atgattcaaa agatcccata
    10621 aaccttcatt aatccgattg taataaccat tggcatatgg gcactgatct ggattacagg
    10681 tcgtctcatc taaaaagcag atcttgtctt tcgccgtcaa agtgacactt tttacttcag
    10741 ctcctgtatc ttttaatgct gtcatagcat cttcagctac ttgtctagtt attgttttag
    10801 ccgttagata aaagatcttt tctccctctt cttcccctac agctttcaaa gcggggaata
    10861 acgtagagat tgtttttcct gttccggtag gtgcttctac aaacaatttc tgtttcgtac
    10921 ggatcgtttt ataagcagct accgctagtt cacgctgacc tttcctatac tcatcatggg
    10981 ggaatttcaa agccatcaaa gaggcattgc gtacagtgcg ccagttttct tgaaaaacaa
    11041 gccattcttt ataatcttca gtcagctttt taaaaaaagt ttctaactgc tctcttgtct
    11101 gatgttcaac ctttctcgtg atcacttctt ctgccgtctg gaagtaagtc aattgcagat
    11161 ttatttcttg taaatcatgt tcttgactat agatataggc atacaccctt gcttgatgaa
    11221 agaaaagttc cacttgttct ggttctaaat cttcaaatcg tggttcggag gttttgattt
    11281 catctatata ataaataccg tctcttttga aaataccgtc tgcacggcct tcaacgatca
    11341 ggtaatattc atctaattga actgttgttt ttaaaaagac ttctttttca tagttttctc
    11401 ccgcatcttt ttggagcttg cggtggattc tcgctccttc taatgcagta tggttacttt
    11461 ttttacgatt gtcgatactt ccacgtcgca aaataaattc tactaagtgc cgcacagata
    11521 tttttgactg tttgatcatt tccaccacct ctttctctct aagattacct gaaaaaacct
    11581 agtttagcaa aaagagctct ctttccaaac ataaaaaaac accctgctga ttaggcaggg
    11641 atataggata tagaatttgg atttttggat gaatctaatg taacaaggac atattaaaat
    11701 gctatgaaaa cttactttcc ttctctttag aatatccaaa tacttttcat atagtctgat
    11761 aattcagata agtaaagaga aataagtacc tgtttttgtt tcctagcaat gaatgcgtta
    11821 tactaatgga tgaaagaagt tttggctatc aaggaggaat gcacatgcat atcgaaagat
    11881 taaaaacagg aatgattgaa gaaaactgtt atttggtcta caacgaagaa gcacttttga
    11941 tcattgaccc tggagaagat gcggaacgaa tcaaacaaga aatcaaaaaa acaaaccaac
    12001 agccggtagc gattttgctt acccatactc actacgatca tatcggtgca gtggaacctt
    12061 tacgcgcgta ttatcaaatc ccagtttatg tcagcccact agagcaaaaa tggctaggtg
    12121 atccgatttt gaatctttct ggactcggta gacatgatga tattgctgat gttattgtct
    12181 cgcctgcaga atatgaattt gaaatgaaaa cttatacttt aggaaacatg tcttttcgcg
    12241 tcgtccctac cccagggcat tctataggaa gcgtcagctt tatttttgat gattttgtcg
    12301 tttctggcga cgccctgttt agaggaagta tcggtagaac cgatctgtat acagggaatt
    12361 tagaacaatt acttcatagt attcggaccc agctgtttgt attacctgat gaatttgcag
    12421 tctatccagg acatggcgat gcaacgacga ttgaacagga aaaaaggaca aatccttttt
    12481 tcaatcgtta aaacgactta ggaggagaat aaaatgacag aaactacact ttatatcgta
    12541 cgacacggaa aaacgatgtt caatacaatt gaacgagtac aaggctggtg tgatacaccc
    12601 ctcacaaagc aaggacaaga aggcattcat tatttaggtc gcgggttaaa aaatgtcgac
    12661 ttttcatttg cttattctag tgacagtggt cgtgcaatcg aaactgcccg tattattcta
    12721 ggagaacatt ctaagggaaa agaaattcct tattttattg aaaaaaggat tagagaatgg
    12781 tgtttcgggt ctcttgaagg tggatatgac atggaaatgt ggggtgtgat ccctcgtgta
    12841 ttgaatttta aaacatatga tgaaatgttt accacagaag tttcttttga acaaattgct
    12901 aatgctgtat acaacttaga taccgctggg tgggcggaaa cttatgaaac attgcgagac
    12961 cgtgtatgga caggatttga agatatcgcc catcactgcg aaaaaaatgg tggtggaaat
    13021 gttttagtcg tatctcatgg tttgacgatc gcctttttgc ttaatctgat cgatcctaaa
    13081 cagcctgttc gtgctgggct tttaaatggc agcgtaacaa aggtcactta tgcaaacggt
    13141 cgattttcta tccacggcat caatgatacg tcttatatcg ataacgggaa aacacatagt
    13201 cgcgactcgt ttctataatg gggatatttt aatttttacc agagaatcat aaaaggcaaa
    13261 gtctgaacga gggctttgtc ttcttttttc tcttcgtgta cttatctgc
//
LOCUS       DADXTG010000068        13199 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.069, whole genome shotgun sequence.
ACCESSION   DADXTG010000068 DADXTG010000000
VERSION     DADXTG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.069"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(240..3914)
                     /gene="rpoC"
                     /locus_tag="IWI57_001705"
     CDS             complement(240..3914)
                     /gene="rpoC"
                     /locus_tag="IWI57_001705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBC2449848.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3972..7589)
                     /gene="rpoB"
                     /locus_tag="IWI57_001706"
     CDS             complement(3972..7589)
                     /gene="rpoB"
                     /locus_tag="IWI57_001706"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBC2449849.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8317..9285
                     /locus_tag="IWI57_001707"
     CDS             8317..9285
                     /locus_tag="IWI57_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2449850.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9303..10208
                     /locus_tag="IWI57_001708"
     CDS             9303..10208
                     /locus_tag="IWI57_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2449851.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10205..11011
                     /locus_tag="IWI57_001709"
     CDS             10205..11011
                     /locus_tag="IWI57_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2449852.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11147..12136
                     /locus_tag="IWI57_001710"
     CDS             11147..12136
                     /locus_tag="IWI57_001710"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBC2449853.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12453..12977)
                     /locus_tag="IWI57_001711"
     CDS             complement(12453..12977)
                     /locus_tag="IWI57_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBC2449854.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
BASE COUNT         3664 a         2888 c         2599 g         4048 t
ORIGIN      
        1 aaaatattaa aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat
       61 ctatctttac ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg
      121 gatacgaacc gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt
      181 tagacaaacc aaaaaagagg ttgtgacaat aatttgtcac aacctctttt ttaaaatatc
      241 tattttgttt gattgttcat tgcgtcttct gcagccattt gcgcttcaat atctgaaata
      301 ctgtagacat tttcgcttgc tacaccgact tcttttggtt ccatgtcacg ataacgttgc
      361 ataccagtac cagctgggat gatcttacct ataataacat tttctttcaa accaagaaga
      421 ggatctttct taccgcggat cgcagcatcc gtcaagacac gagttgtttc ttggaatgat
      481 gcagcagata agaaactatt tgtttctaat gatgcttttg tgatacctag taagacaggt
      541 cgagaagttg caggaacgcc tcctgagact agcgtattgt agttttgatc tttaaagtct
      601 gcgatgtcta ataatgtacc tggtaagata tctgtatcac ctggatccat tacacgaact
      661 ttacgcagca tttgacgaac cattacttcg atatgcttgt cgccgatttc taccccttgc
      721 atacggtaaa cacgttgtac ttcacgaagc aagtagtttt caacagataa tacatcgcga
      781 acttgcaaca attgttttgg atcgattgat ccttctgtca atggtgcacc acggtggatc
      841 atatcgcctt ctgctacttt catacgtgct gtataaggaa cagtatacgt acgtgtatct
      901 gtttttcctt tgattgttac ttctttggta cgtgttgcag gatcttctga gatatcgatg
      961 acatcccctg ttacttctgt gatcactgct tgccctttcg gattacgtgc ttcaaagatt
     1021 tcttggacac gaggaagacc ttgagtgata tcgtctccgg caaccccacc tgtatggaat
     1081 gtacgcatcg tcaactgagt accaggttca ccgattgatt gagcggcgat cgtaccaact
     1141 gcttctccga cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca atgtttgcag
     1201 acaccatgtt ttgtattaca tgtgaagaca gaacggattg tcactgtttc gacacctgca
     1261 tcaacgattt gttttgcgat gtcttcggaa atcaattggt ttcctgcaat gatcaattca
     1321 tttgtttcag gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg atcttctaat
     1381 gattcgatga tttcatttcc ctcgcggatg gcttggattt caagacctcg gtcagtaccg
     1441 caatcttctt cacggatgat tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa
     1501 cctgaatcgg cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc gtgagttgag
     1561 ataaacattt ccaagacgga tagtccttca cggaagttcg aaatgattgg caattccatg
     1621 attcgaccat taggagcggc catcaaacca cgcattccgg caagctgtgt aaagttggaa
     1681 atgttaccac gagctccaga atcagacatc atgaagattg gatttttcgc ttccaaacct
     1741 tccatcagtt tttgttggat gctgtctttt gcagcattcc aaacagcaat gacacgttca
     1801 taacgttcgt catctgtgat cagaccacga cggaattgtt ttgtaatagt ttcaacttgt
     1861 ttatgggcat tttcaatgat ttcttgtttt tcattcagta cactgatgtc agcgatccct
     1921 actgtgatac cggcatgtgt cgaatgtttg taccctaagt ctttcatacg gtcaagcatc
     1981 ttagaagttt ctgttacttt gaatcgtttg aagacttctg cgatgatgtt tcctaagttt
     2041 ttcttcttga acggtaatac taattcttgc tctttgatat gtgcaggaat atcagtacca
     2101 gcttcaacaa agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt tggttcattc
     2161 agataaggga attcaggagg catgatctcg ttgaagatga tcttaccaac tgttgtgatc
     2221 atcgtacgtt ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt agggttcaca
     2281 ccgatacgtg aatgtaaatg aacatatccg ttgcgccatg cgataacggc ttcgttttga
     2341 tctctgaaga ccattccttc gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta
     2401 cctagaacca tatcttggga aggagtaaca actggtttac catctttagg gttcaagatg
     2461 ttttgagcag ctaacatcaa catacgtgct tctgcttgtg cttcttcatt caatggaacg
     2521 tggacagcca tttggtctcc atcgaaatcg gcattataag cttcacatac aagtggatgc
     2581 aaacggattg cgcgtccttg tactaaaact ggttcgaatg cttggatacc aagtcggtga
     2641 agcgtaggtg cccggttcag caatactggg tgttccttga ttacgtcttc taaaacatcc
     2701 catacttcgt cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt
     2761 tcgcgttgaa caagctcacg catcacgaat ggtttgaata attcaatggc catttctttt
     2821 ggaagtccac attggtacat cttcaagaaa ggtccaacaa cgataacgga acgacctgag
     2881 tagtcgacac gtttacctag caagttttga cggaaacgac cttgtttccc tttcaacata
     2941 tgagaaagag atttcaatgg gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg
     3001 ttatcgatca atgcatctac tgcttcttgc agcatacgtt tttcattttg aacgatgatt
     3061 ccaggtgcat tcaagtctaa caaacgtttc aaacggttat tacggttgat tacacgacgg
     3121 taaagatcat tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt
     3181 aaatctggtg gaataacagg gataacgtcc ataaccatcc agcttggttg atttcctgaa
     3241 gcgcggaatg cttctaaaat atccaaacgg cggattgcac gtgtacgttt ttgtccttgt
     3301 gcagatttca attcttcttt caattcggct gcttctgcat ctagatcaac attatcaagt
     3361 aattgtttaa tcgcttctgc acccatagca gcttggaatt cttgtccata ttgttcacgt
     3421 ttttcgcgat attcgcgttc tgtcaacaat tgttttttct ctaaattcgt atttcctggt
     3481 tcgatgacta catatgaagc aaaataaatg atttcttcca aagcgcgtgg gctcatgtct
     3541 aatacaagac ccatacgtga aggaatccct ttaaagtacc agatatgtga aacaggagct
     3601 gctaattcaa tatgtcccat gcgttcacgg cgaactttcg aacgagtaac ttccactccg
     3661 catcgatcac aaacgattcc tttataacgg atacgtttgt attttccgca ggcacattcc
     3721 cagtcttttg taggaccaaa aatccgctca cagaacaacc cttcgcgttc aggttttaag
     3781 gtacgatagt taatcgtctc aggttttttt acttcaccgt aagaccagct tctgattttt
     3841 tcgggagaag ccaaacctat ttgcatactt tcgaatttat ttacatcgat caaaaggggt
     3901 tccctccatt tcatttaatg gcaaaccgca agacggctga tcttcgaccg ccttgcagct
     3961 catagaccgg attagtctaa attatcttct gcggtttcaa agttttggat gacatcgtct
     4021 ttttcatctt ccacttgttc agcagctttc ttttctaatt ctttggcagt ttgctgttcg
     4081 gcaaattttg tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga
     4141 agttcgatct ctgtttcttc gatatccaag acacgcatgt ccagacctag tgattgcaat
     4201 tctttcacca atacgcggaa tgattccggt acacctggtt ttggaattgg ttcacccttg
     4261 acgattgctt cgtatgtttt cacacgtccg actacgtcat ctgatttgta tgtcaagatt
     4321 tcttgtaaag tataagccgc accgtaagct tccagtgccc atacttccat ttcaccaaaa
     4381 cgttgtccac caaattgagc tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt
     4441 ccgattgaac gtgcatgcaa tttgtcatcg accatgtgag caagtttgat catgtacatc
     4501 acaccgacag acacacgtcc atcaaatggt tcacctgtac gtccgtcgta cagaacagtt
     4561 ttcgcatcgc tggccatacc ggcttcacga actgtttccc atacgtcttc atcacttgca
     4621 ccatcaaata ctggcgttgc tacgtggata cctaactgac gtgcagccat acccaagtgt
     4681 aattccaata cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt cagcatgata
     4741 tcgattggtg ttccatcagg taggaatggc atatcttctt ccggcataat acgggataca
     4801 acccctttat ttccgtgacg accggccatt ttatcccctt catggatttt acgtttttga
     4861 acgatataaa cacgaactaa catgtttacg cctggtgaca attcatctcc agcttcacga
     4921 gtaaagattt tcacatcgtg aacgataccg ccgccgccat gtggtacacg taatgatgta
     4981 tcgcggactt cacgagcttt ttcgccaaag atcgcatgca atagacgttc ttctgcagat
     5041 aattctgtta ccccttttgg cgtgactttc cctacaagca aatcgccatc ttggacttct
     5101 gcaccaatac ggatgatacc catttcgtct aaatctttta atgcgtcttc cccgacatta
     5161 gggatttcgc gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc tgattcatat
     5221 tcttcaatat ggatagaagt gtatacgtca tcttttacca aacgacggct catgatgatc
     5281 gcatcttcat agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga
     5341 gccatttcgc cttgttccat agatggtccg tctgccagag tatccccttt ttcgactttc
     5401 tcacccaaat gaacgattgg acgttggttg tagcttgttc cggagtttga acgacggaat
     5461 tttgttacat cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca
     5521 tcaacgtatt caacgacacc gtcatgttta cataacaaag cagctcctga gtcatgcgct
     5581 gatttgtatt ccataccagt accaacccat ggtgcttgag gattgatcaa cggcactgct
     5641 tgacgctgca tgttggcacc catcaaggca cggttggagt catcgttttc caagaaagga
     5701 atacatgctg tcgcaacggc aactacttgt ttaggtgata cgtccatgta gtctacttta
     5761 tcaatagata cttctaagtt ttcactttga gcacgagcca taacaacgtc ttgagcaaat
     5821 gttccatctt cattcaatgg gctgtttgct tgggctacga tgtaatggtc ttcgatatca
     5881 gctgtcaagt agtcgatttg gtcagtaaca cgacctgttt cacggtctac acgacgataa
     5941 ggtgtttcga taaatccgaa tttatttact ttcgcataac tggacaaact attgatcaac
     6001 ccgatgtttg ggccttcagg agtttcgatc ggacacatac ggccatagtg ggagtagtga
     6061 acgtcacgaa cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc taaggctgaa
     6121 agacgacgtt tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac
     6181 tgagaagaac caaagaattc tttgatactt gccacaactg gacggatatt gatcaattgt
     6241 tgaggtgtca atgtttcagt atcttggatc gacattcttt cacgaaccac acgttccata
     6301 cgtgctagcc cgatacggaa ttggttttgt aaaagttcac cgactgaacg gatacgacga
     6361 tttcctaagt ggtcgatatc atcgacattg ccgatacctt ccatcaagtt caagaaatag
     6421 ctcattgaag caatgatgtc ggctggacga accgttttta cagctgcttc tggatagccg
     6481 tttccgatca cgtttacttc acgttcaggg tctcttggtg aaaatacttt gatgacttgg
     6541 acagtcattg gatctgtgac cacaccgtct tctgatggat agtaagtcac tgaatttagg
     6601 ccattatcaa tgaaaggagc tagtgtttcc atgacttggt gagtcagaac tgttcctttt
     6661 tcaacgatga tctcgcctgt ttctggatca actaatgttt cagctaatgt cagattcaat
     6721 agacgtgttt tcaaatctaa ttttttgttt actttgtaac gaccaacgtt tgctaagtcg
     6781 taacgttttg gatcgaagaa acgagcattc agcaagttac gtgagctgtc tgctgttttt
     6841 ggttcgcctg gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg
     6901 cttgcgtttt tatgcaagtc tttttcgact gtattgcgta atgtttcgct gtcgccgaag
     6961 atttcaaaga tcgtatcatc ggaaccaaat cctaacgcac gaactaatac ggttaaagga
     7021 atcttacgtg tacggtcgat acgtacataa gagatgtctt ttgcatcagt ttccatttct
     7081 agccaagctc cacggttagg gataacagta gaaccgaatc cttcttttcc gtttttgtcg
     7141 acttttccat ggaaatagac acctggtgaa cggactaact gagaaacgat tactcgctct
     7201 gccccgttga tgatgaatgt tccttgttct gtcattaatg ggaaatcacc gaagaatact
     7261 tcttgtgctt tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt
     7321 gcagagtagt ttgcatcatg cgcacgagct tctgctactg tatactttgg ttctttcaat
     7381 tcataatcta caaattccaa agatagattg ccattgaagt catcgattgg caaaatgtct
     7441 tcaaacattt ctctaagtcc ctcatctaag aaccattggt aagagtctgt ttgaatttcg
     7501 atcaaatttg gtaattccaa cacttcactg attcttgcga aacttctacg ttcgcgatgt
     7561 tttccgtatt ttactacgtg tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa
     7621 agactacccg atcaattgtt acaataatat ttcaaatatt atttttaggt aacaacagac
     7681 attttttgcg gcttttaggc aaaaaaaaac caaaaataga acattctctt gaaaccttac
     7741 ttcgtgagct tctacttttg tttccttata ttaaagccga aacggacaat atttcgtttc
     7801 tgccttttct tcatcagata gtttttgcaa cttcatatac tacacttatt ttctataaaa
     7861 gtcaacactt cctggcgttt cttctgcttt ttcgcaacat tttcttaatg ttttcaattt
     7921 tatgttttca attatctgac aattcttaat tgacagacct ttttatctcg gttatacttt
     7981 agaaaattca ttttgtggag gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac
     8041 acaactgaat aacttttact ttagctactt tagataggtt tgtattcatc atgatggata
     8101 caaacgttaa acgacagttt gtatctatga tggctaaaga actttgtatt gcagcaaaaa
     8161 gccacgtaga caaaatgcta agtggctgag cagtgaaaat acaaagtaaa agtatcaagc
     8221 attctacttt ttcaatttat tttccgactt ttcaatccat ttggacattt tgtctgaatg
     8281 gattttttgt ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat aatcatcgga
     8341 ttaggcgctg ccctgctact agctttagga ggttgcgcac aaaaagacaa ttccgcagag
     8401 gcatcagatg gacaaaaaac aattggcgta ttgcaacttg tacaacataa ctcgttagat
     8461 gctgcctata aaggattcaa agaaggatta gcagaagaag ggtataaaga aggcgataat
     8521 ctaacaatcg attatcaaaa tgcccaaaac aatcaagaca acctgaaaag tatgagtgag
     8581 aaactcgtca aagaagatcc tgatttgcta cttggaatcg ccactccagc agcacaatct
     8641 ttattaaatg aaacaaccac tatcccgatg actgtcacag cggtcacaga cttggaacaa
     8701 gcccatttag tcacatccaa tgaaaaacct ggcggcaatg tgactggcac gacagatatg
     8761 gtgccaatcg aaaaacaaat cggcttactg ttaaatatcg tgccagacgc caaaacaatc
     8821 ggcatcatgt ataacaacgg tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa
     8881 gaattgaaaa aagcgggcgt caaagtcaaa gtactgacag caaattccac aaatgatgtc
     8941 caacaagtga cgaaaagtct agctaaagat gtagatggaa tctatattcc tactgataac
     9001 acgttcgcaa gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc
     9061 gttgctggcg cagttgatca agtcaaagac ggcggtctag caacagtagg aatcgactac
     9121 gaaagcttgg gtaaacaaac agggatcatg gcagcaaaaa tcttagacgg caagaatcca
     9181 agtgaacttc ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa agacatggcc
     9241 aaagcattag gcatcaatcc agacagcatc aaagaagctg aataaaacac aggaggattc
     9301 tcatgacaaa tttgctctct cttttcttat catctgcatc gcaaggggtc ttatgggccc
     9361 tacttgcgat tggtgtatac gtcactttcc gtatcttgga cattgccgac ttgactgctg
     9421 aaggaagctt tcctttaggt gcagggatcg cggcagtaag tattacaaat ggctatcatc
     9481 cattgacagc ttgtcttcta ggctttttag ggggtgcggc agcagggctt gtttccggac
     9541 tgcttcacac aaaattgaaa attcccgccc ttcttgcagg gatcatcacc atgactggtt
     9601 tatattctgt cacatcccgt gtgatgggtg cgccgaacat ctctttattg ggtcagccaa
     9661 cgatctttca ttgggtcgag tcattcggca taacaaaaga caacgctgta ctgatcgtcg
     9721 gtctgctttt tgctttgatc gttgtatgtt tacttgtttt gttcttacgt acagaaacag
     9781 gacttgctat tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac gggatcaaca
     9841 cagataatat gaagatcatc ggttatatga tttctaatgg atgcattgct ctttctggct
     9901 ccttgcttgc gcaaaacaac ggctttgctg atttaaattc tggtattgga acgatcgtaa
     9961 ttggacttgc ttctattatc atcgcagaag ttttgttacg taaccagcca ctatttctcc
    10021 gtctcttgac tgtcgtttta ggtgcggtca tttatcgttt tattttagcg ttagtcttcg
    10081 aactgaatgt tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc
    10141 tttctttccc gcagatccag caaaaattcc atttacctca aaaaaagaaa ggcggtagtg
    10201 tcaaatgaat gacgtcttaa cattggaaaa tctccatcat acctttgaaa aaggaacgat
    10261 caacgaaaat catgtcctga aaggcattaa tctttcctta gatcccggcg acttcgtgac
    10321 gatcatcggt ggaaatgggg ctggtaaatc tactttgctg aacagcatcg ccggtacatt
    10381 ttctgtcgat cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt
    10441 cgaacgttcg aaatccatca gtcgcgtatt ccaagatcct aaactaggga cagccgttcg
    10501 tttaacggta gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt
    10561 ccgtggtgtc aaaccacaag accgaacatt tttcaaagac cagctggttc ggctaaattt
    10621 ggggttggaa aatcgtttat cctcagaaat cggtttgctg tctggaggac aacgtcaagc
    10681 tattacgtta ttgatggcca cattacagcg tccagaactg atcttattag atgaacacac
    10741 tgcagcgctt gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa agctgatcac
    10801 tgagcagaat ctcactgctt ttatggtcac tcatgacatg gaagacgcga tccgctatgg
    10861 gagtcgactg atcatgctgc atcaaggaag gatcgtcgta gatatccagc aagaggaaaa
    10921 acagcagctc acagtgcccc aattattagc gttgttcaaa caaaacagcg gcacggcatt
    10981 aaaagacgat caagtacttt tatcttcata atcagaaaga ggatgggaca aaaaatgaac
    11041 gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta tcaaccgttg
    11101 tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg gaaaggagct
    11161 tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac cagattttct
    11221 ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt ggaaagccat
    11281 caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt atcgttacct
    11341 gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt ttattcctga
    11401 tttgaaagta caaacgatgt tgacttctga atctacgatg aaagatgcca aactggcagc
    11461 agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac aagctcgtgg
    11521 acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga gtctcttgct
    11581 ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag ccagtgcctg
    11641 tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg tcaatgatat
    11701 ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg atatggaaag
    11761 cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc ctcaaaatca
    11821 ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac aagctagtgt
    11881 cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc tttctcaccc
    11941 agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt
    12001 caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc atggcgaact
    12061 gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca gtctcacatc
    12121 tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat acagtgtcct
    12181 tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt ttctctatcc
    12241 tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt taaatgaatc
    12301 aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca ggccgcttcc
    12361 tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa cttgcgactt
    12421 attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa caaacgtctc
    12481 gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac gagacgcatt
    12541 cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc ggaaggttcc
    12601 agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc tagcagatct
    12661 tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca atcttgttcg
    12721 ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca aattggagaa
    12781 gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca agccagccaa
    12841 gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc ctcctcaata
    12901 gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact ctcgcatagc
    12961 atatctaaat aatccattta ttttcctcct ttttatttat cctacttact ttttcctaat
    13021 tctaggatac ctacttatat gttactacac acccttattt tttacacttt aacagtcacc
    13081 atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga
    13141 agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt tcaaacgaa
//
LOCUS       DADXTG010000069        13117 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.070, whole genome shotgun sequence.
ACCESSION   DADXTG010000069 DADXTG010000000
VERSION     DADXTG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.070"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            152..1093
                     /locus_tag="IWI57_001712"
     CDS             152..1093
                     /locus_tag="IWI57_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBC2449855.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1162..1893
                     /locus_tag="IWI57_001713"
     CDS             1162..1893
                     /locus_tag="IWI57_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBC2449856.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2037..4217
                     /locus_tag="IWI57_001714"
     CDS             2037..4217
                     /locus_tag="IWI57_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBC2449857.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4237..4719
                     /locus_tag="IWI57_001715"
     CDS             4237..4719
                     /locus_tag="IWI57_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBC2449858.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4735..5496
                     /locus_tag="IWI57_001716"
     CDS             4735..5496
                     /locus_tag="IWI57_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBC2449859.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5627..6961
                     /locus_tag="IWI57_001717"
     CDS             5627..6961
                     /locus_tag="IWI57_001717"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBC2449860.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7268..7753
                     /locus_tag="IWI57_001718"
     CDS             7268..7753
                     /locus_tag="IWI57_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBC2449861.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7746..8429
                     /locus_tag="IWI57_001719"
     CDS             7746..8429
                     /locus_tag="IWI57_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBC2449862.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8555..11200
                     /gene="polA"
                     /locus_tag="IWI57_001720"
     CDS             8555..11200
                     /gene="polA"
                     /locus_tag="IWI57_001720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBC2449863.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11245..12081
                     /gene="mutM"
                     /locus_tag="IWI57_001721"
     CDS             11245..12081
                     /gene="mutM"
                     /locus_tag="IWI57_001721"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBC2449864.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12045..12674
                     /locus_tag="IWI57_001722"
     CDS             12045..12674
                     /locus_tag="IWI57_001722"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBC2449865.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12726..13058
                     /locus_tag="IWI57_001723"
     CDS             12726..13058
                     /locus_tag="IWI57_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBC2449866.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4429 a         2319 c         2894 g         3475 t
ORIGIN      
        1 gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac aaaaaacttt
       61 ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc tttatactgt
      121 ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc tttattacaa
      181 ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct tttgcaaatc
      241 tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta ctgtgtctgg
      301 aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc gatttgctga
      361 tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt ttgtaagata
      421 ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc aagtgacagg
      481 tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga cagtggtgat
      541 tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg tacgcccaga
      601 tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag aatttatgga
      661 aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt ctggttggct
      721 tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta cttatggtaa
      781 ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg atgaacggat
      841 cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt ttgacggaac
      901 accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga ttcttgcttc
      961 cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga aacatccgcg
     1021 caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc ggtttacagc
     1081 tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg cgctatacta
     1141 gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga tgtccatggc
     1201 aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca tcagcttgtt
     1261 ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt attaggaaag
     1321 caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga atattttttg
     1381 caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg tggaaaagaa
     1441 acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac tgaaatctcg
     1501 atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg ccccttgtat
     1561 tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa gaaagactgg
     1621 cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga agggaaaaac
     1681 aatacaggta agacaattgt atttggacat acgattacac caatgctcca tggagatatg
     1741 cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg ggctgtattc
     1801 ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga tattgaatat
     1861 cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa ggagctgtga
     1921 ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta gtttaaccca
     1981 ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc attttaatga
     2041 cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca gactatcgtc
     2101 ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt ccttttatcg
     2161 cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt gaaatcgaag
     2221 agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt cggctaatcg
     2281 atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc gtgaaaatgc
     2341 aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa gcaacgattg
     2401 ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca gatggagagg
     2461 tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct gccgaagaag
     2521 cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca aaatttcgga
     2581 cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc aaggatgagc
     2641 aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc gtccataaaa
     2701 tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt ctgaaagtat
     2761 tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata gtcaaaaatc
     2821 ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat aaacgcttta
     2881 ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca gatgagcaag
     2941 caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca ctaaaaggta
     3001 aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca gtcgtagatg
     3061 cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca gcgcaagcaa
     3121 aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa gtagatatgg
     3181 tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct gagcagttga
     3241 aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct tctgtttact
     3301 ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa agaagtgctg
     3361 tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc gatcctaaag
     3421 ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct gaacaattgg
     3481 attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca gcaagcccgc
     3541 agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt gtcatttatc
     3601 gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca cgcttaggac
     3661 caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa aacattttgg
     3721 acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta acttcagtcc
     3781 aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca cgattatccg
     3841 tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat attttagctg
     3901 ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta ttacgtacag
     3961 atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg gtccgtaatg
     4021 tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt gtccatatat
     4081 cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta ggagatatcg
     4141 taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg acgatgcttt
     4201 ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga actgcagcag
     4261 ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca agcaatgttc
     4321 aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca tttagatttt
     4381 aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat caaacatgaa
     4441 ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga tatggatttc
     4501 aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac gaagaaacag
     4561 ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg caagagaaga
     4621 atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg gattgagaca
     4681 aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag gcctttggaa
     4741 aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt agccgcacct
     4801 gaagagggaa attttgagga actgacggca cctacactta ttttagctgg aaactgtctt
     4861 cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga tcaattgttt
     4921 ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga aaagcaagga
     4981 tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct aaaagaagtc
     5041 tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc tggagagaac
     5101 gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa aatcttattg
     5161 atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt atggcaaaat
     5221 gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca cttttctgaa
     5281 gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata tttccatgcg
     5341 ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg tccgaaagga
     5401 aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta caccagaatt
     5461 aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc gaatcctcct
     5521 ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga gcgtgttgaa
     5581 tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg aaaatcaaaa
     5641 taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg ctcttgtttt
     5701 acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct ttacacaaag
     5761 agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg taaaaccagg
     5821 gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc agcgagcaaa
     5881 agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta tccaaaatta
     5941 tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac tgctctctca
     6001 tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag gacatggtga
     6061 tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt tccttgccta
     6121 ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt attatacaag
     6181 tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa aagctatttt
     6241 tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga tttattatta
     6301 tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa cttctggttc
     6361 tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac cagcattcgg
     6421 taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg aaaaactgga
     6481 tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc gattcagtga
     6541 aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg cagagatcaa
     6601 agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg cagttttcca
     6661 accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag cacttgattt
     6721 agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac aaggaaacgt
     6781 taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca aagaaaataa
     6841 tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg ctggagatgt
     6901 tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa atgtactgta
     6961 atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa aaatcttgcc
     7021 aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc tgcggtaata
     7081 agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt attatttgat
     7141 gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc aagggagcag
     7201 aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa actggagggt
     7261 ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg aaatcgagga
     7321 aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc tgtatttggg
     7381 attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa aaacagaata
     7441 tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg caatttcaaa
     7501 gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta ttgaaccggt
     7561 tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata agatgaagga
     7621 tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt tggatgctgt
     7681 cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa gtgaaggtga
     7741 attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat gcaaaagttt
     7801 atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg gctctttggg
     7861 tatttccttt gcaattagca acttttatca ataattttcc actaggcttt gtgggtctgt
     7921 ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag aatccgagct
     7981 tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta gcggttactc
     8041 ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact ttgatgttca
     8101 tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt ggaagagcag
     8161 gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc attttgagaa
     8221 gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca gggattacag
     8281 cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca gcacatagtg
     8341 gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg ttcttggcgt
     8401 ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt tggggctata
     8461 ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt ctttgttaga
     8521 atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat tattactcgt
     8581 tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc ttgaacgttt
     8641 caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga tgtttgaaaa
     8701 tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag gaaaaacgac
     8761 ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac ctggcgaatt
     8821 caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac agtattacga
     8881 attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag tagacaaaga
     8941 tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag ctagtaaaga
     9001 agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata caccagaaca
     9061 tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga agggactagc
     9121 cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa cagctatcaa
     9181 gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg aaatgaagca
     9241 aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat cgaaaaaatt
     9301 ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga agtatgaagg
     9361 gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc agttcctttc
     9421 taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg aagtcgttca
     9481 tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa tgatgggcga
     9541 caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga aaatttatgt
     9601 cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca ctgatccgac
     9661 tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc gctatactgg
     9721 aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg atacgaatga
     9781 aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata tccaatcaga
     9841 tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg aactcttttt
     9901 tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat tgagccaaga
     9961 actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat tgtctatcat
    10021 tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac aagaaatgaa
    10081 gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag aagccggcga
    10141 agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa aaatgggatt
    10201 acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac tggaacagct
    10261 tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg caaaaattca
    10321 atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag tgcatacccg
    10381 ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta atttgcagaa
    10441 catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc ctagagaaaa
    10501 agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac ttgcgcatat
    10561 ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc atgccagcac
    10621 agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata tgcgtaggca
    10681 ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac tttcacaaaa
    10741 tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg aaaaataccc
    10801 aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag gctacgtcga
    10861 aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt acaatatccg
    10921 ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg ctgatatttt
    10981 gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc aagctacgat
    11041 gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac ttgaatcatt
    11101 agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac cactgatcac
    11161 tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta agacagagca
    11221 atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac cgttcgtaaa
    11281 ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt gtggcccaag
    11341 atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga aaccatccaa
    11401 tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga actgatctca
    11461 cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat cgataaacat
    11521 acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga tgtacgaaaa
    11581 tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg aatcatgaaa
    11641 ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa tggactgaaa
    11701 aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac tggtctaggt
    11761 aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca accggcgaac
    11821 acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt tttggatcgt
    11881 gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg agaatcaggc
    11941 ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat acgttgtgga
    12001 acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc gaattgtcaa
    12061 aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata gctaccggta
    12121 aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat ggcgatgtga
    12181 ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta tcagtgtttg
    12241 gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg atgattgttt
    12301 ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt atccgaaaag
    12361 cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt gtagatatcc
    12421 cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc gtttatacta
    12481 cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgctg gaacaagcgc
    12541 agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg gatattttgt
    12601 tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg ctttttgcta
    12661 atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga aagtgaggga
    12721 tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca aaagaaaaaa
    12781 tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa tcagaaacag
    12841 cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac cagcaggcag
    12901 aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca cctgatcgta
    12961 gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc gaggaacagc
    13021 gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa tcgagatgtg
    13081 acttgaagca gatgagaagg tgttcatctg cttcaag
//
LOCUS       DADXTG010000070        12895 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.071, whole genome shotgun sequence.
ACCESSION   DADXTG010000070 DADXTG010000000
VERSION     DADXTG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.071"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            26..1066
                     /locus_tag="IWI57_001724"
     CDS             26..1066
                     /locus_tag="IWI57_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBC2449867.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            1185..1352
                     /locus_tag="IWI57_001725"
     CDS             1185..1352
                     /locus_tag="IWI57_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBC2449868.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(1412..1765)
                     /locus_tag="IWI57_001726"
     CDS             complement(1412..1765)
                     /locus_tag="IWI57_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449869.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            2270..2692
                     /locus_tag="IWI57_001727"
     CDS             2270..2692
                     /locus_tag="IWI57_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2449870.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            2689..2919
                     /locus_tag="IWI57_001728"
     CDS             2689..2919
                     /locus_tag="IWI57_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449871.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            3380..3583
                     /locus_tag="IWI57_001729"
     CDS             3380..3583
                     /locus_tag="IWI57_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBC2449872.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            3665..4882
                     /locus_tag="IWI57_001730"
     CDS             3665..4882
                     /locus_tag="IWI57_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2449873.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            5114..5440
                     /locus_tag="IWI57_001731"
     CDS             5114..5440
                     /locus_tag="IWI57_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBC2449874.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            5508..6221
                     /locus_tag="IWI57_001732"
     CDS             5508..6221
                     /locus_tag="IWI57_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBC2449875.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            6531..7382
                     /locus_tag="IWI57_001733"
     CDS             6531..7382
                     /locus_tag="IWI57_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2449876.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            7503..9368
                     /locus_tag="IWI57_001734"
     CDS             7503..9368
                     /locus_tag="IWI57_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2449877.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9416..10825
                     /locus_tag="IWI57_001735"
     CDS             9416..10825
                     /locus_tag="IWI57_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBC2449878.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            10933..11634
                     /locus_tag="IWI57_001736"
     CDS             10933..11634
                     /locus_tag="IWI57_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2449879.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11793..12272
                     /locus_tag="IWI57_001737"
                     /pseudo
     CDS             11793..12272
                     /locus_tag="IWI57_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4356 a         2114 c         2752 g         3673 t
ORIGIN      
        1 aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat
       61 tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag
      121 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
      181 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
      241 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
      301 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
      361 aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt
      421 aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct gggcttccat
      481 tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga gctcggtttc
      541 tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac gctatggttg
      601 tgaacaagga ttgtatggtt ggaatgtgac ggactgtaaa atctgtttta agtatggctt
      661 atactatagc cctgttagta ccccagcaga ttttcggatg cttgctccta ttgtattgga
      721 acaagtctta aaaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaaattta
      781 tgcgccacag gaatatcttt cacgagcata caacgatgct cctaaatatt gtgcgaacat
      841 cgtagacact caattgaaaa ataatgaggt cattcttagt ggagaaatcc ctgctcggtg
      901 tattcaagaa tatcgtagtg atttaacttt ctttacaaat ggacgtagtg tttgtttaac
      961 agagttaaaa gggtaccatg ttactaccgg tgaacctgtt tgccagcccc gtcgtccaaa
     1021 tagtcggata gataaagtac gatatatgtt caataaaata acttagtgta ttttatgttg
     1081 ttatataaat atggtttctt gttaaataag atgaaatatt ttttaataaa gatttgaatt
     1141 aaagtgtaaa ggaggagata gttattataa actacaagtg gatattgtgt cctgtatgtg
     1201 gaaataaaac acgattaaag ataagggaag atactgaatt aaaaaaattc cccctctatt
     1261 gtccgaaatg cagacaagaa aatttaattg aaataaagca gttcaaagta actgtgatta
     1321 cagagccaga cgcaaagacg cagagccgat aaaatgagat taatacaatc tcattttatc
     1381 ggctctttcc gttatgtatg gattctttta attagtcttc gatgtttctt gcttcgttga
     1441 taccgctggc taaagattcc attatggata gttctttgtc tgtaaagcta tccatgtatt
     1501 tctctatctg taatcgtcgg gtgcttttta ccaagttatt agcaggtaag aaaaattcat
     1561 caacggaaac atgaagtaac gatacaaggt cataaagaac ttgtatgctg gggtgttgcc
     1621 ctttattttc aatattagtt aagtaccgtg ggtcaatttc aatcaatgct cccacttgtt
     1681 cacgagttaa acctcgtttc aatcgagctt ctttaatggc taaaccaaag gctctaaaat
     1741 catatttatc ttctttttta cgcatagtag accacctcta tacattttat tgttcctact
     1801 gaattaaaaa caggtataga aaaacgtgtt atatggttta taggtttata tttaataaaa
     1861 agcactacta aacgccaata aaaaaaaccg ttatatggta gtgctattta cgctgttaaa
     1921 atattgtata ttacttccaa atggcggttt gttggaggtc aacgtcgcca tgaagtacat
     1981 catatacaat aaatttcctt acattgggtt cttgtcaaaa aaagtcgtct atctgcaata
     2041 gataagtacg tccaccaatg tggttttata aatcatatag atagaataac agaagcatgt
     2101 aaacagagaa ataaatctgt ttatatgctt ttttggctat tcagaacttt tttacaaagt
     2161 ttatttatca gtaatgcaac aaatccccct ttcacattgg gactaagagt gaaaggagat
     2221 aaacgagcaa ggctcacttc ctttcctaga cagaaagggg gtgagaaaca tgaaaccatc
     2281 ttcttttcag accacaatag aaaatcagtt tgactatatc tgtaaacgtg ctatggaaga
     2341 cgagcgaaag aattatatgc tttatctttc aaggattgca aagcgtgagg tgtccttttc
     2401 ggatgttggc gattatcttg ttagccagtt tgcgacaaca gataactatt caactgactt
     2461 tcagattttt acactcaatg ggttatcagt aggcgttgaa aatgatttgt tgagtgaagc
     2521 attacgtgag ttgccagaca agaaacgtga aattctactg ctgttttact ttatggacat
     2581 gagcgattca gaaattgcag acctgttgaa attgaaccgt tctactgtct atcggcatag
     2641 aaccagtgga ctagccttaa ttaaaaagtt tatggaggaa tttgaagaat gaaaacacaa
     2701 tatcctatga ttccctttcc tctcattgta aaggcaacag atggcgatac cgaagcgatt
     2761 aaccagattc tacatcatta cagagggtac ataacgaagc gttccctacg acttatgaaa
     2821 gatgaatatg gcaatcaaag tatggtcgtt gatgaagtct tacgtggaag aatggaaacc
     2881 agactgatta caaagatttt gtcatttgaa attaagtaat atcctctctc ctttcgtgga
     2941 agcgtgctaa accattccac gcttcccgaa cagggaggtt tgttattcca ccaaagcata
     3001 ttgagctttc aatgtgtttt gataggctaa cgagccattg ttctttgaaa actgaataaa
     3061 agtaatcgaa tacgtttcga taagaaaaga gccaacggaa ctaaccgcca tgacctatct
     3121 tataaagata gcgagcgatt catgttagtg atccgagaag caatctttag caggattgcc
     3181 tgcaacgaca ttcttatcgt gataatgata ctcccataca gtcaatagtc cgagcgtgat
     3241 aaaaccgtcg caggcaatga gtatggctac atgagaacca tgcaggggtg gaactcccgt
     3301 gagctttgct aaagctgttc gattgctggt aaaacaactt ttatgaaatc caaataagtg
     3361 atttggaaag gaggatttta tgaagcagac tgacattcct atttgggaac gttataccct
     3421 aaccattgaa gaagcgtcaa aatattttcg tattggcgaa aacaagctac gacgcttggc
     3481 agaggaaaat aaaaatgcaa attggctgat tatgaatggc aatcgtattc agattaaacg
     3541 aaaacaattt gaaaaaatta tagatacatt ggacgcaatc tagcgtcgcc aaagggtctt
     3601 gtatatgata aaatagtatt aagtcgtatc aaggctcttt ccataaagga aaggagcaaa
     3661 tgccatgtca gaaaaaagac gtgacaatag aggtcgaatc ttaaagactg gagagagcca
     3721 acgaaaagac ggaagatact tatacaaata tatagattca tttggagaac cgcaatttgt
     3781 ttactcgtgg aaacttgtgg ctacagaccg agtaccagca ggaaagcgtg attgtatctc
     3841 acttagagag aaaatcgcag agttacagaa agacattcat gatggtattg atgttgtagg
     3901 aaagaaaatg acactctgcc agctttacgc aaaacagaac gctcaaagac caaaggttag
     3961 aaaaaacact gaaactggac gcaaatatct tatggatatt ttgaagaaag acaagttagg
     4021 tgtaagaagc attgacagta ttaagccatc agacgctaaa gaatgggcta ttagaatgag
     4081 tgaaaatggt tatgcttatc aaaccatcaa taactacaaa cgttctttaa aggcttcatt
     4141 ctatattgct atacaagatg attgtgttcg gaagaatcca tttgactttc aactgaaagc
     4201 agttcttgat gatgatactg tccctaagac cgtactaaca gaagaacagg aagaaaaact
     4261 gttagccttt gcaaaagctg ataaaaccta cagcaaaaat tatgatgaaa ttctgatact
     4321 cttaaaaaca ggtcttcgta tttcagagtt tggtggtttg acacttccag atttagattt
     4381 tgagaatcgt cttgtcaata tagaccatca gctattgaga gatactgaaa ttgggtacta
     4441 cattgaaaca ccaaagacca aaagtggcga acgtcaagtt cctatggttg aagaagccta
     4501 tcaagcattt aagcgagtgt tagcgaatcg aaagaatgat aagcgtgttg agattgatgg
     4561 atatagtgat ttcctctttc ttaatagaaa gaactatcca aaagtggcaa gtgattacaa
     4621 cggcatgatg aaaggtcttg ttaagaaata caataagtat aacgaggata aattgccaca
     4681 catcactcca catagtttgc gacatacatt ctgtaccaac tatgcaaatg caggaatgaa
     4741 tccaaaggca ttacagtaca ttatgggaca tgctaatata gccatgacgc tgaactatta
     4801 cgcacatgca acattcgatt ctgcaatggc agaaatgaaa cgcttgaata aagagaagca
     4861 acaggagcgt cttgttgctt agtagtacaa atgaatttac tacttattta ccacttctga
     4921 cagctaagac atgaggaaat atgcaaagaa acgtgaagta tcttcctaca gtaaaaatac
     4981 tcgaaagcac atagaataag gctttacgag catttaagaa aatataaaaa gataattaga
     5041 aatttatact ttgttttatg taaaaaaatg gtatgattaa tcaagtaaga agtgattgga
     5101 agaaggcaag caaatgaata gtatggaaaa agtcaaccag tttagagatg aacgtaattg
     5161 gcgacaattt cataacgaaa aagatttggc tatctctatt tctttggaag catctgaatt
     5221 gttagaactt tttcaatgga aaacgccgga agaagtaaaa gaaacacaat tagaacgaat
     5281 caaagaagaa ctggcagatg tattgatcta ttcatacatg atggcagaca accttcacct
     5341 ggatctagat gagatcattg aagaaaaatt gatcaaaaat aatttgaaat atcctgtaga
     5401 aaaaagcgca ggtgtacgaa aaaaatacac agagttgtag aaatgtgtca ttcggctgtt
     5461 ggaaagagca aaagaaaaac agtaaaatga gtctggaggg agagaaaatg aaaaaaaaag
     5521 gaaaagcaaa aagatggcta gtcaatatcc tcttgttcct cttgttatta gtaggactag
     5581 ctttgatttt caatgaacag atcaaggatt attttgtcag agagacagga gataaatatg
     5641 ctatagcgaa tgtaacaaaa gaagatctga aaaagaataa cgataaagat gtcagttttg
     5701 actttgatgc ggtagaacca atgacgacag aaggggtcat gaggtcgcaa atgagaggaa
     5761 cagatctacc agtgattgct agtatcgcag tcccttcagt ttcaatcaat ttaccggttt
     5821 tcaaaggctt ggataatacg tcactacttt acggagcagg aacattatct ccagatcaag
     5881 aaatgggaaa aggaaattat gccttagcaa gccatcgtgc aacgaaccct gaattgctgt
     5941 ttacaccgct agaaaattta gagatgggtg ccaagatcta tttgacagat ttagaaaatg
     6001 tgtatacata caaaacattt tttaaagaaa aagttgctcc tacagatacc caattactga
     6061 atgaagtcga gggaaaagaa attgtcacac taattacttg tggcgacatg gacgccgtca
     6121 cgcgactagt tgtacaagga gaactggaaa gtgttacatc gataaaagat gcgacagatg
     6181 atatgagatc tgctttcaat ctagagacaa aaacatttta ggatactggg acataagtcg
     6241 ttacagatta aaaacagcca aaggatatga atcgataagg aaaaaccaat ttgctgaaaa
     6301 aaagagaaac gcaaagaggt gcatctctga tttggttgtt ttctagatag acttgtgttc
     6361 cagttttttt attttttata tgaaaaggct tgcataaata aaaattcgat ggtatgatga
     6421 ttttgaataa gaaatggatt gttactgtac ggcaggcaaa accagaattc agctaagaaa
     6481 cagtaggcga attctaggtt tttttatttt tcttgagagg gcgaagcaaa atgaaaattg
     6541 ataaaatttt gaataacaat gtcgtcattt cgaaaaatgg attcggggaa gaagtcgttt
     6601 gtatgggaaa aggactagct tttcaaaaaa agattgggga cgagatttcg ccagaagcgg
     6661 tccaaaaaga atttgtttta agagattctt ttgctaaaaa ccaattccag cagctgatgg
     6721 ctgatattcc ggcagaggag atcgaatggg tcaaacaagt agtcgaactg gctgaagata
     6781 aactaaaagt tgaattttcg cctaatattt atctgacatt gaccgatcat cttcattatg
     6841 cgatcacacg agcaaaagaa aatatccaac tgcctaatcc attattattt gaaacaaaaa
     6901 aattttatcc gaaagaatat caagcagcga aagaggctct tttgttcgtg aaacaaaaga
     6961 cgggtgtgga actttctgag gatgaagccg gttttattgc ctttcatttt gtgaatagcc
     7021 aacaaggaaa cgaagacatg caagtgacga tgaccgctac cacaatggta agagatatac
     7081 tgagtatcat cagtaaattc ttcggcatcg tgttcgatga agaatctttg aattatcagc
     7141 gtatcattac tcatctgcag ttttttgccc agcgttatct caaaggggaa ggatctgacg
     7201 agcaagatga atttctctac gcattagttc aaggaaagta tccaaaagcg tttcattgtg
     7261 tcgaacggat caatgagtat cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc
     7321 agatttattt aacgattcat attcaaagag tagtaagtga gaaaaaaagc atccaatcat
     7381 aaggattgtt actgtatggc aggcaaaacc tggattgaaa aaataggttc acgaatgttt
     7441 gtgactgttt ttttcaatct gggtttttat tttttaaata aaaaagaaag aaggaaaaaa
     7501 caatggataa tcaagcagtt ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg
     7561 tcaaaagttt agtacattgt gctacgcggt tgcgattcag attaaaagat gaatctttag
     7621 cagatacgca aaagttaaaa gaagacccgg acgtgatcca agtcgtacaa agtggcggtc
     7681 agtatcaagt agtgattggc agtaatgtag cagatgttta tcaatcgatc gtggatgagg
     7741 aaggtcttgc agtttcggag gatggaaaag aagagtccaa aaatattttg aatcgtctga
     7801 tcgatattat ctctagtata tttacacctt ttctaggggc gatggcagcc gccggggtat
     7861 tgaaaggatt cttatcattg gcaacggtga tgggctggct gacagctgat agcggtactt
     7921 atcaaatatt gtttgctgca gcagatggtg tctttacctt cttacctgtc atgcttgctt
     7981 ttacagcagc taaaaagttc aaagccaacc agtttttagc agtagcaatt gctatggcac
     8041 ttgtgtatcc agctatcaca gcagttgctg gtgcgggaga agctatcagt ttctttggga
     8101 tcccagtgat tctttcacca tctggttata cttcttctgt cataccaatc attctagccg
     8161 tatgggttca aagtaaatta gagccattcg taaaaaaagt gattccgcaa tttctgcaaa
     8221 tgattttagt tccgcttgtc gttttagtag tgatggttcc attaacattt cttgctttag
     8281 gaccaatcgg gacggttgcc ggtaatgctt taggcggtct cttcaactcg atttatggat
     8341 ttagtccaat cgtagcaggt ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg
     8401 gcatgcattg gggattcgta ccgatcatgt ttttgaatat cgaacaatat gggtttgatg
     8461 tattgatgcc aatgcttctt ccagccatct tggcacaagg tggggctgct ttagctgtag
     8521 cattgcgaac aaaagatacg aaacttcgag cattagggat ttcttctaca gtgacttcgt
     8581 tatttggaat cactgaacca actgtgtatg gtgtgacatt acctttgaag aaaccattca
     8641 ttgccgcttg tatttctgga ggtattggtg gagctattat cggattttcc ggagtaaaag
     8701 ctttctccag cagtttggtc agcttattga cgattccgac tttcattaat acggtggatg
     8761 gtgtagagtc aaatgtgaca gtagcagtaa tagctacagg aattgctttt gttcttgcat
     8821 tcgtaggtac attgatttta ggatttgacg aacaaacaca agacaatcaa ttagaaaaca
     8881 aacatgcgaa tgcaggagaa ccgattactt cagcacggca taccttaaaa agtcctttaa
     8941 cgggtaaagt actgccattg agtgaagtac cagaccaagt tttttcttct ggcgtgatgg
     9001 gaaaaggtat cgctattgat ccagaagtag gagaactagt tgcacctgca gacggagaga
     9061 tcacgactat ttttccaact ggacatgctg tagggatcac aacgactgac ggagcggaga
     9121 ttttgattca tatcgggatg gatacagtag aattaaatgg gaatggcttt gagatcttag
     9181 tcaaacaagg ggatctcgtc aaagcaggag atttattgat tcgttttgac atcgaagcaa
     9241 ttagagcagc tggctatagc gtaatcactc cagttgtcat tacgaatact gatgcatttg
     9301 ccgatatact tgaattagac caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg
     9361 taaaataagg aggaaatcag gatgcaatca cgtttttcag aaaaattttt atggggtgga
     9421 gcaactgctg ccaaatcaat tagaaggggc ttatgatcga gacggaaaag gactatccgt
     9481 tgcagatgct atgcctggag gtaagcagcg ctttgcgatt atcggcagtg aagaatttga
     9541 ctggacgatt gatcaagaaa aatatattta cccgaatcat cgaggaatcg atcattacga
     9601 tcgtttcaaa gaagatcttg ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc
     9661 gattgcctgg acacgaattt ttccaaatgg agacgaaggt actcctaacg aagcgggatt
     9721 agaattttat gatcagctga ttgatgagtg cttaaaatat gatattgaac ctgtgatcac
     9781 gatttctcat tacgaaatgc ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg
     9841 aaaactgatc gacttctatg aacgatttgc tcagacagta ttagagagat acagcagtaa
     9901 agtcaaatat tggatgactt tcaatgaaat caattcggcc tttcactttc cagctttaag
     9961 tcaaggatta gtaaaaagca atggagcagg agagtaccaa aatattttcc aagcatggca
    10021 taatcaattc gttgcaagta gcaaagccgt aaaaatcggc cacgaattgc gttcggatat
    10081 ccaaatcggc tgcatgatta tctatgcaac aacttacagc atcgatgcca atccagtcaa
    10141 tcaggcagca acaatgatcc aaaaccaaga attcaatttt ttctgcacgg atgtccaagt
    10201 acgtggagaa taccctgcat acacagcgcg tacccataaa aaatatggag tcgatccgga
    10261 aaaattagag caaacggaaa aagacttcca gttgctaaaa gaatatccag tggactacat
    10321 cggatttagt tattatatgt ctacggcaat caatgagact gatccacaag cagcaacttc
    10381 agaagggaac ttgctgggag gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg
    10441 gcagatcgat ccagaagggc tgagaatcgc attgaatgaa ttatacaatc gttaccagaa
    10501 accattattc attgttgaaa acggtcttgg agccattgat catatggaag aggatggcac
    10561 gattattgat gattatcgga tcgattattt acgtcgtcat atcgaagcga tggctaatgc
    10621 agtagcagac ggtgtagatt tgatgggcta tacgccttgg ggatgtatcg atcttgtcag
    10681 tgcatcaaca ggagaaatga gtaaacgtta cggctttatc tatgtggatc tagacgatga
    10741 aggaaatggg acactgaatc gatcaaagaa aaaatctttt gattggtata aagaagtaat
    10801 tgcctcaaat ggcgtaaact tgtaattaaa gcagctgaat gaaaaagaac acacttctca
    10861 catgtgattt gtgttctttt tttatttttt atgtgctatt cttttagtaa tagtaaagca
    10921 aagggtgacg ttatgaaaag gacacgtgta ctatacttgg aagtcgcaga taaaattaaa
    10981 gaagatattt tctcaggaaa atatccagtc ggttcgatgt tgccgacaga aagtgaatta
    11041 gaagaactgt ttcaagtcag caaaatcact gtccgaaagg caatcgaact gctggcatca
    11101 gatgaatatg tcgcaaagaa aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac
    11161 aatcgattat ccaaagcggc aagctttacg caaatcttgg agaactcccg atatgaagtc
    11221 cggaaagaaa cgctggattt gcaattggtc catataaaga aggaagatcc tttgttttcc
    11281 tttttcggtg agcaagctgt ttgttttaag cgcttatatt atttgaacaa tcagccttat
    11341 atttattttg ttcattatct tccacaagaa atgatgcaat tcagcagaga agaatttggc
    11401 aaagaatctt tgtatcgttt gctgacgaaa aaaggatata ctattgactc attttctgat
    11461 gatttctcgg cagtattttt aacagaagaa gaacaaggaa ttctgaaaac agacgcacag
    11521 atcggcatca aacggatacg gaaatcgtct acgcaaattg gggaaatgat tgaatattcg
    11581 gaggcggtct accatacgga gctgcatcct taccatattg attatgaaac ataaataaaa
    11641 aaagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    11701 ttctcatatt tttcgtttgg ttaggcttat taccgcagat acacggctgt ttctggatta
    11761 ccatttattc tggataaggg ggtgtggcac gattttcaga aggaatctat gaaccaatca
    11821 ctgtattgat tttagcttcg atggtaaccc cattcggctt gacgatcgct tatttcttag
    11881 ggaaaatcat tcgtaaaaat atactcaatc gtcaagaaat cgatacattg aagaccgctt
    11941 ttcctatggg gatctgtcag atcacagagg gatgtttccc tatcgtattg aacgacttgg
    12001 cacgcaatgt catagcaacg ggagtcggtg ggacagtagg tggcggatta agcatgtttt
    12061 ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc agtcgctacg atgaccagac
    12121 catgggcatt cattggtgca ttgttagctg gttcttttgt cacaggaata accattttat
    12181 tattgaaaaa accagtagat ccgaatgcag aaatcatcca agaagaaaaa gaggaagaag
    12241 acatatcttg ggatgatttg acaattagtt aggagaggca gaatggatta caagaaaaaa
    12301 gtacaataga tgtaccttga gacaggaatg gtatgaaaga atcagagtta gaaagaatcg
    12361 aatatacaga ttttggttta ggagcgatcg aaactgtcca gagcatcggc accctccttg
    12421 aaatgacgat gaaggaaaaa tgagaacctt tcgttgtcga aagggtctgg tataccttat
    12481 gtcgaaggaa aggaaacaaa aaaactttcc tgtactattc caaaaaggaa agaaagttat
    12541 tatacagtaa aaaaagaaat tattcttcat gctagagatc agtacacgtt cgagcccaat
    12601 atcaaacact agttccaggc aggcgcaaca ggtgcggtta tcactgaatt ttcttccagc
    12661 agtgacgatt caagtgatat ttttacaaat ccagctattt atcgttaaca aaaagatttt
    12721 taggagaagc tgatgaacaa aaatgatcaa attttgatcg tttagttcgt cggctttttt
    12781 atttagagaa acatcattta tcagtgaaac ataacacgag aacaaaatga ttttttcgtt
    12841 aataaaagaa acaagtattt gttgtttaaa ataatacacg ttattggcat atagg
//
LOCUS       DADXTG010000071        12813 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.072, whole genome shotgun sequence.
ACCESSION   DADXTG010000071 DADXTG010000000
VERSION     DADXTG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.072"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            141..623
                     /locus_tag="IWI57_001738"
     CDS             141..623
                     /locus_tag="IWI57_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449880.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            820..1710
                     /locus_tag="IWI57_001739"
     CDS             820..1710
                     /locus_tag="IWI57_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBC2449881.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1997..3271)
                     /locus_tag="IWI57_001740"
     CDS             complement(1997..3271)
                     /locus_tag="IWI57_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2449882.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            3544..6015
                     /locus_tag="IWI57_001741"
     CDS             3544..6015
                     /locus_tag="IWI57_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBC2449883.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6118..7854)
                     /locus_tag="IWI57_001742"
     CDS             complement(6118..7854)
                     /locus_tag="IWI57_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBC2449884.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7851..8555)
                     /locus_tag="IWI57_001743"
     CDS             complement(7851..8555)
                     /locus_tag="IWI57_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2449885.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8686..9189
                     /locus_tag="IWI57_001744"
     CDS             8686..9189
                     /locus_tag="IWI57_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBC2449886.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9149..9487
                     /locus_tag="IWI57_001745"
     CDS             9149..9487
                     /locus_tag="IWI57_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBC2449887.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9508..10926
                     /gene="ffh"
                     /locus_tag="IWI57_001746"
     CDS             9508..10926
                     /gene="ffh"
                     /locus_tag="IWI57_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBC2449888.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11038..11616
                     /locus_tag="IWI57_001747"
     CDS             11038..11616
                     /locus_tag="IWI57_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBC2449889.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11620..12141
                     /locus_tag="IWI57_001748"
     CDS             11620..12141
                     /locus_tag="IWI57_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2449890.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12220..12774)
                     /locus_tag="IWI57_001749"
     CDS             complement(12220..12774)
                     /locus_tag="IWI57_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBC2449891.1"
                     /translation="MTKKIGFFIGILRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         4109 a         2347 c         2650 g         3707 t
ORIGIN      
        1 caattacttt ttcattattt ctagattaaa tgaataaact ctcatgccat tatctctaaa
       61 aaaaaagttt ttccccctaa tttttttaga tagaaaattg tatagtatga tgagagaaaa
      121 agtagtttgg gggaacgtta atgagaagaa aagcagtaat cgatcggctt tatttatttt
      181 tttggttttt tcctatcgtt ttgctacctt ttacatgggt aaatgctgtt gtacaaggtg
      241 cggagatatt aggcctgatc caaggaagcg gatttgtggt ttctaggaat tttccagctt
      301 tgattgtttt ctatttagtg ataagtgtag gtctcttatt accaaaatct ctgatcaatc
      361 actacaattg gcagctgctt tacttttctt gcttattagt atttacatct atgttttctt
      421 tgctgcctcg cttgattatc ggccctaatg gtttgagcga gagtggattg aaggatttag
      481 cttattcctc catgtatttc ttgacgatca aaccatggtt ctatttagta attgcctcat
      541 ttattcttgc aacatttttt tacttctgga cagaaatcag gataaaaagt gaacgatctg
      601 tatctataaa aaataactat tgatggtgga tcatcagtaa aaaaacatat gagctgcgta
      661 aaatgcatgt ttctcatatg ctttttttat tattatgtaa agaatgcgaa aaacgtttac
      721 atgattttta cataacttaa ctatattttt acagcaacca tacgagggat ttacagtcga
      781 ctgataaact ttagttgtaa ataagaaagg agcaataaaa tgaaaaaagt attgtttact
      841 ctaggtatga tgggaattct tttagccgga tgcggctcag gatcaggaaa tacagatggc
      901 tctgccacac aaaataattc aagtgactca aatgaacaag taaaaatcgt tgcggtaggt
      961 tcgacagcat tacagccgtt agtcgatgca gcacaagaaa gctttgtaca agaaaatcca
     1021 aattatcaaa tctctgtaca aggaggagga agcggaactg gtttgagcca agtcgaagct
     1081 ggagcagtta ctatcggaaa ctctgatgta tttgcagaag aaagagacgg agttgatgct
     1141 tcaaaattag tggatcataa agtagcagtg gtcgggatgg caccaatcgt caataaagat
     1201 acagatgtca aagatatcac caaacaggaa ttgattgata tctttacagg aaaaatcact
     1261 aactggaagg aagttggcgg gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc
     1321 agcggtaccc gcgcgacatt cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc
     1381 caagaacaag attcatcagg aacagttcgg cagctggtaa gtcaaacacc aggagcaatc
     1441 agctatttag ctttctcata tctcgatgat tccacacaag cactaagtat cgacggtgta
     1501 gaaccaaaag aagagaatgt agcagataac agctggggaa tctggtcata tgaacatatg
     1561 tacacgaacg gaaaaccttc ccctgaagtc caaaaattct tagactacat gatgacagaa
     1621 gaaatccaag aaggacctgt caaagaattg ggctatttac caatcacaat gatggaagta
     1681 gaacgtgatc acgaaggaaa tatcaaataa ctagcagata aacatgccac ggtagtcgtt
     1741 actattaagg aaaaatcaac tatgtttccc cacatataca tttttccttt aattgtgaaa
     1801 aatagttcct catattttgc cgttttttga gctaataccg agggtcagct gattgaacac
     1861 cgtttattcg aaaaaaagag ctacgacata gaactgtcgt aacttttttt ggctcttcgt
     1921 caaatcgtgt tggtgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg
     1981 tgcgccgcat atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc
     2041 gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat
     2101 tcagcccctc agtgacgccg tttgagtggg acaccttaaa ggcatttgaa atacccgatt
     2161 ggtagcaatg gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg
     2221 ttttaagggt ttcttgaaag ccttccgtac ctcgtttaac gaaataatag cgtaataatt
     2281 gacaaaaatg atacgcagtt cgcaattctt cgttgtagct taacagctca tcaacgattt
     2341 ctgtttgact aattggtcgt ttaaacagtc gatgataatg ccgtttgagt ggctcccatg
     2401 tcgtggaatc tttgagtaag agtttccaat atcgcttgag ccgacggtac ttcttctgat
     2461 tttcagagtg tgaggttcga aaacgattca tgatctggac acgtaattga ttgaagctgc
     2521 gattgatgtg ttggacaata tgaaagcggt cagttacgat ctccgcattt ggaaagactg
     2581 ttttaagcaa ctgacaatag ctggcattca tgtccatcac taggtacttg acctttaaac
     2641 gggcctttct aggaaagcgc aggaagtatt gagttagttt gtaaagtctg cgatcttcaa
     2701 ggatcgtaaa tagctgattg gttgtcccat caacacaaat aaaactcatg gaaccagaaa
     2761 cagaattcat tgatttgaac tcatccatac acaacaccgt cggaaggaag ttcatacgtg
     2821 gctggtaatg cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc gagacgaagt
     2881 acttcttagc gattagttta cgtgattgat tttcgtacag ttccatgaga atcgcctgct
     2941 tcagttcctt tgttagttga tgatgttcat caactaagtc cgttttcgca gaaaaggtcc
     3001 ttccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtt aacttttctt
     3061 tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt gcggataata cgaaccgatc
     3121 gacagtgttc acaataggaa gaagagtcat ccaattttcc tttaataaac tgcgcttgaa
     3181 cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta
     3241 atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag gatgacgttt
     3301 tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaagtgt
     3361 tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaacc cttttttttt
     3421 gctatatgac aggaatgaaa ggtgtttgca agcttaatgt gaggaacact ttccatatct
     3481 gttctatact ggataggaga agtttggaaa tatccagaaa acgaaatcaa tcgaggtagc
     3541 cacatgaaga ataaatggat aaatcgaagt aaaaaaaaga aagtaacaca aagatcatca
     3601 gataaagtca tccgtctttt tccatcgtat aaagaaggat taacggcaga tcaagtcaag
     3661 gaacgaatag aaaaaggtgc agcaaataat tcagtcgatc ccacattcaa aacaaatcag
     3721 caaatcgtat tggaaaacat atttacgtat ttcaatttga tctttcttat cttagccatt
     3781 ttgctttgtt tagtagaatc ctataagaac ttgacttttt taccagttat catagccaat
     3841 acaggaatcg ccatttttca ggaaattcga tccaaaaaga tcttggacga actaaatgtt
     3901 ttaaatacag caaaagtcaa tgtcatacga gacgggatag aacaaacaat agatatagaa
     3961 gaccttgtaa tagatgacat tgttatttta gagactggat atcaaattcc agcagatgca
     4021 gaggtcgtgg aagggaaact gcaagttaat gaggctttat tgacaggcga agcagatgaa
     4081 ataaccaaag aaataggtga tccattaatg tccggcagtt tcattgtttc cggaaaagct
     4141 tacgcccgat tagacaaagt tggagcagat tcgtatattt cccagctgac agtcaaagcc
     4201 aagacaatgg gagaaggaga acagtcggag atgattgctt ctcttaatca attgatcaaa
     4261 tggattggga tcatcattat tcctatcggt atcctgcttt tttctcaaag ttactttttt
     4321 aatggaaaca cgatcaaaga aagtgtcgtt gcaatggaag cagcattgat cggtatgata
     4381 cctgaaggac tgtacttgct gacgacgatt gcattagcat taagtgcgac taaactagca
     4441 aagcaaagag ttcttttaca caatatgaaa agcattgaga cattggcaag ggtaaatgtc
     4501 ctttgtgtag ataaaacagg aacgatcaca gaaaataaaa tgtctgttca aaaaatgatc
     4561 gtctctaaaa aacaagaaat aatcagtcag tcagatcaat tggaagcatg cattgctgac
     4621 tatgcaaaag cgatggcagg agacaatgca acaatggaag ctgttaaagc atattttaca
     4681 aaaaccactg atcaatccta tcaccatgtt attccttttt cttctgtcca aaaattcagc
     4741 agtgtagcgt tggcagacaa ggtgtatgtt ttaggagcgc ccgaaatggt tttgcgcgat
     4801 caattgccgg aatatgcgga agagtttgtt tctttcgctg aaaaaggcta tcgcgtgttg
     4861 gtctttggtg tgtacgatgg gattcttgaa gaaccagtat tgacagaagc agtcacacca
     4921 ttaggctata ttctgattgc taatcctatc agaaaagaag caagagccac atttgattac
     4981 ttcaaaaaac agcaagtagc catcaaagtg atttctggag ataatccgct caccgtatcc
     5041 aatgtagctg tccaagcagg gatttcacat gctgatcagt acgttgatgc atctaaatta
     5101 tcagaagaag aatatgaagc agctgtcgaa aaatatacag tttttggacg cgtcaagcca
     5161 gaacaaaaga aaatcttcgt tcaattgata aagcgtaaca atacagtagc tatgacaggt
     5221 gatggcgtga atgatattct agccatgaaa gaagcagact gcagtatagc gatggcttcc
     5281 ggaaatgaag ccgccatgca ggcctcacaa gtggtcttac ttgattctga cttttcacgc
     5341 atgccagaag ttgttgcaga aggacggcgc gttgtgaaca atattgagcg atcagccagc
     5401 ttgtttttag tgaagaatat tttctccttt ttgctctcac tgttttcagt gatctttgct
     5461 ctgacctatc ctttagaacc atcacaaatc acattgatta gtttgtttac tatcggactt
     5521 ccatctttct tgcttgctct agaagaaaac aaaaagcgaa tcagagggaa attcatcatg
     5581 aatgtgatgg aaaaagctgt gccaggtgga ctaacagata tgatcgtagt cggtgcgttg
     5641 gtgatctgcg gggtgacttt gaatctaaat aaaacagatg tttctacagc atctaccatg
     5701 cttttgattg cagtagggtt cctcgttctc tataaaatct gttcaccgct gaataaattc
     5761 agaagtcaga tcattctttt ctgtgctagc ggtaactttt tttcggtcat ctttttgcat
     5821 aagctgtttt caatcaccgg tatctcagct gtatcacttt tgctgttgag cattctattt
     5881 ttctcggcag attcgatttt taggcatttg accacattgg tagaatattt gtttgaacgt
     5941 aaaaatgaaa aagagcaaaa gccgtttagt cgcgtagcta aaaaatttct atcgatattt
     6001 aaacgagaaa attgacgaaa agtatctcaa aaaagagtaa aaaagaacaa ccactcgtgt
     6061 atctttcgaa gcaggatgag tcatccaact ttaaaacgca cgagcagttg ctctttttta
     6121 cattttcgga atggtaacgg tgaaagtcga acctgttcca agatgactgt ctacagtaat
     6181 cgtaccatta aggagttgca cgtagtcttt tacaatcgct aaccctaatc cggtcccgcc
     6241 tgaatgtcgg cttcgcgcct tgtctacgcg gtaaaaacgc tcaaagatcc gttcctgatc
     6301 ctcaatgtcg atacctatac caaagtcttg gacagaaaac gatagatcat ctgttgcctg
     6361 atagcggata ataatctcgc tgtcgctttt tgaatattgg acagcatttt caatcagatt
     6421 tttgataatt ggataaagga tatctgtttt agtagtaaat ggttcgttct ttccaatcaa
     6481 tcgaatcgtc aaccgtttct tttcgatgat tggctgataa ttttgaacga tttgctggaa
     6541 ataaggttct gtataaattg tctggatctc gtaagaattc tcgccatctt ttgacagctg
     6601 gatgatttcc cgaatcagtt tatctaaccg gatcgcatct ttttgcatga tctcaagaaa
     6661 agaagtcaga gtttgcgggt catcttttgc accatccagt aaagtctcgg taaaaccaat
     6721 cagtgaagtc acaggagttt ttaattcgtg agagacgttg ccaacgaaat ccttttgtag
     6781 cttttctaaa cgtcgcactt tcgttaagtc atacgccacc cctagaatct gtccggtacc
     6841 ttctgaatta gtaaagtagc gcaaactcaa gtctaatgtc tgccgacctt ctgtaagggt
     6901 gatctcctga tggatcagcg gatgctcagg agtcacttga tgaatcagct gaatgagttt
     6961 tggctcttga atgacttctg tgtaccgctg ttcaggttga tagcttacca cgtttaaatg
     7021 tagctgcatc tttggattaa gcagtagtaa acgagaatca gcatcatcaa tcaggaatac
     7081 gccaatcatc agttcgttca gtaacgtata taattgctct tctgtggtag tacacgcccg
     7141 atacatctta ctcatttgtt cgctcaacgc attaatcgtc tgatacaatt cttcccattg
     7201 atcgggtgtt tgcatgatta cttccgcctg attaggattt tgcaccattt tttttagtac
     7261 gggcagcacg gttttcaatg gctggttttt ttgatagatc agataataga tcattcctgc
     7321 aagaacaagg aagaaagaca gaaagaagaa aaagacccaa cggcgaaaac tctcagttct
     7381 cggtaaaaaa acgcttgtcg gttctgcgat acgtaaaatg gcttctaact ggccactttt
     7441 tttgactggt aatgcgacat ataaaagatc ctcattcaaa gtagtacttt tgcgcaaagc
     7501 gctacctagg ttcccaccat tcaatacggc tttgatttcg ggacgggagt ttcgctgttc
     7561 attcaatgtt tcattgtttg tgtcaaataa gatctttcca gtagcatcca ttaaagtaat
     7621 ccgttcgttc gactgatgga cgaattcttc caaagcagtt ttgttttgcg ctgcttggag
     7681 gttttcgaca tctaattgcc ggatcagcag ttgtgctttt tttgtcaggt agctttcttg
     7741 ctgttccacg atctgctgtt gaaaataatg ggaaatcaat tgccagccac caaaaaacaa
     7801 actaactaaa agaataagaa agaagagacg ttcataaatt tttttccatt tcatcgtttg
     7861 ggttcctgaa acttatagcc aaatcctcta atagtcacca agtactttgg gtgttttgga
     7921 tctcgctcga ttttttctcg caaatgacta acgtgtacat ctacgattcg gctttgtcct
     7981 gcgaagtcaa aattccaaat gcggtctagc aatgtgtctc ggtcgatcac acggtctttt
     8041 cgtttcatga aataaacaag taattcaaat tctttcggtg ttagttcaat cagatggtcg
     8101 tctacggtca cttgataatt ggtcaaatca gcagtgattt gtccaatcga tagatattca
     8161 ggctcagctt catcttttga ttgcttacga ggttcaattc gtctgaatat tgctttcata
     8221 cgggctaata cttcgcgagg actaaatggt ttggtcaaat agtcgtcagc acctatttct
     8281 aggccaatga tccgatccac ttggtcatct ttagccgtca gcatcaaaat cggtgtttca
     8341 tttttttctt gacgcagccg ctgagtgatc tccatgccat ccatcgttgg aagcatcaca
     8401 tctaaaatga tgaaatcaaa tgattgttct aatgctaatt ctaatccttc gcctccatct
     8461 gtggcactgg ttaccttgta gccttctttt tccaaattga acgtcaagag cgttacaata
     8521 gaaggttcat catcaacaac tagtattttt ttcattcaaa tctcctttaa ataggtttca
     8581 ttctgtttat attttagcat agaatcagta caaagggcta ttttacaaaa gaaataaatg
     8641 gtataatagg aaggcacttt gctgaataga aaggaagagc agtcaatgat tggaatcagt
     8701 gcatgtttag gcggtctggc ctgtcgctat gatggacaga aaaaagaagt accagaattg
     8761 atggaactag ttgaaactgg agaagctgtc atggtctgtc cagaagtgat cggcggatta
     8821 ccaattccta gagatcccgc tgaaatcatt ggtggaaatg gattcgatgt gtggaaagat
     8881 caagcaaaag tatggacgac aagcggcgaa gatgtaacgg aagcgtacaa aaatggcgcg
     8941 atcaaagcct atcaaaaatt gcaggaaaaa cacattgatc tagtcatcat gaaggaaaaa
     9001 agcccttcat gcggaaccca ttcgatttat gacggaagtt tttccggaat aaaaaaaaat
     9061 ggtgccggag tggctactgc ttattttatt cagcaaaaaa tgactgtgct ctctgatgaa
     9121 gaatggctga aaactagagg tgagcagaat ggaaatcgaa aagaccaatc gaatgaacgc
     9181 gctctttgaa ttttattcga ccttattgac agaaaaacaa atgaactaca tggaattata
     9241 ttatgcagat gatttttcat taggtgagat cgcggaagaa tacgaagtaa gccgacaagc
     9301 agtatatgac aatatcaagc ggacaagcaa gatcttggag gattatgaaa aaaaactcca
     9361 tcttttttct aattatattg ttcgagaaca ggtattggaa aatatgactg catatattac
     9421 agataagtac ccggaagata aaaaactact tgaatatgta cagcaaattc aagcaatcga
     9481 agaataaata aaaggaatga agaaaatatg gcatttgaaa gcttaactga acgtctccag
     9541 caggcgatgg gaaaaataag aaaaaaagga aaagtatcag aagctgacgt aaaagaaatg
     9601 atgcgggaaa tccgccttgc cttactagaa gccgatgtta acttgcaagt agtcaaagat
     9661 tttactaagc gcgtacgcga acgtgcggta ggggcagaag tgctggaaag tttatcacct
     9721 gcacaacaaa tcgtaaaaat cgttgatgaa gaattgacga tcacacttgg ttctgaaaca
     9781 gcagagttaa ataaatcacc aaaaatacca actgttatca tgatggctgg tcttcaaggg
     9841 gcaggtaaaa cgacctttac tggtaaatta gccaattatt taaagaaaaa tgaaaatgct
     9901 cgtccgctcc taatcgcagg ggacgtatat cgaccagctg cgatcgacca attgaaagta
     9961 ttaggtcaac agctagacgt tcctgtattt gatatgggaa ctgaagtcag cccggtagag
    10021 atcgtccgtc aagggatgga gttagcaaaa gaaaagaaaa acgactatgt attgatcgat
    10081 acggcaggac gtctgcatat cgatgaaaca ttgatggatg agttgaaaca aatcaaagaa
    10141 ttgacgcaac caaatgagat tttgcttgtt gtcgatgcca tgactggtca agatgccgtc
    10201 aacgtagctg atagcttcaa ccagcaatta ggtattacag gggtcgtcat cactaaactt
    10261 gatggggata ctcgaggcgg ggcagcacta tcgatccgct cagtgacagg cgcaccaatc
    10321 aaattcatcg gttctggtga aaaattgact gacttagaag tattccaccc agatcgtatg
    10381 gctagccgta tccttggtat gggggatatg ctgacattga ttgaaaaagc gcagcaagac
    10441 tacgacgaga aaaaagcgga agaacttgca cagaaaatgc gggaaaattc attcgatttc
    10501 aatgacttta ttgaacagct tgatcaagtg atgggtatgg gcccactgga agatctaatc
    10561 aaaatgatcc caggaatgaa tcaagtccct ggaatcgaaa acgtcaaggt agatccaaaa
    10621 gatgttgaac gcaaaaaagc gatggtttat tctatgacgc cagcagaaag agcaaatcct
    10681 gatctgttga acccaagccg tcgtcgccga attgctgccg gttctggaaa cagtgttgtt
    10741 gaagtgaatc gaatgatcaa acaattcaag gaatcaagaa aaatgatgca gcaaatgtcc
    10801 aaaggggata tgaacatccc aggtatggac caaatgtttg gttcaggcgt caaaggaaaa
    10861 ctaggcaaaa tggcgatgaa tcgaatgatc aagaaaaata agaagaaaaa gaaaaaacgg
    10921 aaataagatg aaaaaaacag cttcggtaca gactgaaaat gtctactgcc ggagcttttc
    10981 tatcagcaat ttttattctt tactaataca attactctaa acgaagaggt ggcacagatg
    11041 agatgtccgt ggagtgaaca aacagaaagt atgcggcaat atcatgatac tgtctggggt
    11101 gttcctgaat atgatgacca aaagctgttc cgaaaattga tgcttgatat taatcaagcc
    11161 ggattgagct ggcagacgat tttaaataaa agtgaagcat tcgatgaagc atatgatcga
    11221 ttcgatatca aaaaagtcgc aaattatgga gaaacaaaaa tcagtgaact aatggaaaac
    11281 aaaggaatta ttcgtaaccg aagaaaaatc gaagcagccg tccaaaacgc caaagcagtc
    11341 ttgaaaattc aagaagaaat cggcagtttt tcagagtatc tttggtcgtt ttctaatgga
    11401 aaaatcgttg attcttctta tcattcacaa gaagaagtcc caacaacaag tcctctttca
    11461 gatgaattag caaaagatct aaagaaaaga ggatttaaat ttatcggaag cacaacaatc
    11521 tatgcttttt tagaagctgt cgggatcatc aatgatcacc ttgacagttg tttccgcaaa
    11581 gaagaggtcg tacgtatcgg cagaaagggc gaataaagca tgcgaaaaat catattttat
    11641 ggtgctataa gtttagatgg ctatttggca acgaaaacgg atgatcttca atggcttttt
    11701 gacactccaa caggtaagca aactacttat gaagcttttt atgaaacaat cgatacgacc
    11761 atcatgggca gaaaaactta tcaggaagca aaaaaatatt tggagaccga agcaatttat
    11821 ccagagaaaa agaatttcgt tttttccacc cacgcacaat tacaattgcc agatgctgag
    11881 gtcgtaaacg aagatcctgt tgcttttcta aaaaatctac aaatgacaga aggaaaagat
    11941 atctgggttg tgggtggcgg tcgattgatc aaaccgctat tggaacatca aatgattgat
    12001 gaatggtata tccagattac ccctgtatta ttaggggacg ggattccttt attccaaaaa
    12061 ggaacatatg aagcgcgttt tgaaatgcta gacacgacac gattcggtga atttatcgaa
    12121 ttgcattatg tacgaaagta gaagtaaata aaataaaaaa ggcaactata tgcagaaatg
    12181 tcctgtacat agttgccttt tttattaccg ttatgctttt tactctgtta atttgttaga
    12241 aaaatctaga tacgcatcta aaatcgtttg gaagaaactt aatgttcctt caacgatatt
    12301 accattttca tcaattagat tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt
    12361 tggcacattc aaaaagacaa gtgattggcg tagatggtgg tttgcgccaa agccgccgat
    12421 cccacctggt gatgcagtaa cgactaaacc tggtttatga tcccaaacac tgtgtccata
    12481 aggtcttgag cctacatcta aagcattttt caatactgct ggcacagaac ggttgtattc
    12541 aggagtaacg aaaacgacac cgtctagtcc cctcacttcc tcacgaaaac gagtccattc
    12601 agcaggagcc tgttctggag tttcgagatc ctcattgtaa aatggcagat catcgatttt
    12661 tacaaataca ctttcgtacc cttcaggaag caatttcgca aaagcttctg ctactttttt
    12721 gttgtaagaa tcttttctta aaataccaat aaaaaatcca attttttttg tcatacgatt
    12781 tttcctcctt caaactatct ataataatct tac
//
LOCUS       DADXTG010000072        12752 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.073, whole genome shotgun sequence.
ACCESSION   DADXTG010000072 DADXTG010000000
VERSION     DADXTG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.073"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            229..798
                     /locus_tag="IWI57_001750"
     CDS             229..798
                     /locus_tag="IWI57_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBC2449892.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            785..1645
                     /locus_tag="IWI57_001751"
     CDS             785..1645
                     /locus_tag="IWI57_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBC2449893.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1835..2539
                     /locus_tag="IWI57_001752"
     CDS             1835..2539
                     /locus_tag="IWI57_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2449894.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2544..4379
                     /gene="walK"
                     /locus_tag="IWI57_001753"
     CDS             2544..4379
                     /gene="walK"
                     /locus_tag="IWI57_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBC2449895.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4376..5692
                     /locus_tag="IWI57_001754"
     CDS             4376..5692
                     /locus_tag="IWI57_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449896.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5693..6562
                     /locus_tag="IWI57_001755"
     CDS             5693..6562
                     /locus_tag="IWI57_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBC2449897.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6617..7426
                     /locus_tag="IWI57_001756"
     CDS             6617..7426
                     /locus_tag="IWI57_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBC2449898.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7529..8158)
                     /locus_tag="IWI57_001757"
     CDS             complement(7529..8158)
                     /locus_tag="IWI57_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBC2449899.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8328..9620
                     /locus_tag="IWI57_001758"
     CDS             8328..9620
                     /locus_tag="IWI57_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2449900.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9696..10358)
                     /locus_tag="IWI57_001759"
     CDS             complement(9696..10358)
                     /locus_tag="IWI57_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBC2449901.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10583..10867
                     /gene="groES"
                     /locus_tag="IWI57_001760"
     CDS             10583..10867
                     /gene="groES"
                     /locus_tag="IWI57_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBC2449902.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10918..12543
                     /gene="groL"
                     /locus_tag="IWI57_001761"
     CDS             10918..12543
                     /gene="groL"
                     /locus_tag="IWI57_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBC2449903.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4232 a         2190 c         2773 g         3557 t
ORIGIN      
        1 gttcttgcaa gagaacaaac cctaacactt ttttcatttt ttactccttt cccgccgtct
       61 ccccggcggg tctcctgttc ttaacgataa aagacaagaa ggcttagcct tcttgttttt
      121 tgttttcatg gaatcccgct aatcaaatca cctagttaaa gcaactagat tgaatgttta
      181 tcataagtct gatatcatgt ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg
      241 cgtcagcaat taatggagtg ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa
      301 gaactccctg acttagaact ttatatggac caagtcagaa cgttagttga tcgttatctt
      361 tcacctgtga tacaaggaga aaagcatcca ttgcttacct cttctatggt aaacaattac
      421 gttaaactag gtttgatacc agcaccagtt aaaaaaagat ataacaaaga acacgtagca
      481 tttttacttg caatcactac gttgaaacaa gtattgacta ttcctgaaat caaagaaggg
      541 atattgttcc aaggaaaaac agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa
      601 caagaagcag ctgtgtggat ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa
      661 ttcgaccagg caacccctgt agaatatatt gctgtaagag cggcaacttt atcgtttgca
      721 atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg aatatttaaa agaggagaaa
      781 acaaatgaaa aacaataaaa tcgcaatttt agtagattca ggtacggatg tgccagaaga
      841 attgataaaa aaatacgaga tgtatatgat cccattgaaa attatttata aagatcaggt
      901 atatacagat aaaattgaca ttacctctga agagatctat caacgtttgc cagaggaaat
      961 ccctggtaca tctttgccgg atggtgagtc gatcacagaa atctttgagc aaatcaaaaa
     1021 agacggttat gaaaaggtac ttgcggtaac aatttccagt ggattgagcg ggacatataa
     1081 tgtggttcgt ttaattgcac aacagcaaga agaattggaa gcatatgtac tagatacgaa
     1141 aaatattggt attggtgcag gtttttctgc gatccaagca gcaaaatggc tggaagaagg
     1201 agtagaatgg gatcaactga tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt
     1261 ctttaatgtt gcgacattgg aatatcttca aaaaggtgga cggatcggtt tagttgcttc
     1321 tattgtagga accgctttaa agcttaaccc aatcatctca tgtaatgaag aagggattta
     1381 ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt
     1441 caaagaaaaa atcggtaatg caaaggtctt caacttagct gttgcccatg gggatgcgaa
     1501 agaagaagca attgaaatgg aagcgagact taaaagagag ttcccacaag caaaccaaat
     1561 ctactttggc cagatttctc cggcattagt agtccacact ggtccgggat tattgggtgt
     1621 cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg
     1681 ttttcctttt ttattgaagt tagaacaaaa aataataaag tattttgcac tttcatttta
     1741 attttctctt ttgttttata ggcaaagatg aaacaaaagc agaaatacgg tataatgaga
     1801 aggtatgaca ataatatata agaggtggct ctttatgaaa aaagttttag ttgtcgatga
     1861 tgagaagccg atttcggata tcgtcaaatt caatttagcc aaagaaggct atgatgttta
     1921 tactgcatat gatggggaag aagccttgga aaaagttgct gaggtagaac ctgatttgat
     1981 tttacttgat ttgatgctac ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa
     2041 aacttatgat atgccgatca ttatggtaac agccaaagat tctgaaattg ataaagtcct
     2101 aggcttagag ttaggcgccg atgattacgt gaccaagcca ttttccaacc gagaattagt
     2161 tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc
     2221 agcacctgcg gaattgacga ttggtgattt gacgatccat ccagaagctt acatggtgac
     2281 gaaacgagga gaaacaatcg agttaacaca tcgtgaattc gaactactct tttacttggc
     2341 gaagcacctt ggacaagtga tgacaagaga acatttatta caaacggttt ggggttatga
     2401 ttattttgga gatgtacgaa cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga
     2461 agataatcca agccatccaa attatttagt gacacgtcgt ggtgtcggtt actatttgcg
     2521 taacccagaa caggagtaat tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt
     2581 ttaagatcgc tattaccttt attctgattt tattgatctc gatcgaaatt attggtgcct
     2641 actttatacg tggtttagaa cgttccacga tcaatacatt tattaaagat atgaatcaaa
     2701 cggtggaatc attagcaaca acaatcagcc cggaattgaa tcgaaaagat aatgcagatg
     2761 atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa cagtgcgact agcgatatca
     2821 tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac
     2881 aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa tgatttcacg actaaacgtt
     2941 atgttgcttt agataacgac gataaacggg tgaatatcaa tgttcaaccg atcctgtccc
     3001 cgaccggcga tgccgttatc ggggctcttt acgtcaaaag taatatcgaa caaaaatatt
     3061 cggaaatcaa taacacagca gtcattttct ttactgcctc actaattgcg gcatttattt
     3121 caatgatcgt atctgtttta gttgctcgtt cgattacaca gccgatcggt gaaatgagag
     3181 aacaagccat tcgaatcgct agaggagact acagtcggaa agtcaaagtg catggacaag
     3241 atgaacttgg acagctggct gataccttca atcagttagc tgaacgtatt gaagaagctc
     3301 aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt gctatcccat atgacagacg
     3361 gcgtcattgc gacagatcgc aggggtaaag tcattacgat caatgatatg gctgtttcct
     3421 tgctagatat caaaaacgag gaagcaatcg gacaatcgat tttgacactt ttagatatcg
     3481 aagaagaata tacgttgcgg aaattattag aagatccaaa cgaaatggta cttgaccgtt
     3541 caaccagtac attggaaagt gatcagatga tcttgcgagt agacttttct atgatccgtc
     3601 gcgaatcagg ttttatcagc ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa
     3661 aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt
     3721 tgacaagtat gcgtagttat atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag
     3781 tagcaccaaa ttttttgaag gtaacgctag atgaaacaga ccgaatgatt cgaatgatca
     3841 atgacctctt aaatctatct cgcatggata caggtaatac ccagcttcaa ttagaatatg
     3901 tcaatttcaa cgagttagtg aattttgttc ttgaccggtt tgatatgatg gtgggaaatc
     3961 aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag
     4021 atacagataa aatcatccaa gtagtcgata atatcatgaa taacgcgatc aagtattcac
     4081 cagatggggg aacgattact tgtcgcttat tagaaacgca taataacgtg gttttaagta
     4141 tcaccgatca aggattgggg attcctaaaa aagatctgaa ccgagtgttc gagcggttct
     4201 atcgagtaga taaagcacgt gcaagagcac aaggtgggac aggacttgga ttagctattt
     4261 ctagagaagt gattaaggcg catcgaggcg ctatttgggc agagagcaag gaaggaaaag
     4321 ggtcgacttt ttatatctcg ctgccatatg aaccttatga ggaggaatgg tgggaatgag
     4381 aatctctgaa aaagtcgttc gtataggact cattttgatg gttgcgttaa gcatatactt
     4441 ctcttatgca atctggctga gcccagcagg aaaaagttca atcaacgtag atgagacaaa
     4501 cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca
     4561 tgttacatgg caccattcgg gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg
     4621 attgcaattg atcatcgata aagcaagatt tggaaagctt tctgaagtag tttcaggaga
     4681 caaatcacag tttgaacagg tgaaaaatct gaactctggt tttgagttgg cctataatgc
     4741 gccttttttg ctaagagaat acaaggaagc atttgattta gatttagatg tgtcgaactt
     4801 ggcaaaagaa agcgaaaata tgtattttac tagaatacaa ttcgatcaag gaaataaaaa
     4861 aatccgattt ttgaattatt cgaaccatct cgtatatgaa gcaaatattt cgttgaattg
     4921 gacagatatc aataaagaat taacagcaaa agacacaaaa tggacagaaa tgacaacaga
     4981 accatcgacg atcgacacgc aatatgctac gaaacattcg atcaaactga aaaagtatag
     5041 ttatatcctg tcacagcaac cctatacact ttttagaaat gcttttttcc agaaaccaga
     5101 tagcgtgaaa aataatgatg aaagcaatga attgattttt tacggtggga atgaaacaat
     5161 gtctatccat tcggaaacgc aagtagttga ttttcgtgga gagctgccag agaaaaaaga
     5221 aaatgctagt gtattttctg aaagtttccc ctatattagc aaattaggca gttctttagg
     5281 gaacctcaga tattttgacc gcagtgacaa tacgatcgat tatcggatat tcgtggaagg
     5341 cttcccggtt tttgggacag acagcagagg acagattggt gtggaagttg gcggtgaaac
     5401 gaatggttct gtgcaagtga atatccaaac cagtttgaat acgattcaag tgccgatacc
     5461 ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc atccagcagt taaaagctaa
     5521 gggagcagat agcaacaaga ttggttcagt gatcgttggc tatacatggc aaaatgtcaa
     5581 agaaacgaat cgtgtcgttg atctcttacc tgaatggtat gtgaaatata aagataactg
     5641 gtattcagct aacgaccttc tggaaaaatt acctggattg gaggcgaatt aattggattt
     5701 caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag
     5761 cagttatcat gacgcggtat accaagaaaa caatgtcatt cgctctaatc agactgaatc
     5821 aatccaacaa cgtctggcta gcgacgatat cacttacacg gggaagattt cgtctgagaa
     5881 caagcaaggc tattatctta gcgcggagca gaccaatctt agcacttcat tagcgaatta
     5941 tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg tttttagacg gtgatgtttt
     6001 gacgaaatct ttatccagtt cagatgctga aaagaacgtg atcgatccag ataaaattag
     6061 tagttccttg gaagattttt tatctaacaa agacaatatt ttattcggta atgattatgt
     6121 gtacacgaaa aacttttctc atttagaaga agaaccacta gaaatccaag caagccaaga
     6181 gtatgaaggc atcccattca atgatgatac agcaaaaatc gttctttctt tagaaaaaaa
     6241 cgataatgat tatagcatca cgaaatacat gcagacgcat ttatctgata tcgagcagct
     6301 gcgcgaaaag acggaactgt atacggaaga ggaagcaatc aatacattgt acatcaataa
     6361 caaaatacca agaggatcaa aaattttatg gcgtcagcta gcttattcct gtattctgaa
     6421 agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta gcgatcgaaa caagcgataa
     6481 agtggtccaa attgagagtg tcaacgcttt cagcaatacg atcgtgacaa acaatactgt
     6541 accaaaagtg gaagatcaat aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa
     6601 gggaagtagg agtcaaatgg ctgaagaaaa accagctttt aaaatcagta tcctagcaag
     6661 tggaagttcg ggtaattcac tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc
     6721 aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa attgatcgga caccggacca
     6781 attagatgga atacttgtga cgcatgaaca tcgagatcat atacatggcg tgggtgtctt
     6841 ggctcgtaag tatcatttgg atgtttttgc caatcagcag acttgggaag cgatggaacc
     6901 actgattgga aatgtaccag tggaacaaaa acatctattt gagatgggga aagtcagaac
     6961 attcggcgat atcgatattg agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt
     7021 ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg acggacaccg gatattgcag
     7081 tgattatatg cgaggtgtca ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga
     7141 tcttgagatg ttacgcatgg gaccttatcc gtggaattta aaacagcgta ttttaggaga
     7201 tagaggacat ctttccaacg aagacggtgc acttgtcatg actgatgtga tcggtaatcg
     7261 gaccaaacga atctatcttg gccatcttag caaggaaaac aatatgaaag aacttgctca
     7321 tttaacaatg gaaaatgttt tgaaagaaaa agattttggc gttggacatg attttgaaat
     7381 ctatgataca gatccagatg aagcaactga gctattttcg atttaaacag ttcaatcaat
     7441 aaaaatataa aaagcagaag ataaaatcaa tcagggttgg ttttatcttc tatttttatt
     7501 tttttagcat attaaaaaaa taaccgtatt atcgactgac tacactgatg tgctgctttt
     7561 catgtttctc atccagtatc tcatctacta atgcaatcgc gtaatctgcc atgctgatcg
     7621 tactgtcgcc agcagcgttt ctttgtaaat aatcatcact taacaaataa gtacctgtac
     7681 gtttgccatg tggatcaaat aaagaagctg gactgatata agtccaatgt agatttttag
     7741 attgtttcaa ttttaacaat ccttcgtaca tattgaaagc tgttggatag aagggtgcat
     7801 ttttaggtac ttggtcaatc atacgagtcg tcttttctgt atctaagaat aacgaagagg
     7861 cacccccaac gacaattaag cgtgtatcgg ttccttcaag catctcaatc aaatgggaca
     7921 aagaagtttg atgcatttct tcttttcctt tgggagcgtt gaaagcatca acgacaacat
     7981 caaaagatgc aagatcagta gttgtcaact caaataaatc cttttgtata aacggaacat
     8041 ctgatggcaa tttttctgga tgacgcacga ttgccgtcac gtctaaatct ctggacaatg
     8101 cttcgtctaa aataaatgaa cctgcatggc cagtagctcc aatgagtgca attttcataa
     8161 aaaacctcct tttttattcc tgtccattgt aagcagttgc tgaatattcg tcaaagaatc
     8221 cgatttttga aaagctcagt gcgcattcct ttcatggttc gtgatcattg ggtatagtta
     8281 aattaagaaa actgattgat aaaaatagaa gttgaaggag ataagaaatg aaagagtggt
     8341 tagatcgtgc ctttttacca atgataacat ctgttgtgaa tcttagaccc ttaactgctt
     8401 tgcgtaatgg aatgacttac acgatcccaa taattattgt tggctcgctg tttttcttat
     8461 ttgctcattt tcctgtggat tacatcgctc agtggataga caaaacagga ttttctatgt
     8521 attttacaca agtataccag gctacatttg atgtgatagc cttttggatc gtttttgcca
     8581 ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt accagtcggc ataacagcct
     8641 tgtctgctta tctactgctt attcagccac taaatactca gatagatact gtctggacag
     8701 gtgctgaagg gatactagga gcagttacaa ctgggctcat catcggatgg ggctatgcac
     8761 gtttagtaag acttgcgaaa gggtggaagc aaagagagaa gatacctgaa aatgtttggc
     8821 agtcttttac ttcattgatt ccaatcattg ttatagtcac ttttacgctt atcggttcag
     8881 cggtttttca acagttgttc agaatgactg ttgttcaagg agtatggaca tatgtccagc
     8941 ttcccattca atcgttcata gacacgttgt ttggtgtgct tttattaggt ttttttgttc
     9001 cgtttttatg gttatttggt cttcatggat ctgcgatggt caatgggttg gttagtcccg
     9061 tcttgcaagc gaattcatta gcgaatgcag aaatcttagc ctcaggacaa gagttgacgg
     9121 tggcaaatgg cggacatatc gttactcagc aatttctcga tcagttcatg acggttacgg
     9181 gtgctggatt gacgttaggg gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat
     9241 acagagaatt aggaaatctt gctcttttac cagccttttt caacatcaat gaatcaatta
     9301 ttttttccac accgatcgtg atgaacccga tgatggctgt tccttttatt ttcgcaccgg
     9361 tcgtttcagg attgattacc tattccgctt tatattttgg attcgttcct ttatttacag
     9421 cagtccaagt cccttggaca acaccgccga tcctctcggg tttcttagta ggcggagtgc
     9481 ctgcagcgat tttacaaggt atcgtgttgt caattagttt tttcatttat ttcccatttt
     9541 tgaaaaaaat agattctgca aactgcaaaa aggaaagaca gacaaaaaat gatggagcag
     9601 cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc gctttagatt gggaaaatca
     9661 ccaatctagg atgacaggag tatttatagt catgtttata ctagcccttt ttggacagct
     9721 aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc
     9781 cgaccagttg ctttgtactc taggctgaag aagaaaccta ggaaaaagta gattagcaga
     9841 tgaccatcat tatgagcaaa cgcaaacagc aaagagctga tcactgctcc gacccaaaaa
     9901 tttgaatatc gactgatgat ccccagtata ctgcggcgga atacgaactc ttccatgatg
     9961 ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt ccatgatcat ctggatgata
    10021 ttttgtgtat tctcggaagg ggttgctttg ccaaacaata cagattctaa catgacagcg
    10081 atgctttgca gaatcaatgc gacaaagatt ccaaccaatc cataaataac aatctttgga
    10141 gcagaagctt tttttaccgc tgattcgatc gctaagggtt cacgttgctt aaaataaaaa
    10201 atagctagga caacggcgcc tagtatatac atgactgcag ttgctgtgat caattcagac
    10261 gtagatttta caagtcctat cgaagctagg atcaagggtg agataaaaat tagaccatag
    10321 caaaaaatgc tgaggaagct atattttttt aaagacatgt aattcctcct tttaagggat
    10381 aacaagcatg caaggcaagc ttatatcgat tgatttcaat tatatcataa tttcaataag
    10441 cagctgtcag aataaaattt cttttctttg atttaaaagc ttgcaaaaaa gagggagaat
    10501 gagtattatt aaaaatgtag gttagcactc gaaggttaag agtgctaaca gattaaaaaa
    10561 agagttatcg gagggattta tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt
    10621 cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa
    10681 accccaaact gggacagttg ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa
    10741 agttcctgcc gctgtaaaag caggcgacca agttatgttc gaaaaatacg ccggtacaga
    10801 agtaaaatac gaaggaaaag aatatctgat cgtcgcagga aaagatatca tggcgattgt
    10861 tgagtaaatt tggaagataa aaaaataatc aaaaataatc aatgaggtga atcaatcatg
    10921 gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta tgttacgcgg ggtagataaa
    10981 ttagcagata cagtgaaagt aacattagga cctaaaggcc gaaatgtcgt actagaaaaa
    11041 tcttacggct ctccattgat cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta
    11101 gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat
    11161 gatattgcag gagacggtac aacgacagca actgttctga cacaagcaat cgttcgcgaa
    11221 ggtttaaaaa acgtaactgc aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg
    11281 gcaacaaaag cagcagtcga agaattgcat aatatctcaa ctgttgttga ttcaaaagaa
    11341 gccattgcac aagtagcagc tgtttcttcg ggttctgata aagttggaca tttgattgct
    11401 gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc
    11461 gaaacagaac tagatgttgt agaaggtatg cagtttgatc gtggttactt atcacaatat
    11521 atggtaacag ataacgacaa aatggaagct gttctagaaa acccatatat cttgatcaca
    11581 gacaaaaaaa tctctaacat ccaagatatt ctgccattgt tagaacaaat cttgcaacaa
    11641 tcacgtccat tgttgattat tgctgacgat gtagatggtg aagcattgcc aactcttgta
    11701 ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa aagctcctgg tttcggcgat
    11761 cgtcgtaaag caatgcttga agatatcgct attttgacag gtggtacagt aattactgac
    11821 gatctaggtt tagaattgaa agatgtaaca atcgaaaact taggaaatgc ttctaaagtt
    11881 gttgtagata aagacaatac aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa
    11941 gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa
    12001 aaattacaag aacgcttagc taaattagca ggtggtgtag ctgttgttaa agttggtgca
    12061 gctactgaaa cagaattgaa agaattaaaa ttacgcattg aagatgcatt gaatgctaca
    12121 cgtgccgcag tagaagaagg aatggtctca ggtggtggta cagctctagt gaacgttatc
    12181 agcaaagttt ctgctgtaga agcagaagga gacgtggcaa caggtatcaa gatcgttgtt
    12241 cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg
    12301 attgttgata aattgaagaa cgtggaacta ggcactggtt tcaatgctgc tactggagaa
    12361 tgggtaaaca tggttgaagc aggtatcgta gacccaacta aagtaacacg ttctgccttg
    12421 caaaatgccg cttccgtttc tgccttgtta ttaacgacag aagcagtcgt tgcagataaa
    12481 ccagaaccag cagcgccagc agctcctgca atggatccat caatgatggg cggtatgatg
    12541 taagaaaaag caacttgctt atctacaact gatcattagt gaaagacaga taaaaagagg
    12601 ctggaacaga agcgtttaac tccaagaaat aagaagaaat tcacggaaat tgcttttcaa
    12661 atttttgtga atttcagctt atttccgaag gagttgcttc tgcttccgct gtttatacgt
    12721 gtttagagcg tgagacaaaa gtgttcttta ct
//
LOCUS       DADXTG010000073        12470 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.074, whole genome shotgun sequence.
ACCESSION   DADXTG010000073 DADXTG010000000
VERSION     DADXTG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.074"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWI57_001762"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWI57_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBC2449904.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWI57_001763"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWI57_001763"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBC2449905.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWI57_001764"
     CDS             complement(2169..2906)
                     /locus_tag="IWI57_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBC2449906.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWI57_001765"
     CDS             complement(2997..3524)
                     /locus_tag="IWI57_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449907.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWI57_001766"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWI57_001766"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBC2449908.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWI57_001767"
     CDS             complement(4401..4886)
                     /locus_tag="IWI57_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBC2449909.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWI57_001768"
     CDS             complement(5039..5236)
                     /locus_tag="IWI57_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449910.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWI57_001769"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWI57_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBC2449911.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWI57_001770"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWI57_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBC2449912.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWI57_001771"
     CDS             9211..9423
                     /locus_tag="IWI57_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBC2449913.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWI57_001772"
     CDS             9425..11104
                     /locus_tag="IWI57_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBC2449914.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IWI57_001773"
     CDS             complement(11358..11558)
                     /locus_tag="IWI57_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2449915.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IWI57_001774"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IWI57_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IWI57_001775"
     tRNA            complement(12249..12322)
                     /locus_tag="IWI57_001775"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
BASE COUNT         3641 a         2678 c         2168 g         3983 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg
//
LOCUS       DADXTG010000074        12462 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.075, whole genome shotgun sequence.
ACCESSION   DADXTG010000074 DADXTG010000000
VERSION     DADXTG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.075"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWI57_001776"
     CDS             140..460
                     /locus_tag="IWI57_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449916.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWI57_001777"
     CDS             912..1112
                     /locus_tag="IWI57_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2449917.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IWI57_001778"
     CDS             1259..4048
                     /locus_tag="IWI57_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBC2449918.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IWI57_001779"
     CDS             4095..4622
                     /locus_tag="IWI57_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBC2449919.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IWI57_001780"
     CDS             4643..5833
                     /locus_tag="IWI57_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBC2449920.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IWI57_001781"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IWI57_001781"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBC2449921.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IWI57_001782"
     CDS             8224..8898
                     /locus_tag="IWI57_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBC2449922.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IWI57_001783"
     CDS             8895..9236
                     /locus_tag="IWI57_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449923.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IWI57_001784"
     CDS             9266..9625
                     /locus_tag="IWI57_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBC2449924.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IWI57_001785"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IWI57_001785"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBC2449925.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4174 a         2157 c         2682 g         3449 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa ta
//
LOCUS       DADXTG010000075        12391 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.076, whole genome shotgun sequence.
ACCESSION   DADXTG010000075 DADXTG010000000
VERSION     DADXTG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.076"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..382)
                     /locus_tag="IWI57_001786"
     CDS             complement(<1..382)
                     /locus_tag="IWI57_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBC2449926.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTF"
     gene            complement(372..2546)
                     /locus_tag="IWI57_001787"
     CDS             complement(372..2546)
                     /locus_tag="IWI57_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HBC2449927.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2562..4904)
                     /locus_tag="IWI57_001788"
     CDS             complement(2562..4904)
                     /locus_tag="IWI57_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449928.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4926..7469)
                     /locus_tag="IWI57_001789"
     CDS             complement(4926..7469)
                     /locus_tag="IWI57_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2449929.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7479..7877)
                     /locus_tag="IWI57_001790"
     CDS             complement(7479..7877)
                     /locus_tag="IWI57_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2449930.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7958..8242)
                     /locus_tag="IWI57_001791"
     CDS             complement(7958..8242)
                     /locus_tag="IWI57_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449931.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8266..9264)
                     /locus_tag="IWI57_001792"
     CDS             complement(8266..9264)
                     /locus_tag="IWI57_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2449932.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9257..9604)
                     /locus_tag="IWI57_001793"
     CDS             complement(9257..9604)
                     /locus_tag="IWI57_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449933.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9620..10270)
                     /locus_tag="IWI57_001794"
     CDS             complement(9620..10270)
                     /locus_tag="IWI57_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449934.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10281..11453)
                     /locus_tag="IWI57_001795"
     CDS             complement(10281..11453)
                     /locus_tag="IWI57_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBC2449935.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11472..12344)
                     /locus_tag="IWI57_001796"
     CDS             complement(11472..12344)
                     /locus_tag="IWI57_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2449936.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
BASE COUNT         3394 a         2700 c         1991 g         4306 t
ORIGIN      
        1 agaatgtttt tctctcaatt tttttgacgt ttctatcatg aatcacgaga aaacttggga
       61 cacttttaac atggtaacgt tgtaaaaaag tttgaagttc ttcttgtctt ggttcttttc
      121 ttgtttcaat tcgatataca ttcctagaga attttttgtc gtatctttcc aagaaagatt
      181 caacttcttg gcatttttta caatcatttc ttgctagata cacaaaaaat gattgattcg
      241 ttttttgaag ttctagtact tcttctaaat tactattgat tagttttcca tttgattctt
      301 cgttagtttt tatcagttta aaacaaccaa ataagaggaa actaacaata agtatcctaa
      361 caataacttt cttatttcgc atattgtttc tccaaatttg gtttccgaat atagacgact
      421 cgcggtggtt cctggacgtc ttcagctgtt cgatacacca caaattcgcc tgttctcaat
      481 gctcgaatgc gattcggatt gaatttaaag cggtctacgt ctcgtttcgt gcctctttct
      541 cctgtccaat caacctttcc catatcagaa taacctgctt cttgtgtcgt catttccgtt
      601 aattcaatgt cttcttctgt ccccattaaa tctgcccaat aatcaatttc tgtcgggtca
      661 tttgtcttcc caataaagaa cgtattcaca ttcccaatca actgttttcg aaggacagga
      721 tcaattctat caatgtccgc aagcgtttga ggggcatacg ccgcatgcac cccagcgcct
      781 cgtcccttat tgactaagtc tataaggtta tcatttagat aagaagctgg ttcgtcaaat
      841 attactagga ctttcgagcg attggctgtt tctcctgctt gtttatacac ttctgacgca
      901 aagaaattaa tatctcccac aagcatttga gccaatgtcc gaataaactc actataaatc
      961 aatccgttta acgagatata aacaatcttt ccttctttaa caacttcttg aatatctaat
     1021 tctctttttc ctccagaagt atcaaagagt tcaccaagct cactatccaa taacatattg
     1081 atattggtct gtaaattctc agaagtcgca tacaaataaa acgggttctt cttatgttcg
     1141 taacgaacaa acaaccgttt aaacaacgtt tgctcgattt ctgtcatgag agaaatgttt
     1201 tcttttaata atagataata cgtttctaag tcaacggttt tacgatcatg ttcaacgatt
     1261 cgctcttcac ttaactgttc cacatcggca aaatcacgtt ctgttttgtc catcgcttca
     1321 ttctttttcg tttttttctt cgtggttttc ttcttttcta cgaccacttt ttctttaaat
     1381 aaatctggat ttttcaactg aatacgatcc gcaaacaaat gtaagacgtt tcgtggcaat
     1441 aaataatgtt gtaaccaaac tagatttctt tcaattttat ggctcttttg gaactcgtct
     1501 aaaagctgaa tcgttacctg taataacgct ttggctgctg tcgaataata cacgctttct
     1561 gtttctgcta gattggttaa tttatctcga atggcaatat cattgccatt ttttacagga
     1621 ttataacgca aatcgccacc gtccacaaag acttcaatcg ggcgattgta tgcctctgcc
     1681 acttctttta acgcttgtaa agtctctcct gctcctttcc catcgactaa gataacgggg
     1741 atacgaaact tacacgcatg ttctgctagt aattggatca ccgtcgtttt tcctgaacct
     1801 gtagtcgctg gaataatcac atgttggttc aattcactaa agttcatcac ataagggagc
     1861 ccatactcat ctgttcctaa tagaatcact ttggctaatg aagccagttg tttattccgc
     1921 aaagcaacgt cttttatgac aattgctacc tggtacgctt gctgatactc tgctgtatat
     1981 ttttgggact cctgaaattt attcttccga cgttccttgt attctttact atttagcaaa
     2041 gctgtttgcc gttctttctt tgaaacaact tttctttttt caataaaaga cgcatacgca
     2101 atcaaacaac ttcctgcacc tgtcccacct aaaaggaggg caaaaatcgt ttgtccatta
     2161 aactgcaacg catgagaaaa caagtttaat ggaaataata agtaacacga caaataccct
     2221 ctaggaagaa caaaaataaa acaaaataga tttaatagcg taaaacctaa cgcaaaaata
     2281 aaaaaaagag agcttcttcg ttttaagaag ctctcatact caaagtcata aatatgtgca
     2341 cgtgtctctt ttttatctat cataaagaca atcgctaaag gaatactgcc tacaataaaa
     2401 aatggatata gacaaacacc taataaagtg acgataaaat aaagttgttg aaaccgactt
     2461 tttggcgttt ctttttcttg ttttcttttg cgatcgacag aaaacatttg ctcaaaatgt
     2521 gtcggtcgtt ttggtgcatt catcatacta tcgatttcct cctatcgttt tcgttgtttc
     2581 atttctcttc gataaatcgg ttgtttttct cgttttcgtt tcatttttgc ttgagttgct
     2641 cgaaactcac tccgtgtctt cggtgcttgt tttggtgatt ttgtttgttt catggttgag
     2701 tatgctttag atacggttga aggcgctttg gtttgtgctg ttactcgatt gactgctgac
     2761 atatgttccc gaaccccctc tggacgtcga acagatttcg gtgtggtaga acctgttgct
     2821 cgtggtacat tcccttttct tagtggtttt tgtaccgcat gtttttgtcc tgcaactgtc
     2881 cgggttttca tcgaacttgt ttttgttacg ggagtaagtg cctccttccg acgacgactt
     2941 gtttgtccct gacgacgttt tcgatcgttc aattctgttc taggcgtatg cttctggcca
     3001 taagacttgt gtttttgctt tggattcgct aatgacacca ccattgcaga accctgattg
     3061 cgatacggac tacgagacgc accttttcta cttctttctc ttagactagg attgccttgc
     3121 aattctcgtt gtaataaatc ggctgttttt cttgcctgtt tctccattgc tgttcgttgc
     3181 atacgttctt gactttgctc ttctgcttgt ttcgcttgat catgcaagtc tcttgctttt
     3241 tgtgccatac gttccatacg atttggtgct ttatgacgag gttgttgtgc ggattcacgc
     3301 cgaaaagcac ttcgtgtggc ttgtttcgcc gtttgtggca tacgaacgtc tgagccaccc
     3361 ccacgttttc gttgacgttc ttgtctcttc cgtgcgtttt ctttttcttt ttgtttctgt
     3421 gcttttctcg cttctttgcg ttctttcaac cgttgtttgg cttcccttct caattttgct
     3481 tctgccccaa atggatttcc cgcaaaagct aacgctcctc gaacacccat tcttccagta
     3541 aatagactgc ctaaaaagcc tcggaacaaa taaattccca acggcgcaaa caaataaaca
     3601 atgatcaaga gaattttttc ccatggatcg tcttgtgtac tacttaattg gaacccgtaa
     3661 ctaagaaaag aagaaaaaag gatacatgca aaacctgtca ttccttttaa aaacgtcaaa
     3721 ataccaaaaa ctttggcata gttaccaaac acattcattg tgacaagaaa caatcctaaa
     3781 aaaagtaaga acggcaacat cggtactaac aatagttgca tagttccaat aatcaatcga
     3841 aacatactta agacaaaata aacaacggtt tgaatcagat tcacgacaaa ataaaacaag
     3901 gcaaatgctg tatttttcca actattgtaa tattgtatgt tcttgttttt cagtgtatct
     3961 gcttcatact ctgtgacttt gtcatgaatt ttctccccta tttttgtacc taaatcattg
     4021 tccaataaaa gattgattcg atcatattct tttccagcat atttgacttt cttctcacga
     4081 attgtgtcta cattggttgt gccataattt aacaacagat atggttcata aacattacta
     4141 taaaagaccc gagccgccat caatttgcct gccgtttcca cttttttatc cgctgttacc
     4201 tcttctgttt cttttgtttc ataatttgtt tcttcttcag gtgttaacac tggattgacc
     4261 ttcacgaacg ccgtttccac tgtagaatct aaagaaaaaa gaacatcaaa aagattctta
     4321 tttgtcccag aatcttttaa tacgcttaac ccagtaaaaa taaggatcgt cataagaaag
     4381 acacggagtg cttgtttcat tcgagcctct ccagcatatt tgattgccat cataattgcc
     4441 gaaaaactga ttccaatagt tcctgcaata cttaacatat tgtccgcaat tttttgtgtt
     4501 aagttttgaa tgggttctcg aatggcggaa ataatatcta attcaaagag aaattcaacc
     4561 attgaagcat tgaattctcc taacattttt acgccactcc aaacaaaatt tttcatcact
     4621 cctgcaacat tgtacgatag ctttttttta aggttcattg actcatagtc attggtcatc
     4681 aattcaaacg aattcttttt gtaattttta tacaattgat ccacatcagt cgttacttct
     4741 tttacttcta ctttatcgtt ggcattctct ccttcagaac gatcggtttc agtgacaccc
     4801 gcaccatcat tcatttgatc ggcagaaaca gtagcccctt gtagacagaa aaaaagaccc
     4861 cctaataaaa ggagcgtcca ccatttcaga gatttttttc gcaaaaaaac cacctctttt
     4921 ttgttttatc gttgtttttc attttctaac attgcttgaa ttttttcatc ttgattttca
     4981 gtagaagaaa cagccaataa ccaactatca aacaacacat caatggcaat cggttgattt
     5041 cttcccaaat gatcctggaa taaacaggtt cctgtttgta attgttccat catttctaaa
     5101 ttggctgaat tcacttccat atcaaagaag gacaatagtt tctctcgtgc ttctttttgt
     5161 ttcggacgaa acgcaaattt gtaagaaatt aactcttttt tatcttcttg atcgtaatct
     5221 ttaaacgctt gcacaacaag gtaaatatcc gtattattcg cacgtccttt tcgcaagcta
     5281 tcatcaatca aatagcttcc ttgtgcggta tcttcatacc cttttgcctc gtcataaata
     5341 atcgcagcgt cttcttcttt gtttgtagaa aagatatacg taaacttcat aatgacttcc
     5401 ataattgcca ttcctgcaag ttgttcgcta tttaatcgac ccgaatcaat ctctttttgt
     5461 gtcggaatgc gtattccttg tgtccctaac acgttgattt gtgaatcaaa acgtatcggc
     5521 gtactgtgct cattcccaat gagaatcttt cctaaaccaa cgctatgccc taaaatcaat
     5581 ttagctagtt ctttgtcact ttttcgaatt tcttcgataa cttgacttaa cgaatgctta
     5641 cctggcatgg tgtaaactac atcgatggcg tcttggatca ccgttttctt gtggctcgct
     5701 gtttctcgat tggtatctac ttctcctaac attcctaaca catttttagc ggtttgaatt
     5761 gcctcttctt ttggtaaaaa caatagagga tcaagcatcc ctcgacagct ttcatctgaa
     5821 gataacgaaa taaaattaat tcgttcatac atagccacaa actctggtat gtgtccgaat
     5881 ttttcaagtg cctttaaaaa gaacttttgg gtttcatttt tcggatcaat ataaagaatt
     5941 ttttgaccta aaaacatcag ccataaaaaa atatatttta caaggaccga ttttccttta
     6001 cctggtggac caataatcaa cgtatttcca ttggtatgtt ccgcgccttc aatacttttc
     6061 tttgttaaat tgggataata ccatactagt ttactagata gcgctaacgc ttctttcgca
     6121 gatttaaact tttttgcaat aacaatccgt cctaaaggaa atcctttctt atttcctact
     6181 tctttcttta agtccaagcc ataatctgcc acaaaacctg tcgtagcgac ctgctcgtaa
     6241 ctacgaaaac gttgcgtact tccaattaag cattgattaa acaacgttag ctgatccgct
     6301 accgatcgat aaaatttgaa atctgtagac tcaatcgcaa attcaatatc tttgactcgt
     6361 tcttccaatt cttcttttga agaagcagat acaaccagcg tgacggtaga ataacacaat
     6421 cgtttttctt tgcgcttcag cttatctttt acatccatta accgttctgt accaaaattt
     6481 aatacatcgt ctgaatctaa aatgccgtca acggtctcat attctttttc ttgttcataa
     6541 atccgtccag ccattttatt aatttttcgt tcatcagatt ttgaatgctc aaatcgaagt
     6601 cgaatatgcg tatcaattgg aaaagataag ctatcctgaa gattttgaat aaagctactc
     6661 ccatacatgg ctaaaggagc gtccgtttga cttaaaaacg tcgaataatg cgtttgattc
     6721 aactgttcaa tcgcaatgta tcctgcttca ttcgaaatga cgccctcgca aatttccatt
     6781 ggcgtaagct gtctttcagg caatttttct tctgatcgat ggaaaaaata ataataaatt
     6841 tgtccaatct cttgggcaga taagcgctct atcggcttat aattggacaa ttccgtatag
     6901 agctcctttt ctaatgtttc atacgttggt aacacactac ttataggaac tcgttgtccc
     6961 gtaatacgat agataaactt tttaaaaaca tgcttaagca attgaaaata ttcgatcgga
     7021 tcagacactt tcatttctgt agtgaaacgc acaaacagat acgttcgata ctcgtttaat
     7081 tgcacttcgt cttttaaaat ctttcctgcc tgttcaaagt acactttccc aagatctgcg
     7141 cattctccct ttaccaaatc tcgaatggtt tcttcaatgt gcgtatctag gttaaattga
     7201 cgtggaatat tgataaaatg atagtcatat tcatcatgtc caagtatgcc ttcgccatct
     7261 tgaagatagt tcttgaaaaa atcaatgtca tttaaaggaa atacttggga agaaagttga
     7321 tagcctaccc acacatcatc atttttggtc aaaacaaaat ttcggataat ggcgtctatc
     7381 ggaaattcta atcgaggaag cttcgttttt ttctttttga actgccaaat cccgataata
     7441 aagacgacca aaagaaacaa aaacgccatt tactcgactc actccttcca tttgtgtagg
     7501 gaatacgtca ctttttcatg gaaaaacggc acatacattt catgttcaaa gcgttgtttc
     7561 cgtttctgat aataggacgt tcgcccaatt aaatactcga aaaagtgttt attgtcccat
     7621 ttcattgaaa acaatgtcca aacgagaaca ccaatcccaa aaatcataat caaccaggac
     7681 tttcgaaaca gtgtctgcaa gaaactaatt tgattcacat acgcaatcaa aaataggatc
     7741 aaaagaatga agagaaatac cccacctatg actaattgtt ccatgcgaat gccattagaa
     7801 atccagggaa ttttttgtcc tttaatcccg taaattttgt gagggtgctt aaactccttt
     7861 gtatagttgt attccatgtt cactctacct ccttttacaa aaaaagaagc gaccagaagt
     7921 tctaatcttc tgatcgcttt tgcagtttgt ctttttctta tgataattgt atccagggca
     7981 caatcatttg cgttaccccc acgttctctt cactatcctt aataaagata atgttcttta
     8041 gccattcacc aatatcaacg gcattcgata acaataagac gagcattatc aaaataaaga
     8101 cgccaataat ggcaaacgct gcccgcgcaa agccttgggt cgcataagca ccaaccatta
     8161 gaacaatcgc aacgattgcc aatagccaaa tgacttgtcc agatacatca tcaaataacg
     8221 atttaagatc aaaaagtttc atttccattc tcctctaagt tcattttatt catttaacgt
     8281 agtaacaaaa taactatcgc cacttgtttt tacttccatt gaaaagtttg attggaactc
     8341 gtttcctttc tcaaaataga aacgatactt cccttgcaaa aagtagtttc cttctttatt
     8401 ttttactgca tgatccagtt gtacattgat caattcgccg ccttctaacc ctttttcgtt
     8461 agaaatcaat gccaattttt catcattttt gacatataac tcaaaaaacc gtttcacaaa
     8521 tgcttccagc ttgatttctt cgctttcagt gaccgcttgc ccagaagaat aaaatcgatt
     8581 ttcctgatag ttcacagaat tggtattctt actctcagaa agattcaaaa attgaacgcc
     8641 atcaagtact tttaaaacat tgttttgata actgactgga aaagaaagtt gactgctttt
     8701 ccaatgttct ccttctgtaa aagtaacctc ataaaatagg cgatacattg cttgtgtcaa
     8761 atcgatttct tctagtttaa taaagcgaac atctttcgct actcgattgg tagccgtcgt
     8821 tacaaagcct agatcagacg cttccaatcc ttgtgctaaa taatgattga cggaagcctc
     8881 ccattcgttt cgtttttctg gcgaataagt aaataacctt tcaataaaat gttgtccctc
     8941 gtacttcaat tgttccgtag aagacaaggt ttggttgact tcagttaaca atgtttctgg
     9001 attgacttgt tttttcactg ctaaacgcgt taatgtatcc actcgtccaa agtttaggag
     9061 gagcacgaca cccattaaca gaaacaaaca aaaaaaagcg cctgaaaata cactcatggc
     9121 acttttttgc gaaaattgga tcattcgatc tgacggttct gttttctttt tttggaacaa
     9181 aaaacgtttc ttttgtttct tttccttctg ttgtttcggt ggcttagttt ggaacgcttg
     9241 tggattaaat tttgcattac tcatgagaaa tgccataccg ttctaacaat aatttcggta
     9301 ttttgtccac aatcactact tcgtggacaa ccacacttgc cccctccaaa aaggcaagtg
     9361 cttctttgta ggcattttga ctgtattttt ttgataaagc atgacaaatt tcttgataaa
     9421 atagttgact gcctaccgaa taccgattat tgtgtatccg ccacaaaacg aacacactga
     9481 tttgaatcga cgggcgtaaa aaagaaaaat tctttccata agaaaaagaa cgtccttctt
     9541 gggtttcttt tgcttgttct gttggtactt cttgtacctc tcttattgat tcttgtctct
     9601 ccataacctt ctccttttct tattcttcta aaagctgttg attaaagcta cttcccaaat
     9661 tttgcataaa cgtgattttc tttgacgctg gatcaaaggt tacgtcaaaa atattcatgg
     9721 cattattcca ggaactatca ccaattttat tgcgatacga caccacaacg agcccttgga
     9781 tagtcgcttt ttgtgtgctt tttaaataaa cagaacattt ctctaattca gaagacaccc
     9841 cgtttgaatc accataggaa acgccttctt tgaaatattg cttcatcagt ttgtcagaaa
     9901 gatatggtgc acaattgttt tttcgttctt gaaaagaatc aggctcataa ttaaacagaa
     9961 cctcgaacgc ttctgtcact gttttttgaa aggtttgaat gtttcctttt tctgttgaat
    10021 cattgttttt caattcttca tttgcttttt ttaacgtcgt gacctcgctt tctacttgtt
    10081 gaagttgttc tttgagtgac gttacaaggt cttcctgtcc tttcttctct tcttggaact
    10141 tgtttatctc caccgtcagt ttctcttcct ttgctgttag ctgtagcttt tcatgaataa
    10201 gaaaaaatgt actgcctaca ccaaataaga acaatccgcc tacacaaaca taaataaatc
    10261 gtttcttcat ttgacacctc ttatttcttt atcggtcgag caaaacaaat atcaccacca
    10321 ttaagcaaat acccaaagga taaattgact tgcccttcac ctacaccgtt agtattcatt
    10381 cccccttgtt cgattacttt cgtagaaagt ccatgccaat cttccgctaa aacagctgtg
    10441 tgtccatcat ttcccacacc agaacctaag ttcacaatga taatatcgcc agcttttgcc
    10501 tcgctctcag gtatttcttg taagtattgc ttacttcctc ttgcgtcgct cgtcattgag
    10561 ccggtaaacc acccaacgtt cgcaggcgtt ttataccctg ctttcgttaa aacaagccac
    10621 acaaagctcg aacaatcggc atagccattt ttatctggat tttcgacact accaaattgt
    10681 gcccgttgcg gttgtgaata atgaaaataa ccaatcagac tttttgctac gtttaataca
    10741 ttactatcgg aagaactgct tgttcctgtt gtttctagac aataatcggt caagtcttta
    10801 cttcctgcgt ttgccccatt ttctaatgta attgaagcga cgggatcact gtcattcttt
    10861 cctagttttg cgtaccattg attcgcaaaa gtaatacgat tccccatcat tggtatccct
    10921 gcacgctcaa atttcacttg ccactgttct gttgcttcct ctacgctttt tgcttgtaat
    10981 gcaacttgtg aagctttttc tgttgtgttt agttcatgaa tcaaaaactc aacttgtaca
    11041 cctaaatctg accactcttt gtttcgctct ttggcaaaat ttttcaaagc agtcgttcgc
    11101 cctgccgtcc attgtgcaat cccatgccca gataatggat cgtttggtcg ttcaatcgct
    11161 gtgggactca tactcgactc ttgttgaaaa ttgcctaaca tacccgctag accctctaat
    11221 gtcgtatttg gaatatgttt ttgtacttcc tttgcaattt gaagcccgtt tgtctccatg
    11281 ccttggttcg ttaatgagat agtacttgcg acaatttcgg ttggactaca gtcgatttct
    11341 tcctcttcag aaccactact aatcaaaagt atcataaaca gaaaaccaaa catacatagt
    11401 aatagtgtgg gtagacaacc gaacccacac cataaaagaa gttttttctt catgtttgat
    11461 ccttcattcg cttagttcat tttttgtgtt gcttttcttt tgcgccaaaa agcaaaaccg
    11521 cttcctgaag ccaataatcc accaagtgct gtcgcaacga gagacacttc ttcgccagtc
    11581 ttaggcaata ctttcatttt tccatcagac cctttcaccg caatatttcc gcttggaagt
    11641 tgttcgacct tgtctgaaat gggcgtcaag ccatttcctg attctttata agcgacacca
    11701 tcaacgacgg agacaatttt ttctcctgaa gctgtttcaa ttggtgtact tggtgttgga
    11761 atatgggtta attcagacgc attattggtt tcaatttgaa cattttgtcc acctgtttgt
    11821 tggctgccaa tacttccatc tggttttact acgacgtcct gtactggagg cgtagtgttc
    11881 gtccccccat tattggtagg tggtgtcgtt ggaggagtcg tatttccttc attgcctcct
    11941 ggattcgacg gcttttctgg tgtctctgta ttaccaccat ttcctgggtt ttcctcaacg
    12001 ggtggaactt caggtgttcc ttcccctccg ccagtatctg gattttctgg tacttcaggc
    12061 gtttctgtgc ctccacctgt atctggattt tcttccgttg gtggaatctc tggggtttca
    12121 ggagtttctg gtatttcggg aatttctggg gtttcttctc ctccatctgt tggtggtaaa
    12181 acaggttctt caggaacttc aggtgtttct tccgttggtg gaataatttc tgtcggtggt
    12241 acacttggat cacctggtga cgtagtatct gcatacgcac tggttcctac catacctagc
    12301 aatacagttg cactaaataa acttcctaaa atcatttttt tcatgtgttt ttcctcttct
    12361 cttatttatt gatttatttc aaaatcatct a
//
LOCUS       DADXTG010000076        12131 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.077, whole genome shotgun sequence.
ACCESSION   DADXTG010000076 DADXTG010000000
VERSION     DADXTG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.077"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..578)
                     /gene="frr"
                     /locus_tag="IWI57_001797"
     CDS             complement(21..578)
                     /gene="frr"
                     /locus_tag="IWI57_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBC2449937.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(581..1303)
                     /locus_tag="IWI57_001798"
     CDS             complement(581..1303)
                     /locus_tag="IWI57_001798"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBC2449938.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1439..2320)
                     /locus_tag="IWI57_001799"
     CDS             complement(1439..2320)
                     /locus_tag="IWI57_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBC2449939.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="IWI57_001800"
     CDS             complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="IWI57_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBC2449940.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3560..4039
                     /locus_tag="IWI57_001801"
     CDS             3560..4039
                     /locus_tag="IWI57_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBC2449941.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4256..5347
                     /gene="phnW"
                     /locus_tag="IWI57_001802"
     CDS             4256..5347
                     /gene="phnW"
                     /locus_tag="IWI57_001802"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBC2449942.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5340..6125
                     /locus_tag="IWI57_001803"
     CDS             5340..6125
                     /locus_tag="IWI57_001803"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBC2449943.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6471..8162)
                     /locus_tag="IWI57_001804"
     CDS             complement(6471..8162)
                     /locus_tag="IWI57_001804"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBC2449944.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8584..9531)
                     /gene="arcC"
                     /locus_tag="IWI57_001805"
     CDS             complement(8584..9531)
                     /gene="arcC"
                     /locus_tag="IWI57_001805"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBC2449945.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9646..10665)
                     /gene="argF"
                     /locus_tag="IWI57_001806"
     CDS             complement(9646..10665)
                     /gene="argF"
                     /locus_tag="IWI57_001806"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBC2449946.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10756..11985)
                     /gene="arcA"
                     /locus_tag="IWI57_001807"
     CDS             complement(10756..11985)
                     /gene="arcA"
                     /locus_tag="IWI57_001807"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBC2449947.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3319 a         2529 c         2183 g         4100 t
ORIGIN      
        1 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
       61 ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      121 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      181 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      241 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      301 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      361 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      421 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      481 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      541 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      601 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      661 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      721 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      781 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      841 cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      901 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
      961 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1021 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1081 aatcagcttg agcacgttcc atgcccatat gtgctccgat ttgtccgcgc cagatatttc
     1141 cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1201 tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct aaagcttctc
     1261 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1321 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1381 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1441 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1501 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1561 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1621 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1681 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1741 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1801 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1861 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1921 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     1981 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2041 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2101 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2161 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2221 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2281 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2341 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2401 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2461 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2521 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2581 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2641 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2701 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2761 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2821 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2881 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     2941 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3001 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3061 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3121 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3181 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3241 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3301 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3361 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3421 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3481 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3541 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3601 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3661 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3721 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3781 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3841 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3901 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     3961 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4021 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4081 tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac atcatttttt
     4141 attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4201 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4261 taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4321 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4381 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcgg
     4441 ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4501 ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4561 ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct
     4621 gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4681 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc ttttgtttat
     4741 agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac tagggattga
     4801 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4861 tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt cacttgatct
     4921 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     4981 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5041 aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa tgacagagat
     5101 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5161 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa acggatttgt
     5221 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5281 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5341 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5401 ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa
     5461 tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5521 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5581 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5641 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5701 cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaagggt
     5761 attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga ccgtttcctt
     5821 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5881 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     5941 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6001 ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct ggcgctgata
     6061 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag
     6121 aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa gtaataagaa
     6181 cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6241 aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6301 gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6361 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6421 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6481 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6541 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6601 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6661 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6721 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6781 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6841 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6901 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     6961 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7021 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7081 ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7141 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7201 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7261 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7321 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7381 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7441 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctcttcatcg
     7501 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7561 gctacttcat ggaattggac atataaacga agcaattcat tgattggttc tgctttaaca
     7621 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7681 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7741 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7801 gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc ataatgttct
     7861 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7921 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     7981 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8041 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc gacttgttcc
     8101 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8161 atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8221 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8281 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8341 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8401 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8461 tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc agatggccta
     8521 aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac
     8581 aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc
     8641 taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcctc
     8701 tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt gtttcatttc
     8761 tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga catacacatt
     8821 gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga
     8881 agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac
     8941 aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat
     9001 gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg
     9061 tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc
     9121 ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc
     9181 cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac
     9241 ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg caacacatgt
     9301 atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt
     9361 accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt
     9421 cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt
     9481 agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca tttcattcgt
     9541 cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt
     9601 ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat
     9661 aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc
     9721 ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt
     9781 gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg
     9841 caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt
     9901 gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga
     9961 tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt
    10021 aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc
    10081 ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt
    10141 gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc
    10201 ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg
    10261 ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac
    10321 cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta gtactttagc
    10381 tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg
    10441 atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt
    10501 ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt
    10561 caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc
    10621 tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct
    10681 ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagaccg
    10741 ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg
    10801 accaccacgg cctcgtgaca attcacttga attgatttct aacactttga tgccatagct
    10861 acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat
    10921 tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt
    10981 accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa
    11041 gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat
    11101 cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt
    11161 aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg ctaacacttt
    11221 ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt
    11281 agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc
    11341 ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct
    11401 tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt
    11461 catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa
    11521 gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat cgtatagact
    11581 gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat ctaccatttc
    11641 ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat
    11701 atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc
    11761 ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtctttgc
    11821 aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg
    11881 atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca gtaaaactgt
    11941 ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct
    12001 tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc
    12061 attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa acgtttttct
    12121 atttttaagc g
//
LOCUS       DADXTG010000077        11890 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.078, whole genome shotgun sequence.
ACCESSION   DADXTG010000077 DADXTG010000000
VERSION     DADXTG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.078"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            21..800
                     /locus_tag="IWI57_001808"
     CDS             21..800
                     /locus_tag="IWI57_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBC2449948.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            793..1110
                     /locus_tag="IWI57_001809"
     CDS             793..1110
                     /locus_tag="IWI57_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBC2449949.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1354..2964
                     /locus_tag="IWI57_001810"
     CDS             1354..2964
                     /locus_tag="IWI57_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBC2449950.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3121..3969
                     /locus_tag="IWI57_001811"
     CDS             3121..3969
                     /locus_tag="IWI57_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBC2449951.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            4200..4679
                     /locus_tag="IWI57_001812"
     CDS             4200..4679
                     /locus_tag="IWI57_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449952.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            4712..6940
                     /locus_tag="IWI57_001813"
     CDS             4712..6940
                     /locus_tag="IWI57_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBC2449953.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            7069..8712
                     /locus_tag="IWI57_001814"
     CDS             7069..8712
                     /locus_tag="IWI57_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBC2449954.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(8785..9330)
                     /locus_tag="IWI57_001815"
     CDS             complement(8785..9330)
                     /locus_tag="IWI57_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBC2449955.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(9353..9838)
                     /locus_tag="IWI57_001816"
     CDS             complement(9353..9838)
                     /locus_tag="IWI57_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2449956.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(9828..10640)
                     /locus_tag="IWI57_001817"
     CDS             complement(9828..10640)
                     /locus_tag="IWI57_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBC2449957.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(10779..>11890)
                     /locus_tag="IWI57_001818"
     CDS             complement(10779..>11890)
                     /locus_tag="IWI57_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBC2449958.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         3837 a         2206 c         2378 g         3469 t
ORIGIN      
        1 taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc
       61 atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt
      121 tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg
      181 gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat
      241 tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt
      301 ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa
      361 actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt
      421 tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt
      481 tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca
      541 ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag
      601 gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa
      661 cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta
      721 agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg
      781 aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg
      841 gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg
      901 ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat
      961 gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt
     1021 ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt
     1081 gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg
     1141 cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg
     1201 caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta
     1261 ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt
     1321 ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca
     1381 ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc
     1441 tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt
     1501 ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt
     1561 gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt
     1621 agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta
     1681 tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct
     1741 atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat
     1801 ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg
     1861 cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata
     1921 ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc
     1981 catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc
     2041 agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta
     2101 ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg
     2161 aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc
     2221 agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca
     2281 gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc
     2341 gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact
     2401 ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt
     2461 gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta
     2521 tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca
     2581 attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg
     2641 agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc
     2701 aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc
     2761 tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg
     2821 tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc
     2881 ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa
     2941 catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg
     3001 gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat
     3061 cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac
     3121 atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat
     3181 cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga
     3241 atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact
     3301 acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg
     3361 tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta
     3421 tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc taagatcact
     3481 agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc
     3541 cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctacgc ttatctgatt
     3601 gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt
     3661 ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc
     3721 gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga
     3781 aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta
     3841 gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt
     3901 catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca
     3961 gcgcaataat tccttttatt caaaaaacat accaaaaata ttccaacatc atcttggact
     4021 ttctacatgt tactaatgag cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg
     4081 aagctctttt tctttgcaat cctcacttca atcctctata atgtcgcata tagtttagta
     4141 aatgagtaaa ctactaattt acttatttac taaaaataaa aggatgagga gttaaaataa
     4201 tggaaatacc aacaaattta aaacataaac cagtaatcgt tgtagaagac tatgataaag
     4261 ttgatggaag aaatgcatta aatacggatg caaaagggct ttcactgggc cttgctcagt
     4321 ggaatgatcg tggaaaagtc gatatctcag gcaaggtatg gcgacatact ggagaaaaat
     4381 ggtcacgaca gtcggaagaa ttaccaatca cacgtatttt agatttagcg atcttagctg
     4441 cacaaggaag tttatacttc caagatgctt atcgttatga aaaattttat gatccagaaa
     4501 atccagtcgt agacatcatt ggattacaag ggaaccgaat gacggtagaa gtagatacca
     4561 aaaatccaaa gatcgatgaa gacatcattt tgtattacga tacactacaa aaagacggag
     4621 aactgattgg ggaacgattc cgtattttaa aacgtctttt agatgagcta ggatattaat
     4681 agacagagat gatcaaaagg ggaagagaag catggagaat ttctcactaa cagaaaaccg
     4741 aaccttttca cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag
     4801 tgcagaagaa tggcggatcg cctccgaaat caaagggaat tggcaagatc ctgaaatgaa
     4861 aatcagtaac gtacttttgg aaggagatgc cgggtcgggg aagacacaat tagccaaagc
     4921 actatctgct gatctgcaac ttccctatac aaaagtgacc tgttttgcag atatggacaa
     4981 atcagatgtt ttcggagcct tattacctgt agtagattct aataaggaac aagaccaaga
     5041 attactagaa gctatttacc aaacagatac acttgaagct gtactgacac tgatccagaa
     5101 ccattacggc attgatcctt tttctgctaa agaaaaatta gcagagctag ttcaaaggat
     5161 cgaagctgat tatccagatg caatccatta cagctattac ccttctgaaa tcgttcgagc
     5221 gattgaaaaa ggatatttat tagagattca agagccgact gtcatccgag atgcctctgt
     5281 tcttgtggca cttaactcag cgctcgaacc aaatgggatg ttgaatttac cgacaggaat
     5341 cattcgtcgc catccagatt gcgtcattgt gatcactaca aatcgaaatt accaaggaaa
     5401 tcgcccatta aatgaatctt taagagatcg gatgcaacat gcggaaaaaa tggatcttcc
     5461 tgccttagaa gtcatggcag aacgtgcgat ggcgaaaact atgatccaaa aacaagaact
     5521 tctcttaaag atggcagaga ttatccgttt gttggatgaa actgcaaaag ctaacgccat
     5581 caaaggtgta gcaggaatgc gttcttattt ttattgggca aatacctata agcaaggaca
     5641 agatttactc cagtcgattt atccaaaagt tttgtataaa ttatccactg attcagatga
     5701 attggcaatt cttgaacaag ctttgtctga cagtggattg ctactggaac ttcaggacat
     5761 attgagaaaa gaaaaatggg gtcatcctca atctgagcat acaaaaggaa gaaccattag
     5821 cgccgaagaa gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga
     5881 tgaaaagaaa tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac aagaagaaac
     5941 aatagaaaaa aacgagattc ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg
     6001 aaaaaatcaa aaagaacaac aaacaggaga tgattctcaa gaaatgtctg cttctgacat
     6061 ggaagcacat gataaggaaa tcaagaagca actgaataaa gaagcgcgac aaatcatgaa
     6121 ggaaacgatt catgaaaaag aaggtctcat cgttcaccga ccagctttcc aaggaaaaac
     6181 gccggaagca cgtgagctac acatgcaagt aatgccaata gtagaatcac ttagccggca
     6241 aattttagag ttactggata atgaacaaac agagacgtac caaaaaggaa aatacgaagg
     6301 tcagcggttc aatgcaagca aagtagcata tggtgatttg agaaattttg acaaaaagaa
     6361 cccgccacat gagcaacctt ctttagcggt agctgtacgg atcgatgaat ctgggtcaat
     6421 gatacgggat gatcggatcg aagcggcaaa aaaagcagcg attgccattg ccgaattttc
     6481 taagaaagtc gatatcccat tgttgattta cggagataca gcagaccgtt cgtcaagaga
     6541 aaagacttct ttgttttctt ataaagaatt tgaagacgga tttcaatggt taaatgaaaa
     6601 attagtcact atgaaaccaa ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc
     6661 agaaaaatta aacagacaat ccgcaacaac caaactgttg cttaatatta gtgacggtca
     6721 gccaaaagcg ttgccggatt atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt
     6781 gaaagaatat gaacgtcaag gaatcctatt tgtctccgca gctattggcc aagacaaaga
     6841 agaaatcaag tcgatctacg gtgaaagtcg ttttgtagat attactgact tgaatgaatt
     6901 tcccaagcaa atgattcaac tgattgcccg ctatctatga taaaataaag ctcaacgaac
     6961 gcacttagca gattcgttga gcttttcgtt tctagttacc ggtaacaagg atacttctaa
     7021 aatagaatcg gttataatca agagggaccg ttaaaggagt gaaacgatat gatacaagaa
     7081 caaaaatggt ggaaagaagc agtaggctat caaatctatc ctgctagttt caaagatagc
     7141 aatggcgatg gtcaaggaga catcaatggg atccgtgaaa aacttaatta tttgaaagat
     7201 cttggtatcg gctttatctg gatcaatccg atctatcaat ctccatttgt cgataatggt
     7261 tatgacgtaa gtgactatca agatatagaa gagagattcg gaacgatgga agattttgat
     7321 ttattgctaa aagaagcaca tgaattaggg atcaaagtga ttatggatct tgtcatcaac
     7381 catagttcgg atcagcatca atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat
     7441 cgcgattatt acatttgggt cgacggggta gatggcaaag aaccgaataa ttggacatcg
     7501 attttcggcg gttctgcttg ggagtattct catgaaactg gacagtatta tctgcatgtt
     7561 tttgcaaaag aacaaccaga cttaaattgg gaaagcgaga aattgaaaga agaactattc
     7621 aatatgatcc gatggtggtt ggataaaggg atagatggat ttcgtttaga tgcgatttct
     7681 catgtaaaaa aagatgaata ttcagtaaaa gccacagaga accctttctc accctttcaa
     7741 aatgtctcag gaatcgaaga acatttgacc gatttgaaac atgtttttga agagtacgat
     7801 atcatgactg tcggagaagc aagtggtgtc actgcagaag aaggaccaca atgggtaggg
     7861 aaagacggtt atttcgatat gatattcgaa tttgatcata tccatatctg gcagcaagaa
     7921 aaagaagggc aattagatgt ccttaaattg aaacttgcat taagcgcttg gcaaacttca
     7981 ttggatggta tcggatggaa cgctttatat atggaaaacc atgatgttcc tcgtgccgtt
     8041 tcagtgtttg gagatactcg tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg
     8101 atgtatttct ttttgcaagg tacgccattt atttatcaag gacaggaaat cggaatgacg
     8161 aatatgccct ttgaatcgat cgatcaagtt gatgcagtag attcgaaaag actttataaa
     8221 aggctgcttg cggaaggtaa gacaagagaa gaagcattgg atatcattcg tgaaacaacg
     8281 agagacaaca gcaggacacc catgcaatgg acttcggaac aatatgctgg tttttctaca
     8341 catgaacctt ggctgatcac taatccaaat accaaaacga tcaatgtaga acaacaagaa
     8401 tatgaaccag agtcggtcct gcaattctat aagaacatga tccgcattag acaaaccaat
     8461 aaaggtctga tatacggttc ttataaagaa tatcttcatg aacatccaca actttatgtc
     8521 tatgaaagat atttagaaga tgaacaatat cttattatgg taaacttaac agaatcactt
     8581 gctgactatg aacttccaaa agaagcagat cagtcctgga cattactctt gtcaaattcc
     8641 tctagcggag aatttgaagc taaaggcata ctagcacctt atgaagctcg gttatataaa
     8701 acaaacaagt aaaacttcct cgttaaagtt cattagttgg actttgcgag gaagtttttc
     8761 agattgctct gtcaattact catttcaata attcgctcta ggaccaccga tatatttagg
     8821 acgagagcga agggctgtca cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg
     8881 tacttggaca tgtttgatat gcataccgat tgatgtgtca ccgatatcga taccggcttg
     8941 tccaactacg tgttccactt cgacaggatc attgaactgg tcaaaagcag caacagaaca
     9001 agcacctcca gcatgtagtg caggaacaac agagacaatt tcgaaatttt ttgcttctgc
     9061 cactgttcgt tctactacta atgctcggtt caaatgttca caaccttgga cagctagcgc
     9121 aatttcttta ggatccaaca actcttttaa cgtacggatg atccattgac caacttccgc
     9181 actggaattt tttccaatat gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa
     9241 aagatcacct tttttcaagt gtgcctcagc caatatgtca ttaactattt cagtcaattg
     9301 gtcttttaat tctttttctg acaacttcat tttctaaaac cttctttttc aattaatatt
     9361 gcttccgtgc ttgaatacgt tgaagacttg cagttatagg aaaggcaagt cccattccaa
     9421 tcagattttg gaagatgttt cctggtatag aagccactcc agccggccaa tcatataaaa
     9481 tccaaccggc gataccatag ccgatgatca taactaagct tcctaataaa agccctaatc
     9541 cccacatacg agtagaagat ttgctgaaag aaccagcaac atatccttgt aacccgtgga
     9601 tcactaatga aaaaactgcc cactgcggat atcctgaaat caaatcgatc aatagaccac
     9661 tggaagcacc aacaactaat ccacccgttg gaccataaag aaaagcagcg gtatagatcc
     9721 cggcctcgca taatgtcaca aatccattcg tagccggtac agggatcaaa attgttaatg
     9781 aaagggcaac tgtcaacgca atcagcatag catagatcgt taattgctta gttttcattt
     9841 ctcattcctc ctttttgaat accgccagac cagacacttc cggtatgatc gttcatcagc
     9901 acgccattag aaatacaatg ggcaacaaat tttttacttt ctgctactgc tttggtaaaa
     9961 tctgccccta acacaagatt cgctgttatt gcagaagaaa aggcacaacc ggccccgttt
    10021 actgttgtag tgtcgatttt tttatgagaa aatacctcat aatgatcaat tgaatagaga
    10081 agatccacag cttcatctcc agccatcctt gtgccgcctt tgatcacaat gttttttgtt
    10141 cctgaagcag tgagttcttt agcgagatgg atcatttctg ttaacgaaga gggaagagct
    10201 tgaccagaaa gtaaagcagc ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa
    10261 agttcttcga tgagctgcga aatatacaat tgattgatca cagcttcagt ttctttgaat
    10321 gccaaaacag ggtctaaaat gattgggata ttttgttttg tttttatgaa gtctcgaaca
    10381 accatgattg cttcacttga attcaataac ccaattttga taccatctaa tgaagtcatt
    10441 ttatcaatcg aaactaactg tcgtttaagc aattccaaag atatagattc cagcataaat
    10501 cgctcattaa tatctagtgc ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa
    10561 ttctcaaaag tttttaaatc tgtttggatt cctccaccag caaatggatc agaaccgcca
    10621 attgtcaaga ttcttttcat aacattttcc tccatgaata agagtataac gaaattttca
    10681 aaaaagaaaa acagccaatt ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga
    10741 aaagaaagac caaaatggat ctttctttcc ttaaatagtt aggacttttg tgattttaat
    10801 tctttctcga tttgaagccg ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa
    10861 tcacggatga tagggacgtt caagcgagga agttcatgga cttgctccca ccatcgattc
    10921 caatccattt ctttatcagg agctaaagca ataatcggtt gtggagaatg atttttatcc
    10981 cttacaatca ctacttctgc caaaaaatct aacgaatcta ataaaaagtc ttcaatagct
    11041 aatgtactat tgatcgtttc gatcaaatcc acttgccgat ctttcaaaaa taggtggcca
    11101 tgcttatcca taaaaccgta atctccgcta tcccaccaat cgccataaac attttcctgg
    11161 aaccgtgcat cttccttgta ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga
    11221 ccatctgtgt tttcaggtag gacattccca actgaatctg taatccgagc gcttgtcatg
    11281 tcttccagtc caacgcccat atctcttgca tcacttgttt tcaaactctc tagtgtatga
    11341 gccttaagaa tcattggtcc gcattcactt tggccatata cttgaagaaa taccgcatcc
    11401 tgtgccatcg acgttcgcaa aaatgtcttc attgtctggt tgtttatagc atcaaaagtg
    11461 gagtgatagt atcggatacc cgaaaaagct tctggatgct ccttagcagt aaaacgccac
    11521 tgaacaaagt tattcggatg ggtctctagt gcaattggcc ggtgagcttc caacatatga
    11581 acaacttttg aactttgtgc gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa
    11641 gaagagatac caatattgaa tctggaatgc actggagaga tgtgaaaagc gattaatttc
    11701 ttttccgaaa tttttgtgaa aactgttttt tgccatttcg tccgccatcc catagaatga
    11761 gcagaatgac aaataagttt agggatacca gttgtaccag aagtatgggt catataagaa
    11821 atttcatttt tgtctaaaaa tgattgagaa acagacaaag caggttgttc cattaaataa
    11881 agaataggaa
//
LOCUS       DADXTG010000078        11852 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.079, whole genome shotgun sequence.
ACCESSION   DADXTG010000078 DADXTG010000000
VERSION     DADXTG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.079"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..320)
                     /locus_tag="IWI57_001819"
     CDS             complement(<1..320)
                     /locus_tag="IWI57_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBC2449959.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLS"
     gene            497..1225
                     /gene="sfsA"
                     /locus_tag="IWI57_001820"
     CDS             497..1225
                     /gene="sfsA"
                     /locus_tag="IWI57_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBC2449960.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1241..2254
                     /locus_tag="IWI57_001821"
     CDS             1241..2254
                     /locus_tag="IWI57_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2449961.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2254..3531
                     /locus_tag="IWI57_001822"
     CDS             2254..3531
                     /locus_tag="IWI57_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBC2449962.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3594..6296
                     /locus_tag="IWI57_001823"
     CDS             3594..6296
                     /locus_tag="IWI57_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2449963.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6448..6765
                     /locus_tag="IWI57_001824"
     CDS             6448..6765
                     /locus_tag="IWI57_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2449964.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6795..7115
                     /locus_tag="IWI57_001825"
     CDS             6795..7115
                     /locus_tag="IWI57_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBC2449965.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7223..8683
                     /locus_tag="IWI57_001826"
     CDS             7223..8683
                     /locus_tag="IWI57_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2449966.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8751..8972
                     /locus_tag="IWI57_001827"
     CDS             8751..8972
                     /locus_tag="IWI57_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449967.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9003..9185
                     /locus_tag="IWI57_001828"
     CDS             9003..9185
                     /locus_tag="IWI57_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBC2449968.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9185..9598
                     /locus_tag="IWI57_001829"
     CDS             9185..9598
                     /locus_tag="IWI57_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBC2449969.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9721..10902
                     /locus_tag="IWI57_001830"
     CDS             9721..10902
                     /locus_tag="IWI57_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2449970.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10973..11137)
                     /locus_tag="IWI57_001831"
     CDS             complement(10973..11137)
                     /locus_tag="IWI57_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449971.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11291..11364
                     /locus_tag="IWI57_001832"
     tRNA            11291..11364
                     /locus_tag="IWI57_001832"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11325..11327,aa:Arg,seq:cct)
     gene            complement(11433..>11852)
                     /locus_tag="IWI57_001833"
     CDS             complement(11433..>11852)
                     /locus_tag="IWI57_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2449972.1"
                     /translation="KNETSNRKISIDKHTIDLLKKFKINQKELFENFEVKNPHNFVFF
                     NLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGH
                     SSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         3889 a         1995 c         2479 g         3489 t
ORIGIN      
        1 gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa
       61 aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc
      121 acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat
      181 tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga gagtccttct
      241 gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt
      301 gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc
      361 gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta
      421 agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga
      481 agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc
      541 gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa
      601 aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc
      661 tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg
      721 gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac
      781 gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct
      841 tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt
      901 caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag
      961 aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt
     1021 cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc
     1081 agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa
     1141 ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat
     1201 cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat
     1261 cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga
     1321 attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga
     1381 aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac
     1441 catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc
     1501 aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat
     1561 catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga
     1621 atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa
     1681 tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa
     1741 tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata
     1801 tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc
     1861 tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat
     1921 ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac
     1981 gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa
     2041 agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga
     2101 ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg
     2161 gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat
     2221 caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc
     2281 gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga
     2341 gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca
     2401 ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt
     2461 gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt
     2521 gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa
     2581 cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata
     2641 ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat
     2701 gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa
     2761 gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc
     2821 attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct
     2881 cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg
     2941 agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa
     3001 ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct
     3061 gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga
     3121 gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta
     3181 gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt
     3241 cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta
     3301 aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag
     3361 ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg
     3421 aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat aagaaaaaaa
     3481 gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata agcgcctttt
     3541 tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt tatgtgaaag
     3601 aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg acagcaagtg
     3661 atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta agtgaactat
     3721 acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct ttgccaacgg
     3781 aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact tttgaaacac
     3841 tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct gcgattcttt
     3901 atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg aagtcgatgt
     3961 ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca gatgcgccat
     4021 ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt ggacacatct
     4081 ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt ctaatagcca
     4141 aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg gaagggcagg
     4201 aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa agtgcacaag
     4261 tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa agtttcttat
     4321 ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct tcacgttcac
     4381 tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat cgtttgaatc
     4441 aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat gcggaaggaa
     4501 atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt ttacattacc
     4561 gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt caagtacaaa
     4621 aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg gacagcaaaa
     4681 gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa gatccagaac
     4741 gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc gaaaccggtg
     4801 tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat ttccagacat
     4861 atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat gaaatctggc
     4921 ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt tataatgaaa
     4981 aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg agagaaggtc
     5041 atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg gaattccagg
     5101 tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt ccttttgatg
     5161 aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag cctgttaaag
     5221 aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca agcatgttgg
     5281 agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg ccactggtta
     5341 tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat caagagcatt
     5401 tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc agtgatttga
     5461 tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc atgattgtga
     5521 tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat caaaatatcc
     5581 agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct gaaactttac
     5641 agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct gccaaattat
     5701 atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag atgcagtttt
     5761 tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat gagatcaaag
     5821 caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca gcctatgacg
     5881 tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag atcccgacag
     5941 ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt gatgaactga
     6001 tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt atcgtggaac
     6061 ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga ttgatcaaac
     6121 aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat cgtttggaaa
     6181 tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta aaaattccag
     6241 aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa aaatgaacac
     6301 actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa
     6361 gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt tatataaaag
     6421 tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca
     6481 gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg
     6541 atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt agaatctaag
     6601 aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca atttgaacaa
     6661 aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta tggcatgatg
     6721 aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa gtatgcagtt
     6781 gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg ttaatcatgt
     6841 ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt
     6901 ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa gcgcatcatg
     6961 cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg acattattga
     7021 ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta gcaaaagaaa
     7081 tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt tgaacggaag
     7141 aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa
     7201 aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct
     7261 gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat
     7321 cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg
     7381 tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat
     7441 ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc
     7501 aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt
     7561 aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg
     7621 tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa
     7681 tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg
     7741 gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat
     7801 ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc
     7861 agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata
     7921 cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga
     7981 tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt tgggagcagt
     8041 attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg gaccaaatgt
     8101 attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt
     8161 ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg
     8221 aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg
     8281 cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac
     8341 agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg
     8401 tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc
     8461 aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt
     8521 cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc
     8581 accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat
     8641 ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg
     8701 ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa atgattacaa
     8761 ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag
     8821 gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag
     8881 aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa
     8941 cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg
     9001 agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt
     9061 tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag
     9121 gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata
     9181 actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat
     9241 ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct
     9301 gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga
     9361 agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt
     9421 cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa
     9481 aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat
     9541 gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat
     9601 caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca
     9661 taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa
     9721 atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc
     9781 acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg
     9841 gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg
     9901 gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat
     9961 gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc
    10021 cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt ctcctatccc
    10081 ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt
    10141 cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat
    10201 gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg
    10261 aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct
    10321 ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct
    10381 tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc
    10441 ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt
    10501 aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg
    10561 atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa
    10621 gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg
    10681 gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca
    10741 atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa
    10801 gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca
    10861 atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac
    10921 aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta
    10981 aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt
    11041 tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc
    11101 gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc cctacattat
    11161 ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta
    11221 taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt
    11281 tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg
    11341 ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgtg gtacaggggt
    11401 ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg
    11461 aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaagataa
    11521 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
    11581 actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa agtaaattgc
    11641 ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc gtttgatgaa
    11701 actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttaac ttcaaaattc
    11761 tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt atgtttatca
    11821 attgaaattt tccgatttga agtctcgttt tt
//
LOCUS       DADXTG010000079        11515 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.080, whole genome shotgun sequence.
ACCESSION   DADXTG010000079 DADXTG010000000
VERSION     DADXTG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.080"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            199..405
                     /locus_tag="IWI57_001834"
     CDS             199..405
                     /locus_tag="IWI57_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBC2449973.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(607..1608)
                     /locus_tag="IWI57_001835"
     CDS             complement(607..1608)
                     /locus_tag="IWI57_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2449974.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1613..3526)
                     /locus_tag="IWI57_001836"
     CDS             complement(1613..3526)
                     /locus_tag="IWI57_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBC2449975.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3694..4200)
                     /locus_tag="IWI57_001837"
     CDS             complement(3694..4200)
                     /locus_tag="IWI57_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2449976.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4360..4800
                     /locus_tag="IWI57_001838"
     CDS             4360..4800
                     /locus_tag="IWI57_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBC2449977.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4826..5983)
                     /locus_tag="IWI57_001839"
     CDS             complement(4826..5983)
                     /locus_tag="IWI57_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2449978.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5986..6342)
                     /locus_tag="IWI57_001840"
     CDS             complement(5986..6342)
                     /locus_tag="IWI57_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2449979.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6640..7614)
                     /gene="sgrA"
                     /locus_tag="IWI57_001841"
     CDS             complement(6640..7614)
                     /gene="sgrA"
                     /locus_tag="IWI57_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBC2449980.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7810..8649)
                     /locus_tag="IWI57_001842"
     CDS             complement(7810..8649)
                     /locus_tag="IWI57_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBC2449981.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8834..9721
                     /locus_tag="IWI57_001843"
     CDS             8834..9721
                     /locus_tag="IWI57_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBC2449982.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10305..11000)
                     /locus_tag="IWI57_001844"
     CDS             complement(10305..11000)
                     /locus_tag="IWI57_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBC2449983.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(10984..11382)
                     /locus_tag="IWI57_001845"
     CDS             complement(10984..11382)
                     /locus_tag="IWI57_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBC2449984.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3432 a         2303 c         1721 g         4059 t
ORIGIN      
        1 catattttct attattggtc tagacgtatg atggtaaact ttgtacctat ctgattgctg
       61 aatcattaat tgcttatcat tagccatctt tgtttcaagt tcgctagatg atcattttat
      121 tcacatccta acaaacttgt cacatttata gattcttaat ctatttatca tacaattata
      181 aaaaaggagg tgctgcttat gaaagctatc catatttctt tgcttttttt cgctagtgca
      241 ttgattttat ttgctattca tctttgttcc cagccttata tcaataacaa cggaatatta
      301 gtggaaccag cattcttttg cttaccactc ggctttttgt cccttcttgc ctccgcaggc
      361 ttcggatttg ttgctttttt taaacgatca aaacaacaaa tctgacttat aaaatttacg
      421 taaaagagat cgtgacaata atcgtgtgat attcccaaat ttcgaataaa aggtgcccct
      481 tgccatctct aactcctcag tcacaagtta caatattcat aaaccatgaa aagcttgttt
      541 attgaaattg ctccttgtga cttggagcta agtgacagat tacaacctct tttgcatcat
      601 aagactttac tccttgaaaa acattactaa cttttgatat gtttcttgcg cttgtttatt
      661 gctgaaatca aactgatatt catggttcaa aacagcattt ttctccgcat aatctacttt
      721 tgtgactttc acccccgctt tctcaaatac agaagttact tcttccattt gtttcgtaaa
      781 cgtatttgta tttccatccg ttaagaatgt gttttgcgtc caacgatccg catgtctgac
      841 caaggctaaa cttttgacct tctctgattg ttcaaagtcc ttattatcaa agtaagccca
      901 tccacatttt tcataaagaa agctagagat tttatcactt acttgagtaa gtgcagaagg
      961 atccaacaga ccagacaaag aaataaactt cgtaatctgc ttgttctcca aagtcggatc
     1021 aagatgatca gcttttttaa tttcttcatt ttgaagtgct aacaaatatt ctccactaat
     1081 ttgtgcacct gccgaatttc caccaatcga aatattcgac caatctgctt gatcttttat
     1141 ctctttttta gctgcttgaa tggcttcgtt caattggata actggtgtag gatgttttgc
     1201 ttccggtgat cgttcataat ctatatttac gatgatttgc tgcgtgtctg aacatagata
     1261 ttccaaataa tcacgacaat catttttatc ccctcctaca tatgcgcctc catgcatcca
     1321 gaaaagaatc ggtaattttt tcttttccac tttcggtgtg tagatatcca tattattatt
     1381 tttgtacttt gaaggatagg atatttcttt ttttatatcg atttgttcga ttttttgctg
     1441 gaaatcatct ggtcgggcat atacttgttc tttttcactt gtgtcaaatt gttttctaac
     1501 aagcaagcta atcggctttg gtgtgatttg attaatcaaa aatacagtga ctaaaataac
     1561 tacgatgata actggtactg caatcactat tcgcttgaac catttcattc atttaccctc
     1621 tctttcttcg ccaaatccat gtgataatcg tgctaacaga taaaaatcca cctgaataaa
     1681 tgagaagcga atgtttcttt tctccagttt tcggcaacat tctttttttt gcatctgtct
     1741 ttttttgtac tttcactgtc atctgaatcg gttgcgtatc atcacttgat tgatcctctc
     1801 gaggttcatt ctttccctta ttgtctgcat tctctgtcgg atcttgcttt tttggtggag
     1861 ctactgggat ttttttgtaa tagaaaataa tagttggtat cgtctcatta aaaatccctt
     1921 cagtcggtcc ttcaacattt gctaattcgt atccttcgaa tgtcttttgc tctgtcatat
     1981 aattttctcc ataagctccg taaagctgga tttcgtcctg gtctttgagt agacttttat
     2041 tgttttcatc cacatacttt acgtttatct gatcatggaa cactactttc aaaaagtccg
     2101 ttacatgttt cgtgtaggca gcatggtcaa cttcctgtgc ctttccatgc cctgctcctt
     2161 ctactaataa aagatcttta taccctcctg ataactcata taatttttgc gcgttagaca
     2221 caggtactac atcatctgct gttccatgga taatcaattt aggcaatgaa tctgacattc
     2281 caatcgtagg tagagcagct tttgtatcca aatgcatctc ctgatcaaaa tagtattgtt
     2341 tcaaaaggcc cataaataaa tcttcatgct cttgatcccc aatgatgtct aactgatttt
     2401 ttatggcatc cggtagtgcg ggggctactg tctggctgta cgtaaaacgt agttgttcat
     2461 caattgatgc aaatccgcaa ttttcaataa ttcctttgac attgacagga atatctttcg
     2521 ctaatacaga catcatcgta gctgctccca tcgaaccacc ataaagtaaa atcttttgag
     2581 atggccgctc tcgaacttct tgatttatcc agtaaaggac gtcatctgac tcatattgtc
     2641 cataggtcac ataatcgcct ccgctattac cagtcgctct ggaatcaaca aataataaat
     2701 tatacccggc cttataaaaa tcattgtatt cttctgtaac gatcccgtta ttccaattcc
     2761 ctcgaaatcc gccatggatc atgactgttt tatctgaacc ttgatcgaca taatatgcat
     2821 atacatccat cattttgcca tcttgtttgt ttagtacagt ttgatgaagt tgttttgtat
     2881 ttggatagaa gcctgtgttt tctgtaggcg ttgtcccatc atctgtacct ccaagcatcg
     2941 acatcaacac actgtttttt gcaaaagcga tatcgtacaa tagataagta gcaatcagat
     3001 gtttgataat ctctttatct acaccataat cattaacagc aagctcagtg actaataact
     3061 ctattaattt tgacttgatg tcttctgttg gtccattaag tatttctgtc atctgtttac
     3121 cgaagtaatt caaatattct ttaccattga aattttcacc tttaccgata tcatcatttt
     3181 ctgtcccatt ctctttgtca cttactttag cagaagagga ttcgtcgttt gacatttttg
     3241 tagctgttgc ttcttctgtt tgttggttat ctgtggtagc ggtcgtttct tgatctgtgc
     3301 tagtaacaga tgtctcaacc gttgtttcag tatttgtttc tgtttgtgaa gtactcgatt
     3361 tcgtttcatt tccagcatca gtagtttctg tggtttctac ctcccgagat tccgattcgg
     3421 aagaaatgct ctctccatat actgtccatt gcgggacgct tgtacttaca gcaaataaga
     3481 tgaccaacgc tccctttaat acttgttttg ttctcaagct attcactcct ttttatgttt
     3541 ctaggagaat tatatgtaaa cgcaaacatt ttttatacaa aaaataaatg gtttatccca
     3601 taaaaataga acaggattat tttctcattt tacgtaataa agcagaatgg ataaaccagt
     3661 atccattctg ctttttttaa acgaagaaac aacttattgc tccaaaaatg tcagaaatcg
     3721 tttatcccaa attttctcgt aaaaagcgat tgtttccttt gctttcataa aaggattatc
     3781 ataagttgtc gttgctcctc tacacatcat aagctggtat cctaatccat gagcaaactt
     3841 cacagtcgta tctatacaat actctgcttg tgccccacat aattcgatcg aatgacagtc
     3901 caattcagtt aataaaacac tcaaattagt cttataaaat gaattcgcat gggtttttct
     3961 cacaaaataa ctgtgctcag gaatagataa ttccgacaat agctcccatg gttttgtttg
     4021 tggcactaat tgagcatctt cgtgttgaac gaaaataact ggtttcccaa tcgtttcgta
     4081 tgttttgatt cttttattca ctaatgctgt cactgtttct aattcgtgta tcggcgaaga
     4141 atcaaaacat accccttttt gtaaatcgat cactactatt gcgtcggctg tgtttgacat
     4201 acttgtgcct tctttctttt ttcttgattt aatagtattt cttatctagc tagttccatt
     4261 atacgtgaca acattttctt attttacacc tctaaatggc acttttcgac tatttcttca
     4321 tgttattatt tcataaatac ttagctattg gaggaatgta tgatgattaa attgtataca
     4381 ggttctggct gctcgtcttc aagacgcgca aaacgttggc ttatcgtcca tgatataccg
     4441 tttatcgaac agaacttaat tcgtcaacca ctaaccaaaa atgaatttat ccacttacta
     4501 aaattgacaa acaacggcgt gaccgaaatg ctctctactc attctattag ttataaaaat
     4561 cttgttcaca gaatagatga actcagtttg agtgaactat atattgagat tcgtacatcc
     4621 ccttctttat tgaaaaaacc gattatctac gaccaatctg caaaactttt gactggattt
     4681 agcgaagata aaatcaggat gttcctttct aaaactaaaa gaaagcaaga gctcaacgaa
     4741 ctcttgcttt ctggtcatct tgtcgatttt aaagaaaata atctttttgc aatgtcttaa
     4801 ttttggcata aacaatctgc ttttcttaat taacgaaatc caatttttcc aatatggatc
     4861 tatttttctc attcctgatt tttatgattt cagctttcag acgcgcccaa tcattttttg
     4921 taggttgtcc attccagtaa aatatctgtg tattcgtttg atcatatgcc tttttgtcga
     4981 taggataatc gctaatgaca atatcaggta actgatcagc ggatgcacta tattttattg
     5041 ggcgttcagt catagataat atccttttct ttgcccataa ttcctgtgtt ttaccatact
     5101 ttgattgaat atagaatatg acaggttgtt cgtgttttat aaaaatatcc ctgactaaca
     5161 tacagtattg aaaaatcaac cgtctgttct tttcataata agtattaagc tctggaattc
     5221 tgcgagctac cccactaaaa aactgttgca tgattggata catcactgga aatatatgct
     5281 gaaaatctct gtcttctgct gattttccaa aagcatccac cttctttttt gtcccaaaaa
     5341 cccttgaata caaatggatc attgccaaat tgacatatag ataatttttt tctgaagttg
     5401 taaaacaaat cttgaagttt ttttcaaaat tttctacaat agacgattca atccagcccc
     5461 aactttcttg tactttatct ggagtatcca acaaatcttc ttccaaatag gtgttcatta
     5521 caccaaacat aaaataaagg taagcttgct ctgttggagt gatgtgattc atcgaccaga
     5581 agtttgcttc aaaaaaaggc tctagtatgt aatgcgtaaa ctcttctttt gttatcaatg
     5641 gatgcacaaa tttgttcatc tcttctggga catacgtaat cgagtggcct tgtttcattc
     5701 gttgcaacga aattcttagt aggctgatcc atttctttct atctatacta cgcgtatatg
     5761 gcgcatgttt cgcaaatttt tctaatagtt gacgtttatt ttttatactt gataatatat
     5821 cagaatgatt ttcgtcgaat gactgccaat acatcaagtg aaaaaagtat cggatacggt
     5881 attcttctcc ttctatcgga ttaccttttt tcaagctgca agataaatga tagtttttca
     5941 aaatagtatt aagttctctt aattttcttt gaatcgtacg caaacttata taattttttg
     6001 actaagattt tctaaggatt gataagtacc tttgtaaagt gaatccagta aaatataacc
     6061 aatcgttccc tctaaaagta tagaataaac tttactcagt aaaaactgag aagaaaactc
     6121 gacttttatc atcttgtttt ctgaaagaaa agataattta tattctggat acctttcgca
     6181 aaacgattct gcttgttgaa tcaccgaatt catcttagcc agggtgtagt ttagatctaa
     6241 agcgatatcc ttcaccacat ataatttctc gtctctttca taacaatagt tcaaaaattg
     6301 atatacttgc ctttcattga tatcaataaa ttcttcaaac aaagtacata ctccttttta
     6361 tctattattt agacttattc cgacagctac atgaataacg tacaaataga ttcgttactg
     6421 atttttcaga gatactacca tgcgcagtgt gcattgtcaa ttattcttgc tcttattttc
     6481 catttgtgtt cctgttattt cctgtttctc aaatatgtta gtaaacaaaa atagatctgg
     6541 caaagctaca acttcgccag gcaatacagc actctcgaat gaaatattca ctcaaaaatc
     6601 tatcgtttta ttcaatttta gatctgttgc ttttattctt tatttcactc gatggaagag
     6661 agaacgaaaa tagcttgctc ctacaatcaa aatgagtaac acacccatcc atgtaattag
     6721 cttgttcaag gttctactac cagtttctgg taaatcgctt tttctattat ctgtttcttt
     6781 cgctttttct tgatgatcaa ttccacgaga aacaaaatca ggctgtttta gcacactttt
     6841 taggttgctc cctgcatttc tcttttgttc cttagttggt atgataggta ccaaatcaag
     6901 ttcattattt acttttcctg tgctgctttc gctgctcgtg cttcctgtgc tactctcact
     6961 actcatgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     7021 gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc tcgtgttttc
     7081 tgtgctactt tcactgctcg tactctctgt actgctttca ccgctcgtgt tttctgtact
     7141 gctttcacta cttgtgctat ccgtactgct tggcggtcca acaactgtcc aactgcttgg
     7201 tttcaaattc tccgtgatcg tcatctgtcc atcataaaaa acaatcaaat caactttatc
     7261 tttactcttg atctcatcaa tcgatagttc tgcagaaatc attattgtat actcaatacc
     7321 agcggtccat tgtacgtctt ttaaataaac ttccccaata actttttctg aacttccatt
     7381 accatcccaa ccatatgtta gaacagcatg tacatcacta gcattcacgc ttttgtttgg
     7441 tttgactgga atagtaatga cttgttgtgt catgtcataa acaatccgat cagttttcac
     7501 agtaaatgaa agattctcat ttgcccgttc atttgcagaa gtactgattc caatcattgc
     7561 tgcaattata ctcagcccaa gaataacaaa taatacggtc gctgttttct tcatttctat
     7621 cactctccat ataattacct tctatttgat aaaggaatta tataaaatga aaatgatgag
     7681 aacacgacag tcagttgtga aaatctaaca aaagatgtcg tgtattcgct tgaatttgtt
     7741 tactttttcg tttaatcaat gacagactta aaaaagcaag accgatatca gccttgcttt
     7801 cttcatcttt tattgataaa cagcagtgta tttcttcatc cgctttgttt tatatgcata
     7861 gagtaacaaa ccaacaatca agactaacaa gaataatgta gttgatatag atgcttcagc
     7921 cccaaagttt cccccagtaa ttattgtatt ttgcgattgg atcagagtgg agaaaaaacc
     7981 ttgttcttgc gttccgctga ctggtatacc caaaatagct ccttgtgtcc aattccaagt
     8041 aatatgagca ccaatagccg taaataaact tcctgtactg tagataagtg cgctgaaaaa
     8101 gcaacctgct aaaaagatat tgaaaactgc cagaaatgtc atgttaggat tcaatccgtg
     8161 caaaagagca aataatgcag cagatccaat cattgctatt aacggaccaa atctaagcat
     8221 ctgatgctga aaaaagccac ggaataaaag ctcttctgat gcccccactg caataaataa
     8281 aataaaactc gttatcagcg gcacaatgtt tgcgtttcct gaatctaacc gatacgcccc
     8341 cgtcaaatat aatggactag taataagcag aaacactcca attccaacaa cgagacctcc
     8401 cgctaaatac ccccagtctt ttcctcgaac agaaacacca taatcttcta ttctttttcc
     8461 ttcgacctta ctagcaagaa ataaagctag taaaaaaaca gctaaatgtc cgaccgtacc
     8521 ggcaattgct ccaatccatt cactatttcc aataaacata tccgcaagtg cattattgac
     8581 aagaattcct aaaacaaaac caactaagac tattaaaaca gatccaatca accgaataat
     8641 cctgatcatc tttcatccct cctttatatt aagtttaaca acccttttct ttcttgtctc
     8701 gtgaaaacta tgtaaatgtt ctccatcata gagagaatag atttttacga actaagaata
     8761 tctacagcaa ccttacgaaa atgaaagact gtctccctaa ttcttctgtt agactgtttt
     8821 taataaggaa aggatgggaa attttttgaa aaaacaacta ctaattatgt gcctttcagc
     8881 atgtgcaatt ttactattct caggttgtag caagtccaat gaaacgattg caacgtttaa
     8941 aggtggaaca cttacagttc aggattttta tgataaccca aaagttaaat caaataatca
     9001 aacactatta aaaaaaatga ttgtcttcaa agcatttgat gatgtatacg gaaaagagat
     9061 ttcttcaaag gaagtaacaa aagaatataa tgaacaaatc aaaaaactcg gtccaaacta
     9121 caaggaacaa ttaaaagcag ttggccaaac agaagagaca tataagaaac tctttaaaca
     9181 gatgctggct ttccaatacg gtttgaaagc caatgtaaaa ttaacagata aagatctaga
     9241 cactgcttgg aaagaattct atccagaagt tagtacacag atcattctat tttcgactaa
     9301 agaagaagca gataaagcaa aaaaagaagc aaatgaaggg gaaaatttct ccaagcttgt
     9361 tcaagcttat ggaaaaaata aaacattaaa ggaaactgat ggaaaaatga attttgattc
     9421 aaccaatcca gagattccta ctgaagtaaa aaaagcagca tttaaactaa aaaacgggga
     9481 agtttctgat atcattcctg taacagatcc aacgacttat cagcaaagct attatctagt
     9541 aaaaatggta aaaaaacagg ataaaggttc taataaagac aaatacaaat ctgagctaga
     9601 aaaaattgct accgaagcaa aaactcttga tacggaattc atggataaaa caatccgtaa
     9661 agtgatgaaa aaagacaatg taactattaa agatccttat gtaaaaaata tattcaaata
     9721 aacaaatttg acccaattgg ggatgtggca aaatttgaga agtgattttt ggaaattcct
     9781 tcttattttt gaggcaaacc acttttgcca caacttcatc ggggcctttt tttatttgta
     9841 ccaaaacaaa tcactggatt tgttacaatc atgaggatat aatttaaaaa agttttttat
     9901 ttctatcaca tgaatcaatg cgccattaag tagattcttt catgcttccc tattttttca
     9961 cttgccgtat cgcttcaaaa acaactatca taaactcaaa ctcgactgaa aagttatttt
    10021 aaaaaagact aaaaatatcc cttctggact tgttcctctt attctgttat actagagaaa
    10081 cacgaaagat atttttatgc gacacttttc atttgttttt atgaaattta ttcaaaactg
    10141 gcgtaacttc agagttacaa ggatgtatta gaggttaggg aatacatcgt ttaaatgaga
    10201 aataagtaat agtcacccaa actttaaatt aatgaatatt gaatcgtcag atagcctcct
    10261 cgagttttcg agaaggctat cgtttatttt atggagaaat tgattcagaa gtaatcagcg
    10321 acctttttta gttcaccaat cgtttctgct tggaaagttt gcacccaatc ttcttggaag
    10381 cccctcactg ctttatcaat cgagggcatc tctaatactt gatctagtag ctctttacct
    10441 aacgttatcg catgagctcc agatcgaatg gctgtagtga cttgttcaca atttttgaaa
    10501 ctagctgcta atatttttgt tggtctttgc gtacgttcga tctcagtccg caacgaatca
    10561 atcactttca tagcatcaat gccttgattt tgcatcctgt tataataagg tgcaatataa
    10621 tctgctccat tattaatagc taaataccct tgtaattctg tgtaaatagc tgtaccggta
    10681 atcaaataac cttctttttt caactttttg atcgcttgaa ggcctgcttg attcaccggc
    10741 accttgataa acgtatcttt tcccagttct tctatgataa ctcgagcatc ctctatcatt
    10801 tcttcttccg ttcttcctat tgtctgaata tggataggaa tctgtccgat tatttttttg
    10861 atttctttca tatgcgagaa gaagtttgac gctccttctt ttttcagaat tgtaggattt
    10921 gttgttacgc ctgttaaatt caaaaactgt tgacaagaat aaatactttt tgtatttgct
    10981 gtatcaagca tgatttccat atagctttcc cccattctga tcctcttgat cgattttaat
    11041 gattgtcccc tttcttaaat gcggcatttc cttatcttct acatacattg ttcctggaag
    11101 ttccggctca tgagatccat caaattttat cgtgatatgt cctaattcat tcaaattagt
    11161 ttggacaaca tctcctactg ctgtgattac atacatttgt ttatcaaaac agagcttcat
    11221 atcacaagtt atttcttttc ttacctgatt caattcaatc ttataacaat aatctgttaa
    11281 tgtttccggt gcttcatctc caaataaaat cattattttt tctttcctga atacttctgc
    11341 ttctatacca acatctgtta caaccgtttc aaagatcgtc atctctgttc ctcctcgtaa
    11401 aatatcattt gtctaatagt ttttattcaa aatgtttcta gattataaaa tttattgaat
    11461 aacacattcc taacggaatt tgtttcgata ttgtgtgatt tctcagagta caatg
//
LOCUS       DADXTG010000080        11514 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.081, whole genome shotgun sequence.
ACCESSION   DADXTG010000080 DADXTG010000000
VERSION     DADXTG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.081"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(79..2154)
                     /locus_tag="IWI57_001846"
     CDS             complement(79..2154)
                     /locus_tag="IWI57_001846"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBC2449985.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IWI57_001847"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IWI57_001847"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBC2449986.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IWI57_001848"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IWI57_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBC2449987.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="IWI57_001849"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="IWI57_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBC2449988.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="IWI57_001850"
     CDS             complement(5326..5721)
                     /locus_tag="IWI57_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBC2449989.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IWI57_001851"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IWI57_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBC2449990.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="IWI57_001852"
     CDS             complement(6198..8390)
                     /locus_tag="IWI57_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBC2449991.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="IWI57_001853"
     CDS             complement(8414..9385)
                     /locus_tag="IWI57_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBC2449992.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="IWI57_001854"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="IWI57_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBC2449993.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3111 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaa
//
LOCUS       DADXTG010000081        11345 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.082, whole genome shotgun sequence.
ACCESSION   DADXTG010000081 DADXTG010000000
VERSION     DADXTG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.082"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(180..1028)
                     /locus_tag="IWI57_001855"
     CDS             complement(180..1028)
                     /locus_tag="IWI57_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBC2449994.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1027..2094
                     /gene="gnd"
                     /locus_tag="IWI57_001856"
     CDS             1027..2094
                     /gene="gnd"
                     /locus_tag="IWI57_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBC2449995.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2096..3097
                     /locus_tag="IWI57_001857"
     CDS             2096..3097
                     /locus_tag="IWI57_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBC2449996.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3094..3378
                     /locus_tag="IWI57_001858"
     CDS             3094..3378
                     /locus_tag="IWI57_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBC2449997.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3411..4208
                     /locus_tag="IWI57_001859"
     CDS             3411..4208
                     /locus_tag="IWI57_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBC2449998.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4222..5055
                     /locus_tag="IWI57_001860"
     CDS             4222..5055
                     /locus_tag="IWI57_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2449999.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5073..5930
                     /locus_tag="IWI57_001861"
     CDS             5073..5930
                     /locus_tag="IWI57_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2450000.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5952..6461
                     /locus_tag="IWI57_001862"
     CDS             5952..6461
                     /locus_tag="IWI57_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2450001.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6498..6899
                     /locus_tag="IWI57_001863"
     CDS             6498..6899
                     /locus_tag="IWI57_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450002.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7011..7364
                     /locus_tag="IWI57_001864"
     CDS             7011..7364
                     /locus_tag="IWI57_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450003.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7398..7841
                     /locus_tag="IWI57_001865"
     CDS             7398..7841
                     /locus_tag="IWI57_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBC2450004.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7858..8943
                     /gene="uxuA"
                     /locus_tag="IWI57_001866"
     CDS             7858..8943
                     /gene="uxuA"
                     /locus_tag="IWI57_001866"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBC2450005.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8936..9649
                     /locus_tag="IWI57_001867"
     CDS             8936..9649
                     /locus_tag="IWI57_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2450006.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            9978..11279
                     /locus_tag="IWI57_001868"
     CDS             9978..11279
                     /locus_tag="IWI57_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2450007.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3763 a         1864 c         2451 g         3267 t
ORIGIN      
        1 atctaacaag taactcgaat aataccaata caaccttctc caaagatgcg atgacatacg
       61 ttctaaccca ccgactattg atgttctgta ccacccttgc agaaccaaaa aagccactac
      121 caaatatttg gtagtggctt tttttgtttt atggattttt cgctttttct gttatggtgc
      181 tattctttga tttcagaaat agtatgtcga actttagcga tagaacgacg tgattctttt
      241 tcgttgtaaa acatcaaaat cacatataga gagtccaaaa tgcttagttg agaaatacgg
      301 gaagctaaag cttcagatcg gaattctgtt tcttcagaaa tagaaataaa aacgacatcg
      361 ccaagtttag caagggaaga attagcttgg ctagtaataa cgattgtttt tgctccagtt
      421 tttcttacgg tttcagcaat atggattgtt tcttttgatt gccctgtgtg agaaatcaaa
      481 acagcacagt ctttttccgt caataaagaa gcttccatca attgaatatg gtaatccgtg
      541 ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat aagcgtcagt tgctacaatc
      601 tctgaaccgc caacaccgaa aaaatagaca cgtttagatt cattgataat agtagccgct
      661 ttttttagat ccttttgttg taacaacttt tttgtgtcat ttaaagttga aatactagac
      721 tcgaacacct tttgcgccat agtgagctca gtatctgttt tctgaatatt ttcgtggata
      781 gaaatagcag atagatcatt ttcttggatc aacatggcca ttttaaaatc tttaaatcca
      841 ttataaccaa gtttttttgt aaattgaaaa aaagtagagt cagcaatgcc cagttcactt
      901 gctaaattgc taataggact atgacttact ttgcttggat tttctaaaat ataatcggcg
      961 atagcctgtt cttttgggct gaagtcgttg tacattgttt tgatttgtag tcttatggag
     1021 acgctcatga gaattcctca ttttcataat aagattatca ttttttccgt tctcattaat
     1081 gttaaagata caaggagagt aggaaaaaat caaacaaaaa atatttttgt aaaatctatt
     1141 tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat catggaaatt
     1201 ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa agagcaccat
     1261 cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag agaaaatgac
     1321 attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa aaaaatcatc
     1381 tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact ttttccaaaa
     1441 ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga ttcaatccgt
     1501 cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac ttctggagga
     1561 atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga aacttttcta
     1621 tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta tgctggtgca
     1681 tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg aatgatgcaa
     1741 gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga cttggaacaa
     1801 gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga aatcatggaa
     1861 gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc tgcatcaggt
     1921 gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc agtaattgca
     1981 actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc aaaagtagtt
     2041 tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga gtaaaatgcc
     2101 agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc cacaccactt
     2161 ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag agattccagg
     2221 aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg ttacgccatt
     2281 ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc gacatggtat
     2341 tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg tatctatcat
     2401 acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt tgttgtccat
     2461 gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg aagatcgggc
     2521 ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg caggaaatac
     2581 cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc tagcttatga
     2641 tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag aacttcaaga
     2701 attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag agaactatca
     2761 catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat ctaatagtgc
     2821 aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg gaaagatcgc
     2881 aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc cgtatttagc
     2941 gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat gtttagaagc
     3001 aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc ttcctgaacg
     3061 ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt gaacggttta
     3121 aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc aaaagtttct
     3181 gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac ggtatcatgg
     3241 gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt tttttatcat
     3301 cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat catcaaaaaa
     3361 atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta gtgggttata
     3421 gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt aattgtgctc
     3481 catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg aaaacagaac
     3541 acttttacag acaagctgct ttttttataa cagatcccca attagtgatt gattcccctg
     3601 ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa tctgagctca
     3661 tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa gatactgcga
     3721 agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat atggcacaaa
     3781 gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt gctggtttag
     3841 ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg catgacgcta
     3901 tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta gcgtatggga
     3961 ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta tatcctttgt
     4021 atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg aaagataaac
     4081 agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat ttgattccta
     4141 tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt tatatcagaa
     4201 agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt gttgattgct
     4261 ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt gaccttacgt
     4321 ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt tgcacaagcg
     4381 atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc aggaggatca
     4441 atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt attaggggat
     4501 ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg aagttacttg
     4561 tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa agctgtagaa
     4621 gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt tgcctcattt
     4681 ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt gatcgctgat
     4741 gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt aggtggaggg
     4801 ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga ttatctggtc
     4861 tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat tacgatggtt
     4921 acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca aaaagggaaa
     4981 caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga agatgactat
     5041 gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat aatcaaacag
     5101 gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga tggcattttg
     5161 caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta tacggaatga
     5221 tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca tataaacggc
     5281 agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact ggcctaatgt
     5341 cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc ttgatgcagg
     5401 atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga atcggagatg
     5461 caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct tgggcacaac
     5521 agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat caggttttac
     5581 taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca ggcctaaagc
     5641 aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg tttatgatgg
     5701 gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg atatccggac
     5761 gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta cctttagctg
     5821 tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca caagcattat
     5881 tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa aaagaaggga
     5941 acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg atccatgggc
     6001 aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt gtagacgaag
     6061 ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc cgtacaggac
     6121 aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa tatcaattta
     6181 aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac ttagtaaaac
     6241 acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat cctgatacag
     6301 atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa ttgaaagaaa
     6361 tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct gcacctaaaa
     6421 caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct tagaaaaaag
     6481 ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa tttcgttatc
     6541 gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt tcagcattag
     6601 tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat acgctagcct
     6661 accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt atctgtactt
     6721 attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg aaagaccaaa
     6781 ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt ttaggttggt
     6841 ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta tccgtataaa
     6901 caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa gcagctcgcg
     6961 gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg atgctagaaa
     7021 tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa ggctgttatg
     7081 gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt cttttttatc
     7141 tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt tcaaataaag
     7201 aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat atttttagtg
     7261 caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga gggaaaacat
     7321 ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa agtgcaaaac
     7381 aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag ttgagcgaag
     7441 gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa acaatgtgct
     7501 tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa ttaactatga
     7561 tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt ccgtacaatc
     7621 aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact tatgtcatag
     7681 ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt cacacaccga
     7741 tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact gtttggcatg
     7801 cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc tgaaaagatg
     7861 aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa atatgttcgc
     7921 caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc tggagatgtt
     7981 tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg actggaactg
     8041 cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga tgaacgagat
     8101 tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg ggttcgattg
     8161 atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt taatgaaaac
     8221 aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat ggagccagga
     8281 gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg ttgggaagaa
     8341 gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac cgaagaagaa
     8401 ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga aaaagcagat
     8461 gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt tcctagagtg
     8521 acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc atataatgga
     8581 ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt tgaaatcatc
     8641 catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt tttaggagaa
     8701 agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat gtatcatatt
     8761 atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca tggtagaacc
     8821 gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat gggactcact
     8881 tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga gcattatgaa
     8941 taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg gacgtttagc
     9001 ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta aggaagtcta
     9061 ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca ttttgtacgg
     9121 agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag aaaaaatcgt
     9181 gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat cttttgcaaa
     9241 agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag tagaaatgta
     9301 tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg atgatgtaaa
     9361 aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga atattcctcc
     9421 aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa ggcgagcggg
     9481 cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata atgggaagca
     9541 taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg aattttatga
     9601 cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat aaagaggact
     9661 attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg aaaaagaagt
     9721 aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac aaacacaaaa
     9781 cgcatctact gttatctacc agtagatgcg tttaaagaga agaacgagaa tcgtccatat
     9841 gagggtttcc acggctctat aatttctagg actgatagaa aatcattcta tcggtccctt
     9901 ttgagcatga aaaaacaaaa catacagaag ctccgttaga attgaatcac cacaacacaa
     9961 tcaaaggagc gttctgtatg cctgatcttc atcatatcaa actactactt ggaatcaaag
    10021 ataaaaacat tttcatcaca aatgtggaat cgaagagcat taagaatatc aagagtcttg
    10081 ttgtttctgc gacgttaagt aaagaaattc gtcgctgtcc tttgtgtaaa cagatgaatc
    10141 atgaaggcat gatcgttaag aacggaaaga aaaaatcgct cattcaactt aataaatgtg
    10201 cgaaccaact gacttatctt gctttagcga aacaacgcta ccactgtcgt ggctgccaca
    10261 cctattttac ggcgaatacc tacattgtcg atagaaactg ctttatcgct aaacaagtgc
    10321 gctataaaat tttagaagag ctaactgaaa aacaagcaat gacgacaatt gctaagcatt
    10381 gcggcgtttc ttggtcgacc gtttctcgga cactcgcttc tctgatcccc atgacaaaag
    10441 taaaaagaaa ttggttaccg cgttgtttat taatggatga atttcgttca ctgaaaaatc
    10501 aagtcggtcc ctactccttt tcttgtatgg atggcgatac tggaaaatta ctggatattc
    10561 ttccttcacg gaagaaaaag gctctcgttt cttattttat gcaatttgaa cgacgcgcac
    10621 gattgaaggt aaaggtcatc gtgacggaca tgaatgtttc ttatgcctct ctgataaagg
    10681 aatgttttcc aaaggcaaaa ctcgttgtgg atcgttttca cattgtcaaa catctcattc
    10741 gtaaatttga ggatattcgc gtacgaatca tgaaaagctt tgatcgaaac gatccaacac
    10801 aagccaaata ttatcgacaa ttaaaggcgt tgtctcggct tctgatcaaa cgacaggaca
    10861 agcttgttta tgacaaatgg accaaatggc gcaattttgg ttgggcgtat ctcaccgaaa
    10921 gtgaagtggt ggatcgtctg ctttcgatct ctgacgaatt acgaatcgcc tatttttact
    10981 atcaagaaat tcttcaagcg tttcacgaca aagaggcgga cactttcttt aaacttgtaa
    11041 gaacgatgcc caattccgtt ccaaaagaac tgcattctat aaaaaaagct tttataaact
    11101 atgaatccgg cattcgttta gccctcgaat tgccttattc aaatgccaaa atagaaaatc
    11161 tgcataccca catcaaagca ttgaaacgag tcgcttatgg ctttcgaagc tttcggaaaa
    11221 tgaagaccag aattttctta ctaaataatc tgattaccta tgaatcaaaa aacatctaaa
    11281 gtagaaaagg aattctacct tagatgttct tatctcactc tatcagtcct atttgacaaa
    11341 gagcc
//
LOCUS       DADXTG010000082        11170 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.083, whole genome shotgun sequence.
ACCESSION   DADXTG010000082 DADXTG010000000
VERSION     DADXTG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.083"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(598..942)
                     /locus_tag="IWI57_001869"
     CDS             complement(598..942)
                     /locus_tag="IWI57_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450008.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1039..1284)
                     /locus_tag="IWI57_001870"
     CDS             complement(1039..1284)
                     /locus_tag="IWI57_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450009.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1354..1608)
                     /locus_tag="IWI57_001871"
     CDS             complement(1354..1608)
                     /locus_tag="IWI57_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450010.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1772..2095)
                     /locus_tag="IWI57_001872"
     CDS             complement(1772..2095)
                     /locus_tag="IWI57_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450011.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2282..2355
                     /locus_tag="IWI57_001873"
     tRNA            2282..2355
                     /locus_tag="IWI57_001873"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Ile,seq:cat)
     gene            complement(2740..3237)
                     /locus_tag="IWI57_001874"
     CDS             complement(2740..3237)
                     /locus_tag="IWI57_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBC2450012.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLFPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3277..4086)
                     /locus_tag="IWI57_001875"
     CDS             complement(3277..4086)
                     /locus_tag="IWI57_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2450013.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4420..5277
                     /locus_tag="IWI57_001876"
     CDS             4420..5277
                     /locus_tag="IWI57_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBC2450014.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5270..6172
                     /locus_tag="IWI57_001877"
     CDS             5270..6172
                     /locus_tag="IWI57_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBC2450015.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6205..6555
                     /locus_tag="IWI57_001878"
     CDS             6205..6555
                     /locus_tag="IWI57_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBC2450016.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6604..7527
                     /locus_tag="IWI57_001879"
     CDS             6604..7527
                     /locus_tag="IWI57_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBC2450017.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7605..8498
                     /locus_tag="IWI57_001880"
     CDS             7605..8498
                     /locus_tag="IWI57_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2450018.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8536..9099
                     /gene="amaP"
                     /locus_tag="IWI57_001881"
     CDS             8536..9099
                     /gene="amaP"
                     /locus_tag="IWI57_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBC2450019.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9113..9301
                     /locus_tag="IWI57_001882"
     CDS             9113..9301
                     /locus_tag="IWI57_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBC2450020.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9314..10216
                     /locus_tag="IWI57_001883"
     CDS             9314..10216
                     /locus_tag="IWI57_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBC2450021.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10248..10490
                     /locus_tag="IWI57_001884"
     CDS             10248..10490
                     /locus_tag="IWI57_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBC2450022.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10746..11117)
                     /locus_tag="IWI57_001885"
     CDS             complement(10746..11117)
                     /locus_tag="IWI57_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450023.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3955 a         1918 c         2121 g         3176 t
ORIGIN      
        1 gttataagct tttttaaaga atatttagaa attattaatt ttcgtctgaa aacaaaaaaa
       61 gaaagcgtta ttattttagt ctattttcag ggggggaacc ttatgataaa aaaactattt
      121 ttactatcaa tattaacgct agcaatggca ggttgttctg actctggctt agcaactgaa
      181 ctgatcacaa aatcaactga agccacgaca gaggagttaa aatgtggaaa cgatcacagt
      241 gactacagag gcttaaatcg acgatgcatg ggaatattag gcagaagcaa ttgtaagcat
      301 cgatgtagta atcgaagaag gtgtagaaga agcaacagaa aaatcaaaag aaagatcaaa
      361 agaagcaatc tgtgctgtgc atcgtataca gacgtcgaag atgcggtact tttaaaccac
      421 gtcaggagat gttcatttat gtctagggaa gttggcgatg aaccaaaact agagtacgct
      481 tttagttttg cacgtgagga tgaagataac cctaaaactt acgacatttt agcttgatct
      541 aagatggtta aagcttttta atgaagtaag aggagataga aggcttttct agttatatta
      601 ggtaagataa attccttttt gctccaaatt tgctaaacat tctgctagat aaacttcatc
      661 gtgttccgga taaatcggca cgttgcgctc tacagtaggt aaatcaaaaa atgcttcgca
      721 tgatttgcca cgatacatag gatttttcca ctctggtgct gggctgtaac cacgtttttc
      781 catttcatcc atgacaagct gatgatattg gaataatcta taaggagaat agtgaaaaac
      841 ataatccaca gtagcatgtt tctttcccca gccatttcct cttaatgcac agcattcccg
      901 atgttgtcct aaaagctgtt gacgaggaag ctttgaaatc aatttttcat gccataatca
      961 catagaaatg tctcctttat atatagattg ttaaccttag acgttaaatg aaaaaaccag
     1021 caccaaggct ggaaaaaatc acttcagttt gcttgctgtt tctgagcgac caattaggta
     1081 atcaacggaa atatcgtaaa agtcagcgat atagattaat tcaatgatgc ttggaatttc
     1141 ttctccttct tcgtacttac gatatttgaa taaaggaata cttaacgcgc tagaaacagc
     1201 tacttgagat aaagatcggc cagttcttaa gaaattcaga cgacaacgta aagtatccag
     1261 taaaatagct tcttcgatac tcatttaatc acctttcatt atagtagaat agttataatg
     1321 actatagaaa gctacacatg caatcaatag tactcaatgt tttttattta aacgtatcaa
     1381 ataaagaatg acgatcaata aagacaaaac cagtcctgat aattcttcta taaaaacact
     1441 atcgggttca aagtagttgt aattaaaaac tttccacata aagtagatag ccggaagagt
     1501 caaaatagct aaataaaacc gttctttgtt ccaattcata agataaacaa tacttgcgac
     1561 cagtgcgacc gcaggcaccc aaaacaaaac tctcaccaac gtaatcatgt aacggactcc
     1621 tcaacgattc atataataaa agataacatg ttattgcata catcacaaat gaaatgctag
     1681 gaaaataaaa gaagaataga tcaatgaaaa atgaaaaagc agaacagttt tctgctgata
     1741 agcaggaact gttccgctga atgtgtctta tctatcgaga aaaaatcaat ggattgatat
     1801 ctaagtaatt caatacatat aaaaagagga cgatcgcatt cactaaaaag gaaagaatga
     1861 tgatcccata gcgaatggaa acatatttaa caaatacttt tgtttgaata cgggcatatc
     1921 tttttccttt atagctgagc atcatgacaa gaagcaaaag ggtaatcaca ccttgaaaaa
     1981 tcattccaat aaacagaatt cctgaggttt gataatttaa ttggagaaaa taagctgctg
     2041 ccgcaactaa atcgaataaa atcgcaataa atggcaaaat aatcactcct aacattgaga
     2101 ataaataaaa gaataacatt ttttctaaaa taagcaaata aaaattaaag taacaaaata
     2161 gttatgacac aagtaaaaga aataagaata acaatattat tttacttata ttttgaaact
     2221 tgtgagagtt tgtcattcgt gctatagttg aaaagtaaca aaaataaatt ttatttatga
     2281 tgtaccttta gctcagttgg ttagagcaga cggctcataa ccgtccggtc gtaggttcga
     2341 gtcctacaag gtacataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     2401 ccaagtaaaa acagcttctt atgtttttac ttggctgttc ttattactgc agatgcaagc
     2461 ttttgaatac cgtttaagga agaggttgtg aaatcagcgt tttgacctga gtattagctt
     2521 aacaaacgga aaaatagttc ctcatatttt gccgtttgtt aagctaatac cgagggtcag
     2581 ctgattgaac accatttaag aaagaggtcg tgagcctgcc gtttcactct gtgtcataaa
     2641 aaagccaatc tactttgata gaaaaagcac gtaggactaa aacagttctg cgtgcttttt
     2701 ttattgtgtg ttaaatatcg taaatgaaga tagatatttt tatgattcga cgatagagac
     2761 gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt tttctactgg agactaacat
     2821 tggatcaaca aaaaagtctg cttcaggaaa agcactctgc aggcctatca gattagagag
     2881 tacacacaag tttgtttcaa tcccgactac ttcgatctct ttttgttctt ctttgctttc
     2941 aaataaagtt tcctggattt cagctaaagt ttcaggtgga atcgtaaagt aatacttttt
     3001 aatcactcgt tcattttctt gtggaaaaag aagagggatc aattgcaaat cttctcgttc
     3061 ttcttctttg tccttcagtt caactggtat atcccgtgtg aagagaatat attcattatt
     3121 ttggcgtgct ttagctaatc tttgagcaat acgttctgcc aattgatctg actctggaat
     3181 ataaaattcg ctgtttggat caagaatatg attttgcata tcaataataa ctaacatttt
     3241 ctttcctcct acattcatat cgtcatttta ttctttttaa tagaagtttt ttaaaatata
     3301 agcaacacca gcttcttcat tcgtcagtgt tacttggttt gcaatttctt ttacttgtcg
     3361 ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc aacatatcaa tgtcgttttc
     3421 ttgatcgcca aaagccatga tgtcactcga ttcgattcca aggtaatccg ccagaaaaac
     3481 aagtgaatga gctttagtga tacctgtagg cgtgatttct aagaaatgcg gatcagagaa
     3541 tactgattgg ataccatctg cttgcagatc tctttgtagc ttctgtagtt tttcttcact
     3601 cttatctgtt aatgctaatt taagatagct aagagaagga tcttccaaca tatttaaaat
     3661 ttgatcaaaa gaatggttct gtaaattgaa atgttcataa taagggtcat ctttgactag
     3721 atttgattca tcgaatgtca gctgatcatc ttgggtttct cggctagcaa tgatggatat
     3781 tttcgcttct tgagcataga agaaggcttt tctcagtgta tcgctatcta tttttgaacg
     3841 tctgagaact ttcgccattt ttccatcctt catttcagca atcagtgaac cattggaaga
     3901 aatcaaatag ccaccgattc ctaattcgga aaaaatatct ttagcagaaa tgctgttcct
     3961 tccggtagcg acggttacga ttccgccatt ttcatcaaat ttccgaagtg cttggatatt
     4021 ttcttctggt atctttccat ttcgatcaag caaggtaccg tctaaatcaa tcgctgctag
     4081 tttcatttta tcttcctact ttctttttac atatctattt tcctgttacc ttaagattaa
     4141 atgagaattt aaaaaaatga aactaacttg ctttgtcatt atgaattttt tcaaaaaaca
     4201 ttcttaagaa aaaggtggct atatatagta gtttttggtc aatacctttg aataaaaagc
     4261 catgaaaaac gaatagttag cgcttaccga tgcgttttgt gataaaaaaa ttttaaaata
     4321 ttcaatgaaa acaaagcata tattttgctt taaaaccaaa tgaggaatat tctagatgta
     4381 gaacaggtta gaaaaagatt agaaaaagga gtgattttga tggagaagag atacggcgta
     4441 gttcctgtgg taactgcttc agacgaaaat tatgctcctt acttgagcgt gatgatcgca
     4501 actgcattag aaaattgcaa taaaacaaga cgcataaaat tttatgtgat tgacgatggt
     4561 ctatcagaat atagtaaaga aggattggaa gaaacggtaa ataaatattc aagtaatgca
     4621 agtatccaat tcctgacagt ggaaaaagat atctacgaag acttcctagt cagtgaccat
     4681 atcacaacta ccgcttactt aagaatttct ttaccaaact tattagctaa agaggattat
     4741 aagaaagtcc tttacttaga ttcagatgtg ttagtactag acgatatcgt aaaactatat
     4801 gatgagccgt taaatggaaa aacaatcggc gctattatcg atccaggcca agtgaaagct
     4861 ttggaaagat taggaatcga ttccgatgac ttgtacttca attcaggagt gatggtgatt
     4921 gacattgatc aatggaataa aaaagaaatt acagaaaaaa ctatccatta cctcagtgaa
     4981 aatggcgatc gaatcatcta tcacgaccaa gatgctttga atgctgtact ttatgaagat
     5041 tgggaacaac ttcatccaaa atggaacatg cagacttcgc tgatttttga acgtcatcca
     5101 gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa atgaaaaacc ttcgatcgtt
     5161 cactttacag gacatgataa accttggaat acgttgaaag atcatccgta taccaattta
     5221 tacttaaaaa aattagcaca cagtactcta acgaaagtag gcgagatcaa tgaataaaaa
     5281 agaaatagca gtagtcgcaa gttgtaacac aaaattcgta ccacatttag ctgcattatt
     5341 tgtctcagta ttagataatt gcaacccgtc taaatttgtt cggttctatg tgattgacga
     5401 tgatatcgat tttgaaagta aacagctttt acgcttctca gtgaaaaacg cacgaatgaa
     5461 ctcagatgtg gaattcttga aaatcaataa agaattcttt acaaatgtgg taatcagcga
     5521 tcgtattcca gaaacagctt attatcgtat tgcgattccg gaattgttcc gcggcacaga
     5581 agttgaacgt atcttatata tggattgcga catgatcgct ttacaagata tctctaaatt
     5641 atggcgtctt gacttcggtg attcaatcgt agcagctgtt gaagacgctg ggttccatca
     5701 acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt tatttcaact caggtttgat
     5761 gctgatcaat gtgaaaaaat ggttagatga aaatattacg caaaaagtat tagatttcat
     5821 cgaacataat ccagaaaaat tacgattcca tgaccaagat gcattaaatg ctatcttaca
     5881 tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa ggctatatca tggcaaaagc
     5941 aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa gaaactcgca acaatcctta
     6001 cattatccat ttttcaggac atgtgaaacc gtggagcaag gattttgaag ggccaacgaa
     6061 gaaatattac gaaaaatatg cagggatgac ggctttccgt tgtgtagcta aattccctaa
     6121 atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta gttgttaaat aatagaagac
     6181 aacaactgaa gagggggagt aggaatggaa attacaatgt ctaaaaatgc agtagagacg
     6241 ttaattgaaa aggttgggga aaatacaaaa atcgcactcg cgcttatcaa tgattccgat
     6301 ccatttctaa gggacaaagg tgcttttgca aaaggcagct tttttcaaat cattcctttt
     6361 gtttcggaat ttggagaata tgcgactaaa atcgagcatc cattattgga catctataca
     6421 tctaaattag agcaaaatta tttcggtaaa cgattgaaca tggacttcaa caaacagctt
     6481 gacagctttt cattagaaaa tgaaatcgca gtactcgatt ataatatcaa attaaagaac
     6541 tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga ggtatacatt gagatactac
     6601 tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa aaaaatataa ttggctcgta
     6661 cttgatagtg caaagcccga agagattgag caagcaatcg aagaattcga gcttcctgat
     6721 gatattttcg taggaaccga atatccggaa gaagtttctc ggatggagcg cctttatgag
     6781 acgaaactag agcatccatt ttctttggta gtgattaatc tagatcagtc agataatcgt
     6841 atcgaaaaaa aattaacgcc aatatctttt gtactttccg ataatctctt gattacttgt
     6901 atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa agcatggaaa tcatttaaac
     6961 tcgtttgaaa agatcatcac gtatacatta ttagatattt acacccatta cgtgaaagaa
     7021 ctaagagaga tgaaaaagcg aattgacgct ttggataaag cagctcgtaa aacaacggaa
     7081 aatgaagaac tgtataaaca ggcagatctt gaaagagaca tcgtatatat cgatcatacc
     7141 ttaagagatc aacgggaaac aatggatcaa ttatggaatt ctgaaacttt catcaaacgt
     7201 ttagacgatg aacagctact ctatgatgtc catttgagac agcgacaaac agaaaaaatg
     7261 atcgagatct atcgagattt attagagagt attggtgatc tattcaacgg gatgatggac
     7321 aataatctaa atcatatcat gaaatatcta gattcagctg cattaatcat ttccgttccg
     7381 gcgttgttcg cagggatttg gggaatgaat acaggaggat taccgggtaa aagttctgct
     7441 ctaggcttta tactcgtagt ctttggctct ttgatagcag cagtagtttt aggttaccat
     7501 ttataccgga aagattatac aaattaaaga atctatttaa aacaggtatc aaacaaagaa
     7561 atttgttttg gcaatacaat ttaaaaacta ggaggaatac agcaatgaca gaaccaaaat
     7621 taatcgaccc gcgaaacttg taccatacgg agaaatttcc taaacaagat caggatacac
     7681 cagctttaca gcagaatatg attcctgttc ctgattgtgg agaagaatcc tatgaaggca
     7741 ataatcaact agaaaaccgc cgtgtactaa tcactggtgg agattcaggg atcggtcgtg
     7801 cagcagcgat tgcatttgct agagaagggg cagatatcgc tcttcaattc ttcccaggag
     7861 aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaagt tgcaggacgt aagattgtct
     7921 tactaccttt tgatcttcgt gacgaacaag cacctaaaga aatcatagat aaagccgtga
     7981 gtgaacttgg cgggctagat acgatggtac taaacgcagg acagcaaatt tcttgtcagt
     8041 ctattgctga gttaccactt gagcaagtca aagacacatt tatgataaac atcatcgcta
     8101 tgtttgcttt agtgaaagaa gctgttccgc atatcccagc aggaggagca atcgtaacaa
     8161 caacttctgt tcaagcattc aatccaagcg aacatttatt agattacgca gcaacaaaag
     8221 cttcaatcgc taactttacg atcggtttag caaaacagct tgcacctaaa ggaatccgtg
     8281 taaatggtgt agcacctggt ccaatctgga caccattaca actagacgaa ggacaacctt
     8341 ctgattcgat ccctgaattt ggtcagcaaa gtttactaga acgtgcaggt cagccaggag
     8401 aattagcacc agtttatgtt ttcttagcat cgaataaagc aagttatgtt acagcccaaa
     8461 tttacggagt aactggtgga gaagcgatca acttataaga agttgatcgt ttcaccccta
     8521 tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg gagtgatcgg aacattactt
     8581 ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa cctatccatt gccttttcag
     8641 ctagagaagt ttcgattctt ttccatcact aattcatacg tgcaacaata tgtattttgg
     8701 cttgccgttg catttgctat tttgacaatt atcacgttac ttgttttcct gttttatcca
     8761 aaaacaatcg gaaactttct actgaaacat ggtgatgggg agttgacgtt ggataaaaaa
     8821 gcaattgaag gaatggttcg ttctcacttg catgaagatg aattcatcca ttcaccaaaa
     8881 gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg taaaagggga gttgaaacga
     8941 acttcagcac tcattggtaa aacaggaaca ttgatgaaag aaatcgaaaa tgaagtacaa
     9001 cacatattgg gaacaaaaga gccagtgaaa gtatcggtga aatacacagg ctttgaagaa
     9061 ccagctaaac gaagcaatga ccgcaaacga gtagaatagg gggggaccaa aaatgaaaga
     9121 actattttcg acatacaagc tccctatcat ctttggggta cttggattga tattagccgt
     9181 tttatggatc accgtgggct ttttcaagac cttattgatc ttgatcatgg cagcgatcgg
     9241 cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta gatcactatt ttaaatctta
     9301 ggaggaaaca tccatgaata acaataacaa aaataataac aataaaatga acaaccaaaa
     9361 accagtgtct gcacatccag acccaacgac accaaaccaa tcaaatggtg tgaacgatga
     9421 aaagaacaaa acagcaccag tatctgcaca tccagatcca gtagtcccta acgttcaaca
     9481 aggagtagaa gagaagaaac atgatgcaga aaagcatgca cctcatcatc caggaactcc
     9541 agaatctaaa gttccagaac aacatgatgc aatcgatcct tctaaacctg tggaagagca
     9601 aaaacatcct tctgaccatg cgaaagcaca agaaaaactt agttctgacc acacaaacgg
     9661 acaaggaaaa cttggttctg atcatacaaa taaccaagga aagcatgatt ctgaacagga
     9721 agattcaatg cgcggtgagc tgaattatga agataaagtt gtgcaaaaaa tcatcggaat
     9781 cgcaattgaa caagtagacg gtttattatc tgcaaacggt ggtttcttct ctaatgtggc
     9841 aggaaaactt gtgaatacag ataatgtaac ggctgggatc gaaacagaag tcggtaaaaa
     9901 acaagtagca gttgatatgg atgtcattgt ggaatatggc aaagatatcg aaaaaatctt
     9961 tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat atgacacatt tagaagtgat
    10021 cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa gaatttgaaa aagatcaaga
    10081 aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc ggtgaatttg cttcgaatca
    10141 aacagataaa gtaaaatcag cagctggaag cggcgcacaa aaagtaaaag aaaatacaga
    10201 accacgggtt cagtaataag gaagtaaaac attaggagga acgaaacatg ggatttattt
    10261 ggtcacttat cgtcggagga attatcggag caattgcagg agcaatcacg aataaaggtt
    10321 cttcgatggg gattattgca aatgtcgtag caggtttagt tggatcagca cttggtcaag
    10381 cattattagg ttcttgggga ccaagtattg caggaatggc aatcgtgcca tcattgattg
    10441 gtgccatcat tttagtacta atcgtatcat tctttgctcg aaaactgtaa aaagactttt
    10501 aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata agaacagcca aacaaaaata
    10561 gcttctcata tttttcattt ggttgggcat attatcgaag atgcaagctt ttgaacactg
    10621 tttatacgga ataagaggct gtgacaagac ttttgtcaca gcctcttatt tatgcgggca
    10681 aattcccaat tagattttta aaagtttttc cttaaatgtg tctttattga tacagagaag
    10741 cttctttaat aaaacaatgc ctccctaaat tttcttgaga taggaaaggt gaacatacaa
    10801 aataatacga ccactttgaa aacccagatg atccacaaat ggatcgtatg atactgatga
    10861 agtaaaaagc aatggatcag aaactcaaag agattgaaaa agacaaggaa gaaataatat
    10921 ctctttttta ctcttgtctg ttttagccaa taatagctcg taaagtaatc tagacaaaca
    10981 aatagaagta aacaaagttt cgtaagagca gcaatcgtat catgttgctc tgctagacga
    11041 ttgacgagca ggaaaaatgt tggtaatgga atatagatca cgagtaatga aagaaaaaag
    11101 taaacaataa atggcatact attcctccta attgtgtgag ttcgccctca ttttaaaata
    11161 acgatatttg
//
LOCUS       DADXTG010000083        11129 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.084, whole genome shotgun sequence.
ACCESSION   DADXTG010000083 DADXTG010000000
VERSION     DADXTG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.084"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWI57_001886"
     CDS             6..305
                     /locus_tag="IWI57_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2450024.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWI57_001887"
     CDS             378..1739
                     /locus_tag="IWI57_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBC2450025.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWI57_001888"
     CDS             1761..2021
                     /locus_tag="IWI57_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450026.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWI57_001889"
     CDS             2035..2787
                     /locus_tag="IWI57_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBC2450027.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWI57_001890"
     CDS             complement(2881..3486)
                     /locus_tag="IWI57_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBC2450028.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWI57_001891"
     CDS             complement(3539..4513)
                     /locus_tag="IWI57_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBC2450029.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="IWI57_001892"
     CDS             complement(4891..5610)
                     /locus_tag="IWI57_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2450030.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWI57_001893"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWI57_001893"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBC2450031.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWI57_001894"
     CDS             7161..8558
                     /locus_tag="IWI57_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2450032.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWI57_001895"
     CDS             8639..9079
                     /locus_tag="IWI57_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBC2450033.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWI57_001896"
     CDS             9227..9553
                     /locus_tag="IWI57_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBC2450034.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWI57_001897"
     CDS             9554..10303
                     /locus_tag="IWI57_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450035.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWI57_001898"
     CDS             complement(10383..10838)
                     /locus_tag="IWI57_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBC2450036.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3532 a         1948 c         2290 g         3359 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacttgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtg
//
LOCUS       DADXTG010000084        10886 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.085, whole genome shotgun sequence.
ACCESSION   DADXTG010000084 DADXTG010000000
VERSION     DADXTG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.085"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..1054
                     /locus_tag="IWI57_001899"
     CDS             83..1054
                     /locus_tag="IWI57_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBC2450037.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1132..1368)
                     /locus_tag="IWI57_001900"
     CDS             complement(1132..1368)
                     /locus_tag="IWI57_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2450038.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1337..1468)
                     /locus_tag="IWI57_001901"
     CDS             complement(1337..1468)
                     /locus_tag="IWI57_001901"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2450039.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1544..1927)
                     /locus_tag="IWI57_001902"
     CDS             complement(1544..1927)
                     /locus_tag="IWI57_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBC2450040.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(1960..2745)
                     /locus_tag="IWI57_001903"
     CDS             complement(1960..2745)
                     /locus_tag="IWI57_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBC2450041.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            2923..4023
                     /locus_tag="IWI57_001904"
     CDS             2923..4023
                     /locus_tag="IWI57_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBC2450042.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4013..4837
                     /locus_tag="IWI57_001905"
     CDS             4013..4837
                     /locus_tag="IWI57_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBC2450043.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            4834..5649
                     /locus_tag="IWI57_001906"
     CDS             4834..5649
                     /locus_tag="IWI57_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBC2450044.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5646..6476
                     /locus_tag="IWI57_001907"
     CDS             5646..6476
                     /locus_tag="IWI57_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2450045.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6483..7514
                     /locus_tag="IWI57_001908"
     CDS             6483..7514
                     /locus_tag="IWI57_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450046.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7621..8894)
                     /locus_tag="IWI57_001909"
                     /pseudo
     CDS             complement(7621..8894)
                     /locus_tag="IWI57_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9171..9380
                     /locus_tag="IWI57_001910"
     CDS             9171..9380
                     /locus_tag="IWI57_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450047.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9430..10146
                     /locus_tag="IWI57_001911"
     CDS             9430..10146
                     /locus_tag="IWI57_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBC2450048.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10048..10568)
                     /locus_tag="IWI57_001912"
                     /pseudo
     CDS             complement(10048..10568)
                     /locus_tag="IWI57_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3038 a         2087 c         2535 g         3226 t
ORIGIN      
        1 tatatgagct aattttatga ttaaatggta agtttttcct tgtgtgtttt agtttaaact
       61 agtgtgtaat tcaaaaatct atatggaagg aacagtatta agaatagaac agggttctct
      121 acatgatggt gctggtttac ggaccgttgt ttatttaaag ggctgcccat tacgctgtgc
      181 ctggtgttcg attcccgaat cacaaagcaa gcagattgaa aaaggatttg gccagaccat
      241 gaccgctgaa gaggtaatgg atgagattga gaaggatgct gttttttact ttcactcgga
      301 tggcggagtg accattagcg gaggcgaggc actggttcag gcggattttg ctaaagaaat
      361 tttgcaaaaa tcgaaataca ttggaatcaa taccgtactg gaaaccagct tttgtggtgc
      421 ttataatgaa atccaaaagg tagctcccta tgtggataca ttgtttgtgg atgtaaaaat
      481 gtttaccagc aagctccaca aacaatggac cggcttagat aaccaacaaa ttttgaagaa
      541 tattcgtcgc tttttaattg agtatcctaa ctgcgaagtg cgaattcgag ttcctgtggt
      601 tccaggtatc aatatgaacc tgaccgagct gctgacgatt gcttgttttg tggcagatct
      661 tgatcggttc gtaccgttag aattactacc gtaccactgc tatggtatgc acggttacca
      721 agcattggga ctggagtatc cgttggcaga cacgccagcg ccttcaccta aagaaatgtt
      781 tgcattggca gatcaactgg ctcgcacggt tcctcatttg ctggtcacag ctttaaccaa
      841 aacatttatt tatttattga atccaagctt tatcttatgg ataaatgaaa gacactcact
      901 agctgcagcc agctcaagaa tagagctttt gctgaagtca gtgagtgtct ttttacgatg
      961 ttttttcgaa gttaatttgt cgaatgaaca ctggctcaat cggtacatct tcactgtttg
     1021 tcggctgagc tgcgatcgct gctacgattt ctaacccttg gtatacctgt ccaaaaacag
     1081 tataacgacc atccagctcc ggtagtcctc caagctccaa gtatgcttgc tttacattag
     1141 caggtcagcc tgcgcctttg tgttttgcac aataaaaaac tggttgccgt tggtgtgggg
     1201 gccgtggtcc cctaaggcca aggcgccatt aaaatgataa agtccgcgat gcacttcatc
     1261 agcgaaaaag ccctcatagc tggaaacagc ttcatcacca gaaccatcca aagcgccacc
     1321 ttgaataaca aattccttaa tgacacgagc cgaaaggtgt gttgtcataa acgccttgct
     1381 cagctaggga gcgccagttg gctacagcca ggggagcttg gtcaggaaat aagcggagac
     1441 gaatttcacc agcatcggtg accagcacac aaacttcttc ctgctgcggt ttttctaatt
     1501 gaaagagagg aaggtttgca actgaaacgc cttgatcctg ccatcaaata tcgaaaatcc
     1561 gccactcgac cttcgcctga tcaccaaggc gagccattcc caataaaacg gtataggcgg
     1621 tttcatccgc ttgaaaggtc aattgcagct gtcctcgtaa atcgttgtag tcatctgggg
     1681 cggctacttg ataaaaaaga tgaaactgcg catgcaaata ttccaaaggc tgatctttaa
     1741 aggctgcaaa ataagcctga actttttccc gctgcaagct gtcagccacc aaaaaatttt
     1801 ccagttgttg ttttggaacg gctgctaaca aggatcggca aaccgcctca attttcaacg
     1861 catcccaatg tcgcgcaatt aaataatgga tgggtgcctc ttgattgtag tcccacaggt
     1921 tgctcatttt tttcaccact ttcttctatt atataggaat cacaaaatcg gcagcggcgt
     1981 caggcggcgt tcttttcctt gaatgactag caacttttct acctgacagt ctgccaacag
     2041 ctgactcatt tttttaaagc ccagctgata atcttcggca acatgagcat ccgagcctaa
     2101 ggtaaagagc tttccgccga cactttgata caaaggaacg gcataacggt aaaggtcttc
     2161 gttgccgtat ttcaagaagc tcttggcatt taactccatc gccaattctc gttcaacaac
     2221 ctttttgaaa atcgttagca gctgagcctc aaaatgctcc gcgaattctt ccgctgtgaa
     2281 ggtgaagcgg cgtaatccat aatcaaagtg agtcaaaata tctccttctg gaaaatccgt
     2341 caagacctgc aacatctgat caaaatattc cttggtagtt tgaatgggat ctttggttaa
     2401 aaccacatcg tccatataat caaagcggcc attttgatga atacttacca gctttaaatc
     2461 ataaggatgc tgagcgaggt aatccacgat ccgctcttct tgtcctggaa caacgccaat
     2521 ctcgatacct cgcagaaatt tggtggaata ggtttctttt aaagagtcca gtttgtttat
     2581 ataggctgga taatccggaa tgtcatcttt tccggtagat ggattgttta aatcaaagtg
     2641 atccgtagaa acgaaaaact ccggctgata ggctaaatag ttttcaaatt tttcctctga
     2701 atcaaaggaa aaatacgtgt ggagatgctg atcataatac tgcataggga gcctcctttt
     2761 ttctactatc ataccgcaaa agctttagaa ggttaacggg aattcgtcga aatttacaaa
     2821 ccatatacaa cctttacatt gttcatgaaa ccttttcaag aaactgattc tctcattaca
     2881 aagctttgtt agaatggaat tatcgaaagg ggagaagaat gaatgaaaat gaatgaaaaa
     2941 attcgtaaca tgactatcgg tttattttgt gcagcaacag taagtatcct gctgacaggc
     3001 tgcgtctcag gtgaagcaac gaagcaatcg gctgcggcag acgactcttt agatacgatt
     3061 atagaaaaag ccaaggaaga aggagagatt gccagcttgg gaatgccgga tacttgggca
     3121 aattgggtag gcacttggaa cgaccttgaa aaagaatacg gcttaagtca cgtggatacg
     3181 gatatgtcca gtgcagaaga attggctaag tttgaatcag aaggaaaaaa tggaaccgcc
     3241 gatattggtg atgtcggtat cagcttcggt ccgttggctg aaagcaaaga gttaacctta
     3301 ccttataaga ccaactattg ggaggaaatt ccggattggg caaaggatga tgatggggat
     3361 tggttgttaa gctatacggg aaccatttcc tttatcaccg ataaagagaa tgtgaagcaa
     3421 gcaccgacta gctggaagga gctgcaagag ggtgactatc aagttagcgt gggggatgtg
     3481 acgattgcca accaagcaca atttgctgta ttggctgcgg cgattgccaa cggaggtgac
     3541 gaaaaggata ttcagccagg tctgaattac tacaaaaaaa tggcagaaaa aggccgtttg
     3601 tccaccgttg atgccagcgt agcaaatctt gaaaaaggtg aaatcgatgt ggcagttgtt
     3661 tgggacttca atggactaaa ttatcgtgac caaattgact ccgagcgttt tgaggtaact
     3721 gtgccaacag acggctccat cattagcggg tatacaacct taatcaataa aaatgcccct
     3781 catcccaatg cggcaaagct agcacgggaa tatattttat ctgatgaagg gcaaatcaat
     3841 ttggcaaagg gctatgcgcg acctattcgc aaggtagagc tgcctaaaga agtagctgaa
     3901 aaattactgc cggaggatgc ttatgaagcg gcggtgccag ttgaagacaa tgaagcttgg
     3961 aacaagacct ctgtagagct tccagagctt tggcaggcgg aggtactgac ctatgcaaag
     4021 tagacttgac aaactgattg tagttatact agacggctgt cgctacgaca cagccgtcgc
     4081 ccagcttggg ttcatgaatc atttagttga gcaccagcaa gcaagcttat tgaaggtaaa
     4141 aagtgagctg cccagcaatt cacgtccgtt atacgaagta ctgatgaccg gcgttccagc
     4201 ctatgaaaat ggcatctata gtaattacca tgctcagcga tccaaagagt ggtcgctgtt
     4261 tgaattagtt aaggaagctg gcggaaaaac aggagccgcc gcttaccact ggtacagcga
     4321 gttgtacaat caagccccct ataatcgttt ggttgatcgc atccagcatc aggaggagcg
     4381 actgattcag catggcatct tttatagtga ggacacttac cctgattcgc atttatttgc
     4441 ggatgcccat tatttagtga atcagtatca gccagacttt ttggtcgtac attcgatgaa
     4501 tattgatgat gtcggtcaca aatttaccgc cgattctcat gaatatgcag cagcggttaa
     4561 tcaggcggac agattattgg ctatttacct tcctgaatgg ctgcaaaaag gttatcaagt
     4621 cgtggtgact gcggatcacg ggatggatgc gcacggactg catggcggca gcttggctgc
     4681 acatcgtgaa gttccttttt atttgatttc aaataaacgc caaaagctga aggaacaaac
     4741 tgtaccacag ctgctggcgg ctccattgtt ttgctggtta ttaggaattg caccgtcaga
     4801 gaaaatgcag gacattcaag aattgatgag gggatgaaag agatgaaaaa acgcttggtt
     4861 ctggttcctg cctcctttat cttctgtgca tttttactgc tgccgctgct ttttatggtt
     4921 ctgtgcagct tgcacagtga tttaactaac cagtggacat tggccaatta cagtgatatt
     4981 ttcagcaaca aatattacac acaggccttt gttaacagcg tactgattgc tgtcagctcc
     5041 agtgtgattg ggttaatcgg gacgtttatt ttatgtttat gcttaatgaa tctatcgact
     5101 aaattgcagg agaaaatcac ctttgtttcc aatttggcag ctaattttgc tgggattccg
     5161 ctggcattct cttttattat tttgttgggg aatgtaggtg tgctgaaaat gattttgcca
     5221 tttctagcgg acttcaattt gtattcttgg tggggattga ccatcacgta tacctatttc
     5281 caaattccgt taggggtctt gtttttgtat cccagcatga aggaagtgaa aaaggaatgg
     5341 ctggagtccg cacaattgct gggcgcttca tcagcgtatg cttggttcaa ggtggctttg
     5401 ccttttttgc gaccatcgat tgccagcacc tttgtcatct tatttgctaa tggcatgggg
     5461 acctatgaaa cagcgtatgc cctgaccagc agtaatgtaa atcttttaac gattcgaatt
     5521 gcggctttag tgtcgggaga tgtgttcgcc aagcctaatt taggcagtgc gttggcagtc
     5581 gctttaggtg tcctgctggt aacggcaatg ttgattattc aaaagaaagg caaggtggtg
     5641 cagacgtgaa aaagttaata aaaccgattt tgttctgctt tgtcctgtat tacttgctgc
     5701 cgattattgg aacgattttg tatgccagct ccactaaatg gtcaaaatcg cttttaccta
     5761 gtgattttac cttgcaatgg ttccaacagc tgctgacgga cagggaattt attgctgcgg
     5821 tgggtcgctc acttttatta gcaggtgtgg tcttggtgac gattctgttg ttgatgattc
     5881 ccaccattat ttggattcat ttgtatttcc ctcgtttgaa tcgttggctg gaaaagctgc
     5941 tcctgctgcc gtatgcttta ccaggagtga ttttggtaac ggctttgctt cgaacgtatg
     6001 ctgaaactgg tatcccaatg tttgtagtgt tggtaggtgc attgtttatt acggcgttgc
     6061 cgattgtcta tcttagtctt aacaaccaga tgcgcttaat taatctgaag gaattagtgg
     6121 atgcagcaga aaccttgggc gccccgatgt ctacgattat tatccaggtg ctgtttccaa
     6181 acatccgtat cggtgtgacc ctagtttctc tgatgatttt ttcttcggtt tttggggagt
     6241 acatgctgac gaacctgttg attggcggtc gatttcagac ggtacgcatc tatatgctgc
     6301 ggcggatgaa cgaaaacggc catttggcta gcgctgtgat ggttttgtat ttgctgttta
     6361 tggtgggaat tgctttaact acctttatgc tgacttatcg acaaaagcaa cagctgtccc
     6421 ttaagcagac gagggaagag aagcagtcga aaaagaagcc cttaaaggag ttgtgacgga
     6481 aaatgttttt agaaatacat gatctcaatc tgttttttga tgaaaaacag attttagcta
     6541 ttgatgaaat tcagattgaa aagggtgagc tggttacttt actaggtccc agcgggtgcg
     6601 gaaagtctac gttgctgcgc tgtatcacgg gattggaaca accaaagagc ggtgagattg
     6661 tgctggacaa tgagaatatt gccgataagc ctacgaagga tcgcaatatc ggctttgttt
     6721 ttcaacagta tgcattgttt ccaactatga ctgtttttga aaatgtagcc tttggtttga
     6781 aggtaaagaa gctgagtcct gaggtgattc aggaaaaggt ttttgagatg ctatcattgg
     6841 tggatatgac ggagcaggca gataaaaatg tccagtttct ttctggcggt caacgacaac
     6901 gggtggcact ggctcgctca ttggtcactg aaccgaaagt cctgctgctg gatgagccat
     6961 taagtgcttt ggatgcccga attagaaaac agctgcagcg ggatttgcgc gctatccagc
     7021 aatcgctagg gatgacgatg atttttgtga ctcatgacca agaagaggcg atgcgaatca
     7081 gtgatcggat ttttgtgatg gaagctgggc gggtggctca ggtttccacc tcgaaggaat
     7141 tgtatcagaa tcctcagagc cgatttgttg cggaatttat cggcaactat aatcgtttcg
     7201 aatggtatga gctgaatcag tatacccacg agttttccgc taagggcaat cgctgcattt
     7261 attatttaag accggagctg attcaatttg aaccggttcc tcagggggtt aagattccgg
     7321 ttctatggaa ggaatcattt attttaggca acattcaacg gtatgttttt caaacgctgg
     7381 agggcaaaga aattttagtg gatcggttga acgatcggga agagaaaatt agcgatgcct
     7441 tatacattca gctgaaagat attctagcaa tccctgtgga aattgatact cagcattttg
     7501 cacaacaaag ctagtgtata gtcggcaaac atttctggct ctttgtcaaa taggactgat
     7561 agagtgatat aagaacatct aaggtagaat tgattttctg ctttagatgt ttttttatgt
     7621 ttaataagtg atcagatgat tgattaagaa aattctggtt ctcatcctaa cccgtaagct
     7681 attcgtttca atgctttgat gtgggtatgc agattttcta ttttgccatt tgagtaaggc
     7741 aactctaagg ctaaacgaat gccggattca tagtttataa acgctttttt tatatgatgc
     7801 agttctcttg gaaccgactt gggcattgtt tttacaagct gaaagaagat gtccgcttct
     7861 ttgtcataaa aagcttgaag aatttcttga tagtaagcat aagctattcg tagttcatca
     7921 gaagtagaaa gtagacgttc tacgacttca ctttcggtta aataggccca accaaaatta
     7981 cgccattttg tccatttatc ataaacaagc gtgtcctgtc gtttgatcag aagtcgagat
     8041 aaagccttca cttgtcgata gtgtttggct tgtattggat cgtttcggtc gaagcttttc
     8101 atgactcata gtcaaatatc cttaaaactg cgaatgaggt gcttgacgat gtgaaagcga
     8161 tccacaacga gtttggcatg aggaaagcac tcttttatca gcaaagcata agaggcgttc
     8221 atatctgtta cgagtatttt tacgtttaaa cgtgcacgtc gttcaaattg cataaaataa
     8281 gaaatgagat ctttcttctt acgtgaagga agaatatcca gtaatttccc agtatcgcca
     8341 tccatacaag aaaaagaata gggaccgact tgatttttca atgaacgaaa ttcatctacc
     8401 aatgaacagc gaggcaacca atttattttc acgtttgtca tggggagcag agaagcgagt
     8461 gttcgcgaaa cggttgacca agaaacgccg caatgtttag aaatcgtcgt cattgcttgt
     8521 ttttcagtca attcctccaa tattttataa tgtacttgtt tggcgataaa acagtttttt
     8581 tcaacaatgt aggtattcgc cgtaaaatag gtgtgacagc cacgacagag gtaccgttgc
     8641 tttgccaaag caagataagt ccgttgattc gcacacttat tgagaaggac cagcgatttt
     8701 ttctttccgt tcttaacgat cagcccatgt tgattcacct gtttacactg aggacagggc
     8761 tgaatcgttt taatgagcgt ggcagaaaca acgatactgt tgattgaatg tatttttttc
     8821 ttctcgacat gagtgatttg aatgtttcca tctttgattc ctagtaatag tttgataaga
     8881 tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa ttctaacgga
     8941 gcttctgtat gttttgtttt tttatgctca aaaggactga tagaatgatt tactatcagt
     9001 cctagaaatt atagcgccac atttctttca cctttgactt ttcagacggc agtttttatg
     9061 ctgatactgt agagagagca ggacacagct tttgcttgtt ttgggtaagg gggaaagaag
     9121 atgaccaaag cacttatttt tgattttgat gggacgatga ttgacagtga atggatgttt
     9181 tacgagagta ttcatcacta tttactgaag cctatttact ggagcagttc ggctataaaa
     9241 ttccttttgc agactatcaa caaggtgttg ggacggcagc agaactgtcc ttttatgaag
     9301 ggataagtca agcggttggg agaccagtgg atgctgaaca ttttgagcag cggatcagca
     9361 gcactggaaa atcggagtag tctcaattct gaactgcggg agctagacta tttttttgaa
     9421 gcacatccga tgattaagga aaaaattgat tcctttgtga cgatcgatga tcttcaggtc
     9481 ggcaaaccag atccagaagg ttatttgttg tgcttgaaac ggctgagggt tgaacaggct
     9541 gttgtagttg aggatagtcc agtcggtgcc caagcagcac cgcatatgac agttgatgta
     9601 caagcgttgg atgcagattt tttcgccttt agcgggcata aaatgtgcgg gccgacagga
     9661 atcggtgtat tatatggcaa acgtcaattg ttagaacaaa tggagccagt cgaattcggc
     9721 ggtgaaatga tagattttgt caatctttac gatagttcat ggaaagagct gccatggaaa
     9781 tttgaagctg gtactccgaa cattgcagga gcaatcgatt acttgaatga gattggtatg
     9841 gagaatattc atcgttatga gcaagaactg gttgcctacg ttttacctaa attacaagcg
     9901 atcggcggta tcaccattta cggaccgcaa gatcctgaac atcatactgg cgtggttgct
     9961 tttaatttgg atggcatcca tccccgtgat gtggcgactg ctctggatat ggaaggtgtc
    10021 gctgtacgtt caggacacca ttgtgcacaa cggttcatcg acgatttctg tttgactaat
    10081 tggtcgttta aacagtcgat gataatgccg tttgagcggc tccaatgtcg tggaatcttt
    10141 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagattacgt
    10201 gattgatttt cgtacagttc tatgagaatc gcctgcttca gttcctttgt tagttgatga
    10261 tgttcatcaa ctaagtccgt tttcgcagaa aaggtctgcc cacaatcata gcatagaaaa
    10321 cgggtacgtt ttaattctaa aatagtcaac ttttctttga ctttcaacat ctgagtacgt
    10381 gtcgtatagg aaccgttgcg gataatacga atcgatccac agtgttcaca atgggaaggt
    10441 gagtcatcca atctcccttt aataaactgc gcttgaacgc ctttatattc tttttcaatc
    10501 agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt tctagtatga
    10561 ttatccatag agcgactcct ttggtagggt gatgttttgg tcgactaaat tttaccaaaa
    10621 gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt
    10681 tccaacaaca caaaatatta tagaagcctt ttttctaaag gatttcaagg tgtctgtttg
    10741 catttattaa aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca ctagatatta
    10801 tagagccata atatgctgta taatatctag tgtataggct taagtggagg tatatatgaa
    10861 aatattaact gttgaagatg ataata
//
LOCUS       DADXTG010000085        10867 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.086, whole genome shotgun sequence.
ACCESSION   DADXTG010000085 DADXTG010000000
VERSION     DADXTG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.086"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(126..1229)
                     /locus_tag="IWI57_001913"
     CDS             complement(126..1229)
                     /locus_tag="IWI57_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBC2450049.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1451..1921)
                     /locus_tag="IWI57_001914"
     CDS             complement(1451..1921)
                     /locus_tag="IWI57_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBC2450050.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2178..3497
                     /locus_tag="IWI57_001915"
     CDS             2178..3497
                     /locus_tag="IWI57_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBC2450051.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3938..5578
                     /locus_tag="IWI57_001916"
     CDS             3938..5578
                     /locus_tag="IWI57_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBC2450052.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5732..7054
                     /locus_tag="IWI57_001917"
     CDS             5732..7054
                     /locus_tag="IWI57_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBC2450053.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(7116..7547)
                     /locus_tag="IWI57_001918"
     CDS             complement(7116..7547)
                     /locus_tag="IWI57_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2450054.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(7611..8447)
                     /locus_tag="IWI57_001919"
     CDS             complement(7611..8447)
                     /locus_tag="IWI57_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBC2450055.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(8637..8939)
                     /locus_tag="IWI57_001920"
     CDS             complement(8637..8939)
                     /locus_tag="IWI57_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBC2450056.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(8965..9396)
                     /gene="ruvX"
                     /locus_tag="IWI57_001921"
     CDS             complement(8965..9396)
                     /gene="ruvX"
                     /locus_tag="IWI57_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBC2450057.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(9393..9659)
                     /locus_tag="IWI57_001922"
     CDS             complement(9393..9659)
                     /locus_tag="IWI57_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBC2450058.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(9913..10638)
                     /locus_tag="IWI57_001923"
     CDS             complement(9913..10638)
                     /locus_tag="IWI57_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBC2450059.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
BASE COUNT         3207 a         2265 c         1901 g         3494 t
ORIGIN      
        1 agtatgaaaa aatatttcaa ttttattttt tcgtttcaaa ttattctatt agtgacatcc
       61 ttgaactata aaaataggag tacggcatca tctgccgcac tcctattttt ttcgtcattt
      121 gtttcttaaa taaaacgatt ctcacgcaat tttatttttt tgatatcttc tactgtttgt
      181 gttccagcta attgcatgac catttccaat tctgttttaa agaaatcaaa cacttgatgg
      241 actcctgtac ttcctcctaa agacaatcca taaataacgg gtctgccaat agcaacaaga
      301 tctgcacctg atgcgattgc tttgaaaaca tgctgtccgc gtcgtacacc gctgtcaaac
      361 acgattggta cacgtttatc tactgcttct gccacatatt gcaacgaatc aaacgctgct
      421 ggaccaccat ctagttgacg gccaccatga ttagataccc agattccgcc agcaccagat
      481 tctaatgaac gatagacatc ttcttctgac tggacacctt tcacatagac tggcaaatca
      541 gaatgagcag caataaattc tacatctttt ggactaagct tctgtttgga agatttgtac
      601 acagcatcca tcgtttggcc tactcctgat tgataagcct gaacgatcgg cattggcagt
      661 gggaaggtaa acccatttct gcggtctgtt tcacggtttc cgccaactgt tgcatccgct
      721 gtcaatacga ttgcttttgc tccattacgc ttggccactt caagaatatc caaattgatc
      781 ccatcatctt tgctcatata aaattgaaac cattgcggtg ctttctcccc gcctgcttct
      841 cttatctctt ctaatgtaca agaagcgtaa gaacttgctg tatagatcgt cccaaaatct
      901 gctacacctt ttgcagaagc tttctccgcc ttcacatgag ctaagccatg agcagcaact
      961 ggtgccatga tgattggtgc agtcagcgtt tctttgtcaa aatgtgtagt tgtatcgggt
     1021 aattctacat ctcttaatac atgaggaata atcagctgat gattgaatgc ccgttcattt
     1081 tcttgataag taaataagtc gccagcacca ctgctgatgt aaccataacc accagctgga
     1141 atcacttgtg aagcggctat ttctaaatct tccatattga taaaatccac tattccttca
     1201 tttgtacctg cttgatacgt tttttccatt tcaatgcctt ccctctataa aaaattgaat
     1261 gaataatatg atagcgttta cacactttat attactcttc cgtattgata caggcaaata
     1321 atctgtttac atttcagtca ttccagttgt tactactctg aaagaaatgt ttagaacaca
     1381 aatagataaa aaacgatggg attttgacaa aagtcgtgtc aaagtccctt ttacgaaata
     1441 ctgtggatgc ctattgaaat aagtgcgggt tgtttctttg tatttcaatc gtttcttgcc
     1501 attcttcgtt taattttgga ttaccgtttt cttccttgaa cgcataaggt cccaatccaa
     1561 aaagctcagt tagtatttga ttcacgattc catatagctc ggtgtgatcg gacacatgat
     1621 tattgaagat catctcatta tcaccacgtt tgaatctcac attcgggctc tcatgatgaa
     1681 cctctatgca gttttcccct ttttttaact gacggatttc tgaaaacatc tcataaatat
     1741 cttctccaat aaaaccttca gcaagcgcca gactgtccac aagaccgcct actgtcaact
     1801 cagagattat cgctttgttg gcaccaagct ttaatgtata gcttgttgct tctactacgc
     1861 cgcatgcatt gcaaccacct tctgatagtt cgctttttct gatcaattga acttctttca
     1921 ttttctgttc ctcctcttag ttttctactt atgtattttt tatcacctga cgtaagtgta
     1981 ttttatcgtc acgatcacgt gatttattct tcagagattc tacctgaata attcatactt
     2041 ttaattaaaa ccatgtgaaa ctgcctcacg gcagcttaca attacttttt catcttcacc
     2101 cgttaagtga tgaaaaaaga acccctttct gctatggtta aagtgactaa accaaccaaa
     2161 agaaaggagt tcttttcatg actagcttac acaaaaacca agtaaaattc aattcaaata
     2221 tcactatttc tcatacaggt ggtcaattat cgagtgattc gggtctcgtc ctagtgaaag
     2281 agctgatgaa catctttggt ttttctgaac tggctaagca acacattcac attgaagacg
     2341 aacgagcgta ttttactcat gacaacttat ccatacttga gcagttcatt atgcaattaa
     2401 ttgctggtta ctcagctgat tctgcagcta atctcctgag acaagatcct gtgtttaaag
     2461 ctgttttaga taggaaagaa ctcgcttctc aatcttcact ttcccgattt ttagatcgac
     2521 tatctgagga gaatatccat gaacttcaag ctttgaacca agaacttatc gataaagcac
     2581 gcctcatccg taatgatacg gaattaatca ttgatttaga ttcgacccat tcagatacat
     2641 ttggtcacca agaacaaacg aattataata cacactacca aacctatggt taccatccat
     2701 tggtagcttt tgacggattg actggtgact tcttaaaagc tgaactacgt tcaggaaatc
     2761 aatatacatc aaaaggcgta aaggagtttc tcacaccttt attagagcac tataatcact
     2821 ctctaccaaa cactgacatc ttggttcgtg gagacagcgg gttcgctaca cctggtgtgt
     2881 atgattcgtg tgaatcaaaa aagagtcatt atgttattcg actgaagaat aatcgtagac
     2941 taggtcaaat agctgagaaa tcagtacttt atggcgataa tcaaaagtgg gaagaacgag
     3001 aagttcagta cttctcgacc acttatcaag cacaatcctg gtcacaaagt cgtcgcgtgt
     3061 gtatacgctc aacacgtgaa gcgggcgaac tactctttcg acatgaattt atcgtgacga
     3121 atctatcaga aaatgtttct cctgatacca tcttttctat ctatgccaaa cgtggcacaa
     3181 tggagaactt cattaaagaa gcgaaagctg gcttttactt tgacaaaaca gacagtcctc
     3241 gctttttgga gaatcatgtc cgaatgatgc tcagcttgat agcttacaac ttagtcaact
     3301 tcttaagaac gattggcttt gaagaagtcc aaaagggaat gaccattcat tctattcgat
     3361 tgaagtttct gaaagttgct gggaaattag tccaaacggg tagacgagtc tatctcaaat
     3421 tatctagtta tcatgtgtat cagaatgaat tctacagggt ctttgctcgc ctgaggcgag
     3481 ccagtcaatg gatctaatcc aacaatctaa cgattttttt actgggaagt tatccaggga
     3541 gaagtatgct caaaatgcct tacacccaaa atttattcct caatattttt gaacagtgaa
     3601 tgtttgaagt aactttttag tcaaaatcag ttgataggga tattagtgat acatatttca
     3661 ataaaatgtg ccaaagaatt aaagctatga attattcagg attctattta tctatctaac
     3721 atatccttta ttcttctact tttctttctt tagcatataa gagatacgca tagtaatcaa
     3781 gcaaggattg ttgatataaa aagaacaaat accgctattt tgatttcttt tgactctatt
     3841 actcatataa ataaaattta ttatacctgc tctttttgac tatatcctca tctgactcct
     3901 atacttcgtt tgtattaaaa cttaggagga atcattcatg cttacagggc taaacttatt
     3961 aaataatcct tttctaaata aaggaacagc ttttacaaaa gaagaacggg caaaatacgg
     4021 aatcactggt atgcttccaa gtactgtcca aacattggaa caacaatctg tccaagctta
     4081 cggtcaatac ttaagcaaac agaccgattt ggaaaaacgt atcttcttaa tgaatctatt
     4141 caatacgaac cggacattgt tttataaatt gatgggcgaa catttagttg aattcatgcc
     4201 tgtcgtttat gatcctgtcg ttgcggattc aatcgaacaa tacaacgaaa ttttcttgaa
     4261 accacaagat gctgtatttc tttcaatcga cgaaccagaa aatatcaaag caagtttgaa
     4321 aaacgcagca gatggacgcg acattcgttt gatcgttgtg acagatgcag aaggaatctt
     4381 aggtatgggt gactggggtg tgaacggtgt tgacatcgct atcggtaaat tgatggtcta
     4441 cactgccgcg gctggtatca atcctgcaca agtcttgcct gtatcaatcg acgctggtac
     4501 taataacgaa gaattattga ataatccatt atacttaggg aatcgtcatg ctcgtgtaga
     4561 aggcgaaact tactacgaat tcattgatca attcgttcaa tcagctactg aattattccc
     4621 agaattgtta ttacattggg aagattttgg acgaggaaat gctgcaacga tcttagaaaa
     4681 atacgaagat aaaatcacta ctttcaatga tgatatccaa ggcacaggaa tcgttgtact
     4741 agctggtgta ttaggtggat tgaatatatc aggtgagcaa ttgaaagatc aaacgatctt
     4801 gacatttggt gctggtactg ctggtgtagg tatcgcaaac atccttttag atgaaatgat
     4861 ccgtcaaggt gtacctgaag aagaagcacg ccaacatttc tatcaagtag ataaacaggg
     4921 tttattgttt gaagatacaa aaggattgac tcctggtcaa atcccattcg ctcgtaaacg
     4981 ctcagaattc actaatgctg atgaattgac aaacttagaa gcagttgtaa aagcaatcca
     5041 tccaacgatc atgatcggta cttctaccca acctggagca ttcactgaag aaatcgtgaa
     5101 agaaatggcg gctcataccc cacgtccaat catcatgcct ttgtctaatc caacaaaatt
     5161 ggcagaagca aaagcaaaag atttgattga atggacggac ggaaaagctt tagttggaac
     5221 aggtatccct gctgctgatg tggaatataa cggtgtaact taccaaatcg gtcaagcaaa
     5281 taatgcgttg atgtacccag gtttagggct tggtttgatt gcttcaacag ctacacgagt
     5341 aaatgctgaa atcatttctc aagctagtcg agcattaggc ggaatcgttg atgtaacaaa
     5401 accaggagca gctatcttgc ctccagtagc aaaaatcact gaattctctc aaacgattgc
     5461 tgaaactgtc gcaaaatcag ttgttgcaca aaaattgaat cgtgaagaaa tcacggatat
     5521 caaagaagct gttgaatctg ctaaatgggt tcctgaatat aaatctttag aagattaaga
     5581 ttgttatagt aaaagaacat acagaaactt tgtatgacgt ctaaaaagaa aaaagcgact
     5641 gcggcaaaac agcagccagc caactactgc tcttttgccg cttctctttg gcttttttca
     5701 tacgatacag aataagaaag gatctgtaaa aatggcacaa aaaacaacaa ccgcaactgc
     5761 agttgaggaa aagaaaaaag aagggttttg gtcaacaaaa attaccggcg taagtttgcc
     5821 cctttatttg atcatggtcc ttgtcctgat cattgcaatg gctttaggaa aattaccgac
     5881 gaatatgatt ggcccaatcg ctgttttggt cattttcggt aatttatttc attttatcgg
     5941 aacaaaaatc cctatagtca aaagttattt aggtggtggc tccgtttttg cgatctttgc
     6001 ctctgccgcg attgctacat ttgggatctt acctgagtat gtagtaaaat ccacagaaaa
     6061 ctttgtcagc aatatgggct ttttagactt ttatattgct gccttgatta ctggtagcat
     6121 ccttggtatg aaccgtaatc tattgatgaa agcttctgtt cgttttattc ccgttgcact
     6181 tatttcaatg gtcgtctcct tttttgccgt aggactcgta ggtatgctta tcggaaatgg
     6241 ttttgcaaat tccgttttat atatctcatt gcccgcaatg gctggtggtg ttggagccgg
     6301 agctgtacct ttatcaacga tttatgcaaa tgttttaggt acggacgctt cttctatcat
     6361 ttcccgcctg attcctgcga cagcaatgac aaatgtccta gctatcattg gtgcagcgtt
     6421 agttgctcgt gcgggacaat caatgccaaa attcaacggt aatggtaaat taatggaaaa
     6481 aggcgacctt ggcgatggaa aacctagaaa tgtaaaacct tccattccac aattaggtgt
     6541 cggtttgatg gtatcattga ctttcttcat cttaggacaa atctgtaact attttgttcc
     6601 taaagtccat gcttacgcat tcatgatcat tatcgttgtt atctgtaaaa tcgcaaacct
     6661 attaccagaa tattacgaag atgcagctat catgttcaac aacttgattg tcaaaaactt
     6721 gacagcagct gttcttgccg gtatcggtat tgcgttattg aatctaaatg tattggcttc
     6781 tgctttgaca tggcaatttg ttgtcttatg tttgacaagt gttatcgcga tttcagtagt
     6841 ttcaggattt gtcggtcgtt tgtttggttt atatccaatc gaatcaacta ttactgctgg
     6901 cttatgcaac aactcgatgg gtggtacagg aaatgtagct gtcttatctg cagctaatcg
     6961 gatggaactt attgcttttg ctcaaatggg caatcggctt ggtggtgcca tcgttttgat
     7021 catctcaggt ttcttgatgc aattactttc ttagtcaaaa aaaatacctg atcatcaagt
     7081 atcggcttga tgaccaggta ttttttgata aaaatttaca ggattttcaa aaatcttttt
     7141 tgcattttat cggaatgata accgatgcgt tcataaaatc ggtgtgcttc tactctgtat
     7201 tctgcagaat taagtcttat tgctgatatt cctcgtcttt tcgcttcttc tacgccagtc
     7261 atcaaggcct ttccgattcc tcttttttca gcatgtttag acacagcaag tgccataata
     7321 ttgaacatcg gatcgctgaa aatcgattca taggactccg cttgtacata gccagctgct
     7381 tttttagaag taggatcttc atagaccaag aaaaaatttt tttctggctc cttcaacagt
     7441 ttcttcattt gtctcaccgt aacggttaat ggtacatcat atcctaattg ttccgcattg
     7501 atttctcgta gtgcttctgc atctcctaaa cgcatttccc gcaacatcca aatcaccagc
     7561 caatttttat ttttttaaat gaattttttt atcacagtat actccttttt tcacttaaaa
     7621 aaagactttt cagtgatttt gacttgcttg aatccagatc cttctaatgt ttgcaagatg
     7681 acgaaagcat tgtaaagatt gatcaaaaga accacgatga caatcgcttg aaaaataaag
     7741 gtcacttttt ttggagacaa aaccctgcta aatcttgctc cccacaaacc tccaataaca
     7801 gcagcgggga taacataaaa caacatagat aaatcaaagc gcataaagct acttgttaaa
     7861 gcaatcgtta ccaattttgc cagttgagaa aagaaaatcg tagatagaga atatagtgtt
     7921 gcttctttta ttggcaatgc aaacatcagc atcaacaaag aaacattgac tggtccacca
     7981 ccaataccta aaaaactagc taaaaagccc agaacccccc cacatataag gtaccaagtc
     8041 catgacgtca aatgaaattt tggctgatga tgcttggtgt agaagaacgc aaaaaccaag
     8101 gtcaataccg tcaaaaaaat ttggatcagc tgcacatgct tctcgttttc aaataactgt
     8161 aaaaaaactt cgaatacaat gttgccgact actcctccaa caacagctcc actagatacc
     8221 caaccgacaa tctgccaatt cagtgatttc ccgcttgata cttgtcgcac agtcgaaaca
     8281 atggacatcg tgaataccgc aactgttgag taaaaagaaa tccctgcaac agaatgggcg
     8341 ccgataagat caagaatcgg tttgatcaac acgcctccac ccatcccgga aatagcgcca
     8401 actgtgttcg ccagaacgat caccacaaaa taaatcagac caatcatctt gtatttcctt
     8461 tctcttgttt atatctttta gcgattgtta ctcttttctt ttattataca gtaaaagaaa
     8521 caaacgaggc ttcgaaagct tttctacttt ttaaggaaat ttagtgctaa acgaaaaaac
     8581 cgggctctac ttttcagaga gtccagcttt tcttacacct tatcgattct tttgttctat
     8641 tcttcttccg ccatgaatgt attgaatact tcttcgatca tatcccattc tgcgtctgtt
     8701 tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt
     8761 aattctacat cttcatcttc tgaaacacca gctggatata aaagaacata gttacgtcca
     8821 aattcttctt gtccgtcaac tgttagcaag atttcgtata atgtttcatt tccttggtca
     8881 tccaccaatg tgatgtgttc gtgtccttcg tgttcgtggg aatggttatg ttctgtcata
     8941 agattacctc ttttcttttt attattaaaa taattgctta cctgagccgt ctaaataatt
     9001 ttgtaaaatc atcacagctg ctaatttatc gatgactttt tttcgtttcg ctcttgaagt
     9061 atttgcctgc tctactagca tacgttctgc ctgaacagtg gttagtcgtt catcttgata
     9121 atcaaccggc aaggagaata gttctttgat cttctctcca tatgacatgg aagcttctgc
     9181 ccttggtccg atggaattgt tcatattctt aggtaatcca actacaaaac ggtctacttc
     9241 gtactgcgcc actaattcct tcaaccgatc aaaaccaaac tcttcttctg cctcattgat
     9301 tcgaatgatt tctactcctt gagctgtcca gccaaaggga tcgctcacag caacgcctac
     9361 tgtcttagaa ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg
     9421 tagtctttga caattacttc catgatttca tcgcgttcgt gacggcgaat caaatttcgt
     9481 gcatcacgat aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga
     9541 ttgattggat tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc
     9601 acttcttttt tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg
     9661 attctacacc tctttcccgc aaggtaattc cagcttacct tctacaccct tattttactc
     9721 gaagaaaggg aaaactacaa acaaatattc caaacttctg aataaattaa gaaacacgat
     9781 ttgtctgatt tttccttttt tctgtggctt tttcaaaagc cctctttcca taaagtatac
     9841 gctatatttc tttgaaagta tgtttttaca caattttttc ataaagttca taaaaaattc
     9901 gaattgctgc tactaatttt tcgttttcgt tgcttgccct gtctcataat tgatggtgta
     9961 gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt caatgctttc
    10021 tgcttccaac tgcacacctc ttgctaccag ttcatttccc tcgaaatgag gagtgacacg
    10081 atagcgtaca tgatgatttg tttccttgat atatgccgca atatcatttt cgtgggcaag
    10141 catgtacgga gcattcaacg aacgtgtccc tgtcattaag tttttaatgt tattattttg
    10201 accagtcaac tggaaaccaa tcaaatggct gcggttgtat aaccattgtc cgtcacttaa
    10261 tttcttttgt ttccaacctg tcggatcaat atacaaaggt tctctctttt cagtaggaaa
    10321 caagtctttt cccaacatcg cattggcttg tcctactctg ttcaatttgt ctatatttga
    10381 atagctttcc catgtccctt tggaaagact aagatcctct tttgtaaact ccggctgatt
    10441 cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg ctacttgaga
    10501 agcttgcggt gtggaatcaa aaatggttgt aatttgttgg aagatatctg aatctttttg
    10561 tgtggtggaa ataccgagtg cccctgcaat cacagcagcg acgatcccca cgatcgcctt
    10621 ggttgttttt ttgctcattt ctttactcct ttactaacag ttccctaaat cttaacatat
    10681 gttcccctat aaataaatac tggaaagaaa tagaaacagt aaaaaaataa aaccagtttt
    10741 tagacggctt aagaatacaa aaaaagagac ggattcatcg tctctactca atcacaaaac
    10801 actatttaca aggtatttat tatcattcaa aaaatttttc ttaatactca aaactaaacg
    10861 gcttctg
//
LOCUS       DADXTG010000086        10518 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.087, whole genome shotgun sequence.
ACCESSION   DADXTG010000086 DADXTG010000000
VERSION     DADXTG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.087"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..656
                     /locus_tag="IWI57_001924"
     CDS             33..656
                     /locus_tag="IWI57_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450060.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1105..2154
                     /locus_tag="IWI57_001925"
     CDS             1105..2154
                     /locus_tag="IWI57_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2450061.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2151..3032
                     /locus_tag="IWI57_001926"
     CDS             2151..3032
                     /locus_tag="IWI57_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2450062.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3035..3805
                     /locus_tag="IWI57_001927"
     CDS             3035..3805
                     /locus_tag="IWI57_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2450063.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4084..4938
                     /gene="aroE"
                     /locus_tag="IWI57_001928"
     CDS             4084..4938
                     /gene="aroE"
                     /locus_tag="IWI57_001928"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBC2450064.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5116..6279
                     /locus_tag="IWI57_001929"
     CDS             5116..6279
                     /locus_tag="IWI57_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBC2450065.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6396..6806
                     /locus_tag="IWI57_001930"
     CDS             6396..6806
                     /locus_tag="IWI57_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2450066.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6980..9208
                     /gene="topB"
                     /locus_tag="IWI57_001931"
     CDS             6980..9208
                     /gene="topB"
                     /locus_tag="IWI57_001931"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBC2450067.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9379..9663
                     /locus_tag="IWI57_001932"
     CDS             9379..9663
                     /locus_tag="IWI57_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450068.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9823..10059
                     /locus_tag="IWI57_001933"
     CDS             9823..10059
                     /locus_tag="IWI57_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBC2450069.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10056..10454
                     /locus_tag="IWI57_001934"
     CDS             10056..10454
                     /locus_tag="IWI57_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBC2450070.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3666 a         1766 c         2229 g         2857 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga
      901 gactttccca tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac
      961 atgaacattg actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac
     1021 cgctttggcc attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa
     1081 gattgacttt gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt
     1141 attttagttt atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt
     1201 acttcgacga accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat
     1261 ccggcattag atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa
     1321 gaaggagaaa atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc
     1381 acaatgagtc aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct
     1441 gccgctcaat cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg
     1501 gatcctgtgg gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt
     1561 gtttctgatc agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat
     1621 gcaaaaaaag tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca
     1681 caagcagaag aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc
     1741 actaatgaga ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt
     1801 ccattagata ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca
     1861 aatatccccg tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc
     1921 gggatcaatc aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt
     1981 ggaaaagaaa aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc
     2041 aatcaatctc aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc
     2101 aaaatcatta cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat
     2161 cagctatttc acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc
     2221 ggatcttaga ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct
     2281 gtgtgacagc tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg
     2341 ctggcatgat agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta
     2401 ttttagcagg gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa
     2461 caccaaatct ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt
     2521 tacctgatta ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg
     2581 tactgctatt cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg
     2641 aaacgatggc tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt
     2701 tgtcgaacgg tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag
     2761 atgtaaataa agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag
     2821 tgatcttcca agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca
     2881 tttatcagct attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt
     2941 tgttctccgc tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga
     3001 acttaaaaac gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa
     3061 tggcagaaaa gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa
     3121 ccttacaatc agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag
     3181 cacactgttc aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa
     3241 agatcagctg atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt
     3301 ccaagacccc aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc
     3361 acaaaaaaga ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga
     3421 ttttttcaga ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc
     3481 gggtgaatta tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc
     3541 tcctgagtta ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc
     3601 attaatgcga ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac
     3661 gcatcgaatg gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa
     3721 aatcgttcaa gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca
     3781 gttctttgaa gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt
     3841 gaaaactcat tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac
     3901 atatttgggt gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc
     3961 tttgctctga caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg
     4021 tactttttcg tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat
     4081 aagatgattt ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt
     4141 ttatccccat tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta
     4201 gctttccaag taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat
     4261 atgttaggtg taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag
     4321 gtaagccagg aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt
     4381 ttgattggct ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt
     4441 tcgatcaaaa agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact
     4501 gttcaagcag cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca
     4561 ttcgctcaag taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa
     4621 gttcgtgact ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta
     4681 gtcaatgcaa cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata
     4741 agcatgatgc atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt
     4801 ttattaaaag aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc
     4861 taccaaggag caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt
     4921 gaaaagctgt ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg
     4981 tctcaagaaa agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt
     5041 atcaaaaaat gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa
     5101 cgaggaggaa ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa
     5161 cctctgaagc gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg
     5221 gaaaatgagg aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg
     5281 aaccaatacg agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat
     5341 aaaaaatttt tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg
     5401 tggaaaaaaa tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt
     5461 tttattggta aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc
     5521 tttcatgggc aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca
     5581 cgcggagaag atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc
     5641 cataagccga aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga
     5701 caaaataaga tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta
     5761 gtagaaaatt ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa
     5821 aaagtgatcg atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat
     5881 ctggatggtg tagatattaa aaaagggggc cagaagatgg atggacatgt tcttttgcag
     5941 tacgcacgtt tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa
     6001 gtaatgaacg ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat
     6061 gcagctggta aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa
     6121 aacctctttt cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac
     6181 ggttcgtgga attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa
     6241 atgaatcaat ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga
     6301 agctgcgcaa ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct
     6361 tatggtagat ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa
     6421 atgagtcgcg tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta
     6481 tcaccacata cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa
     6541 atctaatcac gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc
     6601 cagaaggaat tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa
     6661 gaaatgaaga agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga
     6721 tgccactgga tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca
     6781 tcaactggca gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag
     6841 cagcaaacgg atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta
     6901 tctttttttt attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt
     6961 aagtaacgga ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa
     7021 agcctatgcc gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga
     7081 tccattgttt gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt
     7141 tgctccagga gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt
     7201 cccagaaacc ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa
     7261 acgagaattg cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga
     7321 agccatcgcg tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt
     7381 acgtttatgg atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca
     7441 acctggaaag aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg
     7501 gctgattgga atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga
     7561 tggcagtttt tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca
     7621 agaagaaatc aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac
     7681 aaacaaaggg cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga
     7741 gttaacgaag gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct
     7801 tgaagttacg aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt
     7861 gcagacaaag atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca
     7921 aggattgtac gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga
     7981 aaatgagcat cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga
     8041 agaagtacag acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca
     8101 agaacatcat gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct
     8161 ttccaaatta cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc
     8221 tgcagattat atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa
     8281 aagcatcgga aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa
     8341 aaagaaagaa gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact
     8401 gaaaagtatg gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac
     8461 agcaatgaaa actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga
     8521 agtagaagga atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa
     8581 gcattacatc gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg
     8641 taaggcagta gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg
     8701 gtacttgaga aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa
     8761 aaaattcatc ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc
     8821 agatgagatt agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg
     8881 aagatgtcca aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt
     8941 gaatccggaa tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac
     9001 gaatctcaga gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg
     9061 taaaaaaggg aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc
     9121 ttttccatgg atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag
     9181 aaaaaataat gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc
     9241 cgaaaagtga gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac
     9301 gttggggtag gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat
     9361 tcatttttaa attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta
     9421 aagtatcctg attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca
     9481 ccaacgttga ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat
     9541 aaaacgacaa gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg
     9601 gctatgatca ttatttattt tctaccagtt attacacaag tatttcatca atattttggt
     9661 taagaatacg agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt
     9721 cgtaagaaaa agcaaaataa aagtatatac attgattaaa agtatatact tggatataat
     9781 gtacttacta tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag
     9841 tataaaatta gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa
     9901 ttcatgggtg tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta
     9961 cgaatgatca aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat
    10021 caatatgaag acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa
    10081 ttatcaaaat caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa
    10141 aagatgcaaa tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag
    10201 aactctatcc tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac
    10261 atccttttca taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat
    10321 tgaataattg tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta
    10381 ccactagttc attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa
    10441 ccgcaataaa atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag
    10501 gaatatgctg attttttt
//
LOCUS       DADXTG010000087        10492 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.088, whole genome shotgun sequence.
ACCESSION   DADXTG010000087 DADXTG010000000
VERSION     DADXTG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.088"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..2313
                     /locus_tag="IWI57_001935"
     CDS             19..2313
                     /locus_tag="IWI57_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBC2450071.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            2306..2971
                     /gene="pgmB"
                     /locus_tag="IWI57_001936"
     CDS             2306..2971
                     /gene="pgmB"
                     /locus_tag="IWI57_001936"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBC2450072.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            2964..3971
                     /locus_tag="IWI57_001937"
     CDS             2964..3971
                     /locus_tag="IWI57_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBC2450073.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            3993..5012
                     /locus_tag="IWI57_001938"
     CDS             3993..5012
                     /locus_tag="IWI57_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2450074.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(5293..6660)
                     /gene="nhaC"
                     /locus_tag="IWI57_001939"
     CDS             complement(5293..6660)
                     /gene="nhaC"
                     /locus_tag="IWI57_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBC2450075.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(6769..8190)
                     /locus_tag="IWI57_001940"
     CDS             complement(6769..8190)
                     /locus_tag="IWI57_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2450076.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(8433..10310)
                     /gene="tdc"
                     /locus_tag="IWI57_001941"
     CDS             complement(8433..10310)
                     /gene="tdc"
                     /locus_tag="IWI57_001941"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBC2450077.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
BASE COUNT         3306 a         2200 c         2009 g         2977 t
ORIGIN      
        1 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
       61 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
      121 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
      181 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
      241 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
      301 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
      361 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
      421 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
      481 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
      541 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
      601 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
      661 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
      721 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
      781 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
      841 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
      901 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
      961 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
     1021 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
     1081 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
     1141 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
     1201 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
     1261 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
     1321 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
     1381 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
     1441 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
     1501 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
     1561 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
     1621 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
     1681 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
     1741 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
     1801 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
     1861 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
     1921 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
     1981 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
     2041 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
     2101 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
     2161 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
     2221 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
     2281 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
     2341 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
     2401 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
     2461 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
     2521 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
     2581 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
     2641 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
     2701 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
     2761 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
     2821 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
     2881 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
     2941 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
     3001 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
     3061 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
     3121 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
     3181 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
     3241 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
     3301 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
     3361 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
     3421 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
     3481 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
     3541 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
     3601 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
     3661 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
     3721 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
     3781 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
     3841 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
     3901 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
     3961 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
     4021 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
     4081 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
     4141 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
     4201 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
     4261 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
     4321 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
     4381 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
     4441 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
     4501 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
     4561 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
     4621 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
     4681 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
     4741 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
     4801 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
     4861 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
     4921 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
     4981 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
     5041 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
     5101 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
     5161 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
     5221 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
     5281 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
     5341 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
     5401 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
     5461 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
     5521 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
     5581 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
     5641 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
     5701 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
     5761 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
     5821 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
     5881 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
     5941 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
     6001 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
     6061 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
     6121 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
     6181 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
     6241 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
     6301 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
     6361 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
     6421 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
     6481 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
     6541 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
     6601 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
     6661 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
     6721 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
     6781 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
     6841 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
     6901 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
     6961 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
     7021 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
     7081 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
     7141 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
     7201 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
     7261 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
     7321 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
     7381 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
     7441 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
     7501 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
     7561 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
     7621 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
     7681 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
     7741 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
     7801 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
     7861 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
     7921 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
     7981 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
     8041 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
     8101 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
     8161 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
     8221 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
     8281 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
     8341 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
     8401 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
     8461 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
     8521 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
     8581 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
     8641 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
     8701 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
     8761 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
     8821 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
     8881 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
     8941 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
     9001 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
     9061 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
     9121 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
     9181 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
     9241 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
     9301 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
     9361 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
     9421 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
     9481 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
     9541 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
     9601 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
     9661 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
     9721 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
     9781 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
     9841 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
     9901 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
     9961 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    10021 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    10081 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    10141 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    10201 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    10261 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    10321 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    10381 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    10441 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact cc
//
LOCUS       DADXTG010000088        10467 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.089, whole genome shotgun sequence.
ACCESSION   DADXTG010000088 DADXTG010000000
VERSION     DADXTG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.089"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..902
                     /locus_tag="IWI57_001942"
     CDS             69..902
                     /locus_tag="IWI57_001942"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBC2450078.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            1099..3027
                     /locus_tag="IWI57_001943"
     CDS             1099..3027
                     /locus_tag="IWI57_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBC2450079.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            3165..3620
                     /locus_tag="IWI57_001944"
     CDS             3165..3620
                     /locus_tag="IWI57_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBC2450080.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            4090..4359
                     /locus_tag="IWI57_001945"
     CDS             4090..4359
                     /locus_tag="IWI57_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450081.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            4759..5013
                     /locus_tag="IWI57_001946"
     CDS             4759..5013
                     /locus_tag="IWI57_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBC2450082.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(5102..6148)
                     /gene="holA"
                     /locus_tag="IWI57_001947"
     CDS             complement(5102..6148)
                     /gene="holA"
                     /locus_tag="IWI57_001947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBC2450083.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(6186..8459)
                     /locus_tag="IWI57_001948"
     CDS             complement(6186..8459)
                     /locus_tag="IWI57_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBC2450084.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(8452..8991)
                     /locus_tag="IWI57_001949"
     CDS             complement(8452..8991)
                     /locus_tag="IWI57_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBC2450085.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(9127..9345)
                     /locus_tag="IWI57_001950"
     CDS             complement(9127..9345)
                     /locus_tag="IWI57_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBC2450086.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(9342..9587)
                     /locus_tag="IWI57_001951"
     CDS             complement(9342..9587)
                     /locus_tag="IWI57_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBC2450087.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(9703..10431)
                     /locus_tag="IWI57_001952"
     CDS             complement(9703..10431)
                     /locus_tag="IWI57_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBC2450088.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         3206 a         2021 c         1846 g         3394 t
ORIGIN      
        1 acagattggg tttctcaaaa aataacggta tactataaga tgtatcttaa ttgggaagga
       61 aggaatttat gttttttgca aatattatca aagcagtgat tctcggtatt attgaaggaa
      121 taacggaatg gctgcccatc agtagtactg gtcatttgat cctagcagat gaattcatca
      181 aactggatgc cagttcacaa tttatgtcga tgttcaatgt cgttatccaa ttaggggcga
      241 tcttggctgt cgtcgttctt tattttcaca agttaaaccc attttctcca agtaaatcaa
      301 cggtagaaaa aaaagatacg tggacacttt ggtttaaagt gatcgttgct tgcataccag
      361 ccatcatcat tggtttgcct ttggatgatt ggttagaagc aaatttccac aagttcttac
      421 cagttgccat tgttttgatc gtttacggta ttgcctttat tattgtcgaa aaacgcaaca
      481 aaaacaaaac acctcgttgg acttctctga atgactttac gtaccaagca gctcttcttg
      541 ttgggttatt ccaagtcttg gcacttgttc ccggaacatc ccgttctggc gcaacgatcc
      601 taggatctat cttgattggt gcttcgcgtt ttgtcgctac agagttttca tttttcttag
      661 ggattcctgt catgtttggc gcaagcggct tgaagatcgt caaatattta gctgaaggga
      721 attcattcaa aatggaagaa actgttgttc ttcttgtagg aacattcgta tcttttgtgg
      781 tttccgtctt agctatcaaa ttcttactaa gctatttgaa gaaaaacgac tttaccgctt
      841 ttggctggta tcgtattatc cttggtatca ttttgattgg ttattggttt attgcaatgt
      901 agaaaaaacc agaacacttc tcttatttga gaaatgttct ggttttttat tttctttaat
      961 aataatcatt ataaataata aaaacaacat caagcttaat caccaaatga taaccattat
     1021 cgttctcatt tagctgttat tattatcaat tacattttta ttatgctatc cttgttttga
     1081 caaggaggtc taataacgat gactaatcag aaaaaagctt ttgcagcaac aatcggctgc
     1141 cttttattta tgatcttcgg ttttatcttc gaccgattct ctcttaccat caccccactt
     1201 ttttatatca gtgccattat catcggtgga tataaacaaa catcggaagg cttgaaagaa
     1261 ttgtggaatg atcggacact gaatgtggat ttattgatgg ctttagctgc tattggtgcc
     1321 tgtttgatcg gtaattactt tgaaggagca atgttgacat tcatcttttg tttaagcggt
     1381 gcattagagg aatacacaac taataaaagt caaaaagaaa ttactgcttt gatgaatctt
     1441 caaccacaaa aagcccaatt attaaaagaa aatggacaaa tgcaagaagt agatgtttct
     1501 caactaaagg tggataactt ggtatttgtc gctaaaggag cagctgttcc gatagatggc
     1561 tttttagaaa gcacacaggc aacgatcgat gagtctgcat taagcggtga atctgtgcct
     1621 gtagaaaaat atttaggcga tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc
     1681 acgattcgag taacaaaaac gagttccgaa actgtctttg ctaagattat ccaattagta
     1741 gaagaagcgc agaatacgcc aactcaaaca gcttcattta tcgaacgaat cgaaaataat
     1801 tatgtaaaat tgattttgct tgctgttcct ctaatgatcc ttctccccca ctttttccta
     1861 ggttggagtt gggatgaaag cttttatcga ggaatggtat tattagttgt ggcttctcct
     1921 tgtgcgcttg tcgcatctgc tacaccagct tctctagctg ctctttcaaa ttctgcaaaa
     1981 aatgggattt taatcaaagg cggtatccat ttagaaaagc tttctgaatt aaaagcgatt
     2041 gcctttgata aaacaggaac gctcactaaa ggaaaaccag ttgtaactga cagctttttc
     2101 tttgatgaaa aggatttggc acaacaactt cttgttgcta tggaacaaaa aacgacgcat
     2161 ccacttgctc atgcgatttt gcaatatttc gattgcacaa tacctgaaga aatccaatac
     2221 ttaccaatag aagaaattac tggttttgga ttacaaacta cctatttagg tgcgcaatgg
     2281 aaagttggca agcacgcata tgatcctgag acgatgataa tcagcaaaga gattgcggaa
     2341 atgatcgaac gtttggaaaa tcaaggcaaa accgtgattt atctttctaa agatcagcag
     2401 ttaattgctg ttttaggtct gctagatatt ccaaaagcaa atacccaaca agtaatttcc
     2461 tactttaaat cccaaaacat ccatacaagt atgataaccg gcgaccattc tggcacagca
     2521 aaggccattg ctgaacaagt agggattgaa cattactatg caagctgtac tccggaagaa
     2581 aaaacacaac tagtcaaaaa agagaatgaa ctttaccaag taaatgcgat ggttggtgat
     2641 ggagtcaatg atgcgcctgc tttagcagcc gcttctttag gtgtcgctat ggggcaaggg
     2701 acagatgccg ctatggatat cgcagacatc gtcttaatga aaagcgactt aaataaattt
     2761 gtctacagtc atcggctatc tttaaaaatg cggcggatca tcaaacaaaa tattattttt
     2821 tcgattcttg ttattctctt gttgattgct tctaacttcc tgcaattttt aaatctacct
     2881 cttggagttg taggacacga aggaagcaca atattggtta ttctgaatgg cttgcgttta
     2941 ctaagacctt tacctgatct ttctgattct tcaaaaaaat gtaagtcttg tccactatac
     3001 aaagcgcggc tgtcttctgt caaataagca aagtgttttt ttatgatttt gtttatttta
     3061 tgaaaaaaac agcttatgaa taggtcttga aggatgaatt cctacttttg taactttcta
     3121 aaaacatgtt atgattgttt cgaagaaagt gaggaatata tttcatggaa agttggcttt
     3181 ttttaggaat tattttactt gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg
     3241 cagcttttgt tttgattatg aagctcatac cagcctcccg ttcctttttc ccttggcttc
     3301 aaggcaaagg aatcaatatt ggggtgacga tcatcacagc ggctattcta atccctatcg
     3361 caacaggaga aatcggtcta cgtgatctat tagaagcctt taaatcccct attggctggg
     3421 tagctgtttt ttgcggtggg ctagtggctg tcttatcttc caagggtgta ggattgattg
     3481 cttctgatcc acaaatcact gttgcacttg ttttcggcac gattttaggt gttgtcttct
     3541 taagaggaat cgccgccgga ccggtcattg cttctggtat cacttactgt atcttacaag
     3601 tatttgcttt aggaaaatag aacaaatttt gtctatttta ggagattctt aacaccccca
     3661 atttactgat ccgtgatata ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat
     3721 ttcataggaa caatcggctg gtttcttcta gcggtacttt tgattctggg cttagcttat
     3781 ctattatttc ctgatttgat acaaaatgtg tttgatactg ctttttgatt gacctattaa
     3841 aacgatatag gggatgctcc tgcgatgcag gggctttttt taatcaatgt tttgtaataa
     3901 tttaggccgt gccaatgaga cttcctatag aattcgatgc tcttttttct ttttggctct
     3961 tctgctcttt tttgttatac tagattacag tggacaaaag aaagaatgat cgcatccact
     4021 tcaacgaata tcaaaatgga agaattagtg cctgttttca ggttactttg aaaagaaagg
     4081 aagacgcttg tggaaacttt tgaaggtctt ttaattgata cgtacgtttt acaagaacag
     4141 ccaatgatgc tgactgggat caacttgcag cttgaggacc gtgtgatcaa tggcctgctt
     4201 aaagatcgct atttggcaaa atatatctta cgtttgcccc taaataaata tgtgctggaa
     4261 gtaagtggca aatggaaccg tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat
     4321 atcgatgaat acattaaaat aatcggtaca ccagaatagc ctttactgat caaaggatac
     4381 tggttataaa ataactgcag tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc
     4441 ccttttcaaa aaaaactttg caaacggttc tctttgttat ataataactt agtaccaaac
     4501 attcccattc aaactttgtt taaatagata aataaaaatc tagtttggtt tgacgctatt
     4561 gcctcccaca atagcgtctt tttgattttt ttcagcttgt actatccgtt tttactgaaa
     4621 aaaaatccta ataaaagact gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac
     4681 gctgccttta ggtataataa tgaatgttga attatgcggt aacacgcgta agaacttaag
     4741 ttatcggagg tgaaactaat gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc
     4801 gaaaacgcta atgtgaaaaa ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa
     4861 tttgaagatg ctgtagcttc aggtgctgac aacgtagatg cgttatataa agaagctgtt
     4921 aaagctattg atatggctga atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac
     4981 aaatctcgtt taagcaaaaa aatcgctaaa taaaagaatc ggcttcggcc gattttttta
     5041 tttgtcaaaa aaaggacaaa aacaaacgac cttcccgcat gctttgccct cgtctttctt
     5101 cttatcgatt tcttaacaac tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt
     5161 tttcgatttg tcccgttttg atttgataat ccatctcaac taattgatca aacaactgta
     5221 ccagcaaatg ttcatcgaat ttttttactt cctgcatggc aagttttaca cgatatggat
     5281 ggattttcaa tgtttcagca atatttgcct gctgataacc aatctttaca agaaattttg
     5341 tctgtaaaaa taaacgtaat tgagaaagca aaatcgcatt aattttgatt gtttcttccc
     5401 cttgcataac taaatcttcg tataaacgca aagcttgttc cgtcttccct tttaaaatat
     5461 attgggtcat gtcaaaaata ttatgttcta acgttttagg aactaattct tctacttctc
     5521 ttaccgtgat ttgctgttga ttttgtccat aaaggaccaa tttctgtaac tctctcatca
     5581 ttttggataa atctaagtcg gtcaacctca agaataaatc aattgcactt cgttccattt
     5641 tgattgagga attctctaag gtattcatca gatattgacg tacttctttt tcattcaact
     5701 gttggacatt gatgataatt gctgtttttt tcaattgttt cgttactttt ttccgttcat
     5761 ccaacttttc atatggcgca aagaatacta atacagtcga ttctaatggt tctttcaaat
     5821 aagccgtcaa taaatcagta ttttgttcga ttccattgtt tactttttca cctgttaaaa
     5881 aataaggatt ctctacgaat actaaccgtt gatctccgaa aaacggcaac gtctctgctt
     5941 cagctactac ctcatctaaa cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat
     6001 cttcgccctc taattggatt tttttgacaa tttctgtccg aacttgatct tggagaaact
     6061 tttctgttcc taaaatcaag taacaagatg caagtttttc tttacgtatt ttttgtaact
     6121 ctgcttgtat actcaaatta ctcaccactt tcaaaaatta tcctaccatg agcagatgag
     6181 aattactagt ctatcattgt ttccactagc ccatgaggat gaaaaactgg atgccagcga
     6241 taggtgatca ttccttgttg atccgttcgc catgttttta tctgattttc ttctagtgtc
     6301 tccaatactt cgctgtgcgg gtgaccataa cgattattta cgccgcagga tataatccca
     6361 tgttttgctt caatatggga aagaaaatct gtgttacttg acgtacggct accgtgatga
     6421 cctaatttga ctacatcggc tttcagatta ggataaatca aaagcaattg tttttccatt
     6481 gcttgattca aatctcctaa aaataaaaag cgcctattgt tatattccat ccatagacca
     6541 atcgaatctt catttcttcc ctctcccttt tcaaaaggag tcaatacata aagatcataa
     6601 taaccgctga tcctgtcccc atgtttgatt tcaatgatct cagtaccatt tttttgagct
     6661 tcagataaat gcctttgtaa tggctcattt cgtccagcgc cccaaccgat ataaattttt
     6721 ttgatagtaa aatgctgaga taacgtgggt aattctccca catgatccgt atcgtcgtgt
     6781 gtcaagatga gtttatcgat tacacttatc ccctgacctt ttagatatgg aacgatattt
     6841 ctttcagcag gaggacgtat cataccttgc gcccactttt ctctttcaaa gtgtaactga
     6901 cctccagtat cgatcaaaat cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct
     6961 ccttgaccga catcgataaa agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa
     7021 aaaataaaag aaaaaagacc aacataaaac aagtgtttca caggttgttc tttcaaccta
     7081 tccatacaaa tcagtaatcc gaaaatatac atcccagata gcacaagggg caaacgtcct
     7141 ataatgagag acgaccctga agaaaaatcg attattcgct caaataagat taatccctct
     7201 tctatcagca aaagtaaagt ttccggcagc cagcgattga agagtgtgag aaataacaca
     7261 ccaggaagaa aaagaaactt gaataccggt gcaatcagta atgtaaaaag actacctaaa
     7321 ataggccact cataaaaatg ccatacaatc aacggcatcg tgatcatagt aaacaggacg
     7381 tgagaggccg tttttttcaa ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc
     7441 atgaaaaaac taagctgtcc acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga
     7501 agccaaaaga caactgcaaa taagtccatg gtggaataat gcttattcag caatttgact
     7561 agcaatcgca ataaaaacag caaactagca cgcaaaacag aaatagaact tcctgcaata
     7621 gaaacataac agatagttag tagactcaac ggaataatac tgacgtgtag tggccatccg
     7681 atacgtcgaa ataaataata cagccaccct aaataaaatt ggatgtgtag acccgataga
     7741 ctgaacagat gcagcaaacc actctcccta aaaatttctt ctggttctct gaatcctgca
     7801 tctttatagc cccataataa agaattaata tatacacttg ttttttgcgg aaattcagat
     7861 tggacaaagg agataaaaga tcctcttttt ctctgaagcc agtttcgcca gtgattttgc
     7921 atggtgatct gttcaatctt ttgaatttgg aaccgtccag aaattctatg gttcctgaag
     7981 taagcttctt ggtcaaaatt ataaagattc cgcttttttg ctggagagat gaactcgcct
     8041 tcagcagtca attctattcc ctttcctcgg aagttaagcc atgttttcaa ttctgcttct
     8101 gtgtttactt gataagaaag ctggagcttt tgtttattct ctgataacct tccttccatt
     8161 gtcaaaaaat taccatttgc acgaatcgta tctgtaaata cagtaatttg cgcagatgtt
     8221 gttttttcct ctaaaaatga atactcttgt agcctataag aaatggatac cgttatgcta
     8281 cacaaaatac acaagtacgc gataaaacgt ccttctttta gcgaaaacca taaaaaaatt
     8341 ccggcaagaa gcaaacgata accccaattg tcagtgtaat acaacaaaca gataatcatc
     8401 gacgtaattg ccggaaaaat caaccgattt cgaacatgcc tttctaaaac gctagacatg
     8461 ttttcctgtt aattcatttt gaagttttga aaaatgctgt gagtctaaag caacttggtg
     8521 tacagataca cctaattgtt cgatcaacgc ttgggcataa ggatcatttc gatagtcatg
     8581 gagataattg atccctttca caccagcttg caacaaagcc tttgtacagg ccaaacatgg
     8641 aaaatgtgtg acatagattt ctgaaccttc cgtcgggata ccaagttttg cacactgtag
     8701 gagtgcattc atctctgcat ggatcgttcg caagcagtgt ccatccacga cgtaacaccc
     8761 ttcatctatg cagtggacat ctccactgac tgctccatta tagccgccag caatgatcct
     8821 tttttctcga acgagtgtcg ctcctacttc taaacgtgta cacgtactac gtaaagataa
     8881 cagtactgcc tgagccataa agtactggtc ccaaggaatg cgttcattat gtgacttatc
     8941 agtaactttt tctttattcg tatcgcatgc ttttgaagat ttttctaaca tgttttatat
     9001 tcctccgttt tttcaagttg attaaaaagt actcattgct taaacaaaat attctttaaa
     9061 tataacgaat catcctgtct gacaaaagat cctacccttc actgccgaaa tgaagtccgt
     9121 catccattag cccattataa aaattcttcg aatccatttg ttcaagaaat tccgtataaa
     9181 aatcgatact tcacaatcat ggcgttttgc tacttctgtg agaacagaga tatattcttt
     9241 gatcaacttg ttattccgat atttttgttt tgtctcatcg actgctggtg gcgtgataaa
     9301 gaaagcatga tcagaactat agtctttttt cagctgaact atcatccact gcagattttt
     9361 tggtacgttt ctttagaaag ttgtttatgc tctgctagat cattcgtccc aatgagaata
     9421 aatactttat ctgctgtata gctaagtaca tctttggcaa agcgttctcg tagagcttga
     9481 ctgttattgc cgctgacagc cgtattcata atatgaatcc cagatatttt attttggaga
     9541 tagacattca gcattggttt ttttaatgtt tctttgcgaa caatcaagct atttcctatc
     9601 caaaaatact acctcctcgt cctgccaagc tcttagaatt ggattattct tagtatccat
     9661 tagcgtccat ttattgctcg tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa
     9721 gttttttagt ttttcaactg tcttttcacc gataccagaa atctcctgca ggtcttccac
     9781 tttctggaag gatccgtttt cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc
     9841 gatcccagga atctgctgaa gttctgatgg agtggctgta ttgatattta ttttgggagg
     9901 ctcggaatct gctgtaggct gttttccttc cgaggatgcc aactgatcaa gatgttctgg
     9961 cagctcttca cctacagcag ggatatatag gacttgctga tcttctatct tcgcagaaaa
    10021 gttgattcga tcgctatccg cttcttctaa taatccacct gcacgcttta gtacatcata
    10081 gacccgttct tctgaagaaa aggaatagat accaggctcc ttcacacttc ctttgatatc
    10141 ggtgtacatg acctttggac gagcatcctc ttctacatcg tttccttcag ctgatggatc
    10201 ttctccactg gaactaaacg aagaaagtat ttcttttgca gcagtctctt ctgtttctct
    10261 ataaaatagc aaatttgata ataaaaaacc acttgcgata caaaagataa gaactatcat
    10321 agacatccaa atgatcaatt tgggtgaagc tagtattttc ttgctgattt tttctatcat
    10381 tgttcttttt tcaaagaatg accagtaaaa atcattcatt actcctttca tttaaaattg
    10441 aagaaacagt gagctctggc aaaatcg
//
LOCUS       DADXTG010000089        10419 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.090, whole genome shotgun sequence.
ACCESSION   DADXTG010000089 DADXTG010000000
VERSION     DADXTG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.090"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            139..408
                     /gene="rpsN"
                     /locus_tag="IWI57_001953"
     CDS             139..408
                     /gene="rpsN"
                     /locus_tag="IWI57_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBC2450089.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            423..602
                     /gene="rpmF"
                     /locus_tag="IWI57_001954"
     CDS             423..602
                     /gene="rpmF"
                     /locus_tag="IWI57_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBC2450090.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            634..1011
                     /locus_tag="IWI57_001955"
     CDS             634..1011
                     /locus_tag="IWI57_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450091.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1032..1181
                     /gene="rpmG"
                     /locus_tag="IWI57_001956"
     CDS             1032..1181
                     /gene="rpmG"
                     /locus_tag="IWI57_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBC2450092.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1205..2188
                     /locus_tag="IWI57_001957"
     CDS             1205..2188
                     /locus_tag="IWI57_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBC2450093.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2218..2340
                     /locus_tag="IWI57_001958"
     CDS             2218..2340
                     /locus_tag="IWI57_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBC2450094.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2358..3881
                     /locus_tag="IWI57_001959"
     CDS             2358..3881
                     /locus_tag="IWI57_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBC2450095.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3905..4144
                     /locus_tag="IWI57_001960"
     CDS             3905..4144
                     /locus_tag="IWI57_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450096.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4337..4798)
                     /locus_tag="IWI57_001961"
     CDS             complement(4337..4798)
                     /locus_tag="IWI57_001961"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2450097.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4993..5211)
                     /locus_tag="IWI57_001962"
     CDS             complement(4993..5211)
                     /locus_tag="IWI57_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450098.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5329..6945
                     /locus_tag="IWI57_001963"
     CDS             5329..6945
                     /locus_tag="IWI57_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBC2450099.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7186..8526)
                     /locus_tag="IWI57_001964"
                     /pseudo
     CDS             complement(7186..8526)
                     /locus_tag="IWI57_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8802..8957)
                     /locus_tag="IWI57_001965"
     CDS             complement(8802..8957)
                     /locus_tag="IWI57_001965"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBC2450100.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9253..9585)
                     /locus_tag="IWI57_001966"
     CDS             complement(9253..9585)
                     /locus_tag="IWI57_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2450101.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10099..10290)
                     /locus_tag="IWI57_001967"
     CDS             complement(10099..10290)
                     /locus_tag="IWI57_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450102.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         3462 a         1658 c         2042 g         3257 t
ORIGIN      
        1 agttatctat tcatatgagt tttatttcat gttatttatt gttgacaaga gcaactcttg
       61 ttggtaaagt atcagtgtca aaacgtaacg attacgattt gaggtgtcta aatcaaaata
      121 aaaggagtat aaaggtatat ggcaaaaaag tcgaaaattg ctaaagcaaa gaaacaaatg
      181 gcgatgattg aaaagtatgc agataaacgt caggaattaa aagccgcagg agatcgcacc
      241 gctctcgcta aattgccaag agattcaaat ccgaatcgtc tgaggcttcg tgatcagacg
      301 gatggtcgtc caagagggta tatgcgaaag ttcggtatgt cccgtattaa gtttagagaa
      361 cttgcccatc aagggttgat tcctggagta aaaaaagcaa gttggtaaaa ggaggaaata
      421 tcatggcagt accagcaaga aaaacatcta aggcgaagaa acggttaaga agaactcatc
      481 aaactgtagt aaaaccagaa atttcatttg acgaagcgaa tggtgattat agacgtagcc
      541 atcatgtatc tttaaaaggg tactataatg gaaagaaagt aattaaagga gcttcaaaat
      601 agtttaatat ataactttta ggagggaatc aaaatggaaa ttatttaccc acctttagtt
      661 gaagaaggat taaagtatta tcttgaaacg acccagcaat cgctagataa atgcactttt
      721 tatcgttcga tggtagagag aggaattatt actgaaacgg gtttaccgac ccaacaggca
      781 attgaaaatg gattagtcaa agattactat gaggaccaag gcttatcatt tgatgatttt
      841 ttaagaatct atccaatttt tgaagagtat gacgaggagt tatttcaatg tatcgatggt
      901 tattgggaga tacctgttga tatgaaagaa aatttagttt cacaattgga atctggcgag
      961 ttaacttttg aagatgcaca acaaattcaa gcatatcttg aagatcgata agtattgaag
     1021 gaggaagatt tatgcgtcaa aatattattt tggaatgtgt cgaaactgga gaacgtctgt
     1081 atctcaccag caaaaataaa cggaataacc ctgaacggtt agaattaaaa aagtattcgc
     1141 caaaattgcg cagacgggca atttttaaag aagtgaaata gctaagtggt aaaaaggtga
     1201 caaaatgggc ataccagtta caatcgtttc tgggttttta ggggctggaa aaacaacgct
     1261 aatcaatcaa gccttgcaag tctcacccta ccccagggaa gaaattatta taattgaaaa
     1321 tgagtttggt gaagttggga ttgatcacaa attattagtt catagcgagg aacgggtgtt
     1381 acaactaaat aatggatgta tgtgttgcag tcttcgcagt gatttattag caatattgat
     1441 ttctttactg gaaacaatag aagaacatca acaaccaatt tcacaaatca tcattgaaac
     1501 tacaggtata gcagatcccc aaccaatcat tcaaacactt ttaacagcgc ctcaactaag
     1561 aggaagagta tatatcgata gtttattgac tgttattgat agtgacagtt ttgaacttat
     1621 tgaaagagaa ccacaagccc ttaagcaact tgtcatggca gatcgcattt ttgtgtctag
     1681 aaaacaaaac ttgaatttat ctgcactaaa aaaattacag agagaaatca aaagtattaa
     1741 ccctttatcc gatattcgaa tgttcacaca tagtgaaacg attcaaaaaa aggattttta
     1801 taatttgaaa aaatttaatc atcccttagt ttttgaaagt gattcatcag aaaatcacta
     1861 taatggcaaa tactatcatc aacaccatca agtacatgag tttaaatcaa ttctcttaac
     1921 ttcagatcaa gacataaaaa aagatttttt actccaatgg attgactggc tgattttaac
     1981 caaccaaggg agcctgtatc gttttaaagg attgattgct ttacaagagc aggattttat
     2041 agttgccatg caagaggtaa acgaacaggt taattttcaa tacaccactc gacaaaaaga
     2101 ccaaaccact attattctaa tcggaaaaat gttggatgaa gaaaggatca aacagagttt
     2161 tgataaatta ctggcggaaa gcaaataatg ataagtagtg attgggggta atttctaatg
     2221 gagaaaacgg atatatctag tgcatatcga cgacttaaaa gcccgaatat taaaactaga
     2281 aaaagggcat taaaaattat caaagaacat aaacgaaata aaatgaagaa attagcataa
     2341 ttaaggaagg aaaaagaatg aagaaaatca gtgtaggact tattgggata gcagctctag
     2401 gattattggg agcttgctcc agcactaatg atgcaaaagt atcgaatgat aaagatggaa
     2461 aattagaaat tgtaacaaca ttctatccaa tgtatgattt caccaaaaac atcgtcggcg
     2521 atgaagcgaa cgtggattta atggttccag cgggatcaga acctcatgac tatgagccct
     2581 cagctaagga tatggcaaaa gctcatgatg ctgatgtttt tgtatatcat aacgaaaaca
     2641 tggaatcatg ggtaccaaaa gctaaagaaa gttggaagaa agctgggcct aatgtagtag
     2701 aaggaacaaa agacatgatt ttattgccag gtagtgaaga agaagatcat gatcacggag
     2761 aagaagatca ccatcatgaa ctagatcctc atacgtgggt ttcacccaaa atggcaatta
     2821 aggaagtttc aaatatcaag gatcagttag ttaaattgta tccgaaaaaa gcaaaagtat
     2881 ttgagacaaa tgctgaaaag tatttgacta aactgaaacg gttggatgct gactatacca
     2941 ctagtcttaa agaagcgaaa caaaaaagtt ttgtgacgca acacgcagcc tttggatatt
     3001 tagctttgga ttatggattg aaacaagttc ctattgctgg actaagtcct gaagaagagc
     3061 catcttctgg tcgattagct gaattaaaag agtatgttaa aaaaaataag atcaattata
     3121 tttactttga aaagaatgct aatgataaaa ttgcaaaaac tttggccaac gaagctggga
     3181 taaaattgga agtactgaat cctcttgaaa gtcttacaaa agaacaaatg gataatggtg
     3241 aagattatgt ttcagtaatg gaagacaatc taaaagcgtt agaaaaaaca actatggttg
     3301 ctggtgaaga ggtagttcca gaaaaagaag cgaaggatga aaagacagtt gctaatgggt
     3361 actttaaaga tgccgatgtg aaggatcgcg aattgagtga ttatacaggc gaatggcaat
     3421 ctgtttatcc gttgttaaaa gatggaattt tggatgaagt tttcgattac aaagcaaaaa
     3481 tcaataaaga catgacagct gccgagtaca aagattatta tactactggc tataagacag
     3541 atattgatac tatcaacatt aaagacaaca ccattgattt tgtagtcaac ggtgaacatc
     3601 atcaatacac atacaaatat gttggataca aaattttgaa ttatgaaaaa ggaaaccgtg
     3661 gtgttcgttt taattttgaa actgatgatg caggtgctgg tcgttttaaa tatattcaat
     3721 ttagtgatca tggaattgca ccaagtaagg cagagcattt ccatattttc tttggtggag
     3781 aaagtcaaga aaaactctat aatgaattgc ataattggcc tactttctat ccagcttcat
     3841 tgtctaaaca tgaaattgct caagaaatga tggctcatta aaattcaatt tttggaggaa
     3901 atacatggta agaaaagtat tattgtctat ggattccaag aatattgatg cttatcggcg
     3961 ttatcgattt gcaaagcaaa tgagattaaa tgggagtaca ttagctgata ttgctgcaac
     4021 tttacatgtt agtattgaca cagttcatag agtattaaaa cgggatcctg attgttgttt
     4081 tatcaaggtc aatactggaa gaagtagacg tttagcaaaa cggcataata gcttggccaa
     4141 ttaattgata ataccagagt agatcatttt tcccttattt ggatttgatc tgctttttat
     4201 tatgtcttga attgtcaaat caagcgcaac actttatcaa agaagacttc ataaggtgtt
     4261 ctccaatcta aacactttct aggtctgtta ttcagttttt caatcatctg atttatcgtt
     4321 gagttatcta ccgtagctat ctctttcgat tttggcaagt attctcgaat gagtctattc
     4381 gtattttaat ttgttcattg ttgccaaggg catgaggatc tggaaaataa aagggcatat
     4441 tattcaatgc ttctgtaact gcgaaatgat tggagaattc ttttcctcta tctggagtga
     4501 tcgttttgac cgcctcattt ggaatactgc ttaaaagttc aatcattttc tcgctcacat
     4561 aagcggcttt ctttctgggg accttctttc ctgctaaaag gaagcatgat tttctgtctg
     4621 tcaaagtcac taaacaagta ccaccagttt tgccagctac aatatcggct ttccaatgac
     4681 caaaatcact tctatcatct gcggattttg gacggccatg gatcgaatga gtaaggcaaa
     4741 tctttccacg agactcgaca tgattttttg tatgtcttgt ttttcctcga tggtgcaatg
     4801 aacggatcag gcctttattg ccgtgtgaga ggggttctgt atcgaaaaga tcattgcaaa
     4861 ttgcacgtta gatcgtgata aaactaattg aaaagtccat atgttcatgc ttcaaacgat
     4921 tcgagatttc ctctggcgac cactgaaaat cgacaaataa atgacgaaca attgaccaaa
     4981 ctcacggatc atttagaatg tgataacgtc cgcatttttt tcttcgataa gcataatttt
     5041 tttccgcttt ggaaggtgaa tagttttttt cttgtttctt ctcagttcat gtaaaatagt
     5101 aaacgggcta tgctgaatgt cgttgccaat tatactaata gaaaatcctt cttcgtgaag
     5161 cagaaagatt ctttctcgtt cgagtatagt aagatgacga tactggctca taattatttt
     5221 ccttctagga gtgttctcga caaacttata ctagttggat atcgattatg actcattttt
     5281 atgtgttgca cttaaagtgt aaattcaagg tatagaaaga ggttccctat gaaaaaaata
     5341 cctattacaa tcttaactgg ctatcttggt tcaggaaaaa cgatacttct aaatcatatt
     5401 ctgtacggtg atcacggaaa ggaaattgct gtcattgtaa atgacatagg ggaagtaaat
     5461 gttgatgggg tattagtcga caagaaaggt tttaaaagaa ctggagaaaa acttgtttcg
     5521 atgccgaatg gatgcatttg ttgtacatta cgcgaagatt tgattgttga actagatcaa
     5581 ttagtgcaat tttctgactt gaatcaaatt gttattgaag cttcaggaat cagcaaacca
     5641 attccaatta ttcaagcaat tatattagct aaaaaccctt tagacattga tttgacccaa
     5701 cgattagaga ttggtgccat tattatagta gtggatgtaa aacgtttttg ggataatttc
     5761 gaaacgggtg aaaagattca ggaaagaaaa gctgaaggta atgtagttga agaagaacag
     5821 gatattcgtg gcttgttaat tgatcaattc gagtgttgta acatattact tttaaataat
     5881 agtgacttag tgtcaaccaa gacgatttac aaaattaacg gtctaattgc taagttacag
     5941 tccaatgcca aaataattag gacaattaaa ggtgaagtag acttatcgaa aattctaaat
     6001 acaaatcttt ttaatataga tgagacttcc tattcagtgg gctggttcaa agagttagag
     6061 cttggctatc aaaatcatat tcctgaaaca gaagaatatg gtatctcaag ttttatctat
     6121 cgaaatcggt tactccttga cgcacaaaaa tttgcccatc ttacggataa aaattttcct
     6181 ggcaatatta caagagcaaa agggtttctt tggtttgccc aacatagtga tttttcgacg
     6241 atctttgaaa catcgggtag ctataaaagt attgaatcat taaattattg ggtagctagt
     6301 ttacctaaaa ttgagtgtca aatgattata caaaaaaatc ccgaattgaa ggaagcctgg
     6361 catccgttgt ttggtgaacg cattaataaa ctagttttta ttggtatgaa gatggatcaa
     6421 caatgtatta aggaacaact tgagggttgt ttaactgatt ctcttaatgc agataatttc
     6481 cgaaaaattt caccatttcc atggaaaaag tctaatatgt ttagccagat atcgttaatg
     6541 aaatttttaa gaaaagctgg tgtcaagata ggcgatgtca taattattgg cattttgatt
     6601 attacctctt ttattccctt atttgtattc tctatagaaa aggagaatga aacaactgaa
     6661 gatgctatca aatatgccat cgttcgaatt gatggcaagg aaatggatcg cttcgatttg
     6721 gataagatca atcataaact cgtgaattat catcctgcga agggacaata taatattgtt
     6781 gagattaaag atggaagaat tcgagttaaa gaagataata gtcctgatca aatcgcggtt
     6841 agaactggat ggatctcaaa acccggtcaa acgagtattt gtttacccca taagttagtt
     6901 attagtattg agaaaaaaga aagcacagat tactacattt actaatctgg aaatagatga
     6961 ggaggttttt aataagaaaa agatattatt attaataata gcaggggctt tattaagtgt
     7021 gttaagcgct tgtggacaag aaaaagaagc ggtgacaaca ggcacaagta tctttaacgg
     7081 gacctgcaat ggtttagtta acagtcggtt agattactta ttttggctct tcgtcaatga
     7141 gtgttggtga agaaattcaa tagaataatc gagatactca cacggctaga cagctatctt
     7201 gtctagccgt gttgttgttc tggctttagt cagttgggta gtattcttag ttttcttaaa
     7261 atattttcct cgatataaaa agattcgttc tcggtagttt tgaaagtttc tatagccata
     7321 ggcaattcgt tttagtactt tgatatggtt attcaaacat tctatagggc cgttggaata
     7381 gggaacgcgc aacgctcgtt tgatttctgt ttggtatttc ttgaaggtcg tgaacactgg
     7441 ttggtagtgc tctggcaact ccttgtagtc ttcttttagg agcgcgacga attcaggaac
     7501 atcttgtctt ctgataacgg aaagaaaatc ctggtagact tcgtagcctc gctttaagga
     7561 atcatcataa gataatagcc gatcgactat ctctgcttct gtcagatgat ctcgaaatga
     7621 cggacgccag tagcgatgct gacctttcaa ttcccacgca ttcttttgaa gtagtttcca
     7681 gtatttcttt aagtgcttcg accgacgatc ccctttttta aaggtcttca ttacttgaat
     7741 tcggtggttg gtaaaagccc gaccaatgtg ttggctaata tgaaagcgat cggcgacgat
     7801 tcgggcattc ggaaaacact ctttcaccaa tgtgcgatag ggtgtataga aatctgaaac
     7861 cactagacgc acttgttcac ggacttttat agggtagcgc aagaaatagt cgcgtaaagg
     7921 attgagttgc cgattttcca caatatcaat cagttgatgg gtcttgccgt ccatcatgac
     7981 aaagctcatg gctcccgaga catttttcac tgatttgaat tcatcgaaac acaccacttc
     8041 tggtaacagc tggctcaaag aaatggtttt aggccgaagg ctgcgaagta ttcgaatgac
     8101 agtggtcgga gaaatgtgct tcatacgtgc gatcaaagac atagaaagag gttcgcttaa
     8161 tagctcaaga atggcttgtt tgactctgcg agcaatggag cagcgacgat cactaactga
     8221 atcttctgct aaaaaagtct ttccacagtg tttacatttg aagcgctgtt tgttaatccg
     8281 cagaatggtc ttgtagttac tgacatcatt caataagatg gatacttttt tccagcccta
     8341 tttaatgatc gtttgttcat tttgatagtg gcgatgaaag cattcctccg gttgataggt
     8401 caatgtgcca tcaaaaacaa aacatgtttc gtcattgatt cgctctttgc gaaaggcatc
     8461 ttcatgaaag ataagattta ggtctagaat atctagtaac tctttggtaa actctgaata
     8521 ggacatcttg aaacactcct ttgaatggtt ttcgtcgact ttattctaaa ggattttaag
     8581 gtgtctgttt gcgtttatta gaaaaaagaa aaagtattgg caacagaagg cgtcaccaac
     8641 actagatatt atagatccct tattttcttc gcttcaaatt gtcaaggagt catcataaat
     8701 tttgtatgga ttcttttgtt tgggtagtag tcttctaaat aatcttggat taacaccttc
     8761 atctctattt tacttttata tttgttggag tcgattaatt ctcatttgat tgatcgatga
     8821 aacgcttcaa ttactgcatt gtcaacagga gtcccagttt ggctcactga atgtgtgatg
     8881 tggtggtcgt tcaacagctc ttgataaata aaagaccgat gctggctacc tctatcggaa
     8941 tgaattaccg taggcaattc tccttaataa atttgtaatg gacggacgac aatctctttt
     9001 ttcatgtgtg aatctaccat atagtaacgt agaatacgag ttgctaagtc aatataggtg
     9061 gacaaataga ggcgaccgtc attacatgga atgtaagtaa tatccatcac ccaaacagca
     9121 tcaatttggt tctgttcaaa ttatcgattc aacatttttt taccatttct ttgattgttt
     9181 tttctctacc atgagaagat ttgcgtcgat gaaatcctct tgtgcataaa ttcatttcac
     9241 gcataagaac agctacaaca cgtttattga tttcgattcc ttcatcgtaa agtttttgag
     9301 tgatccctgg agatccgtat cgatctttgt gttcgtagaa cactcgtctt cctcttttag
     9361 tcgtttattt tcctgttcaa gaatgtagaa atctgcttta tcaggtaatc tttttccttg
     9421 tccagcaaaa gcatttttcc cgtatcgacg atattctgaa acccatcggg aaattgttgg
     9481 acgagagata ttgtattctt cagcaatttt cttggtagtt acttttttct caaggatatc
     9541 ctgtgaaatc tttagtttaa atttacgatc atatgttttt ctcaatagag ttcattcctt
     9601 ccggtaacgt aactgatagt caagaaaagt gtatcatagc ccaacttagt atcagggttt
     9661 gtcaactaaa ctgtggaagt tgatagataa gagtttttaa caaccaaaat tctcttgact
     9721 atttttgaaa tcagacaaat tttgattgga cacatagttg aataaatcta ccgtcgttac
     9781 ggaaggtaaa tcgcatgctt ttcaattcta gaggagaagt cctcgttgaa gtaccatagc
     9841 ccagttctgg attcgacctc ctgcccactt catacgtaaa taaagtaatt ttaggagtga
     9901 gactgtgaca aaagttattt ttaagctaaa aacgaattaa aaaacgttga taattaaaat
     9961 cattcgtttt agattctatg aatcattata gaacgctgta aaatcgatac tttataatta
    10021 taaagaagat agtttcaaat ttattgttcg aaatttgaaa ctatcttttt acttatgtca
    10081 cagactccct tagttgagtt attctttaat ttcgaatttc gagatagcta gatatattgc
    10141 aagcatacat tcttgataac aatcttgatc atattttcca gttctccagg attgcttatg
    10201 aatcaaagag ctaaatcttt tgagaattgc ctcaacagct tcttcatttc cagcctttgc
    10261 taaaatgaaa tcttctttta aagaattcat aaatctacct tcttcccatg gacttataat
    10321 ctatttcttt gggaaggagg agaacgagca aagaagcata caaaattagt tattatgcag
    10381 atattaaacc agcatttttt gaattttttt gtaaagtcg
//
LOCUS       DADXTG010000090        10177 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.091, whole genome shotgun sequence.
ACCESSION   DADXTG010000090 DADXTG010000000
VERSION     DADXTG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.091"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(640..864)
                     /locus_tag="IWI57_001968"
     CDS             complement(640..864)
                     /locus_tag="IWI57_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450103.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            1055..3832
                     /locus_tag="IWI57_001969"
     CDS             1055..3832
                     /locus_tag="IWI57_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBC2450104.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3914..4333
                     /locus_tag="IWI57_001970"
     CDS             3914..4333
                     /locus_tag="IWI57_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBC2450105.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4345..4815
                     /locus_tag="IWI57_001971"
     CDS             4345..4815
                     /locus_tag="IWI57_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2450106.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4832..5611
                     /locus_tag="IWI57_001972"
     CDS             4832..5611
                     /locus_tag="IWI57_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2450107.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5598..6419
                     /locus_tag="IWI57_001973"
     CDS             5598..6419
                     /locus_tag="IWI57_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBC2450108.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6436..7485
                     /locus_tag="IWI57_001974"
     CDS             6436..7485
                     /locus_tag="IWI57_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2450109.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7498..8502
                     /locus_tag="IWI57_001975"
     CDS             7498..8502
                     /locus_tag="IWI57_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2450110.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9135..10154
                     /locus_tag="IWI57_001976"
     CDS             9135..10154
                     /locus_tag="IWI57_001976"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBC2450111.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3421 a         1670 c         2109 g         2977 t
ORIGIN      
        1 tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt cattcatagg
       61 aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata tatcaagtaa
      121 atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa tcttttttgc
      181 tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca aagaaggagt
      241 atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa
      301 tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc
      361 tcccgacatc aagtcaaaag ctacttgaga ttttgatctg aaaataaaaa aagaaaacaa
      421 ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg
      481 tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca
      541 taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta
      601 atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt
      661 atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt
      721 atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac
      781 aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct
      841 gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg
      901 aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa ttataaggga
      961 taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa gattttattg
     1021 acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga agaaggaaat
     1081 tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga acgaggtctt
     1141 tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca gtcattattt
     1201 gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag tgatttattt
     1261 ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt atttttctat
     1321 cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat tctcttttat
     1381 gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag ctctttacta
     1441 tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta aaagttattt
     1501 agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt ctgcaccatt
     1561 catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta gcaatttgtt
     1621 tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg cttttgaagc
     1681 agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag aaggtcagga
     1741 aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac ctaatttgtc
     1801 acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta gtcatttctt
     1861 aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta atcagcgtag
     1921 tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa gaaaaatggg
     1981 gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga aattatccgg
     2041 aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat atgctgaaca
     2101 gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac tacttgcgca
     2161 gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc agaccttaga
     2221 gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt ttgagcaaat
     2281 cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa aattaaagca
     2341 agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag aaaaacacga
     2401 gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg aaactgccta
     2461 tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct atcagcgaag
     2521 cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag aattagaaca
     2581 acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc tggaattaag
     2641 taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga cgatctatct
     2701 aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg ctgctcacgg
     2761 ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag aggatctatt
     2821 tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag aaatccttga
     2881 ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca tgggatctct
     2941 gaaagaaatc catcagtttt ttaaaaaaca gttatccgta ccgcttttga ttttgaataa
     3001 tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg tatcattaga
     3061 agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc tctatccaga
     3121 ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta cggcggtcca
     3181 cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa tcatcccata
     3241 tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca tatacgaagt
     3301 cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc tttcattaga
     3361 agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca ctactatgaa
     3421 tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat tggaaaaagt
     3481 actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg atcattttat
     3541 gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat tagcgttgta
     3601 tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg aaacctatca
     3661 aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta aatctgcgtt
     3721 tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg cctatatcta
     3781 tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt gaacataagc
     3841 aagttgttgc cactaatttt ggcatgcaac ttgctttttt tagtatgtgt aagtgaaaag
     3901 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
     3961 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
     4021 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
     4081 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
     4141 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
     4201 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
     4261 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
     4321 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
     4381 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
     4441 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
     4501 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
     4561 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
     4621 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
     4681 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
     4741 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
     4801 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
     4861 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
     4921 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
     4981 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
     5041 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
     5101 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
     5161 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
     5221 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
     5281 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
     5341 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
     5401 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
     5461 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
     5521 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
     5581 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
     5641 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
     5701 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
     5761 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
     5821 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
     5881 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
     5941 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
     6001 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
     6061 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
     6121 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
     6181 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
     6241 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
     6301 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
     6361 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
     6421 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
     6481 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
     6541 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
     6601 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
     6661 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
     6721 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
     6781 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
     6841 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
     6901 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
     6961 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
     7021 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
     7081 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
     7141 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
     7201 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
     7261 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
     7321 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
     7381 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
     7441 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
     7501 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
     7561 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
     7621 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
     7681 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
     7741 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
     7801 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
     7861 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
     7921 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
     7981 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
     8041 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
     8101 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
     8161 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
     8221 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
     8281 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
     8341 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
     8401 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
     8461 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
     8521 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
     8581 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
     8641 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
     8701 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
     8761 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
     8821 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
     8881 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
     8941 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
     9001 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
     9061 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
     9121 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
     9181 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
     9241 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
     9301 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
     9361 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
     9421 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
     9481 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
     9541 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
     9601 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
     9661 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
     9721 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
     9781 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
     9841 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
     9901 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
     9961 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    10021 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    10081 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    10141 tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DADXTG010000091        10165 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.092, whole genome shotgun sequence.
ACCESSION   DADXTG010000091 DADXTG010000000
VERSION     DADXTG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.092"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            57..1481
                     /locus_tag="IWI57_001977"
     CDS             57..1481
                     /locus_tag="IWI57_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2450112.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1780..2487
                     /locus_tag="IWI57_001978"
     CDS             1780..2487
                     /locus_tag="IWI57_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBC2450113.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2474..2797
                     /locus_tag="IWI57_001979"
     CDS             2474..2797
                     /locus_tag="IWI57_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBC2450114.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2886..3923)
                     /locus_tag="IWI57_001980"
     CDS             complement(2886..3923)
                     /locus_tag="IWI57_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBC2450115.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4244..5227)
                     /locus_tag="IWI57_001981"
     CDS             complement(4244..5227)
                     /locus_tag="IWI57_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBC2450116.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5642..6412)
                     /locus_tag="IWI57_001982"
     CDS             complement(5642..6412)
                     /locus_tag="IWI57_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2450117.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6405..7340)
                     /locus_tag="IWI57_001983"
     CDS             complement(6405..7340)
                     /locus_tag="IWI57_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450118.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7656..9305)
                     /locus_tag="IWI57_001984"
     CDS             complement(7656..9305)
                     /locus_tag="IWI57_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBC2450119.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9422..>10165)
                     /locus_tag="IWI57_001985"
     CDS             complement(9422..>10165)
                     /locus_tag="IWI57_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBC2450120.1"
                     /translation="KSIAQIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAE
                     NDHYFTFYADGLKEYERIENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMME
                     TPLAQEIGEYKVTFGFNVEEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKI
                     WPLQNELDVPRKFSLIELPEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSL
                     GTVVNALENKQELTTIPPYDYLSILINER"
BASE COUNT         2985 a         2100 c         1840 g         3240 t
ORIGIN      
        1 ttactttaag ttgtaagcgg tgttatcatt aagtggaaac aggaggtaaa aaaattatga
       61 caactatgaa aaagctaact tttccagatg atttttggtg gggttccgct tggtcagctg
      121 agcaagcaga aggaaaagga gaaacaggaa aagcagagac tatctgggat cgctggttta
      181 aagaacaacc ttaccggttt tatcaacgaa tcgattcctc tattacaacc gatcacatcc
      241 atcaatataa agaagatata gaactaatga aaaaaactgg gcataactcc tttcgcgttt
      301 ctatttcttg ggcacgaatg tttccaaatg gcggtaccgg aaaagtaaat cccaaagcca
      361 tttctttcta tcgaaaatta ttttccgaaa tgaataataa cggaatcaaa gtatttgcca
      421 acttatatca ttttgacatg cctgctactc ttcaagataa aggaggatgg gaatcacgtg
      481 aagtagttga tgcttatgtt cattacgctg aaacttgttt taaagaattt ggtgacttag
      541 tttgtcattg gtttaccttc aatgaaccac ttgggcctat tttaggtact tatcttgaag
      601 attttcatta tccaaacctc attgacttta aacgcggagc gcaagcagca ttcttcacta
      661 tacttgcaca cgcaaaagca atacaagtat ttaaagaact agctcttcct agtaaaatag
      721 gcgttattct aaatttgagt cctacttttc ctcgtagtca aaatcctgct gatgtgaaag
      781 cagcagaaat cgctgattta ttttatacgc ggagcttttt agatcctatg gttaaaggga
      841 atttttctaa aaagcttatc ctgttattac aagaatataa tcagcttcct gaagattata
      901 cgaaagaaga tcttgtatgt atctacgaaa ataccgctca aattcttgga ctaaactatt
      961 acgagccacg tcgcgtcaaa tctcgttctt ctatggtgga tacatctagt ccatttttac
     1021 cagagtggtt tttcgaacca catatcatgc ctggacgacg aatgaacgaa taccggggat
     1081 gggaaattta cgaaaaagga atttatgatt tgtgcatgga tatcaagaac cattatggaa
     1141 acatcgaagc atttatctcg gagaacggaa tgggcgttgc ggatgaagaa cgtttcatgg
     1201 atgatacagg gcaaataatt gatgattatc ggattgagta tatcaaagat catcttgctt
     1261 acttatggaa ggcaataaat gaaggatgta acattaaggg gtatcatctt tggacattta
     1321 ttgattgctg gtcatggatc aacgcctata aaaaccgata tgggctggtt tcactcgacc
     1381 ttactactca aaaaagaaca attaaaaaaa gtggcgaatt ttacaagcaa ttaagtgata
     1441 caaatggatt tagatacgat gtagacttgt tagttgaata atattctaaa attccacttg
     1501 aagtgaacac attagaaaac aagaataatg agaaatacca ataacaaata ctttttcttc
     1561 atacttcatt tatacttgtc agtataagag actgtgacaa atgtagtgat cctcagaagt
     1621 taggtctgga aatctaactt gagaagattt ttttaaacta cgtccagtct tttttatttg
     1681 tttaaaattg aagttgacaa gtcttcatta aaaatttaga atattcacaa gtttgacgac
     1741 aattatgaaa atttatgtcg tgctaaagga gttttgttat tggatgaaaa gttagatata
     1801 aaaactgcga tcaaagacac gttacctacc gtttttggtt atatcggtat tggacttgca
     1861 tttggtatcg ttgggaaagc tgccgggttt catccgctgg tcgtcacgtt gatgtccctg
     1921 ctgatctatg ctggttctgc ccaatttatc acggtcagca tgcttgctag ccatagtcca
     1981 ctgctctcca tcgttctctc gacctttcta gtcaattccc gaatgattct gatgagtatg
     2041 acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa atctgctgat tggcacattg
     2101 ctgacagatg aaagtttcgc actgggaatg aataaattga actatactgg acaaaagtta
     2161 aactttcgtt ggatgaatac agctaattgg atttcttatt tgacgtgggt gggttcttct
     2221 cttgttggcg cacttttagg gaattttatt actgatccga aaaagtttgg tttggatttc
     2281 gccatcgtcg ctatgtttat cgggttactt tatcttcaag tcatttctga tagaaacacc
     2341 tctaaacgat tacagttgat cctgattggt ctcactttga ttctggtata tgtcggactg
     2401 atttttatcc caagtaactt agtgatttta gttgtaacac tgattgggtg cggattgggg
     2461 gtgtggatca aacatgcctt cttttgagtt tacattgctg actattttag gttgtacttt
     2521 agcaacttgg ctttctagga tcctaccatt tgtcttactg aaaaaattcg atttgccgca
     2581 accgttgctt gagtatttaa gttttgtccc gatcgtcatt atgtctgctt tatggttcag
     2641 cagtctattt acccaaaata tcggacatct gccgcagatc aatctcgaaa atgctttggc
     2701 ttcagttcct acacttttag ctgctattct ttcaaaaagc ttattggtga tcgtcttggt
     2761 aggaattctt tccctttcgt tgattcgctt attataaata aaaaaacaag agcttatggc
     2821 aaattactgt catgcgctct tgtttttatc aaagtcaaat aagaaaaaaa tcggcgtatt
     2881 cttgcttatc cgttgaacgg accactaaca acgacttttc ctaatatatg tcctgtttct
     2941 acgtctttgg ttgcttgttt caagttcttc gcattcatcc catctgaata gaccttgctc
     3001 aatgtactgc ggataatccc ttcatttgct aaagcagcaa tgtgcgctaa agcttctcct
     3061 tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca tatactccca gtcgaagctg
     3121 atagattggt ttttccaaac acctatatct aacggctctt tgatccctac gatcgttccc
     3181 acatgtccaa atggtcggac aaggtgtttg atttgatcaa agtagcgggt aatatcgaat
     3241 aatacagcga catactctac ttgatcgatt cctaattcac gaaaactttt acttaagtcg
     3301 tgatgataat cgattggata gtctacacct gtttttttca gccattcaaa attcttcgga
     3361 cttgcagtcg cgtagacttc taggccagcc caatgcgcta gttgattcaa aatcgaaccg
     3421 acgcccccag ctccgttgat cactaagatt tttttcccgc ggttggcatt ttcttctgga
     3481 gtcagaccaa atttttcaaa taacaattca taagcggtca atgaggtcaa cggcaaggct
     3541 gcggcttctt catctgaaag attttttggt gcaagagcga caatacgttc gtcaaccagt
     3601 tggtattctt gattgcttcc tgctcttgtc gttgtacctg cgtaaaaaac gcgatcgccg
     3661 acagaaaatt tttgcacttc atcacctaca gcaacaactt tcccgactcc atcaaaccct
     3721 aagatacgta aagcatttct tatcccgtta tcttggcgaa gtttcgtatc gacaggatta
     3781 acagatactg cgttgacttt taccagaaga tcccgtgaac tgggtacagg tatctgttct
     3841 ttttcatcaa taaagctttt tggatcatcg atcgacaacc cttcataaaa accgactacc
     3901 ttcattaatt ccttttctga catacgaatt cctccttagc ttcatcaagt tggagcatcc
     3961 atacagtttt catttcaata tatatagtat gctatttgtt ccacttttat tcatcataat
     4021 tattctctta cttaaagtaa actatttgca cttttttggt taaaaaagag gttgcgacaa
     4081 gacttttgtc acaacctctt ttaaacggtg ttcaatcaac tgaccctcgg tattcgctca
     4141 aaaaacgaca aaatatgagg agctattttt gtttggctgt tcttattact tgatgcagga
     4201 cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa
     4261 gcgttggatg ttctttttca aaggtgtcta agatctctcg tacttcatca gtagatttcg
     4321 tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa
     4381 agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat
     4441 ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc ggttcttttt
     4501 caaatgcata aggattcttg aaaatccccc ggccaatcat gatgccatct acgccatatt
     4561 tttcggccaa ttcttctcct gtctggcgat ctgggatatc tccgttgatc gtgatcagtg
     4621 tttgcggagc gatttggtca cgtaaagcaa tgattttagg gatgacttcc caatgtgcat
     4681 ctactttact catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat
     4741 gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct
     4801 tgacgctgac aggcaaaccg ccagctttag ccgcttcaat caattgtgcc gctacttctg
     4861 ggcgaaggat caagccactg ccttttccac gttctgctac attagggact gggcagccca
     4921 tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa
     4981 attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaaggtca
     5041 atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact
     5101 cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg
     5161 ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag
     5221 tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa
     5281 acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt
     5341 ctgattgagc aaaaaaagac tgtgacaaaa atcttatcac aattcctttt ccggaataaa
     5401 tggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aaacgaaaaa taggaggagc
     5461 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca acctctccac
     5521 taaacggtgt tcaatcagct gaccctcggt attagctcaa caaacgacaa aatatgagga
     5581 actattttcc cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt
     5641 tctaacttct caatcctctt cctttattga tcaaatacca agtaacggcg tacaaaacaa
     5701 cgatgaatag cacaagtacg atcatcgatg ttgctactgg cacatcgatt gttccaagga
     5761 atccgtaacg gaaaccggag atcatataaa cgatcggatt gactttcgaa acagcttgcc
     5821 aaaatggcgg caacatagag atggcataga aaacgccacc tagataagtt aacggctgca
     5881 agacaaatgt aggcacgatc gatacatcgt cataagactg agcaaaaatc ccgttgagca
     5941 gtcctgctaa cgaaaacaag attgccgtca tcaataatgt aatgatgacc atgaaccatg
     6001 aatacacatg aagtggtaca aagaacaagg aaatgatcgt tacgagagag ccgacgagaa
     6061 tactgcgacc cactccgcca atcacaaagc cccaaatgat cacatgcgta ggaacaggcg
     6121 ctaccaagat ttcttcgatg tttttctgga atttttgaga gaagaaggac gaagaaacgt
     6181 tggcatacga actagtgata gcagacatca taatcaaacc aggcacgatg aattccatat
     6241 atgaaaaacc acccatgtca ccgatccgac caccgatcat ttttccgaaa ataacaaagt
     6301 acaaggatgt cgtgatcaca ggcggaacca gtgtttggac ccagatacgc aaatagcggt
     6361 tcgtttcctt gatagctaaa ctttttaatg cagtaaaata aagattaaac atttttctcc
     6421 tccgtatgat tattttcttc tgtgattttc aaaaacagtt cttctaaacg attcgactta
     6481 ttcctcatag aaagaacttt gattccttgt cttgtcagct gatcgaagat tccgttcacc
     6541 ccttgatttc gttcaacttc gacagcgatt gtctgttcat cttcaaatgt acaagcgtat
     6601 ccttcaatca caggtttggt ctcatatggc gctaaatcaa agataaatgt ttcaaattgc
     6661 agtttggata gaagctcttt catactagta ttctcgatca gttcacctga ttggatgatc
     6721 ccgatgttgc gacaaagcat ttcagcttct tctaaataat gcgtcgtcaa aatgatcgtt
     6781 gtaccatttt cgttcaattc tcggagaaac tcccacatct cgcgtctcaa ttcaatatcg
     6841 actcctgctg ttggttcatc caagatcagc aaacgtggtt catgcatcaa tgccctagca
     6901 atcatcagtc ttcgcttcat tcccccggaa agcatacgtg cacggacatt tctcttttcc
     6961 caaagattgg actgcttcaa atatttttcg ctgcgtttca tcgcttcttt gcgagagaca
     7021 ccgtagtagc ctgcttgatt cacgacgatt tgctggaccg tttcaaaggg attgaaattg
     7081 aactcttgcg ggactaatcc aatctgttgc tttgctcgga ctagatccgt atccagatca
     7141 taaccgaaga cattcacttt tccagatgtt ttattgacta aagaagtaat gattccgatc
     7201 gttgtagact tcccagctcc atttggtcct aatagcgcat agaaatctcc ttctttcacg
     7261 gaaagatcga ttcctcgcaa tgcttcgacc ccacttccat aaaccttttt caaattattt
     7321 atttcaagcg catttgccat ttatttccta tctccttata gacatgattc tttattgtat
     7381 catggaatac agcagatgaa aaaacaactt ggttccagcg aaaaatcagg ttgctgggct
     7441 caggaaaatc tcctaatgaa agacatcaat cggacgaagt cagacaacct tttcagaaaa
     7501 aacaaatcga agataaagga ttactattca tatgtgcaac aaaaggagcg tgagacaaaa
     7561 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc aaaagccaag
     7621 atttttgatc caataaatac tttcgatcac ttcgcctatc ccaatacatg ccactgccgc
     7681 ttttcatcaa ataacgcggc ttccgttttt gcggtttcta ctaaagtctc tgggtaatcc
     7741 aaaactttca gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa
     7801 tcaaaactga ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa
     7861 ttctttaaaa tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac
     7921 tccgcaagcc accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgtccct
     7981 tttaaaatct catctacgaa gcagtagcag cgttctttgg aagccacttg gtccaacaaa
     8041 cgtttgatcg atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg
     8101 attgccatgg aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt
     8161 tggatcgtct gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct
     8221 gatgcattgg aacccgtgac taatgtattc ttctgccagt tgaccggatt cactaccgct
     8281 tcttttaaca atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt
     8341 cggcaagtga tgggcatata agtacggtaa ttcaatattg cagcggctac ttctaattct
     8401 cccagcaatt cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg
     8461 aaatgatagg agataaatgg cagcatgaac atgatgttga catattcaaa aatcaactct
     8521 gcttcactcc cactcttcat acgaaatgaa aaacctaatt tagggatgtc tcttaatggt
     8581 ttagtttgct gtttcagctc ttcttgaagt ggtgtcggca gttttgctag ctgattggcg
     8641 gctgaaatcg ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc
     8701 ttcactagat aatagaccgt attgaatact aacccaccga ttgccaaata gattcctagt
     8761 acttgtccta aaagaagcaa acccaaaccc accagcggaa gcaagccaag aaaaataaat
     8821 aaacctacgc gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta
     8881 tgatcttttt ttcctaaacg agcgaattga tattggattt tttctctcag ttgaggattt
     8941 tcctgataaa aagcaatcag tttttctaac tgctgatctt caccaaaatc aaagctgcgt
     9001 aaacgttgat acaacgcttc tgaacctaca ctagaatagg tactgttgat tccttcgaat
     9061 acttcgaaca tatccaggtc ataccaagta agatcatcga tttcgctatc ccatgaacgg
     9121 aacttcttct ccgtgagcca tgcttccttc aagctttctt ctttatcaaa tctcggttga
     9181 taaggaattt tttcccattt gctacgaact gtttccttta atttcaaacg tgaaaaaatg
     9241 tctaaagcaa ctaataaaat aacagctgca ataataatta aaacgataaa ttctctttga
     9301 cccatatccc tctccgttcc tttatttgtt agctttagta tagcactgtt gcgctatttg
     9361 aaaagagggt aaaaaaacac ctttcaaaat aaaaggtgtt tttttcttgt atattacata
     9421 cttatctctc atttatcaaa attgacaaat aatcataggg aggaattgtt gttaattctt
     9481 gtttattctc cagtgcattg acaactgtcc caagggaaag gatagcctca ggtactattt
     9541 tttcttgttc tgttggatta gctaagcgaa gaacaaatcc ttgttcatga aaataagaag
     9601 gataaatcga tgatacaatt aagtcttcag gtaattcaat caatgaaaat ttacgaggaa
     9661 catcaagttc attttgtaat ggccaaatct tgttatctaa cctgtgaata aatacattca
     9721 gtttctgctt ttgataagaa atagattgtt ctaaccgttt ttctgcctgt tttgccactt
     9781 tgaactcatt aaatcttcct tcttctacat taaatccaaa agtgacttta tactcaccta
     9841 tctcttgagc tagcggtgtt tccatcatga catgcccttc atttgtcgta tctcctgaag
     9901 ctcgcccagg tcgccaagca agattaggtt ttccaagttc ccctgttgtt gaaaataagg
     9961 ttatgattaa ttgattttct atacgttcat actcttttaa accatctgca taaaacgtaa
    10021 aataatgatc attttctgcc acagagacac tcttatcgaa tatttctaaa ttaacaggtt
    10081 tctctaccat agtttctgac caattcggag gaatgacatc aggggtattt tcaataaatc
    10141 cgttttgaat ttgtgcaatt gattt
//
LOCUS       DADXTG010000092        10121 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.093, whole genome shotgun sequence.
ACCESSION   DADXTG010000092 DADXTG010000000
VERSION     DADXTG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.093"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            105..611
                     /locus_tag="IWI57_001986"
     CDS             105..611
                     /locus_tag="IWI57_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBC2450121.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            628..1527
                     /locus_tag="IWI57_001987"
     CDS             628..1527
                     /locus_tag="IWI57_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBC2450122.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1614..2498
                     /locus_tag="IWI57_001988"
     CDS             1614..2498
                     /locus_tag="IWI57_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBC2450123.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2690..2761)
                     /locus_tag="IWI57_001989"
     tRNA            complement(2690..2761)
                     /locus_tag="IWI57_001989"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2726..2728),aa:Arg,seq:ccg)
     gene            2821..4155
                     /gene="rpoN"
                     /locus_tag="IWI57_001990"
     CDS             2821..4155
                     /gene="rpoN"
                     /locus_tag="IWI57_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBC2450124.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4304..5395
                     /locus_tag="IWI57_001991"
     CDS             4304..5395
                     /locus_tag="IWI57_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBC2450125.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5436..6437
                     /gene="gap"
                     /locus_tag="IWI57_001992"
     CDS             5436..6437
                     /gene="gap"
                     /locus_tag="IWI57_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBC2450126.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6547..7737
                     /locus_tag="IWI57_001993"
     CDS             6547..7737
                     /locus_tag="IWI57_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBC2450127.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7841..8596
                     /locus_tag="IWI57_001994"
     CDS             7841..8596
                     /locus_tag="IWI57_001994"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBC2450128.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8712..10010
                     /gene="eno"
                     /locus_tag="IWI57_001995"
     CDS             8712..10010
                     /gene="eno"
                     /locus_tag="IWI57_001995"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBC2450129.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3546 a         1864 c         2099 g         2612 t
ORIGIN      
        1 ttttttgtat ataaaaatcc caagtaaata cataaaaaat aaaaaaattg aaacttttag
       61 cgcaccttgt acgtcatatt aagtgaggca gaaaggagtg gagaatgatt tttactggat
      121 ataagaaaaa acggcagatc gaaaaatacc gtcacctgct gaatggactc atcgaaactg
      181 attatcaaaa aatgttccgt atcacgatga attacgtaca tgataaagaa gaggccttag
      241 atgtgatgca agatagtttt cataaagccc tgaactattt tgaaaaagaa aaagagatcg
      301 agcattttag tgcatggttt tatcggatat tgatccgcac ggcattagat agttggcgca
      361 gacaaaaaag gacgcctgtg gacttatttg aaattcaaga tatcccccaa cagttacctt
      421 tagaaacttc cgttgcagag ctccatatga ttttagataa aatagacagt ccggaaaaag
      481 agattctgat cctctacttt tttgaaggat ttcgtttgaa agaaattgcg actatcttgg
      541 atatgaatga aaacacagtt aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa
      601 tcctagaatg aaggagagaa gagtgccatg tattcccaaa aagaaaattt aaaaaaacta
      661 aagaaaactt ataaagaaca agcaattccc aaagggctta aagaggagat ccgccagcgt
      721 tttattatcg aagagcaatc atttataaaa gcaaagcgga gaaagagacg tctccaaggg
      781 ttcggagtat ctttggctat cgtaatgatt acaacaagta gccttctttt taatagtcaa
      841 gttcgaagct ttgcagaaga tttacccatc ttaggatcag tcattgaatt gatcttaggc
      901 gaacgcttta ctgatcgatc tgaaaaaatc gatattcagg taccgaagat caatacacaa
      961 aatgagaaag aaaataaaac aattcatgga ttaaatcaaa aatactttcg cgaaggacaa
     1021 gcagattttg aaaaagctga aaaagaatat ggaaattttg aaacggatca ttaccaagtt
     1081 ttgggagatt atcagaaaat cgtagacgat aaccggtttc tcgtgattga aaggcagatc
     1141 acccaaacag cagcagatag ccatgttgaa aaaagatacg acacaatcga caagaaaaac
     1201 agtgtccagc tctcacttcc acttttattc aaagatgaca cctatctatc tgttttgaca
     1261 aaagaagtca aacggcagat ggccgaacaa gtcaaggaag atccatctaa gtactattgg
     1321 actgagcaag atattcaaga agggacgata gaaaaaccaa cacttgttac gcctacacga
     1381 agtttttatt tgaacaaaga gcatcaatta gttttgactt tttctcaata cgaaattgct
     1441 cctggttata tgggtacacc agaatttgtc attcctaagt ccgtgacaaa aacgattttg
     1501 gcatcggaag attatttgga tcattagacg agaagtagtt gcgactagca gtttcaattt
     1561 tcaagtaagt acaagtaaat aataagaaga gaaaaagaga aagaagagat atagtgaaaa
     1621 gaaattattt agttatcagt attattgctt tatccgtgct tttgtttttc gaaattactt
     1681 cggtggcctc cttgatcacc gaagtaaaag gacaaacaca ctcttcggag cattcgtcac
     1741 aatctttcaa acaatcagaa caatcaacca ttaaaagcca atcaactgaa acagctaacg
     1801 tcaaagaagc cattcaagaa tcagcggaaa aggtacaaag tcagtcatca caatcaacac
     1861 aagacagtac aaaaacgagc cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg
     1921 atggaccagg agcgaccaca acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc
     1981 atgccacttt tttcgtctta ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga
     2041 cagctgaagc agggcacgag attggcaatc acacatacga tcatcagaat ctggccactc
     2101 tttctgccca gtcgatgacg gaggaagtga cgaaagcaga cactgagatc aaaaaagtca
     2161 ctggcaaaac ccccactttt gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa
     2221 cgatcatcca gcgaccgatc atcgaatggt ctgttgattc agaagattgg aagacaagaa
     2281 atcctgattt gattttgcag aagatccaag caacagtata cgatggggca attattttat
     2341 tccatgatat ctatcctgaa acgattcgtg ccgttcctca agtgatcgat tatctgaaag
     2401 agcaagggta tcgcatcacg acagttgggg atttattagg gcatccaacg gctgtcgaga
     2461 actattatgg cagaaacgat catcggccag ttcagtaagt gagacaagta tctttaataa
     2521 ataacacatt ttaataagta gttgaacaca taactacctt acttgcaaag gcttaacaat
     2581 atctatacag gtacaacaaa aaactgtcca ccaattttct ttggcggaca gttttcactt
     2641 aaaaataaag aagcaactat tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag
     2701 ggaatcgaac ccccgtctca agaaccggaa tcttacgtga tatccactac actaaggacg
     2761 caagcactgg gataattgta actaaacaga taaaaaatta caaggggatt ggacaaaaaa
     2821 atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga cacagaaact agccatgacg
     2881 caacagttgc aacagtccat ccaagtttta caatataaca gtgaggagct gttggcattc
     2941 gttgagaatc aagcaatgga aaatccattg gtggaagttg ttgagccgga atggcagcca
     3001 gattacataa aagcttcttc atcttcttat gaaggagaag agacaaatta cctaaatcag
     3061 attcctgata caaaagggtc tttatttgat tcattgatcg aacaagtcca tttgaattac
     3121 cgagatactt ttttgaggaa aatcgttctt tatcttgtag aatacatcga tttgaacgga
     3181 tttttgacga ttagtttaga agaagccgga aaagagattg gagctacgcc tatccaaatg
     3241 ctcgatgcgt tgactctgat tcagcaatta gaaccagctg gagtaggtgc acgtagtttg
     3301 caagaatgtc tcatgcttca aacagaacga gatgattatg cgccggaatt agcttatatc
     3361 gtgttggaag aatgttttga ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt
     3421 ttttctgttg atctacatgc tgttcagcag atattcgact atttacaaac gttgagtcca
     3481 agccctggcc ggattttcga ccgtagcagc gaattgtaca ttattcctga tgtccgggta
     3541 ttagtggacg acgataaaaa tgtacaagtt atctcaaacc gaaaaaatca gccaaatata
     3601 cgctttcaag agagttactt caaacaaatg tcacaacaag cagataaaga aactgaaaat
     3661 tatttaaaag aacggaaaca ggaattcgaa tggttgaaga aaacgatcct acaacgtggg
     3721 gatacaattt tgcgggttgc tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac
     3781 aaggaacgtc caatcaagcc tcttacgtta aaggaagttg caactgagat agatgtgcat
     3841 gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg aaacagactt cggtgtattt
     3901 gaattaaaaa aatttttcac tacacgaatt cctacgaata gtacagaaca gacagaagac
     3961 ctttcagcag atacagcgaa gaaaaagctg caggaattag tcgatcaaga agacaaaaac
     4021 aagccgttgt ctgaccagaa attggttgaa ttgctgaaaa aagatgagat agcgatctca
     4081 agacgaacag tagcaaaata tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa
     4141 cgttatgata actaatcagt caaaagacta gaaaaagtat ttcacttttt ctagtctttt
     4201 tagttgaaaa acgagagcaa aactgttaga cttacagtcg tgaggttgag atttcccttt
     4261 attgatgtga aattcgaata gttaatttct ttttttatcg tatatgggtc gttttaagtc
     4321 tacattggac gtaaagtgtc caactaagga gatcgctatg ctggatgaaa taaaaatgat
     4381 tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa
     4441 tatctattgg atgcagccga ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga
     4501 acgagtattg cgaacagaaa cagatctttt gaaaaatctg aatttgatcg acacctcaaa
     4561 aagcggtatg acgctgacaa ctaagggcga agaagtttat caaagccttg aaaacttcat
     4621 ggatcagctt ttaggtacgc atcaaaccga acaacagttg gcagaatatt ttggtaacca
     4681 acgctgtatc gttgtttcag gaaacagcga agagcagacc aaagtggctg atgcattcgg
     4741 cgaagcgctg agcgaagccc tagaccgact acttccagaa ggcgagaata tcatcgctgt
     4801 gatgggagga actactatgg ctgtggtagc cgaacaattg tctaatctgg aaaataagaa
     4861 acggcataac ctgtttgtcc ctgcaagagg cgggattggt gaagcgataa ctgtacaggc
     4921 aaattctgtc agtgcaagaa tggctgctaa agcaaacggt aaccaccgtg cactttatgt
     4981 tccggaacaa ttaagtctgg caacttacaa ttctttactc aatgagccat ctatccaaga
     5041 agtcttaaac ttgattagtg aagcgaattg tgttattcat agtattgggc gtgcattaca
     5101 catggcagca cgtcgtaaga tgactgaaaa agaattagtc atgctgaaac aagcaaatgc
     5161 tgttgcggaa tcttttggct actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc
     5221 tagaatcggc atagaattgc aagatttgga aaaagtcccg atcatcatgg caatcgcggg
     5281 cggaaaatca aaagctaaag caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg
     5341 gctcatcact gatgaagctg ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa
     5401 atattttttt gattttcata aggaggaaat cttaaatgac agttaaagta ggtattaatg
     5461 gttttggacg tattggacgt ctagcattcc gtcgtatcca agatgtagat ggaatcgaag
     5521 tagtagcaat caacgacttg acagatgcta aaatgttggc acacttgtta aaatacgaca
     5581 caactcaagg acgtttcaac ggaacagttg aagttcatga aggttcattc aacgtaaacg
     5641 gaaaagaagt taaagtatta gctaaccgca acccagaaga attaccatgg ggcgaactag
     5701 gcgtagatat cgttctagaa tgtactggtt tcttcacttc taaatcagca gctgaaaaac
     5761 atttaacagc tggtgctaaa cgcgttgtta tctctgctcc tggcggaaac gatgttccaa
     5821 caatcgttta taacacaaac cacgaaacat taactggtaa agaaacagtt atctcaggtg
     5881 cttcatgtac tactaactgt ttagctccta tggctaaaac attgaacgac aaatttggtg
     5941 ttgttgaagg attaatgaca actatccacg cttacacagg tgaccaaatg actctagacg
     6001 gacctcatcc taaaggtgac ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta
     6061 actcaacagg tgctgctaaa gctatcggtt tagtaattcc tgaattgaac ggtaaattag
     6121 acggagctgc tcaacgtgtt cctgtaccaa caggttcatt aacagaatta gtaacagttc
     6181 ttgaaaaaga agtaactgtt gacgaaatca atgcagcaat gaaagaagct tcaaacgaat
     6241 cttacggata caacacagac gaaatcgttt cttcagatat cgttggtatg acttacggtt
     6301 cattatttga tgctacacaa actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa
     6361 ctgttgcttg gtacgacaac gaaatgtcat acactgcaca attagtacgt actttagaat
     6421 acttcgctaa cttataagat agacaaacgt acaagtgtaa attatagaca tgtacattta
     6481 aaagcgggga agcaatcgcg cttcttccgc tttttttgat aataagttta ttcaggagga
     6541 aataaaatgg ctaagaaaac agtgaaagat atcgatttaa aagacaaaaa agttcttgtc
     6601 cgtgttgact tcaacgttcc tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa
     6661 gcagcattgc caacaatcaa ctacgttcta gaacaaggcg gaaaagcaat ccttttctct
     6721 caccttggac gtgtaaaaac agaagaagat aaagaaggaa aatctttagc tccagttgca
     6781 aaacgtctag gcgaattatt aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa
     6841 caattagaag aagcaatccg caacatgaat gacggcgacg ttgtcgtatt cgaaaataca
     6901 cgatttgaag atattgacgg taaaaaagaa agcaaaaatg atacagaact aggtaaatac
     6961 tgggcttcat taggcgatgt atttgttaac gatgctttcg gtacagctca ccgtgcacat
     7021 gcttctaacg taggtatcgc ttcaactggt atcccaacag ttgctggatt cttgatggaa
     7081 aaagaaatca agttcatcgg tgaagcagta gaagaaccaa aacgaccaat gattgcaatc
     7141 cttggtggcg caaaagtttc tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca
     7201 gataaaatct tgatcggtgg cggaatgact tacacattct acgaagcaaa aggaatcaag
     7261 atcggtaact ctctagtaga agctgataaa gtggaactag caaaagaatt aatcgaaaaa
     7321 gcaggcgaca aacttgtctt gccaatcgac aatgtctgtg caccagaatt ttcaaatgat
     7381 gttgaaacac aagtaatcga aggagacatt cctgatggtt tgatggcttt agacatcggc
     7441 ccagcatcag taaaattatt tgctgataca ttacaaggtg caaaaacagt tgtttggaac
     7501 ggacctatgg gtgtatttga aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa
     7561 gcaatcgcta acttagaagg cgcaacaaca atcatcggtg gcggagactc agctgcagca
     7621 gcagaacaat taggatttgc tgacaaattc acacacatct caacaggtgg tggcgcaagc
     7681 ttggaattgc ttgaaggtaa agaattacca ggactagcag caatcaacga taaataaacg
     7741 accaagtttg ctcaattctg acgagtaatc gatcaaaagg tgaagggagt tttcatgact
     7801 ccctagccat ttttcaataa attaaggagt gtgttcctca atgcgtaaac ctattatcgc
     7861 aggtaactgg aaaatgaaca aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa
     7921 aaacaaaatc ccttcaaatg acaaagttga ttcagtcatc ggatctcctg cattattctt
     7981 acaagaatta atgacatcag ctgaaggtac agaactaaaa atctcagcac aaaactgcta
     8041 ctgggaaaac tcaggtgcgt tcactggtga aacttcacca gcagctcttg ctgatctagg
     8101 tgttcattat gtgatcatcg gacattcaga acgtcgtgaa tatttccatg aaacagacga
     8161 agacatcaat aaaaaagcaa aagctatctt tgcaaatggt atgacaccaa tcctatgctg
     8221 cggcgaatct ttagaaacct atgaagcagg caaaactgct gaatggatcg aaggacaaat
     8281 tacagctggt ttagctgatc tttcagctga acaagtgtca aacatggtta ttgcttatga
     8341 accaatctgg gcaatcggta ctggtaaatc agctgacgca caaatcgctg acgaaatttg
     8401 tggagttgta cgtcaaacag ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg
     8461 tatccaatac ggcggttctg taaaaccaga aaatattgct gaatacatgg caaaagaaaa
     8521 tgttgacggt gctttagtcg gtggcgcaag cttgcaagca gattcatttt tgtcattgtt
     8581 agacgctgta aaataattgt tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg
     8641 agcaaaatta actaaaatcg tatatgaggg aaatccctta ggaaaaaaca aactcaaagg
     8701 agagacaaaa catgtcaatt attactgatg tttacgctcg cgagatctta gactcacgtg
     8761 gtaacccaac aatcgaagta gaagtataca cagaaagcgg agcttttggc cgcggaatgg
     8821 ttccttcagg tgcttcaact ggtgaatacg aagcagttga attacgtgac ggagacaaag
     8881 ctcgttatgg cggtaaaggt gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg
     8941 aagcaatcat tggctacgat gttcgtgacc aaatggctat cgacaaagct atgatcgctt
     9001 tagacggaac tcctaacaaa ggtaaattag gtgcgaacgc tattttaggt gtttctatcg
     9061 ctgttgcacg tgcagctgct gactacctag aagtaccttt ataccactac ctaggcggat
     9121 tcaacacaaa agtattgcca acaccaatga tgaacatcat caacggcgga tcacatgctg
     9181 ataactcaat cgacttccaa gaattcatga tcatgcctgt tggcgctcca acattcaaag
     9241 aagctctacg tatgggtgct gaagtattcc acgcattagc ttcaatcttg aaagctcgcg
     9301 gattagcaac ttctgttggt gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag
     9361 aaggttttga agtaatcatc gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg
     9421 ttgttcttgc tatggatgct gcttcttcag aattctacga caaagaaaaa ggtgtttacg
     9481 tactagctga ctcaggcgaa ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag
     9541 aattagtttc taaatatcca atcatctcaa tcgaagatgg attagacgaa aacgactggg
     9601 acggattcaa gaaattaact gacgtattag gcgacaaagt tcaattagtt ggtgacgact
     9661 tgttcgtaac aaacactcaa aaattatctg aaggtatcga aaaaggaatt gctaactcaa
     9721 tccttatcaa agtgaaccaa atcggtacat taacagaaac tttcgaagct atcgaaatgg
     9781 ctaaagaagc tggctacaca gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa
     9841 caatctctga tatcgctgtt gcaacaaatg ctggccaaat caaaactggt tctctatcac
     9901 gtactgaccg tattgctaaa tacaaccaat tactacgtat cgaagaccaa cttggtgaag
     9961 ttgcagaata caaaggtttg aaatctttct acaacttaaa aaacaaataa ttcttttcta
    10021 atgaagatac atgatcgact attgaaagat agtcgagtat gatcacttag ttaagtaatc
    10081 gaactctcta cttgctgaaa tatcagcagg tagagagttt t
//
LOCUS       DADXTG010000093        10111 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.094, whole genome shotgun sequence.
ACCESSION   DADXTG010000093 DADXTG010000000
VERSION     DADXTG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.094"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            105..254
                     /locus_tag="IWI57_001996"
     CDS             105..254
                     /locus_tag="IWI57_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBC2450130.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            265..1779
                     /gene="dltA"
                     /locus_tag="IWI57_001997"
     CDS             265..1779
                     /gene="dltA"
                     /locus_tag="IWI57_001997"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBC2450131.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1776..2981
                     /gene="dltB"
                     /locus_tag="IWI57_001998"
     CDS             1776..2981
                     /gene="dltB"
                     /locus_tag="IWI57_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBC2450132.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3026..3259
                     /gene="dltC"
                     /locus_tag="IWI57_001999"
     CDS             3026..3259
                     /gene="dltC"
                     /locus_tag="IWI57_001999"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBC2450133.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3262..4530
                     /gene="dltD"
                     /locus_tag="IWI57_002000"
     CDS             3262..4530
                     /gene="dltD"
                     /locus_tag="IWI57_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBC2450134.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4671..5753
                     /locus_tag="IWI57_002001"
     CDS             4671..5753
                     /locus_tag="IWI57_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBC2450135.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5753..6556
                     /locus_tag="IWI57_002002"
     CDS             5753..6556
                     /locus_tag="IWI57_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBC2450136.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6626..7366
                     /locus_tag="IWI57_002003"
     CDS             6626..7366
                     /locus_tag="IWI57_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBC2450137.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7377..8381
                     /locus_tag="IWI57_002004"
     CDS             7377..8381
                     /locus_tag="IWI57_002004"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBC2450138.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8426..>10111)
                     /locus_tag="IWI57_002005"
     CDS             complement(8426..>10111)
                     /locus_tag="IWI57_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450139.1"
                     /translation="KDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLD
                     EANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSS
                     PYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKR
                     LEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRM
                     FYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEA
                     YKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLID
                     HILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQ
                     TKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELP
                     INDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLF
                     SRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3232 a         1683 c         2059 g         3137 t
ORIGIN      
        1 tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca
       61 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
      121 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
      181 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
      241 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
      301 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
      361 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
      421 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
      481 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
      541 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
      601 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
      661 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
      721 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
      781 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
      841 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
      901 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
      961 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
     1021 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
     1081 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
     1141 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
     1201 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
     1261 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
     1321 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
     1381 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
     1441 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
     1501 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
     1561 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
     1621 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
     1681 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
     1741 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
     1801 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
     1861 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
     1921 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
     1981 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
     2041 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
     2101 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
     2161 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
     2221 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
     2281 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
     2341 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
     2401 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
     2461 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
     2521 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
     2581 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
     2641 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
     2701 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
     2761 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
     2821 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
     2881 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
     2941 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
     3001 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
     3061 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
     3121 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
     3181 ccctgtatct gaattcgagc gagaggattg gagcacaccg aataaaatca ttcaacaagc
     3241 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
     3301 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
     3361 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
     3421 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
     3481 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
     3541 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
     3601 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
     3661 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
     3721 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
     3781 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
     3841 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
     3901 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
     3961 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
     4021 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
     4081 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
     4141 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
     4201 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
     4261 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
     4321 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
     4381 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
     4441 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
     4501 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
     4561 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
     4621 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
     4681 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
     4741 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
     4801 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
     4861 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
     4921 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
     4981 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
     5041 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
     5101 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
     5161 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
     5221 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
     5281 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
     5341 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
     5401 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
     5461 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
     5521 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
     5581 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
     5641 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
     5701 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
     5761 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
     5821 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
     5881 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
     5941 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
     6001 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
     6061 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
     6121 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
     6181 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
     6241 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
     6301 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
     6361 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
     6421 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
     6481 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
     6541 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
     6601 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
     6661 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
     6721 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
     6781 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
     6841 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
     6901 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
     6961 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
     7021 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
     7081 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
     7141 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
     7201 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
     7261 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
     7321 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
     7381 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
     7441 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
     7501 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
     7561 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
     7621 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
     7681 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
     7741 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
     7801 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
     7861 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
     7921 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
     7981 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
     8041 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
     8101 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
     8161 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
     8221 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
     8281 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
     8341 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
     8401 ccacatatat ggattccgct attttttata caggattcgg gtaatttcga gagagttgaa
     8461 cagtgatccg atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg taacgatccg
     8521 ctacgtcttt catcatcatt aagtcaaaac gttcctgatc atctaattct tctgtgatgc
     8581 ctcgactaaa taggtttgta tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt
     8641 cctcttgttc gtgtatcgac aaataaattt ttcgatacag tggattttcg atggcatgag
     8701 tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg agccagatcg tcatcgttga
     8761 taggaagttc ttcgggaaga tctgcttgga aggttatctg aatattccgt ttggcagcta
     8821 acttctggta acgcgctaag ataagatttg tcacctggtg gttgcaaaaa gaacacagca
     8881 tttcgaaatt ttttgcttct tctaatatct tttccacata gtttttagct tttgtttggt
     8941 ccgtatgcat taaatcttgc agattctcca tctcttggac aaattccagg cgaagtgtat
     9001 ttacttcttg gatatgctgt cgaagctgct cataatgttg ctccaacaaa tcatttttga
     9061 tttttaagaa atctacttgt ctccgatctt tgtcggtatt taagatgtga tcgattagat
     9121 tgtagctgat aataacgatt agaatgataa tatataaaat cgtagaccaa ttgatcgttg
     9181 tttgatagat acctaatgcc gtttggatgt aaatcaagca atggataatg gctaccaaga
     9241 cgatccaagg tgtacaagct acataaaaac ggttattctt atatgcttcg ccaatgcatg
     9301 caagtgaagt gactaaagtg cttataacac tagcaatatt agcaatcggc attaattcag
     9361 gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc aaaagttgga
     9421 acgtacaaaa aattccgtac cattttttat agtaaaacat tcggctccaa tagtagcaga
     9481 tgatgaaagc tgacgttaag ataataaata aatgagataa aaaagaatga acgaatggtt
     9541 caaaaagaat accacttaaa atatcttctg atacagcttc aaatcctaat gccaaagcaa
     9601 agcaacttaa gagaataagt gagtattcaa gacgtttttg tttatacatc aagtagatcg
     9661 tatagaaaaa ggtccctata gcgataaaaa cagcgattat catcgtcaat acttgcggca
     9721 ctgattgtga ataaataaaa ctaatcaatg gtccagttgt tcctagataa actttcggtg
     9781 gcagtccagc atagttctga taaggagatg agactgtaag ggtcaatttt tttccgatat
     9841 aatttgctgg taaatctatc acagccaatg agagtccggg attgctttct tcctgataat
     9901 ctttccgttg gtataaaatc ttttcgccta gatatacagt aatccattga tggtttcctt
     9961 tgattagtaa atttgcttcg tccaacggtt tttcaagtgt cgtttccata atgaatgttt
    10021 cgtctttatc cacggtagga atcgtacgag tgtacttgct gtcaaactta aaatcaggat
    10081 catcttttgt atagaaaacc cacgaatctt t
//
LOCUS       DADXTG010000094        10035 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.095, whole genome shotgun sequence.
ACCESSION   DADXTG010000094 DADXTG010000000
VERSION     DADXTG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.095"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(258..2378)
                     /locus_tag="IWI57_002006"
     CDS             complement(258..2378)
                     /locus_tag="IWI57_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBC2450140.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2608..3786
                     /locus_tag="IWI57_002007"
     CDS             2608..3786
                     /locus_tag="IWI57_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBC2450141.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3802..4560
                     /gene="aroD"
                     /locus_tag="IWI57_002008"
     CDS             3802..4560
                     /gene="aroD"
                     /locus_tag="IWI57_002008"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBC2450142.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4583..5068
                     /gene="ybaK"
                     /locus_tag="IWI57_002009"
     CDS             4583..5068
                     /gene="ybaK"
                     /locus_tag="IWI57_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBC2450143.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5139..6392)
                     /locus_tag="IWI57_002010"
     CDS             complement(5139..6392)
                     /locus_tag="IWI57_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2450144.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVYLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6509..7858
                     /gene="gdhA"
                     /locus_tag="IWI57_002011"
     CDS             6509..7858
                     /gene="gdhA"
                     /locus_tag="IWI57_002011"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBC2450145.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7970..9316
                     /locus_tag="IWI57_002012"
     CDS             7970..9316
                     /locus_tag="IWI57_002012"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBC2450146.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9624..10010)
                     /locus_tag="IWI57_002013"
     CDS             complement(9624..10010)
                     /locus_tag="IWI57_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBC2450147.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3216 a         1814 c         2049 g         2956 t
ORIGIN      
        1 attaatcgag cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata
       61 gaaaatgaat atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa
      121 aatattttgt aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc
      181 aagcttttga acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc
      241 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      301 gtttgcgttt tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt
      361 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      421 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      481 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      541 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      601 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      661 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      721 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      781 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      841 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      901 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      961 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
     1021 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
     1081 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
     1141 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1201 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1261 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1321 ctaatgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1381 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1441 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1501 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca
     1561 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1621 ttttttcctc tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca
     1681 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1741 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1801 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1861 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1921 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1981 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     2041 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     2101 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     2161 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2221 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2281 aaataggcaa aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc
     2341 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2401 cttaatcaca gaagtgtcct gaaaccatgt tacacagctc acttattctc atttatttta
     2461 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2521 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2581 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2641 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2701 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2761 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2821 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2881 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2941 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     3001 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcatagg aggctatgag
     3061 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     3121 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3181 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3241 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3301 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3361 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3421 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3481 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3541 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3601 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3661 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3721 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3781 cattagaagg agcgatgcag aatggttcgg gtaaagggat tgaatatagg aagcggtaga
     3841 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3901 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3961 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     4021 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     4081 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     4141 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4201 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4261 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4321 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4381 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggttggc
     4441 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4501 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4561 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4621 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4681 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcgta tatttaaaac
     4741 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4801 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4861 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4921 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4981 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     5041 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     5101 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     5161 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5221 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5281 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5341 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5401 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5461 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5521 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5581 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5641 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5701 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5761 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5821 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5881 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5941 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     6001 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     6061 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaata tacgccttgt
     6121 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6181 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6241 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6301 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6361 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6421 tctgaagaaa gaaaacagaa aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6481 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6541 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6601 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6661 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6721 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6781 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6841 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6901 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6961 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     7021 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     7081 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     7141 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7201 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7261 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7321 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7381 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7441 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7501 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7561 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7621 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgtccagcg
     7681 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7741 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7801 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7861 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7921 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7981 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     8041 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     8101 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     8161 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8221 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8281 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8341 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8401 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8461 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8521 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8581 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8641 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8701 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8761 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8821 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8881 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8941 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     9001 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     9061 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     9121 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9181 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9241 caagaaaaac atgttcgcac ttctaggcaa accaggcttt gaagatcttg ctaaagaatt
     9301 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9361 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9421 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9481 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9541 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9601 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9661 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9721 aagatcgttc cttcggggta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9781 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9841 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9901 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9961 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
    10021 cttttttatt tttat
//
LOCUS       DADXTG010000095         9681 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.096, whole genome shotgun sequence.
ACCESSION   DADXTG010000095 DADXTG010000000
VERSION     DADXTG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.096"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            117..311
                     /locus_tag="IWI57_002014"
     CDS             117..311
                     /locus_tag="IWI57_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBC2450148.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            314..1516
                     /locus_tag="IWI57_002015"
     CDS             314..1516
                     /locus_tag="IWI57_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2450149.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2099..3730)
                     /locus_tag="IWI57_002016"
     CDS             complement(2099..3730)
                     /locus_tag="IWI57_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBC2450150.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3744..5417)
                     /locus_tag="IWI57_002017"
                     /pseudo
     CDS             complement(3744..5417)
                     /locus_tag="IWI57_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
     gene            complement(5430..5747)
                     /locus_tag="IWI57_002018"
     CDS             complement(5430..5747)
                     /locus_tag="IWI57_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBC2450151.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5772..7172)
                     /gene="lacG"
                     /locus_tag="IWI57_002019"
     CDS             complement(5772..7172)
                     /gene="lacG"
                     /locus_tag="IWI57_002019"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBC2450152.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7409..8194
                     /locus_tag="IWI57_002020"
     CDS             7409..8194
                     /locus_tag="IWI57_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2450153.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8197..8889
                     /locus_tag="IWI57_002021"
     CDS             8197..8889
                     /locus_tag="IWI57_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBC2450154.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9163..9534)
                     /locus_tag="IWI57_002022"
     CDS             complement(9163..9534)
                     /locus_tag="IWI57_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBC2450155.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3057 a         2055 c         1701 g         2868 t
ORIGIN      
        1 gttgggaagt cctgaacaag tgatggattt tcaagaaagt accgttcatt atacaaaaat
       61 aaagaaataa cactattacc caatgatagt gttttctttg aaagtgagtt tatacaatga
      121 aaaatacaat ttgtcgaaaa gatttagagg ctctaggatt taaaacacat caagctacca
      181 caataatccg acaagcaaaa acaagaatgg taaatcaggg atatccatat tataataata
      241 aaagagttgg ggtagtaccc cgacaagtaa ttgaagaaat tataggtgtt cctctttcac
      301 aggaggaata actatgaaaa caaaataccc ttgtgtgtat caagataaaa atggtcgctt
      361 ttcttatcta gttgagttag gaacagatca agtgactgga aagaggatcc agaagaaagg
      421 aacaaaagat tctctaggaa aacgattctc atcagctcgt gaagcacaca aagaagtcat
      481 gcgtataaaa aatgaataca tggttgtaaa tggatttgcg aattatgatt taacttacaa
      541 agaatttatg gagcagtctt acgttccaca ctacaaagct agcgtacaaa aaagtacttg
      601 gagttctcgt gagtgtggat tgattcagat tactgaattt ttcggtgata agaagcttcg
      661 tgaaatcaat gtgcgagatt gtgaaaatta tcgaatttgg ttattgaacg atagcggata
      721 ctcacaagca tacagttcac ttatgtacgg tatgtttcgt aaatcattag actatgcggt
      781 caccctgcaa tttctgaatg agaatatttc caaaagaaca aaagcaattc caaaaggaaa
      841 atcaatcgtt ccttactgga caaaagaaga atttgaagaa gttttggcaa aaatttataa
      901 agatgatttc tacgaacata tgtgtttttt aatgatctgg ctatattaca tgactggtgt
      961 tcgtgtaagc gaaggattag cacttaattg gaatgacgtg gacttcaaac gtaaaaagtt
     1021 gagggttcat cacaccttgg aaatgacaaa tcaaaaagat tttattcgga aaccttacac
     1081 aaaaactgaa agcggcttgc ggatcatttc tctggacgat gatacaattg aagtgctaaa
     1141 aaagtggaaa gttattcaaa aaaatcatgg tgtagaacaa tttattctta gctacactga
     1201 cctcccactc tatcgttcaa ctgttcaacg aattattcag cgttatgcca aactagctaa
     1261 ggtaccccta atacaaggaa aaggattacg tcattctcat gttagctacc taatcaatga
     1321 gtttaacgca gacattctgg tcgtgtcgca acggttaggc cattctagcc ctgaaatcac
     1381 tttaaagcac tacgctcatc tatggagccg taatgatgaa ggaattgcag aacaaatgac
     1441 aggaaacatt aaatttacct gtgcaacaaa atctaaagtt gcaaacttta atggaaatca
     1501 agcagtaaaa atctaaccct accatttccc taccaaaatg aggaataggc tgaaaaagca
     1561 ctgatataat agcttcttta cgaggctact catattgagt gggaataaag agccaacata
     1621 atatggaggg ggaatcccta taaatcaaca ttcttcaata gctaaaatgc ttgattttca
     1681 ttaaaaataa gatggttccc taccaaaaga gactataata taagttaatt tgtttcattc
     1741 ttatcataaa aagaagttct cgaaaaacga actgttttcc gagaactttt ttctatccat
     1801 ctaagtgctc aaatcatatt cacgacggaa ttctaaggtt accctttctt aaacaaactt
     1861 tatgttatct gcacaacttt ttgcaatttt ttcattgctt cttacgaaat cgttgaactc
     1921 tctttagcat aatacaaagt gtagatgaag tctggtgctt tactcgtttt ggtaaaaatg
     1981 actggaaatc gtttctggtt atttttggca aaggcgaggt gcaggttcga ttcctgcctt
     2041 ctacgctaac gatgtggctg aagggaacct ccgatcttga atcgaaacga tccgcagtct
     2101 attgtttcac caatgaaaca aaatcaacgt tcgtccaagc atttcctgaa gccttgctat
     2161 aaaccccaat ttcacaaaca cctgttgtaa ttggaatgtc tttgattgta atttttgtcc
     2221 attcacttgt tttaacaacc gcagtattta gttccgctcc accataattt ttgatatata
     2281 actgattagt atcaaactct cccccagaca ttacataggc actcaatgtg taagttcctt
     2341 tgcttatgcc actaacattt tgataagctg atgcctcgta tgcttgatcc tgccaaaaag
     2401 ttagtttgta gtccccaaaa aaagtgcccg cttccgtttt gatcgtaccc ttagcggttc
     2461 cttgttgaaa ccaggtttgc caattggaag gactggtcgt tggtccatcg ctttcgaaac
     2521 ttgcattttt aactagattt atttgcgccc ctgcttgttt aaacgcacgc aagcttggca
     2581 aggcgtttcc agcaaaatca aacatcgctt ggttgtccca ttcgtttccc atagtggatt
     2641 gaacactgag ataattcata cctgcttgtg ttgcccaact cacatttccg ttgtaccaaa
     2701 gaggttccca atagaagaag cccgccccgc gattattcgg tactgcctga atccgatcgg
     2761 caagatccag caaatactct gcttgtcctg tcggtgttgg ttcatatccc cccgcagctg
     2821 cttcggcttc accaaaagca tttggcatag tatcggcatt cgctgttgta tgcccataag
     2881 ctgtttcaac tacgatcact tctttaccat accgttggct gatcgcattc atattggttt
     2941 gaagttcatt cattgaaccg tgccaatacg gataatacga cactccaatt aaatcaaatg
     3001 acacacctcg actcgtgatt tcatcaaacc accaagtaaa tgctcctgta tcgccaccat
     3061 cagctaagtg gagcattact ggagttgttt taggttgagt gtctcgaaca gcttggatac
     3121 cagacttcaa aaaagcagcc aatcgatcaa attctccccc gccttctccc caagacttgc
     3181 cataaggcca gagcattccg ctattcagtt cattgccgat ctgaaccata tcaggataaa
     3241 cgtccagctg cttcatttcc gacagaacgt tctttgtata gtcgtacaat gccgtattca
     3301 tttcttcaaa ggtcagacct tgccatgctt taggaagatt ctgtttccca ggatcaaccc
     3361 aaaaatcact gtagtgaaaa tccagcagca ccttaagtcc cagatttttt gctcgttttg
     3421 caaggtcaat cgtcgtttcc agatcatttg tccctgctcc ataactttct ccattactat
     3481 tataaggatc ctgccacaaa cgcagtcgaa catagttgac accattattt tttaaaatct
     3541 ctaaggcatc ttttgaaata tcgtcttcat aatattttgc accagatttt tccatatctg
     3601 acaggatcga aatatctgct ccgcgaataa attcgctggc agatgcttca acagtttttg
     3661 aaacagcccc caaagcccct aacacagctg caaataaaac agctttacgc aaccaaattc
     3721 ccattttcat tatggctcct cctttattga aattggttca gaataaactg aacagctttt
     3781 tcaggatctc tcgttaatcc gatatattct gctccttttg tcgcaaccag cttgattcct
     3841 aagcgatcgg tgtcttcttt gatatcatca tagtaagaat tgacttgcgg tgctaagacc
     3901 accatatcaa aatcctgcag catttcgtaa tgagaaccat aagcacccgc cattgcactg
     3961 atcggtgctt tcgattcaga tgcacctttt ttttaagcat tagctaacat cgcacttgtc
     4021 cctgcgccag cacaaagtac caacacattg atttctttat ttgcatcgct gataacttga
     4081 ctcgctgcca caggtacttc ctcacgtaca tcggcagaag caccttcctc ggttgcgatc
     4141 acctctgctt tttgtgcttc tttctctact aagacagcat cgtatgcttt gcaaaatggt
     4201 agatagatca ggaagtcaac gacgattaga accgctgcta gaacaaaagc taagataccc
     4261 atgcccgttc ctagaaccaa accgatcggt gctggagttg cccaaggcaa aacgtacata
     4321 aagctgttca tgcccaatac atcgacaaaa aacttgaaca ggcaaacgtt tacaacaggt
     4381 gttaacaaaa atggaataaa aaaataaggg ttcaaaatca tgggtgccgc aaatagcaat
     4441 ggttcgttga cagcgaaact tactggaata aaggaagctt tcccaactgc cttcaactgc
     4501 ttagacttag caaagagtag gaagataaaa ggaacaacca atgttgcccc agttcctccc
     4561 atagttccta caaaattccc taaacccacc gtcagtactt tagaagcttg ttcaccggaa
     4621 ttgaacaatt gcagattggt ttccacattg gcatagataa ttgccgcaat tgccggctcc
     4681 acgatcgacg gtccatgaac accgacaaac caaaacagcg ccatcgctcc ccagataata
     4741 gcgattccta agtacccgtc agctgctgta aatagcggct gcagcaccgt aatgattgct
     4801 tctgcaaatg agatttggaa gatttgtcga atgcctaaat caattaaaac ggccgcaaat
     4861 actgaaaaag agaaaggaaa aatatctcgg aaggtttgcg aaatcgttcc aggaacttct
     4921 ttaggcattt taatggtaat atctttcagc acacaaaact tatataaatt aactgttaaa
     4981 aatgcagcga caaaggaagc cagtagtccc tttgtcccca tatattccgt tgaaaatcca
     5041 ccttcaatcg gagagacgct taacaacagg aaacttacca cagaggccag catcacagag
     5101 gtggtgttga ttactttgtt tttcggtaag cgacgattca tcgaatcagc caagcagcga
     5161 gcagtggttg ctgaaacgag taaccctaca acacccatcg aataattata gactttccat
     5221 agccatgtgg agacagattc aggccattgg taaccaaaaa tctcaggaac cgcagcaatc
     5281 aaaatgaaaa tgcttgaaaa taaaatagct ggcatcgctg ttaaaaatcc gtcacgaacg
     5341 gctcctaaat aactattacg tgagattttt tcaaaaaatg gtttcccttt ttcgatttgt
     5401 ttaatgattc catccatagc gttcttctcc tatccttgat tctgtctgta cagtacaata
     5461 aagtcttgaa tcagatctct cagcaataaa gtggtcatta aatgatcctg cccatggata
     5521 aaaatgaatc ccatctccat ctctttccca gatgcttctt ccgctaaaat ttttgtttgc
     5581 gaattatgtg caagggttaa attttcgtcg gcttctttca acgccgaatc aacatgttca
     5641 aagtttcctt gtcttacttc tttcaataat ttcaataatg tactgcgagc gtcgcctgaa
     5701 tatgcaacga tctcaaaacc aagcatttgt aattcctctt tattcatctt ttcttcctcc
     5761 attatcactt attaaataat cgttttggtt tcactgacca acttgtacca gtaagctgat
     5821 tcttttgggt aacgcttctg ggtttcaaaa tcaacataaa acaagccata ccttttgtta
     5881 tacccatttg tccatgagaa aagatccata agcgaccaca aaaaataccc cttcacattt
     5941 accccggctg ttatcgcctt gcttaaggat tccaaataga ctctcaaata gtcgattctc
     6001 ggctgatcca taataatgcc atcttcaaac tgatctttat accccatccc gttctcagtg
     6061 atatagattt tgttgtaatg tgggtaatcg cttttaattc gcagcaacaa gtcatataga
     6121 ccttctggat aaatcagcca gtcccaatca gttcgaggaa ttccttcttt ataaatacgt
     6181 tctccgattc ccttcacttt ataaacagag gtcccttttt cacccgtacc gttatgatgg
     6241 attgcatttt ctccatcgta agctttgaca aaatggcatt ggtagtgatt gatccctaag
     6301 tagtcattac gcgtggatgc ttttttcaat tcaacgaaat cttcttcagg aaaatgatag
     6361 ctggcctgat ttgcttcaca tatctcatca agagcagtca aggtttcagt tgaataatag
     6421 cctaaatagg tagcatctaa taagaaacgg atcgacaatg catcgtctaa aaaggcagca
     6481 tgcttatctt ccggcgcgtc tgttgccgca tatttcgtct ctaatgagtg aactacacca
     6541 atttcacccg gaagttcgtt ttctttgaaa tagttcacta cacgggcatg agcaaccatc
     6601 atattatgaa gacaggcgac gatctttgta aaatcatatt tgattcctgg tgggaaaaca
     6661 cctaacaaat attgattggt tgcaactgga tagatttcat taaaggtact ccacaccttg
     6721 acttctttga attcatgaaa acaaaaaatg gcataggaaa caaatgcttc tattgtttct
     6781 cgattcaaaa aatctccatg gtcaaacaaa cgtttagggg tatcaaaatg atgcaacgtc
     6841 acaaacggcg ttacgtttct tttcttacat tcttcgaaca cacgatgata aaaagcgatt
     6901 ccttctggat ttggtttccc agctccatca ggaaaaatgc ggctccaagc aatggataat
     6961 cttagaccat ttacaccaaa acgttcacac aattcaatgt cctttgcata ttgatgataa
     7021 aaatcacttg ccggatcggg actgaatcgc ccttgttcct ctaaaaagtc atcccaagca
     7081 acagcgcctt tgccaccttc cttagtggct ccttcaacct gataggctgc tgtggctccg
     7141 ccaaaaataa aatcttctgg taatttaagc atgtacatct ctcctcaaat ctataatcag
     7201 ttttcgctct ttctaactga ttgcattgta aacgcatcca aaacatttgt caacaaaaac
     7261 aaacacaaat aatcataaaa accagaaaac agcaataaaa tctattgtaa caaacataaa
     7321 aaccacccta aatgtttact tttgttcgaa ttagattgac tgttgtccgt ttatatgata
     7381 gattatcctt aaattagagg tgaagacaat gttgaaaaaa gaaagattgc ttactatcgt
     7441 agaaatggtc aataaaaaag gaatccttac cgttaatgaa atcatcaatc aattagatgt
     7501 ttctgatatg accgttcgca gagatttaga tgaattagaa aaatcgggca agctgctgcg
     7561 tgtccatgga ggggctcaaa gtctttccta cactttagat caagaacttt ctcacactga
     7621 aaaagccact ctgcagattc aagaaaaaaa agaaattgct gccacagcag cctccttgat
     7681 tcacgaggga gaaacgattt tcttaggacc aggtacaaca atcgaactat tggcgacgca
     7741 tttactgaac aagaaaattc ggatcattac aaataattat ccagtttttg atattcttgc
     7801 tcaagcagat ggaccagaaa ttctactgat tggcggtgat ttccggaaaa ataccggcgc
     7861 atttgtcgga ccgatcacca acgacaattt aagaagacta aagtttacaa aagcatttat
     7921 cagttcaaat ggcgttcaca acgaagcgat ctctacttac agtaccgaag aaggagaagc
     7981 acagcagatc gctctaaata attcccgtaa gaaatatcta ctcgttgata ataaaaaatt
     8041 caatcgagat gatttttatg tgttttataa tttgcatgat ttcgatctgc tcattacgga
     8101 tcgccagatc tcgcgggagg ttcaagagca ctatgcacaa tatgtcgatc tgcaaattgc
     8161 tgattcaacc gaaaatacca ctataggagc gtaactatga aagggattat atttgatttc
     8221 aacggaacaa tgtttttgga ctctcatctg catgaagctg cttggctgca tatgatacat
     8281 gatcatacaa aagatggttt atcagacgag gatattttaa aaaatattca tggtcggacg
     8341 aatacggaga ttttgaaaca tttcatttca gagcatttga ccgcctctga gatcgccgta
     8401 ttgtcagaag aaaaagaaag ctattatcgt atgctttgct tgcaaaatga aaacgagctt
     8461 cagttgacaa aagggcttga agcgactctt gaccagctga ctgaccaaca gattccatta
     8521 acgattgcca cggctactgt caaagagaat gttgcttttt attttgatat tttcgattta
     8581 gcccgctggt ttgactggga caagattgtc tatgacgacg gctctttccc tgggaaaccg
     8641 caaccggata tttttcttaa agcggcagac aaactgagct tagctccggc tgattgtctt
     8701 gttatcgaag atgcttactc aggattactt gctgccaaac gtgcggggat cggaacaatt
     8761 attgcaattg atccattcgg taaaaacaaa acagtatttg aacaggcaca gctaaataaa
     8821 gatggaacca tcaaagattt tactggtttt tggacagagc acctgtctgc cacatccgtt
     8881 aattcttaaa cgattcaact aactaccaaa gaaaagcaac tgaaatgcac acctcctttt
     8941 ataaaaaact gccagtgaaa cgacatgaaa gatacactgc tatatcctta gtgaaatata
     9001 gcagtgtatc tttgactttt tcattctttt aaccatatga cgcgttcatc tctatctata
     9061 attaacatta aaaatagtgt tttcacatta tttctcaaac caatcttaaa aatcatcagg
     9121 agtacttttt tgatatactt ttattaatgc tgggcgtaat ggttaaaaga tttccactag
     9181 ttcctctcca ccaagctgag ttgaaaaatg cttatctgta aacaacatag acaaagagga
     9241 aataaacgtt tccgtatctt tctctgtccc aaggatatta attaattttc ctactaggta
     9301 agtatcatta aacttaagat ctgccttcac atcaaaagtt ttgataaaag cttttatcat
     9361 gcgtacaatt ttgtctttat cgccataatt gatttcacca ctcatatcaa tatagtcact
     9421 aatccctgaa gaaataatgg caactgcttc ttctttcgcc ttatcagata attcatttaa
     9481 tgactgggat tgattgatga cttcttgagt tttatctgtt acgtaatctc tcattatcct
     9541 atcggcatac tccgcaaagt aatcttgaaa aaattcttct acagggtaac caacttcgtt
     9601 ttcccaacca ggagaaattt cattatcagt acttattaaa tctattaaaa accaacccgt
     9661 tctctgagtg ttgattctat a
//
LOCUS       DADXTG010000096         9525 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.097, whole genome shotgun sequence.
ACCESSION   DADXTG010000096 DADXTG010000000
VERSION     DADXTG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.097"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IWI57_002023"
     CDS             185..1183
                     /locus_tag="IWI57_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBC2450156.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IWI57_002024"
     CDS             1295..1804
                     /locus_tag="IWI57_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBC2450157.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IWI57_002025"
     CDS             complement(1834..2493)
                     /locus_tag="IWI57_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBC2450158.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IWI57_002026"
     CDS             complement(2495..3259)
                     /locus_tag="IWI57_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBC2450159.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IWI57_002027"
     CDS             complement(3267..3944)
                     /locus_tag="IWI57_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBC2450160.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IWI57_002028"
     CDS             complement(3981..5366)
                     /locus_tag="IWI57_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBC2450161.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IWI57_002029"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWI57_002029"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWI57_002030"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWI57_002030"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBC2450162.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IWI57_002031"
     CDS             6305..7162
                     /locus_tag="IWI57_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450163.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IWI57_002032"
     CDS             complement(7243..7578)
                     /locus_tag="IWI57_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450164.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IWI57_002033"
     CDS             complement(7645..9015)
                     /locus_tag="IWI57_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBC2450165.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..>9525)
                     /locus_tag="IWI57_002034"
     CDS             complement(9093..>9525)
                     /locus_tag="IWI57_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2450166.1"
                     /translation="EFCGCSTYPIYTHFKSISGLKEKILEEITVYFENYLAECSSDDV
                     LSIVYLLKDFFSIHESIREIIAKSDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLN
                     ALGSLRSDDSSQDFLEMMNAVWDKNEVTDEYLENILKEQSP"
BASE COUNT         2794 a         1958 c         1610 g         3163 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actct
//
LOCUS       DADXTG010000097         9367 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.098, whole genome shotgun sequence.
ACCESSION   DADXTG010000097 DADXTG010000000
VERSION     DADXTG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.098"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            42..923
                     /locus_tag="IWI57_002035"
     CDS             42..923
                     /locus_tag="IWI57_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450167.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            936..2546
                     /locus_tag="IWI57_002036"
     CDS             936..2546
                     /locus_tag="IWI57_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBC2450168.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2623..3282)
                     /locus_tag="IWI57_002037"
     CDS             complement(2623..3282)
                     /locus_tag="IWI57_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBC2450169.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3565..4230)
                     /locus_tag="IWI57_002038"
     CDS             complement(3565..4230)
                     /locus_tag="IWI57_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBC2450170.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4377..4982)
                     /gene="dhaL"
                     /locus_tag="IWI57_002039"
     CDS             complement(4377..4982)
                     /gene="dhaL"
                     /locus_tag="IWI57_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBC2450171.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5010..6020)
                     /gene="dhaK"
                     /locus_tag="IWI57_002040"
     CDS             complement(5010..6020)
                     /gene="dhaK"
                     /locus_tag="IWI57_002040"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBC2450172.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5995..6441)
                     /gene="dhaM"
                     /locus_tag="IWI57_002041"
     CDS             complement(5995..6441)
                     /gene="dhaM"
                     /locus_tag="IWI57_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBC2450173.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6544..6864
                     /locus_tag="IWI57_002042"
     CDS             6544..6864
                     /locus_tag="IWI57_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBC2450174.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6976..7845)
                     /locus_tag="IWI57_002043"
     CDS             complement(6976..7845)
                     /locus_tag="IWI57_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2450175.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7941..9245)
                     /locus_tag="IWI57_002044"
     CDS             complement(7941..9245)
                     /locus_tag="IWI57_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2450176.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         2497 a         1969 c         1776 g         3125 t
ORIGIN      
        1 cttgtttagt atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag
       61 tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg
      121 tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga
      181 ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag
      241 atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct
      301 ctttgtgggg aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa
      361 aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa
      421 aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg
      481 ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa
      541 cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt
      601 ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa
      661 cactcgatga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta
      721 tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc
      781 aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg
      841 aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat
      901 tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt
      961 ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg
     1021 gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag
     1081 gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa
     1141 ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc
     1201 ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa
     1261 aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa
     1321 actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca
     1381 gctgtacgat gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg
     1441 ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac
     1501 tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc
     1561 ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct
     1621 ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt
     1681 tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca
     1741 ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc
     1801 gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt
     1861 tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg
     1921 acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca
     1981 cgatcatggt agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt
     2041 tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga
     2101 accaacttta ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga
     2161 ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg
     2221 atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg
     2281 gaattagtgg ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact
     2341 tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt
     2401 ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg
     2461 tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata
     2521 ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca
     2581 aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg
     2641 ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa
     2701 taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt
     2761 cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata
     2821 gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt
     2881 tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa
     2941 gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc
     3001 aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc
     3061 tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc
     3121 ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc
     3181 cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt
     3241 gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa
     3301 acagctattt ctaataaaga aacataactt cccttattcc aactgataat tttcttttat
     3361 catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc
     3421 ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata
     3481 tttacttttt gaataatcaa aaacaaacct tcactcgcaa gccgagagta aggtttgttt
     3541 ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc gcacctagct tttgatatag
     3601 tggtgctagt tcttgtaagg tttcagcggt tatttgctca acgtgttcga tttcttgcgg
     3661 atagaagaat agtttaccca tttgaaaggt tcggtctctc atcgcttttt tcaactcttc
     3721 ttgtttcttt gtatcggaca aatagtcaga caaagctaag tggtcgtcaa gttcatgttc
     3781 tggttgcggc catatgtaat aatccttgat atcgactgtt ttgctccact ctttcagtat
     3841 cgcaagcaac tggttatacg ctgcaatgct accatatcgt tcttcgcttt tataatgttg
     3901 gtacatcaaa tagatttggt attgtttttg attcaacaaa actgctagac aggcattctc
     3961 gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt
     4021 cgtccagctt tcgattttag gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt
     4081 ctggagctga atctctttcc atttttgcca gtgttcttta tattctttct tgatttgttc
     4141 aatcttatct gttggatggc tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa
     4201 caactgataa tctgattttc caagttccat tgtttcgcct ctttccgtta tcttttgtat
     4261 tctacctgaa ttttgcgata tggacaaata gaggttgtga aaaagctgtt ctgcatcaag
     4321 tcataagaac agccaaacaa aaaactgcga tgagcatcac cgcagtttta ttgaacttaa
     4381 ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc agctcctggg
     4441 tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt agctaccaag
     4501 actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc tgccttcaat
     4561 gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc tgcctttcct
     4621 cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc ttgagaatca
     4681 atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc gtataacgga
     4741 ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa tgtatcgctg
     4801 attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc tcttcccatg
     4861 ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata ttgtttgttc
     4921 tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac ggttagtttc
     4981 atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac attgctttct
     5041 aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag accttgcata
     5101 tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc tctttcttcc
     5161 aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt cccgcccatt
     5221 ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa aatcttagta
     5281 acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata gcctggctca
     5341 ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc tggtttccct
     5401 acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc
     5461 ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg
     5521 attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata cgtactattt
     5581 tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc catagcagct
     5641 aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac gatcagcaaa
     5701 actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg agttggagaa
     5761 gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa cccggcatgg
     5821 gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc tttctgattc
     5881 ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct ttttactaat
     5941 ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt caatttattc
     6001 ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat catctgttat
     6061 accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca ccagatgata
     6121 acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca ataccgcgct
     6181 accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga ctgcttctac
     6241 gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa aaatttgcac
     6301 ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta ctttttgact
     6361 atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta catagttgct
     6421 aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt tctaattttg
     6481 ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga gaggagtgat
     6541 cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc ttataaaggg
     6601 atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga ggcggggttg
     6661 cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa
     6721 gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc gatcattggc
     6781 gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt cacatgtagt
     6841 ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta aagagagatg
     6901 ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt actctacctt
     6961 cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg cgactaacac
     7021 acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt ttggcaaagt
     7081 ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga
     7141 ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga aactagctgc
     7201 taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc
     7261 gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct ttccagaagc
     7321 tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga tcttcccgtc
     7381 aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg
     7441 tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga ttgctgcttg
     7501 gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt ttcttttatt
     7561 ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta ttgatgtatc
     7621 ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag tcatttctgt
     7681 ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt ctgtttgagc
     7741 aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg aaacagcaat
     7801 tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta ctacttttta
     7861 actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac aggctgtttg
     7921 tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca tgtcccgctt
     7981 ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc
     8041 actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt
     8101 cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag
     8161 atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc ttgatataca
     8221 taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat tgaccagaca
     8281 atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa aggatggagc
     8341 atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca acgttttctg
     8401 tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat ctcaggagca
     8461 aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc ttctgtcgct
     8521 cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat cgtatttgct
     8581 ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat gatgcccacg
     8641 atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt ttggacatcc
     8701 atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata attcacatac
     8761 ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat caatttccct
     8821 tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt ttcgatccga
     8881 accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg tgtttggtcc
     8941 gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt cgtcatcaaa
     9001 tactcgattg tatattcgat cacaatttct tcattatggg ctggcaccat gatcgtgatg
     9061 aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt gtataaaaac
     9121 acataacacc agacaccaat aaaccataca aatccgccta aaatcggata agaaacgaga
     9181 aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag
     9241 atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat
     9301 ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac
     9361 gttccgt
//
LOCUS       DADXTG010000098         9180 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.099, whole genome shotgun sequence.
ACCESSION   DADXTG010000098 DADXTG010000000
VERSION     DADXTG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.099"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1019)
                     /gene="recA"
                     /locus_tag="IWI57_002045"
     CDS             complement(<1..1019)
                     /gene="recA"
                     /locus_tag="IWI57_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBC2450177.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKG"
     gene            complement(1128..2369)
                     /locus_tag="IWI57_002046"
     CDS             complement(1128..2369)
                     /locus_tag="IWI57_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBC2450178.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2609..3187)
                     /gene="pgsA"
                     /locus_tag="IWI57_002047"
     CDS             complement(2609..3187)
                     /gene="pgsA"
                     /locus_tag="IWI57_002047"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBC2450179.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3396..4319)
                     /locus_tag="IWI57_002048"
     CDS             complement(3396..4319)
                     /locus_tag="IWI57_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450180.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5058..6344)
                     /locus_tag="IWI57_002049"
     CDS             complement(5058..6344)
                     /locus_tag="IWI57_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBC2450181.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6337..7605)
                     /locus_tag="IWI57_002050"
     CDS             complement(6337..7605)
                     /locus_tag="IWI57_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBC2450182.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7857..8318
                     /gene="mscL"
                     /locus_tag="IWI57_002051"
     CDS             7857..8318
                     /gene="mscL"
                     /locus_tag="IWI57_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBC2450183.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8377..8823)
                     /locus_tag="IWI57_002052"
     CDS             complement(8377..8823)
                     /locus_tag="IWI57_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2450184.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(8916..9161)
                     /locus_tag="IWI57_002053"
     CDS             complement(8916..9161)
                     /locus_tag="IWI57_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBC2450185.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2526 a         1995 c         1640 g         3019 t
ORIGIN      
        1 cctttttcat cttctgggac agcgacttct tcaccaatac cataagctgc tctaactaga
       61 gcagaaactt ctgccatcat ttcaggatga ttagccatat agattttagc gttttcccgt
      121 ccttgaccaa tccgatcttc tttataagaa taccaagcac cgcttttatc aacaatgtct
      181 ttttctactg ccatgtctag aagttctcct tcttgtgaga tccctaaacc atacatgaca
      241 tctacttcag cgattttgaa aggaggtgct actttgtttt ttacgacttt gatctttgta
      301 cggttaccaa caatatctgt accttgtttc aattgttctg cacgacgtac ttccaaacgg
      361 atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga tttctggatt cccaaacatc
      421 acgcctactt tttcacggat ttggttgatg aaaatagcga ttgtttttgt tttgttgatc
      481 gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag
      541 tcacccattt cgccgtcgat ttctgctcgt ggaactaaag cagcaactga atcgactact
      601 acgatatcta cagccccact tgatactaaa gcatcagcga tctctagacc ttgttctcct
      661 gtgtcaggct gtgaaagaag tagttcatcg atattcacac ctaatttttg tgcatattgc
      721 ggatctaacg catgctcagc atcaatgaaa gcggccgttc cgccattttt ttgtacttct
      781 gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt caggtccata tacttcgatg
      841 atacgtccac gggggtaacc gccgacacct aaagcgacat ctaacgcaag agagccactt
      901 ggaattgtag agatttgttg gtcgattttt tcgcccaatt tcataataga gcctttacca
      961 tagctctttt caattttttt cagtgcagca tctaaggctg ctttacgatc atctgccaat
     1021 acgataatcc tccttatatt tacaaatcag agcaacattt cattatcctt atcatatctt
     1081 ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc gcatttatca ttttcttaaa
     1141 agctgttttc tcaaaagatc aaatccttgc ataaccgcat tatatctgat agcagcacga
     1201 tctctggcaa agtgatattt ttccgaaacc acgccgtctt tagatgaaat agcgatccat
     1261 actgttcctg cagattgacc ttctaacgtg tccggtccag ccactccggt gaaagctaca
     1321 ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca tctgctccac acattctttg
     1381 ctgactgttc catattctgc taatagctcc ggatcaatct ccagaaaacc agcttttgtt
     1441 tggagcgagt aggtaacgaa tccgcctgga aatacttcag atacaccagc aatgtcgcct
     1501 aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt
     1561 tctttcagta gttccactac gacttttgct agcgaattat catcgccgta tccataaaaa
     1621 tattccccta ctcgttcttg gattttttct tctaatgcta aaagagcttg attccctgcc
     1681 tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt catttggttt ggcatatggt
     1741 gcgatcgttg gattgatctg tgtctcaatc agatctttta gctcagtgac cagtcgcgat
     1801 tcaccaatcc cataaaagcg caaaacttta gaaattagtt tttcttcaga aggaaaacgt
     1861 tcctctagta aaggacgtac ttgttccaca aacattggtt tcaactcatt aggcggaccg
     1921 ggaagaagga tatacgcatg tgtatctgtt tgataaaaag tccctaaagc aagccctgta
     1981 cgatttggta gaggaacccc gccttcgata atctgggact gttgcagatt gtttttggtc
     2041 atttttcgat gggttgtttc aaaatatgcc aataattttt tgtatccttc cgtattttgt
     2101 atgagagact taccgaggtg agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt
     2161 cctaatccgc cacataagag tatgagttca cttcttgttt cagctagagc aatcaactct
     2221 tctaaacgct gctgattatc tcctacaact gtttgataat aaacttcgat acctagatca
     2281 gctagctgtt ctgacaagaa agtggcattc gtattcacga cttgtcctag taaaagttct
     2341 gttccgacag ctattatttc tgctttcatt acttcccgct cccttatata gtatacgctg
     2401 tatttttata tttttttatt taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca
     2461 gctccttgtt ccaaataaac agtgttctcc tgccaaaacc tcggtcataa gtcacaatat
     2521 acataaaaca tgaaaagctg ttttttgaaa ttatcccttg tgactcagag ttagacggca
     2581 ggctcacaac ctctccacta aacgatactt acattgatcc tttgaacaca ccgctgtttt
     2641 tgatgaagta atccacacct gaatagatag taaagatcag acaagcataa agcatgattt
     2701 gatctactgg aataccgatc aggctgaatg ggatattatt gataaacagc aagataatag
     2761 caaccatttg tgtagccgtc ttgacttttc ccggccaagc tgcagccatg acttctccat
     2821 gttcgacaag tagtaaacgc aagcctgtga cggctaattc acgacagaca atgatcgcta
     2881 ctacccaacc aggggccttt ccttggtcaa ccaacatgat aaaagcagtc ataactagca
     2941 ttttatctgc caacgggtcc gcaaacttgc caaaattagt gacaaggcct cgcgcacggg
     3001 caatttttcc atctaaccag tctgtgatac tagctaaagc aaaaataact gcacctacta
     3061 gctgtgtgac agccagtgtg gtgtctccta cgttaaattg tccccaatct aaaggtaaac
     3121 ctacaaccaa aatgaaaatc gggatcatac aaattctaag tacagttaat ttatttggta
     3181 aattcacact atcgactcct ttcttcccta ttctacgtga ctatcagcga agtttccacc
     3241 gatattattt acttttccaa atgaatttat tcaaaggagt atttttattg aaattgatct
     3301 ttttgaatta acgaagaagg accgcgacac agatttgtca cgatccttct tttttatacc
     3361 tacaagtaaa cagatgcgta aactaattta tacgatcatt ctgcgtattg aagagtaagg
     3421 ttcacatttt ttggactctg attgttttcc cctggattaa ctggtacgga ttctccattc
     3481 gctttgatcg ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc
     3541 ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc cttgataaac ataggcattg
     3601 tctactagca cacctaacca tacacgattg acagcagtaa attcaagggt tacaggcttg
     3661 gtagcgtctg tgatatttac tgcgatggca ctgccagaat cttctcctgt agctattgcc
     3721 attttctttt cttcacttga ttttgttgtt gattctactg tagaagactc tttggtctgc
     3781 gtgctttgct ctgaggaaga ggactcctcg actgatcctt ctactttaag cgaagaagct
     3841 gtgtctccga ttattggatc agcttgacga tcttgccaag tcatatagcc cacgacaacg
     3901 acaatcgcca aagcaaccaa tcctaataaa atgacaggaa gacttgtcca aaagcgactt
     3961 ggatgcgatt cttcctgatg cattgctttt ctagaagtac ttactgtttc aggctgcgga
     4021 cgttcaggga ccgatcctgt caatttgctt ttcccatcaa aaacatcgac taaatggtct
     4081 ccatcttctc ctacagcttc agcgtattgc cgaataaatg cccgcacgta aaatttacca
     4141 ggcaattggt caaaatcatc attgtcgatt gcttccaaat aacgtttttg aatttttgta
     4201 atctgctgta attcatctaa agatattttt ttattcaggc gtgcttgccg taatttttct
     4261 ccgatggttt caaaagccac tcgttcatct tctccagtct gtttgattct ccgaagcatc
     4321 tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc
     4381 ttttttccga ataaacattg ttctcctgcc aactcctcgg tcacaagtca caatatccaa
     4441 taaatataag aagctgtttt ttgaacttgt tccttgtgac tcggagttaa acggcaggct
     4501 cacgacctct ttcttaaacg gtgttcaatc agctgttcct cggtattagc tcaaaaaccg
     4561 gcaaaatata aggagatatt tttccgtatg tttctctaat actcaggtca aaacgctgat
     4621 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
     4681 aagttaaaaa cattaagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
     4741 gctttttcac aacctcttgg tacacggtgt tccagaagta gcttcttcgg caataagccg
     4801 aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     4861 ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata taagttgatt
     4921 aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac taagaatacc
     4981 ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt taagactttt
     5041 cctcataaaa tactggttta ttttggtctc atgtaaaact cgctgaaggc ctcttcactt
     5101 ataaatgctt ccccagctgc cagtacgtct ttcatttgga tcgaatcgat gatttcagga
     5161 agatcaaata aagttcgatc gccaaataaa ctttgcgtaa attgattggc aatatattcg
     5221 atcgaattta atgattggaa atattgtcca agcattttct tcttcagcaa atcgagattt
     5281 gtttctgaca cttccggatc atcagcgaat ccaagaatga tttctttgac tttttctgct
     5341 gctttctctg gttcgtccgt atctccgctg aagtccgcaa agtgaaattc acgatccaag
     5401 ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatcgc tagatagttt
     5461 cgagaagtat tgcctaaaat catttggaac aacagattga ttgctgtttt atagcgtaac
     5521 agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac aaatttatct
     5581 cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt ttcagggaaa
     5641 taacgtacaa tttcttgctt tggagggaaa ttttttgctt cttggttttc ccgaataagt
     5701 ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt gcttggctga
     5761 taaaacgtcc gatagcacgt atacaagtct tgagctgtga ttttgtcgat actttcaacc
     5821 gtgcccgcaa tatcgatatg caatgggtgt gtaggataca agttatttag tatgccaaaa
     5881 aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccgat gattcctttt
     5941 tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat cagagtcgtc
     6001 agattctttt ctacctgatc cgtcgtagaa aacagatagc tggttttcgt aaaacttgtg
     6061 aaagcattgg cggaagctcc ttgtttccca aataattgaa agacatcacc atcttctttt
     6121 tcaaacaact tatgttctaa aaaatgggca attccatctg gaactttcac cttttctttt
     6181 tcaccatagg gaataaattc gttatcgatc gaaccataat tcgtactgaa caaaccatat
     6241 gtcttgtgat agtcattttt aggcaacaaa tacacggtca aaccgtttgg cagtacttca
     6301 tgataaagtg tttcattgat ttgctcatat tctgttttat tcatttgcat ttcctcctgc
     6361 taaaaagaag attgcctgca attcgatttg ctcagctact tgttgtatct ctttgatcgt
     6421 tacgctctcc atacgtttca accattcttc atcagataat tttgtttccg gcagccagct
     6481 gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca gatattggtt
     6541 tctcagcatt gcttttgtct gagacagttc caactctgtt atttcaccat ttcgcaaact
     6601 ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat cgataccggt
     6661 ttggacactc atgaatcctc ggaacgtatc cacgctacta gaggcataat aagctagact
     6721 ttctttctct ctgacattca tgaaaagttt agagtgagga aaaccgccaa ataaaccgtt
     6781 aaagaccatt agcgcaaaac gttctggttc atcgtaatac acatttgtct gataagctag
     6841 gttcagcttt gcttgaacga ttggctcacg tacttgtcgt tccttcacga tattcacttg
     6901 aggttgggta taaaacagat ctggataaac tgtttcccgc gactcaaatg gtaattgccc
     6961 aactgcttca gctgctttgt ccggatcaac atcgccgaca acaaaaatgt cgatttgatc
     7021 atttgccatc atttcttgat agtaggacgc tagcgaacta gcagtgatct tgtccaaatc
     7081 ttcgactacg cccaaccctg ggatcttttg ttccgggctg ttttcaaaat aaagttcttg
     7141 caaggcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat atagacgcag
     7201 attgttcttt tccaagtcaa acgtcaactg atcaaactga tgatttttaa tatttggata
     7261 aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat cattgacata
     7321 ttcaccattc acaaaactga tccctgcgtt tagccaatgt aaatttcctt tttttctaac
     7381 tgataatcca aagcttgcgc cataaagctc tgcaagttgc gcacttaact ttgtctgatc
     7441 cgaataattc aaactatttg tttccaacat gctgctgaga agtgaacgtt tcgtgatgac
     7501 ttcttgttgc agacgcgcag taaaacggat aaaaatccgg atcgttttgt attgttttgt
     7561 tggtaagaca tgaagattca cgccttctgc caattgatac accatattta tatgctcctt
     7621 ttcacagttg attgcccgct tactctttta atgggtcaac ctgccttttt tacttattcc
     7681 tctgcttgaa atttataagt tcaggcaatt cgccgttttt tctaaatgat gttacacttt
     7741 atagtatagc atagatgttt tttatcaaaa gaaacagacg gctaaaagcg tatattgttt
     7801 ttttattttt tatttttcga tatactaaag acaaataaaa gaagggaggg tcattcatga
     7861 tcaaagaatt caaagagttc atcatgcgtg gagatgtctt ggatcttgct gtcggcgttg
     7921 ttattggtag tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg atcacaccac
     7981 taataggttg gtttgtcgca atgatcacag gtacttctga tttagaagga gcactttcaa
     8041 ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt attagtgcta
     8101 tcattacatt tctgatcact ggttttgtct tattcttagt tgtcaaagcg gcaaaccgtg
     8161 cgaagaattt acgctccaaa gaagagcaag tggaagtgga agaaactcct actgcagaag
     8221 attatcttag tgacatacgc gatcttcttg ccaaacaagt ggcagaagac aatacacatc
     8281 cagtagagca aacaaatctt tccgatactt ctaaataata aagaatctaa aaaaaagaaa
     8341 gccgattttc agtgtcggct ttcttttttt agattcttaa cttgctactt gttccttttt
     8401 catgtcgata tgtggaatat catcttccaa atacacatct gagattgcct tgaatccaaa
     8461 cgactcataa aaagcttgta ggtattcttg tgcgccgatc acgattggtt tttctggaaa
     8521 atttttctgg atcatgttaa tcgtttcttg aacaatcttt cttcctaaac catctttacg
     8581 gttgtcctga tgaacaagga cacgtccaaa atggatactc tccgcttctt catatactcg
     8641 cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac gtatgccaag cttctccatc
     8701 aatgtcatca acttcttgat aaggacactg ttgttccaca acaaaaactg cgattcgttc
     8761 tttgacgatc gcgtaaaact ctgctgttga caattcttca aatttcttta cctgatattc
     8821 cactaagcaa acgccccctg cttttttatt cttagcatac cgattcgcac acaaaaaagc
     8881 aaacgaacct tacgaatctc aggctcgttt gctttttaaa ctgcttcagt taaaaattcg
     8941 atctcaactg tccgagtaag tacatctgaa tagctgtaag atactcgttc aaaagaattt
     9001 tcttcaggat ccaaatcaac aacgaatact gaaggatagg tttctgttaa aacgccgcgg
     9061 cgttctgttt gacgctttct tcctgtctga gcaaccaaca tgattgggct gccgatacga
     9121 ccttctaaat ctttcttgat tgaagctaaa gttgttggca tacagttcac ctctactatc
//
LOCUS       DADXTG010000099         9110 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.100, whole genome shotgun sequence.
ACCESSION   DADXTG010000099 DADXTG010000000
VERSION     DADXTG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.100"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..192
                     /locus_tag="IWI57_002054"
     CDS             <1..192
                     /locus_tag="IWI57_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450186.1"
                     /translation="GADTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVG
                     SSVWAKWRKENVNEVNQND"
     gene            185..367
                     /locus_tag="IWI57_002055"
     CDS             185..367
                     /locus_tag="IWI57_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450187.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            369..839
                     /locus_tag="IWI57_002056"
     CDS             369..839
                     /locus_tag="IWI57_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450188.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            836..1378
                     /locus_tag="IWI57_002057"
     CDS             836..1378
                     /locus_tag="IWI57_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBC2450189.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            1375..1758
                     /locus_tag="IWI57_002058"
     CDS             1375..1758
                     /locus_tag="IWI57_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450190.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            1755..1985
                     /locus_tag="IWI57_002059"
     CDS             1755..1985
                     /locus_tag="IWI57_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450191.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            1992..2195
                     /locus_tag="IWI57_002060"
     CDS             1992..2195
                     /locus_tag="IWI57_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBC2450192.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            2192..2488
                     /locus_tag="IWI57_002061"
     CDS             2192..2488
                     /locus_tag="IWI57_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450193.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            2565..2978
                     /locus_tag="IWI57_002062"
     CDS             2565..2978
                     /locus_tag="IWI57_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBC2450194.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2991..3377
                     /locus_tag="IWI57_002063"
     CDS             2991..3377
                     /locus_tag="IWI57_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450195.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            3869..3941
                     /locus_tag="IWI57_002064"
     tRNA            3869..3941
                     /locus_tag="IWI57_002064"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3902..3904,aa:Lys,seq:ttt)
     gene            4327..4587
                     /locus_tag="IWI57_002065"
     CDS             4327..4587
                     /locus_tag="IWI57_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450196.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            4630..4803
                     /locus_tag="IWI57_002066"
     CDS             4630..4803
                     /locus_tag="IWI57_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450197.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            5192..6025
                     /locus_tag="IWI57_002067"
     CDS             5192..6025
                     /locus_tag="IWI57_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBC2450198.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            6018..7433
                     /locus_tag="IWI57_002068"
     CDS             6018..7433
                     /locus_tag="IWI57_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBC2450199.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            7445..8974
                     /locus_tag="IWI57_002069"
     CDS             7445..8974
                     /locus_tag="IWI57_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBC2450200.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
BASE COUNT         3247 a         1340 c         2045 g         2478 t
ORIGIN      
        1 ggtgcagata cgatggatcg atccaagcca ttacccaaac tgaattttga atcatttacg
       61 tttgagaaat atagccatct gagagaggta ggctatagtg taaatgctat tcgaaaagca
      121 cttggggtag gaagtagtgt atgggcaaaa tggcggaaag aaaatgtaaa tgaggtaaat
      181 caaaatgatt aagctaatag gcaaagagac gctcaaggtt tactatgtag cggaaacaaa
      241 ggcagaagtc agccgttggc tattgaaaat gtttaataag actagagcca atgcttcagt
      301 aagtcagaaa gcaagtgtat cagaaacagg catgcctgaa gcaatgtgga tttcaaggag
      361 gaactgagat ggcaatgata ccgaagttta gagcgtggga tagagaaaaa gacagaatga
      421 tttatcctag cacgacagga gtttgttttg aaatgaatga tgatggtata agtgtgcttg
      481 atattgatgt agattatccc aatgactatg gttttcccaa aatagcttca atcctcatgc
      541 aatctaccac tctgaaggat aagaatggtg tggagatatt tgagggggat atagcgctta
      601 tcactcataa atcaacgaac tacgccgata cttattggca cagttacgta gaagttttta
      661 gagcaaaaaa cggagcctac agaattcgcg gtgaacacat ctacgaaaca gaattatata
      721 gtaatagaaa acaattgacg gtagcgggga atgtttataa ttcaccagaa cttttgaaga
      781 gagatcgatt tatgactact accgaaaatc cagagttatt gaaggaggaa cagcgatgaa
      841 taaacaggaa ttgattgata aatatactgc agaaatatct agattaagac cttattgccc
      901 taatagacat ttaattagcg atcaacttaa atatggtctt tataaagaaa tcctagaaga
      961 tttaaaacaa ctagacgaac cgcagaaacc agcggtgcct aagtttgtgg cggaatggat
     1021 agaatacgct aaaaagaaag gcgatagtct agctatttca ttcaagccgt ggaacctcta
     1081 cggtgttgag tatagcaaga ctgatagatg gattgaagat aatcaaggaa cgtttgctcg
     1141 tgcttggcta attggctacg aggtcgagaa agagccgttg tattatgtga agttgccagt
     1201 tgtgtatttt aatcattggg atttagagac gtatctaatg aaagatgata gaggaaatat
     1261 aacaattgca gacaacaacg attttgatga tatgaaattt acggaatcag aaataaaagc
     1321 aattgatgaa agatactggc catttgctgt gccagtggaa gaggtggcgg aaggatgagc
     1381 aatcttaaag ttggagatat tgttttttac aaaggtgacg aaggcatcat ccgagaagtt
     1441 atgacggatg gaaaagaaaa tttttatagg gtagatgtca gcagaaaatc tatactctac
     1501 ctgtatgaag acgaattaga tttaggtact caaaaactca acgacaacca gaaaatggtg
     1561 tttgattatc ttaaatcaaa cgttgaacaa ggtggcaaat cagttatgta ttcgatattc
     1621 cttctaggtg attgggactc tcgaattgga gcagcaagaa acgttgatat agcttactgt
     1681 ggattaaatg gtaagcagga agcagaagtc ctatcagcat tcgctcaatg gggattagaa
     1741 caggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc cagtcaatta
     1801 ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc atcagacaaa
     1861 gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg actgattctg
     1921 tggtagagaa ccatttaaaa gaatttcgat caagtataga cgacactttc agattaggga
     1981 gttgattgac aatgaacaac agacatcgca gaataacaaa actaagaaaa caggaactga
     2041 atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa acatataaag
     2101 tggcaagtca gtgtgttgct gatgcgagta atgctattcg taagtttggg atttcgatat
     2161 taaatgatga tcgtaaatgg gaggaaatga gatgaaacta aaagacggat tttacgctag
     2221 tagtcacggc atcggcggtt taatgctaga tatgccgaca aagaatccta aaacacgtaa
     2281 gaaaccaaaa gtcaaagtcg gtgacatggt tcgctgcgaa gcagaagggt tcatctatcc
     2341 atttcgtgga tatgtagaac acgtctataa tcactcagca atcattcgta ttgaaaacac
     2401 gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtgg tgagattggt
     2461 ggatattgaa ctaatcaatg acaaataaaa aagccggatc gctccgactg attcaataaa
     2521 ttccacaagt ttattatatc acataaaagg ggcggtttga cttgatgcaa ttgttacgag
     2581 aggtagcttt caaacagaca agatgtaatg cgagagatgt gctgaagaac tttcggcgtt
     2641 tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt acggacatgc
     2701 cgaaggcacc gaagcacggc aataaggcag aagacgcgat cattcagatg atggatatag
     2761 aagcagagag agacgcgatt ttagcggctt tgatggcgct tagtctgatt agtcgtcaga
     2821 tactctacta cagcttttgt gtgccagata gcttctcaaa ctacaggatt agccgtgaag
     2881 tgggttattc agaaagaagc atacaacgga tgaagtcgga agctctgata gagtttgcag
     2941 aagcatataa acatgggaaa ataattgctt ataaatagga ggactatata atgtggaaag
     3001 attatgtatc attgaaagag ttgaaaaaag atcttatttt caaaaaaatc gtagaatggt
     3061 cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt agcgaatcag
     3121 attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca gtgattacag
     3181 atataaagat ttttgataaa ggtaaccgtc tctataatgg cgatggacat agttcttatg
     3241 ccgaagtcgt tgtttatcat aatcgaaatg agattgctaa agcggaatgt acagcaaacg
     3301 atgggaatgg aggctactat tacagtgtct gtgctttaaa agtcaaagaa aaactttgtg
     3361 tagtgactga tgcataaaaa atggcggttt tttggcggaa tgatggcggt ttttagctat
     3421 ttaccagtga tattatggta gtgtcgaaag attagtgata ggtctgagac aaaataaaat
     3481 gtaagggagg aaatctccct catcgtttaa ttaagcttcg atagacagca gcggaaatat
     3541 taagaataag gatgtgaatt ttaactcctt ctaaattgtt cttattatct atcatccgtt
     3601 gctgtctatt gttattatgt cactgtggcg gaaagggtag acgcttaaaa ataaggtcaa
     3661 tacgtcgagg gatagcctta acgttttatg atttgaccat gcaaggttcg attccttgcc
     3721 agcgacttag acgaaaggta gcttaaatat taaccacatc aacatcgtca gcgagttgat
     3781 aaaggtaact agcagactct aggatttaag ccagtcttac cgattagtct gttatagtag
     3841 caagcttgct agaggtagct cctctggttg acgtgcagct cagttggtag agcgcctgac
     3901 ttttaatcag gaggtcgccg gttcgaatcc ggtcacgtta ataaagaacc tacggaaaca
     3961 atcaatctta tcggatgccg attggttggc tgaattaagc agtgtgtctg tgaatcagtt
     4021 aataggcata aaatactagc acagacgtgt gccactctca ggtgtaggtt aggagagaaa
     4081 cattagcaac cgagggttgg aaatgggcgc tcaaagtaca cgagcaaggc gaggtcgata
     4141 gtaatcgatg gaatcggtgt aggttgctat tacataattg attgggttag attgagtttt
     4201 gggattcggt acaaatgaat cgtcaaatga ctcaagcaca ggatcggaaa cgtccctgcc
     4261 tgtgcattac atattagatc actcgttgag tggtcttttt attttagaaa agagaggatt
     4321 ttgaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg tgaaagaatt
     4381 gagaatgaga ttgctgacct aagtggagca gtacctgagt ttagtgtttt catagtctgg
     4441 tcatgcaaaa cactacagaa tagcaaagca ttagtcagcg ctagcttaaa aggagcaccg
     4501 tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac ttatctcaaa
     4561 aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac ataaaggagg
     4621 aatgatctca tgaaacaata taccgctaaa gatttcgagg aaatgaagcg attaaagaag
     4681 gactatgaag aagttgatat ggagctaact gttggagtca ttcaacgaag actgcgggtc
     4741 ggattagaga cagcaaaggc tatttacaat gatctaaatg ctattgaaga gaagaatggc
     4801 taatgaggaa ctactggtat atatcgctaa ctaatgaata tcctcgaacc attgatgatt
     4861 gttcagtgcg tgttgtgcgt tctgtacaaa tcaaagggaa gtactctatt gtcgaaatgg
     4921 ggagagaagc agaaccatgt gagattgatg cgtgcaagct cgtttattgc ggtcatggtt
     4981 tctatgatga accaaacatt caaaataata ttaacaagaa tttgagggat tagaatgcaa
     5041 gaaatggctt aaaaatctcc aatcaggaca aatcaagtta gacaaagttt ccgatttaaa
     5101 gatattaatt gaagcagatc taatgttgaa agatattgaa aattagaaaa caaaactcaa
     5161 cctaagaaga ttgcgaggtg gtgtgtattg aatggcaaga caacgtgatc caagacgtga
     5221 tgaagccaaa agaatttggt tagaatccaa cggagaaaag cagttaaagg aaattgcatc
     5281 tgaattaaat gtttcagatt ctcaggttag aaaatggaaa tcgcaagaca aatggagcgc
     5341 tgaattgaaa agtaacgtta ccaatggcaa aagtaacgtt actaatcaag gtggcgctcc
     5401 tattggtaat caaaatgcta aaggtaacaa aggaaatagc cgagcttctc cgccagtggg
     5461 taataaaaac gctttgaaaa caggcgagta tgaaaccata ttttttgaga cactaagtga
     5521 tgaagagaag gacatctatt ctagtctgaa tgataatcct tcttttgttt tgtctgaaga
     5581 aatacgtcta cttaagataa gacaatttcg tatgatgaag agaatccaac aagctgaagc
     5641 tggactaaat gatgaagaag tcgaacgatt gcagcagcta agaaagatta aaaatccgat
     5701 tgaaaaaaat ggtaaaaagc tagaaatcaa gcgtgaggtt atgcaagatg tgcagattag
     5761 cagaaaaaaa catcgcaaaa ttgatgatat tctttcaatt gaagattcat tgactcggat
     5821 tagcaaccag ttagctaaag ccatcaagca aatgaatgaa ctttatatga atgaatacag
     5881 aactgattta attaaagctc agactgataa gatccaagct gagacaaatg aaattggcgg
     5941 aaataattca ggtgaagaaa tagaagaatg gaaacaggca gttttaaatg ccgcaaacaa
     6001 acgggcggtg aaagaaaatg aataatgaat ttattccttt tgctgatatt ggttctgcca
     6061 ttgattatta ctatgataaa ccagtagctt tttgccaaga tattttgcat ttgaatcctg
     6121 atgaatggca agaaaatgtt ttaaatgatt tagctgaatt ttcaaaggtt tctgttcgtt
     6181 ctggtcaagg agttggaaaa acagcattag aagcaggagc aatactttgg ttcttaacgt
     6241 gtcgacccta cgctaaagta atagcaacag ctccgacaat gaagcaactt tacgatgtac
     6301 tttgggcaga ggtagctaaa tggttaaatg atagcttgat caaaaactta ctgaagtgga
     6361 caaagaccaa aatttatatg gttggtgatt cagagcgttg gtttgctacg gctagaacag
     6421 cgactaaacc agaaaatatg caaggttttc acgaggacca tatgttgatt gtggtagatg
     6481 aagcttctgg tgtgtctgat ccaattatgg aagctattct tggtacgcta tcaggttttg
     6541 ataataagct gttgatgtgt ggaaacccca ataatattga aggtgttttt tacgattccc
     6601 acaattcaga ccgtgataaa tacagagttc ataaagtatc aagctatgat agtaaacgta
     6661 caaacaaaga caatatagaa atgattctta aaaaatatgg aaaagaaagt gatgttgctc
     6721 gtgtccgtat ttttggagaa tttcccaaag gtgcgttgga ttcatttatc agtcttgaaa
     6781 cggttgaatt ggctacagaa aaacaaatta gtgattcttt agtcaataaa acaacggttg
     6841 ctcatattgg tgttgacgta gctcgatatg gtgatgattc tacgattctc tttcctagaa
     6901 ttgctaccag ggcattggag tatgagaagt attcaaaacg tagcaccatg gaaacaacag
     6961 gatatgtcat caacatggcc aagaatctaa tgagtcaata tccgagtatt gataaagtga
     7021 tgattaaagt cgatgacact ggtgtcggag gtggtgtaac cgaccgccta gaagaactta
     7081 tagaagacaa acattatcct tttgaggtgt ttggagtgaa taacggttca acatcagaag
     7141 acgattttta cgataattta ggtactcaac tatggggaaa catcaaggaa atgttagaag
     7201 aaaatatgac agcaaatctt aacggagaac agcctgttat tgaattgcct tctgatagtt
     7261 cgttaatcaa agaattaagt actcgcaaat tcaaaatgac aagtagaagt cgtatacgtt
     7321 tagaaagtaa agatgatatg aaaaagcgaa atattggtag tcccgatatt gctgacgcac
     7381 tggctttagc gttttatgag ccaccaagtc actatcaatt tattcaattt taggaggtga
     7441 gcttttgatt agtacagtat tatctttaga gagatataag aagctacgtg ttaaatatgc
     7501 aacacaaatt gaagatggaa tgtttgatcc gaatggtttt atagaagata tgaaaccatt
     7561 ttttgctgat cgcgaaagaa aatatctagc ttatactagc gaaaagaatg aaatagataa
     7621 tagaccaaag cctaacactg atattgtaaa agttaataat aaacttcatg ctggtatgta
     7681 ttcaattgtc gtagaccaag cagtcaatca ttttactggt atacctatca aatgggatta
     7741 cgatgtatcg gaacaaaaaa gaacactcat tcaaagatta aaagataaat tcttaaaaaa
     7801 tgatatagaa cttccgacag ttccagaagc ttttaacaaa ttaacaagca atcttgattc
     7861 catgagattc gcaatgcttg attctgaaac tgcaactttt caaggagctt gtggagtagc
     7921 tttccgattg ttagagcctg tggaagaaga tgatggttgg aagttaagag caagtaatat
     7981 tgaaccttgg agagcagaaa gatatggaaa tgccggaatc tatatcaaag aaaagtatga
     8041 ctcttaccag aaaaaatttt ttcaagaaat gaaagttatg acaagaaata aaattctgac
     8101 atatgcttgc tatggtgatt tgaattttgt tacaagcgga acgttcaaaa aaattgatga
     8161 gaccgataac cctttgggga cgattacctt ggcagaattt aaaaataata cgaatcgtta
     8221 ttgtgatttt gaagtagctg aggaaattgg agatgcaatt gatcgtgctt tgtctgatca
     8281 acaaaacgaa atcgaacaat ttaagcttgc gtacatgctt gttactggca caacaatgag
     8341 caaaggcact gctaaagaaa tgatgaatca gctagggatt atcaacttaa aagaccctac
     8401 tgcaaaagct gaatatgtca caaaaaattt agcaaaagat ttcaatgaat atcatatgga
     8461 tctattgaaa aaacagtttt acaccatttg taaagcaatc gattttaacg atgaggtgtt
     8521 taaatcaaac agttctggag aagctcgcaa gtggcaaatc attagcctag aagcgaaaac
     8581 aaatactaaa gagcaatatt ttagagaagg attgaaagaa tgcgcagaaa caattgctgc
     8641 ttttcttaag tttcatgaca aagtagaaat tgagcctgaa aaaattattt ttactttctc
     8701 tagatctttg ccaactgatt taagttattt agctgaagct ttacctaaac ttgcaccata
     8761 cgtatccaaa cgaactattc agagccaaat tccatttgta acagatgttg attatgaaaa
     8821 tgaaatgatg gaattggaaa gtggaagtgc ttatccagat agtgaataca attttggcgg
     8881 aggtggcaat agtgacgata gaaacgaagt attggaccaa acgtcgagaa ctggaggatc
     8941 aagcaaggct caaacaagaa aatcagacac ttaaaaaatt aactagtgta tttcctgaag
     9001 cactgaaaga gatacaagca aaactattat cacaagctga cttacacaat atcacttatc
     9061 cagaaatgat ggagttttat agtacaagta atcagaaaaa atatcgtgaa
//
LOCUS       DADXTG010000100         9097 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.101, whole genome shotgun sequence.
ACCESSION   DADXTG010000100 DADXTG010000000
VERSION     DADXTG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.101"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            325..1455
                     /locus_tag="IWI57_002070"
     CDS             325..1455
                     /locus_tag="IWI57_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBC2450201.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1547..2302
                     /locus_tag="IWI57_002071"
     CDS             1547..2302
                     /locus_tag="IWI57_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBC2450202.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2360..4405
                     /locus_tag="IWI57_002072"
     CDS             2360..4405
                     /locus_tag="IWI57_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBC2450203.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTA
                     EIKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4514..5665)
                     /locus_tag="IWI57_002073"
     CDS             complement(4514..5665)
                     /locus_tag="IWI57_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBC2450204.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5819..6349)
                     /locus_tag="IWI57_002074"
     CDS             complement(5819..6349)
                     /locus_tag="IWI57_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBC2450205.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6448..6798)
                     /locus_tag="IWI57_002075"
     CDS             complement(6448..6798)
                     /locus_tag="IWI57_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBC2450206.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6955..8484)
                     /gene="ahpF"
                     /locus_tag="IWI57_002076"
     CDS             complement(6955..8484)
                     /gene="ahpF"
                     /locus_tag="IWI57_002076"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBC2450207.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8494..9057)
                     /gene="ahpC"
                     /locus_tag="IWI57_002077"
     CDS             complement(8494..9057)
                     /gene="ahpC"
                     /locus_tag="IWI57_002077"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBC2450208.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         3020 a         1716 c         1709 g         2652 t
ORIGIN      
        1 gaatatagta ataaccttgt taggtgaggc tcctatacaa acataggctg ttgcccagaa
       61 acgtcgaaag tcgccaatgg gtaaaacaga aactgttgga ttaaggcttt ttccaatagc
      121 taagaatgtt ttctttacgt tgtatagtgc taaaactcgg acgatgagat gatgctaggt
      181 cagaattctt gttcgaaaag gtttctgacc ttttctttat atttttgtga ctgacttatt
      241 tgatgagaca aactcgctcc tcacctgaca agtggattgc tgtgtgagga ggagatgcgg
      301 tgaaaaaaga tcgattggtg gattttgggt ccttatttga ctttgtccat gatcggtctt
      361 ttagaagtat acagtgccag ttcttatcgt ttattgcaag ccgatgaaaa tacaaaaagt
      421 ctattattgc gtcaactaat tttcatattt ttgagttgga gcgtgatctt cttagctcgt
      481 tcagtcaaac tacactattt acttcaccct aaaatagcag gatacggttt agccttatcg
      541 attttctttt tagtattagt aagaataggg atattcggtg tcactgtcaa cggcgcacaa
      601 cgttggatct ctctgtttgg cattcaattc cagccttctg aactggcaaa tctttttttg
      661 attttttatt taagctggtt ttttcgtgac ggaaatagta gcccaaaaga tctaaaaaaa
      721 ccattcctga ttacagtagg tataactttt ctgattttat ttcagccaaa gattgctgga
      781 gcattgatga tcctttcgat tgcgtgggtc atattttggg cagcggcggt tccatttaaa
      841 aaagggatct atctaatcgt tactttttct gcattgctga ttggagcagc aggcggggta
      901 ttatatttag gaaataaagg ttggcttcca cagatgttta atcatgctta tgaaaggatc
      961 gcgactttaa gagattcttt tatcgatagt catggtgcgg gctatcaaat gacccattcg
     1021 ttttatgcac tatataatgg tggcatattt gggcggggat taggaaatag tatcacaaaa
     1081 aaagggtatc ttccagagac agaaacagat tttatttttt cgattattac agaagagctt
     1141 gggctaatcg gtgcattgtg tgtgcttttc ttattgttct cattatgcat gagaatcttt
     1201 tgtttaagca gtcgctgtaa aaatcagcaa gctggattgt ttcttttagg attcggaact
     1261 ttgctattcg ttcagacgat aatgaacgtg ggaagtatcg cggggctgat gccaatgact
     1321 ggtgtgccat tgccatttgt tagctacgga ggaacaagtt atctgatcct ttctttagga
     1381 atcggaatca cgttgaatat ttcttcaaag atacaggccg aagagttggc gctctatcga
     1441 cctgaaaaac aataaagaga aactgagaaa ctctcagttt gaattatcat aaagtacaat
     1501 cttttatttt tcaagcgaga ggttctttta gaaagaggaa ttcgcaatga aagtatctca
     1561 aaaaatcata ttatctattt tgttactgct tacattagta gttggctatt tttcgattga
     1621 gtttatccat ggattttcgt ctgcaaaaca gagttcaact gtgaaaaagg tagagccgaa
     1681 aagtatccct accacactaa atgtggcttt gattggttca gatgctcgtt cgaaagacga
     1741 aaacggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac aacaagcaaa
     1801 actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa tgaataagat
     1861 aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa aggaaaattt
     1921 caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg attgcgttaa
     1981 tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt tagatggcgt
     2041 atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt atgctcgatt
     2101 tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag tgatcaaagc
     2161 tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag cggttgggaa
     2221 attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt gtggtatgga
     2281 tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa
     2341 gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc
     2401 ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca
     2461 gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca
     2521 agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa
     2581 taaaagtgca ttatctgaaa aagagatctt agataaatac cagaatatat acggtgctgc
     2641 tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta
     2701 cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta
     2761 taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt
     2821 ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg
     2881 gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg
     2941 agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc
     3001 ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca
     3061 acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg
     3121 agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct
     3181 gattggttac gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag
     3241 taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac
     3301 aacaggtggg aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga
     3361 acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaac
     3421 agcttttgac agtctaggag gaaaagctgg atcaactgtt gcgacaacgc caaaaaccgg
     3481 tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat
     3541 ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt
     3601 tgcgacaggt tatgctcctg gatctacgtt taaaatgatt acagcagcaa tcggtctcga
     3661 caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga
     3721 cagttcttgg ggatcttatc aagtaacgcg tgtcagtagt gatgtatcac aagtagactt
     3781 aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg
     3841 tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc
     3901 aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct
     3961 agctgatact ggatatggac agggcgaact tctaattact cctatccagc aagcagcaat
     4021 gtattctgtt tttaccaaca atggcacact tgtctatcct aaattgattg cagatgcaga
     4081 taaagagaca aaagataaga agaatgtcat cagcgaaaca gcagtacaaa cgatcgtgcc
     4141 agatctgaga gaagttgtgc aagatgtaaa tggtacagca cattctcttt ctgctttagg
     4201 gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg atgtaaaggg
     4261 gcaagagaac agtttcttgt ttgctttcaa tcttgataac caaggttata tgatggtcag
     4321 catgttggaa aataaagaag atgatgattc agcaaccaaa cgagcatcgg aactattgca
     4381 atacctcaac caaaattatc aataaaaaat tgctttttaa gtacagtaaa aggactgtgg
     4441 cgttatcgtc acagtccttt tttcaaataa acggttttca cgatgtctct ttcacaactt
     4501 ctttttgatt tactcacact tcataaactt tttttgtaaa atatttgaga acaagtggag
     4561 aaataagtgt acttaataaa atcacgataa ctaaaggtga atagtactgt ggttcgatca
     4621 attgactttg ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc gaaatcatac
     4681 cagcaccgac catcaatgaa ctatgttgag aaaaaccggc aatctgagca ccaaaataac
     4741 ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat agaaataaag agtagttgtt
     4801 cactaaagtc agaaaaatca acttctaatc ctacactgac aaaaaatact ggtataaata
     4861 ctgcatatcc taaagcctct acattgtggt atacttcttc tttaaccttt gtctgactga
     4921 cagctatccc agcaaaaaaa gcaccaatga cagagctaag cccaacaaga tccgctacgt
     4981 aagacattcc taaacaaata actagcgata cgatgataat agaagaattt gcgtacactt
     5041 tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac taataagaaa ataaaaacaa
     5101 aataaattag ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa gcttcccccg
     5161 ttagaaaaga taggctgaaa cttagaacta aaacgactaa aatgtcatct accacagaag
     5221 cacccaaaat tgttgatcct tcttttgtat tcactacgtt tagttctttt aatacttcta
     5281 ctgaaatact gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct tcatttcccg
     5341 aaacttggaa tgctttgcct gtcaaccagc caaaaacaac cgggaagatg atcccaagca
     5401 aagcaacgaa cataccaggt cggaaatatt ttttcaaaag agataaatca ctttccagac
     5461 cagctaaaaa cattaataag atcacaccaa tctccgaaaa gtcatggact agaatatcag
     5521 gtttgaccca attcaatcca gcttggccta gcaaaacacc aacaagtaat tgtccaatca
     5581 ctgctgggat acctaatctc cgcgagatat gtgagccaat cgttgttgaa atcaatatca
     5641 ggcataaaat accaataaat tccattctca tcattccttt gtataaaata tagcccgaac
     5701 ttagcataac atacattatt gaaaaaatag gaaaatatac tcacaaaaaa accaacaatc
     5761 caaaagattt ttttggattg ttggttttta tacggtctat tttaataact aaaaatgatt
     5821 aacgtatagt catttttctt attctgatga aattgataat ctcctggatg cccatgaaaa
     5881 tcaaaataaa gccgaatatt tggaatagaa gcatcaaact tttgaatgga ttgaacaata
     5941 agacgaatcc gccaatcaac aacagaatac tactataaac actccaactt ttagcttttg
     6001 tattcatgct gaagaacaac tggaacaagc tgttgatgat aacagctaat ccaagaagaa
     6061 tcggcaggat agaaacgatt gcaggtgcaa aagcatagac gatcagagct aagatcagga
     6121 aaaaaagacc actgaacaga ccgatactcc aagaacctgt tttcctttta aaacggtaag
     6181 cctcaaaaag attcaacaac ccaagcaaaa caagataggc ggtgatcaga taaccgataa
     6241 aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc agaaataata taaacagcag
     6301 ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt ctcaaacata taagaaaacc
     6361 tcctttgact tattttaacg tgaactagtc attttaccaa attaaatgtt acaaaatgag
     6421 cgaaggagaa gaatatctct ggaaaaatca acttctgagg atcttttcca tttttttgcc
     6481 ttttgctagc tcatcgatca ttttatctaa ataacgaatt tctttcatca agttatcctc
     6541 gatgtcttct actcgtacac cacaaacaac acctgtaatc aaggaacgtg aaggattggg
     6601 gacaggggct tccgcaaaaa attgttcgaa agtcacttgt ttttgtagtt gatattctag
     6661 ttctgtttgg gaataacccg tcagccaaca aatgatttca tctacttcct cttttatacg
     6721 atcttttctc tccgcttttg ctacatacat aggataaacg cttgcaaaac ttgtcgtaaa
     6781 aatacgattt tgcgccataa tcagtcacct ttcttttttt caaattattt actacttata
     6841 gtatataaga aaaagagaag aaaaagcgat tattcaaaaa aacaaaaagg attgtgacaa
     6901 aattcttgtc accatccttt tttccgctct ctgacttttt acaatcttca tagctcaagt
     6961 gcgcatcaga taatcgaatg ctcctaaagc tgcagtagct cctgaaccca ttgaaacaat
     7021 gatttgtttg tatggactat ctgtacaatc accagctgcg aaaacgccgg ggatattcgt
     7081 tgcgccattt ttctcggtca ctatttcatt tcttggattc atttcgatcg ttccatctag
     7141 ccaatccgta ttagggagta agccgactag aatgaatact cctgaaacag ctagtgtatg
     7201 catctccatc gtacggcgat cgatatagga gagagattct acctgattcg taccgctgat
     7261 ttctttcgtc gctgcatgtt tgataacagt gacattcgca agagagacta gtttatcctg
     7321 taagatttga tctgctttta attctggtaa gaattctaga acatatacat gttttgctaa
     7381 cccagccaaa tcgattgctg cttcaacacc agaattacca ccaccgataa cagcaatctc
     7441 ttttccttta aataaagggc cgtcacagtg tgggcaataa gcaattcctt tatttttaaa
     7501 ctctttttcg cctggtacat tgatagaacg ccagtgcgca cctgtcgcga tgatggcggt
     7561 ttttgcttta agtgatatac cgttagctaa gccaatatta ataaaatgtt gctctttatt
     7621 taaggaaacg gcctgttgat tcatcataat gtcgataggg taactacgca catgactttc
     7681 tacttgctgc atcaattttg gaccttctgt gtaagaggta ccaatcatat tttcaatccc
     7741 tagtgtttca acgacttgtc cgccgaaaga atcagcgaca agtcctgtac ggattccttt
     7801 acgtgctgca tagatagctg cactactagc tgctggtcca ccgccaatca ctaatacatc
     7861 ataaggttct ttttcaaaca actcttcttc aatcaaaggg ccagtgattt tttcaacaat
     7921 ttgttcgatc gtcatgcgtc cgctactaaa ttcttctcca tttaaaaaca cagttggcac
     7981 tgccatgatt tttttatcat cgatttcttt ttggaacatc ccaccttcaa tcatcgtatg
     8041 agtaatattt ggatttaata cagctaatat attcaatgcc tgtacaacgt cggggcaatt
     8101 atgacaagtc aagctaacat aactgtcaaa atgcaatgtt tgtgttattt ttttgatttt
     8161 aagactcaaa gattcactga ttttcggtgc ccgtccacta atttgtaaca tagccagtaa
     8221 aaaagaggaa aattcatgac cgagggggat accagcaaaa acgattccac tttctttgcc
     8281 aactggattg atagaaaaac taggtgtgcg catcgaatct cttttttccc aagagatttt
     8341 aggagacatt tctgctattt ccttcgtaaa ttcagctagt ttttgactat tttcactatt
     8401 atccaaacta acagaaaaaa caataggaga ttcaatcagt gctaaatatt gttcgagatt
     8461 ttgtttagtt tctttgtcta acatatccac cacctaaatc tttccaacaa gatcaaaact
     8521 tggtttcaat gtttcgccac tttctttcca ttttgccgga caaacttcac ctgggtgggt
     8581 acgtacatat tgagcagcgc gaatcttatc aatcagtgca ctagcgtcac gaccgattcc
     8641 atctgcattg atttccatag cttgaacgat tccgtctgga tcaataataa atgttcctcg
     8701 ctgagctaat ccttgctctt catccaaaac atcaaaaata cgagaaatct gatgagacgg
     8761 atcaccgatc atcacgtatt cgattttacc gatagcatct gatgtatcat gccatgcttt
     8821 gtgagtaaag tgggtatcgg tagagcaaga aaagacttca acacctaaag atttcaatgt
     8881 actgtattgt tcttgtaagt cttctaattc agtaggacaa acaaaagtaa aatcagctgg
     8941 atagaagcaa agaatactcc agttccccat aagatctttt tcactgactt ctaaaaattc
     9001 tccttgatga taagcatgtg cattaaattc ttcgattttt ttcccaatca atgacatagt
     9061 aaaacctcca ataataatta tttatttata attattg
//
LOCUS       DADXTG010000101         8965 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.102, whole genome shotgun sequence.
ACCESSION   DADXTG010000101 DADXTG010000000
VERSION     DADXTG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.102"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            542..1222
                     /locus_tag="IWI57_002078"
     CDS             542..1222
                     /locus_tag="IWI57_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBC2450209.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            1251..2564
                     /locus_tag="IWI57_002079"
     CDS             1251..2564
                     /locus_tag="IWI57_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBC2450210.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            3058..3369
                     /locus_tag="IWI57_002080"
     CDS             3058..3369
                     /locus_tag="IWI57_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBC2450211.1"
                     /translation="MNMEATLKLEKRNKLIHFYPGGSREILCRCKYCEPERIQVKTGF
                     SFSVKEESFQVLLCISGDGQVESANGVMKLIRFVKKILYVFANEYWKMLSVERCGLTE
                     N"
     gene            3370..4137
                     /locus_tag="IWI57_002081"
     CDS             3370..4137
                     /locus_tag="IWI57_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HBC2450212.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            4164..5165
                     /locus_tag="IWI57_002082"
     CDS             4164..5165
                     /locus_tag="IWI57_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBC2450213.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            5188..6474
                     /locus_tag="IWI57_002083"
     CDS             5188..6474
                     /locus_tag="IWI57_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HBC2450214.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            6508..7161
                     /locus_tag="IWI57_002084"
     CDS             6508..7161
                     /locus_tag="IWI57_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2450215.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            7194..8399
                     /locus_tag="IWI57_002085"
     CDS             7194..8399
                     /locus_tag="IWI57_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBC2450216.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
BASE COUNT         2861 a         1304 c         2142 g         2658 t
ORIGIN      
        1 taggatgtga tgatgatagc cgtgaagttg ttcaagtatt taccaatgtc tacaacgcag
       61 aaaattagtt tgggtttttc ggattggaaa atttcaatgt ataaaatcat aaatttgctg
      121 taaatagaca gtttggggta gtaatagccc aataaattgt tcgataaact catctttggg
      181 gtgagaatag ctcaatcatt gaatttcaaa cctgcctttg gggtgggatt agaggtcaat
      241 atgtacctac ataatacttt ttggaaaatg atggtgttga gaatgtaagt acatttcaat
      301 gtgaaattct aatatagccc caaacgaggt caataaaaat gacctaaact cgtatttctc
      361 ccctaaacga ggaggagata gatatgaaaa ttgaatgtgc cggcgttgga gttggctgct
      421 tttagtagga atgaacatgg ttcgatgaga actgtttcag atagagtggt cgatgatcac
      481 aggcctttaa gtcgtttaca gatgatgaga cttgtaaaaa tagagggacg aatggaaaaa
      541 gatgattggt atagaagaaa agaaaggtgt tcagaaagac gtaacaattg atagggaaga
      601 gaatcttagg ggacatagat tctattggat tggacgcaga gcacaagata tcttcttctc
      661 ggctctagct ttgattgcat tgagccctgt gatgctaatt acagcgctcg ccattgtgat
      721 tgacgatccc ggtggcagcc cgatttttgt gcaggataga gttggaagaa atggaaagct
      781 gttcaagttt tataaattcc gatctatgtg tgtagatgct gaagcaaaat tagaagcctt
      841 gttggcacaa aacgagatgg atggtccggt cttcaagatt aaagatgacc caagaatcac
      901 gagagttggt aaatttattc gaaagacaag tatcgatgag ttacctcagc tctggaacat
      961 tcttaaaggt gatatgagta ttattggacc cagacctgct ttaccgagag aggtggaaca
     1021 atacggtgac tacgaaaggc agcggttgtt cgtacagcct ggtcttagtt gctattggca
     1081 gattgctcca catcgtaatg aactgtcctt tgaagaatgg gtggaacttg atgtgaagta
     1141 catcaatgaa agaagcttta tcacggactg gaagattatc ttcttgactt tacaggctgt
     1201 attatttgga tatggtgaat gattatatat agtattagga gttgaaatat gtgaatatta
     1261 ttttattatc gggtggtagc ggtaggcggt tatggccgct gtccaacgat attcgtagca
     1321 aacaattcat caaactgttt aaggatgaaa atggcgaata tgaatctatg attcaaagag
     1381 tttatcgcca gattacaact gttgacccag atgcaaaggt cactattgca acaagtaagt
     1441 ctcaggctag tgcaatccat aatcagttgg gagataaggc ttcgatttgt gtagaacctt
     1501 gtcgtagaga tactttccct gcaattttgt tagctgcagc atatcttcac gatgaacttg
     1561 gcgtaggtga tgatgaaagt gttgcggttt gtcctgttga tccttatgta gataattcct
     1621 attacgaagg cgttgaagcc cttgagaagt tggtgcagca gaatacggct aatttgactt
     1681 tgatgggtat tgcacctaca tatcctagtg aaaagtacgg ttatatcatc cctgaaagtt
     1741 ctaacgaagt aagtactgtt aaggagttta aggaaaaacc tgacaaagca acggcagaga
     1801 aatatttagc tcagaatgct gtttggaatg ccggtgtatt tgcgttcaaa cttggttacc
     1861 tgttggaaaa agctcacgca atggtggatt ttgaagatta tcgtgatttg tattccaagt
     1921 atgagacttt aaccaagatc agctttgact atgctgtggt cgaaaaagaa acgagcattc
     1981 aagtgatgcg ttataacggg gattggaaag atgtcggaac ttggaacatg atggctgaag
     2041 ttatggctga taatattaag gggaaagctg taatggatga tacctgtgtt aatactcaga
     2101 ttgtgaatga gttgaatatt cctattcttg ctatgggctg caaagatatg gtaattgcag
     2161 caagtggcga tggaattctg atttctgata aagagcgcag tggctatatg aagccgtatg
     2221 ttgagaaaat cagtacggaa gtaatgtttg cagaaaaatc ctggggtaca tataccgtta
     2281 tggacgttca ggaaggctcc atgactgtta aagtttctat gcaggccgga cagaagttga
     2341 gctaccacta ccacaattct cgcgatgaag tttggactgt tgtagcagga cagggtaaag
     2401 cgattgtaga tggtatgaag cagattcttc gtgcgggtga tgtggtgact attgccgcag
     2461 gatgtaaaca tacaatcgaa gccgtgacaa atctggatat tatcgaagtt cagattggtg
     2521 aaactattag cgtcgctgac aagtataaga gttctttgaa ctaaaagttc gcgaggtagt
     2581 tattatggag atactaaaat ttcaacccgt gggtaaagat tatctttggg gtggagaacg
     2641 gtttaagacg gaatttggaa aaaattgact taacaccatt aagcttatac gtgggaatgc
     2701 ttagttcatc tagacgataa ctacgtacgg aaatatcaaa tatgaaggac agaatggtaa
     2761 aaccgagttg tggtatgtat tggaagctac accggggggt gatttatgct tttgagtacg
     2821 atgtaacaga agaaatgtta cgaaatgctg tgatggacag atcacttggg aaacatttat
     2881 ataaagaaga agtacacaag ggagatcttt tttatgtcag ttcatggtat tggcgctgga
     2941 gtattagtgg ctgaaataca ggaaagctca aatgttacct atcgagttta cgattatgat
     3001 cgagtagata agaatgataa taagtgagaa ttgtactttg aaaaagcgat tcaggtaatg
     3061 aatatggaag ccactttgaa attggagaaa agaaataagc tcattcattt ttatcctggt
     3121 ggctctcgtg aaatactgtg tagatgtaaa tactgtgagc ccgaaagaat ccaagtaaag
     3181 acggggtttt cgttctcggt aaaagaggaa tcttttcaag ttcttttatg tatatcgggg
     3241 gacggacaag ttgagtcggc aaatggtgtt atgaaactca ttagatttgt aaaaaagata
     3301 ttgtatgttt ttgccaacga atattggaag atgttaagtg ttgagcgatg cggacttact
     3361 gaaaattaga tggggataga gggaggatat ctaatgtcac gaatgagatt tatgaacaca
     3421 gaaattgaca atcttacaat gggtgaagta ttggtagagg ttgaaaaaat gattcaagat
     3481 aggagatgcg gatatgttgt aactccgaat gtggatcaca ttgttcagtt ggaaacagga
     3541 aaagagttgc aagatgttta taaaaacgca tctttaattt tgacggatgg taaaccattg
     3601 atttggattt caaagtttta taagacacca ataaaagaaa aaatatcggg atccgatata
     3661 ttttctttac tgtgcaagat ggctgcagaa aagggataca caatgtattt tttaggtgcg
     3721 gcagagggcg ttgcggctaa agcggcagat aacctgatga aaagatttcc gggattaaat
     3781 gttgtaggaa catactctcc tccttatggg ttcgaaaaag acgaagatga aatgtttaaa
     3841 attgattccc aaattaaacg agcaaatcca gatatgctta ttgtgggttt gggttgccct
     3901 aaacaggaaa aatttatgtt ttataactgt gatagattgg gagtacctgt gtcttttggt
     3961 ctaggagcta gtttggactt tgaggcaggg aatgttaaaa gagcaccaaa atggatgtca
     4021 gattatggat ttgagtggct gtttagaatt actcaggacc ctaaaagaat ggcaaaacga
     4081 tatttggttg atgatttaaa aatctttaaa ttagcactaa aatatcggaa aaaataaact
     4141 atattcaatg aggacaatga agtatgatta ttacaaaaac accttttcgc atgtcttttt
     4201 ttggcggcgg aacggatatg gaagattatt ttcgagaaaa tggcggtgca gtgctttcca
     4261 ctacttttga taagtactgt tatgtgaatg tgcgtcattt gcctagattt tttgattaca
     4321 gcacagagtt atcatattct cgtactgaga gagtaacaaa tattgaagat atcgagcatc
     4381 ctgcaattcg taatgcgatg aaaatgctag atatgcatga aattcgcctt acatatgaag
     4441 cagatcttcc tgcacgttct ggtctgggta catctagttc ttttgccgta ggtatgttga
     4501 atgctttcta tgctttgaag ggcaagtacg ctgacaagaa gaaactcgtt gataaagcaa
     4561 tttatcttga aagagtcctt tgtaatgaag caggcggatg gcaagatcag attgcagcag
     4621 cttatggtgg ctttaaccga atcaatttca actcagatgg ctatgaagta ttgcctatta
     4681 ttattacgcc tgagcgtaaa aagcagctta acaacaactt gatgatgttc tttactggat
     4741 tcaccagatt ttcctctgat gttcagaagg caaataatgt cagcggcaca gaggacaaaa
     4801 gagtaagact gaaaaaaatg tatgagcttg tggatgaagc agaagcggtt ctgactgata
     4861 agaacagaga tctagatgat ttcggcagac tgcttgatct cacttggaga ttgaaaaagg
     4921 ctaccggtgg tgcgattagc actggaagta tcgatgaact ttatgaaaag ggcatggctg
     4981 ctggtgcatt gggcggtaaa cttctcggag caggtggcgg cggtttcttg gttttttatg
     5041 ttcagcctga aaaacaaaat gctgtgcgtt cggcaatgag taatttgatg tatattccgt
     5101 ttgaatttga aaacggtgga acacaggtta ttcattacac accggaaatg tatattccaa
     5161 aataaagaaa tcagcgagga taagaatatg aaagttgtta ttatggcagg cggtaaggga
     5221 actcgcatct catcggttgc aagcgatatt ccaaagccca tgattaaaat tgaaaacaaa
     5281 ccggtattgg agcacgaaat cgaatgtctg agagaccagg gattcacaga tattattctt
     5341 accgttagcc atcttggcga tatcattatg aactattttg gcgatggttc tggaatttca
     5401 ccagttactg gtcagccctt tgacgttaaa attgaatact ttttggagaa agagccatta
     5461 ggaaatgccg gtgcattgtt tcgtttaaag gataatttga ccgaagactt cctcctgctg
     5521 aatgcagatg cgatgtttga cgttgatttc aaccgatttg tagcatatca ccgtgaaaag
     5581 ggtggcttgg tgacattgtt tactcaccct aacagtcatc catatgacag cggtctaatc
     5641 attgcagata aggatggctc tgtagacaga tggcttgtaa aggaagattc ccgtccaact
     5701 tattaccgta acagagtaaa tgcgggactc catgtgatta gtcctgctgt tcttgatgtc
     5761 gagataaaca cacctaagat tgacttggac cgtcagttat taaagccgtt ggcaggaacc
     5821 ggaaaaatgt tctgctatga tagtccagaa tacgtaaaag acatgggtac ccctgatcgt
     5881 tattatgctg tctgtgaaga ttttaaggct ggaagagtga agggcaaaaa tcttcagaat
     5941 aagcagaaag ctgttttcct tgatcgtgat ggaacgatta atcaatatgt tggctttctg
     6001 cgtcatattg atgagttcga actaatcaag ggtactgaag atgcagtgaa aaagatcaat
     6061 gccgctggat atctggctat tgtaataacg aatcagccag taattgcaag aggtgaagtt
     6121 tcttttgaag aattggaaga gatccataac aaaatggaga cacttcttgg atataagggc
     6181 gcttatttgg atgcagttta ttattgcccg catcatccac ataagggata tgaggaagag
     6241 cgcccagagc taaaattcga ttgtgaatgc agaaagccaa agcccggaat gttaattcag
     6301 gcagctacgg acttcaacat agatttgagt cagtcttgga tgattggcga tggcgagaat
     6361 gatattaaag cgggccaagc cgccggctgt aagactgcat tggttggcga gagtgagtat
     6421 ggacaggata tgaccatagc aaccctgagt gaatttgcag ataagatctg ctaagggatg
     6481 ttttatcacg aaaggagagt ataaacaatg atagacaata gattacagaa acacattgat
     6541 ttgctgatta aacgctatcc cactcttgaa gtaatcaagc aggatattgt tgacggatac
     6601 ctgattatgg aggaatgcta tttaaatgat ggcaagctgc tgatagcagg aaatggcgga
     6661 tctgctgctg attccgagca tattgcgggt gagctgatga aaagctttaa gacgcctcgc
     6721 cctgttagta gggaatttgc agataaattg attgcggttg atcctgtaaa gggttctgaa
     6781 ttagctaaga atcttgaata tggtttgatg gcgattcctc tcgttgctca tgaagcgctt
     6841 tccactgcgt atattaatga tgttgatggc ctaggtgttt tcgcacagca gttgtttgga
     6901 tttggaagac ctggcgatgt attccttggt atttcgacat ccggcaatag caaaaatgtg
     6961 ataaatgcaa ctgttgttgc tagagctctt ggtatgaaag taattggctt aactggagcc
     7021 aaaggtggag aacttgcaag ggtagcagat gtttgtgtta aagctcctga aacagaaaca
     7081 tatatgattc aggagtacca tttgccaatt taccactgtt ggtgtctaat gttggaagat
     7141 aaattttttg ggaatagata aaatatctaa gcccatattg aaaggataaa attatgaatg
     7201 tactatttat aacaactcaa tttccatatc ctctagataa tggcggaaaa attggtgcgt
     7261 acaacggtat cagtgtttta tctagagact acgatgtgac ggtattgtct ttcaccgaag
     7321 aaccgcagta tgttgaagaa ggtgtcagtt tttttcgaga gaaatttaat agtgtaaaat
     7381 ttgaaaatcc tgtgatacat gatgtacaca taagaaataa tccaatgaaa ttggtagaag
     7441 ctttagcaat agggtattgt ctacgccaac cttatatagt ttcaaagttt tatgattcaa
     7501 aaatgctgca gttaatagat gaaaaatttt ctaacaatgc atattgggat atcattttta
     7561 ttgactatct gaatatgcaa agttatggtc aatacatacg ggagaagtat aaaacacaat
     7621 ttggtaagta tatcttaaaa gaccacaaca tagaatatga tttagtccta caggaagctg
     7681 aatcaagtcg aggtttaaaa aagaagttgc ttcagttaga gtataagcgt actcgaaagt
     7741 atgaagaggc cgcagtaaat agggccgatt attgctttag tgtgtgtgat gcaaatacgg
     7801 aagtactgaa aaagattaat gacaacagtt atacgatgct tccaacttat gaaatgctta
     7861 caggggtgaa aaaaaatata gagaagaaca cgatcctata tttgggtaac ctttcttgga
     7921 aatccaatat gcaaggatta gtttggtttt tagagtcaat ttatccaaat attcaacaaa
     7981 gaattccgga tgtaaaaatc acaattgtcg gtagcgggcc agttggaaca gattttagta
     8041 aatattcagg cgtggattat cgtgggtatg taaaagatat ttcacacatt tatgacgatc
     8101 aagcggtgct aattgtacct ttgttagaag gtagtggtat aagaataaaa atattagaag
     8161 cgttcaataa tgaaattgca gttgtatcta cattagttgg gtgtgatacg attggcgcaa
     8221 aagatggaag tgagttattt atagcagata acgaaaaaga ttttgcaacg gctgtagtta
     8281 gactgcttga agacatgaat ttgaatgaaa aagtaagaaa gaatgcaaaa ttattcttaa
     8341 aagaaaaata ctccttaacg gctagacaag atgagtttag tgcgataatt aacagctaat
     8401 atatatataa gcttatctga tgatgattta taggaggaaa tatatggaga tggctgagag
     8461 aaaggaaaag aaacttggaa ataagaatat catattattt tagaaaactc ctgttatttt
     8521 atgtttgtgt gattgttgcg gctatgagtt accaccttac aggcaattcc aatgtagcgt
     8581 cattaattgc tgtagtagga gtagtttatg ctattgtaaa ggcgtgtaat aacgagtatc
     8641 tggctatatg ggttatgttt gtattaggtt ctgatatatg ggggatagtg ccatatatta
     8701 acattggtag tggtgtgttg cattggtcag attttaatgt tatagtagcg atatttctct
     8761 gcttgagatt catcaaagtt aaagctagga gtagatacgg gattgtctat agtgtaatgc
     8821 ttatatttat tttgattgca tgtgtgcaat cgcattcaat ctatggacaa gatgtacata
     8881 caacgctctt aacagctaga gaattgctag ttgtaatatc cttttggccg atagtaaaaa
     8941 tagtatattc agaaagaata actaa
//
LOCUS       DADXTG010000102         8950 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.103, whole genome shotgun sequence.
ACCESSION   DADXTG010000102 DADXTG010000000
VERSION     DADXTG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.103"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..818)
                     /locus_tag="IWI57_002086"
     CDS             complement(<1..818)
                     /locus_tag="IWI57_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBC2450217.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRK"
     gene            complement(791..1471)
                     /locus_tag="IWI57_002087"
     CDS             complement(791..1471)
                     /locus_tag="IWI57_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBC2450218.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1494..2432)
                     /locus_tag="IWI57_002088"
     CDS             complement(1494..2432)
                     /locus_tag="IWI57_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBC2450219.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2677..3540)
                     /locus_tag="IWI57_002089"
     CDS             complement(2677..3540)
                     /locus_tag="IWI57_002089"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBC2450220.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3682..6255)
                     /gene="mprF"
                     /locus_tag="IWI57_002090"
     CDS             complement(3682..6255)
                     /gene="mprF"
                     /locus_tag="IWI57_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBC2450221.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6396..7202
                     /gene="proC"
                     /locus_tag="IWI57_002091"
     CDS             6396..7202
                     /gene="proC"
                     /locus_tag="IWI57_002091"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBC2450222.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7281..8837)
                     /gene="rny"
                     /locus_tag="IWI57_002092"
     CDS             complement(7281..8837)
                     /gene="rny"
                     /locus_tag="IWI57_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBC2450223.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2630 a         1992 c         1550 g         2778 t
ORIGIN      
        1 ttttttcttc tgaaggcaac caatattcgt ttgatgatat ttacaatcag gaacaaagca
       61 ataaagacta atgtgattgt tgctcctgga ggtgtatcta gataaaacga gctagttagt
      121 cctgacagca tccctagaaa tcctacacaa acactgatag caatcacgac attgaagctt
      181 ttgcctaatc gcataccgat tgctgcggga agaaccatga ttgcggatat caaaagtgca
      241 ccggcaatcg ggatcatcac agcaatagcc acaccagtaa tcacattgaa catcatcgac
      301 atcaaatgaa cgggtagccc atccacgtgg gcggtatctt catcaaaagt cagaacatac
      361 atcggccgtt tgaataaaaa gaacaatata ccgatggcaa cgaataagat tcctaaaaag
      421 atgacttgct cccatgtaat ggtcacgatc gaaccaaaca gataggattg gatacttgtt
      481 gctgaattcc ctccgctcaa attcatcaat actaacgcta gcgccaaacc accagccatt
      541 aaaatggcaa tggagatttc tgaataggtt cggtagatcg ttcgcagata ctccaatatc
      601 acagctgcta atacaacgac tagcatggtc gtaagtgtag gattaaggtt caaaaagaat
      661 cctaacgcta cccctgctaa agagacatga gaaagcgtat cagccatcag cgattgtctt
      721 ctgatcacta aaaacacacc tagcatcggg gcgattccgg caatgaagat agcggcaaga
      781 aacgctcttc tcatgaattc atatgaaagc aacgccattg agaatcctcc tttctcacca
      841 gtcggatctg tctgtccgca tactgtttga tatcttcatg atcatgggtg atcattagaa
      901 ctgatttccc atggacatgc gcactatgtc ggagaagacg ataaaattca tttctggatt
      961 cttcatccat acctgttgtt ggttcatcaa gaacaaataa gtcagggtca gtagcaaaaa
     1021 tacgcgctaa actgattcgc tgcttttgtc cgccagacaa ttcacctatt cgccgatttc
     1081 gcatatccca catccctaca gcttctaaag cacgctgtac atgttgatga tctttttcag
     1141 agagacgctt gaaccagcgg cctcttggat atcttcctga acggactaat tcgatcaccg
     1201 tactaggaaa acctgcattg aaagatgcaa cttgttgagg aatataaccg atacttaatt
     1261 tttttccttc ttgatttttc tcagcaatct ttatcattcc ttttgaaggt ttcagtaatc
     1321 ctaacgatgc tttcaccaag gttgacttgg ctgctccatt ttctccagtt aaaatgacaa
     1381 attcacccgc atctacatga taagagacat cttctagtac aggttcatca tcatagtaaa
     1441 aggtcaaatc agatatttcg atataacgca ccttacaacc tccattctaa aacttaattg
     1501 atactttctt ttaaagcttt cagatttttt tccatgacag aaatataatt ttcgcctgct
     1561 ttgatttgtt cattagtcaa gctttctaaa ggattcaaca cttctagctt cactcctgtt
     1621 tcattcgaca atgtctcagc taccttcgaa gaggcatttt cttcaaaata aatcactttg
     1681 atgttgtttt tcttcacaaa ttcttttagc tcagctaagc gacttggtgt gggttcctga
     1741 tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt attgattcgc taaataagca
     1801 aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat cttttagctc atcggagtat
     1861 ttttgatcca gtgcttcaag ctttttgata taagcatcgg cattcttcgt aaaagtttcc
     1921 tttttatctg gatatttttc gcctaattct tcagcaatcg ttctgacttc ttttatagct
     1981 aataccggat cgagccaaac atgtggatcg tattcatgtg aatgaccatg cccttcttct
     2041 gattcttcat gttcgtctgc ttcttgcgat tctaaacggt cgattccttt agctgcttcg
     2101 atcatcaagg tctgtttgct atccactgaa tcctttaacg aatccacaaa aaattccatg
     2161 tctgaactat tatacacgaa aacatctgca tcggaaattt ctgctctttc ttttgcactt
     2221 ggttcgaaat catgcggctc cgttccagca gggatcaaaa gtttcacgtt tccttcatcc
     2281 cctacgatct cttttgtaaa atcatacatc ggataaaaag tcgtaacgat cgtcaaatcc
     2341 tcttttttgt ctgcttcctt gttcgtattt ccacatgctg caaacagtaa agcacctatc
     2401 attaccgtag ctgtcaaaag atattttttc atttatatgc tcctttcaat gaacaaatcg
     2461 gaattttacg atttattaac gtgaactaat gtatcaaaat aaaaaaacac agtcaataga
     2521 aatccatcaa tttctgattt aagcatgtat attcttgatt aaatcgtaaa atggatgagt
     2581 tgctgctcat atctagccat ttcactacaa taaaaaactg aagaatacca ttttaggatt
     2641 cctcagtttt tcatcaccta ttttacttct tatattttat tgcagggttc gtactaaata
     2701 aacttcctca caaaaacctt ttaacccatt atatactctc tgcgctcttg aataatgttg
     2761 acacaaggca aaaacggttg gtccgcttcc actcatcaat gcagcatctg ctccgtactt
     2821 gatcatacgc tctttgattt gatggatgac tggatgccgc tttcccgtta tatcttctaa
     2881 actgtttccc atttttttga tcatctgctg ataatcttgc tctcggatgg cttcagcaag
     2941 tccgtggatg tcttgatgtt ggatggcttg cgtatctact tctctaaaca ctgtacgtgt
     3001 agagacactc attcttggtt tgaccaacac aacccagcac tgtggcatag aaggtaatac
     3061 ttggatttct tcccctttgc caccgacaaa tgccgtactt ccgtagacac agtaaggaac
     3121 atctgtaccg acttccaaac cgatttctgc cagttcttct aacgaacagc ccagattcca
     3181 taattggttg atccctctta gagcagctgc acagtctgta cttcctccgc ctaatccagc
     3241 tgctactgga atattcttct taacaaagat cttgacaccg gttttgatat tgtatttatt
     3301 ttttagcaat aaagcagctt gataaacatt gttccgttca tctaccggta aaaatgcttt
     3361 atttgtttct acaatgattt tgtcttctgt tatttcttca aaaaatagat gatctgccaa
     3421 gtctacgctc gacatcacca tttccaactc atgataccca tcttgccgtc tatataatac
     3481 atccagtcct aaatttatct tggctggcgc tttttcaatc aattccatcc tctcacccat
     3541 tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa ataaaagtat agcttatgtc
     3601 tgcctagtgg ttgattttgt catcgtttca acagctttca cagaattttc tcttcataat
     3661 ccaaacaagt tctagtgatt cttataattc tgtgagcact tcttctgcca aactcggaac
     3721 atttcctttt tcttttccta tcagactgag tagctgcatg gtagcaaatc cgaaattact
     3781 tcctttccta taagcaaaat aacgtccttc ccaatgatcg acatatttta atttcttttc
     3841 ataagcagac tggaaggaat agaaaaaatt accataatta tatacgatat gcatgacttt
     3901 ttcttgaaag aaagaaaaat ctgtttctcc aacattagct aatggagcta agcctagatt
     3961 ggcttccgag tagcccattt tctcgtatac aataaacaga ttggctaaca gatagtcagc
     4021 taattcctca gattctttag gtaagacccg caacaaatca tatcccgcct gcttcttcgt
     4081 catcggctga gcggtgatga agccaacgac tttttgatct ttacggacaa taccgacacc
     4141 gctagataat acatatgatt tatcaaatcg tcccacagag aagtattttt ctcgtgcacc
     4201 ttgaagccat ttttgtgaaa cttcatttac agcttgatgt aattcttccg gcagttgatc
     4261 gtaccaagta aaatggtatc cttctttcat caaacgttga tattcaagtt gctgactcag
     4321 ccgagacaag tcttctgttg ctagatcaac aatccctgct tcacctactt tcgtgaagtg
     4381 aaaacccaat tcatgaagca tcactacgta gctttctgat atcttataaa acgcaagctg
     4441 atagcctaaa caatctgctt gtttcataaa ctgattcgtt gctgcccgca tcattttagg
     4501 attgccaact ggatctccta aaacaaagca tttgtttcct ttgatctgat aaccaaaaac
     4561 gacttgatct tggtcgtctt cttggtaata aaaataactg taacctgaca agcgaagctg
     4621 atggcttgta gctgtgcctc cgtatgtttc caataaacgg tcaaaacggt ctttttcgta
     4681 ctcactgcca agcttcgtag tatcttcagc taaatattga taaagcgcca ctaaagctag
     4741 caatgagatg cccaaaccaa taagccctga gaaccacaca tcatctgaag gaaacaaaat
     4801 aaaatgattt cctgttattc gaccgactaa aaagcgtccg ctgtgatagc ctgcaattgc
     4861 gtaaacgata aacaaaaagc cgaacaagct agcatcaaag attatagctc cccaagaata
     4921 aacgaatttt gttcgataaa attcttttct ggataaccac acagctaaca gaatcaagac
     4981 atataccaat atcaactgcc aagataacgt acgtgtgatt gtatttaaaa taccaaaaga
     5041 tagtaaagca atcgtaggca gaaatgcctt tttgactttt actgaaagtg ctctagcaag
     5101 gccgagtaat aaaaagccaa ccaatagatt cactgtctga tctaaaaaat tgaatgagaa
     5161 cggcaataat acttgaaata gccgactgat tgcagacaga ttagtgattg ttgatagaag
     5221 taccatcatg atccccgcaa aatagacagc acttacgagc acgatatgcg caaccttttg
     5281 gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt ttgactcctg tatgtgtgat
     5341 gaacaataga agtcccgtca taaaaggtag aacataataa aacaaccgat aatataatag
     5401 ccaaactact gctacatctt gccgaacaga caattgactc attcctaaga tcatcaaaac
     5461 gtcaaaagtc cccattccac ctgggaccat cgtcagcatt cctatcaatg tagcaatcat
     5521 gaacatagga tagatcgaaa gcatcgaaat atctattctc atcaacttgc cgatgatcaa
     5581 aaataccgca agggctcccg tccattgtcc aaaggatgcc agtagcagac caagcaagcc
     5641 ttttggataa aactcgaaga acaatgtctt tctctttaga taagcaaaaa agaaaatgac
     5701 tggtgctaaa aaactaccgc cgaaaagcca gatccaatag cttcgaaaaa tactttctga
     5761 ctgaataaaa aagacatcaa taaatgtaag aaacgaccat acagacaaac cagtcagcat
     5821 aaatagtgcg acttttgata cagtggctaa aaccattttt ctatttgtct gctttccata
     5881 aaaactagcc cgcagacttg ctcctaccac accaccaaaa ccggcgagat tgttgatcgt
     5941 gtttgttacc cacgcagcaa gaagaaactc ttttctttcc atttttggtt ttccttgttt
     6001 ttctaagact ctgatcgtca cccaatcata caacaacatc ggagtgacac ccagcagtcc
     6061 agccagaatc atgagcagga tcgtcatgcg gctttgttgt cccatccttt gccaaatatc
     6121 ttgccaactc atgccattag caatatttgc gacttgattt gccacaaaga ctaacacgga
     6181 gccgaaaaaa atcagtttta aaatcagact atgttccttt atccattgca gcatcaagta
     6241 tcgtttattt ttcacatttt cccattccta acttgtatac ttttgtgtca taccccaatg
     6301 ttatcttatt tattttaatt tttccacttt agacctgttc tttgcttgcg atgatagaaa
     6361 aagaaagata tcctaatgct aaaggagagg ataacatgaa agtaggattt atcggcacag
     6421 ggaatatggc acaagctatt attgatggtt tattaaaaaa aggaacagtc tcgaaagaag
     6481 aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa atttgctgct gaaactggcg
     6541 tccatgcatg caaaagtaat caggaagttg catcacaatc agatatcgtt cttttagccg
     6601 tgaaaccaaa aatcattgaa aaagtattgg aagaactaag agaatatagc cagaaagtct
     6661 tattcatttc tattgcttca ggaaaatcat taatagattt agaaacagcg cttgaagatc
     6721 ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt cagcgtagga gcaggcgttt
     6781 ctgcgatttg caaaaatgaa gctgccacgg aggaacagct tcagcaagct acgatgattt
     6841 ttgaagcaat cggcagcgtc tatcctttag cagaatcaga tttcagtaca ttcattggcc
     6901 ttgctggcag ctcacccgca ttcgtttaca tgctgatcga tgcgatggga cggactggtg
     6961 tactgaatgg tttgtccaaa cagacagcca ctgagattgc tgcaaaagca attatgggtt
     7021 cttgtcaaaa attcctttca agtgataaaa atccatggga acttgtagac caagtttctt
     7081 ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga agaagcagga ttcatcccag
     7141 caatcatcaa aggaatcaat acgacgatcg agaaagatct tgagatgttg aataaaaaat
     7201 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
     7261 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
     7321 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
     7381 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
     7441 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
     7501 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
     7561 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
     7621 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
     7681 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
     7741 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
     7801 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
     7861 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
     7921 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
     7981 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
     8041 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
     8101 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
     8161 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
     8221 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
     8281 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
     8341 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
     8401 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
     8461 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
     8521 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
     8581 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
     8641 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
     8701 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
     8761 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
     8821 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
     8881 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
     8941 aaaaacatgc
//
LOCUS       DADXTG010000103         8880 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.104, whole genome shotgun sequence.
ACCESSION   DADXTG010000103 DADXTG010000000
VERSION     DADXTG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.104"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..208
                     /gene="nrdH"
                     /locus_tag="IWI57_002093"
     CDS             <1..208
                     /gene="nrdH"
                     /locus_tag="IWI57_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBC2450224.1"
                     /translation="SKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLKAQGFQS
                     VPVITSDAETVIGFRPDQLRKLAS"
     gene            367..2526
                     /gene="nrdE"
                     /locus_tag="IWI57_002094"
     CDS             367..2526
                     /gene="nrdE"
                     /locus_tag="IWI57_002094"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBC2450225.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2576..3541
                     /gene="nrdF"
                     /locus_tag="IWI57_002095"
     CDS             2576..3541
                     /gene="nrdF"
                     /locus_tag="IWI57_002095"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBC2450226.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3630..4769)
                     /locus_tag="IWI57_002096"
     CDS             complement(3630..4769)
                     /locus_tag="IWI57_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2450227.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5078..5791)
                     /locus_tag="IWI57_002097"
     CDS             complement(5078..5791)
                     /locus_tag="IWI57_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBC2450228.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6045..6746)
                     /gene="nagB"
                     /locus_tag="IWI57_002098"
     CDS             complement(6045..6746)
                     /gene="nagB"
                     /locus_tag="IWI57_002098"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBC2450229.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            6980..8512
                     /locus_tag="IWI57_002099"
     CDS             6980..8512
                     /locus_tag="IWI57_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2450230.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8703..>8880)
                     /locus_tag="IWI57_002100"
     CDS             complement(8703..>8880)
                     /locus_tag="IWI57_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2450231.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         2962 a         1735 c         1565 g         2618 t
ORIGIN      
        1 ttctaaaaat aattgtatgc aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat
       61 tgcttttgaa gaagtcaata tcgatgaaca gcctgaagca gtagactggt tgaaagcaca
      121 aggattccaa agtgttcctg tcattacttc tgatgcagaa acagttatcg gattccgtcc
      181 tgatcagttg cgtaaacttg ccagctaaca tgactaaaag aatcaaaacg tgatacatgg
      241 atttgaaggg cttgagacag caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag
      301 cttaaaaaca atgcaatgat ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc
      361 gtacgtatga gtcttaaaaa tttacaagat gtcagttatt tcaagctgaa taatgaaatc
      421 aatcggccag tcaatggtca aatccctttg aataaggata aagaagcttt agttgccttt
      481 tttgaggaaa atgtcaaacc aaatacgatg acttttccat ctattatgga aaaaatcaat
      541 tacttgatcg atcaagaata tatagaagca gaattcattc aattgtattc accatcattt
      601 attgaaaagc tatatcaatt tttggacgag caacattttt ctttcaaatc ttttatggct
      661 gcttacaaat tttattctca atatgcgttg aaaaccaacg acggttccgc ttatctggaa
      721 acttatgaag atcgagtagc cttcaatgcg ctttactttg cgaacgggga tgaagaatta
      781 gcacttgctc ttgcagacga aatgatccat caacgatatc agccagctac accttctttc
      841 ttgaatgctg gacgtaaacg tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact
      901 gacgatatga actctatcgg gcgctcaatc aactctgctt tgcaattgtc ccgtattggt
      961 ggcggcgtag ggattacttt atcaaatctt cgtgaagccg gcgctcctat caaaggatat
     1021 gagggtgctg caagtggtgt cgttccagtc atgaaattat tcgaagatag tttcagctat
     1081 tctaatcaat taggtcagcg tcaaggagct ggcgtggttt atttgaacgt tttccatcca
     1141 gacattattg ctttcctttc agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa
     1201 actctttctc ttggcgtgat cgtacctgat aaattttatg aattgacacg taaaaacgaa
     1261 gatatgtatt tatttagtcc atatagtgtc gaaaaagaat atggtgtccc attctcttat
     1321 gtaaatatta cagctgagta tgatcatcta gttgcaaatc caaacattcg caaacaaaaa
     1381 atcaaagcgc gtgatcttga aaatgaaatc tcaaaattac aacaagaatc tggctatcct
     1441 tacgtaatca atattgatac agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg
     1501 agtaatttat gttcagagat tttacaagtt caaacgcctt ctgtgattaa cggcaaacag
     1561 gaatatgaag tattaggaac agatatctca tgtaacttag gctcgacaaa tatcgtgaat
     1621 ttgatgcaaa gccctaactt tggtcaatct gtgcgtacga tgactcgtgc attgacattt
     1681 attacagatg catctgatat cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat
     1741 catacgattg gattaggtgc catgggctta cacacttatc ttgcaaaaga acaaatggaa
     1801 tatggctcag aggattcatt agactttaca aatatttact tcatgctgtt gaattattgg
     1861 acacttgttg aaagcaacaa tatcgctcgt gaaagaaaac aagttttcca taattttgaa
     1921 aaatcagctt atgctgacgg cacttatttc gaaaaatacg taactggaga attccagcca
     1981 aaatcagaaa aagtcaaagc attgtttgaa ggcattttta tcccaactgc tgaagattgg
     2041 aatgctttga aacaagcagt aatgaaagat ggactgtatc atcaaaatcg cttggcagta
     2101 gcaccaaatg gctcaatttc atatatcaac gatacgagtg caagtattca tcctatcact
     2161 cgcatgatcg aagaacgcca agagaagaaa atcggtaaaa tttattaccc agcgccttat
     2221 ctatccaatg agacgattcc gtattacact tctgcttatg atatggacat gcgtaaagta
     2281 atcgatgttt atgcaacggc acaacaacac gtcgatcaag gcatgagttt gactttattc
     2341 atgcgttctg aaatcccaga aggcatttat gaatggaaga caacaacgaa gcaaacgaca
     2401 cgtgatttga atattttaag acattacgct ttccacaaag gaatcaaatc catttattac
     2461 gtacgtacct ttactgatga tgcagaagaa atcggaagca atcaatgtga aagctgcgtg
     2521 atctaatatc atagatagat tcgcttattc atattttaat taaaggagca ttctcatgtc
     2581 agaaacatat tatgaagcaa taaactggaa tgaaattgaa gatatcattg ataaatccac
     2641 ttgggagaag ctgacagagc aattttggct agatacacgt atcccacttt caaatgacct
     2701 agatgattgg cgtacattgt ctcaattgga aaaagataca gtaggacatg tatttggcgg
     2761 tttgacactg ctagatactg tccaatctga aagtggcatg gatcagttgc gtaaagatgt
     2821 ccgcactccc catgaagaag ccgttttgaa caatattcaa ttcatggaat ccgttcatgc
     2881 caaaagttat tcttctattt ttagtacact gaatacgaaa aaagaaatcg aagaaatttt
     2941 tgactggaca aatacgaatc cttatctgca aaaaaaagct gagcgtatca atgaaatcta
     3001 caaacacggt acaccgctag aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt
     3061 ctattcaggt ttttatactc ctctttatta tttaggaaac aacaaattgg ctaacgtagc
     3121 agagatcatc aaactaatca tccgagatga atctgtacat ggaacctaca tcggttacaa
     3181 attccaatta ggattcaacg agttacctga agcagaacaa gaaacgttaa aagactggat
     3241 gtacaatctt ctttatgagc tttacgaaaa cgaagaacgt tatacagaag aattatacga
     3301 tccaatcggc tggacagaag aagtcaagac attcctacgc tataatgcca acaaagcatt
     3361 gatgaaccta gggatggatc cactctttcc agatactgcc aatgatgtca acccaatcgt
     3421 aatgaacggg atttcaacag gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg
     3481 ctatttgtta ggtcatgtag aagcgatgaa agacgacgat tatttgattg gattggaata
     3541 aggatttatt ttaattcaaa aatttttcga aaaataaaaa gagtttagcg tacagatatt
     3601 ttaaatgtac actgaactct tttttatgct tattcacttg tttcttccag cacttttgga
     3661 ctgtctaact tttccgaaac atgttccatt cttctcaata aatagatttt ccggatattt
     3721 aatattatcg tcttaatcac tgcatagaca ggtatgcaaa tcagcatgcc taatattcct
     3781 gcgacacttc ctgctccaat caataccaag atgatcgtca aaggatggat attcattgat
     3841 ttcccaaata acaacggctt gatcaaattt ccatctaatt gttggatcac taaaatcgac
     3901 aacgccatat acaaagcttg aaatggagag ataaacaaac cgacaatgat tgctggtacc
     3961 gctccaataa aagggccgac atacgggata atattcgtga ttccgcaaat gaccgcaagc
     4021 aacaagctat aaggctgatc gaatattctc attgccaaaa agctcattac accaataatc
     4081 aaagcatcta agatcgtact gctgatatag gcagataatg tttcgtttaa ttcttttacc
     4141 gtttggcgta actctttacg aatcgctttc ggaaagaagt gagacaacgc atcaataaat
     4201 ttgtgcccat ccttgaacat aaatagcaaa ataaatggaa cggtaaataa caaaataaaa
     4261 aattgcatca taaatgaaat gatttttgag acacttgcag ataaactgac taatatgata
     4321 ttgaacaaat tactaagaga taaatttgct gatgctaatt gttcttccaa attgaaattt
     4381 ttaaactctt cccgctgcgc caattcattt aaccatttgc ttgtttcatc tgcatacatt
     4441 ggcaagctct gggtcaattg aattgtctgt tccattaact gaggcactac attcatgaca
     4501 gttaaaacaa ctaaaatgat tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg
     4561 atccgatgct tttcgagaaa aacaacgatt ggatcgaaca tataatagat aaaaccagca
     4621 acaagcaatg gtaaaaaaat tgccgaaagc attttcaata ttggactaaa aatattcggc
     4681 atttgtaaaa taaagtacac gccgatgatc gtgagtacta gctccactgt ccagaaaaat
     4741 aattttgatt gtttaaaacg tgattgcata catctcctcc aaggtccttt gatacttcta
     4801 ttctatcatt taacaaagag ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa
     4861 aaataaacat attttaatga gtagacgagt acttaaacaa aaacacggtt tggagtagtg
     4921 acaaaaatta tactgcagtc cctttttcca aataaacggt gttcaaaagc ttgcatcttc
     4981 gtaataagcc cagccaaacg aaaaatataa ggaactattt ttatttggct gggcttatta
     5041 cttgatgtag gacatctatt ttacaacctc agatcattta ttgcgcaata ctgactaaat
     5101 gcaactttgt gatatttttg tgtacggaca actcctcgat cacatcggca taagtgagcc
     5161 cttttggaag atcaatcgta tagatatttt tgtacactcg gtagtcatca acgaatttta
     5221 cgtcaaaatt cacatcttcg attacgatat ttttctcttc aaaataatcg gtaagaaaat
     5281 ctttggtttc ttttctatgg ataaattgga tttctaactt tttagtcgta ggtacatgga
     5341 tcacgcgttt cactactgtg agtgcaatag caatcccgat aaaactgatg atcgcaatgg
     5401 aatagtaacc catgccgatg gcgatcccta atccagcaat tgcccataaa gaagcggcag
     5461 tcgtaagtcc cgtcaccgat tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga
     5521 caccactcac cacttgagca atcagccttg cttcatctga acgaatcgtt ccagcaagct
     5581 gtggatactc taaagcatct cttaatgcac cactagcgat ctcaacttgg atcaatgcaa
     5641 tgatacatgc tcccatacaa actaaaatat gcgtacgcat acctgctgga cgatttttat
     5701 attgtcgttc aaaaccgatt gctccgccaa atagcatagc gatccctaaa cgtaaaataa
     5761 tctctggaat tgtcaatacc atatcattca tctaaacacc ctccagtcca atatatcatg
     5821 tctgaaagga gcagacaaaa aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac
     5881 aaaatttttg tcatagtcct ttttatgaat aaacggtgtt caatcagctg accctcggta
     5941 ttactcaaca aacggcaaaa tatggggagc tatttttcct ttgtcttctc taatactcag
     6001 gtcaaaacgc tgatttcaca acctctttag cctactattg aaaattacaa cttacttgct
     6061 gcttcttcat cgatgatcac aacaacattg tcgtgatttt gcaatacact tgctggcaaa
     6121 tgattcgtta caggtccttc aatcatccct ttgattgcat cagctttgct ttcgccaaat
     6181 gccatcaaga cgatttcttt acttttcata attgaaccga ttcccattga aacagctttt
     6241 gttggtacat cttctgcttt atcgaagaat cgtttgtttg cattgattgt tgattctgtt
     6301 aagtcaacta ctgaagtcgt tccgtcaaat gaagcaccag gttcattgaa accgatatgt
     6361 ccattttgcc cgattcctaa gatttggata tcgattggat gtgcatcgat gatttgatca
     6421 tactttttgc atgctgcttc cagatcttct gcttttccat ctggtacaaa cgtttctttg
     6481 aatggttttt tatcaaataa ttggacatcc atgaaatgac ggtagctttg ttcatctgaa
     6541 ccgcctagtc caacgtactc atctaagttt acagaagtca tttcactgaa atccaaatcg
     6601 cttgaagtca tttctttata taaagtaact ggtgtgcttc ctgttgccaa tcctaatact
     6661 tttgcacctt tgttcatacc ttctttgatg atttcaaatg ctttttttcc gccttcttca
     6721 gcgtttttta cacgaatgat ttccatagtg attgctcctt tatattttgg tatagcccaa
     6781 ttatagcatt ctttttttct ttttacaata attagtatag accaaattgc atttttttca
     6841 ttttcttgac aataaatagg aatccgtgtt aggtttcata agatggttca atcaaacaat
     6901 ccatgttgca tgaaaaccaa gcaagtgatt cactgtggac gctttatgtc tgcagttttt
     6961 ttatgaaaaa ggagttatta tgagtatact gacattaaac aacattaccc agcaatttgg
     7021 cgacaaaatc ctctatgaag gtgtcgatct gcaattaaat gctggcgaac atcttgggct
     7081 gattggacaa aacggagccg gtaaaagtac attgattaaa atcatcactg gtgagatctt
     7141 accagatgac ggacagatcc attggcaaaa aaatattcac aaaggttacc tcgatcaata
     7201 tgtggaagtc gatgaaacat taacaatcga agaatttcta aaaactgcgt acgcaaaaga
     7261 atacgaaaag gaagaaaaaa ttgcttttct ttaccaaaca tatagcgaga ccttcgatga
     7321 ttccttgctg gaaaaagctg ggaaactcca aacggaatta gaccaaggag tgttttatca
     7381 aatcgacacc ttagttgctg agatgagcag cgggttgggg atcgatgtct taggtttaga
     7441 ttcactgttg aaaaatctaa gtggtggaca acgttcaaag gtgattttag caaaactact
     7501 tttagaaaac ccagatgttc tgatcttaga tgaaccaacc aatcacttag atgatcagca
     7561 tatccaatgg ctgacgcagt ttcttcagga ttttgacggt gcgtatatcg tgatttccca
     7621 tgaccaagaa tttcttgatc agatcaccac ccatatcgca gacatcgagt ttggaaagat
     7681 tactaagtat actggccatc taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag
     7741 ctatttgcgc caatatcatg ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg
     7801 aaaatacaaa gccggttccc gatcaacgat ggcaaaaagc cgacaaaaac aattagataa
     7861 aatcgaacgg ctgacgcctc cagcttcagc aagtaaagct gtctttgatt ttccattctc
     7921 gcctattgtc acgacattag cagtggaaac aactgattta gtcatcggat atgatcgacc
     7981 gctacttgca cccatcaatt tgaccatccg ctttggtgaa aatattgcca ttcgaggctt
     8041 caacgggatt ggaaaatcta ctttattaaa aacactgatc ggtgagattg aaaaaataag
     8101 tggcgatttc cacttcccag ataacacaaa aatcaattac ttttcacaag atctgtactg
     8161 ggaaaatcca ttagaaacac cactacaata tttaagcagt caatttccaa aagcgactat
     8221 caaagaatta cgtcgccaac tagctaaagc agggttagtt aatcaattag caagtgaacc
     8281 tctcactaca ttaagtggtg gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa
     8341 ccaaggtaat ttactgattc tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga
     8401 aagcttacaa gacgggatac agaaatttcc aggaacagta atcatcgtct ctcatgaaca
     8461 agaattttat caagatcttg tgcaacgagt cattgagatt gaaccaaaat aaacgtttga
     8521 ttgaatagtt gaaaaaaagg agctgtgaca aaagttttgt cacaactcct tcttctgaat
     8581 aaatggtggc caattagctg accttcggta ttagctcaaa aaacggcaaa atatgaggag
     8641 ctatttttat ttggctattc ttattacttg atgcagaaca gcttttcaca acctcttttc
     8701 gtctattttt tattttcgag atttctcttt cctttaaaaa caactgtttg aaagaagaac
     8761 gcacatcctt gaaaaacaag cccgctaacc gttacaccta accagccata ctgctgccac
     8821 attgtcgtcc ctagcaatga accagccgca cctccaataa agtacataaa cataaataca
//
LOCUS       DADXTG010000104         8765 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.105, whole genome shotgun sequence.
ACCESSION   DADXTG010000104 DADXTG010000000
VERSION     DADXTG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.105"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..169)
                     /locus_tag="IWI57_002101"
     CDS             complement(<1..169)
                     /locus_tag="IWI57_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBC2450232.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGN"
     gene            complement(175..1038)
                     /locus_tag="IWI57_002102"
     CDS             complement(175..1038)
                     /locus_tag="IWI57_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2450233.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1052..1948)
                     /locus_tag="IWI57_002103"
     CDS             complement(1052..1948)
                     /locus_tag="IWI57_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2450234.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2123..3412)
                     /locus_tag="IWI57_002104"
     CDS             complement(2123..3412)
                     /locus_tag="IWI57_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2450235.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3384..4901)
                     /locus_tag="IWI57_002105"
     CDS             complement(3384..4901)
                     /locus_tag="IWI57_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBC2450236.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5099..5989
                     /locus_tag="IWI57_002106"
     CDS             5099..5989
                     /locus_tag="IWI57_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBC2450237.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6062..6886)
                     /gene="nadE"
                     /locus_tag="IWI57_002107"
     CDS             complement(6062..6886)
                     /gene="nadE"
                     /locus_tag="IWI57_002107"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBC2450238.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(6898..8370)
                     /locus_tag="IWI57_002108"
     CDS             complement(6898..8370)
                     /locus_tag="IWI57_002108"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBC2450239.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         2693 a         1823 c         1435 g         2814 t
ORIGIN      
        1 tatttcctga tccctcacct ttccattgcg ccattacata aaaaacagca catgtagcag
       61 gcacgatagt aataacaggc aaacacacga ttacccagag aatacctaat aagatgtaat
      121 cagaaaccac aatcaactgt ccataaagtt tagtctcaac tattttcatc ttcctcactc
      181 tttcattcca gacatagctg ttccttgaac aaaatatttc tgtgcaaaaa tataaaccac
      241 tagtaatggc agcattgaaa taaccgttcc agccattaca ggtccataaa aagaaatgtt
      301 ctgccctgta aataaagcta accctgctgg taaagttctc atttttgaac tacttgtcaa
      361 tatcaatgga taaagcaagt cattccaact attcatcagc gtgaaaatcc ctaatgataa
      421 aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa atattaaact cacttaatcc
      481 atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta ataaaaaagg aacgcatcat
      541 gaagatacca aatgcagttg tcatacgagg aatgatcaaa cctgcataag tatcaagcat
      601 gccaagcaaa ttgacttcaa taaatagagg aatcatgaag acttggaatg ggatcatcat
      661 tgtcagcaaa acgaaataga acaaaactga ttttcctttg aaacgcatac gagcaaatgc
      721 ataccccgca agagaatcaa agaatacaga agtgatcact gtccctccag caaaaatcac
      781 tgtatttttg atgtaatcta ataaagggat cgttgtccaa accttaatat agttatctag
      841 ggtatagcta tctgcaaata ttctaggcgg aaatgagata atgtcttttt cgtgtttaaa
      901 agaagaaata atcagccaca aaaaaggaaa cgcaataacc aacaatataa ctagcatact
      961 aatcgttcga atgatttttt taccactaac tgcttttcga ctattcacag taactgtaat
     1021 cgagctttct ggtgccatac aggtttccct cctatttcat ttgtttttct ttccgtgaca
     1081 tgtacacatt agtggagata gctatagttg caacaatgat aaacaaaacc gaagacagtg
     1141 ctgatgcata gcccaattca taaggtgctg taaatcctcg actgtaaata tattgaacag
     1201 ctgtttctgt cttaaattgt gggccaccag cggttgcaac atatacttgg tcaaacactt
     1261 gcatcgaacc aatcagattc gttaaaatac aaaaacctaa ggagggaacg atctgaggta
     1321 gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc catttgagca gattcataca
     1381 aagagggaga gattccctga atagcagtca agagaatcgt catagtaaca ccaaaattct
     1441 tccaaacagt catgatggct accgtcggca tagcaagggt tggatcgcga aaaaattgag
     1501 ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc ccctacattc ggatcaagca
     1561 acatagaaaa aataatcgca attgacgtca atgaacaaat aactggaatg taaaatgtcg
     1621 aacgacaaaa acgattgaat ctggtatttt tagataatgc tactgctgct aataaaccaa
     1681 cgataatttg tgttggcgtt tcgagtacgg tgaaatataa agaatgaatc aacgaattaa
     1741 ctgctcgttc atcactaaat atacgtgtga aattttcaaa acttacaaaa tttcttgatg
     1801 taaagaaaat attcaaatct gtaaaactaa tagcaaatgt tcctaaaagc ggtatgacag
     1861 taaataataa taaaactaaa aaagatggag ttaaaaacaa atacgcagca cgatcaggac
     1921 gattccaata tttggtgaat ccgttcatat agtccacctc atctttcatt ttgataaaat
     1981 atacaatctt ggtttaaacg aatctgaata agatatccga acaatcgctg tgatttttgc
     2041 aataacacct atctgcaaat actaggtcga tcattcagat atctgtatct tgccaacagt
     2101 ctatcctgta taaagttctg tactattttt gcagtaattg attgatggca tcatctgcat
     2161 cgttcatgag tgtctcagga tcttctcctg ctaataattt ttcaatcagt ggattgatga
     2221 tatcgttatt gatcgcagag atttgggtaa aacctggtaa atatggctct gcatagtcca
     2281 tttgtttcga taattcagat acaataggat cattttttac ttcttcatct tttgctactg
     2341 aatccaagta ggcaggaaaa ccattttcga tactccattt tttaccaatt tcagttgtat
     2401 tccaatattt caaaaactca tagacagctt cttttttatt attatctgta cttgccggaa
     2461 tggcatatcc tacgccatct aaaatagctg tttttttgtc tgaatcactc ccttgtggta
     2521 aggtagtcac attataatca atctcgtttg ctttcaatcc gttatttaac catggcccat
     2581 tgatttcaat ggctagttga ccagcattca ttaaattgtc catttcggcg ccagaaatcg
     2641 attctggacc agtcttgtct acatggatca ttttttggat cgcttttaac gttttaaggt
     2701 tctcaggaga cgcaaattca gacttagtat aatcatcgtt cactaattta ccgccatttg
     2761 ctaacatcca gttatataac ggtgcagttc catccttatt gaagacaaaa cctgatacat
     2821 ttttactact atcagttaat tttggtgcca tttctgtcaa ttcttcccaa gtagtaggtg
     2881 gttgttcagt atctaatcca gcttgtttaa acaaatcatt attccaatac atatacatgc
     2941 cttgtacctg catggggaca aaatactgtt taccttctac ttcacctaat tcaatagccg
     3001 tttctttaaa atcagattta tctactccat catattccca gaaatcgtct aatggttgta
     3061 ccgctccgtt ttctacatac tgtgcaaaat ctggataatt taatgcaata aagtcaggtg
     3121 cttttttaga agaaatcgct gtaggaagtt tttcattcaa attggcccaa gtcatgatat
     3181 ccatttcaat tttgatgttt ttatcatttg ttttattaaa ttcattaacg atttcttgca
     3241 atatttcacc atcagatgca gtaaacccat tccaaaaagt caaagtcact tctttatcag
     3301 aatcagattc tgctgaactt ccgccaccgc aagctcctaa aatacctgtt gcagctacca
     3361 acaaagagcc aattaaccat tttttacctt tcattttttg ttcctccttc atctgataaa
     3421 cgacatctga ccacattcca agatagcggt ttcaataaac aattgatttt cccatcttca
     3481 gcttttacat ttgttgaatg acttggtttt acttgttcac ttttaggaag attagttgct
     3541 tttgtatcaa atccactcat ttcagaaaaa tcaatcactc cctctaatga taattcagac
     3601 aagtgaacag ttagattcat ttcttcttct ccacggttaa ctgcaaatat gacaagttct
     3661 ttgtcattta ttacggctat actttctaaa tacggcactt cttcaaaatc ttccgtcgca
     3721 tagtttgctg aacgaatttg tgggcttaac acctctcctt gtccatacac agcagcttgc
     3781 ataaatggat aatagatcgt ctgtctccaa gcttctccgt ttgtttctgt cctgattggt
     3841 gcaataacat ttaccaattg agcaagacaa gcaatcttta ctctatctgc atgtttcaat
     3901 aaagtgatta atagacagcc taatagtaat gcatcttcaa aattatagtt atcttctagt
     3961 aaaggaggag caatctgcca aggcgctgct tgctcatctt gttccgctga atgataccaa
     4021 atattccatt catcaaagga caggttgatc tgcttcttac tatgtttctt cgccttgact
     4081 gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca tgtctaagga acgtgcaaga
     4141 taatgagaaa gatcattttt ttggttgcca taatattgat gcagagacaa ataatctatt
     4201 tgatcgtacg cttgatcaag aactgttaac tcccaatcac caaatgtagg catttgccga
     4261 ttcgaacttc cgcataaaac cacctcaatt gaatcatcta cgagtttcat cgccttagcc
     4321 gtttcctctg ctaaccgtcc atattcttcc gctgttttat gtccaatctg ccaaggaccg
     4381 tccatttcat tccctaaaca ccatgtctta attccaaaag ggctctcttg accattcgaa
     4441 cgccttaaat cactccaata agttccttta ggaaaattac aatattcgac taaattacga
     4501 gctgcatcta ctccacgagt acccagattg acagccatat tgatttcggc ccccacatgc
     4561 tgacaccagt tataaaattc atgaatccca acttcattcg tttcaattgt ccgccaagct
     4621 aaatccaacc ttctaggtct atttgctatg ggcccaatac tgtcttccca acgaaatcct
     4681 gacacaaaat ttccaccagg atatctaatc atgggaatgt tcaattcttt taccaagtga
     4741 atgacgtctt ggcgaaatcc acttttatta gctgatggat gatctggctg ataaatccct
     4801 tcatatactg ctcgtcccat atgttcaata aatgaactgt acaaccgcgg atcaactttg
     4861 cctacacgct tgacactact aatcaacatt gtggcttcca taactgacct cctttaattt
     4921 atacatgaat aatacacctc gttgaaagcg ttgtcaataa tataaatgtt aaacttactt
     4981 attttttatc gattttaatt aaccaaaata ttaataatga ttaaaacaaa taatttaata
     5041 taacttttat taacaaatat gttaatatat aagaaaaacc ttttaagagg tgatatatat
     5101 gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta gtaaaacaag
     5161 gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga
     5221 acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg aagcaaacat
     5281 catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga
     5341 tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat cgatacctat
     5401 tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga tccatgattt
     5461 tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg atgcccgcat
     5521 tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa atacagagga
     5581 tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt tttcaaatga
     5641 caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa catggacgag
     5701 ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc cagataatct
     5761 taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga atatgaatgg
     5821 agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca cttggcattt
     5881 gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat ttggtaaagg
     5941 attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat atttttcttt
     6001 tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt ttgctttatt
     6061 gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt
     6121 tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct tctagataat
     6181 catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt ggtttcccat
     6241 cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc aatgcttgaa
     6301 gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg gctccaccat
     6361 caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc cctaatacgg
     6421 ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt gctttgatat
     6481 ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt acttgtgcat
     6541 caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag tcaagagcag
     6601 cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg ataaattgat
     6661 agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc aaacggcctg
     6721 ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga aacggatatt
     6781 ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt ggatcaattg
     6841 ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat tctcctccta
     6901 tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag ccattttatg
     6961 gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt ctcgtttata
     7021 ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt ttagtgcagg
     7081 taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc gagctgtaaa
     7141 gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga tagcttcttc
     7201 ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc catcaaaatt
     7261 tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg cattgcttga
     7321 taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta gcttgaatac
     7381 tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac cccatacatc
     7441 aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt cattcgaagc
     7501 atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac gcttagatat
     7561 ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt cacctaattc
     7621 tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag tgtcgactaa
     7681 gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat catttccata
     7741 agattggact aacgaatgag catgggtccc gctagctggt atcccaaata tcttcccagc
     7801 acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc cccagactgc
     7861 tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt ctccgatgac
     7921 agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca tattcaacag
     7981 tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatta atggttcatt
     8041 tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt caaaattttg
     8101 taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag caatatcgga
     8161 atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg caaaaaccgc
     8221 gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat gtaaatcagc
     8281 tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg tatgcagcgt
     8341 caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc aagttttcat
     8401 ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt ttttctgaaa
     8461 aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta aaactatcct
     8521 ttcttattgg tatttaacct aaacgagaaa gaatacttat tttattcaat gtatccgaga
     8581 cacgaagaaa ttagcgtttt ctatttttct tacttgataa aataagtaga ttttcctact
     8641 acaatcgtag ccgttaagat accgatgatt gaacaaatat aaccaaatgt ctctacaaca
     8701 taatcaatgg caaaagtttt tgaagcaata atacgtcttt ccttttttgc ttcttatccc
     8761 caaag
//
LOCUS       DADXTG010000105         8731 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.106, whole genome shotgun sequence.
ACCESSION   DADXTG010000105 DADXTG010000000
VERSION     DADXTG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.106"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IWI57_002109"
     tRNA            2..74
                     /locus_tag="IWI57_002109"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IWI57_002110"
     tRNA            78..150
                     /locus_tag="IWI57_002110"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IWI57_002111"
     tRNA            155..225
                     /locus_tag="IWI57_002111"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IWI57_002112"
     tRNA            238..311
                     /locus_tag="IWI57_002112"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IWI57_002113"
     tRNA            323..394
                     /locus_tag="IWI57_002113"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="IWI57_002114"
     CDS             660..1853
                     /locus_tag="IWI57_002114"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBC2450240.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IWI57_002115"
     CDS             1983..3455
                     /locus_tag="IWI57_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2450241.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IWI57_002116"
     CDS             3538..4326
                     /locus_tag="IWI57_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBC2450242.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IWI57_002117"
     CDS             complement(4393..5061)
                     /locus_tag="IWI57_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBC2450243.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IWI57_002118"
     CDS             5176..6888
                     /locus_tag="IWI57_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450244.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="IWI57_002119"
     CDS             6891..8663
                     /locus_tag="IWI57_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450245.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DADXTG010000106         8724 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.107, whole genome shotgun sequence.
ACCESSION   DADXTG010000106 DADXTG010000000
VERSION     DADXTG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.107"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWI57_002120"
     CDS             6..305
                     /locus_tag="IWI57_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450246.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(402..836)
                     /locus_tag="IWI57_002121"
     CDS             complement(402..836)
                     /locus_tag="IWI57_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2450247.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1068..1778)
                     /locus_tag="IWI57_002122"
     CDS             complement(1068..1778)
                     /locus_tag="IWI57_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBC2450248.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1768..2628)
                     /locus_tag="IWI57_002123"
     CDS             complement(1768..2628)
                     /locus_tag="IWI57_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBC2450249.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2633..3271)
                     /locus_tag="IWI57_002124"
     CDS             complement(2633..3271)
                     /locus_tag="IWI57_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBC2450250.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3286..3585)
                     /locus_tag="IWI57_002125"
     CDS             complement(3286..3585)
                     /locus_tag="IWI57_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBC2450251.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3628..5112)
                     /locus_tag="IWI57_002126"
     CDS             complement(3628..5112)
                     /locus_tag="IWI57_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBC2450252.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5132..5593)
                     /locus_tag="IWI57_002127"
     CDS             complement(5132..5593)
                     /locus_tag="IWI57_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2450253.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="IWI57_002128"
     CDS             complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="IWI57_002128"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBC2450254.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6896..7666
                     /locus_tag="IWI57_002129"
     CDS             6896..7666
                     /locus_tag="IWI57_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBC2450255.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7939..>8724)
                     /locus_tag="IWI57_002130"
     CDS             complement(7939..>8724)
                     /locus_tag="IWI57_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450256.1"
                     /translation="KKGLTQVQLAEGICEQATVSNIENKNMSPGINILSALCKKLNVE
                     ISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKVSNSDLLNQILFYQG
                     LLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLSKGELDFAKVYVERT
                     LKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEYNKKHKSTYALEYLL
                     YEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2641 a         1838 c         1379 g         2866 t
ORIGIN      
        1 aaaacatgga ttatactgtt gaatttgaca gtgaactact taagcaattt ccacaagaag
       61 aacatttcat gtataaagaa gcaatttccc acaaagtgac agagtatttg gagcataatt
      121 tttttcgtat aaagccagtg cgtactcgtt cggcaaaagg aatatacgag atgaaagtca
      181 aaattaataa aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt
      241 tttatattag ttcgaatttg caaaaaattg tatttgataa agaggtgagt aaaaaaataa
      301 aatagctgag cattggagga tagattgagt gatactaagt ctatcctctt gtttttctaa
      361 agaactaagc tagaattttt tcttttgtac gttattatat gttatttgtc attaccaaaa
      421 agcacctttt ttcttctagt atctatgaca taagaaatac cacccatttc ctttacttga
      481 acaaatccta ctgattcata aaattttttt gttttctcag tatcatctgt caataattcg
      541 atttggactg gtccataact agtgatcttc tccaaagctt cattcaaaag cactttgcct
      601 attccttggc gctgatacct cggatgaatg aggatatctt gaataaatgc gatcgtcgca
      661 aaatcagtga tccaacgaac gattccgata atcttccctt ccccattacg ctttatcaga
      721 gattcagatt gttcaaatgc tctagctaac cttgcagtgt cttttgtgta attcgtccaa
      781 ccaacgctct tatataattc agaaatctca tcgatcataa gggaatcaat attcattttt
      841 ttcttccttt cttatacaat tctccctcta ataatacagt tctttgacta tttaatctat
      901 cacatataac tctcctgtaa agattctctc gattccttaa aattcctctt tccttttaga
      961 atgcatactt tttgataaat aaaaaggttg tgataaattt tcttcacaac ctctgtttct
     1021 ctctatcaag tgttatcttt taattttcta cccttaccct agcatctcta ttgcccgtag
     1081 tatgcatact ttccatgttt gcgaaaataa tgtttatcta gtaaatattg cgggatttca
     1141 catatattgg ggttgatcag ctcgttttct ttggccatca gacaaatttc atctaagatc
     1201 aaactgtttt ctactgcttt cataggtgaa tccccccatg tgaacggtcc atgtccatag
     1261 acaagtactc cagggacttc gtttggatct aattttcttt ccttaaacgt ctcgacaatc
     1321 acattccctg tatgaacttc gtaagcttct cttacttctt ctttcgttaa ttggcgtgta
     1381 caaggaacct taccataaaa agcatcagca tgagtcgtgc cataagctgg caaatcgcgt
     1441 cctgcttgcg cccacatgac ggagtgcgtg gaatgagtat gagtaatagc agttatatct
     1501 tcaaaagttt gatacaaaac aacatgtgtg gggagatctg aagatggttt taagctatct
     1561 tcttctatta tatttcccga caaatctgtt accaccattt gatctgcttg catgcattca
     1621 tatctgacac cgcttggctt aatgacgatg actcctagct cacggtccac ttcactcaca
     1681 ttgccccaag tcaatttgac taatccgtat cgcggcaatg ccagatttgc ttcaaggaca
     1741 cgttgcttca ttgctgcaat tttttcttca caagacatca tagccagcct ccttcaattt
     1801 agaacttaag tagtcttttg cctgctggat ttctcttcga aagtcttggc ttttttcact
     1861 ccacatttca atcagaaatg ttccatcata atctagacgt ttcaaatgtc ttaataatcc
     1921 aagaaaatca acacaaccgc taccaaaagg aacatcacga aactggcctg aatattcttt
     1981 tgttacaggg taagtatctt ttaagtggat agcagtgata taatcgatcc ctttctcaag
     2041 ttctcgagcc ggattatttt ccggccatgc ggacaaattg cctaggtctg gatatacttg
     2101 tagaaaaggt gaatgaatct gttctttgat ttctaaaaat tttgaaatgg agttcataaa
     2161 tggatcatcc atgatttcca cggaaagaat gatgccatat ttagaagctt cctttacgct
     2221 ttcttttatt ccttcgataa agtcttctct ggaattcatt gatttctctt cataataaac
     2281 atcgtatcca gctatttgga ttactttgac gcttagatta tgcgccaaat ggattgcttt
     2341 tttcattagt tgtttcgctg cttttttctt ttctggatca gcagaaccaa acggaaatcg
     2401 ccgatgcgca cttaaacaaa tcgaatggat acgtacaccg gagctgaaca tctcttctct
     2461 cagctcagct atcttttctt cagaccaatc taatcgtgct aaccgctcat ccgtctcatc
     2521 aatcgacatc tcgataaaat cgaagtccat ttcttttaca agggaaaaac gttctttcca
     2581 tgaaatatct ttgggtaacg ctttttcata aatcccaatt tgagccattt ccttaccccc
     2641 agatacgaat gatctcatct ttgaagtttt gtgcggcctt catcggatca tttgctgctg
     2701 taataccacg accagtgata aaggtataca catcgatccc ttcaaataag tgtaatgtgt
     2761 gtggatttaa tccaccagtt acagaaacat taaagcctag ttcaatcagt tttttgatct
     2821 tcgataaatc tttttcaccc caagtttcac cagataacag cgcatcacgg ctttgatggt
     2881 agattacctg ccggataccg atgttatgcc aatccatggc ttgttcaaaa gtccaattgc
     2941 cgtagagttc tacttgcaat tcacctattt ctttttgtgc tgcctccata gttggaatcg
     3001 ttgcacaaca aatgactgtc atccagtctg ctcctgcttt tgcaacatta ctcgccactg
     3061 ttcctccagc gtctgcacat ttcgtatcag cgactagttt tttatctggg aacatactgc
     3121 gaatacagcg aatcgctttt tgtccttcct gtaagcaaag aattgtgccc acttcaataa
     3181 tatctactac atcaccaact ttcataacat ctcctaatgc atgttctaag tcattgtgat
     3241 ctaacgcaat ttgcaaattc ggcctgctca tcttagttcc ttcctttact tgtttttttg
     3301 gtaccacttt gtttcagcaa atttttcact gatttctttg actgacatca catttttgat
     3361 accgataact gtaactcctt ttttctctgc ctgagtaaac atattcaaaa aattgactgg
     3421 cgtaaaaact acatcgtatt ggccagctga actttttccc tctgaaatgg cacaatgatg
     3481 gattttggta attggtacgt ttagttcttt cattgctttt tccacacttc tcatcatcat
     3541 caagcttgtt cctgatccat ttgcacacga tactaaaatt ctcatcttaa tccctccatt
     3601 atgagtgttg tctaatcaga ttttctttta tactctctgc ttttctgcat atgtgtcata
     3661 gtcttctaca atcaaaaaat aaccatcagg atttttccga tattgtaact gcggaatagc
     3721 caataaacca atgacaacta cggcaattcc agcataacct aggactttca tgattactgt
     3781 aaacaatggc caagctgttg cccagtcgaa catcccgata taaccaccgt atgttgacat
     3841 cccaatccat gtagcgaata atgctgaacc gcctacttgt atgattcctg aaagaaacgg
     3901 gaacaggcaa gcggctttga atccacctcg gttattggca taaactgcta taacggcatt
     3961 atcaaagaac aacggaatga aaccggctat gacgatcgtt ggtgatttca ataaaatcag
     4021 taaaccaatc atcaaaaact gtcccaaagc accaaacaag aacccaatcg ttaccgcatt
     4081 tggcgaacca aagccaaaag tagcagctac gtcaattcct ggaacagctc ctggtaaaat
     4141 cgtgtttgag attccttgga aagattgagt tagttcatcg acgaatgtcc gaacacctaa
     4201 ttgcaagatc gccagatata ccgcaaaatt taaagaagtc gtcataatgt agaataagaa
     4261 actctgccct tcttgcatga attgcgcttg aatcaaataa tctttgccta gtacaatcag
     4321 aatgatccca aagaaaaata gcatgagtaa cgaagtagca accatattct cattgaaaat
     4381 cgacaagaat cctggtaatt tgatatcttc aatttttttg tcttgcttga ttgatctatt
     4441 tttcttttca tcccgctttt tcatccactc agctaatttt gcaaatataa agattccgaa
     4501 catttgctga tgtgccactg caaatccggc accttctgtc aattcctgcg tgattcctac
     4561 tgttaagtta gaagctactg tccaataaca acctaaaatc agtcccataa aaatcaatgc
     4621 ttgtatccta cctagatcag gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg
     4681 ttgaacctga acgtttccgg tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg
     4741 gactaataag atatttaaaa caaatgcaat cagtaataaa atcatcacat ctccaaaggt
     4801 acggccaaat gtctccatta atccagcatc cacagcattt tgtccaaaat aaggatctgt
     4861 aaccatggca tccaaattga accgttcctt tagtccaacc aagattggcc gaaaattatt
     4921 gaccaaacca ccagagccaa cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc
     4981 agcgatgcat tcatgaagcg ggcgtttcag caaaatatag cctaataaca cgataaaacc
     5041 aatcaggtat gctggctgcg tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa
     5101 aaattccccc atcttttttc tcctctatca attaattata ttctgccatc acaacttggt
     5161 aatcatcgat ggagttcgta gagatgagac gctcaaccaa tccatctgtc attaataatt
     5221 cggataattt ttggatattt tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa
     5281 acaaactagc cgttttctcc tcattctccc cttcactttc aaaacggatt ggctgtttca
     5341 acttggtaaa tgaaatagct gttccaaaaa caccatcact tttttctgaa gaatgcggca
     5401 ttgctacttg aggtgcgatc acaatataag gaccgtattt ttctacacaa tggatgatct
     5461 cctccacata cgtttcatca ataatttcct tatcgatcaa tacaagacag ctttcggcga
     5521 tagcttcacg ccaatccgtg atttctttat ctgggtaatg aatcaattga tggtcaaaaa
     5581 agtatttcag caagaaactc cccttctttc ttataaaaac gacggaaacg gtgtatcatt
     5641 ttcaatcgag aagacatctt caaatcctcg ataaaaatta aactcaagat catctttgtt
     5701 tgtcggatag atgaatttcc cgcctacttg ccagatataa ggtttaaatc catacttcag
     5761 gcgatccttt ttgaatttcc aaatttcagt gatctctttt ggatcagcca taaaattaga
     5821 ccaaatatcg tagtggacag gaatcactac ttttgcattc aatgactccg ccatgcgcag
     5881 catatcaaca gaagtaactt tgtctgttat ccctcgtggg ttttcaccat aggcccctaa
     5941 acagacatcg attttgtgtt cattaccatg tttggcaaat aaattggagt aatgtgaatc
     6001 tcctgcatga taaagattcc ctcctgatgt ttcgaataaa tagtttactg caatttgatc
     6061 catctcctga ggcatttttc cttttaatac ctcttctgga tcttcacaag tgaccaacgc
     6121 tgtccgatca aacgcttcta aagctactac attgatgtct ttgattttga ctgcatcacc
     6181 tggctttact gtaatcgttt tttcttcagg aattccccat ttttgccacg tgcgaaccac
     6241 ttcttttggc ccaatgaatg ttgcttcagg acaattttga tgaactgctg cggctgtatg
     6301 gatatctaga tgatcggaat ggatatgtgt aacgattaaa gcgtctacat tttttatcgc
     6361 aaaaggatcg attacgaaag gctgagtacg taaattaggc tgcatatttt gaacgccact
     6421 catacgcatc atctgatggc cttttttcat catgccattc ccatgactac gttttcccgt
     6481 cccacaccat agatcacata aaatattcgt tccttcatga cttttcagcc aaattcccgt
     6541 acagcccagc caccacatag caaaagttcc aggatccact acttctgttt cgatttcttc
     6601 attcaagtat gtgccccact caggaaatgt ttctactaac cacttttctt ttgttacttc
     6661 attgacagtt ttactcattt gcttgctccc cccatatcgt tgttccaact tacaagtaaa
     6721 tattaatgtg tatcagcatc catgaaaacc cttttatatg accgtttgtt ttttatatga
     6781 ttttctttca gtaatcaatg attgtttcag tataataaag acagatatga atgtttaaat
     6841 gaacaactag tcaaataaat ccatggatca agatgtccct caggaggcaa aagtcatgaa
     6901 aaactcactt gtcaatattg aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac
     6961 tgaacaacta tcaactttcg aattagctga atcccttgat gtatctatta gtacgatacg
     7021 aagagattta aatttattag aagaaaaaaa cgagatcata cgcaattacg gttattgttc
     7081 ttataattac aaaaaccaga ctgattttga tcaatctggt cctgaaagga ttaaacaagc
     7141 gattgccaga gaagctagta aatacattaa cgattatgat acgctgttta tcaattctag
     7201 ctcgactgcg ttgaaaacac tggattttct tcaatctaac catttgacag tcattacaaa
     7261 caacgtgaaa atcaattact ctccgcacaa agataattgt agttatattt taactggtgg
     7321 agagatgcgc tttccaaaag aagcactagt gggcgacatc gcattaaata cgatttcttc
     7381 agtcaatgca gacatttgta tcattggctg tagcggagtc gatgtagtac atggggtcac
     7441 aacaaagata ttaaatgaat caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg
     7501 taagatttta gtcgctgatc atcgaaaaat cggactgaca tcaaagttca agattgctga
     7561 tatttcagtc tttgattatt tgatcactga taaattttgt tctgaaaaag tattagagga
     7621 gatcagaaat acggggataa aaaccattca agtagattca ttttaagaac caggacgtaa
     7681 atatgtaaat aggattatca aaaaaatgaa taacaaattt atcgataaat gaaaaacacg
     7741 ctacgctcaa attgataaag gaatcccaag atgtatccat ccaatcgata ccatcataat
     7801 ctattgcttc ttttataacc gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa
     7861 aaggaggtat gacaaaagtc acacctcctt ttttcgaata aacggtattc aaatcagaac
     7921 actgatttca caaccacttt atttcaggtc gttttcaatc acctttaccg cgtaatcatt
     7981 tccgttgaaa cgcgctattt tttttgcgtc catatatctt tctaaataat catcttctcc
     8041 taattgctta tggcaagaag caatttcata aagcaagtat tccaatgcat aagtcgattt
     8101 atgtttctta ttatactcaa tacccttctc acataattgg attgctttat cataatcctc
     8161 gattttacgg taataggtgg cgatatcata atatacaatg cagaccatca gattatcaaa
     8221 atcagtctca ctcaatattt taagtgtacg ctccacataa actttcgcaa aatctaattc
     8281 tccttttgac aagtagatca ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt
     8341 ttcggtcgtt tgttccaaca cttgattaaa ataaaataat gctgcatctg gttttttcgc
     8401 ataaagatga tcaagcagtc cttgataaaa taaaatttga tttaaaagat cagaattact
     8461 tactttttcc tgatcgatcg tttgtaaaat tttataagat tcttggtatt ttgatacaga
     8521 attcaatgat tgaatctcat ttattttttt cctgattttg tcttcttctg tttctactaa
     8581 tatatcggag atctcgacat tcagcttttt gcaaagcgca cttaagatat tgatgcctgg
     8641 cgacatattc ttgttttcga tattgctgac agttgcttgt tcacaaatgc cttctgctaa
     8701 ttgtacttgt gttaacccct tttt
//
LOCUS       DADXTG010000107         8444 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.108, whole genome shotgun sequence.
ACCESSION   DADXTG010000107 DADXTG010000000
VERSION     DADXTG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.108"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            246..1925
                     /locus_tag="IWI57_002131"
     CDS             246..1925
                     /locus_tag="IWI57_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBC2450257.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            2052..2531
                     /locus_tag="IWI57_002132"
     CDS             2052..2531
                     /locus_tag="IWI57_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBC2450258.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            2750..3430
                     /locus_tag="IWI57_002133"
     CDS             2750..3430
                     /locus_tag="IWI57_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBC2450259.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            3438..4769
                     /locus_tag="IWI57_002134"
     CDS             3438..4769
                     /locus_tag="IWI57_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBC2450260.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            4784..5746
                     /locus_tag="IWI57_002135"
     CDS             4784..5746
                     /locus_tag="IWI57_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBC2450261.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            5857..6195
                     /locus_tag="IWI57_002136"
     CDS             5857..6195
                     /locus_tag="IWI57_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBC2450262.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            6274..6981
                     /locus_tag="IWI57_002137"
     CDS             6274..6981
                     /locus_tag="IWI57_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBC2450263.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(7084..>8444)
                     /locus_tag="IWI57_002138"
     CDS             complement(7084..>8444)
                     /locus_tag="IWI57_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450264.1"
                     /translation="IRKINVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLK
                     LCYLKESNRFNYLMLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRI
                     LSVKNCCTLEGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQ
                     EKNIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETE
                     TERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLE
                     NKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFS
                     KNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVK
                     ITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTK
                     RM"
BASE COUNT         2792 a         1406 c         1833 g         2413 t
ORIGIN      
        1 gaatacttat ataagtattc tggctatagc gtttcgcaaa agatccattt tgttaatgaa
       61 gtatatccaa aataaaatgc ccatccaact gtcagaacgc ggatgtgctg gtaattttcc
      121 tgctgattct ccataagttg agaaatagtg attttgcata tgcttcattg gaatggctca
      181 tatatttaga taaagaagaa aatcgacatc tttaaacttg gatgtcataa gaagaggtga
      241 agtttgtgag taccattaaa attatcccat taggcggcgt tcgtgaaaat ggaaaaaaca
      301 tgtatgtcgc agaagtgggc gaggatattt ttattttaga ttgtgggcta aaatatcctg
      361 aaaatgagtt gttggggatc gacgtcgtaa tccctgattt tacgtattta gaagaaaaca
      421 ttgatcgtgt agctggtgtc tttttaacac atggacatgc agatgcgatc ggggcacttc
      481 cttacttgtt gtcaaaaatt tctgtaccag tattcgggac agagttgacg atcgagttag
      541 caaaattgaa tgtcagtcgt aatgaagcag ccaaaggatt taaggatttc catgtggtag
      601 atgagcatac agaaatagac tttggtgaaa cagtagtgag ctttttccgt accactcata
      661 cgataccaga ttcagtgggg atcaatctga aaacaaaaga aggtagtatc gtttatacgg
      721 gagattttaa atttgaccaa acggctatcc caatgtatca aacagattat gcacgattgg
      781 cggaaatcgg caaagaagga gtcttagcct tactaagtga atcggcaaat gcagaaaatc
      841 ctcagcctgt agcttctgaa gcccaaatag cagatgaagt atttgatacg atcaaatatt
      901 gggaagggcg tatcatcgtc gcttgtgtag caagtaattt gcaacgagtg caacaagtat
      961 tggatgcagc agaaaaagca caacgaaaag ttgtcttgac tggtcaagat ttcgaacgaa
     1021 ttattcgcac agcgatgcgt ttagaaaaac ttcaattacc aagtgaagat ctgcttgtta
     1081 cgttgaaaga catgaaaaac tatgatccag aagaactgat cattttagaa actggccgaa
     1141 tgggtgaacc catcaagtct ctgcaaaaaa tggcaaacgg tacacaccgt aatgtcaaga
     1201 ttgaagaagg agatttggtt tatatcacta ctacgccaag catcgcgatg gaaaccatcg
     1261 tggcgaaaac ggaagatatc atttatcgag ctggtggaac agtgaagttg atctctgaga
     1321 atatgcgcgt ttctggccat gccaatccta atgacttgca attaatgatc aatttgatga
     1381 aaccaacata ttttgtgcca gttcaaggag agtatcgtga attagctgct catgcagatt
     1441 tggctcatgc agtagggatg ccatacaaaa atatctatat tactggacgc ggagacgtgc
     1501 tggaatataa gaacaagcgt atgagtgttg caggaactac aagtgcagaa aatgtaatga
     1561 ttgatggatt aggcataggt gatatcggaa acattgtttt acgtgatcga aaaatcttgt
     1621 cagaagacgg gattttcgta gcggttgtca ctatcagtcg gcgagaaaag aaaatcattt
     1681 caaaacctca aattacttca cggggattcg tttacgtaaa agctagccgt gatctgatga
     1741 aagaaagcag caatatgatt gaaacaatcg ttgagaaaca tttagaaagc aatgattttg
     1801 agtggagcaa attaaagcaa gacatccgag atcaattagg ccgttactta tttgaacaaa
     1861 caaaacgccg cccagtgatc ttgccagtca ttatggaagc tactcaacgt cgaaataaaa
     1921 aataaacaaa aagagtaaga tgaacctctc tatagagtgc tcgtcttgct ctttttttgc
     1981 gaaaaacaaa taatttttga atacagcatc cctcgcttta taataaacga tagaataaag
     2041 ggagtgaatc aatgatgagt caaacaaaag tcgaacgtta tttagaagaa caaaagatca
     2101 gctatcaacc acttgatttt agtcatgtgg tagaagaatc tttcacagaa gaactacaaa
     2161 aaagagggat tgatccagct ttgatatgta aaactctcgt agtgaaagga aacaaaactg
     2221 gggtgatcat cgcattgatt cctttgaacg atcacttaga ttacaaaaaa acgagacaag
     2281 tgacgaaaga tcgtaaagtt ggttttcctg gaatggattt tgtattaatg catacaggat
     2341 atccccacgg agcaaacacc cctattggaa taaaactcgc acatcctgat tattgttttt
     2401 tagcagacag ttctttaaaa gaaaaagaag aagtaattgt gtcaagcggt gaaatcggga
     2461 gaagccttcg gataaaagta gccgatttag aaaagatcgt ccatcctatt tacgctgatt
     2521 taattagtta agggacgaga agagaagcta agcagagcag agacctcaaa cttgagatta
     2581 tatctgcttg gtacttggtg catcctgtat tatctgttac aattgctttg tacatactgt
     2641 actatcaatc caatgaaaat atatttagca gctatatttt ttcattgatg tgcaagtgat
     2701 gaaatcgttt ttaataaaaa agatacagag aaaacgcgag gtgtaaaata tgaaaattac
     2761 gtatcatgga cattcctgtt taacaattga aatggaaaat ggccaacgcg tgatgattga
     2821 cccgtttatt acaggaaatc cattgataga cttaactgca gaagaagtaa agacagattg
     2881 gttgctgatt actcatggtc atagtgacca cataggagat atgattccta ttgcaaaaga
     2941 aaatcaagcc acaattatca gtattgttga gattgctgat tatgcgaaca gcagaggagt
     3001 agattcattc ggtatgaata tcggtggaaa acatgcgttt ccattcggcg tggtaaaatt
     3061 cgtacatgca cagcatagtt caagctatga agtagacggt atcgttcagt atatgggcga
     3121 gcctagcgga attattattc aagcagaagg aaaaacgatt tatcatgcag gggatactgc
     3181 ttatttcagt gatctaggat tgcttgcaga agaattcgat attgatgtcg ctttcttgcc
     3241 gattggcgat aactatacga tggggccaga agatgcagca aaggctgcgc aaacaattcg
     3301 tgccaagaaa gtagttccaa tccattacaa tactttccct gtcattgaac aagatcccga
     3361 atcttttgta gagttactgg aagatagtga aggaaaaata atgagcgtct cggaaacgct
     3421 ggaagtgtag gcagaaaatg gcgacaaaac atgatttgat tttagaatat attgaaagtt
     3481 tacctgttgg caatcgaatt tctgtaagaa gcatcgcaaa agaattgaat gtcagtgaag
     3541 gaacagcgta ccgtgcaatc aaagatgcag aaaatgtcgg actagtttca acaattcaac
     3601 gagtagggac catccgtatc gaacgcaaat taaaaaaaca tattgaacgt ttgacatttg
     3661 gcgaggtcgt caggatcatt gaaggggacg ttttaggcgg ggcggcagga ttagacaagg
     3721 tcttgaataa attcgtcatc ggcgcgatga ctaaacatgc catggaacgc tatattacgc
     3781 caggatcact aatgatcgtc ggcaaccggg cggaagtcca aaaattagca ttggaagatg
     3841 gcgccgcagt attgatcaca ggaggattca atacgactgc tgagattgca aagctagctg
     3901 atgatcttga aatgccgatc ttacgcacct cttatgatac atttacagtc gcaaccatga
     3961 tcaaccgagc gttaagcgac caattgatca aaaaagatat catgcttgtc agtgatatct
     4021 atatgccagc tgaaaagact cattatttgc agcagacaga tacgatttct gattatcagc
     4081 gtttgtcaga agaaacgcag cattcgcgtt ttccagtcgt caaccgccat catcgtttga
     4141 tggggattgt tacggcaaaa gatgttttag ggaaatcgcc aaaccagatc atcgatcggg
     4201 tgatgacgaa agaaccaatc agtgtaaaga aaacaatgag tattgcctct gttagccatc
     4261 aaatgatctg ggatgggcta gaagtcatgc cggtcgtttc cgatgacttg acattggaag
     4321 gcttgattac ccggcaagat gtaatgaaag cgatgcagct tgtccagcga caaccgcaga
     4381 ttgcagatac gatttcggat cagatatctg gaaatatcca tacgattgat actgaccgtg
     4441 aaggaaatcg tttggaaaac cctaagttca aattcgtagt agcaccacaa atggtcaatg
     4501 gcgttggaac catttctttt ggcgtactta gcgaaatcat ttctgatgtc gcacaaaaaa
     4561 caatggtgat gaaccaaaaa cgaaatatct tgattgaaca agtgaaccta cattatttgc
     4621 ggctgatcca attggaaagc gaattagaca ttcgtccacg tgtattggaa atcggcagac
     4681 gttccgctaa actggatatc gaagtgttta ttgaaaacac catcgtttcc aaagcaatcg
     4741 ttgtttgtca agtaatggaa cgttcttaga aagcaggagt gaaatgacag tacagcaaga
     4801 aatagtagat acaataaaaa aatacgataa aatcattatc cacaggcatt tgcgccctga
     4861 tcctgatgca ttaggatcac aagtaggatt agccgaacta ttacgggaaa gtttcccaga
     4921 aaaagaaata cgtcaagtag gtgaaacgat tgaagggctt cgctttttag cagaaatgca
     4981 agaagttgaa gatgtcttct accaaggagc gctagtcatc gtgactgata cagctaattc
     5041 acccagaatc agtgatcaaa gatatcagtt ggcagaaaaa ctgatcaaaa tcgaccatca
     5101 tcccaatgat gacccttatg gagatcttgt gtgggtaaat acaaaagcaa gtagctgtag
     5161 tgagatgatt gcagaatttg ctttgatgtt tcctgaatct ttaaagatga acgttaaggc
     5221 tgcacggtta ctttatgcag gaatcgtcgg agatacaggg cgctttttgt atccctccac
     5281 gacgtcccgt acattagaga ttgcttcgat attgagaaat tatccatttg atgcaagtgc
     5341 tttgaacaga gagattgaac aaatgccgat gaaagtcgct aaactttccg gttatcttta
     5401 tcaaaatatc caggtggatg aaaatggtgc aggaagaata atccttccgc aagaaattct
     5461 tgataaatat ggaatcgatg attcagagac agcttcgatc gtttcacttc ctggagtgat
     5521 tgacgaagtg ctggcttggg ggatttttgt tcagcagcct tcagggtatt accgcgtacg
     5581 tttacgttct aaggggccga tcatcaatga attggctaaa cgccatcatg gaggaggcca
     5641 tccattagca agtggtgcga atgcaaaaga tttagcagaa gtagaggaaa tctataaaga
     5701 gatccaagat atatgccaat cgtataagag cagaaaaaca gaatgatact tcgttcgaaa
     5761 acgtgccttt tgtagtaaaa atcacaaatg tatcttttca agatcttctt tttccgttac
     5821 aatagtgctc actaaagatt ttaaaggaga aagaagatga cattaccaaa ttgcccagaa
     5881 tgtggctcag agtacacata tgaagatcga ggcttgttga tttgcccaga atgcgggaac
     5941 gaatggaaca aagaagaaca agaaactgtc atagaagacg gattagtggt attagatgca
     6001 aatggaaacc gtctgttaga gggcgatagc gtgacagtga ttaaagactt gaaagtaaaa
     6061 ggtgcgtcag gtgccattaa acaaggaaca aaagtcaaga acatccgttt agtagaaggc
     6121 gatcataata tcgattgcaa ggtagaagga tttggaccga tgaaactgaa atcggaattt
     6181 gtgaaaaaga attaaagaat attcaaggtc ttttcacatt cttttcctac tactaatgat
     6241 aaaataaagt aaacgaaaag gagtgaaacg aaaatgtatg gttatggaat gtatggtttt
     6301 ttagatccga catttctttt ggtcattctt ggattagtta tttccggaat tgcgtcagcg
     6361 tacgtcaata gtactttccg aaaatacgat caagtgagaa gcaaaaacaa tgtgactggt
     6421 acacaagcag cacaatatat attacaaagt caacagataa ataatgtcgg tgttcaacaa
     6481 attgccggag atctcacaga taactacaat tcaggtaata agatgttgag tttgtctcaa
     6541 gcaactgcac agtctacttc agttgctgcg ataggtgtag cagctcatga atgcggacat
     6601 gcggtacaag atgcttcggg ttatgtccca ttgaagattc gtgcagcact tgttccagca
     6661 gctaactttg gttcgatgat ttcgttcccc ttgattatgg taggtgtatt gcttagctgg
     6721 aactctacgt taatcaatat cgggattttc gctttttctt tagcattact tttccaattg
     6781 gtgacattac cagtcgaatt taacgcgtct cggcgagcaa tccaaatttt gagtgaaggc
     6841 ggactactga cggaagaaga agtaccgatg gcaagacacg tattatttgc agcagcactg
     6901 acttatgttg cagcagcgct ttcgacattc ctgcagctgt tgcgcttgat cttattgttc
     6961 ggcggcaacc gaagagatta aaataaagaa gttgtgaaat cagcgctttg atctgagcta
     7021 ataccgagag ttagctgatt gaataccatt tatttggaat aagaggatgt gacaagaatt
     7081 ttgtcacatc ctctttgttt tgttctcata accttttttc atatacagtt catgaaccgc
     7141 ttttattaac ttgccaaata acactgtatt gtgcgttgta tccagataga aagtctccgt
     7201 ttcatctgaa attttctcat gctgatctgt aatgatcaaa tcagcatgtt ctgaatatgc
     7261 ggtaatttta acagcatctt tgcgaaataa ctgctgcaaa tggctttgaa gatgataatt
     7321 tcctgtaaac tgtttggtta tctgcaaatg gatgagtaac tgctctttcg tttctgtttg
     7381 tagtaagtgt tggatcaatt gactaaggta ttgactgatt tggtttcttt ccccattttt
     7441 tgaaaaaatt gtatcgattt tttctcttaa tttcttttcc tgtataaaag aactagaatg
     7501 attggacgtg gcttctggaa agcaaagtgt agaaaaacta ctgctggatt cacccaacag
     7561 ttcgaaaaaa acaactgcca acacaagaaa ataaaaataa attggtattt tattttctaa
     7621 atggttgaga ggaaactgtt gagttaagta agtcatcaat tgaccgactt gtcgacaaat
     7681 aaggtttttg cttgtggaaa ataaaaaacc aatctcctgt ttttgtcgaa aagagatttg
     7741 atttggaaaa aaacaataga aaaaataatt aaagtaggct ctctctgttt ctgtctcttc
     7801 acaagggcga ctcactgaaa aagaagtaaa gtattctgaa taatcatgtg tatgctgcaa
     7861 taagtccatc aattcagtga tttcagaaga agtttttgga aaatatgcct gttgtttgtt
     7921 tcgtaatatg aataaagtta acagcaagta gatatttttt tcttgttttg aaattttttc
     7981 tatttgctca tctgtactgt tcatttttag tgcatttaat tcaagtgagg aaaatggcca
     8041 atcgttacct ccaaatgtat ctgaaagaaa aaagtaaaga tagagacgga tagataactc
     8101 atttccttca agagtacagc aattttttac agataggatc cgtaaatgga atttttttaa
     8161 ggaatgattg agctgttttg tcagtttttc gagataggag ggcgatatat aaagttgttc
     8221 aagtaaatat ttttttggta cagcagtttg tggataattg attagtaaca tcaaatagtt
     8281 aaaacgattg gattctttta ggtagcataa ttttaactta gaaaaaacag ctaacttttt
     8341 agtttgatcg gtttctttgg tttggatctc tcctttatga ttagaataaa taaattcatc
     8401 aatcaataaa aattgcttga gtgatacatt tatttttcgg atca
//
LOCUS       DADXTG010000108         8435 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.109, whole genome shotgun sequence.
ACCESSION   DADXTG010000108 DADXTG010000000
VERSION     DADXTG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.109"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(124..801)
                     /gene="nth"
                     /locus_tag="IWI57_002139"
     CDS             complement(124..801)
                     /gene="nth"
                     /locus_tag="IWI57_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBC2450265.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(808..1506)
                     /locus_tag="IWI57_002140"
     CDS             complement(808..1506)
                     /locus_tag="IWI57_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBC2450266.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1774..2958
                     /locus_tag="IWI57_002141"
     CDS             1774..2958
                     /locus_tag="IWI57_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBC2450267.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2964..3407)
                     /locus_tag="IWI57_002142"
     CDS             complement(2964..3407)
                     /locus_tag="IWI57_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2450268.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3633..3920)
                     /locus_tag="IWI57_002143"
     CDS             complement(3633..3920)
                     /locus_tag="IWI57_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450269.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4091..5443)
                     /locus_tag="IWI57_002144"
     CDS             complement(4091..5443)
                     /locus_tag="IWI57_002144"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBC2450270.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5716..6162)
                     /locus_tag="IWI57_002145"
     CDS             complement(5716..6162)
                     /locus_tag="IWI57_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBC2450271.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6398..7324)
                     /gene="cysK"
                     /locus_tag="IWI57_002146"
     CDS             complement(6398..7324)
                     /gene="cysK"
                     /locus_tag="IWI57_002146"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBC2450272.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7464..8351)
                     /locus_tag="IWI57_002147"
     CDS             complement(7464..8351)
                     /locus_tag="IWI57_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBC2450273.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2288 a         1740 c         1405 g         3002 t
ORIGIN      
        1 agttaccatc ctagctactg agtatgtaca tttacttttt catgcaaaaa agataaacga
       61 aacagctttt ctccataata acaagataat tcatacattt atttgcaatt aaagaaacca
      121 cgtctacagg cgtggtttct tttttagcgc cttttctttt agacgcatcc gatttttccc
      181 atcttgacat attgatagca aaggacatac ctcgcacttg ggatttcttg ccgtgcaatg
      241 atatctacca aaaaaaatca gtgtatgatg tgtctttacc cataattctt gtggtacttt
      301 ccgcatcagt gtttcttcta cttccatgac cgtagcatct agtttgcaga tcctcaggcg
      361 ttttgagaca cgttccacgt gggtatctac tgcgattgct ggtatcccga atgcatcccc
      421 caatacgaca tttgctgtct ttctgcctac accaggaaga gacatcaatt cttctcttga
      481 cgttggcact tgcccatcga aacgttcaat cagctgctgt gcacacgctt tgatgttttt
      541 tgctttattt cgatacaaac caatcgtttt tattttaaga atgatttcat cgattgaagc
      601 ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg ctttattcac
      661 tgatacatct gtcgcctggg cacttaagat cactgcaatc aataactcaa atggattatt
      721 gtgcttgagt tctccatgtg cttcgggaaa cattccatac atcgtttcta gagcttccat
      781 tgttttttgt ttgcttaaca tattccgcta ctcctccaac caatttttca gtgagacttt
      841 cggcaattct tcttgttgag ctgactgttc tacttctctt tgaagaagct gctgttttct
      901 tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga ttttttcttt cccacgatag
      961 caaaatgcgg tcaacgtaat taaaactata agcctgattt aatacggctt cccgcagtgc
     1021 tagcttcaaa atttccggct gataatggtc ttcttctagc cattgtccga tccgctggaa
     1081 ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat aattgataca ctgactggat
     1141 ttctttttct tgtgtttttt cttcgtattt tttttcttgt gtttttaaat actcacctaa
     1201 tagatcatag atggggtaaa gattatatcg atctgctttt ttaccgttga caatcgtcgt
     1261 ttctatcttg ataaatccat ttgttacgag acgatccagt atttgaaaaa tctgatcttg
     1321 tttgattccc atatccatag caatcatctg caaatcaggg aaagtatctc cttcaagctg
     1381 tgccatatgc aactgtagaa caaataaaaa ttcttcagat tgtagaccga tcctatgata
     1441 atttttaagt aaaagattag atatgactgt ttgtccagcg cttaagtaat tttctaaatt
     1501 caacatgatt tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg gaagcaagaa
     1561 caggtttctt ttctatttac ttcactgatc ggtcagattt acaatgtcgt ctacttgatt
     1621 tttgtttgtt catgtttttg atgcgtatct tcacttttcg gaataaataa attaagtaga
     1681 tttcgctgtt ccttagaatg ttgacaagga aacaaaatta ctatacacta tttttgtttc
     1741 caagttaaca ttattgcata aaggagtttt tacatgtaca gtttcacaaa taaacattct
     1801 aaagtttttg gatacgtctc tgtcttattc actggattag gatttactat cgtcagtcct
     1861 gtacttccgt ttttaacgct gccttattct cactccatac atcagcaagc tttctatatc
     1921 actttgctca tgtctgttta tgctctagct gccttcttat ctgcccccat tctcggtagc
     1981 ttaagtgatc attttgggcg tcatccgatc cttattatca gtttattagg atcaagtatc
     2041 ggatatttgc tttttggttt aggaaattcg atatggatgt tgttccttgg tcgtatcatt
     2101 gaaggattga cggcaggaga aatctccacc ttatatgctt actttgccga catcacggaa
     2161 cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt tagtcggtat aggaacaacg
     2221 ctgggtccta ttataggggg tctattagct gagttgggga attctgttcc tatatttatt
     2281 ggagcactct ttactttctt aaatgccgtt tatggctaca catttatgct agaaagcctt
     2341 ccaataaaaa aacgttcagt agatttaagt ttctcccatg taagaccttt tcaccaatta
     2401 caacaattat tcaagatttc atccgttatt cctttattga cagcaggttt tgctgtttgg
     2461 ttggcagctg gctctcttca atctatcttt tctcaatttt cgattgacgc ctttcagtgg
     2521 aaggctgggt tggttggttt aagcttctct ttgattggaa tcttggatac tgtctcgcaa
     2581 ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac aagaataggg
     2641 atgttcagtg aaatcctcgc ctatctattc atcacattat ctggaattct tctactgccg
     2701 attttgtttt tatttgggat tatctgttat ggtttcggtg attctatttt tactcctgta
     2761 ttcaatggcc aactatccaa ttctgtctca gataaccagc aaggtcttgt aatgggcggt
     2821 acccaaagta tacaatctct atcgcgcgtc atcggcccgc taatcgcagg acagctttat
     2881 gctgcagcac catttcttcc gacggtattc ggatttttgc ttatgatttt tgcctattat
     2941 cactacaata aaaaatagta gcttcatttg ctacttcgta taaattctct gtatcgaccg
     3001 ataaaacgaa atactgtgtc aaattcttct tttgacatat tttcaaatac agtagcatct
     3061 cgctgtaccc atttttgatg gagttgctcg tgtctattcc ttattttttg tccgattggc
     3121 gtcaattgaa aatagatttc ttttttattc tcttcgcttt gataagtgtt tatgatccct
     3181 ctagctatca gtttttttgt tagcttactg atggctcctc tagtcatgta actagcattc
     3241 gctaattttg tcacgttagg atcagggatt ttcccgatat gttcaatcac ttctagttca
     3301 tttagagtaa atcctgcaaa ttctttttcc atttctggtt tattacgatg aataatctca
     3361 ttagctaaat ccagatattc ttctattagc agttgtcttt tatccatttc tacaccttct
     3421 ttttctttca ttatattgtt tttttgtttc ctattcaaca aagataaaaa tattttgtag
     3481 gaaaacccaa tctgaaacca agaaaaaaag gggttgacaa agcttgtcat aacccctttt
     3541 tctgattaaa agatagtaaa gagacagtca tcggtaattg tgatgatttt ttgtcatcac
     3601 ttaaggatga gcggcctttt caaaaactgt ctttaaagat catcatcagt ttctagcaca
     3661 tatggctgac cttcatatgt gtactctgta ttcaatgtgc cgtcattttc atctgagtag
     3721 atgcgtctac tgaaaggaaa acgatgagaa acattatctt tggcgtcatt tgctggcatc
     3781 gtgtaatatt cctcttgcgt ttcattcaac tgagagagta cttgatctgc caactcatca
     3841 atattgatcg ttaatttctt cgaagcactt ttataatctt ctaaaaatgc atcttgttgg
     3901 acaaaatatt ctttattcat cttctttctc acctttcttt tcaaaatcaa ataaagctgc
     3961 ttgttctttt atgtaacagc tttatctttg ctgcttattg tgacaaaaaa ttcgaagaaa
     4021 gacaactaat tgtacttttc acaaaaaaag acagagggtc gtgacaaaat tgtgtcacaa
     4081 ccctttttct ttattgtgca gtttcgattt cgcctagcgc tgcttgtgca agtaggttca
     4141 agtaattcca tgggcggtca aaatgtggtt ggaagaagaa atctgaaagt gcgagatctt
     4201 ctacagtcat tttattttga acagctaaag acatggtatt tgctgattgt gtaatgtcgt
     4261 atttggacat gaactgagca cctacgatac gattcgttcc tttttcatag actagctcca
     4321 tcattacttt ttcagtagtt ggcatgaact ctggacgata attatcttca aatacagtcg
     4381 cttggacttc tagatcgttc aaagttgcgc tttctttggt cacacctgtc gaaccgatcg
     4441 tccagccaaa taggtaaagt cctgaagttc cttgagtacc acgatacgcc attttatgat
     4501 ctgtcaagtt atttcccaca agcaaacctt gacgaacggc atttgttgcc aaaggaatat
     4561 agttttgcgt gccacttgga ttgtagttga ctactgcaga gtctccagct gcaaaaatgt
     4621 ctggattact tgtctgcatg tattcattca ctttgattgc cccgtttggc agtgtatcta
     4681 cttttccttc gatcagtttt gtatttggac ggaagcccac acataaaatg accatatctg
     4741 tatcaaatgt atctgttggt gtagctactt gtttcacttt tccttcttca tctgtgataa
     4801 attcagtaac attttcgcct agcgcaagag taacaccgcg gtctgtcaat tctttttcta
     4861 agatatctgt gaatggttta tctagatatt tattcaagat gcgatctaat ccatcaatca
     4921 atgtcacttg ttttccagat tcgacaaaag cttctactaa ttcaataccg atatatccac
     4981 caccaacaat tactacacgt tttgcttctt tcgcttgtgc aataatttca tttgcttgat
     5041 tgtagttttt gcataggagt acatttttcg catcgattcc actaatcggt gggataatag
     5101 gccatgaacc agttgtcatc actaatttat cataagaaac cgtttctgtt tctcctgttt
     5161 tcagatcttt tgccgttact gttttatttt ctgtatcgac gtctgttaca tcgtgttcca
     5221 ttttaactgt tgctcctaat gaagataatt cttcaggatt tgagtagaac aagcctgctg
     5281 ggtcctttac tactccgccg acataaagtg cgataccgca agataagaaa gaaatattgt
     5341 catttctttc atatactgtc acttctgctt ctggatgatt ttttaaaata ctttttacag
     5401 ctgcagtacc tgcatgagta cagccaatta ctactacttt catttatggt ccctccgatt
     5461 aacattttgt ccttcgacaa aactactata acacaaataa aacggataca acagtttaat
     5521 gcttgtgaat tcacaaaggt ttgtttgtag tttcattcct tttcaagcat aagaacccaa
     5581 tatctacaag attcttattt taataatatt cacttttata tctaagattg tgaataaaag
     5641 acaaaaaaat aaaaaagcta gcgttttttt tacacagcta gcttttttta ggtataacac
     5701 ttgtagaaac tgtttttaca tttgcgcctt ttttgctgac tctttacaat ccggacagag
     5761 tccatacaat tccaaccgat gttcattaat ctcaaaatca gtcagtctgc ttgcagccat
     5821 ttctatatct tcaagccctg ggtaatgaaa atcaacaatc ttaccgcagt tttcacaaat
     5881 tacatgataa tgacgctttg aactgaaatc aaatcggcta gaagcatcgc cgtacttcat
     5941 ttcttgaaca aatccaatcg aagtaaataa gcgaaggtta ttgtaaacag tcgcaacact
     6001 catatttgga aaccgatgtt caagtgcttt ataaatctca tcagccgttg gatgggaatg
     6061 atgttcaatc aaatattcta aaaccgcgta ccgttgaggt gtgattcgaa tgttctcttt
     6121 ttttaacttt tcgataacct cttcgactaa cttattttcc atgtaaacac cccacgttgt
     6181 tattctcgtc ttatcatact atttttacat tttaaaatcc acttaacatg aaaagaaaat
     6241 tcaattttat tgtattttaa tgaaaaaaga cgtaaaacag tcgcgttcac tattctacgt
     6301 ctctattcaa ataatttttc tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt
     6361 tttttacata aacctatctg gagaaaaacc aagtgcctta tatctcttgg taaagggctg
     6421 tagataaata acgttcacca ttgtcgggaa ccacagctaa taccttcttg ccttttccta
     6481 attcttttgc gacttttagc gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa
     6541 tcccttcttt tcgcccaact tctctagctg tttccatcgc ttcgtctcca ctaatagaaa
     6601 ggactttatc atacacagaa gtatccagtg ttttaggaac aaaacctgta ccgattcctt
     6661 ggattttgtg aggtcccgct tctttccctt ctaaaatagc agattctgct ggttctaccc
     6721 cgattaattc gattttcgga tacacacgtt ttaattctcg gccagcgcca gtgatcgttc
     6781 ctcctgtacc gatacctgca acaaacgcgt ccaagccatt tactccaaat gcttgatgta
     6841 tctcaggacc agtcgtcttc tcatgcacaa gaggatttgc ttcattttca aattgtaatg
     6901 gtaaaaagta gccgttttct tttgccaaac gttctgcttc tctgattgat ccagaaatac
     6961 catccgcacc aggagtcaaa atcaactctg cgccgtaccc tttcatcaat aaacgtcttt
     7021 cgatactcat cgtctcaggc atcacgatga ttactttata tccttttgca actcctacca
     7081 tcgacaatcc gattcctgta tttccagaag taggttcaat gatagtatca cctggtttca
     7141 gtagaccatc gtgttctgct ttttctatca tacttaaagc aatacgatct tttacacttc
     7201 ctcctggatt aaaaaactca agctttacaa aaacatctgc tgaatcttct ggtacgattt
     7261 tatttaattt gacgattggc gttttgccaa tcaattcggt gactgagtta tagatagctg
     7321 tcatcttcaa ttcctccttg tctttgctct tcttacttat cataatacaa tctgttcatt
     7381 tatggcgagt gattcgtctt tgactagcaa aaaaaagaca aagatatacc atatatggta
     7441 atttctttgc ctttttatct aaatcaaacg tttagttctt gaccaactaa taaagaataa
     7501 ctggacatag acatgccatt tttcgccatt agatcatcaa cagacatgcc aaatttttgt
     7561 gcaatatccc atagcgtatc acctgacacg actgtatatg tctgaccatt atttgtattt
     7621 acatctgttg ttgaagatga tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt
     7681 gtggttgttg tagccgaacc tgttgatggc gtatcaaaac gggttaaatt ataagtatta
     7741 atcaatgagt tcaatttggc cccataattt ggatcggtag cataacgacc tgtcaagtac
     7801 gctgttgcat cataaaaact tgttgtattg cttttccaca cgccagcata atgataatcg
     7861 cccgtggaaa aactggttga acgaagaaca ttcgcatggt cttggaagga ttcccaataa
     7921 gaagaatatt ttcggaatgg ctcattcatc gttacccatt gtccgtttac atattctttg
     7981 gttgccatat aaacgacctg tccgttatag cttcctttca caccgaatag attataattt
     8041 ggcgcactag ctaatccgga agtgccatat gcactttcca acaacgcttg agcgatcatg
     8101 actgaagcat acaagtcatt acttgcagca acttctgacg cagaccagcc gatttgatcg
     8161 ataaacgctt gaggatcagc gactgtttga gatccttgtt catctgcatg aacatgttgc
     8221 ggtaccgctg taaccatcat cggcccaact aaaagactcg ttccaattaa aggagcaatc
     8281 gcttttttat tgatcaccat ttttctttta ggtctatggt gccgggcact gcgggtcttc
     8341 agctcttgca ttcaaattcc tcctcaaaat aaaaaaactt aataaaaagt tctcttctta
     8401 aaacaaactt aattaattaa attatacttt taatt
//
LOCUS       DADXTG010000109         8180 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.110, whole genome shotgun sequence.
ACCESSION   DADXTG010000109 DADXTG010000000
VERSION     DADXTG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.110"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(264..971)
                     /locus_tag="IWI57_002148"
     CDS             complement(264..971)
                     /locus_tag="IWI57_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450274.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1036..1872)
                     /locus_tag="IWI57_002149"
     CDS             complement(1036..1872)
                     /locus_tag="IWI57_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2450275.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1890..3299)
                     /locus_tag="IWI57_002150"
     CDS             complement(1890..3299)
                     /locus_tag="IWI57_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2450276.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3313..5136)
                     /locus_tag="IWI57_002151"
     CDS             complement(3313..5136)
                     /locus_tag="IWI57_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2450277.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5416..5643)
                     /locus_tag="IWI57_002152"
     CDS             complement(5416..5643)
                     /locus_tag="IWI57_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450278.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(5979..6305)
                     /locus_tag="IWI57_002153"
     CDS             complement(5979..6305)
                     /locus_tag="IWI57_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450279.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6351..6713)
                     /locus_tag="IWI57_002154"
     CDS             complement(6351..6713)
                     /locus_tag="IWI57_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBC2450280.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6750..6965)
                     /locus_tag="IWI57_002155"
     CDS             complement(6750..6965)
                     /locus_tag="IWI57_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450281.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7092..7427
                     /locus_tag="IWI57_002156"
     CDS             7092..7427
                     /locus_tag="IWI57_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450282.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7503..>8180)
                     /locus_tag="IWI57_002157"
     CDS             complement(7503..>8180)
                     /locus_tag="IWI57_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450283.1"
                     /translation="DEDVPLKMLEYLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQ
                     KLNEDILPLLEKIHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEAH
                     EVLQGLSWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASK
                     DIKKLYNVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKR
                     KDPIVLN"
BASE COUNT         2506 a         1740 c         1270 g         2664 t
ORIGIN      
        1 tctaaaacac ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa
       61 taaatatagt cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac
      121 tggagatgta taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg
      181 aagaatcatt attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc
      241 ttaaatacca cagaataatc tttttaccca ttttcaagta accgaagtgg aatgtcttta
      301 ccaacttgct ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa
      361 gattgagcat ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt
      421 acaagcttcc aagtaggtgg tatagctgtg tatgtttcga tgaatgaatc aattctttca
      481 tctatcgtct tcccagtatc tacttctgtc tcattactct tagatgaatc gattctttca
      541 tccatcgtct gctccgtaac tgcttctgtc tcattattct caccggaaac tctcttcaca
      601 cccaaactta tatattgtcc atctttagtg tgcatagtta aaattcccac gcttttacta
      661 ataaattcac tataaccagt tctttcaata gagacttcaa tgtatttcac actatcatac
      721 tcaaaagaaa aatagacatg taaaagcatt ttgttgttta catctacata ataatccttt
      781 gactctactt gagaccttcc tatacggaat acagctttac tgtctgatat ctctatacca
      841 gcatccttat tattaatctg ccaatctcca agtagctctt ctggcaagct agtagtttct
      901 tgtgaacact ttaccacttc actggtagag gtcgaaacat tcacctccgc actgttagaa
      961 gaattttcca aggaatcagg agccttagca caaccaacaa gtctttatat attgtgcagc
     1021 cgatattttc ttcattcagt acacccttga aatatgcagt gttagataaa gcagttcgtc
     1081 ctgatttgct gaggcaccca tctcttcttg aacgtactcc ttgattttca aggccgcccg
     1141 atactctttc ggaaaacgct gcttcactga agccagcaaa aaatcatcct tctccggcaa
     1201 ctcttccttc ttaatcattc gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta
     1261 aagcatcgaa tctttatcaa cctttttttc gggaaacaga tatcctagca tagccaagat
     1321 atcctttagt gctcttaccg agagcatggt ttcttccata tttgcttgtt cactttgggc
     1381 atttacaatg tgaaacgcaa tgtttccagc ctcctcttct ggcaactgaa cattcatttt
     1441 tttgttgatc aattggatag catagcagcc gatctcaaat tcttttgggt aatacttttg
     1501 aatttcaatc agcaggcgat tttgaaaaac aatgcctttt tgactgcgtt caatggcatg
     1561 cttcaagtga tcaaccaagg taaaaaatag acttttattc agcttaaagt caaacttggc
     1621 gttggcataa tcgataattc gcttgacgca aataattaga tcatccggag cagcctcaaa
     1681 cagtttaatg taatcctttt ttagagcatc agaaagtgaa tctaatttaa atactttttg
     1741 gattttgtca gccggaacca aatcgccttt ctttagttga aatccaatcc ctttccccat
     1801 caaaattact tctttgcctt gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt
     1861 aattactctc attagattca ccgttctctc taagctaagc catttcttgc gattactttt
     1921 tgataccaaa agaagctttc tttttttatt cgttttaatt ctttaagatt ctcatcggtt
     1981 cggtcaacat atataaaacc ataacgctta ttgaagcctt gatgggtact aacaacatca
     2041 atcgctgacc aaggtaaata accaaaaaca tctacaccat ctgttaaagc tgcttgaata
     2101 gccagaatat gtttggctag ataatcaatt cgatcctggt cattaattcg tctatcttcc
     2161 tcaactatgt cgggtgcccc atagccattt tcagtaatta aaataggtaa atcatagcga
     2221 tcgtacaatt tcctcaatgt taatcgtagt ccagtagggt caactaccca gccatatggc
     2281 gttttatcta cataaggatt ttcagctgcc cggtagacac cttcttctcc caacatgatc
     2341 tgttgatccc ccgctctagc ggaaacatct gatccgtcat ttttgctggc agcgatggtg
     2401 gcagttgaat aataattaat cgcaataaaa tcgggtttac cggaggataa aattgcctga
     2461 tcctctgcct caattttagg atataatcca cgatcattca aatagttttc aaacaaataa
     2521 ttgtattttc cccgaactgc tacatctaag aaagaccagc ctctcaatgt ttcccaatta
     2581 tgggctgcaa ttgcatcttc tggactggag gtagcttgat acatagccgt taaattgatt
     2641 gctggaccga tttttccttt aagattcaac tgatgataca agtgaattac tctagcttga
     2701 gctaataaaa catggtgatt aatttgaaag agttcttttt ttgaaggcag ctctccactt
     2761 ggtggcaaac caatcgctcc aggatgaagg atcatcgtgt tttgctcatt aattgtcagc
     2821 caatagttca ccttgtcacc atactcagta agcaatacct gtacatagtc cacaaaataa
     2881 tcgatagttg ctcgatttaa ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa
     2941 tggtacatgg tgacaatcgg ttcgattccc tgattcaggc attcatcaat tacttgatga
     3001 taaaaagaaa ttcccttttg attggtttta ttattttcaa tatccataat tcgcgtccaa
     3061 gcgatggaaa agcgaaaagc ttttaagcct aattctttaa acagctgtat atcttcttta
     3121 tagtgatggt aaaaatccga ggcaacttca aaattagcta ccccttctgt atgctgatac
     3181 ttgtcaataa tagacggttg tttcccatcc tcctgatagg caccctctgc ctggtaggca
     3241 ctaatggagg caccccataa aaacttctct ggaaatgctg aaagctgttt gtattgcaca
     3301 aaaatcctct ccttaactaa tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc
     3361 ttcactaatt cctgcgtaat tttcactgtt ggtcactacc acaatcactg tatcatcgta
     3421 gccagcgttt ctaatggcag cgaaatcggc gttgattaat aattgtcctg ctgacacctg
     3481 atctcctgct tttacaaata cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc
     3541 cacatggatc aataattcaa taccttcttt agaacgtaga ccaatcgcat gtttagattc
     3601 taaaatagta atgacttctc cctcaacagg tgaataaatg ctgttttctt ctggatgaat
     3661 ggctacgccc tttcccaata gtcccttaga aaagacagca tcgttcactt cagaaatagg
     3721 acacacggtt ccttttaccg gattttcaac aatgactgct tcggctattg ccgtttgctc
     3781 tgattcatca gaagtatcgt caaatccaat tagataagca atgataaagg ttgcaacaaa
     3841 ggcgattaca cacgtaataa ttgcgtgaac aatattcatt ggattttctc caataaaggc
     3901 tggtaaggct gctaagccag gggaaacaaa ggcatatctg actaaaccgc tcaagccagc
     3961 atacaaacca gcaattgcac caccaatcat ggaagcaact aatggttttt tcagcttcaa
     4021 ggttactcca tagagtgaag gctcagtaat tcccattaaa gcagtgatcc cagtggacac
     4081 agctagctgc ttcaactctt tgtttttagt tttcgcggct acagcaaagg tagcacctga
     4141 ttgagcaata ttagaagcca gcattcctgg cccattgatc atttcagaac ccgtactgct
     4201 caattgcata gtagcgattg gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg
     4261 ctgcaaacca cccatcaaaa atggaattaa ccaagctgct ttgccgttaa tccattcagc
     4321 ccccattgct ataagatcgt tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa
     4381 aggccccata attaaaaata ccagcatcgg cttagtaaag aatttaataa acgaaggcga
     4441 aattcgatcc gcaaagggct ctacatagga catgatccaa acagtaaaga taatcggtaa
     4501 aactgatccc gcataatctg cagctaatac cggaattcct agaaaagaaa cggcatcgcc
     4561 agtagaaaaa agctgtgaaa tatttggatg aagcaaaatg ccagcaacag taatcgccaa
     4621 aattgggttg acatcaaact ttatggctgc cccataggct aatagtactg gcataaaata
     4681 aaaggctgca tcagcaatag tattcaaaat gatgtaagtt tgactactgt ctgatagtac
     4741 accagtcaaa gtcaagacgg ccaaaatggc ttttaccatc ccggcacctg ttatagctgg
     4801 aatcatcggt gtaaaggtgg tagaaatgac actaatcaat cttgaaataa tactttcttg
     4861 attgtctgaa gaggggccct ttttaattga agttcccact tccttttgaa tttccttgaa
     4921 ggctgtttgg acttcattgc caatcaccac ttggtattgt ccaccgctat ccaccacact
     4981 aattacacca agtgtttttt ctaaggcttt cgtatcggct ttccttttat ctttgagttc
     5041 aaaacgcagt ctggtggcac aatgggttaa gctgaccaca ttttcttttc ccccaacctg
     5101 ttgcaataca tttcttccta cttctctgta atccattatt ctctctcctt aaagcagtaa
     5161 aaaagaccaa gtcaaaccac gccccaatca acattgaagt gttagtttgt cttggtcttg
     5221 cctgcataat cagtaacatg ccaataacgt tattttgctg taatccaata atagcacatt
     5281 atgatagcgc aatcaacagg gaatttaatt ttttatcttt tcgtttgacc gtatagggta
     5341 atataacagt tagatcacat caactagggt atatctgcgt atacttactg ctaaaataag
     5401 tatatctaat attaactata ccagtaaaac cattcctaca taaccagcgg gaaattccca
     5461 aaatcgtgaa agcatcaaaa atgcatataa cggatcacac aacttcaaca attctgctct
     5521 tgggaaaaat tgatgaattt ggtgagtaaa ggatggaata aacagcattc caacaatata
     5581 ggcaacaata gtgatacttg tttttttggt aaaaatcagt aagcaatagc caatttcaat
     5641 cattccgaga tataaaggta tcaatagaat gatttgggct agtattctta aaaattaact
     5701 agcaaatgtt taaactgatt tatttctaat ctacatgtag taagtatttc aattccacca
     5761 attatcaata aaaataccaa acatatccat aataattttt tctttttcat ttcttttata
     5821 ttgttataac agtgaataac ataaaaattc ccgacaatca ttgtaatatt tttttgtcac
     5881 ctgtattagt atttaattta taggaaacta taaaaactcc ctagtgaatt tacacctttg
     5941 ggagttcctg tttggacggt atgcataata catttaaatc aatttaacat taacacctca
     6001 gcatatggta tctccacata tatacggtct ttctttggat agtactcaat atttatgttg
     6061 atagcacttc cattgtctaa tatagcttta gcccggtatg tgttatctcc attttcatga
     6121 gtaatcgtca catcatcaca tgatcgattt aagttgtact gttcttgtaa aatctgtgtt
     6181 accaatggtt ttgatgactt ctctaatcca gatgtacctg tcatttttga aaaaataata
     6241 cctataatag ccaagacaac aatacctata atgacaaact tcttgttcat atttacttta
     6301 ttcaactttt tatctcctat ctccaaaata atttgttctt atccctctta tcagtacatt
     6361 gacatcccta aaagtacacc aaagattgct ccagctaccc ctgctatcat caaaatcgta
     6421 cccattttct catctttttc tcgatataag tagcccataa taacagcaac agcaccaaat
     6481 acaacaggaa taaatgctaa cgatactacc atagaaatcc aaccaataat caaatacagc
     6541 ttccctgtct cttgtccatg tacgatttgt tccttcattt cattaattgt ctgctcagta
     6601 cttttcttta ctgtgtctgt tccggttgtc tcatctgttt cttgtagtgc tccacaaaac
     6661 tggcaatact tactttcatc tgttatttct ttgccacatt ttctacaata catgtcttct
     6721 cctcctaaaa tcattcaatt gcttattagt taactgttta ctttattaaa tacatcagaa
     6781 aacagctcat tcattggtac acttaatatc tcagaaagca tcttcatttg atagggttta
     6841 ggaatcgttc tccctgtttg ccacgaactt acacattgct gtgtaacatt catttttttt
     6901 gctaactccg tttgagaaag tcctttttct tttctgtatc ggctgagatt acttactttt
     6961 ttcatgattc tcccctcctg ttttattata tacaacaata tgatgtattt caactcataa
     7021 aatataaaaa acttcaaata ccattacaaa tacaacaact tgttgtacta tactgagatg
     7081 ggggtgtatt aatgaaacag aaacaatatg caaaggtcat tagagaaaag cgaaaagctc
     7141 tcgggtggac acaaaaagaa ttagctgaaa aaatcttctc tacacaacag gcagtagcaa
     7201 gatgggaaaa ttctgtaaca gagcctaacc tcgatagctt aacagctctt tctagagccc
     7261 taggaacccc tgtaagccat tttctagaca atgttgtcgt agattatgag gaagagtttt
     7321 tggctctcta tcgttctctt agcactgaag atgcagttcg aacaattgac tatatgaagc
     7381 tattaaaaag gcaagaaaat gaacgcaatc agctattgaa agattgaaaa ataaataacc
     7441 caccgaactt taacggtggg tgtattgaga tgaaataaga ttaagagcta tggataacct
     7501 gtctagttta atacaattgg gtcttttctc ttccttactc tccgtccatc atctcttgtt
     7561 acacttgtaa attgatacag aggaactatc ccagctcctg ataatatcaa ttcttttatt
     7621 gtttcatcta tccctgcccc tttcgctaaa tatattaaaa aactactact tgctttcaca
     7681 ttgtatagtt tcttaatatc cttagaagct cgttcaagag tgaatccttg tttaacaaag
     7741 tagcctctgt tttttattaa agcatttttc acgctgttat ccccaacatt acgtaggcgt
     7801 tcacaaacag tgttaataag ttctttctct gtcattagat atgtcccttg ccacgataag
     7861 ccctgtaaaa cttcatgtgc ttcatctaaa atcaattgtt tttccttgct gagttctgat
     7921 cgggaatttt gaggaaatac tcttttagcc tctttttcgt taaaacatat cttaacccat
     7981 tcataagaaa aatattgata tgtaaaatga attttttcca gaagtggaag gatatcttca
     8041 ttcaattttt gaatcacaga agttgcatta aaaacgtcta atcgatagac attgcttctc
     8101 ttatgattaa agtttcctaa cgctttctct tcctttaacc tatttaggta ttcgagcatc
     8161 ttcaaaggaa catcttcatc
//
LOCUS       DADXTG010000110         8123 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.111, whole genome shotgun sequence.
ACCESSION   DADXTG010000110 DADXTG010000000
VERSION     DADXTG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.111"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..525)
                     /locus_tag="IWI57_002158"
     CDS             complement(<1..525)
                     /locus_tag="IWI57_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBC2450284.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLS"
     gene            complement(522..941)
                     /locus_tag="IWI57_002159"
     CDS             complement(522..941)
                     /locus_tag="IWI57_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBC2450285.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1011..1151)
                     /locus_tag="IWI57_002160"
     CDS             complement(1011..1151)
                     /locus_tag="IWI57_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBC2450286.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1144..1491)
                     /locus_tag="IWI57_002161"
     CDS             complement(1144..1491)
                     /locus_tag="IWI57_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450287.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            complement(1505..1999)
                     /locus_tag="IWI57_002162"
     CDS             complement(1505..1999)
                     /locus_tag="IWI57_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450288.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1999..2448)
                     /locus_tag="IWI57_002163"
     CDS             complement(1999..2448)
                     /locus_tag="IWI57_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450289.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(2452..3072)
                     /locus_tag="IWI57_002164"
     CDS             complement(2452..3072)
                     /locus_tag="IWI57_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450290.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3072..3980)
                     /locus_tag="IWI57_002165"
     CDS             complement(3072..3980)
                     /locus_tag="IWI57_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBC2450291.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3993..6755)
                     /locus_tag="IWI57_002166"
     CDS             complement(3993..6755)
                     /locus_tag="IWI57_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2450292.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            complement(6752..7492)
                     /locus_tag="IWI57_002167"
     CDS             complement(6752..7492)
                     /locus_tag="IWI57_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2450293.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(7482..>8123)
                     /locus_tag="IWI57_002168"
     CDS             complement(7482..>8123)
                     /locus_tag="IWI57_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBC2450294.1"
                     /translation="KNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRV
                     KEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSP
                     SRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQN
                     ISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT         2346 a         1718 c         1143 g         2916 t
ORIGIN      
        1 tgataagtat ccatacggat cagtgtgatc gccccaaatg ttttgagtta cccataaatg
       61 tgatttgatt cctggttgat tatacggtgt gtctaatgta agtggaatac cgtatttatt
      121 agcggaatct ctcgctaatt caacatatgc cttgtagttc ttttcaaacg ttactttatc
      181 gtgcgtatgt tgcagttcga tttgcacagg actatttgca ttagcatatg aaccagcgcc
      241 atattgtaca taaccaggtt gtccaacttg gtaaacgatc ccaccatcgc caacaatata
      301 cgctgtatag gcattgtacc aagaacgttt catgtactgc gcttcatttc gtcctgtcgc
      361 agtttcgttt gctgtttcat gcaataggat gtattggtta tttgctactt gagaactacc
      421 ttcgtttgcg cccaaattga attcattgtt gatcgagtaa gcaaaggcgt ttattggcaa
      481 taaaaaaaga gctataatag ctcctacaaa gattttcttt ttcatttggt tcctcctcgg
      541 tctttatttt cagtatcatt tcgtatttgt tggaatacct cttttaactt atctggcaaa
      601 ggaaggaata ccgaaatgtt ttctaaaaaa ctaatccctt catttgcaac atagaacata
      661 ataactattt ctcttagagg tatagaattc ccaacgattt tctgtacgtc aaccgcaacg
      721 gcaatcacaa cgaagattaa gactttcttc aacaatcctt cgaaacctat ctcactactt
      781 atttctttca atttccatgc cttagcaagt ccagttaaga agtccacacc cacaagaaaa
      841 attaaaacat gtagtatatt gtccatccct cccaataaac caacaattgt cccgcctatc
      901 acccctgtca ttactgagag tgtatttaaa tatttttcca atatctattt ctcctttatg
      961 caaagaagcg cactcgtttg agtacgcttt ttatttaact ttgtgcttaa ttaaatttca
     1021 tttccagtaa tctgcttata ttggtctttt gttaaacagt tcatgtttac atagtcaagt
     1081 aaatcttggt ccgtataaca tccccaatca taaaattgtt ttatattttc aaagcccgga
     1141 aaactattca ttacttttcc ctccattctt aatacttgcg acttccatcg tcaattgagc
     1201 taacatttgt tgcatttttt cttgttcctg taccgatatc gccaattgtt ttgtcagtag
     1261 agcatttaat tgcttcaact gatcgacatc ttcaatatcg tcaaccgctg tgccactctt
     1321 ctcttcttca gtagctaact caacccactg ttttttatca aaatcaaatc ttggtctcca
     1381 gttaggaact ggtggtttaa tctctgtaca gttttcaggg atattttctt gattgttcaa
     1441 aatgatttgc tcgaatccgt aaggcttgat tgatttgtaa actactttca ttagatttcc
     1501 tcccttaaat tgaataagtg ataacaaatg aataagctga tccataactt gaatttcttc
     1561 tccatttaat ggctccgtct gcgccaatag atagttgagc actgttcaaa gtagaacggt
     1621 ctattgaccc aaccagttgt tcaaaactaa ttggtgtccg atagccttct ggaacagtta
     1681 aaataaccga atcattcccg ccactacttt ttccgtttaa agccacaaaa tatatagaaa
     1741 ctgtttttcc ttcacgataa agctttgctg tccctgtatt accgttagtg acaaccagat
     1801 ttaccacttc atatctgtta tcatcagtcg tcaaaacacg tttattattg attgttacag
     1861 aacctgaaaa attcttatgt cctgcaatgg cttggtctcc atctgtctgc accaatgctt
     1921 ctttaccatc aatagctttt acatgcgttt ttaagtattt agcaactccg tcttctttta
     1981 attgaacaat atcagccatt aaaccgtccc cactttctca aatgtaatat tggctaatcc
     2041 atcaagtttc actttatcgg ttgctgacat taaacctgct gtcgtagttg tagcattacc
     2101 tggatttttc tgtgctccag ctgcaattcc atccaactta gttttatctg tggacgacat
     2161 caacccattt gctgtagttg tagctacagc tgtagttgtg gcatttattc caggatcacc
     2221 tttatctccc tttggtaaaa caaaattaaa tctagctgca gatgatgttc ctacattcgt
     2281 aacagaagcg gttgaaccac tagaaacggt tcctatggta attgttgctg cttggccagg
     2341 atcgccttta tctcccttca ccgttgttgg tttgccttct atagcattcc aatgagtttg
     2401 tggataaacc tgtacattgc tttgttttat ttttacgata tctgtcattc tctatacctc
     2461 cccaattttt tcaaaagtaa aatttggaat tctttcgttt gtgtaatttt ctgcttgatt
     2521 tacagcttct tggaattttt gatctacata tgactgattg acaccaccag tcccactacc
     2581 acctgtagaa ctaattgttc catcttcatc aatagtgatg ttttctccag cagtataatt
     2641 tttcagtgaa tcgagttttt cttttaactc cagcgaaaaa ttgacatcag attgtctgac
     2701 tgcagagact ttccctgtct catcaatcgt caataattca ccaactttta tgccacccaa
     2761 acggtcgata gtggcaactg gcaacacgta gtcaccttct ccactgttta aaattttttg
     2821 atacatttct gccgtgataa tgccgtcatc ttcttcactt gcatagggta attctgtgag
     2881 aacattctct aatcccaaat cttctttagt aatgacgacg gctccaattt ttccattaac
     2941 agataaaact gttgcttctc ctgctattat ttcttctaac ccaagaactg cggaagcatg
     3001 tgtgattgga aagaattggc gttgtacacc tgttttttca tccgtttcca tcattcgttt
     3061 agttttagcc attagatcac cccgactttc tcaagagtga acacattttg ctttggatca
     3121 tcaacggttg cgatgatcaa ggctccttct tcaatcggga attctacagt tccaactttt
     3181 tcgacttcgt gattatctga aaagagatcg tcttgaagaa tatcagtcac ttcaatttca
     3241 ccgtattcaa tcgtgaaaag tgctgctttc attttttgat acaaataatt tagatcggat
     3301 aggaaacgtt cagaaatcga ttcatggcgt acaccttgaa gatctacacg tgcatccatt
     3361 aattcggcta acattactcc acctggatcg atagacttca aaatatcctt gattgattca
     3421 aaccatgaca aataatctga ttcttgtcct tctctccaat cttggaaact attctcctgt
     3481 tcttctctcc agcgatcaaa ttcttctttc ctttcattca tccagtccgt aaaatcgcct
     3541 ttattttcat taataaaatc tgtcatatca gcaattaaat cttcaattga ttgccaataa
     3601 gaacccattt ctccttctgt ttttgaaaca gcattgatga caaaatagga gaagtcttga
     3661 gttgttccaa tcaagtcttc tcctttaaat ataatgaaat tggctgtttg tcgatgcaaa
     3721 cactgcatgg aatatttatc aaaaatatat ttgattttcc cttttttagc atccacaatt
     3781 cttgtttcta attggactgg atattttccg ccaacaactg attcaaaata taccttacat
     3841 tctgataaat catatggaag accattttcg acaattgtag cttccataac ttcagtgttt
     3901 ttattgcctt gtcgaacttg aatcatcccc acgtaattat agggttctgt tgtacttaat
     3961 atgacattcc actttgccat taaatcacct ccctattttg gtggtatgac aatggaagaa
     4021 attgcacttg cactataata ctggcgatct aactttccac aaatcatacc taactctgtg
     4081 ttttgttcat aagtttgcat acgaccatta gctaagcctc ggataacgcc agtatgtcca
     4141 taagttccgt ctgcaaacca tgaacccact tgtccacctc ttgcccaatt aataattgca
     4201 ccaactacta attgatcata tctagggttt tgaatcactt tccaaccaac agcagaccaa
     4261 tcataggcaa taccaatatc agatgctgca gaagtatttc ctatcacatg agttaaacca
     4321 tattttgttc cagcacccat gccacaacca cccagatatc ctgaatattc tgcagacaag
     4381 ccataacact gaccattgcc aattcgttgt ccaatcaaag actctaaatg ttttagtcct
     4441 gcttctccag tagctcctcc tggttttaaa tctttaaatt tgttatacca gttaactgca
     4501 taatcttgac gttcaggatg tgtcgctgcc ggacgttcat agtttcgttc aaaagcatat
     4561 gctgcttgtc ttggatcagt acaggcctta aatccatcaa ccgtcgtagg ttgtactacg
     4621 cccatccatt gtccatttgt aaatgtccaa ataagcaatc gaacttgtgc atctaagctc
     4681 gttattggtt ctttaatacc tgcagcatta aataagtttt gaacataaac ttttccatcc
     4741 catgttgctg gaccaacaag aggatatgaa gaaccatccc actgaactaa tccgtatgct
     4801 ggtccaccta tttgaacagt atcaggatca aaagttcctc ctgtttcttg ttgaatgttc
     4861 cccaatattc cacatgcaga ttgtttcgta aatccgttat tacacaaaat atcgtaaatt
     4921 ctccaagctc tcttttctgc atctgttttt aattcactag gatacccacc agtagattct
     4981 ccacctcccg atggaccacc accttgtcca ggaattattt cttttccttt aactgtaagt
     5041 tttccttgta catctaagtc tccaaaataa attgctttac catttcctaa taaaactaat
     5101 ccttttccta ctttaggaga aatcaaaata tatttgccgt ctccatttgt acgaataact
     5161 aaagaattat cttcaatagg ggttggagta gaagccccag gaaaaggatt accagcagaa
     5221 tcagttgttc caatcgttcc aattgaaccc ttagaattcc aaaattccat tcctttttta
     5281 gttaactcca ttattttctt tttgttgttc caaatttgga gtagtccatt aactaatttc
     5341 aatacatctc cagtcttgtt aaaagaattt tgaaagatat ctgctttgat taatccagtt
     5401 tgtataaaat tggcattaaa tatactatct aatgtccaag ctgaagtaaa aggcccccgc
     5461 catcctttct tagaaaaagc aatcccattt ttattcattc ttagtacttc tcttgccttt
     5521 tctaaatctg gattatccat aataaagatg ttggaaggtt tttcttttgg ccataaactt
     5581 acatatcctc ctgcactgcc ttgacctgta atcattgaag aaacataatc attaaaatca
     5641 ctcatataat tattcgttgc ataatctttt aatttatcct gaatagttac agcttgttgc
     5701 tgataaaaag caacttgaat gtctcctgct tctaatttca aagttttttc agataaagaa
     5761 tcgtattgta gtccactaac ctttgattca agataaatat tatatttttt atgatatatt
     5821 ttaaatgtat caaacagacc ataatttcta atcttcgcaa attctttcgc ttcttcacta
     5881 tcagtcaact tatcaaattc aaccgtaata gaaactttag gcttatcaca gccaggattg
     5941 atcgttttaa agtaattttt agctatttta tttaagctct taatgtcttt tactccttgt
     6001 tcttctgtaa actgaacatg ctctgtatac acatcaggat agttattaat ataggcacta
     6061 tctactggcg aaccataaat tcgattagtc gtacctacct cactttgagg atcagcatat
     6121 ggaataagtc ttgtttttat acctgtccaa tctaatttga cctttaaacc agacatatct
     6181 tttccatacc gaatcgttcc aacattattg cgacctcttc gctttaacaa agaaagctta
     6241 aatggttcac gtttaatttc tccaccccaa tattgaagca atgatccttg ttcaccagca
     6301 atacaattta aaacatttct cgcttcaaag attgtgctag aaacggccgt aatatcagaa
     6361 taaagtcgta tgtcagattt tttatccatt ttggtttcaa taattgatat cgcttcttga
     6421 ccagttttag agtctacttc cgcaagagtg actacccgtc ttccaagtcg atttgtacga
     6481 ctctgagcat aaatagttac tgtatttaaa aaagtatcta tatctttatc atcaataaaa
     6541 aagatatgat actcttcttg atcgtttggc tttgctttta tttgatagtc attttcaaaa
     6601 tattcatcaa atctagttcc taaaggataa tccaactcta attcatattt tccattagct
     6661 acttcatata tttcacatct tgtacaatcc tttaaaattc ctaagccatt cgttgagaaa
     6721 tctgtttcag taggactata tattcttggt ttcatacttt tctccaccac ctaggcatta
     6781 tttcaaatga atgaatattt ttcgtccatt taatttcatt ttttccagga tataaaaatg
     6841 gaaaatctaa aaataaagtg acttgatcct gatgttctaa attaccatct aattttcggt
     6901 aagcctcttc taatttagaa tctataaata attctctgtc caacgatttc aaatcatatt
     6961 tgtccttatt aatataaaaa gaagcatctc cagaaccact caatttaata agcggttttg
     7021 aagaatactt ctctgggtta taaatttcaa aagtttctgt ttgtcgaata gtaaatctac
     7081 cattgtaatt ttccttaaat ggtcgaatgc tcactgtaaa ttcaaaaggg acaatatttc
     7141 ctgtttttct tgttccttta aattcaggtc cttctattac tacagcttga taaatatatt
     7201 gttcatcata gtaaagaata aaatcactat aagacgacat atctaaccat tcagtgattc
     7261 gatcttccca ctcctgcacc acatcaattg aaggtgcttt atagtaacac tcaatctttc
     7321 tagtcacatt tctgtaatag cctttatcaa taatgataga atcatttcct tctcgctctc
     7381 tcaattcaat tactcgacta gctgaaacag aagcaggcct gttttgaata tacacattaa
     7441 attcagaaga ataatgttga ttaataaaaa attgtcctct cttaagttgc atataatgtt
     7501 cctcctactg caccagcatc tcgtttcatt tgtcttgtca attctgtttt aatttttgtg
     7561 gtaatcttcc gaaccattga atcaggtaaa tctccataaa catttaaatg taaatgaatt
     7621 tcttttgttt cactagcaga tatattttgc tttgtttgtg taattggtag gtgacttatt
     7681 ccatttacta caggttgaac agacggtgtt tttactaaat ttgtcatggt ttgatctaac
     7741 ttactttgct ctttatcaat accaacgata ataccttgaa caatattttt accaaccata
     7801 tcacgcatcc atcttgatgg agaatgaata tctaaagctc ctttaatcca atttttgata
     7861 ttacctgcaa tacctttgat agtatctttc aaagcattcc acttttcttt tacaccattt
     7921 ataagtccat caatgatgtt cttgccaatt tcaaataaat tgacttccct taacgaatta
     7981 aagatttctt ttacccgatt aatagcattt gaaacaccat tttttagatt tgtccatgca
     8041 ttttcagcag aattaacaat tccagtaaca atattccaga aagagtcctt aatgttattc
     8101 catgtattaa tcatggtatt ttt
//
LOCUS       DADXTG010000111         8030 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.112, whole genome shotgun sequence.
ACCESSION   DADXTG010000111 DADXTG010000000
VERSION     DADXTG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.112"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..1166
                     /locus_tag="IWI57_002169"
     CDS             12..1166
                     /locus_tag="IWI57_002169"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBC2450295.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1188..1940)
                     /locus_tag="IWI57_002170"
     CDS             complement(1188..1940)
                     /locus_tag="IWI57_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2450296.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2304..4970)
                     /locus_tag="IWI57_002171"
     CDS             complement(2304..4970)
                     /locus_tag="IWI57_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBC2450297.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5311..6762
                     /gene="cls"
                     /locus_tag="IWI57_002172"
     CDS             5311..6762
                     /gene="cls"
                     /locus_tag="IWI57_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBC2450298.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7091..7906)
                     /gene="thiD"
                     /locus_tag="IWI57_002173"
     CDS             complement(7091..7906)
                     /gene="thiD"
                     /locus_tag="IWI57_002173"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBC2450299.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2324 a         1680 c         1443 g         2583 t
ORIGIN      
        1 gaggtgttga aatgaaaata gggattgatc gtctttcctt ttttattcct aatttatatt
       61 tagatatgac agagttggca gaaagccgtg gggatgatcc tgcaaaatac catataggga
      121 ttggtcaaga ccaaatggca gtcaatcgtg caaatgaaga catcattaca ctaggagcaa
      181 atgctgccag caaaatcgta acagaaaaag accgtgagct aatcgacatg gtcatcgtcg
      241 gaacagaatc cgggattgat cattcaaaag cgagtgcggt aattatccac catttactga
      301 aaatccaatc ttttgctcgt tcttttgaag taaaagaggc ttgctacggt ggcaccgcag
      361 ctttgcacat ggcgaaagaa tatgtcaaaa atcatccaga acgaaaagta ctagtcatag
      421 caagtgatat tgctcgttac ggcttggcaa gcggtggtga agtgacgcaa ggtgtcggtg
      481 ctgttgcgat gatgatcact caaaacccgc gtattttatc gattgaagac gacagcgtat
      541 ttctgacaga agatatctat gatttctggc gtccagatta tagcgaattt cctgttgttg
      601 atggtccttt atctaattct acgtatatcg aatcattcca aaaagtttgg aatcgacata
      661 aagaattgtc gggtcgagga ctcgaagatt atcaggcgat tgctttccat attccgtata
      721 ctaagatggg aaaaaaggca ttgcaaagcg tattagacca aacagacgaa gaaaatcagg
      781 aacgtcttat ggctcgctat gaagaaagca tccgttacag ccgacgaatc ggtaatcttt
      841 acactggttc attatacctg gggctaactt ctctactgga aaattcgaaa tcactacagc
      901 caggagatcg catcggtcta ttcagctacg gttctggtgc agtaagtgaa ttttttacag
      961 gttatctgga agaaaactat caagaatatc tcttcgcaca atctcatcaa gagatgctag
     1021 attctcgaac acggatcact gttgacgaat atgaaacgat tttcagtgag acgcttcctg
     1081 aacacggaga atgtgcagaa tatacttcag atgtaccttt ttcgattaca aagatcgaaa
     1141 atgatattcg ttactacaaa atataataaa atcataaaaa gaacccttca cgagtcgtga
     1201 aaggttcttt ttttatctgc ggaccaaagg agcagtctat aggtcagctt agtcgtacta
     1261 aggatttctc gtttttcact gaatagtttg cgaatgtacc tggtcacaac aggatctttt
     1321 gcgtacgcgt catagtatag cttcatcaat tcttcagtaa ttgattcctg tgtaaaataa
     1381 gtaaaatctt ttttcttcac tttgaatcct tcatcaatta agtatgtttc aaattgatcc
     1441 attacaactg gcgatgtatt aggatcttct tctatcattc ctagttgctt ttcgatagga
     1501 taaaaaacat catcgattga ttccactact ctgattatca cgcaccattt gcttgacttt
     1561 ttcatcaatt tttctaattg aataagatgt accaatgctg gatttgctgc tgtaaaaacc
     1621 atttcatatg tctcatttga ttggataaat tcttctaacg actgtttgat aaaggaaaca
     1681 tttggcaact catttttttc tgcttctttt tttgcatttt ctagcattcc tgaagaaaaa
     1741 tcgatcaaat catagcgatt aatttctttt gctaaggaaa gaaatctgcc agatcctcca
     1801 gcaacgtctg ctgctgtttc agcaggcagg actttctccg ttttcagata attttttaca
     1861 tcttcagcaa tagttacttg cgattcttgc tggatttcat aatattcttc agcaaattca
     1921 tcccattctt tttcattcat ttgatttcct ctaatttttt gttatatgtt ttcacttttc
     1981 ggaataaatc attctcctct tttatctatt ctatctcttt tgatgaataa taaaatgatt
     2041 ggctttccgt ctatcataaa taaaaaaagg gactgtgacc aaagtcgtgt caaagtccct
     2101 ctttccgaat aaacagtgtc caatcagatg accctcagta ttagctcaaa atgctgattt
     2161 tacaacttct tccttaaacg gtgttctcct gccaactctt cggtcacaag tcacaatatt
     2221 caacaaacat gagaagctgt ttgttgaaat tgcaccttgt gactcagaat taaacggcag
     2281 gctcacaacc tcgttgattc gttttattta tttccttgta ctcgtacaat gatcaattta
     2341 gtgatttcca tcaaaacaat tgtcacaagt gcgattccca tacatgtaaa gacttctgtc
     2401 aatccaaatg ctgatgggat agagaagact tctttcagtc ctgggatcat tgttaagcag
     2461 taaagcgcgc tacatacaac tacagctagt aagacacttc tgttagagaa gaaccctgct
     2521 tggatagctg tttgtgtgtt tgaacgtgct gggaatgttt gcaatgtccg acaccaaatc
     2581 aatgttgaaa atgacatggc tacaccgaat tcaggtgaat gctgcatgcc aagccattga
     2641 gcaaagataa cagcaatccc aatcagcaca ccacgatacg ttacagaaat caatgttttg
     2701 tccgtaaaga ttcctgaatt tggatctctt ggcgggcggt tcatcgtagc aggttcagct
     2761 ttttctgtac ctaatgcaat tgctggcagt gaatcattga ctaaattgat gaacaacagt
     2821 tgtaatgctg taaatggatt ggaccagtca gcaactaagg caaagatgat cgcaatgatc
     2881 gcacctaaat ttcctgcaaa tagataagaa atagctttct tgatattatc aaacacatta
     2941 cgtcctactt ctactgcgct gacaatagaa gcaaagttat catccgtcaa taccatcgct
     3001 gctgcatctt ttgccacatc tgtacccgtt cccatagcaa ttccgatgtc tgcttgtttc
     3061 aatgctggtg cgtcatttac accatcacct gtcatagctg agatatgacc tttattttgc
     3121 caagcacgaa cgatacgaat tttattctca ggtgaaacac gtgcataaac agtgactttt
     3181 tctaaaactt cgtttagttg atcttctgtc aattcatcta gttccgtccc ggtcaaagcc
     3241 aggtttcctt cttcaaaaat cccgatttgt tttgcgatag caacagctgt tgttttgtgg
     3301 tcaccagtaa tcatgattgt tttgattcct gcacttcttg cttctgttac cgcctgagtc
     3361 acttcagcac gtggaggatc gatcatagca agcaaaccaa caaggatcaa atcgtcttca
     3421 tcttcaaatg ataaatcaaa attatcaagt ggacggtaag caaaagctag tacacgtaat
     3481 gcgcgtgttg aaaaagcttc attttgctct tggaattttt tcatcaattc atccgtcatc
     3541 ggaacgactt caccatttag caatactttt ttactgcgtt ggaagacgat atctggtccg
     3601 cctttaacaa acaagtaatt tttgttatca attgtgtgtc cagtagacat taattttcga
     3661 tctgagtcaa aaggaagttc tgcttgtctt gggtagttgt ttcggatttc ctgataaggc
     3721 tggtttaatt tttcactaaa gtcgataaac gctacttccg tagggtcgcc caatttagta
     3781 ccgtcttcac tgatcgatgc atcattcgct aaaacactga tttcgatcaa gcgacgttcg
     3841 tcagctgtcc attttgaagg gtcatttgtg aaatccccag tatcgccatt acctaaaaag
     3901 tagtcaacaa ctgtcatttt gttttgtgtc aatgtaccag tcttatctgt acagatgata
     3961 cttgtcgaac caagtgtttc aactgctgga agcttccgaa taatggcatg acgttttgcc
     4021 attttattcg ttcccaaaga taacacgatt gttacgatag actgtaatgc ttctggaata
     4081 gcagctacgg ctacggctac ggcaaacatc actgcgctga cgatatctgc agtcatatct
     4141 cctgtacctt ctccaagata aatacgaaac agttggatac ctaaaatcaa taaagacaat
     4201 gccaaaatag ccaaacttag ttttttactg aatttatcca gacctcgttg taacggagtg
     4261 acttgctcag ttgttgattc caaaaggcca gcaacttgtc cgatctccgt attattccca
     4321 gttgaagtca ctaagaattt accgcgtccg taagttacga tcgttccact gaataccata
     4381 tttgtacgat ctccgattgg taccgtactt gtgatatccg ttacttcttt ttctgcagaa
     4441 gtcgactctc ctgtcagcat accttcatct acttttaatg atcccgcatc tagtaaacgt
     4501 ccatcagcag gtacataatc gcctgcgtct aacaaaacga tatctcctac cacaagttcg
     4561 tttgccggaa tcgttttttc cttaccatca cgaatcactc gagcatcggg cgctgaaagg
     4621 tttttcaacg cttctaaaga accttctgct ttttttgttt ggacaacact gattatagaa
     4681 ttcaacatca atactgcaaa aataactaag aattcgatgt agtcacccat gaccatctga
     4741 atcagtgcga caatcagcaa tacgataacc attgcatctt tgaaagtttc taagaacaat
     4801 ttccaagttg gcttcttcgg cttttctttt agctggttca agccatctct ttctaatttt
     4861 tccttcgctt gcgtggtgct caatccatcg gttgagctct gcgtttgctg catcaacgag
     4921 tcgatcgttt ctttatagac cgcttgttgt gcttgttcct tgttttccaa atttcatccc
     4981 ccattttttg atcttggccc tagtcctgtc tgaaaaaaaa gagacctaag acaaatacgc
     5041 atttgtctta ggtctcacca tttcatacaa taccagaaaa ccatttggtt aacttactgt
     5101 tgatattatc gcaacaaacg ttgccggtta ctcccctaag gagatattca actgtactaa
     5161 tatcctacct tattaaccta caaaaagcaa ctactttagc ccgatttacc tacattttca
     5221 tgaaaggagt gaaaagtata ttcaccttgg taggcttctt tcttctatca tgctatacta
     5281 atgttattgt atgtaaggag gaacattttt gtggtatcta gtattataac cgccctttat
     5341 ctattaaatg cacttattgc tttggtggct attttgatca aaccccgaga tgtagcagcc
     5401 atttgggcat ggctcttagt attgtttgcc cttcctgggg tgggctttgt attatattta
     5461 tttttcggac gtggattaac ggataagaaa aaattctatc tccgacaaag tgacttgaaa
     5521 gaattagaaa actttcagtc ttttagagga gataccattg aacattacga tcctgacatg
     5581 ggcgataaag acaaacagca atttgttgac ttcttctctt cattaaatcg tatgccgctg
     5641 acaagaatga attctgtcac tcttctcaca gacggacaag agaaattgga ttcactgctt
     5701 caagatctaa aaaaagccaa acattcgatc catatcgaat attacgcatt tgtgacagat
     5761 aatatcggcc agcaagtctt acatgtttta gaagaaaaag ccgcagaagg cgtggaagtt
     5821 cgaatattat atgatgcatt tggctctcat ggcacaaaag caaaagattt caatcgtcta
     5881 atcaaaaatg gtggacatgt ccatacattt gttacctcac aaagggcatt acttcgtttc
     5941 cgattgaatt accatgatca ccgaaaaatc gttgtgatcg atggaaagat tagttatacc
     6001 ggtggtttca atattgccaa tcaatatgta aatacaacaa aaaaattcgg ctattggcgc
     6061 gatacgcata tacggatttt cggtgccgct tcttctttgc tccagcttcg cttcttaaca
     6121 gactggaacg tctcggtacc tgaagaaaaa aaggtcggct atcatttgaa ttatttcttt
     6181 aaaaaagcag atcgagatga atctaagctt gctgatacat ccatccagct tgtttcaagc
     6241 ggaccgaata acgaaaggga acaaatcaag ctttcattta tcaaattgat tacttctgct
     6301 aaaaaacgtg tttggataca gacaccttac cttgttcctg atgaaagtgt cattgctgct
     6361 ttaaaaatcg caactgcctc tggtgtagat gtgaaaatca tgattcccaa caaaccggat
     6421 catcctttta tttatcgagc aacacaatat tatgctcggc agctgatcaa ggaaaatgta
     6481 caaatccttg tctatgagaa cggcttcctc catgcaaaaa cattgataat ggatgatgaa
     6541 atctgcatgg taggttcagc aaatcaagat attcgtagct accgattgaa ttttgaaaca
     6601 agtgctgtca tttacgatcc tgagttttta gaagaacttg ctactcagtt caaagaagat
     6661 gagacacatt gttcatccat gacaactgaa acagtcaagg aaatgtctaa ctggctatta
     6721 ttcaagcaac aaatttctcg attattttct ccaatcctat aattttttta ttcaagtaga
     6781 gaaaaatata ctaagattaa agggactgtg acaaaaatct tgtcacagtc ccttcttccg
     6841 aacaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga
     6901 gaagctgttt tttgaacttg ttccttgtga ctcggagctg gacggcaggc tcacgacctc
     6961 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac
     7021 ataagaagct gtttttactt ggctgttctt attacttgat gcagggcaac tttttcacaa
     7081 cttctttctt ttactctaat tcaccagctg cccaatgatt gatggggcca tatttgtgcc
     7141 ctacatttat ttcttcacga attgcacggt ttacatagtc ttttgccaat cggatcgctt
     7201 gttcaactgt ttttcctttc gctaactcag ctgcaataca tgcagatagc gaatcgccag
     7261 ttccgttgat ccggtcggta ggataaaatg gtgcatttaa ccaaaatgat tttcctgatt
     7321 cgaacaatac gtagtcattg actgtttctt gctgcgcatt tttagcatga tgtcctttga
     7381 tcataacgtt ttttgcaccc atatcttgca gttcttttgc tgcttgcaca aaatcttctt
     7441 cagctgctaa tgtccgtcca gtcagctttt ctgcttcata aaaattaggt gtcagtactt
     7501 cggccaaagg gacaagcttt tctttcaagc tttgcaaagc agattcttct agtaaaagat
     7561 ttccatgttt cgttacgatt acgggatcca cgactaatgg tccaaaatca tatttttggt
     7621 aattttttac aactgtctca atcaactttg aatctgctag catacctgtt ttagctgcac
     7681 gtatttgaaa gtccttcgca atcagttcaa attctttgtc gataaaatct gttggcaatg
     7741 tcacacttgc tccgattccg taagaattac ctgcaacaca tgcggtcatg acagaaacac
     7801 cgtaaacatt tcttgtaaag aatgtatgca tatctgcttg catccctgcg cttccatcgc
     7861 tatctgatcc tgcgatcgtc attgcttgta catactgatc ttccatcaaa tcgattcctc
     7921 catttttata aaaagatttt tcataatact gttactgttt ctatcatatg tttttcaaag
     7981 tatccctcag taaatatacc actacctttt cttggtaatc aaatttattt
//
LOCUS       DADXTG010000112         7880 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.113, whole genome shotgun sequence.
ACCESSION   DADXTG010000112 DADXTG010000000
VERSION     DADXTG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.113"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(97..2268)
                     /gene="copB"
                     /locus_tag="IWI57_002174"
     CDS             complement(97..2268)
                     /gene="copB"
                     /locus_tag="IWI57_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBC2450300.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2288..4474)
                     /locus_tag="IWI57_002175"
     CDS             complement(2288..4474)
                     /locus_tag="IWI57_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBC2450301.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4474..4683)
                     /gene="copZ"
                     /locus_tag="IWI57_002176"
     CDS             complement(4474..4683)
                     /gene="copZ"
                     /locus_tag="IWI57_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBC2450302.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4696..5136)
                     /locus_tag="IWI57_002177"
     CDS             complement(4696..5136)
                     /locus_tag="IWI57_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBC2450303.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5210..5749)
                     /locus_tag="IWI57_002178"
     CDS             complement(5210..5749)
                     /locus_tag="IWI57_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBC2450304.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6364..7830)
                     /locus_tag="IWI57_002179"
     CDS             complement(6364..7830)
                     /locus_tag="IWI57_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBC2450305.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2141 a         1742 c         1381 g         2616 t
ORIGIN      
        1 acaaaaaatc tgagccagaa tgaggttcat tctgacccag atttgatttt gtacaaccca
       61 tcagtcctta ttgacaaaga gccccttttt tcattattat ttgattttca atgtcaaagc
      121 atttagagct acaactaccg tacttaacga catcaagaca gcaccaactg ctggacttaa
      181 tatgatacca attggcgcaa gaattccggc tgctagcgga attgcaagaa tattgtaacc
      241 tgccccccac cagaggttct ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa
      301 atgcaaaata tcttttggat cgctgtttgt caatacgaca tccgcagaat cgattgcgat
      361 atctgtccct gcaccgattg ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt
      421 gatcccatca cccaccatga ttactttttt cccttggtca aggtatttct ggacaacagc
      481 ttctttatca tctggtaata aaccgccata atattcttcg atccctaaat aatctgctac
      541 tgcttgggca gcgtttttat tgtcaccagt aagcatgaca ggagtgattt ttctttcttt
      601 caatgttcgg ataaattctt ttgcttctgt ttttactacg tcacctaaag caacgattgc
      661 agctagatga ttttctaaaa tcaggaagct gatggtattt ccttgttctt ggtaaggttt
      721 caataatgct tgttctacgt ttaatccgag acgctcaact tctttttcat taactatttt
      781 cacttgttgt tttttgactg ttgcctctag cccgactcct gaaagatttt ttagattttc
      841 agcttgataa ggcttgattt catgttcttt caaataattc ataatgccaa cagccaatgg
      901 atgattggca ttcgcttcca aggctcctag atattgaagg atttcatttt tattgaacgc
      961 atcatcaagt acttcgacac ccgttacagt aaattttcct tctgttagtg ttcctgtttt
     1021 gtccagcatg atcacatcta gatcattggc ttgctccagt gcattgcggt ttttcaaaag
     1081 taatccgttt ttagctgcta ttgatgtgga gcgagcaaca actaatggga tagctaaacc
     1141 tagagcatga ggacaggcga tgataaagac tgttaccata cgttctaatg cattaggaag
     1201 atctgtcaag aacaaccagg cgatgaatgc caggataccc acaattaatg cgacataaaa
     1261 cagccatttt gccactttgt cagataatga ctctagcttg gatttttctc cttgtgcttt
     1321 tcgcaccatt tccataacct tcgctagata accattttca ccagttccag ttacttcgat
     1381 ttcgatcgtt ccatccccat tgatagaacc gccaatcact ttgtcattgg cttgtttttt
     1441 gattcctctt gattcgccag tcacagctga ttcgtcaaca attgtttcac ctttcaagat
     1501 tacaccatct gttggcattt tatcaccaga ccggacgatc aaacgatcac cttcgtttac
     1561 ttctttcaat gaaactgtct cttcttttcc atctttcgtc aagcgtttca ctgattctgg
     1621 cagtaattct gctagttttt gtaaagcatt gctggcattc gacacagcat tcatttcaat
     1681 ccagtgacct agtagcataa taacgatcaa agtagccaat tcccagaaga aatccatgac
     1741 gtgtgtatgt ggattgagca gatttgcgat aaacgaatag acactataaa tatatgcaac
     1801 tgtaatcccc atcgcgatca gtgtcatcat agcaggactt ttttgtttca attccatctt
     1861 agctccactt aaaaatggct gaccgccata gataaatagt atagttgcta agatcaatac
     1921 gagccaatca gaaccaggaa acgttacttg aaatggaaat tccattccca tcattggtga
     1981 aaaaagaatg attgggatag ctaggataag cgatagccaa aatttttgtt tgaaattccc
     2041 catgtgcatg gaatgatcca tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat
     2101 ttgtttgctg tttgctatat ctccatggtt atgtcccatg tgcatggaat gatccatctg
     2161 actatggtca tgtttcatct ccatctgttt ttcatgagat tctgtacttt ctttgtttac
     2221 tacttctttt ttatgctcat gttttttctc catatcgttc ccattcattt ttactccatc
     2281 ccttctctta ctttttcatg tgtcgattta agctcaggga gttcaacaat acactgacgg
     2341 aactaaacgc catcgctcct cctgcaatga ttggattcaa aaagcctagt gcggcaaaag
     2401 gaataccaat cgtattatag acaaaagccc agaaaagatt ttgtttgatt tttctcaatg
     2461 tcaacctaga caactcgatt gtttgtgcaa tggatgccaa actgctattc atcagcgtca
     2521 catcggctgt ttccatcgca atgtccgttc cacttcccat tgccataccg acatcagcta
     2581 atgctaaggc cggtgcatca ttgattccat cgcctgccat accgactttt ttgccatctt
     2641 tctgaagttt ttctacgtag gctgctttat cctctggaag tacttcagca aagatatgct
     2701 ttggatcaat accaacttgt ttaccaatcg tttcggctgc cagtttgttg tctcctgtca
     2761 gcataaaaac atctaatcct ttattctgga gctgcttgat tgcttgtttg gcttctagtt
     2821 tgatttgatc agccacggca atccacccga tcacttgctt ttcatcagct aaaaacatga
     2881 ctgtttttcc ctgctgttct aattctaacg cgtgttcttg gtattcatca aatgataggt
     2941 tcaattcaat taatcgttta cgtgtacccg caaaatagcg tactccatta atcgtaccgc
     3001 tgatcccagc acctgggtgg gctgtaaaat caatcatatc gtatgttgct gcttcttgca
     3061 gttttccgta ttccacgatt gctttaccta gtggatgttc agaagcttgt tctaaagtat
     3121 aaaaaataga taggacttct tttgaaccga ctagatctgt aactttgggc tttccttctg
     3181 taatcgttcc agttttatcc aaaacaatgc tgtctaagtg agcagctgct tctaatgctt
     3241 ctccgccttt aattaaaatt ccatttcgtg caccaacacc cgttcctacc ataatagctg
     3301 ttggggtcgc taaacctaat gcacatggac acgcaatgac taatacagag acactatgga
     3361 tcaatgcgag ttgccaatca ccagtgatca aacctgtagc tatgagcgtg ataaacgcga
     3421 tgactaatac gatcggaaca aaaatccctg agatccgatc tgcgattttt tggattgggg
     3481 ctttgctccc ttgagcatct tccaccattt gaatgatttg cgctaatact gtctgcttgc
     3541 cgacttgtgt gacttccatg tgtagcagac cattcgtatt gattgtcccg ccaaaaagcg
     3601 tgtcatccgg attcttttct acaggaagat tttcaccagt aagcatactt tcatctatcg
     3661 cactgcttcc tgaaataatt cgaccatctg ctggaatctg ctcaccagga cggatgacta
     3721 gttgatcacc aacgactact tcttcaatcg ctaacgtttc ttctttcccg tttcttatca
     3781 cttgggcggt ttttgtttga agagacatca attgtttgat tgcattacct gttttcgttt
     3841 ttgctgtatg ttccaaatat ttgccgagca aaatcagcgt gatgatcata ctgctgcttt
     3901 caaaataaag atcttggggg tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg
     3961 cagccgatgt tccaattgct accaatacat ccatatttgg tgctttcgtc ttgattgcat
     4021 ggtacgcacc tttataaaaa cgagcaccga cataaaattg gacaggagca gacagcacta
     4081 actggacgat tggaaaatga aaaaagtgaa caatcgccgc atggcttcct aacatcatag
     4141 caatcattga caacatcaac ggcaaagtca aaaccgtact caatataaga tcgaatagca
     4201 tttttttcaa atacgcttgc ttttcttctg caattttttg tttgtgggct tcatcgtaca
     4261 aaattgcacc ataaccgatg ttttctacgc tttggattaa tttttcagtg gtcgtccctt
     4321 catatttgac acttgctttt tcagtggcca agttgactgt ggcattgacc acaccaggtt
     4381 gtccgttcag ctctttttcg atcctagcag aacaatttgc acaagtcata cctgtaatga
     4441 cgaacgtatc cattttggtt tctgctgttg ccatcaaatc acctctgctt tataacccaa
     4501 tccattgatt gcttgacaaa tcttttcgga ttgaactttt gtttcatcaa acttgaccgt
     4561 accgtttgct tttttcaaat ttactttcac tttttgcacg ccgtcaagtg cgcttactgt
     4621 ttcttctact cttgctgcac aatgattgca gctcatacct tcgattgaaa attgttgttt
     4681 cattttttct tcctcctatt gatgttgttt acattcacat tgacctggga tacagttaca
     4741 ttcgatcgta tctactggtt gcttttgttc cagttgtttc aaaatctcat tgatatcttc
     4801 ttgagtcaaa gtggcttctt ctactagatc cgcaatcgtc tctccaatcc tttttgcaca
     4861 gatatgggaa aaaagatttt ccgtcgcact gcgaactgtt tccatctccg agacagctgg
     4921 atgataaatg aatttttttc cttcttgttc tgtccagatc acttcttttt ttaccaaacg
     4981 tcccagcaaa gttttgatcg ttgctggttt ccaatccatc gaatcagcta agatctgcgt
     5041 gatttcttgg gctgtcgtct gtccaagtgt ccaaataaca cgcatgatct cccactctga
     5101 atcactgatc tttactggcg ttttgacttt ttccatttat ttcacacctt tcgtttacaa
     5161 atgtaatcat tagcttatga atacagatta cgcttgtaaa caaaaactgt caattatttt
     5221 tgctctctat tttcaattta gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga
     5281 tcagtttatt tcttgcatta gctacttgga tcagctgctg tattttcggt tcattgtttt
     5341 cttcaaaatg ccagataaaa gataaaaatt ctaagtattc cctgtttagt ttctctgaaa
     5401 atgattcagg catatctggc cgagattttt tgtttatctt tctttttaga gaacgccaaa
     5461 ctactcggta gatggcgaga taacgcggca ctttaagcca aatgatctta tctgcttctt
     5521 taatcctttc ctctaatgta gaagtataat ttccttcaac aatccatgac ggattagata
     5581 ccataaacga ttgctgttct tgcaaaaacc acttttttgc ctgaatactg tagtctgttg
     5641 tatgccataa aaggtctagc ggcagtagcg gaaagtcggt gatttctttg atcttccttg
     5701 cgagggtcga ttttcccgac ccactttgtc caatcacgat gatcttcatt cttctccctc
     5761 acttctcttt ggaatttggt tcgatcatac cacttataac gccgcaaaaa aacaagggac
     5821 tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa tggtgttcaa tcagctgacc
     5881 ttcagcatta actcaaaaaa cggcaaaata tgaggagcta ttttttcgtt tgttcctcta
     5941 atactcaggt caaatcgctg attttacaac ctcttcctta aacggtgttc tcctgccaac
     6001 acctcggtca caaggcacaa tagtcaacaa acatgagaag ttgttttttg aaattgctcc
     6061 ttgtgactcg gagttaaacg gcaggctcac tacctctttc ttaaacggtg ttcaaaagct
     6121 tgcatctgcg gtaataagcc cagcaaaacg aaaaatatga ggggctattt ttgtttggct
     6181 gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccagaa
     6241 gtagcttctg cgaaaaacaa gccgaaactt cacaaaaatt tgagaagcaa ttttcgtaaa
     6301 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcttgtaac aagtatttct
     6361 attttaaggc tgaaccactg tttctggttc agtgatttct ttgtttttgt ctcgacgagc
     6421 aattgctggc aagatcatac ctaataagat caagacaatt ggtgtgatga tattcgtgaa
     6481 taattggaac caccatgctt taggatcagc agtataatct acttttggca ccattcctag
     6541 gatacatgca aacgcagtga aggcaaagca ccaagcacca aagataaaac cgatttttgg
     6601 atttttaaca aatttatatt cagaattgaa tttcttataa gctttattca acatcatata
     6661 tgccaagaac acccataggt aacgcattgg catgacaact gagttcaagt tcgtcaacca
     6721 ttttactaat tcattcatat ctttgatacc aaagatcggc aacaaaataa tgattcctac
     6781 tagtatacct gtcagaagat aaccattcac caatgtacct ttacgattac gtttacgtag
     6841 ccagtttggt acaaattctg gatccgcatc tgctaacaag atttttagtg gtgcatcaat
     6901 tgaaaaggct aatgcggaga tttgtccaat catgtttgtc aatgcgtaaa tgatcatcaa
     6961 tgagttaccg ataccatagt aattacctaa aatttgaaaa gcactgtatg ccccatttgc
     7021 catcaagtct ttaggaatat tatcgctagc aaacagcata cccattgcga ctgagcctaa
     7081 tacagcacaa atccctacca ttgctgctaa gaagatcatc cctttaggaa attcctttgc
     7141 aggatttctt gtagagttga cataagggga tattttttcc gctcctccaa ctgcaaatac
     7201 tagcatcgaa acggttgtga aataactgaa atcaaatgtt ggaatatatg ttttcacttg
     7261 attcatatgt ggtgtggcaa attccatcgt tggattgatg aacggtgccg ttacagctaa
     7321 aagaataaat aagatcgaca tgacgaacat cgctgttccg gccagtccgc caatcacttt
     7381 caaagtagaa agtccttttg ttgataaata taaaaaggca agaaatataa taagtgaaat
     7441 aactgctacc atttgtactg acatcgtatt tacgatattt ccatttcctt ggactgccca
     7501 accgccagca atcaggattg cttgaggctt ttgtgccaaa taaggaatat ggacgaccca
     7561 gtaagtccaa gcggcatagt atgccagtcg tttattagat gtcgtatttt cgacccatga
     7621 actgacgcct cctttactat ccttgaaggc tgaccctaat tgtccaacga tcaatgcgta
     7681 tggaataaaa taaatggcca aaatcaatag ccaagaggtg acaactgata ctccttgctg
     7741 agcatagtta ttaactacat tccccatgcc ccaaaccatg ttaaatgcaa ttaacgcaac
     7801 agtaaaccag cgtaattgtt tgttgttcat tcgttctctc ctttatgata aaaatttgtt
     7861 atccagatgt aatcatattc
//
LOCUS       DADXTG010000113         7775 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.114, whole genome shotgun sequence.
ACCESSION   DADXTG010000113 DADXTG010000000
VERSION     DADXTG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.114"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(203..688)
                     /locus_tag="IWI57_002180"
     CDS             complement(203..688)
                     /locus_tag="IWI57_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBC2450306.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    778..1123
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1168..1809
                     /locus_tag="IWI57_002181"
     CDS             1168..1809
                     /locus_tag="IWI57_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBC2450307.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1828..2463
                     /locus_tag="IWI57_002182"
     CDS             1828..2463
                     /locus_tag="IWI57_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBC2450308.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2481..3296
                     /locus_tag="IWI57_002183"
     CDS             2481..3296
                     /locus_tag="IWI57_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2450309.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3615..5051
                     /locus_tag="IWI57_002184"
     CDS             3615..5051
                     /locus_tag="IWI57_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBC2450310.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5240..7687
                     /locus_tag="IWI57_002185"
     CDS             5240..7687
                     /locus_tag="IWI57_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBC2450311.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         2600 a         1311 c         1735 g         2129 t
ORIGIN      
        1 aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta
       61 actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat
      121 tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg
      181 aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa
      241 caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat
      301 gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct
      361 ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag
      421 gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt
      481 ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg
      541 atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg
      601 acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat
      661 aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat
      721 cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa
      781 atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa
      841 gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga
      901 aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt
      961 tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga
     1021 tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg
     1081 aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat
     1141 ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta
     1201 ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca
     1261 ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt
     1321 caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt
     1381 tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt
     1441 atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct
     1501 caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa
     1561 atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac
     1621 ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg
     1681 aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat
     1741 cttacattga ctggtattct aacaatcgta atgaaacaag ttgaaggaaa attaaattat
     1801 taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt
     1861 ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct
     1921 atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa
     1981 agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa
     2041 atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat
     2101 atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac
     2161 accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat
     2221 ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg
     2281 aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag
     2341 caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc
     2401 catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat
     2461 taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc
     2521 ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca
     2581 acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag
     2641 ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat
     2701 atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca
     2761 atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag
     2821 tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag
     2881 gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg
     2941 gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg
     3001 ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg
     3061 gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag
     3121 acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt
     3181 cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg
     3241 aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa
     3301 taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct
     3361 gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt
     3421 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
     3481 caaaattttt cttactactc gaaactaagc gactttttca caacctcctt tacataattt
     3541 tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg
     3601 aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg
     3661 gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat
     3721 cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt
     3781 tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc
     3841 tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag
     3901 tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata
     3961 ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata
     4021 atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg
     4081 aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt
     4141 cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg
     4201 aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag
     4261 aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta
     4321 atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg
     4381 atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat
     4441 gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc
     4501 ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg
     4561 caggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa
     4621 tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc
     4681 cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga
     4741 ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg
     4801 actatcgaat caattactta caaagccacg tcaaagccat caaagaagca atttcggatg
     4861 gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg
     4921 gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt
     4981 tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag
     5041 agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt
     5101 cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa
     5161 gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg
     5221 atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac
     5281 gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt
     5341 tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat
     5401 tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc
     5461 cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg
     5521 ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt
     5581 tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt tgttagccag
     5641 tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct
     5701 ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt
     5761 actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat
     5821 ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg
     5881 aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg
     5941 cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt
     6001 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     6061 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     6121 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     6181 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     6241 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     6301 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     6361 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     6421 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     6481 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     6541 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     6601 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     6661 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     6721 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     6781 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     6841 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     6901 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     6961 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     7021 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     7081 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     7141 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     7201 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     7261 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     7321 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     7381 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     7441 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     7501 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     7561 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     7621 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
     7681 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
     7741 gatgatacca aaagaaaaaa tggttttttt ccatc
//
LOCUS       DADXTG010000114         7636 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.115, whole genome shotgun sequence.
ACCESSION   DADXTG010000114 DADXTG010000000
VERSION     DADXTG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.115"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            39..1175
                     /locus_tag="IWI57_002186"
     CDS             39..1175
                     /locus_tag="IWI57_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBC2450312.1"
                     /translation="METTEAVQDDQDLAKMYTPGVAELSLMIEKNHALARELTISGKL
                     VAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAIPLAIEQKSVDEIVQTIV
                     NLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEGTAIVVLAGLINAAKIQR
                     KTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRLKKQDSTLNKYQRELLDH
                     IHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDNPIIFALANPVPEITPDL
                     AKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKVDLSLQLEVAKQLAAKVA
                     EPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAENIK"
     gene            1156..2460
                     /locus_tag="IWI57_002187"
     CDS             1156..2460
                     /locus_tag="IWI57_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBC2450313.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2486..3499
                     /gene="citC"
                     /locus_tag="IWI57_002188"
     CDS             2486..3499
                     /gene="citC"
                     /locus_tag="IWI57_002188"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBC2450314.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3503..3796
                     /gene="citD"
                     /locus_tag="IWI57_002189"
     CDS             3503..3796
                     /gene="citD"
                     /locus_tag="IWI57_002189"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBC2450315.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3801..4706
                     /gene="citE"
                     /locus_tag="IWI57_002190"
     CDS             3801..4706
                     /gene="citE"
                     /locus_tag="IWI57_002190"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBC2450316.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4699..6258
                     /gene="citF"
                     /locus_tag="IWI57_002191"
     CDS             4699..6258
                     /gene="citF"
                     /locus_tag="IWI57_002191"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBC2450317.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6255..6812
                     /gene="citX"
                     /locus_tag="IWI57_002192"
     CDS             6255..6812
                     /gene="citX"
                     /locus_tag="IWI57_002192"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBC2450318.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6805..>7636
                     /gene="citG"
                     /locus_tag="IWI57_002193"
     CDS             6805..>7636
                     /gene="citG"
                     /locus_tag="IWI57_002193"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBC2450319.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     L"
BASE COUNT         2608 a         1221 c         1588 g         2219 t
ORIGIN      
        1 ttttagaaat ccataaaaaa aatgtaggtg ttttacggat ggaaacaaca gaagccgtgc
       61 aagatgatca agatttagca aaaatgtaca ccccaggtgt agcagaacta agtttaatga
      121 ttgagaaaaa tcatgctttg gcaagagaat taacaattag tggtaaatta gtggccgtta
      181 ttacagacgg gtcggcggta ttaggtcttg gaaatgtagg aaaccaagct ggattaccaa
      241 tcgtcgaagg gaaagcgctg ctttataaaa atttagcagg tgtcaatgcg attccattag
      301 ccatcgaaca aaaatcagta gacgagattg tacaaacaat tgtcaatttg caaaatagtt
      361 ttgcagggat ccatttagaa gatattgcag caccgaagtg ttttgagatt gaagaaaaat
      421 tacaagaaaa actttctatt cctgtgtatc atgacgatca agaaggaaca gcgatagttg
      481 ttttagctgg attgatcaat gctgctaaga tccaaaggaa aacattaact gaacttcgag
      541 ttttgatcaa cggcatggga gcatctgggg ttgcaacagc tagactatta atcgctgctg
      601 gtatcaaaaa tctaacattg gtggataaac aagggagatt aaagaagcaa gatagtacat
      661 taaataaata tcaaagagag ttgttggatc atatacatga tacaaataat aatgcaagcg
      721 ttttggaaga tgcaatcgtt ggacaagatg tttttatcgg tctttcaata ggaaatgttt
      781 taacgcctga aatgattgga aaaatgaatg ataatccgat tatttttgct ttagccaatc
      841 cagtaccaga gatcacaccc gatttagcaa aagaatctgg tgcaaagcta atcgctacag
      901 gttcttcgaa atatcctaac caagtaaaca atattttagt atttcccgga ttgttcaagg
      961 gactattact atcgaaagcg aataaagtcg atctttcatt acaacttgaa gtagccaaac
     1021 aattagctgc taaggtcgca gagcctacgg tagaaaaatt catccctaat gtttttgatg
     1081 aaggagtcgc aaacgtcgtt tcacaggcag ttttggatta tttacaaaaa ctaaaagaaa
     1141 acgaggataa tatacatgct gaaaacatta aatagaatta aaattggttt tattccttta
     1201 tctctttata tcattttggt actcgcgtat cttgcaatga cgataagcaa tatcgttcca
     1261 gaaaatgata tgttagtttc tttgggtatt atgactgttt ttgcttattt attagaagaa
     1321 attggtaaaa ctattcctat tttaaaagat ttaggtggta aagtattagt tgttacattt
     1381 ttgcctgcat ttctagtcta taaacattgg ctacctcaaa gtactgtaac tgtggtttct
     1441 gattttatga ataataacaa tttcttgatg ttctttatca cactactagt tgtcggcagt
     1501 attgcttcaa tgaatcggca aacattggtt aaagcaactt cacgtattat cattatttta
     1561 attttatgcg atgtgattgg ttctttgata ggaacaggtg tgggaatact ccttgggcta
     1621 tctgcttgga atgcgtattt ctttatcgtt gcgccgatta tggctggtgg tgttggggaa
     1681 ggagcgatgc cattatcgat tggttatgca gcaatattat ctggtatggg acaaaatgat
     1741 atctttggga agattctacc gtgtgtgatg ttgggaagtt tagtcgcagt tctactgtct
     1801 ggtatgctca aaaaaattgg agagaaatac ccacaattaa atggtcaggg taatttgatc
     1861 gatggggatg atgaacattt agagaacttg aagggttcag ctaaaccagt caatattgaa
     1921 aaaacattgt tagcaggcgt ggtagcaata acgatttatt ttgttgctgt ctatgttgag
     1981 cacttattaa aatcactcct tggcttttct attccttcac ctgtgatctt attagtctct
     2041 cttatggggg ctaaaatgct tggttttatc cctaaaaata tcgaagaagg tggaaattcg
     2101 ttgtatggat tcactgttcg tgggatcaca cctcccttac tttttggcgt aggggttgca
     2161 aaaacagatt ggttagagct gatcgaagta tttaaaaatc ctgcaacgat agtaacaatc
     2221 attgtgacgg tagtagctat tgtcgctaca gcttttatcg ccgcaaaatt tttaaaaatg
     2281 tacccagttg atactgccat cgcagtttca tgttgtagcg gacaaggcgg tacaggtgct
     2341 ttagctatct tagctgcggg tgatcgcatg gaattaatgc catttgcaca agttgctgtt
     2401 cgactagggg gtgctatgac cgtaacgttc gctatctttt taatgggtct gctatcataa
     2461 gaacataaag aaaggacgtg accatgtgtt tgatcaaaca gatatccaac gattattttt
     2521 aaaaaataac tatatcaaag accaatggga aaatcttttg cttgattcag gaatacaaaa
     2581 gaagcaaatc gaagattttc aacatttaga tcaaacattg ggtatatacc ataaagaaaa
     2641 gttagtagga acagtatctt atcaaaacaa cgtgattaaa tatatcgccg tgtctgaaaa
     2701 atataaagat ggtggacata catttaatgt actagtctct gcaataattc aggcaatgag
     2761 tcaacaagag atttttcatt atattgtttt cacgaaaccg cgctatcaaa aaagttttaa
     2821 ctttttaggt tttgaaacaa tcgtatctac cccaaaaata gctgtattag aaatgggaga
     2881 tagttctata aaacagtacc tagagaggat tcctaaagtg aatggtgaaa aaaaagcagc
     2941 aattgtcatg aatgcgaacc catttacaga agggcattat tatttagtaa aacaagctgc
     3001 caataacaac gattgggtat atgtatttgt actcgaaaaa gaacaaaatc ttttttcgac
     3061 gaaggaccga ctcaaattag ttcaacaagg attacaaaaa ctgaaaaatg tcattgttct
     3121 atcaggtggc gagtatatta ttagccctgc aacgtttccc acctattttt tacgagaaga
     3181 tgatgaaata gcaaaggagc aaatggtcgt agatgctact ttattcaaag aaagaattgc
     3241 cacttattta gatatctctg ctagatatgt gggagaagaa ccactttccc caatgactcg
     3301 ttcatataac gacatactgg aaacgatttt accccctgaa ataaaagttc atgtccttgc
     3361 aaggaagaaa acggaacaac accaagttat ttctgcttct caagttcgta aagcgtatct
     3421 tgctgggcaa ttggaaaaaa taaaatatat ggttccagaa acaacctatc aatacttaaa
     3481 aaataaaagg gagcgttaag taatggaaat aaataaatca gcgattgctg gaacattaga
     3541 gtcaagtgat gtccaaatca tgatttcacc gaatacagaa ggaatagaaa tcgatttaga
     3601 cagtcaagtg attgagcaat ttggaaaaca aatcaaaaaa gttatctatg acacactagc
     3661 agtttatcag attaaaaacg taaaactcaa ggtagtagat aaaggggcat tagattgcgt
     3721 tattgttgca cgattaaaaa cagcgatcca tcgttcctta ggtatccaac accaagaaac
     3781 accgtgggag gtttaattag atgatgaaag aacgtttgag aagaacaatg atgtttgttc
     3841 caggaaataa tccagcgatg atcaaagatg caggtattta tggtgctgac tcgattatgt
     3901 tcgacctgga agatgcagtg tctatggcag aaaaagacag cgcaagaatg cttgtggctg
     3961 aggcattgaa aacagtggat tatggtgaca cagaattaat tgttcgaata aatgacctgc
     4021 actcagaatt tggacgtgca gacatttttg caatggttaa agccggcgcg catgtattac
     4081 gtttacctaa aacagaaacc tctgaagata taacagaagt agaaaaagtg attatagaag
     4141 cagaaaacta tcataatgtt ccgatagggc gaacattgat gatggcagcc attgaaggtg
     4201 cttctggtgt attgaacgca cctgcgatcg ccaaagcatc tgatcgattg attggaatcg
     4261 ccattggggc agaagactat gtgacaaata tgaaaaccaa acgatatcca gatggaaacg
     4321 aattgttttt tgctcgcagt atgatcgttc atgctgcacg tgcagcaggc atttccgcat
     4381 ttgacactgt ttactctgat gtggatgata tggaaggatt tttaaacgaa gtacgtttaa
     4441 tccatcaatt aggttttgat ggaaaatctt gtatcaatcc acggcaaatt ccagtagtca
     4501 atactgttta tcagccgtca atcaaagaaa tcaaacgatc actagacatc atctcggcaa
     4561 tcgaagaagc aaaagaaaaa ggttctggcg taatttcttt aaatggaaaa atgatcgata
     4621 aaccaattgt agaacgtgca caacggacat tgatgttagc aaaagcatca cgattgattg
     4681 atgaggaggg aaatatccat gcataataaa caaagacgta gcattccatc aagtgtttta
     4741 acaaagtata aaaaagaatt ccaaagtgtt tcattatcgc cacaaacgat tcaacgttgt
     4801 gcaccaaaag taacgactgt aactaatcag acaaataaat tgatgcactc gatcaaagaa
     4861 gctatagaaa aatcgggctt aaaagatgga atgacgattt catttcatca tcatttgaga
     4921 gaaggtgatt ttgtttttaa ccaagtgatg gccgtgatcc gtgaaatggg catcaaagaa
     4981 ttgaccttag caccaagttc tttaacgaac actatgaatg agatggttat tgaatgcatc
     5041 aaggacggta cagtaactca tatcacaagc agtgggatgc gcggcagctt aggtgaattt
     5101 atttctcatg gaggaatgga caatcctgtg attttacgtt ctcatggtaa tcgtccacgt
     5161 gcaattgaaa gtggtgaatt gaaaatcgat gttgcatttc taggagtacc aacatctgat
     5221 gattatggaa atgctaatgc gactgttgga aaatccgttt ttggttcatt agggtatgca
     5281 aaagtagatg cacaatatgc agataaagtc attcttctaa ctgatcattt agttccttac
     5341 cctaatacgc ccattagtat tccgcaaaca caagtcgact atgtcgtgga agtagaagca
     5401 attggtgatc cgaataaaat cggtgctggt gctacacggt ttactaaaaa cccaaaagaa
     5461 ttaaaaattg ctgaaatggt caaagatgtc attgttcact caccttattt caaagatggc
     5521 ttttcttttc aaacaggaac gggtggtgca gctttagcag taacgcgtta cttagctagt
     5581 gaaatggaag aaaaacagat caaagcttct tttgcattag gtggcatcac taaaccaacg
     5641 gttgatttat tggaaaaagg tcttgttgaa aaagtgttag atgtccaaga ctttgacaaa
     5701 ggagcagctg aaggaatggc gcggcttgag aaccaacagg agatcgatgc ttccttttat
     5761 gctgatccaa gtaataaagg agcggttgtt aaccaattag atgtcgctat tttatctgca
     5821 ttagaaattg acacaaactt caatgtaaat gttatgactg gttcagatgg tgtgctaaga
     5881 ggtgcgattg gtggacatca agatgcagca acggctaaac ttactatcat ctcagcgccg
     5941 ttagtacgtg gccgaatacc tacagtagta aatgatgtta ctacattgat cacaccaggg
     6001 aaaagtatcg atgtattagt cacagaagta ggggttgcta tcaatcccca acgtaaagat
     6061 ctaatagcta tttttgaaag aattccacaa attcctgttt ttacaataga agaattacag
     6121 caaaaagcag aaaaaatagt aggcatcccg gaacctttgc aattcacaga tcggatagtt
     6181 gcctatgtag aataccgaga tggttcaatt ttagatgtcg ttcgtcaggt gaaagaattt
     6241 gaagaagaga gatcatgatg tttgatactg gagaaattca agaaatcgag gatattttga
     6301 tccataaaga acaacgtgct aacttgcaaa gcaacctgct ttccaagtat tcaaatagtt
     6361 cattgatggt ctttacattg aatattcctg ggccgatcaa aaataatcca atgattgagc
     6421 aggtatttcg tcagggacag cagtcaattc agcaaatgtt tttaaaaaaa aaggtggaaa
     6481 tgacgtacac aaaagaaatc aatttgccaa gtggattgga ctggtacgta atagtaacag
     6541 aagcaccttt gaatctcaaa aaatggttgg tcacgttaga agagcaagac gcattaggta
     6601 gactttttga tttagatgtc ttgttcaaaa aaggagagca gataacaatt gtcagtcgcc
     6661 aagctcttaa tcatggggaa cgtccttgtt ttatttgtgg gaaatcagca aaggtatgtg
     6721 cacgtaatcg tacacatact gtgaaagaaa tgcaaaagaa aatcgaacaa atagtagccg
     6781 attccttaaa aggagcattg aaacatgcat gataatattc aaaaaatagc acaagaagca
     6841 caattagcga tgctttatga agttacatgt atcaataaac caggattggt tgatccagta
     6901 gaccccgggt ctcatcaaga tatggacatt tttacttttc tacaaagtag tgtagttttg
     6961 actccctatt ttgaagaatt tatccaaaca ggattggacc gacaacatca accaattgaa
     7021 gaaaccttta tggcgattcg acagattggt ttagaggctg aaactgcgat gttctcagtg
     7081 acaaatggta taaatacaca taaaggtgct atcttttcat tggggatttt tcttgctatt
     7141 tgtggccggt taactatttg gaaagttcct tgtaaaaaaa gtttctttca aaaagaaatt
     7201 caaaaaatga cgaaaaatct acttaacgat tttgggaaga ttgatcaaac aaaaccaaaa
     7261 gcatggactt ggggagaata tctatttgtt aaccatggat tgacagggat acgaggggaa
     7321 gccaaaagag gttatccttg tgtttttgac cgaggattgc cttattatca agcatatcaa
     7381 gggtcgcaac aagataagat gatagacact ttactttttc tatcaatgga agtagaagat
     7441 accaatttga tcaaacgaag taataataga catgtttttg aagagtatca attacttatt
     7501 cgtccttatt ttgaattagg gggtgtaaaa accatacaag gtaaaaaata tttggattac
     7561 ctaaatcagc aatttaaaaa gaaaaattgg agtattggtg gaagtgcaga cttattgatc
     7621 cttacaatct ttttag
//
LOCUS       DADXTG010000115         7598 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.116, whole genome shotgun sequence.
ACCESSION   DADXTG010000115 DADXTG010000000
VERSION     DADXTG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.116"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            63..2390
                     /locus_tag="IWI57_002194"
     CDS             63..2390
                     /locus_tag="IWI57_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2450320.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2516..2845
                     /locus_tag="IWI57_002195"
     CDS             2516..2845
                     /locus_tag="IWI57_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBC2450321.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2885..3889
                     /locus_tag="IWI57_002196"
     CDS             2885..3889
                     /locus_tag="IWI57_002196"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBC2450322.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3886..5315
                     /locus_tag="IWI57_002197"
                     /pseudo
     CDS             3886..5315
                     /locus_tag="IWI57_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5521..6693
                     /locus_tag="IWI57_002198"
     CDS             5521..6693
                     /locus_tag="IWI57_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2450323.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6855..7310)
                     /locus_tag="IWI57_002199"
     CDS             complement(6855..7310)
                     /locus_tag="IWI57_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450324.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2572 a         1433 c         1513 g         2080 t
ORIGIN      
        1 taaaaattaa atttagaata acgtgtaaag gattatttga tgatttttta ttgagaggag
       61 ggatgaaaat gattaagaaa actcaaaata aaatccaacg tatatgtcaa aaacattttg
      121 tttctagact caaagctgta tctaaaagaa aatacttaca tcccattatg atttcgatca
      181 tcttgatttt tactttctta gggagcttat caagtattta tgtaaacgtt tatacagaaa
      241 cgtcaaattt tgctaaccaa acgacagagg agacaaatag tccaccctat tctgaaatac
      301 agtccataga aagcaccaat cagtctagca acaacgagac aacctcaacc acatcctcga
      361 ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc aaaagcagca ggggagaaag
      421 cttattcaga aacaggtaga agccagacat ttagtactgt tgcggccact gcgaatttca
      481 acggacatac cataccagat ggatatacag tgatatatgc caaagatgga gtcattgttg
      541 cggttaacgg atcgatgagt gcggataaaa ctgcgttaca aggcgtaacc aaagcaatcg
      601 cttttattga taaccgtcca gatatccata atctgtttat tggctccaca gtaaatggta
      661 ctactatcac attcccaaaa agtatctata tgggggtggc taccgctgga aatacgatcg
      721 attcagttac acccaatgcg gataaaagtt attcttttac accctcaaca tggggagggc
      781 ttcctgccgg aggaggtatg agtacttttt caatcggtat acagttagca aatgatattc
      841 cattcacggg gtactcgatc aaaaatgggc agatcgttga tggtaaggga aatgtttcaa
      901 attttccaac ctatcaagca gcaacggttc aggtcaatgc gaatcggaca ggaacttttt
      961 cttatacagt caatggtgga gcagcaaaaa tcggaacgta cgggactaca ttatctggaa
     1021 tccatgtggg tgacaaagtg gttatcactc caacagcagt aacgggctat tcttttgcac
     1081 aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga tacggtgacc tatacaggca
     1141 aacaaacacc aatcactatt aagtatcaag actattttgg acagatgatt gctccttcta
     1201 aaataatgaa tcttacttac ggaagtgctg cacaagacct gacgactaat acgcctacta
     1261 tcaacggata tacctttaca gctgtttcag cgacagaaac aaagaaccaa tcagcaatga
     1321 gtgttttagc tagtttagat acaaatggaa atgtaatcgt aaaggacgca aatggaaaac
     1381 agatttctga agtcatccta tattataaga caaatgcaac agtatcaatc aatgcaaatg
     1441 gcagtaagta ctatgatggt ctttctgttg taccgatcgt taaatatgcc tttaatgata
     1501 aaacagaatc aacatccctt ttaaacaatg atctcagtat tgatcaaatc acatggaatc
     1561 caactgattt caaagcgatg aatgaaaagg gagttgaagt aactgcgcct actgaaattg
     1621 gaacctatac gtcttggcaa ttgacaaaaa gcggactagc taaactagct gccagaacaa
     1681 actatttatt tactatcgtg cagacatctg atgtctatac gattaagcaa atcagcggtg
     1741 cggtagagct cagtggcagt aagacatatg atggaaaagc aggtgtcccg tcaatccatg
     1801 tcaagctagc agagggtgtc actagttcgc ttactccagt acaagtggca cttagcagta
     1861 tggattacac agcagatgca caatcagcaa aaaatatggt taatgctggc agctatacaa
     1921 ttaaactaac gaatagcgga atagacaaag tcaaagcagc aaatcccaag ttgtcattta
     1981 ctgatatagc caacacatca ggaacttata ccatctataa agctaacgca gtgattactg
     2041 tcgatgacgc tgcctttaat tatgatgccc aaagtcactc gatttcggtt ggaaatctcc
     2101 acgttaccgg tgttgtctta ggcgaatcat tggattatac cctaacagtt aacagccgga
     2161 cagatattgg atcacaaata gtaacgatta gtttaggcga aggaagtgta aacaataatt
     2221 atacgattat cgcaacagat accgctaagt tgacgatcaa tccgactctc ttgcttcctt
     2281 cgacaggtgg gataggtcta ctgccctttg tctttttagg actgatcttt attttttctg
     2341 gcttctttta ttttatccat cgtaagaaaa aagcaggtga acagcgatga aaaaaacaaa
     2401 aaatgtacgt gtggatatat ttgctgttct cttttttatc ctactggtct tttttatagg
     2461 ttctccgact gtgtcagcta gagaatcacc tattacattt actttgcata atcgaatgtt
     2521 cgaaagcgat aagatgccaa gtatcagtca aaacgacgga ataaatatcc cggataactt
     2581 tcaaggagta aaagggttga acggcgttac tttcgctata tatgatgtct cggatgaatt
     2641 ttacaagctg cggtcagaag gaagtagtgt ggaagacgca caaagaaaac tagcacaaaa
     2701 aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa acagtagacg aggaagaggg
     2761 cattgcttct tttagtgcat cggacaaaga tgagcaaggt cgtgacgctg tttaccgatt
     2821 tgcggaaata aagacttcag attaagtaaa agaaaaatca gcaccatttg tcgttgtgct
     2881 tcctgtgaca gattcatcta atcataaatt gacgaccatc cacttgtatc caaagagcga
     2941 acaaaaaata cctgctgcac tcacattaac aaaaacagta gagaataagc agacagattt
     3001 tgcagatggg gataaagttc cttatctcat caccgtaaca attcctgaga acatcaacga
     3061 gataggaacc tatacgatca aagatacagc tgatcctcag ttatggctag aaccagaaac
     3121 gatcgatttt ttgattggtg gagacaagat tcatacattt cacacacaaa aaacaaaaca
     3181 tggttttgtc ttatcagatt ctggaaaaga tcttagttcg tttgctggga aaaaattaac
     3241 gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa acgacatttg ataacgatgt
     3301 acgtttgacg actgacaatc aaacagtaga aacacatctt gccatccaaa caggaggcaa
     3361 acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta gcagatgctc aatttctagt
     3421 caaaaaaaat gaccaatact tagcgcaaga aaacgggata aatcattggg tagccaaaga
     3481 tgattcaaga gcaacgattt ttacttccag taaagatgga acattttctg tccatggatt
     3541 gtcttttgga agttacgaac tagtggagat taaaccgcct aaaggatata tattgagtca
     3601 atcaaccatt ctctttgaag ttgaaaaagg cagttatgat ccgcttcatc ctataccatt
     3661 ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat ggaaaaacgc cagttcagat
     3721 gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc tttccaaaaa caagcgaaga
     3781 gatgcttgga ggtttctcaa ttttagggtt gttgctagtc ctttcaacag ggacagcttg
     3841 gttttataaa aaacaacaaa aaggaaacaa taggagggaa ataaaatgaa aaacatcaaa
     3901 acaaaaatat ggacgttttt aggaactgct atcatgctgc ttcctttcgt tctcggccta
     3961 ggaactgcag aggtttctag gccgtatcac ctacaccaga aaatgttacg gttaatttgc
     4021 ataaattgaa attcacttca gcaccagaaa atcaaataaa caacggaacg gaattgacat
     4081 ttccgaatag cgaaccgttg aacggcgttg aattcaacgt atatgacatc actgctacgt
     4141 actatccaag taaagacaca gcagttccag cagacgctac acccttcgca agtgtaacga
     4201 cttctggtga aggattagct aacctgactc ttccaggtaa atctgatggg aaagatgctg
     4261 tatacgtctt tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat atcgtgttaa
     4321 gcttaccctt ttataacccg aattcatcag accctgacaa acctttagac acgattcacc
     4381 tatatgctaa aaatgtcatc aaagaaagca atatcaaaat ccagaaagct gggaactttt
     4441 caggggtaga taagttagca ggggcgcaat tccagatcaa aaattcagag aatcaattcg
     4501 tgaccggttt tgatgaaaac actggtgtcg ctctctatga tcctgctgag tcagcgttga
     4561 cattcacaac tgatactgaa ggatccatca gtgtcaatgg attaatagac ggcaattata
     4621 ctttagttga aacaaaagca ccagatggtt atcaattaac caatccacaa aaagaaacta
     4681 cttttatagt aaaaaacgga aaattgcaac aaacaaccgg tatcatccat ggcgcagatg
     4741 aagccaatct tgggaatcca aacggtgtag tcaatcggga aaatacaact acggataaca
     4801 cgattactgt agaaaatgtc caaaactacg gtgatttcca cttcatcaaa caagatgtga
     4861 acacgcaaga aaaattagcc gatgcagaat tttctattct aagagcaaaa gatccaactg
     4921 ttaaattaat gaaaaaaata gcaaatcttg cctcaggaga atacccatat gcttggtcag
     4981 atgaagtcac aggttctgac tgggaagaag tgaaattgat ttctgattca gaagggaaat
     5041 tttcaatcga agggctgaaa caaggggcat actgccttca agaaacaaaa gcaccagaag
     5101 gctacgcatt acctaagaat gaagctgcgt acactgaatt tacagtagat gatgatccaa
     5161 caacttctga tgaaacaacg atcgataacc aacctaaagg aatcttgcct tcaactggtg
     5221 gtatggggat catcgtattc atcttggtag gtactgcttt agttggagga gcagtgatct
     5281 acttcaaaaa acgccatcaa gaaacagaag cttaaactat tggaaacaaa aatcatacga
     5341 taaataaacg atagtgattc aaatggggag aaaggttgtg acaagtggtc ggtctcttgg
     5401 tattcgctca acaaacggca aaataggagg aactattttg ccgtttgttg agcgaatact
     5461 caggtcaagg cgctgatttc acaacctctt catatacata ttagaaaata ggtggacatg
     5521 atgaataaaa gcagaataag aatactggac atcttcatgg caatcatttt agtcgttggg
     5581 atcgggatct tttcttatcc ttttgtagaa gattcactga atgattttct tgcccagcaa
     5641 atgatcatcc attatcaaaa gcaagcatcg aataagaatt cagaggaaat aaaaaaacag
     5701 caggaaaaaa tgacaaagaa aaaccaacag ttggcagagc aaaacgtatc accagggata
     5761 ggcagtttca accaagcagt tgatgcaaaa gcattaaaag atttacctag taatgccttt
     5821 tttatggaac atatgcttgg agtcatcgaa atcccaaaaa tcaatgtcag cttacctata
     5881 ttcgatcaga caacggaaat atttttgcaa aaaggaacgt cattattgga agaaagttct
     5941 tatccgactg gcgggaaaaa cacccatgcc gttctttctg ggcatcgtgg actcccagaa
     6001 gcaaaattat ttacagattt gcctaagttg aaaaaaggag accagttttt catccagatc
     6061 aatggaaaga cattggcgta tcaagtagaa aaaatccaag tggtcttacc tgatgaagta
     6121 gattctttgg gtatccaaaa aggcagagat ctcgtgactt tgcttacctg tactccttat
     6181 atggtcaata cacatcgact gcttgtgacg gggcatcgta ttccttatca agcaaaagag
     6241 gcaaaaaaag cgatccaagg aattgatcaa tggaaaaaat ggaaattttt tatttggttt
     6301 atcggtatac tacttggatc aatcggctta gtttggctac tcatcgctta tttagattct
     6361 ttggccatcg ctaagcgaaa ctatccttta tctttttatg taaagaacac aaacggacga
     6421 ccaatcgaag gaatggtctt ttctgtaaaa acgctcaacg gcaaacacta tattactaga
     6481 gaaaaagttc catttgtgaa agcttctgat gaatacggac tagtgagatt ttctgattta
     6541 aaaggcagga actatcgctt gcaacatgag gagctcctgc ttaaaatcca tgtaaaacat
     6601 aaacattcga aacagttttc aatgaaattg aaaaaaggaa gatataaact gcgaaaagaa
     6661 aaagaggtgt actatttgat tgagaaagaa tgaatgaaga tgtccaaaaa agcagaggga
     6721 acagactaaa tgaaaaaaga gagaagctga cgaaatggac gtgtaaactg ctatttttgg
     6781 tttactatct ggaacgccag cttctatttt tttatgtttc agaagaattt tattattata
     6841 gtataacttt tgaattagtt tccataacat ttggagcaag gggttaaacc acgagcttga
     6901 gcttcgctta aagtggcagg ggtatacgta ccatttccac atttatgtgt atggtatttt
     6961 gaaccggtcg gtgtgacata gacagtttgt tcattatttt gctcaggagc tgcggcttgt
     7021 gcctgctgag aggcctgttc agctgctgct tgctgtcgcg cctgttctgc ttgttgctgt
     7081 gcgattgctt cttgttgcgc ttgttctgct tgttgtcgtg caatttcttc ttgctttttc
     7141 cgttcttctt ctgcttcttt tgctttgatt gtggaatcaa ttgcagcaag acggcttgtc
     7201 aactcttgtt tgttttcagg tagcgcttgg atagctgctg aagcagttgc atagttttct
     7261 tttgtaggat ttgcttcagc ttgtgacaaa gcagattccg cttctgtcac ttttttttct
     7321 agtgcagctt tttccgcttt ttgtttctct tcttcttttt tcttttcaat ttgttcttgt
     7381 tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga agcctgagct
     7441 gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa acttcctgcg
     7501 accaaaaaca atgcaactaa ttttttcact aaatttcctc cttaagtaaa aaacaaccgt
     7561 tttcaataaa tgtccttttc aattgtttac ttacttgt
//
LOCUS       DADXTG010000116         7587 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.117, whole genome shotgun sequence.
ACCESSION   DADXTG010000116 DADXTG010000000
VERSION     DADXTG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.117"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            198..2216
                     /locus_tag="IWI57_002200"
     CDS             198..2216
                     /locus_tag="IWI57_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBC2450325.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2276..2992)
                     /locus_tag="IWI57_002201"
     CDS             complement(2276..2992)
                     /locus_tag="IWI57_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBC2450326.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3219..3761)
                     /locus_tag="IWI57_002202"
     CDS             complement(3219..3761)
                     /locus_tag="IWI57_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBC2450327.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3766..3993)
                     /locus_tag="IWI57_002203"
     CDS             complement(3766..3993)
                     /locus_tag="IWI57_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBC2450328.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(4072..5952)
                     /locus_tag="IWI57_002204"
     CDS             complement(4072..5952)
                     /locus_tag="IWI57_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBC2450329.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6141..6755)
                     /locus_tag="IWI57_002205"
     CDS             complement(6141..6755)
                     /locus_tag="IWI57_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2450330.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6897..7457
                     /locus_tag="IWI57_002206"
     CDS             6897..7457
                     /locus_tag="IWI57_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2450331.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2317 a         1505 c         1252 g         2513 t
ORIGIN      
        1 tagaaatgct atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt
       61 tgattgtcac tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta
      121 tgaaacgtga atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta
      181 tttgatattt attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc
      241 gtttcattca tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc
      301 gttggaaaaa aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat
      361 ttttttactg ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag
      421 aaactatcca ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa
      481 tcatcattat tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg
      541 ccttgactgc taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg
      601 aaatgcggga agagcctgtc tttccttcag atttaaaaat gctgaccgga ttgtcagaac
      661 tattatcgat ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat
      721 tgattactag tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt
      781 ggaaacgtcg atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca
      841 ttaatcacaa aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt
      901 tctttaacca gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata
      961 tcgacattaa aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa
     1021 tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca
     1081 atcagacgat cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc
     1141 tgacagtacc tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag
     1201 gattgatgtt atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga
     1261 caggcttgag tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg
     1321 agcgtcagaa aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc
     1381 cttttacagc tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt
     1441 tctattattt agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac
     1501 gcatatcgga tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacggaaa
     1561 cgtcacaatt catccaatta tccactatgc aaaaccatat gccttatgat gattactata
     1621 gtgaattgaa ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac
     1681 aaacttatat gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa
     1741 tcgacaaaat cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct
     1801 atagtgggaa tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt
     1861 ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta
     1921 ataactttgg tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt
     1981 tgttgacaga agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt
     2041 ctaatcgttt taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa
     2101 aaaatttaac tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc
     2161 ttgttgcagg taaccagtac agtgcaacat gggcgacaca aaaaataaaa gactaacaaa
     2221 taaactgagc aaggggttgt gataaaagac atgtcacaac cccttgttta tctcattaat
     2281 ttgcttgtcc ataaacgatt tgattaaagc catttttcaa ttcattattc gctgtctcgt
     2341 tgaagtaaag ataattatcg ggattcccct gattttgttg tgtcaccaat acttttggct
     2401 gattttgctg aaacccgaat agatagacgt gctttttcag atatggttct gtctctgcat
     2461 cggaatagac ggctgtgatt tgatagtctg cttgacctgt cccactttct gaccattctg
     2521 cagaaacagg cactccacca atagccatct tccactcccc gttcaaaata gcttgaggga
     2581 ctttaagacc atataagtcc acactcattt gattcgtata acttttgtat tgttgtccca
     2641 ttgtttggcc ccatttggtc ataaatgcct ctaattgtgc ggatttgtct gcattccaca
     2701 gtgtttgttc aacttgttcg ctgctttctg aagaggaaga tgaagcctct acagaagaac
     2761 ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat
     2821 ctgctgtttc tgtacttgct gcagttgact tgctctctgt gctagttgtt gtagtagttg
     2881 caactgttgc tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac
     2941 aaccagctaa caaaaaagct agaccaattg ttactaaaat cctttttttc aattaaaaaa
     3001 ctcctttcga ttactcccac ttctttattc tatcaaataa gcaatgtgat tttattgatt
     3061 tttcgtattt tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt
     3121 tgtctgtaat acatcagcgg gtgcttcttt tattttaatc ttcgtgattt tccaatttac
     3181 ctcacgcttt atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca
     3241 tccaaatctt gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta
     3301 ttgaaactta aaagttcaac aagcaattgt tgcaagccat atttttcttc tttttcagct
     3361 aatttgattg cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc
     3421 aacattgttg aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc
     3481 atgacttctt ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt
     3541 ttattgaacc atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc
     3601 gaagatgtac ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa
     3661 tttgctaata catgccccac catggcgcca gcagttggtt tatgatggtc gatatctgct
     3721 tgttttgttt cttgaacaaa tttttcttcc actatattca ttgatttacg cctcttttac
     3781 ttttgctgat tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg
     3841 gacttcatct ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt
     3901 gcttacccca ggctgttgag aaaccgcatg ttcgattttt tgcatacagc ttggacaagc
     3961 tagtgtatct aattgcataa taattttttt catcgtagtt cttccttcct ttttcttgat
     4021 agtcttattc tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt
     4081 ttcttctttg ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa
     4141 gatactagct tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc
     4201 gttgagtaaa aataagacga caccaatggc aatcactaca ttttcacggg tatttaaaac
     4261 agttttctta gccaagcggt aggcatgaac caacgattca aaattagatt gcatcaagac
     4321 aacgtcactc gtttcgatag caacatccgt tcctgatccc atagcaatcc cgatatcagc
     4381 cgcagccaaa gagggagaat cattgattcc atctccaata aaagcaacgc gcaatccttg
     4441 ttcttggtat tttttcacaa attgaacctt ttcgtctggt aataattcag catggacttc
     4501 atcaatgcct aacatatcag ctacataatc agcagtcatc tgattatcac ctgtcagcat
     4561 cactaactgt tttgctcctg cttgtttcaa cttagctaat tgttcagcga cttctggtcg
     4621 aatcacatct gacacaccta tgatttgagt gatttttttg tcgattgcta caataatgac
     4681 cgagctgcct gtttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag
     4741 tttgtgagcg gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc
     4801 tgtgattcct tgtcccttga ttgtctgatt atctttgact gctaattgtt gaaaatcaat
     4861 gtctttctcc tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc
     4921 taatcgagcc actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga
     4981 tacagcagta gttcctttgg tcaatgtacc agttttatca aagacaaaag tatcgatttt
     5041 ggaaaaagta tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc
     5101 gattcctgct acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac
     5161 caaaacagtg attgctaaac gaaaatcacg actaatcaga ccaacaacca acgcaataag
     5221 taaaacggtt ggagtataat actgagaaaa tcgatcaatg aatttttccg catgggattt
     5281 ggtatcttgt gcttcttcta ctaattcgat aattttggca aaggtcgtat catctccgac
     5341 cttagttgct tcaatcgtca cgtagccatc tgctaaaatc gttccagaga agacttcatc
     5401 tccactgatt ttgacgactt gtcgtgattc accagtcact gcggcttcat ccgcataccc
     5461 tttcccgtca atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat
     5521 atcgccttct tccacgtcat cgatttccac ttcttcagtc gttccatctt cttggacaac
     5581 actagcagta gatggggcca tctcggtcaa agccttgatt gactcccgcg ttttggtcaa
     5641 tgttttttgt tccagatagc taccaaacaa aaacaagaat gtaacgatag ctgactcctt
     5701 gtattctccg atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat
     5761 aactttgact ttcactgcct gatacgcatg gatcataata ggtatgaccg caataataga
     5821 agctatggct agagcaatgt tatagaacaa gacaaaacca gtaaattttc cgataaaacc
     5881 aaaaataatc aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt
     5941 ttgtaatttc atctttctcc ctcttttcct ttcattctta cgagactaag tatagatact
     6001 tatccatcat aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt
     6061 agaatacact acaaaaaata gttagcaaga agactcacta aaagtgatct tgccaactat
     6121 tgtcgtttct atcgaaattt tcattgaatg attttcctgt acagcataca tcggagatct
     6181 atttctttat ctaagatttt tatcttgcta attttttcct ttagtctttc aaatccacgt
     6241 cgttcataga attgatacgt acagccacta tctgtaaaaa ggaatatttc ttttcctttc
     6301 tctctttttt caaattctgt tagcaacttg gtaccgatgc cttttgtttt caaatcagga
     6361 tttgcagcta aaaaaatgat ctctccatct ggcgaatgct tctttaaata tgctgcaagc
     6421 atttgttcat ttgctgtctc gtaagctcct ttactttctt tagcaaatat attttgtaaa
     6481 aaatcgaata tcttaacata tatttgctgt ccaaaagaac gatgtttctt tttttctcct
     6541 tttatctccg ccagcaaaac acctgccaac tcgtcctctg catacaaagc aagaatttga
     6601 gttgctcgat tcaactccat atacaaaaaa tatcttccgt aaagattcag cagaagttca
     6661 ttttccatat atctctcaaa gttcattcct tcaacagcaa aatagatagc ttttttgtaa
     6721 tctttctttt ctaagggttt aaaagttagt ttcatcattt cgcctcctca acttcaatga
     6781 ttgtgttata cttacattat aatagacaca gtgtctacta tctataatca atcatctata
     6841 agtttgtcga ttaagcgaca gctttgcttt gatatgtcgg ataaggagat aggtaaatga
     6901 aaaagacaga tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat
     6961 tattagtaaa taaaaagatt caagcaatca ctatacgcga actaacagaa acggcagatg
     7021 tacatcgttc gactttttat acacattata aggatatcta tgatctatat gatcaattag
     7081 aaagcaactt ttttcgggat ctaaatgaaa ttctctctta tgatccagcc catagctatg
     7141 aggaactata cactcgcttg attgactatg tttacgcaaa tactttttta tatcgcttat
     7201 ttatcctcaa ccatggtgat atttcgtttc agaagaaagt taaacaagtt attgaagaaa
     7261 actatctgaa catatggcta tatgaagaca agaaaacgat tatcacagaa gaaatgagat
     7321 tctttactac gtaccacgtc caaggttgtc tatccgttct caatcggtgg gtagaaagca
     7381 attacaagta ctccaaaaaa gaattattag aactcttaca gaaactgaat gatcacatag
     7441 agaaaatcat gccttgaata aaaacatact aagcttagtt aagaagaacc gtaaccttct
     7501 cgtataacaa ctgaatctta tttagccaaa ttgtttactg aagatttttt gagtaaaatt
     7561 tctataattc ctacttattg aaaagta
//
LOCUS       DADXTG010000117         7302 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.118, whole genome shotgun sequence.
ACCESSION   DADXTG010000117 DADXTG010000000
VERSION     DADXTG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.118"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            61..1545
                     /gene="lsa(E)"
                     /locus_tag="IWI57_002207"
                     /gene_synonym="lsaE"
     CDS             61..1545
                     /gene="lsa(E)"
                     /locus_tag="IWI57_002207"
                     /gene_synonym="lsaE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Lsa(E)"
                     /protein_id="HBC2450332.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            1599..2402
                     /gene="lnu(B)"
                     /locus_tag="IWI57_002208"
     CDS             1599..2402
                     /gene="lnu(B)"
                     /locus_tag="IWI57_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HBC2450333.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            3090..3911
                     /locus_tag="IWI57_002209"
                     /pseudo
     CDS             3090..3911
                     /locus_tag="IWI57_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            4054..4278
                     /locus_tag="IWI57_002210"
     CDS             4054..4278
                     /locus_tag="IWI57_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450334.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            4293..5162
                     /locus_tag="IWI57_002211"
     CDS             4293..5162
                     /locus_tag="IWI57_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBC2450335.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5143..5877
                     /locus_tag="IWI57_002212"
     CDS             5143..5877
                     /locus_tag="IWI57_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2450336.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            5910..6818
                     /locus_tag="IWI57_002213"
     CDS             5910..6818
                     /locus_tag="IWI57_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBC2450337.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            6815..7063
                     /locus_tag="IWI57_002214"
     CDS             6815..7063
                     /locus_tag="IWI57_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HBC2450338.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(7066..>7302)
                     /locus_tag="IWI57_002215"
     CDS             complement(7066..>7302)
                     /locus_tag="IWI57_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBC2450339.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         2526 a         1060 c         1513 g         2203 t
ORIGIN      
        1 ttaatgccga aaaccgactt tgcttataaa atttttataa tgtaaaggag gaagaaatat
       61 atgtccttaa taaatgtttc aaatctaact ttttcatatg aaggaagtta tgacaatatt
      121 tttgaaaatg taagttttca gatagataca gattggaaac tcggttttat tggaagaaac
      181 ggacgcggta aaactacttt cttaaattta ctgcttggca aatatgcgta ttccggcaat
      241 ataagttcta cagttaagtt tgagtatttt ccttatgatg tggaagataa gagtctatat
      301 acaattgaag taatgaagag tatttgtacg gaatttatgg attgggagat ttttcgtgaa
      361 atatcattgc ttgatgttca agaagatgct ttatatcgtc cgtttaatac attgtcaaat
      421 ggtgagcaaa cgaaggtcct tcttgcagct ttattcctta cagcgagttg tttcctgctt
      481 attgatgaac ctacaaacca tcttgacatc gatgcacgta atgtagtgca aaactatttg
      541 aaacgcaaga aggggtttat tttggtatct catgatagaa gcttacttga tcaatgtgtt
      601 gaccatatac tatctatcaa taaaacgaat atcgaaatcc aaaagggaaa ttttacttct
      661 tggtgggaga acaaaacgtt acaagataat tttgaactgg cagaaaacaa gaaactcctt
      721 aaagaaatag gaaggttgtc ttatgcagca aaacgtagtt caaactggtc aaataaagta
      781 gaaaaaagta aatatggaac aacaaattct ggttcaaaac tggataaggg ttatgttgga
      841 cataaggctg caaaagcgat gaaacgtgcc aaaaatattg agtcaagaca tcaggaagcc
      901 gttttacaaa aatcagaact gctccacaac attgaacaat atgatgactt aaaaatttca
      961 ccacttgaat ttcacaaaga gtgcttaata gaagcgaatg atttatcatt gtcttatgga
     1021 gataaagaag tatgcagtaa tcttaatttc agagtcaata ttggtgatag agttgccatt
     1081 atcggaaaaa atgggagtgg taagtctagt atcctaaaat tgattaatgg agatgatatt
     1141 aaatttaccg gaaattttat gctagcaagt ggactaaaaa tttcttatat ttcgcaagat
     1201 acttcatatt taaaaggtaa tctatctgaa tttgcctata ataataagat cgatgaaact
     1261 ctatttaaaa cgattcttcg taaactggat tttaatagag agcagtttga taagaacatg
     1321 gtggatttta gtgctggtca gaaaaagaaa gtactaattg ctaaaagcct ttgtgaaagt
     1381 gcacatttgt atatatggga tgagccattg aactatattg atattttttc acgtatccaa
     1441 attgaaaaaa tgattttgga atattgtcct acactattgt ttgtggagca tgatgatgct
     1501 ttttgcaata acatttgtac gaaaaatatt aatttaggtt tgtagagatt ttgagttgcc
     1561 acaataacta aaagatattt acatgaaagg gtgaagaaat gttaaaacaa aaagaattaa
     1621 ttgcaaacgt taagaatctt actgagtcag atgaacgaat tacagcttgt atgatgtatg
     1681 gatcgtttac caaaggagaa ggtgaccaat actctgatat agagttctat atatttttga
     1741 aagatagtat aacctcgaac tttgattcat ccaactggtt gtttgacgta gctccgtact
     1801 tgatgcttta taaaaatgag tacggaacag aggtagttat ttttgataat cttatacgtg
     1861 gggaatttca tttcctttct gaaaaagata tgaacataat cccctcgttt aaagattcag
     1921 gttatattcc tgatacgaag gctatgctta tttacgatga aacagggcaa ttagaaaatt
     1981 atttatcaga gataagtggt gcaagaccaa atagacttac tgaagaaaat gctaattttt
     2041 tgttgtgtaa tttctctaat ctatggttga tgggaatcaa cgttctaaaa agaggagaat
     2101 atgctcgttc attagaactc ttatcacaac ttcaaaaaaa tacactacaa cttatacgta
     2161 tggcagaaaa aaatgctgat aattggctaa acatgagtaa aaaccttgaa aaagaaatta
     2221 gccttgaaaa ttataaaaaa tttgcaaaga ccactgctcg attagataag gtagaattat
     2281 ttgaagccta taaaaattct ttgctattag ttatggattt gcaaagtcac cttattgaac
     2341 aatacaactt aaaagttaca catgacattt tagaaagatt gttgaattac attagtgaat
     2401 aggagagtaa cgttattcac agtattgggt actgaaaatt aacagcatac ctcagttttt
     2461 caaaaaacag cgaagcctat ggatatgttc ccgtctaccg atagtgcaaa aagtgtcgtg
     2521 gatatgtttc cgagaacgga catacacaaa tgacattcat tctgtcctct caatccatgt
     2581 ggaagcagta tgtctgttaa caagaaaata attattgttc gtcctgacga gttgttagac
     2641 tcgaaaagga taaacatcat ttttagaacc ggtagccgat ttttggctag cggttttttg
     2701 ggctctttgg caaatagtgg tgattgaatc tcgcgctatc ctatggaaaa ccgccttgtg
     2761 tgaggggctg cgagaccgct tcgatcctag aattgctgaa gcacgaatct agcgtcactc
     2821 acatttcatc ttttgacgta gctgagacaa ggtgtttttc ttcgatccgc tagtggttaa
     2881 tctctttttg gtgcaaatat ggtttagaat ttctttgcat ggcttaaggg agtggagaat
     2941 cggatattag taggatttac atccacagat aaaaataggc ttgattttaa cagcgaaact
     3001 aaaataccct acttaaatga agtgatagat tagatgttgg gttgttagga gatgcttaaa
     3061 aatcctatgc aatcaatagt ttttagtgtt tgttgacttg atccctaaat cgcctgagta
     3121 ctaaacgttg agtgtgtctg cttactgtca ctagccagta agtgagactg tagttaatct
     3181 ctcattttcc ccagtattat gttgaaaata gccatgaagc gattatttct aaagagttat
     3241 ttttgcaggc tcaagaagaa atttatcgga gaagtaatat ctacacagga gaaggcaaga
     3301 acaaaagaat ttatagtagc aagtacgctt taagtgccat caccttctgt ggatattgtg
     3361 gcgatattta taggagaacc tattggaata ttcatggcag aaaagaattt gtctggcgat
     3421 gcgtgactag aatcgagcag ggtcccgagg tatgtaagaa ccgaaccgta aaagaagatg
     3481 aactctatgg tgcggtaatg accgcaatta ataagctact tgctggtggc aacaatatga
     3541 taaagacact ggaagaaaat attcatgctg tgattggaga aaccacagaa taccaaattt
     3601 cagagattaa taccctacta gatgaaaaac aaaaagaact aatcaagctg gccaataaag
     3661 gtcaagatta tgaatacctg gtagatgaga tcgatgagat gcgagataag cgtcagactc
     3721 ttttagtaga agatgcctcc ctcagtggcg agaacgagcg catcaatgag ttgattgaat
     3781 ttatccgcaa gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa
     3841 tccagaacgt taaggtctat gaagaccact tcgtcatagc cttcaaacca ggtatcgaaa
     3901 tggaaatatg aaaaccagaa gtaaatagcc catgactctg aagtagagtt gtgggttttc
     3961 ttttaattgc tgattataat aatattttaa tgactattgc atttatcaac caaatggttt
     4021 atgttattct tagcataaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa
     4081 ctgtgtgagc aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca
     4141 cagaatttta ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt
     4201 gctgatgtgc taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata
     4261 aaaatatcta acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa
     4321 cagagaaatt atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa
     4381 gaagcaccca tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat
     4441 ttgacctgac agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc
     4501 ttgttgtacc tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa
     4561 acgggtgtca tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag
     4621 atacggagca aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa
     4681 gtgctatgta tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct
     4741 tatgcgaatt aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga
     4801 acttttttat atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag
     4861 aggataaata ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac
     4921 tatttgcatg taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa
     4981 cttttgaata taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg
     5041 gtctttctct ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa
     5101 ttgtatcgga gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat
     5161 gatgataata tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct
     5221 ggtgcgggag aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg
     5281 cttgatttag gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc
     5341 tctgtagtag gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat
     5401 tttccacaga ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc
     5461 tttgatgtaa tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata
     5521 aaaaagatat ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct
     5581 gtttttactg ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat
     5641 ttcccggtgg acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag
     5701 gttacaaaat atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt
     5761 ataataaatc agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg
     5821 gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa
     5881 tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag
     5941 tactttcttt agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg
     6001 caaatattaa tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg
     6061 atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga
     6121 tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta
     6181 tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata
     6241 attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa
     6301 gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg
     6361 atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta
     6421 aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa
     6481 tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata
     6541 agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga
     6601 tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg
     6661 tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa
     6721 caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca
     6781 catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca
     6841 cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag
     6901 gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag
     6961 ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa
     7021 tactgataag ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga
     7081 atatctcaaa gttcctccaa aacgtcgttg tgctcgactt aatagaacac tgtaagccag
     7141 taatatgctt tcaaatctac tctctagccc tttaacagaa cgtgagttga aattttgaca
     7201 acctaatttt tctaaggaag aaaaaacagt ctcaactgtt tttctttgtt ttccaagtaa
     7261 gctgtggtct agtttttctg atttatccat gtttttacgt gg
//
LOCUS       DADXTG010000118         7223 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.119, whole genome shotgun sequence.
ACCESSION   DADXTG010000118 DADXTG010000000
VERSION     DADXTG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.119"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(48..305)
                     /locus_tag="IWI57_002216"
     CDS             complement(48..305)
                     /locus_tag="IWI57_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBC2450340.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(298..567)
                     /locus_tag="IWI57_002217"
     CDS             complement(298..567)
                     /locus_tag="IWI57_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBC2450341.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(698..877)
                     /locus_tag="IWI57_002218"
     CDS             complement(698..877)
                     /locus_tag="IWI57_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450342.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(1258..1965)
                     /locus_tag="IWI57_002219"
     CDS             complement(1258..1965)
                     /locus_tag="IWI57_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450343.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1958..2395)
                     /locus_tag="IWI57_002220"
     CDS             complement(1958..2395)
                     /locus_tag="IWI57_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450344.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(2410..2661)
                     /locus_tag="IWI57_002221"
     CDS             complement(2410..2661)
                     /locus_tag="IWI57_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450345.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2661..2867)
                     /locus_tag="IWI57_002222"
     CDS             complement(2661..2867)
                     /locus_tag="IWI57_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBC2450346.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(3304..3564)
                     /locus_tag="IWI57_002223"
     CDS             complement(3304..3564)
                     /locus_tag="IWI57_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450347.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3639..3842
                     /locus_tag="IWI57_002224"
     CDS             3639..3842
                     /locus_tag="IWI57_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBC2450348.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3983..4468)
                     /gene="ssb"
                     /locus_tag="IWI57_002225"
     CDS             complement(3983..4468)
                     /gene="ssb"
                     /locus_tag="IWI57_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBC2450349.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(4471..5370)
                     /locus_tag="IWI57_002226"
     CDS             complement(4471..5370)
                     /locus_tag="IWI57_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBC2450350.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(5383..5976)
                     /locus_tag="IWI57_002227"
     CDS             complement(5383..5976)
                     /locus_tag="IWI57_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBC2450351.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(6052..6201)
                     /locus_tag="IWI57_002228"
     CDS             complement(6052..6201)
                     /locus_tag="IWI57_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450352.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(6217..>7223)
                     /locus_tag="IWI57_002229"
     CDS             complement(6217..>7223)
                     /locus_tag="IWI57_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBC2450353.1"
                     /translation="YEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGW
                     KKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAG
                     KTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVE
                     KEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGST
                     LQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTAT
                     QLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK
                     "
BASE COUNT         1878 a         1462 c         1178 g         2705 t
ORIGIN      
        1 ggatcaaaaa cttttaagtt tctgaccctt tccttacttc cgattggtta atagtgatct
       61 tttgcagaat aaataaatat cgtttcattt tcaactctat atatcagtct atgttcatct
      121 gtaattcttc tggaccattt tccagataaa tcatgcttta atggctcagg ttttcctaat
      181 ccagcaaagg gggaacgatc gatatctttt attaacttgt taatcttttt tatattgctt
      241 ttgtttcctt gctcatgcca ataaagataa tcatcccaag catcatcaga ccaagcctta
      301 atcatcagat tcaacctcga ttaagtcatg tgttttaaat gcacctttgg agaattgttc
      361 atctcctcga cgaatttttt ccatgacgta attattagaa agtgttctca acgtttcttg
      421 catagaatca taatctcttt ttgataatac aacaactgta tcttctacat ctttacttgt
      481 tacaataagt gtttcagcat cctcattaac ttgtttcata taactacgta aattttggcg
      541 gaaatttgaa taagctactg cttccattcc tttcacccct tatttatttc tataaaacaa
      601 ttgtacatta tattgtacaa ttaagcaaat gattaatatt ctaacttctt ataattaaaa
      661 gtgctatcac ttttagtttt taggataaaa agtgacgtca ctttttgagt gctggtacct
      721 caaatcctgc tttttcttgg gcaatcaata aatctaacgc aatacacata atcctggatt
      781 cacttttgcc gtacttctgc tttagttgct gcaattcttt ttgtctcttt tcatccaacg
      841 tcacgggtaa ccgtttattt cctattgcaa ttggcattag tactctctcc tttcatcgaa
      901 ataaccctat taaaaatatg gctgcaatca acttgaatat tagactcaca agggttaaca
      961 caaagcgcca aaagatcaaa caaaatacca gtattcctaa gaatactacg aagtttaaat
     1021 gtagagctgc accatgacgc ataagtatct cgccaacaaa cagtacgaac aatagaggaa
     1081 tgattttatt catatggatc tccttctttc tcttaggtta tcgggctttg cccgcttatt
     1141 tcgcaccacc taagcggcgg gggcggttgg atcgattggt gcgaaataag aaccattccc
     1201 ttaatttcaa ggttttttgt tcttatttca cacatcctca cttttcggta taaatggtta
     1261 ttgacctttt ttcctttcaa atcgttgata tggcattcgt ttacggtcaa gttcattttg
     1321 gatttcttct gtatcgtcgt aagtgtaggt gtctaaaaaa tcagttaaag tcgtgtacca
     1381 gtcgtcgcct gggttatcta cttcttctac atattccctc aaaaatgctt cgggtacagt
     1441 gagttcataa tccattccca ttaacacgat agattccgtc ccacacgtca aaaatctagt
     1501 aaatacaagg taataatcgt ctaccgtcgc atctttgctg tttagctctt ccatttcttc
     1561 ttccattaat tccatgctct caaaaattga ataatattct gattctgagt aatagtgttt
     1621 cctgcaaatc gtgtagctga caaagttttc gaccagctca ctcctttcta ggtaaacatt
     1681 catgatatat ttttctgttt ctagtcgtag aagctccgct ccttttttta atgtgtcagg
     1741 acgacgttta tacacgtcta aacgattatg gctgactgta accttcgttt gactcgtttc
     1801 actttccata tacaactgtt cttccactct ttgaataaag tacttcattt cttccataca
     1861 actaaagtct ttttgaatcc gtctgattac ttcattcaag tgttctttgt ttgttaaaaa
     1921 ttgcgaaaca tcttctgttg ttagctgata aaccacctta ttcattatct ttctcctccg
     1981 attcaatcgt ataaaatgca ccattttttt cgatattttc aaaagtatct agccagtcgc
     2041 tagccattgc tttagctttc ttaatctgtg cgaattttgc ataagtcagt tctaaggctt
     2101 gatattcgga aggtttgata gtgcttggtt tgacacctac ttccaagcat agtaaggctt
     2161 ggaaaaacca ttttccattt tgttttttca gttcttccag tgttcgatag tacgtcgcaa
     2221 tgagattcat ttcgacacct gattcaatca tttcttccac tgcgactgac aatttttcct
     2281 tgtaatgttt tttgggtaca cgtgtttcat ggtataggaa cgtcatatcc tccatttttt
     2341 gtgccatttt atccaaaggt aatttgacaa attcgtcata gtaattttga gccatatttt
     2401 ttctcctttt tagttctcta atttggttaa ccaatattcg atttcataag tttccatgtt
     2461 ttcttgataa tgcagttcat atcctgcaat cttcactagg tcttcaaata gcatagtcgg
     2521 tgcatcctct actaaagggg tttctatcgt ccagtaccgc ggaataagta aagcttctgc
     2581 tactttttca gaaatgactt ctttgtccac caaaatttga acgacttctt ttacagtcaa
     2641 taatttgtgt tgtgttttca tcaccgtttc tttactttct catcaatcaa aacataatcg
     2701 ccataagaca aggtatattt tttcccgcat gttggacatt gatacaaacg atagcttttg
     2761 atacatttcg ctctttctcc acaatcgcat tcgacagtat gtggtgcatg atagtttggg
     2821 aataattcgc tgaagggaac accccaataa tcaaatttct ttcgcatgat tgattctgcc
     2881 tcctcttagg aaaggtttta tttcctttcg ttagttcttt ctttattgat ttgttggcca
     2941 gaatgaatca acgaaaagcg acaaggacaa gggcgcgcag cgtgccattt acccttgtcc
     3001 ttgtcgcttt ttggtgataa aaggcaactt gttgccggtc tggcaaatca ataaagagat
     3061 agcgaacagg aacgaacctt ttggtgagag gaagtttttt ctattttgtc ttgctttgca
     3121 agacaccata tcttagtgaa cgtgcaacgt tcacttcctt tcttctcgaa cggcggtggc
     3181 ggttggagtg agagaaaggt ttttcaattg agaaggcact tgtgtcttcc taaaaaggtc
     3241 ttttcctgtt tctatgtaaa acaaaaagag ctgcaatcct catttaggac tgcagctctt
     3301 cttttatatg cccatcgatc gcgtaaaatg atcggggttt ctaatttgca tcgattcaat
     3361 gactaattga ttccgtttat tgaaatgacc tgtgacggca atattgtatt ttccatcatc
     3421 cataattaac aatttatcga taatttcaat atctgcaact acacaattaa tcgattcatg
     3481 aattgtttgt aaagtaaagc gaaccaataa tggttgtgtt tttattactc ggattttatc
     3541 aagcaaacca acatatttgt tcatcacgat cacctcgcta aaattatagc aaaaacagct
     3601 aaaattgaga atttttattc gattttagat atgctataat gatcaagaca gtaaagtttg
     3661 tgacggtggc tatccgattg aaagtgaggt ggtgcgtatg tgcgtacttc caaaatctta
     3721 cagaaaggag aagccttttg cctgttgaaa cggcgctagg attaatgatt agtttcggtt
     3781 ctttgatcgc aacaatcatt tttggaatct tagcagttgt aaacaacaat caaaaaaaat
     3841 aaccgtcagc tttagccggt cgacgattat ttttcaaaaa taatattaag ctggtcaccg
     3901 tcttaaacgg tgctgttagg ggagttgtta gcgcaactcc tcttttcgta tccattataa
     3961 caactttatt gatcttttac aatcagaatg gtaaatcatc atcactaata tcgattgatt
     4021 gtccgccaaa tggattttgt gaagcgttca atccattgtt gttaggcgta gctacatccc
     4081 gttgttcaaa ggtggtaggg cttggactgc tggacgattg atcagcgtgc gcacggcttt
     4141 ctgtcgctgc tttactttct aatagttgga aattatcggc cacaacttct gtcacataca
     4201 cacgttgtcc ttgttggtta tcgtaggttc gggtttgaat ccgtccaaca acgccgatca
     4261 aactgccctt tttagctaat gtcgccatcg tttctgctgg tttgcgccaa atcacacagt
     4321 taataaaatc agcttctcga ttgccgtttt gattcgtgaa atttcggttg acagctaggg
     4381 taaaagtcgc tactgcagta ccactcgcgg tataacgtaa atctggatct ttggttaagc
     4441 gtcctactaa agttacatta ttgatcatct ttatctctcc atttcttgat tcatttgttg
     4501 tgatctttta aactgccgtt ttctttcttg attttcaatt ttgatcacac ttttcattag
     4561 gtgatcaatt tcgttgactg tatctgtaaa tgaaatggaa ttcgcaaacg tgttaactga
     4621 gcgatatttt tcccactgct tttccaaata gtcatttcct ctgatcttat ccatttctct
     4681 ttcataataa aaggacaacg gttttgtatc cccacgtttt gtaaatgtat ttcgaacact
     4741 tttgtataac tctttcgtat cgatattttc tagtttcgga atggtaaata agtcgtagta
     4801 atcttttgct ctcgaattat ctgtgccaaa gctgaacgta gcacttagct tttctgcaat
     4861 aatttgttct gttggataag ctggaatctc cactttttta tcttcaaaca ttaattggtg
     4921 atagtgtgta tctacttcag gataaataga atctcctgtt gtcacgtcca ttttaaacgg
     4981 tacacgtact ttctctaaat tagccaccaa tttaatttga aatccaggat aaaaatcggc
     5041 ttctcttgtt tccttcattc cttgaataga gaaaacaatg ccttcttttg tgggttttct
     5101 agttaaatca ttaaaaatgc tcttcaattt ttcttcagtt aatttgctat ttctaaatgt
     5161 cgtatcgata tcaattgttg tccttctatc gattccatat tttgatccta ataaaaagcc
     5221 gcctttcaaa atgaagtcat ttttgtactt tgattcagaa atttgttcaa ttagacgatc
     5281 catcataaaa tgccgttgca ctaactgtgc atttaatcct gtactctttg ctaaatttct
     5341 tgtttttgct gcaaattgtg tcggtgtcat ctttttcctc catcaatata agctttgcat
     5401 gtggtatttc atttcttttt ctacatgtag ttttgtcatg taatctctta atttacttgg
     5461 atctctcaat gggtcttcca tgtactcttt taatgcttct aatttcattt catattcaaa
     5521 ttcatatctc ggattccaaa tatcacaaag tgtacgctct ttatcataca tagtaatagg
     5581 atttccttga aaggaagttc cctctacttt tcctagttca taaacttctt tcttcacgaa
     5641 atgaaattgt aactgctgct cttttgtaat tgaacgatca acgttatagc catggggaaa
     5701 agttaagtga atgaatttcg gcatattcgt agaaagctgg taaatatcta aagtcgtttc
     5761 atgagagaag attcccctgg aaaatcgttg ctgaagccaa aataaatcat ctattccatg
     5821 accatcctct acataataac cacgatcaac tttgatcaat tttccttgat tcaccaatct
     5881 ttgtacagtg gttgctgcga ttccaaattt cctggcttcc ttcatagata aatttccatc
     5941 gcgaatagcc aacaattctt tcagtttatc ttgcatttac ttcccctccc ttcgttacaa
     6001 atacactaca tcaaatatat catagtagtt tatttgtaac aagattaacg tctatcgatc
     6061 taattcgatt tctttttgaa tgcgctgctg gttctttaat tgttgcagtc ttcttttatt
     6121 tgcacgtaat ttctcttgtt ctaaacgaat ttttgctttt tgattttcga tttcttgttg
     6181 tttgtgtgtt gtatttcgca taggataacc ccttcattat tttgtagtgc ggcgtgttct
     6241 tttcttatag ttcgtttttt tcttttcaga aaattcaaag cctgttgtaa agtcttcttt
     6301 taaaacgatc gttgcttcaa acgaaccttt ctgacctttt agtcctctga ctgattggct
     6361 tgttcggccg ttttgaatga tctctttcag ttgtgtggct gtgagtttct ttttggcaat
     6421 cgttgtccat aatttaaaat cacaggcttc agacgtacat gccgccacat tttgataaag
     6481 caagacttct tgttcccgac attttggaca aacacctaaa tgacgcgttg cttcctgttt
     6541 ttcactggct gctttcacct cttgtaacgt tgatccaaaa tctgttgact ggatttgtcc
     6601 cggtacgact tccaacaagt gagagacaaa tttttggata ttagctaaga aagtagcttg
     6661 tgttcctttc tgctcaccaa tctttaataa gtaactttcc catttggcgg tcatttcggc
     6721 acttgttagc agcggctctt tttccacggc ttggcaaagt aaaatccctt tatttgtcac
     6781 cacaagctta tttttttcta ccttgatata ttccttttgt ttcaaagttt caataatatt
     6841 tgctcgtgtt gcttctgtac cgattccttc tacttctttc aatatggatt gtgcttcttc
     6901 gctatccacg gtttttccag ctgttttcat cgctgtgatc aacgtacctt cggtataagg
     6961 ttgtggtggc ttcgtttctt tttcaaggac ttgtagatct accgtcaccg cttctccttt
     7021 tgtcacttta ggaagtagcg gttcttcttc cttttctttt ctgtcgctct ctttgccaaa
     7081 cagctttttc caacctagat ctactggtac ttttccgact gtctttagct gaagctgctg
     7141 aacttgcgtc aagatcgttg tttcttcata ggtatacttt tccgcaaaca tggccaccgt
     7201 tgtttttaac acttgttcat aaa
//
LOCUS       DADXTG010000119         7064 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.121, whole genome shotgun sequence.
ACCESSION   DADXTG010000119 DADXTG010000000
VERSION     DADXTG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.121"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            308..574
                     /locus_tag="IWI57_002230"
     CDS             308..574
                     /locus_tag="IWI57_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBC2450354.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            574..2301
                     /gene="ptsP"
                     /locus_tag="IWI57_002231"
     CDS             574..2301
                     /gene="ptsP"
                     /locus_tag="IWI57_002231"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBC2450355.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            2465..3511
                     /locus_tag="IWI57_002232"
     CDS             2465..3511
                     /locus_tag="IWI57_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBC2450356.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            3528..4751
                     /locus_tag="IWI57_002233"
     CDS             3528..4751
                     /locus_tag="IWI57_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBC2450357.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            4840..5727
                     /locus_tag="IWI57_002234"
     CDS             4840..5727
                     /locus_tag="IWI57_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBC2450358.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            5813..6043
                     /locus_tag="IWI57_002235"
     CDS             5813..6043
                     /locus_tag="IWI57_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBC2450359.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(6100..7050)
                     /locus_tag="IWI57_002236"
     CDS             complement(6100..7050)
                     /locus_tag="IWI57_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBC2450360.1"
                     /translation="MKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQNKQHS
                     KKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVHITNKG
                     FVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQEMTFE
                     QKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRRIQEQK
                     QFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAHLSLKR
                     QLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         2422 a         1177 c         1484 g         1981 t
ORIGIN      
        1 aagcaagtaa ttcgtttcat atgaaaacaa agagggtcaa aggaaagaat gataaacgtc
       61 aggagaagag aaagcgacaa gaaaaaggaa ataaacacgt ttgttataaa atgtgaagaa
      121 aaggatgaaa aagaatccgg aataatttga atacttttat ctgaaattga atgaaaactg
      181 ttgtatctaa cgaaaaaaaa tgttaatctt tacagatgaa agggtggcat ttgttaggat
      241 tctctgaatt actaaaaaag aaaaccctca cataaaaaca acactcactt aaaggagacc
      301 gattaatatg gaaaagaaag aatttcacgt agtagcagaa actgggatcc acgctcgtcc
      361 agctacacta ttagtacaaa cagcaagcaa atttaactct gatgtaaact tagagtataa
      421 aggtaaatca gtaaacttga aatcaatcat gggcgttatg tctttaggtg taggtcaagg
      481 ttctgatgtt actattactg cagaaggtgc tgacgaagct gatgcaatgg cagcaatcgt
      541 tgaaacaatg aagaaagaag gcttatctga ataatggttg aaatgctaaa agggattgcc
      601 gctagtgacg gtgtagccgt tgcaaaagct tacctgctag ttcaaccgga tttaacattc
      661 agtaaggcaa cagtagaaga tactgcggct gaagaagctc gtttggatgc tgcattagct
      721 aaatctactg aagaattgca gcaaattcgt gaaaaagcag cgcaaagctt aggtgaagca
      781 gaagcgcaag tatttgatgc ccatttgatg gttctttcag atccagaaat ggtaggtcaa
      841 atcaagcaaa atattaaaga caacagcgta aatgctgaat cagcgctaaa agaagtaacg
      901 gatatgtata tcggtatgtt cgaagcaatg gaagataatg cttatatgca agagcgtgct
      961 gcagatattc gtgacgttgc taaacgtatt ttagcgcact tattaggcgt tactttacca
     1021 aatccatcaa tgatcaacga agaagtggtc gttgttgcac atgacttgac tccaagtgat
     1081 acagcacaat tagatcgcaa ttttgtaaaa gcgtttgtaa cagacattgg tggacgtact
     1141 tcacattctg ctatcatggc acgttcttta gaaattccag caatcgttgg gacaaaagaa
     1201 attactgcta aagtcaaaga aggcgatatt ttggctgtca acgggatcga aggcgatgta
     1261 atcatcgatc ctacagatga acaaaaagct gaatttgaaa aagcaggtgc agattacgca
     1321 gcacaaaaag cagaatggga aaaattaaaa aatgcagaaa ctgtcacagc tgacggcaag
     1381 cattttgaat tagctgcaaa catcggaaca ccaaaagatc tagttggtgt ccataataat
     1441 ggtggagaag cagttggtct gtaccgtact gaattcctgt atatggattc tccagacttc
     1501 ccaacagaag atgaccaata tgaagcttac aaagctgtat tagaaggtat ggaaggaaaa
     1561 cctgtagttg ttcgtacaat ggacatcggt ggagataaag aactaccata tctgcaatta
     1621 cctcatgaaa tgaatccatt cttaggatat cgtgcattgc gtatcagttt atctgaacaa
     1681 ggcgacgaaa tgttccgtac acaaatgcgt gcattgcttc gtgcatctgt tcatggtaat
     1741 ttacgtatca tgttccctat ggtagctaca ctcaaagaat tccgcgcagc aaaagcgatc
     1801 tttgaagaag aaaaacaaaa attgataagt gaaggcaagg aagtatctga tacgatccaa
     1861 gtcggcatca tgatcgagat tccagcagca gcagtcttag cagacaagtt tgcaaaagaa
     1921 gtagatttct tctctgtagg tacgaacgat ttgattcaat acacaatggc agcagaccgt
     1981 atgaacgaac gcgtttctta cttgtaccaa ccatataacc catcaatttt acgtttgatc
     2041 aaaaatgtaa ttgatgcagc acacgctgaa ggaaaatggg caggtatgtg tggagaaatg
     2101 gctggagatc aaacagctgt tccattgcta gtaggtatgg gtctagatga gttctcaatg
     2161 agtgcgacat ctattcttaa aacgcgtagt ttaatgaaac gcttggacac agcgaaaatg
     2221 gctgaacttg ctgatcgtgc attgaaagaa tgcgatatga tggaagaagt cgttgaactt
     2281 gttcatgaat atgtaaaata aacaacgtat caaaagctgg tgtggcttgt aaaagagcta
     2341 cgccagtttt tttgttcttt tttaagaaaa cgtttttgtc agtaagaata gacaaaaata
     2401 gggcttaagt ttgagtgaca gtttgtaggc tatgttatac tatataagtg aagggggacg
     2461 taaggtgaag gtattattat attttgaaag tgaaaagatg cttgcaaaat ctggaatcgg
     2521 gcgtgcgctt gatcatcaaa aacgtgctct gacggaagtc ggtatctctt atacactaga
     2581 tgaaaaagaa gattatgaca tcctgcacat caatacatac ggaatcaata gtcataatat
     2641 ggtcaataaa gccagaagag aaggaaaaaa agttgtctat catgcgcatt ctacagagga
     2701 agatttccga aattccttta tcggatcgaa ccagctttct cctatcgtaa aaaaatatct
     2761 ggtaggattg taccaaaaag cggactatct gatcacgcca acaccttatt caaaacagtt
     2821 gttggaaagc tatggtatcc gtgtgccgat tcaagctatt tccaatggga tcgacttaga
     2881 gaaatatcac cctgatccaa tgaaagaaca aaagttcaga gaatatttca aactgtctcc
     2941 ggatcaaaaa gtaatcattt gtgtcggttt gtttttcgaa cgtaaaggaa tcattgattt
     3001 cgtagaaatt gctaagaaga tgcccgaata cacatttatt tggtttggtc atgttcccat
     3061 gtactcgatt cctcggaaca tccggaaaat tgtaaaagaa gatcatcctg aaaatgtcct
     3121 gtttcctggt tacattcgtg gagagatcat agaaggtgcg tattcaggtg cagatctatt
     3181 ctttttccct tcttatgaag agacagaagg gatcgttgtt ttagaagctt tagcaagcaa
     3241 acaaaacgta cttgttcgag atattccagt gtataatggc tggttagaag atagcaagaa
     3301 ctgctatatg gggaagacaa acgaagaatt tatccgactg atccaacaga ttaccaatca
     3361 agaactaccg agtacgacac aagccggtta tcaaacagca aaagagcgaa gtatcaataa
     3421 aatcggtgaa gaacttaaaa atgtatacga aagtgtactg aatgaaaaag tctctagcaa
     3481 attaaaaaaa gtaaatcagg tcaaggacta gtttggaggc gaaaaacatg aaaatcggct
     3541 tttttaccga tacctacttt ccgcaagtca gcggagttgc tacatcgatc aaaacattaa
     3601 aacaagaatt agaaagacat gggcatgaag tctatatttt tacaacgacc gatcctaatg
     3661 cggataagtt ggaaaaagat gtgattcgaa tgccaagtgt accatttatt tctttcaagg
     3721 atcgaagagt tgttgttaga ggcatgtggt atgcttattt ggttgccaaa gaactagaac
     3781 tagatcttat tcatacccat acagaattcg gagcagggat acttgggaag atggtagcta
     3841 agaagttgaa aatacctctg atccatactt accatacgat gtatgaagac taccttcatt
     3901 atatcggtaa aggaaaagtg atcaggcaat cccatgtaaa atacttgtct agattatttg
     3961 caaatcatac gacaggtgta gtttgtccaa gtgaacgtgt tattgataca ttacgcagct
     4021 atggtgtaat agcaccgctt cgcgtgattc ctactggtat cgacattgaa aaattcaaac
     4081 gttctgatat cactcagcag gatatcagca aattaaggga gtcactaggt cttcaggaaa
     4141 accatttgat gattctttct ttaagcagga tttcttatga gaaaaatatc caagccttga
     4201 ttaatgggtt tcctcaggtc atctccgctt atcctaacgc cagattagtt atcgtaggaa
     4261 aaggacctta tgtagaagag ttagaggaat tgatctcaga actgcagtta gaagaatttg
     4321 tgcagttcac aggagaagtg gacaacaatg atgtagcact ttattataaa gcggcagatt
     4381 attttgtcag tgcttctact tcagaaacac aagggcttac ttatacagaa gcgatggcag
     4441 ccggaacacc ttgtgtcgtg gaaggaaatg cttatttgaa ccgattgttc gatcatcctt
     4501 cattagggaa aacttttgaa accgacgaag actttgcacc agcacttatt gattatattt
     4561 ccagtgaagt tcctaaagat ccaaaagttt tagaagctaa actttatgat atttcatcta
     4621 cacatttcgg cgaagctatg atcgaatttt atcaagacat ggttgtttat catgaagaag
     4681 agatgcgaag aaaagaagaa gaagactcga ttgaacgaat caaaataaaa ttaaattcat
     4741 ttaaaagata aaattcaaac attttttatg aaaaaatcct tgtttctagc gtatactagc
     4801 atagaagcaa ggatttttga tttttaggag gtcgaaaaaa tgaaacttgg ttttatcgga
     4861 acaggcgtca tgggaagtgc tgtcgcacgc catctactag aagcaggaca cgaagtagct
     4921 gtctataatc ggacaaaagc aaaagctgat cctttagtaa cagaaggagc tatctgggca
     4981 gatacaccaa aagcggtagc agaacaaagt aatattctct ttacaatggt aggttacccc
     5041 aaagatgtag aagaaatcta ctatggacag tcggggattt tttctgcaga tatctcagga
     5101 catattttag ttgatttgac tacaaataca ccaagcctgg cagaaaaaat tgcaaaaaca
     5161 gcaaaagaaa aaggcgcaga tgcgttagat gcaccggtat ccggtggtga cttaggagca
     5221 aagaatggta cactgacgat catggtcggt ggagaagaag ccgtttatga tcaagtactg
     5281 cctttattca aagaatttgg tacgacattt acattgcatg gttctgctgg gaaaggtcag
     5341 catacaaaaa tggctaatca gatcatgatt gcaggtacga tgacaggaat gacggaaatg
     5401 cttgtctatg cacaaaaaaa cggacttgat ttaaagaaag tgattgagac gctttcaggt
     5461 ggaagtgcag ctaactggtc tttaagcaat tattctcctc gtattttaaa agaagattac
     5521 acacctggtt tttttgtcaa acattttatc aaagatttaa agattgcatt agaagaagca
     5581 gaaaaaatgg atcttgtatt gccagcaact acccaagcat tgaaactata tgaagagttg
     5641 gcagataaag gttttgagaa tgacggaacc caagctttga tcaaactttg gtggcctgaa
     5701 ggaaaaatac cagaaaaaaa atcataaaaa acgagttttt ttagagaaaa tcaatgcaag
     5761 taggctatgc ttttgataca atataacaga aataagaaaa ggaggacctc cgatgacaca
     5821 ttcacaatta gtcgcaatca taaaacgact ggaggctatg gtcgagtcag cagataatga
     5881 actgcaagtt cgccgttttg aaaaagaagg cgtagagaag tgtacagtaa cttatgataa
     5941 agcgaccgat acatttgaat taactgaaac agatactcat cagcaatacg aatttgataa
     6001 tatcgatatc gtagcgatgg aaatctatga cttaatccaa taaattttag gataattgac
     6061 atttgaagtt gaagatgcct aaaagaagcc gagaagcaac taatttagtt gcttttcagc
     6121 ttcttttttg tttaacagtt caatttcaaa ctgttctttt tctaattttt tgcggtaact
     6181 gatcaagatt tgtacaggat cgatttcttc ttttgacaat tgacgtttca aagataaatg
     6241 agcttctttc ttttcataat agaagtcagg agaaacgtca ccagaggtaa tcattgtttc
     6301 tgcaagttga ccataacaaa cgaggtagtg ctttttctga ctctcatctg ctgctaatgg
     6361 catgctgaag gtgagcgatg gtgttcttaa aaactgcttt tgttcctgga tccgtctaat
     6421 aaaataagta actaattcga tatctttttg aagcataccg gctttttcga aattcaactg
     6481 atagatgaaa tgttgttgtc tttttttcaa gtaagtcagt agctttttat ttcggccttg
     6541 aaacagccct tggagttcct tttttttctg ttcaaaagtc atttcctgta ggtcaggaag
     6601 ctggactcgc aaagctaaca aactaatttc agacaaccaa ggcatttgat aagtttcttc
     6661 cattatacgc aaaatagacg gcattttttt atagagacga aaaggcccgt atgttcgagc
     6721 cgtaggagta tctgttaata caaatccctt attggtaata tgtacataat tataattact
     6781 gaaataattc atttgtcggt tataaaaagg acggtaatgt tggatcaatt gacactcgag
     6841 caataaagca tctaattccg tgtctactac gacgaaatca aaatctgtta tatgatgaat
     6901 catacgtaat actttttttg aatgctgttt attctgatga aaatagctgc ttactctatt
     6961 tttgagccgt tttgctttac caacatagat gaccttacca tctcggtctt tcataagata
     7021 gacacccggc agcatgggga gagttttcat tttttctttt aaca
//
LOCUS       DADXTG010000120         7046 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.122, whole genome shotgun sequence.
ACCESSION   DADXTG010000120 DADXTG010000000
VERSION     DADXTG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.122"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            668..862
                     /locus_tag="IWI57_002237"
     CDS             668..862
                     /locus_tag="IWI57_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450361.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1020..1302
                     /locus_tag="IWI57_002238"
                     /pseudo
     CDS             1020..1302
                     /locus_tag="IWI57_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1546..2376)
                     /locus_tag="IWI57_002239"
     CDS             complement(1546..2376)
                     /locus_tag="IWI57_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBC2450362.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2584..3918
                     /locus_tag="IWI57_002240"
     CDS             2584..3918
                     /locus_tag="IWI57_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2450363.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3959..4870
                     /locus_tag="IWI57_002241"
     CDS             3959..4870
                     /locus_tag="IWI57_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2450364.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4867..5721
                     /locus_tag="IWI57_002242"
     CDS             4867..5721
                     /locus_tag="IWI57_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2450365.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5718..6335
                     /locus_tag="IWI57_002243"
     CDS             5718..6335
                     /locus_tag="IWI57_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBC2450366.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6337..6855
                     /locus_tag="IWI57_002244"
     CDS             6337..6855
                     /locus_tag="IWI57_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBC2450367.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2120 a         1204 c         1401 g         2321 t
ORIGIN      
        1 cctaaccatc ttattagctt ttatagggta tatgttggtt gggctgctaa aatcatacag
       61 taatgagttg ctgaattttt caacatttat taatgataca ttagttccaa gtttgttttt
      121 cattgtattt tttgctgttg gttattttat aataaagata tgatgactaa tttagctcaa
      181 ctattccagc gtcagctgaa gatattgcaa ttcccgattc cttaatcatt tatctggatg
      241 agacagaaag tgaagttgca acagatgtgc atgtagttga ggataaaaat gatagtttaa
      301 aaattaatag ttcagatact ttactaagag taagtcctat cttttcagct gttatttaga
      361 tgcaagtagc aactaagtta ggtagtactc ctgcaatgga taggtatttg tgtgttcgat
      421 atataagtgg tcgaagtatc aaggttatgt atactggatg ggagatagaa gagttaaaaa
      481 ttggaggtca ccgccacaaa tggaatttag atttagaggt accttagatt tggtatagtt
      541 attaaacaca agatggcctg gtttgtaggg aaagtcgaag agtatttttc cacttctgcg
      601 cccctttaat ttttctagta atgccgtgaa tttgtgatta attggaaata tgaagaaagg
      661 agagtctatg aaaacaacac tagttctatt ctataaaaag catccttatt ttacacttct
      721 gataaatata ttgttggcta gtgttatcgg gatttctgtt gaatatctaa taaataaaga
      781 tttcataggt tctggttttt atacggcttt atttcttgga cttcttgaag cattttccat
      841 ttataaaaaa tcaaaaaaat aaaatagatt gccgtaagag atctttttca tttcattttt
      901 ttctgaaaac ccgttgtttc tgtcagtgaa gcagcgggtt tttagattta ttgtctttag
      961 acctaattga gcgcgccgag cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa
     1021 taaagggaaa tagtttagcc cgtacaaagt agatgtgtaa ataccacatt gtattcacac
     1081 caaagtatag aagaaaaata atttataatc aatatcgagc caatattcag gagatcatca
     1141 agagattgtg caaatctaaa atataaattt gggtatcgac acttttgggt ggaaggatac
     1201 tatgtgacaa aagtaggaca aaatgaagcg accatcaaaa agtacattca agaacaagaa
     1261 aaacatgata gtgcgttaga taagctgagt gtacgagagt aagacgatcc ttttaagggc
     1321 taaggagtag tacataaacc ttttaagagg tgggcaaaac tggcaaaggc aatatggctt
     1381 gaacataaaa tatgaaagcc agcgtcttta ggcgctggcc ggtgatttga gcttatagct
     1441 ctagagaaaa ccaccctttt gaagggtggt tatgattaat gactttagtc agttttgctc
     1501 acaaagcgtg tggaacaaaa aacgggtgtt tctacgggta gatatttatt tcttgaatgg
     1561 aggtgtccca tacatttttg gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt
     1621 aggttctttc aaccattgat attcgccttc gccatccagt gctttgccac tagcccaaga
     1681 accttcacta ctttggtctc cctctgagaa gttatagaag tcataagcaa tgtcttttcg
     1741 ttctttgtca cgcgggaaag tacttgggac taagattgaa ccttcttttt cttctaattc
     1801 tttcactgca cattcccata gattttgatg atagctatct cttgcaagga gaacagacag
     1861 catatctttt acgccacgat catcgatcat ttcgtaaata cgtgctactt gaagtcttcc
     1921 ttgtgattcg gcatttaaat tagctctaaa gtctgctaac aagttgccac tcgcaatgat
     1981 ataggcaccg ctccatggat tgcccacgct gtcagcagga cgaggtccta agccagctac
     2041 gatcgcgtgc tgtggattca ttccagagat aatggcaccg attgttggat ctttttgcat
     2101 cgctgcttct tgatcttcaa caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa
     2161 catttccacg tggccgattt cttctgttgc aatgtccatc agcatatctt tatatttttc
     2221 ttctcctcgg cagctccagc cttggaacag gtactgcata gctacactca tctcgccata
     2281 ttggccgccg atcagctcct gcagatattt cgcgattaat ggattcggac gatctggttt
     2341 tgcttcaaac tgcaattctt tctggtgtct aaacatcaaa attcctccta atctattttt
     2401 aatacattct cattctgcta cgaaagatta aaaaattcaa agaaaacgct tgcgaattat
     2461 ataaagtatt ggtatcatta ataatttttt tagaatcata ccttataaat tgttattttc
     2521 agaacaaaat gatatcgtta tattaaaacc gacatcggtt acattaaaat ttggaggtaa
     2581 aatatgaaaa aattattgtt tacagggttg ctggcaagtt cagcattgtt tttattagga
     2641 ggatgcggat caagtaatgc aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa
     2701 acaagtgatg gaagaaaaaa tattgatttc tggtcctttt ggggatccgg tgcacgaaaa
     2761 gaagctatcg aagaaatcat tgaggatttc aatcaatcac aaaacgagat ccatgtaaat
     2821 tataagtatc agccatgggg tgacatctgg actaaatctt tatctgcaat aacagctgga
     2881 aatgcacccg atgttattgt acaggacata aattccgttg cccaaagagc agaagcacag
     2941 caagcaacta atctctccaa atatgtagaa aaagacactt ctaaggattt ttatccgcag
     3001 ctttgggata cagtagaata tgaaggtgat ccttatgcga tcccattcaa tacatataca
     3061 caagtcattt tttataataa aaagttattt aaagaagctg gaatcagtga acaagatcta
     3121 ccgacaactt gggatgaatt agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa
     3181 gaatttacac gcatcggttt ttatccatta tggaatttag gggctgatgt atgggcgcta
     3241 aacgcagata acgggactag ttggtttgac aaagatgaaa aagtcaagat tgatactaaa
     3301 aataaagtgg aagccttgga gtggatccaa gactggcaag attattatgg gaaaaatacg
     3361 atcaataaat tagaagccga atttgggtct ggtgtatctg atccatttat ttccggatta
     3421 gtagccatgc gtgcccaaaa tatcaattat tactctagtt tgatggaaaa tgcaccggat
     3481 gattttgatt ttggcgtgat gcagctccca gaaaaagaag aaggttcagg ccattggtcg
     3541 tggggtggcg gattcgtctt ggaagtacca tacggagcaa aagatcctga ggcctcttat
     3601 gaattcatca aatatctttc ttctccagaa gtacaagaaa aattcggcga aaaaagtttt
     3661 gatatcatgg cgagcaagac agccaatgag aatttagtga ataatgacaa cctaacggat
     3721 aaaggaaaaa tgatctatca aatggcggac gaaaacttta aaaatacggt gatcacacca
     3781 gttcctttag atgctccgga ttacacgaca ttagtgaatg aacagattga ccagattctt
     3841 ttaggaacaa aatcaccaaa agaaggattg gcagatgcac aaaaagcagt agaaaactta
     3901 gtcaagcaaa atcaataatc catttttgac tgtctaggta cagaggaggc aagaaaattt
     3961 gaagcgttta tctatccgaa agaaaaaaga agcaagatgg gcctatttat ttatctcgcc
     4021 ttttattatc gggttcttgg tatttatgct agggcctatg cttttttcag ttattggtag
     4081 tttcaccgat tataatttga catccaagat gaatttcatt ggtctagcaa atttcaaaag
     4141 aatgttcgtt cacgatgatt tattttggaa atcgttatac aacaccgttt atttcgtcat
     4201 cttcaatgta ccgctgacga cgattttcag tgtgttttta gccacagtac tcaatcaaaa
     4261 aatcaaagga atcagtgtat tccgtacagc tttctattta ccggcagtct tatcaggagt
     4321 agcagtctac atcttatgga tgcagctgct ttctccatct gcaggattga tcaacacatt
     4381 tctgggctgg ttccatatcc ctggaccagc ttggctgttt gatccacaat ggacgaaacc
     4441 tgcgttgatt ttgatgaaaa tatggagttc tggaggagca atgctacttt acttagctac
     4501 attacaaaat gttcctagat ctttatatga atcagcagaa atcgatggag caggcgtttg
     4561 gggaaagttc aaaagtatta ctttgccatt aatcactcct attatttttt acgatgtgat
     4621 cacctctacg atcggttctt tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg
     4681 aacaggcgga cctgcaaatt ctttgctttt ttataatctg catatgtgga acaaggcgtt
     4741 cgtagctttt gatatgggtt atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt
     4801 ggttctgacg tttgtcaatt tgaagcttgc acctaagtgg gtctattatt ctggaggtga
     4861 cggcaaatga tggattattt caaaaagacc agtgtgaatc ggcaaacaaa aaaaatacta
     4921 agttatgtcc tcatgacagt aataggaatc attttgatca ttccattgct ttggatggtg
     4981 tttacttcat tgaaaccaat ggaagaaatc gttcgttatc ctccgacctt tttcccagaa
     5041 aaaatcgtat gggaaaacta tctggatacg attgccgcct ttcctttttg gcgctatgca
     5101 agaaatacat tattcatcac tgtgctagta gtgatcggta atgtcctttc aaattctttt
     5161 attgcttatg gatttgcgaa gcttgatttt cctgggaaaa agctgatgtt tgctttggtt
     5221 ctttctacga tgatgatccc ggggtttgta acgatgatcc ctcaatatgt attattctcc
     5281 aagatcggct gggtaggtac ttatttgccc ctgatcgttc catcgttctt cggcaatgcg
     5341 ttcaatatat ttctgatgcg tcaattttac ttatcgatca ataatgaatt gatcgaagct
     5401 gctgaaatcg acggagcgaa tcatttgtat atttggagtc atttgatgct tccattgacg
     5461 aaacctgcct tgattaccat tgcgatcaat tccttcaacg cagcttggaa tgatttctta
     5521 ggtcctctgt tatatatcca agatcaagaa aaatacacgt tacagattgg cttgcaagtt
     5581 tttcagaacc aagcaacgac acaatggaat tatctgatgg caggagcaac acttgtcttg
     5641 atcccgacga ttttgctgtt cttttttgca caacgttatt tcattgaagg gatggatttg
     5701 acaggaggtt caaaaggatg atgaatcgat cgattggtaa aaaagccaat gattatttct
     5761 tagaaatcat tgaatggatt ccacgtctta tccatttaca gctactatgg ttcctgtgcg
     5821 tcttgcctgt tttcacatta ggaactgcaa gtcgaacagt tctgtatatg atttatcatt
     5881 acaaaaaaaa tgaagagaaa aagctcggtt cactattctg gaaaaagttt cgtgagaact
     5941 tctctcttta tggaaagcaa gatagtttag caagtgttta ttttcttctt ctattgatcg
     6001 attatcagat tttccgctat tggggtgggg gaattggcta tacattgatg tatactacgt
     6061 tgtttttgct tctattaagc gcgcttctct ttagttacaa aatcttgctt caactggaac
     6121 aaaaagaagc ttcttggatt actgcatttt tcttgttttt taatgatttc aaaagcgcgc
     6181 ttttctattt ggcaggcacg ttgatccttc ttgtcgcatt ctggtttgca ggaccaatct
     6241 attttgcttt attaggtttc agtttcttgt tttattttca agtcctgctt tttatccatc
     6301 gtacgcaaga aaaggttctt aaaaaggagg aataacatgt tgcgtttgat tgctaaaatc
     6361 gatgatagta ttatcaacaa gttcgccaag caacggaaaa cggatagtcg aaaaatcaat
     6421 tttttgaaat ttatcagtaa tatctctaat ggtggtttta tttggttact ccttgctgct
     6481 gttttagtct tgatacctga tactagagaa tatggtctag ccattcttat cggcagtttg
     6541 atctattttt ttgttggttt gctttttatc aaaaaaacga caagacggaa aagaccttat
     6601 gaaaaaaatc gtcggcttgt cctttacaca agtaaaccga agacttcttc ttttccttca
     6661 ggacatacag cgctagcctt tttatcagta gctgcgttgt tcgaattaag tccagttgca
     6721 ggaatattag cctttttatt tgcttgtttg atcgcttatt cgaggatttt tttactttta
     6781 cattatccta ctgacatttt aggaggagct attttaggaa gtgttattgg catcctatgc
     6841 gctgccgtta tctgaattaa ttgtacgaat aaagcatgag tgcgtaaatg cgcatttgtg
     6901 ctttattttt ttgtttcgag gaaagcgtag ttgtttattc atttgtctta ataacttaag
     6961 ttttgttaaa tgagaacgtt ataaaatttt ataatttaat tttaataata aatgttgtat
     7021 aattggtgtt ttttaactaa aaagga
//
LOCUS       DADXTG010000121         7045 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.123, whole genome shotgun sequence.
ACCESSION   DADXTG010000121 DADXTG010000000
VERSION     DADXTG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.123"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            117..590
                     /locus_tag="IWI57_002245"
     CDS             117..590
                     /locus_tag="IWI57_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBC2450368.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            587..2719
                     /gene="feoB"
                     /locus_tag="IWI57_002246"
     CDS             587..2719
                     /gene="feoB"
                     /locus_tag="IWI57_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBC2450369.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2721..2855
                     /locus_tag="IWI57_002247"
     CDS             2721..2855
                     /locus_tag="IWI57_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBC2450370.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2949..3593
                     /locus_tag="IWI57_002248"
     CDS             2949..3593
                     /locus_tag="IWI57_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2450371.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3780..4973
                     /locus_tag="IWI57_002249"
     CDS             3780..4973
                     /locus_tag="IWI57_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBC2450372.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4966..6693
                     /locus_tag="IWI57_002250"
     CDS             4966..6693
                     /locus_tag="IWI57_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBC2450373.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2274 a         1229 c         1572 g         1970 t
ORIGIN      
        1 taccgagagt attctatgtg agaatgattc tcatttattt gcgtaacacg taagaaaatt
       61 agaacatata atgaatgatt ttaatacatg atttatttca caagagagga atcaatatgc
      121 agaccttagc gcaggcaaag cagaatttcg tttatcaagt cgaacgaatc gaaacagaag
      181 aaaaaacaaa acgccatttg caaaatatcg ggatcgttgc cggaagtaaa atcgtcgtcg
      241 tcaatttatc aggcaacaat gccattttac tggtaaaaag tcatcgagtg gcagtaagtc
      301 gggaaatact aggtcaaatc tttgtcaata aaaagactgc ttcctctgaa agctggactt
      361 ctcttgatca attaaaagca ggagagactg ggatcgtcgt agcaatccac ggacaaggcg
      421 cagtgaagcg acggttaatg gatatgggat tgacgcgaaa tgtaaaaata gttatccgaa
      481 aactagcccc tctgggagat ccaatcgaac tgacagtgcg agggtatgaa ctgacgttac
      541 gaaagtcaga ggccgaacta atcttgatac aaagggagga aaaagaatga atatcgcgtt
      601 agtcggaaat ccaaacagcg ggaaaactag cttgttcaat ctcttgactg gttcgaatca
      661 atatgtaggg aattggccgg gcgtaacagt agaaaaaaaa gaaggatcgt tgaaaaaaaa
      721 tccgaacatt catattcagg acctacccgg catctattct ttgtctcctt atacaccaga
      781 agaagtgatt gcaagagatt atttggttca ggaacagcct gaactgatca tcaacattat
      841 tgatgctacc aatttagaac gaaatcttta tttaactacg cagttattgg atttagacat
      901 tcctatgatc attggtctga atatgatgga tgtcatcaaa aaagaaggaa aaaaaatcaa
      961 tctagaaaag ctttcctatg gtcttggtgt accagtttta ccaatcagcg tattgaaaaa
     1021 gcaaggaatc gaaaaactaa tcgaaaagat tcaggagtct gcaaaaaagc ggccttctat
     1081 tcctgttccc tgtacatatg atccacgttt agaagcagct ttacatgaaa ttgaagatgt
     1141 actaggagaa caaagcggca agaaacgctg gtatgcaatg aaatattttg aacgagatca
     1201 gaaggtaaga gaggattacg agatagcagt ttctcagcaa aaagagatcg agcaactgat
     1261 ccagttaaca gagaaactat tggacgacga tagtgagacg attctggtca atgaacgtta
     1321 tgaatttatt acccaactat gtacactctg tgttgtgtca aatgattctt tccaattatc
     1381 gatgagtgac aaaatcgatc agattgcaac gaatcgttgg ttagccttgc cgatcttcgc
     1441 atttgtgatg tggctgatct attatctggc tattcaaaca gtaggaacaa tggggacaga
     1501 ttggatcaat gacacattgt ttggtacatg gttaccagaa catgttggca gattgttagc
     1561 tgaatggcaa gtagcgggat ggatgcagga gctgatcttg aacggcatca tcgcaggcgt
     1621 gggagcagtg ttaggatttc taccgcaatt gattatattg tttttatgct tgagctttct
     1681 ggaagactgc ggatatatgg cgaggatcgc ttttgtcatg gatcgacttt tcaggaaatt
     1741 cggtctttca ggaaaatcct tcattcctat gctgattgct acaggatgtg gagtgccagg
     1801 cgtcatggcc agtcgcacga ttgaaaacga gcaagatcgt cgtttgacga tcatggtcac
     1861 aaccttcatg ccttgttctg caaagttgcc gattattgcc ttagtagcag gggccttttt
     1921 tcctcatcaa tcttgggtag ctccttctgc ctattttctt ggtatgactg caattatttt
     1981 ctctggaatc agcttgaaaa aaacacgttt gtttgctgga gatacagctc cttttatcat
     2041 ggaattgccc gcatatcatt ttcctcaatg gtcagcggtc ctaagacaaa cctatgaccg
     2101 cagcaagtct tttgttaaaa aagcaggaac gattatcttt gtttctagta tcgtgatctg
     2161 gttcagctct tcttataatt tttatgctca acctgtttct gaagaccaaa gtatcttagc
     2221 ctttttcggg cgaatcctgg cagttgtttt tcagccgctt ggttggggaa actggcaagg
     2281 aacagtcgca gcgattacag gattggtcgc taaagaaaat attattggca catttggtat
     2341 tttgtttggt catgcggaag tatccgaaaa tggcagagaa atctggcaag tgctacaaca
     2401 cacatatact cccgcagcag cttattcatt gttagccttc aatttacttt gtgcgccttg
     2461 ttttgcggca attggggcta tccatcggga aatgaatgcc aagaaatgga cttggatcgc
     2521 cataggctat caatgcggtt tagcttattt agttagtttt gtgatttatc agttgggaca
     2581 cttattggag ggtggacagg tagcgttagg gacgtatctt gctattttac tgatgatagg
     2641 actcgtttat gttttgttcc gtcagccaaa atccaagaac cagttttata ctatattgtc
     2701 attagaagga gaggaatagg atggcgacat tcttattaag tgtgttaatc tttggaagtg
     2761 caggcgcaat tgtctatact cgtttaaaat ccggtaagaa ttgtgatgac tgtcaaacag
     2821 cttgcccagt aaaaaaggaa cagatcaagc gatgatcagt cgttgcaaga ggctattctc
     2881 cccttttttg cttattttgc tagagcatag taagatgagg gaaagtaaaa aaagaaagag
     2941 gaaagaaaat gtctggaaag aaaaaaatcg tccgcccgcg ctaccagcag attgctgttg
     3001 atttagccga acgtatcgtt gaaaaccgat ataaagtggg tgagaagatc catgcccgct
     3061 caacacttgc cagtacgtac aatgcttctc ctgagactac ccgcaaggct atcaatgtgc
     3121 tggtagatct tggaatcatg gaagtacggc atggaagtgg ggcatttgtt gcttcgaagg
     3181 aaaaagcaaa agatttccta ttgcaatatc aagatgtaca atccatccaa gatacaaaag
     3241 cagaaatcat ggcaagtgtg gagaagcaac aagatgagct gaatcatttt tctcaattat
     3301 tggatcagct ggtcaatcaa acgaagcgaa tacataagat gaatccttta cttccgtttg
     3361 aattatcttt atcagaaaaa gcaatgcatc tggaccgttc aattagcgag ttgaatatct
     3421 ggcaggcgac tggcgccaca gttatcgcaa tttctcataa tgaagaactg cttgtttcac
     3481 ctggccccta tgctaaattt acggcagggg acatcgttca ttttattgga aatgaatata
     3541 cactacagcg tatgacaaac tttttttacc cggagcatca gcttgcagaa tgattgtgtg
     3601 aaatattaaa aaaatattaa cttggctaaa ccttttattt acaaagattt agccaagttt
     3661 ttttcagttt ggacaagcaa atttctttca tttttgacaa agtgactaca tgatgttaac
     3721 atagtgtggt cacttttttc agaaagaaaa atgaatttac ataaaaaagg agaatttgtt
     3781 tggtaaaagt agaagtaaaa catttaacaa agatatttgg taaaaagaca caagccgctt
     3841 tagatatgat gaatgatcac cagccgaaaa cggagatttt aaagaaaacc ggtgcaacgg
     3901 ttggtgtgta tgacgtgaat tttgacgtaa aagaaggaga aatcttcgtg atcatggggc
     3961 tttccgggag tgggaaatcc acattgatcc gtctattgaa tcgtctgatt gagccaactt
     4021 cgggcagtat ctatattgat ggagaagatg tatcgaagtt aagcaaagaa gaattgcgag
     4081 aagtccgtcg tcataaaatc aatatggttt tccaaaactt cggtctgttt ccacatcgaa
     4141 cgatcttgga aaatacagaa tacgggttag aagtccgtgg tgtacctaaa gaagaacgac
     4201 aagaaaaagc agaaaaagca ttagaaaatt ctagtttgct ttcatttaaa gaccaatatc
     4261 ctagtcagtt atctggtggg atgcaacaac gggttggcct agccagagct ttagcaaatg
     4321 atcctgagat cttgctgatg gacgaagcgt tctcggctct tgatccgctg atccggcgag
     4381 aaatgcaaga cgaattactg gacctgcaag ccaatgttca aaaaacgatt attttcatta
     4441 cccatgattt gaatgaagct ttacggattg gggacagaat cgccttgatg aaagacggcg
     4501 aaatcatgca gatcggaaca ggtgaagaaa tattgaccaa tccagcaaat gattatgtac
     4561 gtgaatttgt ggaagaagtc gatcgttcca aagtgcttac ggctcaaaac atcatggtac
     4621 ctgcattgac gacgaatatc gaatctgatg gaccaaacgt tgcattgaca aggatgcgta
     4681 atgaagaagt cagcatgctg atggctgttg acagaaagag acacctgaaa gggatcatca
     4741 ctgcagatca agcgttagaa gctaggaagc aaaaacgtcc attgatcgac ttcctagatg
     4801 aaaatgtgac ggtgattggg aaagacatgg tggtaagtga tattttcaat attatttatg
     4861 actcaccgac tccacttgca gttgtagagg atggaaaatt aaaaggtgtc gtcatccgag
     4921 gtagtgtgat tgaagcatta gcagaaacaa gcgaggtgag tgaacatgaa taatctattg
     4981 aactatcaat taccagtggc aaactgggta gaaaatatca cagaatggtt tacgacaact
     5041 ttttctggcc tattttcatt tttacaaacg attggacagg cagtgatgtc ggggattaca
     5101 aatttactgt tagtgattcc agctcctttg tttatcttgt tattgacggt agcagctttc
     5161 tttatttcta agaaacgtcc aggacttacg ttgttcacat taatcggtct atggtttatt
     5221 tataaccaag gtctatggaa tgatttgatg aatactgtca cacttgttct tttatctagt
     5281 gtgatctcga tcatcattgg ggtaccatta gggatcttga tggcaaaaag cagtaaagcc
     5341 caaagtatta tcaaaccgat ccttgacttt atgcagacaa tgccaggatt cgtttatttg
     5401 attccagcag ttgcattttt cggtatcggg atggttccag gggtatttgc ctcagtcatc
     5461 tttgctctgc caccaaccgt tcgttttacg aatttaggga ttcgacaagt tcctaaagaa
     5521 ttagtagaag cttctgattc attcgggagc acaagtcgtc aaaaactatt caaactagaa
     5581 ttgcctttag ctaaaagtac gatcatggca gggatcaatc aaacaacaat gctgtcactt
     5641 tcaatggtcg ttattgcctc tatgatcggt gcacccggct taggacgcgg cgtattatca
     5701 gcattgcaaa gagcacaagt cgggaatgga tttgtcaacg gggttgcatt agttatctta
     5761 gcaatcatca ttgaccgttt tacacagcat ttgaaccagc caaacaataa aaaagctgca
     5821 ggagcaacaa cacagaaaag taaaaaacgt caaggactga tcattggtgc tgtagtagta
     5881 gtgatcttag gtgcgattgg tatagggagt ttttcatctg ccaaagaaac aaaacgtatc
     5941 aacttatctt atgttgaatg ggatacagaa gttgcttcaa caaatgtagt cggtgaagta
     6001 ttgaaacaaa tgggctatga tgtaacgatg acgccattag ataattcaat catgtggaaa
     6061 tctgtttcaa atggcgagtc agatgccatg gtttcagctt ggctacctaa aactcatggt
     6121 tcacaatatg cacaatataa agatcaagtc gaagacctag gtgccaattt gacaggagca
     6181 aaagttggtt tagcagtgcc agcctacatg gatgtaaatt cgatcgatga gttaaccgat
     6241 caggcaggta agaaaatcat aggaatcgag ccaggagctg gtgtagttac ggctgctgaa
     6301 aacacgatcc aaaaatatga caacttgaaa gactggaaag tagagacatc ttcttcagga
     6361 gcaatgaccg ttgcactagg tcaagcgatc aaaaaacacg aaccaattgt agttacagga
     6421 tggacacctc attggatgtt tgccaaatat gatctgaaat acttagaaga tcctgaaaac
     6481 ggaatgggaa gcgaagagca gatccatacg atggttcgaa aaggattgaa agaagaccag
     6541 ccagaagctt acaaagtatt agacaatttc cattggtcag aaaaagatat ggaaaaagtt
     6601 atgttggaaa tcaacaatgg aaaagatcca cagcaagcag caaaagactg gatcaaagag
     6661 aaccaagaat tagtcgaaag ctggaaaaaa taaaaagaca gacacgaaat aaatggagct
     6721 tccaaaattg gatcaaatat ccaaatttgg aagctccatg ttcgtctcta tcttttttgt
     6781 ctataaaaaa agagaatgcg tagctcatct tcgaaacgtt caaagacgcc gacaaactat
     6841 tatgaattta tgggtgcgag tcaaagagat cgatcgtagg tatgaaagac taatgtgatt
     6901 aaagtttatt atgtacgaag tgcattctca aaaaatatta aagaatatac atagtttata
     6961 tacttaaaag tgattaaatt cttattcagg aattattttg ctacgtttca agaaggaatt
     7021 gttttttgaa atttaatgaa tgtta
//
LOCUS       DADXTG010000122         6976 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.124, whole genome shotgun sequence.
ACCESSION   DADXTG010000122 DADXTG010000000
VERSION     DADXTG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.124"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..565)
                     /locus_tag="IWI57_002251"
     CDS             complement(107..565)
                     /locus_tag="IWI57_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2450374.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(578..1909)
                     /locus_tag="IWI57_002252"
     CDS             complement(578..1909)
                     /locus_tag="IWI57_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBC2450375.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1920..3362)
                     /locus_tag="IWI57_002253"
     CDS             complement(1920..3362)
                     /locus_tag="IWI57_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2450376.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3349..4113)
                     /locus_tag="IWI57_002254"
     CDS             complement(3349..4113)
                     /locus_tag="IWI57_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBC2450377.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4217..5092)
                     /locus_tag="IWI57_002255"
     CDS             complement(4217..5092)
                     /locus_tag="IWI57_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2450378.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5347..5431)
                     /locus_tag="IWI57_002256"
     tRNA            complement(5347..5431)
                     /locus_tag="IWI57_002256"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5395..5397),aa:Leu,seq:caa)
     gene            complement(5449..5520)
                     /locus_tag="IWI57_002257"
     tRNA            complement(5449..5520)
                     /locus_tag="IWI57_002257"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5486..5488),aa:Gln,seq:ttg)
     gene            complement(5530..5602)
                     /locus_tag="IWI57_002258"
     tRNA            complement(5530..5602)
                     /locus_tag="IWI57_002258"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5567..5569),aa:His,seq:gtg)
     gene            complement(5664..5748)
                     /locus_tag="IWI57_002259"
     tRNA            complement(5664..5748)
                     /locus_tag="IWI57_002259"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5712..5714),aa:Leu,seq:caa)
     gene            complement(5769..5839)
                     /locus_tag="IWI57_002260"
     tRNA            complement(5769..5839)
                     /locus_tag="IWI57_002260"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5805..5807),aa:Cys,seq:gca)
     gene            complement(5885..5956)
                     /locus_tag="IWI57_002261"
     tRNA            complement(5885..5956)
                     /locus_tag="IWI57_002261"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5922..5924),aa:Gln,seq:ttg)
     gene            complement(5968..6040)
                     /locus_tag="IWI57_002262"
     tRNA            complement(5968..6040)
                     /locus_tag="IWI57_002262"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6005..6007),aa:His,seq:gtg)
     gene            complement(6043..6116)
                     /locus_tag="IWI57_002263"
     tRNA            complement(6043..6116)
                     /locus_tag="IWI57_002263"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6082..6084),aa:Trp,seq:cca)
     gene            complement(6123..6203)
                     /locus_tag="IWI57_002264"
     tRNA            complement(6123..6203)
                     /locus_tag="IWI57_002264"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6167..6169),aa:Tyr,seq:gta)
     gene            complement(6205..6280)
                     /locus_tag="IWI57_002265"
     tRNA            complement(6205..6280)
                     /locus_tag="IWI57_002265"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6245..6247),aa:Phe,seq:gaa)
     gene            complement(6316..6388)
                     /locus_tag="IWI57_002266"
     tRNA            complement(6316..6388)
                     /locus_tag="IWI57_002266"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6353..6355),aa:Asp,seq:gtc)
     gene            complement(6398..6470)
                     /locus_tag="IWI57_002267"
     tRNA            complement(6398..6470)
                     /locus_tag="IWI57_002267"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6435..6437),aa:Val,seq:tac)
     gene            complement(6486..6557)
                     /locus_tag="IWI57_002268"
     tRNA            complement(6486..6557)
                     /locus_tag="IWI57_002268"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6522..6524),aa:Glu,seq:ttc)
     gene            complement(6572..6661)
                     /locus_tag="IWI57_002269"
     tRNA            complement(6572..6661)
                     /locus_tag="IWI57_002269"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6625..6627),aa:Ser,seq:gga)
     gene            complement(6701..6774)
                     /locus_tag="IWI57_002270"
     tRNA            complement(6701..6774)
                     /locus_tag="IWI57_002270"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6738..6740),aa:Asn,seq:gtt)
     gene            complement(6784..6899)
                     /gene="rrf"
                     /locus_tag="IWI57_002271"
     rRNA            complement(6784..6899)
                     /gene="rrf"
                     /locus_tag="IWI57_002271"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2092 a         1546 c         1145 g         2193 t
ORIGIN      
        1 aacaaaaatc aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt
       61 aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac
      121 aactatcttt aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg
      181 gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa
      241 tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac
      301 ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac
      361 tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg
      421 catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa
      481 agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa
      541 tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt
      601 ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg
      661 tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta
      721 ctagtaactt ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg
      781 gttttcttag tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa
      841 ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac
      901 gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa
      961 aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg
     1021 tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct
     1081 caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa
     1141 aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat
     1201 taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt
     1261 ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat
     1321 acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg
     1381 aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt
     1441 cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt
     1501 gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc
     1561 cgagtccaca cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt
     1621 ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag
     1681 caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca
     1741 taaatttaac aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta
     1801 tttcagataa tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat
     1861 acgtactccc gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc
     1921 tattcttcta ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata
     1981 atttgataat ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc
     2041 tgttcctcga ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag
     2101 acatccagga tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca
     2161 taatttttac ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct
     2221 gccttagctt cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta
     2281 acgtaataca aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg
     2341 attcgaaaat tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata
     2401 ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac
     2461 ataggagaaa gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa
     2521 gttcctgcac cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct
     2581 ttatagatag ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat
     2641 aaaaacagtc cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact
     2701 tccttgctag gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg
     2761 tgcagtccaa aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct
     2821 cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata
     2881 aagcccaaaa aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt
     2941 tcgcccgcaa aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc
     3001 catgccgcta ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc
     3061 ataatttcta agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt
     3121 cctttgtcct tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag
     3181 tttccaaaaa cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct
     3241 gataaacgca tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt
     3301 ccaagcttct gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc
     3361 atatgttact aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat
     3421 taaaaattcg acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg
     3481 atccaaatac gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag
     3541 taaacttgcc aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc
     3601 tcctacgaca tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc
     3661 aaagttgctc cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt
     3721 ttcatccaaa gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg
     3781 ataaattttt tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa
     3841 tggatgctga taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt
     3901 tttgaggagt ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat
     3961 ttcctttctt agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga
     4021 tgtcaaaatt cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc
     4081 aaaatcatcc gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa
     4141 ataggcgcaa ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga
     4201 tagttacgcc cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat
     4261 tttcttgaat ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac
     4321 aatacgcttt gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt
     4381 cttcctgata aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag
     4441 tcagtgattc tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg
     4501 tcgtcatttc tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa
     4561 tgatatgcat agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat
     4621 tgattcgctc gaccacttct actgctatat catattcatt tggataaagg aatctcattt
     4681 ctggcaacat aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa
     4741 tatgatctgt caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta
     4801 tcagttcaat cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct
     4861 cactgaatcg tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa
     4921 catcaaatgg cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt
     4981 ctattccatc gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg
     5041 ttaattcacc tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt
     5101 ttgtctccaa acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc
     5161 gtgcctaatt gaatagtagc accctacata atttacaaaa atagtttaac acataataaa
     5221 agcgttatca atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga
     5281 ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga
     5341 taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt
     5401 ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg
     5461 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     5521 agaataaagg gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg
     5581 ttaaccactt cgccacattc gccatcataa aagatgttgt ttaatttctc aataaaaaac
     5641 taagatgaag acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg
     5701 ggcactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat
     5761 tagtactaag gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt
     5821 accacttggc tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata
     5881 gaaactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
     5941 gcttggctat accccaagaa taaaaagggc gaatgatggg aatcgaaccc acgaatgccg
     6001 gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catggcaggg gcagcaggaa
     6061 ttgaacccac actaacggtt ttggagaccg ctgttctacc tttaaactat gcccctataa
     6121 aatggaggag agtggattcg aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg
     6181 tttagccact tcgctactcc tccatggtgg cgcgggacag aatcgaactg ccgacacacg
     6241 gagcttcaat ccgttgctct accaactgag ctaccgcgcc atattttaaa attattaatt
     6301 gaataacaag aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca
     6361 ggcatgttaa cccctacacc acggaaccag ttcattatgg aggttaacgg gatcgaaccg
     6421 ctgaccctct gcttgtaagg cagatgctct cccagctgag ctaaacctcc aacattcaat
     6481 taaaatgacc cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac
     6541 cgcttgacca acgggccagg taaatttgct acggagagta agggattcga acccttgaga
     6601 cagtttggca ccatctacat gatttccaat catgctcctt cggcctctcg gacaactctc
     6661 cagagtgaaa gaagctcaat actctttctc aaaatgagaa ctccggcagt aggactcgaa
     6721 cctacgacat catgattaac agtcatgcgc tactaccaac tgagctatgc cggaataaca
     6781 tcagcgtggc gacgtcctac tctcacaagg ggcaacccct cactacaatc ggcgctaaga
     6841 agcttaactt ctgtgttcgg catggttaca ggtgtatcct tctcgctatc gccaccacac
     6901 tgtggtgtta tcttttattg agtaaatttt gttcactcaa aactggattt gaagtcacat
     6961 aagttttttt ccgagt
//
LOCUS       DADXTG010000123         6792 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.125, whole genome shotgun sequence.
ACCESSION   DADXTG010000123 DADXTG010000000
VERSION     DADXTG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.125"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..384
                     /locus_tag="IWI57_002272"
     CDS             <1..384
                     /locus_tag="IWI57_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBC2450379.1"
                     /translation="GQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYHK
                     AAACTSEACDFRLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLKE
                     DFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            400..549
                     /locus_tag="IWI57_002273"
     CDS             400..549
                     /locus_tag="IWI57_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450380.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            630..1226
                     /locus_tag="IWI57_002274"
     CDS             630..1226
                     /locus_tag="IWI57_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBC2450381.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            1239..2138
                     /locus_tag="IWI57_002275"
     CDS             1239..2138
                     /locus_tag="IWI57_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBC2450382.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            2141..2626
                     /gene="ssb"
                     /locus_tag="IWI57_002276"
     CDS             2141..2626
                     /gene="ssb"
                     /locus_tag="IWI57_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBC2450383.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2992..3252
                     /locus_tag="IWI57_002277"
     CDS             2992..3252
                     /locus_tag="IWI57_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450384.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3699..3905
                     /locus_tag="IWI57_002278"
     CDS             3699..3905
                     /locus_tag="IWI57_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBC2450385.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3905..4129
                     /locus_tag="IWI57_002279"
     CDS             3905..4129
                     /locus_tag="IWI57_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450386.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4144..4581
                     /locus_tag="IWI57_002280"
     CDS             4144..4581
                     /locus_tag="IWI57_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450387.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            4574..5281
                     /locus_tag="IWI57_002281"
     CDS             4574..5281
                     /locus_tag="IWI57_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450388.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            5662..5841
                     /locus_tag="IWI57_002282"
     CDS             5662..5841
                     /locus_tag="IWI57_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450389.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5934..6119
                     /locus_tag="IWI57_002283"
     CDS             5934..6119
                     /locus_tag="IWI57_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBC2450390.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            6153..6584
                     /locus_tag="IWI57_002284"
     CDS             6153..6584
                     /locus_tag="IWI57_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBC2450391.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         2437 a         1141 c         1402 g         1812 t
ORIGIN      
        1 ggacaaatcc aatccactga ttttggatcg acgttacaag aggtgaaagc agccagtgaa
       61 aagcaagaag ccgcgcgtca tttagggatt tgtccaaaat gccaggaaca agaagtttta
      121 ctctatcata aggcggccgc atgtacgtct gaagcttgtg attttagatt atggacaacg
      181 attgccaaaa agaaactcac agcgacacag ctgaaggaaa tcattcaaaa tggccgaaca
      241 agccagccag tgaaaggatt aaaaggtcaa aagggttcgt ttgaagcaac gattgttcta
      301 aaagaagact tcacaacgag ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa
      361 agaacacgcc gcactacaaa ataatggagg ggttatctta tgcgaaatac aacacacaaa
      421 caacaagaaa tcgaaaatca aaaagcaaaa attcgttcag aacaagagaa attacgtgcc
      481 agtaaaagaa gacttcagca attaaagaac cagcaacgga ttcaaaaaga aattgaatta
      541 gatcgataaa atcttcctgg ctattgtata cgcataaact tatgttatac tatatttata
      601 agtttatacg tatacaaagg agggagaaaa tgacaaaagc aatggtttac aagctgctag
      661 aagaatttaa cggaactttg gctttgaagg atgccaaaaa agcgggcatt tctcctgtaa
      721 cgatcaaacg tatggtagat cgtggagagc tggatcgtga gtatccaggt tttttcacct
      781 tacctggtca atttcctgat gaattgttta tggcacaaaa gaaatatgag cgcggcatta
      841 tttcgcatat caccgcgttg gatttatatg atttaacgga tatgatccct agacagatcg
      901 atctaaccgt accttacggt tatcacgttt ctgaaaaggg gttgaaagaa ttcgcagttg
      961 agttacacta ttcaaaagcg gaatggtatg aattaggtaa gatcgaaata aagagtcgtt
     1021 atgggaatcc tgttatagct tatgatccag aaagaacctt gtgtgacatt tggaatccct
     1081 ggtacaacgt tgaggatgaa atcaaagtaa aagcaatcaa aaactacatg gagtcggaca
     1141 gaaaaaatct tagaaaattg aatgagtatc gacgaatttt gcctacagac aaaacgatac
     1201 gctcttatat tatggcttta aattaaagga gaagacacat gacacctacg caattaaaag
     1261 caaaagtgaa gaatatttcc agagaaaaag aagttgatcc gcagcttgtt ttaagacatt
     1321 ttatgatgga aaaattcttg gaaaaaatct cagagtctcc ttatcgtgaa aattttgtgt
     1381 taaaaggcgg cttcttaatt ggctcaaagt atggcattga aaatcgaaca acaaaagaca
     1441 ttgatacgac tttacgggaa atgaaagtaa ccaaagaaac attgacaacg gttctaaacg
     1501 atattttttc aacgccgacg aaagaaggca ttcaatttga gattcaagga atgaaagaaa
     1561 caagggaagc tgattattat ccagggttta gcttacgtgt actggcacat ttggaaaaca
     1621 tgcggccaga ttttaaggta gacgtgacga caggtgactc gatctatcct gcaacgatca
     1681 ctcacagtca taaattgatg tttgaaaatc gaacgattcc tttggaatct tatcctacag
     1741 aacaaattat tgccgaaaaa ttaagtgcaa cctttgattt tttaacagat aattcgagag
     1801 gaaaagattt ttatgatctc tatactattc ctaaaatgga aaagatcgat gagaagacct
     1861 tgaaagatag tgtgagaaat acatttacac gtcgaggaaa aactgatccg ttaaagattt
     1921 attacgaaaa ggatatggat gtactgaaaa atagtcatga gctaaaagat acctggaaga
     1981 aatatcaaaa aagcaatcct tatgctgcgt cgatcacgta cgaagagaca atggattcga
     2041 ttagtaattt gatggaaaaa gtgattcacg tcgaagaaaa agagcgaaaa atagcttatc
     2101 aaagacacca acagatgaac agagaaatgg agagataaaa atgatcaata atgtaacttt
     2161 agtaggacgg ttaaccaaag atccagattt acgttatacc gcaagtggca cagcagtggc
     2221 tacttttacc ctagccgtta atcgtaattt cacgaatcaa aacggcaatc gagaagctga
     2281 ttttatcaat tgcgtgattt ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa
     2341 aggcatcttg atcggcgttg tcggacggat tcaaacacgg acttatgaca accagcaagg
     2401 acaacgtgtg tatgtgacag aagttgtagc tgataacttc caactattag aaagcaaagc
     2461 agcgacagag agccgtgcgc acgctgatca atcgtccacg agtccaagca ccaccacttt
     2521 tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac agaatccatt
     2581 tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttttaagtag cattttctat
     2641 tggttgcatt tgctacggtt tatgttataa tttagtcaac aaaaagaatt ttcaatctat
     2701 aaccaaaaac cactagctgg cgggctagtg gttttctttt acgtttctat tataagaatt
     2761 aacgagtagg cttactgtct ttcttggcaa atttacaatc aagccatttg cagatatagt
     2821 gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc aaacctataa
     2881 cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta tagcataatt
     2941 gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt gatgaacaaa
     3001 tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt ggttcgcttt
     3061 actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga aattatcgat
     3121 aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca cttcaataaa
     3181 cgaaatcagc tggttattgc tagcatgtat gttagaaatc ccgatcattt tacacgatcg
     3241 atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc tctttttgtt
     3301 ttacatagaa acgggaacag gaaaaggcct gtttaggaag gcacaagcgc cttctcaatt
     3361 aaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgct
     3421 gcacgttcac taagatatgg tgtcttgcaa agcaagacaa aataggaaaa gcttcctctc
     3481 accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg gcaacaagtt
     3541 gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg cgcccttgtc
     3601 cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa taaagaaaga actaacgaaa
     3661 ggaagtaaaa cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt
     3721 ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact gtcgaatgcg
     3781 attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt ccaacatgcg
     3841 ggaaaaaata taccttgtct tatggcgatt acattttgat tgatgagaaa ggaaagaaac
     3901 gctgatgaaa acacaacaca aattattgaa tgtaaaagaa gtcatttctg aaaaagtagc
     3961 agaagcttta cttattccgc ggtactggac gatagaaacc cctttagtag aggacgcacc
     4021 gactatgcta tttgaagacc tagtgaagat tgcaggatat gaactgcatt atcaagaaaa
     4081 cttggaaact tatgaaaacg aatattggtt aaccaaatta gagaactaaa aaggagaaaa
     4141 aatatggctc agaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
     4201 aaaatggagg atatgacgtt cctataccat gaaacacgtg tacccaaaaa acattacaag
     4261 gaaaaattgt cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt
     4321 gcgacgtact atcgaacatt ggaagaactg aaaaaacaaa atggaaaatg gtttttccaa
     4381 gccttactat gcttggaagc aggcgtcaaa ccaagcacaa tcaaaccttc tgaataccaa
     4441 gctttagaac taacgtatgg aaaatttatc gaaacgaaaa aagccaagac tgtttcaagt
     4501 gaatggttgg actactttga aaatgtcaac aagtatggcg catgttacat catgaaaaag
     4561 gaggacaacg aaaatgagta atgtggttta tcaactaaca gtggaagatg tttctcactt
     4621 tttaacaaac aaagaacatt tgaatgaagt agtcagacgg attcaaaaag actttagttg
     4681 tatggaagaa atgaagtact ttattcaaag agtggaagaa cagttgtata tggaaagcga
     4741 aacgagtcaa acgaaggtca cagtcagcca taatcattta gacgtgtata aacgtcgtcc
     4801 tgaaacggta aaaaaaggaa cagagcttct acgactagaa acagaaaaat acatcatgaa
     4861 tgtgtaccta gaaaggagtg agctggtcga aaactttgtc agctacacga tttgtaggaa
     4921 acactattac tcagaatcag aatattattc aatttttgag agcatggaat taatggaaga
     4981 agaaatggaa gagctaaaca gcaaaaatgc gacggtagac gattattacc ttgtatttac
     5041 tagatttctg acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcgc
     5101 tgtacccgaa gcatttttga gggaatatgt agaagaagta gataacccag gcgacgactg
     5161 gtacacgact ttaactgatt ttttagatac ctacacttac gacgatacag aagaaatcca
     5221 aaatgagctt gactgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtcaata
     5281 accatttata ccgaaaagtg agaatgtgtg aaataagaac aaaaaacctt gaaattaagg
     5341 gaatggttct tatttcgcac caatcgctcc aaccgccccc gccgctcagg tggtgcgaaa
     5401 taagcgggca acgaccgata acctaagaga aagaaggaaa tccatatgaa taaaatcatt
     5461 cctctgttgt tcgtactgtt tgttggcgag atacttatgc gtcatgctgc agctgtacat
     5521 ttaaacttcg tagtattctt aggaatactg gtattttgtt tgatcttttg gcgatttgta
     5581 ttagcccttg tgagtctact attcaagatg attgcagcca tatttttcat agggttattt
     5641 cgatgaaagg agagagtact aatgccaatt gcaataggaa ataaacgttt acccgtgacg
     5701 ttggatgaaa agagacaaaa agaattgcag gaactcaaac agaagtacgg caaaagtgaa
     5761 tccaggatta tgtgtattgc gttagattta ttgattgcac aagaaaaagc agggtttgat
     5821 gtaccagcac tcaaaaagtg acgtcacttt tcatttgaag aaacaaaagt gatagcactt
     5881 tacgcacgtg tcgatacgtg ttatgattat tttgtaagga ggtatagtgc acaatgccac
     5941 ttacgggaaa agaaatgctt aagttattga aaaaaaatgg ctgggttgag cgaaggcaag
     6001 agggatctca tcatcatctt tataaggatg gtgttagaat cactgttcct gttcatgcca
     6061 atcaagatct tggccgagga cttgagcaaa aaattttaaa ggatgcggga ttgaaataat
     6121 ctcgcattcg attaaagaaa ggattgactg aaatgttaag tgagccttta gcaaaaacct
     6181 atcctgctat ttttagccca gaagaaggtg gtgggtactt tattgaattt cctgatgttc
     6241 aaggtgctta cacaggaatt aacgaagacg atatatctta tggaattgct atggcacaag
     6301 aggtgttagg tatggtttta gcagattata ttgagcatga agatctactt ccagaaccaa
     6361 caccaatcaa taagatttct gttgaagatg attcttttac tactttaatt cgtgtagatg
     6421 ttgccaaata tttgaaagac acagaacttg tcaaaaaaac cttaacaatt cctcaatggg
     6481 cggataagct aggcaaaagg gctggcataa atttttcagt ccttctaaca gaatcaattg
     6541 cagataaagc agataacatt cttcgttctg gaagaaataa ttagaactca aaagagtcag
     6601 aaacttaaaa atctttggct tcttttttta ctacaaagat ggcttcttta aattatttgc
     6661 ctgattgatt taaaaaaatt gcgcaaagac tacgagctaa ttaagttgct cgtggtcttt
     6721 ctttatgaaa actttttaga tctagataaa aacattgaga cactttgaaa aattttttta
     6781 atagtaatgg gc
//
LOCUS       DADXTG010000124         6665 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.126, whole genome shotgun sequence.
ACCESSION   DADXTG010000124 DADXTG010000000
VERSION     DADXTG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.126"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(718..3867)
                     /locus_tag="IWI57_002285"
     CDS             complement(718..3867)
                     /locus_tag="IWI57_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBC2450392.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="IWI57_002286"
     CDS             complement(3880..5079)
                     /locus_tag="IWI57_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBC2450393.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="IWI57_002287"
     CDS             complement(5069..6664)
                     /locus_tag="IWI57_002287"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBC2450394.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1856 a         1374 c         1091 g         2344 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaa
//
LOCUS       DADXTG010000125         6325 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.127, whole genome shotgun sequence.
ACCESSION   DADXTG010000125 DADXTG010000000
VERSION     DADXTG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.127"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWI57_002288"
     CDS             complement(99..1229)
                     /locus_tag="IWI57_002288"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBC2450395.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="IWI57_002289"
     CDS             complement(1878..3080)
                     /locus_tag="IWI57_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2450396.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWI57_002290"
     CDS             complement(3140..3343)
                     /locus_tag="IWI57_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBC2450397.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWI57_002291"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWI57_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="IWI57_002292"
     CDS             4880..5692
                     /locus_tag="IWI57_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450398.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQNQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1884 a         1299 c          995 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attagccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caaaaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaa
//
LOCUS       DADXTG010000126         6203 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.128, whole genome shotgun sequence.
ACCESSION   DADXTG010000126 DADXTG010000000
VERSION     DADXTG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.128"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..936
                     /locus_tag="IWI57_002293"
     CDS             19..936
                     /locus_tag="IWI57_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2450399.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3116
                     /locus_tag="IWI57_002294"
     CDS             939..3116
                     /locus_tag="IWI57_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBC2450400.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3113..4114
                     /locus_tag="IWI57_002295"
     CDS             3113..4114
                     /locus_tag="IWI57_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBC2450401.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4111..5043
                     /locus_tag="IWI57_002296"
     CDS             4111..5043
                     /locus_tag="IWI57_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2450402.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5288..5404
                     /locus_tag="IWI57_002297"
     CDS             5288..5404
                     /locus_tag="IWI57_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBC2450403.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5420..>6203
                     /locus_tag="IWI57_002298"
     CDS             5420..>6203
                     /locus_tag="IWI57_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBC2450404.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKK"
BASE COUNT         2001 a         1161 c         1300 g         1741 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat
      121 tctgaatcac tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg
      241 ttgatgaaag tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcacatt tattattcag taatggttat
      361 gtggagcagt ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta
      421 cttcccgaca aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct
      481 atgctgattg tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc
      541 aagattgtag atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta
      601 tctatgaagc tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagacg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt
      721 cgttccactg accttaacga gattaaaaag accttagcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga
     1201 ttggaaaaca ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt
     1261 tgtatgtttc caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg
     1381 gattttcaac agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta
     1501 tgaattttgt gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga
     1621 agattgtcat gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata
     1681 gcctgttttc catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca
     1741 ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca
     1801 atggcgaaga cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc
     1861 taaccattac aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag
     2401 gacagtcggg ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa
     2461 aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca
     2521 aagacagaat ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca
     2581 atgcaagata tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa
     2641 ccagtagtat ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac
     2701 aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
     2761 agcctgcttc ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac
     2821 agacctcaaa acagtctaat attcagactt catataagga atctcaacaa gccaaacaag
     2881 agcgtccagc agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg
     2941 ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac
     3001 aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa
     3061 gaagtagacc accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt
     3121 gaaaacttta gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt
     3181 tgttgccatt ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg
     3241 tgtgaatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga
     3301 atatggcgtt gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg
     3361 tactgcggaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
     3421 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga
     3481 aaggctcagt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
     3541 agggtatgtg gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa
     3601 agagtattca ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
     3661 gggctggcga tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
     3721 cacaacagag tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga
     3781 gggctggcga tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact
     3841 gacacaatgg acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata
     3901 tgatgtgacc cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca
     3961 ttctacctat aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg
     4021 tatgtttcat gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca
     4081 gcatttagtg ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt
     4141 agaaaaaatc agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag
     4201 gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc
     4261 tttgcgatat tcaagcactt tacagctata gatactcata ctattcacga aacaactatc
     4321 atagaaaagg aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa
     4381 gtctactatt catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa
     4441 ggctatctga cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct
     4501 gtatcgtctt ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag
     4561 tttaatgtaa cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc
     4621 cactctgctt atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag
     4681 aatccgacca ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt
     4741 gaggggacgg ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc
     4801 aagctctatc ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta
     4861 aaaccaatcg gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag
     4921 gataatcaag tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg
     4981 caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa
     5041 taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa
     5101 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag
     5161 gagttaataa atatgcggca aggtattatt aaataaactg tcaatttgat agcgggaaca
     5221 aataattgga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
     5281 ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat
     5341 gcataaaaat cccagtgata agagtattta tcactgggat ttttatgccc ttttgggctt
     5401 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
     5461 aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
     5521 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
     5581 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
     5641 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
     5701 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
     5761 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
     5821 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
     5881 caaacagaag gtagaactgt atcctaatgt gtgtgtgacg aactttaccg aatctgaaca
     5941 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
     6001 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt
     6061 ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat
     6121 tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt
     6181 caaaattgaa tatacaaaaa aaa
//
LOCUS       DADXTG010000127         6168 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.129, whole genome shotgun sequence.
ACCESSION   DADXTG010000127 DADXTG010000000
VERSION     DADXTG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.129"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(129..776)
                     /locus_tag="IWI57_002299"
     CDS             complement(129..776)
                     /locus_tag="IWI57_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBC2450405.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1184..1687)
                     /locus_tag="IWI57_002300"
     CDS             complement(1184..1687)
                     /locus_tag="IWI57_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2450406.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1810..2574)
                     /locus_tag="IWI57_002301"
     CDS             complement(1810..2574)
                     /locus_tag="IWI57_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBC2450407.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2682..2957
                     /locus_tag="IWI57_002302"
     CDS             2682..2957
                     /locus_tag="IWI57_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBC2450408.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(3158..3505)
                     /locus_tag="IWI57_002303"
     CDS             complement(3158..3505)
                     /locus_tag="IWI57_002303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450409.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            3673..4602
                     /gene="yidC"
                     /locus_tag="IWI57_002304"
     CDS             3673..4602
                     /gene="yidC"
                     /locus_tag="IWI57_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBC2450410.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            4705..6053
                     /locus_tag="IWI57_002305"
                     /pseudo
     CDS             4705..6053
                     /locus_tag="IWI57_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1924 a         1178 c         1131 g         1935 t
ORIGIN      
        1 aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg aaacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtga tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgttctactt ctcttattat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ctggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgcttatt attctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa agtcgtgtca
     1021 aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcagtaa taagccgaaa
     1081 cttcacaaaa atttgagaag taattttcgt aaattccttc ttatttcttg aagctgacca
     1141 cttctgtcac aacctcaata tggtctatta ttggttttac ctgttaaatc gaggattctt
     1201 tcccaaagta acgttgccat ctcttacgca tacttgcaga catgggctga gccgcttctt
     1261 tgactgcgca gtatttgtcc acgaatgtca caatatatcc ttctttatac ttaggcggtg
     1321 cgatcgttgc accccacatg tgcttcaaaa tgatatctcg ctcaagatcc gacaaatcgg
     1381 tgattttttc tgcattcttt acagcaattc ttggatggat atacgcatgt gtcccctcgt
     1441 caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca gcacgcgctg
     1501 ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa gaaacactga
     1561 tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat tttttcactg
     1621 cttctgtttc taaaagatct tgtacataag acatatattc ttcgtcttca cgccattttt
     1681 ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa attgatctaa
     1741 gtataccacc agtgttcgga ttattctagt cgctctagat aaaatagcta aagcttaata
     1801 gttccttaat cataaagaaa aatgataaag aacgatccct gctgcgactc cgacattcaa
     1861 tgattccgca tttcctttca ttgggatata tacgttctga tcagtcaaag acaagatttc
     1921 ctgactgact ccctgacctt cgtttcccat gattatagcg aaatcctctg ttttttctac
     1981 tttgtctaat gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa accctttgcc
     2041 tttcattaca gggattacat ctgccaaatc tttttgtaac acaggcaaat gataattgct
     2101 tccctgcatg cttcgcagca cctttgtact ataaatatct gccgttcctt tacctaataa
     2161 aacaccagcc ataccaaacg catctgccgt acggatcatc gttcccacat ttccaggatc
     2221 ctgtacatta tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt cagatgttaa
     2281 cgtcatcact gctaagatcc cttgcggtgt aggtagatct gacatagcgg tcatgacctc
     2341 atctgaaact agaaaagtag aaatctcttt gtttacagcc agccagtctc cccattcttt
     2401 catacctctc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg cagcttcttc
     2461 gatcagatgg aacccttcca aaagatattc tgccttttct tctctgtatt tctttttatg
     2521 tagttttttt aattctttga ttcttggatt ttttgctgat tggatctctt tcacattcct
     2581 tcacctcacc tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc
     2641 ggtatcattg atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga
     2701 atgtccaagg acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc
     2761 agttaggcgt gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa
     2821 tcggaaatga cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa
     2881 gtggacgagt gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat
     2941 ttgatgtggt gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt
     3001 tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt
     3061 tgaacaccgt cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat
     3121 tttcaaaata gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata
     3181 aaacaccgtc aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt
     3241 gaaaaagtcg cttagttttg agtattaagg aaaattttgt gaaaatggct ttccacattt
     3301 ttgcataaat ttgtcttaat accaagaaac tgacttttga acaccgtcga taaggttgtt
     3361 tttgaggcgc tttgccccaa gtattaaaga caggtagtaa aaaagctgat ctatcacttg
     3421 cagtgagttc atttgactcc tctatttcgg gcagaataaa aaaagttttt agttttttaa
     3481 ataagaaaga aacacttgga atcactcttt tctttccaag tatttctttc ttgatttttt
     3541 gtcttttttc aaaatttttc gctacgaaaa agaaagaata tttatatgaa atttctatga
     3601 agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag
     3661 aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc
     3721 ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcagga
     3781 atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat
     3841 ttcaattggt cttacggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc
     3901 ttgccactag gtatcagtca atcaaaaaaa acaatgatcc aatctgaaaa aatgcaagcg
     3961 ttaaaaccac aagttgaagc agcacaacaa aaactaaaag ctgcaacaac acgtgaagaa
     4021 caaatggcag cacaagcaga aatgcaacaa gtatatcgcg aaaacggatt gagtatgacc
     4081 ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtatttt
     4141 acggctcgct acacagaagg tatccgtgaa tcatcttttt acggcatcga tctaggctca
     4201 ccaagtatgg tgcttgttgc cattgcaggg gtcgcttatc tgcttcaagg atatatttca
     4261 acaatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct
     4321 gctatgatcg ttttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtg
     4381 ggtggtgtgt tcagttgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg
     4441 aaagcacaag tggcagaaga gttgaaacaa aatccgccaa aacaagtcgt cactccaaga
     4501 aaagacgtga cagaatcggc aaagccagct gacaataaac aaccaaaaaa cagtacaaac
     4561 aacaaaggcc gcaacgcagg aaagcagcaa aagcggaaat aaaaatatat gacaaaaaac
     4621 tgtagacaaa taaaaaaatt tctttgttct acagtttttt tgttacaatc aaagaaaacg
     4681 tcgcttttcg aggaggaatg tataatgatg tccaaataat caatggatag tcgaatggaa
     4741 gaagccaaaa tttcgctaga tgcacttgtt cctaaaaatc atttggtcca taaaattgac
     4801 aaaatattgt tatttgattt tatttatcca attgttgaat caacttactc gaccattggt
     4861 cgtcctagcg ttgatcctgt catcttagta aaattagtat tcattcaata tttatttagg
     4921 attcgttcga tgcgtcagtc cattaaagaa gctgaaacaa acctagctta tcgttggttt
     4981 cttgggctta gtttaatcga taaaattcct catttttcaa cgtttggtaa aaactatgta
     5041 cggcatttca gagaaaccga ttgtttgaac agatatttat cattatcctt gaacaagcag
     5101 ttgaagctag atttattcat gaagaaattt tgtatatgga ttcaacctat attaaagcga
     5161 atgcgaaaaa acatattttt acaagagaaa tggtacataa ataagccatt ttataccaaa
     5221 aacaattgaa agaaaaaatt aatgaagcaa gaattgcaga aggcaaacgt ccttttggtt
     5281 caatcattca tcaggaggtg gtagaaggga aaattagtgt cgcagatcca gaaagtggtt
     5341 attatgtaaa aacagaacaa gaaaaacagt ttgcttattc tgctcatacc gtttgtgatg
     5401 aaaatggttt tgtattagat gtcatgatta cgcctggaaa tctacatgac agccgaatgc
     5461 tcgtgccaca aatcgaacgt gttaaatcat gttgtggtgt agctgctgat gctgcgtaca
     5521 aaacaccttg gaacgccaag tatttaatcg atcgaaaact acgacccatt tttccttata
     5581 ctcgacccaa aagaagcaaa gaacgtttta aaaagaaaga tttttattat gatccctatt
     5641 ataactggta cctatgccca aatgatcaaa cacttcagtt tcgcacaact actcgagatg
     5701 gttataaaaa atatgtatct aaatctttta tttgtgaaag ttgtctatta ttgaaaaatt
     5761 atacagaaag ccagaaacac cagaaagaaa tacatcgaca tatttaggaa gactacttag
     5821 atgaagcaga acatttacgc catacggtgt acaatcgcga aaaatataaa aaaaagaaaa
     5881 gaaacgattg aacgcgtttt tacagacgca aaggaaaagc atggaatgcg ctacaccaaa
     5941 tatcgaggct tggaaaaaac caggtgcata cgatgcttgt ttttgccgca atgaatctgc
     6001 aaaaattagc catttggcag tgggggcata tgtctttata cccgtggtgc tagttaattt
     6061 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atatagtagt
     6121 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatat
//
LOCUS       DADXTG010000128         6090 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.130, whole genome shotgun sequence.
ACCESSION   DADXTG010000128 DADXTG010000000
VERSION     DADXTG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.130"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(236..523)
                     /locus_tag="IWI57_002306"
     CDS             complement(236..523)
                     /locus_tag="IWI57_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBC2450411.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(543..767)
                     /locus_tag="IWI57_002307"
     CDS             complement(543..767)
                     /locus_tag="IWI57_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBC2450412.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1222..1437)
                     /locus_tag="IWI57_002308"
     CDS             complement(1222..1437)
                     /locus_tag="IWI57_002308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450413.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(1407..1940)
                     /locus_tag="IWI57_002309"
     CDS             complement(1407..1940)
                     /locus_tag="IWI57_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBC2450414.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1961..2905)
                     /locus_tag="IWI57_002310"
     CDS             complement(1961..2905)
                     /locus_tag="IWI57_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBC2450415.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3542..3628
                     /locus_tag="IWI57_002311"
     CDS             3542..3628
                     /locus_tag="IWI57_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBC2450416.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3749..4438)
                     /locus_tag="IWI57_002312"
     CDS             complement(3749..4438)
                     /locus_tag="IWI57_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450417.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4445..4723)
                     /locus_tag="IWI57_002313"
     CDS             complement(4445..4723)
                     /locus_tag="IWI57_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450418.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4683..5597)
                     /locus_tag="IWI57_002314"
     CDS             complement(4683..5597)
                     /locus_tag="IWI57_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBC2450419.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5579..5959)
                     /locus_tag="IWI57_002315"
     CDS             complement(5579..5959)
                     /locus_tag="IWI57_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBC2450420.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1616 a         1116 c         1029 g         2329 t
ORIGIN      
        1 caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt tcgcttgttt caccttcggt
       61 gaaattgttg caaatcaata attaggtaga gctacatttt tttcgttgtt caaagattct
      121 tatgccattt actattaata aatctataaa tatgataatt tttcggctct ataatttatg
      181 ggactgatga atcagaattg attcttcagt cccattttca ttttattcca aagaattaac
      241 tccaaatacc aatagaagcc catcctctat atacttgaga ttccttacta acaatttctt
      301 gtaattctat taaagattta ggtaatccaa atttatgttt taaaatatat cttccaagaa
      361 aactacttaa tttcatacta acttcaccaa cactttttcc ctcactgatt tctttagaat
      421 aaagaaagag cttttctttt aagtcaggat agtcatctat agttgcttca ttgtatactt
      481 tactaattgc atttaaaagt ttttcttctt tagtaaaatc cattataata acctccctac
      541 cactaccttc taggtgccca tggaccatga ttaacccaac cattaacaat aatttttcca
      601 atttctcctt tagcttgatt ccaatctacc caacattttt ctttgttaca ataaagacca
      661 tttccatagt aagtagctgc atcaactttt attcctgtac cgatgccagc tagacaagtt
      721 cctaatattc ctagaataac acaatgtttt aatactttct ttttcatgat tgtttttcct
      781 tttcgttttt ttaaaattgc ttgttatatt aaataatctt agtaatttta ttatataaca
      841 ttttattata taacaatgat taacgtcata aaaagtagtt ttgaattcat aatcgtttaa
      901 ataaaattat aagatcagtt atttaaacag ctagtagaca taatgtcgat actaccaagt
      961 aacgcctgta ccaagggatc caggcgctga ttttgtgaat ttttattggt ctaggttaaa
     1021 agctcttttt aaggcttggt tttctgcggt tgtttctttc taattctttg tgaatattga
     1081 tcggtcattt tttaaatact tgttattttt gtttgtgttt gttatgcttt ttacatatct
     1141 catattcatt tattattctt ttctaaggtt aataatagga taatctcttt cagaaagact
     1201 gctcgtacga gcagtctttt cttatttatc aagtttttct acaaatactt cctcattttg
     1261 actgttgtag taattaggat tgagttttag ttcccctttt ttatttcctt tgttggccac
     1321 tgataataac attgggctaa agcttaatat tcctagtagg atagaaccga atagcaagtg
     1381 tttttttgtc ataagtattt ccttcttcat ttccatagct gccaccattt tttccaattc
     1441 tttgtttgtt cgtccattgt ttctgttgct tcagaaagag tcgttgaact attctctgaa
     1501 gactcttccg tataagtcaa ctgcagttgc tcctgaagtt tttcgatttg ttggttggct
     1561 tgcagggtga gttgttgttg ctggtcgagt agtttttgta tatgagaatt attttgctct
     1621 ttgagggcaa gttgttctcg taaaaattca ttttcttcac gcaactgatc tagcaacttg
     1681 tttgcaacca cgtttttgtt tggtatataa gcatttttat cgttgttttg ttcatgttta
     1741 tgcaacttat ctagcaacct ttgattgtct aagttgcgag caagttgcaa tactccagaa
     1801 cgcaatacaa ccttataacc acgctcattt ttagctatca tggaagaatc taatttttgg
     1861 atatgtttcg atattgcttg cctactcatc ttaaaaagat ctgacaattc ctttattgta
     1921 aaggtttctt gtggttgcat actggttgcc tcctaggttg ctactggaat aaattatcta
     1981 aatcagataa taaacttttt ttctcttttt ccgaaagtgc ggactctgtt tgtttttcaa
     2041 gagctttttt ctctttttct agttgtttga tttgttggag gagttcaagt tctcgtttct
     2101 tctgtttgat tttttcgtta attgttgtag cgggttcttc gtattctttt tgtttatcag
     2161 ctaaagccaa tgctttattt tggtcttctt tggttggtgc atgtttgtta aagtcgtctg
     2221 cttttttgat ctcaggtttg aacgtaaatt gatagccaat aacaggtttt ccccgtcctt
     2281 ttccgtattt ctttttgatt gttagtcctt tgaagattgc agttagttct tgacgtatag
     2341 gttttataac tcgtttatct atatcaggtt gcttataact tttaggaata tctaataatt
     2401 caagaaagtc ttctttggag aaatatgcga aacctttggt tctgtattgc tttaacagac
     2461 gaaacatagt ctttgcatag ccactttgta gctcgttgaa ttgttgtaaa gaatagcgta
     2521 cccactcgtc taaattattt aacaaaggca gtgctttttc gtaaattcgt atatctacgt
     2581 aaggattttc tacgtccccg ttgattttaa attctgtaaa cattacaaac atatcgtaag
     2641 aaagcccaga ctcacttcta cgaccaaagc gtaaagataa gattttttca taagttgctt
     2701 ttaaatcttc tacaaatcgt acatttgcag ttgctttgta gttacttaaa tcttttagtt
     2761 ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg cattctagac acgatggaaa
     2821 aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg aattgtattt aattcatggt
     2881 ggtattttac aatctcatta gccatataat cacctcaatc caattatgaa tgacattttt
     2941 taaatgtcaa gacattcata tttgacgttc aaacagacaa aatgtccttc ataaacagac
     3001 aaaatgtcat tcataaacag acaaaatgtc attcataaac agacaaaatg tccttcataa
     3061 cattctgtat ccattggtag agtaaggaca cagcttacgg taaagaggtt taaaaagagg
     3121 tttaaaaaga gatataaaaa gaggggggct aattttcccg cctaaaaacc acttcttttt
     3181 tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc aaaaccttgc ttcctacggt
     3241 cgccaaaaag gtgtgatcgc tacgctctca cattgtactg cgcagtcgcc taaaggctcc
     3301 tttgctaaaa acatgttggt aaagaggcct ttgagcggtt ctgaatgctt atctaagccg
     3361 ttttaagaca gtcttggata aaaatcctta tctagcttta aagcgcctta caggcggtca
     3421 gggcaagccc taacaaccct aaaataagcg atacttaaaa acaccacaca agtttaattt
     3481 tttaagatat aataacttgt cgcctatccg acagtcttgc gactggaagg aggtgtatct
     3541 aatgtttgaa ttgttcattt gcccccttct tatagaagtt tgtgtctctt tattcagtta
     3601 ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg cgacatcaac ctgaaaaaaa
     3661 gccccagaga atccgacctc tctggggctt tcgtgtatct aatgtttgat catttgcatg
     3721 tttagtttaa catgattgcc gatcaaattc aattttgctg ttgctgatct aaccattcct
     3781 tgacactttt tagataattt aatgccgttt cttttttcgt tggattttgt atgttcgttg
     3841 cttgctgaag cagtgcttta taacgttcgt aggtaactga ttgttgccgt tcttttcgtt
     3901 cctgttcact agttgaaaaa gggattgtat cactgaataa attataacaa gcttcttctg
     3961 tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg ctcatagttg tcttgaacct
     4021 ctttttgctg tttttctggc aagttttgat aaagacgagt gagtttttcc atgtttttat
     4081 ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat ttcctcataa caagtggtaa
     4141 actcttccat tttttgtgtt aacacttgct cctttttctt tttatcacat gctgcttcat
     4201 acttttgagt gatatcccct aataaaatac ttcttgaaat acgtttattt ccctctaaaa
     4261 cgccgaataa accacttaaa acgattgata atgtaaatat aacccctggc aataaaaacc
     4321 tcttaaatcg aattttagag ccttttaatg aatatcttgt ttgataagcc ttatagctaa
     4381 gccaaataat tcctaaaata ctggcaaaaa tgagtagata gggtaaatag aatgccattt
     4441 ctatttattc tccccatacc agccaaaagg ctaacagtgt catgattaag caacataagg
     4501 gggcaagaat atccattgat cgctgcataa ttactttttt ctgttcttta tttcgctggt
     4561 ttaatagcgc atggtggaat tttttgactt gtgaatcaat tgtagtttgt attctttctt
     4621 tggtataagg tgcaagaaaa gcacgttgtt tctttatttc attttctatg tcttgtaacg
     4681 ttttagaacg ataattcttg ttggtagtct gctcctcttt caacgtgttg gtcatggttt
     4741 cttcgagtcg gtctaatttc cggttgttca gtcgtaagag ttcgttgttc tcttcgtact
     4801 tcttttgcag tttcgcgaat gctagctcga gctcttcgta gctcgttggt tcgttgttga
     4861 ttgtaggctc gttctcgtct gtccagctca tgtaaaatcg tctccttttc gtaatccttt
     4921 cctagtttgt tggatcgtac ttttttcttt ttgtggatca attcgtaagt aaaatttttc
     4981 ccacgattca agcaatgaat gccgaatgtt ttaagagaat cacaaaattg gttatacgtt
     5041 cgagtttttt tgtttttcat tgtttgatca atcgcaaacc gaatttcgtc tttccaaaca
     5101 ggttcatttt tttcttttaa tttgatttca gacatggttc gtttttcggg tttcttccct
     5161 cgttcagtga ttgttaagtc atgttcaaga caaatctcgt catttaggtc ataacatttg
     5221 tttaaaaatg cttgaccatg ggcttgaaat ttgttcccag tttccagatt gacggagtta
     5281 atgacaatgt ggttatgaat atggtctttg tctgtatgtg tgtaaatcgc gatttggtgg
     5341 tcaggggcta tttttttcgc atactctgcc cctagttgat tggctttttc agcggtaatt
     5401 tctcctggtc gaaaagactg gatcaataag tgtgcttgta cacgatcgtc ttttccatac
     5461 agcatgcgaa tttgtttcat ttcgcttttg gcataatcaa tgtcacagtt tatccctgtt
     5521 ttgacggttg ctcgtggttc ggcgtaattg atcgctcggc tacaagaagt ggttcggctt
     5581 actttaactg ttgccatatc ttttgtagct ccttttgcat gatttttagg ttttcggata
     5641 agcgttgggc gtcgccagga tcaacgtcgt gttggtgtgt gttgcaccac ttggcaattt
     5701 gattgatatt cccgccagct ttggctaaat cgcgcgcgat tgcctgagca atgtctggcg
     5761 ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc tttcacgaaa gccggcacac
     5821 tcatttgcaa agcttcagct gactgcttca gctttgcaaa ttcggattcg ctcactcgaa
     5881 aactgatttg ttttggctcg gatcgatagg tatatttttt agaatgattg ctagccaaat
     5941 attgattttg ttcgctcata agtcccaccc tttcagtacg tcctacggac gtatcgttcg
     6001 caataccatt gtacctaaaa aggtacaata agtaaagctc actaaagctt actaaagggc
     6061 ttggcaagct ctatatttgc tagccaaata
//
LOCUS       DADXTG010000129         6074 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.131, whole genome shotgun sequence.
ACCESSION   DADXTG010000129 DADXTG010000000
VERSION     DADXTG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.131"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            130..1869
                     /gene="yfcC"
                     /locus_tag="IWI57_002316"
     CDS             130..1869
                     /gene="yfcC"
                     /locus_tag="IWI57_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBC2450421.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2026..3210
                     /gene="iadA"
                     /locus_tag="IWI57_002317"
     CDS             2026..3210
                     /gene="iadA"
                     /locus_tag="IWI57_002317"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBC2450422.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3321..3524
                     /locus_tag="IWI57_002318"
     CDS             3321..3524
                     /locus_tag="IWI57_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBC2450423.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3625..4287)
                     /locus_tag="IWI57_002319"
     CDS             complement(3625..4287)
                     /locus_tag="IWI57_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450424.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4300..5802)
                     /locus_tag="IWI57_002320"
     CDS             complement(4300..5802)
                     /locus_tag="IWI57_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBC2450425.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
BASE COUNT         1832 a         1104 c         1292 g         1846 t
ORIGIN      
        1 aaacaaaata gaaaaaagtg attttctaat aaattattca tgtggaaatg tcatttttac
       61 tgtataattc atttgattat ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa
      121 gaaataaatt tgaagaaaac aaattcttca ggaaaaccga aacgcgactt cactaaatta
      181 tcgacacccc atacgtatgt cattattttt ggtgtagtca tctttgcatg gatactgact
      241 tttgtcgttc cagcaggaaa gttcagcacg caagatattg aatacaaaga tgcaaatggc
      301 gaaacaagta ctcgtactgt tttacgtcaa gattccttcc gttatgctta cgagctggat
      361 aagtcgtatg tgtttgatca attagaagaa ctgcaagatc atccagcaga acgtgaaaaa
      421 ctggatgttc cggaaaaagg attggaaaaa gtcattgctg acggagaaaa aaatcttacg
      481 caggaaaaac tagatgaaat ctccttgaca gatgatgtgc tttacgatca gtacggcgaa
      541 aatatctatg atacttctaa aaaattgcat aaaacagcca aaatatgggg aacagatgat
      601 tttggcggct ttggtttctt aaattttgtt tttgaaggac tagtatctgg agataagtat
      661 ggatctgctg tagggatcgc tgcattgatc cttgttgttg gaggtgcttt tggtattatt
      721 atgcgtaccg gagcgatcga cgcaggtatc tatgcgttta tcagtaaaac taagggactg
      781 gagcgtttag ctttaccgct attattcttt gctttttcat tcggtggcgc tacattcggg
      841 atggcagaag aggtaattcc attctcaatg gtgatggttc catttgtcat tgcgttagga
      901 tatgactcga tcgtagcagt aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc
      961 tcttggatga gcccgtttag cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg
     1021 tctggtgcga cattccgatt gattatgtgg gtagttgtta ctgcattagc cgctggttat
     1081 atgatgatat atgcagaaaa aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca
     1141 gatgattatt tccgttctca tatcaaaaag acatctgatg aaaataaaaa attcatgctt
     1201 gggcataaat tgatcttatt agagatgtta gttgtccttg tctggatcgt ttggggcgtg
     1261 actcaaaaag gctattacat tccagaaatc gcttctcaat tctttgtgat gggattagtt
     1321 gctggaatca ttgctgttct tttcaaatta gatggaatga aagtcaatga catcgcttct
     1381 tctttccaat ctggtgcagc tgatttagct ggtacagcaa tcgtagtagg tatggccaaa
     1441 gggattcttc ttgtcttggg aggatctgat gcatctgttc cttcagcttt gaatacgatt
     1501 ttgcatggga tcgggacggc gttgaccggt gttccagctg tcattggtgc ttgggcgatg
     1561 tacatcttcc aaagtttgtt caacttggtt gttacatcaa attcaggaca agcagctttg
     1621 actatgccaa tcatggctcc actggcagat ttagtaggtg tatccagaca agtagcagtt
     1681 cttgcttatc agcttggttc aggttttatg gacgctttta cacccgtatc agccagcttg
     1741 attggtgtgc taggtgttgc acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag
     1801 atgcaaggat tcctgtttgt tttaggaact attttcatca tgattgccat tgcaataggt
     1861 ctgcagtagt actggagacc tgtaaataaa aaagtaatcc aaacagtaga taaggactcc
     1921 aagaaccgtc attttacggt aaaatcaaag gagtaaagat cctatcgttt ggattatttt
     1981 ttaagatcct atcatcagtt atacttaaag aaagaggaaa aatgtatgaa aatcattcga
     2041 caaattgaag tgtttgcacc agattactta ggaaaaatgg atgtactgat tgccggtggg
     2101 aagattttgg ctgtagaaga acagctagaa ggcggctacg aaggagtgga agtagaagaa
     2161 ctttcaggtg agggaaaagt actaaccccg ggtttcattg actgtcattt ccatatttta
     2221 ggtggaggag gagaaggcgg ttatcaaaat cgaacgccag aagtcacatt gagccaattg
     2281 actacagctg gtgtaacgac agttgtcggt tgtttgggaa cagacggaga aggtagagat
     2341 atgactgcgt tgatcagcaa agcgaaaggt ctggaagcag aaggaatctc cacttatgta
     2401 tacgaaggtt cgtaccgatt gccggtaaaa acagtaacag attctatcat caaagacttt
     2461 ttgacaattg ataaaattat cggaatcgga gaaatcgcag tatccgatca ccgttcttct
     2521 cagccaagtt ttgaagagtt cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta
     2581 tccggaaaag ctgggatcat caatgtccac ttaggcggag gaaaaagaaa gctagagcta
     2641 ttgacacgaa tcgtagaaga aacagaaatt ccgatcacac aatttttgcc tactcatgca
     2701 aatcgcacac cagaattatt tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt
     2761 gacttcactg ctagcgaaga tcctgatttt tgggagaaga cagatggtga agttcgcttc
     2821 agcaaagcac tgaaacgatt gatcgaggaa gatgtttctt tagataattt cacgatgacc
     2881 tcagatggac aaggaagctt gccttatttt gatgaaaaca atcatttcct agggttagga
     2941 gtaggaagtg ccaaggcatt gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc
     3001 cctttggaaa tcgccttacg agctataacc agtaatccag cacgtatatt gaaattggat
     3061 aaaaaaggaa aaatcgaaat tggtgctgat gcagatctgt gtatcttgga taaagaaacg
     3121 cttgatattg atacagttat cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa
     3181 gtatggggga ctttcgaaaa aagtttttaa tcaacagctc taactatctt ttttagaaaa
     3241 cgagcgttct gtttgtctgt atctgttgtt gatgttaccc tgaaggtaga tcaataaaca
     3301 aactaagagg aggaagcaca atggcagatt taaaaggacg ttctaaagat atgaaagata
     3361 aagtagtcgg tgaaggaaaa gaagcatacg gcaaagcaac agacgacaag agcaaagaaa
     3421 cagaagggaa agcacaatca ctaggtgctg acatcaaaga aaaagctcga aatttaggcg
     3481 acgaagtcaa agacaagttt tctcgaaaag acgaaaaaaa ataaaaacca cttattacta
     3541 attgaatcaa gcaaataaaa aatccgaacc atatctgcga gacagatatg gttcggattt
     3601 tttatttgat cattatcctg ttttttaaac ttcgattttt tgaaattttt tattttttag
     3661 ttcaaagact tcatcacagc tttttgctac ttcaatctca tgagtcacga tgataataca
     3721 gcgattttgt tcatgggcga tttcttggaa aagtttgacg atatcttgtg aagttgtttc
     3781 atctagattt ccagtaggct catccgctac gattagttga tgttcacagc agatagcacg
     3841 aacgatagcg actcgctgct gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa
     3901 tatttcatca attcctacac gagccagtcg atctaaagca acttgtcgat aattctcttg
     3961 cttggtctta gtgatttcca ttgctgctat gacgttgtct agagccgata aataaggaag
     4021 caagttgtaa gcttggaaaa taatcgagac atcattttta cgataacttc ttaacccgat
     4081 tttgttcagt gaacgatctt gatagtatac ctttccttct tttggtgcat ctagtccaga
     4141 aataagcgaa agaaaagtcg tttttccaga accacttgct cctaaaatag cgtacatttt
     4201 tccagcttcg aaattcagtt ctacatcttt ataaagcgca tcttgcggat cttgatacca
     4261 aaatcccaat tgttctgtcc gtaacatcaa attcctcctt tagtttaata ggatttttct
     4321 tgggtttaat cttaatatcc ctatagaagc taaaaagaca gaaaacagac tgattccaaa
     4381 tgctactccg gctaatagtc ccaattgtgc aggtttgaca ttgatttcca attcgctgac
     4441 ttgatcagaa acagcaaatg gattattcgt ggttccttgt tgaccatcgc gttgaccgcc
     4501 ttgttcttgt ctgttacctg gcatttgtcc accgccagga gcttgctggt tttgtgtcgt
     4561 ggtttctgtc tgttggtcaa gtagctgctg tcccacggca ttcgcgacga cgtttccgct
     4621 aagcgaagca actcctaatg caagaatcat acaaatagcc acttcggtaa acaattggag
     4681 aatgatttta cttctggatt ctcctaaaga tagcaagacg cctaactcat gccgacgctc
     4741 ccgaatggta atcataatga tcagagtaag tatgataata ccggctacag ccactagtaa
     4801 aacaacattt ttagaaaaac ttgccacgtt gtttaaaggt tccaacattg actgatacat
     4861 ggaatcattt gattgtaagc taaatgtatc tgtgtcgatc agcttctcag cttctttgag
     4921 aaactcatcc attttatctg gatcagatag agtgtaaata gcagaatcta tcgtgttatc
     4981 gtcagatgtc ccatttagtt catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg
     5041 attcatgaag ttgaatttca tacccatact gccgcttgtt tcgctggatt cataaattcc
     5101 tttgatcgtc atttcataag tggtatcttc atcttcagta ctcgtgattg tgaaagtatc
     5161 tccgactgcc aggtcatttg tttctgctag agtagaatca atgactacat tgtttgtgcc
     5221 tttgtcgctg tcatctattc cttcaccatc cgtgattttt gctgttccat cagaaaaatt
     5281 actattttgt gcagttgctg acacaccact gatttgaaag tctgcttgag tcatttgcgg
     5341 attcccgccc attcctccag caggttcacc gcctgcatca gtagtttctg aactgtcttc
     5401 acttgaatca gaactagtga tggcagttat tccatctttt gccatagctg aggcggaact
     5461 ttcaaaggaa taactcttta cattatctaa ttcagctatt ttttccgcgt ccgttaagga
     5521 aactggtgta agagaaaaac tgcctggatc tggacgttct tcagtggtag aaccgcttga
     5581 agaagtttgt tcctgttttt taaatgaagc ttctcgattg gcacttaaag tcactgttgc
     5641 tccaacttct ttttgtgctt gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc
     5701 agctagcaca aaaataagaa tggctgtgaa cacggcaaac aataacaatg aacgtcccca
     5761 ttttactttt gtacttttcc atgcacgttt cataaaattc atattttgca tccctcctag
     5821 tagatgcatt tgataataga caacctatct taaagctagc ttaaatctta tgctgtttgc
     5881 acaccatcaa catgaggttc tgtatatata aaaagtcact atagttattg caaaaaatgt
     5941 ggtaaaatga aaaaagtatg tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa
     6001 agaaatccta gatgctacag agggattttt acgtgcatag ttgttctaac aattgctgat
     6061 actagcgatt tagt
//
LOCUS       DADXTG010000130         5939 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.133, whole genome shotgun sequence.
ACCESSION   DADXTG010000130 DADXTG010000000
VERSION     DADXTG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.133"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            361..642
                     /locus_tag="IWI57_002321"
     CDS             361..642
                     /locus_tag="IWI57_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450426.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="IWI57_002322"
     CDS             644..1060
                     /locus_tag="IWI57_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450427.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="IWI57_002323"
     CDS             1682..2836
                     /locus_tag="IWI57_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBC2450428.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="IWI57_002324"
     CDS             2830..3252
                     /locus_tag="IWI57_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450429.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="IWI57_002325"
     CDS             4422..5390
                     /locus_tag="IWI57_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBC2450430.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="IWI57_002326"
     CDS             5407..5931
                     /locus_tag="IWI57_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBC2450431.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT         2253 a         1015 c          963 g         1708 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagca
//
LOCUS       DADXTG010000131         5910 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.134, whole genome shotgun sequence.
ACCESSION   DADXTG010000131 DADXTG010000000
VERSION     DADXTG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.134"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IWI57_002327"
     CDS             complement(368..1486)
                     /locus_tag="IWI57_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450432.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IWI57_002328"
     CDS             1957..3264
                     /locus_tag="IWI57_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBC2450433.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IWI57_002329"
     CDS             3383..4582
                     /locus_tag="IWI57_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBC2450434.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IWI57_002330"
     CDS             complement(4609..5877)
                     /locus_tag="IWI57_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBC2450435.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1814 a         1252 c         1058 g         1786 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact
//
LOCUS       DADXTG010000132         5906 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.135, whole genome shotgun sequence.
ACCESSION   DADXTG010000132 DADXTG010000000
VERSION     DADXTG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.135"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(119..604)
                     /locus_tag="IWI57_002331"
     CDS             complement(119..604)
                     /locus_tag="IWI57_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450436.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(743..988)
                     /locus_tag="IWI57_002332"
     CDS             complement(743..988)
                     /locus_tag="IWI57_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBC2450437.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1181..1981
                     /locus_tag="IWI57_002333"
     CDS             1181..1981
                     /locus_tag="IWI57_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2450438.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2000..3868
                     /locus_tag="IWI57_002334"
     CDS             2000..3868
                     /locus_tag="IWI57_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBC2450439.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3861..4352
                     /locus_tag="IWI57_002335"
     CDS             3861..4352
                     /locus_tag="IWI57_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBC2450440.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4340..4894
                     /gene="srlA"
                     /locus_tag="IWI57_002336"
     CDS             4340..4894
                     /gene="srlA"
                     /locus_tag="IWI57_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBC2450441.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4912..>5906
                     /locus_tag="IWI57_002337"
     CDS             4912..>5906
                     /locus_tag="IWI57_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBC2450442.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     EKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2061 a          945 c         1193 g         1707 t
ORIGIN      
        1 agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat aactttctca
       61 gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata tcatctaatc
      121 aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac actcgataaa
      181 aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct gtgaacaaat
      241 aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt aattcggaac
      301 ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga gaaggtacag
      361 tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt gattggctat
      421 gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa gtacttttag
      481 ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa ttctttgttt
      541 tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa atccctttat
      601 gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta aacaaagaaa
      661 agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg aagataataa
      721 cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta atcattggga
      781 aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa
      841 tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag cctaccaaca
      901 gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag ttttcattag
      961 aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca ttgcttatat
     1021 aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg ctcttccatt
     1081 agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt agtgagcttt
     1141 tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt ggttggatat
     1201 tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa gtatcgtcga
     1261 aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat gccgtataca
     1321 gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca tagaagaagg
     1381 gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata atgcaggaat
     1441 aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg aattatcaga
     1501 acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga tgagccaagc
     1561 agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt cttctgaatc
     1621 aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag cgatgaacag
     1681 ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg taggtgttgc
     1741 accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag ctctttctta
     1801 tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa gtacgatccc
     1861 tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt atctttcaga
     1921 aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa caagaggata
     1981 aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat tgaaattact
     2041 agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa ccagtaatca
     2101 aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg caaaaatcat
     2161 ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg atgagatcat
     2221 gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa tcgcttattt
     2281 actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt ctgaagaagt
     2341 cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga tgatcaatga
     2401 ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag gagatgagct
     2461 agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga cgaaaacaga
     2521 acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga atgctagtaa
     2581 acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa tctctagtaa
     2641 ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata tccaagaatt
     2701 caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc aatatgaaaa
     2761 agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga taaggaaagc
     2821 taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac gattagatct
     2881 cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct ctttattgat
     2941 aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga tagaaaaaaa
     3001 gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag ctatcttgca
     3061 acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt tagcatttcg
     3121 atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga caggaaaagg
     3181 aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata tacaaataac
     3241 acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg ctgtttttac
     3301 aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga ctaatctttt
     3361 tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat tagccgggtt
     3421 tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc aagaatattt
     3481 aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat ttgaaaaaag
     3541 gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg cttttcctca
     3601 cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa ctgatcaagg
     3661 tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg aagaaatgac
     3721 ggctgaagtt gagaatgaat tatttgagct atgcgatatc ctatttgaac tcataaaaat
     3781 gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa aactaatcaa
     3841 aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc gcagcttcag
     3901 cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat caaacatata
     3961 gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa atccgttcag
     4021 gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc agaaaaatgc
     4081 aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt gaagatatcc
     4141 attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca caagcagctt
     4201 tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa agtgaacaac
     4261 cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt acgatcaaag
     4321 aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat ttatgaggct
     4381 gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac cagttgtctt
     4441 gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc gagtaacgaa
     4501 agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac cgttcttatc
     4561 tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc cagaatacta
     4621 caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg gtgtattccc
     4681 ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga tcgaaacctt
     4741 agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc tgatgaactt
     4801 tattggtggt tggataactg attttactac tggatttgta tgcagacagc aaggaatcgt
     4861 tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta aatgtatggt
     4921 gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat taggccgaca
     4981 aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat gatcgtagat
     5041 aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc aatacctgat
     5101 agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg aatttatcct
     5161 aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc attagcacaa
     5221 tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca gccactaatg
     5281 gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa gtacaaatat
     5341 gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat agaaaaaatt
     5401 ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga agcagtagat
     5461 acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat tggtgtgata
     5521 caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc aggaaatgca
     5581 gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc tttgcttggt
     5641 ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat tgggaaagga
     5701 aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca aaatgcatgt
     5761 gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt agaagtaggt
     5821 gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat cattgcttgg
     5881 gtagcaagtt ttggattata tagctg
//
LOCUS       DADXTG010000133         5901 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.136, whole genome shotgun sequence.
ACCESSION   DADXTG010000133 DADXTG010000000
VERSION     DADXTG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.136"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            199..555
                     /locus_tag="IWI57_002338"
     CDS             199..555
                     /locus_tag="IWI57_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBC2450443.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            565..1464
                     /locus_tag="IWI57_002339"
     CDS             565..1464
                     /locus_tag="IWI57_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBC2450444.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1981..2712
                     /locus_tag="IWI57_002340"
     CDS             1981..2712
                     /locus_tag="IWI57_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBC2450445.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2857..4884
                     /locus_tag="IWI57_002341"
     CDS             2857..4884
                     /locus_tag="IWI57_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBC2450446.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            4992..>5901
                     /locus_tag="IWI57_002342"
     CDS             4992..>5901
                     /locus_tag="IWI57_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBC2450447.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYT"
BASE COUNT         2037 a          991 c         1223 g         1650 t
ORIGIN      
        1 acaaataaca ggttgctttt tgggtctttt tccgttcaag aaactgaacg aagaaaaggg
       61 aaagttgtct cttttgtgat aagagaaatc ataataaacg aatgacttac tttccaataa
      121 attgagaaca gtcagaacca gacaaattag ttgcatctga tgaataaagt gattattcta
      181 tgacatgagg tgatacagat gaaattagaa gtgacaaaag aagcacaaga agtattgaag
      241 aataaattag aagcagacga tcaactttta ttagatatcg aaaacggtga cggtccattt
      301 gcagaaagcc aagtcagttg ccaattggat acggcatttc gattgatcat tgtcaaaaaa
      361 gatacacaaa cagatctaag tctttattct gtagtggctg atacaccagt tggaccaatc
      421 aaaatcaaag acagtgcaat actttacatg gatgatccat ccacacttgc tttagaacca
      481 acttataata gtttacaatt aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt
      541 gttcgaaaag actagaggga taaaatggga aaattggcga tcatcagcga cctacatgtg
      601 gacatcaata aattcggcga atcagagctg atgcagcttt ggcaagtttt gcaaaagcac
      661 aagatcactc ggcttcatct agccggagat acggctaata aagtagaccg ttgcctagct
      721 gttgtcgaat ttttcgtcaa taaaggatta tctacgacat ttcatttcgg caatcacgag
      781 ttggcagatg tgcccaatga acagatgatg gaccattttc cagacccaca ttttttaaat
      841 gaacggtata ttgctttaaa tgagcagacc gttcttttag ggatgaatgg ctggtacgac
      901 tatcagtttt ccgaattgaa agattacgag gcaatcgtac gaatgaagcg tttgtattgg
      961 tatgatcggt tcatccagcg tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca
     1021 gctgcaaaaa atttgctaga ccagctggaa gcaaaaaaac ttgaggtgat tttatccact
     1081 catttcgttc ctaaacgggc atttatcatg tatcaatctg ctccttatga gcgttggaac
     1141 aaattaaatg catttttagg atcagaatca ttcggagaat tacttgaccg ttattcaaat
     1201 atcaaacaag tagtttttgg ccatacgcat cgtcgctttg aagataaatc catcggtcac
     1261 acgatttaca gctgcagacc ttttggttat tattatgaat ggcagctgac ccgagatttt
     1321 gtcttgaaag aacagttgct agacagctat gatcctatga aattacgtgt cctcttgcgt
     1381 gcccatcacc cagcttttca ggcctatcaa acacaacatt tgcaagagga atttgaacaa
     1441 gcgatgacaa tcgttccgta ttgagacagc attagtaatg tgtaaaagaa aataccttgt
     1501 aaaacataag gataagtaag agcagtagat ccatttatcg tggatatgct gctttttttg
     1561 cggaaaataa tcagcgaatg tttatttgat cggatgattt gtaactcgca gagtactaga
     1621 tgcaagcgtg tattcttttt ttgaacgata atcacaaaaa aaaatataat atacattttt
     1681 tatttaatta taattaatat tgattgaagt ctaggcagtc aagtcacatt ttgatagata
     1741 ggagggggac ttacgaatta ttttaaaggc tatgacagct gtatttctag attgtgtttg
     1801 catcaagttg caataaaaac tagaaatcaa atcgggctga taatcattta taactaatcg
     1861 atacatagga ccaaatatag gtctatgttc ttttatttca taagtatcgt aaaacagaat
     1921 tttgctatgg attaatccaa tcgactgata tgtttagtaa ataaagaaag aggaatgtca
     1981 atgatgaaaa aagaaatcgt attaacagca gcaatgatgt tattcagcat ggtagcaagt
     2041 acgacttttg tttctgcaac tgaagtatac ccaaaggaat ataacacaga aggaacgatt
     2101 acatttgaag caggagatga gggtgtaact cctcctgttg atccagagaa tcctgatccc
     2161 aataaacctg tcgatccatc agatcctcca agccctggaa caggaggggc attatcgatt
     2221 gattatggat caaaattcaa atttgggact caaaaaattt ctactgcgga taaaacgtat
     2281 tatgcagctg ctgatgtgat gaacgatggg tctagaaaac cgacatatgt ccaagtgaca
     2341 gataggagaa gtaccttatc tgggtggaaa ttaagtgtct ctcaaccaga acagtttaaa
     2401 actgccagtg gggatgaact agttggagca cagctgaaat ttacgaaagg gcaggctgtt
     2461 tctttggttg atccaacata tacgccgcaa acggtgaatt ctgaattaac gttgacaccg
     2521 ggaggaaaca atacgttagc aattaatgct aaatcaggaa caggtgtggg gacttgggtc
     2581 tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg tacagctttc ggtacccggt
     2641 aaatctgtca agttagcgca acaatattcc actaaattag tttggacatt agaagataca
     2701 cctaacaact agtttactga aaagaactgt taaaaataaa tacgtagtca tattgtcttg
     2761 cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat ttgtttggat acgaatagtt
     2821 catctaggaa tttaacgatc aggaggaaaa gatatattga aaaagaggag gatcgtgctc
     2881 attttaagca gtctcttagt gactaatatc tttccgatca tagggtactc tttggaattg
     2941 aatcagttgg agcatggaaa tagaagagac gaagaaagca aactcacttc gacattcgac
     3001 agtttaatag aatccaacca gacacaagaa agcagtgagg atttaagtat ttcgagtagt
     3061 ggagagacca ctgacaacgt agaagctgag ggttcggaca aacaaggact agaagatgca
     3121 ccaatttctg cggttgagct gcctgacgat cccaatatta tttctatcga taaaattttt
     3181 caaacaccta tcggtactag cacgtccata ttggaaaatg ggaagttact gcagttgaac
     3241 ccagcagaaa ggtctcaaag aggagctatt tggtcaaata agaagattag tttactctcg
     3301 gattttacat ttaaatctta tttatactta ggaaatcaac gagatagagc gggagatggc
     3361 atgaccttta cattgactgg cgatcctaga atggctagta atcccagtca agttattgga
     3421 tcgccaggta tgggaattgg ctcatattct actcgttctg gccaacctta cattgcgaat
     3481 gcgctctcga ttgagtttga tacgtataaa aatagtggtt cttctgatcg aatggatcga
     3541 gagataactg ctgataatag agggcatgga catgtcgctt ttgtcacgcc taaaccaaat
     3601 aataataatt attctggaga gcatactggc gtgacggtag ctcctacatt tctttctaat
     3661 ggcacatggc gcttgttaat aattaaatgg gacgcggagc aaagaagtct tacttatgat
     3721 ttggaaggtg tcggtcgctc ttctcggatt atacctgatc tcattaggca gtttggttct
     3781 acagaagtaa attgggggtt tacttcttca acaggtactt tttttcaaga aaatgctgtc
     3841 gctgtcacgc gacttccatc gaatgtcaca tcaacagctg atgtaaaagt caacgatagt
     3901 gattatgggg ccatcgctga agctggagga aacgataaga taactatcag aaatacattg
     3961 aaggtcgagg gggattttct tgaatataag aaaatgcaga ctagtatcga cctcccacaa
     4021 gaattaacta ttcccgagca tatcttgatc aatggaaatc aaattgatgc caaagatatc
     4081 ttgataaccg gaaatcgagt cacgctaaat ctgacagatt attttattgc tggagatacg
     4141 ttaataatcg aattggagac aactttgcag gaagtaattc ctgaaaaagt actcactatg
     4201 cattttgaat accttgaaaa cgggaatttg attgagcaat cgaatgaaat aaacctaaag
     4261 atcgcaaagc tgaaggagat agatatccat gtttattata aagataaaga cagtcagatt
     4321 gatattgctg aaatgaaaac aatcactggg aaaataggag agaagtataa agaaactcct
     4381 attgatattg aagggtatgt ttttgactcg gattcagcga atacagaagg catcttctcc
     4441 gaaaatacag aggatatcta tttttattac agaaaaggca aactttatct ctcaactgct
     4501 cctcaatatg tcaattttgg aaaacataaa atatccagta gaccattaag tgaattcgga
     4561 cagttttcag gtgggttgaa aattatagat gaacgggctt ttggaagttg gaaattacaa
     4621 ttgaaacaag agaaagttct tacaaatgga gattttgagc ttccagaagc tctttccttt
     4681 gttacctctg aaaatacaac ggttataggg acatccgctg ttacgatttt cgaaagtgat
     4741 caaaagggtg aaagcgattt gtcctctctt ttagattcta cagctcatcg tggaatcaga
     4801 ataaatattc ctgtagaaaa ccaaagaatc ggaacctttg aggggaaact atcttggata
     4861 cttgcagatg tcccaggaaa ttaacaggaa gagaagaata taacaaaatt attgagtttc
     4921 aagaagggca caggtaccat aggctcttgt gtcccacaga gacaagattt tgttatagtc
     4981 ttctcttaca tatgattaaa ggagcgattt tcttgagacc aaaacaacaa ttatttataa
     5041 cactcctagt tattttaggg gcacttttta acaatatatc agtgtcagct agtgcattta
     5101 atttttcagt gacgccgata acttcagaga atcaaatcga caagagaaaa acttacttcg
     5161 atcttcaact tgatccggat caggaagtag aagtaaaagc ggaattgaga aatgatacag
     5221 aaaaagaagt gaaaatcgat atttctgtga atagcgcaac caccaattca aatgtgatgg
     5281 ttgaatacgg aaaaaacgag attgaaaaag acatgagcct gatctttgat ctggtagatt
     5341 atgtgagcta tccacagata gtgacgctaa aacctaaaag tgtccaaacc gttgcttttc
     5401 atgtcaggat gccgaatgag cgatttgatg gtgtattggc tggtgggatc acattccaag
     5461 aaatccagac ggaaaaggac caaacagaaa ctaaggatca aagcttatcg attgagaatg
     5521 aatacaccta tattattgct ttgttgatgc aacagacact gaacgaagtt gctccgaacc
     5581 ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag aaatgtcatc ttagcaaacg
     5641 ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat tgaaacaaaa attacaaaaa
     5701 aaggacattc tgaagtttta tatcaagagg aaaaagaagg attgcagatt gcgccgaata
     5761 caaatttttc ttttccaact gctttaaatg gtcaaccgct aaccccaggt gaatatcacc
     5821 ttacaatgac tgttttagga aatgaaaatg aaaatggaaa attttcgaga aaaaaaggaa
     5881 atactacgat caactatacc a
//
LOCUS       DADXTG010000134         5895 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.137, whole genome shotgun sequence.
ACCESSION   DADXTG010000134 DADXTG010000000
VERSION     DADXTG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.137"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..238
                     /locus_tag="IWI57_002343"
     CDS             23..238
                     /locus_tag="IWI57_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450448.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(455..711)
                     /locus_tag="IWI57_002344"
                     /pseudo
     CDS             complement(455..711)
                     /locus_tag="IWI57_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(898..1809)
                     /locus_tag="IWI57_002345"
     CDS             complement(898..1809)
                     /locus_tag="IWI57_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2450449.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(1828..2832)
                     /locus_tag="IWI57_002346"
     CDS             complement(1828..2832)
                     /locus_tag="IWI57_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBC2450450.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(2829..4952)
                     /locus_tag="IWI57_002347"
     CDS             complement(2829..4952)
                     /locus_tag="IWI57_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBC2450451.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(4957..5877)
                     /locus_tag="IWI57_002348"
     CDS             complement(4957..5877)
                     /locus_tag="IWI57_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2450452.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         1708 a         1258 c          993 g         1936 t
ORIGIN      
        1 tgagaggaga atcttcattc ttatgaacgc tggaattgcg gatatgaatt taataaaaaa
       61 aacccttaat gattttactt ccaattcaat atcaaaaggt actggtatca atttaagcac
      121 aataaaaaag ctgaaaagtg gtgaaagatc agtggaaaag ctgaatcttt tagatgctat
      181 aaaaataacg gaatttgcta tgaaaaatgg aaaagctgag attgaaatat ggagataatt
      241 atcgtctagt gaattacttg ccacttagct gaatttaacg atccttggtg cttgaagggg
      301 gagattcttg ggaatagagc atacttgcaa gcctatgtct ttactaacaa cgatatctct
      361 tattaaccaa gctatccccg tagttctaag gcttttactt ttatctaagc aataatcgta
      421 ttcaaaggaa atttttgacc cttagaaagt ctcattttat cagacgtgag cttaggcatt
      481 gctgaatttt gttaattcgg ttttaggttg tgctttgagc ataatatgaa agtattcttt
      541 atcacgattt cattcatcta aggtaatgtg atgtgattct gaaattcttt caaaggcagt
      601 ttttgcataa gctgacatta catcatcaat gacatgttta caatgtttca ctactaaaat
      661 aagggggtaa ccaagaagaa aaactgaatg attatttgta tctaattcca tggtatatca
      721 accatttcct atatagactg attatgacat ggattaaaat aaaaaggcac tatgtcttaa
      781 ttgtcggttt tcgagttacc gaatggcatt catcacccac ctatagagga tgggcgactt
      841 ctgcctgaaa taagttacac tacttataac ttctaataaa attagattga gtcattatta
      901 tttaacatca acgattttcc agtttccatt tttttgtaat actaatttgt actgaaagta
      961 gctagtggtt tttgatacat tatcaaggta ttttatcttt gtactcacta gaaaattttt
     1021 tccgttttgt agataaactg gattctctaa ctccgaaaac tttaagtcaa tattgattgg
     1081 tcgcatcgca ccattttcaa cataatactc caattctttt tcagaagctg atggataaag
     1141 tttaaaaaat gtttctaaaa aatcggtgat ttcttccgtt gttttggcat caatttctga
     1201 atcgttttct acttgttttg cttcatattt tgccttgtct ggctttccaa tcattgttgg
     1261 caaactagtt actaaatgat tcctgttttc atcttcaaag attgtcactc gatatgcaga
     1321 agaaattgat tgcgtctttt ttccctctgt aatatgttgc tccactaaaa atgaaacgac
     1381 aaattcttcc gagtgttttt caacatctag aatttttaca gattgaactt cggaactcgt
     1441 gggaatatct gctcgaacca tatcttggat taaagcgaga ccgttttctg taaggtactg
     1501 ttcgagatta ctcattctct tttcaattgc ttctttgtta tttttccatg aaaaatattc
     1561 tttcgcaaaa tcctctgtaa aactttcaac gccagaagta ttcactacct tctcttttat
     1621 aaccactttt tcatgagttg tatgttgatc aatggcggtg aaatttttat aaacgccaaa
     1681 agcaagacta caagacaata aaagccaaag ggcaagcacc atttttcgat gcttacccac
     1741 ggatacaact cgttcttttt tcttttttgg gctcttttcc ttttgattcc gttcaatttt
     1801 tatcttcatt ctttttcctc ttttctctta tttgtatcgg ccagctccga taagatgctg
     1861 ccgccaatag ttttccgtta gattcgtcca ccccactgga tctcctgcat gaaacatgcg
     1921 attctcccct gcgtaaatgc ccacatgggt cacataagta ccggcatcat aggttgaatg
     1981 aaagaaaact aaatcacctg gtttggcatc ttttagatca atatgagtca tggcatcata
     2041 ttgatcctga gccacccgag gaagtttcag gtcaactttg gcataacacc attgggtcaa
     2101 gccactacaa tcaaaccccg tttctggact tgagccacca aacacatagg ccgttccttc
     2161 atacttaaat gcctcatcaa aaatttcttg gactattttg tcatcaaact gagttgtagt
     2221 taggtattgc ttcactaata aacagtaaaa catattgcca taattatacc gccacccacc
     2281 attttcctta atcgcgattg ggttggggta atcgactttt tgaccacctg atttttcctt
     2341 tgaaaaagat tcagctaaag caaaactatg cttcttcccg tttttcccga catgatctag
     2401 atacccgcca ccatagttat aggcctgaat aatactatct tgatcacacc ctagttcttt
     2461 ggaacgctta accaaagacg caaaatagag acagccttgt ttgatggatg cctcggtact
     2521 taatgaatta ggtggtaatc cggctgactc agaactttgc ataacgtctt ccattgtgcc
     2581 accagattcc acttgaataa tcgccaaaag aattggaata tactctgtta tatccgattc
     2641 tttggcatac ttttccacca ttgcacggtg tttcaatact tcttctgaga cactagttcc
     2701 gccaggagaa gtgccagaac taccatttcc ctcactgtct tcaccaaaca acaaacccac
     2761 acacatcaaa atacccataa aagagatggt cagaaaagtt aatgaaataa cacccacttt
     2821 cttcaagttc attttttctc acctgacctt gattttggac gatggttcat tcttttttgt
     2881 actttttgat tggtctgctt tggcagagtg gacctctgtt tcacttgttg gaaagaattt
     2941 ttcttacttg ctgattggct tttccctttt gaaatgattt cttgactagt tagttttcgt
     3001 ttgatttcgg gattcagatt tgctctcaca atccgctttg gtgcttcttt tttcagcatc
     3061 atatctttct gaggttgagc tgctttatct attggaatat tcgcccttct ttttccctga
     3121 cgatttgttg cttcactcag tatcttgcgt ttttcgtcca ttttcgcatt tcttcgttgt
     3181 cgttgttttt ctcgcaaatc cactcgatcc gctttttctt ggaacagtcc gcgcttgaat
     3241 tcttctggtg cttttttcga cttttcgatt cctttatgga gattgtattt caaattcgtt
     3301 ggcgtattcc ttacctgatc ctttacttgt ttagtcttat ctttcaccaa agcttgggta
     3361 tccaaaactt tgccgatttt tctcccagcc aaattcattc gggattgttt cttattattt
     3421 gaaactggat tctccttagt taagtcagat agtatttcgt gatcgttcgg taatcgtgaa
     3481 ttctttcgta gagaagaacc ggatggctta aatttggatt ttgattgttc ctttgccaac
     3541 ttatttccga ctgctgcacc accaccaaaa gccaaagctc gtcccacatt tcgattgagt
     3601 tgccgcaatt ttcgattcat taacatttgc ggttttcgca tgacgcgtct tcccattgac
     3661 tggctatcat tactctggag gttgaacata ctcataatgt cgcccaattt gaaatagata
     3721 ccggcaaagg tcacaatttg cagaaaagca accatgaaaa atggataggt tgccgaaata
     3781 ttgaaaaaca tcgtggagat gctaaatgca gtcgtaatca ccaaagtaac acccgctcgc
     3841 atcataatcg tattgaataa acgaataatc gccttctttc ctaaactttc ataagtcggc
     3901 aacatggaca ataagaaact aattggcaga aacatcgcaa agataatgaa gagaatttgc
     3961 gagaacaaca taatccctgt tagaagaaaa acaaagaagg aaatgccaat attgaacaat
     4021 accaaaaaga ccacggtccc taaacggttc atggtcttgg taatactgag attcgcattg
     4081 tcattgtctt caatctctgc cttgaccgct tcttcccgat ctttgccttt gttttcatct
     4141 ggactcacag atagaatctt gttgacccgg tcttcaccaa tttcttctat gttggaatca
     4201 tcaaattgca gtagtaacca aggttgttgg acttgaatcg aaaacaaact atcccgaatc
     4261 aaatccacac tatcttttcc ttggctttct gaattgggta caacgatctc ggttccaagg
     4321 gtaagggccg cttcactaac atctgagcta aagtcattga tttttgtgat ataggtaggc
     4381 gcataagcaa tgaacgaacc cgatagtaga aaaatcatca caaaattaat cacggctcga
     4441 actgctttcg tggtttctcg ttttaataat ccggtatacg ccacatacac ccccagagct
     4501 aaaatcatca ataatagaaa cccaaaataa aatccggagg tctggagtcc attttcagta
     4561 ataccagcca atgtttggat attctttcca atactttcag ccgtatccga aatgaaatct
     4621 aatttgtaag cctcttgaac cacataaccc gtggcgttcg acaaatacaa actcaccgtc
     4681 caaatgaaat tagtaatggc ataaagacca tacatcacgc ttttccctaa gccatcaccc
     4741 caattccaag gcaaccaatc ccaagagcta tccacaaaga aatctagttg atagttgttc
     4801 agtgaatatt tggaatatag atttcctgct tcaactgtat catcaactag ccctgtcgcc
     4861 tcggctaccg taccaactaa actcaataga actatcagag tgagtatgct aataaaaacg
     4921 attcccacaa tccgaaaaag tttctttttc atcccctcac tccattctct cttgaacagg
     4981 tggacgggta tcaaaggcat ggaagagttc ttcaaagact ggatgtacct gaaccacgcc
     5041 tacccgacca tacaaatcct gcatcaagca ttggccattt tccaaatctc gtaggcgttt
     5101 ttgattgctt tcatcttctc gatccaagcc aaagaattcc aaggttttct tgatttcatg
     5161 aatatcggta ctgcgaaacg caaacttcaa cccgatatta ttcttcaagc gttcatccag
     5221 taaatcatca ctattttgcg tgacaaagta aaccccggca ttcatagccc gcccagcacg
     5281 aacgagctta ttcgataatg ttttcccttg cgctacttga agaaaactcc acgcctcatc
     5341 taagtctaca attttgaaga tttctcgatt actatggata aagtccaagg caaaagtgga
     5401 aatgacgatt agcatggaaa cactaagcaa ttccatggtg gtatattctt caaaagtggt
     5461 ttctgcatct ggtaaaacca aatccgctac ttgaatgata tttaactgcc tatctaaact
     5521 aatcgactgg gtaatcgaac catctgagaa aagcaaatgg gcaaaatcat aatcggtaaa
     5581 ggactcgatg tgatccgcga tattttccgc aactggtgag ccttcttttc gaagctcatt
     5641 aattacttgt aataatcccc gttgattgct ttgggtaacc gtccgaatgg ccttacgcaa
     5701 cactggaaac ttctccccat ctcgactaga aattcctgtt aaaaaggtca gaatatcaat
     5761 tgctagactt tcagaatcct tctttttctc cataatcaca taaggatcaa gtaatccttg
     5821 attttttggc ttactagtta agttgagaat attgatttca tccgcaattt ctggcaaggt
     5881 ttctttccag ccacc
//
LOCUS       DADXTG010000135         5874 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.138, whole genome shotgun sequence.
ACCESSION   DADXTG010000135 DADXTG010000000
VERSION     DADXTG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.138"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            641..1375
                     /locus_tag="IWI57_002349"
     CDS             641..1375
                     /locus_tag="IWI57_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2450453.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IWI57_002350"
     CDS             1391..2560
                     /locus_tag="IWI57_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2450454.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IWI57_002351"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IWI57_002351"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBC2450455.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IWI57_002352"
     CDS             3650..4576
                     /locus_tag="IWI57_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBC2450456.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALSQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IWI57_002353"
     CDS             4595..5845
                     /locus_tag="IWI57_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2450457.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1202 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgagtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DADXTG010000136         5826 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.139, whole genome shotgun sequence.
ACCESSION   DADXTG010000136 DADXTG010000000
VERSION     DADXTG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.139"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            352..1041
                     /locus_tag="IWI57_002354"
     CDS             352..1041
                     /locus_tag="IWI57_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2450458.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IWI57_002355"
     CDS             1028..2236
                     /locus_tag="IWI57_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2450459.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IWI57_002356"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IWI57_002356"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBC2450460.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..>5826)
                     /gene="guaB"
                     /locus_tag="IWI57_002357"
     CDS             complement(4350..>5826)
                     /gene="guaB"
                     /locus_tag="IWI57_002357"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBC2450461.1"
                     /translation="WETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKLNIP
                     IISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPFFLT
                     PQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMTKDN
                     LVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADATLI
                     AGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVARQYG
                     KAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRGMGS
                     LGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGYVGA
                     ANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1781 a         1108 c         1141 g         1796 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccag
//
LOCUS       DADXTG010000137         5784 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.140, whole genome shotgun sequence.
ACCESSION   DADXTG010000137 DADXTG010000000
VERSION     DADXTG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.140"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..834)
                     /locus_tag="IWI57_002358"
     CDS             complement(<1..834)
                     /locus_tag="IWI57_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBC2450462.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QR"
     gene            complement(1039..2847)
                     /gene="pepF"
                     /locus_tag="IWI57_002359"
     CDS             complement(1039..2847)
                     /gene="pepF"
                     /locus_tag="IWI57_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBC2450463.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(3023..4021)
                     /locus_tag="IWI57_002360"
     CDS             complement(3023..4021)
                     /locus_tag="IWI57_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBC2450464.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(4247..4900)
                     /locus_tag="IWI57_002361"
     CDS             complement(4247..4900)
                     /locus_tag="IWI57_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBC2450465.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(5232..5630)
                     /gene="spxA"
                     /locus_tag="IWI57_002362"
     CDS             complement(5232..5630)
                     /gene="spxA"
                     /locus_tag="IWI57_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBC2450466.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1725 a         1190 c          937 g         1932 t
ORIGIN      
        1 tcgttgagag tgggaatttc ctttaagtcg taccgtggtt tctttcactc gatgattcaa
       61 gaaagttctg ccaatatgtt ggacgatgtg gaaacgatcg atgatcaact gagcattcgg
      121 gaaaagcttt ttcaccaacg aaacataagg cgcatacata tcaataacaa tcaattgaac
      181 ggcttcacga gtcgctaatg aaaaccgaga aaaatagcgt tctaaataag gcaagcgtcg
      241 attttcgaca atatccaata atgcatggct ttgtccgtcc atcatgataa aactcattgc
      301 actggcaaca cttcgaaccg atttgaattc atcaaagcat aagacctttg gcaaagtctg
      361 tttgaaagga ttcaatgggc ggtagaatcg cttcatgaca cgatagaccg aagaagtgga
      421 aatatttttc tgacgagcga tttccgtcat ggaagtattc tcttttaagc gagcaatgac
      481 agaccaacga actttttgac taatgaaaca gtgtttttca gcaacagagg tatccgctac
      541 gaatgttctt tggcaagact tacatttaaa tcgttgtttc ttcagctcca gataagtgcg
      601 atactcagac acatcattca gtaacaaccg aacggttgtc gtcccccaac gaatgatcct
      661 agaggggacg acacaaagac aatgggaaca ctcttctggc ttgtacagaa gagttccttt
      721 gtaaatcaag caaatttgcc ctttgtattt ttctttagtt aaacaatttt cctcgaaata
      781 gatatttagg tctagaatat ttagggtatc tttgataagc tgagtatgag acatcttgaa
      841 tcctcctatg aattggtagc caaattaatt ctaaaggatt tcagggtgtc ttttctatat
      901 ttttttatac aaaaaagatg ttggtgaagt tcaccaacat caaaaagtat agagccttaa
      961 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
     1021 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
     1081 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
     1141 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
     1201 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
     1261 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
     1321 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
     1381 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
     1441 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
     1501 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
     1561 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
     1621 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
     1681 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
     1741 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
     1801 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
     1861 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
     1921 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
     1981 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
     2041 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
     2101 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
     2161 aaattgttcg taaacactgt acaacccctt aaatgctgct tcgcgcaccc ttcgatccgt
     2221 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
     2281 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
     2341 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
     2401 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
     2461 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
     2521 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
     2581 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
     2641 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
     2701 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
     2761 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
     2821 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
     2881 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
     2941 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
     3001 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
     3061 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
     3121 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
     3181 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
     3241 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
     3301 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
     3361 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
     3421 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
     3481 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
     3541 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
     3601 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
     3661 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
     3721 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
     3781 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
     3841 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
     3901 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
     3961 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
     4021 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
     4081 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
     4141 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
     4201 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
     4261 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
     4321 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
     4381 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
     4441 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
     4501 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
     4561 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
     4621 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
     4681 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
     4741 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
     4801 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
     4861 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
     4921 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
     4981 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
     5041 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
     5101 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
     5161 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt tccaagtttc
     5221 gtattatcct tctataatcc agccatcaat tgtgcttgac gtaattctaa ttgtcgtaca
     5281 tcgcgcggta ggaaccgacg aatttcatct tcattaaaac caacttgcag acgtttatca
     5341 tcaatcatga ttggacggcg aagcaatcca ggattgtttt gcacaagttc caataattct
     5401 tgtaacggca gatcatctaa atccatgttc agtttttgga atactttgga acgtgtcgag
     5461 atgatttctt ctgttccgtc ttctgtcatt tgtaaaatcg cttttaattc agaactgttc
     5521 aatggttctg aaaaaatatt acgttcaaca aatggaatct gatgttcttg caaccacgca
     5581 cgagcctttc tgcaggaagt acaacttggg gaagtatata atgttaacaa cgtgcaccac
     5641 tccttttcgt gataagaatc acttttttgt aacagaagat cataataatc tcccatctgt
     5701 ttctattata cataatcaac ttttaaatag ctaccttttt ttcataattt ttttaaaaaa
     5761 atgaaagtta tctaaggtta atat
//
LOCUS       DADXTG010000138         5660 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.141, whole genome shotgun sequence.
ACCESSION   DADXTG010000138 DADXTG010000000
VERSION     DADXTG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.141"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..275)
                     /locus_tag="IWI57_002363"
     CDS             complement(<1..275)
                     /locus_tag="IWI57_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450467.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYT"
     gene            complement(495..861)
                     /gene="ssrA"
                     /locus_tag="IWI57_002364"
     tmRNA           complement(495..861)
                     /gene="ssrA"
                     /locus_tag="IWI57_002364"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1032..1805)
                     /locus_tag="IWI57_002365"
     CDS             complement(1032..1805)
                     /locus_tag="IWI57_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBC2450468.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1949..2299
                     /locus_tag="IWI57_002366"
     CDS             1949..2299
                     /locus_tag="IWI57_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBC2450469.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2280..2996
                     /locus_tag="IWI57_002367"
     CDS             2280..2996
                     /locus_tag="IWI57_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2450470.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2996..4444
                     /locus_tag="IWI57_002368"
     CDS             2996..4444
                     /locus_tag="IWI57_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2450471.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4514..5023)
                     /locus_tag="IWI57_002369"
     CDS             complement(4514..5023)
                     /locus_tag="IWI57_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450472.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5013..>5660)
                     /locus_tag="IWI57_002370"
     CDS             complement(5013..>5660)
                     /locus_tag="IWI57_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450473.1"
                     /translation="FSAFLLIACLTLYHLQSALANQVQDRLSFIGASASTELLPALEL
                     ASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWHLTGLSKTKLFLFIFWQLFF
                     PLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRDILTSVSGLTIPMDQQT
                     FFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1921 a          995 c         1109 g         1635 t
ORIGIN      
        1 aaagtatact tcaaatagtc ataacgccga tgatcgctcc gtgcgtctgg cacatcagtc
       61 acgatatcaa acaaaaaata cacatctttc ttcattctgg tttttgttgc cggaatttta
      121 aagtagttct tattagagta atagagattg attaataagc tatcttcgat tgctaaaaag
      181 aaaacttctg aatcccacac cttataaaaa tctttgacaa atctattcga aggatcaaat
      241 ttaaaccata attgtgtttt tccttccatc aacataatgt tcacctcaaa aagagtatac
      301 gaactaatgt tctttttgta aagtaggaat atcattgata atatggatgg aacatatata
      361 ttaaaaagtg ttctttttga atgctatttt gaatgccatt ttcattcatt taacgaaaat
      421 ctgcgaaaat aaaaaatgct caaaccctta taaaataagg atttaagcat ctagcgaaat
      481 ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc gcgtccaaac acatcgccac
      541 ttaaaaatct acgttcatag tcaactcatt taaggattca ctttacagct tgccgagtga
      601 caggcattct gtatcgctag tctgatgttc tctttttcaa cttacagacg gaaagttgaa
      661 acgtatccca ctaaagttag gacccttact cgagcacatg ggcgatgccg agaggatcta
      721 cgctaactgt ttttaggcag ctaaagcgta agagttgttt tcgtttttag cagttatatt
      781 taactgtaac gtttttacgt agacgtaacc tacgaaacgc aattcaagct cgactgtgcc
      841 tgtcgaatcc gtaacgtccc cgttagctaa caagggttac gtgtattttt catacaaaat
      901 tagtatagca tatattggga taaattcaat aaaaaagcga atcatatccc attatttata
      961 taataactgc ccctttctta agaaaaaata agcgattttc ttaagaaaag aggtagcttc
     1021 aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc gccttctgta
     1081 tttttctttg tgataaaggt agaaagttct tttaatccgt cataggcgtt gcccatagcg
     1141 attttgttgt cgcaagcttc caacaaggca aaatcattcg gaccatcgcc aaatccaaaa
     1201 gtaggaactt tttccagctg aagttctttt tgcagacgtt tgacacctgc cccttttgag
     1261 attcctttct tcactgtatc gatcgagtaa ggaccattcc gataaaaagt tagctcagga
     1321 aaatgttcgt gatagtattc gtcacctgat tgagataaga tcagcaacat atttactgtg
     1381 ttttctttaa agcctaaagg atcaatttcc ggaacatctg aatgaatgaa gtcgtaagca
     1441 ttgatcattg tttggctatg accggtacac cagattttct gatcgttata gtaagaaagt
     1501 tcatgaccgt tcaactgtac atgatcatac attttttgac attcttctga agaaaatgtt
     1561 tgagaataga tttcttcgcc gtctactcga atgaaagaac cattcatggt gaccgcagaa
     1621 gtaatgcccg catcttccat gatagagtgg atttctgctt ctgtacgtcc tgtagcgatg
     1681 acggggagaa cattgttttc ttttaatgcg ttcatagctt ttgccacttc gggtgtgatt
     1741 tttgatttcg catctagtaa tgttccatca agatcaaaga aagtgattgc acgataattt
     1801 gacacaaata aaactccttt tttattggaa taagtttatc ttaacaaaga atgaacaaaa
     1861 gacaaacctt tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt tagagtatac
     1921 tataagaaga gaatttagga gggaaagaat gatttttttc agtattggta tcttattctt
     1981 aataattggc agtctttttc ggattctgcc ctcaaaagga aatctgcctt tttatgggta
     2041 tcattcgcct cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa agacaagcgg
     2101 aaactggttt tttcttatgg gcttactgat ggcattgatc ggctattatt taaagacatc
     2161 tgggcatacg aattattttc tgattgaaat gcttctgcta gtatttccaa ttatgcctat
     2221 ttttattatg acagaaaaga aattacagaa atatgacctg gaaactggag ggaacgataa
     2281 tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag atgtttttct
     2341 taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc gatgcctttt
     2401 tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc acactggacg
     2461 gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt atcatgctga
     2521 cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca gatgattatg
     2581 tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg catcgccgta
     2641 gtgagttggc agaagcaaga gaagaagcag tagtttcaga tgatcatttt gatatcgaga
     2701 ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac cggccaattg
     2761 aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag
     2821 tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt ggtgatgaac
     2881 ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag
     2941 tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt gcctaatgcg
     3001 ttatttgtat cagcaactct tagctttctg cggaatggtc gcgttgatct tattgatcgt
     3061 tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt accagcaact
     3121 gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata cgctcccgca
     3181 gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag tactgacaga
     3241 acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc cgactaatac
     3301 agctttactg aattttacct taacagctga tcaatggaaa aatctgagga atggacgtca
     3361 agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct cttacgcact
     3421 tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc aacctgcgcg
     3481 aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat ttattttttc
     3541 cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta agcgtatcaa
     3601 tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac agctccccgt
     3661 acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga cgaattcatt
     3721 gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga
     3781 tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg aagggttaga
     3841 atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga aaaatgaaac
     3901 agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca
     3961 aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca
     4021 gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg ttgatgatat
     4081 tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc
     4141 gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct atcttgaaac
     4201 gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga aaagtatctg
     4261 ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg aatcaggatt
     4321 aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag aagtagaaag
     4381 cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaga
     4441 gtaaaaaaca agcggtataa aagatcatga atcgatcttc tataccgctt gtttttacta
     4501 taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt tgagcaatag
     4561 tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa taagaaagca
     4621 agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct tcaataacaa
     4681 tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt tcaaaagacg
     4741 tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt tcttgatagg
     4801 ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg tctttaagat
     4861 tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc agttttgcaa
     4921 caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga ggacacaaaa
     4981 taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc aggcctccta
     5041 ctttgcaaat gatggataaa aaaagtgaaa gggaatagac taagaatgag acagcatcca
     5101 ctgagcccaa taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt
     5161 tggaaatcga tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa
     5221 gtcaagatat cacggatatc tggcagttca gtagtcttta agacaagaaa attgatcctt
     5281 tgaagaagcg cttcataaac tgactggaag cttacgatcc acaataaaac caagatacaa
     5341 caaagaaaga gaggaaagaa cagctgccag aaaataaaaa ggaataattt ggttttagaa
     5401 agaccagtca ggtgccaatt gatcaattcc tgcttgtttt gcctgactga atagtaaaag
     5461 aaaaagataa aaaggaaggc aaatagaaaa aataaactga atatgccttt tatataaaat
     5521 cggctagcta attctaaagc cggaagaagc tctgtagatg cagaagcgcc aataaatgaa
     5581 agccgatctt ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt caggcatgct
     5641 atcaataaaa aagcagaaaa
//
LOCUS       DADXTG010000139         5562 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.142, whole genome shotgun sequence.
ACCESSION   DADXTG010000139 DADXTG010000000
VERSION     DADXTG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.142"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..648
                     /locus_tag="IWI57_002371"
     CDS             7..648
                     /locus_tag="IWI57_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450474.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            707..1147
                     /locus_tag="IWI57_002372"
     CDS             707..1147
                     /locus_tag="IWI57_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450475.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1159..1827
                     /locus_tag="IWI57_002373"
     CDS             1159..1827
                     /locus_tag="IWI57_002373"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBC2450476.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1891..2514
                     /locus_tag="IWI57_002374"
     CDS             1891..2514
                     /locus_tag="IWI57_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBC2450477.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2570..2956
                     /locus_tag="IWI57_002375"
     CDS             2570..2956
                     /locus_tag="IWI57_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBC2450478.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3169..4560
                     /locus_tag="IWI57_002376"
     CDS             3169..4560
                     /locus_tag="IWI57_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBC2450479.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5171..5539
                     /locus_tag="IWI57_002377"
     CDS             5171..5539
                     /locus_tag="IWI57_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBC2450480.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT         1840 a          923 c         1176 g         1623 t
ORIGIN      
        1 gatactctga ttgagcgatc tacatttcta tatgttcaat tgcaggaaaa taaaacattg
       61 ctgcattttt caccagagtt ccatctagag gggctaacat taggagaaat ctatcaagca
      121 gaagggctgt cggctgtttt gcactttttt aaggtagaat tggaattacc agtaaaagat
      181 tatatcgagc tttctcttga atcttgggat cgagcagtgg ttgaactgtt tccagaaggt
      241 ttaatgatcg atacaaacga tgggaagatc catctgactg cagcagaact gcaaaaacaa
      301 atgcgttatc aaatagatga cgaaaattct ttttctattt ttagacgtca gcaaaagatt
      361 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
      421 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      481 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      541 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      601 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      661 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      721 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      781 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      841 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      901 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      961 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
     1021 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
     1081 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
     1141 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
     1201 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
     1261 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
     1321 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1381 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1441 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1501 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1561 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1621 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1681 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1741 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1801 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1861 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1921 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1981 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     2041 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     2101 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     2161 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     2221 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     2281 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     2341 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2401 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2461 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2521 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2581 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2641 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2701 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2761 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2821 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2881 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2941 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     3001 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     3061 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     3121 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     3181 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     3241 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     3301 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3361 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3421 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3481 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3541 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3601 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3661 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3721 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3781 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3841 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3901 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3961 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     4021 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     4081 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     4141 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     4201 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     4261 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     4321 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4381 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4441 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4501 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4561 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4621 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4681 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4741 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4801 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4861 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4921 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4981 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     5041 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     5101 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     5161 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     5221 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     5281 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     5341 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5401 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5461 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5521 tgcggttaca ggtaaataat agaaataaag actttgtttt tc
//
LOCUS       DADXTG010000140         5442 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.143, whole genome shotgun sequence.
ACCESSION   DADXTG010000140 DADXTG010000000
VERSION     DADXTG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.143"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(190..588)
                     /locus_tag="IWI57_002378"
     CDS             complement(190..588)
                     /locus_tag="IWI57_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBC2450481.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(585..1358)
                     /locus_tag="IWI57_002379"
     CDS             complement(585..1358)
                     /locus_tag="IWI57_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBC2450482.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1355..2038)
                     /locus_tag="IWI57_002380"
     CDS             complement(1355..2038)
                     /locus_tag="IWI57_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBC2450483.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2205..3092)
                     /locus_tag="IWI57_002381"
     CDS             complement(2205..3092)
                     /locus_tag="IWI57_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2450484.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3089..3958)
                     /locus_tag="IWI57_002382"
     CDS             complement(3089..3958)
                     /locus_tag="IWI57_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2450485.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4095..5381)
                     /locus_tag="IWI57_002383"
     CDS             complement(4095..5381)
                     /locus_tag="IWI57_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBC2450486.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1669 a         1218 c          901 g         1654 t
ORIGIN      
        1 ttcatttttc tccccctttt ttcatcattt ttaaaatagc atattttgaa aagaaagtct
       61 gatgcttaga gtagaatttc ataaaaattt actatttcat aagtattcac gagaaaaata
      121 caaacaaaaa agcggaaata acgctcatcc gccttcttgt ttgtgtttat ttcctcttca
      181 aacgcttgtc tatgattcag acttttctct atcgttattt tcatctaaaa gttcttgcat
      241 tccgataatt tctcgatatt gatgaatctt agtgatacca atgactttgt ctacatcaat
      301 actaaaacaa ataccattac ctggaatatc gatgttgatg ccttgaccaa tcgcatccat
      361 tactttattc gtcaaactat cgggtactaa tgtcaacaca atatcttttt ctggcgaaat
      421 gtccaaacct aaaaatttcc ctgaatcatg tacaccagaa cctcttccat gtaagatcgt
      481 tccgcctgct gcacctgctt cttttgaaaa gtcgataaca tcttcgccca ttcctccgtc
      541 aacgatcgtc acaatcatgt gaagattcaa tgctacaaga tctttcattt gctttccccc
      601 aatttttgca atctttcttt ttcgattctg tctttttcat cttttcgttt ataaatatat
      661 cctagtacta aagtggataa tattggtgtc aatgcaatca ttgcaaccac accaaacccg
      721 tcaatgatcg gatctcttcc ttcaatttgt gcagcgattg ccacgctcat tgataaaata
      781 aatgtcgaac acatcgggcc cgttgctact ccaccgttat caaaagccat tgctaaaaag
      841 atcttgtcca cttttcgtcc taaaataaat gctagcccgt atcctgggat caagaaataa
      901 taaagagaga atccgacgag caaacgccac atagacaatc ctactgctgc gccgatccca
      961 attgaaatta ccatcagcat gattttcgat cggattctcc cctcgctcaa ttcttcaacc
     1021 tgtttgatca ttacatgaat cgccggctca gcaaacccta ctaagaaccc cattaaaaag
     1081 ccaaggggaa ccagtatata attgtaaggc aatcccgcaa tagcactgcc taaatgctgt
     1141 cctaccggta cataagccat attgactccg tgcaggaaaa ggatcaaccc aatagtcgtt
     1201 atcaagaaac ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa actaatgata
     1261 ttcattaaaa caaataggat tacgataggt gccactgcca aaatgacttc catggtaacg
     1321 atttcgattc catctatcac ttgctgccag aaactcattg gaagatcacc cccaaaatca
     1381 tcacggcaag aatcggccct aacgaagcaa taccgaccat tccaaaactg tcattttcat
     1441 ttttcttttg tttgatcata ctagtcatcc ctccggcaag ggccaagata aatggaacag
     1501 ttactggacc agtagtcact ccccccgaat caaaagcaat cggcataaat tcggaactgg
     1561 tgaaaaatga ggcgatcaaa acgcctatgt agcctatagc catcaattga taataggata
     1621 gcttgaatac gacccgaagc agcgcaaagg ctagaaatac tccagtgccg acgctgacga
     1681 tcccaatcag caacactctg ccaatctctc cgtcagatat atcatatact tgttgtccca
     1741 aaacttgcac ggaaggttca gcaatcgtga tcacgatccc gatagcaaat cccaaactga
     1801 ccaaaacggt caaacttttc tttttgatca tatatttccc tactagatct ccgacttcca
     1861 tcatagaata atctgctcca aacaaaaaga gcgtcattcc gatcatcatg attgccgcac
     1921 caaccagaaa tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac gttaaaatga
     1981 caatcaggac agtcatcggc aatatagcag caatcacttc tttaaaattt tttagcatgt
     2041 atcctcctcc tttactcttt aagtttacct taaaatgaaa gagttttcga gcacaaactt
     2101 aaaacgttat catttaattt ttctcaacat aaaataacac acgtcccaaa agtatctttt
     2161 tacttggaaa aatgactttg aaggaacgcg cttattgtgt ttttttattt tacagagccg
     2221 atcattcctt gaacgaattg cttttgcaaa acaaaaaata cgatggcagt tggtaaagta
     2281 gcgatgacta atccagccat gatcattcca tagtctggtg tataggaagc acccatagca
     2341 gataaaacaa gcggaaccgt ccgtttatca ggagattgca aagctactaa gggccataaa
     2401 taattgttcc agctactcat gaatgtgatg attgcagccg cggcataggt atttttagcc
     2461 gtcggcatat acactctaaa aaaaatactt agttctttta gaccgtctaa tcgagcggct
     2521 tctactagat cttttggaaa agatttaaca ttttgtcgaa agaagaagat caaaaaagca
     2581 gtgctaacag atggcaggat gacaaccaag aaactgttga ttcccaaaac cgttccgttc
     2641 aattgtgaaa acattcgata taaagggatc atcaatgccg caaaaggaac catcatcgat
     2701 agcagtagaa tattgaaaat taggtctttt cgtttacttt gataaatctc aaatccatat
     2761 cctgccattg acgaaatcag caatgcaaac acagtggtca ccaaagcgat catcgctgaa
     2821 ttccaaaggg aacgagtaaa attcagctca ttggaaaaca aattttgaaa attgatgatc
     2881 aagttactgc caatctttat ttttcccgat gtgatatcca cgggtgtatt cgtcattccc
     2941 aaaaccatcc agacaaaagg gaaaatggaa ataaatgata ggatgcttaa tgctgtatac
     3001 tttaatactg acccaattgt cgaacgaagt tctaatgctt gttttttgac gttggggcgt
     3061 tcgattgttt ctttttctac catatttttc atcgactttc tcctcctact ttcgtttgga
     3121 ttatcgacaa tatcacaata aaggaaacaa taacaaagga aactgctgcc gcatatccaa
     3181 aattaggcgt gaatttataa ctcaaattat agatgtattg agatagggtg gtcgttgcat
     3241 ttgctggtcc cccgccagta atattttgta cttcatcaaa aagctgcaat gtcccgatcg
     3301 tcgaagtaat ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc actttgataa
     3361 actgctgcca tttagaagca ccgtcaatag atgctgcttc ataaatctcc gaatcaatat
     3421 tttgcatgcc tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa gtgatcgaaa
     3481 gaatgatcag aactctcgcc caaattggat gagtcaacca gccaatcggt gtgtcaatca
     3541 taccaatgcc caacaacaag ttgtttacaa ggccagattg agaaaataga cttttcatga
     3601 tcagagaata actgaccatc gaagtgatac atggaagaaa gatagcggtt cgaaacaacc
     3661 ctttatattt caacttttta tcatttaaaa ggttggaaat aaaaagtgct agaaacaaca
     3721 tgatcggtac ttgtacaagt aaatacagga atgtattaaa aaaagctttt ttaaaagttg
     3781 catctgtcaa catccgctga taattaccta acccgttaaa tgtcatattg acagggggac
     3841 cactttgaaa agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg attgatatca
     3901 aaatgactgg caagattaaa aaagcatgcc cgtttttgaa taaatctttc tgcttcattc
     3961 ttttccctcc aattctatca ttgattatct acatgaaaag acaagaaaga caatatctgt
     4021 aaggcagacg tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga agaaattcac
     4081 ttttttacca ccgatcaatt agccaattgt gcttctactt gtttttgtgt atctgctagt
     4141 accttgtctg tctcttctcc attgatgatt cgatgaagag cttcagctac gactgcttca
     4201 attgcatagg tctcatgtcc gtaattcact tctggtattt cagatgtcca tgttgaaaaa
     4261 tcgtcaaaga tcttttgacc actgtaaaat tcagaagctt cttgataagc ttcttgttca
     4321 ttcgcgctaa gcatcgtaga tactagtccg atttctttcg caagagtgcc catcagttct
     4381 tcattcgaag caaacgttgc ttctaggaaa tctttcgctg aatcttctcc agctactcct
     4441 ttgatcacat accagccagc gccgccaagg ttagaagctt gcgctttttg cagattttct
     4501 ggaagtgcag gtattggtgc aatcttccat tttccagatt ggttttctgc tccttggatc
     4561 gttgaagaat accaagcgcc agttgggcta gaagctactg ctcctgattg tatcgcagtt
     4621 actccagcat tccagtcaga ggtttgttcg actagatctt ctttcaaaag agtggcaaac
     4681 aattcaagac catactttaa agatgcattg tcttggatcg tgactgtctc cccatcttca
     4741 gatgtatacc attctcctgc ctgttgcata atcatcctta ctcgaccaag gtcagaagga
     4801 ttgacttcag cgattttctt tcccgtcttt tctttgacat ctcgcgctac ttgaatatac
     4861 tcgtcccact tcagattctc catatcctct tctgtataac ctgcttctgc catcagatcc
     4921 gtacgataga agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat tttatctcct
     4981 actttgttta ccgcaaattt atacggtgca aaatgatctt cctttacaat agcagacaaa
     5041 ttagcgaatg catctggata agaagtcaaa tagccttgta tcctataatc ttcaatcaag
     5101 acgatattgg gcaatccttc tgtattcccg ctcgctaaag cggtgttcag tttctgaacg
     5161 atatcatcct gagacatcgt aacaacttct atctctgttt cttcgttctc ataaactttt
     5221 tttgcttcat tcactgcttt gatattgaat gtctcatccc acgcccagac agtgactttt
     5281 tcttctttgt ctgctgtact actagcactt ccgcatcctg ttaagaaacc tgatgctgcc
     5341 gctaaacaaa cgactccagc aattaacttt ctgatgttca tttttatccg ctccttttcc
     5401 atccattagt tgtttacgtt tgctacattc atcataccaa aa
//
LOCUS       DADXTG010000141         5383 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.144, whole genome shotgun sequence.
ACCESSION   DADXTG010000141 DADXTG010000000
VERSION     DADXTG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.144"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            234..1685
                     /locus_tag="IWI57_002384"
     CDS             234..1685
                     /locus_tag="IWI57_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBC2450487.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2034..2666
                     /locus_tag="IWI57_002385"
     CDS             2034..2666
                     /locus_tag="IWI57_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBC2450488.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3254..4693
                     /locus_tag="IWI57_002386"
     CDS             3254..4693
                     /locus_tag="IWI57_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2450489.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4988..5206
                     /locus_tag="IWI57_002387"
                     /pseudo
     CDS             4988..5206
                     /locus_tag="IWI57_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1793 a          860 c         1100 g         1630 t
ORIGIN      
        1 ctatagaaga acaaattaca ttagacaaat tgaaaatgtt agattattta tttcatccta
       61 gttttagcca accctataat tttattttaa aggcagtaac aggattaaac attgaaacat
      121 aatcgttact aaatgaaatc aagaaatata ctgtattact caattcattg aaaatattta
      181 tcataatttt taatagaaaa cactattaaa gagaaaaaag agaggtaaat caaatgaaaa
      241 aatctttaaa acttgcatca gcagttatat taatgtccca actccttata gcaacacctt
      301 tccatgtgtt ggcaagcgaa aattcaacat acaaaattgc aaaagaaaga accgagacga
      361 ctgaaatgag taattccgat gtaacagcag agagtagtga acttttgaca acttcatcaa
      421 gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcatcaagt gaaagtagta
      481 cgacttcaaa tgccgagtct tcaacaactt catcaagtga aagtagtacg acttcaaatg
      541 ccgagtcttc aacaacttcg tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa
      601 caacttcaag tagtacaagt gaaataactt cttcgaaagt gacaaaacca aaccatatag
      661 aatcttcaaa aaaggaagcg ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac
      721 agcaaataga atcaacagaa tctttccaat cacaaccaat actatctggt aatgaagaaa
      781 ttcattttga caaaaatgaa tctgtagaaa attttattat gaaaattggt gagtctgcga
      841 gaaaaattgg tcagaaatat gatttatatg cttctgttat gattgcacaa gcgatcttag
      901 aatctgccag tggtcaaagc cagttagctc aggcaccgaa ttacaattta tttgggataa
      961 aaggaactta taatggaaac tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa
     1021 cgctttatac gacccaatca aaatttagag tgtatgaaaa ctatgaggaa agctttgaag
     1081 attatgccaa attattaact aagggaatta gtggaaataa agatttttat gcaggagcat
     1141 taaaagctaa tagtaaaaca tatcgagaag caactaaatt ccttacagga cggtatgcaa
     1201 cagatacaca atactatttg aagctaaatg aattaattaa aacctacgat ttaaccaact
     1261 atgataaaga agttaaaggt ccacaaatga gtaagactgg ataccaagtg cctattcata
     1321 attattcaat aaccagtttg tttggaaatc gtggaaatga atttcaccga ggacttgata
     1381 tggcagctac acagggtgag ccgattcatg cagttaaagc tggtaaggtt gttaaagcag
     1441 agtttcacta ctcttgggga aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt
     1501 tatatgctca ccagcaacaa tacctagtaa aaactggaga taatgttttg caaggacaaa
     1561 taattggcta tgtaggaagt actgggaata gtactggccc acaccttcat ttggaaattt
     1621 gcaaagatag tagtctttct cagagcatgt tagttgatcc gaaaacctta ttattcggag
     1681 actaaaaaag gtaatcaatc tgacaaagta acaagaaaat ggttggattc ttagaccttt
     1741 gatcaggata ttgagactgg aattgatatc tcgattacga tgggtttgac aaataaaaca
     1801 agtccattat cgaatatcga gtgtattatt gccgtcttta tgctcatatt ttgagcaggt
     1861 ttcactagat gaaaacaatt tatctatctt gaggatttcg tgtacgtatc gttcagtctt
     1921 gtattgtaaa ttagtcataa aatcagacta agaaaaattg gaaatatttt tttctgattt
     1981 attaaaagtt aattgaaatt atatggtact tctttcttgg gagggtaggt gcagtgaagg
     2041 aaagatatgg tcaccttact gttgttgagg agattactca taatggatat aaaaaatatc
     2101 gttgcctatg tgactgtggg aatgaaacga ttgtatatcg atcaaactta ctatcaggta
     2161 gaaccaaaag ttgtggatgt ggaaattata aaaataggca taaatataaa aatttgacaa
     2221 ataaaacttt tggtaagtta gttgcaatca agccaactaa actgcgaagt gaaggaactg
     2281 ttgtttggga atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc
     2341 taaaaggtga tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata
     2401 aaaaattcgg attacttact gcaaagtatc ctttggacaa taactgttca tggaaaacaa
     2461 aatgggtttg tcattgtgag tgtggcagta tgtgtgagat aagttatagt aacttatttt
     2521 ttggacatac tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag
     2581 ggacagtaat agagtgtcta acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg
     2641 gagtttatcg ttctaaagag aaatagattg cgtatataac tttaaaaaaa aagaggtact
     2701 atttaggatc gtatgattct attaagagtg cagctcaagc tagaaaagat gctgaaaata
     2761 aattgtttca acctttgatt gacaaatatc gaggtaatca ttaattattg tggataataa
     2821 ttaacagtga tatataaaca tctataatat attattcata ctattgatat ttagtataat
     2881 cctatggaat tgaatatcat agcaatgtat ttgaaaagca aattggaaaa caaataggtg
     2941 caacaccata aatatgaaaa aattgggaaa aagtagaatg gaaatttgct tacgtctcac
     3001 gagatagctc acactattaa tctgaagaac aatgaagtcg acttttagaa attgctagaa
     3061 gaaagtaaag cgaattatca ctagttacta tagagtaagt tcaaataaag catctccaac
     3121 ttacatggtt acatgaggat atcaaatcga aattactcaa gcttcctatg gctagagtcg
     3181 cgagaagttg ttgggcagta gacataggtt atattgttct tagctacacg atcattagaa
     3241 aggaaatcct attatgtata gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg
     3301 gattttgatt gctatatcta tgtttacctt catgtccaca ttggatggca gtattgtcaa
     3361 tattgcacta cctaccatct cagatgatat gggcatacca atgaatcaag cggaatgggt
     3421 agtgtccgtt tatctgatga ttgtctgttc ttgtttgttg ctgtttggta aaattggcga
     3481 tagccgtggg aaaatcaaag tatttcggat tggtacgctg atctttgtct tgggatcgct
     3541 gttgtgtggg ttcaatcatt cgctaggtat tttattgttc gcacgggtgg tacaagccat
     3601 tggtgctagt atgactatgg ctacaaatgc cggaatcatc acggaagtct ttcccataca
     3661 tgaacgtggt cgggcattgg gagcaatagg tgcgtttgtt tcattaggct cgattgctgg
     3721 accaggtctt ggcggcttga ttttgtccca attttcttgg gcgtacattt tttggatcaa
     3781 tgtaccggtg ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac
     3841 catgagcggc catacaatcg atggaggggg ctttgctgcc attgcggcta cgattatcag
     3901 cttttttgga gcaatctttt tggggcaaga aagtggcttt ttccatccag tcccccttgt
     3961 actgttcgta atttcagtga ttgcctttgt cagcttttta aaagttgaaa agaaagcagc
     4021 ctctccattg atttctttcg gtatttttaa gaacaagatt tttaccatga gcttgattac
     4081 agccttgttg attttttctt ccaacttctt tgtcaatgta gtgattcctt tttatctgca
     4141 aaatgctcgc gggttgagtc ctagctatgc tgggctgttg atgatggtct ttcctttgat
     4201 gatggtcgtc ggggcgcctt taagcggcta cctgaccgac cgggttggcc cggagatttt
     4261 ggtattagta ggcttgggct tattgtcggt aacccaggtc atgtatatgt ttttgtcagt
     4321 ggactctcct ttgtggctat acattgtcgc tactgccatc atgggtgccg gcaatgcctt
     4381 gttccagtcc cccaataata cgatggttat gagctcggtc actatcgaaa acttgggact
     4441 ggcaggctcg atgaattcct ttgcccgaaa tttggggatg gtcagcggga tttcgctttc
     4501 tacgacggtc ttatatcaag cgatgtctgc taagatgggc cagcaagtaa ctaactatat
     4561 cgcaaatcgg ccggatgtct ttttatatgg aatgaagatt acctttttag gcagctttgt
     4621 tttgtgtttt gttgcctttt tcctgactat ctggcgtatc aaaaaacaag gaggtattca
     4681 gaaaaatgtc tgaaaaaacc actgttgaag aatttttgga ggcctgcgta tgggtgagtg
     4741 ggatttccat ggatgatgga agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt
     4801 atcttcaaaa tactagtgct caatgagatg cttgatttga tcacaagaag actgcgaaag
     4861 ccgctggcaa ccgcaagcta ggtatcccac cgatggacta cgttttagag gctactggtt
     4921 atgaatacgg agccaacgct ctcatctgca taagaataca tcacccagat tatgtaaaga
     4981 ttttcgatac ttctgtcaaa aaatcattaa gatagataaa tgatttccgt ctagtcaaat
     5041 tggttcagaa tgtagacaca cagatggtaa gaagtcactc acaattcgag aatggacttg
     5101 tcctatttgc cgtatacatc ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa
     5161 gggctatgaa tacaagcatt gccttagaaa acatcaagaa ccttaggaac aacggggata
     5221 gcttggttaa taagagatac cgctgtttag gcttgcaagt atactctttt atcaagaatt
     5281 ccgtggcttt agtcatgaga ggcttaatac tatgaaaatt ttctttagca tttttgattt
     5341 aggttccttt tctaattata ataagatgac cactacagta acc
//
LOCUS       DADXTG010000142         5352 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.145, whole genome shotgun sequence.
ACCESSION   DADXTG010000142 DADXTG010000000
VERSION     DADXTG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.145"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..433)
                     /locus_tag="IWI57_002388"
     CDS             complement(<1..433)
                     /locus_tag="IWI57_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBC2450490.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEG"
     gene            complement(549..1184)
                     /locus_tag="IWI57_002389"
     CDS             complement(549..1184)
                     /locus_tag="IWI57_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBC2450491.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(1267..1689)
                     /locus_tag="IWI57_002390"
     CDS             complement(1267..1689)
                     /locus_tag="IWI57_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBC2450492.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(1707..2027)
                     /locus_tag="IWI57_002391"
     CDS             complement(1707..2027)
                     /locus_tag="IWI57_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450493.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2327..3064
                     /locus_tag="IWI57_002392"
     CDS             2327..3064
                     /locus_tag="IWI57_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBC2450494.1"
                     /translation="MNTPQIFNFEQNEVRTFLENDIPYFVANDVAKTLGYKNPSDATN
                     KHCKKAVKTWGSDSLGRRQSFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLE"
     gene            3088..3291
                     /locus_tag="IWI57_002393"
     CDS             3088..3291
                     /locus_tag="IWI57_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450495.1"
                     /translation="MTDIAEITQRDREKIKAYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            3307..3627
                     /locus_tag="IWI57_002394"
     CDS             3307..3627
                     /locus_tag="IWI57_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBC2450496.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            3632..3811
                     /locus_tag="IWI57_002395"
     CDS             3632..3811
                     /locus_tag="IWI57_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450497.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKASREGLTR"
     gene            complement(3931..4509)
                     /locus_tag="IWI57_002396"
     CDS             complement(3931..4509)
                     /locus_tag="IWI57_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450498.1"
                     /translation="MVVFSMNYILYVLLVVYAIIYIKQIVNAVSEITTINYLLTTIEK
                     VINDLKRLNDHSGSYKSDPYFQNNVTPIREIMVKEMPRINKVLPYSLVTLSLNQSDDI
                     LEENFRIIASQIFDKNNEIHYRKFWFLNPLIALKKFFLLPSEILSWFGINLSTFPGRI
                     FSLVIWVTSLFAQETISQILSFVITHFFKGQK"
     gene            4548..5246
                     /locus_tag="IWI57_002397"
     CDS             4548..5246
                     /locus_tag="IWI57_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBC2450499.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNMKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
BASE COUNT         1834 a          886 c          910 g         1722 t
ORIGIN      
        1 atccttcttc tacggcatct ttcaacgatg ctctaatatg gttattaaac ttaataacgg
       61 tggattttac atggtctttc gcatacttat ttaaaaagcg ttgatatcca actctatcta
      121 aatcagaaat caataccgct ggcatatatt tctttatgtt cattaacgta tcttcgtatt
      181 ttctataagt gataggagat actgttcctt ctttataaag ttgcatccaa tcctcaaagt
      241 aatctgataa taatagattt ttgcgatccc ctttaaggcc tttgttcaag ttatattcta
      301 attcgttggc ggcatctttt gcttcgcctt ttgtcttaaa tcctgatttt cttatcttcg
      361 tgtattttcc gtcatctttt ttgtatgaaa tttcgtattg ccaactattt ccacgtttga
      421 ctaatcttgc cataattgaa tcagctctct ttctctgata caataggaac tataaagaag
      481 cctactgtat aggtttgttt tttcatagaa cacgcttgct ttggacggtg ggcgtgtttt
      541 tatatcaatt agttatgaag agcaattgaa actgtctctg ttccaaagta acctgtttgt
      601 gcttgcaata cattattttg tgcatttgct tgggcttcag agatatcaaa aactatttta
      661 ccagtttgtt gcatatcagg atttaagctt tccataaaga aagagttagt gatattacca
      721 ttttcatctt gatttgccga catagatgca gccgaatcag cttcgaaagt ttttccatct
      781 gcttttaatt tgaagaatga gctatcaaca gtcacagcct tatcacctgc attttttaca
      841 gataagtcaa ctactaagaa agtgtctttt gcgttagtag gaagtacaga tgggccaact
      901 tgttttacta cttcaacgga gtttactttg tattccattt tccctacagc aacgtcatca
      961 ccgattttat aagtcttttc ttcttttgct gtagtttctt ttgtagtttc tttattagta
     1021 cttgttgatg ttgcagtagc tgaatctttg ttttctccgc cacttagagc accaccaata
     1081 ccaaaaaaca aaagtaatat taatacccaa aaccacactt ttttgtaaaa aggtttacta
     1141 actttataca ttttcccatc ttgacccata actttttttg ccatttaaat attcctcatt
     1201 tcttgttata atatatttgt gatctcagaa atgaggtatg agtccgtgtt gcagcacggg
     1261 ctttttttac tgtgcataag agtatttttt cttgaaataa gactggcaaa cattaaaaca
     1321 ttctgttctt aacttattat tgatagaata gaactccatg aaattttcta atttgaactg
     1381 agattcatca gtcagttcgt tctcaataaa gatatttagt aaaatcataa ttgcgattct
     1441 atcagcttca gtttcgaatt ttgaatgaaa agttgtagag ttatcgtaca gtactgaata
     1501 ttcaaaatgt gaagcaatga aatgaccgag ctcgtgggct aaatgaaaag cttcagaact
     1561 gtcttcgtgt agtttttcat tcagaaatac tattcttggt tttggataat aaaaacctgg
     1621 ttcttccatt tccatataga ttaactttaa attatactca ctcagcattt ctttcaactt
     1681 taaatacata caacccatca ctccaactat tcattttcct ctaaagcttt agcaattgca
     1741 atcgctttac gcattgtctc cttagatatt tcttttccat caaaagaaaa aacagtatcg
     1801 tcttctgata aatccacatg tttaggggtt tcttttttat ctcttcctag aagatagtct
     1861 atagaaacac caaaataatc tgatactttt gccaaacctt cggagttagg tgatactgat
     1921 ttccatttac tgaaatatcc atttgaatat cccaatcttt tttctaattc ccttacggat
     1981 attccgtttt gctttgttaa ttcttttatt ttttcgtatg gattcattga tatgtcaacc
     2041 tttctagact gacaagaaaa aatatagaat aaaactctat ttatctgttg acaaattaga
     2101 gttaaacctc tatacttgtt cttgtaaaca aatttaacaa ctaaaaagac aacaaaaaac
     2161 acttttgatt attaaacgcc aaccgccaaa aaagctttta aaatcaatgt ttatatgtct
     2221 tatttaacta tgtacaaagt atagaataaa actctatgct ttgtcaattg aatttagaaa
     2281 aaagttgtta aatttgttta cgaatataaa agaaaggaat gaaaaaatga acacaccgca
     2341 aatttttaat ttcgaacaaa acgaagttcg gacgtttcta gaaaatgaca ttccgtattt
     2401 cgtagcaaat gatgtcgcta aaacattagg atacaaaaac ccgagcgatg ctactaataa
     2461 acattgtaaa aaagccgtaa aaacatgggg tagcgattcg ctaggtcgtc gccaatcttt
     2521 caaagttatt ccagaatcag atgtttaccg cttgattatc aaatcgaact taccaagcgc
     2581 tgaaaaattt gaggcttggg taatggaaga agtccttcca acaatcagaa aaacaggtag
     2641 ctattcaaac gtacctcaaa gttttgcaca agcattgcgt ttagcagcag atttagaaga
     2701 aaagaaccaa ttactcgaac aacaaattgc cgagtacgaa ccaaagatta gctacttaga
     2761 tacgattctt tcatcgacag atacggtagc gacttctcaa attgcagctg attacggaat
     2821 gtcggcaatt gctctaaaca aattgcttaa cgagttaggt gttcaacata aagttagcgg
     2881 acaatggata ctttaccgaa aacatatgaa ccaaggatac acaaaatcgc acacaagtga
     2941 gattccgaaa gccgatggcg gcactaaagt tgtaatgaat accaaatgga cacagaaagg
     3001 gcgagtgttt atttacaact tattaatcgc agagggctat taccctcaaa tggatttaga
     3061 ataaattggt tagaaaggag ttttagtatg actgatattg cagaaatcac tcaacgagat
     3121 agagaaaaaa tcaaagcata tgtcgaaagt tcgaagttct taacttacac catgcttgct
     3181 gaaagatttg gaattagtaa aagctactta tctttaattt taaacggtaa aaagacttct
     3241 gcagaagcaa acagaattat agattcgatt atcactatgt acgaattgta aagggaggaa
     3301 cagcaaatgc aatatctaga agcgaaaatc ccaattccag aaggctatgt aattatctcc
     3361 caagtggatt atgaggagtt gaaaaaagct gatgatactg gtagatggat gacgttgcca
     3421 gaagtactag aacggattaa cagaaaatat gattggttta cttctagagt tttaaagaac
     3481 ccaagatata gaaatattat cgatatcgaa aaaaacaaaa atggatttgt ctattatcca
     3541 gttgaaggaa gagacacata tctattttta agaagtaaaa cacttgaatt tttagaaaca
     3601 aatttttcgg aaatcttaag gaggtaagcg gatgggaaaa tttaatagag cattagtgtt
     3661 cagcgcaccc ctaatcatct acgctttagg actttgggga agcagacaag cattaatcgg
     3721 cacgatcgtt tacatggttt ggatttttat ggggcttgat gaagctgagt acagagcaaa
     3781 aaaagccagt cgggagggac tgactagatg agaaacaaaa aaatatctct aacgctatta
     3841 actatcatca cgattgtcgt atcgcaacta ttctttttaa ctatattaaa cgacatatcg
     3901 atgtcggacc gattgttggt tcaatcagct ttatttctga cctttgaaaa aatgggtaat
     3961 tacgaaagat agaatttgag aaattgtttc ttgggcaaat aacgatgtta cccaaattac
     4021 aagactaaaa attcttcctg gaaaagttga tagattaatg ccgaaccaac taagtatttc
     4081 gctgggtaag agaaaaaatt tcttaagagc aatcaacgga tttaaaaacc agaattttcg
     4141 ataatgaatt tcgttgtttt tgtcaaaaat ttgagaagcg ataattctaa aattttcttc
     4201 aagaatatca tctgattgat tcaaggaaag cgttacaagg ctataaggca gaactttgtt
     4261 aattctgggc atttccttaa ccattatttc tctaataggc gtcacattat tttgaaagta
     4321 aggatcagat ttatagctac cactatggtc atttaatcgt ttgaggtcat ttatcacttt
     4381 ctcaattgta gtaagtaaat agttaatcgt tgttatttca gacactgcat taacaatttg
     4441 cttaatataa attattgcat aaacaactag aagcacatac aaaatgtagt tcattgaaaa
     4501 caccaccatt tttttaccta aattatacca aaaggagaga agaaaaaatg caagaattag
     4561 taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa agctttgaga
     4621 aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg
     4681 gacagatgtt tgtagaaggt aatgaaccag attcatacgg cagaaatcga cgagtttttt
     4741 tcattagtag agatggtttt ttcttgttgg ctatgggttt tacaggaaag aaagctattt
     4801 acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata
     4861 ctgttcctca aacaattgaa gacatgatga tctaccaact agaagaaatg aaagatgtta
     4921 aaaaagatgt ttccatgctt aaaaatacta tgcgaattag cggacaacaa gagtttgaaa
     4981 ttaagcaaaa aggaaatatg aaagttatgg aagttctagg aggcaaagaa agccgagctt
     5041 atgaagaaat cagtaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt
     5101 caatcccaag atatggcgag ttacctcgta aaagattcga tgatgctgtt tcatttattg
     5161 aaatgtggtt accagaaact gcgatccgca tggaaatcga tcaactgaac agacaacaaa
     5221 gacttttcgg tgatgaaaat gaatagagct gaagcgctaa gaatagggac ggtaattgct
     5281 aatcgctggt ggagacacaa taaaccaagc atcctaagcc aacaacatat tgataagcaa
     5341 aaagcttggc aa
//
LOCUS       DADXTG010000143         5274 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.146, whole genome shotgun sequence.
ACCESSION   DADXTG010000143 DADXTG010000000
VERSION     DADXTG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.146"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            211..1203
                     /locus_tag="IWI57_002398"
     CDS             211..1203
                     /locus_tag="IWI57_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2450500.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1219..2103
                     /locus_tag="IWI57_002399"
     CDS             1219..2103
                     /locus_tag="IWI57_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2450501.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2152..3759
                     /locus_tag="IWI57_002400"
     CDS             2152..3759
                     /locus_tag="IWI57_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBC2450502.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3760..5244
                     /locus_tag="IWI57_002401"
     CDS             3760..5244
                     /locus_tag="IWI57_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBC2450503.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
BASE COUNT         1672 a          847 c         1158 g         1597 t
ORIGIN      
        1 gaggccgcag aaaagggtgc ttaaaaataa tctgttgttt aaaatattat tatcagaata
       61 cttagataca aaattctggc acaaagtaaa aaaaatattg acttaaaaaa atggatatgg
      121 taatctataa acgaaaatag tggtaacgtt tccaaaatgc atcttcgtta aatttttatt
      181 tttttaaacg aaaatggaaa cgtttctaaa atgtattggg aggagattga attggaaaag
      241 acattgggac caggtggtga gataagggcg gtacaacggg ataaaaaaaa tatttcgaag
      301 tgggaaaaat taaagaaaca gttgcggcgt gataagtgga tgtacttgtt gctcttacca
      361 ggtatcatca tgacgtttat ctttaagtat gtgccaatgt acggtgctgt gattgcattt
      421 aaagactata atcctatgct aggaattttg gaaagtcctt gggtaggact agatcacttt
      481 agggaattta tcagttcacc aaattttacg cttttgctga gcaacacatt aaaaattagt
      541 atttatggtt tattgttagg tttttttcca ccaattattt tagcactgag cttgaatcaa
      601 attatcaata aaaaaataaa acagaaaatc cagttgattc tatatgcgcc gaattttatc
      661 tcagtagtta ttattgtggg gatgctgttt ttgttcctat ccgcaaatgg acctattaat
      721 gggttagtag aaaaggttac ggggcaacca attatgttca tgtctaatcc agcttatttc
      781 agaacgattt atattttatc gggaatttgg caaggaatcg gctggtcatc cattctttac
      841 accgcaacgc tggcgggagt cagtaccgat ttatatgagg cagcaaatat cgatggggca
      901 aatattttac aaaagatttg gcatatcgat ttgcctgcat tgaggcctgt gatggtgatc
      961 agcttcatct tggccgccgg cggtattatg aatgtaggat atgaaaaagc ctacttgatg
     1021 caaacttcaa tgaacatccc tgcctcagaa atcatttcca cttatgtata caaagtcggc
     1081 ttgcaatcag gtgattatgc ctactctgct gctgttggtc tgttcaatat gattattaat
     1141 gtcgttttat tactgattgt caacaatata accaagcggc taaacgatgg tcaaggcttg
     1201 taatgaaagg agaaaattat gaatagtgct ttatcaaaaa tcgaccgtca aatcttatgg
     1261 ttgaatcgag tgattgtaac ttttttagtc gtcatcacca ttgttccgct gatttatgta
     1321 ctggtagctt cttttatgga cccagctact ttgatcaata aaggtataag cttcaatccg
     1381 aaagattgga caatacaggg atatcaacgg gtatttgcag atgattccat tttacgcggt
     1441 tttgtaaatt cgttgtttta ctcttttagc ttcagcttat tgactgtatt tattacgatg
     1501 atcactgcgt atcctttgtc tcgacctaac ttggcgggga aaaagattat tatgactttt
     1561 tttgtcatta caatgttcgt gggtggtggc ttgataccta cctatttatt ggtaaagaac
     1621 ttgggcatgt tagatagtgt atgggcgatt atcgtaccag gtgctataaa tgtgtggaat
     1681 attatcttag cccgtaccta ttttagtgct ttgccaaaag aactttctga agctgctact
     1741 atcgatggcg ccaatgactt gcaaatactg ttcaaagtca tgcttccatt agcgaaacca
     1801 attatgttcg tattgttttt atatgccttt gttgggcaat ggaactcata ctttgatgcc
     1861 atgatttaca tcaaagatcc cgacttacag ccgttgcaac tcgtattaag aaatattttg
     1921 atccaaaacc aaccttcaca agacatggtg ggagccaata ctgccatggc agaaatgaaa
     1981 caaattgctg agttgatcaa atacgcaacg atcgtcattt ctagtctgcc attaatcata
     2041 atgtatcctt tcttccaaaa atattttgat aaaggtgttt tagttggttc tgttaaaggg
     2101 taagtattgt tcactatttg tatgaataaa aattataaag gagattttaa gatgaaagca
     2161 aaatggatgc tgccaatcgt ttttacttcc gtactaattt tgggggcttg tggaggctca
     2221 aaagaaaaga gtaaatcaag ccctgattat gaactagaaa atgtctcttt tccattgaaa
     2281 gaaaaagtat ctttaaaaat gatgtctcaa agtgcccctt tggctcccaa tgatcctaat
     2341 gacaaattga ttttccaacg attagaagaa gagaccaatc tccatattga ttggacaaac
     2401 tacagtagtg attttgccga aaaaagaaat ttggatatcg ctagtggtga tttaccagat
     2461 gccatcttca acgccgctgc aggtgactat gatttattaa actgggcgga aagtggcgtt
     2521 attgtaccga ttgaagattt gatcgaagat tatatgccaa atctgaagaa aatttttgat
     2581 gaaaaccccg aataccgaca aatgtctact gcacctgatg gtcacattta ctctatgcct
     2641 tggattgaag aattaggcga aggaaaagag tcgattcata cagtaaatgg gatggcttgg
     2701 atcaacaagg agtggttgga taatttgggt cttgaaatgc cgcaaaccac tgatcagttg
     2761 atgactgtgt tggaggcttt caagacgcaa gatccaaatg gtaatggtga agcagatgaa
     2821 attcctttta gctttattaa caatggagga aacgaagact taaaattttt aatgggggca
     2881 ttcggtctag gtgataatga tgaccactta gtcgttgata acgatggtca ggtacaattt
     2941 actgctaaca aagaagggta taaagaagcc atcaaatatt tcaatgaaat gaataataaa
     3001 ggtcttattg ataaagaggc tttcgaacaa gattggaata cgtatgttgc caaagggaaa
     3061 gaacataagt atggcgtgta ctttacttgg gataaagcaa atatcacggg tgcagatgat
     3121 agttatgacg tcttgccagt cttagcggga ccagatggaa cgaaacatgt tactcgtacg
     3181 aatggcatgg gtttctcaag agatcgtttc gtaattacta gtgcgaataa aaacttggaa
     3241 cttacagcta ggtggataga taaaatgtat gaaccgttac agtctgtcca aaataactgg
     3301 ggaacctacg gtgatgaagc acaggcgaat atttttgaat taaaggatgg tatgttaaaa
     3361 catttgcctt tagaggggac agcaccgggg gaactaagac aaaaaaccga agctggagga
     3421 ccattagcag ttcttgattc ttattacggc acagtcacta ctatgccaga tgatgcgaag
     3481 tggcgtttag atttgatgca tgacacgtat ttgccatata ttacagaaga caatatctat
     3541 ccacgtgtct ttttagaaaa agaagaaaca gatcgattag caaaaatcga aacggatatg
     3601 tgggattaca tctatcgcaa aagagcagag tggatcaccg gcggtaaaat cgatgaagaa
     3661 tgggaagaat accttgctga attggaacgt gtaggtttgg aagaatggat tcaaataaaa
     3721 caaactggct atgataattt tttgaaggga gcacaataag tgttagtaga agaaacggcg
     3781 attttggata aagcaaatcg atttattgaa atgatgcgag gaaaagttaa ccctcagtat
     3841 cgacaaaact atcatttttc cgcacccatc gggtggataa atgaccccaa tgggtttata
     3901 tattacaaag ggcattacca tctcttctat caatactatc cttacgatag tcgatggggg
     3961 cccatgcatt gggggcatgc gcgcagtagt gatttggtcc attgggaaga tctaccagta
     4021 gcgttagccc cagatcaacc ttatgacagc ggcgggtgtt tctcaggaag tgcaattgaa
     4081 agagacggaa aactatattt attttataca ggctgtcaag aaattgatgg tattagccat
     4141 cagcatcaat gtttggcggt ctctgaagat ggtattcatt ttagcaaata cagtggtaat
     4201 cctgtggtag cagaaaaaca gctaggtgaa aatgggctaa tacaagaatt tcgtgatcct
     4261 aaagtaattg ttcacaacga tggttactat atggtagtag ctactaaatc tacacagtct
     4321 actggtagaa tagtgatgtt tcaatcgtca gatttgtata cttggcactt ttactctgtg
     4381 ctgctagaag ggacttcaga gcaaggaata atgtgggaat gtcctgattt attccattta
     4441 gacggacgag atgtcttaat catgtctccg atacaaatca agaagaacgg actggaatat
     4501 cataatatca gttctaccat ggcttgtatt ggtgaggtaa actgggaaac aggtcagatg
     4561 atagttgata atttccatga aatggatttt ggaatggatt tttatgcacc acaaacaaca
     4621 agagatccta atggtggacg ggtgatgatt gcttggatgc agatgtggga tcgtacattg
     4681 cctacacatg agttaggcca tctttgggca ggctctatga gtttgccccg agagttaaga
     4741 gtgaaaaaaa atcgattggt acaaaaaccc atctcaactg tatatagtca tttagtttat
     4801 caacaaggca gtgaaaatat tgcagtgact gacacatcag tcgtatttcg aaacgcgctg
     4861 tctgattctt catatttaca tctagtcgca gatttggaga aagctgaagt ttttacaatt
     4921 gaattactgc ggtctaagac ggattgtctc aaactagcct atgacactgc tacacaggtt
     4981 tttgaattgg atcgtcagaa actaggaaga gagcttacag gggcggaaga agaccctctg
     5041 aatgtacgaa aagttgaagt acctttgatt gataatcagc tcgttcttga gatttttaga
     5101 gatacctcat caattgaagt ctttattaat gggttagaag ttatgacggc tactttctat
     5161 gagctggaaa aagctcgaga tatcttgttt cattcgatcg gagaaagtcg tattcaagct
     5221 tttgaaacag cgagtgtgaa ataaatgttt gattgcttag gcgatgaatc aatt
//
LOCUS       DADXTG010000144         5083 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.147, whole genome shotgun sequence.
ACCESSION   DADXTG010000144 DADXTG010000000
VERSION     DADXTG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.147"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..2376
                     /locus_tag="IWI57_002402"
     CDS             4..2376
                     /locus_tag="IWI57_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450504.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="IWI57_002403"
     CDS             2437..3897
                     /locus_tag="IWI57_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBC2450505.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..>5083
                     /locus_tag="IWI57_002404"
     CDS             3908..>5083
                     /locus_tag="IWI57_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBC2450506.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     "
BASE COUNT         1933 a          857 c         1093 g         1200 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaattttc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatctacca acaagagatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggtagac agcaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgttttc
     4021 aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagcccg ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 gaaatatcta agctacccac gaacagatac cccttatatt acagaagggg agtatgcgta
     4921 tctgttggat cacttagacg aatacaaaca ctttttaaag gccgaagcca ttcctactcc
     4981 tattcataca ccgaatagtc gatatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aagacggtca tgacggcagc ggcttttgaa caa
//
LOCUS       DADXTG010000145         5078 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.148, whole genome shotgun sequence.
ACCESSION   DADXTG010000145 DADXTG010000000
VERSION     DADXTG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.148"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..991)
                     /gene="glsA"
                     /locus_tag="IWI57_002405"
     CDS             complement(65..991)
                     /gene="glsA"
                     /locus_tag="IWI57_002405"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBC2450507.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1014..2225)
                     /locus_tag="IWI57_002406"
                     /pseudo
     CDS             complement(1014..2225)
                     /locus_tag="IWI57_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2260..3873)
                     /locus_tag="IWI57_002407"
     CDS             complement(2260..3873)
                     /locus_tag="IWI57_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2450508.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(3985..4194)
                     /locus_tag="IWI57_002408"
     CDS             complement(3985..4194)
                     /locus_tag="IWI57_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBC2450509.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
BASE COUNT         1428 a         1116 c          856 g         1678 t
ORIGIN      
        1 ttcagcctca ttgtaggtag ctttgagccg tttggacttt taccccagcc tctttcaaga
       61 atggttattc aaagatgtca gcgtctagtt ctttgactac atctttcaac aattggatac
      121 cagctgcgct gttaccaaat ggatctagtg ccgggctgaa gacacctatg ccgtaacgat
      181 ttggcacagc tgccatcaac ccgccgccta caccgctttt tgcaggtaca ccaacatgga
      241 cggaaaactc tcctgattca tcatacaaac cagcagttgt cataattgat ttgacgattg
      301 tagcactttc ttcagtaatg atttgtttgc cattccaagg agcaattcct ttatttgcca
      361 gcacagcagc taattttgct atgcctgtag cggtgacatt gagggagcat tgtctgaaat
      421 aagcatcgag tatctccggt acgtctcctt cgatcatttg attacctttc atatagtaag
      481 caagagagcg attgatatca cctgttcttg attcagaatg atagatttct tcattcaatg
      541 tgatattagg atcatcacag atctctttca taaatgacaa gattcgctca aatttttcat
      601 ctgcatcttt accggcaatc aacgaggttg ttgcaatcgc tcctgcatta acaaaaggat
      661 tcattggatg tttgcgatga ttgatttcca tattcatgat agaattgaag gcaaaaccgg
      721 ttggttccgt gttgattgtt tgaaagactt tttctattcc gttgtctttg attgctaata
      781 acaatacggg aacttttgac atgctttcga tggcaaagcg cacatcggcg tctcctccct
      841 caatcagttg ttttgtatcc aaatcataaa tcgccatacc taattgtttg ggatccactt
      901 cagctaatgc tggaatgtaa tccgccactt gtcctgtttg ataatagtgg aaattttttc
      961 caatcaattg ttctaattct tttacttcca tttgaataac cagctttcat tttttatttt
     1021 tctgcatgct ggaatttatg acggcatgcg tagatcgtaa atggaataac taagacgata
     1081 agataagaaa caatcagaat cgtttcataa atcccatgct gtccagctgc gatactagca
     1141 ggtggaacaa atgagataca aaaagcaaaa agtgaagtca aaagacctaa acaagcaacg
     1201 attactttta caacttttcc gcctggaacc tgataggctc tttcaagact gtcatcttta
     1261 aaaaccagct tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta gattacaaca
     1321 gtcaatgaca tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa tgtaaagacg
     1381 gctgcccaga ttgttgcaac gatcccattt gcgatcatca aatgagcagg aacaccgtgt
     1441 gtgttttctt ttgcaaaata ttcaggaagt aatccttctt ctgctgctac gtgaagtgct
     1501 tttgaaggag aggttaccca agaagcaatt tctccgatga cacctaaaca aacaagaatc
     1561 gcaagaattt tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag gaattctaac
     1621 gattgaatca cccctgtatt aagagacaaa ccatgttgag gtactgtagt agcgacggtc
     1681 aatccgccaa aagtatctaa aatgattgcc agaataacca atacaaacat tgctaatgga
     1741 taatcctttt tagggttttc catttcattt gcatgactgg cagatgtttc tacgcccata
     1801 tatgctaaaa taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa atcaggaatc
     1861 aatgttttca tagacagtat cgtttgtagt ggagcgcctg aagcgacata atggatccct
     1921 aagccaaaca agactaatgc tggcaacaag ataccagcta caaaaccgat ccgagcaatc
     1981 aatgaagtgt atttggttcc ttttaactgt gagagtaaaa tagcccagaa aataatcaat
     2041 aggattgcca atttcagata tttattgttt tgtatagcac caatacccgt tgcataggat
     2101 aatgcaccag ttaagaaata cagcatggtc aagtagccaa ctgttatctc aaaccattgg
     2161 aaaaagattg cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca agtatagaca
     2221 ccgccggttt cccttagtta aaaatattac tcttgttgat tactcaactc ttccaataaa
     2281 tcggtaatat atctcaattt cctgtattct ttcgttatcc tcattcgttg ttgcttcatg
     2341 tatgaggatt ttttctatca tggcatttaa taatggtgct gtcagttcct ttggtacgga
     2401 atattccttg attaactcaa tccacttttc agcacctatc atatcctgtt ccattttact
     2461 tagttcttct cttaggcttg ttatctgctg ttccagttct atctgttctt tttggtactt
     2521 gctggacaac atcacaaaat ttcgctctgt tatcttctca caggctctat cttcatacat
     2581 tttcgcaaat aaacggtcaa tctcattttg tctgttctcg gctttcttca atgtacttgc
     2641 ctttttcttc ttttcccgta ttctctctgc attgccagct ttctgtatct tgttcaatac
     2701 cttttcttcg tcctgctgta ctgccttagc ccaatactgc aagcgttcca atacggcttg
     2761 atacagcaca tcataacgaa tgtagtgcat agaacaatta cctttgccaa actgcccgta
     2821 gtaactgcaa gcataataac tgtaaggcgt tttatttgtc ttattcgttc caaatctcat
     2881 agaccaacca caatccgcac acttgacaag ccctgcaaaa atcggcgttg ccttttcctt
     2941 tgtctgtctg cgtcttgatt ttatctgctc ctgcacacga tagaacactt ccttgtcaat
     3001 aatcggctcg tgagtgtttt ctactcgaaa ccattcactt tcgggcttac gcactttctt
     3061 tttgctcttg aacgataccg tagactgcat attgtgaaca ctgtttccga tataaacctc
     3121 acttttcaat atcgccttga catgagcaat cgtccactca taacgcttac tttcgggttt
     3181 tccctcaaag atatgtgcaa aagtaccgta tcttgtaaaa tttagccatg acgctgtcgg
     3241 aactttttct gctcttaact gctttgtgat ttttgcactt ccgtaacctt gataggctag
     3301 ggaaaagatt ttttcaataa tccattttgt ttcatcatca accaacagtt ttcctttgat
     3361 gtcagggtgt ttcttatatc ctaacggagc ataagcaccg taatgagccc cctccgcaaa
     3421 ctttgtctta aatgctgatt tgactttacg gcttgtatct cttgccaccc attcattgat
     3481 gatgttctta aatggtgcaa tctcactttc gcctttttcg ctgtctacac cgtcatcaat
     3541 cgctatgtag cggacattat gactagggaa atacaattct gtatattgtc ctgtcataat
     3601 ataatttctg ccaagacggg aaaggtcttt cgttacaaca cagttgattt ttcctgcctc
     3661 tatatcctca atcattcttt gaaaactcgg tctgtcaaaa tttgttcccg accagccgtc
     3721 atcaatatat tcatcaatga cattcaaatg atgttctttt gcatataaac gcaacatact
     3781 tctctgtgtg gtaatgctgg aactttcgcc ctgtaattca tcatctcggc ttaacctaag
     3841 ataaagtgca gtattgtaaa tctgttgttt cattgtcagt tatcctcctt tataactaac
     3901 ccgtgtttac aatacctgct tgcaagtaaa gtataacacg ggtacttttt gtcattcaat
     3961 ctatttctta caactgcacc gattttatgc gattgtctgc tttgcttttt ccagcatgac
     4021 atcagttaga agttcttcca ttgttttgcc cttttcggaa aaatgctctt ttactacaat
     4081 ctttgtcttt cctctgttgc caatgccaca cttgccattt ttcttttgaa ttgtttttac
     4141 agcattatca ataacacata ccccctttct gtaaaatctt tctaagcaat tcaacggctt
     4201 ttgtcatagc ccacaggagt tccacctctg catagttctt atgtagccgt acccattgcg
     4261 tgcgacgctc ttaatgctca agctgtggct gtacgggagt atcattatct gacagtacag
     4321 gtcatggcga taaaagagga caaatctttt acaagaacac aaataggttt tcgctcgctc
     4381 ttgcagggga aaggtcgtgg cgtatttgtt tcaacggcta gctgtctgtc aaacgtattg
     4441 aatagcttta ttcagttgtc aaagaacgat tgaaagaaaa aaatcgtctt tcctatatat
     4501 cgcgttggaa aagaggagaa acgtaaccaa aatccaaaac tttttccatt attagtacaa
     4561 atggaacggt tcattttggc aatcaacggt tgattttctc ctttttcact actatctgta
     4621 cagcaaacgt ccatttgcta agtttaaatg gaaaaatttt aacttctttt catgcaccat
     4681 atagggatag aaaatgtcat tttgctaagt tgatgatgaa aaaagaatat tttccttttc
     4741 acaccctaac tacccaaacc ataccgttac tgccaacaat agaatgaaat attttctttg
     4801 caccatatag ggaacggaaa ccttaaattg ttaagttgag aacaaagtat tttcatcttt
     4861 tcacatcaac actcaactgc taatgcctgt ttgaatacaa aatgatgatg tttttcctct
     4921 tttcacagaa gactgtccac taatcacgaa tttccgtcat tttttcaaaa cttttttctt
     4981 ctttcacttc atatagggac agcaagcccc gatttaatga cctattttca aaaaaagttt
     5041 agatttcctt ttctcgcacc atatctgtac gcaagtag
//
LOCUS       DADXTG010000146         5000 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.149, whole genome shotgun sequence.
ACCESSION   DADXTG010000146 DADXTG010000000
VERSION     DADXTG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.149"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            410..1903
                     /locus_tag="IWI57_002409"
     CDS             410..1903
                     /locus_tag="IWI57_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBC2450510.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2038..2334
                     /locus_tag="IWI57_002410"
     CDS             2038..2334
                     /locus_tag="IWI57_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBC2450511.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(2358..3317)
                     /locus_tag="IWI57_002411"
     CDS             complement(2358..3317)
                     /locus_tag="IWI57_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2450512.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            3500..4351
                     /locus_tag="IWI57_002412"
     CDS             3500..4351
                     /locus_tag="IWI57_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450513.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4372..4655
                     /locus_tag="IWI57_002413"
                     /pseudo
     CDS             4372..4655
                     /locus_tag="IWI57_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1717 a          769 c          982 g         1532 t
ORIGIN      
        1 tagagtgaaa agaggtaaac ttaattctat agaaaccaca gataattcac tatttacaaa
       61 gtagcaaaag cagtggtact ctttaaacag aaaaattaca taaaaaaaag agacggaaat
      121 aattgatttt agcagaatca attatttctg aatcacatgg ttaggacatg tgttcacgct
      181 cgtctcaaga atatgtaagt agctaccaac tacttatatt aaaagttgat cggatacctt
      241 ttccgattgc ctttattcta ccaaaaaaag gcgagcaact caacagaaga acgtttattt
      301 agtgtactta aattaaatat tcttaaacac tgaagagaga acacatacta tggctgagat
      361 taaagctaaa ctgtgttctc tttttgtttg agaaaggacg ttacaatcga tgttgagcga
      421 aatttattca gctattctta aaaacggctt acgtacatac aaatatcgaa acagcaccat
      481 gaaacctgca ggtcatattg attccagtag gagtggtgct atctttgcgt accgtagcaa
      541 gcaattaatg aatgttgggc gtggtatggt cattacttcg gaagaagcta ttttagagaa
      601 cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc tttggtactt atgcggatga
      661 ttatcgaaca gtggttaaag gtcattctga gaaaaactta agtcaaatca acacatttgt
      721 agttgatatc gattcaaaag agaaccatca aggcgaaatt attttagcct gtttagatca
      781 agtgggctac atgccaacgc taattttaga atcagatcat ggttatcaag tttattttgt
      841 actaaagaca ccaggctatg tcacaaagaa aagcaatttc aaggtgatcg atgtagcaaa
      901 aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa ttgtccggag tggatctagg
      961 atgtaaccac ttcggaattg ctcgattccc acaagaaaat aatattgttt ttaaagaaat
     1021 gaactatcag tattcttttg ctgactggat tcaatggtcg atgaagcttg aatcagatca
     1081 aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt cctgagaaaa aagaaattcg
     1141 tcaagtagat gaaccgtggt tcgatatgtt acttcataaa gcaaacatcg tcggcggaga
     1201 aggacgtctt ggtcgaaata atgtgatttt cacgctttct ttagcttatt actcttcagg
     1261 ttatgcattg gatacttgcg aatacaatat gttcacattc aacgaacggt tacaggaacc
     1321 gctatctgaa gcagaattag taaagattgt acgtagtgct tattctggta attatcaagc
     1381 agcaagcaga gaattcatta ttgaattgtg ccagcaatgg gtttcaccaa ccttaaaaaa
     1441 taatgatctg ttcattcaac gacgtggctg gtggaaattt aaaaagccac gagatcaaag
     1501 aaaatatagt cataaaaatg aatggaaaga agatctattg gcttatctaa gcgaaaaaag
     1561 tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa ttggcttcac aactaacgat
     1621 gcccaagcga acacttgata agatcttaaa tgagttacga gaggagaata aagtcttcta
     1681 caaggtaaca cgaggaagaa atggcggaat gatcattgct tcagtcaaaa cgttgttcgc
     1741 gagggttatt caactaagta aagaaaagag actagattat tcaacaagga tcacagaggt
     1801 atttggactc aacgagagct tagtagaaga gacatttaga caattatcaa cccccttgaa
     1861 ccctcttaaa gaagtgaatc tttttgaagt agatacaggt tgaaaaataa aaggtgcaaa
     1921 ttgccttata caatatatct atgatacttc tgtaggtgtt gctccctttg cctagctacg
     1981 tgtaggtggt atacttataa ctgcgtatgc agtcttatgt atcatggagg aagttgtatg
     2041 tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt ttatcattgg tcgtttggga
     2101 ttatctgtgg aagtgttctg tgagctttat ggttttaagc aggggacttt gtcttcttgg
     2161 gttaccaggg aaaaaacagt tgcttctttg ccgattgagt ttcttcatgg gttgtcctta
     2221 gcttctgggg agactatgga tcaggtatat gattgtttgt gtgtgttaga gcaggagtat
     2281 attgagtata aaatagctaa cgagttaaga aaaaggaaga agtatatcca gtagtagttt
     2341 ttatttactc ttaattgtca aattaagtta gacaaatcat caatacttac gtaactcaaa
     2401 aatacttcca aaggtgttcg ataattcagt gattttctag gaatattatt tcgtttcgat
     2461 gcgacagatt gaataaaaga ttcatctact gaattgaaat ccatagattt caataaacca
     2521 tctctacgta acaatccgtt agaattctcg tttaggcctc tttgagacgg cgttcctgga
     2581 tcagcgaaat aaatggcaat atcattggca ttactgatct gtttccaatt tgaaaattcc
     2641 tttccacaat caaaagtgat ggatttgaat agatttttcg gtacagattc aaaccaatga
     2701 tttaatttgt tttcaatatc aatcgcttgt ctaccacacg gtttcaatgt gatgataact
     2761 tttgataatc gctcaactaa ggtaattaca gcacttttat gtttcaaacc tacgatagtg
     2821 tcaccttcaa ggtgaccaaa ctcatttgag aattggctat aatctttttc acgttcatga
     2881 atagagcgac ggaaagcttg ttttccacgt ctttcttgat gtccatttgg tttacgcttg
     2941 cctttcatcg gtaagtcaga aggattaaat agcccctttt tgaacatacg ataaatggta
     3001 cgagcagaac aacgaatagg aaacgctgca cgaccaacaa tcacatcggg tgtccatcct
     3061 tgaacaacct ttctttgaat atattctgat tgttcctcag gtaaaacaag cgggcgtcta
     3121 ccacagtttg atttgttttt cttatactgt tgatagtact ctaaggctga ttttccttgc
     3181 ttgaagaaca ggtatacttt atggatcgtt tgtcttgaac gattcaagaa atgcgcagtt
     3241 ttcgcaacag attgatttat tttgaaataa gactctatca ttacaagttc atccgttgta
     3301 agatgggtat aggtcatttg tactcactcc ttataattct ttggacggaa ctattttgag
     3361 tgtagcacaa atgacttttt tagttgtcta gcttaatttt acaatcggcg tacttaaaaa
     3421 attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt aaaggaaaga
     3481 atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct ggcttcctct
     3541 gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat taggaaaact
     3601 ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg gagtatttgg
     3661 aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca tttttacagt
     3721 gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag tcatgcaatt
     3781 taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt taattgttgt
     3841 aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa caacaaaagg
     3901 agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt atttgtttga
     3961 agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta aacgttcaat
     4021 tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt acaatgttga
     4081 ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta tccatgaatc
     4141 tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag caatagatcg
     4201 aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg gcatcggaaa
     4261 attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt ggaacagtag
     4321 tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa aatgattcct
     4381 ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa ggagcagtta
     4441 ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac gatgaacatt
     4501 gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt gattcgaaag
     4561 caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat gaatgggaac
     4621 aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta aagagcataa
     4681 ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta ttacgtttct
     4741 caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat agtgaaggat
     4801 agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta tcctttttaa
     4861 attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat cgtaagagaa
     4921 attgatcgaa gatgtgacga acggttctgt tgcaaagttt taaatctact atcaaataag
     4981 gtagaataat agaaaaagat
//
LOCUS       DADXTG010000147         4986 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.150, whole genome shotgun sequence.
ACCESSION   DADXTG010000147 DADXTG010000000
VERSION     DADXTG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.150"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWI57_002414"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWI57_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBC2450514.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWI57_002415"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWI57_002415"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBC2450515.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWI57_002416"
     CDS             2591..3376
                     /locus_tag="IWI57_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2450516.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWI57_002417"
     CDS             3389..4240
                     /locus_tag="IWI57_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBC2450517.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWI57_002418"
     CDS             complement(4364..4816)
                     /locus_tag="IWI57_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450518.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1687 a          815 c         1038 g         1446 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaa
//
LOCUS       DADXTG010000148         4926 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.151, whole genome shotgun sequence.
ACCESSION   DADXTG010000148 DADXTG010000000
VERSION     DADXTG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.151"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..682)
                     /locus_tag="IWI57_002419"
     CDS             complement(<1..682)
                     /locus_tag="IWI57_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBC2450519.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNG"
     gene            complement(727..1491)
                     /locus_tag="IWI57_002420"
     CDS             complement(727..1491)
                     /locus_tag="IWI57_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBC2450520.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1519..2217)
                     /locus_tag="IWI57_002421"
     CDS             complement(1519..2217)
                     /locus_tag="IWI57_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBC2450521.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2229..3008)
                     /locus_tag="IWI57_002422"
     CDS             complement(2229..3008)
                     /locus_tag="IWI57_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBC2450522.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3024..3968)
                     /locus_tag="IWI57_002423"
     CDS             complement(3024..3968)
                     /locus_tag="IWI57_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBC2450523.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4014..4793)
                     /locus_tag="IWI57_002424"
     CDS             complement(4014..4793)
                     /locus_tag="IWI57_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450524.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1285 a         1045 c          860 g         1736 t
ORIGIN      
        1 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
       61 aacttcgatg actaatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
      121 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
      181 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
      241 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
      301 ttttacctat tttagtgaca cggggatctt ctttcatctt gaacattgca ccttctattt
      361 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
      421 tatacatttt gaatgttttt ccatttttac caacacgtgt atggcaaaaa aatatcggac
      481 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
      541 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
      601 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
      661 ataataaatc ttcactttcc atactttccc tctctcctca tttccttttt atctacgaat
      721 aaagaactaa aataaaccaa acttcttctt ttttacctct tcatagactg gataactgat
      781 ttcatccccg ttgatcagat cctttgcgac ttgtttcatc tgtactactt tttccttacc
      841 aaagtcctta gcaatctgct catagcactc ttttaagtaa aaggtacgct tagagacccc
      901 atgggcatca gaagcagcca tctgcacgag attatgtttg accattgttt tggctgtttt
      961 ctgaatgtct ttgccaaata caccaacata acttggcgca gtaatttggg ccaaacatcc
     1021 catatctagg aacggaagca aatgatttgg gtcttctcga aaatgcgcgt tgcgttctgg
     1081 atggacgatc actggtgtct ttcctagttc taacaactca aaaaaaagcc attctgcata
     1141 taacggcact tccagtgtcg gaaattcgat cagaagatat gtatcatcta aatctgtaaa
     1201 taaaatttca tctcggtgga tgtcttcgat gagcgttccg gtaatacgta cttcttgtcc
     1261 ttccaataac gtcaaaggca actgtctttt ctctaattcg gcttgtaacg aagcgactag
     1321 agaaatgact tgtgattttt cattgctgta ttttccatta ttgtggtgtg gtgtgcataa
     1381 aatatgcgtg atgccttgct gaatcgcttt ttcagccatt gcaatacttg cttctaagtt
     1441 ttctgccccg tcatcgattc ctggtaggat atggcaatgt agatcgatca tttctcttgt
     1501 ctcctctctt cttattcctt aatttccgta gtaataacca gcatctttgc tatgttctgc
     1561 tccgttgtat acaacaccta aaactcgtgc ttgtaccatc tctagcagtt cttttgcttt
     1621 attcaatgat tctttacgtg atgtattttc acgtacgacc aataaagtgc catccgtttt
     1681 tgaagacatg atctgtgcat ctgttacggc tactactggt ggcatatcga atatcaccac
     1741 gtcaaataac tgtcttgctt cgtctaggat ctgatccatt cttggcgaac ttaacaattc
     1801 tgatgggttg ggtggcttag gtccactagg caaaatcgac aagttctcga ctggcgttcg
     1861 ttggattaca tctgctacac ttcctgatga actaagtgct gtacttaatc ccctggcatt
     1921 gttcagctgg aatgtcttat agacgacggg tttacgtaaa tctgcatcga ctagtagaac
     1981 tcgttgaccg gatttagcaa aaacgactgc aatgtttgct gcagtggtgg attttccttc
     2041 tccaggacct gatgaagtca cgacaatcgt tttgatttgc tgaccagctg cggaggcaaa
     2101 ctggatattc gtccggatcg tacgatattg ttcggcgatt ggtgaagatg ggtttgttaa
     2161 tgtgatcaaa ctgactgcat gagtttgtgc tgctttttgt ttctgtgttc gtgccatttt
     2221 tcttcctcct agactcttga gcggcttctg cgtgatgggg ctgttttatc gtttgtagct
     2281 tttggtcgtg caggagctgt tcctttttta cggataccag cgttcaattc tttcgctgac
     2341 atttctggaa ctgttcctaa gattgggaat cctaatgcat ccgtcacata tttttcatct
     2401 ttcactgtcc gatcaagtaa ttcgaataag aatgccagac ctacgcctac catcattcct
     2461 aatactaagc caatcactag attcaattta ttatttggcg aaacaggtgt cgtattggca
     2521 actgcacctg aaataatcga gattttatcg acattcatca cgtcttttgc gttctcttgg
     2581 aaaacttgtg aagttgtgtt agcgatctgt tgagcagtca ctgcattcgt gcttattgcc
     2641 tggatcgaga acatttgtga attttgtgac tgattgacgg agatcgcatc tttgatttgt
     2701 ccagcagtca ttttcacatt gtcttctgtt tctaaacgat ctttgacttc atttagtact
     2761 aaatcactga cgatcatatc tttatacgta ttgatcattt gcaggttcgt attgacatca
     2821 ttgacatttg ctgtgtctga ttgtggcagt gtcacgatca attgtgcttg tgaactgtat
     2881 tttggtgtca agatgaaaaa tgttacgatt cctgagatgg ccaagccagc aaacattgag
     2941 atgatgattg ttaccaagtg tttgcgtaat gtttcaaaaa tttcttccaa gttgattgtc
     3001 tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc ttttaattct
     3061 ttttttacac gatcaagttc ttgatcactg actttttgat aggagatatt atcttgcata
     3121 aagccttctc cttgcagctg atcttgcttg atcgtaccaa atgcctcccg ataatctaaa
     3181 gcgatttttt tcatttcatc aaagctcatg tctgttttca tattcgactc tagagcattc
     3241 aaaacggatt gatagttgct gacaccgttt aaactcaata ctttttttgc tacaccttct
     3301 acgatctttc tttgacgctc ttgtctacca tagtcaccat ttggatcttc ataacgcata
     3361 cgagaataag ataatgcctg atccccgttc aatgtgattt taccgattgt aaagtcatag
     3421 ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc atcaacaagt
     3481 tcctttagcc ctttcatatt gatagagaca tagtgatcga ttgggatgtc tacatatttt
     3541 tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct
     3601 tttgtgtcac gacctacgat gtcgacatac gtatcacgag gaatgctgac gatcgttgtc
     3661 tgtttgtctt caggactgac tatggcaacc atcatcgtat ctgatcttcc ttgatcgacc
     3721 cgacctaaat ctccagtatc aatccccatc aatagaacag agaaagattc ttgtttgtcc
     3781 agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgattcgta agttttctca
     3841 atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc taatactaat
     3901 acgataagaa ctgctaaaat aatcagaaat atttttttcc ctcttgattt tcttcttcgt
     3961 cttgccatag tttcctccgt ttgctaacct tgcttgaatc aacgttttaa ctgtcaataa
     4021 cttatattgc tatagcccat ttgctgagca acagtttgtg cttttgttgc gtatgctgcg
     4081 cttatttttt cattccggat ctgctctatt tgactgagcg ccgtcaaata ttgatcgtaa
     4141 gtagcggtgg cagtcacttg accatccttc aacatcgtat cgaataactg atctagttgc
     4201 gtgacttgcg cgatctgagt atcagcgaat cctaaatatt gcataacaac cgttttccaa
     4261 ggactatctt taaaaaagct catattttca cgtacacgag cgacatctgc acttgtcacc
     4321 ttttctttga tgatgacatc gtctacagct tgctgccagt ttgatacatt tttagtaaac
     4381 aaatcgttga cagctgtctg gatcttcagt tttgattctg cactatctaa ctgtgtcacg
     4441 attgctttct tctcatcttg gattgcaaca gcttctttag gcaattctga ttctttgatg
     4501 tcgtaatcat ctgctgaaag tttgacatag gatagcttat ttgacaaact tgtgattgtt
     4561 tcttctgtta tttcagggtt tagaaaaatt ttttcttttg tactatacag cttgtctact
     4621 tcttttgata aagaagtcaa ggtgtctttt tgattggtga tggcagattc tgcataattg
     4681 atccgttgct gatgttgccg ctgattattg atatggtaac caaacaaaca gatcgcaata
     4741 gcacctgcac ctaccgccac agcagtcaat gacttgacga atggcttttc catagattca
     4801 ctcctaagta ttgatgatag ataaattaag atataattca cttacataaa aatgacagct
     4861 ttttatttta aaaaacataa ttaaactaat atattcacag aaatatttgt aaacaagttt
     4921 tcccta
//
LOCUS       DADXTG010000149         4909 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.152, whole genome shotgun sequence.
ACCESSION   DADXTG010000149 DADXTG010000000
VERSION     DADXTG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.152"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..1738)
                     /locus_tag="IWI57_002425"
     CDS             complement(38..1738)
                     /locus_tag="IWI57_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450525.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2245..3405
                     /locus_tag="IWI57_002426"
     CDS             2245..3405
                     /locus_tag="IWI57_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBC2450526.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3421..3864
                     /locus_tag="IWI57_002427"
     CDS             3421..3864
                     /locus_tag="IWI57_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBC2450527.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4347..4877)
                     /locus_tag="IWI57_002428"
     CDS             complement(4347..4877)
                     /locus_tag="IWI57_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBC2450528.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
BASE COUNT         1639 a          872 c          924 g         1474 t
ORIGIN      
        1 ggagagtttt ttgatcggat gtatttatcc cttttgccta agcacgtaaa agcttgtatc
       61 tatgataaat aaataaacat agcaaggtaa accagccaat caaagtaaga gaaagaatga
      121 aatagaaata gattggcgtt tgataatgaa gcgtgagctc gttgattcct ttttgctgct
      181 tcacgattgg ggtcccgata tctgttactt cataatcagt caatgttttt ccattttgct
      241 gtagcacagt ccgatcgtag acgataacag ggatatgaac agttttgtcc ccagtgtttt
      301 tccacttgat tgtcaaaaga ccgcctggat gtgtttttat aaaagttgtt tcgttttcta
      361 gtatttcttc gttataccgg tcgtatttat tgtcttttgt ctccttatag attggcagat
      421 agtcaggtgt ggttttttgg aaaacgaaca agctggtaga catatctttg ctgaagaatg
      481 ttttccttgc ttcttccggc gtatcgaata gataagtatg tcgactgaca aatgtttcat
      541 tttcccactt ttccagatgc tggtataagt tctggatcgt ctgtgctgaa aaaataagaa
      601 tgccaaatat acttaaaaga cggaaccact tttgttcaga ccactggttc agtgaaaggg
      661 caaggtaaag tagaagcaat agcgtaaatg gcacgaaaaa tcgaaatgga aactggatca
      721 aactaataaa aggcacattt ttttcgatca atgtcgtcca tggaatcaag ctagtagaaa
      781 gaaaaaagaa aacaagactt gtgtacaaca cgattttttg gaataaggtg ttcttttgtt
      841 gaaagaaaat aaaaagcaaa aaacctgata atatcaatat aggaaaggcc ttagggtaat
      901 ggagccaata actgccatcc agatctatcg ccttttgact catttctctg ttaacaaagg
      961 gaggaagaat cttattccca ctgtaaacaa tagctaatcc tgcccaaata tttgctgtca
     1021 gaacaaaata aagcaagacc gacttaatca gttgaagcag tgcttttcct ttttcagtct
     1081 ggttaaaaaa gtaataaaga taaaaaggga tatacatcaa agctaacatg atcgaagaca
     1141 gtaaatggat ctgtgtcata agtcccactg agacagcgac tttgataaca gggaactttt
     1201 tatgaaggac gaaatcagca atagggatca tacataaagg ataaaaacaa gtcccccagc
     1261 tagtgaatcc ttggttgata gaccagtatt gaatagaata cgtactcatg aataaaacgg
     1321 ctactggtac acttacccaa tttcgaacat tgacatatct cagtaaaatc agcatagaaa
     1381 atcctgaaat aagataaaga atgaaatttg aaagaatctg ataatgaaac cagctacctg
     1441 caagcaacac gatcaatcct tgtaaataag cgaaaaatgg gccgtaaacg gcattcacga
     1501 tcctgccact ttgctggaat ccataaaggg aaggaaaata atggaaatcc ccatatttaa
     1561 tttgttgcgc ggcatcatag aaccgattgt aatggaatgg ggcatcagca cccaaaatta
     1621 gtttatgtgt gaataaatga ggcatgagaa atgccaaagc aatgaaaaaa attgctaaaa
     1681 atgggccata ttttttcaat tgtcgaactc ctttgcaaac tttagataaa ttcatcatat
     1741 atcctatgcg agaggtaggc aagaaattag aaaattcatg aaaatattta tgaattttac
     1801 aagaaaaaca taagtgaaag ttctaaggag agaggatgcg acataagttt tgttgcatct
     1861 ccttgttttg aaacagaatt tttcttaata ctcgaaacaa agtagctttt tcatgacctc
     1921 tcggtaaacg gtgttcaatc agctgcttct cggtaataag ctcactagcc tagaaaatat
     1981 gagaagctat tttctgacta gcgctcttat tactcaaagc ataacgcagt tttcacaacc
     2041 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccaac ccaacaaaaa
     2101 atatgagtag ctattttttt gggctgtact tattacttga tgcaggacag ctttttcaca
     2161 acctcatcgg ctttcatttt ctattttttt cctgaaccca tataataaaa gaaagaacaa
     2221 aaaaacttgc aagggaggac acatgtgaag cagagtaagc aaaaacagtc tcgtcgactt
     2281 ttgtttcgtt tgtttttatt aatatgtatc gtcgtagggt tactgatcgc tttatatcca
     2341 ttttatgtgg attctttaaa tagttttatt gatcaaaaac gtatcgaaca agtacaaaaa
     2401 cggaccgcag ctgaaattga agtgcagcga aagaaaatgg aggagaaaaa ccaacgatta
     2461 actgaccaag gattcaatcc tggtactgat cctttcaatg aaaagaacag aaatgaaagt
     2521 acaacatcca gccaattaga ggaatggctg atcggaagta tcaatatacc taagatccaa
     2581 ttaaatattt cactttatga tcggctcaat ggtaggatcc ttgaaaatgg agcaggcgtc
     2641 ttgcaaggaa cctctttccc gcttggtgga aattcgacgc attcagtgat ctctgctcac
     2701 agcggtttac ctaatcgacg gttatttacc gaattagatc gtttagaaaa cggagacacg
     2761 tttattttga ctgttttggg ggagaaattg gcttatcaag ttaaaaatat acaagtggta
     2821 ctgccagatg acacgtctgt tctaacgatt tcagaaggag aagacttagt taccttgctt
     2881 acatgtacgc cgtatatgat caacacgcat cgtttgctag tgacaggaca tcggattcct
     2941 tataacgaat cggtaaaaaa agaagaagga aaaggaaatc aggaaagaac catgcggcaa
     3001 tggtttattc ttgcaggaac gataatagct gtagtcatac tgttagtgtt tatcggacga
     3061 ctaatctatc agtatagatt aagtaaaaaa gtgcttgatt tttcatttat tattagtgat
     3121 tcgtcaggga aaccggtaaa tggtagttca ttcattttga aacataagaa aaaaacactt
     3181 actagaaatg gtgtgccgtt ttcagtacaa agcgaccatc atgggaaagt caagctggat
     3241 caattgcctg gtggaactta tcgaatcgtt tcagctgatg atcctaaagt tgctgtttct
     3301 tttggtatta gaaaagtaaa acaagagaag atatactttt ttgaggggcg aaaacttgta
     3361 aaagagcttc aaaaaaatgg tttttatttt aaaatcaatg aataagtaag gagagaaaaa
     3421 atgatccgca caacaacaaa attgtctgcc aaagaactgg aatctgttct atcgatttgg
     3481 ctgcatgcta acttggattc tcatgaattc attccatcaa cttattggga gaatcaagta
     3541 gaatctgtta ggaaagcttt accagatgca gtgatttact tagcagaaat agatcaagaa
     3601 attattggtt ttgctggggt gattgatcag tttatcgcag gaatctttgt gaaaagggaa
     3661 taccggcaac aaagaatcgg tacgcaacta atgaaaaaaa taaaagaaac caataaaaca
     3721 ctgactttga cagtctatca aaaaaatcaa tcagctattc atttctatct ttcacaaggt
     3781 tttacaattc aaacacagca aaaagatgaa gcagtaaatg aggaggaact tgtgatgaaa
     3841 tggaatcaaa acgctgattt ttgatgaaca gaataacgaa ataatgctcc cagcctaatt
     3901 ttttgctggg agcattattt cattttgaag tcactaattt aactgtgtga atatgcagtt
     3961 gttacaatct ttgataaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
     4021 gcaaaatatg aggaactatt tttccatttg tttctctaat actcaggtca aaacgctgat
     4081 ttcacaacct cttgataaac ggtgttcaaa cggctgcttc tcggtaataa gcccactagc
     4141 ctagaaaata tgaaaagcta ttttctaact agcgttctta ttactcaaag cataacgctg
     4201 atttcacaac ctcttgataa acggtgttca atcagctgac cctcggtatt agctcaaaaa
     4261 acggcaaaac atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct
     4321 gatttcacaa cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat
     4381 tttccgctac ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca
     4441 ttgtctatac ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt
     4501 ggataagttt catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa
     4561 gtaacagtga tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt
     4621 ttcttttcat ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg
     4681 attttgaata cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg
     4741 cctttgacca ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat
     4801 tcatattccc aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat
     4861 tctttaaatt ctttcatgtt taagcatctc cttataaatt aaaacatgt
//
LOCUS       DADXTG010000150         4823 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.153, whole genome shotgun sequence.
ACCESSION   DADXTG010000150 DADXTG010000000
VERSION     DADXTG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.153"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..500)
                     /gene="dfrF"
                     /locus_tag="IWI57_002429"
     CDS             complement(27..500)
                     /gene="dfrF"
                     /locus_tag="IWI57_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBC2450529.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(919..1137)
                     /locus_tag="IWI57_002430"
                     /pseudo
     CDS             complement(919..1137)
                     /locus_tag="IWI57_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1168..2382)
                     /locus_tag="IWI57_002431"
     CDS             complement(1168..2382)
                     /locus_tag="IWI57_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBC2450530.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(2723..2965)
                     /locus_tag="IWI57_002432"
     CDS             complement(2723..2965)
                     /locus_tag="IWI57_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBC2450531.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(2997..3554)
                     /locus_tag="IWI57_002433"
     CDS             complement(2997..3554)
                     /locus_tag="IWI57_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBC2450532.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(3567..3755)
                     /locus_tag="IWI57_002434"
     CDS             complement(3567..3755)
                     /locus_tag="IWI57_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBC2450533.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(3768..4334)
                     /gene="amaP"
                     /locus_tag="IWI57_002435"
     CDS             complement(3768..4334)
                     /gene="amaP"
                     /locus_tag="IWI57_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBC2450534.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1443 a         1006 c          733 g         1641 t
ORIGIN      
        1 caccctaaat ccaaaattta ctcaattcat tttttcctta cataaaatgt tctcgtatat
       61 ttcacttcct caccaagtgt ttcccctatc aaaacttcaa aatcattgat atcaaattct
      121 ggaaagaatg tatctccatc ttcaatattt aaatctactt ctgtgatata cattttatct
      181 actatttgca aagcttcctt aaatagtcca tatccaccag atatgtatac atctcgtcct
      241 ttagccaata ataatgcatc ttctaatgat ttaactgaaa ctaaattatc tccttgatac
      301 tctgttgtgg tggaaacaac aatattcatt ctattaggca acggatgacc gatttcttca
      361 taagactttc gccccataat aaccacatta cccgttgtta actctctaaa ttgcttttgt
      421 tctcccttta ttttccatgg tatattacca ttcttgccta taacattatt ctttgacctc
      481 gcaacaatca aacctatcat ctatttcatc ctccataata tgatgtcgta cctgtgattc
      541 catcaagtgc accacatctt aacaaactca aaattatctc gaactaacca gctcgacaaa
      601 ttcctttttt gtcttcatct gcattatatc atattgttat aagcattgcc atttcttttc
      661 tgcttttgaa aagttactta tcagcctatg ttttgtaaag ggtgatattc agcatttctc
      721 acgcaacaag cccttgacaa accatagact aataagtttt aaggtaatca agcagaaatg
      781 aaatagtctg ctgtttcacg gcaacaaaaa agagatacca acacacttat gtagtatctc
      841 ttagtctagt agcaatattc ttttgtttgc aagcaggtaa acatgggctg tttcttaata
      901 tctttttgtt tgggaaacac ctttttccca tcctttcacc gttgccatct ctgctgcgca
      961 caatgccaca gggataaacc atagcaaacc gcctaataac aagaaaaata ctaagctgaa
     1021 gcctgaggtc gcgaatgtgg gatattcata taatgaaacg accattgaga tggtcatagc
     1081 caaaaagcca aagaacgtta atttttttgc aggaattttc gttttgcttt gttgcatgaa
     1141 tcttcctctt ttctatttga atttgtttca attgagtttg ttgccaaaga agaaaaagtc
     1201 caatccttct tcttgttctt cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt
     1261 taaaatcact gttgtcagca cataggcata cacggcagta atgatgacac caatcacttg
     1321 aatccctaag aagtaccagc tgcttggatt cgttagtaaa tggctgttag atgcaaagat
     1381 ccctatcaga atcgtacctg tgaatccgcc catgccatga acaccccaca catcaagtgc
     1441 atcatcccat tgtttctttt tacgaaaatc aacagcagca tgacagacga tacccgcaat
     1501 gatcccaatc agcatggcac tttttgcttc tacataaccg gcacaaggcg taatggctgc
     1561 caatccagca acggatcctg ttagtacgtc gacaaaatca aagccatttc cttttacttt
     1621 tgcaatgatc agccaagtaa tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc
     1681 attgactgct tgttcatctg ctcgaagtgc accaccggaa ttaaaaccga accagccaaa
     1741 ccaaagcagt cctgtaccga tagcagctgc cataagactg ctatgctgac ttctttctcc
     1801 taaaggaatc tttctttgac caagtgtgag gatacatgct aaagagccga atccagctgt
     1861 cgtgtggatg actgctccac cagcgaaatc tctaaatccc atctgatcca aaaatccgcc
     1921 accccatatc cagtggcaaa cagggaaata aatcagcaga ttccaaaaaa taacaagtat
     1981 aatatagcct tttaaattca atctccctgc aaaagctcct gtcatcaaag gaacggtaat
     2041 cacgataaac atcagttggt aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa
     2101 catgttgggg aagcttgtaa catgtctcat caaaaagaaa tctgccgggt ttccaataac
     2161 accgccgata tcccgtccaa aggccaatcc gaatccacca aatacccaca taatcgttgt
     2221 tatcccaacg gagagaaaag attgagacat gatcgttagt atattattct tccgtgctaa
     2281 tccgccgtaa aaaaaagcta gaccaggagt catcaggcaa accattgctg tacataaaat
     2341 catgaatgct gtatctcctg tattgatagg taatgtattc atttttttcg cctcctttct
     2401 acgattacga gtataaaaga aggtgtagtt gtttttttct tctgtttttt tgaattaata
     2461 cttgcattta taaaagacag acaattaata aaagcttgat gtgttgtgct ctttttcgtt
     2521 tttcacacaa ataaagacaa aaaataaacg gtcttctttt tatagaaaac cgtttatttt
     2581 cgtctttttt tatttttaaa gatatttttt cataaggaat aaagaatact tcatataaaa
     2641 tatataaaaa agaaccgcag cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa
     2701 cataagtttt tcttcaattt gcttataatt tacgtgcaaa gaatgaaacg actaatacta
     2761 aaatgattgc accgatcaat gatggaatga tagccattcc tgcaattgtt ggtccccaag
     2821 ttcctagtaa agcttgtcca attgctgaac ctactaaacc agcaatgatg tttgcgatga
     2881 tccccattga tgatccttta ttcgtgattg ctcctgcaat tgctccaatg ataccgccga
     2941 cgattaatgc ccaaataaat cccattttaa attcctccta actgacttct tacttcttat
     3001 tgtacacgtg gttcgttatt ttctttgact ttttgtgcac ctttgcctac agcagatttt
     3061 gctttatctg tttggcgtga agcaaattgt cctgtggatt ttgctgcttc tgttactttg
     3121 tcttgtactg tttcttgatc tttttggtat tcttcttgtg ttttgatgtc tgcaacattc
     3181 acattcactt cgataacatc taaatgtgtc atattgttta cttcagtact gatcaattct
     3241 ttgatttggt tgtaaatatc ttcgatgtct ttaccgtatt ctacgatcac atctagatcc
     3301 acagctactt gtttcttacc tacttctgta ttgatacctg ctgttacgtt atctgtgttg
     3361 actaattttt cagccatatt tgagaaaaat ccgccatcta atgtcaatag accatctaca
     3421 tctttcaatg cataaccgat gattttttgg atgactttgt cttcataagt caattcacca
     3481 ttcatcatgc ggttttctcc tttttcattt gccaaaactg atccatttgt tgtattcatg
     3541 tttttgtttt ccatctttgt ttcctcctat gatttaaaaa attgatcaac tattcctgac
     3601 tgtttcaaat aaaaaccgat tcctgcgcca attgctgtca tgattatgat caacaatgtc
     3661 ttgaaaaatc caatcgttaa ccataaaaca gctagcagta gtccaagacc cccaaagata
     3721 atagggagct tgtatgctga aaatagttct ttcattattt ggccctccta ttccactcga
     3781 gagtgcttgt agtctcttgt atcttcttgt tcttcataac cgctgtatgt gactgccact
     3841 ttgaccgttt cttttgttcc gagtacttta gttacttctt cttcgatatc ttgcatcaat
     3901 acacctgttt tccctactaa tgaagaagtt cgtttcaagt ctccgttgac atggatatgg
     3961 attcgatttt tcgttgaacg tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt
     4021 aaatgggaac gtaccaaccc ttcgatcgct tttttgtcga ttgttagttt gccatcttcc
     4081 cgtttcataa cgaaagttcc tcttgacttt ggatagaaaa gaacgactag caagatgata
     4141 atagcaagaa cggcaaatac tacagctgcc cagaaaatat attgttgcat gtagtaattc
     4201 gttaaagtaa agaagcgaaa tcgttccaat tgttcaggca taatatacgg cgcattgatc
     4261 aacgcaatcg ctagaaatac tgaaagtagc agtaaactca tgataacacc gattattttt
     4321 aaccccttat tcattttcca cctcctataa acttattttt accctgtaaa taactctacc
     4381 gacaatcaca gtatagatag cgaacagtta gcatagcatc agtaaataaa gcgctaccaa
     4441 acattgctgg atcataacgg cgttttattg ccagtctgat actgaaataa caattgctta
     4501 cttattctct atttactttt gattgctggc tatacttaaa ggatattatt caagattaaa
     4561 aattgcaaag attatgctca gttattatta caagattcta tttatttatt aaattttttc
     4621 ggagaattaa aaaatacaat aaataacttt agttacttta atgcgttctt actttatttt
     4681 tatattatca tttttcttat tcacttcttt ggtttatact attcttataa ataaaaacga
     4741 ggtatgtcct atgatgaaaa gattgtacta ttcattgatc ataacgattg gctatttgat
     4801 tgtatcaaat ctaggaaata tgg
//
LOCUS       DADXTG010000151         4626 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.154, whole genome shotgun sequence.
ACCESSION   DADXTG010000151 DADXTG010000000
VERSION     DADXTG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.154"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IWI57_002436"
     CDS             complement(61..2424)
                     /locus_tag="IWI57_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBC2450535.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2741..3928
                     /locus_tag="IWI57_002437"
     CDS             2741..3928
                     /locus_tag="IWI57_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBC2450536.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            4346..4567
                     /locus_tag="IWI57_002438"
     CDS             4346..4567
                     /locus_tag="IWI57_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBC2450537.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT         1317 a          912 c          889 g         1508 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgattacgt
     2641 ccatataatt gtgtaaaagt gaagcatcaa aaaagccatt gatctcagtt aggagtacaa
     2701 tgttttttgc agaaaacata ctcgaatagg actgatatca atgactcaag tacattttac
     2761 ttttgaaagc gaagaaattc aagcaattat aaacgaaagc ggtgcgaatg ataccgcaaa
     2821 aacacttatg actatcatgt ttaatcagct gatggaagaa caacgaaatc aatatattca
     2881 ggcaaatgcc tatgaacgtt cagaagaacg gcaatcacaa agaaatggtt actacgatcg
     2941 ttcgtttaca actcgaattg gtacgttaga acttcatgtt ccacgaacca gagacggtaa
     3001 gtttagtcct actatctttg aaagatatca gcgaagcgaa aaagctttga tcgctgccat
     3061 gattgaaatg gtcatttctg gtgtctccac tcgcaaagta actaaaacag ttgaactgct
     3121 aacggatggt gcgactgttt ctaaatcatt cgtttcaaat ctgatgaaac agttggatcc
     3181 atttgttttt gagtggagaa accggagcct agaaggctcg gaatatccat ttttcatgtg
     3241 tgatgccctg tatatgaaag taagggaaaa tcatcgtatt gtctcaaaag gtgtatatat
     3301 tggtatcggc attgattctg atggacgacg tacgattctt ggttttgatg ttcaagacgg
     3361 cgaatcggaa gataattggg ataccgtttt tcaatcgttc gttcaacgag gtttgttcgg
     3421 tgtaaaactg gtcatttctg atgcacacaa agggttagtt aaggctgttc gcaagaactt
     3481 tttaggtgcg agctggcaaa gatgccaagc ccactttttg agaaatattt ttgataagct
     3541 gccaaagaaa gtttcttctg atgtaaaaga cgagttgaag agtatcttta aagcctctga
     3601 acttgaattg acacgtgaaa gaaaagagca cttcttggaa aaatatggtt gtgattcaaa
     3661 attaagtgct gcttgcgaca ttttagaaaa cggctttgaa gatgctattc aaattttgtc
     3721 ttttcctgaa aatatccgcc gaaggattcg tacaaccaat gtgttggaac ggttgaacga
     3781 agagattcgc cggagagaac gagtgattcg gatttttcca aatatcaatt ccatcactcg
     3841 gataattgga acacttttga tggaaaaaga cactgagtgg ctggcttctc cacgaaaata
     3901 tttagaattt aattcgaata acatttagca ccatgaacct gtgcgagtct cttctttctg
     3961 aggagagaca tcaagagtcg ctgcgctact gctcctgacc tctctcctca gaaagaaagg
     4021 catggcagtt aggttcacaa cgcaaaacat tgtgcttttg atgaggttga acttttacac
     4081 aagattgtgg acttgacttt catttcgctc ctcatcatgc ataatttgct gaatgacgct
     4141 aggccagtta acacattgtt ggaaaactgg ggctggatcg agcaattcct ttcctgattg
     4201 atccgtttta tgtaaaagtc tctgttatct ttgtcaatat gtgaattggt atcccagcta
     4261 ctatgctaat ttctacaggg attatccaaa atttggatcg tgaccaaatc gaagcggcgt
     4321 aatcggaaat atcaataact tcaacgtgat ttttctttta actgtcaacg gaccgacaaa
     4381 ctcaaacttt tatggtgcag gttccactga tcttctagtt acttggctat acctgactgt
     4441 cactaaaata gattacaatc tagcttccgt tatcggtatc ttgatcttta tctatatgca
     4501 aaaatttata tactatagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt
     4561 tggttagctg acacgctacc agactaaagt catggaactc ttaggtaata cgtcttaatg
     4621 aaaaca
//
LOCUS       DADXTG010000152         4595 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.155, whole genome shotgun sequence.
ACCESSION   DADXTG010000152 DADXTG010000000
VERSION     DADXTG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.155"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..708
                     /locus_tag="IWI57_002439"
     CDS             <1..708
                     /locus_tag="IWI57_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBC2450538.1"
                     /translation="ILKSSEDLSQHDLLFSKFAADYIQELIKKANVVALSWGDTVYKV
                     IDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQKAAERYESSYLTLSIPLYVINET
                     ARELMALEPSLANTLATASHADMLISGIGTLSSIDSVEPWNKHRKILFPSMTDTVGFL
                     YGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGICKSKFKAESCLGALNGNFFTDL
                     FLDEKTAWKILSLIKKE"
     gene            complement(1005..1553)
                     /locus_tag="IWI57_002440"
     CDS             complement(1005..1553)
                     /locus_tag="IWI57_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450539.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1554..2411)
                     /locus_tag="IWI57_002441"
     CDS             complement(1554..2411)
                     /locus_tag="IWI57_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBC2450540.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2697..2984)
                     /locus_tag="IWI57_002442"
     CDS             complement(2697..2984)
                     /locus_tag="IWI57_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBC2450541.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2974..3303)
                     /locus_tag="IWI57_002443"
     CDS             complement(2974..3303)
                     /locus_tag="IWI57_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBC2450542.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3380..3652)
                     /locus_tag="IWI57_002444"
     CDS             complement(3380..3652)
                     /locus_tag="IWI57_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBC2450543.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3652..3915)
                     /locus_tag="IWI57_002445"
     CDS             complement(3652..3915)
                     /locus_tag="IWI57_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBC2450544.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(4021..4437)
                     /locus_tag="IWI57_002446"
     CDS             complement(4021..4437)
                     /locus_tag="IWI57_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450545.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4443..>4595)
                     /locus_tag="IWI57_002447"
     CDS             complement(4443..>4595)
                     /locus_tag="IWI57_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBC2450546.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1347 a          884 c          637 g         1727 t
ORIGIN      
        1 atattaaaaa gtagtgaaga cttatcacaa cacgatttat tattttcaaa atttgctgcc
       61 gattacattc aagaattaat taagaaagcc aatgtggttg cactatcttg gggagatacc
      121 gtttacaaag tgattgatca gtttaaaatg ggtgctcgtg aagaattaat ttttactcaa
      181 ttcattggcg aaatcggaaa atataattcc ttagctggct ccacacgatt agttcaaaaa
      241 gctgctgaac gttatgagtc ttcttatttg actctcagta ttcctttata tgtcatcaat
      301 gaaactgcac gtgaactaat ggctctagaa ccttctttag ctaatacctt agcaaccgct
      361 tcgcatgcag atatgttaat ttctggaatt ggcaccttat cctctattga ttctgtcgaa
      421 ccctggaaca agcatcgtaa aatcctattt ccaagcatga cggatactgt tggatttctt
      481 tacggaagac ccttcaatat caatggtgaa ttcatcaaag catctgagga taagactttt
      541 ggtctttctt taaaagaaat tttcgatatt ccaaaacgaa ttggtatttg taaaagtaag
      601 ttcaaagcgg agagttgttt aggcgccttg aatggtaatt tttttacaga tttatttttg
      661 gatgaaaaaa ctgcttggaa aatactttct ttgataaaaa aagagtgagt catgtaaaat
      721 cctccaaaaa atttagattt ttggtactac ttttttggag ggcagtacaa tgattcactc
      781 tttttattgg atatcaaaat cccttttttt gagaagcttg tcttgatatt ttttgaactt
      841 ttccaagcca tactccatta cttttcaaga tttaatttct ttgtattcac tgaataattt
      901 ccacgttttt tcgttaatgt tacttcttat taattttatt gtacttctca tgcaatagga
      961 tcatatatcc ttctaaatct tttttatgct ataaaaaggg ggttctatct aactttatta
     1021 ttcattcccc accctttgcg cagtgagtct tcggtatctt gttttatctg actggtagtt
     1081 cttattattt ctccacggtc atctaattct ggaacaattg gatatttttc tctgaacaag
     1141 tctagttctt tcaccacgtc attaaacaaa tcttgcttat cttcttgcgt aagattctgt
     1201 ttaatatact catctaaagc ttttctaagc aattcatctc ttgtcatatt agtctcatac
     1261 ttttctacta aaactctgga agtcgcattt aacaaagtcg acagttttaa aggatgcttg
     1321 gtggaataaa ccttatcaaa ctttctgatt ggtgcgccaa ctttttttgt tcttcctcta
     1381 ctctgtcttt caacattgga ggaagttttt atttcttgaa cttggtgcac ttctaattgc
     1441 ttattttcag atggtgtaat tgcttgctgc tttttctttg cttctgcttc tttttttatt
     1501 ttggcgagtc ctttgttgtt cccaaaaata tcaagtcctt tgatgtcagc caattactcc
     1561 acctccgcat accaattctc gtgttcttcc aattctttca aaatactgat aaataattcg
     1621 tggcttttgc gatcccattg ttttaagaga cctttctttg tcttctccat cgtaatacct
     1681 gatccgtcgt atcgttttaa gcgcgcattt ttgagtacaa catttttaat caaatgttcc
     1741 ccataaatct cttgggcttg tttatacatt tcctgatcta ctgcgtcacc gctttccaac
     1801 atgaaaggaa taacaccagc aacatataaa tttgtatcaa attctttcac aaaaaagtcc
     1861 atatactcaa gatattgcat cgcatttctc atcgatttta cttgggtctg taagacgacg
     1921 attgcccagt ccgcaatgta catcgcggat ttactatata cagaaataga aggtgggaca
     1981 tctaaataga tgacgtcaaa ctcatcaaat aatggttcta gcttttcttt catatatttg
     2041 aattgggata atgaatcatt atattggtct ttgatccaat catcatacat ttcaaaggac
     2101 atatctgctg gaagtatata gaggttattg attactttat agacagcctc tttaggtgga
     2161 agattgttta tcaacacgtc agtcacagta ttttcgtaaa caatcggttc accttctccg
     2221 cctccttctt cgttagtgaa gttcatatgc gttaagttca tcacatcaga agtatttcct
     2281 tgcatatcca agtcaattat caaggttctt tttccttcat taactgctga ttcaaaccct
     2341 aacatttgcg cgcacgtact tttacctacg ccacctttaa aattaccaat tacatatttc
     2401 ttcgctacca cgttcctacc tcttttcttt tagcaatatt catttgatac tattataaca
     2461 tatttatttt tagttactac caaaaaaaag atatatatgt actaccttat aattaataag
     2521 taaaaggtag tacataaata aggaaaagtt gaaatatatt gatttatagc tttcctcaaa
     2581 gtaatgacgt tatttaattt atcgagaagc agagaatact cgcgacttta gtcatgtaga
     2641 cgaatcagat tttcgataaa aaagaagccg tccttcttaa gaacgacaac aaatcactag
     2701 attaattttt tcaactgttc ataaaagttt tcatgcgttc cacataacaa tactggaata
     2761 accttgtttt ctataatctc gtaagctact ctatatgttg tgcccgcata tttgaaccca
     2821 ctagaccaaa tacctgataa atccccagtt ttttgttcac ccttatatgg atcaatcgca
     2881 atttcatcat aaattaaatt caaaaatttt tctttcaatg gcttctcttt tagctttttt
     2941 aaatacttaa tgacatgttt tgaaggaacg atttcataag ccaatttctg ttgccaactc
     3001 ctctttagtc attactttag aattagtaag tgtatcttca gcaatatctc taaaggaaga
     3061 atgcattttg ttttttctat tcttaaattc tacaagaatc tcatctttat cgtagccttc
     3121 tgcaattata tcagacaaaa tatcttcgct aaaatcataa aattcttttt gtggttcaac
     3181 aaattcataa agaataccat tctctaccaa ctttgcttca acttcgactc cattaggaac
     3241 attaaaatct ttaggtactg tcaacatgat tgaattgcct tgttttcttg tttttgtaat
     3301 catttaaatc cctcatttct aacagtatgt aattacttag taaatacatt ataccacgag
     3361 ataatgtatt tacaagtaat tactgtgact cgtaatgcgt ccttgctgaa taaatttcaa
     3421 ccacgtgttc atcttcatct accttataaa caacccggtg ttgtctattc aatcttctag
     3481 aatacctacc ttcatatgta ggttttaact tctcaaatga ttgggtaggg agatagggat
     3541 cttcctttaa agtctgaata accttctcaa attgaggttt caaattagac tgtttgattt
     3601 tctttaaatc agctttagcc gagtttttaa tcatcacaga atagttattc attataagtt
     3661 atcccaatca atttcatcaa cattagtaaa tccactatca tctttttctc gcttacgaac
     3721 aacacccaaa gtgcctattt gctcaagtaa tagcgtttct ttgattgctt tcaaatcatc
     3781 caaaccaata agcaccgcat tattttcact gcgctcacta atgatttcaa tttccgtatg
     3841 attctcattt acatctttca acaacttata aaaatttttc cgtgctgagc taggatttaa
     3901 gactgttttt gccatatcta ctccccctct aatttatatc tccattctaa cgtacttaat
     3961 taagtacgta aattcgattg ttttgtttaa attggagtac caaaacagca ttgtaaagtg
     4021 ttatccgttt cctttatttt tattaacaag ttttatcaca ggcttcatat atttgattat
     4081 aacttcatct tctacgtaga tatctgttat cattacgaca tctgtactat ttcgattgag
     4141 tactttcact tgatctccag cattgatttt ccctctaaat cttttaggaa tgaaccaact
     4201 atacatatga ttagcatcac caaaatgtgc ttttatcgca ttctcaggat ataacttatt
     4261 caatagttca ttctttttaa ttttcaaaac ttcaaatcta tacttatcat catctgctaa
     4321 tctaaataaa tcttctaaac gcatgttttc taactgtaag ttttgaaaat cttccggtat
     4381 aaattttcgg ttctgttgcg aagtttgaaa atcaaacgcg ccctctctga actccaaata
     4441 tcttaggctg ttattcccat taataccttg atttcagtag acaccgaaaa gctgaagagc
     4501 gttccatttc ttcggttctt tttatatatt cctcgaatgg tctccatgcc cttaatcgtg
     4561 gaagaggctg tacggagact ttgataaaat ttatt
//
LOCUS       DADXTG010000153         4593 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.156, whole genome shotgun sequence.
ACCESSION   DADXTG010000153 DADXTG010000000
VERSION     DADXTG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.156"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            102..377
                     /gene="rpsP"
                     /locus_tag="IWI57_002448"
     CDS             102..377
                     /gene="rpsP"
                     /locus_tag="IWI57_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBC2450547.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            389..640
                     /locus_tag="IWI57_002449"
     CDS             389..640
                     /locus_tag="IWI57_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBC2450548.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            765..1556
                     /locus_tag="IWI57_002450"
     CDS             765..1556
                     /locus_tag="IWI57_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450549.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1726..2247
                     /gene="rimM"
                     /locus_tag="IWI57_002451"
     CDS             1726..2247
                     /gene="rimM"
                     /locus_tag="IWI57_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBC2450550.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2237..2998
                     /gene="trmD"
                     /locus_tag="IWI57_002452"
     CDS             2237..2998
                     /gene="trmD"
                     /locus_tag="IWI57_002452"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBC2450551.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3134..3481
                     /gene="rplS"
                     /locus_tag="IWI57_002453"
     CDS             3134..3481
                     /gene="rplS"
                     /locus_tag="IWI57_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBC2450552.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3739..4032
                     /locus_tag="IWI57_002454"
     CDS             3739..4032
                     /locus_tag="IWI57_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBC2450553.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(4120..4233)
                     /locus_tag="IWI57_002455"
     CDS             complement(4120..4233)
                     /locus_tag="IWI57_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HBC2450554.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
BASE COUNT         1621 a          789 c          919 g         1264 t
ORIGIN      
        1 aatcgaattt aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac
       61 actacatctt gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt
      121 taaaacgtat gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt
      181 ctcctcgtga tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg
      241 cagaagtagt tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt
      301 ctgacactgt acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta
      361 aattctcaaa gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat
      421 cgttcgtccg ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga
      481 cttttacgag tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca
      541 agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa
      601 aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa
      661 ttttcgagtg cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac
      721 aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt
      781 tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca
      841 atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca
      901 aaacggcttc caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt
      961 tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt
     1021 caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca
     1081 gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa
     1141 cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat
     1201 ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc
     1261 aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc
     1321 gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt
     1381 tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg
     1441 atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca
     1501 ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc
     1561 agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca
     1621 cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc
     1681 acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg
     1741 aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca
     1801 acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga
     1861 aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg
     1921 agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa
     1981 gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc
     2041 ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca
     2101 ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat
     2161 atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca
     2221 gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg
     2281 tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat
     2341 ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg
     2401 cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga
     2461 aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt
     2521 cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca
     2581 ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga
     2641 ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct
     2701 tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt
     2761 attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt
     2821 attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag
     2881 gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa
     2941 atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact
     3001 ttttctaaaa tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa
     3061 aagcacttaa ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa
     3121 ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga
     3181 catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac
     3241 tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag
     3301 cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt
     3361 gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa
     3421 attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta
     3481 atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt ttcttagcat
     3541 gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag aataatggat
     3601 tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa tatctttctt
     3661 aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag tattcttaga
     3721 atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat gaaagtttta
     3781 gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa aaagaaatac
     3841 atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac agaaacagag
     3901 gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca aagacgttat
     3961 ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca gatgatggga
     4021 attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat gcgatattgg
     4081 ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa agccaagaac
     4141 gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag ttaaataagc
     4201 aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat gcgttacatg
     4261 aataaagata attctctttt atttgtacga ataaatacca aaacgtacct cctaaaatta
     4321 gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa caatatatat
     4381 gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag aaaattgaaa
     4441 atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc agctaattga
     4501 acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct cttttatgtt
     4561 caaagataac aaatatttaa aacaaagaaa aat
//
LOCUS       DADXTG010000154         4577 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.157, whole genome shotgun sequence.
ACCESSION   DADXTG010000154 DADXTG010000000
VERSION     DADXTG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.157"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            591..1502
                     /locus_tag="IWI57_002456"
     CDS             591..1502
                     /locus_tag="IWI57_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HBC2450555.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1989..2255
                     /locus_tag="IWI57_002457"
     CDS             1989..2255
                     /locus_tag="IWI57_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450556.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2327..2668
                     /locus_tag="IWI57_002458"
     CDS             2327..2668
                     /locus_tag="IWI57_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450557.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2723..3142
                     /gene="ssb"
                     /locus_tag="IWI57_002459"
     CDS             2723..3142
                     /gene="ssb"
                     /locus_tag="IWI57_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBC2450558.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3219..3515
                     /locus_tag="IWI57_002460"
     CDS             3219..3515
                     /locus_tag="IWI57_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450559.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3530..3961
                     /locus_tag="IWI57_002461"
     CDS             3530..3961
                     /locus_tag="IWI57_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBC2450560.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4143..4448
                     /locus_tag="IWI57_002462"
     CDS             4143..4448
                     /locus_tag="IWI57_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450561.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1683 a          721 c          969 g         1204 t
ORIGIN      
        1 ttccaggttt ttgaccattt tctctcaacg tggaaaaacc gccacttatg agagaaaatg
       61 gtcaaaaacc taagcgggaa gtctccgaat aacataccaa gcgccgacag ccaacagata
      121 aagaatacac tactaaaaat agtagtagca taaaatttaa aagggaaaaa atcaacccct
      181 accccagtct tggggtctca aaactaactc ccccctaacc ccctctttca gcctcttttg
      241 aaagaggggg agaatcaacc ccacggccaa cgccccagtg ttcttggggc tgtccagaac
      301 agaaacttgc gagagagtgg gggtaaaaca agtgtagagg aaacttgaag acttgttcga
      361 acagaaactt gcgagagagc cggagtaaaa caagtgtaga ggaaacttga agacttgttc
      421 gaacagaaac ttgcgagaga gttggagtaa aacaagtgta gaggaaactt gaagacttgt
      481 tcgaacacaa acttacaaga gaggggagta aagcagttgc agaggaatca ggaagatttg
      541 ttcgaacaga aacttgcaag aaagcattat taaattgaag gtgatgaaat atgaagtatt
      601 tattgcatgg ctattttatt gattattcta ctcgcaatag tagtacttta gcgattctat
      661 gtttgcttca tgaattgaga gtggttacta gaaaacagct atacaaacta ttgaattttg
      721 acagatcaat tagtaaacaa ggagtaacga atgctttagt gagattatat gaaaaacagt
      781 taatcgatcg aaaaaaatat ggaacagaaa tgtattatta tttgacgaaa gagggacatc
      841 aaagcatagg tgggtattat acgttgccga aagttcctga atataatttg aatcaccatt
      901 tacaaattaa caattatttg attaaaggaa tggcattggc aaaagaccac ccacatttaa
      961 aacggattat ttcagaaaga cgtaccgtct atgaaacgaa ggatttaaat cctaagacaa
     1021 aaggaagaaa attttttgtg ccagatttta tttttcgttt tcgagacaaa gaaaatcatg
     1081 agatcaattg gtcctttgaa attgagctaa cgttgaaaac acgtcgtcgc taccgtgaag
     1141 cgatttttcc gaaatatatt cgtgagttgc gaacacgtcc tgaatcacat ttaatttatg
     1201 tgacacctag tggcgcaatt caagaagaac ttcatgcgtt taagcgtatt tttttcaagc
     1261 gcgaaactga aaaagatgcg agtcaacaag aattgtttga gcggttgcat attttttcag
     1321 ctttagagtt tgaagaggga ttaaaagcat tattagagaa tgatccagca gtcaattggc
     1381 ggacagtaga acgagaaaaa atttatccga atggcgctgt tcaaagtcgt ttgaatttat
     1441 ttgaagaatc aactgaaaat gaggaaaaac caaaagtgaa gtttacgcct gaagtattgt
     1501 aaaagagggt gttcgcttcg ctcttttttt gtgccagttt gctggcaccg ttcagacgca
     1561 agcgtctgaa ccggctcgtt tttaggaagg actaacttgt ttgatttatc cgttgtcaaa
     1621 gaagtggacg ttcgagagtc cctaatcgta tagcagtcat tcggcgagta taacctaaaa
     1681 cataacctgc caattgccaa gggtaaaatg cacgctgcgc gcccttggca atcgtcagaa
     1741 catgttttcg agccggttat gccgaacgac cgctatacga aaagggcctc taaacagaag
     1801 tccactttga tccttgacaa ctgaaaccca aaatcaaaca agcaggcctt cgtctaaaaa
     1861 cgagccgaat gaagaccttg ggggttgcac ccccaaaccc ccgtgccccc gacacactac
     1921 cgaaaaggtg ggggtgggtg gcgaaaaaat caaataaaac attctattca agaaaggaag
     1981 aaacaaccat gacaactaaa aacgagcaac tgaaagaaca ggcaagaaaa gagttgcaag
     2041 aaagaggttt gattattgag gggagctttg aaggagattt tgaaacctat attggttgtt
     2101 atgcaagacc gataaacaaa cctacagcct tagacccaat gaacgaacaa gaagtaaaag
     2161 aacaagaaaa acatgcagtg aatggttttc cgcaagattt cactgaatgg tatgagtggg
     2221 aaattaacaa cgggaagctt gaaaacttct tataaaagtc agatgacgtg caaagagaat
     2281 gattctcttt gtacggtaaa aatataaaga aagaaggaaa aagaaaatgg caaaaaacac
     2341 accttatcaa gtaggagaca ttttttatag tagttggggc tacgaacaaa cgaacgtgac
     2401 tttttggcaa attgtgaagt taacagaaaa aacagcatgg tttcagccta ttcaaaagaa
     2461 aacggtcgag cattattcgt ccatgagtgg aaaagttgtt ccaatgaaag acaagtttat
     2521 agagtatcct ttagcaaaaa ctaaagacag tattgtaaaa aaacgaattg atccagaaac
     2581 ctatcctgat atgttatgtg gttatcgtag ttgggacacg tttcatagat ataaaaatca
     2641 agctgtttat gaaagtagtt attattaagg ggatagtttc ccctttcaaa aataataaat
     2701 agaagaatgg agagaaaaaa tcatgatgaa ttcagtgaat ttagtaggac ggttaacaaa
     2761 agaagtggat ttaaaataca caccaaaagg caatgcgaca ggcacattta ttttagctgt
     2821 taatcgaaat tatacgaatg caaacggcga gcgtgaagca gattatatcc gctgtgtcat
     2881 ttggcggaaa gctgcggaaa ctttagcaaa atatactgga aaaggaagtt tgattggcat
     2941 taatggacgt cttcaaacga ggagctatca gaatcagcaa ggacagacca tgtatgttac
     3001 ggaagttcta gtgacagatt tctatttgtt agagagtaaa gaaatcaatg agcaacggtc
     3061 aaaatcaatg gcgaataatc aaaataattc ttcaattcca ggaaatgaga ttcaaatttc
     3121 agatagttat ttgccctttt aagtgaaaaa taattttgtt ctatacctga tttcttcagg
     3181 tatagaacaa aaaataaata gagatgaggc gattttttat gaaaatcatt gacagttacg
     3241 atcaacgaac catgcaatat ttgggctcgt ttgattatac agatgaaaat attattaatt
     3301 atgttgcaag tttacctcct tatcaaagcg ttcgcttggt agattttgca agtgatgaat
     3361 tggtattaac aacgatgggc aactttttag attatgtcgg agacaaaaaa tggttggaaa
     3421 aaattttgtc taaattgatt gcgaaacaaa caggagaaga acccataaat gcgattcaat
     3481 tgttcaatcg atatgaacaa gaaaacaatg attaaagagg agagaaaaaa tgaaaagatt
     3541 aaaacgaagt atttttattg tgctagcatt gattgatttt gttgggacag gttgtgaagc
     3601 aattgacgtt caaaaagctc ccttatcgga aataaaacaa gcaataacaa aacaagatga
     3661 agaacaaccg ctaaatacac aagtagaaat agtgaatcaa aataaaccga gtttttctga
     3721 agaagatttt tcgttagaaa atggaaccag gcaatttttt tcagatttgg atcgtttaaa
     3781 tcgggtaggt gtagcaagtg cacttttgca taaaagcagg atgccaacag aagaacgaga
     3841 ccgattatac attaagccaa ctggctggaa acaaaagaaa atgaagaatg gcgattatct
     3901 ttataatcgt tgtcatttga ttggctatca acgtgcgaca agtttcccca taagtatgta
     3961 atacgaaacg ggaggattaa tcttaagatt aatcacaact aacctatttg acagttggaa
     4021 tgaaagctat catgtctgct gaatcaactg gtctgatact cctacatgca ataaagtcta
     4081 ctaggaagtt attgtatgaa gctaggtaaa ggcggcagaa caatttctaa gtgtggaatc
     4141 atatgaagtt ggatatgccg ggacgctata aattactcag ggtgagactg tttttaatta
     4201 aactgacgaa cttgcgaatg tacgggtcta taaccgactt atattcgaaa gatataagat
     4261 tgcgaatgca aagaacatta tcgaaaagta agaatgttgg tcatgaaatt cgagatggaa
     4321 gactagcggt gtatctgtca agagttggta aaacatgttt gattgattca gatgagagtg
     4381 ggaatgcgac aaaacgtttt acagaagaaa tagagcaact agcagaactg aatatacgat
     4441 atttttaaat actttagcgg agaataaaaa aatcctctac agaaaactac ggagggcaac
     4501 atgtgctatt gtttcaacaa tacattttta atttttacca taacaaaaaa gtacttgaaa
     4561 aaaaacagaa atatgtt
//
LOCUS       DADXTG010000155         4576 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.158, whole genome shotgun sequence.
ACCESSION   DADXTG010000155 DADXTG010000000
VERSION     DADXTG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.158"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(52..714)
                     /locus_tag="IWI57_002463"
     CDS             complement(52..714)
                     /locus_tag="IWI57_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2450562.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            898..2544
                     /locus_tag="IWI57_002464"
     CDS             898..2544
                     /locus_tag="IWI57_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBC2450563.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2535..3617
                     /gene="uxuA"
                     /locus_tag="IWI57_002465"
     CDS             2535..3617
                     /gene="uxuA"
                     /locus_tag="IWI57_002465"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBC2450564.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3725..>4576)
                     /locus_tag="IWI57_002466"
     CDS             complement(3725..>4576)
                     /locus_tag="IWI57_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBC2450565.1"
                     /translation="KDVQKEFTEYEYIDFFIDLSVDILRPNLPICREQRILVNILARL
                     SITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFAR
                     NKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKE
                     NVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFM
                     ENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSK
                     KENQVTS"
BASE COUNT         1460 a          794 c          900 g         1422 t
ORIGIN      
        1 tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg
       61 gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg
      121 caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc
      181 ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg
      241 gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc
      301 aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt
      361 tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt
      421 gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg
      481 agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt
      541 atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat
      601 agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata
      661 gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat
      721 tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt
      781 atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt
      841 tattgcataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg
      901 attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata
      961 cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat
     1021 ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat
     1081 ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa
     1141 agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag
     1201 ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct
     1261 gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt
     1321 gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt
     1381 gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg
     1441 ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat
     1501 ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa
     1561 aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg
     1621 tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg
     1681 ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt
     1741 gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat
     1801 ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa
     1861 gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg
     1921 ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg
     1981 ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt
     2041 gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct
     2101 aatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag
     2161 acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca
     2221 ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc
     2281 atcccgagtc cagacccact attgacagac cttcagcata agctttccga tattcaactg
     2341 ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa
     2401 atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc
     2461 aagatggtag caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat
     2521 ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta
     2581 ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata
     2641 taccagtagg agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt
     2701 ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg
     2761 gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag
     2821 aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg
     2881 accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc
     2941 cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc
     3001 cgggatggga acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag
     3061 tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat
     3121 gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg
     3181 gattagctcg aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca
     3241 gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg
     3301 tatatgggat tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa
     3361 atattaaatt tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt
     3421 cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc
     3481 gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg
     3541 atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca
     3601 aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc
     3661 aaattcttga tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt
     3721 tggtctagga agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta
     3781 attcaaagat atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga
     3841 ttccttttaa aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc
     3901 tgataacgat gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa
     3961 aaatttgttc tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca
     4021 caccaatcac aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa
     4081 ccatccattg cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc
     4141 tgaaatccat ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt
     4201 gataaagcat gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat
     4261 aaagtttatt tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca
     4321 aatcaaatac agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt
     4381 ttgctttttt taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga
     4441 tagaaagtcg agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag
     4501 gtcgcaaaat atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt
     4561 ctttttgtac gtcttt
//
LOCUS       DADXTG010000156         4480 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.159, whole genome shotgun sequence.
ACCESSION   DADXTG010000156 DADXTG010000000
VERSION     DADXTG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.159"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(229..732)
                     /locus_tag="IWI57_002467"
     CDS             complement(229..732)
                     /locus_tag="IWI57_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBC2450566.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1091..2116
                     /locus_tag="IWI57_002468"
     CDS             1091..2116
                     /locus_tag="IWI57_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBC2450567.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2439..3317
                     /locus_tag="IWI57_002469"
     CDS             2439..3317
                     /locus_tag="IWI57_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBC2450568.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3793..3909
                     /locus_tag="IWI57_002470"
     CDS             3793..3909
                     /locus_tag="IWI57_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBC2450569.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1470 a          724 c          814 g         1472 t
ORIGIN      
        1 tattgactag gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa ccctttttgt
       61 taaataaggg gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct gcaataatat
      121 agtagattag agatgaaaat agattttttt aaaccaatga ctgaataaaa agaccgtcat
      181 agatacacat tggaatgtgt ctattacggt ctttttttac gaacgatttt attgaagacg
      241 gacagttcgg gtcaaaaata aaaaagctac gatcagaaat aagaataaaa tgtatcccaa
      301 aataatggct agacggtcta acttatttgt cgatagatgt ttgtttagaa gttgataggc
      361 ccataaacct atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt ccgctacgcc
      421 aataccgaat aggaattgaa tcaattctgc caagaagctt acaaggaaaa taatccctaa
      481 ttttttagaa aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa aaggaaaaaa
      541 gacaatcaag ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag aaaaagtaga
      601 aaaagggacg agattgacat tgctgtaaga ataagtgaga aattgtggaa gattaaatga
      661 tagcttgaat aaaatcaacc ataatagaaa gaataaataa atccctaata aaaattttga
      721 tacataccgc atagacatct cctccattta aaaagtatac catcgaaaac cgctgaaaaa
      781 tcataaaaaa gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat tagaagcttt
      841 tttttgttgg gaatccggtt tctttaacaa agctaagata gcgagttgct tggaaaagtt
      901 gctatctttt ttctgtcaaa acgactcgga atgttcgttt tttgttctaa tttctccaag
      961 ttgtttactt tttgatacta agtttatgaa aaatcaagaa atagttgtta tactaggtaa
     1021 tgctgacaaa cgggattggc atttcatctt ggttcagctt tttttccaat tactaggaag
     1081 gagggataga ttgttcaaga cttattttcg tttcaataaa atacaagata atccttttcg
     1141 tatcatgaat ctgatgctac ttatctttat tgtattatgg ttaggttaca taaatcatga
     1201 ttttttgatt tcaagttttt catcattagg gatctttact tttttctatt atcaaaacat
     1261 accgatgaaa tatttgctga aacgtatgtt tctgatcggt tgcggactgt tggttgcttt
     1321 cgttttgggg atcttatcga cttatgtttt atggcttgaa ccatttgccg tagctttggt
     1381 tgctttttcc agcagattcg tgttgcgatt attccatatt tctaaaccag gtggcttgtt
     1441 ttttgcgatg ctttctgcca tgggaacgag catgcagctg ccaatcgctc aattaccgat
     1501 cgtctcactt tattttttta tgggtgtcgt ttttgccctg attgcggcag tgattacaaa
     1561 attactggat tcacggcctg agcagacaat tgaaaaagca actttaaaag aaagatttca
     1621 cgaagagcct ttagtgatca ttgattctgt tttctacagc gctgccttat ttctttccgt
     1681 ttatgtcagt cacggcttga atctgcataa tccttattgg ttgaccttgt catgtgcatc
     1741 gattttgcta gcagaaaact tggatgcgat gaagcatcga caagtccaat atctgattgg
     1801 atcgatgggt gggttatgtg tgtcggcatt cttgtcattt gtgccgttta cgcagttgca
     1861 gaccattttt ctgatcactt ttctatatgg aatcgcacaa tttctagttg ctagaaatta
     1921 tgcagttgct aatatttttc ttaatccgat ggcgttgatg ctatctacac ttattcgtgg
     1981 agcatacttg atttctttga tcgaatatcg ttttttaggg atcgtcatag gaagtttcat
     2041 cggtttaggt gttgcgtggg taatgacggt tggtttgcaa cattatttaa cggttgttcg
     2101 tcaaaaatta gaatagaagg acaaatatat gtgccaaaag aggttatgac aagatttttg
     2161 tcacaacctc taattcaagg tgttcaaaag ccagattctc agcattaagt caaattcaca
     2221 caaaaatgtg gaaaggaact ttcaaaaaaa ttttatattt attctatcaa aaaattttgt
     2281 ttatgatggt aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac
     2341 agatattctt tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt
     2401 tataccatat ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa
     2461 ggggaaattt gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc
     2521 ttcccaaagt cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt
     2581 ttgagcaata tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa
     2641 tagacaaagt ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt
     2701 ctcaaagttg gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa
     2761 caatgaatgc ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct
     2821 ttgaccgtgg tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat
     2881 tggagtacga aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc
     2941 agtgcctttc tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata
     3001 gttacgaaag tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga
     3061 tgtacacaga tacttggtta ctatcaccca atcttactaa atggttgaaa aaagagtcaa
     3121 aaatctgtct ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg
     3181 gagtcccttg gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga
     3241 gtttacagcg acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag
     3301 gtattcttac ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt
     3361 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc
     3421 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta
     3481 tttcttggag ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag
     3541 tacgttcaat aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc
     3601 ttttttaggt agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag
     3661 ctagtctgat cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa
     3721 acacaaatcg taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag
     3781 tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca
     3841 aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg
     3901 aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt
     3961 ttcaataatc ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta
     4021 acatatgata aattggtaga atctttcttt tcaaattgat accattccct aattccagtt
     4081 atatagttaa ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat
     4141 ttttcaattc cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa
     4201 cacctttgtt cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa
     4261 aggttttgcg aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat
     4321 aaccattatc gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac
     4381 tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt
     4441 taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat
//
LOCUS       DADXTG010000157         4464 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.160, whole genome shotgun sequence.
ACCESSION   DADXTG010000157 DADXTG010000000
VERSION     DADXTG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.160"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWI57_002471"
     CDS             574..1008
                     /locus_tag="IWI57_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450570.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2301..3224
                     /locus_tag="IWI57_002472"
     CDS             2301..3224
                     /locus_tag="IWI57_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBC2450571.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3240..3779
                     /locus_tag="IWI57_002473"
     CDS             3240..3779
                     /locus_tag="IWI57_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBC2450572.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          668 c          758 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaattatata agaataggtt taagagtgct taaatcgaat
     1741 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     1801 gttttaagaa caagataagg gatttttata ctaggactgt cttaaaatgt cttaaacgaa
     1861 cgctcagagc ttctcaaagg gttccttatg aacggctttt agcaaaggag ccggaggcga
     1921 ctgcgcagta taatgtgaga gcagagcgat cacacctttt tagcgaaccg tagggagcaa
     1981 ggtttggaat tggcagtttt gataaattcg cgaacctgtt tagagaaaaa aggagtgatt
     2041 ttttaggcgg gaaaattagc ctcccctctt tttatatctc tttttatatc tctttttata
     2101 tctcttttat gtcggctgtg tccttactct cccaagcaat tcagagtttt ttcaagtagg
     2161 ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttct acgcttttca
     2221 agtaggtttt ccgacattat tgagtatttt tataaagtat gttactttta tttcatactt
     2281 tataaaagta ggagaagctt atggcaaatg aaattgtaaa atatgaaaat agattaaatc
     2341 atattcctct gagaaaattt aatagtcgtg aaatgaactt atttttttca attgcttcac
     2401 gtgtacgtga aaaaggtgta gatgagatta ttttttcatt tcacgatttg aaaaaactaa
     2461 gcggttatac agaacgtggg gaacaattta taaaagatct tagtagcacg tatgacaaat
     2521 tattaagtat taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca
     2581 caaaatatag cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta
     2641 aaggactttt taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt
     2701 tggatagcac atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct
     2761 ggactgaatt ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga
     2821 atagtccgtc aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct
     2881 tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg
     2941 gttatcgttt tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac
     3001 aagaagattt acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag
     3061 aaaaatggcg cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt
     3121 ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac
     3181 tcaaacaaga tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta
     3241 tggcagataa aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta
     3301 acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg
     3361 tgattaacga aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact
     3421 taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag
     3481 tttccgaatt aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc
     3541 tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac
     3601 tcactctaca agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata
     3661 cagaagaaac tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac
     3721 aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa
     3781 attttctaga agttagttcg gatagtagag gaaaaagttg tactacctga tggggagatc
     3841 actcaaattt ttatgagaaa gaaaaaatag tttacaactg taaacaatca atgtttatag
     3901 ttgtaaacta tcaaactata tggtgactta ttgcttaaaa taggttgaaa tctcactcaa
     3961 tttttggtgg aaaaacaaaa taaattctaa tcgattttgt tgaaaaagtc ataaaaaaac
     4021 aaatgtgatt ttaacaaaat ttcaaaaaaa tcgtttgaaa tttcgtttat tttttttgag
     4081 aattttgatc atcgtagggt cattgtagaa tcattatgag atcatcgtgg gatcatcgaa
     4141 taaagtatgg gaaaggcttg cggtgcttgg tattaagggc attctccgcc tcccaggttc
     4201 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     4261 tgggaggcgg agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DADXTG010000158         4435 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.161, whole genome shotgun sequence.
ACCESSION   DADXTG010000158 DADXTG010000000
VERSION     DADXTG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.161"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(57..668)
                     /locus_tag="IWI57_002474"
     CDS             complement(57..668)
                     /locus_tag="IWI57_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBC2450573.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(791..916)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(998..1714)
                     /locus_tag="IWI57_002475"
     CDS             complement(998..1714)
                     /locus_tag="IWI57_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBC2450574.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1720..2289)
                     /gene="scpB"
                     /locus_tag="IWI57_002476"
     CDS             complement(1720..2289)
                     /gene="scpB"
                     /locus_tag="IWI57_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBC2450575.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2273..3070)
                     /locus_tag="IWI57_002477"
     CDS             complement(2273..3070)
                     /locus_tag="IWI57_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBC2450576.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3040..3411)
                     /locus_tag="IWI57_002478"
     CDS             complement(3040..3411)
                     /locus_tag="IWI57_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBC2450577.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3430..4317)
                     /gene="xerD"
                     /locus_tag="IWI57_002479"
     CDS             complement(3430..4317)
                     /gene="xerD"
                     /locus_tag="IWI57_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBC2450578.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1201 a          970 c          776 g         1488 t
ORIGIN      
        1 gtcttgtatt tttttccaaa aaaaaagacc aagaaatttc ttgatctttt tgatatttat
       61 ttgactgctg tatgacgttg ttcgaactga tgttgttttc gacttaacca tggaagaagt
      121 tgcgcatgca gcactaaaaa gacagcactc acgatgaatc ctttgatcaa gttgaaagga
      181 acgatcccaa tcagtacata gtttgctaaa gacatgccca gcatctgtgt tgcgttcagc
      241 cctaaaaaca ttaagtaaag aggcgtaata acgaaataat ttgcgatact cataaaaata
      301 gttagagcga tggttcctgt aatgacacct aaaaatttgt ttcttccttt atgctgtttc
      361 tttacactaa agaaataata aatcgggaat gtaaagatcg ttgttgataa gaaactagca
      421 gtgtctccta ccaaattatc cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc
      481 aagaacgctg ttgcaactcc ggccgctgga ccgaataaga acatgctgat caataccgag
      541 atatcactaa aatcgatctt caaaaaacta aaagccggca tgatcggaaa agcaaaatac
      601 tgtaaaacta atgcgatcgc agcaaacatc gctactgcaa ccattttctg tacacgggtg
      661 ttcttcattt gaaatcctcc tgttaggact cctcttcagc gaactttttg acgtttctct
      721 ccaaatgaaa aaactctatt ttccagggaa aatagagtta gagttatttg atcaagcgtc
      781 gtcaaataag ctctatcttc tttatccaga ctatactgtc ggtatcggaa tttcaccgat
      841 tcagctattt ttttgcaaaa taggtcgtgg actataacca ccggtcggga atttcaccct
      901 gcccctgaag acgaacctta tataattttt gtttacatat tcattataca tatctattca
      961 aaaaacgcaa catctaacaa ttataaaaag taagtgttca ttttgagtgg ttcagcaaac
     1021 tgctgacttc ttttttgttc agttcacggt aatccccagg acgtaggcct tgtaaagtca
     1081 gatctccgta tttttcacgt tttagttttt gcacaggaag accaactgct tgaagcatat
     1141 ttttcacttg atgattacgt ccttcgtgga tcgtcaattc cacgatactg tggtttgtct
     1201 taggatccac tgataaaatt tggaatttag ctggagatgt ttttcgtcct tcgatcttta
     1261 ttccttttgt caaaggtgca agcatcgatt ttgtagcgat cccttttacc tttgcaacgt
     1321 aaactttatc cacttcatgt tttggatgtg tcaggcgctg agcaaaatct ccatcatttg
     1381 tcagtagcaa gatccccgaa gtatcgtaat ccagtcgacc tacaggatag atacgttctt
     1441 ttaccatcgg caaaagatcc gtaacaactt ttcttccttt gtcatctgca acagcagaaa
     1501 taactccttt aggtttgtac aataaatagt aaccgtattc ttcttgataa attgggactt
     1561 catccacttc cacagtgtca tgtttcccaa cttttatccc taattccctt acaacttgtc
     1621 cgtttacttt tacacgtcct gcaaggatga attcttctgc ttttcgacga gaagtgatgc
     1681 cagcgtgggc aattactttc tgcaatctct ccattacaat tattctcctt tattcattcc
     1741 atcaaagaat agatctggtg acacgtcttc ttcaagtgaa atttccattt cttgtgtatc
     1801 aggcagttct tctaatgatt tcaaaccaaa ataatctaag aagtagccag ttgttccata
     1861 taatatcggc cgacctggtc cttctactct acctttttct tcaatcagtt tatgggcagc
     1921 tagacgctgt accgctcctg aaacctgaac tcctcgaatc tgctcgattt cagctcttgt
     1981 gattggctgt ttataagcga tgattgctaa ggtttcgacg gcggcttgag ataatgtgtt
     2041 tgatgtccct tgcgcatatc gcttaagtaa gggagccagc tcttttttag tcgttaatac
     2101 ataatggctt tccgtttcaa aaatatgcaa agctgagtgc gagttttgag tgtaagattc
     2161 ttttaattct ttcactaaac ttgtgacttc tgcttcgctg agatttaaca gtccgcttaa
     2221 ttcttcatta ctgatccctt cctcgccaac aataaaaaga agggcttcaa gttcacttaa
     2281 tttcgtcatg ttcggtatct cctactgctg gataaagcat aattgattca tagttttctt
     2341 tttgttgcgc aatgatctgc ccttttttca tcaattctag caaagccata aaagtagtga
     2401 caacttcact tttagaaggt gactcaaaaa aagaatcgaa agtacagcca tgcgcatttc
     2461 tttttatacg ctcttcgata aataccattc gttcttcgat cgttgtgtca tcgggcgtga
     2521 tcgttttttc cactttttga gaaaatttct tacgttctaa gattttctgc atggcacgga
     2581 tcaaatcgat ttttttgact tgtcctcgct tcaacggctt ttcagcatcc gctaacccat
     2641 cgatgttcat aggttctttt gtgtaataaa gacttcgttc ctctgctttt tctgtcagtc
     2701 tttcagctgc atatttatat ttacggtatt ctaaaagctg agtgaccaat tcttctcgag
     2761 gatcttcttc ccataattct tcatcgtctg tgccgaattc ttggacagga agcaatgtct
     2821 tgcttttgat tgccatcaat gtagcagcca tcactaagta ttctcctgca acagctagct
     2881 cgagtgtttt cattgtatgg atataattca tatattgctc cgtgatcgta gcgatcggta
     2941 tatcatatat atcgatttcc atttgctgaa tcagatgaag cagcagatcg agaggaccct
     3001 cgaatacatc taatttaata ttgatctccg tcaactcatt cactctttct tctttttctt
     3061 tgctagccat tttccaatgt aaggctctgt gatttcagtt gcacggatgg agacatccgg
     3121 gtacttttcc tgtaattcat ctaacatttg aaagcccata ttctcaccgc gataagacgg
     3181 attcaaagaa atgtcatgga tgatcagctg atcgttcgtt gcccagtaaa taccaataat
     3241 cccttgaatg ttggttgaac cttctggcgt aaagaaaaaa agttcataat cgtcttcaga
     3301 ttcgtacgta tcaatctcct tcaacaaaga agttttacct gtcagtttct tatggaaact
     3361 caatagtccc atagcaattt tacggttttc tttccggtaa tgtgttaaca tcttctcacc
     3421 ttcctatgac taagcccttg gaaaatgctg cttataaaca tccgtcattc ttttcttcgt
     3481 gatatgtgta taaatctgag tcgtagagat atccgcgtga cctaacaatt cctgtactgt
     3541 ccgcaaatca gctccatttt ctagcagatg tgtggcaaag ctatgccgca gcgtatgtgg
     3601 tgttacttct ttgtaaatcc cagcctctcg tactaactgc ttcagatttt tccagattcc
     3661 ttgccgagaa agacctttcc catgattatt gacaaataca tgcatttcag atgcgtcctt
     3721 tacaagtaat ggacgtgctt cgtctaagta gcgttccagc cattggatcg cataatcacc
     3781 taaaggaatg atccgctctt tatctccttt tccaactgtc tgtaccagtc caagagaaag
     3841 atgaagatct cccaatttca atccaatcaa ttcactgacg cgcatcccag tagcatacat
     3901 gacttccaag attgcacgat ctcttatccc taacgttttt gtcgtatcag gtgcttcaat
     3961 cagcttttct acttctgtca gagtcaatgt acttggtaat ttctgtgctt tcttcggcgt
     4021 atcgatatgt tgcatcggat catgatcagt gatacgctct tgccgtaaaa actgatggaa
     4081 tcttctgaga cttgagatca tccgagtaac agttgctgtc gctttttttt cttcatgcaa
     4141 cttctccaga aaacggatga ctaaaaagcg atcgacatcc tgccatgaag caattttctg
     4201 ctcctgtaga aaatgcaagt actgttccaa atcacgttca tagctttgtc gtgtattggc
     4261 ggacaatccc cgttcaatat tcaagtagtg gagataatcg ttgatttgct ctttcataat
     4321 aatccccctc atgcctctct atcatatcag ataccgcttc attctgatag tctttttcca
     4381 taatctgagc aaagaaaaag ccgtctagac actcagacgg actggtttct ttttt
//
LOCUS       DADXTG010000159         4397 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.162, whole genome shotgun sequence.
ACCESSION   DADXTG010000159 DADXTG010000000
VERSION     DADXTG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.162"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="IWI57_002480"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="IWI57_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBC2450579.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="IWI57_002481"
     CDS             complement(2017..2919)
                     /locus_tag="IWI57_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2450580.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="IWI57_002482"
     CDS             complement(2916..3608)
                     /locus_tag="IWI57_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBC2450581.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="IWI57_002483"
     CDS             complement(3651..4292)
                     /locus_tag="IWI57_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBC2450582.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1347 a          944 c          761 g         1345 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttac
//
LOCUS       DADXTG010000160         4377 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.163, whole genome shotgun sequence.
ACCESSION   DADXTG010000160 DADXTG010000000
VERSION     DADXTG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.163"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(83..1051)
                     /locus_tag="IWI57_002484"
     CDS             complement(83..1051)
                     /locus_tag="IWI57_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBC2450583.1"
                     /translation="MSKNGIIVNEWEKLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1501..2907)
                     /locus_tag="IWI57_002485"
     CDS             complement(1501..2907)
                     /locus_tag="IWI57_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBC2450584.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDIGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            complement(3129..3491)
                     /locus_tag="IWI57_002486"
     CDS             complement(3129..3491)
                     /locus_tag="IWI57_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450585.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3495..3833)
                     /locus_tag="IWI57_002487"
     CDS             complement(3495..3833)
                     /locus_tag="IWI57_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450586.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3812..4231)
                     /locus_tag="IWI57_002488"
     CDS             complement(3812..4231)
                     /locus_tag="IWI57_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450587.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4224..4364)
                     /locus_tag="IWI57_002489"
     CDS             complement(4224..4364)
                     /locus_tag="IWI57_002489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450588.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1235 a          989 c          618 g         1535 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aatttttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaacat aaaaccgcac cctttgaaaa gggcgccccc aaacttcgct aacgctaggt
     1381 tggccaaaaa atggactggc cattttttag caaatgtcag cttaaaaggc tagtggttta
     1441 gccgtcaact ccttatcctc ggcacaaacc gcctccggta ccgttgactg cacatcgtaa
     1501 ctaccactcg atctcatctt cagaacgctc taatttatgc tgtgagacgt tttgacgata
     1561 aaataaggtt ttctcctgct ctaatacttc taattcctca gaaagcccta tatccttttc
     1621 tgcaagacca aaacggatag acaaacgagt gatcgatttt tgaaatgcgt tttttaatcg
     1681 ctctaaacgc tggcgtaaag agcgattttc ttctttcagt tttttatttt ccacttgtaa
     1741 atctgctaat tgttttctac cagacaaatg acgctcttct tccaatgtag ctacatactc
     1801 atttaattct gtattttgct caagcatctg taaatattgc tcactcatgt tatcagcatt
     1861 tctcttggca tttgttgctc tcgtagctcg ttgatacaaa tcaacaaaat cattcccaga
     1921 taaatgatac gaaccatcag gaagcctctt gaaactttct aaaggaacca tatctttttg
     1981 gaaaatgacc gccatctctt tttttacatt cagcatattt ttcttctctt cttcaatctt
     2041 cgctctctca gaagccatca gagccttatc acgctcgttt tcttctcttt gcctataaat
     2101 atacctcaac tcatcagaaa cgctctcacg ctcttttaca gccgtttggc gcaattcaga
     2161 aatttcactc atgatttctt tgtattcgtg aatatcttta attttgttgg taccgacaat
     2221 atcccttttc caaccgtaat cattcattaa tcgttctata gagtcaactt cttgatttct
     2281 aaaattattg aaaagcactc gaccattatc tttatcagcg tgtatccctt gttgaaccag
     2341 tgctttatta aagctcggct gcttttgaac accccgttta taaccttcag caaccggcac
     2401 aatattcaag tgcaggtgcg gacttatttc gtcattatga ataacagcat tataaacagc
     2461 caaattaggg tttctctctt gaaactcctt gtaatactga accaaaattt tattggcaat
     2521 cactcgattt tgttcagctt gttgtttcga tatgccagta gaagaaccat ctgctcgatt
     2581 tgtctggaaa tcatctaaaa tgcctatttg cacaataaat tctcgttgag gttccaactt
     2641 tttatccttg cgcactttcg ctaaatagtc ctcgattttt ctatcggctc ttttttgttt
     2701 cgcattatat ttttctacca cttcgccaaa caattcctca tatttatctg ctagattcgt
     2761 ctgctctaaa taaatatttt catccgattt cgtgaagtca atatgcttat gccaatcatt
     2821 ggttttctgg ctatcagtta actcacgatt attatgagaa atacttgtat tcttcgttcc
     2881 ttttttaaat gaaatactaa accccaagtt aaaaccctcc ttcgcgctgt cgctaataaa
     2941 aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta attgtgacat
     3001 ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga caacgaaaaa
     3061 aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc ttcacgcaaa
     3121 ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact ctcactcatc
     3181 tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg ataatctcgt
     3241 tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt ttcgtgttgc
     3301 tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc aaacccaaaa
     3361 cgcttgcgaa gagccttgcc cattgcagca ttttctttct caatcaagcg ttttttctgt
     3421 tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat atctgctaaa
     3481 gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat ttggtttaac
     3541 tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc agttgtttgc
     3601 gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctag atcagcattg
     3661 ataaccgagt tgagaacatc catcattcgt tcaagcattt cactataatt atcttcatcc
     3721 ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc gactgtttct
     3781 tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac cattttcgat
     3841 tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga agcaaagtca
     3901 atttttgttg gtcacgaatc atttccgtta attcagaaat atgcctatct ttttccgcta
     3961 attgttcacg caaaaattca ttttcttttt ctaacgggaa atcattgcct gcatcattga
     4021 ccggtaaatc accagtcaat tgaccacctt ttgaccgata aattgaccgc acaacaccaa
     4081 tcaattcctt gtctaacatg ataaccccat tatctttata ccgagcaagg tcataacctg
     4141 cattgaccag ttgattgcgc actgtatttt tagatatacc caactcatca gcaaggtttt
     4201 ttatggtcat tttttctggc aaatcagtca ttatcttctc cgttactttc agctacccca
     4261 taaacatatt taacagcatg cttagggata atgattgggg aatcaatttt attatttttc
     4321 aaaatcaact catactgcgt tacatcaagt aacttaccgg acattttttt gctatcc
//
LOCUS       DADXTG010000161         4345 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.164, whole genome shotgun sequence.
ACCESSION   DADXTG010000161 DADXTG010000000
VERSION     DADXTG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.164"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            154..1002
                     /locus_tag="IWI57_002490"
     CDS             154..1002
                     /locus_tag="IWI57_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBC2450589.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1147..2088
                     /locus_tag="IWI57_002491"
     CDS             1147..2088
                     /locus_tag="IWI57_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBC2450590.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2078..4249
                     /locus_tag="IWI57_002492"
                     /pseudo
     CDS             2078..4249
                     /locus_tag="IWI57_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1355 a          855 c          810 g         1325 t
ORIGIN      
        1 gtattccctt ccattacaat gaaataaata gcgattttat gagaaggtga tgtgaaaatg
       61 gaagaaaaaa tagaagaatc caaacaaaaa atcgatcagt tgatataaga attagaaaaa
      121 gcggtcatcg gcaaacgttc aactatccaa ttgatgatcg tggccctatt agcaggtggt
      181 catgtcttat tcgaagatat tcccggtgta ggtaaaacgt tactggtcaa agcactagca
      241 aaagctgtac agggaacctt cagcagagtc caatgtactc ctgatctgct gcctagtgat
      301 attcttggtt tttccgtata caattcacaa tcaaaagagt ttgaatttcg tccgggaccg
      361 atctttacca cgatcttgtt agcagatgag atcaatcgta cgactcctcg tactcagtca
      421 gcacttttag aagcaatggc cgagaatcat gcaacgatcg acaacaatac ataccctcta
      481 gacaatcatt tttttgtctt agctacgcaa aatccaatcg agtacgaagg aacatatcct
      541 ttgccggaag cacagctcga tcgtttttta ttccgactac aaatcggtta tccttctttt
      601 gatgacgaac ttttgctact tttggataaa ttcgaaaaag tgttggataa cttaaatgaa
      661 gtattgaaca gttatgaaat agaagaaata aaaacaaatg tggcgaatgt ctttgtcagt
      721 cccgaagtgg catcttatgc cttgcagtta gtgtcagcaa caagaagtca tgctgccatc
      781 cagcttggca tcagtccacg aggaagcctt tcctttattc aggcagcaaa agcttatgct
      841 ttaatccatg ggagaaatta cgtcacgccc aaagatttgc aggatttagt tccttacgtg
      901 ttttcccatc gcctgatttt ttacgatcgt tcattgaagg aagacgaaaa gaaacaaatc
      961 attcatactc ttgttgcaca aattcctatt cctgtgaggt agattgaggt agacaatgga
     1021 aaaaatcaaa gattatttca ttaattttgt gttatttctg gtatatttca tcattgtgct
     1081 ttttgctctt gtattcaaca attctttagg ctggttcttg ttggcctttt ttactcttta
     1141 tgtatcatgg agcttgattt cattaagcag ctttctaaag aaactttcgg ttacttgtac
     1201 attgcagcca ctcgtttatc gcaaaaaaag tatgcaggtt attttttcca tagcaaaaaa
     1261 gagaactttt tctttacctc tccctcgttt acaaatcggc ttcccagcaa tctttgcaga
     1321 aaaaaacaag agattttgat tcttacaaat aaaccgcaag aagtccaatt gctttggcag
     1381 ccaaaagaaa gaggatgcca tgaaacatta actgtcattt ttacagccta tgatagtcta
     1441 ggacttttca gaaaaagtgc aaaaagagag atctcttttc ctgtgactgt tctgcctgct
     1501 ttttgtaaga ttcaagcaga aaaattacag agagttctgg aaaaaaaaca gcagctgaac
     1561 gcgtaccaaa gaggtcttga ttttcgggaa catcgcgctt atcgtccagg tgattctttc
     1621 aaccgaatcg attggaaatt gtctgctcat actcaagagt ggttataccg tgaatacgat
     1681 tatcaagaag aagaatgttc tcctcttttt tgcttttggg gttcgcccag tccaaaattt
     1741 gaacctctgc tagacttgta tttttcttta tggcatttga gaaaagaaaa gcagccggaa
     1801 cttctgatat taggcaaccg cgcctttcat ggcaaagacc ccggtcatac ctattttgct
     1861 tctttggaag ctggcgatga gaaaactttt ttcgcacact tgaaaaaata tgcaaaaaaa
     1921 caaatcgtcc tctttgttcc tgagaattct cctgaagtct cagaagctat tgaaacattg
     1981 tcaaaagatc gaacggttta tcttgtttat ttcgtagaaa aacaactcat ggtccaagaa
     2041 aaagacaaga tatgttcatt gccaggaggt gaatggattg acgattaaac gggaaaaatg
     2101 gctgcgtggc gtttactcct ttgtgatact aagtgtaggt gctccgatat ttgtagtagc
     2161 taatcaattg caaaacagct accttcttat cctaactatt ggtgtcctat gccttattgt
     2221 ctgctgtatt cctaaaaaat gggtacgttt gctgctttct ttgccagtaa tgataggttg
     2281 tttgttttta tattttcctt cgaagacatt ttccatatta tggatcttac agtttttttc
     2341 gtcagttaca gaggaattct tccatctatc ccaaggtaaa ctttattatt tgccagaaaa
     2401 gacagctttt tttctcataa tgttgctgat ctatttattt attacattga ccgtggatta
     2461 cgactattgg tacgcaccct ttctttcttt gatggcttac ttcatgatgc ttaccgtttt
     2521 tcggaaaaaa gatttactgt acgaaatgat tgcggtagta actgttttgt tcgtgtatct
     2581 ggcagcaagg caatttttct ccatccggtt gttgtcgaga aacactcgat tccagtcatt
     2641 gactacagtt ctgcttattt gtttcttaac ttttagcgct atcttgcctt tatatcttcc
     2701 gaaagtacac caatctctcg aggaaacgag tgaaccgatc cgagaatatc ttaatgatga
     2761 agggctttat gacacactcc atgagtatca attaaccgga agtgcaagaa caggcttcag
     2821 tgagaatgat gaacagcttg gtggaccttt atatgataat ggcgaagttg tttttacagc
     2881 gaaccaaaaa ggaaatggct attggaagat cgaaaataaa aatcattaca ccggttctgg
     2941 atgaatagaa gatcaggacg cacccatcca accaatccaa gaattccctg ttgacatcac
     3001 gagctaccaa accgttatag aagacaaaga aaacattaca ttgaccctaa aaaaacaagc
     3061 tttcttacct tatccttatg gaaatactac cttgactgat ttttcattag ctagtggaca
     3121 aacgctttac tatttgcctg aaagccagcg attcattcct gtcccgaatg aatcgacacg
     3181 ttctgcaact ttacaaacag aaaggttagt ttttacgaga gaagaattaa aaagcagtca
     3241 gttaaaagct gatgatttag aaaatcagca gacaaatctt caattgccaa aaagtttgcc
     3301 taaacgtatt gaggacttgg cggtcgaatt gaccaaaaat gaagcaactt taattcgtaa
     3361 agtcgaagct gtctaacagt atttaaaaac aagcggaaat ttccgctatt ccaaaacgga
     3421 taccgggcat actccaaaag aacacgatta tgtggattat ttcctctttg attcaaaagt
     3481 cggctattgt gacaactttt caacttctat ggtcgtcctt cttcgaacac ttggtatccc
     3541 agctcgctgg acaaaaggtt ttacaaatgg gctcagaaca ggcacagata atcaagggaa
     3601 tgaggaatac agcattctca atagtcatgc tcattcttgg ccagaggtat actttcctgg
     3661 atttggctgg cttccatttg aaccaacccc ttcatttgcc aatccaatgg gttcacaacc
     3721 agttgctgaa acaaccgaaa ccgtccagcc tgctatcgaa caaccaaatg aaagcgaaaa
     3781 tatggcagaa actatttcca gtgagagatc tgaggtcatt ccatcagaag aaacaacact
     3841 cacaccagct tataaaatcg tcttattcag ccttggagtg tgtttatccg tcttgttgtt
     3901 ctttctattg ttattcagaa aatctctgcg ctggacaagt tatcttatct atcttcattt
     3961 ttttgcttct actaatacga ttgcatatca aaaacttctt aaccctttgg aaacattggt
     4021 accgcgcaaa cccgcagaat ctttgcagga atatagcctg cgaatagaac aagaattcaa
     4081 gctagatctg cgtttcattc gattaactac tcaatatgaa gcagaaatat atggacgaca
     4141 agcagatccg gtaaaattcg ataaaaaaac ctatttagaa ataacgcata taataagaaa
     4201 aacggtatac aaaaaaattc tgacaaaagg gagccgcttc aaaaaataag ataaaatttc
     4261 tggagaatct acttctcgaa tgccgagaac cagctgattg agtatcgatt actcgaaaaa
     4321 aaggttgtga cacgtttttt gtcac
//
LOCUS       DADXTG010000162         4174 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.165, whole genome shotgun sequence.
ACCESSION   DADXTG010000162 DADXTG010000000
VERSION     DADXTG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.165"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..1347
                     /locus_tag="IWI57_002493"
     CDS             46..1347
                     /locus_tag="IWI57_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBC2450591.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1374..1634
                     /locus_tag="IWI57_002494"
     CDS             1374..1634
                     /locus_tag="IWI57_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBC2450592.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2633..3868)
                     /locus_tag="IWI57_002495"
     CDS             complement(2633..3868)
                     /locus_tag="IWI57_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBC2450593.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1204 a          670 c          868 g         1432 t
ORIGIN      
        1 tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga aaagctgatg
       61 aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca gcgtcattta
      121 ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg gacatttttt
      181 atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc ttattcggca
      241 acgattgttt taccctttag gatgacgatg ggattgatgg ctatttatgc aacttttgtt
      301 atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg tatattatct
      361 ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga agaggcacct
      421 cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc ggggatctta
      481 tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat tactatcaaa
      541 atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat tccaggtgca
      601 gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt aaacgctgcc
      661 ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct tggtgtttta
      721 ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat gagtattgta
      781 ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa ggcattggca
      841 gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta tcaatggacg
      901 gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat attcttctgt
      961 aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat cttcaatatc
     1021 aatgagccca taatttttgg agctcctatg atgttgaatc cttacatgtt catcccgttc
     1081 aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt agaaatggtc
     1141 aatagattta ctgttttgcc aacatggaca ttaccagctc caatcggcgg ttatctttca
     1201 gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat agcatttatt
     1261 atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga acaaagtcgt
     1321 ggagaaaacg aagtccagac tgtctaagtt tggtttttag aaggtgggaa acaatgtatt
     1381 ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt atattgaata
     1441 tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca tcatttttga
     1501 tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca tgggggacat
     1561 atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga tggtttgtca
     1621 catcgaagct ttaaatgttc taaaagtggt tgtaacagaa gtggttagct tcaagaaata
     1681 agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt atttttcgtt
     1741 tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg aatatgggac
     1801 tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt tttaactgtt
     1861 ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc aatggattta gaattagtag
     1921 atctactacc aaagtgatca agtcaaatat agatttggga gcttacaacc atatagtagg
     1981 ggctgcagta tggtgtatga atggtaatat gtatgatgtg agtgtttatt caaatcatga
     2041 aacgtgtata gaaataattg tgctgggaat agcaatcagt catggtgaac gtgactttta
     2101 gtgtatcgtt gctgacgaca tagaacattc tgacatgatc tactcaatat gtggggattg
     2161 ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa cagaagaggg
     2221 aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat tgttgagggg
     2281 actagcggca caaatctcta caaagcgatg cttctagatc tatatgacac attttaagaa
     2341 aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat gatccaacca
     2401 gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta tgctcatttg
     2461 gatagcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac cgtaatttaa
     2521 gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt ctgggtctcg
     2581 aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga aactatattg
     2641 ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca tttattggaa
     2701 aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact tcgcgcagag
     2761 caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt ccgtatttta
     2821 cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata aacggtttgc
     2881 cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa aaatcaatga
     2941 attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct gagtcagatt
     3001 gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt tgtttttgta
     3061 agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt gcgaaataga
     3121 taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct tcaggcaagg
     3181 aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc agctgttctt
     3241 cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc atttgtaaac
     3301 ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca ttggggatcc
     3361 atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt aaagggccga
     3421 agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt tcactcaaag
     3481 catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa aaaattgtca
     3541 cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc tgttcactag
     3601 tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc aactggtcaa
     3661 attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa tgatactcaa
     3721 agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat acacagtaat
     3781 aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct aaattgaata
     3841 ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga gtctatccct
     3901 tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta tattttgaat
     3961 attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa tcaagttgaa
     4021 actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatgt tgtgccagat
     4081 aaatacgaga agttgtgtca ttaaaaataa acatgagaac ataaaaaaat aaaataatta
     4141 tattaattga taggtgtgct gtcgtcatta taca
//
LOCUS       DADXTG010000163         4146 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.166, whole genome shotgun sequence.
ACCESSION   DADXTG010000163 DADXTG010000000
VERSION     DADXTG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.166"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..715
                     /locus_tag="IWI57_002496"
     CDS             14..715
                     /locus_tag="IWI57_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBC2450594.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1287..2303)
                     /gene="tsaD"
                     /locus_tag="IWI57_002497"
     CDS             complement(1287..2303)
                     /gene="tsaD"
                     /locus_tag="IWI57_002497"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBC2450595.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2300..2764)
                     /gene="rimI"
                     /locus_tag="IWI57_002498"
     CDS             complement(2300..2764)
                     /gene="rimI"
                     /locus_tag="IWI57_002498"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBC2450596.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2771..3313)
                     /gene="rimI"
                     /locus_tag="IWI57_002499"
     CDS             complement(2771..3313)
                     /gene="rimI"
                     /locus_tag="IWI57_002499"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBC2450597.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3297..4019)
                     /gene="tsaB"
                     /locus_tag="IWI57_002500"
     CDS             complement(3297..4019)
                     /gene="tsaB"
                     /locus_tag="IWI57_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBC2450598.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1216 a          887 c          722 g         1321 t
ORIGIN      
        1 agaggacttt ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca
       61 acctgatttt gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct
      121 tcgttcttat aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta
      181 tatcttgacc aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta
      241 ctatgactat gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac
      301 ctacacttat acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta
      361 ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc
      421 taaagtagtt gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga
      481 ccgggtaaaa caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg
      541 tcatatcacc attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac
      601 acgtgaaact gctggacaag taatcaaaca actacgtgat tccggactgt tagattatga
      661 caaaaagcgt tttactttct gtaagacagc tactactcca gctatgagag aataacgcga
      721 acaaaaataa gcagtgaccc tgatatttat cagggtcact gcttattttc ctccctaaaa
      781 cttctgaaag atccggcagt tttagatcac tgggcaagac tcaaagattt tctatgtgtg
      841 tttcagttat gtaatattca ctgtttgcaa aaaatactga tagattaact agaggaagcg
      901 tcttgtcctc atccgaccta gattattcga tattcctcga caataaacga ttaatcagtg
      961 agggcaatca gaggaacgga taatgatgaa taagctgtaa aaaatagggc tttgacaaaa
     1021 gcgtgtcaca gcccttattt cgattaaacg gtgtgtacct gtcaactctt cggtcacaag
     1081 tcataatgtt cataaacatg aggagctgtt ttttgaaatt gttccttgtg aatcagagtt
     1141 aaacgacagg cttacgaact cttgattaaa cggcgttcaa aagcttgcat acgcggtaat
     1201 aagaacagcc aggtcaaaaa catgagaagc cgtttttcct gggctgttct tattacttga
     1261 tgcagggcag atttttcaca acctctttat tcagataaaa ccaagcttgg atcagcattc
     1321 aactgatagc tagcaaaatt tcttttttgc atttccacat gtgctgctgc tccaatcatt
     1381 gcagcattat ctccacaaag tcgaagaggg ggaatcacta gttctacctc agggagttta
     1441 gttgccaaag catcctgcaa tccttcacgc aacccttgat tggccgctac acctccagct
     1501 acaatcagtt gcttgactgg ataattttga caagctcgaa gtgttttagt aatcaataca
     1561 tcgatcacac ttgcttgaaa gctagctgct aaatcatttt tgtctagatc ttctccacgc
     1621 tgttgtgcat tatgcaccaa attgataaat gcacttttta atccgctaaa actgaaatca
     1681 taattatctt catggatcat cgctcgtgga aaatggtaat tatcttttcc ctgatgagct
     1741 agctgatcga tttcttttcc gcttgggtaa gaaagaccta atactcttcc aactttgtca
     1801 taagcttctc ctgctgcgtc atctcttgtc tccccaatga tttcatatga tccatcttct
     1861 tgcatataga ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg
     1921 aatggtttaa ccagacgggc tgcatagata tgccctgcca tatggtttac aggaataagc
     1981 ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa taccaatcag caacgagccg
     2041 acaagtcctg gtccataagt gacagcgact gccgataaat cttctgccga cacaccagct
     2101 tctaccagtg catcttccaa gcataaggtg atttgttcta cgtgatggcg gctagctact
     2161 tcaggaacta cgccaccaaa acgtttatga ctgtttattt gagaagcaac gatgttagat
     2221 aagatctctg ttccatttct aacgaccgct acactcgttt catcacaact tgattcaatc
     2281 gccagaatca attcttcatt catttcatcg tctccgtttt taactttctg ctcataatga
     2341 tcgcatcttc cgtcggattg cgataatatg atttccgttt acctaatata cgaaactttt
     2401 ctgattcata aagttttctt gctgcttcat tagatagacg tacttctaaa aaaacatgca
     2461 gcgaatcttt tatttgttcc gtaatcagta attgtaacaa atgccgtgca tgcccttttc
     2521 cttgttctga aactgcaaca gcaatatttg tgatctcgac ttcatctatt actttcgagt
     2581 atccaataaa gccacagatc tggttatctt caactaaaag cagataatca gagtgaggct
     2641 gttggatgtc tgccaaaaac tgcattttcg tccaaggtga accatgtgga taagcatttt
     2701 cgctaatcat ccatacgcat tctgccaagt acgcttcatc aaaatctttt tttctgcaaa
     2761 tcaactaatc tcactcattt tctaaaagtt ttatcatatc caacgcatct tcttttgttt
     2821 cagtgtaata tctttttttg atcgttgttg cttgaaaacc taacttccga taaagtcttt
     2881 gtgcatcttg attactcata cgcacttcta atgacatcgt ctcacagcga ttcattatag
     2941 caaagttctc gatttcgtca ataagcagcg aaccgattcg tttacgctga tatgccggtc
     3001 gaacggcaat attcgtgata tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa
     3061 tcattcttcc attatcaaag atacctagat atagatgagg aagcggtgac ttcaattctg
     3121 atataaaggc actttttgtc catggcaatt cgccattata gacttctcgt tcgacatcca
     3181 gcagctcttt gatatcttct gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt
     3241 cgattgtttt ttctgtataa atcattttat cttgaaaaaa gcttctggca tacgctctaa
     3301 attttttcaa cataactttc gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct
     3361 tctacccgtt ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg ttcagctaaa
     3421 gcacctaatt gagccacagt tacaccactt ggaatatccc atgctggaac tgtattaatc
     3481 atcgcactag gcaatatttc ctttatttca tagaagaatt tgtgacaatc actcccgacg
     3541 aaataaatag cttcattcag cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt
     3601 acatcttcaa gtacaatttc taatctattt tctttccagc ggtatgcccc agcgtataca
     3661 ttttttcttc gagcatcaaa catgggcaca ataattttat ccacaccgat gcaattagcc
     3721 gcgatggttt tcagactaga tactcctact agctctttat ctaatgtata agcaatcgtt
     3781 tttgctgtcg ttacacctat acgaagccct gtataagaac cgggaccatc agaaaccgcg
     3841 attcggtcaa tgtcttttgg acttatttga agatctgcaa acaattggtc gattgcaggc
     3901 atcagtgtta cactgtggtt gcgtttgata tttgtttgta tttgtccaag aatttgttcg
     3961 tcttctacca caccgatcgc caaagtttgg tttgcagtat ctattcctaa tgtaatcata
     4021 tatttgatcc tttctgctat gacattctcg ccttccattg tagcatactt ttttattctg
     4081 aggatgaaaa taccaaaggc aaaactagta caatgataca tgattatctg ctggcgggca
     4141 aatttt
//
LOCUS       DADXTG010000164         4061 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.167, whole genome shotgun sequence.
ACCESSION   DADXTG010000164 DADXTG010000000
VERSION     DADXTG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.167"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..362
                     /locus_tag="IWI57_002501"
     CDS             <1..362
                     /locus_tag="IWI57_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2450599.1"
                     /translation="NIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDF
                     IKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANL
                     KTSSSFEKLVYILKEGL"
     gene            363..809
                     /locus_tag="IWI57_002502"
     CDS             363..809
                     /locus_tag="IWI57_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBC2450600.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            825..1139
                     /locus_tag="IWI57_002503"
     CDS             825..1139
                     /locus_tag="IWI57_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBC2450601.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            1165..2214
                     /locus_tag="IWI57_002504"
     CDS             1165..2214
                     /locus_tag="IWI57_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBC2450602.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            2226..>4061
                     /locus_tag="IWI57_002505"
     CDS             2226..>4061
                     /locus_tag="IWI57_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBC2450603.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLILIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLA"
BASE COUNT         1432 a          583 c          847 g         1199 t
ORIGIN      
        1 taaatataat taccaaaaaa ttggtaaaag aaaattacgc gaaagaaggg atactagagg
       61 gagcaatcaa aagagaaaaa ctatcttcaa cagcgatgaa tgattttgct atgccgcata
      121 tcgaagtaga ttttataaaa aagccagtaa tctgtgtcta cgttaatcca gaaggaattg
      181 attgggatgg gacacttgtt cattctgttt tcttttttgc acttaatgaa aaagtaaaac
      241 ctgaattgaa taaaatttat gaaacattca atgaaatcgc ttcgaatcat gagttattag
      301 caaatttaaa aacgagttcg tcgtttgaaa aattagttta tatattgaaa gaaggcttat
      361 aaatgatcag tgaaaaaatc aatataaata atatagtgat agatttaaat gcatctgata
      421 aaaaaagtgt agtcgaaaaa ttatcgaaat tgttagagac taatgggttt gtgtctgacc
      481 acaaacaatt tcaaaaagac gtatttgaca gagaaaacca tatgaccaca ggaattggga
      541 aaaatatcgc cattcctcat ggcaaaagcc tagcagtaat tgaatcaaca gtggctgtag
      601 ccaagttaaa gcggtctgtt gaatggggcg cattagatga tcaaccagtt gatctcgtct
      661 ttttgttagc aataaaagac actgataaag gtactaatca tttacgtatt ttagcagaat
      721 tatctggaag attgatggat gacgagtttg ttaaaagaat aaaagattcg agaaataaaa
      781 aagaattggt aaaagctcta tcattttaaa aggaggaatt tcatatgaca aaaatagtag
      841 cagtaacagc atgtatcgca gggattgccc atacttatat ggcaaaagct aatttagaaa
      901 agtttgcgaa gaagaaaggg ttagatatta aagtagaaac acagggggct atggggatag
      961 aaaacaaact ttcaatttcg gatgtttccg aggcagatgt tgtcatattt gcagtagata
     1021 caaatgtttc agaacgaggt cgctttgacg gtaaaccgat tttaacagta gggacctcag
     1081 aggtagtaaa agatggtgaa gagacgattg atgaagcaat tgcattaatt aacaaataaa
     1141 aaatatatcg tgggggagtt actaatggaa ttcaagaaac aaggaagaga aattctaaat
     1201 catttccaaa gtggggtttc ctatatgatc ccgcttgtcg tagcagcagg gttactaact
     1261 tctattgcag tcatttttgg tggcacaggt gtttgggacc agacggatac attctgggga
     1321 gtcctgcgaa tgattggtca gacgggatta cagtttatcg taccgatgat ttcagcctat
     1381 atcgcatatt caattgctga ccgtcccggt ctagcaccag cttttatcac tgggatgatg
     1441 tgtcaaaatc ttggtatggg atttattgga ggaatggttg ctggattagc ttgtggctat
     1501 ttagctaatg cattgaaaaa agtaaaagta ccgatgcaag ttattagtct aaaatcaatt
     1561 atgctgattc cttttgttac tacattagtt gttggaatca tattatggta tgtactagct
     1621 acgcctattc aagcaatgac aacaggactg actaattggc tagaaggcat gtcaggagca
     1681 aatgctgctt tgatgtcggc tattttagga ggaatgatgg cgtttgatat gggaggacct
     1741 atcaataaaa tcgcatatgc ttttggggtt gcttcttttt caagcggaag ctttggacct
     1801 agtacagcca tgttattagg aattgctatt ccgccatttg gaatgttttt agcaacaatt
     1861 ttaaacaaaa aactttatga tgaatcggaa cgagataatg caaagacagc tatgattatg
     1921 ggccttgtgg ggattactga agggacgatc ccgtttgccg tgaaagatcc tttacgagta
     1981 ataccaagca tcgtggtggg cactgcagta gcatgtggtc taaatggatt ttttggtgtt
     2041 actcaagaaa caatgctttc aacctttatg gcaattcctt ttgttacgaa tataccttta
     2101 tatttagtat caattgttat tgggggagtg gttacagcgt taatggtcaa tgcactaaaa
     2161 tccatcaagt ataaaaaaga acaacaagta aattcagtag aagaaggaaa gtaggagaat
     2221 ctgatatggt aaatgcacat ataattcaac acacgcactg ggacagagaa tggtatttta
     2281 caaatgaaga tgccatcgta ttgtctgatc aggtctttac tgaagttcta gatgaattgg
     2341 aaaggaaccc taaagtaaac ttttgtttag atggacaaac ttcaatcgtc gatgagtatt
     2401 tggagatcaa tccgcaaaag cttaaggtta tccaacgact gatgaaaaca ggacaactat
     2461 tcgtgggacc gtggtatgca caaacagatg ccttattagt ggatgctgaa tcaattttaa
     2521 gaaacttaat gattggaatc agtgatacga tccttaaata tggagatcca atgatggtag
     2581 ggtatttgcc tgacacgttt gggttcaatg cgaatttgcc tacacttttg catcaagtag
     2641 aaatcgataa tttcatgtgt tggcgaggat taaactttga aacgatggtt ccatctcctt
     2701 attttatctg gaaaggattg ggagataaat ttgtttatgc catgaatttt ccatttggtt
     2761 atatgactgg aatgatgacc ttagacgcaa ttaaagatat tcctgaattt gcaaaagaaa
     2821 agttggatga agcagttgaa atgcttcata tgcaaggaaa aaacaaagat attttagtac
     2881 cttcgggtgt agatcaaaag tcaatgattt taaattttga tcaagtcgta gagaagttga
     2941 ataaatacag taaattccac aatattattt cagattatcc ttcttatgta gaagttatca
     3001 gaaacaaaaa agaattacct gaatataaag gtgaacttcg tcagccggtt tactctcgtg
     3061 ttcatcgttc tattggctca gtaagaacaa aaatgaaaat agaaaatttt aaattggaac
     3121 aaaaaattct aaaacgagtt gagccgctca tactgattgc agaaagaaat ggtatccata
     3181 tcagtaaagg cctacttcgt cggttatgga aaaagatttt gcaaaaccag gctcatgata
     3241 gtattggtgg ctgtgtttca gataatgttg cggaagatat tttccatcga atcaaagaag
     3301 ctaatgagat agctgacgga atcgaaaacc ttattgtaaa acggatggct gatgcattaa
     3361 agctaaaatc gaatgaagtc cttgttttca acacaacgac tgagaattat agtggatcga
     3421 aaatcgtgca tgtagtagct gattcaaaga aaatacactt caaaggtagt ccctataata
     3481 tcattgaaaa agaagtttat tttccagaac ggaaaaatgc ttatcggatg attgctacag
     3541 gtttcgaata ttttgatgaa ccagcttatt atgaacttga cataaaaata aatgtaaatg
     3601 tgccagcact aggatacact gttgttgaat ttgagaaaaa taatgaaaaa ttagaaacag
     3661 caatcgagat agacaaaact gagataagta acaataaata taaactttct tttaaagatg
     3721 ggcaattgaa tttgatagtt ggagatagac aatatcttga tttcgttcat ttgatcgatt
     3781 cagcaaatga tggcgatacg tatgattatt caccattaga aggagataca gaactatctc
     3841 taaaattaga aacagctaaa gtatataaag attctttaca agaaacatta gttgtttacg
     3901 gaaaagctca acttcctaaa gatttaaaag atcgtttatc tgaaaaaccc gaaatggaag
     3961 aaatatctta tgaaatatcg ttcagtctgg gagaaagtca aatcgttgaa ggcacactca
     4021 aaattcataa tactgtttac tcgcatcgta tgcgcttagc a
//
LOCUS       DADXTG010000165         4054 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.168, whole genome shotgun sequence.
ACCESSION   DADXTG010000165 DADXTG010000000
VERSION     DADXTG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.168"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(46..1812)
                     /locus_tag="IWI57_002506"
     CDS             complement(46..1812)
                     /locus_tag="IWI57_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450604.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1772..3526)
                     /locus_tag="IWI57_002507"
     CDS             complement(1772..3526)
                     /locus_tag="IWI57_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450605.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3636..>4054)
                     /locus_tag="IWI57_002508"
     CDS             complement(3636..>4054)
                     /locus_tag="IWI57_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBC2450606.1"
                     /translation="YISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILA
                     DIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAM
                     IHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1358 a          771 c          572 g         1353 t
ORIGIN      
        1 ataactatat cccaaaaatt gtaggttaat ttaaagccta tttcatcatc gtgattgagc
       61 ttctttttca tagacgaatt tctcagatag tttagttttc tttatgagat ttttgtatat
      121 ttctgacctt cgatacaact cattatgagt accttgatca actagttttc cttgatccat
      181 cactacaatt ttatttacct tttcaataga cttcaagcga tgcgatataa ttagtactgt
      241 cttatcttgt attaaacgat ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa
      301 actggcagta atttcatcta aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat
      361 ggataggcgt tgtcgctctc ctccagaaag tgaacttccg ttttctccga ccaatgtatg
      421 ataaccgtca ggtagttttt caataaagtc ttcgcagtta gctaaacgag ctgctttttt
      481 cacttcctca tctgacgctt gactatttcc aatacgtata ttttccaaaa tacttgtatt
      541 aaacaaagtt acctcttgaa aaacaatagc gattttgcta aacaaagaag ctggagatac
      601 acgtttaata tcatacccat caattaaaat acagccttca tcataatcat acagtctaga
      661 cactaatttc aagatactag ttttcccgga accactagca cctactaacg cagtcacttc
      721 tccttgttta gccgtaaaag agatatgatc taaaattggg gtattgttat catatgagaa
      781 agagacatct cgcaactcaa tgtcaaaaga tttcagagga ctatctgatc cctcttggat
      841 actggtttcc ttcatagctc taattcgctg tattttagga gctaaataaa atatttcaag
      901 cactgcttct ttcatagaat cgaaggaatc ctttattttt attgcggcta gtaagtaacc
      961 aacgacgtag agtatagtca cttcacccgt taacagtaag tgaacaccta ctagaatgac
     1021 tactgctaaa ctaacatatg agaaaataga ggataaggcc atgactgaaa aagtacttaa
     1081 ttctaccttt aaatgaatcc tttcactctc ttccattttt ttgaaaagtc tgtcttgaac
     1141 ttttttagat agattaaagc tattaatctc ttgctgcaat tctatagttt cttgaaattc
     1201 ttccgagttt tctctcaaag tatcgtaata tttagtattg gctttcgttt gggatttctt
     1261 tgataacaag attaagacaa aactaaataa cgttggcaaa ataacagcta atcccatttt
     1321 gacattacca accaaaagca tcactgaaat aaaagggaaa aacagagcca taccgccggc
     1381 tttaggtatt gcatgactca tcgcatgctc aataccttca acgtcagaca tgatagtttg
     1441 agataaatct gataaattat gttttgaaaa ataggataat ggtaaatttg acaaatcgtc
     1501 tgcaatttgc actcttaaat gcgcactttc tttataagta ctattatata agttttcata
     1561 ctccctatct aacaaccaat acatgaaaag caaagtaaca aacgatacgg caaaataaag
     1621 ccaacggggc tttgtatttt ctaaaactaa ttcgtctatc agcatcatta taatgaatgc
     1681 tggtaccata ttaatgcagt atacaaaaaa actagaaata gaagctttag ttaaagcttc
     1741 tgcgccctga tcacttaaag caaatttgtt tttataaaaa cttttcattg tcaagcctcc
     1801 aatcatttgc attctcataa ttagtgacca attgtttata cacttgatgt ttagccatta
     1861 attcattatg atttccacgt tctactattt tcccttctga caagacaaga atttcatcta
     1921 cagatttaat gctggataat cgatgagcta tcataataac tgttttttct ttcatcagat
     1981 gttgaaaagc cttttgtagt tcatactcat tatctggatc aatagatgca ctggcttcat
     2041 ccataataac aatctttgca tcttttaaaa ttgctctggc aatagctata cgttgttttt
     2101 ctcctcctga cagataaact cctttactac caattaaggt atgctctctt tcgggaaatt
     2161 tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc aaatacttta cttgctggcg
     2221 cttgaggatt tgctatagaa acgttatcgt aaatactctg attgaatagt ttcgtatctt
     2281 gaaagacaaa agcaattgtg ttaataattg tgtcttttga atagtgattt aaaggcttac
     2341 caccaatttt aatctgtccc ttatccacat tgtagaaacc tgaaattagt ttagctatcg
     2401 tactctttcc gcttcctgat tttcctacta atgcataaat tcttttttgt tctaaaacta
     2461 agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa
     2521 tgtcacaatt tttaatctgt gtctcgttcc catattttag ttggtcttgc ttcatttcta
     2581 aataaaggga gtctagtgtt tcgactgcat atcttgcttc aaacaagtgc atacttacat
     2641 acattattct catgaaagaa acaaaaataa ttccacttaa aaaaaacaac atcaacaagt
     2701 caacagctaa tatcttggga gtggtaagag aagttataaa aaaggttata ggaactatca
     2761 aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac aaaaggtgtt ttcccactca
     2821 aagaatactt atatgcaaac ttagcataat cgttaattgc tttattcaag gctttaaatg
     2881 atgttacttt agcaccaaat atctttataa cttgaattcc gcggatatat tctaccgttt
     2941 cagagcttaa cacatctaac gattcttgat atttttgaat aaaatttgta ttctctgtca
     3001 ttttctttaa caagaataca ccaattacta acatggcgat tagaaccaat ccaattttaa
     3061 agttaatgaa aaagcctaag gcaactgata aaacaggtgt tactatggct tgagcactat
     3121 ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc gttgtcatca attgtctttc
     3181 gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact tgcttctgtt aaaccgctaa
     3241 ttcccttttt tctcaaatta gtctctaagc gaaacccaag gatatgagaa aataagccag
     3301 aaaaaatata aaataaagct cctaaagtta accaagcaac agtttgaacc gcataactct
     3361 tagctaaata ttcatctcct acaataatta gagactgaag aaatttgtaa atactgtaat
     3421 atccatagac tgttaatacg acagaaaaat cagaaaataa agtggctaac catccgtagt
     3481 atttcatttt agggacataa ctaaataatt ttttgtatac tttcattttt tcctcccccg
     3541 cattcaattt cttctcttat tcgtttacaa ctgttaatgc tttccgtaat agcgtaaatt
     3601 agagatgtac aaacagataa aaggtaaact acgttctatt ttttgtattt ttttaagact
     3661 tttcttccaa tcaatacacc aatcatagat aaacttgcta caactaacac tgaaatgatt
     3721 ccccatatac ctaccgcata gtggatcatt gcatccattg ttgcagcaga ttgtccttta
     3781 tctatccaat cttgacgaaa acgatctgta aagaaccata taggaatcca ctgtccggca
     3841 gcactgccta attgcattag agcccaagac gcaacttgag gaattgctcg atcatatcca
     3901 aatacagata gaataatatc agctaagatc gccatactaa tcgaactgat aaaccaaggt
     3961 aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa caattgtgta aatagtaaaa
     4021 attccttttc tagggtagtt atagctaata taaa
//
LOCUS       DADXTG010000166         4037 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.169, whole genome shotgun sequence.
ACCESSION   DADXTG010000166 DADXTG010000000
VERSION     DADXTG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.169"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            142..1602
                     /locus_tag="IWI57_002509"
     CDS             142..1602
                     /locus_tag="IWI57_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450607.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1823..3586
                     /locus_tag="IWI57_002510"
     CDS             1823..3586
                     /locus_tag="IWI57_002510"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBC2450608.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3670..>4037
                     /locus_tag="IWI57_002511"
     CDS             3670..>4037
                     /locus_tag="IWI57_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBC2450609.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAE"
BASE COUNT         1421 a          614 c          814 g         1188 t
ORIGIN      
        1 tgtgagcttg tttgactttt gtcccagttt ctttttttat tggtttttga aagggctttt
       61 ctaaataaaa aggaaacaaa aatacgattt acagatatga ttaaataaaa tcataagaaa
      121 aactggaggc gtgtatcagg aatgctggaa aattacatcg agcgaaatat atttagaaaa
      181 gtgtatctgt gtgaacaatt atttgaattc caagaaatag acatcgaaca aacagctatt
      241 tctctaaggg tgactacacc aacgatcctt cacgatctag aaagtctagc cgagtgtcta
      301 gaatattgta taaaagaaca agtaagagag aagcataagt ataaactcgt attcaaacat
      361 ggtattgctc tttctgagct cacacagttt ctgtacggtc aatcttattt tttaaagttt
      421 ttaagttatt attttaatgg tatattcacg agtacagaac tggctgatct tgaatttatt
      481 tctttgagta aagtatatac aatcaaaaaa atcgtattgg atttttttaa agaaaatgat
      541 tatttgaaaa ataaagaaat cattatccca gaatttgact cgcggaacat cttgctagct
      601 ttagtacgtt atgtcaattg ggaaggctat gaaaataaaa atccgtatgt agaagaagct
      661 tgtaacgagt tgattgatta tgtagagact cattttttta aaagaagata ttcggaagat
      721 gaaaagtcct ttatcatacg agaaatagag atcgcaatcg gaagaaaggc atttcccctt
      781 ggactgaaag aagaagataa gaaaagcgca gaaacgaagc cgttatttca attagtctgt
      841 caaggattgg aagagcaaaa aggaattcta gacttacaag aagaagatgt ctattatatt
      901 ttttctttgt tcaacagtag aaattatacc aatgagaaca tggagctgtt aagaaaagat
      961 ttcgaagttg tttataaaaa ttttgttctc aatgacctat cactgaatga attgatttta
     1021 cagattatta gtatcgctga aatcgacgat gcagaaaatt ttattttcaa gcaatccttt
     1081 ttacaatttg tccgtacgtt gtgggcagat ggacaagtat ttttaccgga aaggatctat
     1141 cttctatcag aaaaagaaca acttctgtac ggaaaaattg tagaaatatt ggaagagtgg
     1201 aaaaagaaaa atcaaatcca actaagatgg aatgaaaatt tcattcgaaa attcgtcaaa
     1261 agtctttctt tgatcaacag tgaacaagcg gggagacagg aaatagaaat ttttgttgtt
     1321 tcagaaagtt cagtcaaaca atttttttat cgaacccaat ttcatttgta tgcggaagaa
     1381 ggcatcgctg aaataaatcc tattttattt cgttcattag aagaattgcc tgatgaatgt
     1441 ctatgtgcga aaaaaagagt ggttttgtgc gatgctgcta gctatcaaaa tggattagaa
     1501 actgaaaaga caaagatcta tccaatctca ttaaaaggtg caaacatcca tttgtatcat
     1561 attgctcagg aacttcacct attaaaaaaa catatttctt gatgagacct taattaggag
     1621 accttctaca aagcttttta attgtctgta gaaggttttt atttgagtcc tgattcgtgc
     1681 gaagatctac ttatataaca agctttctct gaaatttttt taaaatgcta ttgacttaga
     1741 gtcaactcta agtagtagaa tagataaagt gtaacaaatc tattttcatt aaaagaagat
     1801 agtacggagg agagaatgta ttatgagtac agtattacat gtattggatg caatcaatag
     1861 ccccgcagat gtcaaaaaat tatctgttga ggaaatgaaa cagttggcag aagagatgcg
     1921 ccaaaaaatc ttgcaaaagg acagcgatgt aggtgggcac gttggtccga atctaggtgt
     1981 agtagaatta acgattgcgt ttcattatgt atttgattca ccaaaggata aaatcgtttg
     2041 ggatgtttcc catcagtctt atggtcataa gcttttaact ggtcgaaaaa atggttttga
     2101 aaaaggacat taccgtgaag tgagcggata ttccaaccaa catgaaagta aacatgacta
     2161 ttttacagtg gggcacacat ctacatctat cagcttagca acaggtttag ctaaagcgag
     2221 agatctgaag aaagaatcag gaaatgtggt agcatttatc ggtgacggct ccctaagtgg
     2281 tggcttagct tttgaaggat tgaacaatgg tggtgtttta caatccaatc taatcgtatt
     2341 agtaaacgat aatgagatgt ctattgatga gaatcatggc agtttatata aaaacttagc
     2401 tgagcttcgt caaaccaacg gtaaaagcag caataaccta tttaaagcaa tgggatttga
     2461 ttatcgttac gtggataatg gaaacgatct ggaaacggtg atccgtttat ttgaagaagt
     2521 aaaagatatt gatcatccga ttgtacttca cgtccatacg gaaaaaggtc gcggttatca
     2581 gccggcaatc gataataaaa tgaaatatca ttggcatgta ccatttgatc tgtccaccgg
     2641 ggaggcaaag caagtaactt ctggagaaac atttaatatg atcatcatgg attatttgga
     2701 taagaaaatg caaacaagta cagctcctat tgccgcagtc aatgcagcga taccaggcgc
     2761 ctttggactg aaaacattcc aagaaaaaca tccagatcat tattttgacg tggggatcgc
     2821 tgaacagcac tctatcactt tcgcttctgc tttggcttct cagggagtac gtccagtcgt
     2881 cttccaatca agtactttct tgcaacgggc atatgatcaa ttatcacatg atttagcgat
     2941 caacgagaat ccagtgatta ttttagtcgg tggcggaacg atctcagcta gtgaccgaac
     3001 acaccaagga acatttgaca ttccttactt atcaagtatc ccgaacatca aatatcttgc
     3061 tccagcaaca aaagaggata tgatctctat gctagattgg gcgttcgaac aaacagcgca
     3121 gcctgtagca atccgaattc ctacacatgg tgtagaacat ggtacagcta tcgcaaatga
     3181 ttatagtatt ccagcctatc aaacagtaaa taatggagga aaagtagcaa ttttagcttt
     3241 aggcggtttt cttgaattag gaaaagaaac agtaaaagct tttagaaaaa aaggaatcga
     3301 agcaacgttg atcaatccag gaacgatcac agaaaatgac gtagaaacat tagataaatt
     3361 aaaagaaaca cacgaattag tcatcacatt agaagatggt agtttgtcag gaggatttgg
     3421 cgaaaatatc agtcgttatt atggtgattc ttcaatgaac gtattaaatt ttggtgcaga
     3481 gaaagaattc acggatagcg ttcctgtagg gcagctttat gaacgttatc atttaacacc
     3541 aaaacaaatc gttgaagata gtctgcgtgt cttgaatatt caataattca ttatgctcac
     3601 catccagata attgtttata ctagtatctg gatggttttt tcattataaa aagcaaaggg
     3661 gtggatgtta tgtatcagca agtagaagaa tatgtcaaaa agttccaaga aaaatatcct
     3721 agttatcaat cagaaaaatt aggtttcttt tcatttgggg atacagaaaa aatggcagac
     3781 gagttgagcg aactagttgt aaaaaagata aagactggta catcaagtgg ttacgatctg
     3841 tataaacaag agaatgagcc tttaccaaaa gttggtcaat tagacgtggt cctgaatgga
     3901 aaaaatgaac cagtttgtat catcaaaaat ataaatgtga ccatcttgcc ttttagtgaa
     3961 gtcacgaaag agcatgcttg gaaagaagga gaaggggatc ggacattagc ttattggcgg
     4021 aaagtccatg ctgagtt
//
LOCUS       DADXTG010000167         3997 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.170, whole genome shotgun sequence.
ACCESSION   DADXTG010000167 DADXTG010000000
VERSION     DADXTG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.170"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(83..823)
                     /gene="truA"
                     /locus_tag="IWI57_002512"
     CDS             complement(83..823)
                     /gene="truA"
                     /locus_tag="IWI57_002512"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBC2450610.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1014..1649)
                     /locus_tag="IWI57_002513"
     CDS             complement(1014..1649)
                     /locus_tag="IWI57_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBC2450611.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1796..3037)
                     /gene="sstT"
                     /locus_tag="IWI57_002514"
     CDS             complement(1796..3037)
                     /gene="sstT"
                     /locus_tag="IWI57_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBC2450612.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3235..>3997
                     /locus_tag="IWI57_002515"
     CDS             3235..>3997
                     /locus_tag="IWI57_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450613.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFS"
BASE COUNT         1313 a          809 c          623 g         1252 t
ORIGIN      
        1 atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt
       61 ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg
      121 cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt
      181 ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt
      241 tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag
      301 ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa
      361 tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact
      421 cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa
      481 cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt
      541 cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt
      601 ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc
      661 cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata
      721 ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat
      781 atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct
      841 gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg
      901 cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa
      961 aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt
     1021 ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa actgattctt
     1081 tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt
     1141 ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg
     1201 tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca
     1261 ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca
     1321 tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg
     1381 gctgctgtaa ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca
     1441 ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt
     1501 acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga
     1561 taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag
     1621 aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt
     1681 ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca
     1741 tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac
     1801 gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg
     1861 cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca
     1921 ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat
     1981 tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac
     2041 ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag
     2101 gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg
     2161 caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca
     2221 atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa
     2281 agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt
     2341 taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga
     2401 tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg
     2461 attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt
     2521 gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa
     2581 aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag
     2641 attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt
     2701 gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta
     2761 caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct
     2821 tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa
     2881 ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa
     2941 atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac
     3001 gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta
     3061 acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac
     3121 aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt tgttttttga
     3181 gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt
     3241 ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc
     3301 ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca
     3361 gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa
     3421 acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa
     3481 atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc
     3541 acactagttt gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat
     3601 tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg
     3661 cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt
     3721 aaatatctac tattattttt aacaattgga ttagttggct gctctactct atttaataaa
     3781 gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt atttttaatg
     3841 ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta
     3901 tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc
     3961 atcagaggaa atacagatac agcgctgagg ttttcta
//
LOCUS       DADXTG010000168         3980 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.171, whole genome shotgun sequence.
ACCESSION   DADXTG010000168 DADXTG010000000
VERSION     DADXTG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.171"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(15..1271)
                     /locus_tag="IWI57_002516"
     CDS             complement(15..1271)
                     /locus_tag="IWI57_002516"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBC2450614.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(1383..1588)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1851..3152
                     /locus_tag="IWI57_002517"
     CDS             1851..3152
                     /locus_tag="IWI57_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBC2450615.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3221..3820)
                     /locus_tag="IWI57_002518"
     CDS             complement(3221..3820)
                     /locus_tag="IWI57_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2450616.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         1199 a          790 c          680 g         1311 t
ORIGIN      
        1 aaacttacta aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga
       61 ataaccacaa acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta
      121 acgcggtcac cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta
      181 gatggttcaa tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt
      241 tcaacgttcg gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata
      301 tttccagaga acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc
      361 acaaatcttg taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc
      421 tctacttttt tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag
      481 tatttcacta catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa
      541 gttttcttcg gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca
      601 gccttcagca tcaatgggat cgttaaaccg aacgcttctg cttctggtcc ttctaatttt
      661 cggattaggt ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat
      721 tgcacatcgt atgttttatg cagatgcaaa aagtcgatag attgcaggat ttggtaagta
      781 aactctgtaa atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc
      841 gtgttgatgt tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac
      901 aaccagtcgt agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt
      961 tgattggaaa gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct
     1021 gttttgcggc cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga
     1081 tgatggcctg ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc
     1141 atcgaatcac cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc
     1201 tcgcgaagtc tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca
     1261 ataatattca ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca
     1321 tatgtaatta attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata
     1381 agccaaggga cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc
     1441 tctcgaacat ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc
     1501 gctttaaagc atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca
     1561 actacttcgt ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac
     1621 tttttctttg tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga
     1681 atccgctcta attccgaaca ttttattttc aattattgtt aaaagaaata actttttctg
     1741 tattttttct gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta
     1801 caataaggaa tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa
     1861 agcaaaaaat tattttagga gccacttctg ctttaactat cgttatttta attctaagtt
     1921 tgttttttgt catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa
     1981 cagaaaaagc aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc
     2041 tggctcaaag ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg
     2101 gatctactga aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg
     2161 actggtctga tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc
     2221 aaaaagcctt ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag
     2281 aattcaaccg ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg
     2341 atttgatcca aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag
     2401 gaaaaaaacc attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt
     2461 ccggttttcc tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag
     2521 agaaagataa agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca
     2581 agaaacatcc tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca
     2641 acggagtttt aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa
     2701 aagagattaa agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg
     2761 cttcccatac atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag
     2821 acaatgaatt gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt
     2881 atccatttgg tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg
     2941 cttacttgaa gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt
     3001 ggggacaatt aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt
     3061 ttcaatctga tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg
     3121 tctatgacaa agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc
     3181 cgaactgaca ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga
     3241 ttcccttgat caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct
     3301 ccggcttaga aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat
     3361 atttcaaatc cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga
     3421 aactttcttg cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat
     3481 agtatgtcat caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt
     3541 tttcactcat ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg
     3601 cttcttgccc taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc
     3661 tgccaaaata tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca
     3721 ttgtcactcc agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct
     3781 ctttcttttt ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt
     3841 ttaaactttt ataatatgta tagttcaaat ttattctgag gtttattata actatctatt
     3901 gagaaagtat caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa
     3961 gaatattatg tactaaaata
//
LOCUS       DADXTG010000169         3916 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.172, whole genome shotgun sequence.
ACCESSION   DADXTG010000169 DADXTG010000000
VERSION     DADXTG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.172"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(25..1530)
                     /locus_tag="IWI57_002519"
     CDS             complement(25..1530)
                     /locus_tag="IWI57_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBC2450617.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="IWI57_002520"
     CDS             complement(1527..2213)
                     /locus_tag="IWI57_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBC2450618.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IWI57_002521"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IWI57_002521"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBC2450619.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1321 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atattt
//
LOCUS       DADXTG010000170         3900 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.173, whole genome shotgun sequence.
ACCESSION   DADXTG010000170 DADXTG010000000
VERSION     DADXTG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.173"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            325..639
                     /locus_tag="IWI57_002522"
     CDS             325..639
                     /locus_tag="IWI57_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBC2450620.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            655..1041
                     /locus_tag="IWI57_002523"
     CDS             655..1041
                     /locus_tag="IWI57_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBC2450621.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1070..2455
                     /locus_tag="IWI57_002524"
     CDS             1070..2455
                     /locus_tag="IWI57_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBC2450622.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2458..2610
                     /locus_tag="IWI57_002525"
     CDS             2458..2610
                     /locus_tag="IWI57_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2450623.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2633..3838
                     /locus_tag="IWI57_002526"
     CDS             2633..3838
                     /locus_tag="IWI57_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBC2450624.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1290 a          712 c          835 g         1063 t
ORIGIN      
        1 aaaatctagt tatccgcata aaaactggac ttatcacact ttatcaaggt caaaaccact
       61 caatttacta ctaatttact acttatgaat gagctttgat acgacgattt atccttgaaa
      121 agtgaagata taaagatact tccaataaaa tttgaatatt taataggtag acacttcaaa
      181 aaatgaggtg tctatttttt tacccgattt tgaaaggaag tgaacttatg aaaacaaaaa
      241 atcaagaatc aaaaggtcgt tccccactct ttaagaccat caaacattca ttcagccaat
      301 aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg tgattcccaa catggaaaaa
      361 acattcggca atttagaatt tgctggcgag gataaagtcg ttcagcgaag aatcaacgga
      421 cggctaactg tcttatcaag aagctataat ctctattctg atgttcaaag agcagatgat
      481 attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt tcggctttga ggaacgtgtg
      541 aagttagtca atccacgtat taccgcagag ggctacaaaa tcggcactcg tggttttaca
      601 aattaccttt tacatgctga cgacatgata aaagaataaa gaaagagagg aaaaatgatg
      661 agattagcaa atggcattgt attagataaa gacacgactt ttggagaatt gaaattctct
      721 gctctacgtc gtgaagtgag aatccaaaat gaagacgggt cggtttcaga tgaaatcaag
      781 gaacgtacct atgacttaaa atccaaagga caaggacgca tgattcaagt aagtattcct
      841 gccagcgtgc ctttgaaaga gtttgattat aacgcacggg tggaacttat caatcccatt
      901 gcggacaccg ttgctactgc cacctatcaa ggagcagatg ttgactggta tatcaaggca
      961 gacgatattg tgctgacaaa ggattctagt tcattcaaag ctcaaccaca agcaaagaaa
     1021 gaaccgacac aagacaaata gtcgctaggt agaaaggaga ctttttcgca tgaaacagcg
     1081 tggtaaaagg attcgcccat ctggtaaaga tttagtcttt cattttacga tagcgtcact
     1141 cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg aagacaatcc agcagatcaa
     1201 ctggcaggat tttaacctat cacaagcaga taagattgac attccctatt taattatcag
     1261 tttcagtgtc gcaattctta tctgcttgct ggtagcgttt gtattcaaac gggttcgcta
     1321 tgatacggtt aaacaacttt accaccgtca aaaactggca aagatgatac ttgaaaacaa
     1381 gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt aaagatagtg ctggtcgtac
     1441 aaaggaaaag ataacctact tccctaaaat gtattatcga cttaaaaatg gcttgataca
     1501 gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa ctcttacact tggaaaagaa
     1561 attagagagt ggcttgtact gtgagctgac ggataaagag ttaaaggatt cctatgtgga
     1621 atatactttg ctctatgaca ccatagccag tcgtatttct attgatgaag tagaagctaa
     1681 agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa tatgataagc tccctcatat
     1741 gttgattgct ggtggtacag gtggcggtaa aacttacttt atactgacac tgattgaagc
     1801 cttgcttcat acagattcaa aactgtatat tcttgacccg aaaaatgctg accttgcgga
     1861 cttaggttct gtgatggcaa atgtctacta tagaaaagaa gacttgcttt cttgcattga
     1921 aacattctat gaagaaatga tgaaacgtag tgaggaaatg aagcagatga agaactataa
     1981 gactggcaaa aattatgctt acttaggtct cccggcacac ttcttaatct ttgatgaata
     2041 cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc gcagttatga ataagctgaa
     2101 acagattgtc atgttaggtc gtcaagctgg cttctttcta atactggctt gtcaacgtcc
     2161 agacgcaaaa tatttaggcg acggaatccg tgatcagttt aatttcagag tggctttagg
     2221 tcgtatgtct gaaatgggct atggcatgat gtttggcagt gacgtacaaa aggatttctt
     2281 cttaaagcga atcaaaggtc gtggctatgt tgatgtagga acaagtgtca tatcagagtt
     2341 ttatactccc cttgtaccaa aaggatatga tttcttggag gaaattaaaa agttatccaa
     2401 cagcagacag tccacgcagg cgacgtgcga agcggaagtc gcaggtgtgg actgatcttg
     2461 ctggctggtg tggcaatagc cacgccagca cttaaccccc cgtatctaac aggggggtac
     2521 aaatcgacag gaaacagtca aaaaaacatt agaaaatcct ttggttacaa gggatttaca
     2581 aaatttcagc gtatgtcaaa tgggctttaa aagttgacat acgccttttt gattggaggg
     2641 atttttactg aatgaacaaa cttggttaca gcatttaaaa gaaaaacgct tggcttatgg
     2701 actatctcaa aaccgtttag ctgttgcgac tggtattaca aggcagtatc taagcgatat
     2761 tgaaacagga aaagtcaagc catcagagga tttacagcag tccctttggg aagctctgga
     2821 acgcttcaat cccgacgctc cccttgaaat gctgtttgat tatgtaagga ttcgctttcc
     2881 gacaacagac gtacagcagg tggtcgaaaa catcttacaa ctgaaactgt cctattttct
     2941 tcatgaggac tatggtttct attcttattc agagcattat gctttaggcg acatattcgt
     3001 cctttgctcc catgaactgg acaaaggagt tctggtggaa ttgaaaggtc gtgggtgcag
     3061 acaatttgaa agctatcttc tggcacaaca aagaagctgg tatgagttct ttatggacgt
     3121 tttggtggct ggcggtgtga tgaaacgcct tgaccttgcc attaacgata agacagggat
     3181 tttgaatatc cctgtactca ctgaaaagtg ccaacaggaa gaatgtatct ccgtcttccg
     3241 cagttttaaa agctatcgca gtggcgaact ggtacgcaaa gaggaaaagg aatgtatggg
     3301 aaacaccctc tatatcggtt cattacaaag tgaagtttat ttctgtatct atgaaaagga
     3361 ctacgagcag tacaagaaaa atgatattcc cattgaagac gcagaagtaa aaaaccgttt
     3421 tgagattcga ttgaaaaatg agcgtgccta ttatgcagtc cgtgatttac tcgtctatga
     3481 caatccagag cataccgcct ttaaaattat caatcggtat atccgttttg tagataaaga
     3541 cgattccaaa cctcgttctg attggaaact gaatgaagaa tgggcttggt ttattgggaa
     3601 caatcgtgaa cgattaaaac taaccacaaa accagagcct tactccttcc aaaggacgct
     3661 gaactggcta tctcatcaag ttgccccgac cttaaaggtt gcgattaaac ttgatgaaat
     3721 caaccagacg caggttgtaa aagacattct cgaccatgcg aaactgacag accgacacaa
     3781 gcagattttg aagcaacagt cagtaaaaga acaggacgtg ataacaacaa aaaaataact
     3841 caaatacaaa ttcattgaat atagagagga gaacattttt atgaattttg gacaaaacct
//
LOCUS       DADXTG010000171         3899 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.174, whole genome shotgun sequence.
ACCESSION   DADXTG010000171 DADXTG010000000
VERSION     DADXTG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.174"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..223
                     /locus_tag="IWI57_002527"
     CDS             <1..223
                     /locus_tag="IWI57_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450625.1"
                     /translation="IVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGY
                     WYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            233..436
                     /locus_tag="IWI57_002528"
     CDS             233..436
                     /locus_tag="IWI57_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450626.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            647..1231
                     /locus_tag="IWI57_002529"
     CDS             647..1231
                     /locus_tag="IWI57_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2450627.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1602..2927
                     /locus_tag="IWI57_002530"
     CDS             1602..2927
                     /locus_tag="IWI57_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBC2450628.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2920..3270
                     /locus_tag="IWI57_002531"
     CDS             2920..3270
                     /locus_tag="IWI57_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450629.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3267..3446
                     /locus_tag="IWI57_002532"
     CDS             3267..3446
                     /locus_tag="IWI57_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450630.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQLISMNPFLFLQIGVDTRI
                     MRVLNYYCKRNNKRK"
     gene            3458..3748
                     /locus_tag="IWI57_002533"
     CDS             3458..3748
                     /locus_tag="IWI57_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBC2450631.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT         1392 a          581 c          752 g         1174 t
ORIGIN      
        1 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
       61 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
      121 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
      181 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
      241 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
      301 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
      361 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
      421 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
      481 ttgaaagtgt tcataaaatc acccatttct ggacacttcc tttttttgct ttatttttca
      541 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
      601 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
      661 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
      721 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
      781 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
      841 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
      901 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
      961 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
     1021 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
     1081 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
     1141 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
     1201 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
     1261 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
     1321 tattatcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
     1381 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
     1441 ttcaaattaa ttagtaaagt gatagaataa attgatcata caaatcataa gaagaagcag
     1501 ggtgatagca tacctatgct tcttttttta tgtatttagc tatttcttct ttacaacatc
     1561 gaacatatgt ttgtataatg tggaagaatg gagtgttatt tatgatggat tattcaaaag
     1621 agccagtaaa tgattacttt ttaatcgata tgaaaagctt ttacgcaagt gtcgaatgta
     1681 tagaaagaaa tttagatcca ttaacaacag aacttgttgt tatgagtcga tctgataata
     1741 ctggttcagg attgatatta gcttcttctc ctgaagcaaa aaagcggtat ggtattacaa
     1801 atgtgagtag accacgtgac ttaccacaac catttcctaa aacactacac gttgttccac
     1861 cacgtatgaa cctatatatc aagcgaaata tgcaggtaaa taatattttc agaagatatg
     1921 tggctgatga agatctactg atttactcga tcgatgaatc gattttaaaa gtgacaaaat
     1981 cactgaatct ttttacgact gaagaaacac gaagtcagcg aaggaagaag ctcgctcaaa
     2041 tgatccaaga acgtattaaa gaagagttag gtttgattgc tacggtaggc gtcggcgata
     2101 atcccctttt ggctaaatta gccttagaca atgaagcaaa gcataatgaa ggatttattg
     2161 cggaatggac gtacgaaaac gttcctgaga aagtatggaa tattcccgaa atgacggact
     2221 tttggggaat tggttctcga atgaaaaaaa ggctcaacca aatgggaatt ttgagcatta
     2281 gagaccttgc taactggaat ccttatacga ttaagaatag attaggtgtg attggattac
     2341 agctgtactt ccatgctaac ggaatcgatc gaacagatat agcaatcccg ccagaaccga
     2401 caaaggaaaa atcttatggt aattcacaag tgttaccacg agattatact agaagaaatg
     2461 aaatcgagct ggtggtaaaa gaaatggcgg aacaagtagc cattcgtatc aggcagcata
     2521 attgtaaaac aggatgtgtt catttaaaca ttggtacgtc gatcttagaa acgagaccag
     2581 gattttcaca ccagatgaaa atacctatca cagataatac aaaggaattg cagaattatt
     2641 gtcttttctt gtttgataag tattatgaag gacaagaagt aaggcacgtt ggaataacct
     2701 atagcaagct tgtgtacacc gattcgcttc agctggattt atttagtgat ccacaaaagc
     2761 aaattaacga agaaaattta gataaaatta ttgacaagat tcggcaaaaa tatggtttta
     2821 cgtcgattgt acatgcttct tcaatgttag aaagcgctag aagcattact agaagcacac
     2881 ttgttggtgg tcatgcgggc ggaaatgggg gaattaaaaa tgattgatta caatgatcgt
     2941 ggacgtttaa agtgggcagg gttttattta tctgatcata ctgaagaaat tgatgccgac
     3001 aatgaacaac gaaccagtca aaatttagca aaagaacaaa tgacgactga agagattgcg
     3061 gaagtattga acgctgcagt cataaaaaat agatcagtat ctattcaaaa agaagaacga
     3121 aacgctgaag gatactatcc agaagatatt gttggcaaga tcttaggtag tgatgagctg
     3181 gggatctacg ttggaaaaga caaaattcac tacgacgaaa tacgtcatat cgaattttac
     3241 caggataata aatggagtcg attagaatga acattatcca gcaatatgag ctaaagtata
     3301 taacctttga tcagctatca gaagagattt ggggttatgg ccaacttatc tctatgaatc
     3361 cttttttgtt tttacaaata ggagtagata ctagaataat gcgtgtgtta aactattatt
     3421 gcaaacgcaa taataaaaga aagtaggctt acgcaccatg tctatcaata catttgttta
     3481 ctcgcacccg atcaatgttt acattatcaa gaatttagga atcactgtag aacaattttg
     3541 tgagttatat gcctacccac aaggaacggt tgcaagctgg ataacccgac aacgtagaat
     3601 aaaatcatta cctgcttcat tcgtttatga tttaagcctg gcctctagtt taaacatgtc
     3661 tgatgtttat gaaaagctcc tgtctttaga aaaagaatat gattccttca agattaaaca
     3721 tgataaaaaa ataaaaaaac atatataata tcaatcacaa ttgtataata ttacgatatc
     3781 ttaatatcat gatatcgtaa tattgttttt ttctaagaaa atgcactata agaagggaat
     3841 tattagtaac aatatagaca gtttaataat gtttaaatta ttctagttgc tgctcgcaa
//
LOCUS       DADXTG010000172         3778 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.175, whole genome shotgun sequence.
ACCESSION   DADXTG010000172 DADXTG010000000
VERSION     DADXTG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.175"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(56..967)
                     /locus_tag="IWI57_002534"
     CDS             complement(56..967)
                     /locus_tag="IWI57_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBC2450632.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1424..1852)
                     /locus_tag="IWI57_002535"
     CDS             complement(1424..1852)
                     /locus_tag="IWI57_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450633.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1880..2518)
                     /locus_tag="IWI57_002536"
     CDS             complement(1880..2518)
                     /locus_tag="IWI57_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBC2450634.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2522..3292)
                     /locus_tag="IWI57_002537"
     CDS             complement(2522..3292)
                     /locus_tag="IWI57_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBC2450635.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1135 a          714 c          562 g         1367 t
ORIGIN      
        1 atttagatga acagttttat tgatttaaat agttcatatt ttttatttat accgcttatt
       61 tactacttgc tgtactagaa tctgaagtga atgtactcaa aacattttca aagtctttgt
      121 ctttgatttt tacattagcg tctttcaata cttcgccaat tactttattc tggaaggtac
      181 tgtcacttaa ttttgtgtct gttgcaattt ctttcaattc ttttttgtat ttatccatgt
      241 cgttgccttt gttttggttt ttcaccattt taaccacata atattccgta gtataagttg
      301 aagcattggt agaagtgatc acatcagaaa cttgaccatc tttcaactta aaggctgctt
      361 ctttgacttc tgccggaacc gttgtagtag tggaatcaaa tttcactttt ccgccatctt
      421 cttttgtcgt agtatcagtt gacttgtctt tggctaattt agagaaatcg tctcccttat
      481 cagcagcttt cttcacatct tttgcatcat cttcacttgc tacttggata atttgagctt
      541 ccacttctgg atggaaagat tcccaagccg tttttaaatc ttcatcggtc aatttgatat
      601 gtttctttag accttcttga aaggctagac tttgtttgat actgtctttg aatgtttgtt
      661 ctgtgtaacc tgcagatttc aattgtgaat caaatgaatc acccaattgt ttctttgttt
      721 gatcatattg agaatcgatt tctttggttg atacgtcatc tccatattta tcttcaaata
      781 cttgataaac gatcaactgt tgcacagcgg aatctgctcc ttgcattttt ttcaagctat
      841 ccattacttc ttgttcgctg attgtaccgc ctttataact aacggctgtt ttttcaccgg
      901 cattcgtaca gcctgacaat aagccagtgc ctagtacgat ggcaagtgct aaaaatcttt
      961 ttttcattta tcaattctcc tacttttcgt tttttgcgaa ttgctaaaaa caaaggtgtt
     1021 caagatgaaa atcatcaaga acacctttac taatctgctc gatacgtcct gaatagatat
     1081 aaggctacac aatccagaaa gttcgctcag cagaataaat gtcaagtagc acatcacacc
     1141 ttaatatact acttcttttg ttaccgtttt tcaagtctat cttgcgttcc gtatttaacg
     1201 taactaattc ttagaacaaa tgttatcgat tttgtttttc ttcttccgct gctgggatat
     1261 tcaaatcaga cacttttatg ttcgattttc cagacatttt tttgttgttt ttattatgat
     1321 atatttttat acaactaaaa atgaatatat taaaactacc aaaaataact tttaacgctg
     1381 tttcattata tgttttttct attttatata aacttttgga tacttaatac caaccggttg
     1441 gattgtacca acatgagaat gtgtcagttc tatttcctgt cgcacttgca taggcccata
     1501 ctccttttgt tgcatttgct gatcgaatag agtttccaac tttcgctttt gcaccgtgac
     1561 gattatagac acgactatag tggttagaat gacctgtttt tctcgtacaa gttgtatcac
     1621 tataataaac ccatcttcca gagctatgat taattgctgt tctagaaatt tcccggtcta
     1681 ctggtaaatt tgacttaata atattacctt tagcatcaaa atgaatctct cctggcttta
     1741 aagatttgta atagatttca ccgtgaactg aatttgcttc cacttcagat gacatagcgt
     1801 ttcctccaac aaaacctatc cctagtgcac aaataataac tgcttttttc ataaaacgac
     1861 atcctttcct ttttgatttt taaagcatga tcttcttgtt tcctattttg gtcagatatg
     1921 gatcatgcgt gactacgata actaacgtac cttgggaagc ttgttgcgat aagatttcaa
     1981 ctactttatc cttatttctt ttatccaatg acccagtcgg ttcatcggct aaaatgattt
     2041 tgctattctt caataagact ctcgcacagg cgattctttg cttctcacca ccagataatg
     2101 tgtagatagg cgtacttagc ttcttgttta tatttacttt tttcaatgcc tcatactttg
     2161 ctgttcgctt atctgaaaca tttaaattcg aaaacttcaa tccaatatct aaattaaaat
     2221 cgattgtttc atattcaatc aacccaaaat cttgaaaaag aaaagatatg gtatttcttt
     2281 ttatcaagcg cttctctctc ccagttaatt gtttcgtctc ttgatcgaac aaatagaatt
     2341 gtcctgaact gaactgatca ttcaatccta atatattcaa taacgtactt ttacctgctc
     2401 cactttctcc aacaacagtc acaagatccc cagcatccgc agtgaaacga tccaaatgaa
     2461 gaatcgtttt gtttcgaatg atttttttcg caccttttat cactatcaac ggctccaatt
     2521 gttaacctcc attcatcagc aataactgac ttttcttttc tgtagacaaa ataaaaacaa
     2581 ctgaaagaat aaaatcaatg aagctaaaga aaaaaatatt aaaaagatat ttaggctctc
     2641 tttctataag aaaaaggaag attacacttg tcgttcccca tttgatgagg ttcatcatga
     2701 agaaatcttt atacgtttca aagagcgaat aaccacttga acgaaagata gcgattttcc
     2761 gacaattgta gacaaaatat atgcataaca tgacatagct caacaagaag aaagaaaaga
     2821 aagtgagtaa aaatatctgt atcaagtaat gaaatgatct gcttaacact ttgacagcta
     2881 tctcttcagt ctgagtatac ggaaatgata actgcaagtt atcacgtaac tgactttctt
     2941 caatcagctc aaataacctt tctttcgaca aagagcctat cttgatcttt aacggtggat
     3001 agatttctcc gctgagaata tttctttcca aatacatact gtttttcaat gtcagtattt
     3061 caacaaccgg ataatgttcg atccagggga tagaaggaat aaatgtataa atgggttgat
     3121 tgttttttat aaatagaaat ttcggttctt tttcactgat ttcttgataa tattctgtta
     3181 tttgcgccag gtcatttttt aaatctgtat gtctatatcg ttcagggatc aatataacag
     3241 ggtgcgtttc tttttcggtt atttggatca accttccaga gacatcaatc aaaggaaatt
     3301 tcttaagata attcagattt atttttatcc ctctgccaaa ctgttcattt tcttcattca
     3361 agtaatctga tacattgact aatatacttc cgtttttatt caaagtttca tataattcct
     3421 gatctgctgc tgatgcaaaa cgattggaat aaataaaatc cacgtggttt ttcccgatgg
     3481 ttactggata aaatacatga tagtcctcac cagcgatttc ttttggtggt tcatgggaaa
     3541 cagctaactg tgaagaagaa aaaacgatag ttgctaattc gacattggca acggtcaaaa
     3601 gaagagaaaa caacaatcca aatgtaaaaa gaatgtattg gatatagttg aacgcttctc
     3661 ttaacgaagc gattttttca actaataaaa gagaaatcaa agagatacca tatattccgc
     3721 tgaacaagaa aacgtacatc tggaacaaat aaagaaattg ctccttataa tacataaa
//
LOCUS       DADXTG010000173         3763 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.176, whole genome shotgun sequence.
ACCESSION   DADXTG010000173 DADXTG010000000
VERSION     DADXTG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.176"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            208..1197
                     /gene="galE"
                     /locus_tag="IWI57_002538"
     CDS             208..1197
                     /gene="galE"
                     /locus_tag="IWI57_002538"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBC2450636.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1209..2693
                     /gene="galT"
                     /locus_tag="IWI57_002539"
     CDS             1209..2693
                     /gene="galT"
                     /locus_tag="IWI57_002539"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBC2450637.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2717..3697
                     /locus_tag="IWI57_002540"
     CDS             2717..3697
                     /locus_tag="IWI57_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2450638.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1279 a          672 c          817 g          995 t
ORIGIN      
        1 acaaaagaga aaaaaaggct agttttacgt ttgtttgtaa acctttgcaa aaaatccttg
       61 aaaatgttta ctaaatgttt tattataaat ttaccatatg aatttacgaa attttacttt
      121 gtttgttttc accataaaag taaacttcgt tttttaaatt atatagaatc aacaaacaca
      181 attttactaa tcaaaggagt gttttttatg acaattttag tactaggcgg agccggttat
      241 atcggttcgc acgctgtaga ccagctagtt caaaaggggt atcaagtagc tgtggtagat
      301 aatttgctca caggtcacaa acaagcagta catccagatg ctcattttta tgaaggagac
      361 atccgggata aagaatttct acgttccgta tttgaaaagg aaccgattga aggagtaatc
      421 cattttgctg caagctcttt agtaggagaa tctgtcgaaa aaccattgat gtatttcaac
      481 aacaacgtat acggtatgca aatcttactt gaagtcatgc acgagttcaa cgtgaataaa
      541 atcgtattct cttctactgc agcaacttat ggagagccga aagagtctcc aatcacagaa
      601 gatacaccag ctaatcctaa aaatccgtat ggtgaaagta aattgatgat ggaaaaaatg
      661 atgaagtggt gtgaccaagc atatggcatg cgctatgtcg ctttgcgcta cttcaatgta
      721 gccggagcaa aagcagatgc atctatcgga gaagatcata caccagaaac gcatcttgtc
      781 ccaatcattt tacaagtagc tcttggtcaa agaaaagcct tggctgttta tggagacgat
      841 tatgatacac cagatggaac atgtatcaga gattatgttc aagtagaaga tttgattgct
      901 gcacatattt tagctttgga atacttgaaa gaaggaaatg aaagcaactt cttcaattta
      961 ggcagcagca aaggatactc agtcaaggaa atgctagaag cagctagaga agtgacagga
     1021 aaagaaattc cagcagaaat agcaccaaga agagctggag atcctagccg attagtcgct
     1081 tcaagtgaaa aagcgagaga ggttctgggt tggaaacctg aatacacaga tatcaaagca
     1141 attatcaaaa cagcatggga ttggcatgtc agccatccaa atggttatga agaatagggg
     1201 aatgagatat gtcaatcagt caaacgattt gtgattttgc cacacttgcg atcgaagctg
     1261 gcggatggat ggaaatggac cgcttatatt tacagaatcg tcttttaggg atggtaggag
     1321 aagatgctct agaaagcatt gaagtaagac cagttaagga agaatctgta gacttattgg
     1381 atcagcttgt cgctaaggca agagaaaatg gtgttctttc agaattgcca gcagaacaag
     1441 agatttttga agcgcaatta atggattttc tcacaccgcc accttcagtc gtcaatgcat
     1501 tctttgcaca gcactattcg aaaagcccag aggaagcaac tgagtatttc tttaatttaa
     1561 gtaagaaaaa tgactacatc aaaacaagag ccatcgcaaa aaacatcaaa tttccggcag
     1621 agtcggagta tggtgaactg gaaatcacaa tcaatttatc taagccagaa aaagatccaa
     1681 aacaaatcgc agcagaacgc gaggcggtaa aagtagacta tcctacctgc atgctttgca
     1741 tggaaaacga aggatataga ggtcgcttga actatccagc ccgcaccaat caccggatta
     1801 ttcgaatgaa tctagacggt gaaagctggg gattccaata ttctccttat gcttattaca
     1861 atgaacattc gatcatttta tcagaacaac accgcccgat gaaaatcaca aaggaaacat
     1921 ttgctagact gcttcggatt actgaagtct tgcctcatta ctttgttggt tcgaatgctg
     1981 acttgccgat tgttggagga tcgattcttt ctcatgatca ttatcaggca ggacgtcatg
     2041 aatttcctat ggcaaaagca ccgatagaaa aacttatcaa actagcagat tatccgcaag
     2101 tcatcgcagg agtcgtgaaa tggccgatgt ctgtgatccg tttgcaatca acagagaaaa
     2161 aattgttgat tgaagcagct aactcagtat tggaaaagtg gaaagcatat tcggatgaaa
     2221 cagtatctat cagagctttt tcagcagatg gtacaccaca ccatacgatt acgccaattg
     2281 cacgaagaag ggacaattac tttgagttgg accttgtcct tcgagacaac aatgtttcag
     2341 aagaacatcc tgatgggatt tttcatccac ataaagatgt ccaacatatc aaaaaggaga
     2401 acattgggtt gatcgaagta atggggcttg ctgttttacc acctcgtttg aaaccagaac
     2461 tagtagaagt agagcatttc ttattaggag aagaaaacca gatggctgaa tatcatcgtc
     2521 cgtgggcaga acaattaaaa aaagaacatc ctgatttgac gaaagatacg gtcaccgatg
     2581 tcgtacaaaa atctgtaggt caggtttttg cacgtgtact ggaagacgct ggagtattca
     2641 agcgagatga aaaaggacag gcagcgtttg aacgatttac tgcaatttta taaacgtaaa
     2701 aaaggaggag agacaaatgg caacaattaa agacattgcg cagttggcag gagtttctcc
     2761 tgctactgtt tcaagagttc tcaactatga tcctgatctt tccgttggct tagccacaaa
     2821 gcaaaaaatt tttgaagcag cagaacgctt aaattatacg aaacatcgta aaaatgcagc
     2881 aaatactaag caaacgcttc gcctgatcca gtggtacgac gatacggaag agcttgaaga
     2941 tctttattat ttatctattc gtctaggaat cgaaaaaaag gcagaagaag caaacgttac
     3001 tttattgaaa gaaacgtggg attccattag tgatcttccg acagatggaa cgatcgcact
     3061 aggaaaattc gacgaagctc agttattaga aatgaaagga tcccaaaatg ccttgctttt
     3121 ggtagattct gacggcactt ctcaaggaat cgactctcta gttgtcgact ttaagtcaag
     3181 tgttcaaaaa gtcattgaac attttgtctc ccataaagcg caacatatcg ctatgcttgc
     3241 tggaacagaa tatacgaaga aaaaccatca gcggctaaaa gatcccagaa ttatggcatt
     3301 tcaacaagtt ttggcagaaa aaggctacga cgaaggtcca attatcgaag cagatttttc
     3361 tgtcgagtca ggatatcaag cggtgaagca gtatttaaaa acgaatccgc atgttcctga
     3421 tgcgcttttt gcagctaatg atgcattagc gattggcgca ctgaaagcga ttcaagaaaa
     3481 aggtttagtg gtaccagaag atatttctgt gatgggtttc aatgatgtca gtgtagcgaa
     3541 atatgtaacg ccctctcttt ctactgtaaa agtttatacc gaatggatgg gagagctagc
     3601 agtggagacg attcttcaat taagtaattc caaagcaccg gtttcaagaa agattattgt
     3661 ggaaacagag ttgattattc gagaatcaac gaaataaaag atggaaaagc tgtggggaaa
     3721 tgaagtgata ccgcagcttt tttgtgtttc aggaagggac aaa
//
LOCUS       DADXTG010000174         3749 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.177, whole genome shotgun sequence.
ACCESSION   DADXTG010000174 DADXTG010000000
VERSION     DADXTG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.177"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..513)
                     /locus_tag="IWI57_002541"
     CDS             complement(55..513)
                     /locus_tag="IWI57_002541"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBC2450639.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(684..1829)
                     /locus_tag="IWI57_002542"
     CDS             complement(684..1829)
                     /locus_tag="IWI57_002542"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBC2450640.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1842..2759)
                     /locus_tag="IWI57_002543"
     CDS             complement(1842..2759)
                     /locus_tag="IWI57_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBC2450641.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRVIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3187..>3749
                     /gene="efp"
                     /locus_tag="IWI57_002544"
     CDS             3187..>3749
                     /gene="efp"
                     /locus_tag="IWI57_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBC2450642.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1150 a          723 c          722 g         1154 t
ORIGIN      
        1 aaatacgaag ataagatagc tatattgctt ctcatcttta cgttctttta gaaactatgc
       61 ttctacttca gaccattcat cgcgttttgc taaaaattca cgagccaaag cttgtgcacc
      121 ttctagtgta tggctggcag cccagccaca ttgtgtttca ttgcttgcag gtacttcagt
      181 agcttcaaga acatctttca ttgttgcttc aagaacatct aaaatgtctt cgtagctttc
      241 atgattcaat acagtcaagt aaaagcctgt ttggcatccc attggagacc agtctacgat
      301 gtaatctgcg tgattgcgga ttagttcggc agtcaagtgc tcaagtgaat gtagacttgg
      361 catgtccatg tgttctttat ttggttgttt aaagcggacg tcgtatttaa tgatgacatc
      421 gccatgcgca cccgtttttc gatcagctac acgtacataa ggtgctttga cttttgtatg
      481 gtcaagatta aaactttcga cattcattct catttgtaag actcctatct gtttgttcat
      541 tcgttttgat gcttttcact aaagaattat ttagtgaaat ttacaaaaac gtctttctac
      601 ttgattctaa cacttatcgt ttccaaggtg tagcaatctg aaatattttt tagatttttc
      661 tgctaagtcg tttattggga cttctagttc ttgattctgt caaatgcctg agctaaatcg
      721 tttatgagat cctgaccata ctcaattcct acagaaagac ggatcaagcc gtcctttatg
      781 ccagcctctt ctcgtttttc ttttggaata gacgcatgag tcatgacaga aggaatttcc
      841 actaaacttt ctacgccacc taaactttct gctaaagtaa atagttggag tgattcaaca
      901 aatggaatag cagattcttc attttttaaa gtaaaagaaa tcattccgct aaagccgctc
      961 atctgctgct tagcaactcc atggagttca tgatcgggta atcctggata gtagaccttt
     1021 tctactgctg gatgagaaaa tagaaaatca gctaccacaa aagcattttt ttgatgttcc
     1081 tccatacgaa cacttagtgt cttgattccg cgctgaagta accagctatc ttggggacca
     1141 agtacggctc cgatagcatt ttggtaaaat ccgatctgat cggctagatc tttatggtta
     1201 gaagtcacta aaccagctac tacatcgctg tgtccgccta gatatttggt accgctatgg
     1261 acaacgatat cagaaccgag atctaaaggt ctttgaaaat aaggagtagc aaatgtattg
     1321 tcagcaataa ctatcaatcc atgttgatga gccagagtgg cacttttttc taaatcagta
     1381 atctttagaa gcggattgct aggtgtttca agataaagtg cttttgtatt gggtttgatg
     1441 gattgttcta ttttatcaag attactcgta tctatgatcg tgtactcgag accattttta
     1501 gtaagaactt tgtcaaatag acggaatgtt cctccataga catcatctcc taataagata
     1561 tgatcgccag cttgaaaaag agaaaaaacg gcatgaatcc cagaaagacc agaagaaaaa
     1621 gcgaatcctc gaacaccgcc ttccaaatca gcaatcagtt cttccaatgc aaaacgagta
     1681 ggattaccag atcttgaata ttcatattgt ttaggctggc cgacgccatc ttgccgataa
     1741 gtggatgttt gataaatagg tacactgaca gctcctgttg tgggatcttc actgatgcct
     1801 ccgtggatca attttgtttg aatgtgcatg tttctttcct cctataagta aatgtttttt
     1861 gataaatagc ggtctgctgc gtctggaaaa atcgtaacga cacgatgacc aggtggtaaa
     1921 cgacgaattt ctcttaatgc tgctgcaaaa gccgcaccac tcgaacttcc aactaatagt
     1981 ccttgttctc gtgctaactg cctagtatag ttaaaccctt ctacatcaga gattgtctcg
     2041 atctgattga ttgaaagggg agaaagaaac ggcggaatga attctacacc gataccctca
     2101 atttcgtgag gggctggatc accaccattt aacacagagc cctctggttc tacaccgatt
     2161 actcgaatgt cgggatattt ttctttcaaa aaggtagaag ttccagcaaa ggttccacca
     2221 ctcccaattc ctgctacaaa actatggata ttttctttga tttcatgaaa gatttctggc
     2281 cctaacgtat gatagtaagc agcaggatta tctttatttt caaattgaag aggcagatag
     2341 ctgtttgata tctcctctgc aagtttttta cttttgctga tggcaccgat gattccttgc
     2401 tcactaggag aatggatgat ctttgctccc aatgctttca tcaattgttg tttttctaag
     2461 ctaaacttct ctggaacaac aaaaaccgtt cttaatttgt atgtgagtgc ggccagagcg
     2521 atcccaattc ctgtatttcc ggcagttggt tcgatgatcg tcgttttttc attgattttt
     2581 ccttgggcaa atccttcttt gatcaaataa ctgcccaatc tatctttgat acttcctcca
     2641 ggatttaaat attctagttt tgcataaata gcagaacctt taggcataga atagtgctga
     2701 ttgtcaaagc gataaagcgg tgtggaacca atagcttctg taattgtcga gataatcatg
     2761 tgggcctcct aaaaaaagat aataaaaaaa ggagctgact attaaatcag ctccttgaaa
     2821 gacatagaga aaaattgagt agataaaaag ataagttacc tggtactcat tctatgaagg
     2881 gcaattaata gacatgacaa atagtatgct gcttttatgc accatacaac agcaacgatt
     2941 caattgtttt tggttgctga ctgtcatgtg tatccctcca agtaaaattt ttattaacga
     3001 tagcatgaaa aaaaataaaa gtcaaaggct tgaaagaaaa aaatatttcg atagcatcgt
     3061 ttacccgtgt ttatttttct gacaaatagg catgagacct taaaagatag agaatactct
     3121 agttttctgg ccggttgtct gatacaataa agttgattat gataaagaaa agaaggagca
     3181 aaaataatga tctcagcaag tgatttaaga gccggtatga ctttcgtaca agacggcaaa
     3241 ctgattaaag ttctggatgc aagccaccat aaacctggta aaggaaatac agttatgcgt
     3301 atgaaactga aagatgtccg ttcaggtgca acatacgaca caacgttcag accagaagaa
     3361 aaatttgaaa aagcacatat cgacacaaaa cctgtacaat atttgtacac gatggatgat
     3421 acagctttct tcatggattt ggaaacatat gagcaatatg aaattcctgt agaaacagta
     3481 gaagaagaaa tgaaatatat cctagaaaac atggaagtaa aaatccaatt tttcggtagc
     3541 gaagtcatcg gcgttcaatt accaacaact gtcgtattac gtgttgtgga aacacaacct
     3601 tccatcaaag gtgcaacagt cactggttca ggtaagccag cgacaatgga aactggctta
     3661 gtagtcaatg ttcctgactt catcgaaaca gatgaattgt tggaaatcaa tacagcagaa
     3721 ggttcttatg taaaacgtgc gtcaaaata
//
LOCUS       DADXTG010000175         3666 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.178, whole genome shotgun sequence.
ACCESSION   DADXTG010000175 DADXTG010000000
VERSION     DADXTG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.178"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..>3666
                     /gene="esp"
                     /locus_tag="IWI57_002545"
     CDS             119..>3666
                     /gene="esp"
                     /locus_tag="IWI57_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBC2450643.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVL
                     KAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVTFTAGEG
                     TSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATK
                     LDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKK
                     GTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1374 a          586 c          774 g          932 t
ORIGIN      
        1 tctctaagta aattagttaa cataaggtta gtaagattcc tattctgtga ataatacgta
       61 tcagatttat cttttattct tactaggaaa gtaattatta agggagttga tttgaataat
      121 ggttagcaag aataataaga gagtatttct agaaaaaaca aagaaacgag tattgaagta
      181 cagtatcaaa aaattaagtg ttggggttgc atctgttttg attggtgttg gtcttgtctt
      241 gggaacaact gaactagtta aagcacaaga tgaaatttct ccaagcagcc cacttgaaac
      301 agcgataagc tccgtacaag taggtgacaa agtagcttcg gggaatactt ttcaggagaa
      361 tcctggttat acgaaaaatt ataacttttc ggatttacaa tttagtcctc aagaactaac
      421 tggagacaca ctaaaaggca atacaattgg atttgaagtt tacggaaaac ataatattgc
      481 agcatcaact aaaaactggg aaatccgtct tcaattagat gaacgattag ctaaatatgt
      541 agaaaaaatt caggtcgatc caaagaaagg aattggatct tctagacgga cctttgtaag
      601 aattaatgat tcgctgggta gacctacaaa tatttggaag gtcaattata ttcgtgcaag
      661 tgatggatta tttgctgggg cagaaacaac cgatacgcaa accgctccta atggggtaat
      721 tacgtttgaa aagagcctag atgaaatttt taaagagata ggcatagaca atctcaagac
      781 cgatcgttta atgtatcgaa tctacttggt aagtcatcaa gacgatgata aaattgtacc
      841 gggtattgat agtacgggct actttttaac agattcagat gatttctata atagcttaga
      901 tgtatctgaa aataatcctg accagtttaa acatggatca gtaagtgcaa aatatgagaa
      961 acctaatact caaacaaaag atggttcagg atccacaggt gctaatggag ctataatctt
     1021 agatcataag ttaacgaaaa attataattt ctcatattca tcttccgcaa agggcacgcc
     1081 ttggtatgct aattacaaaa ttgatgaacg attagttcca tatgtagcgg gtatacagat
     1141 gcacatggtt caggctgaca aagtaacata tgatgtttcg tttgaatccg gaacaaaagt
     1201 agctgattta gcaatcgagc gacgggaagg tcatgaaaat tatggcatag gttcaataac
     1261 taataatgat ttaactaaac ttatcgattt tgctaatgca agtccacgtc cagtcgttat
     1321 tcgatatgtt ttacaattga ctaagccatt agatgaaatc ttagaagata tgaaagcaac
     1381 agcacaagtt gaagaaaata aaccatttgg tgaagatttc atctttgatt cttggttgtc
     1441 ggatacgaat aaaaaattaa tccagaacac ttatggaaca ggttattatt atttgcaaga
     1501 tattgatggt gatggaaacc ctgacgataa agaagagagc ggagacacga atccatatat
     1561 cgggaaacct gaattagaag aagtatatga tgttgacaca acagttaagg ggaaagtatt
     1621 catccacgag ttagcgggaa caggtcacaa agcccaactt gttgataaag aaggtactgt
     1681 attagcagaa aaaactatcg ctccaaatga aaaagatggg gctccaattt cagatactgt
     1741 agaatttgaa tttacgggtg tagattcaag taaattaatc gcgaaagatg aattaaaaat
     1801 ccaaatcgtt tctccaggtt ttgataaacc agaagaaggt tcaaccgtta ttaaggaatc
     1861 accaaaagcg gttgataaac aaaccgtggt agttggattt aaaccagatg ctaaagaatc
     1921 aattcggaat aataaaaact tacctgaaga tgcagagtat tcatggaaaa cagagcctga
     1981 tacttctaac gttactgata gtacgaaagg tattgtaact gttaagatcg gaaatcgaac
     2041 tttcgacgtg gatgtagagt ttgctgtaaa agcttctcaa gctatggaaa atgatgcaac
     2101 atacgtacct ataacaacaa ctccagaaac gacagttcaa agtggaaaac ctacatttga
     2161 taaaccagat gttcctttag ctaaagatgc cttttcaatt tcagatgtct ataataagga
     2221 cttcggtaat gcaagtgttg atgcaaacac tggtattgta acattcactc cagctaaagg
     2281 tgtaggagaa tcggagccga ttactggaac aattcctatt aaaattgttt accaagatgg
     2341 ttctgtaggc acgaccgatt tagcagtaac tgtaagtaaa gatatttatg aaaatccagg
     2401 agaaaacatt cctgcaggct accacaaagt aaccttcacc gcaggggaag gaacaagtat
     2461 tgaaagtgga acaacagtct ttgcagtgaa agacggcgta agcttgccag aagataaact
     2521 tccagtgttg aaagcaaaag atggttatac agatgcgaaa tggccagaag aagcaactca
     2581 accaattaca gcagatgata cagaatttgt atcaagtgca acaaaactgg atgatatcat
     2641 tgaaaatcca ggagaaaaca ttcctgcagg ctaccacaaa gtaaccttca ccgcaggaga
     2701 aggaacaagt attgaaagtg gaacaacagt ctttgcagtg aaagacggcg taagcttgcc
     2761 agaagataaa cttccagtgt tgaaagcaaa agatggttat acagatgcga aatggccaga
     2821 agaagcaact caaccaatta cagcagatga tacagaattt gtatcaagtg caacaaaatt
     2881 ggatgatatc attgaaaacc caggggataa cattccggct ggctatcata aagtaacctt
     2941 tactgcagga gaaggaacaa gtattgaaag tggaacaaca gtctttgcag tgaaagacgg
     3001 cgtaagctta ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc
     3061 gaaatggcca gaagaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag
     3121 tgcaacaaaa ctggatgata tcattgaaaa tccaggagaa aacattcctg caggctacca
     3181 caaagtaacc ttcaccgcag gagaaggaac aagtattgaa agtggaacaa cagtctttgc
     3241 agtgaaagac ggcgtaagct taccagaaga taaacttcca gtgttgaaag caaaagatgg
     3301 ttatacagat gcgaaatggc cagaggaagc aactcaacca attacagcag atgatacaga
     3361 atttgtatca agtgcaacaa aactggatga taaatctgac gctgacaaat ataatcctga
     3421 aggtcaaaaa gtgactacag aattgaataa ggaaccagac gcatttgagg gaattaagaa
     3481 caaagaagat ttaccaaaag atactaagta tacttggaaa gaaaaagtag atgttagcgc
     3541 agctggaaat aaaaaaggga ctgttgtagt gacatatcca gatggatcat ctgatgaagt
     3601 tgaagtagat gttacagtga cagacaatcg ttctgacgcc gataaatatg agccaacagt
     3661 agaagg
//
LOCUS       DADXTG010000176         3537 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.179, whole genome shotgun sequence.
ACCESSION   DADXTG010000176 DADXTG010000000
VERSION     DADXTG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.179"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            61..1335
                     /locus_tag="IWI57_002546"
     CDS             61..1335
                     /locus_tag="IWI57_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2450644.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1389..2816
                     /locus_tag="IWI57_002547"
     CDS             1389..2816
                     /locus_tag="IWI57_002547"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBC2450645.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2829..>3537
                     /locus_tag="IWI57_002548"
     CDS             2829..>3537
                     /locus_tag="IWI57_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008841195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBC2450646.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVV"
BASE COUNT         1160 a          491 c          723 g         1163 t
ORIGIN      
        1 taaaaatatt aaccggttat catgaaaggg gatacaaata tatcgttgga ggataaaaat
       61 atgtcaaagg ttaacgtatt taaaaatcaa tcctatttac aaagttcagc tacattatta
      121 ctattttttg cttcttgggg tgtttggtgg tcattttttc aactttggct aacatctgaa
      181 tcaaatggtt tagggttatc tggcagtgct gtaggaacag tattctcggc aaattcgtta
      241 gttaccttaa ttttgatgtt tatttatgga acattacaag ataaattgta tattaaacga
      301 aatttattaa tttttgcttc tgtattagcg acacttgttg gaccattttt tatatggata
      361 tacgggccat tgctagataa caattttaat ttaggcatta ttatgggagc actatttttg
      421 tcagctggat atttagcttc tgtaggagtt tttgaagctg tgtcagaaag gtttagtcgt
      481 ttatttggct ttgaatatgg acaagcaagg gcgtggggat catttggtta tgccttggta
      541 gcgcttttgg caggattttt atttgtaaaa aatcctcatt taaacttttg ggcgggatct
      601 ttctttggtt ctttactatt gttaaattta ttattttgga accctaaagt tgaacgggaa
      661 gcaaatcaaa attttaatca agaacaagct gaatcaaata gtattccttc tttaaaagaa
      721 atgtttgatc taatgaaact gcctcaatta tggacgataa tcatctttat tgtttttaca
      781 tggacatttt atacggtatt cgatcaacaa atgtttccgg gattttatac tggtttgttt
      841 tcaacatcag ctaatggtga aaaaatatat gggacattga atgctattca agtattttgt
      901 gaagcgttaa tgatgggaat tgttccaatc attatgagaa aattaggggt tcgaaatact
      961 ttgttattag gtgtaaccat tatgtgtgta cgaattggat tgtgcgggtt tgcctcgaca
     1021 ccattatctg tttcatgcat aaaaatgttg catgctttag aagtaccatt atttacatta
     1081 ccaatgtttc gctattttac acttcatttt gatacaaagc tatcagcaac cctctatatg
     1141 ataggatttc agatagctgc tcaaattggg caagtgattt tatcaacacc attgggaata
     1201 ttaagagaca acgttggcta tcaaccaaca tttaaaatta tttctcttat tgtattacta
     1261 gcaggcatat atgcattctt tattcttaaa caagatgata gagatgttca aggggatcca
     1321 tttattcgag gataatggaa accaacagaa taaaaaacaa ttttatctag aaagtgagcg
     1381 aatatattat gacaaaaaga gctatagaat taacaaatac acgttatcgt ttgggatatc
     1441 atgtgtctgc accatcagga tggattaatg atccaaatgg attttgctat tttgatgggt
     1501 attatcatgt gttttatcaa catcatccat atagtgctga atggggacca atgcactggg
     1561 cacatgctag aagtaaggat ttagttcatt gggaatcttt accacttgct ttgactcctg
     1621 gagatcaaga agatgaaggt ggttgttttt caggaagcgc tattgaaaaa aatggtgtgt
     1681 tgtatctgtt ctatacagga catcactatt atggagacaa tgatccagat catttttggc
     1741 aaaatcaaaa catggcgtat agtacggatg gcattcactt tactaaatat gaaaaaaatc
     1801 ctgtaatagc aaaagcacca gaagataaca cacatcattt cagagatcca aaagtatgga
     1861 aacacgatga aaaatattac atggttttgg gtagtcaaga aaaagatgga ttaggtcgtg
     1921 ctattgttta tcagtcgtta gatttattaa attgggatta cattggacca atttctaaag
     1981 cgaaggaagt acttacagaa ggatttatgt gggaatgccc agactttttt gaattaaatg
     2041 gaaaatctat tcttttatta tcccctcagg gaattgaagc aaatggaaaa gattatttaa
     2101 atctatatga aacaggctac tttatcggtg attatgatta tgagacagca aaattcatta
     2161 gaggaaattt ttatgaatta gataagggac acgatttcta tgcaacacaa acaactttat
     2221 ctccagatgg tagacgtatt gttatggctt ggatggatat gtgggaaagc cctatgcctg
     2281 aaaaagtaga cggatgggca ggagcattaa cgcttcctcg agaattagag ttaattggag
     2341 atcatttgta tatgaaacct gtgagagaat tagaacaact tcgagttggc agcggcgttg
     2401 aaacaaaagt taatttggca ggagaaataa taatcgctca agatgcttca ttcaaagaag
     2461 ctctaattga tgtactattg actaaatcaa aaaatcaaga aatcaaattt tcattaaaaa
     2521 caaaagatga agagttaatt aatatttttt attctaagga taaaaatgaa tgtaccttaa
     2581 ctcgtaacgg caaaaatgat ccaaggtatg gaaccattca gatgtgtgat agattatcac
     2641 ttcgagtgtt tatcgataaa agttcggcag agatttttat taatgatggc gagcttgttt
     2701 ttacagagcg ttattataca gaaaatcaac caagtatgca tttgagtgtt agtgaagaag
     2761 cttttgtctc gagtacaatt tatcatctaa aaaaagatgc aatttcttac gagtaaggag
     2821 gttttatgat gttaattggt agcatagaag caggtgggac taaatttgta tgtgccgtgg
     2881 gagacgagga ttaccgagta caagaccaaa taatttgtcc aacgaccacc cccagggaaa
     2941 cattgaaaaa aactgttgac tattttaaac agtttgatat aaatgcatta ggtattgcat
     3001 catttggtcc aatagaaatt cgaaaaaatt caccaaaatc aggatatatt acgtctacac
     3061 caaaggtggg atggcgaaat gttaatgtgt taggttattt acaagatgag ctgaatgttc
     3121 caatgtcttg gacaacggat gtgaacggat cagcttatgg agagtacatc atgtccactt
     3181 tatcgaatga aaaaataaat tcattagttt attacaccgt tggtactggc gttggtggtg
     3241 gagctgttgt tgacggaaaa ctattaggat cgtttggtca ttcagaaatg ggccatacgt
     3301 ttgtaaaacg ccatcctgat gatattgatt ttaaaggaat ttgtccttat catggtgatt
     3361 gtttagaagg cttagttgcg ggaccaacgt ttgaagcacg ttttggaaaa aagggtgaag
     3421 acgtttcttt aacggatcct atttgggatg taatggccta ttatctagca caagctgtga
     3481 ttcaaacaac attgattata cgaccggata aaatagtatt tggtggaagc gtagtaa
//
LOCUS       DADXTG010000177         3525 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.180, whole genome shotgun sequence.
ACCESSION   DADXTG010000177 DADXTG010000000
VERSION     DADXTG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.180"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..325
                     /locus_tag="IWI57_002549"
     CDS             <1..325
                     /locus_tag="IWI57_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450647.1"
                     /translation="ELKVSSKSILNYLTDLEELFQKYPDKVILKNENNRRFSMEKQEN
                     FPIYIIYLHFYRKSYNYHLIEFMYQHPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEM
                     TDVKE"
     gene            complement(491..1684)
                     /locus_tag="IWI57_002550"
     CDS             complement(491..1684)
                     /locus_tag="IWI57_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2450648.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            1908..2285
                     /locus_tag="IWI57_002551"
     CDS             1908..2285
                     /locus_tag="IWI57_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2450649.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2273..2500
                     /locus_tag="IWI57_002552"
                     /pseudo
     CDS             2273..2500
                     /locus_tag="IWI57_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2592..>3525
                     /locus_tag="IWI57_002553"
     CDS             2592..>3525
                     /locus_tag="IWI57_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2450650.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVT"
BASE COUNT         1283 a          679 c          646 g          917 t
ORIGIN      
        1 cgaattgaag gtatcaagta agtctatctt gaattatcta acagatttag aagaattgtt
       61 ccaaaaatat cctgataagg tcattttgaa gaatgagaat aatcggcgct tttctatgga
      121 aaagcaggag aacttcccaa tctacattat ttatcttcat ttctacagaa aatcatataa
      181 ttatcatttg attgaattca tgtatcaaca tcctgagaaa aatttggaag attatgcaga
      241 cgctcaatat acgagtgtat ccacagtttt tcgttatgct aagttattag taaagtactt
      301 tgaaatgacg gacgtcaaag aatagtagcc gccagataga aggaaattag cagttgaaaa
      361 agctgccctg catcaagtaa tacgaccagc caaacggaaa cagtgtctca tgtttttgat
      421 ttggctggtc ttattaccgc agatgcaagc ttttgccaca cctctttctt gtcatttaca
      481 gactcaacta tcaatgctgc aagctttccc ttaatgctgt aacaccagta agagttgtca
      541 agaccagcag actgactagg taaataccgc tcaaaaacaa gattacggca gttaaactat
      601 aatgatccat caagaaaccg atgacgatag aagaaaaacc accaacagct cgtccaaggt
      661 tcataatgaa attattcgcc gtcgcgcgga tttctgttgg atacaaacta ctaatcactg
      721 cgccatatcc accatacatg ccattgataa agtagccgac tatcacagcg gctgctacca
      781 aactccactg gctatgggct aacacaagta gatagacaga taaggatgaa caaattaaaa
      841 acaaagaaaa ggctagttta ggccccaatt tatccatgat tatgccaaaa gtcatcatcc
      901 ccaaagacat gcctaaaata gtactgacca tccacaatga agacccagaa accgaaagcc
      961 ctaatcgatt ttgcataatt gaaggcagcc aattcattaa cccaaaatag ccggcaattt
     1021 ggacagttac catgatacta aggccacatg tttgccaagc aagatgtttt gattcaaaca
     1081 gtctgctaag tttggttttc ttttcggcag attgtacaga tttgaattcc tttggttcag
     1141 tcaagccgcg acgaatgaac aagaccatca acaccggcag tacacctacg acatacaaca
     1201 tcttccaacc aaacgttgga atgatcacag cagctaaaat cgcagccaaa gccgcaccga
     1261 cctgcgcacc aataccagca accgaagaag ctcgtccaat ttgttttttt gaaaaacttt
     1321 cggaaatcaa ggacatacac gccccgtatt ctcctcccgc tcctacacct gcgataaagc
     1381 gaaagagata aaccaagtag atgtttgaag caaaatacat caataaagaa gcaatcgaaa
     1441 acagaaggac cgtttgagaa aatactttca cacgtccata tttatcagct atgatgccaa
     1501 aaaaaatacc tccaatgagc atccccaaat tagtgatcgt tgaaatgaaa cccgcttgcg
     1561 taccactaat atgaaaagaa gtaatcattg aagataatga aaaagctaaa aacataatat
     1621 ccatattctc taaagcaatt cccgccgatg tcgataataa taactttcgc tgatacgttg
     1681 tcatttttat accccttttc ctaaaatttc cctaaattgt taaaagtata tccaaaaaag
     1741 aataaaaaaa caagactttt ttatttgaaa taatacacaa acaaccgata tagataatcc
     1801 agcttagttt taatagaaat gagtattgac ttggagtaca cttaaagttg tagaataaat
     1861 aaaatacata agtaaagtgg caagaaatta agagaaagag tgatcagatg aatatcaaac
     1921 aagtcagtga agaaaagggc atttcggccg atacattgcg ctattatgaa cgaatcgggt
     1981 tgattccacc agttaatcgg acaaaaggcg gaatccgtga ttatacagaa gaagacctaa
     2041 aatgggtcga ctttacgatc tgtatgcgaa atgccggatt atcgatcgag tctttgattg
     2101 aatatattcg cctatctagc gaaggagaag caactgtatt tgagcggcgt aagcttttga
     2161 ttgaagaaag tgaacttttg acgaaaaaaa ttgctgaaat gcaagaatgt ttggaacgtt
     2221 tacaaggaaa aatcaagaaa tatgatcgtg tgttatctag aaataaaatg gaatgtatca
     2281 agtaaaaaaa tcaaggagca tcgctatgga gttacgctta ctaaaatatt tttggacaat
     2341 tgcagaagaa ggaacaatct ctcaagccgc agaagttcta catcttacac aaccaacctt
     2401 aagccgacaa ctgaaggagt tagaagagga actaggaaca gaactgtttg tacgcgaaaa
     2461 ccgcaaaatg atcttgacag aagcaggtta ttttctacgc cgattgtaaa attaagctag
     2521 acaactaaaa aaagtcattt gtgctacact caaaatagtt ccgacgaaag aattataagg
     2581 agtgagtaca aatgacctat aaccatctta caccaactga actcgtaatg atagaagctt
     2641 atttcaatca atcacaacct gtttcaaaag tagccaatct gttacaacgt tctcgacaaa
     2701 ctatttataa ggtctatcgt tttcttaaat ctggtggaag cgcaatgagc tactataaac
     2761 aatataagaa aaacaaacgt aactgcggaa gacatccgat tgttttacct gatgagcaac
     2821 aggaatacat tcaaaaaaag gttgttcaag ggtgggctcc tgatgtcctt attggtcgag
     2881 cagagtttcc tattgcttgt tcggtacgca cactgtatcg aatgtttaaa gataaaacct
     2941 ttgacactca cgatttacca atgaagggaa aaagaaaacc aaacggtcat aaagaaaaaa
     3001 gaggaaaaca agggtttcgc cggtcaatcc gagacagaaa gacagactat acccagtttg
     3061 atcatgaatt tggtcacctg gaaggtgaca ctatcgtggg tggaaaacat aaaagcgcgg
     3121 tcatcacatt agttgaacgg ctatctaaag tcatcattac cttgaaaccc gaaggcagac
     3181 aggctaaaga cattgaaaat aggctgaatc aatggtttcg ttgtgtccca aaaaacttat
     3241 ttaaatccat cacatttgat tgtggaaaag aattctcaaa ttggaaaaag attagtaata
     3301 ccaacgatat ttcaatttat tttgcggatc caggaacacc ttctcaacga ggattgaatg
     3361 aacattcaaa tggcttatta cgtaaagatg gtttgtataa agctatggat tttaatggtg
     3421 tatctgaaac ctttatccaa tcggtggcat cgaaacgaaa tcatattcct agaaaatcat
     3481 tacactatcg gacaccaatg gaagtatttt tgagttacgt cacaa
//
LOCUS       DADXTG010000178         3512 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.181, whole genome shotgun sequence.
ACCESSION   DADXTG010000178 DADXTG010000000
VERSION     DADXTG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.181"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..78
                     /locus_tag="IWI57_002554"
     CDS             1..78
                     /locus_tag="IWI57_002554"
                     /inference="COORDINATES: protein motif:HMM:NF021182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450651.1"
                     /translation="MYRDTERFLTVLGNVYENPELLEVE"
     gene            79..540
                     /locus_tag="IWI57_002555"
     CDS             79..540
                     /locus_tag="IWI57_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2450652.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            566..1018
                     /locus_tag="IWI57_002556"
     CDS             566..1018
                     /locus_tag="IWI57_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450653.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            1124..1318
                     /locus_tag="IWI57_002557"
     CDS             1124..1318
                     /locus_tag="IWI57_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450654.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            1349..1642
                     /locus_tag="IWI57_002558"
     CDS             1349..1642
                     /locus_tag="IWI57_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450655.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            1672..2112
                     /locus_tag="IWI57_002559"
     CDS             1672..2112
                     /locus_tag="IWI57_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2450656.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            2187..2654
                     /locus_tag="IWI57_002560"
     CDS             2187..2654
                     /locus_tag="IWI57_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBC2450657.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            2949..3086
                     /locus_tag="IWI57_002561"
     CDS             2949..3086
                     /locus_tag="IWI57_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450658.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQLQEALDEIANFKIQV
                     S"
     gene            complement(3067..3381)
                     /locus_tag="IWI57_002562"
     CDS             complement(3067..3381)
                     /locus_tag="IWI57_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450659.1"
                     /translation="MSNGFDDLSKKLNKMAKAAQEMDGEHSVPMSELFTNDFVSANTK
                     FSSIEEFFEVSGFDCSSEESFAAIPDDQMDAFVSDNTNFDTWSDFMSAAAIKYTAKKL
                     GF"
BASE COUNT         1221 a          562 c          727 g         1002 t
ORIGIN      
        1 atttatcggg atacagaaag atttctcact gttttaggta acgtctacga gaatccagag
       61 ttattggagg tagagtaatt gccaaaaata ttagatgcat gttgcggtag tagattgttc
      121 tggtttgata agaacaattc tgacgtaaca tttatggatt gtcgtcagca gtatgaagaa
      181 ttatcaacag gtcatgtgat caatgttgat ccagatgtgg tcgcagactt tcgcgatatg
      241 ccttttgaag atgatgtgtt cgatatggtt gtatttgacc cgccacactt aagacatgtc
      301 ggcgaaaact catggcttgc caaaaaatat ggaaaattag acgaactttg gccagaagac
      361 atcaggcaag gttttgcaga gtgtatgcga gttttacgac cttcaggaac attaattttt
      421 aagtggaacg aggaacagat accactttct gatgtgttag aggctattgg agaacagcca
      481 ttgtttggta ataaacgaag caaaacacat tggcttgtgt ttatgaaaaa attcagctaa
      541 cggaggaaac aaaggaggaa aagtaatgaa gattgtatta aataaatgtt ttggtggctt
      601 tgggttgagc catgaagcaa aaatggaaat ttttaaaaga aaaaatattg aagtattccc
      661 atacatcaat aactttagct atgactcaga tgatgaatac acgagataca caggccaaaa
      721 gctaggttcg atggatttta tctactattt caaaaaagac ccgaaaatcg acaaagttac
      781 aggaatttat agcgaaattg accgactgta cggaattgct gatgattcca gtttttcaag
      841 tgattcaaac agaggtgata aggacctcgt tgctgttgtc gaaaaactag gaagtgaggc
      901 cagtggacca tatgcaagtc ttaaagtcgt tgatataccc gatggggctg agtgggaaat
      961 aagtgattat gacggcgttg aaactgcaca ttatggtttt caaacaggta gtatttagtc
     1021 cactaatgac aactaaagag gaggcagaag gatgagtgaa gatgaatact ttgaacagta
     1081 gtctattaac gacagagatt tttaactgga aaggggtgta gtcatggacg catcggaagt
     1141 agtaaataaa attaatagtc tcaaagaggt tttcggagac gtcgaagtag tgataaacgc
     1201 acaaggctat gacaacgaat tttttaagca gattaccgac attagcattc agcaaggaat
     1261 ggaagacgaa gacggaaact ttatcgatga agtagccatt ttagtgactt gcgagtaagt
     1321 aattgacagc taaagaggag gaagcggaat gaaactaaaa gacggatttt actccagcgg
     1381 ccacggcatc ggcggtttaa tgctagatat gccgacaaag aatcctaaaa cacgtaagaa
     1441 accaaaattc aaagtcggtg acatggttcg ctgcgaagca gaaggattca tctatccatt
     1501 tcgtggatat gtagaacacg tctataatca ctcagcaatc attcgcattg aaaacacgat
     1561 ggaatgtgat aagtggttag cgaaaagcaa agagaattta gcagtggtga gattggtgga
     1621 catggaagtt ataaaatctt aattttgctt ttttacaact atcctacaac aatgagcgtt
     1681 attccacaac tggaaggaga agaggcaatt gtgaacattt tagagaatat agatattaag
     1741 caaacaagga aaaatgctag acgattacta aaaagataca gaaatttaga acgtttggtc
     1801 ggaccagtga aaatagactt ttccgtgatg actgttacca aaaatttgaa attcacaatt
     1861 gacagtcaaa acgaagaaat cattgaagcg ataagtactc gagattcggt tatcgaagca
     1921 ttaacacggc taagtagaat ccatttccag gtactttatt atagttattg ttttcctaat
     1981 aagatgtcaa tgtatcaaat aggagaaaaa ttaggatatt ccgatagaac tatcgagaga
     2041 atgaaggcag tagctttggt tgaatttgct gaggcgtata aatcaggaga actcatttca
     2101 cgtacaaaat aaaaaaagtc ggattcctcc gactatgaat aatatttccg acataagtat
     2161 tatatcataa ttgggggaat cagaggatgg tacttttcga tgtaaagaaa tatgagacac
     2221 caagcgcgaa agatgttgat atggaacgca caaaacataa tgtcgctgtg tttctttcag
     2281 catatctatc agctagatgt agagtagggc aacctcgaga accaaaagtg acagcatctt
     2341 attccttggt tccaccttct acagctgatc atatatttga agcagaaaga atgctgatcg
     2401 ataaagaaga agcacaggaa gaatttgagt atctgcataa attgtttgtt cgaggctatt
     2461 cagcgataca gcatccgcat aagcctgatg tgactgaaag gcgcaagaag atattctatg
     2521 atcgttatat caatggtctg cccatttatg taactgctca aaggaataat actagcgaag
     2581 aatcggttaa agtagaatca aacagaatta tcatccaatt tgcttcatcg ttagaactgg
     2641 ttgctttcaa gtagccagtt tttacacttt ttataccctt ttattaccaa tttggtttcc
     2701 attttatacc ttttttatac caatcactta cctattcaac gttgtattat gatagtgtcg
     2761 aaagattagg aaacaggatc gacaaaataa aatgtaaggg aggaaatctc cctcatcgtt
     2821 taattaagct tcgataggca gcaacggaaa tattaagaat aaggatgtga atttcaactc
     2881 cttctaaatt gttcttatta tctatcatcc gttgctgtct attaatttat gtattggagg
     2941 aaaaacgaat ggataaagaa atcaaagcaa ctgtcaaatt agatttgact gaactaaaag
     3001 aactgctcaa caaggctagt gaccaagtcg aacagttaca agaagcttta gatgaaattg
     3061 ctaattttaa aatccaagtt tcttagcagt atattttata gcagctgcac tcatgaaatc
     3121 agaccaagta tcaaaatttg tattatcaga tacgaatgca tccatttgat catcaggaat
     3181 cgcggcaaac gattcttcag agctgcagtc aaatccgcta acctcaaaga attcctcaat
     3241 ggagctgaat ttggtattag ctgaaacaaa atcatttgta aataactcgg acattggaac
     3301 gctatgttca ccgtccattt cttgggcagc ttttgccatt ttgttaagtt ttttacttaa
     3361 atcatcaaac ccgttactca tgttatcacc acctataatt tatttcagcg gaccactcgc
     3421 tgataaataa aattatacgc ttagtattta ttttcacaat attaatttat cgtaataact
     3481 tttgagtaaa tagaacaaaa aacctgcact ag
//
LOCUS       DADXTG010000179         3506 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.182, whole genome shotgun sequence.
ACCESSION   DADXTG010000179 DADXTG010000000
VERSION     DADXTG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.182"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(52..1518)
                     /locus_tag="IWI57_002563"
     CDS             complement(52..1518)
                     /locus_tag="IWI57_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBC2450660.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1905..2501
                     /locus_tag="IWI57_002564"
     CDS             1905..2501
                     /locus_tag="IWI57_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBC2450661.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2559..3041)
                     /locus_tag="IWI57_002565"
     CDS             complement(2559..3041)
                     /locus_tag="IWI57_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450662.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3147..3344)
                     /locus_tag="IWI57_002566"
     CDS             complement(3147..3344)
                     /locus_tag="IWI57_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450663.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1188 a          704 c          578 g         1036 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
     1681 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
     1741 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
     1801 ttttttgtga gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat
     1861 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
     1921 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
     1981 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
     2041 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
     2101 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
     2161 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
     2221 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
     2281 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
     2341 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
     2401 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct ggggaaaaag
     2461 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
     2521 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
     2581 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     2641 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     2701 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     2761 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     2821 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     2881 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     2941 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     3001 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     3061 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     3121 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     3181 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     3241 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     3301 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     3361 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     3421 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     3481 ttaaaacagc tgttgtattt agaaga
//
LOCUS       DADXTG010000180         3451 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.183, whole genome shotgun sequence.
ACCESSION   DADXTG010000180 DADXTG010000000
VERSION     DADXTG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.183"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            160..2325
                     /locus_tag="IWI57_002567"
     CDS             160..2325
                     /locus_tag="IWI57_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBC2450664.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2629..>3451
                     /locus_tag="IWI57_002568"
     CDS             2629..>3451
                     /locus_tag="IWI57_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBC2450665.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELD"
BASE COUNT         1116 a          552 c          819 g          964 t
ORIGIN      
        1 aaaataaatt tttaggagaa aattatagaa gggaacacac gtattatagg ttttatagaa
       61 tggtatagac agaaaaataa aaaataaagc atttacaaaa aagaaaacgc acgctataat
      121 acgtttaacg aaaccgattg cgtaaaggag ctaaacggaa tgaaaaagat atttagtttt
      181 gagttttggc aaaaatttgg aaaggcttta atggttgtgg tagcagttat gccggcagct
      241 gggttgatga tcagtatagg gaaatcaatt cccttgatca atcctgattg gacgccgctt
      301 gtcacaacgg gcggagtaat tgaaaatatt ggttgggcaa ttattggtaa tcttcatatt
      361 ttatttgctt tagcaatagg aggaagttgg gcaaaagaaa gagcaggcgg agcatttgca
      421 gctgggatct catttatttt aatcaatcgg atcactgggg ctatctttgg tgtcacaagt
      481 gacatgcttg cgaatgaaga tgcatttacc cacactctgt tcggtacgaa gatcatggtt
      541 aaaggatttt ttacaagtgt attggaagca ccagctttga atatgggagt attcgtaggg
      601 atcatcgctg gatttgtcgg ggcaatggct tataataagt actataatta tagaaaattg
      661 ccggatgctt tgtcattttt caatggaaag cgctttgttc cgtttgttgt tatcttatgg
      721 tcaacaatcg tggcattgat tcttgctgtg gtgtggccaa acgtacaggc tggaatcaat
      781 aattttggtt tatggattgc agaatcacaa gaaagcgcac cgattctagc accgttttta
      841 tttggaacat tagaacgttt actattgcca tttggattgc atcatatgct gacgattcct
      901 atcaactata cacagcttgg cggatcgtat gagattttat caggcgcaca ggcggggaca
      961 caagtatttg ggcaagatcc gttatggttg gcttgggcca ctgatttagt gaatcttaaa
     1021 ggtgcagggg atatgtcgca atatgagttt gtgttgcata actggacgcc ggctcgtttc
     1081 aaagtaggac aaatgattgg ttcttcaggt atcttgatgg gaatggcttt tgcgatgtat
     1141 cgtaatgtcg atcctgataa gaaagcaaga tataaatcaa tgtatttttc agcagcgttg
     1201 gctgtctttc taacaggtgt aacagaacca ttggagttca tgtttatgtt tgctgctgtg
     1261 ccattatatg tcatttattc agtgatccaa ggggctgcat ttgcgatggc agatatcttg
     1321 ccattgcggg tccattcatt tgggaatatc gaattgctga ctcggacacc gttagctatc
     1381 aaagcgggtc taggtggaga tcttatcaac ttcgtgttgt gtgtgatcgc ttttggtgtt
     1441 gttacgtatt tcttagcaaa tttcttgatc aaaaaattca actttgctac accgggacgt
     1501 aacggaaatt acgacaatga cagtgaagaa accgttgtat ctaacagcgg aacaggaaca
     1561 gcagatcagc aagtggtcca gatcattcat ttgctgggag gaaaagaaaa tatcaaagat
     1621 gtcgatgctt gtatgactcg gttacgcgta agtgtaaatg atcgagaaaa agtagggact
     1681 gaagaagcct ggaaacgagc aggggcgatg ggattgatcg tcaaagacaa tggagtccaa
     1741 gcagtttatg gaccaaaagc agatgtcttg aaatcagata tcgaagatct gctgcaatca
     1801 ggagcagaga ttccagagcc agaaatggtt tctacaggaa atggacaagc aggtagtaag
     1861 caatacttag gtatagaaca agaactagtt tccgcagctt ctggagaagt gattccttta
     1921 tctgaagtaa aagatccggt attctcacaa aaaatgatgg gtgacggctt tgctgttatt
     1981 ccaacatcaa gagaagttgt tgcgccgatc gctggaaaag tgacaagtat tttccctagc
     2041 aaacatgcaa ttgggctgga aacgaaagat ggtatcgaag ttttgatcca tatgggaatc
     2101 gatacagtcc aaatgaatca gccagctttt gagattgctg tcaaagaagg ccaagaagta
     2161 ggaacaggaa cgaaattagc tgagatggat cttgatgtga tccaaaacga agaaaaagat
     2221 ccaacgatca tgatcgtatt tacaaacgat aaagtagaag aagtagtgat caagcaatta
     2281 ggtacagtga cagctggtaa agtgatcggc tccattaaaa tgtaaggaga ggttgtgaaa
     2341 aagctgtcct gcatcaagtc ataagaacag ccaaacaaaa atagctcctc ctatttttcg
     2401 tttggttggg cttattaccg agaagcagca gtttgaatac tgtttagtgg agaggtcgtg
     2461 aaaaagctgc cctgcaccaa gtgataaaaa cagccaaaca aaaaatagct cctggtccat
     2521 tgagcttatt actgaggagt tagcaggaga acactatttg ctggagaggt tgtgacaaaa
     2581 attttgtcac aacccttttg tttcgaaatg aagacaagga gaaaaccaat gaaattcatg
     2641 acactcaaca cgcatagctg gcttgagcca gaaccagaga aaaaattgca agaattagca
     2701 gataagatcc tgttagaaga ttatgaagtg attgctttac aagaaatcaa ccaattactg
     2761 gaatctgaag aagtagaacc tgcgaagcta atgaagttct gtccggtaaa gaatcaagta
     2821 cctatccgta aagataattt tgcctaccgt ttagttcagc ttttaaagga acacggaaaa
     2881 gagtattttt ggagttggga aatgagccac atcggttatg ataaatatga agaaggaaat
     2941 gcactgctta cgaaaaaaac attggaaagt caggtcctta ccgtctccca atttcaaaag
     3001 aaagaagatt atcagacacg caagatcttg attgggaaaa caaagataga tgatcaggac
     3061 atttttgtct cgagctgcca cttttcttgg tggacagata aaaaaagtgg cttttatttt
     3121 gagtggaaaa acttggaaaa ctactttttg gaaactcgcg taccactttt tttcctaggt
     3181 gatttcaata atccagtaga ctctcaaggt tattatactg taagagaaag ttgccttctt
     3241 cttcaagatt cgtatgttgt cgcgaatgaa aaaggaaaag cagctactgt ggaaaagaaa
     3301 atagatggtt gggaacaaaa tacagaaaaa ttgcgtatag attttatttt cgtccctgaa
     3361 ggaatgcagg ttaaaaaata tcaacggatt tttgatggga tcgatagtcc aatcatcagt
     3421 gatcactacg gggtggaaat cgaattagat g
//
LOCUS       DADXTG010000181         3390 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.184, whole genome shotgun sequence.
ACCESSION   DADXTG010000181 DADXTG010000000
VERSION     DADXTG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.184"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..622
                     /locus_tag="IWI57_002569"
     CDS             <1..622
                     /locus_tag="IWI57_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HBC2450666.1"
                     /translation="DMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEAL
                     LSVSVPRELFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYEL
                     AKLFHSVDGKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSD
                     LKKIELIEALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
     gene            710..1108
                     /locus_tag="IWI57_002570"
     CDS             710..1108
                     /locus_tag="IWI57_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HBC2450667.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            1133..1849
                     /locus_tag="IWI57_002571"
     CDS             1133..1849
                     /locus_tag="IWI57_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2450668.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
BASE COUNT         1047 a          440 c          685 g         1218 t
ORIGIN      
        1 agatatgtat ctcgataaaa catttcagag attcaataag ctcagaaagg acccccggtt
       61 cacagaattc ttttcttctg atattcaaat aaatggagtc agatataaat ctttggacca
      121 gatagaagct ttattgagcg taagtgttcc aagagaattg tttgacatta cgcaatttaa
      181 tattattcat ggtgacttat gctttgccaa tattatggta gataacacat tttcgttcat
      241 taaagtcata gatccgcgag gtaagtttgg agattttgat atttatggtg attatagata
      301 tgaactggca aaattgtttc atagtgttga tggaaaatat gactttataa ttaaggactt
      361 gtttacaata aaatatgatc caaagaaggc aatcatagac tatattgttc aagatcgtaa
      421 acgagattat gatttatatg aagtatttta ctctgtcttc aaagatgaaa taggttctga
      481 tttgaaaaaa attgaactta ttgaggcttt gctgttttta agtatgattc ctttgcatgg
      541 agaaagctta aaccatcaga tggctatgtt agcaactggt ttggaaattt taggacgagt
      601 tgtcccggat atttattgtt aatgctttga gctattgttt ttctatttga tcagtgtaaa
      661 gccgggcata taaattgcct ggctaaaata aaggagatgt cttagttata tgtttaaaga
      721 ttacgcattt gtttttgata ttgatggaac tttgtgcccc atcaaaaaga aagatgagaa
      781 atatgaagat ttagttccgt atgctaatat ggtggagaag ttgcgatact ataaggagaa
      841 cggagcaaag attacactgt tcacatctcg caatatgaat tcttatcatg gtaatttagg
      901 tgtgatcaat gcaaatactg caaaaatctt gttggcttgg ctagataagt gggagattcc
      961 gtatgatgaa attctttatg gaaagccttg gccaggccat aaagggtttt atgttgatga
     1021 tcgcacagtt cgtccggatg aatttttatc gtgtaccctg gatcagctcg atgagatttg
     1081 caaaaaatcc aaattgaaat ctgagtaaag gagactttat aaagtgaata agatgacggt
     1141 tattattaca atggctggtc ttggcagtcg ctttcgtaaa gttggctaca ctgtgcccaa
     1201 atttatgatt gaagcaaaag gtaaaacctt atttgattgg tccttagaca gcttgcttga
     1261 ttataattca catgtagaaa agtatgtttt tgttgtgcgt aaggaagatg atgctactgt
     1321 ttttatcaaa gaacattgca gtctgtatgg aattgaaaat gtcgaggtag ttgaaattga
     1381 ctatatgact gatggacaag ccactacatg tatgcttgca attccttatt gcgcacctga
     1441 taatgcaatt atggtttata acatagatac atatgttgag ccaggtgaga tgaaatacgt
     1501 tgatatttct ggagaaggac atattccgtg tttccatgcg gacggtgatc attggagttt
     1561 tgccaagttg aacgaatatg gtgatgtagt agaagtaaga gaaaaagtta gaatttctaa
     1621 caattgtact ttaggtgcat attatttttc ttctgcaaaa ttatatgcat cactctacga
     1681 agaatattat gcagatgaaa ggcatctgga aaagaatgag aaatatattg cgccgctata
     1741 taattatatg atagagaagg gcatgaaggt aaccattagt atagtagata aatcaaaagt
     1801 tcacgtactc ggtactccag aagagctcga tgtgttctta aatgaatagg gaaaatataa
     1861 tcatagaaaa tgttaattat aaagctgcgt gtgtggggga gtactattcg atagatattc
     1921 taaaaatact aatggctatt ttagttgttg ttcgtcattt aggtcaaaac tatttagctc
     1981 cagattaagc atttagtatt ggtattgttg caggactgag tacggttgcc gttcctactt
     2041 ttttcatcat cagttccttt ttgttgtgta gtggtgaagt tacatccgct aaattatcta
     2101 aacaaatcta cagagtactt aaaatttaca ttatttggtc tatattatat attccattag
     2161 tattaaagaa ttgggtacag tcagatttta gctggaaaac agatgtatta ttgtatatca
     2221 gagattttct gtttattggt agttactatc acctgtggtt cttgcctgca ctggcttttg
     2281 ctctatggtt agtacgcaaa agccaggttt ctaaaataaa gtttctcagt ggtttgttgc
     2341 tatgcgtagt aggtttagca tttgaatctt attggtttat attatctggg aatgattcca
     2401 tcgagtatat tagatattgt tataaaagcg tgtttgtaac aactcgtaat ggattattta
     2461 taggtgtacc atgcgtcatt attgggcaac acttagcctt ccacaaaaac actatactta
     2521 ataaggtctt taaataccgt aattttatct tatgtgctgt tttagctata ggatgcttat
     2581 tagttggtga aagtttcatc ataaatcaaa tagctttgcg agaagtcaat aatttgttaa
     2641 tattttccat tccattagca ctggtagttg tatcagtatc actgagccta aagatagtac
     2701 atttataaaa ataacagtac atctgcgaag aattagtacg ttattatttt gtttacatcc
     2761 gtatataata gctgctttaa attttatttt cccaaaattg gggattacaa attcattaat
     2821 agaaatgact tgtggaatga taataatttt aatttctaca gtttttgtgt atgcgtgtag
     2881 tgtgaaattt accttgctga aaaaattata ttagattagt aactggtaca tgtaaatttg
     2941 ttgaaattat agcagcatag agataggaat tctgttgagt tttattttcg ctggccgagg
     3001 ttattcatgt tagagtgggt acctcctttt acgaaacctt cttgatggta ctcatttagc
     3061 gcatgatgaa aggagagttt atgaagaaaa tcatatcttc tattttggtt aatgtgttat
     3121 ctgcattgta tcaaatattt gggtttcgat tttaattgca gttttggcta tgtttttata
     3181 cttgtttgcg atggctgagg agaaatcagg attaggtttg aaaaaatctt ttcttgtctg
     3241 gattcggtat ttcagaaaat caagctcatt tagaaaaatg tttttgcttg tgttttttac
     3301 aaatatgatt ctttttagaa cactgatgga tagatatgtt tggctaaatc ctttgtctaa
     3361 tgtgtttggt agttggtgga tttactctgt
//
LOCUS       DADXTG010000182         3364 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.185, whole genome shotgun sequence.
ACCESSION   DADXTG010000182 DADXTG010000000
VERSION     DADXTG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.185"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWI57_002572"
     CDS             179..775
                     /locus_tag="IWI57_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBC2450669.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWI57_002573"
     CDS             899..2698
                     /locus_tag="IWI57_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBC2450670.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWI57_002574"
     CDS             complement(2976..3188)
                     /locus_tag="IWI57_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450671.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          944 a          589 c          605 g         1226 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtt
//
LOCUS       DADXTG010000183         3326 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.186, whole genome shotgun sequence.
ACCESSION   DADXTG010000183 DADXTG010000000
VERSION     DADXTG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.186"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IWI57_002575"
     CDS             <1..355
                     /locus_tag="IWI57_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450672.1"
                     /translation="KDQRVAEHNNKKMSLTKNIYSMSDEELYKEFGDLSGEQLEESIP
                     GISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAISIKDLKELLNSKR
                     NELKQIKETNSVELE"
     gene            369..1058
                     /locus_tag="IWI57_002576"
     CDS             369..1058
                     /locus_tag="IWI57_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450673.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1183..>3326
                     /locus_tag="IWI57_002577"
     CDS             1183..>3326
                     /locus_tag="IWI57_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBC2450674.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIA"
BASE COUNT         1120 a          445 c          710 g         1051 t
ORIGIN      
        1 aaaagatcaa agagtagctg aacataataa taaaaaaatg agtttgacca aaaatatcta
       61 ttctatgtca gatgaagaat tgtataaaga atttggtgat ttatccgggg aacagttaga
      121 agaaagtatt ccaggaatta gtgactcaga taaaattgtt gtcttgtctg ctggtgggca
      181 attaatggga gatggcaaat ccgaattagt tagttataaa caaaatcaca gacagtttat
      241 tgatgttaat actgattcca acgctatttc gattaaggat ctaaaagagc ttttaaatag
      301 taaaagaaat gaattaaagc agataaaaga aactaattca gtggaattag agtaaaggaa
      361 gattcattat gaaaaagatt tttggtgttt ctgttgggct acttatgatg ttaacatttg
      421 gaaatgcagt atatgctgct gagaaagaag ttatttcaga tccagagcag tacgcctcac
      481 gatactttgc tgattttgaa gaagaagata aagtacttgc attgccagaa ggtggttttt
      541 taaaagggga agctaaaatt gtatctatgg ataatccaga tataattcaa ggttcatata
      601 attcagaaac tgatcctgct tcagtttcag tgacagaagc aaaaaaaatt gttgctgaaa
      661 aattcaaaaa tgatattgaa actactccaa ttatgcctcg aggagcattt ccacccgatg
      721 ttggtactag cggacacctt cttgtactag caggaggagg aagctataaa tcacagccat
      781 ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa accagctgat ggtacaggca
      841 actttttacg ttggaaatcc tacattgacg atggaagagt aggaaatgta aatcaagcca
      901 acgcaactaa atctggttct attcaaggaa tggcattgta tgctcgtgaa ggattcaaat
      961 actttgcacc aaacgggcct ggttggacaa tgaatatagg tatttattac acttataatc
     1021 ctgttgcagg tacgtattat gttgttcaaa atatttaata aaacagcaca aagagatttc
     1081 tttgtgctgt tttattattt ttagttgttg acaaacacta aaatataaaa tataatcaac
     1141 acataaacga acgtatgttt ggcaagaaag caggggattt ttatggatga gatggtttta
     1201 agtactcaga aatggctaaa caagaaatac agtaatgtta cagggtttga taaggttcca
     1261 gaaaatgggc gaacagggtg gcctacaatc tatggactta ttgaaggact acaagtagaa
     1321 cttggtatta ctaatctagt agctaatttt ggacctacca ctgaaaaaat gtacgataat
     1381 caggttactc ctaaatgggg caaaaatcta cctaaaaata ttgtatttct tattcaaggt
     1441 gccttttggt gtaaggggat taatccaggt ggcttcgatg gggtatatac tccatattta
     1501 gatactgctg taaaagagtt gcagactgat gcagggttta atggaagcgc tgtaactgga
     1561 gttttggatg cgaaatgggc caaagcgtta tttgatatga gtgcttttgt tcttgttccc
     1621 ggcggagata gtaaaattag aaaaatgcag caatggttaa atgtaaatta tttagaatac
     1681 acgggaataa tgccttgtga tggtatttat caaagaaatt caaatcaagc attaattttt
     1741 gcattacaag ctgaactagg atattctccc agtgaagcaa cagggtctta tggtaatggg
     1801 acaactagta aaacttatcc tgtgtctgaa ggtaactcgg gaaactatgt aagaataatt
     1861 caatatggat tatatgttaa tggttatttt gaagacggta cattcgatgg tattttcact
     1921 aaatatatgg gtttagaagt actggcattc cgaaaattta tggtattgcc tgaatatact
     1981 gaaatagcag atgttgttgt aataaaagga ctattgtcta gcgctggaga tattagaaga
     2041 tctgcagatg gcgcagatac ttctacgcaa ttaactagat ctcaaatcca aacattggtt
     2101 gataatgata taaaaatagt tggtagatat cttactggta ctgttggaat tggaaaagat
     2161 gaacgaaata agtatctaac tacagaggaa ttgaataata ttttttctaa aaatttatct
     2221 gtgtttccta tctatcaaga tggaggtgca gcattagctt attttactta tgatcgtggc
     2281 ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc taaatatacc attaacaaca
     2341 gttatttatt ttgctgttga tctagacatg ttaggggaag aaattcttgc atatgctgga
     2401 gaatatttta agggggtatc tgctgtaatg tcatatagcg ggtatcaaac gggtgtttac
     2461 ggaacaagaa atgtttgttc tcaaataatc aataacgggt atgcttcgtt tgcatttgtt
     2521 tcagatatgt cgactggtta tagtgggaat ttaggttttc ctatgcctag acaatggtct
     2581 tttgatcaat ttgttgaatt taccatagga agtggcaacg gagctgttgg tatcgattta
     2641 atcgctacat cgggtagaga tagcggattt aatgagttat caaatgatac taataatgat
     2701 aattacattg ccaaatacaa tcagaaggtt ataaatcaga tggtacgtgc gtatcaatat
     2761 ctatcacaag ctggtcttga taatccttgg aaccctcatt tgacatttta tagatatgta
     2821 aattattctg gtttgtcatg ggacatcatt tcttctcctg taacagagca tgatagaaaa
     2881 atttatgatg aatataggaa taaattaact aatagcgagg gcctgtataa ttattttata
     2941 gatcctaata ctggttcaat aataggtttg cctcacttaa ttgtgacgtt acaatcacag
     3001 atgtttctag ccgacacaat caatgatagt gtttctgact ttgcaggttg gttaggagat
     3061 ttaatgactt gctggggtga agttaagaaa ttaggaatac aaatggagca aggtgtattt
     3121 gcattagtag gtacggctgc aggtggaaca tttagtctag aagatttata ccaagatgtt
     3181 gacgcggtaa atgtttatac cttagcaaaa agaaattctg attcaacagc tttatcggct
     3241 ttaaaaacgt actattgtga taagggtgct attgatagat ttaatcaatt tgttaagaac
     3301 agatttggtt cttcagataa aatcgc
//
LOCUS       DADXTG010000184         3298 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.188, whole genome shotgun sequence.
ACCESSION   DADXTG010000184 DADXTG010000000
VERSION     DADXTG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.188"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IWI57_002578"
     CDS             <1..387
                     /locus_tag="IWI57_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBC2450675.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
     gene            491..715
                     /locus_tag="IWI57_002579"
     CDS             491..715
                     /locus_tag="IWI57_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450676.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            832..1329
                     /locus_tag="IWI57_002580"
     CDS             832..1329
                     /locus_tag="IWI57_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBC2450677.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            1304..1810
                     /locus_tag="IWI57_002581"
     CDS             1304..1810
                     /locus_tag="IWI57_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2450678.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1794..>3298
                     /locus_tag="IWI57_002582"
     CDS             1794..>3298
                     /locus_tag="IWI57_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2450679.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT         1040 a          598 c          691 g          969 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatttata atatcaggaa cttacctatc catatataac tggtttctat caaacgctca
      541 atcactggtg cttttagcaa tcgttgtgat tggcttgtat cttggcttca agcgtgagtt
      601 tagcaaactg attggctttt taattattgc gattattgcg gttggcttag tcttcaacgc
      661 tgctggagta aaagacattt tactagagct attcaatcgc attattggtg cttaaataaa
      721 accgttcttt tgtggaatat aagtggtttt cttatgttcc gcaaaggaat ggtacaccaa
      781 acgaagtgcg gtagggattt ttgaatctct acaaagaaag gacgtgaata tatggacgat
      841 atgcaagtct atattgcgaa tttaggcaaa tacaatgagg gcgaattggt cggtgcgtgg
      901 tttacctttc ccattgactt tgaggaagtc aaagagaaaa tcggcttgaa tgatgaatat
      961 gaggaatacg ccattcatga ctacgagtta ccctttacgg ttgacgaata cacttccatt
     1021 ggcgaactca atcgactatg ggaaatggta tcggaattac ccgaagaatt acaatcggag
     1081 ctatctgctc tgctcactca tttttcaagc attgaagaac taagcgaaca tcaagaggat
     1141 attatcattc attccgattg tgatgatatg tatgacgtgg cacgctacta cattgaagaa
     1201 acgggtgctt taggcgaagt accagctagt cttcaaaact atattgatta tcaagcctat
     1261 ggtcgggatt tagacctttc aggaacgttt atctcaacca atcatgggat ttttgaaatc
     1321 gtctattaaa tctgtcggta cattactact ggcagatttt ctattttacg gggtggctca
     1381 atcagctacc cctatttttt atgaaaggat tgattacatg aagaaaatac gaagctatac
     1441 cagtatctgg tctgtggaaa aggtactgta ttctatcaat gattttagac ttccgtttcc
     1501 cataaccttt acgcaaatga catggtttgt cgtgtcactc tttgcagtga tgatacttgg
     1561 caacttgccc cctctttcca tgatagaggg agcatttctc aaatactttg ggattcctgt
     1621 ggctttcaca tggtttatgt ctacaaaaac ttttgatggt aaaaagcctt atggattttt
     1681 gaagtctgtc attgcttatg cactgcgacc aaagctgacc tatgcaggaa aaaaagtaac
     1741 gcttggcaga aaccagccac aagaagccat tacagcagtt aggagtgaat tttatggcat
     1801 atccaattaa atacattgaa aacaatctcg tctggaataa agacggggaa tgttatgctt
     1861 actatgagct tgttccttac aattactcat ttctaagtcc agaacagaaa atacaagtgc
     1921 atgattcttt cagacagctt atcgcacaaa atcgtgatgg caaaattcat gctttacaaa
     1981 tcagtacaga atccagcata cgttctgcac aagagcgttc caaaaatgaa gtcactggca
     2041 agctcaaagc ggttgcctat gacaaaatcg accaacagac agacgcttta atatccatga
     2101 ttggcgaaaa tcaagtgaac taccgtttct ttatcggctt taagttgctt ctcaacgatc
     2161 aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa tgctttgtct gattttgtct
     2221 atgatgtgaa ccataagctg atgggcgatt ttgttagtat gagtaatgat gaaatcctgc
     2281 gttttcagaa gatggaaaag ctcttagaaa ataaaatctc tcgtcgtttc aaaatccgca
     2341 ggttagataa ggacgacttc ggctatctga ttgaacacct ttacggacag acaggcactg
     2401 cctatgaaga gtatgagtac catctatcaa agaaaaagct ggataatgaa acgctgatta
     2461 aatactatga cttgattaag cctactcgct gtttggtgga agaaaaacag cgatatttga
     2521 aaatccagca ggaagatgaa accgtctatg tagcttactt taccattaac agcattgtcg
     2581 gagaactgga cttcccgtcc tctgaaatct tctactacca gcaacagcaa tttacattcc
     2641 cgattgatac gtcaatgaat gtggaaattg tagcgaatcg taaagcccta tctactgtcc
     2701 gcaataaaaa gaaagaactg aaagacttgg ataaccacgc ttggcaaagt gataatgaaa
     2761 ccagctccaa tgtggcggaa gctctggaaa gtgtgaatga gctggaaacc aatttagacc
     2821 aaagcaagga atctatgtac aagctgtctt atgtggtaag ggtatcagca aatgatcttg
     2881 acgaactcaa acgtcgttgt aatgaagtga aagattttta tgacgattta agcgtaaaac
     2941 tggtacgacc atttggggat atgctcggct tacatgaaga atttttacct gccagcaagc
     3001 gttatatgaa tgattatatt caatacgtga cctctgattt cctcgctggt ttaggttttg
     3061 gtgctactca aatgctgggg gaaaatgagg ggatttatgt tggctacagc ttagatactg
     3121 gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg ggttaagggt tcagtaacca
     3181 atgcgttagc gtcggctttt gttggttcgc tgggtggtgg taaatccttt gcgaataacc
     3241 ttatcgtcta ttatgcggtg ctttatgggg cacaagcagt gattgtagac ccaaaagc
//
LOCUS       DADXTG010000185         3278 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.189, whole genome shotgun sequence.
ACCESSION   DADXTG010000185 DADXTG010000000
VERSION     DADXTG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.189"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..83)
                     /locus_tag="IWI57_002583"
     CDS             complement(<1..83)
                     /locus_tag="IWI57_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBC2450680.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            complement(132..215)
                     /locus_tag="IWI57_002584"
                     /pseudo
     CDS             complement(132..215)
                     /locus_tag="IWI57_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            complement(625..1419)
                     /locus_tag="IWI57_002585"
                     /note="aph(3')-IIIa"
     CDS             complement(625..1419)
                     /locus_tag="IWI57_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBC2450681.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(1512..1763)
                     /locus_tag="IWI57_002586"
                     /pseudo
     CDS             complement(1512..1763)
                     /locus_tag="IWI57_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2008..>3278
                     /locus_tag="IWI57_002587"
     CDS             2008..>3278
                     /locus_tag="IWI57_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBC2450682.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFL"
BASE COUNT         1036 a          695 c          559 g          988 t
ORIGIN      
        1 tatttatctg cgtaatcact gtttttagtc tgtttcaaaa cagtagatgt tttatctaca
       61 taacgcattt ggaataccaa catgacgaat ccctccttct taattacaaa tttttagcat
      121 ctaatttaac ttcaattcct attatacaaa attttaagat aatgcactat caacacactc
      181 ttaagtttgc ttctaagtct tatttccata actttagggt taaccatacg caagaccaat
      241 cactctcgga caatactcat gattttaatg ataaaatcca tgttaaaccc atagataaga
      301 aatacacctg caataaccgt tacctgtttg tgccaattta aaaatgcacc acttttttat
      361 aattaaaacg gttgaaaact gtaccactaa taactcacaa tagagagatg tcaccgtcaa
      421 gttaaatgta caaaataaca gcgaaatttt taaatctatt tcttatcgat acaaattcct
      481 cgtaggcgct cgggacccct atctagcgaa cttttagaaa agatataaaa catcagagta
      541 tggacagttg cggatgtact tcagaaaaga ttagatgtct aaaaagcttg tagttaaagc
      601 tttttagaca tctaaatcta ggtactaaaa caattcatcc agtaaaatat aatattttat
      661 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
      721 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
      781 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
      841 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
      901 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
      961 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
     1021 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
     1081 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
     1141 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
     1201 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
     1261 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
     1321 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
     1381 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
     1441 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
     1501 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
     1561 ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg atagaattta
     1621 caagctataa ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt
     1681 gcccattcta tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta
     1741 catacggcga tatcttctat ataaaagata acccgtggtg ctagttaatt ttttcctaga
     1801 ggaaaatgtc taaacatgtt ggtaaatcaa caaaaaaacg catatagtag tctcaaaact
     1861 cccaagaaat gggctctata atttatggga ctgatgaatc agaattgatt cttcagcccc
     1921 attttcattt taggtattaa aaaacatatc gttctctagt atgattaaat caccacaaaa
     1981 taaacaactg agaggacgat acgctatatg aatgattcta tcaaaaaaat gctgagaata
     2041 acagagaaag atttgatgat tacagaggtc tcttacgaga cccttcagaa gaaaaagacg
     2101 ttggtcgtcg atgctgttct ctcgcctact cctcgtgctt gtagaagttg tggttctact
     2161 gtggtagatg gaaacgggaa agcaattata gtgaaaaatg gaaaaaagga aacgattgtc
     2221 cgttttgaac aatacaatca tatgcctttg gttatgcgcc taaaaaagca acgctatacc
     2281 tgtaaaaact gtcgcaccca ttggacggct caaagttatt ttgtccaacc cagacattca
     2341 atcgcaaatc atgttagata taaaattgct tctttactga ctgaaaaagt atctttatct
     2401 tttattgcga aaagctgtca ggtatctttg accaccgtta ttcgtacatt gaaagagttt
     2461 aaaagctatt taccaaagca atctaagaag atcctcccca gagtattgat ggttgacgaa
     2521 tttcgttcgc atgcttccat agaagataag atgagcttta tttgcgcgga tggcgaaaca
     2581 ggaaaattaa tagatgtttt gcctacgcgt aaattacctc gattaacaag ctatttctta
     2641 ggttgtacca atccagaaga agtagaattc ttggtgacag acatgaacgc cgcctacttc
     2701 cagctcacca aacgtgttct gccaaatgcg aaagtcgtga ttgatcggtt tcatattgtc
     2761 aaacacatga atcaagcgtt caatgagttg cgtatccgtg aaatgaatga acttcgtaaa
     2821 gccggacaga aaagccaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat
     2881 cgtgcaaaca tcaaccatta tgaatacaaa acatggaaaa gtttccgagc accaaaatac
     2941 ccatttctta ctgaagcaat gatgattgat cgattgcttg agttttctcc gcccctaaag
     3001 gaggcgtatc ccttttttca tgaattagtt gaagcctttc gagacaaaga ccctgactta
     3061 tttttctcct tattggcaga acttcccgaa acgttagatg acagctttcg ggaaaagctt
     3121 caaaaccttc tgacctatga agaaggcatc accaacgcaa tgatctatcc ttattccaat
     3181 ggaaaaatag aagcgaagaa tacccacata aagacaatga aacgagtatc ctacggattt
     3241 aaatcatttg agaacatgag aattagaatc tttttgat
//
LOCUS       DADXTG010000186         3273 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.190, whole genome shotgun sequence.
ACCESSION   DADXTG010000186 DADXTG010000000
VERSION     DADXTG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.190"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..761
                     /locus_tag="IWI57_002588"
     CDS             <1..761
                     /locus_tag="IWI57_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBC2450683.1"
                     /translation="PFDYAKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQR
                     TYSSNANVFVQLLNVDIPNYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAI
                     VKSVASGEGVIHALNTMRLDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSY
                     LETGIEEYNVLTAKDERVCRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLP
                     KLGKDIDEEYDRLFGDLLDEFAHDSFGINLKRRK"
     gene            763..1080
                     /locus_tag="IWI57_002589"
     CDS             763..1080
                     /locus_tag="IWI57_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450684.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            1207..1830
                     /locus_tag="IWI57_002590"
     CDS             1207..1830
                     /locus_tag="IWI57_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBC2450685.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            1845..2735
                     /locus_tag="IWI57_002591"
     CDS             1845..2735
                     /locus_tag="IWI57_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBC2450686.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            2758..2973
                     /locus_tag="IWI57_002592"
     CDS             2758..2973
                     /locus_tag="IWI57_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450687.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            2985..>3273
                     /locus_tag="IWI57_002593"
     CDS             2985..>3273
                     /locus_tag="IWI57_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450688.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLG"
BASE COUNT         1228 a          446 c          654 g          945 t
ORIGIN      
        1 caccatttga ctatgcaaaa gtcaatcgct tattacaaat acgatcagat gtatttaaaa
       61 ttcttgcaga atatgcgatg gatgcagatg tgaatcaata tttttctgat cgcttagagg
      121 aaattcttca aagaacatat tcttctaatg ctaatgtttt tgttcaactt ttaaacgttg
      181 atataccaaa ttatttacca gaaaatgaaa ttaaacatta tttgaactat ccatggtcag
      241 gaaagacatt ttcaagaaga ctttggggaa atatttcatc gcttgaacag aaactctcta
      301 atgctattgt aaaaagtgtt gctagtggag aaggcgttat acacgcatta aacaccatga
      361 gattagactc agaaatttgt gacatgttta agttagaaga atcaaagtat aacaaagcga
      421 tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca agatggtatt gaaaaatcat
      481 atttagaaac aggtattgag gaatacaacg tattgactgc aaaagatgag agagtttgcc
      541 ggatttgtgg aggaaaggca agtaagaatc cctataaact gaaagatgct gtcataggtg
      601 aaaatcgagc acctttccat agtcgttgta gatgtacgga tgttcctaat ttaccaaaat
      661 taggaaagga tattgatgaa gaatatgacc gtttatttgg cgatttatta gatgagtttg
      721 cacatgattc ttttggaatt aatttgaaac ggaggaagta gaatgaaaga tttttttgaa
      781 gcagtactaa caattaatgt aaatgctgat attgcagaag cctacaaaac agctattgag
      841 tctgagaacc atcctaatgg cttgagagac cattggaatg gcaattatgc ctacgtggtt
      901 attggcgatc aaactgttaa ttatcaagat aatactccag ttgataagaa taccgttaat
      961 ttaacgattc aattattatc tcattcatta ccaaatttaa aagaaacagt tgattggtat
     1021 gaaaagatgg gatgtattgt tgttagaact gactacaaag aaggaaagtc tagtaattag
     1081 gcttttttat tttgtccgaa atgacgttaa actagcgcaa tactgggctt ggttgaatgg
     1141 tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc gtggggcgaa
     1201 aggagaatga gcatgaaaca caaatcatta atgccaatga atttgcaata ctttgccgaa
     1261 ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc caatgaagaa
     1321 gctcaaaaat ttattcagtc acaatctcaa agtgttgccg acaaacaatt ggaagcttgg
     1381 aaacaaaata acttagataa aattaaacag gataccatca aggaatatga ggaatctaag
     1441 aaaaacaagt cacctgagca aattcaactg gaaaaactac aagctgaatt tgaagcagaa
     1501 aaagcgttgc gtgtgacaag tgataataaa gcatttgttg cagaacaaat tgctggatta
     1561 gaactagatg gagagttaaa agagtctatt tctcaattta tgctaaataa tcttgttagt
     1621 tcggatacag atttcactaa gaatgctgtt gaaggtttta caagtgtctt gaatgcaatt
     1681 aatgagaaac atgcagatgc attaaaagaa ctacaaatga agtctgcatt tggtggaact
     1741 caacaatcga ataaccaggt tcagcagaac aatgaaacat taacaaatcc agaagaacaa
     1801 ttaggacaaa ttcttcaaca atttaactag gagagtgaaa aattatgaaa aaaacatctt
     1861 taaataattt agagtatttg gatatttcac cagcgattaa tgctatgcaa gtaccaaata
     1921 cacctttttt aagctattta tttggtgctg gaaaaacaga gccagcaaac tcgacagaaa
     1981 ttaaatggcg tgaatatgat atcaacaacg atgattcttc tgaaaaactt gagggcggag
     2041 aatatccaga tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa atttttagaa
     2101 aatcaacctc tgtatctggt acattagatg ctattaatgt gaatggagtc ggaaatgaat
     2161 taactaatca agtagcacta cgtggtatgg aaatgaaaat tgatttgaac cgaaaattga
     2221 ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat gggattttga
     2281 acttgatcaa ttcagcaaat aaggcagaaa cagccactgc gggtgcagta acaagaaaag
     2341 atatcgatac tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa aaactatgct
     2401 tgatttctcc agatatgcag gagttaatga ctgatgagtt agatggaaaa tcaacaaaaa
     2461 ttgttcagtt cggggaaaga gtaacttttg gattgcaaat tggaaatatc gtgtctaatt
     2521 acggtacagg tattgctcta ctagaaccat cattgccaaa aggaacaatt gccgcaatcg
     2581 atactaatta tgtgaaacta cgtccattac gtgaatggag agcagaagaa cttgcaaaaa
     2641 caacggattc tagacgtatc ggtcttgtag gtgaatactc tcttgaatac aatgcttcaa
     2701 actctggggc aattttaaat ttaaagtctg aataaaaggg agttagtact cccttttttg
     2761 ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt cgtgaaatat
     2821 agagtaggta aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg tagatttatc
     2881 acagcagatt caaataacga atttatcatt tctatagatg ataaagaagc aattgcaatt
     2941 ttggaagatg caattgatgt taatgaaatt taggaagtga tctgatggat gaatcgctaa
     3001 aaacggaaat cattgagtct acaaaagaag attttccaga tttgagtgaa gaacgcataa
     3061 ctaatttatt agaaataatt ttgctagaaa ttgaatcata caacacttgt aaaaatgata
     3121 tttcatggga aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata ataaaaaatg
     3181 aatcagaaaa aacagtatct tcagttagac gtggcgatac aacgattagt tatgcttcaa
     3241 caacaaatga tgttagtgag ttacttctag gct
//
LOCUS       DADXTG010000187         3272 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.191, whole genome shotgun sequence.
ACCESSION   DADXTG010000187 DADXTG010000000
VERSION     DADXTG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.191"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            162..734
                     /locus_tag="IWI57_002594"
     CDS             162..734
                     /locus_tag="IWI57_002594"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBC2450689.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1207..1779)
                     /locus_tag="IWI57_002595"
     CDS             complement(1207..1779)
                     /locus_tag="IWI57_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2450690.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1795..2400)
                     /locus_tag="IWI57_002596"
     CDS             complement(1795..2400)
                     /locus_tag="IWI57_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBC2450691.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2598..>3272)
                     /locus_tag="IWI57_002597"
     CDS             complement(2598..>3272)
                     /locus_tag="IWI57_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBC2450692.1"
                     /translation="HFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTT
                     IYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYL
                     NNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIPA"
BASE COUNT         1006 a          651 c          530 g         1085 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccat catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct
      241 gtgagaaatg ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact
      301 tatcttcaat catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa
      361 tgactgtgga ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag
      421 aaggaatgat tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat
      481 ggcttaaaac caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg
      541 ctggaatcga atctttccat gcctcattga aaaaagaaga agtctacacg actagctact
      601 cagattttga agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa
      661 atcgaatcca tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag
      721 aaaaaatggc ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa
      781 tatcacaata ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat
      841 ttggttctta aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc
      901 aatagagtca atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg
      961 acttacgctg aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat
     1021 gatggtatcc taatatagta agcgtggcta gcataccttt aattacttta ccttttacag
     1081 ttaccataca cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac
     1141 atgctaatag actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact
     1201 tcttttttaa ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc
     1261 ggttatagct tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct
     1321 cttcttcggt gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct
     1381 tgcaaacatt tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac
     1441 actcaacaac gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt
     1501 caaaaccttg atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct
     1561 cgtgttacga gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc
     1621 agccaacagc tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa
     1681 aatattcttc ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg
     1741 agaagcggtt gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa
     1801 atcatctatc gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat
     1861 atataactcg cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc
     1921 caaatcgttt atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt
     1981 ccaatctaca actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact
     2041 tcggccattt ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat
     2101 ctcttcaaaa gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat
     2161 aactggtgca aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa
     2221 atcgtaaata tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac
     2281 ttcgatttca tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt
     2341 ttcttcaacc ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat
     2401 gcgaaatctc cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg
     2461 cgattttgtt tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg
     2521 tctctatttt gcacagtcta gttggttctg ttgcaaagtt ttaaataaag aataaaatcc
     2581 cttacggtat ctatgattta agctgggatt cccaataata ccttgatttc agtacagacc
     2641 gaaaacccga agagagtgcc ttcttttcgg gttttcttat ataatcctcg aatggcttcc
     2701 atgcctttaa tcgtggtaga ggcagtgcgt aaacttcgat agaatttatt gcgtctcttt
     2761 actggacgat ggtcttgttc aatcaaatta ttcaggtatt taatggtacg atgttctgtc
     2821 ccttgataaa agccgtattc ttttagtttc ttaaaggcac ttgtaataga gggggcttta
     2881 tctgtgacta caaccttcgg ttcatcaaac tgcttcacta accgcttaag aaaagcatag
     2941 gctgcttgtg tgtcccgttt tttacgtaac caaatatcca aggttaaacc atctgcatcg
     3001 atggctcgat acaaataatg ccattttcct ttaattttga tgtacgtttc atccattttc
     3061 catgaataaa aggatttttt atttttcttt ttccaaattt gatagagtag tttgccatat
     3121 tcttgcaccc aacgataaat cgtcgtatga gaaacgttaa tgccacgatc atataagatt
     3181 tcttgaactt cacgatagct aaggttataa cgaagatagt agcccacggc tacaataatc
     3241 acatcctgct gaaattgctt tcctttaaaa tg
//
LOCUS       DADXTG010000188         3219 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.192, whole genome shotgun sequence.
ACCESSION   DADXTG010000188 DADXTG010000000
VERSION     DADXTG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.192"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..169
                     /locus_tag="IWI57_002598"
     CDS             <1..169
                     /locus_tag="IWI57_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450693.1"
                     /translation="NLINELVSRCGTFFNTITIDEQILFPKAYLPDEADGVQHIRFTL
                     EFPQYIEWSER"
     gene            170..778
                     /locus_tag="IWI57_002599"
     CDS             170..778
                     /locus_tag="IWI57_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBC2450694.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            778..1119
                     /locus_tag="IWI57_002600"
     CDS             778..1119
                     /locus_tag="IWI57_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450695.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            1361..>3219
                     /locus_tag="IWI57_002601"
     CDS             1361..>3219
                     /locus_tag="IWI57_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBC2450696.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFA"
BASE COUNT         1113 a          418 c          689 g          999 t
ORIGIN      
        1 aaatttaatt aatgaattag tctcacgttg tgggactttt tttaatacga ttacaatcga
       61 tgagcaaata ttatttccaa aagcctattt acctgatgaa gcagatggtg ttcaacatat
      121 tcgttttaca ttagaatttc cacaatatat tgaatggagt gaaagataaa tgggagaaaa
      181 aagaagtaaa accggaatta tttctgtcga aaaacctact tggtttcctt tggaagatga
      241 gacagggaat tttccggtat atggtactgc gttgccaatg ggaacagcag taagtatcaa
      301 accaacagct aattatgaaa caacacaaga ttatggggat tcagtggttc aagatcaatt
      361 tacagctttt ggtggtgcag aagttgaact ggaagcaaat ggatatactc ataaagtttt
      421 atctacaatt actggaggga aaattgttaa aggtggtgcc ttgcgttctg gagaagatat
      481 tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg aatgggaaat accgttatac
      541 ggtcttttat aaaggacagt ttgctctaga ttcagatgaa acatcaacga ttgaaggaag
      601 taaagtaagt tttactcatc caacttggaa gggttcattt gtagatgtac caggacttgg
      661 atatatgtat tcagttgatg aagatgatga aggcgtggat aaagcaatga tcggaaattg
      721 gtttacaaaa gtagcaattc caattgaaga agcagaactt tcaggaggta cagaataatg
      781 tcaaaatatc aaacaacgat taaattaacg aaaaaagatg aagaaggtaa atatgagcaa
      841 gtacaattta aatctgctga atttttacca ggaacagttg tagaagatgc tgcaggagtt
      901 atggaagaaa tgcaaacagc gactgataaa caatcagtta aaaaagcttt aagtcgtgct
      961 tattcattta ttgcagatac tttatttgag ggacaattta ctggtgaaga ttattgcaaa
     1021 ggaattgatg ctcgtgagat tgcctcattg acaggaaaat tattgaagtc tgttactgca
     1081 ggttttgatg aaacttatac agagacgaaa aaaaagtaag tgaggcgctc aagtcacctt
     1141 catttaagta ttcgattact taccgagaat tagatataaa gacgcaatta ctcgaagcag
     1201 gttggacgtt accagaaatt gaacgtactg acttggatga gttaatgcgt ctttttgctt
     1261 ttagagatgc ggtaaaagag catgaagatg ttgagtacta tgataatttc actcaatttt
     1321 aggaggtgat actttgaata acgatgacct aattctgaag atgatactag atgaatctgg
     1381 ttttactgcc ggtatgaata atgctgtcaa aaagttgaac tcttttgatg aaacaatcga
     1441 aagaaaaagt agaaatagtg gcagttcatt aggtagtatt tggaaaatat ttgctggaag
     1501 ttttctagct agcggagtaa ctagaatcgt aggagctggc tttgatctaa tcaagggttc
     1561 cataagtgga gcggttgatc gagtagatac gatgaataac gctctacgaa atttccaaaa
     1621 tatgggattt agtaattcag aaattatgaa gaatatcgga aagaatgggc ttttatctca
     1681 aggtattcaa ggacttccta ctgctttgaa tgatgcgata agtcatgttc aacttcttgc
     1741 ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt aaagctttaa atgatggaat
     1801 tcttggtttc ggtggttcaa ctgatcaagt taatgaagct gttattcaat tatctcaaag
     1861 tttctcaaac ggaaaagtag acgcacaaac ttggaactca atgatcaacg ctcaacttgg
     1921 acctactctt tctgctattg ctaaaaagat gggaattaca atgggagatc tgaaagaagg
     1981 tttgtctcaa ggtaagattt ctgttgaaga attccaaaat caattaatag aaatggatac
     2041 caaaggtggc ggaggactta aatcattaag tcaaatcgct aaagattcta ctaagggaat
     2101 taaaacctct atacaaaatg caaaaacagc tgttacacgt ggtgttggtg aggtaataga
     2161 aggattaaat aaagcattag tagattcaga tttaggtgga tttaagggaa ttattgataa
     2221 agtgggcagt tcaatggaat cgttcttaaa agtaattgct gcaaatattc ctacagcagt
     2281 atcattttta agtaatttat ttaacgaaat tcaaaaattt ggttctgctt tgaaattcat
     2341 gatgcccttt cttgttcctg cagctaccgc ttttggagcg ttcatgtttc aacttaaagg
     2401 tataccagca ataataaaaa gcttcaataa ttttaagaat gccataattg gcgttggaaa
     2461 ttcattaaag attatggggg caatagctgc tgctaattca tttgttttaa ttattggtgc
     2521 agtcgttggt gcaatagctg ttttcggtta ctttatggca accaatgaag agtttagaaa
     2581 caaggttata tctatttgga atgatgtgaa agattccgta attggtgtat taaaaaatat
     2641 aaaggactgg ggaattgata cttggaattc tgctaaagaa atggcatcaa atgcagttga
     2701 gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt tctaacacct ggaaaggtat
     2761 taaggatggg gccacaggtt tatttgataa aacagtagag acttcaagaa atgcagtcga
     2821 tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt tctgatactt ggcaaagtat
     2881 aaaagattca gccagagaaa aatggaatga aattaaaggt tctattatgt cggttgctgg
     2941 tccattgatt acaggtataa agaatgcatt tttacatgtt actttttatc tagagacctt
     3001 atggaataat ttagttgaga ttggtaaaaa cgtctttgag attttaaaaa atgttatact
     3061 tgcgccggtt ttgtttatta cctccatgat ttctggtgga tgggaagaaa caaaaaataa
     3121 tatgattgct gtttggaata atattaaaga aagtgccata aatatttggg aatctataaa
     3181 gaatatattt gtaagttatt ttacaaatat ttattttgc
//
LOCUS       DADXTG010000189         3183 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.193, whole genome shotgun sequence.
ACCESSION   DADXTG010000189 DADXTG010000000
VERSION     DADXTG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.193"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2303)
                     /locus_tag="IWI57_002602"
     CDS             complement(<1..2303)
                     /locus_tag="IWI57_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450697.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3183)
                     /locus_tag="IWI57_002603"
                     /pseudo
     CDS             complement(2975..3183)
                     /locus_tag="IWI57_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          871 a          722 c          526 g         1064 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tag
//
LOCUS       DADXTG010000190         3137 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.194, whole genome shotgun sequence.
ACCESSION   DADXTG010000190 DADXTG010000000
VERSION     DADXTG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.194"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..340)
                     /locus_tag="IWI57_002604"
     CDS             complement(<1..340)
                     /locus_tag="IWI57_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2450698.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            complement(1671..2336)
                     /locus_tag="IWI57_002605"
     CDS             complement(1671..2336)
                     /locus_tag="IWI57_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450699.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2462..>3137
                     /locus_tag="IWI57_002606"
     CDS             2462..>3137
                     /locus_tag="IWI57_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBC2450700.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISL"
BASE COUNT         1010 a          506 c          566 g         1055 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaagaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gaggaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagatat catccataaa atggactttg
      421 tctacagtct gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta
      481 ttttacattc taatatattc atatgagcta attttatgat taaatggtaa gtgtttcctt
      541 gtttgtttta gtttaaacta gtgtgtaatt taaaaatcta tatggaaggg tctgtggggt
      601 aatacttaaa taaaggctag gaacctgata acatcaaggt tcctagcctt tttataataa
      661 aatgaagcga aaataatttc ttttaaagag ccgagattat cagctatata agagtgaatt
      721 tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta gctctgctta tatgtttcga
      781 atttaagaca aagagttggc aaaagatgtt gataaggaag ataagataac tgtgttatat
      841 aaactaggta actaactgta acaagctaat tttaagagat tcttattaga agacgtttag
      901 gaatataact aagaaaatga ttatacaggt aattgtcatt tcaataatgt tttctaattt
      961 tcgcgtcttt gttttaagaa aaaggccaga atcagttgga aaaaagttcg tacgatagtg
     1021 aagaggggca aaatttccta ctaatattcc cactaacaag agataaaacg gccaggttgg
     1081 tgtgattcct ctataaaaaa atgcccatgc taccgaaatc ccatagatta acaactgaac
     1141 aatttttaca atgaaaaaat tgttctgcca ttactcaaag tctcttcctc cttgtatcgt
     1201 ttatttgttt cattttatca ttatgatagg cgcttgaaca atccattttg tattttattg
     1261 agtactcttg gataaactac cagcgattat catatttatt tatgttactg taagccatca
     1321 tataaaagta aatgagcaga tcatagaaat ataaaaaaga actaaaattg atctaaatta
     1381 tgtgcagctt gactacgtac caatctatat tacttactag attggtcgtt aatctattcg
     1441 gatcgaatga gcaaaatatt tttgtgaatg tccatccttg attatgattc caaaaagaat
     1501 cattgggagg ctgtggcgta aatattatac tataagccag aaatacctat cattctgaac
     1561 ttttttattt aggaactttc atctcaaggc tttttcaatt aatttgtacc cctgtttttt
     1621 ggataagatc atgtgcattg attttttcta tctctacgtt catctcctac ttaatgccta
     1681 tctggctcct gagggcttct ttttaaggcg tcgtcatagt cttggctgag gatttcttgg
     1741 taatctgagg gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg ttttcctttg
     1801 tgtttgatta cagcatggtt tttctctttt tctacctctc cagcgtgaaa agacagccat
     1861 gtaccatggc caaagatatc attacatacc caacgttcga tggaattgtc cacgtatcct
     1921 acagggagtg tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg atcgatcgtt
     1981 tcatttttgt attgataatt gataagaaaa agatagacca aacaaaagac gagcagaaag
     2041 aaagatagtg tctttttgag tgagaagggt tgttttgatg aataagattt cttgttaaca
     2101 agaggttcat ctggcaatgt tggttctatt gattcggtag ctgattcaat agaaggaaaa
     2161 tcaatcggca gacctaaagt atgatgtaaa agtgacagca tttcaaggtc tggcattgta
     2221 cgagaatttt cccaactaga aatcgtttta tcggaaacac caattttttc gcctaattct
     2281 ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt tttctccggt gttcatcttc
     2341 ttctcctttc gattctacag tatatagatt tattctacat gacctataca cttgatagat
     2401 acgttttttt gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt aagggagagt
     2461 ggtgataatc aaaaaaaagt tcacagtttt actcacaatc atagtgaccg gtatcatgag
     2521 tggttttgcc ttggttagta taaataaaca ggtactctat cggacaaatg aatttttcca
     2581 tcaaacagat acttatactc cgattcgatt gccacaacat tttattgggc aagaagaata
     2641 tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa actgagcttt cttatatgaa
     2701 aaaaacagta aatgaatcca atgaagtaga tacatcttgg aacttcaaga tacagccaaa
     2761 gcttgaaatg acattgtatt atcaagggga tgtcaaatct tctcacgaga aggtacccta
     2821 ttctaatacc tcctttcgcg taacgtccat tgaagaatcg atcaaaataa aagattttga
     2881 aggtgaattt ttcttgatga cgagagaaaa agaagcctat gaacggttca tttcactatt
     2941 cactgatcga ttcaatcaag catttcacac tgattatgcc actaatattt ttgaagactt
     3001 tgaaaatagc tattcgtatt cggttatttc aaatacagac gtatataatt atcgagtgta
     3061 tttaactatt ggattgattc ttttattcct tttgttatca tcctttttct tattgatgaa
     3121 taaagaaatc agtttat
//
LOCUS       DADXTG010000191         3127 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.195, whole genome shotgun sequence.
ACCESSION   DADXTG010000191 DADXTG010000000
VERSION     DADXTG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.195"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(180..548)
                     /locus_tag="IWI57_002607"
     CDS             complement(180..548)
                     /locus_tag="IWI57_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBC2450701.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            698..1180
                     /locus_tag="IWI57_002608"
     CDS             698..1180
                     /locus_tag="IWI57_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBC2450702.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1217..2227
                     /locus_tag="IWI57_002609"
     CDS             1217..2227
                     /locus_tag="IWI57_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBC2450703.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2342..3055)
                     /locus_tag="IWI57_002610"
     CDS             complement(2342..3055)
                     /locus_tag="IWI57_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450704.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1055 a          509 c          611 g          952 t
ORIGIN      
        1 aattcaaagc cattcaatca gtgatttttt tcagcacaag aaagactcct tataaaaata
       61 gcagatactt tgtcaattaa gtgtttaacc agggatgata aaaaggatag gattgaacaa
      121 aacctgtata agctgatgaa tagtttcctc gccagtaaaa ggtttattcg tataaagctt
      181 tatcgccaaa atttatcatg acctttttta tcttcaatat taaatgaaat caattgttca
      241 atcaattccc agttgattac acttttccaa ggaactttga tgacgttatc cgtatatacg
      301 tagcctgctt cttggatatt tttttcaagg gcacgaatcg ctgctttttc cggtgcgata
      361 gaaaaatgat ttttagcaaa actaaatgca ataataaagg taccatggtc agtaaacata
      421 ggttggttcc attttacagt tgtgtctagc tggggatacg tttctgttat cttagcaaaa
      481 aggtctttga tcttttgttg atgttctttt tctttgatct gattgatgat cggctgaaat
      541 ttttccattc taaaacgcct ccattaagtg gtttgattta ttttaccaga aaaacaatag
      601 ttatgtataa aaaacgaaca aaacacaagt aatgttgtaa ccgctatatt tatatgataa
      661 gatgtctcaa ataataaaat agaaaagaga cataaaaatg attgacttgg caaagaaatt
      721 cttacacgaa aatatatatt ttgagttgta tcatcatcga gccatttata caaatgaaga
      781 tgctttggta ataaagaaag aacaaggatt tacaggtaca gagaccaaaa gcttattctt
      841 aaaagataaa aaagataata actacgtata tctgactttt actacgaaac aaacggattt
      901 caaagcatta gggaagatcg tcggtaaaag gctttctatt gtttcatcag aacgcatgga
      961 agaacaaacg ggacaaaagc agggcgctgt ttctccgttt ggttatgaaa aacagattcc
     1021 gattattatt gatgaagagc tcttatcaaa tgaaaaactt gtttttgcac caggtagacc
     1081 agatcaaacg atggtcatcc aaacggttga cttaaacaaa atcatccagc tacttgagat
     1141 gccgatattt ttgatgccgg caaacgaaaa aaacaattaa ccaagtaaaa tagtgcatac
     1201 ttggaggtga agcaacatga taagttatcc attattagaa agaaatgcaa cgattggggt
     1261 tactgcacca tcctcaggcg tatcagagga acaccattcg ttactcgaat cagctttaaa
     1321 gagaatggaa gaaaaatctt tccaagtagt tactggaaag acgctttgga cgcaagataa
     1381 ggaaaaatca ggtccagcaa gaaaacgagc agatgaattc aatcaaatga tgcaagatga
     1441 aaatatccaa atgatctttc cgccttgggg aggagaatta ttgattgaga ttctcgattt
     1501 tgtcgatttt gatgctctac aaccaaaatg gatagtaggt tattcagata ttagtacgtt
     1561 gctacttgct gtcacattaa aaacagggat agccactgca catggaacca accttattga
     1621 tttaagagga gagtacttgg atgaaacgac agcaatgtgg gaaagtgtac tgaaaacaag
     1681 agaaggagaa tcgattctac agcaaccttc tgaaaagtat caaaaagagt ggccccatga
     1741 gcattcatca tcttctgttt tccatctcac tgaaccaaca aattggcaat cagtcagcgg
     1801 agatgcagta aggatgaagg gacgattatt aggtggctgt attgatacga tcagtcattt
     1861 aatcggtacc ccttatggag atgtccaatc ttttaaaaga aagtataatg atggaaagcc
     1921 gatcttgtgg tttctagaaa attgtgagct atcgtcagtt gatttaagaa gatcacttgt
     1981 tcagatgaaa ctggcaggat ggtttgatgg cagctcgggg atcatgttcg gtagaagtgc
     2041 agcagaggat ttgttagatt ataaaatgaa ggatgtatat gaagatctgt caaaggaatt
     2101 aaaactaccg atcttgtatg atattgattg tggtcatgta ccgccacaac tgacattgat
     2161 caatggtgcc tatgcagaag tagcggtaga agatagaaaa gcgactgttt tgcaaacgtt
     2221 tatttaaaaa attttttcct gccttggagt ttatcaaaga ccaacaaatc taaatagaaa
     2281 agaaaatgaa agaagctgac agtattttgc cagcttcttt ttgtatgatc tattagaaat
     2341 tttaattatc cataactatc acattgatca gctgtttcac atcatttgga ttacctgtag
     2401 gaatagctac tacagatgca ttatcaataa tttcttgagt tgtttctcta atgaacgctg
     2461 gataagatgt ttgatctgca ggcatttgcc aatgtgaagg aactggataa ctagtgattg
     2521 tcccgtcatg gttacttgaa tacactaaaa ctccctggta actgtatttc ccagtgagca
     2581 taaccacatc tttcggatag actgcactcc ctgcatcata aggggcgata ggtgttcctg
     2641 caggataatg aataacatgt agttcgaaat cacccgatgt atctctaaaa gtattacctc
     2701 taattgctag ccaaacacgt gcgtactcaa tttgtagatc agaatatcct gctaacggat
     2761 ctattgactg agaagactgt gaggaagttg tgtctattgt tgaagaagaa atatttgtct
     2821 caactgtact actagatgta gaagaagtat tgggctctga gactttactg cttgttgaag
     2881 atgtattcgt ttcagtagaa gctgatttct gagtacttga tttttcagct gaactggttt
     2941 gtttgattga acttttgtct gtggtcgcag atggatcact attttcttta ctaggagacg
     3001 tacatcctac aaggaataaa gagaccaaca ataaaataaa acctaacttt ttcatataaa
     3061 atacctcatt atttttttaa taccagtata gcataatcct gaatttttga atccgtaaaa
     3121 ccttgtc
//
LOCUS       DADXTG010000192         3105 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.196, whole genome shotgun sequence.
ACCESSION   DADXTG010000192 DADXTG010000000
VERSION     DADXTG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.196"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWI57_002611"
     CDS             complement(14..706)
                     /locus_tag="IWI57_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBC2450705.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="IWI57_002612"
     CDS             complement(1143..1712)
                     /locus_tag="IWI57_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBC2450706.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWI57_002613"
     CDS             complement(1786..2109)
                     /locus_tag="IWI57_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450707.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWI57_002614"
     CDS             complement(2263..3075)
                     /locus_tag="IWI57_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450708.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          855 a          627 c          546 g         1077 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acata
//
LOCUS       DADXTG010000193         3091 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.198, whole genome shotgun sequence.
ACCESSION   DADXTG010000193 DADXTG010000000
VERSION     DADXTG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.198"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(405..1361)
                     /locus_tag="IWI57_002615"
     CDS             complement(405..1361)
                     /locus_tag="IWI57_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450709.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1470..1715)
                     /locus_tag="IWI57_002616"
                     /pseudo
     CDS             complement(1470..1715)
                     /locus_tag="IWI57_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1860..2282)
                     /locus_tag="IWI57_002617"
     CDS             complement(1860..2282)
                     /locus_tag="IWI57_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450710.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2462..2833)
                     /locus_tag="IWI57_002618"
     CDS             complement(2462..2833)
                     /locus_tag="IWI57_002618"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBC2450711.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT          934 a          685 c          510 g          962 t
ORIGIN      
        1 attgaatcac cacaacacaa tcaaaggagc attccgtatg cttgattttc attctataaa
       61 actactttta ggaatcaaag aaaatcacaa cggttcaaac taaaagcaag aatatccgaa
      121 gtaccattgt ttcatcgatc atgagaaaag cattcacctc tgtctgcaga tgttcttata
      181 tcgctctgtc agtcctatta ataaagtact gttttgtttt caaaaaaact attacgagta
      241 ctttttaaga gaatgccttg caaatattca tcacacaccg ttatataagt caaatagttt
      301 ctttcagtcg tcaagaaaag attctcctgt ccttggcatt atcagttaag tagcaactta
      361 attctcggac ttgttctact ctagcaaaat ttattttcat caacttacgt ccaaatgttt
      421 ttttgaataa ttacttcatc gcgggtctgg aagacttgcc tcattcgata agtcactagc
      481 ttttggcgtc cttcaggatt tctagtatac accaactgac atttgggcag cgacgattga
      541 tttaattcgt ttagaaaaag gttagcgatt tgcttatttc ggccaaagcg ttctggtacc
      601 gtcagaacta tctttttccc ttcgattaaa taccgcggat tctccagcgg tgctaaaagc
      661 tccctcatag cttcagtaaa aagctgtcct tctcgatagc cagcattctc aagatagcat
      721 ctaaattcct gctgatcagc cgctacgatt acttttctat cactaacttc accaaggcgc
      781 tgcaacgtcg tttgaacgca tatggccaag ctttttttgc gggcacattc cttttcccat
      841 agcttttgtc gcctacgaat ttggaaatat tccagcaagt tacctttaaa tatagctgac
      901 cagaagctta gaggctctat attttttact actatcggca atgaagtttc ttgtttttgc
      961 tttacctgat cgactttcct aacgacgatt ggcatagata ggtgccagcc atcagctaaa
     1021 gcagtactcc attgttcttt caaccgactt cgttcacaag aaaaatagaa catcgactga
     1081 gtataagttt ccatcgtggt ggtcgtatat tccagtggta aagcaaagcg atcgccacca
     1141 ctctcaattt ttggtgaaga ttcaaaggtc agacctgaaa tattgcccaa acgccgtagc
     1201 attcttttca aatcaggctg ttcagacaga ggaactcccg gtaatccaat cgctaaatgc
     1261 caaatgttcg ttactttatt ttgtgcacct aggcgtaagc cccgccctcg tagttgttgt
     1321 gtttgcacaa aagtggaagc ttgattagcc aagatcaaca gattaattgc aggacaatcc
     1381 cagccttctc ctagcaaact aacggttcct accaaaatat tcaacttctt ttcggttaat
     1441 aggcgagtaa ctgcttgcat caatgcttgg cggtttttgt catccaccgt caaataaaaa
     1501 taacccgaca tataaggatg ttcattcata cccgtcgcta gttctgccag ctcaacagtt
     1561 actagcattt tcggtaacag taagaactct ccacagatgg caccgatttg ctgagtgtta
     1621 cctaatagac gaacaacttc atcaaacaaa ggaaccacgc cataagctaa ggtaccatca
     1681 ctgcctgcta atacctcctt gcaaatacga tccatcaaaa tgaccgctgt cagttcttgc
     1741 ttcagctgaa cttcttcttt taacaaaatt tgacaaactg cttgcttttt ctcaggaatc
     1801 gcctgactac caacagtgtc tggatactct ggaaacaaat gcagcgactc atcaaacaat
     1861 catccagctt ttttcaattg ataaaaaata ggcaattccg gttgatcttc aaacatctgc
     1921 tgataaatag tgatcagctc ttttttacct aaagatggaa ctgaagcttt tttaccttgc
     1981 tgcttagaac tgcctaatag ctgccaatgt tcgggtatca attcatagtt atgatgattc
     2041 aagtaaatta atgcactagt gaacagctca aaattttcat aaatttcctt cgaatgctca
     2101 agcggatgct gaacaaattc taaagaaagc aaataatcca ccgtctcttg gctttccatt
     2161 aacgcttcca ccagctcatt ttgtttctct aaaaaggtaa aataatgctg agtagtttcc
     2221 tgattagccg gaatcaaata tagaaaatcc tgatgaggac atagaacctc ctcctttacc
     2281 aactcgggaa tgctaatttc ttcgtcaatc aggccatttt aactcaatat attgcttcca
     2341 ctccgtctga gagctatcaa tcggaggcgt ggctgtcaag gaaatcgtca acagattcgg
     2401 ttgttttttc ttcaagtcaa tcaacaattc accccagctt ttttttaaat gatgagcttc
     2461 atcaaaaaac taacagctcc agctgatcac cataagttgg ctcctccttc agtcggttgt
     2521 aaatggtctg atacgtttcc actgtaatat gactaggatg ttcaatcgcc ccagaaagat
     2581 agcaactatc tgctggattt actaagaaat attcctcgat tgttgttaac cactgtttct
     2641 tcaatattag ggaaggaacc aaaattaagg ttttacaatc caaacgtcgg atcatctcaa
     2701 ttcctaaaaa ggtcttgcca gcccctggtg gagccactag atggactgaa ttgttattta
     2761 aataagtagc aaagttagtc aatatacgct tctgatagct acgccactga attttttcat
     2821 cagatagcca catcccaggt ccccctctca cgtattttct ctatcataat ctaaatgaat
     2881 acagcaaaaa aaagatttct tcaagaatat gtgtttttat agtaatattt tacttactct
     2941 aaactcaaat gaaaaggtaa aaaaactggt agtacaatct tttaaattct cattgttttg
     3001 tacgttcttt actatcaaaa aaacaaaaaa aacgcgtttc tacgcgtgac caaaattttt
     3061 agctctataa tttctaagac tgatagaaaa t
//
LOCUS       DADXTG010000194         3071 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.199, whole genome shotgun sequence.
ACCESSION   DADXTG010000194 DADXTG010000000
VERSION     DADXTG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.199"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(334..696)
                     /locus_tag="IWI57_002619"
     CDS             complement(334..696)
                     /locus_tag="IWI57_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450712.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="IWI57_002620"
     CDS             complement(770..1150)
                     /locus_tag="IWI57_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBC2450713.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="IWI57_002621"
     CDS             1214..2407
                     /locus_tag="IWI57_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBC2450714.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="IWI57_002622"
     CDS             complement(2484..3068)
                     /locus_tag="IWI57_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBC2450715.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          959 a          587 c          503 g         1022 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctatctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat t
//
LOCUS       DADXTG010000195         3035 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.200, whole genome shotgun sequence.
ACCESSION   DADXTG010000195 DADXTG010000000
VERSION     DADXTG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.200"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..1668
                     /gene="alsS"
                     /locus_tag="IWI57_002623"
     CDS             4..1668
                     /gene="alsS"
                     /locus_tag="IWI57_002623"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBC2450716.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1681..2391
                     /gene="budA"
                     /locus_tag="IWI57_002624"
     CDS             1681..2391
                     /gene="budA"
                     /locus_tag="IWI57_002624"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBC2450717.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2521..3018
                     /locus_tag="IWI57_002625"
     CDS             2521..3018
                     /locus_tag="IWI57_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBC2450718.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1008 a          507 c          686 g          834 t
ORIGIN      
        1 tcaatggacg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt gattaatcat
       61 catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt taatgaatta
      121 gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc atttatggca
      181 caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag tggtcctggt
      241 gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc agttttagca
      301 attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag catggataat
      361 gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc agaaacaatt
      421 tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg cgcgagcttt
      481 atcagtatcc cgcaagatgt agtggatgca cctgtccaag ggaatgtcat caagccattg
      541 agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc tgaacgcatt
      601 agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga aaaagaaacg
      661 ttagccatca ggcaattagt tggaaagaca gcattgcctg ttgtagagac attccaagca
      721 gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg tttatttaga
      781 aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgctat tggctatgat
      841 ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat cattgtgatc
      901 gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt gatcggtgat
      961 atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt gtcagaagat
     1021 gcaaaagaat atttagcatc tcttcaagca gaattaacgg agcgggacat tgttcaatca
     1081 aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca gtcaaaagta
     1141 acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat ggcacgccac
     1201 tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac gcttggtgtt
     1261 gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacaaat cgtatcagtt
     1321 tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt acgtaaaaaa
     1381 ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga gttccaagaa
     1441 aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt tgtgaaatat
     1501 gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt agaaaaagct
     1561 ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat tgattaccgt
     1621 gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg aagtgtcgac
     1681 atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc tggcttgatg
     1741 gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt gggaacattg
     1801 catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg aagatcagat
     1861 ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat tacagatttt
     1921 acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt gaaaaaagac
     1981 atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac tggattattc
     2041 aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag actagcaaaa
     2101 atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt agtcgggttc
     2161 tttacgcctg aattattcca aggagttgct gcagcgggat ttcacttgca ttttatcgat
     2221 gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg aaaagttgag
     2281 attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac ttttgttcac
     2341 gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata gaaaaagaca
     2401 gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga ggaaacctag
     2461 cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc ggtttttatc
     2521 atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca gcaacaagga
     2581 ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc attgttgaat
     2641 gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa tgaattgatt
     2701 ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa aggagtttgt
     2761 ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac ccatgcaaat
     2821 tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt aaaagaaggt
     2881 aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga tgaaatcgat
     2941 caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat aaatgctttt
     3001 tcatttatta ataactaaaa tcgtctattg aaaca
//
LOCUS       DADXTG010000196         3026 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.201, whole genome shotgun sequence.
ACCESSION   DADXTG010000196 DADXTG010000000
VERSION     DADXTG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.201"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..325
                     /locus_tag="IWI57_002626"
     CDS             <1..325
                     /locus_tag="IWI57_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HBC2450719.1"
                     /translation="EKYADVEENQTALPSRFDELIEQKRELKRTKKEIESSIRQVDNE
                     IISELGKREASIGITQRNIISWKLVRTRRMNTKKLAEKYPDVANDEEIYNVTESRRLT
                     EKEIK"
     gene            327..1217
                     /locus_tag="IWI57_002627"
     CDS             327..1217
                     /locus_tag="IWI57_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBC2450720.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            1260..2060
                     /locus_tag="IWI57_002628"
     CDS             1260..2060
                     /locus_tag="IWI57_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBC2450721.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            2075..2926
                     /locus_tag="IWI57_002629"
     CDS             2075..2926
                     /locus_tag="IWI57_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2450722.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
BASE COUNT         1142 a          501 c          664 g          719 t
ORIGIN      
        1 agaaaaatat gcagatgtag aagaaaatca aacagctcta ccatcacgtt ttgatgaact
       61 tatcgagcaa aaaagagaac tcaagcggac gaaaaaagaa attgaatcat ctattcgtca
      121 agtagacaac gagatcatca gtgagctagg taaacgtgaa gccagtatcg gtatcacaca
      181 aagaaacatt atcagctgga aacttgttcg tacaagacgg atgaacacga agaaactagc
      241 agagaaatat ccagatgtcg caaatgatga agagatttat aacgttactg aatcaagaag
      301 gctaaccgaa aaggagatca aataatatgg caacaaatga atcgttaaaa aatcaattgg
      361 cagaaaagcc acagaaacaa gttgcaccag gacagttagg gcttaaagct ctaatgaata
      421 caccaacaat gagaaagaaa tttgaagaag tacttcatga caatgctaat gcttttatgt
      481 cgaatgttat gactcttgta tctaatgaca gttatcttgc agatagtgaa ccgatgtcta
      541 tcatgagtgg tgcgttaact gctgcaacat taaatcttgg gctagataag aatttaggtt
      601 atgcatattt agttccattc aatagtaaaa acaagcaaac aggaaaatgg gaaaagaaag
      661 ctcaattcat gcttggctat aaaggatata tccaattagc ccaacgatca ggtaaataca
      721 aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa aagctggaac cgactgacag
      781 aagagtttga gtttgatcca aatggtagaa cgtctgatga agtcattgga tatgttggct
      841 atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg gaccaaacaa gaaatcgaag
      901 ctcatcggat tgctaacaat aaagatcgcg ataagacaaa gttaagtggt gtgtgggcat
      961 ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa tcttctttct aaatggggaa
     1021 tcttgtccat cgaaatgcaa gaagccacca catcggatga gagagtccaa agggttcaag
     1081 aagacggcag cattattgct gaaacagaag ttgaagaaga tattcctgaa agaaaagaag
     1141 cagaggttat atctgaagaa aatgaagatg tacaaactgg attatttgat gcatctaatc
     1201 cgccgttaaa caaataatga gggagctttc tcccttgctt ttctagagga gaaatacgaa
     1261 tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa ttcattcgtt tggaccaatc
     1321 cttatatgta taaattatgg agcttgtgtt tgatgaaagc gagtcatgag aatcgcaaga
     1381 tactttttaa tggaaaagag atacaagtga acagcggaga attcgtcaca gggcgcgatg
     1441 ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca tcaagtgaac agcggttctg
     1501 tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt gaacatcaaa tcaactacga
     1561 aatacagcgt tatatcaata aaaaactggt ctttgtacca aagcagtgaa caacaaatga
     1621 acatcaaacg cacaacaagt gaacaacaag tgcacacaaa caagaatgaa aagaattata
     1681 agaatgaaaa gaataataaa acatcatcgt cgcaaccacg caaaaagcgt gtttacgaca
     1741 ccgactcagt ttactacatt ctcgcggagg agttattcaa acagatttgt cagaatcagg
     1801 aaatcaaaaa gccgaatctg caaagttggg cagatgacat tcggaaaatg atcgagatcg
     1861 acaagcggac cgaaaaccag gtacggggaa tgattgaatg gagtcagcac aatgtgtttt
     1921 gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa atacgacaca atggcagcgc
     1981 aagcaaatcg tgattataaa acaaaacaaa ctaaaacgct tgaatacgag aaatttagca
     2041 cagatgagtt gcctatttga gaggaggcgt aggcatggaa actgttgggg aaatcatgga
     2101 aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt cctgaatgcg gacagccttt
     2161 gtatggatgg cgtacgaaga accctgatgg ctcagaacgt tgtaagccaa cctgtatgca
     2221 atgtgggtat aaagcattgc gagttcagga agacttacaa acagaacgaa tttacaacga
     2281 gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt tctgttgtgc ctaatcaagc
     2341 gttgtttgat tgcacattgc agaattatca aattgtcgat caagaaacaa gacaagcggt
     2401 tgaagtaacc aaacgctttg ttaattcagt cttgttagga aatccaagtc accttgtttt
     2461 aactggaaaa caaggaacag gtaaaagtca cctagcgatg gcagcggctt gggaagtctt
     2521 gaagcgatca aactacgata aaaaaatctt atttatcggg ttacaggaaa tgctggatca
     2581 aatcaaattt tcgtacaaca atcctgaact cagaaaaacg attgagggat cgttgattgc
     2641 agatatcaaa acagcggatt tagtcatcat tgatgatatc ggttcagaac taggaaaaga
     2701 tgcatcagat agtcgagcgt ttggcataaa cacgctaaat tcgttcttgg atgcacgaca
     2761 gaacctagca acgattatca caacgaactt gcttggtgaa gaactgaaaa aagcttacgg
     2821 tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga tttacgatgg tattttctca
     2881 aacagcagac aagcgcataa aaccagtcaa aggagcgatc gcatgaattt gactaaacaa
     2941 gaaaaaagaa tctgtcgtca gataatgttg caaattatag gtgatcgtga catggacgaa
     3001 ttatctcaac aagagagtcg aatggt
//
LOCUS       DADXTG010000197         3015 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.202, whole genome shotgun sequence.
ACCESSION   DADXTG010000197 DADXTG010000000
VERSION     DADXTG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.202"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(156..518)
                     /locus_tag="IWI57_002630"
     CDS             complement(156..518)
                     /locus_tag="IWI57_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBC2450723.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            741..1493
                     /locus_tag="IWI57_002631"
     CDS             741..1493
                     /locus_tag="IWI57_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBC2450724.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1633..2556)
                     /locus_tag="IWI57_002632"
     CDS             complement(1633..2556)
                     /locus_tag="IWI57_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2450725.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2571..2843)
                     /locus_tag="IWI57_002633"
     CDS             complement(2571..2843)
                     /locus_tag="IWI57_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450726.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          982 a          573 c          494 g          966 t
ORIGIN      
        1 aaaatatcac acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa
       61 acaaaaatgg cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg
      121 gagcaggtta ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt
      181 tgttctatca ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc
      241 tcctgtttca ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga
      301 atcgcatagt cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc
      361 tttttatcat atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa
      421 taattaatag ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata
      481 tttaaattac tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag
      541 tcgatttaga tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta
      601 caattgaacc tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt
      661 tcgtgagaaa aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac
      721 atttgtcagg aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag
      781 tcagtccgag aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca
      841 attcttcagg ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat
      901 tttatcgaac aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt
      961 ttgaccacca gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa
     1021 tcgataccct cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa
     1081 tgagtgacaa agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat
     1141 cctatggaca agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa
     1201 aatggatgaa tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt
     1261 tatttgatct cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct
     1321 tccaagctca taagaagtgg ttctcttaca gctggcctga atatcagcca aaagcgcata
     1381 aaggactagg aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa
     1441 gtggtcatgg tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta
     1501 catgatccaa caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag
     1561 ttagattttt atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta
     1621 ctttaaatat attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc
     1681 tgtaagaaac aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg
     1741 gaaaccaaga agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc
     1801 ttgcagatag ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc
     1861 aactccaagc tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc
     1921 ccaaaaaaca agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat
     1981 ttttgagtta taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc
     2041 ttgctgcttc aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca
     2101 gcttgcctgt tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc
     2161 caataaatca tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc
     2221 tgcaaattct tcaagatacc ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt
     2281 cactttgtaa tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata
     2341 cggatgattg gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg
     2401 tctggcattt gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct
     2461 tttttcctca tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata
     2521 acctgtgagc tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa
     2581 agattttctt gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt
     2641 ccttcatgcg aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg
     2701 atttcattca ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac
     2761 gttaatacgg atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct
     2821 gttatctcta cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat
     2881 gaaacgcgtc tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat
     2941 tattaccaaa gtctcttatt ttaaattgaa tacattataa tgatactgta aatatatctt
     3001 tattattagt gaatg
//
LOCUS       DADXTG010000198         2977 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.203, whole genome shotgun sequence.
ACCESSION   DADXTG010000198 DADXTG010000000
VERSION     DADXTG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.203"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(194..808)
                     /locus_tag="IWI57_002634"
     CDS             complement(194..808)
                     /locus_tag="IWI57_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBC2450727.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1414..1791)
                     /locus_tag="IWI57_002635"
     CDS             complement(1414..1791)
                     /locus_tag="IWI57_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450728.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2091..2972)
                     /gene="galU"
                     /locus_tag="IWI57_002636"
     CDS             complement(2091..2972)
                     /gene="galU"
                     /locus_tag="IWI57_002636"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBC2450729.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          902 a          624 c          468 g          983 t
ORIGIN      
        1 tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga
       61 tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt
      121 tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc
      181 aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca
      241 aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta
      301 gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag
      361 caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa
      421 atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga
      481 ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa
      541 gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat
      601 tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac
      661 cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga
      721 tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt
      781 gataatagtc aaaataacat aaactcattt atctctcccg cctagctata cttgacatga
      841 atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac
      901 atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg
      961 acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc
     1021 accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc
     1081 ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag
     1141 ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa
     1201 gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa
     1261 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
     1321 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
     1381 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
     1441 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
     1501 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
     1561 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
     1621 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
     1681 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
     1741 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
     1801 acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg
     1861 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
     1921 tagcagaata ttttctgact ctattcacga aagtcttttt ctatctttcg ttttccacga
     1981 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
     2041 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
     2101 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
     2161 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
     2221 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
     2281 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
     2341 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
     2401 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
     2461 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
     2521 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
     2581 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
     2641 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
     2701 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
     2761 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
     2821 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
     2881 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
     2941 gcggcaggaa tgactgcttt tttgaccttc acttgct
//
LOCUS       DADXTG010000199         2945 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.204, whole genome shotgun sequence.
ACCESSION   DADXTG010000199 DADXTG010000000
VERSION     DADXTG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.204"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            128..565
                     /locus_tag="IWI57_002637"
                     /pseudo
     CDS             128..565
                     /locus_tag="IWI57_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(642..2189)
                     /locus_tag="IWI57_002638"
     CDS             complement(642..2189)
                     /locus_tag="IWI57_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBC2450730.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(2291..2644)
                     /gene="tnpB"
                     /locus_tag="IWI57_002639"
     CDS             complement(2291..2644)
                     /gene="tnpB"
                     /locus_tag="IWI57_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBC2450731.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2634..2828)
                     /locus_tag="IWI57_002640"
     CDS             complement(2634..2828)
                     /locus_tag="IWI57_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450732.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          863 a          604 c          539 g          939 t
ORIGIN      
        1 ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac cacaacattg
       61 aaaaatctgg ttttatcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt
      121 aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg aacaacgcca
      181 gattgaagaa atgaaaaaat tcggtgcagt gagaattttc atcgagaaac aatcgggcgc
      241 aacgattact catcgacctg ttttccaaga agcattggat ttcgtccgag atcaagacat
      301 ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca tcgcttcagt
      361 caattaccta aagaagaaaa acgtacagct aatcatcacc agtctaccga tcatggctga
      421 agcaattggc aaccctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc
      481 catgatcgct gaacaagagc gtaccgaatc taaacgacgc caggcgcaag gcattaaaat
      541 tgcgaaagca aatggagttt ataaagtaag cgccccatag aacagtacct gtacgtcaaa
      601 taacccgacc tatagaatag aatcgggaat gtttggcgtt tttagttacg agaaaaatgt
      661 tgttgtacat ccttgttcca aggcaacagt tgatctaaag tttctgctga aagtacggga
      721 agatttggta tcttatcaaa tagaaactct agatacttta caggattcaa tccgttaagt
      781 tctgctgttt tatatacact cagaatgatt cctgaagttc gtgctccttc aagacttgtg
      841 gaatgcaacc aatttttccg tcctatcacc agctctttga tatttcgctc cgcacgatta
      901 tttgtgagtt ctaaacgacc atcttcaaaa ataggttcaa aaccacttcg tagtttacac
      961 gcatattgaa cggctgcatc caacttggat tttgcaacag gatctatcgt tgccatccac
     1021 tcataaaaag caataaataa tggacgcagt tcttgttgcc gacagcttaa tcgcttctca
     1081 ggagaaaagt ctttccattt cttttctagg acaaaccatt tatccaattg tttgactgct
     1141 tgagctgcgg gaatatccgt gtttttcttt ccttttggaa ttgcttcgaa aaatttccgc
     1201 cgaacatgcg cgaaacaata gacaagtcgt acactttcca cggcattata cccgctatag
     1261 ccgtcacagt gtaaataccc tgtaaactcc ttgagaaaag ttttcggaac atctccagca
     1321 cgactttcgt cgtggtgata ataaacgatg ggatgctgac tgtcttttcc tgtacaaaac
     1381 tgccagtaat aggtatctgt tttggcactt tcaatcacac gataacttgt ttcatccccg
     1441 tgtagaatct cttcttgatt aagatagact cttaatcgtt ctcctaaagg gtctaacgcg
     1501 ttttgcactg ctttaatatg ccaattggtg atattatctc tcgaaatatc caacccatgt
     1561 gtttgtttcc aatagttctc ttgccgatag gcaggaactt tctgaccaaa tttcatccgg
     1621 atcgtttccg ccactaaact attggaacct aagctattag aaatcaaagg tttaggaacg
     1681 atcgcttttt taaaagaaga aagtccttct ttttcgcagc ttttacaaca ataggtatgt
     1741 tgataatggg tgagacaatc caacatagcc tgatggaaac gaatttcttc tcgtactttt
     1801 gtccgtccga tatctttcat ctgtgaaccg caatgttcac aaaattgttc ttccaaagga
     1861 aggagatgtt ctttctctgt tataggtaag tgtttgattt tgtctgcttt cgttcctaca
     1921 actcgttgat gtccttttac tggaataatg gtggcagctg cttctggaac acttacagaa
     1981 atttcttcat cactaaaaag attcaattgc ccattcgttt cctcaaagag agtctcttta
     2041 gagcgtccaa ataatttgtt ggttaaataa ttgacctttt cattcaatag ttggatttca
     2101 gttgtaagat tctgaatggt ttgttgttgt gcttgattgg ttttatgggc ttcttgaagc
     2161 aactgaagca acgtatctgt agtttccaaa atttttcacc accttgaatc gaactgattt
     2221 tgttacagaa taccacaaga aacgcacaag aaataggggt tcaacacatt ttttgtgtta
     2281 accccccagc ttaaaaaact cctttttttc ctggcaaaat agctcgaggt tgatcgatac
     2341 ttaatccttc taacaaccat cgaacctgtt ggttcgttaa ggttcgaact tcgtctttcg
     2401 tccgaggcca ttttaacttg ccgttttcta tccgcttata acagagaaga aaaccatcac
     2461 cgtcccaata gagaagtttg aaacggtctt gacggttccc gcaaaataaa aaaacggcat
     2521 cttcgtaaag ctcgagttga tactcttgtt gaataagact ggcaagacca tcaattcctt
     2581 ttcgcaaatc tgtttttccg cacacaatat aaatatgttt ggtttgaagt aaatcataaa
     2641 gcattggaca acagttcttt caaaatagct tgaagaatat aattgttaat accgttatag
     2701 atcaaaactt cagaaccatt ctctcttttg atgcgcatcg ctaacttggc agccgcgggt
     2761 ttaccttttt ctggtgcact cttttcgttt cctaattgga caggaacaat ctcatttaat
     2821 ttatacatag aaaacctcca gacttcttga tagctcaagt atatcttgag gtgcatttgt
     2881 tagtaaggta ctgttctatg gggcgcttac tttataaagg tcgtccaaaa ctctatagtg
     2941 cagat
//
LOCUS       DADXTG010000200         2845 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.205, whole genome shotgun sequence.
ACCESSION   DADXTG010000200 DADXTG010000000
VERSION     DADXTG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.205"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..160)
                     /locus_tag="IWI57_002641"
     CDS             complement(<1..160)
                     /locus_tag="IWI57_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2450733.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
     gene            426..770
                     /locus_tag="IWI57_002642"
     CDS             426..770
                     /locus_tag="IWI57_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBC2450734.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            771..1190
                     /locus_tag="IWI57_002643"
     CDS             771..1190
                     /locus_tag="IWI57_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBC2450735.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1282..1632)
                     /locus_tag="IWI57_002644"
     CDS             complement(1282..1632)
                     /locus_tag="IWI57_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450736.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1598..2818)
                     /locus_tag="IWI57_002645"
     CDS             complement(1598..2818)
                     /locus_tag="IWI57_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBC2450737.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1036 a          510 c          558 g          741 t
ORIGIN      
        1 attctaaggc tgattttcct tgcttgaaga acagatatac tttatggatc gtttgtcttg
       61 aacgattcaa gcaatgggca gttttagcaa cagattgatt cattttgaaa taagactcta
      121 ttattacaag ttcatccgtt gtaagatggg tataggtcat ttgtactcac tccttataat
      181 tctttggtcg gaactatttt gagtgtagca caaatgactt ttttagttgt ctagcttaat
      241 tttacaatcg gcgaataaaa aaagcgttag caactatgct taaaaagcat atcgactgaa
      301 taaactaagc attggacagg ctgaaagact acatttaaaa tgaaatgcga agaaacaata
      361 gaaaataaag ttgccttaga accatagttg aatcacctaa cagtcgtgaa gggagaataa
      421 gagaaatggc agaaaaacaa accgcaggac gagatcaatt aggagatttt gcaccaaaat
      481 ttgcagaatt gaatgatgat gttttatttg gacaagtatg gtccagagaa gaattatctg
      541 ctcatcagcg tagtatcatc accatatctg cattaatttc aggaggaaat tttgaacaat
      601 tgccagctca tctgaagatt ggcaagcaaa atggcattac tagtgaagaa atagcagaaa
      661 tcattaccca tttagctttt tatgtaggat ggccaaaagc ttggtcagct tttagtttag
      721 caaaagaaat ctatttagaa gaaaatagcg aagataagaa agaggcgtaa atgacgacaa
      781 aacacgaaga aatacacaat ggcgtgattt ttccagcagg tgaaaaaaac gaagcttacg
      841 ctcaagtatt taccgggcaa agctatctga atatgttagt gaatgatccc gaagtttctg
      901 ttggtgtagg taatgtaact tttgaaccag gttgtcgcaa tcactggcat gtccataaag
      961 acggctatca aatcttactt gtgaccggag gagaaggctg gtatcaggaa gaaggaaagc
     1021 cagcaagacg attaatggct ggagatgtcg tcgtaacgca caaagggatt aaacattggc
     1081 atggtgcgac aaaagacagt tggtttgaac atttggcgat cactgctggt aattcagagt
     1141 ttttagaacc tgtaacagat gaagtgtatg aaggattgga aaatcaataa tgtcaagctt
     1201 gaacagctcc tgaaagtaga ttttaatcga actttcagga gctgcttttt tgattcagtc
     1261 ttttataaat agaaaatagg gttaataata ggaatgagaa gcacgtcgaa agatcttaat
     1321 attgataatc ggctgggcat acatgatttc cacgtcatca atcaaactac ctaacagatg
     1381 ataatcttcc cgatcatgtc ggggattacc taatagttct tgataataga tttccatact
     1441 agggtcccga ggattgttca gcaaatcagc aagtacatct aaatcttctg gctgagtttc
     1501 tgccgtaccc tgtatctcta tgaaaagccc ttcctcagca aattctactc caatacgtat
     1561 ttcagttatt tcatgggaat ataaataagt aagaagctca tcgatgattc tttttgataa
     1621 ttctttttcc aagtgtcttt ttttcctttc tgacagcggt aaagttatct agtatcggta
     1681 caaggatacc tgcaacgaaa ccagttgaaa aaccactgtt atatagattt agtcctccgt
     1741 gcaaagtgct gacgttatgg acaagagcaa tatgtagtac accagctatt acaccgtaaa
     1801 aactgccgta aaaaccgctt atgggcgcga gacctgtccc aaacacagct gcaacaagca
     1861 tgccagtact ggtgatctca tgaaaagttc caaacaaaga agcaataaat attccgacta
     1921 gtatcggaaa actgtttttt aaatgacaac caaaagcact gaaacccacc gcagataaaa
     1981 tagcaccgat gacaggtcca tttaactgac cgccaaccag caaaacataa gaagacagca
     2041 tcagaccaac taagcctatg ttgatcaaag tagcaccaat accagaatta gcaatgaaat
     2101 cagttgtcaa ttttccagat gaatcgaaaa tttctcgaat ccccaaaagc cgaaaggaat
     2161 tataatagaa accaattccg actgtcagcg aaaaaaagcc aaaaataatc caaatgagag
     2221 gaaagtgata ttcattgaaa accaatgtat tttcgacgat tgaatagctg aataacctca
     2281 aaacagcagt aaaagtcatc gcaatcaacc cagaggtgaa tccaatatta taaagtgtga
     2341 atccacgatg gaaaactaaa gtttgagcag ctaagggcgg aagaagtaga ccaattgcta
     2401 tgccagctaa attccccaat aaaataccta tcagtaaagg aaaacgcata ccaaaagtga
     2461 tataactaac gaccggtgat aatgcgctac caaacagtcc gatcatgatg tactgagaaa
     2521 aaggtttatt gacaaataca gcgtatagat acacacctaa aatgattgag atagaattat
     2581 aaaggttttt gccaaaaaaa gagaaaccgg taactgttag aatcgaagca aataagggac
     2641 cgtttaaaat caccttgtgc cgataagcta aaagcacaga aagtaaggtc aaaatcccta
     2701 catttatgaa tgcgcttccg aaaccgccta aagcaaaata atctgtcaaa agattgcttg
     2761 gcgaggtaag aatagttagc aatccactcc aattatcagt aggagtatta gaaagcaagc
     2821 caataaaaag aaaaaaaaca cctgt
//
LOCUS       DADXTG010000201         2744 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.206, whole genome shotgun sequence.
ACCESSION   DADXTG010000201 DADXTG010000000
VERSION     DADXTG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.206"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..578
                     /locus_tag="IWI57_002646"
     CDS             3..578
                     /locus_tag="IWI57_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBC2450738.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            578..1018
                     /locus_tag="IWI57_002647"
     CDS             578..1018
                     /locus_tag="IWI57_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBC2450739.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            1034..1735
                     /locus_tag="IWI57_002648"
     CDS             1034..1735
                     /locus_tag="IWI57_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBC2450740.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(2223..2648)
                     /locus_tag="IWI57_002649"
     CDS             complement(2223..2648)
                     /locus_tag="IWI57_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450741.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT          909 a          448 c          548 g          839 t
ORIGIN      
        1 ttatgaaact tactgttttt tgcggttccc gttttggtaa taaagaaagt tacaaattta
       61 ttgcgcaaac gttaggaaaa tacatggcac aagaagaaat cgaactggtt tacggtggtt
      121 catactccgg gattatggga gtgattagta aaacagtact ggaaaataac ggaaaagtca
      181 caggtatcta tccgaatggg ctttttgaaa ctgaattgcc tggaaaagat gttactacat
      241 ttattcctac agaaacgatc gatgaaagaa aggtattatt atttgaaaag ggagatgctg
      301 tcctcgtttt ccctggaggt ctaggtacat tggaggaatt ctcacaactt ctttcttgga
      361 tagcaatcgg tctgacgccc gacaagccaa tcggaatatt gaatattggc ggttattaca
      421 gcggcttgca acaattatta gaaacttttg caaaagagga ttttatggat aaaaaatggt
      481 tggatcaagt aatcttttcc aacaatccat tggaactggt caatgaatta cgagcagtat
      541 ctattaaaaa tcagttatta ttgaaggagg caaaataatg agtcaaatat atttagcagg
      601 accatttttt tcagaggagc aaatcgatcg agtaagtcgt atcgaaaaag cgttggagga
      661 aaacaaaaca gtcacaagtt tctactctcc acgtcatcac caagaaagca attatgaact
      721 attcagcgct ggatgggcgc aggaagtata cgaaaaagat atggaagaac tgacaaatgc
      781 agaatttgtt gttgctatcc ttgactttga gcatcaaaca atcgatccag gaacggctta
      841 cgaattaggt gtggcaacta tgttgaaaaa accgatgatc attgtacaag aagagacggt
      901 acctacgaac ttgatgatta cacaaagtct ccatacgtat ttaaaatcag accaagctgt
      961 acgtgaatat gattttgaaa cattgcctgt tgaaacttac gtaggcgagt acttatagaa
     1021 gaggaagggt tgtatgtcag tgattttgat ttcaggaacg attggtgcag gtaagagcag
     1081 tttgacggat atgcttgcaa aagagattga ttctaaacca ttttatgaaa atgtcgaaga
     1141 taacgaagta ttgccgctct tttattcaaa tcctgagcag tacgcttttt tattgcaaat
     1201 cttctttttg aacaagcgat ttttagcaat gaaaaatgct ttagtgaacg atgacaatgt
     1261 gctagaccga tccatctatg aagatagcct gttgttccat ctaaacgctg atttaggtcg
     1321 ggtaacggat atcgaagtac agcagtacga tagcttatta gatacgatgc taaacgagtt
     1381 agatgatgtt gctccaaaaa aacggcctga tttgatggtc catatcaaag tttctttgga
     1441 cacgatgcta gaacgtatca aaaaacgtgg gcgtgactat gaacagctag aaagcgatga
     1501 gacgttatat acctattatg aaacgttgaa cacccgctat aatcaatggt atgaagattt
     1561 tgatgtatgt cctaaaatac aagtggatgg ggataaattc gattttgtag aaaatccaga
     1621 agatgcaaaa aaggtgatcc gtcagatcca agaaaaatta gctgaattgg aaggaaagcc
     1681 tgctggaaaa tattttacgg ataaccagca tgcaaaaggt caagaattag gctaaaaata
     1741 aaaaaccaaa cgacgggaaa gaaaaattac tacgtgatgt agaataagcc tttctatcgt
     1801 ttggttattt tatattcaaa tatttgaaat aaataaatct ttactctttg atttgtaaac
     1861 tgaaatgatc cactgtttgt tttactgatg aagcttgatc aaaggaagaa gctgagtttg
     1921 aagttcataa aaatcagcca tagtgagttt tgcttggatt tgtttatctt cttctacttt
     1981 ttacataatt ttgaacagga taagattcaa atgttttaaa tgaatctttg actagtttgt
     2041 gacagttgtc gcatatgctc aagcttcctt ggtcattaaa ttctgtatgg atatttttgt
     2101 tgttattaca tgtacaataa tcaggactat cgatcatcca tttcgtctcc tttacaatca
     2161 tcttgcttct ttactatagc agaaaagaag gggagaaaca aaagaagatc caacagacgg
     2221 cactatagac tgtagatgtt gccatcctca tccttcaaac ggtaattacc gatttttgta
     2281 aatatgactg ttgtttcttt tttattttct tttcctgaaa taatatattc gtcatctttg
     2341 gtagtgatct tgatatctgt cagcactgtt ttttcttcag atgattcaga agaatcagtt
     2401 ccttgtgtct gatacgtgcg tgtccattcc atttttttat ctgtaaatgt caactgtccc
     2461 gcggataaat ccatatcttt acgtactttt agcgtatata cactgttggt ggtatctgct
     2521 ttttccgctt ttaagacaga agaatcggtt gtttcgtccg aggattggtc agagccgcca
     2581 aacaaatcac agccagtcaa tgtcaaaagt cccgcagaca aaataaatag tacgattaat
     2641 ttcttcaaaa tacttccccc atttgatagg ataaagaaat tataccattt gaatgtaaaa
     2701 cttcaaacgg atggaagaga aaaacgcaca aaacacccac aaat
//
LOCUS       DADXTG010000202         2667 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.207, whole genome shotgun sequence.
ACCESSION   DADXTG010000202 DADXTG010000000
VERSION     DADXTG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.207"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            116..1201
                     /locus_tag="IWI57_002650"
     CDS             116..1201
                     /locus_tag="IWI57_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HBC2450742.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            1224..2039
                     /locus_tag="IWI57_002651"
     CDS             1224..2039
                     /locus_tag="IWI57_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450743.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            2023..>2667
                     /locus_tag="IWI57_002652"
     CDS             2023..>2667
                     /locus_tag="IWI57_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HBC2450744.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVL"
BASE COUNT          903 a          358 c          537 g          869 t
ORIGIN      
        1 aagttcgaaa atgaacttga tttctatgcg aagttggatt caaaaaaatg gatggatatt
       61 tatgctaatt atacaccttt tagtcagtga ttatatggaa aggatgaggt gaataatgaa
      121 aatcggaata gtgactattg ttaatggggc aagttttggt aacagattac aaaattatgc
      181 gactcaaaaa gtgttggaaa ataacggcta tacagttgag acaattaata attgttttga
      241 caaagaaagt accctatcca ttgttatcaa aaacgctatt tatacattat ttaagtttat
      301 accgcatttc aaaaagtcaa aaagtatctg gaaaagaatg tatacatttt atcaatggga
      361 caagcagtat atagattatt ctcatcgtaa aattgatgca tctaaaaaaa acagctttgt
      421 gagtataaag aatgattatg actatttctt ggttggtagc gatcaaattt ggaatccaaa
      481 atatgcagcc aataagggat ttgaactttt atcatttgtt ccaaatgaaa aaagagtttc
      541 attatcaacc agtttgggag ttccatctat accaagtgat ttagaaaaat tatatgtgac
      601 agagtggaat aagtttaaat caatatcggt acgtgaaaat caagcggcaa aaataattga
      661 ggacttgaca ggcacaactg cggctgttca cattgaccca actttaatgt tggagcgttc
      721 ggaatgggaa gcctttcaaa aaaaaccaga caatattgat gaaaattaca tcttgtcgtt
      781 tttcttaact aatccgccta aggatactgt tgatgtatta gataaggtcg ctaaagattt
      841 gaaaataggt gttctcacct tagatgcgtc ttgggaatat tttcaagata ttactccgaa
      901 tcattttgtt tatttgattt cacatgcaag aatgatttat acagattcat tccactgcac
      961 agttttttca ttgctatttg gaaaaccggt tgtggtattt gaccgttttg atagtgaaaa
     1021 aatgaattct aggttaagta cattgttaaa taaagtaaat aaagcacatt tttgggtagg
     1081 aaaaaaagct gatgaagcaa tgattcgtgc aatagcatct gaacctgaat atgacataga
     1141 taagctgatg gtatcagaga gggaatgtgt aaaagcttat cttaaagagt atttacagta
     1201 aaattttgaa atgggatgaa aatatggagc atttaagtga ttgttttggt aaaatgaaga
     1261 ttgttacgac ttcatgcgca agagaatatt ttaaggatga tttggataaa tcagagtatt
     1321 catgttttgt tgattctcga gcagctatgt ttttcgcaat gggacaagct ttagctaaaa
     1381 gaaggaatgt agttgtaatg cttgctgatg aattgacatc gtgttatacg gcactaacag
     1441 aatgtgcatt tcaacaagtt gatttaattg tgtttgcttt tcagacagtc agacatgaaa
     1501 ttgatttagc atctttgaag aattgtggtt gcaatctaat taactgtagc gcccaagacg
     1561 atattgatag agttgtgtcc aacactaagg ggttaaatca gacaacagta attaaaatca
     1621 atatgaattg tgagtcggat aaagatcata tatgtgagta tatagacctg ccagacatac
     1681 ctgttttttt ccacggtagc ttatactcac ctgcaagtaa aaagaaatat gagcagtatg
     1741 gacaggtatc gaagtattta ggctatctcg tggggggaaa agatgcaata ctcataattt
     1801 ctcacaatga gtttttaaac gatatcagtg tatggattaa ttattattct tttggagaaa
     1861 ttcaacccaa aattattata tttgacagtc cgaatgacta tcaatataaa tggctgtgtg
     1921 acttagaatg cagtgtagct cattcaacca accgattttt gtctgaggat ttagccttat
     1981 tgcaaaataa cggaatttcc attgtgttaa gaggtgataa ttatgatatc gtcagttaaa
     2041 aatgttcaaa ttttagtatc tttattaaaa cagcataata ttaaagatat agttatctcg
     2101 ccaggtagta gagattttca attggtgcat agtgtagaaa cagacagttt ttttagaacc
     2161 tatacggttg tagatgagcg cagtgctgcc ttttttgcta atggactttc tatggcgatt
     2221 gataagcccg tgtgtgtatg ttgtacttct tcaactgcct cttcaaatta tatggcggca
     2281 gctaaagatg cttttgctaa tggcatccaa cttgtacttc ttactgctga tagagattac
     2341 cgccgcttat atcagatgga agatcagatg attgatcagg tagatatgta tggggcttat
     2401 gtgcgttcta acgttgatat tcctattgtt aaggattctg atgatgaatg gtatgctacc
     2461 agaaaaataa atgaagcaat attggagtta aatcatggcg ttaagggtcc cgtgcagatt
     2521 aattatcaat taatggattt aagtgcgccg tttgttgaga ccatgccaga gtatcgtaaa
     2581 attacaagat atcaagagga tgatttactg tcatcagtag agatttttaa acagaaacta
     2641 ttatcaaaaa agagaataat ggtttta
//
LOCUS       DADXTG010000203         2656 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.208, whole genome shotgun sequence.
ACCESSION   DADXTG010000203 DADXTG010000000
VERSION     DADXTG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.208"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            38..1669
                     /locus_tag="IWI57_002653"
     CDS             38..1669
                     /locus_tag="IWI57_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBC2450745.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLIIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1672..>2656
                     /gene="uxuA"
                     /locus_tag="IWI57_002654"
     CDS             1672..>2656
                     /gene="uxuA"
                     /locus_tag="IWI57_002654"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBC2450746.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGE"
BASE COUNT          921 a          369 c          553 g          813 t
ORIGIN      
        1 gtatcaaaac taacatatca gtttgaggag gttaattatg gtttcgatta aaaattggca
       61 aaattctatc gaagaattca atgaaatgca gattctactg ccgtgttttg atgtcaaaga
      121 agttaagcaa gaaggccagc aacatccagt atggcttcat tttggtggag gaaatcttta
      181 tcgaggattt catgcagaaa ttgctcaatg cttagcagat caaaatgaat tagaaacagg
      241 agtagttgtg tgtgaaacat ttgatgaaga agtagtggaa aaagcttatc atgactataa
      301 caatgacatt ttggaaattg ttatgagtgg agatggtgaa ctcaaaaaaa gacttttggc
      361 agtaacggct gatagtttat attgcaatcc gaagaatata gcggactatc aaagagtggt
      421 taaatatttt gaagatccta aattacagtt tacaacgttt actattactg aaaaaggtta
      481 cagtttaaaa gattctaaag ggcaattctt ttcggtagtc aaagctgata tcaaatctgg
      541 acctagcaat cctcaacata cgatgagtat tgtaacatcc ttgttatata gcagatttaa
      601 tgctggagag ttgccgattg cgatggtttc tactgataat ttctcacaga atggtcaacg
      661 ttttcaagat agtattttta cgattgcaga agcttggcaa aaagaaggat atgtttctga
      721 aaaatttatt gcttatttaa aggataaaga aaaagtgact tttccttgga gtatgattga
      781 tcgaattacg ccaaatcctt cagagaatgt aaaggaagat ctattgatga tggggctcga
      841 ggatttagat atcattcaca caaaaaaaca taccaatatt gctgctttca ctaatactga
      901 ggtagttcat tatttaatta ttgaagatag ttttccaaat ggccggccta aattggaaaa
      961 ggctggtgtg attatgacag atagagagac ggttgataaa gcagacatca tgaaggttac
     1021 gacaagcttg aatccacttc atactgcttt ggcagtaaca ggttgtctgc taggatatca
     1081 atcaatttca gcagagatgc aggatgatga tttggttggt ttaatcaaag gaataggtta
     1141 tttagaaggt ttaccagtag ttgaaaatcc aggcattatt gatccaaaac aatttattga
     1201 tgaagtaatt aataaacgtt tgcctaatcc ctatattcca gatacaccac aacgaattgc
     1261 aacagatact tctcaaaaag tagcgattcg ctatggtgaa acaattaaaa agtatgtcga
     1321 aaaagacgga aatgcagaaa atctaacatt tattcctatc gctattgctg cttggttacg
     1381 ttatttactg gcagtcgatg atcaagggga aaattttgtg ccaagtcctg atccgttatt
     1441 ggtagagttg caggataaat tatcagtatt gaaattaggt gaacaagatc cttcaaagat
     1501 tcattgtgcg gtacaggaaa ttttagaaaa taaagaaatc tttggtttag atttatatca
     1561 agttggggta ggaaagaaaa ctgaaaaaat cttttgcgaa atgctagtag gtcctcaagc
     1621 agttcgtaaa acaatcaata aatatataac agaagttgga ggaaagtaac tatggaaatg
     1681 acttttagat ggtatggggc aaatgaagaa aaaatctcac tacaagaaat tcgtcagatc
     1741 ccagcaatga aaggaattgt tggaacatta atggatatac cagccggaga ggtttggccg
     1801 agagaacgaa ttaaatcttt aaaagatgaa gtcgaagcca atggacttac cttgaaagtt
     1861 attgagagtg ttaatgttca tgatgatatc aaaattggtt taccaacacg agatcaatac
     1921 attgagaatt ataaacagac gattcgcaat ttggcagaat ttgggattga ggttatctgt
     1981 tataatttta tgccagtatt tgattgggta aaatcggatt tggattatcg tttagatgat
     2041 gggtcttcta cgttagccta tatttcaaaa aatatcccta ctgatccgga ggaaattatt
     2101 agaaaagtag aaaattctac taataatttt gaattgccgg gttgggaacc tcatcggtta
     2161 tcacaaatta agcaattatt agaagcctat aaagaagttg atgaagagaa attacgagag
     2221 aactttgcat attttctcaa agcaattatc ccgacatgtg aagaagttgg gattaaaatg
     2281 gcagttcacc cagatgatcc accatattcc attttcggat tacctcgtat tgttaaaaat
     2341 cgtgacgatt tagattggtt gtgtaatgta gtggattctc cttctaatgg tattacatta
     2401 tgtactggaa gtattgcaga acgaccagat aatgatgtct atgaaattct atctgaattt
     2461 tgtcaacggg atcggattcc ttttgcacat gttcgtaata tcaaatattt aagtgacaat
     2521 ggactcgatt tttacgaagc gccgcataaa tcagaatatg gttctctaga tatgtataag
     2581 atattaaaaa caatgtacga taatggattt aatggctata ttcgtccaga tcatggtcgg
     2641 atgatttggg gagaaa
//
LOCUS       DADXTG010000204         2650 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.209, whole genome shotgun sequence.
ACCESSION   DADXTG010000204 DADXTG010000000
VERSION     DADXTG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.209"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            36..197
                     /locus_tag="IWI57_002655"
     CDS             36..197
                     /locus_tag="IWI57_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450747.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IWI57_002656"
     CDS             210..713
                     /locus_tag="IWI57_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBC2450748.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IWI57_002657"
     CDS             773..1162
                     /locus_tag="IWI57_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2450749.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IWI57_002658"
     CDS             1149..>2650
                     /locus_tag="IWI57_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBC2450750.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DADXTG010000205         2643 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.210, whole genome shotgun sequence.
ACCESSION   DADXTG010000205 DADXTG010000000
VERSION     DADXTG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.210"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IWI57_002659"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IWI57_002659"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBC2450751.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          951 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaa
//
LOCUS       DADXTG010000206         2614 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.211, whole genome shotgun sequence.
ACCESSION   DADXTG010000206 DADXTG010000000
VERSION     DADXTG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.211"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            192..416
                     /locus_tag="IWI57_002660"
     CDS             192..416
                     /locus_tag="IWI57_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBC2450752.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(413..568)
                     /locus_tag="IWI57_002661"
     CDS             complement(413..568)
                     /locus_tag="IWI57_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450753.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(725..880)
                     /locus_tag="IWI57_002662"
     CDS             complement(725..880)
                     /locus_tag="IWI57_002662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450754.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            952..1182
                     /locus_tag="IWI57_002663"
     CDS             952..1182
                     /locus_tag="IWI57_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBC2450755.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            complement(1179..1337)
                     /locus_tag="IWI57_002664"
     CDS             complement(1179..1337)
                     /locus_tag="IWI57_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450756.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(1350..1616)
                     /locus_tag="IWI57_002665"
     CDS             complement(1350..1616)
                     /locus_tag="IWI57_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450757.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            1816..2520
                     /locus_tag="IWI57_002666"
     CDS             1816..2520
                     /locus_tag="IWI57_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBC2450758.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
BASE COUNT          818 a          475 c          457 g          864 t
ORIGIN      
        1 taaccacttc ttctggcgtt cacgccattg ttcccctagc aatctaggtt tcatttcctc
       61 gtggtattcc tgatttagtt ttcgcatcaa gtgttgatgc tcttgtaatg tcatttgatc
      121 cctcctttcg tttattgaat ttctccattt ctatttgata aaatatttct atagaaatgg
      181 aggtgaaaac tatgggaaaa aatcaacatg ttgtacctaa ttccaatggt ggttggaatg
      241 tcaaaggtgc tggaaattct aaagcgactg cacatactaa tacaaaatca gaagcagtaa
      301 aaatcgctag agagatttct aaaaatcaag gatctgagct atttattcat ggcagagatg
      361 gaaaaattca aagccgtgat agtcacggaa acgatccaca cccaccaaaa ggttaatcat
      421 atttaggagt taatcgaata cgataaccgt cagcaggagt tgcatcggtc aatgtaactt
      481 ctgctacttt ttccccttct tctgtttcaa taattaatct tgtgtaattg ttctcattca
      541 aaatccctat ttttgattct tccttcatgt catccctcct tcgtctctcg ccgtcaccac
      601 ctccaagaag aggagttgcc aaaatgaaga aacgtggact ttggcaaact tgaaaggaga
      661 gcagcaactc ctcttttcgg aagtggcgag tgtgtgatat aatattattg ttctatggtg
      721 cccactactt gcttgccggc tgtgtgggct tttctttttg ttcaattatg gttgccacca
      781 cagtaactcc ccaatgtcta gaacgtattc tagccatttc atctgctaat ctttgaagat
      841 taggtgtgcc attcaaagtg atttttggct tgttactcac aatctcacct ccatcagttt
      901 gttttctttt cacctcttaa tagaattaac ttgaaagcga ggtgaattgt tatgggtaaa
      961 aatcaatggg tatctcctag aaatggcaaa tgggctgtac gtggtgaagg taattccgaa
     1021 gatacaaaac tatttgataa taaatcagat gctgttgagt atggtaaaaa gatttctaaa
     1081 aaacagcaat cagagtttat tgtccaaaaa aagaatggac aaatacaatc aaaagatagt
     1141 tttggaaacg atcctatacc tccacgtgat aaagaacatt aatcatatct aggggtcatt
     1201 cgaactcgaa aaccttcgca tgattcaata tcattcgccg taataactgc gattgttttc
     1261 gggttctgct cgtctgtttc gacaacaatt tttgtatatt cttcaagagt cggtactttt
     1321 atattcttct cttccactat tcacacctcc tagctgactt gttttgtttc acctgttatt
     1381 gatttcggtt tttccgaatc agggtctaaa aaaatatcgg aaactctaac acccaatgcc
     1441 ttagcaattt tttctagagt ggcgtacttt ccgttcctca ttttatctac atcgttttcg
     1501 taaataaata ttgttcttga tgtcactttg cttttccttg acagttcttc ttttgaataa
     1561 ccctttaaag ctcttaattg tctcaatgta tatttttctt tcttcgcggt atccatattt
     1621 tattcgcctc ctctctttaa ctttctgact aaattatata cttcggtttt tccgaagtca
     1681 agtgcatatt tcagttttcc tgaaatcgat ttctattttg cgtaaatact tctttaaaaa
     1741 cactaaaata ttttctaaga aacctgttac attttttccg aaacggtgtt acaattaact
     1801 caaggaggtg ttactatgtt cgctgaaaat ctaaagtttc ttagagaaaa gtataatatt
     1861 gatcaacaag aactagccga aaaactagga agaaaaagtt catcctcaat aagcgaatgg
     1921 gaaaaaggaa aatatacacc aaaattaaaa accttaaatg agatagctaa gatattcaat
     1981 gttgatatag atgatttgat gaatattgat ttatctatat ctaatcagat atataatatc
     2041 cttcctatat ataatcagct taacgacgaa aataaagttg ctacctatga atttgcaagt
     2101 aatcgattag aagaacaaaa acaagaaaat gtcctagatt ttcctaaaaa agaaaaaatc
     2161 ccaactgttc acaattcggc ttcggctgcc aatccaacag aacttactta cggtgatacg
     2221 gttgtcgaag aggaagaatt tgagagagtt ccaagtgctg ctgacttcgc agtacctatt
     2281 ataggcgatt caatggaacc tgttatcagg aatggacaat tcgtattcgt taaagaacaa
     2341 ccggacgtag aagatgggga aattgctatt gtggaattag gtggtgacgg agttacatgt
     2401 aaagagatat ataaagatta cgaaaatcaa acaatcattt tacgttctat aaacgactta
     2461 tacgaagata gaattgtatc gccagaacag attagaatta tcggaaaagt tgtattttaa
     2521 aaaaattccc atgccggagc cgtcaacttc ggtaagggtt tatatgattt tgacaattat
     2581 tttataaaag aagcgaggcg ttttgaatgg gagt
//
LOCUS       DADXTG010000207         2608 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.212, whole genome shotgun sequence.
ACCESSION   DADXTG010000207 DADXTG010000000
VERSION     DADXTG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.212"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            122..1753
                     /locus_tag="IWI57_002667"
     CDS             122..1753
                     /locus_tag="IWI57_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBC2450759.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1756..>2608
                     /gene="uxuA"
                     /locus_tag="IWI57_002668"
     CDS             1756..>2608
                     /gene="uxuA"
                     /locus_tag="IWI57_002668"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBC2450760.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNG"
BASE COUNT          911 a          365 c          538 g          794 t
ORIGIN      
        1 acaataattc tgacatggta gaaaaaagaa tatttttagt tttttcttat tgacagcgat
       61 tacattcgat tttatactac aattgtaacg gaaactaaca tatcagtatg gaggggtaaa
      121 tatggttttg attaaagact ggcaagattc tatcgaagga ttgaacaaaa tgcaagttca
      181 actaccgtat tttaatgtcg aagcggtcaa acaagaaggt cagaaacatc cagtgtggct
      241 tcattttggt ggaggaaatc tttatcgagg atttcatgca gaaattgctc aatgcttagc
      301 agatcaaaat gaattagaat ccggagtagt tgtgtgtgag acatttgatg aagaagtagt
      361 agaaaaagct tatcatgact acaacaatga tattttggag attgttatgg gtgaagatgg
      421 tgaactcaaa aaaagacttt tagcagtaac agctgatagt ttatattgca atccaaagaa
      481 tataacggac tatcaaagag tggttaaata ttttgaagat cctacattac agtttacaac
      541 gtttactatt actgaaaaag gatacagttt aaaagattct aaaggacaat ttttttcggt
      601 agtggaagca gatataaaat ctggacctac caatcctcaa catacgatga gtattgtgac
      661 atccttgttg tatagcaggt ttaatgctgg agagttaccg attgcgatgg tttctactga
      721 taatttctca cagaatggtc aacgttttca agatagcatt tttatgattg cagaagcttg
      781 gcaaaaaaaa ggatacgttt ctgaaaaatt tattgcttat ttaaaggata aagaaaaagt
      841 gacttttcct tggagtatga ttgatagaat tacgccaaat ccttcggaaa gtgtcaaaat
      901 ggatttattg aaggcgggtt tcgaagactt agatataatt catactgcga aaaataccaa
      961 tatcgctgct tttaccaata ctgaaaaagt ccattatcta gtcattgaag atagttttcc
     1021 aaatggtcga cctaaactgg aagcggctgg tgtgattatg acagatagag aaacagttga
     1081 taaagcagat atcatgaagg ttacgacatg tttaaatccg ctccatacgg cattagcaat
     1141 aacaggttgt ttgttggggt ataaatcaat tcaggcagaa atgcaagatg atgatttggt
     1201 tggcttaatt aaagggatag gttatgtaga aggcttacca gtagttgaaa gtccaggcgt
     1261 tattgatcca aaacaattta ttgatgaagt aatcaataaa cgtttgccta atccctatat
     1321 tccagataca ccgcaacgaa ttgcaacaga tacttctcaa aaagtagcga ttcgctatgg
     1381 tgaaacaatt aaaaagtatg tcgaaaaaga cggaaatgca gaaaatctaa catttattcc
     1441 tatcgctatt gctgcttggt tacgttattt attggcagtc gatgatcaag gaaaaaattt
     1501 tgtgccaagt cctgatcctt tattggtaga gttgcaggaa aaattctcag tattgaaatt
     1561 aggtgaacaa gatcctgcaa agattcattg tgcggtacag gaaatcttag aaaataagga
     1621 aatatttggt ttagatttat atcaagttga gataggaaag aaaattgaaa aaatcttttg
     1681 cgaaatgcta gtaggtcctc aagcagttcg taaaacaatc aataaatata taacagaagt
     1741 tggaggaaag taactatgga aatgactttt agatggtatg gggcaaatga agaaaaaatc
     1801 tcactacaag aaattcgtca gatcccagca atgaaaggaa ttgttggaac attaatggat
     1861 ataccagccg gagaggtctg gccgagagaa cgaattaaag ctttaaaaga tgaagtcgaa
     1921 gccaatggcc ttaccttgaa agttattgag agtgttaatg ttcatgatga tatcaaaatt
     1981 ggtttgccaa cacgagatca atacatcgag aattataaac agacgattcg caatttggca
     2041 gaatttggga ttgaggttat ctgttataat tttatgccag tctttgattg ggtaaaatca
     2101 gatttggatt atcatttaga tgatgggtct tctacgttag cctatatttc aaaaaatatt
     2161 cctactgatc cagaggaaat tattagaaaa gtagaaaatt ctactaataa ttttgaattg
     2221 ccgggttggg aacctcatcg gttatcacaa atcaagcaat tattagaagc ctataaagaa
     2281 gttgatgaag cgaaattgcg agagaacttt gcatattttc tcaaagcaat tatcccgaca
     2341 tgtgaagaag ttgggattaa aatggcagtt catccagatg atccaccata ttctattttc
     2401 ggattacctc gtattgttaa aaatcgtgac gatttagaat ggttgtgtaa tgtagtggat
     2461 tctccttcta atggtattac gttatgtact ggaagtattg cagaacgacc agataatgat
     2521 gtctatgaaa ttctatctga attttgtcaa cgggatcgga ttccttttgc acatgttcgt
     2581 aatatcaaat atttaagtga taatggac
//
LOCUS       DADXTG010000208         2554 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.213, whole genome shotgun sequence.
ACCESSION   DADXTG010000208 DADXTG010000000
VERSION     DADXTG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.213"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..1141
                     /locus_tag="IWI57_002669"
     CDS             14..1141
                     /locus_tag="IWI57_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2450761.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="IWI57_002670"
     CDS             complement(1359..2501)
                     /locus_tag="IWI57_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2450762.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYMLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTIEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          853 a          463 c          418 g          820 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctgtgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaatgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaaca tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat agga
//
LOCUS       DADXTG010000209         2518 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.214, whole genome shotgun sequence.
ACCESSION   DADXTG010000209 DADXTG010000000
VERSION     DADXTG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.214"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..769)
                     /locus_tag="IWI57_002671"
     CDS             complement(<1..769)
                     /locus_tag="IWI57_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HBC2450763.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
     gene            complement(974..1324)
                     /locus_tag="IWI57_002672"
     CDS             complement(974..1324)
                     /locus_tag="IWI57_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450764.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1919..2365
                     /locus_tag="IWI57_002673"
     CDS             1919..2365
                     /locus_tag="IWI57_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBC2450765.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
BASE COUNT          764 a          403 c          510 g          841 t
ORIGIN      
        1 ctaaagcatt gtaaaaaaat ggacgtctaa ctatatattc gcttgtatat agtatatctt
       61 gattcgtgga atcatagggt ttctttacca tctttttggt gacagttggt agtttgagat
      121 tccgggactt gaaaaaagct tttttcttat gctctttcaa ttccagctct ttatcgaaat
      181 atttgctgat atagcgcccg cgattttcga tactgtctac atcgatcttg ttgatcttaa
      241 cgaaaccctg accccaaatc gtagttaacc ggttaaatga gataaagggg aaatcaaaca
      301 agataatatg gtagtggatt gcaccgcgtt gctgcttttc ccacgtggcg agatacttta
      361 aatttgcttt tttagtttta tagaggtcat aattcagtcg tttgataaat tttttaaatt
      421 cattatttgc atactcaatg tcttgaatgt tttctttgaa agtgagagtt aaaaatttcg
      481 tttgattgtc aaaattcgtg tctacaaggc gggcaatctc aaatcgcata ctttgataat
      541 gtttctgttt gcgttttaaa ctgtcatatt gtccttgagc ggataattca tcatagtttt
      601 ttcttttttc aagagtaata tcattttcat aaatccaagc taatttttgt tttcgttcca
      661 gatttacttc gttttcgatc gttttacgtt tgggagtaat gattgcttca taatcataaa
      721 tttcaatata cgtgggagtt tcaatgattt tctgattata agcttgcaaa aatcatcact
      781 ccttgttgcg tattcgattg tcaaagaacc agtcattttc tcctgatgtt gatctataaa
      841 tcaagttaag ggggaaggcc tgcgtagcag cccttctagc ctatggggtg ttgggcggtc
      901 ttaacggaca gaaacggctc tgtgggcgct gccccgccgt ttcgtcctaa ccacccaaca
      961 ctccatagta tagttagttt tgtgtagtgg taccttgttt tctgggaatc aaagttgccg
     1021 tatcagcggt tgccgataac ccataactga caaattgtcc attcgagcgt ccccaaggta
     1081 caatcaccaa attggtaaat gtaattatgg ctaaaccttg ttgggtaagt tcttccggcg
     1141 tgatcacagg atgttcagat ttgactttga cactgatttt ttcgtagcga agttttggta
     1201 ggatacattc gtagctccaa cctagaattt cgccctcctt ttgccagcga gtgacattga
     1261 cgacttggta ttctttttcg agtgtttctt ttggtaaact gagatcggta atttttattg
     1321 gcatcacaat tctccttttt tatacgaaag tttatacgat ttgattaaaa aatatagatg
     1381 gccatctacg acttgtatta tatacgagaa cttatacgta gtcaagagat tttctaaaaa
     1441 aatatatacg attttttata cgagtgttat aatgaaaaaa atgataagga ggaatttgaa
     1501 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
     1561 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
     1621 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
     1681 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
     1741 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
     1801 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
     1861 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
     1921 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
     1981 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
     2041 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
     2101 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
     2161 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
     2221 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
     2281 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
     2341 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
     2401 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
     2461 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcac
//
LOCUS       DADXTG010000210         2514 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.215, whole genome shotgun sequence.
ACCESSION   DADXTG010000210 DADXTG010000000
VERSION     DADXTG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.215"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            10..837
                     /locus_tag="IWI57_002674"
     CDS             10..837
                     /locus_tag="IWI57_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBC2450766.1"
                     /translation="MWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAALG
                     FDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSVI
                     FGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFIFG
                     IEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLE
                     AKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
     gene            937..1371
                     /locus_tag="IWI57_002675"
     CDS             937..1371
                     /locus_tag="IWI57_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBC2450767.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            1551..1961
                     /locus_tag="IWI57_002676"
     CDS             1551..1961
                     /locus_tag="IWI57_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBC2450768.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            2018..2458
                     /locus_tag="IWI57_002677"
     CDS             2018..2458
                     /locus_tag="IWI57_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2450769.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
BASE COUNT          784 a          416 c          497 g          817 t
ORIGIN      
        1 agacaattta tatggacata cattaagatt atttttgccc tcttcatatt aggagtcgct
       61 atcaatatgt ttttagggcc tcatcatatt gctgctggag gtgtcagcgg tctaggaata
      121 ttactggaag ctgctttagg ctttgaccga gctatagtta ttttagtgtt gaatatcgtt
      181 atgttggtac tagctcttct atttttaggt aaaaaaccct tttttaaagt acttttcgga
      241 agcttggttt ttcctttaat cattgcaata gttccagaaa caatgattac ttcagatcgg
      301 ttgttgtctg tcatttttgg aagtgcgatt tttgctttag gagttgcgat tttatataaa
      361 aacaactctt ctagcggcgg tacgacgatc cctcccctca tttttaaaaa atatttccac
      421 ttgaatactt cgattggctt attttttaca gacgcattcg ttgtttctct taatctgttt
      481 atttttggaa tagaagagtt tctttatgct attttatcta ttgcaatcac ttcaattgtc
      541 atgacttata tcgaaacagg tatcaatcga aagaaatctg ttatggtcat gagtgaacat
      601 tatttggaag acatccgcct tcgcttaagt gctgagattg gacgtggcct taccctgcta
      661 gaggcaaaag gcgggtataa tcgaaagtca aaagaggttt tattgatcgt tgtgactgat
      721 catgaatttt cccgaatgaa accattgata gaagaaatcg atccaagtgc ctttgtcatt
      781 gtcagcagcg tagcagaagt aatgggacgc ggatttactt atcatccaat cgaatgagtt
      841 ggtaagtaaa tccgctattt ttttgaatgc gttttcttta gctggtataa taaaagaatc
      901 tagtaactgt tcgactgaaa aggagaggag atctttatgc ataaaggaac ggaaatcgat
      961 tttgtattaa atgatagttt gaaagcacta gaactgtatc aaacaatctt tgatgaaata
     1021 caactgattg aggcaacaga ttaccctaga ggcaataatg aagtcatttt taccttgtat
     1081 ggtacgagat tccatatgct ggatgaaaac acgtctcttg gtttgactgc tcctactgct
     1141 gaacatccaa atacgatctg gttcaatatc atcgtctctg atatcgagga cacacataat
     1201 aaagcattac gaaatggctg tgtagagatt caagaaatga cagaaatgat ggactttggt
     1261 gtgactaact caatgttcag agatcctttt ggttatttat ggatgcttca tcaggtccat
     1321 cgtgaagtaa tttttgaaga aagaaaacaa ctttttgaga aatatatgta atgtttatga
     1381 tgagattatt ttttgtctct gatatttttt gtatatgttt gctattcgag aaagcgcgtc
     1441 tgtttcatgt gctttctctt atttatctta tgccaatcca tttctttgct ttccacaatt
     1501 atattgtgta aaattcaatt gtataaaata taattaaagg tggtaatttt atgaaaaaaa
     1561 tgtattctac gaaaatgatc aacacaggcg gacgctctgg agaagtgcat aatcccgatc
     1621 gtacattcga attgaatatt gcagctcctg gtaaacgcgt cgaaaatgca accaaccctg
     1681 aacaattgtt tgcagctggt ttcagtgcat gttttaacag tgcattagaa ttgatcaaac
     1741 agcaaaaaaa tatctctggg gcttctactg tcagtgcaca agtttccctt tattcagaaa
     1801 gtgaaacgag ctttgtctta ggtgtagaga ttgaaggatc tatcgaaggt ctttctctgg
     1861 aagaaacgca agaattatta gaggcagcgc ataaagtgtg tccgtattca aaagctacag
     1921 ctggaaatat agaagttact ctgaaagcag ttagtgaata gacttttttg tgtaaatccg
     1981 ctatgataca ctatagtaaa acagcggagg tggaaaaatg gaagatctat atttagttaa
     2041 tcaagtatgt tttcctttat atgccgcctc taaagaaatc attagacact acactcccct
     2101 cctcaagccg ctgaatctta cctataccca gtatcttgtg atgttagttt tatgggaaaa
     2161 gaaaaagact tcagtgaaag agctaggaaa tgatttgtgt ctggattctg gtacgctgac
     2221 tcccttacta aaaaagttag cagaaaaaaa ttatattaat cgtcagcgtg aaacgggtga
     2281 tgaaagaatc gtaaatgtta cgctgactgc tcaaggtgct gcgttgcaaa aacaagcaga
     2341 aggtattcca caaaagatca agtcttatct tccacttgaa caggaagaat tggatacact
     2401 gcaacatctg acccgaaaaa tgctagataa ttttcatcaa gaccagaaaa agggctgaaa
     2461 ctgctagatg aacgatttta tgaggaaaac caactcataa gataacgtca ttta
//
LOCUS       DADXTG010000211         2405 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.216, whole genome shotgun sequence.
ACCESSION   DADXTG010000211 DADXTG010000000
VERSION     DADXTG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.216"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..310
                     /locus_tag="IWI57_002678"
     CDS             <1..310
                     /locus_tag="IWI57_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450770.1"
                     /translation="IVLGIVVLLITVKIVDYFANTPEEQIQRKLPEFERVPPYDPRDN
                     SFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIVKAKVSKRFIFNPKLEIYSVVIE
                     "
     gene            635..1252
                     /locus_tag="IWI57_002679"
     CDS             635..1252
                     /locus_tag="IWI57_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2450771.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1293..1592
                     /locus_tag="IWI57_002680"
     CDS             1293..1592
                     /locus_tag="IWI57_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBC2450772.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1626..1793
                     /locus_tag="IWI57_002681"
     CDS             1626..1793
                     /locus_tag="IWI57_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBC2450773.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1838..2401)
                     /locus_tag="IWI57_002682"
     CDS             complement(1838..2401)
                     /locus_tag="IWI57_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2450774.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          830 a          372 c          457 g          746 t
ORIGIN      
        1 tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt actttgcgaa
       61 tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac caccttatga
      121 tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg aaatgtggtt
      181 tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca attatattgt
      241 aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat atagtgttgt
      301 aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc tgatgtgaaa
      361 aataaaatat accatggaag acataaaaga aaaactggat aactagttta tttaatggca
      421 gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc atgcgggagt
      481 gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct ttgtttatgt
      541 atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg attgttttga
      601 aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt atatccgggt
      661 cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat taggaatccc
      721 tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc ctgaatatca
      781 attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat tagatagact
      841 aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg aaaagcagat
      901 tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc aactagatgg
      961 gttagagacg cttatctctg acttaattct gcaattactc agctatatgg ccgaagacga
     1021 gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac aaaagggtgt
     1081 aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct attctgactg
     1141 gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga aacgaaatac
     1201 cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt gatagatata
     1261 acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa agggtcgttt
     1321 ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt cactctttaa
     1381 acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt ggtcagtatt
     1441 ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg cgggacttga
     1501 ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg ttaattagtc
     1561 aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca aggaggaaaa
     1621 tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt tttaatcgaa
     1681 atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt tatcaaaaaa
     1741 attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca tagaaacgaa
     1801 atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc gctttcttta
     1861 ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca actggaatgc
     1921 cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga tctgttgagt
     1981 taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc aataatactc
     2041 cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa cttagcagtg
     2101 ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta aaatctaaaa
     2161 actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca actgtatttt
     2221 ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg caatcacctt
     2281 cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta ccactggctt
     2341 tttcttggaa taatttatca atgttaagtc cttcggttct gttgcgaagt ttgaaaatca
     2401 aacgc
//
LOCUS       DADXTG010000212         2404 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.217, whole genome shotgun sequence.
ACCESSION   DADXTG010000212 DADXTG010000000
VERSION     DADXTG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.217"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            44..1185
                     /locus_tag="IWI57_002683"
                     /pseudo
     CDS             44..1185
                     /locus_tag="IWI57_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1311..2390)
                     /locus_tag="IWI57_002684"
     CDS             complement(1311..2390)
                     /locus_tag="IWI57_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBC2450775.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
BASE COUNT          971 a          452 c          418 g          563 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
      481 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
      541 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
      601 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
      661 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
      721 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      781 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
      841 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
      901 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
      961 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1021 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
     1081 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1141 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     1201 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
     1261 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
     1321 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
     1381 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
     1441 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
     1501 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
     1561 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
     1621 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
     1681 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
     1741 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
     1801 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
     1861 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
     1921 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
     1981 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
     2041 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
     2101 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     2161 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     2221 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     2281 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     2341 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     2401 aata
//
LOCUS       DADXTG010000213         2401 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.218, whole genome shotgun sequence.
ACCESSION   DADXTG010000213 DADXTG010000000
VERSION     DADXTG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.218"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWI57_002685"
     CDS             207..2069
                     /locus_tag="IWI57_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2450776.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="IWI57_002686"
     CDS             2069..2401
                     /locus_tag="IWI57_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBC2450777.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 a
//
LOCUS       DADXTG010000214         2364 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.219, whole genome shotgun sequence.
ACCESSION   DADXTG010000214 DADXTG010000000
VERSION     DADXTG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.219"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..780)
                     /locus_tag="IWI57_002687"
     CDS             complement(<1..780)
                     /locus_tag="IWI57_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2450778.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSM"
     gene            complement(909..1556)
                     /locus_tag="IWI57_002688"
     CDS             complement(909..1556)
                     /locus_tag="IWI57_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBC2450779.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(1640..>2364)
                     /locus_tag="IWI57_002689"
     CDS             complement(1640..>2364)
                     /locus_tag="IWI57_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBC2450780.1"
                     /translation="LADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQFVTG
                     IQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVALFD
                     TAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQQL
                     TTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQI
                     VENICYRNAYNYFNFFEIESRK"
BASE COUNT          759 a          493 c          304 g          808 t
ORIGIN      
        1 catactcatt gttaaaagct gagcaagcaa caaaaaaata gctgcagcta ttatagaaag
       61 caacgctcta tttttagcta atccttttaa agatttcaac aatgaaactt ttaactgcga
      121 ttcatcactt ttaacccgtt ctacagttaa tttataacat attacgtaac aaataactgc
      181 acacactgaa aatactcctg ccacaattgt aaaagttgat ccaccacgta caatttgagc
      241 tccagaacta tctcttgtat aaataaaaat tggtgttaac acgccaatga acatacctgc
      301 taaggtagct cctatactac gaaatgttga taacgctgtc ctttcttctg caacagaagt
      361 gatagctgat gccattgaac cgtaaggaat attaatagat gtataaaaaa tacttcccca
      421 tagtatatat gttacataca tatatataac tttaatactc atagaagctg atgccattga
      481 tgtttgatac attaaaaaac ttgctaaagc gactggacca ctcatccgta aaatccacgg
      541 tttaaatttg ccagttttag atggttttga tccatccaca atacgcccca ttgttatgtc
      601 tgtaactgca tcaataaatc ttgctactaa aaataatgtt cctacggtag ctccgcttat
      661 ccccaatact tttgtataaa aaatcattaa aaaagaacta gcaaaaatga aagtgaaatc
      721 attcccaaaa tctccaaaca tatatcctat tttgtctctt agaccaaatt ttctttccat
      781 ataatttgcg ctccttccta ttgttaagcg ttttcattct gttgaactgc caacttacgt
      841 actatactct gaagtataaa gagttagtat ttatttttta cattttatag tattatttat
      901 tttttctcct aataattctt tatctcataa aacttatcta tatatttact agctattcga
      961 gttacttctt caaaatcacc tttttcaaca ggagccaaca aattcccacc tacaccaact
     1021 gcaacaacgc ctgcttcaaa ccactcttct aagttatcta gacttactcc gccagtaggc
     1081 attatattta catatggtaa tggagcttga tacgttttta caacagatgg accaaaagca
     1141 gaacctggaa atagtttaat gatttctact ccagcggtaa gtgcctcctt tatctcagta
     1201 attgtcatac aacctggtaa ataaggaatt tgatataaat tacaaatctc tgctgtttgt
     1261 cgatcaaaag atggacttac gatatattct gctccagcca taattgcaat acgagcagta
     1321 aacgcatcta aaactgtacc ggctccaatt attacatctt tttgatcagc atatttttta
     1381 gcaatttctt tgattatttc ttcagcattc ggaactgtaa aagttagttc aatcccttta
     1441 attccacccg cagtaattgc atttattgct ttaatggcct catctttatt ttttccacgt
     1501 acaaccgcaa tgactccagc cgcttctaac ttacttaaaa tttctacttt tttcatatct
     1561 atctccttct tttatggata catactctag ctagttgtac taacttacaa taaataaatt
     1621 taagctttta ttttttgtat tattttctac tttcgatttc aaaaaaatta aaataattat
     1681 acgcattacg ataacaaata ttctccacta tctgactaag atacatttca tcatctggaa
     1741 ttcgacctgc tttaacccac tcgcttagta attgacataa tactcgacgg aaatactcat
     1801 ggcgaggata gcttagaaaa cttcgagaat cagtcaacat acctgtaaaa ttcgacaaca
     1861 aactttgttg cgccatcgta gtcaactgtt gacgcattcc ttcagctgta tcattgaacc
     1921 accaagcaca tcccaattgg attttttgaa tcataccccc ttggaagctt tgcattgctg
     1981 ttgctagtgg aagccaatcg gttggattta atgaatataa aattgttttt ggtaagcacc
     2041 cactcttttc agcagtgtcg aacaatgcta caacattttg tgtcaaacta gcttgatccc
     2101 ccatcgaatc accaccgaca tctactccaa tttgttcctt taatatactt gaattatttc
     2161 ttaaagcatt taaatgcatt tgcatcgtcc aaccatatct tgtatactca gacatcaaat
     2221 gtaattgaat acctgtaaca aattgtgcaa tttctgataa atggaaatcc gtttttcctg
     2281 ctaaagcttt tattaacaga ttgtcgagta ttttttcatc tgctatttca taaaaataag
     2341 tattcaaacc gtgatctgct aatc
//
LOCUS       DADXTG010000215         2361 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.220, whole genome shotgun sequence.
ACCESSION   DADXTG010000215 DADXTG010000000
VERSION     DADXTG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.220"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..217
                     /locus_tag="IWI57_002690"
     CDS             <1..217
                     /locus_tag="IWI57_002690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBC2450781.1"
                     /translation="KSRAEEILDLTNQTTQEFENRKKQLFSGRFTIGCVEADNSNTLA
                     LLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            230..691
                     /locus_tag="IWI57_002691"
     CDS             230..691
                     /locus_tag="IWI57_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBC2450782.1"
                     /translation="MLDLAILLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVIT
                     KEDFANVPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVI
                     EGAVSHLSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            953..1768
                     /locus_tag="IWI57_002692"
     CDS             953..1768
                     /locus_tag="IWI57_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBC2450783.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            1778..>2361
                     /locus_tag="IWI57_002693"
     CDS             1778..>2361
                     /locus_tag="IWI57_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBC2450784.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQW"
BASE COUNT          709 a          358 c          478 g          816 t
ORIGIN      
        1 aaaaagccga gcagaagaaa ttttagattt aacgaatcaa accactcaag aattcgagaa
       61 cagaaaaaaa caattattca gcggccgatt tacgatcggc tgtgtggaag ctgataattc
      121 gaatacattg gcactattac tagaaaagtt gatccgtgat tatcccagcg ttcaatttca
      181 tatccacagc ggaacaagtg atgatatcac ggagtgacta gaaaaaagcc tgttagattt
      241 agcgattttg ctagatccag tagatacaga gaaatacgaa acgattttgt taccaagaaa
      301 ggaaaaatgg ggattgctca ttttagaaaa ttctgtttta gcaaaaaaag acgtgataac
      361 aaaagaagac tttgcgaatg tacctctttt gatctcaaag cgtccagaaa tacagcgaat
      421 gtttgctgac tggttaggca taaaaaagga gcagttgctg atcacaggaa cgtacaattt
      481 gattttcaat gtatttgcgc taattgagaa tcaggtaggt gctgctttag tcatagaagg
      541 tgctgtaagt catctttcaa agcgagtgac attcgttccg cttgacccaa tgattgaaac
      601 gaactgcatg ctagtctgga aaaagaatcg gattttatca cctgtagtca atgaattgat
      661 tcaccgtttc aaagaagctt ttgaacagta ataagtatct ctaatcgaag aaatttcaat
      721 acaaaagcga gacgtatgac agtcttgctt ttgtattttt tcatttttaa atcaaaaaaa
      781 ataaagagat ttacataact tgttaaataa gcgcttacaa acataagaag aacggaaatg
      841 aatcagctat aataaccgtt gttctggatt gtaatcatta caaagaaatg aagattataa
      901 ctttggaggt gttcgaaatg aaagaaaaaa agcaaaaatt cgttcaaaaa acatgccctt
      961 tattcaatgg ttgggtatga ttctgctgat tgtattagct ttattgacga tcattccttt
     1021 tttatggatg gtttctgctt cattcaaagc aaataatgaa gtgttcacga ttccgattca
     1081 atggatccct aaaacttggc atttagagaa ttatctggtt atttggtctc gtattccttt
     1141 agtgacattt tttaagaata cagctttttt aagcgttgtg atcacgttga ttcagttgtt
     1201 tacttctagt tttgctgctt acgggttttc taaactaaat tttaaagggc gcgatatttt
     1261 atttttagca tatatcggca cgattgctgt cccttggcag tcttatatga tcccgcagtt
     1321 cattatgatg agaaaagtgg gtttaacata tacgttgtgg tcgttggtgc tattacaagc
     1381 gttcaatgct tttggtgtgt tcttgttgaa gcaatactac agtagtatcc cagattcttt
     1441 atgtgagagt gcgcggatcg atggcttatc agagtgggga atctatcgca agatcatttt
     1501 acctcttaca aaaccggcga ttgccagttt gacgatcatc acattcgtta atacatggaa
     1561 cgattatatg gggccattca tttatttgtc ttctacggaa aataaaacaa tccagttagg
     1621 gttgaaaatg tttattgggc tatttgatgc agagtacgca ttaatcatgg ctgcctcagt
     1681 tgtttctgtt cttcctgttg cttttatatt tttagcgatg caaaaatatt ttgtagaagg
     1741 gatcgcttca tcaggaatga aggaatagga ggtgattatg aaagagcagc gttttgacaa
     1801 aaatgtttat atggaattct tttattggtt gtatcaatca ttggcaagta gcctttgttt
     1861 ttggtgtgtc aatatcttgt atatagcagc tgcgttcctt ttgtcgatag atggaaggaa
     1921 tgtcttttcc tttatttttt ctttactttt tattggtcca ggttgcatcg tgatgatagg
     1981 tatgacagaa gaattagcaa aagaaaaaag tttttcacca gtacgtttgt tcataaagac
     2041 gtttcgccaa ttttggctaa agggattttt ttattggcta tttgcttgga tagttagtgt
     2101 gataatgatt tttgattgtt tcttttttat acgtttttct tatggaaaat ggttgattcc
     2161 gttgtttgtt ttacttgctt gtttatcagt ttcctttagc atcaactact ggtattttca
     2221 ggtgagaaat ccagcgtcga aaccaaatca agttctacgg atagcgttct actatacgtt
     2281 gaaaaaatgg tatgtgagtt tactagattt tttactattg acaagtttgt ttctcttctt
     2341 tttcgtaaag ccccaatggt g
//
LOCUS       DADXTG010000216         2335 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.221, whole genome shotgun sequence.
ACCESSION   DADXTG010000216 DADXTG010000000
VERSION     DADXTG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.221"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(865..1155)
                     /locus_tag="IWI57_002694"
     CDS             complement(865..1155)
                     /locus_tag="IWI57_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450785.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1363..2055)
                     /locus_tag="IWI57_002695"
     CDS             complement(1363..2055)
                     /locus_tag="IWI57_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBC2450786.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          673 a          434 c          335 g          893 t
ORIGIN      
        1 agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata gtcggcatat
       61 acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc agttctttgt
      121 tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata tttcctaata
      181 gagtataaca tagctttaag cattacttaa agataactta aaagttatct tttaaatact
      241 taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt
      301 cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt
      361 cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata
      421 tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc
      481 gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt
      541 tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt
      601 aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt
      661 cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga
      721 ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat
      781 gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac
      841 atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct
      901 tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca catgatagga
      961 tctcacaaca aattgctttc tattattata ataacctgta acgactaact tcatcccttc
     1021 gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa
     1081 actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc
     1141 atattttttc tccataacct tcacctcaat accattatac gaacatacat tccctttgta
     1201 aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat
     1261 tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagatta
     1321 ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac gcaaatgttc
     1381 ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg ctttttctaa
     1441 cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta atttcttttg
     1501 tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg cgatttgttg
     1561 attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt cttgtagtct
     1621 tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga tctcatctag
     1681 tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga ctaattgact
     1741 ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt catttacttt
     1801 tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt tttgtgctag
     1861 tcgagcggta agatctgtaa gcgcttgctc tttatctgtc aatgtatgat caagctgtgt
     1921 cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa tttcatttgc
     1981 gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc caccagtaat
     2041 aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct aaaagtaagt
     2101 gatctcaaac acaatgtcaa gcgtttttaa attacatgtg taattttaat ataaatacta
     2161 tcaaatactc atttaatcct ccttttttat ctatttaaaa aatctttttt atagagaaaa
     2221 atgcaggtta atactttctt aaataaatat ttatggtgaa caaaaacaaa aaagcaagtt
     2281 ttattaaacg taaccctttt catttcatag attactgtat ttattacaaa aaacg
//
LOCUS       DADXTG010000217         2146 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.223, whole genome shotgun sequence.
ACCESSION   DADXTG010000217 DADXTG010000000
VERSION     DADXTG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.223"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..200
                     /locus_tag="IWI57_002696"
     CDS             <1..200
                     /locus_tag="IWI57_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBC2450787.1"
                     /translation="INANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITK
                     TLNQILNDGEDIKWIQKQIAY"
     gene            173..850
                     /locus_tag="IWI57_002697"
     CDS             173..850
                     /locus_tag="IWI57_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBC2450788.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            840..1982
                     /locus_tag="IWI57_002698"
     CDS             840..1982
                     /locus_tag="IWI57_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBC2450789.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1995..>2146
                     /locus_tag="IWI57_002699"
     CDS             1995..>2146
                     /locus_tag="IWI57_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2450790.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTY"
BASE COUNT          786 a          278 c          440 g          642 t
ORIGIN      
        1 ttataaatgc aaatctaaat gaaaatgaaa ttgaaactgc tttgaataaa atacaagatc
       61 ctcagtttat aaaaagcttg aaaaatgtta agaacatcta cggtgacggc aacagttcta
      121 gaagaataac aaaaacattg aatcaaatat tgaatgatgg ggaagatatt aagtggattc
      181 aaaaacaaat agcttattaa aatatactag tgatccatac attattattg gggctggcgg
      241 acacgcaaaa gtcattattg aaaccttaag attagaaaat aagttaatat atggattttt
      301 tgatgataag caaaataaaa tgcaagatct gaaatatctt gggaaaataa acgaattgaa
      361 tagctgggcg gatagcaaaa aaaaaatcta taaatttatt gtagctattg gtgactgtgt
      421 agtaagaaaa aagattataa aaaaattgaa tatgccaagt gagcaatatg gaatagctgt
      481 tcatccaagt gctatagtgg caaaagaaat ttcaattggt tatggaactg ttatttttgc
      541 gaatgctgta attaacccag atgctgtaat tggagagcat gctataataa acacaggtag
      601 cattgttgag catgattgta gaattaataa ctatgttcat ttatctccag gtgtatgctt
      661 agcgggtgga gtacatgtag gtgtaggaac tcaaatagga attggttctc aatgtatcca
      721 gatgaagaaa attggatctt ggtgtatgat aggcgcaggg agcactattg ttaaggatat
      781 tccctcacat tccttagctt atggcaatcc agctaaaatt aagaaagaag gaattaattt
      841 tgaattctag aattttatta tcaagtcctc atatgggagg aacagaagaa aaatatgttg
      901 ctaaggcttt taaagataat tggatagcgc cattaggtga aaatgtggat aaatttgaag
      961 aaagtattac aaaatatact ggttctaagg gcggagcttc tgcgttaagt tcaggtacag
     1021 ctgccataca tcttgcctta agactgctta atgttggaaa aaacgatatt gtctttgctc
     1081 cttcatttac ttttatagct acagtaaatc ctattttata tcaaggggct gtaccagtgt
     1141 tcattgactc agaacctaat acatggaata tgagccctgt tgcactagag agcgcattag
     1201 aagaagctga aagaaacaac caattaccta aggcaatcat cgtagtgaat ttatatggtc
     1261 aaatggctga ttatgaacga attttagcta ttgcaaacca atttaatgtt ccagtgatag
     1321 aagatgcagc ggaatcgtta ggttctactt ataagggaat ttcgtcaggc ttatttggga
     1381 caattgggat ctattcattt aacggtaata agatcatcac aacctcaggt ggaggagctc
     1441 tagtgtctag tgataaaaaa tatgttgagc attcaagatt tttagcaaca caagcaagag
     1501 atcaagcacc gtattatcaa catagcgaaa taggttataa ttaccgtcta agcaatattt
     1561 ctgccggtat cggacgtgga caaatggaag tattgaatga tagagtagct aaacgaagag
     1621 aaattttcaa tcgatataag gaagcttttt ctggaattga tagaattgaa atgatgccag
     1681 aagtggatgg catggtgcca aatcgttggt tgtctacttt aacgatcaat gattataatt
     1741 ctattgatca agtagttaac gtaataaata aactaaatga aaaaaacatt gaaacaagag
     1801 ctttgtggaa accactacat ttgcaaccag tatttcaagg ggtaaaaatg tattaccatg
     1861 ataataatct tagtgtgtca gaaaagttat ttaatagcgg cttatgctta ccttctggaa
     1921 gtaatatgac agatagtgaa caagaatttg ttattgatga gtttaaaaaa atattcaagt
     1981 aggtgtgata ttttatgaac ataggaatta ttggtttagg ttccatgggg aaaagaagac
     2041 ttagactcat tcaagaacac tttaatgata ttttactatg tggtgttgat actaatgagg
     2101 cgagacgaaa ggaagttgaa aaggaatttg gaataccaac ttataa
//
LOCUS       DADXTG010000218         2118 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.224, whole genome shotgun sequence.
ACCESSION   DADXTG010000218 DADXTG010000000
VERSION     DADXTG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.224"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWI57_002700"
     CDS             <1..888
                     /locus_tag="IWI57_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBC2450791.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(1373..2002)
                     /gene="udk"
                     /locus_tag="IWI57_002701"
     CDS             complement(1373..2002)
                     /gene="udk"
                     /locus_tag="IWI57_002701"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBC2450792.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT          733 a          374 c          357 g          654 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc attagggggt gcgacaaaag agcttcttca aaaaaataag tcgaaatctc
      961 acacaaattc gagaaataat tttcgtaagt ttcttcttat ttattgcatc tgaccacttc
     1021 tgttacaacc tctcgttaaa cggtgttcaa aagcttgcat ctacgataat aagcccaacc
     1081 caacgaaaaa cgtgaggagc tgttttattt ggatgctctt atcgcttgat gcaaagacag
     1141 cttttttaca acctctactt cacggtgctc aatcagctga ccttcggtat tagctcaaaa
     1201 aacggtaaaa tataaacagc tattttccgt ttcttctttt taatacttag gtcaagatgc
     1261 tgttttcaca acctcttgtt ataaataaaa aaacagttgg ctggtgactg ttcaaaaagc
     1321 agaatccttg cttttcaaac ggtcgcagtc cgttcaactg tttcgctgcg cactatttat
     1381 ggtttaagaa actagctact tttgttgtaa tcaaatcaat agcaacgtgg ttttctccgc
     1441 cttctggaac aatgatatcc gcatagcgct tcgttggttc gataaattga tgatacatcg
     1501 gtttaacaac tgtcaaatat tgttcgatca cggagtccaa tgtgcgtccc cgttcttcca
     1561 tatcccgttt gatacgacga atgatacgaa tgtcatcatc tgtatctacg tatactttga
     1621 tatccatcaa gtcacgcaga cgttcgtctt ctaaaatcag gattccttct agaatgatca
     1681 cttcttttgg ttcctgaatg atcgttgcct gacttcttgt atgcgctaca tagtcataca
     1741 cgggtttttc aatggcttta tagttcagta agtcgcctac atgctgaatc agcagatcat
     1801 tatcaaaggc aaatgggtga tcgtaatttg tgttcaatcg ttcttcaaaa ctcaaatggc
     1861 tctgatcctt gtaataagaa tcttgttcaa gcatcatgat ggaatggtcc gggaagttgt
     1921 tgaaaatcgc acggctgaca cttgtctttc cgcttccgga tccacccgtc acaccaatga
     1981 taattggctt gctttttgtc attgactaaa acctctttcg tatccgtttt ttcattctaa
     2041 tctattatag cgaaaaaaca cgaaaatgct atatttttct gaaaattaat ccttttcaat
     2101 ttccaataat gaataaag
//
LOCUS       DADXTG010000219         2104 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.225, whole genome shotgun sequence.
ACCESSION   DADXTG010000219 DADXTG010000000
VERSION     DADXTG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.225"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWI57_002702"
     CDS             169..1539
                     /locus_tag="IWI57_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HBC2450793.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWI57_002703"
     CDS             1560..1844
                     /locus_tag="IWI57_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450794.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DADXTG010000220         2076 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.226, whole genome shotgun sequence.
ACCESSION   DADXTG010000220 DADXTG010000000
VERSION     DADXTG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.226"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(117..707)
                     /locus_tag="IWI57_002704"
     CDS             complement(117..707)
                     /locus_tag="IWI57_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBC2450795.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="IWI57_002705"
     CDS             1230..1391
                     /locus_tag="IWI57_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBC2450796.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="IWI57_002706"
     CDS             1410..1904
                     /locus_tag="IWI57_002706"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBC2450797.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1897..2058
                     /locus_tag="IWI57_002707"
     CDS             1897..2058
                     /locus_tag="IWI57_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450798.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          746 a          330 c          356 g          644 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatcc
//
LOCUS       DADXTG010000221         2055 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.227, whole genome shotgun sequence.
ACCESSION   DADXTG010000221 DADXTG010000000
VERSION     DADXTG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.227"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..189)
                     /locus_tag="IWI57_002708"
     CDS             complement(<1..189)
                     /locus_tag="IWI57_002708"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2450799.1"
                     /translation="MKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKLMKQL
                     NLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="IWI57_002709"
     CDS             complement(330..584)
                     /locus_tag="IWI57_002709"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2450800.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="IWI57_002710"
     CDS             complement(777..1052)
                     /locus_tag="IWI57_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBC2450801.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="IWI57_002711"
     CDS             complement(1024..1977)
                     /locus_tag="IWI57_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBC2450802.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          625 a          375 c          296 g          759 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactc
//
LOCUS       DADXTG010000222         2040 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.228, whole genome shotgun sequence.
ACCESSION   DADXTG010000222 DADXTG010000000
VERSION     DADXTG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.228"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..>2040
                     /gene="acm"
                     /locus_tag="IWI57_002712"
     CDS             6..>2040
                     /gene="acm"
                     /locus_tag="IWI57_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBC2450803.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPK"
BASE COUNT          800 a          333 c          401 g          506 t
ORIGIN      
        1 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
       61 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
      121 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
      181 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
      241 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
      301 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
      361 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
      421 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
      481 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
      541 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
      601 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
      661 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
      721 aagagggaat ccaacaattt ttacaagatt tccccggcgc tacatttaat ttttcagtaa
      781 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
      841 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
      901 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
      961 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
     1021 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
     1081 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
     1141 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
     1201 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
     1261 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
     1321 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
     1381 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
     1441 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
     1501 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
     1561 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
     1621 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
     1681 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
     1741 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
     1801 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
     1861 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
     1921 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
     1981 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
//
LOCUS       DADXTG010000223         2040 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.229, whole genome shotgun sequence.
ACCESSION   DADXTG010000223 DADXTG010000000
VERSION     DADXTG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.229"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            111..1763
                     /locus_tag="IWI57_002713"
     CDS             111..1763
                     /locus_tag="IWI57_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBC2450804.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
BASE COUNT          556 a          404 c          530 g          550 t
ORIGIN      
        1 tataatactt tgggtggcgg cattagtggg gttatatatt atttgagctg atatattcat
       61 cctaacctta aaaataaatg agcagaattt taaaagattg gagagattct atggatcata
      121 atgcaaatta ttatcattgg cttacaaatg ccaatgcaga aattgtagag gaactgcggt
      181 catattccga gaaggacatt gaggactcct tctataaaaa tctgtccttt ggtaccggtg
      241 gtctgcgtgg caccattggt gcaggcacta atcgtatgaa cgtacacacc gtaggtaagg
      301 caagccaggg actgtcagac tatctgaata agacctatga aaccccttct gtcgtcgtgg
      361 gctatgattc ccgaatcaag tccgatgtat ttgcaaaggt tgccgcggac atctttgctg
      421 caaacggtat tcaggtgcat ctgtggccgg agctgctgcc tgtgcctacc gtatcctttg
      481 ctatccgtta tctgaaggct tctgccggtg ttatgatcac cgcatcccat aatccttcca
      541 agtataacgg atataaggtc tatggcgcag gcggctgcca gatcaccacc gaggctgccg
      601 ctgagattct ggctgaaatt gaaaagctgg atatttttgc agatgtgaaa atgggcggcg
      661 acgcttccag aatcgcttct atccagccag aggttctgac tgctttcatt gaagaagtaa
      721 agggccagtc ggttctgttt ggcgaagaag tggacaagaa tgttgctatc gtgtattctc
      781 ctttgaatgg taccggtctg gtgcctgtta cccgtactct ggcagaaatg ggctacagca
      841 atgtgaccgt tgtcgaagag cagcgtctgc cagacggtaa tttcccgacc tgtccttatc
      901 cgaatccgga aattaatgaa gctatgtctc tgggcatgga gtatgccaag aagtataatg
      961 ctgatctgct gctggctacc gaccctgact gcgaccgtgt gggtatcgcc gtgaagaatg
     1021 cagaagggga ttatgtcctg ctgtccggta acgaaactgg tctgttgctt ctggactaca
     1081 tatgctctca gagaattaag cacggcaaga tgcccactga ccctgtgatg gtcaagacca
     1141 tcgtgactat ggatctgggc gagcggatcg cttccaacta tggcgtgcgt actgtcgatg
     1201 tgttgaccgg cttcaagttc atcggcgaac agattggtat gctggaaaag gcaggcaagg
     1261 tggacagcta tattttcggc tttgaggaat cctacggcta tctatccggc agctatgtcc
     1321 gtgacaagga tggtgtcaac gccgcttatc tgatctgcga gatgttctct tactacaaga
     1381 ctcagggtat cagtctgttt gataagctga acgagattta tgctaagtat ggctactgtt
     1441 tgaacaccct gcattccttt gagtttgagg gtgcggcagg ttttaccaag atgcagaaaa
     1501 ttatggttga attccataaa ggtcttgata tgattggagg taagaaggtc attgaaacct
     1561 tggattacag cacgggactg aatggcctgc cgaagtctga tgttctcaaa tatctgctgg
     1621 agggcaactg ctctgtagtg gttcggcctt ccggcactga gccgaagctg aagacctata
     1681 tcagcgttag cgcagaaagc aaggaagctg ccgaagtaat ggaagctgtt atggctgctg
     1741 aactggctga aatgtttaag taatactatg aaaaagatct gagtgtcaat gtccgaagta
     1801 gcaacatcta tgtttaataa aaatttcctg ctttacggtt ctgtttacat aattgaggta
     1861 aaggggccga ctgtgtggat cgaaaaggca aagaagtgtt aacagagaca cagcagcctg
     1921 atgaagcacc gatcaaagca gtaagggaga aataagagat attagagttt taataatgta
     1981 ttcagcaata gagatgaaag gagtagaata tgaataaaac tattgaaata ataaaatgag
//
LOCUS       DADXTG010000224         2030 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.230, whole genome shotgun sequence.
ACCESSION   DADXTG010000224 DADXTG010000000
VERSION     DADXTG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.230"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IWI57_002714"
     CDS             complement(<1..425)
                     /locus_tag="IWI57_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBC2450805.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IWI57_002715"
     CDS             complement(534..1565)
                     /locus_tag="IWI57_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2450806.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          626 a          330 c          350 g          724 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt
//
LOCUS       DADXTG010000225         2030 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.231, whole genome shotgun sequence.
ACCESSION   DADXTG010000225 DADXTG010000000
VERSION     DADXTG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.231"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..137
                     /locus_tag="IWI57_002716"
     CDS             <1..137
                     /locus_tag="IWI57_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBC2450807.1"
                     /translation="CKEKISLSNAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK
                     "
     gene            134..604
                     /locus_tag="IWI57_002717"
     CDS             134..604
                     /locus_tag="IWI57_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBC2450808.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            732..1946
                     /locus_tag="IWI57_002718"
     CDS             732..1946
                     /locus_tag="IWI57_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBC2450809.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
BASE COUNT          738 a          306 c          412 g          574 t
ORIGIN      
        1 ggtgcaaaga aaaaattagc ctttctaatg cggaaatcac aagaaaagga aaaaactggt
       61 atataacgat tgataattgt attataacgg taaatgcgta cagttatacc gttatcactg
      121 ctcacaaggt aaaatgataa atttttctat aaaggaatta aacgcatttg cagttattgg
      181 gcaggaagta gaactgacta attatcaaaa aaagaatatt cagattagta cacagttttg
      241 gagaaagttt aacagtagtt tgaaaaaagc gtatctttca caatcaggaa attgggtaaa
      301 atatgctttt atggaaagaa gaaatggaaa actttattat ttctgttcta ttccccaaag
      361 aaccattacc ccagagggct ttctgtataa agaaataccg tcttataaat atttggttgt
      421 ggagcatatt ggtgctatgg ataaaatata tgaaacttat ggcaagattt atgaagaaat
      481 tattcctagt acatcgtata ttcctataaa agatattatc ctacactttg aaaaatacga
      541 ttatcgtttt cattggaata gtgataattc agttattgaa atttggatac ctattcaaga
      601 ttagaagcaa ttttatatcc atacacacaa agcagttagt cttaggattg actgcttttt
      661 ctatacagat ttttaatgac aggcaattat caattatggt agttgccttt tttgattgga
      721 ggaattgaag aatgaatgat aaaaacttta ttgaagaact aagacaaaag cgtgaggaat
      781 acggagtaac acaaacaagg cttgctgttg cctgtggtat cagccgtgaa tattacaacc
      841 gcattgaaaa aggaaaacag ccattgaatg acgaattaag agaaatcata gaaaaacaga
      901 ttgagcgttt caatccgcaa gaaccactat tcttattgat tgattacttt cgtgtccgct
      961 ttcctactac ggacgcattg gcgattatcc gtgatgtatt acaactgaaa tctaattaca
     1021 tgctttatga agattatgga aaatacggat atgaaagcaa atatgtactt ggcgacatca
     1081 atatcatgtg ttccatgcag gagcatttag gtgttttatt ggaactgaaa ggcaaaggct
     1141 gtcggcaaat ggaatgttat ctgctggcac aggaacgctc atggtatgac ttcatgctgg
     1201 attgtatgac ggctggtggt gtgatgaaac ggcttgattt ggctatcaat gataaagcag
     1261 gaatattgga tattccaaag ttaaaggaaa aatacaaggc tggcgaatgt atgtcctact
     1321 tccgtatgca gaaagattac agcggtacag aaaaatgcgg tagcgattta ccaaagaata
     1381 caggagaaac cttatatctc ggttcaacaa gtagcgaatt atatatgtgt gcttatcaga
     1441 aaaattatga gcagtatgtc aagaatggca cagaaattga agatacggag attaagaacc
     1501 gttttgaaat acgcatgaag aacgaacgag cctattatgc ggttgtagat ttactgacct
     1561 atcgggacgc tgaacgcacc gctttttcta tcatcaatca ttatgtccgc tttgttgaca
     1621 gagaggacga caagccaaaa agtcaatgga taacaaatga tgattgggca tggtttgtag
     1681 gggaaaacag agagccgata cgcttaacca caaagccaga gccttacact ttacaaaaag
     1741 cgttgcattg gctacaaaga caagtcgcac caaccataaa aatggtacag gcattagaca
     1801 gagaaaatcg tacaacgata ctgaaagata tgattgagca ggcagaacta aaagacaagc
     1861 ataaacattt attacaatta gagaaatcaa ccatagaaga acgtatagat accgctgttc
     1921 cacaagagaa tgacggtatt ttttaatgta attgaaaaat ttcagattta gtcagcaaag
     1981 agctgttttc attccctatg tgaagtgaaa gaagaaaaat aaatttttta
//
LOCUS       DADXTG010000226         2022 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.232, whole genome shotgun sequence.
ACCESSION   DADXTG010000226 DADXTG010000000
VERSION     DADXTG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.232"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            465..1283
                     /locus_tag="IWI57_002719"
     CDS             465..1283
                     /locus_tag="IWI57_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBC2450810.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1356..1715)
                     /locus_tag="IWI57_002720"
     CDS             complement(1356..1715)
                     /locus_tag="IWI57_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBC2450811.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          667 a          306 c          370 g          679 t
ORIGIN      
        1 ttagatggtt ttggtaaacc ataccctata acgcatttag taaaaaaata attcatgaat
       61 ggtttattac ctgattgaac atgaataaaa gtaatatttt tattttgatg gcctatttct
      121 aggttatgaa ttatccagat caaatttgtg tattacattc agtaataagg tgcttatctt
      181 aggatcaact tatgatacta gctgaccaag gatgtaccag ttatagaaaa tcagttaaag
      241 taaaaatgat aaaaatctta tgtcaacact gatttaggct gacctgtggg ttaggagaaa
      301 gacaggtcgg tagcttttaa tacaaaaaat ggcttgtatg tatctagata gttaactgaa
      361 attagaaatg ttcttatttt taacggagta agaaaaatag gctgctttag gtctgaatac
      421 tttttcaata taatgaagat atcaaaggcg acaggagtgt ttttatgaat aagaatcctc
      481 ataatctaaa gttggtagca gtagatatgg atggaacatt tgctcgtact gattacacat
      541 acgatatccc aaggttcaag gctgttttat cgcgaatgaa agctgttgat tgtcaatttg
      601 ttgttgcaag tggaaatcaa tactaccaat tgagatcgtt atttccaaat tattatgatg
      661 aattaagttt tgttgctgaa aatggtgctt ttgttaaaga taaaacagag gtactatttt
      721 cagccgatat gcctagaaaa gcgatcgagg tggtccttga tttatgtaaa gactatcctg
      781 aagtaaaaaa tgtgatgtgt ggtctgaata gtgcctactg tgaaagagga aaggtgagtc
      841 aagcattttt tgatctaacc aatatctatt accatagatt agaatgggta gctgattttg
      901 cagaagttca agataaaatt ttaaaatttg ctccgactat gcctgttgaa aaaaccgaat
      961 tttacgttga attgttccgt gaaaaattag ccgggctatt agtacctacc agtagtggtc
     1021 atggttctgt agacttgatt ctttctgagt gccataaaac tttcggcttg aaaatgttag
     1081 ttgagcgctt ggggattaat cctgaccagt gcgttgcttt tggagatggg ggcaatgaca
     1141 ttgagatgtt ggagtattgt ggtctgagtt atgaaatgga taatgcaacg gaagctgtta
     1201 agcaagttgc taagcatcaa tgtccttcaa atgacgaaga cggtgtttta gtgacgctcg
     1261 atcgattgtt ccctaacacg taaacactag gtaattcatc aaaaaacctc gatataagaa
     1321 atattcgagg ttttaaaact atatttagaa cagatttacg aagcgtctga tgagtcaaaa
     1381 tagacttgga cattgctatc taaaatacca ctttcattct ttaaacgaat tgtttgattt
     1441 gatggagtta cctctaatac taaatgccca ttaataaagg tagtatcgta ttcagatgtt
     1501 ttaactgttg cttcttcgca aggtattgtt aaatcatatg ctggaatata atgttgtgtg
     1561 gaaactaata aaaaatgatc accaatcata cagcatccct ctgctgaaga aaattgatta
     1621 gagccatcat ttaaagttaa caagaatcca tcctttaaac gaggtacttg cttaaatact
     1681 tctggttgta cttctaaaaa taaattatcg ctcattttca cgaccaccat cgacttccac
     1741 agttgaaatt gacattttat ttaacacacc tattccccct taactataac tcaattctat
     1801 ataaaaaata ttctattctc aaataattga acttttcaaa tcgccaattt acttcggctt
     1861 tatcagagct cattcatatc aagagcgatt aacgctgtaa aaattaaacc gaagatctga
     1921 taaattttct ctattttcta caatatttaa aacgcttcct tagtctatta tatatctatt
     1981 tctcttagga gtatgtttag atgtttgaat aatagttgcc ca
//
LOCUS       DADXTG010000227         1978 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.233, whole genome shotgun sequence.
ACCESSION   DADXTG010000227 DADXTG010000000
VERSION     DADXTG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.233"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..295)
                     /locus_tag="IWI57_002721"
     CDS             complement(<1..295)
                     /locus_tag="IWI57_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBC2450812.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTT"
     gene            complement(306..974)
                     /locus_tag="IWI57_002722"
     CDS             complement(306..974)
                     /locus_tag="IWI57_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBC2450813.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1104..1688)
                     /locus_tag="IWI57_002723"
     CDS             complement(1104..1688)
                     /locus_tag="IWI57_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2450814.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          712 a          333 c          265 g          668 t
ORIGIN      
        1 ttgttgtaaa gaattgtcct ctaggatctt gacttacgta gccaatattt tttgatatac
       61 tttcttgtgt taaggattgt agcgagttat tttcaaataa aatttcccca accaattttc
      121 cgtcataaag tcttggaata agtccattca ttaactttaa tagtgtggat ttcccactcc
      181 cagaagaacc agtaatcaat gtaagactgc ctttttttat tgaaaaatta aggtcaacaa
      241 gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt gttaaattca aacaattctt
      301 tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg aaaacaggat gatatagtct
      361 aataagctca tgtttgtttt atagtaagaa cttcttttca caggagattc tatccccttt
      421 gttattgcat tagcggtcat ttcttcagca atcatagttg cacgtataag aacgggaact
      481 aacagaaatt ccaaatattg cagaggctga aaaaaagaca agcgaatgtt tctcattttt
      541 aaagattctt taatcattct tatttcagaa cgataagtcg gaaagaatct gatcactact
      601 gtaatactta acattatttt tttcggtaaa tgtatttttt ctaaagagga taataattca
      661 ttgctagtat agacactaac caacaaccat gagatcatta cgaccggcaa cattcgaaat
      721 acaatatata aggatgtcgc aaaaaatata gtaaaaaact ctatcttgat agcatcaata
      781 attttcatta ctaaaaacag aactgaataa atcaagatga aataaataca tgtctttatt
      841 ttacgaaaca tcaatactaa aactgtagca ataatgaaac accccacttg aaatatattt
      901 ccctttccta aaaaaataat gaccggaaaa aaaatattta aagtaatcaa ggttaacgga
      961 ttgatttttg acactctttc acctccaaaa aataagatcg cactgttatc ttaagctgat
     1021 aaaaatgcac cgttattcag ataacagtgc gatcttatca aaaagcataa ctgagaacaa
     1081 ttctcaatgc aagctttttt taattaactc agtaattcaa tccacccagg aacagaaaat
     1141 tgatacaatc ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata
     1201 tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact
     1261 aaattttgat tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttc
     1321 gtcacaaatg atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat
     1381 aaaatcaaat cgaatatacg tttattctca aaaatataat taataaatgg atctaattca
     1441 ttgaagttct ctctccaact tttctctata tctatatttt ctaagtttaa gaggtatttg
     1501 gtaaactctt cattatacat attaataagc gcattaatat gcggctgaat aatttcttca
     1561 aataacgctt ccttagaagt aaaatgttta taaaaagcac ccgtagttac gcctgcggtc
     1621 aggcacagtt gccttatctt agtttgctga aatccttctt tttcaaaatg gcttagtcca
     1681 ctttctatta acttttggtg agttataata taatctctgg tttctatggg tacttcctcc
     1741 taataagatg aattaacttt attatcttac acaggacttg aagtttcaat cgttaacgtt
     1801 tattactgaa ctcattcctt agagctaaac taatgagaaa gagcagaata actgatacaa
     1861 aaagcaatcc tacaagaatt aatagactca tctaaatcat tcaccttttc taaaataatc
     1921 cgagaaagat ttaaaaaaat tatatccagc tattaggata agtcctatca taatcact
//
LOCUS       DADXTG010000228         1943 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.234, whole genome shotgun sequence.
ACCESSION   DADXTG010000228 DADXTG010000000
VERSION     DADXTG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.234"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..173
                     /locus_tag="IWI57_002724"
     CDS             <1..173
                     /locus_tag="IWI57_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBC2450815.1"
                     /translation="RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSV
                     CTEIKVLLGIPA"
     gene            227..370
                     /locus_tag="IWI57_002725"
                     /pseudo
     CDS             227..370
                     /locus_tag="IWI57_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
     gene            468..677
                     /locus_tag="IWI57_002726"
     CDS             468..677
                     /locus_tag="IWI57_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBC2450816.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            694..966
                     /locus_tag="IWI57_002727"
     CDS             694..966
                     /locus_tag="IWI57_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBC2450817.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            968..1831
                     /locus_tag="IWI57_002728"
     CDS             968..1831
                     /locus_tag="IWI57_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBC2450818.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT          768 a          317 c          358 g          500 t
ORIGIN      
        1 atcgtccagt aaagagacgc aataaattct atcgaagttt acgcactgcc tctaccacga
       61 ttaaaggcat ggaagccatt cgaggattat ataagaaaac ccgaaaagaa ggcactctct
      121 tcgggttttc ggtctgtact gaaatcaagg tattattggg aatcccagct taaatcatag
      181 ataccgtaag ggattttatt ctttatttaa aactttgcaa cagaaccagc gaagtaataa
      241 agtaagtacc tggtctaaaa acggcattac agagcataaa ggctatgaca aaaaagtttt
      301 atccatgtat aagaacgtat tttttgaaat gcttgagcga attattcaat tagaaaacga
      361 aaaagaatag aatcacaaat cacaagtgat taatcacaaa tcacttgtga tttgtgattg
      421 ttgatgataa aataagagta agaagaaata gaaagaagtg agtgattgtg ggaaatttag
      481 gcgcacaaaa agcaaaacga aatgatacac caatcagtgc aaaaaaagat ataatgggag
      541 ataagacggt tcgtgttcgt gctgacttgc accatatcat aaaaatcgaa acagcaaaga
      601 atggcggaaa cgtaaaagaa gttatggatc aagccttaga agaatatata cggaaatatt
      661 tacctgacaa actttaaaag gagtgaaaat gaaatggcag ttacgtatga aaaaacattt
      721 gaaatagaga tcattaacga attatcggca agcgtttata atcgagtatt aaactatgtt
      781 ttgaaccatg aattaaataa aaatgactct caattattgg aagtcaattt attaaaccaa
      841 ttaaagcttg caaaacgtgt aaatcttttt gattattctt tagaagaatt acaagccgtt
      901 catgagtatt ggcggtcaat gaatcgttac tcaaaacaag ttttgaataa agagaaagtg
      961 gcttaatatg gcaaatatag tcaattttac tgacaaacaa tttgagaatc gcttaaatga
     1021 taatttagaa gaattgattc aaggaaaaaa agcggttgaa tcgccaaccg cttttttact
     1081 tggtgggcaa ccagggtcag ggaaaaccag tttgcgatca gcaatttttg aagaaacaca
     1141 agggaatgtt attgtcattg ataatgatac ctttaaacaa cagcacccta attttgatga
     1201 actagtgaaa ctttatgaaa aagacgtagt aaaacacgtt accccttatt ctaatcgcat
     1261 gacagaagcg atcataagcc gtttgagcga tcaagggtat aatttggtga tcgaaggtac
     1321 aggacgaaca acagacgttc ctattcaaac cgcaacaatg cttcaagcca aaggttatga
     1381 aacaaaaatg tatgtcatgg cagtacctaa aatcaactca tatttaggaa caattgaacg
     1441 atatgaaacc atgtatgcag atgatccaat gacagccagg gcaacaccaa aacaagcgca
     1501 tgatattgtt gtcaaaaact taccgaccaa tttagaaacc cttcataaaa cgggcttatt
     1561 tagcgatata aggctatata acagagaagg agtaaaactc tattcaagtt tggaaacacc
     1621 ttctattagt ccaaaagaaa ccttagaaaa agaattgaat cgtaaagtat cagggaaaga
     1681 aattcaaccg actttagaac gaatagagca aaaaatggtt ctaaataaac accaagagac
     1741 acctgaattt aaagcaattc aacaaaaatt ggaaagcttg cagccaccta caccaccaat
     1801 acccaaaaca cctaaacttc caggtattta aaccttaaaa aaggaaaaga gtagttacca
     1861 aaaaacggta actactcttt ttttataaaa acatttcctc atgttggggt cccgagcgcc
     1921 tacgaggaat ttgtatcgat aag
//
LOCUS       DADXTG010000229         1921 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.235, whole genome shotgun sequence.
ACCESSION   DADXTG010000229 DADXTG010000000
VERSION     DADXTG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.235"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..211
                     /locus_tag="IWI57_002729"
     CDS             <1..211
                     /locus_tag="IWI57_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450819.1"
                     /translation="YEELMTTDEIERSYENDKMYIIPPHYHATDELYKDYEKIKITAY
                     SSDATELMSGQEFIRIAKENNVVTW"
     gene            223..1227
                     /gene="neuB"
                     /locus_tag="IWI57_002730"
     CDS             223..1227
                     /gene="neuB"
                     /locus_tag="IWI57_002730"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBC2450820.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1224..>1921
                     /gene="neuC"
                     /locus_tag="IWI57_002731"
     CDS             1224..>1921
                     /gene="neuC"
                     /locus_tag="IWI57_002731"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBC2450821.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIEEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQS"
BASE COUNT          700 a          249 c          379 g          593 t
ORIGIN      
        1 atatgaagag ctaatgacta cagacgaaat tgaacgctca tatgaaaatg ataaaatgta
       61 tatcattcca ccacactatc atgctactga cgaactgtac aaagattatg aaaaaattaa
      121 aatcactgca tactcttcag atgcaacaga attaatgagt ggacaggaat ttattagaat
      181 cgcaaaagag aataatgtcg tgacttggta aggagtttta tgatgaaatc agaagtatat
      241 gtaattgcag aaattggtgt aaaccataat ggaagtctag atttagcttt agaaagtatt
      301 gataaagcta aagaatgtgg agctaacgcg gttaaatttc aaacatttaa aacgaataag
      361 ctagtttcca aaaaggctcc aatggcaaaa tatcagaagg ataatcttaa agaggacagt
      421 tctcaatttg aaatgctaaa aaaattagaa ttatctcaat ctgattttat tagaataaaa
      481 gattattgta tacaaaataa aattgatttt ttgtctactc cttttgatga agaaagtgca
      541 gactttctaa aacaaattgg tgtacaagga tttaaaattg gctcaggaga tttaacaaat
      601 ttaccttttc ttagaaaaat agatatgtat gggttgccag ttttattatc tacaggtatg
      661 gctgatattc atgaggtcag tgaggctgta gattgtttca tagatagtcc agttactatt
      721 ttacattgta cttcaaatta tccggctagt tctgaagata ttaatttatt ggcgattaac
      781 acattaaaag aaatgttcgg gatacccgtt gggtactccg atcattcatt gggatatgat
      841 gttggtattt gtgctgttgc gatgggtgct aaggtaattg agaaacattt taccttggat
      901 aaggaactac ctggtccaga tcataaagct tctttagatc ctcaggaatt tgcagatttt
      961 gtaaagcata ttagaaacac tgaaatattt ttaggtgatg gtgttaagcg agcaatgcct
     1021 tctgaactta gtacaaaaga agtatcaaga aaaagcctag tggtcactga gaatttaaat
     1081 gtgggagata ttcttacaga gaaatctctt acaataaaac gtcctggaaa tggaatagct
     1141 cctaagtttt tatatcaata tattggcaaa aaagttaaaa gaccaataag tgcagatagt
     1201 gttctgcttg aggaagatgt tgaatgaaaa aaatacttat agtaagtgga acacgagctg
     1261 attatggaat atataaacct gttgctgaga atttactaca agataattgg gatgtatcat
     1321 ttgttgtttc tggaatgcat ttatcccata aatatggatt tactaaagaa ttgatcttag
     1381 ctgacggatt tagaattagt ggagaagttc aaagtttgtt tttagagaac aataagggaa
     1441 acatggctcg gtctgtggca ttagagataa atggtttcac aaatatattt gaacaaacta
     1501 gccctgattt tgtattatta ctaggggata gaggagaaat gttggcagct gcgatagctt
     1561 gtttgtatct tggaatcaaa acagcacata ttcacggagg cgaagtgtct ggtactgtag
     1621 atgaatcaat aagacatgcc ataacgaaaa tagcatctgt tcattttgct gctacggaaa
     1681 aaagcaaaga gcgtttaata aaaatggggg aagattcttg gagagttttt cccgtcggag
     1741 ctcctagaat agatactatt ttaaataata gattacctag ttttagagat atagaagaaa
     1801 aatatcatct aaaatttgaa aagaaaaaat acgttttgca agtatttcat ccagttgtaa
     1861 ccgaactaga aaatatagaa aaacaaattt tagctttgat caattcaatg gaacaacaat
     1921 c
//
LOCUS       DADXTG010000230         1919 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.236, whole genome shotgun sequence.
ACCESSION   DADXTG010000230 DADXTG010000000
VERSION     DADXTG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.236"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            640..972
                     /locus_tag="IWI57_002732"
     CDS             640..972
                     /locus_tag="IWI57_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBC2450822.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1563..1892
                     /locus_tag="IWI57_002733"
     CDS             1563..1892
                     /locus_tag="IWI57_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450823.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          654 a          272 c          399 g          594 t
ORIGIN      
        1 agggaactag tgtttgcata tgtgcttttt tgagttataa taattcatga agagaggtaa
       61 ttcacttatt tccccaagct gaaaagcctg atattacttc ttttcaggca gtgctgctaa
      121 tattcaatta cacttttttg tatattttta gttgttttaa atttaattgt ctgctatgct
      181 tgtgatgaat tgattttcgt tctgcacttt tggacgataa atttggacaa ttgaaaatac
      241 aaaaaaaaga aaggccctgt gagactcgga aggctggtaa ccttcgtcga cgtccacagg
      301 tggcattaag tctatacccc ttaatgcgtt tacctttcag tgttacggta ttacccgtaa
      361 cggatttagt ataacagata ttcaaaagtg ataccacttt ttttggctgt gttaaggtta
      421 aaaatacgtg atattacgat gaatctatgc ctaagcataa tgttataaaa atgtccgtgg
      481 ggctctttgc cgtatgacga acgctgaaat tagagggaac gcatgtttga atgcgttccc
      541 tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa
      601 cttgttaaaa atatttaaag taagtgggtg gattatttta tgtcatatac aggaattttg
      661 agtctcgaag atatttgtca ctacggtaaa cggtgtaccg ctacagaaaa gatcacgaag
      721 aaactatcaa ctggtcaaag taaaaccgtt gtgcagtgta agaaatatat aatccagaaa
      781 gacaaagtct cagaagaaat gatttattac attgggaaac aaaaacaaat aattttaaaa
      841 gatcctatcc ctttaaaaga gctatatcca acaatcaaac atgtttatga ccaaaacggt
      901 gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt
      961 ttaatcagct gaattgatca ttctcataac ttgtatttta tcgtttttcg cttgcttgaa
     1021 aatattgtcg agacttctaa aggaacttag aatcactaga aaaataagga gtagacttga
     1081 tctatcaaca attcccttcg gggagtgacg gggtaggttt ttacgattgg taacgtaaaa
     1141 aattggtcaa tcgagtaaat gcccttgttt ggatagtggt gaaatgaatt tgtgtaaata
     1201 atatgcacag agtaaacaag agggaattta gaagttagac aagttagaat agcgcctaga
     1261 ttaatcatga gcagctacta agggatctag gctttgacgc aaactcatgg aataaggagt
     1321 aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt cgtgtgctgc agtacatgta
     1381 gatacttgcg aggacgttga tcattggatc aatcgagcat ttaccgctgt ctactagggt
     1441 ttctcgtttc ggacgccata aacgagtcga tttattttat atatttttga gtgaacgaaa
     1501 aacgataaag atattcgtta tgaagtaaaa ctaatttagt agatagcaaa ggtggtagag
     1561 gaatgattca aaaatttgcg aaaatgagta aaactgcatg gattgaaaag cttgaaaatg
     1621 acttgagtgt catgcgatta gctccaaaga aattcaaacg agagattgca gaaaaagaag
     1681 caattttgtc ggtattgaat gcggtagacg ataaatgtcc agtaaaaatt agtttttcag
     1741 atatagcaac aattatcgaa ggtatttatt atggtgaagt tgatgaagag atatcttcta
     1801 caaaagattt attagatttt ttggataaaa acaaagatac ctttatagta ctaactgatt
     1861 atgaggaggt tacaacaaat gaaaaattat gatccgaata tacgattggg cacacatac
//
LOCUS       DADXTG010000231         1868 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.237, whole genome shotgun sequence.
ACCESSION   DADXTG010000231 DADXTG010000000
VERSION     DADXTG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.237"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..83
                     /locus_tag="IWI57_002734"
     CDS             <1..83
                     /locus_tag="IWI57_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBC2450824.1"
                     /translation="AYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            192..875
                     /locus_tag="IWI57_002735"
     CDS             192..875
                     /locus_tag="IWI57_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBC2450825.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            1022..1636
                     /locus_tag="IWI57_002736"
     CDS             1022..1636
                     /locus_tag="IWI57_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBC2450826.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            1652..1858
                     /locus_tag="IWI57_002737"
     CDS             1652..1858
                     /locus_tag="IWI57_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450827.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT          680 a          301 c          383 g          504 t
ORIGIN      
        1 atgcttataa agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag
       61 aatttgaagt aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt
      121 ctcctgaata gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg
      181 gaggagatat tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag
      241 ctttggaaaa cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa
      301 aagaaggcgg agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc
      361 gaatcgttga tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt
      421 ttattactgc gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg
      481 cactggatgc aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa
      541 tgattcacga acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc
      601 aagcgcaaga agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt
      661 cacgaaagac gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata
      721 tcccagtaat tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat
      781 taggtgcttt ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa
      841 gatatgtcga agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg
      901 acaaaaaaga aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt
      961 acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac
     1021 catgtttgaa cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg
     1081 ttcagtcatc cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa
     1141 tatcgcaccc ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc
     1201 gatccagaga aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga
     1261 agctgttatc catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa
     1321 tctgcctaat gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat
     1381 catttcagta ctagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca
     1441 tgtcgagata cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa
     1501 atatcattta aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt
     1561 gaatgctgta agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat
     1621 tgcacgtccc gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac
     1681 agattcaaaa gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta
     1741 cttagaagtt ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa
     1801 ggaaatagaa atgccagatc aaacgattag cggattgttt aattacttta acaaataaat
     1861 atttattc
//
LOCUS       DADXTG010000232         1857 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.238, whole genome shotgun sequence.
ACCESSION   DADXTG010000232 DADXTG010000000
VERSION     DADXTG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.238"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            108..1721
                     /locus_tag="IWI57_002738"
     CDS             108..1721
                     /locus_tag="IWI57_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBC2450828.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          589 a          331 c          390 g          547 t
ORIGIN      
        1 tctctatatt ttctgtctat tcaaaataaa caataatttt ccggcacaat tatttgctgt
       61 tttttaccat aacatagtac gatcaacgta gaaaaggaga tgtttttatg aaagttgttg
      121 tcattggcgg tgtagctggc ggtccttcat ttgccactcg tttccgtcga ttgaatgaag
      181 cgcacgaaat cattatctat gaacgcggag aaaatatttc ttatgcaagt tgtgctttgc
      241 cttattattt aggaggtgtg atcactgacc gtgactcgct gatcgaacgt acaccagaaa
      301 tattgaaaac aaaaaacaac atcgatgtct ttactaaaca cgaagtaaca gcaatcgatc
      361 cttctactaa gcgattaaca gttaaagacc tatccacaaa tgaagaaata aaaacagatt
      421 acgataagtt gatcatctct tctggtgcta gaccagatta tccggatatt cccggagttt
      481 ttgaagcaga aaacggcttt gtcctccgta gtgtgacgga tgcggatcga atcaaatcgt
      541 tccttgaaga aaaaaatcca caacatgtcg tcattcttgg tgcaggtgtt atgggtctgg
      601 aattagctga gaatttcaag catcgcggct tagacgtgac tttaatcgat caattgccac
      661 aagtcgcttt cccttatgat ccagaaattg ctaatttagt ttatgacaaa ttgctgaaag
      721 aaggattagc cgttcattta gaaacaagag ttactgagat ccgtgataaa ggtcgagaaa
      781 tagtattatc agatggctcc gtcctttctg ctgatatgct aatttttgct gttggtgttt
      841 ctccgaagaa tgaagtggtg aaagcaaccg gcatacaatt atcagataca ggtcagatca
      901 ttgttgatga ccagttacaa accaatctgc cggacatcta tgcgattggc gatattatcg
      961 aaacgactag tgtagtgact ggtcagccga tccaaagtat gctttccagt gcggccaatc
     1021 gtcaaggaca tatgctggca gatattttaa atggtacgcc tatgcgctat cgcggatata
     1081 ttggtgcagg tgtcgcaaaa atctttgatc atacagcaag ttatgcggga atgacagaac
     1141 atgaattaaa agcatcaggc ataacaaatt ataaaactgt ttttatcact ccctttgacc
     1201 atgcctattt ctatccagaa gctacaagat taaatctaaa gctgattttt gatgcagata
     1261 gcggtcgtat tttaggtgga caagcatttg gagaaaaagg tgtcgataaa cggatgggag
     1321 aactttctgt agcaatcacc ggaaacttga cagtctttga tttgcctgat ttggagttgc
     1381 cttactctcc accgtattct actacccgtg acccgttgaa tatagctggt tatgtcgcaa
     1441 tcaatcaaat gacgaatatc gtagaaacga tcaaagcaag tgatataccc gaaaacgatt
     1501 tgaaagaagc gttcttttta gatatacgtg aacctaataa agcaccttct ggcagtatct
     1561 cggcaacaaa aaatattcca atgaatgagt tacgagatcg aatcaatgaa atcccaaaag
     1621 ataaaaaagt ttacattact ttcagaagag gattgaatac ttatacttct gcccgaatct
     1681 tggcaggttt gggtatcaaa gcgattttga ttgaagaata aaaaaacgac cgcggtgggt
     1741 agccgcggtc taggagttat ttttacttga ggagtaaaaa tgaaaaagta tatagggttg
     1801 ttggttggta tgtatataga atagaagata aatgtgaaga aattgtgcat tatttct
//
LOCUS       DADXTG010000233         1817 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.240, whole genome shotgun sequence.
ACCESSION   DADXTG010000233 DADXTG010000000
VERSION     DADXTG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.240"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(124..>1817)
                     /gene="uidA"
                     /locus_tag="IWI57_002739"
     CDS             complement(124..>1817)
                     /gene="uidA"
                     /locus_tag="IWI57_002739"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBC2450829.1"
                     /translation="EKESYPISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEE
                     RLVLRFGSATHEAKVFLNGKFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDS
                     TLPVGIMKETVQENGDIKKENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITD
                     LDASVEFNIDTEWTGKILIAIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMK
                     ISLIDDNKVIDTYTEEFGIRSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAV
                     NIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDD
                     NVEKHNTWKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLV
                     ELTKELDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLK
                     KELDGWMERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENF
                     VGEQVWNFADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          579 a          368 c          218 g          652 t
ORIGIN      
        1 aaaaaactag aaaaaaaaca taaaaatatt tatccttctt taaacagaaa atctttctat
       61 aaaaaacaaa aaaaacgatt aggctaggct aatcgttttt tgaacattct taaaaaatcc
      121 aaattatttt ttataactga aattgggtat tgatgaccat cgtttctgta gatagtaagc
      181 aatcatttta ggttttctat ctctagtaaa gattcctttc ttatttcctt gcactcttaa
      241 aatcccttga cttgttgcaa aatctgcaaa attccacact tgttcaccaa caaaattttc
      301 acatgtatct attacttcgt gatttgcttc gtaataagcc acttgatact cttctgtaaa
      361 cattgttggt actgtatcat gaaatccagc tactgtatct gctccatatt ctgtaaacat
      421 tattggcgta tcaggacatc tttccatcca tccatctagc tcttttttca acagatattt
      481 tgcatgtgcc agatcccctc ccgaaacata ccaaccataa taacgattta aacataaaac
      541 atcgacaata tctcctacct tacaaacatc tggcgttgat tttagataag taacgattgt
      601 tacaggtctt ttctgcggat caagttcttt agttaattca actaaaggtt caaaatattc
      661 tttagcacct tcggaatccg aatctggttc atttgcaata gaccacatta caacacttgg
      721 gtggttcttg tcgcgttcta tcaattcctt gatcaccgtt ttatgagaat tttctgttcg
      781 tatctctttc cacgtattat gtttttccac attatcatct aataaagtgg ccatgaaatt
      841 taaatgtaac cctactgcag gtacttcatc aataactaca ataccttctc gatctgctaa
      901 tcgcataatt tcttctgagt aaggataatg tgatgttcta aaagaattag cacctatcca
      961 tttcattaaa ttaaaatctt taatatttac agcctcattc aaaccacgtc cattaacata
     1021 cgaatcctca tgcttaccaa atcctttaaa ataaaaaggt ttattattaa tcaaaaattt
     1081 gccattggta acctgaacac tgcgaatacc aaattcttct gtataggtat cgatcacttt
     1141 attatcatca attaaactaa ttttcatact atataaataa gcgtctagag gttcccaaaa
     1201 aattggattc ttaagctgaa tcattccttt ctttccttca gcttcagcta caagttgagc
     1261 ctcttcgtca tatatcgcta ttaagatttt tccggtccat tctgtatcga tattgaattc
     1321 tacacttgca tccaaatcag ttattcttgt attaatagta atatcactta tatagtcttt
     1381 cggagttgta tataacttaa caggtctatg aatacctgca taattgaaga aatcaaaatt
     1441 aactaagttt tcctttttta tatccccatt ttcctgtact gtttccttca taatccccac
     1501 aggtaaagta ctatcgtcaa taatattgtt tacagcaact gtaattcgat tattcccgtc
     1561 ttttacaaac cgattaattt cagactcaaa tggtgtaaac ccacctttgt gttgagtaat
     1621 aaactttcca ttcaaaaaaa cttttgcttc atgtgttgca gatccaaaac gaagaactaa
     1681 tctttcttct aataatcttt taggaagagt cacttctttt tcataccaca cccatccaac
     1741 atgatttcta atgttctctg tttcaaataa atcattataa gaagagggaa ctgaaatcgg
     1801 ataactctct ttttcat
//
LOCUS       DADXTG010000234         1807 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.241, whole genome shotgun sequence.
ACCESSION   DADXTG010000234 DADXTG010000000
VERSION     DADXTG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.241"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IWI57_002740"
     CDS             287..694
                     /locus_tag="IWI57_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450830.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IWI57_002741"
     CDS             708..1109
                     /locus_tag="IWI57_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450831.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IWI57_002742"
     CDS             1111..1482
                     /locus_tag="IWI57_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450832.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..>1807)
                     /locus_tag="IWI57_002743"
     CDS             complement(1518..>1807)
                     /locus_tag="IWI57_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450833.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          673 a          236 c          307 g          591 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtctcat
//
LOCUS       DADXTG010000235         1796 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.242, whole genome shotgun sequence.
ACCESSION   DADXTG010000235 DADXTG010000000
VERSION     DADXTG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.242"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            42..698
                     /locus_tag="IWI57_002744"
     CDS             42..698
                     /locus_tag="IWI57_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBC2450834.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            880..1410
                     /locus_tag="IWI57_002745"
     CDS             880..1410
                     /locus_tag="IWI57_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2450835.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT          651 a          248 c          368 g          529 t
ORIGIN      
        1 tgtatggggc gataaaatca gaatttgaga gaggattgaa tatgaataag gaatggtctg
       61 aacttaataa aacaatgcag gcacaaataa aaaagaaaga tacttataag aggggtattg
      121 atactttact tactttacga agtcagttaa tacaaacctt agtatctttc aaagaagaat
      181 tatgcagaga agattttaac tcaatcccct tcataaacgc tgatggttat catagtaaga
      241 cgattgctta ttctatttgg catattttcc gcattgaaga tatagttgtg catacagtga
      301 taaatgaaga tgaacaagta ttttttgcag gcaattatca agagcgtatc aattcaccta
      361 tcattacaac aggaaatgaa ctcatgaaac agcagattgc agacttttca aaacagctta
      421 atctggaaga attatattta tatattttcg aagtatggga aagcactgag aaaatgcttg
      481 aacggttatc ttatgatgaa ttgaaaagaa aaataccgaa agaaagaaaa ggatacttag
      541 aatcgttgaa tgtagtaaac gacaatgaaa aagcaatttg gctgattgat tattggtgta
      601 ataaagatat ttgtggacta attcaaatgc cgttttcaag acattggatt atgcacacag
      661 aagcctgttt gcgtataaag aacaagatac attcataggc aaaaatttca gtttgtagaa
      721 ctaaggaaat tggaatttaa ggaggaataa agtatggtta gtgattttat aagagcagca
      781 tttccatgga ttttaatggg tttatttgta gcaatcagtt gttcccttat gagtaaaaaa
      841 gagaaataaa ttcctgtttg tatttataaa agagaaaaaa tgagaaaagt agttttattt
      901 attgccatga gccttgacgg atatattgcg gacggtaatg gtggagtagc ttggctaaat
      961 ggtcatggaa atgacaatga aaacatcgac acttatacag aatttaccaa agatatagat
     1021 actgtcttaa tggggtggaa tacttctcat caggttgtta ctgaactttc tccgcaagaa
     1081 tgggtatata ataaatttac aacttatgta ttaacacata aagagtgcaa ttcttcccaa
     1141 caaattcatt ttacaagtga aaatcctgtt ttattattgg aacggctaaa acaagaagca
     1201 ggaaaggata tttggatttg tggcggagca aatcttgttc agcagttggt aagcaaaaat
     1261 atgattgata aatattacat ttctgttatt cctactctgt taggaaatgg ggttcgtctt
     1321 ttgggcaaca tagacaggga aataaagtta aggctttgta aaacacagac ttataacgga
     1381 ataaccgatt tgatttatat gcgtagataa ctagagagaa acagaaaagc tgttaaaccg
     1441 acggagcgat ccgaccacgt tggcgggaaa atccttaaga tttttccgcc atgcttgcaa
     1501 gggggcggtt cgcttcggcg gttcggcgag cttgcgagcc gttcaaagcc cccgttatct
     1561 aacaaggggg gcacaaaaaa caagaaacac tatacactaa caagcggaaa acgctgtatt
     1621 tataaggcaa aaccccattt ttacagtagt gatataaatt gtacctatat cactatgaaa
     1681 attgaatagt aaatatgaga aagacagttg atttcaaaaa ataagaaatt gattgtcgtt
     1741 ttcttttgca gaaatttaag tgcaggcaat tatggtagtt gtcgtaattg ctgtca
//
LOCUS       DADXTG010000236         1732 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.243, whole genome shotgun sequence.
ACCESSION   DADXTG010000236 DADXTG010000000
VERSION     DADXTG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.243"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..1169)
                     /locus_tag="IWI57_002746"
     CDS             complement(150..1169)
                     /locus_tag="IWI57_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBC2450836.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1180..1386)
                     /locus_tag="IWI57_002747"
     CDS             complement(1180..1386)
                     /locus_tag="IWI57_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBC2450837.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1519..>1732)
                     /locus_tag="IWI57_002748"
     CDS             complement(1519..>1732)
                     /locus_tag="IWI57_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBC2450838.1"
                     /translation="PSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIP
                     RKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          494 a          352 c          291 g          595 t
ORIGIN      
        1 gaatgtgtgg caacaaacct gttatcgcat agcttggagt gtgactatta tgggtgatag
       61 tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt caatatagtt
      121 atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa ataccaacgt
      181 ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt cgttgcccgc
      241 atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac atgatagcca
      301 acatttccag aagtattata agcttgattt acatctgatc tataaattcc atcagctctt
      361 actcgagcta attctttccc agtattgtaa tccataatga agatatactc gtatttatag
      421 ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc aaactgatca
      481 atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc tcgtgttgga
      541 tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc atgagctaaa
      601 tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta aggatctgta
      661 tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg ttggttataa
      721 ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc caattcaacg
      781 tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa ctcaatctgc
      841 acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc aggttgaccg
      901 acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct agtccatgaa
      961 cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt ttcatgcagt
     1021 aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa attaaattca
     1081 ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt taacaggctc
     1141 atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg atgaaatttt
     1201 cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa aacctgtaat
     1261 aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa gaccgacaaa
     1321 agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa aggttctatt
     1381 gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat cgcttgtttt
     1441 cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc agttgttcaa
     1501 ttttatcttt cttaattgtc aaattaagtt agacagatca tcaatactta cgtaactcaa
     1561 aaatacttcc aaaggtgttc gataatccag tgattttcta ggaatattat ttcgtttaga
     1621 tgcgacagat tgaataaaaa attcatctac tgaattgaaa tccatagatt tcaataaacc
     1681 atctctacgt aacaatccgt tagaattctc gtttaggcct ctttgagacg gc
//
LOCUS       DADXTG010000237         1651 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.244, whole genome shotgun sequence.
ACCESSION   DADXTG010000237 DADXTG010000000
VERSION     DADXTG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.244"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..1368
                     /locus_tag="IWI57_002749"
     CDS             34..1368
                     /locus_tag="IWI57_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450839.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT          568 a          236 c          285 g          562 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttact ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt a
//
LOCUS       DADXTG010000238         1633 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.245, whole genome shotgun sequence.
ACCESSION   DADXTG010000238 DADXTG010000000
VERSION     DADXTG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.245"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            203..643
                     /locus_tag="IWI57_002750"
     CDS             203..643
                     /locus_tag="IWI57_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2450840.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            653..1615
                     /locus_tag="IWI57_002751"
     CDS             653..1615
                     /locus_tag="IWI57_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBC2450841.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
BASE COUNT          574 a          262 c          351 g          446 t
ORIGIN      
        1 aatcaaaata tgtgagatag atgatttttc taataaaaga aattataaat gctgatttat
       61 cagttgtttt aaatgagtaa aatcttaaaa aaagctcata tagtaaaaaa atgatctttt
      121 aaggttgcat tttgaaaaaa gttaagatat agtttgaaca tcaaaagatt tgataatcaa
      181 actaattagt ggacggtgga acatggaacc agatttaaaa acagtaaatg attatcttgt
      241 tagtgtcttc aatgatattt tgacaatcga agagtctgag ctgaaaaaat ctcaattcaa
      301 agatctgtca atcacagaga tgcacacgat cgaggcaatc gggatgtaca agaaaaaaac
      361 aacttcagaa gtagccaaag aactatcaat tactgttggg accttgacca ctgctattaa
      421 caagttagta aaaaaaggct atgtcgaacg tatccgcagt gaagatgacc gccgcgttgt
      481 aaagttggga ctaacgaaaa aaggaaagtt actatttcgg gtccatcagc attttcatcg
      541 tgaaatggtc aagaacatct tagacggaat ggctacggag gaacagcatg ccttgctgga
      601 tgcactgaaa aacttgcacg acttcttgca agactataag tgaggatatt taatggaaca
      661 atttggaatg attactgcaa cagccagcgt ccttcctgaa aagattatta ctaataatga
      721 tctttccaag atgatggata cctctgatga atggatttcg tcaaggacgg gtattcgtga
      781 acgaagatgt gtaacaaatc aggagacttc tgatttatgc attttagtgg cggaaaaatt
      841 gataaaacag aaaaatctaa accctaaaga tttggatttt attttagtag caacaatgtc
      901 gcccgattac acgacaccat ctgtagcagc acaagtacaa gggaaattgg gagcaactca
      961 tgctatcgct tttgatatca gtgctgcgtg ctcaggattt gtttatgcgt taagcatggc
     1021 tgaaaaaatg atccgctgcg gctcaactaa gggactggtg attggtggtg aaacactatc
     1081 taagctaatc gattggtctg atcgtacgac tgctgtgttg tttggcgatg gagcaggtgg
     1141 gattctctta gaagcgaatc cacagaaaaa attgctgaaa gaaaaattag cagctgacgg
     1201 taccagaagt agctcattga ccgctggcta ccagcaaaat aaaaatccgt tctattcaga
     1261 agattcagaa ggttcgtatt atttgcaaat gactggcaga gatatttttg attttgccgt
     1321 acgagacgtt gcaaacaata tccaagaagt catgaaagac aaaccagttg attatctgct
     1381 tttgcatcag gcaaacctca gaattattga gaaaattgcc cgaaaagtaa agatgccgca
     1441 agaaaagttc ctgaccaata tggataaata tgggaataca tctgctgcaa gcatccctat
     1501 cttattggat gaagcagttt ctagtggaaa gatcacctta ggaaaacagc agaaagtaat
     1561 atttacaggc tatggcggag gtttaacatg gggaagtatt ctcatggagt tgtagactgt
     1621 taaattataa aaa
//
LOCUS       DADXTG010000239         1626 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.246, whole genome shotgun sequence.
ACCESSION   DADXTG010000239 DADXTG010000000
VERSION     DADXTG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.246"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            129..>1626
                     /locus_tag="IWI57_002752"
     CDS             129..>1626
                     /locus_tag="IWI57_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBC2450842.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFL"
BASE COUNT          632 a          223 c          283 g          488 t
ORIGIN      
        1 tgcaggcttt tttattgatt tgaaataatc aagaatatta gaaacttcta cattaatcaa
       61 gaactttctt ctgtatgaaa atagagataa taattgtaca cttaagctgt attcaggagg
      121 tgaagataat gggcatcgaa tttagcgaac ggcaaatgaa aatactatac tttttgattg
      181 aaaatgagca cacaacttac aaagaattat cagaacaatt atatgtatct acgaaaacaa
      241 ttagcaaaga tattttaatt ttgaaagaaa taatccaaaa aatgccagat gtacaaatgt
      301 tggttcaacc taaaaaaggc gtttcgctta ttggaaaaaa agaaacactt cgaaatttat
      361 tatccagtca taaaatcaat atcgcagact ctccggagga ccgtctaact tatgtgtttg
      421 ctaaattatt gaatactaat gattctctaa agattcaaaa tcttgctgac gaattatacg
      481 tgagcaggtc gacggtagaa agcacaatga aagatgttag aaaaagacta aacaagcatg
      541 ggatgtcctt agtttcagat cgaaatggcg tgcgcatcaa agctgatgaa aaaagtaaaa
      601 gaaggttgat gtcagaagtt gtgaactatt attggggtgg ctttacagca tctaacagta
      661 aaacagatct cttagacttg aatatcaatt tcattggtga agcgaataat ttcttaaata
      721 aggacatatt gaaaaaagta gccgaattgc ttaatcgata catgaataag acacaattga
      781 aatttacaga ttatgaattt caaactttag ctatccacct tgctattgcg atagaaagga
      841 ttgaaacaag caatttctta tcacgtagta ataattctga agaagaaatc tcaaagaata
      901 cacttatttt aatcaaaatg attgaaaaag cattttcaat tattgtgcca atagatgaac
      961 aagcttatgt tgacattcac attacttcga ttgaaaaaaa tgctatgaat cacgacttag
     1021 atcaaacgaa gctttcctat ttagatatgg gtgttgagct acaagagtta gtaagaatta
     1081 aattaaatga attttatcct gatgaagaat tgatcaataa tctagtatta catctcaatg
     1141 cagcagttaa aagactcaaa ttgggagtaa atatctataa cccttatacg gacaaaatca
     1201 aatattcttt taaaaggtca tttatgataa gtgtagattt attagaggaa atcgaagaga
     1261 gattctgtat acattttaat gaagatgaaa tcgcttacgt aacactccat attcaatcac
     1321 ttttggatcg ttacaaacct gacaaaacga aagtgatatt agtttgtagt agcggatatg
     1381 gaacatcaaa attattagag caaagaataa ataacggttt tgcagatatg gttgagatag
     1441 aagatgtact aagcatcaat gaactacaag atagaaaagt cactgatgaa cttgttataa
     1501 gcacgattcc gattgaaaat acaaattttc ctgttattgt tgttagtcct ttgatgttag
     1561 gggcagatgt cagaaaagta aaacagtatc tacagttaaa tgaacaaaca gaagatgctt
     1621 ttctaa
//
LOCUS       DADXTG010000240         1618 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.247, whole genome shotgun sequence.
ACCESSION   DADXTG010000240 DADXTG010000000
VERSION     DADXTG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.247"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..815
                     /locus_tag="IWI57_002753"
     CDS             <1..815
                     /locus_tag="IWI57_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450843.1"
                     /translation="NMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGY
                     NSTNKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALI
                     ACLVSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDA
                     RAVEYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRD
                     KSVAAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            832..>1618
                     /locus_tag="IWI57_002754"
     CDS             832..>1618
                     /locus_tag="IWI57_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450844.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNK"
BASE COUNT          561 a          214 c          317 g          526 t
ORIGIN      
        1 ttaatatggg attcaatata acctttttac agacaggtgc acgctggggt actagaatct
       61 atatagatcc gatatttatc attttatatt tctttataaa tctatatgcg gttatatata
      121 aaagagaggg ctataatagt acaaataaaa gtatatggaa ttgcattttt agtttactga
      181 catttgttat aatctcgcaa ggcagaagtg gtatattata tgtagtaata acctcggtta
      241 ttatgctttt aatttcgctt aaaccatcca agattattag atttggtata ttaggaattg
      301 ccttaattgc atgtctagtg tctgtgaccc aaattagaga ggtcgttata tcttcgtttg
      361 tagagtctca gagcagtgac acaggtaata tcgcatatag aattgctgaa caagattatt
      421 ttgaaaataa attagaaggt catgaactat ttggagttgg tataccaaac aatcatgatg
      481 ccagagctgt agaatattca ggcaaaacaa tagattttga gtctaaaaaa tataatccct
      541 attatcttga ggacttaggc gcttttgaaa ttcgttatag atttggtata attgcatatg
      601 cgttatatat agttgcttta gctataagta ttatagatgc cggatttcga agagacaaat
      661 cggtcgccgc atttgcgggt atggcattca tggtgtttat ggcgcttaat tttaatttgc
      721 tagcgtatat tacagcaaga ccgttcggat ttatgatttc tttaattttg atagaaatat
      781 cggcattcaa tgatgaattc atatatattg aataggtaag ggagagcaca gatgaattta
      841 gatcgattaa agaaaatagc aaatatgatt tcaccaatca taaaagtgat aaatgcgccg
      901 ttagcgtatt ttagtgggaa atcagattat atggagaagg gaatattgtg gcctactgta
      961 acaacctact ccaataacag tagaaaagtg attcaatata caactttacc taaaaacgta
     1021 gacaacatgg ctcaaatgca atgggctggg gaaagttttg aggacatatg tatagttctt
     1081 caaggtccag tattagaaga cggtgatttt acactaaata ccgttaaaat gtataataaa
     1141 tactatccta aagtaaaact tgtagtgtct acttggatag gattatctga agagacagaa
     1201 tgcaagttgc gtgaagctgg tgcagatgtt gtattatgca agaaacctga aaaaacaggt
     1261 gcgtacaata ttaatctaca acttacttct gcatacgaag gcgtaaaaat ggcgcaaaaa
     1321 ctggggtgta gttatgcgct gaaaacgcga actgatcaaa gactatatgc aaatgatgta
     1381 ctaaagtatt tccaaacatt attgaatatt tttccatctt cggactgtaa agtagtgaat
     1441 cagcgattaa tatttacgac ttatggaaaa tcatttagat atcttccgtt taatttatgt
     1501 gattttttgg tatacggaga aactagtgaa ttattaaaat tatatggtat tcaaagagat
     1561 aaccgagcat cagactttgg gaaaattatt tatactgagg aaaaagagtt taataaaa
//
LOCUS       DADXTG010000241         1584 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.248, whole genome shotgun sequence.
ACCESSION   DADXTG010000241 DADXTG010000000
VERSION     DADXTG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.248"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1584
                     /locus_tag="IWI57_002755"
     CDS             <1..>1584
                     /locus_tag="IWI57_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBC2450845.1"
                     /translation="EEKIGQLFVNMGSSRDKNYLSSMVNQYHIGAVRYNPGTADEVYD
                     QNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKIGATNDAKYAYELGRVSGIE
                     ASAIGCNWSFAPIVDINRNWRNPIISSRSFGQSPDKVLELSLAYMKGIQESGILPAAK
                     HFPGDGIDERDQHLSFSINSLSTEEWDNSFGKVYSGLIEAGLPSIMAGHIHMPAYSKH
                     FNLDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGAMKRSELLPTSI
                     AAGCDLFLFFNDPDEDFNYMMEGYYKGIISEERLRDALRRILGLKAKLGLHKKEKNKI
                     LLPKQEAMEKIGLIENKSIFKEVADQAITLVKDKQDIWPIVPEKYKRILLVDVHGVKG
                     GFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKIKNIAGIYAAKR
                     PIKNLTKKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHLA
                     DVPQVKTYINTYDGKQETL"
BASE COUNT          572 a          207 c          317 g          488 t
ORIGIN      
        1 agaagaaaaa ataggtcaat tatttgttaa tatgggatct agtcgtgata aaaattactt
       61 aagtagtatg gtcaatcagt accatattgg agctgtacga tataatccag gaactgctga
      121 tgaggtatat gatcaaaact atatcctaca aacagaaagc aaaattcctt tattaattgc
      181 tgctaataca gaagcaggtg gtaacggagc ttgtacagat ggtaccgaag ttggtgtaga
      241 agtaaaaatt ggtgcaacaa atgatgcgaa atatgcttat gaattaggga gagtttctgg
      301 aattgaagca tcggctattg gatgtaactg gagttttgct cctattgtag atattaatag
      361 aaattggaga aatcctatca tttctagtcg atcttttgga caaagccctg ataaagtttt
      421 agaacttagt ttagcttata tgaaaggtat tcaagagagt ggaattttgc ctgcagctaa
      481 acatttccct ggtgacggta ttgacgaacg agatcaacat ctatctttta gtattaatag
      541 tttaagtaca gaagaatggg ataattcttt tggaaaagta tactcaggct taatagaagc
      601 tggattacct tcaattatgg caggtcatat ccatatgcca gcttattcaa aacattttaa
      661 tcttgatctg aaagatgaag atttacttcc tgcaacttta tcaaaagaat taatcactga
      721 cttacttaga ggaaaactcg ggtttaatgg attagtagtt actgatgcta gtcatatgtt
      781 aggaatgact ggggcaatga aacggtcaga attattgccc acttctattg cggctggatg
      841 tgatctattc ctatttttta atgatccaga tgaagacttt aattatatga tggaaggtta
      901 ttacaaagga attatttcag aagagcgttt acgtgatgca ttaagaagaa ttttaggctt
      961 aaaggctaaa ctaggtttgc ataaaaaaga aaagaataaa attttgttac cgaaacaaga
     1021 agctatggaa aaaattggtt taattgaaaa taaatcaatt tttaaagagg tagctgatca
     1081 agcaattaca ctagttaaag ataaacaaga tatttggcct attgtaccag aaaaatataa
     1141 aaggatttta ctggtagatg tacatggggt aaaaggtggg tttggtgaag tagttgctgg
     1201 aaatagacct caggccattg atttattgaa acagattttg tcagcaaaag gatttgaagt
     1261 tgaaaaatac atttcacctg aagaagaatt gaaaggatta tcgcaagaag aaaaaattaa
     1321 aaatatagct ggaatatatg ctgctaagag accaattaaa aatttaacta aaaaatatga
     1381 cttgatcatt aatatagcta atgttaatcc aggcacagtt caacgaattg tctggccagc
     1441 aagtaaagga actccggata taccgtttta cattcatgaa attccaacaa tttttgtatc
     1501 tgtacagtgt ccgtttcatt tggctgatgt acctcaagta aagacttata ttaatactta
     1561 tgatggaaaa caagaaactt taga
//
LOCUS       DADXTG010000242         1511 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.249, whole genome shotgun sequence.
ACCESSION   DADXTG010000242 DADXTG010000000
VERSION     DADXTG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.249"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..542
                     /locus_tag="IWI57_002756"
     CDS             <1..542
                     /locus_tag="IWI57_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBC2450846.1"
                     /translation="SFLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVL
                     VLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVA
                     MLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFV
                     ISFLISPKQKFLRKKERSL"
     gene            539..1489
                     /locus_tag="IWI57_002757"
     CDS             539..1489
                     /locus_tag="IWI57_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBC2450847.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
BASE COUNT          550 a          193 c          290 g          478 t
ORIGIN      
        1 gttctttttt agcactagga gttatcctta taggagttgc aaatagctca acggatttat
       61 ttcatatttt atttggaaat attttagctg tacaagatat tgataagtgg attacaattg
      121 gcgtttcggt tttagtgttg gttatagtag ttctattttt taaagaatta ttaatcactt
      181 cttttgatcc attaatggca aaagcaatag ggatgaatgt cagtttttat cattatctat
      241 tgatggttct attaacttta gtagcagtaa cagctatgca aagtgtggga actatattaa
      301 ttgttgcaat gttaattaca ccagcagcta cagcatattt atatgcaaat agcttgaaaa
      361 ctatgatatt tatttctgca acagtcggag caagttcatc attgcttgga ttgtttattg
      421 gttatagttt taacatagca gcaggatcaa gtattgtttt aacagcggca ttcttatttg
      481 taattagttt tctaatctca ccaaaacaaa aatttttaag aaagaaggag agatcgttat
      541 gatgagaaaa tggaaagcag tactgggaag tctggggatt ttgattgctc tttttatatt
      601 cggggcatgt tcaataaata gtaaagacaa agataaagtg gcttcgaacg aaaaattaaa
      661 agtagtagtt actaattcga ttttagcaga tattaccgaa aatatagcaa aagataaaat
      721 tgatttacac agtatcgtac ctattgggaa agatccccac gaatatgaac ctttgcctga
      781 agatgttcaa aaaacttcaa aagcagattt gattttttat aacggtgtta acttggaaac
      841 tggaggaaat gcttggttta caaaattagt taaaaatgcg aacaaagagg aaaacaaaga
      901 ctattttgca gcaagtgatg gcatagatgt tatttactta gagggtcaga gtgagaaagg
      961 gaaggaagat cctcatgctt ggttaaattt agaaaacggt attatttacg ctaaaaatat
     1021 tgaaaaacag ttagcggaaa aagatcctga taataaaaaa ttctataaag aaaatctaga
     1081 taagtatatt gaaaaattgg attctctaga caaagaagct aaatctaaat ttgcttcaat
     1141 tccgaatgat aaaaaaatga ttgttacaag tgaaggatgc tttaaatatt tctcgaaagc
     1201 gtataatgtg ccttctgctt acatttggga aatcaacact gaagaagaag gaacaccaga
     1261 tcaaataaaa cacttagttg aaaaattacg cacaacaaaa gttccctcct tattcgtaga
     1321 aagcagtgtg gatgatagac caatgaaaac agtatcaaaa gataccaata ttcctatcta
     1381 ttcaacgatt tttactgatt caattgcaga aaaaggacaa gatggtgata gttactatgc
     1441 gatgatgaaa tggaacctgg ataaaattgc tgaaggcctt tcgaaataat ttggagggta
     1501 ggtatggtac a
//
LOCUS       DADXTG010000243         1491 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.250, whole genome shotgun sequence.
ACCESSION   DADXTG010000243 DADXTG010000000
VERSION     DADXTG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.250"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..1356
                     /locus_tag="IWI57_002758"
     CDS             64..1356
                     /locus_tag="IWI57_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBC2450848.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          463 a          263 c          318 g          447 t
ORIGIN      
        1 ctaggcaggt ggttgcttcg cggtttttct ttttctacaa taagatcaag aggtgattac
       61 acgatggcta gaaagaaaag aaatcctgat gccgaaaagt tagctgaatc cattctgaat
      121 gcctatcaac ctgaatctgt cgatgacatg caagatgctt tgaaagatgt gtttgggccc
      181 ctttttgaaa aaatgcttca aggagaattg aataatcatt taggttatga tgcccattct
      241 aaagagccta aggaacacga taaccgtcga aatggctatg gaactaaaac gcttaaaacc
      301 agttttggtg aagtagctat tgatgttcct agagaccggg aagcttcctt tgaaccagag
      361 ttaattccta agagaaaacg agatgtctcc gacatcgaag ggaaggttct ttccatgtat
      421 gcacgaggaa tgagtcaacg agatattgcc gcaaccgtcg aagctattta tggctttgat
      481 atttcccatg aaatgatttc agatatcact gacgctgtcc ttcctgaatt ggaagaatgg
      541 caagcccgcc ccctagcaaa gtgttatgcc tttctattcg ttgattgtat gtatgttact
      601 ttacgagaaa attatgaagc caaagaatat gctgtataca ccattcttgg ctatgatctc
      661 aaaggaaata aggagatttt aggattatgg ttaaatcaaa cagaatctaa aaatcgctgg
      721 atgcaagtat ttgatgaact gaaagcacgt ggtgtcgaag atgtcttttt catttcgatg
      781 gacggtgttt ctggcctcga agaaggcgca aaagcgatct ttccatcggt aattgttcag
      841 cgctgtattg ttcatcttgt tcgaaatgca ttgcgctata ttccaagtaa ggactataag
      901 gaagtctgcc gagatatgaa aaagttctac ggtgcttctt ctctaaacgc tgcacatgct
      961 gcttttgaca gttttcaaaa tcggtggtct cattattctg gtgctgtaga tgtctggaaa
     1021 cggaactttg cacatgttga acaactattt gattatggta gtgccattcg aaaaattatg
     1081 tacactacca atgcggttga aagtgtccac tccagcttca gaaaagtcac aaaaaaagga
     1141 gcgttctcta atgagaatgc actcctaaaa ctactttatt tacgcacgaa agaattacac
     1201 gcgaagtggt caggtggtcg aattcaaaac tgggctatgg tgcttaatca gttaatgatc
     1261 aacgagacct tctcctctag aatgaaaagt atgcgattta ccttccgtaa caacggtagg
     1321 tttattcaac tatgtgtccg atcaaaattt atctgatttc aaaatagccg agagaattat
     1381 ggtggttaaa aactcttaac tatttaatta cggttttcat agtttttgga ttcaggagga
     1441 ttaaaacgaa ttctataaaa aaagatttgg tttaaatgaa tgattaaacg a
//
LOCUS       DADXTG010000244         1464 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.251, whole genome shotgun sequence.
ACCESSION   DADXTG010000244 DADXTG010000000
VERSION     DADXTG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.251"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            39..1151
                     /locus_tag="IWI57_002759"
     CDS             39..1151
                     /locus_tag="IWI57_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBC2450849.1"
                     /translation="MLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQ
                     QLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKA
                     LTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTD
                     EEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHT
                     KFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQV
                     LSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAI
                     IMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          516 a          266 c          235 g          447 t
ORIGIN      
        1 ctaaaaaaag aaaatattca tttttaatga tcgttgtcgt gttgtttagc cttagtgcgt
       61 tctctacagg aactattctt ggatggcaat tttacaataa tgaacactct cagatagttg
      121 cccatgctca atcagctcaa aaaaagttga tactgactac caatgaacaa cagctcgttg
      181 aaaaaatcga tcaactatta agaaaaaatg attatattgg ctcaatttac gtaaagaaaa
      241 ataatcgcat catcatggaa aaaggctatg gctatgcgaa taaactcacg caacaaccaa
      301 atgatccttc tctttattat cagattggtt cggttcaaaa agcattgact actcttttga
      361 ttttaaaaca agtcgagcgt gggcaccttt ctttagatac taagctggct caattctatc
      421 cacaaattgc tggcagccag caaattacta tccgtgactt gctgtatatg cgctcaggct
      481 tgcaccgaac tgcttctcca accataccga tgactgatga agaagtcgtt caatttgcga
      541 ttcagcactt aaaatttaca gattatcaga cctctcatta tgattcattg aactttacca
      601 tattgactgg aattttgatc aaattaacaa atcaaccgta tgaaaaatta ttgaccgatg
      661 atatcatcga acctttagga ttacagcata caaaatttta tgaccaagtc aaaaattcat
      721 ccaatcatgc actatcctat caaatgtcta aagaggatga ttactttaaa ccactgacag
      781 aatctgaaac agagatccat aacgaattag ggactggtaa tatcagtatg tccgtctatg
      841 atttaaatac gctgtttaca caagttttat ctggaaaact agtttccaaa gacttactct
      901 tctcattatg gcgtgaaaat gcgaatggac atccatatag tggtggggta tatagcggag
      961 ataattatat cctgtcgcaa ggaaatatcg actcttttga tacatctgct atatttagca
     1021 aagattcaca ggatgctatc atcatggaaa gcaatataaa agctgacaaa actatcaaac
     1081 tgcctactgc tgatctaaga aaccaaatct atgacctgat ggaaggaact actaagttgg
     1141 caaatagctg atatgatgaa aagatagcaa atactacttc tttgaatagc atttgcttaa
     1201 aaaaagtact cgactaaacg taggaaagag tgtgggacaa atctaaaaat caatttttgc
     1261 tacagacttt aattccaaat aaacagaaca gaaagaggtg ttcttctttc tgttctgtcc
     1321 aaattattct aagatttctc tttaccaaca ttatttgaca aagggcctat taaatctaaa
     1381 atagtatctt tagattgcta aaatatgtgc tagcaaatca ttcatggctt gttcttattt
     1441 ttaattttat gcaataaaaa agaa
//
LOCUS       DADXTG010000245         1449 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.252, whole genome shotgun sequence.
ACCESSION   DADXTG010000245 DADXTG010000000
VERSION     DADXTG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.252"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            439..1251
                     /locus_tag="IWI57_002760"
     CDS             439..1251
                     /locus_tag="IWI57_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450850.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          477 a          221 c          248 g          503 t
ORIGIN      
        1 ttattctttt ttgtttttta gggatgtaca aaagatctca accacaggag acattgcttt
       61 tcacttttct agaataaaat gcttgtcctc agtattttct ggtccaataa attttaccac
      121 atttaatagc tatggcggag gcgtgaattt ttttatccat atctaacatc tttccagccg
      181 taatatttta ctggacaaca aacaatttga ttctaagtta tatacttact atacgttggg
      241 atactgaata tctgtacaat tctagtgagc tatcaatatg gacaaaagaa tagatgcagc
      301 cttcttattc agctgtttgt atagctttta tggattgata tatattagcg tactgcaatt
      361 tcagaatcaa ttgcgttgac gattgtacca gttgtcttgt attacacata tactcttctt
      421 tatgaaagaa aaaactgcgt gattctttta gctgtcagta tgagtttgct catctatacc
      481 catattctct ccacctttat gagtgtgatt gttattggct tcttgtttat cggctctctt
      541 ttctctaagc ggaaagagag aaaagaaatg tcgaatctga tatcaaatat gataaaagct
      601 gttggttgga ccattctttt aacagcttac gtttggtatc cgatgttaca acaagtccta
      661 catcaaaaaa ttaatcgtcc ttttcgcaca aaacttcaag aaagggcatt aaacatttct
      721 gatagtttaa tcggtgcagt aaataatgat ttagttacat ttacaatggg aacaattggt
      781 gtggtttcat ttattcttcc acttttcttt aaaaaaaaat ttgccaataa agaaaagatt
      841 attaattttt gtgcagtgac cacttggttc ttatcaacga atctttttcc atgggaattt
      901 ttacaaaaaa cacctattca aatcattcag tttccttgga gaatcttggg ttttcaagtg
      961 ctttttggtt cattgatctt aattatagtg tttttaaagt ggaaaacaag caacaaaaaa
     1021 agtatgtgta gccttgtagg aatagtcctt cttattttta cagtgactgt agcaacagaa
     1081 gcgaattatt cgcagaaaat tcaatcgtat aaaggtcgtt taattatgac caaaaaagat
     1141 gttacacttt atacaacctc gagaactgga ggtctttatg actatgcacc attagatgca
     1201 ttgaaatata aagatcattt aaagaaacat gaagtaaaag tctatgattg atgggagaat
     1261 gcatctcata aagcacataa ttctaaaata atctatacag taggagatgc gaatgagcaa
     1321 aatatcacgt tacctgtatt taattactgg gaacagttat gaaagtaaat gggaaaaaga
     1381 gcactataga aaatgacaat ggtttagtgt ctattaaagg tacgggaaaa agaactatag
     1441 ttgaggttt
//
LOCUS       DADXTG010000246         1439 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.253, whole genome shotgun sequence.
ACCESSION   DADXTG010000246 DADXTG010000000
VERSION     DADXTG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.253"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            530..862
                     /locus_tag="IWI57_002761"
     CDS             530..862
                     /locus_tag="IWI57_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBC2450851.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT          484 a          212 c          302 g          441 t
ORIGIN      
        1 attacttctt ttcaggcaat acgaatatta ttcgcttaca ttttttgtat aaatttaatt
       61 gtctgctatg cttgtgatga attgattttc gttctgcact tttggatgat aaatttggac
      121 aattgaaaat acaaaaaaaa gaaaggcggt atgaactcgg aaggctggta accttcgtca
      181 gcgttcatac acggcattaa gatttggccc ttaatgcgtt aacctttcag tgttacggta
      241 ttacccgcaa tggatttagt ataacagata ttcaaaagtg ataccacttt ttttggctgt
      301 gttaaggtta aaaatacgtg atattacgat gaatctatgc ctaagcacaa tgttataaaa
      361 atgtccgtgt ggctctttgc cgtatgacga acgctgaaat tagagggaac gcatgtttgg
      421 atgcgttccc tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata
      481 acaagaaaaa cttgttaaaa atatttaaag aaattgggtg gtttatttta tgtcatatac
      541 aggaattttg agtctcaaag atatttgtca ctacggtaaa cggtgtaccg ctacagagaa
      601 aatcacgaag aaactatcaa ctggccaaag taaaaccgtt gtgcagtgta agaaatatat
      661 aatccagaaa gacaaagtct cagaggaaat gatttattac attgggaaac aaaagcaaat
      721 aattttaaaa gatcctatcc ccttaaaaga gctatatcca acaatcaaac atgtttatga
      781 ccaaaacggt gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg
      841 gatggggcgt ttgatcggct gaattgatca ttctcataac ttgtatttta tcgtttttcg
      901 cttgcttgaa aatattgtcg agacttctaa aggaacttag agtcactaga aaaataagga
      961 gtagacttga tctatcaaca attcccttcg gggagtgacg gggtaggttt taacgattgg
     1021 taacgtaaaa aattggtcaa tcgagtaaat gcgcttgttt ggatagtggt gaaatgaatt
     1081 tgtgtaaata ataagcgcag agtaaacaag agggaattta gaagttagac aagttagaat
     1141 agcgtctaga ttaatcatga gcagctacta agggatctag actttgacgc aaactcatgg
     1201 aataaggagt aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt cgtgtgctgc
     1261 agtacatgta gatacttgcg aggacgttga ttattggatc aatcgagcat ttaccgctgt
     1321 ctactagggt ttctcgtttc ggacgccata aacgagtcga aattttttat gtatttttaa
     1381 gtaaacgaaa aacgataaaa tatgaacaag tcctgaataa agtgaaaaaa ttgatataa
//
LOCUS       DADXTG010000247         1433 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.254, whole genome shotgun sequence.
ACCESSION   DADXTG010000247 DADXTG010000000
VERSION     DADXTG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.254"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..938
                     /locus_tag="IWI57_002762"
     CDS             <1..938
                     /locus_tag="IWI57_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBC2450852.1"
                     /translation="AIQNFGSKKNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIE
                     ETEKIKIYQLVTKNEKKLFLNTTFENLTLINPNIMNFEDESIIEASGFRIQPEYELFK
                     RLFDIIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTA
                     TAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVD
                     QFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQ
                     TIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            954..>1433
                     /locus_tag="IWI57_002763"
     CDS             954..>1433
                     /locus_tag="IWI57_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2450853.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     "
BASE COUNT          520 a          243 c          251 g          419 t
ORIGIN      
        1 cagcaatcca aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc
       61 ttgataacta ttatgcaaac atcaaaaata tgattaaaga catcgatatt gtctatcttg
      121 cttctcatat cgaagaaact gaaaaaatca aaatctatca attagttact aaaaatgaaa
      181 aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca aatattatga
      241 atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct gaatatgaac
      301 tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt gcttcaccat
      361 ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc atctataaac
      421 aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc tcgatgaccg
      481 ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca cgagtaacgc
      541 ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc
      601 tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt
      661 tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt atcactgggt
      721 atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt tttgatttac
      781 tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa accatcaaga
      841 tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg
      901 cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca
      961 atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt ggtcgaacga
     1021 ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta aatgatggat
     1081 caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa cgttttgaat
     1141 tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata agacagtcaa
     1201 aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag
     1261 attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt tcgtttgttt
     1321 ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc
     1381 actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat gaa
//
LOCUS       DADXTG010000248         1428 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.255, whole genome shotgun sequence.
ACCESSION   DADXTG010000248 DADXTG010000000
VERSION     DADXTG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.255"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..242
                     /locus_tag="IWI57_002764"
     CDS             <1..242
                     /locus_tag="IWI57_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBC2450854.1"
                     /translation="NGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPEV
                     KKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            253..>1428
                     /locus_tag="IWI57_002765"
     CDS             253..>1428
                     /locus_tag="IWI57_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBC2450855.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     "
BASE COUNT          504 a          274 c          301 g          349 t
ORIGIN      
        1 cgaatggtac gtacgatttt gatcgttttt atcaaggaaa aggggatatt aatgctcaga
       61 ttcttttctc ttataaagca gaacaatact acaaaggcta taaaaagcat gaacatgaat
      121 ttgtacctga agtgaaaaag gattggaacg atcaattgaa gcatgagatc cagcaacaag
      181 aaattagaaa gcaaaaacgt gcgatgttgt ttcaacaagg cagacaacaa gaaagggagt
      241 gagatcgtca cgatgaaaac tgtcatttta gcagaaaaac cgtcacaagc gaaggcttat
      301 gccgatagct tttctaaagc cacccgaaaa gacgggtatt ttgagatcca agatcgattg
      361 tttcctggtg aaactgtgat tacgtatggt tttggtcatt tagtcgagtt agattcgccc
      421 gatatgtacg atgaaaattg gaaacaatgg tcattagaac atttacctat tttccctaca
      481 cattatcact atcatgttcc taaagataag aaaaagcaat tcaacgtggt gaaacagcaa
      541 cttcaatcgg ccgacaccat tattattgcg acagatagcg atcgcgaagg ggaactgatc
      601 gcttggacga tcattcaaca agcaggcgcc gatcatggaa agatatttaa aagactttgg
      661 atcaactcct tagaaaaaga agccatttat cagggattcc agcagttacg agatgcagaa
      721 gagacctacc caaaatttga agaagcacaa gcacgccaga tcgccgattg gttgatcggc
      781 atgaacggta gcctactgta tagtttactc ttgcagcaaa aaggcatacc aggaagtttt
      841 tccttaggca gagtccaaac cccaacgctt tacatgattt atcaattaca ggaaaagatt
      901 agaaacttcc aaaaagaacc atattttgaa ggaaaatccc aagtaatcgc tcaaaatggc
      961 gcatttgatg cgaagcttga tccgaatgaa acccaagcga cgcaagaagc atttgaagat
     1021 tatttaaaag aaaagggggt tcaattggga aaacagccag gtacgatcca ccaagttgaa
     1081 accgagaaaa aaagtgcggc aagtccacgt cttttttctc tatccagcct tcaatctaaa
     1141 atgaatcagc tcatgaaagc tagtgccaaa gatactttgg aagccatgca agggttgtat
     1201 gaaggaaaat atctaagcta tccgcgaacg gatacccctt atattacgga aggggaatat
     1261 gcgtatctgt tggatcactt agacgaatac aagcattttt taaaagccga agcgattccg
     1321 actcctattc atacaccgaa tagtcggtat gtgaataata agaaagtcca ggaacattac
     1381 gcgatcattc ctacaaaaac ggttatgaca gcagcggctt ttgaacaa
//
LOCUS       DADXTG010000249         1428 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.256, whole genome shotgun sequence.
ACCESSION   DADXTG010000249 DADXTG010000000
VERSION     DADXTG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.256"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..401
                     /gene="spx"
                     /locus_tag="IWI57_002766"
     CDS             <1..401
                     /gene="spx"
                     /locus_tag="IWI57_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBC2450856.1"
                     /translation="YTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAA
                     TEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFN
                     EDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            415..771
                     /locus_tag="IWI57_002767"
     CDS             415..771
                     /locus_tag="IWI57_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBC2450857.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          522 a          218 c          263 g          425 t
ORIGIN      
        1 tatatacttc gcctagctgt acttcctgta gaaaagcaag agcatggttg caagcgaatc
       61 aacttgaatt tgaagagaag aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa
      121 agatcttggc agcgactgaa ggaggagttc aagatattat ttccactcgg tcgaaaattt
      181 acgaaaagct gggcatagat tttaacgaat taactttaaa gcaaatgatc gtcttattca
      241 aagaatatcc ttcattattg agacgaccta ttttgattga tgaaaaaaga atactaatcg
      301 gatttaatga agatgagatc cggtcatttg tccctcgtga aattcgatgt gttgccagaa
      361 atctgatcta tttacaaaac aataacatct taaattcata gggagggagc cttcgtggaa
      421 gtgaaagtct atggatcagc gcactgtgta acaagtctga aggcgaaaca atggttagaa
      481 caccatcaat tgccttatca atttgttgac ttagaagaac gcgatctaag tacagaagaa
      541 gcagaagaat tatgcagttt tgaagaagtg caagcagaac agctatttgc gacttggtct
      601 gaggcttttc acaacattgc gattgacctt tcctgtgtag gaaaaaaaca actgattttt
      661 ttatgcacac aacataccaa tctcctcaga cgtcctttga ttattatcga tgaagttcta
      721 tttattggat acaatgagca gttgatggaa cagcttatct taaataaata gactttaact
      781 gttttaatca agtcactgag catttcatac agttgtaaca gtcgaacact tagcatcaag
      841 cgtgttatgt gttctttttt tgactttatt tttctactat agagacatgc tcaatttatg
      901 tggtcaaaat tcaaatctag gtggggatat agagttaaaa atatattgac atgtgtaaat
      961 ttacatgata tattcatttc tgttgtttta agtagcaaag caataaaata gtattttaaa
     1021 aaacatataa aatagttgtt gacatgttag aaatgacatg ataagataac tgagttgtta
     1081 aaaaagaacc acttgtgaag cttgattcta tcgaaaaaaa cttcaaaaaa agttcttgac
     1141 aaataacaaa tgataagtta tgataaatga gttgctgtaa gatttcgaaa aacgaaatca
     1201 aaaaaacttt taaaaaagag ttgacaatta cttgtctctc tgatatgata taaaagttgc
     1261 tacggcagcc aaaaacgaat cggggaacac tcctgatgaa gtagaccttt gaaaactgaa
     1321 caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca acaaacattt
     1381 tttaacaagc aagcaatatg ctagcaaaca aattgagctt aacaatcg
//
LOCUS       DADXTG010000250         1378 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.257, whole genome shotgun sequence.
ACCESSION   DADXTG010000250 DADXTG010000000
VERSION     DADXTG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.257"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..400
                     /locus_tag="IWI57_002768"
     CDS             <1..400
                     /locus_tag="IWI57_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBC2450858.1"
                     /translation="YEFEGGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALL
                     LKGTTSVIFSIYSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNM
                     PAPLFLSAVKVGSFVEVKMPGLMAALKRVY"
     gene            419..>1378
                     /locus_tag="IWI57_002769"
     CDS             419..>1378
                     /locus_tag="IWI57_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBC2450859.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDET"
BASE COUNT          506 a          172 c          271 g          429 t
ORIGIN      
        1 ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca agttcgaaac
       61 atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata aagacggtat
      121 ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata gtacctctgc
      181 aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc cagtgatccg
      241 agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa agttgcttta
      301 caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg ttgaagtgaa
      361 aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg aaaatataat
      421 gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt ctttcatgat
      481 gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga tttatgaaga
      541 ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc gtatttttgt
      601 tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata agttactaaa
      661 aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa ttaatttatg
      721 gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa ctgaagatgg
      781 aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat tgttgttaca
      841 aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt ttcaaaacca
      901 tccctttgag atagttcata atggtattga tttagagctt tatagtaaaa attcagaagc
      961 taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg tgggaaacat
     1021 tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt tagaaatact
     1081 aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta gaaataaact
     1141 gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag gagaaagaaa
     1201 cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc tttatgaagg
     1261 tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct tagcagatac
     1321 tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag atgaaaca
//
LOCUS       DADXTG010000251         1373 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.258, whole genome shotgun sequence.
ACCESSION   DADXTG010000251 DADXTG010000000
VERSION     DADXTG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.258"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWI57_002770"
     CDS             191..445
                     /locus_tag="IWI57_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBC2450860.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWI57_002771"
     CDS             446..763
                     /locus_tag="IWI57_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBC2450861.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DADXTG010000252         1366 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.259, whole genome shotgun sequence.
ACCESSION   DADXTG010000252 DADXTG010000000
VERSION     DADXTG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.259"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IWI57_002772"
     CDS             <1..1350
                     /locus_tag="IWI57_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBC2450862.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          549 a          216 c          297 g          304 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaa
//
LOCUS       DADXTG010000253         1364 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.260, whole genome shotgun sequence.
ACCESSION   DADXTG010000253 DADXTG010000000
VERSION     DADXTG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.260"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /locus_tag="IWI57_002773"
     CDS             complement(140..1288)
                     /locus_tag="IWI57_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2450863.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DADXTG010000254         1309 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.261, whole genome shotgun sequence.
ACCESSION   DADXTG010000254 DADXTG010000000
VERSION     DADXTG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.261"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(314..1309)
                     /locus_tag="IWI57_002774"
     CDS             complement(314..1309)
                     /locus_tag="IWI57_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBC2450864.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
BASE COUNT          390 a          230 c          187 g          502 t
ORIGIN      
        1 aggatcaaaa acttttaagt ttctgaccct tttgagttct aattatttct tccagaaaga
       61 agaatgttat ctgctttatc tgcaattgat tctgttagaa ggactgaaaa gtttatgcca
      121 gcccttttgc ctagcttatc cgcccattga ggaattgtta aggtttttct gacaagttct
      181 atgtctttca aatatttggc aacatctaca cagattaaaa gcatgatttt gtatcatagt
      241 ctattataag agatttaaca actttttata tcacgaatta ataatgaaat tatcattgat
      301 agtctttata aatttaattg tcctttttct ttactgatgt cttccccatc cataatgtga
      361 caggtaaaat atattccttt tttgtttctt ctttatttat tcgtttcatc aaaatagcaa
      421 cagctgtgtc tgctatatca ttaattggtt gtattactgt tgtcagatct ggtaatatac
      481 tccgagtcat taaagtacca tcatatccga ctactaataa atcagctggt acatttaacc
      541 ccttttcttt tgctacttgt aatattaggg ccgcatcgat gtcatttgaa gcaaaaatac
      601 catctatttt aggataatcc tcaaacattt ggaaaaaaat ctgttttttc tttacataag
      661 aaatattgaa atctaatgtc acagttcgtg gaattaactg atatgctttc atagtatctt
      721 cataggctag tctccgacga ttagccggag tctgtaagtc aattggaccg tttgtatgaa
      781 taatattttt tgcaccttgt gcaattaata acttggttgc aagttttcca ccattgtagt
      841 tatcggattc aacaactgga atatcttcat tcatcactcg atcaattgca acaatcggta
      901 aattctgata tttatattcc tgaatccctt ggttatgtgt accaacaatt aaaccatcta
      961 cttgatcaga caataactga tttaaataat ttgtttcctt ttctttatta ttcatcgaat
     1021 tgcctattaa taccttataa ccaatttcat ataacttttt ttccaatgcc tcaacgagtt
     1081 cactaaaaaa aggattggct actgtaggaa atagtaaccc gacaatgtta gttttctttt
     1141 gaaataattg acgcgctaca acgttcggtt gataattgag ttcttctata gccttataaa
     1201 cattatcaat cgttttttga cttaaatagc cacgattgtt taatacacgt gaaacagttg
     1261 tttttgatac atttgctctt ttggcaacat cttctaattt tgggcgcat
//
LOCUS       DADXTG010000255         1289 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.262, whole genome shotgun sequence.
ACCESSION   DADXTG010000255 DADXTG010000000
VERSION     DADXTG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.262"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(625..1143)
                     /locus_tag="IWI57_002775"
     CDS             complement(625..1143)
                     /locus_tag="IWI57_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBC2450865.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          384 a          262 c          174 g          469 t
ORIGIN      
        1 tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt atagaattta
       61 tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc ctttgatggt
      121 tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa caaaggctat
      181 ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc ttcctaaccc
      241 aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg ttgagattcc
      301 atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa
      361 atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca taatctcatt
      421 ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa tctaacgatg
      481 caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa ccggcagtta
      541 gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc tcctttccac
      601 aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc ttatctttgt
      661 tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata cttcccatac
      721 agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata gatcttcatc
      781 actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa tttcctctaa
      841 ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact ctcctaactg
      901 attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt catcattttc
      961 ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag caggtgttct
     1021 atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa attctaaaat
     1081 atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt tatatgttct
     1141 cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata atttcatcca
     1201 cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg
     1261 tgaatgattt ataaaatatc ccaacgtct
//
LOCUS       DADXTG010000256         1282 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.263, whole genome shotgun sequence.
ACCESSION   DADXTG010000256 DADXTG010000000
VERSION     DADXTG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.263"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(128..535)
                     /locus_tag="IWI57_002776"
     CDS             complement(128..535)
                     /locus_tag="IWI57_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450866.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(554..>1282)
                     /locus_tag="IWI57_002777"
     CDS             complement(554..>1282)
                     /locus_tag="IWI57_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBC2450867.1"
                     /translation="NAMIRNRKTLIIDLDPQANLTQVIARTFGITDIKTTITNGVANG
                     ELASSIIKVNENLDLLACDTSFRSFSAFANKHENELDQVTVLKKLLLPIKNNYQEIFI
                     DVPPTISEFSDNAMAASDYSIISFQTAEESLEGVNKYVNYQKFMVDRYDLDLQIIDIV
                     PCMVEPNDDFDEEILEEAIEKYGTVVSKNLIHYQKRLRRYSKIGIHLRKYKNGNFDQW
                     DYKAHQVFINILAELDARQEFLNK"
BASE COUNT          367 a          207 c          178 g          530 t
ORIGIN      
        1 ttgatttaat cgcaaattta cacgttgttt attaagttat accttaaata tttaatgtat
       61 aacttatact ttaatattca tttatctagt ataacttata ctttaattga ataccgcatg
      121 aaaaaaatta ttttggtaat ttttcaaact cttcttgtat ttcttctctt atgtgttctt
      181 cttttgctac tcctagtcta tgctggatat atgagtctgc taatgtatta acaatttttt
      241 cgaaagaaat tctccctttt tcactttgta actccgtcat atagtcttgt aaaattgaga
      301 gtttgctatt taatgccgga gagatctttt taggaactga taccttgttt cttttatcag
      361 gaattgaaac tggtcggcct ctttttttca atgtaatatt tcttgtgtta ctcaatgcac
      421 tattgctaaa gttatattca ccgcttattt tcacatcttg gcgtttgtta ttgggtaata
      481 atggctcaaa ttcaccattc tccatcattt taggaatatc aaaatcttta ggcattactt
      541 atgcaactcc ttattattta tttaaaaact cttgtctagc atctagttct gccaaaatat
      601 ttataaatac ttggtgtgct ttataatccc attgatcaaa atttccattt ttgtattttc
      661 ttaaatgtat accaatttta gaataccttc tcaatctttt ttgataatga atgagatttt
      721 tggataccac ggttccgtat ttttcaattg cttcttctag gatttcttca tcaaaatcat
      781 catttggttc taccatacaa ggtacaatat ctataatttg caaatcaaga tcgtatctat
      841 cgaccatgaa tttttgataa tttacatatt tgttgactcc ctctaaggat tcttcagctg
      901 tttgaaatga aattatcgaa tagtctgaag ctgccatagc attatcggaa aattcgctaa
      961 tggttggtgg tacatcaata aatatttctt gataattgtt ctttattgga agaagtaatt
     1021 ttttcaagac agtaacttgg tctaattcat tttcatgttt attagcaaaa gcggagaatg
     1081 atcgaaaact agtgtcacat gccagaaggt caagattttc atttacctta ataatactac
     1141 ttgctaattc tccatttgcg acaccatttg ttatagtggt tttgatatcg gttataccga
     1201 aagttcttgc tatgacttgt gttaagttcg cttgagggtc taaatcaata ataagcgttt
     1261 ttctatttct tatcattgca tt
//
LOCUS       DADXTG010000257         1252 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.265, whole genome shotgun sequence.
ACCESSION   DADXTG010000257 DADXTG010000000
VERSION     DADXTG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.265"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..214
                     /locus_tag="IWI57_002778"
     CDS             <1..214
                     /locus_tag="IWI57_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBC2450868.1"
                     /translation="MNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFK
                     WLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            219..1166
                     /locus_tag="IWI57_002779"
     CDS             219..1166
                     /locus_tag="IWI57_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBC2450869.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          410 a          179 c          261 g          402 t
ORIGIN      
        1 aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt
       61 tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga
      121 agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct
      181 tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg
      241 gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa
      301 tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca
      361 cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata
      421 aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac
      481 cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca
      541 agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg
      601 aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg
      661 ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta
      721 gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg
      781 gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat
      841 ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc
      901 aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa
      961 cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag
     1021 aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata
     1081 ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta
     1141 attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag
     1201 gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata ga
//
LOCUS       DADXTG010000258         1240 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.266, whole genome shotgun sequence.
ACCESSION   DADXTG010000258 DADXTG010000000
VERSION     DADXTG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.266"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(89..433)
                     /locus_tag="IWI57_002780"
     CDS             complement(89..433)
                     /locus_tag="IWI57_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBC2450870.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT          342 a          289 c          168 g          441 t
ORIGIN      
        1 acgtttaatt aatccttgtg tataagatga atactcttca atcttctctg tatcatactc
       61 tctgccattg tcattgatga tcatgcactt accacctttc acttgcatcg aagttagcaa
      121 agctttctat cttcttctct tgtttatcta atgctgtaag atatctgcca tgaacttcat
      181 tatgatgttc aacacataaa caaataagat tatctaaatc taaagctaag tcaggtctat
      241 ccttgacttc ctttatatga tgaacgttct ctactctatg atacttacct agtcttctac
      301 actcttggca ttcatagtga tctcgtttca tcgctttctc tctaagcctg cgccatttag
      361 gagactgata gaacttaacc aaacgatctt ctcttatcaa ctgtaataac catctataga
      421 attcctcggt catgtcccat ctcctttcgc aatcttattt aatacttagc tattcttttg
      481 ccatacaatg gaataacttc attgctttcc ttccgtttat atgtatcgct ctttattggt
      541 cttctatact ttcgtactat ctcaccgtta ccgttttgca cagtgattac ttcatgcttc
      601 tgttctaagt attgtggtct atacattgtt gttacctcct tttttcaaaa taaaaagacc
      661 actcaaagag tgatctaata tgtaatgcac aggcagggac gtttccgatc ctgtgcttga
      721 gtcatttgac gattcatttg taccaaatcc caaaactcaa tctaacctaa ccaactatgt
      781 aatagcaacc tacaccgatt ccatcgatta ctatcgacct cgccttgctc gtgtactttg
      841 agcgcccatt tccaaccctc ggttgctagc ggttttactt tcgggatctt gtaccgcaaa
      901 cccaaagtca ctggagtgga atcgcaccac acacgagaac ttactaggct ctcacgaggc
      961 tactcgtcat ttaccgttgt gtcttctatt tccgccacag tgacactcga cactataaaa
     1021 ttattcttgg ctgctactat tttttatttt gcccattttt aaatccaatc atatagacat
     1081 taagacagag cgcaaaaatt gaaattatta atggaatcat ttctcttcac ccacctttag
     1141 ttatcgtgtg aataattaaa aaacaataga cagcaacaaa ataacattgc tttgataatt
     1201 tggtataaac cactataaat ttcttttctt gcaattattt
//
LOCUS       DADXTG010000259         1226 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.267, whole genome shotgun sequence.
ACCESSION   DADXTG010000259 DADXTG010000000
VERSION     DADXTG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.267"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..511
                     /locus_tag="IWI57_002781"
     CDS             <1..511
                     /locus_tag="IWI57_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBC2450871.1"
                     /translation="VSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFL
                     VLTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGV
                     GGWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLEN
                     YNLLNQKHN"
     gene            522..>1226
                     /locus_tag="IWI57_002782"
     CDS             522..>1226
                     /locus_tag="IWI57_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBC2450872.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRT"
BASE COUNT          434 a          141 c          242 g          409 t
ORIGIN      
        1 tgtttcacct gtaagtttaa ttttcagtaa aaaatacggt aaaaaagaat tggcgtcagt
       61 agggatgttc ttctctgcta ttgtatattt acttttattt ttgataaagc ctagtaatat
      121 gtatgtattc ttagttctta cttctgttgg atatatgggg ttaggtattt ttaatacagt
      181 catttgggcg aatattactg atgtaatcga tgatcaagaa gtgaaatctg gccaaagaga
      241 agatggaact atttatgcag tatattcttt tgcccgaaaa attggacaag ctttagctgg
      301 cggtgtcgga ggttggactc tttctataat agggtatgat tcgttagcaa aagtacaaag
      361 tggagaagtt ttacaaaagc tgtataactc ttcaacgttg attccagcag tttgtttctt
      421 tgttgtagga ttgattttat ttttcgtata tccattatct aaagaaaaag tattagaaaa
      481 ttataatttg ctaaatcaaa aacataacta ggagaaaaaa catgaacagt tggatatttt
      541 gtattgattc agatggttgt gtcatggata caatgactta taaacacaat ctctttttcg
      601 ggccaattgc tgcagagatt tttgatgttc atcataggga aaaattccta caggattgga
      661 ataatattaa tttatattca gagactcgtg gaataaacag atttttaggt ttagttaaaa
      721 cattagaaaa aaatgatgta ccatcgatta atcgattaaa gcactgggta tctgtaacaa
      781 aagaattgtc taatccatct ttgaagaggg aaatagaata ctctcagtca gaggatttat
      841 taaaagcttt aaaatggtcg gaagaagtca atcgacagat tagtcaagct aaaggattag
      901 ataaaccctt tttaagtgct agagatacta taaaagctct gaaaaaatat gggaagataa
      961 ttattgtaag ctcagctaat aaggaagcta ttcaagaaga atgggaaaga catgaattat
     1021 tatccttagt tgatgaactg tgttgtcaag ataaaggaaa aaaagaagac attattcgaa
     1081 gtgtaattga aaatggttgt gatttggata agatattaat gattggtgat tctcctggtg
     1141 atctagaagc tgcaaataag aataaagttt tcttttatcc tattttggtt aataaagaaa
     1201 aagaatcatg ggaaaactta agaact
//
LOCUS       DADXTG010000260         1217 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.268, whole genome shotgun sequence.
ACCESSION   DADXTG010000260 DADXTG010000000
VERSION     DADXTG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.268"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..412
                     /locus_tag="IWI57_002783"
     CDS             <1..412
                     /locus_tag="IWI57_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBC2450873.1"
                     /translation="MNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLA
                     QYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMI
                     YVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(698..>1217)
                     /locus_tag="IWI57_002784"
     CDS             complement(698..>1217)
                     /locus_tag="IWI57_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2450874.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          393 a          210 c          224 g          390 t
ORIGIN      
        1 gatgaatgct agaaagaaag aaatagcagt attactatca ttaggtatat caaaattaga
       61 aatttttggt caattcctaa ttgagatggt atttatatca atccccgcat tcgttggttc
      121 ctatttctta gctcaatata cagctgacaa gcttgggaat aatatattga ataaggttac
      181 tggtgatata gctaaacaaa tagctaaaca atctgcatct agccaattag gtggtggggc
      241 agaagctgaa ggatttaaca agacattatc aagtttagat atcaatgtat tacctaaatt
      301 catgatttat gtagttatat ttatgagttt agtacttctt gtatcattga ttatttcttc
      361 attcaatata ttaagaagga atccaaaaga attattaatt gacaataatt aaaattttcg
      421 gtgcttttta gcaccaaaaa tgagagtgta atataaaccg tgtaagtaac catcaatcct
      481 aggcgggtgg ttgcttcacg gtttttattt ttctaaaata agatcaagag gtgattacac
      541 gatggctaga aagaaaagaa atcctgatgc tgagaagtta gctgaatcca ttttgaatac
      601 ttatcaaccc gaatctgtag aggatatgca agatgtttta aaagatgtgt ttggtccctt
      661 atttgaaaaa atgcttcaag gagaattgaa taaccattta gggtatgacg cccattctaa
      721 agagccaaag aaacatgaca atcgacgaaa cggttatggc aataagacgc ttaaaacgag
      781 ctttggtaaa gtaaacattg atgttcccag agaccgggag gcttcttttg aacctgagtt
      841 gattcctaat agaaaacgag atgtctccga catcgcagac tgtagacaaa agctgttcca
      901 aactcgtaac tcgagcttgg aacagctttt tatctacttc gtttcgaatt tttgaacaaa
      961 aaaataaagg ctccttgcct ttccatagcc aggtggctaa tttctttaaa ttcattgcag
     1021 caaacactag catcgtatgc gtcgcgactt tttccaagcc tcgatacttg gtccaacgca
     1081 tcccatgctt ttcttttgca tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt
     1141 aaattgattt attcagatct gttaggcgaa gatgttccac ttcatccatc agatctcccc
     1201 aaacatgtct cgtgatg
//
LOCUS       DADXTG010000261         1213 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.269, whole genome shotgun sequence.
ACCESSION   DADXTG010000261 DADXTG010000000
VERSION     DADXTG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.269"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..401
                     /locus_tag="IWI57_002785"
     CDS             <1..401
                     /locus_tag="IWI57_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450875.1"
                     /translation="KNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYG
                     EDYKNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRY
                     ENGRDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT          434 a          143 c          248 g          388 t
ORIGIN      
        1 gtaaaaactt taccatctat acaggcaaaa atgttataag tagatgggaa aaaggtatta
       61 atattccaga tattgaaaaa ctaatgaata ttgcctatct tggtaaggtc actgttcctt
      121 accttatgta cggagaagat tataagaata tacttccaaa agatgaaagg gttagcgatt
      181 ttaaaaagat aaattctttt agtatgggat taaggatgag gaaaattagg aaagattatt
      241 atctagaaag ggaggagttt ggaaagcttt tttctccttc aatttcaaaa tggtccatag
      301 ataggtatga gaatggtaga gatataccca atactaatcg aattatccaa tatgcttata
      361 taggaaatct ttctttagaa tttttgattt acgggatata aaaatagttt acgtcgatac
      421 ttattaagtt atcgacgttt ttttattata gaaaaaattt ataaaaggag gtttcttgat
      481 atgataagaa acaaaaagtg ttggttgaaa gttgctttac tggcagcttt ttttctaagt
      541 aactttttga caagctcaat ggcaacacaa gcggaagaag taatacaaga aataactgtt
      601 atgaataaca gctagataaa atgggctagg tatgttatcc aacaatttag taatcactag
      661 aagcctagcg aaagttcgct tgataagtta tagatgagta ttgatttgta gtgtttccaa
      721 atcaaaaggg tacagagcca catgaaatat gttgtcctct gcagcgtcag gatgggaatc
      781 cataccatgg agattcggag atctgtgagg gaatacttac gtggttaata tgtcggttgt
      841 gttggacgta gtaatggtca tagtgggtag attgactgaa ttcttatatg agaaaatgaa
      901 accaacaaga agatagggtt atttatacag aattggggat cttttctgga aaagtaatcg
      961 gacaatgcga gattcgtacg gtctttctta aatttctctt aaaaaaaacg gcatcgaatg
     1021 ggttgggtga ttgataagca atgaatcata gactgatata gaataaaaaa tgattctatt
     1081 tatcttttta catctatcgt tttttcaagt aaccgcaaat tgtatcagca ttcaacaatt
     1141 tttgtataca agttctagta aaaggatctg aagaaaaagt tggttgttgt gaagataaaa
     1201 aaagaatcag gtt
//
LOCUS       DADXTG010000262         1209 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.270, whole genome shotgun sequence.
ACCESSION   DADXTG010000262 DADXTG010000000
VERSION     DADXTG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.270"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..735
                     /locus_tag="IWI57_002786"
     CDS             169..735
                     /locus_tag="IWI57_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBC2450876.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            880..967
                     /locus_tag="IWI57_002787"
     tRNA            880..967
                     /locus_tag="IWI57_002787"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:912..914,aa:Ser,seq:cga)
BASE COUNT          418 a          188 c          236 g          367 t
ORIGIN      
        1 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
       61 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
      121 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
      181 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
      241 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
      301 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
      361 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
      421 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
      481 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
      541 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
      601 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
      661 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
      721 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
      781 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
      841 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
      901 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
      961 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     1021 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     1081 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     1141 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     1201 taaatgcta
//
LOCUS       DADXTG010000263         1195 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.271, whole genome shotgun sequence.
ACCESSION   DADXTG010000263 DADXTG010000000
VERSION     DADXTG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.271"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWI57_002788"
     CDS             <1..1076
                     /locus_tag="IWI57_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBC2450877.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DADXTG010000264         1161 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.272, whole genome shotgun sequence.
ACCESSION   DADXTG010000264 DADXTG010000000
VERSION     DADXTG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.272"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..402
                     /locus_tag="IWI57_002789"
     CDS             <1..402
                     /locus_tag="IWI57_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBC2450878.1"
                     /translation="LFYTQIATSEKGFNFDWQTTLFVSVIYFALLLGIVIAYFLLKLL
                     KKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVADLDDAPGLMLVGIAVV
                     FIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            411..638
                     /locus_tag="IWI57_002790"
     CDS             411..638
                     /locus_tag="IWI57_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450879.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          364 a          189 c          192 g          416 t
ORIGIN      
        1 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
       61 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
      121 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
      181 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
      241 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
      301 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
      361 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
      421 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
      481 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
      541 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
      601 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
      661 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
      721 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
      781 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
      841 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
      901 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
      961 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     1021 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     1081 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     1141 aacattattt tgataataat g
//
LOCUS       DADXTG010000265         1152 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.273, whole genome shotgun sequence.
ACCESSION   DADXTG010000265 DADXTG010000000
VERSION     DADXTG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.273"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(512..585)
                     /locus_tag="IWI57_002791"
     tRNA            complement(512..585)
                     /locus_tag="IWI57_002791"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(549..551),aa:Ile,seq:cat)
     gene            complement(775..>1152)
                     /locus_tag="IWI57_002792"
     CDS             complement(775..>1152)
                     /locus_tag="IWI57_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBC2450880.1"
                     /translation="GAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFI
                     MDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDP
                     EGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          211 c          193 g          380 t
ORIGIN      
        1 taggctacct aaaaaggtag cccggaacga attttatcac catacttgtg aaaggagata
       61 ttttttaagt tagtattaag attgtgtaat atgatgatat ctatatttta tagtatcagt
      121 gctataaaat caaaaataag tcactaatta actaccactc caattgtaag ccttttttct
      181 cacttttttt caaaaatatg gtatgctttt taatggcttc aaatataaaa gagtttaaag
      241 cgtaacacac ttatggggaa gtggtttggg gtgcgcttta aactcttctt tattattatc
      301 tcacaattta acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt tcaaatatac
      361 agaagtataa ctatgtgaaa catcctttca ttaatccata aaaggataca taaaaaagcc
      421 actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc aaaaaaattc
      481 taacacagaa cgattcaaat ggctacgtta atgtaccctg taggactcga acctacgacc
      541 ggacggttat gagccgtctg ctctgaccaa ctgagctaag ggtactggtt gttgccacat
      601 aaagccataa acaatcaacc agtagaatgt gtggcaacaa acctgttatc gcatagcttg
      661 gagtgtgact atttatgggt gatagtgaag atatgcgata acatcactat tttatcgaat
      721 aatttttata gttgtcaata tagttatgta ctgctcctca acgaggagct atttttatcg
      781 tttaggaata tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt
      841 tccctctgga tcattcgttg cccgcatcat tacatagact ttcttactag gaaaattacg
      901 catattgaaa gatacatgat aaccaacatt tccagaagta ttataagctt ggtttacatc
      961 tggtctataa attccatcag ctcttactcg agctaattct ttcccagtat tgtagtccat
     1021 aatgaaaata tactcgtatt tatagttagc aatgtgccat ccagccacat gcaagtttgc
     1081 gttttcgatt tccccaaact gatcaatgtg ggcgtgattt gttccatctg tcagcgtagg
     1141 attagctgca cc
//
LOCUS       DADXTG010000266         1122 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.274, whole genome shotgun sequence.
ACCESSION   DADXTG010000266 DADXTG010000000
VERSION     DADXTG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.274"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..817
                     /locus_tag="IWI57_002793"
     CDS             14..817
                     /locus_tag="IWI57_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBC2450881.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            804..>1122
                     /locus_tag="IWI57_002794"
     CDS             804..>1122
                     /locus_tag="IWI57_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450882.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQN"
BASE COUNT          445 a          166 c          213 g          298 t
ORIGIN      
        1 taatataaga tgaatggggg atattttaat ggcaagaata ttgtcagtag ccaatcaaaa
       61 aggtggagtt tctaaaacta cgactagcgt taatttagca gcatgtttga aatttgttta
      121 cggtaaaaat gtacttttag tggacattga tcctcaggga aatgctacag ataatgtagg
      181 tttcgatata gatggaacaa accaacctac aatttatgaa gtattaaaag gggaggctga
      241 cattacagat gctattctgg actataaagg aattgatgtt ttgccagccg acatagccct
      301 ttcttctgca gagagggaat ttactcaagt aggaagtgaa cacaggctta aaagagtact
      361 tcaaccaatc aaggacaact atgattatat tataattgat tgtccaccaa gtttaggaat
      421 gcttacagtc aacgcattta ctgtttctga tgaaattatc attccagttg aagctgcgta
      481 tttctcatta aaaggactaa taaagttaag tgaaactatt gaaacggtaa aagaatatac
      541 gaacgacaag cttgaagtac gaggagtcct atttactaag tataataagc gttataacat
      601 ttctaaagaa atgacgactg cagcaaccga aatttctaga gtcattggtg caaaaatttt
      661 caaaacttat attcgtagaa caataaccgt tgacgaagcg caagctgctg gatcagatct
      721 aatcaatttt aataaaccct caacagctga agatgactat aaagcattta cagaagaata
      781 tttaaaagaa gttggtgatt ttgatggcag aagataatcg cagtcaagct gcagtaagta
      841 gactactagg aaataaaaat aaaaagaact acaaaccaaa agaagtagaa gaaatcgaat
      901 taaccaagaa aaatataagt aaagtcataa aagatcaagg aagtagtgta agaacagcag
      961 atagcattcg tataaatcca attctaatgt cagcactcaa atattggacg actattgctg
     1021 aatcagacaa aagtaaacca gacgttatag aagatgcgct attaaaatcc atacctgaag
     1081 aatacttgat tgaaggatat aaactagcaa agaaacaaaa ta
//
LOCUS       DADXTG010000267         1117 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.275, whole genome shotgun sequence.
ACCESSION   DADXTG010000267 DADXTG010000000
VERSION     DADXTG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.275"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..182
                     /locus_tag="IWI57_002795"
     CDS             <1..182
                     /locus_tag="IWI57_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBC2450883.1"
                     /translation="ESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHK
                     GFQQVTDTLVSMFTE"
     gene            519..758
                     /locus_tag="IWI57_002796"
     CDS             519..758
                     /locus_tag="IWI57_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450884.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          386 a          204 c          167 g          360 t
ORIGIN      
        1 tcgagtcttt caataagcaa attaaaagat acagccgtag aaaagagcag tttcaaaatg
       61 aagaatcact agaacgcttt ctagtcagca tttttgatac atacaatcaa aaatttctaa
      121 acagaagcca taaaggtttt caacaggtaa ccgatacatt agtttcaatg tttactgagt
      181 aactaattat tttgcaggag gacaatttat ttacacaaaa ttattgacgc tcccagctat
      241 cctcctagca actatttaaa tacccaaaaa aattacctct agataatatc atacctacgt
      301 tttcacttat aaattttttc ataaaaaaag cgtttagagg cataatcgtt aatacagatg
      361 gtaaccaatg tatctggttt gaactgggat atcaccctct ccatatatgt attactaaga
      421 ctaatagata ctataaaaga cttgatagtt ttgcattgat tttgcctaat actgagcata
      481 ggtgtatgct aaacgatact ataaggagtg tgtacataat gaaaagaatt ctgtttatca
      541 gtttatcaaa tttattgtta cttactgctt gtgggacttc tgcatcaaag aacatacctt
      601 cagatacgca aatttcgaat agtatcgatt caacaatttc tacaaatgag aaaaattctc
      661 cggtagaaac ttccgatata aaagtttcta cctctgcaaa aaacagggta aagaaactgt
      721 cagcacgtct cctttatttc aaacaaactc tgaattagct tcttcatctt cttcagcaaa
      781 taatgatgat agtttcgcgt ttactcctaa ctaaatgcaa cctttacaaa tgctttacga
      841 taacaagcct gagctaaatg atcctgatat cagttttgca ttttttgaaa tgattggatc
      901 ggatgatctt tttaaaacta ctagtttgtc tatccgaaaa taggacagtt ctggcagtat
      961 aggtttttat cgagtttctc caccaggcct cgttacagag acagattctc taggcgaacc
     1021 ttttgaataa atcaaaaatc atgaacaatc actgccaact aaataaagtg ccttcaaaag
     1081 cttggtcttc tcgcttttga aggcacttta tttaaat
//
LOCUS       DADXTG010000268         1089 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.276, whole genome shotgun sequence.
ACCESSION   DADXTG010000268 DADXTG010000000
VERSION     DADXTG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.276"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            71..907
                     /locus_tag="IWI57_002797"
     CDS             71..907
                     /locus_tag="IWI57_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBC2450885.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            912..>1089
                     /locus_tag="IWI57_002798"
     CDS             912..>1089
                     /locus_tag="IWI57_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBC2450886.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYS"
BASE COUNT          350 a          139 c          235 g          365 t
ORIGIN      
        1 agaaaggcgt tagatattat aaaaaagcat ttacacatta agtagatgtt tattgcgagg
       61 tgaaacaaat atggcaataa aatcttatat taaacggtta ttgctttata tccgtcatgg
      121 agatcccaaa ataaatttag atgtgagtta tttatctgaa ggtgcaatgc ttgctggtaa
      181 gactgtgata attacgggtg gtgcaaaagg acttggtttt gcaattgcaa aatcatttat
      241 agctcaaggc gcaagcgtta ttattacagg aagagacatg gattcgttga ataatgctaa
      301 atgtcagctt ggagatatgt gttctatcag ggcgtttgat ataactgatg ctaaaatgta
      361 tcgaacgttt tttgaagatg catacgagac ctttggacgt attgatatac tgcttaataa
      421 tgctgggatc tcgttacacg aagatggatt tatgagtgtt actgagaaaa cttgggataa
      481 gcaaatggat acaaatttga aaggtgttta tttctttaca caggaattta ttaagtttgt
      541 taaagaaaag aatcaaatgc aagcaaatat tattatgatg tcctcagaaa gaggcttgtg
      601 tggtgatagt attccgtatg gattatcaaa ggctgcaatt aactctttag ttgtaggctt
      661 gtctaagaaa tatatttctg agggaattag ggttaatggt attgctcctg gagtaactgc
      721 aaccgctatg actggtattt ctgaaaaggg taatttgtat aatcctgcaa cgatcggtta
      781 tagatatttt ctcccagaag aagttgctca agttgctttg tttttggcaa gtagtctttc
      841 aaactgtatc agtggtgaaa tcatagcctg tgaccagggg caatatttaa gtcgaaattg
      901 ggggtaataa cttgggaata ttattaaaaa aatataatag ccttagtgag cctgcaaaag
      961 catctatttg gtttacactt tgtaatgtgt tgcaaaaagg aattctattt ttgactacac
     1021 ctatttttac aagaatgctt tcaactgcag aatatggtaa gtatacgatt tttatatcgt
     1081 ggtattcta
//
LOCUS       DADXTG010000269         1086 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.277, whole genome shotgun sequence.
ACCESSION   DADXTG010000269 DADXTG010000000
VERSION     DADXTG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.277"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..>1086
                     /gene="scm"
                     /locus_tag="IWI57_002799"
     CDS             22..>1086
                     /gene="scm"
                     /locus_tag="IWI57_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBC2450887.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT          377 a          194 c          206 g          309 t
ORIGIN      
        1 gaaaggaaag gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct agttgctttg
       61 ttgttcaata tcttttcgta tggtgtggcc gtttcagcgg aaacatacat tcctaaagac
      121 cctgggaata tttataatga tctaactggt aactatggct tgttaggaat tgctagtcaa
      181 ttccacgtat ttgctaaaaa taagacaact gtgaatgcac atacagacgg aaatatagca
      241 acacatgaac ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg tttattgaac
      301 caagaaataa attatataca aaaaactgat tctttcgcta attcttcagg catacctaca
      361 ggtagcacaa gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc acttcaagat
      421 ggtcatccta cagtcaatgg atccaggctt gatcacttaa ctccagatga tttttataaa
      481 gatcgtgctg ggcatgaata tattgatttc gagcaggaat ttgctaaatt gaatgtagca
      541 gcaaatgatc ttgcaacgat cactccagct aaaacatata ctgctgcaga ttttccagac
      601 atgaacaatc ggacaatcga tttaactgat ttatcagata ccgggttttt attggtaaat
      661 atcgatgcag aagtgttaac gatgaatacg ccacttcaaa tcattaatcc aaatgatcaa
      721 gtagttgtat ttaatgtcat caacagttct tctgcattga acgtacagtc accaatcaaa
      781 tacaatgatc gttcaaatca tgaaacagaa gatttttcgg atgcgaatat ctcttggaat
      841 tttggaaatg agatgactga tttgacaatc agtgctcctt ttcaaggaac aatccttgcg
      901 cctaatgcta ctatccgcat tactcaaaac caagacggaa cgattatagg aacaaatgtg
      961 attttagatg cagctaccaa tcgttgggat cctaacgaaa tatttatcag cgacaatggt
     1021 acagatacga cagacacaag cgacagtaca gatacatcta ccacaagtga tagctcagat
     1081 tcatca
//
LOCUS       DADXTG010000270         1071 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.278, whole genome shotgun sequence.
ACCESSION   DADXTG010000270 DADXTG010000000
VERSION     DADXTG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.278"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(88..>1071)
                     /gene="uidA"
                     /locus_tag="IWI57_002800"
     CDS             complement(88..>1071)
                     /gene="uidA"
                     /locus_tag="IWI57_002800"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBC2450888.1"
                     /translation="IRSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKD
                     FNLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEK
                     HDTWKEIRTENAHKMAIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTK
                     ELDPQKRPVTIVTYLKSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELD
                     GWMERCPDTPIMFTEYGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQ
                     VWNFADFETSQGILRVQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT          323 a          223 c          138 g          387 t
ORIGIN      
        1 cttctttaaa cagaaaatct ttttataaaa aaaaggagtt ggcaattaag cagccactcc
       61 ttttttatat tccttataaa aggtaaatta ttttttataa ctgaaatttg gtattgatga
      121 ccatcgtttc tttagatagt gagcaatcat tttaggcttt ctatctctag taaagattcc
      181 tttcttattt ccttgcactc ttaaaatccc ttgacttgtt tcaaaatctg caaaattcca
      241 cacttgttca ccaacaaaat tgtcacattt atctattact tcgtgatttg cttcgtaata
      301 acccacttga tactcttctg taaacattgt tggtactgta tcatgaagtc cagctactgt
      361 atctgctcca tattctgtaa acattattgg cgtatcggga catctttcca tccatccatc
      421 tagctctttt ttcaacagat attttgcatg ttttaggtct ccacctgaaa cataccaacc
      481 ataatagcga tttaaacata aaacatcgac aatatctcct actttacaaa tatctggtgt
      541 tgactttaag taagtaacga ttgttacagg tctcttctga gggtcaagtt ctttagtcaa
      601 ttcaactaaa ggttcaaaat attctttagc gccttcagaa tccgaatctg gttcatttgc
      661 aatagaccac attacgacac tcggatggtt cttgtcgcgt tctatcaatt ccttaatcgc
      721 cattttgtga gcattttctg tccgtatttc tttccaagta tcatgttttt ctacgttgtc
      781 atctaataaa gtagccatga aattcaaatg taatcctact gcaggtactt catcaataac
      841 tacaatacct tctcgatctg ctaagcgcat aatttcttct gaataaggat aatgtgaagt
      901 cctaaatgaa ttagcaccga tccatttcat taaattaaaa tctttgatat ttactgcttc
      961 attcaaacca cgaccattaa tatacgtgtc ctcatgcttc ccaaatccct taaagtaaaa
     1021 aggtttatta ttaatcaaaa acttcccatt cgaaacctga atactacgaa t
//
LOCUS       DADXTG010000271         1065 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.279, whole genome shotgun sequence.
ACCESSION   DADXTG010000271 DADXTG010000000
VERSION     DADXTG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.279"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(585..994)
                     /gene="tnpA"
                     /locus_tag="IWI57_002801"
                     /pseudo
     CDS             complement(585..994)
                     /gene="tnpA"
                     /locus_tag="IWI57_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          307 a          192 c          141 g          425 t
ORIGIN      
        1 ttttttcatt tattatctat tattctcttt taaaaatagc cccaatgatc ttatgtaagt
       61 aaacggacgg acaatttata atatagcact ttgcttcttg ttgcttgtat ggttattttt
      121 gtgtttaaaa tctaaaagtc taactaaaat tctaccttta aatttcagat tcttgaatgc
      181 ccaatttata tagagcacta tcatgcttct tttgttcttg gacgcaattt cttctggtct
      241 attcattcaa tcctagtgta tttacacaat attcctttgg cctaaaactt tgataaatac
      301 gtatatataa atttacgtat ttatcaaaaa tataaatgca atttttactt tcagataact
      361 cttaaaacaa cctactatca ggtagtttta agagttcact cactttgcga atccaagcgg
      421 gtcttgaagt aataatagta aaatccccta aaaaaattgt cattctatag acccttaaat
      481 ggacttatct ccaaaagtta aactattttt cggtatttta ctggaggtag tccattttta
      541 cccttttgtt attgcaacat ctactctctt ttacttatac tatctcaaat gcgatcatca
      601 gcttcttgtt cacggatgta tttttcaatt gtcttttgat tcaattcgac tgtactcaca
      661 taatattcct ccgaccaaaa tgatttattt ccgtatctat atttgtgatt tacgtgctgc
      721 tcgtggatta gcacagcact acgatctttc agataactat aaaactggat atacttattt
      781 ttgatggtac ttgaacaagc atgtgaatat gatctggcgt tgcatgtgct tctataattt
      841 ccacgttttt tattttgcac aacttcctaa gtatttttcc aatatctttt cgtaattgcc
      901 tatagattac ttttcgtcga tattttggtg taaatacaat atgatactta aaattccaac
      961 gagtatgggc taaactttta tcgtcatttg acatgaaaaa actcctttcg atatgctagt
     1021 tcttccacgt gtagaaacgc atggttttct tgtttcgcac gcttt
//
LOCUS       DADXTG010000272         1058 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.280, whole genome shotgun sequence.
ACCESSION   DADXTG010000272 DADXTG010000000
VERSION     DADXTG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.280"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            54..389
                     /locus_tag="IWI57_002802"
     CDS             54..389
                     /locus_tag="IWI57_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450889.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            622..>1058
                     /locus_tag="IWI57_002803"
     CDS             622..>1058
                     /locus_tag="IWI57_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBC2450890.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLN"
BASE COUNT          385 a          161 c          219 g          293 t
ORIGIN      
        1 gtccaaagca atgagtcaca aaccaaaaat tatacctaag gagattttat cacatgaata
       61 agaaaattga aaatcttatc gaagaattga aacgtgaatg ccaaaagcaa ggtgtttcta
      121 ttatttgtac agcccaaaaa gaaggggaac ttaaaagtct cgtttatggt gagacaacag
      181 aaattttact ttgtcttgct atgcaagaag aacatttaga tgaaaatctc ccattgtcag
      241 cacatattat gcgtagaatt gctgtggatg cttacgaaca agcgaaaaat gaagaagaaa
      301 atcaaccttc aaatcatact tttgttatta ataacaagga agatttagct gatgtgatga
      361 ctcgtattct caagggggaa tttcaatgat gaatgaaaaa acaattgatc tactcattat
      421 tttatttttc acgttagtga ttgtagcgaa aagttatgta atctttggct tgttattcgc
      481 aattaccatg cttgtgcttt gcgtgttagc aagcagaaaa gaggttaaac atgaacgagt
      541 tagcaaatct tgacaactat ttaactgatc ctgattatgc taaaccacca tatgaagcgc
      601 caattgatga ggaggatgaa gatgagtaaa tctaccttag aaatgagcca tcaagaatgg
      661 ctcgaagacc gcaaaagagg tattggtggc tcagatgttg caaccgtact tggattaaac
      721 aaatacaaat ctccttatca attgtggctt gagaaaacgg gtcaaattga attgaaagat
      781 ttagaaagcg aaccagctta ttggggtaat gttttagaag aagtggttgc taaagagttt
      841 caggaacgta caggcaaaaa agtacgcaga agaaaccaag tatttgaaca tccattacat
      901 ccatttttaa gagcaaatat tgatcgggaa gtagtgggag aaaatgccat tctggaatgt
      961 aaaacagcca atcaatttct cggaaaagag tgggaaggtg aagaggtacc gcttagctat
     1021 ctctgtcaag ttcagcatta catgaacgtt ttaaacaa
//
LOCUS       DADXTG010000273         1057 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.281, whole genome shotgun sequence.
ACCESSION   DADXTG010000273 DADXTG010000000
VERSION     DADXTG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.281"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..752
                     /locus_tag="IWI57_002804"
     CDS             <1..752
                     /locus_tag="IWI57_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBC2450891.1"
                     /translation="IHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQQ
                     EILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPEN
                     MSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVFTM
                     EDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHAS
                     YSTTANLYTHLTAGVKDRAAEALENAFGFKE"
BASE COUNT          402 a          149 c          181 g          325 t
ORIGIN      
        1 ttattcatta tcacgcattt atacgcaaag ctcttcaaga ggctgttatt acagaattga
       61 taagtacaaa tgtagcagat aaagtaagaa agccaaaaaa gaaacaattt gtttctcaag
      121 tttataatca acaagagatc ttgaaattac ttgacatttt gtcagaagaa aaactctatt
      181 tggtagttct tttaacagca ttttatggat tgcgaagaag tgaagtttta ggattaaaat
      241 ggtctgctat tgatttttta aacaaaaaaa tcatgataaa ccatgtaatc atagaaaatc
      301 cagagaatat gtcacagtta ataaagaaag atcaaacaaa gaactcttcc agctatcgta
      361 cattaccttt ggttgaaacg attgaacgag cattagtaaa tcaagctaaa tggcaacaag
      421 ataatcgtat tcttttagga gatgattata ttttagaaga taatgagtac gtatttacta
      481 tggaagatgg acggttgatg aagcctcaat atgtaacgca tcgtttgagt aagttgatta
      541 agaaaaatgg attgaaaaaa attcgctttc atgacttgcg tcattctaat gcttcaataa
      601 tgttagatag tggccagaat atgaaatcca ttcaagaatg gctgggacat gcatcgtatt
      661 cgacaactgc taatttatac acgcatttaa ccgcaggagt aaaagatcgt gcagcagaag
      721 cattagaaaa cgcatttggg tttaaagaat aaaaaaacaa aaaacaatat ctttggggta
      781 tcgcttcttg tttttagaat ttttttgttg gcaatttgtt ggcagtatgt tggcaatgaa
      841 aaaattcaca aactaaaaac ctatttataa agctttataa aacttttttc acaaatagag
      901 catttggtta agagtcagct gctctaccaa ctgagctaac ggcccgtaca aaaagtactt
      961 aataatagta gcactaagaa ttttaaaatg caagactaca tttaaagaaa atcagaaaaa
     1021 tctttacttc cttataggaa atcattcata gattatt
//
LOCUS       DADXTG010000274         1052 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.282, whole genome shotgun sequence.
ACCESSION   DADXTG010000274 DADXTG010000000
VERSION     DADXTG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.282"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..885
                     /locus_tag="IWI57_002805"
     CDS             <1..885
                     /locus_tag="IWI57_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBC2450892.1"
                     /translation="QRNEKALMASMLEMYVSGVSTRKVSTIVEELCGKSVSKSFVSSL
                     TEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREI
                     IGFMIQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQV
                     HFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASL
                     DDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLM
                     DLHDEWIYSSRKYINFDK"
BASE COUNT          384 a          182 c          192 g          294 t
ORIGIN      
        1 caacgaaacg aaaaagccct catggcttca atgttggaaa tgtatgtatc aggcgtttca
       61 actcgtaaag tatcaacaat tgtggaagaa ctttgtggta aatccgtctc taagtccttc
      121 gtttctagct taacagaaca gctagaacct atggttaacg agtggcagaa tcgtttatta
      181 tcagaaaaaa attatcctta cttaatgacc gatgtactct atataaaagt acgagaagaa
      241 aatcgagtac tctcaaaaag ctgtcatata gcgattggaa taaccaaaga tggcgaccgt
      301 gaaattatcg gcttcatgat tcaaagtggc gaaagcgaag agacctggac aacatttttt
      361 gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga tgcgcacaaa
      421 ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag atgccaagtt
      481 cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc tttcagagaa
      541 gctgttaaag gaatttttaa gttcacagat attaacttag cgcgtgaggc taaaaatcga
      601 ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc attggatgat
      661 ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg actaaagagt
      721 accaatctaa ttgaacgact gaatcaagaa gtacgcagaa gagaaaagat tattcgcatc
      781 ttccccaatc aaacatcagc caatcgctta attggagccg ttcttatgga cctacatgat
      841 gaatggattt attcttcaag aaaatacatc aattttgata agtagaaatg gtaaaaacat
      901 tgtatagcat tttacacagg agtctggact tgactaaaat ttctaacatc agttattatg
      961 ataattttga tgatgtcaat tatgatttac aggaaagata taatgattta atcgaagaat
     1021 tagaggagag aggagaatga tttcagataa ta
//
LOCUS       DADXTG010000275         1050 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.283, whole genome shotgun sequence.
ACCESSION   DADXTG010000275 DADXTG010000000
VERSION     DADXTG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.283"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1050
                     /locus_tag="IWI57_002806"
     CDS             <1..>1050
                     /locus_tag="IWI57_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBC2450893.1"
                     /translation="YALTSSFLGLSSFISLCALMLYLPFRTVINGLLDLSTEFVILIL
                     IESIFEPAFLLWSVKERFDYKYQKLVLITVLMAVLNPGLGIILILSFGEYAIWRVRAY
                     VVVNVVVGMFIGIGILAKGKKLFDKEYWTYALRFNLPLIPHYLSMTLLNQVDRLMINS
                     MVGASEAAIYSIAYTISSMMNIITTAINGSFIPFTYKAIKDGVYEKIKSVANQLLVLI
                     FVLCSFTMLFGPELIWVVGGEKYMDAVWIIPPVAASVFFMFLYPLFGNIEFYYEKTNY
                     VMFASCVGSIAKIISNYVSIPRFGYISAGYTTLICYMLFSFAHYMFYKKILRENGIKE
                     DMYNIKNIIFLSVLM"
BASE COUNT          299 a          149 c          201 g          401 t
ORIGIN      
        1 atatgcatta acctcgtcat ttttaggatt atcgtcattt atttcccttt gtgcattgat
       61 gctatacctt ccatttagaa cggttataaa tgggctactg gatttatcga cggagtttgt
      121 catattgatt cttattgaat caatatttga gccggctttt ttgctttggt cagttaagga
      181 acgatttgat tacaagtatc aaaaactagt actcatcaca gttcttatgg ctgttttaaa
      241 tccaggtctc ggtattatat taatcttatc atttggggag tatgctattt ggagagtacg
      301 agcttatgtc gtggtaaatg ttgtagtggg gatgtttatt ggcataggaa tactggctaa
      361 gggtaagaaa ttatttgata aagagtactg gacatatgcg ctcagattta atttgccatt
      421 aataccacat tatttatcaa tgacattgct gaatcaggtt gatcgcctta tgataaatag
      481 tatggttgga gctagcgagg ctgcgattta tagtatcgct tacactatat ctagtatgat
      541 gaatatcata acaacagcta taaacggttc ttttattcct tttacataca aagcaattaa
      601 agatggcgtg tatgaaaaaa ttaaatccgt tgctaatcaa ctattagtgt tgatttttgt
      661 cttgtgttcg tttactatgc tgttcgggcc agaattgatt tgggttgtcg gaggagaaaa
      721 atatatggat gctgtttgga ttattcctcc agttgcggca tcagtatttt tcatgttttt
      781 atatccgctt tttggcaata ttgaatttta ctatgaaaag acaaactacg taatgtttgc
      841 atcttgtgtg ggctctatcg caaaaattat ttcaaactat gttagtattc cccgttttgg
      901 atatatttcg gctggttaca cgacattgat ttgctatatg ttgttttcct ttgcccacta
      961 catgttttat aaaaaaattc ttagagaaaa tggtataaaa gaagatatgt ataatattaa
     1021 aaatattata tttctttctg tcttaatgat
//
LOCUS       DADXTG010000276         1035 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.284, whole genome shotgun sequence.
ACCESSION   DADXTG010000276 DADXTG010000000
VERSION     DADXTG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.284"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWI57_002807"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWI57_002807"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DADXTG010000277         1034 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.285, whole genome shotgun sequence.
ACCESSION   DADXTG010000277 DADXTG010000000
VERSION     DADXTG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.285"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..148
                     /locus_tag="IWI57_002808"
     CDS             <1..148
                     /locus_tag="IWI57_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBC2450894.1"
                     /translation="EKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRY
                     SLGY"
     gene            complement(303..746)
                     /locus_tag="IWI57_002809"
     CDS             complement(303..746)
                     /locus_tag="IWI57_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450895.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
BASE COUNT          381 a          195 c          154 g          304 t
ORIGIN      
        1 agaaaaatta ggttgtcaaa atttcaactc acgttctgtt aaagggctag agagtagatt
       61 tgaaagcata ttactggctt acagtgttct attaagtcga gcacaacgac gttttgaagg
      121 aactttgaga tattctttag gatattaaaa ttaactagca ccacgggtgt aatcatttag
      181 ttttatctcc tttttaccaa ccatctacca ctaggttacg aactacctaa gtgcccacca
      241 tccgccgcgt ggaggctgtt aatcaggatg gcaaccgccc ggtagaggcg acaattatgc
      301 tatcatttta atttaagtct atataacata atactataat aatatgacaa caccaacgtt
      361 ataggaatgg aaacaaatgc cgccaaaacc aagttaggga taatttgatg aatcgtacta
      421 aatacaagaa tatggagtag atatatatca tatgagtatc ttgaaatcct aggcattatc
      481 tttctaaatg gaataacttg atacattaaa attgcaattg caccgtaacc gaagaatgca
      541 aaaatatcat taatagactc catcagtcca ccaataattt tcgatatacc agcaaaacca
      601 attccaacaa tggacgccat aatcacgaat atattattaa atcttactat ttttcttttc
      661 actaaatact ctgcaaccaa acatgccgaa agtaaattcc caaatatatt gcgcaaagca
      721 gctcccaaga actcgtatgc ttccatatcc tgtatgaaac atcataatag cccagatact
      781 aataactcag cttgcaacaa gcaatcattt cattcctatt tttctctata gttttacaat
      841 tggtatgaat aagaaataga actgaataat tgtcgaaata aaccaaaggg acccagtgac
      901 tcctcatata ctggtagaaa aattttaaat aacaaaacat gagacaaata tacttttgca
      961 gcatttaaat cttgtgtcat ataaggtagt aacactgata ccgtaattac gataatatac
     1021 ggaatataaa tctt
//
LOCUS       DADXTG010000278         1021 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.286, whole genome shotgun sequence.
ACCESSION   DADXTG010000278 DADXTG010000000
VERSION     DADXTG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.286"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWI57_002810"
     CDS             complement(55..789)
                     /locus_tag="IWI57_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBC2450896.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          360 a          209 c          183 g          269 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 t
//
LOCUS       DADXTG010000279         1010 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.287, whole genome shotgun sequence.
ACCESSION   DADXTG010000279 DADXTG010000000
VERSION     DADXTG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.287"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1010
                     /locus_tag="IWI57_002811"
     CDS             82..>1010
                     /locus_tag="IWI57_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBC2450897.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAP"
BASE COUNT          348 a          182 c          194 g          286 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa
//
LOCUS       DADXTG010000280         1009 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.288, whole genome shotgun sequence.
ACCESSION   DADXTG010000280 DADXTG010000000
VERSION     DADXTG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.288"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWI57_002812"
     CDS             <1..848
                     /locus_tag="IWI57_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBC2450898.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          329 a          195 c          202 g          283 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaatttta
      961 tttatggtat gacataaata aagtaaagtt attcttctag gttaatctt
//
LOCUS       DADXTG010000281         1000 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.289, whole genome shotgun sequence.
ACCESSION   DADXTG010000281 DADXTG010000000
VERSION     DADXTG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.289"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            346..981
                     /locus_tag="IWI57_002813"
                     /pseudo
     CDS             346..981
                     /locus_tag="IWI57_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          340 a          182 c          180 g          298 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttacctt atattatttg tcaaattaag
//
LOCUS       DADXTG010000282          999 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.290, whole genome shotgun sequence.
ACCESSION   DADXTG010000282 DADXTG010000000
VERSION     DADXTG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.290"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>999
                     /locus_tag="IWI57_002814"
     rRNA            <1..>999
                     /locus_tag="IWI57_002814"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          310 a          198 c          287 g          204 t
ORIGIN      
        1 ctatgatcca gagatttccg aatgggggaa cccaacatct ttaataggat gttacgattg
       61 tgtgaataca tagcacattc gaggtagacg cagagaactg aaacatctaa gtacctgcag
      121 gaagagaaag aaaattcgat tccctgagta gcggcgagcg aaacgggaaa agcccaaacc
      181 agcaagcttg cttgttgggg ttgtaggact ccaatatggt agttctttca gatagtcgaa
      241 tgacttggaa aagtcagtca aagagggtaa aaaccccgta gacgaaatgt ggaagacacc
      301 taggaggatc ctgagtacgg cggaacacga gaaattccgt cggaatccgg gaggaccatc
      361 tcccaaggct aaatactccc tagtgaccga tagtgaacca gtaccgtgag ggaaaggtga
      421 aaagcacccc ggaaggggag tgaaatagaa cctgaaaccg tgtgcctaca acaagtcaaa
      481 gcccgttaat gggtgatggc gtgccttttg tagaatgaac cggcgagtta cgattgcatg
      541 cgaggttaag ttgaagagac ggagccgcag cgaaagcgag tctgaatagg gcgtttgagt
      601 atgtagtcgt agacccgaaa ccatgtgatc tacccatgtc caggttgaag gtgcggtaaa
      661 acgcactgga ggaccgaacc cacgtacgtt gaaaagtgcg gggatgaggt gtgggtagcg
      721 gagaaattcc aaacgaactt ggagatagct ggttctctcc gaaatagctt tagggctagc
      781 ctcggaattg agaatgatgg aggtagagca ctgtttggac taggggccca tctcgggtta
      841 ccgaattcag ataaactccg aatgccattc attcatatcc gggagtcaga ctgtgagtga
      901 taagatccat agtcgaaagg gaaacagccc agaccaccag ctaaggtccc aaaatatatg
      961 ttaagtggaa aaggatgtgg ggttgcacag acaactagg
//
LOCUS       DADXTG010000283          991 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.291, whole genome shotgun sequence.
ACCESSION   DADXTG010000283 DADXTG010000000
VERSION     DADXTG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.291"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IWI57_002815"
     tRNA            complement(21..94)
                     /locus_tag="IWI57_002815"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IWI57_002816"
     tRNA            complement(108..181)
                     /locus_tag="IWI57_002816"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IWI57_002817"
     tRNA            complement(209..281)
                     /locus_tag="IWI57_002817"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IWI57_002818"
     tRNA            complement(301..374)
                     /locus_tag="IWI57_002818"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IWI57_002819"
     tRNA            complement(384..457)
                     /locus_tag="IWI57_002819"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IWI57_002820"
     tRNA            complement(468..553)
                     /locus_tag="IWI57_002820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IWI57_002821"
     tRNA            complement(570..641)
                     /locus_tag="IWI57_002821"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IWI57_002822"
     tRNA            complement(653..725)
                     /locus_tag="IWI57_002822"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IWI57_002823"
     tRNA            complement(743..824)
                     /locus_tag="IWI57_002823"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="IWI57_002824"
     tRNA            complement(832..904)
                     /locus_tag="IWI57_002824"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="IWI57_002825"
     tRNA            complement(908..980)
                     /locus_tag="IWI57_002825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DADXTG010000284          982 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.292, whole genome shotgun sequence.
ACCESSION   DADXTG010000284 DADXTG010000000
VERSION     DADXTG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.292"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            341..964
                     /locus_tag="IWI57_002826"
     CDS             341..964
                     /locus_tag="IWI57_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2450899.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          364 a          135 c          179 g          304 t
ORIGIN      
        1 attccttact aaattagttt ctgaatggaa aagtattatt tctcaaaata tggataaatt
       61 aagatattaa ggatgttagt tttaaaataa actaatgtcc ttatttttgt gttcacattt
      121 ctgttaccta agaaatactt aaaggaggtg atgtagccag caacgactac aacttgaacc
      181 cgctatctac agtttatttg ttgtttcttt tgggtatact ctaatggtac aaatccatat
      241 ttgcttaaag cgttgatttt tcagcggttt ttctttattg caaagtgtac cctaagacct
      301 gtatactaat agtaaataga ttggatagga atgatggcaa atgtatttag cttatttaag
      361 agtttcttcc gacgatcaaa gtttagctcg gcaacataag ctaattgaaa attggtcagt
      421 caaaaataat attgctgaag acgatttaaa aattttcgag gaaaaagtct ctggaaaaaa
      481 tattgaaaac agaccagagc tgcaagagct tatttctttc atacgtgaac gagatacgat
      541 tattattcct agtcttgatc gattagcaag aaatagtaaa gatacaaaag acttacttca
      601 attgtttcgt agtaaaggtg cttctattga aatattagac ctaccgtcgt ttaatggggt
      661 aactgaccct gcattaagag acttgctaac aaatttagtc atagaagtgt ttagctatgt
      721 tgctgaaaac gaacggaaaa aaatcaaaga gcgtcaaaaa caaggcattg aaattgcaaa
      781 gaaaaatggt cgttatactg gtaaaaagat tcaataccat gccaatgcaa caggaaaaaa
      841 caaattgatt tacgatgaaa ttgttgatgg tctgaagaaa aaagaaacta tttctaaaat
      901 agcaaagaac gcaggagtta caagaaaaac cgtttatcga gtaaaaagag atttaggaat
      961 gtaatatgtt ccagttagaa ag
//
LOCUS       DADXTG010000285          958 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.293, whole genome shotgun sequence.
ACCESSION   DADXTG010000285 DADXTG010000000
VERSION     DADXTG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.293"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..774
                     /locus_tag="IWI57_002827"
     CDS             <1..774
                     /locus_tag="IWI57_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBC2450900.1"
                     /translation="DSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYI
                     VKVNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASAD
                     VEKELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMT
                     RRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFF
                     KVSLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
BASE COUNT          420 a          124 c          169 g          245 t
ORIGIN      
        1 gattcaacta gagaaaaaat tataagaact tttgataaaa taaacaagct ttttaaaaac
       61 gatgaagcag aattcaaaaa agaattactt gaattaaaac ttcaagaatt agaactcaac
      121 tattcataca tagttaaagt taacgatgaa aaagaacagc aaaaagcaat aaaagaacaa
      181 atgattgaag aagaaaaggt aagacgtgaa atagaacgtg aaaagaaacg cattgataaa
      241 gaagaacgac agtttaactc agagataact aagttaatgg catacatgca aaaagctagt
      301 gctgatgttg aaaaagaact ctatgctgat aaaattaaag aactggaaga gaaattaaga
      361 gaactagaaa ctgtcaaaga agatgtattg caaagagaac ttaatactag agctggctat
      421 gtttatgtca tcagtaatat cggatctttc ggtgaagatg tctataaaat aggaatgact
      481 agacggttag aacctatgga tagaattaag gaacttagta gcgcttctgt accttttgaa
      541 tttgatgttc atgcaatgat attttcagaa gatgcaccaa cattagaaaa tcaactacat
      601 tcgcatttta gaaaaaacga agtcaataaa gttaatcaac gaaaagaatt ctttaaagta
      661 tctcttgatg aaattgaaaa agttgtttta gaaaattata acggaactgt atcttttact
      721 aaactagctc aagctgaaca atacagaaga tcattagaat taacagatgt gtagtaaaaa
      781 gattagcctt cgggcttttc ttttacaacg ataagaacat acattcgaaa ggagataaca
      841 tgaaacgagt agcattatat atgagagtat caactgaaca acaagccaaa catggagata
      901 gtcttagaga acaaaaagaa accctctatg aatatatcga acaacacaag gatttgaa
//
LOCUS       DADXTG010000286          933 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.294, whole genome shotgun sequence.
ACCESSION   DADXTG010000286 DADXTG010000000
VERSION     DADXTG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.294"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWI57_002828"
     CDS             <1..106
                     /locus_tag="IWI57_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBC2450901.1"
                     /translation="PRLGVFTLESPSGSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(220..849)
                     /locus_tag="IWI57_002829"
     CDS             complement(220..849)
                     /locus_tag="IWI57_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBC2450902.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          309 a          145 c          156 g          323 t
ORIGIN      
        1 tccacgtcta ggtgtattta ctcttgaaag cccaagtggt tctttagaca gtcatgttca
       61 attagtagat ctacgaccag aaaaattctc tggaataaaa caataacttt ctctctattg
      121 tccataaaac tagtctcaat caagctgtat tgaaaaaaca agacaacatt cttaccatgg
      181 taaataaaaa gacgacacat tttgtcgtct ttttttaaat taactatttt cgataaaaga
      241 tcgcatagct tgtgccatta tttctggctt ttctctattc aattcatgtt ttccgttttt
      301 aataaagagt agccggccat tcgttgaaag tttatataaa cgttttgctg ctggtttatt
      361 cgcatgatct ttggtaccgc ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt
      421 tttgcagtct aatgtttcaa gtgaacgaag tagggtaagg gtttgttttt tagatagacc
      481 taatttttca aatatcttag aaggtacgca gtaaaaataa atattctgga aagtaagcaa
      541 taagcgatta ggagactcaa attgtggagc gactaaaaca acctttttta aatatggaga
      601 aggagttgca agatagttta aagcaataac agcgcctaaa gataatccac ataaaataaa
      661 aggttccttt atctgattca attgtacttc gatgtatttg gttaagtcgt ctaaagtagc
      721 agtgtctgtg ctgtttaaat gattgaacaa tgaaagactt ttgcaagaat aactattggg
      781 tagataagag attacatctt tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac
      841 aattttcata ttgctctcct ttttatagtt atcatataga ctttctttgt actattaaaa
      901 tataaacatt catttcttac gtcataaaat aaa
//
LOCUS       DADXTG010000287          926 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.295, whole genome shotgun sequence.
ACCESSION   DADXTG010000287 DADXTG010000000
VERSION     DADXTG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.295"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            65..802
                     /gene="erm(B)"
                     /locus_tag="IWI57_002830"
     CDS             65..802
                     /gene="erm(B)"
                     /locus_tag="IWI57_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBC2450903.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          349 a          157 c          143 g          277 t
ORIGIN      
        1 attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact tcaggagtga
       61 taacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
      121 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
      181 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
      241 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt
      301 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
      361 gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccgtgc
      421 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
      481 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
      541 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
      601 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
      661 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
      721 agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct
      781 attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg
      841 ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa ctaccaatta
      901 attcacctca aaatgaaatt agaaaa
//
LOCUS       DADXTG010000288          898 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.297, whole genome shotgun sequence.
ACCESSION   DADXTG010000288 DADXTG010000000
VERSION     DADXTG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.297"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..843
                     /locus_tag="IWI57_002831"
     CDS             <1..843
                     /locus_tag="IWI57_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HBC2450904.1"
                     /translation="YTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMGFNKQLGKN
                     NPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAESLGAQEGM
                     KIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFETIMTNDSIA
                     TFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDSYYVNAEQE
                     LYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYESSPSTITK
                     DWLE"
BASE COUNT          375 a          138 c          153 g          232 t
ORIGIN      
        1 tatacgacaa atgaactatg tagagtatta aactgccaaa aacaaaaagc gatagcaatc
       61 aaaaaagagt tggaatcctt tggtttatta ttacaaaagc agatgggatt taacaaacag
      121 ttagggaaaa ataatcctaa tagactatat ctagcagaat taaaagtctc agaaaatgat
      181 atctacttac tcgaaaaatt tgatagagag aatagggaaa acgttgataa atcagagggt
      241 atgaaaatca tacccaccct cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag
      301 ggtatgaaaa tcataccgtg ccaaaacgtt gataaatcag agggtatgaa aatcatacca
      361 gaacttaata ataatatatt agacactaat agacacaata tagacactga aaaagaccgc
      421 ctacaagatc aattgttgtt agacaatttt gagacaatta tgacaaacga cagcattgct
      481 acgtttgtcc ctgaacgagt attaaatttg ataaaaacat tttcttcaag ttacagtgaa
      541 gctcaaaaaa ccgtccagac tattcataat gcaaagaaaa aagctgaaat agaaagtggt
      601 atttcgatag tttttgaaga actcgatagt tattatgtca atgcagaaca agaattatac
      661 acgacactgt taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac
      721 ctgatttttg tctatgtaaa aaattggttt atcgaaaaac caatagctgc taaagtatca
      781 agtgaaaaac gtttgaatta tgaaagctcc ccaagcacta ttacgaaaga ctggttagag
      841 tgaaacttta ctgacaaagt gattgttgct tcatggagta acattaaaat taaaaatt
//
LOCUS       DADXTG010000289          893 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.298, whole genome shotgun sequence.
ACCESSION   DADXTG010000289 DADXTG010000000
VERSION     DADXTG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.298"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>893
                     /locus_tag="IWI57_002832"
     CDS             <1..>893
                     /locus_tag="IWI57_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBC2450905.1"
                     /translation="TRIGTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSG
                     VSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIK
                     VREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELV
                     ISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDIN
                     LAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQE
                     VRRREKIIRIFPNCVSANRLI"
BASE COUNT          318 a          172 c          162 g          241 t
ORIGIN      
        1 gacacgtata ggcacgctag aattaaaagt acccagaaca cgtgatggcc atttttcacc
       61 cacagtgttt gaacgttatc aacgaaacga aaaagccctc attgcttcaa tgttggaaat
      121 gtatgtatca ggcgtttcaa ctcgtaaagt atcaaaaatt gtggaagaac tttgtggtaa
      181 atccgtctct aagtccttcg tttctagctt aacagaacag ctagaaccaa tggttaacga
      241 gtggcagaat cgtttattat cagaaaaaaa ttatccttac ttaatgaccg atgtactcta
      301 tataaaagta cgagaagaaa atcgagtact ctcaaaaagc tgtcatatag cgattggaat
      361 aaccaaagat ggcgaccgtg aaattatcgg cttcatgatt caaagtggcg aaagcgaaga
      421 gacctggata acattttttg aatacctaaa agaacgcggt ttacaaggta cagaactcgt
      481 tatttctgat gcgcacaaag gattagtctc tgccattaga aaatccttca ccaacgcaag
      541 ttggcaaaga tgccaagttc acttcctaag aaatatcttt accaccattc ctaaaaaaaa
      601 ttcaaaatct ttcagagaag ctgttaaagg aatttttaag ttcacagata ttaacttagc
      661 tcgtgaggct aaaaatcgat tgattcatga ttatatcgat caaccaaaat attcaaaagc
      721 ttgcgcatca ttggatgatg gattcgaaga cgcctttcaa tataccgtac aaggaaattc
      781 ccacaatcga ctaaagagta ccaatctaat tgaacgactg aatcaagaag tgcgcagaag
      841 agaaaaaatt attcgtatct ttccaaactg cgtttcagcc aatcgtttaa tcg
//
LOCUS       DADXTG010000290          873 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.299, whole genome shotgun sequence.
ACCESSION   DADXTG010000290 DADXTG010000000
VERSION     DADXTG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.299"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..259
                     /locus_tag="IWI57_002833"
     CDS             <1..259
                     /locus_tag="IWI57_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBC2450906.1"
                     /translation="IRSDGGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLVQD
                     IATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            315..533
                     /locus_tag="IWI57_002834"
     CDS             315..533
                     /locus_tag="IWI57_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450907.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            604..>873
                     /locus_tag="IWI57_002835"
     CDS             604..>873
                     /locus_tag="IWI57_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450908.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKH"
BASE COUNT          335 a          105 c          169 g          264 t
ORIGIN      
        1 tattaggtca gacggaggat taatggcatc accttacttg ccaattcaag ttggaaatat
       61 taaatgtcac agtctttcta aatattggcg agcaggactt tataaagttt tttctttaga
      121 tttagttcaa gatatagcta ctcaagtttc ttcaatacaa gatatgagta aagaattaaa
      181 aaaaggacat attaaatggc ttaatgaaga tatagaaatt gatattctcg atgaatacaa
      241 acatgatatt ttaatttagg tttatcattg gaaagtttag ataaacaaag tacttttaga
      301 aaggagggaa aaaaatgatt tctgaacctc aaatgaataa tatgtatgaa agcccaacta
      361 taatgggagc tggtgaagct gcgccaagag cttgttcagc aattatggtg tgtgtgttat
      421 tagcatctgc aggagtaggt atccaagcag gagcggtgta tacttatgct gtagttgcta
      481 cagtagctgt agctgcagcc gcagtttgga aaacagtagg attagttggt taggaattaa
      541 taggagacta actgtctcct attgattcca atgattaagc taaggaggaa ttttaagaaa
      601 ataatgaaat acaataattt aaaaccgaaa ttacttaatg agaacataga atatataaga
      661 gaagaaaatt atggatattt aaccatagtt cccaaaaatg gaactgaatt gaaagataac
      721 gttttcaatc caacagtaag atatatattg gctaactgta ctggaaaaaa cagtgtaaaa
      781 aaaatagcga atggtttaaa aaagaagttt cctagtttag aatatgaaaa attctatcaa
      841 gatgttgtag gaactattaa atttttcaaa cac
//
LOCUS       DADXTG010000291          863 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.300, whole genome shotgun sequence.
ACCESSION   DADXTG010000291 DADXTG010000000
VERSION     DADXTG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.300"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..863
                     /locus_tag="IWI57_002836"
     rRNA            <1..863
                     /locus_tag="IWI57_002836"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          214 a          188 c          272 g          189 t
ORIGIN      
        1 atgcaggtta cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt
       61 gagtgtctgt accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc
      121 gatggaggcg ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa
      181 tcgtggtggg agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt
      241 aacggaggcg cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc
      301 agaagggagc ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga
      361 tccggtggtt ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac
      421 aggcttatct cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc
      481 gtcgcatcct ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca
      541 cgcgagctgg gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga
      601 aatttgagag gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc
      661 agttgttctg ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc
      721 atctaagtgt gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc
      781 ctgagagatg atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact
      841 gatactaatc gatcgaggac tta
//
LOCUS       DADXTG010000292          863 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.301, whole genome shotgun sequence.
ACCESSION   DADXTG010000292 DADXTG010000000
VERSION     DADXTG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.301"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>863
                     /locus_tag="IWI57_002837"
     CDS             <1..>863
                     /locus_tag="IWI57_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HBC2450909.1"
                     /translation="GEHFIKTVLVTERMSVNAFIDYCPDLVITIGKHVWSFVKYTLRD
                     KKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGVNSNNNLEYYNLWKTRL
                     SKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDFVSDDKALLSFANLGAD
                     GIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIPDNAHIFLINNHSGSEF
                     HTNFGKFYNGSLSVDKHIAAGHISKAKDWALENNIKYITADSKESLKSGLDVFINTFD
                     SPVLFEVFTDP"
BASE COUNT          275 a          115 c          172 g          301 t
ORIGIN      
        1 cggtgaacac tttattaaga ctgttctagt aacagaaaga atgagtgtaa atgcatttat
       61 tgattattgc ccagatttgg taataactat tggtaaacat gtatggtctt ttgtgaaata
      121 tactcttaga gataaaaagc ttaaatttga gcattggcgg atatctgagg atggtaagat
      181 tgttgatccg ttcaacaagc taacatctgt ctttcagtgc tcagcggaat tttttatgga
      241 gactgtcact gctggtgtga atagcaataa taatcttgag tattataatc tatggaagac
      301 aagattatca aaagtttgct atccagagtt agtctatagc aatttttatg caatttcaga
      361 actagtaaaa aaaattccag atagtgcatt agttcattct agtattttaa atgcggctag
      421 attgtatgac tttgtatctg atgataaagc attactcagt tttgctaatt taggggctga
      481 tggtattgat ggttgtttac caacattttt aggtcaggca gaagcagata aaactcgacc
      541 tgcgtattta atttcaggag acttgagttt cttttatgct atgaatagtt tatgtggtga
      601 cattccagat aatgcacata ttttccttat taacaatcat tctggaagtg aattccatac
      661 aaattttggt aagttttaca acggatcatt aagtgttgat aagcatatag ctgcaggaca
      721 tatttcaaaa gcaaaggatt gggcattaga aaataatata aaatacatta ctgcggattc
      781 taaggaaagt ctcaaatcgg ggttggatgt atttattaat acatttgaca gtcctgtttt
      841 atttgaggtc tttacagatc cgg
//
LOCUS       DADXTG010000293          860 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.302, whole genome shotgun sequence.
ACCESSION   DADXTG010000293 DADXTG010000000
VERSION     DADXTG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.302"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..711
                     /locus_tag="IWI57_002838"
     CDS             <1..711
                     /locus_tag="IWI57_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBC2450910.1"
                     /translation="AFDFQTSQQNLRRHGFVRKEEFILEEVNDNSVTFSIKPNAEMLD
                     NYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGENYEDYY
                     IEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQLKSRSV
                     TLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLEDKRQVT
                     KISPQETSRKIYDITILN"
BASE COUNT          266 a          141 c          165 g          288 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cttcgtagac atggttttgt tcgaaaagaa
       61 gaatttattc tggaagaagt caatgataat agcgtgacct ttagtattaa accaaatgct
      121 gagatgcttg ataattacct atatcagttt gaactgagag ttgtttatac cttgaatggt
      181 aaatcaatta gaaccgaatt tcaggtaacg aacttggaaa tggaaaaaac aatgccttac
      241 tttattggag cccatccagc attcaatagt cctttagtag aaggtgaaaa ctatgaggat
      301 tattatattg aatttagtga ggttgagtct tgctcgatac caaagagttt tcctgagaca
      361 ggattattag atttgcaaga tcggacacct tttttggaaa atcaaaaaat tctcgatttg
      421 gactactcac ttttttccca tgatgccatt acacttgacc agctcaagtc tcgtagcgta
      481 acgcttcggt caaggaagtc tggtaaaggt cttcgagtag attttgatga ttttccgaac
      541 ttgattctgt ggtctacaac taataaaagt ccattcattg ctttggaacc ttggagtggt
      601 ctctccactt cacttgaaga aggtaatttc ttagaagata aaagacaggt aacgaaaatt
      661 tcacctcaag aaacttctag aaaaatctat gatattacta tccttaatta aaatttgtta
      721 ttatttgagt cttcaataag atttgaatac tcttaccttg tgatgataga ttggtcttct
      781 ccgtgccagt cttacatcgc aaggttttta agttttttgt gggtctatgt caactgtagt
      841 gggtgacgaa aagctaatat
//
LOCUS       DADXTG010000294          838 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.303, whole genome shotgun sequence.
ACCESSION   DADXTG010000294 DADXTG010000000
VERSION     DADXTG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.303"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>838)
                     /locus_tag="IWI57_002839"
     CDS             complement(<1..>838)
                     /locus_tag="IWI57_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBC2450911.1"
                     /translation="NGACTDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWS
                     FAPIVDINRNWRNPIISSRSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDE
                     RDQHLSFSINSLGTEEWDNSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDED
                     LLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLF
                     FNDPDEDFNYMMEGYRKGIITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALE
                     KIG"
BASE COUNT          268 a          176 c          113 g          281 t
ORIGIN      
        1 accaattttt tccagagctt cttgcttagg taataaaatt tgttcttttg cttttttgtg
       61 taatcccaat ttagctttta accctaaaat tctttttaag gcatctaata accgatcatc
      121 agtgataatt cctttacgat agccttccat catataatta aagtcttcat ctggatcatt
      181 aaaaaataga aatagatcac atccagctgc aatagaagtg ggcaataatt ctgatcgttt
      241 cattgcccca gtcattccta acatatgact agcatcagta actactaatc cattaaatcc
      301 gagttttcct ctaagtaagt cagtgattaa ttcttttgat aaagttgcag gaagtaaatc
      361 ttcatctttc agatcaggat taaaatgttt tgaataagct ggcatatgga tatgacctgc
      421 cataattgaa ggtaatccag cttctattaa gcctgagtat acttttccaa aagaattatc
      481 ccattcttcc gtacctaaac tattaatact aaaagataga tgctgatctc gttcgtcaat
      541 accgtcacca gggaaatgtt tagctgcagg caaaattcca ctctcctgaa tacctttcat
      601 ataagctaaa ctaagttcta aaactttatc agggttttgt ccaaaagatc gactagaaat
      661 gataggattt ctccaatttc tattaatatc tacaatagga gcaaaactcc agttacatcc
      721 aatagccgat gcttcaatcc cagaaactct ccctaattca taagcatatt tcgcatcatt
      781 tgttgcacca atttttactt ctacaccaac ttcagtacca tctgtacaag ctccgtta
//
LOCUS       DADXTG010000295          825 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.304, whole genome shotgun sequence.
ACCESSION   DADXTG010000295 DADXTG010000000
VERSION     DADXTG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.304"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            145..>825
                     /locus_tag="IWI57_002840"
     CDS             145..>825
                     /locus_tag="IWI57_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBC2450912.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVD"
BASE COUNT          285 a          117 c          176 g          247 t
ORIGIN      
        1 atgaccgagc tctaggtgca gtttatttaa atggtatttg ggaagcgctt gaaaaggggg
       61 cgagtaaata attatataag ggagtttatt tagaggaaaa attttctctt ctaaaaacta
      121 acggaaggaa aggagtgttt aaatatgttt ttagataaga actttttatt agagaatgaa
      181 atagctaaaa gactttttca taaatttgca aaacaccaac caattatcga ctatcattgt
      241 catttagacc cagcatattt gctagaaaat aagcaataca agaatttgac tcaggtatgg
      301 ttaaatgata aaggagctgg agatcattat aaatggcgat tgatgcgagc taatggtata
      361 gaagaagagt tgatcagtgg tccaggtgat gattatgaaa aatttttagc ttttgtaaaa
      421 acagtggaga aggcgccagg taatcctatt tttgaatggt ctcatttgga attacaacgt
      481 tactttgata ttgatttgac aatttctgag aagaatgcac cagaaatttg gcgacgtgct
      541 aacgaaaaaa ttcaaacgga ttcctatcgc ccgaaagaat tgttggagat gatggatgtg
      601 aaagccatct gtacaacgga tgatcctaca gatgacttgt ggtatcatac tgagctttct
      661 aaacaggata attctttcaa ggtattacct actttccgtc cagataaggt ctggagtatc
      721 aaatcatcag gcttttcgaa ctatatttta aaacttagtc aaacagcggc tataccaatc
      781 aaagacttta atgggttgaa gcaagcattg agccaacgag tggat
//
LOCUS       DADXTG010000296          819 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.305, whole genome shotgun sequence.
ACCESSION   DADXTG010000296 DADXTG010000000
VERSION     DADXTG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.305"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            139..>819
                     /locus_tag="IWI57_002841"
     CDS             139..>819
                     /locus_tag="IWI57_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBC2450913.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVD"
BASE COUNT          286 a          116 c          176 g          241 t
ORIGIN      
        1 gagctctagg tgcagtttat ttaaatggta tttgggaagc gcttgaaaag ggggcgagta
       61 aataattata taatggagtc tatttagaag aaaaattttc tcttctaaaa actaagggaa
      121 ggaaaggagt gcttaaatat gtttttagat aagaactttt tattagagaa tgaaatagct
      181 aaaagacttt ttcataaatt tgcagaacac caaccaatca tcgattatca ttgtcattta
      241 gacccagcat gtttgctaga aaataagcaa tacaagaatt tgactcaggt atggttaaat
      301 gataaaggag ctggagatca ttataaatgg cgattgatgc gagctaatgg gatagaagaa
      361 gagttgatca gtggtgcagg taatgattat gaaaaatttt tagcttttgt aaaaacagtg
      421 gagaaggcgc caggtaatcc tatttttgaa tggtctcatt tggaattaca acgttacttt
      481 gatattgatt tgacaatttc tgagaagaat gcaccagaaa tttggcgacg tgccaacgaa
      541 aaaatccaaa cggattccta tcgcccaaaa gaattgctga agatgatgga tgtgaaagcc
      601 atttgtacaa cggatgatcc tacagatgac ttgcgatatc atactgagct ttctaaacag
      661 gataattctt tcaaggtatt acctactttc cgtccagata aggtctggag tatcaaatca
      721 tcaggatttt cgaactatat tttaaaactt agtcaagcag cgggtatacc aatcaaaaac
      781 tttaatgggt tgaagcaggc attgagccaa cgagtggat
//
LOCUS       DADXTG010000297          817 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.306, whole genome shotgun sequence.
ACCESSION   DADXTG010000297 DADXTG010000000
VERSION     DADXTG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.306"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>817
                     /gene="bsh"
                     /locus_tag="IWI57_002842"
     CDS             <1..>817
                     /gene="bsh"
                     /locus_tag="IWI57_002842"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBC2450914.1"
                     /translation="NDLDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADY
                     KEIQEGKDNVSPFEFIPWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLAD
                     KEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISL
                     NAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGL
                     CDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPI"
BASE COUNT          281 a          112 c          148 g          276 t
ORIGIN      
        1 aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc tgactaccct
       61 ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa tttttctggg
      121 tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt tgaatttatt
      181 ccttggattt taggacaatg ctcaacagta ggagaaacta aaaaattgtt aaaaaatatc
      241 aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca ttggctatta
      301 gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca tatatatgat
      361 aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt taatttaaac
      421 aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca aataagtttg
      481 aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc ctcagtatct
      541 cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc agagtcagaa
      601 agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg tttgtgtgat
      661 gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt tgacaaagga
      721 atctattatt atcgaacata tgaagacagt caaattactg caattgatat gaataaagaa
      781 gacttagata gtcataagtt aattagttat ccaatta
//
LOCUS       DADXTG010000298          771 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.309, whole genome shotgun sequence.
ACCESSION   DADXTG010000298 DADXTG010000000
VERSION     DADXTG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.309"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>771
                     /locus_tag="IWI57_002843"
     CDS             <1..>771
                     /locus_tag="IWI57_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HBC2450915.1"
                     /translation="IFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVNKRDGK
                     IESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDSTVIAKIAN
                     TAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMQEDSI
                     NTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELESYGIDADK
                     GLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIA"
BASE COUNT          318 a          123 c          131 g          199 t
ORIGIN      
        1 catattcaca gttgaagaat taatgaaatt gttacaatgc cgcgaaggta aagtatctaa
       61 gatcaaaaaa gaattagaaa aagcaggtct tctgaaacag aagagaggaa gagtaaataa
      121 acgtgatggt aagattgaat ctatgcctaa tttattgtac ttaggcaagc cagaagttac
      181 gaatcaagac gtctttaaaa tcatggaaga ggaagacaat actgattcca ccgtaattgc
      241 gaaaatcgca aatacggcaa aaccgcgaaa aatcaatgat tctaccgtaa ttgcgaaaat
      301 cgcaaatacg gcaaaatcca gtcatatcaa ggactcaacc gtaattgcga aaatcgcaga
      361 caatctattc tattctaatt ctatagacac taatagacac ataatagaca ctgaaactga
      421 ccagttacaa aatcaagttt tgctagataa cttcgtcgac atcatgcaag aggacagtat
      481 aaatacattt gtaccagaaa atgtattaaa cttgataaaa acattttctt caagttacag
      541 tgaagctcaa caaaccgtcc aaactattca taatgcaaag aaaaaagctg aagaattaga
      601 gcaaactatt gtcgtttatg aagaactgga atcttatgga attgatgctg acaaaggatt
      661 atatatgact ttactgaaag cttatcaaaa acaaaaaact gaaaaagtaa acaatttgca
      721 gaatctaatt ttcgtttatg taaaaaattg gtttgtcgaa aaagctattg c
//
LOCUS       DADXTG010000299          762 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.310, whole genome shotgun sequence.
ACCESSION   DADXTG010000299 DADXTG010000000
VERSION     DADXTG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.310"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..436)
                     /locus_tag="IWI57_002844"
     CDS             complement(<1..436)
                     /locus_tag="IWI57_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450916.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          231 a          133 c           96 g          302 t
ORIGIN      
        1 tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat actttataag
       61 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagaca tatttttcct
      121 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      181 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      241 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      301 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      361 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      421 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      481 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      541 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      601 ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc atttttatta
      661 ataaaatggt atattttata ttgttataaa aagagcccaa ccatcctaca ataataaaaa
      721 tttactattt gttatcttaa ccttactttt ctttctcttc tt
//
LOCUS       DADXTG010000300          733 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.311, whole genome shotgun sequence.
ACCESSION   DADXTG010000300 DADXTG010000000
VERSION     DADXTG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.311"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            200..>733
                     /locus_tag="IWI57_002845"
     CDS             200..>733
                     /locus_tag="IWI57_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450917.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNL"
BASE COUNT          306 a           82 c          135 g          210 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat cta
//
LOCUS       DADXTG010000301          731 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.312, whole genome shotgun sequence.
ACCESSION   DADXTG010000301 DADXTG010000000
VERSION     DADXTG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.312"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(118..276)
                     /locus_tag="IWI57_002846"
     CDS             complement(118..276)
                     /locus_tag="IWI57_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450918.1"
                     /translation="MDVDTSEILYDIEIDENLLDVAQEIDHQKQEAIRYAGRKGKRKY
                     LLRECSKS"
     gene            complement(267..605)
                     /locus_tag="IWI57_002847"
     CDS             complement(267..605)
                     /locus_tag="IWI57_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450919.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          217 a          149 c           88 g          277 t
ORIGIN      
        1 ctttctttag tccaaaaaac agttttttct atcgatcctt ctctgtaaac tgtaaatgtc
       61 ggagtcgagt taattgagta gcgactcttg aattctttcc actcattttc atcttctcta
      121 acttttgcta cattcacgta ataaatattt tcttttccct tttctaccag catatcgaat
      181 agcttcctgc ttttgatgat cgatttcttg tgctacatct agtaaatttt catcaatttc
      241 tatatcataa agtatttcac tagtgtctac atccacaata tatacttttt atcagtagca
      301 atttttgagt aatccgttaa aatgatctca gtctcgtcac tttcatcagt ataaatatac
      361 gttaacaatg aactcacctt ttcaatcggt gttagctcta agcctactgt attttttact
      421 attgagtcat tatttaccac cataacttcg tactgtatat accatcctat ttggtttcca
      481 tcaatatcag taattggctc actagctaat ggagtctccg gaacaatatt ttcctgatat
      541 ttcttaaact cttctgtagc acgaaaaagg ttatcatgtt cctcacttga aactggttga
      601 gtcatagaaa atgttacttg gctaaacaga gggaaaataa acactacaat caacaaagta
      661 taaaaaaaag cccttctttt tcatttttta tcttccaatc acaaattgaa aaacttattc
      721 ctgcaacttt g
//
LOCUS       DADXTG010000302          718 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.313, whole genome shotgun sequence.
ACCESSION   DADXTG010000302 DADXTG010000000
VERSION     DADXTG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.313"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            270..343
                     /locus_tag="IWI57_002848"
     tRNA            270..343
                     /locus_tag="IWI57_002848"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Asn,seq:gtt)
     gene            complement(481..645)
                     /locus_tag="IWI57_002849"
     CDS             complement(481..645)
                     /locus_tag="IWI57_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450920.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
BASE COUNT          249 a          129 c          111 g          229 t
ORIGIN      
        1 gaaccggcga cctcttcctt accatggaag cgctctacca gctgagctaa agcggcatta
       61 tcattaatac aaataatagg ataacgaata ttaatgtatt tgtcaacaga attttttcga
      121 aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa aataaaaata acaaaaaatg
      181 cgaacggttt ctttgataat ataataaaaa aattcaaaaa aagtattgcg ttatttagaa
      241 gaaattgata ttatattcat gtacttgatt ccggcatagc tcagttggta gtagcgcatg
      301 actgttaatc atgatgtcgt aggttcgagt cctactgccg gagtaaataa agcttatgca
      361 aagaaaaaga aatccaatca cttgggtttc tttttctttt attattatga tgaatcaagc
      421 tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga gtagagttgg ctactcataa
      481 tcaaccatac ttcccatcat cgctaaatac gaaataaatc agcattccta caccaatcaa
      541 agcacaagct gcaccaagaa aagggatgac attcccaaag ttatctgtat tcataagtat
      601 gccaccaccc acaaacaaaa agccgccagc aatcaggttt ttcattttat tcatctcctt
      661 tcttacgtta tagtatttcc ttctttcaat gagtattttg aaagctatct tattgtat
//
LOCUS       DADXTG010000303          710 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.314, whole genome shotgun sequence.
ACCESSION   DADXTG010000303 DADXTG010000000
VERSION     DADXTG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.314"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>710)
                     /locus_tag="IWI57_002850"
     CDS             complement(<1..>710)
                     /locus_tag="IWI57_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450921.1"
                     /translation="SNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDS
                     GREYELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKS
                     SKNLRCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETF
                     NWLLENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVN
                     FFDDSKESVTCILSGEQL"
BASE COUNT          238 a          138 c           89 g          245 t
ORIGIN      
        1 ctaattgttc ccccgacaaa atacaagtca cactttcctt actatcatca aaaaaattaa
       61 catcagaaag caagacatca tagacacgtc gcatttcctc tagataattc cttattgtcc
      121 tgaaaagttt gtctttcatt gattcaagag tgtcaaaaag tacttttgat tcctctttca
      181 attttcgaaa atgtgtagga tacaagtcta ggttctctaa tagccaatta aatgtttcta
      241 cccgactgtc tttctttaaa attagcatca caactccaat aaaattttca ctgatatcac
      301 aattactgga atgacaataa aaaatggaat gcccagtttt actggtaaaa atactagcag
      361 agggattatg atcgttgtga aacagacatc ttaagttttt acttgactta agtaatttat
      421 tcatatcaat ggttaacaat ctttcataaa tctcattaaa agacatgtcg gaggaatcaa
      481 acaggcagtc tttaaaccac tgtttcattt cttctttgtc gtcatttttt atcaacttaa
      541 caaaatcggt ttcattgact agttcatact ctctccctga atcactttga caatttttag
      601 aacatgtcat ggtaaaatca atcggaagct cagtaatatc taacatgttg tcttcattaa
      661 ttattatcac tgcatctgac ttatatcctc caaaaaacat tctattcgaa
//
LOCUS       DADXTG010000304          704 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.315, whole genome shotgun sequence.
ACCESSION   DADXTG010000304 DADXTG010000000
VERSION     DADXTG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.315"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..268
                     /locus_tag="IWI57_002851"
     CDS             <1..268
                     /locus_tag="IWI57_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBC2450922.1"
                     /translation="KIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVK
                     EMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            269..>704
                     /locus_tag="IWI57_002852"
     CDS             269..>704
                     /locus_tag="IWI57_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBC2450923.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT          271 a           78 c          126 g          229 t
ORIGIN      
        1 taaaattata gaaattcatt tagattattt tggaagagtt cctacaagaa aatttcaatt
       61 gtttacttta gatgaatgta tcaatgttga cttattaaat aataaaataa gttttgaaga
      121 aaacattgtt aaagaaatgc ctgaatctgc taatgatatg tatgagaagg agttgaaata
      181 tttctttgaa aacgtagtaa caggcaaaga aaactggaat gatttatatc atgcaaacga
      241 agtattgaaa atagctgagg agaaataaat ggaaatttta tttactattt gtggtagagc
      301 tgggtctaag gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc ccctagtcca
      361 tttcgcttta gcaatgatag atatctatca acaaaaacat cctgaacata attgcacgac
      421 ttgtttgaat acggacagta atgagttagt agaattgacg actaagttaa atccgtctgt
      481 gaaaattgta gaacgtaaac ctgaattagc tacagatact gtagggaaaa ttgaagtgat
      541 acgtgattca ttcttacaac tagaaagaga agataatata tttgatttaa ttatagattt
      601 agatttgact tcacctttac gtgaagtttc taatcttgaa gaattaatag atgaacatct
      661 aaaaaattta gataaagacg tctacttttc agttgtttct gcaa
//
LOCUS       DADXTG010000305          694 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.316, whole genome shotgun sequence.
ACCESSION   DADXTG010000305 DADXTG010000000
VERSION     DADXTG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.316"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..632
                     /locus_tag="IWI57_002853"
     CDS             18..632
                     /locus_tag="IWI57_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2450924.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT          244 a          128 c          137 g          185 t
ORIGIN      
        1 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
       61 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
      121 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
      181 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
      241 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
      301 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
      361 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
      421 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
      481 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
      541 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
      601 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
      661 atctttaatt tcctacatat aattactatc tata
//
LOCUS       DADXTG010000306          684 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.317, whole genome shotgun sequence.
ACCESSION   DADXTG010000306 DADXTG010000000
VERSION     DADXTG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.317"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..173
                     /locus_tag="IWI57_002854"
     CDS             33..173
                     /locus_tag="IWI57_002854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450925.1"
                     /translation="MILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAET
                     HA"
BASE COUNT          240 a          108 c          135 g          201 t
ORIGIN      
        1 aattagcttc aaaaaaaacg aatagttttg gattgatatt aagcgagtat aaaatcaaag
       61 cagaagagag ttatttctat caattactaa aacggtggag taagcttccc gccgattttt
      121 atcgaaaaat gcagatcatc aactatcaac cgcttgctga gacacatgcc taaatagagg
      181 ttgtgaaatc agtgctttga gctaataccg aaggtcagct gattgaacat tgtttagaag
      241 agattgtgac aactgcgatt tgctttgagt aataagaacg ctggacagaa aatagcttct
      301 catattttct agtctagtgg gcttattgcc gagaagcagc agatggaaca tcgtttagaa
      361 gagattgtga caactgcgat ttgttttgag taataagaac gctggacaga aaatagcttc
      421 tcatattttc tagtctagtg gacttattgc cgagaagcag cagatggaac atcgtttaaa
      481 gaagagatcg gtacaaaata aaaatatcta aatttaagtt ttatctaagt ttcgctttgt
      541 acctttgatt caaatcaaag gaggcgatct tgatgctgaa aaaatggatc acactatttt
      601 ttgccactcc ccattctaga atcaacccgt atgatgtaga taatgaattc acgttgaaaa
      661 aagatttctc aaataaataa aaac
//
LOCUS       DADXTG010000307          662 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.318, whole genome shotgun sequence.
ACCESSION   DADXTG010000307 DADXTG010000000
VERSION     DADXTG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.318"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>662
                     /locus_tag="IWI57_002855"
     CDS             <1..>662
                     /locus_tag="IWI57_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBC2450926.1"
                     /translation="IIKEALNLDCNIFTEINLLNEYYDEIIEVAKKKDRLLYLSSTFL
                     KRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIEL
                     PWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNL
                     TIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKE
                     FFT"
BASE COUNT          248 a           74 c          112 g          228 t
ORIGIN      
        1 attataaaag aggctttaaa tttagattgt aatatcttta cggaaatcaa tttattaaat
       61 gaatattatg atgaaataat cgaagtagct aaaaagaaag atagattact ttatctatct
      121 tctacatttt tgaagagaaa agaaattcaa tatattaagt ctaaagtgac gcataataaa
      181 aaaatttctt atcaatacca tgtaggacag tatttacccg actggcatcc ttgggaagat
      241 tatcgcaatt tttttgtttc aaacaaaaaa actaatgggt gtagagaaat attcgctatt
      301 gaattacctt ggattataga tacatttggg aaagttaaat cgtttactgt aatgacccag
      361 aaattatcca atcttgatat tgattattcg gacagtttca ctcttattat tgagcatgaa
      421 actggtatag ttggaacttt aagtgtagat gttgtttcaa gagtagctaa aagaaattta
      481 actataattg gtgaaaagat tttaatagaa tgggaaggta acccggattc tctattttca
      541 tggaatgaag aaacttctag tatgacagat attcaattgt acgatgaggt tatgcatgat
      601 aataattatt cacatactat tattgaagat gcatatttag aggagattaa agaatttttc
      661 ac
//
LOCUS       DADXTG010000308          660 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.319, whole genome shotgun sequence.
ACCESSION   DADXTG010000308 DADXTG010000000
VERSION     DADXTG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.319"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>660
                     /locus_tag="IWI57_002856"
     CDS             <1..>660
                     /locus_tag="IWI57_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBC2450927.1"
                     /translation="KGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQ
                     QELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMG
                     TQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSC
                     VRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFG
                     ET"
BASE COUNT          248 a           88 c          130 g          194 t
ORIGIN      
        1 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
       61 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
      121 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
      181 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
      241 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
      301 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
      361 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
      421 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
      481 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
      541 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
      601 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
//
LOCUS       DADXTG010000309          645 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.320, whole genome shotgun sequence.
ACCESSION   DADXTG010000309 DADXTG010000000
VERSION     DADXTG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.320"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>645
                     /locus_tag="IWI57_002857"
     CDS             <1..>645
                     /locus_tag="IWI57_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBC2450928.1"
                     /translation="DLQEELALEPMVEKESYPISVPSSYNDLFETENIRNHVGWVWYE
                     KEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQHKGGFTPFESEINQFIKDGSNR
                     ITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVNFDFFNYAGINRPVKLYTTPKDY
                     IKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDEEAQLIAEAKGKEGVIQLK"
BASE COUNT          256 a           78 c          125 g          186 t
ORIGIN      
        1 tgacttacaa gaagaacttg ctttagaacc aatggttgaa aaagagagtt atccaatttc
       61 agttccttct tcttataatg atttatttga aacagagaac attagaaatc atgttggatg
      121 ggtgtggtat gaaaaagaag tgattcttcc taaaaaatta ttagaagaaa gattagttct
      181 tcgttttgga tctgcaacac atgaagcaaa agtatttttg aatggaaaat tgattaatca
      241 acacaaagga gggtttacgc catttgaatc agaaattaat cagtttataa aagatggaag
      301 taacagaatt acagtggctg taaacaatgt tattgacgat agcactttac ctgtagggat
      361 tttgaaagaa acgatacagg aagatggaga cataaaaaaa gaaaatttgg ttaattttga
      421 ctttttcaat tatgcaggca ttaatagacc cgttaaattg tatacaactc cgaaagacta
      481 tataaaggat gtcactatta atacaaaaat aattgattca gatgcaaatg tagaatttaa
      541 tatcgataca gaatggaccg gagaaattct aatagcaatc tatgacgaag aagctcaact
      601 tatagcagaa gctaagggga aagaaggagt gattcaactt aagaa
//
LOCUS       DADXTG010000310          632 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.321, whole genome shotgun sequence.
ACCESSION   DADXTG010000310 DADXTG010000000
VERSION     DADXTG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.321"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..613
                     /locus_tag="IWI57_002858"
     CDS             <1..613
                     /locus_tag="IWI57_002858"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBC2450929.1"
                     /translation="AGVIAVVRGKNKDEAIKAINSIIAGGIKGIELTFTVPNAEEIIK
                     EIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTAEICNLYQIPYLP
                     GCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMPTGGVSLENLEDW
                     FEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
BASE COUNT          212 a           85 c          143 g          192 t
ORIGIN      
        1 ggctggagtc attgcggttg tacgtgggaa aaataaggat gaggccatta aagcaataaa
       61 ttcaattatt gcgggtggaa ttaaagggat tgaactaact tttacagttc ctaatgctga
      121 agaaataatc aaagaaattg ctaaaaaata tgctgatcaa aaagatgtaa taatcggagc
      181 cggtacggtt ttagatgcgt ttactgctcg tatcgcaatt atggctgggg cagaatatat
      241 cgttagtcca tcttttgatc gacaaacagc tgagatttgt aatttatatc aaattcctta
      301 tttaccaggt tgtatgacaa ttactgagat aaaggaggca cttactgctg gcgtagatat
      361 cattaaacta ttcccaggtt ctgcttttgg tccatccatt gtaaaagcct atcaagctcc
      421 attaccacac gtaaatataa tgcctactgg cggagtaagt ctagagaact tagaagattg
      481 gtttgaagta ggagttgttg cagttggtgt aggtgggaat ttattggctt ctgttgaaaa
      541 aggtgatttt gaagaagtaa gtcgagtagc tagtaaatat atagataagt tttatgagat
      601 aaagaattat taggagaaaa aacaaataat ac
//
LOCUS       DADXTG010000311          625 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.322, whole genome shotgun sequence.
ACCESSION   DADXTG010000311 DADXTG010000000
VERSION     DADXTG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.322"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..>625
                     /locus_tag="IWI57_002859"
     CDS             29..>625
                     /locus_tag="IWI57_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBC2450930.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTS"
BASE COUNT          244 a           95 c          111 g          175 t
ORIGIN      
        1 aaaaatgaaa ttttgagagg gattaaatat gacaattaaa actagtctcc aaaatcaagc
       61 ttatcaatct attcgtcagc aaattatcta cgctgactta gaacctggaa aaaaagtatc
      121 agaaaaaaaa ttggaagaaa cgctttctat cggtcgtact ccaatacgtg aagcattgat
      181 tcaactccgt catcaaggac ttgttgatac aattcctcaa agtggaaatt atatctcatt
      241 aatcgactta gatttagcaa aaaatgctcg ttttgtcaga gaacatttag aacgacaaat
      301 tatgctagag tgttgtgcca agatgaataa tcaaaagaaa caagttttag aaactataat
      361 tgcagaacaa gaaaaagctg ttagtcaaaa gaatgctcgt agtttttttc ataatgataa
      421 tttatttcat aaagcttgtt ttgaaattgc agaacgggaa gaagtatggc aatggttgga
      481 agataataat acgcacttgg aacgatttcg ttggttacga gtaaaaacgg aaggattaaa
      541 atgggaaaaa attatggaac aacatcatca actatttaaa gccttgacca tgcaagaccc
      601 tgaagaagct aacttcttaa catcc
//
LOCUS       DADXTG010000312          619 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.323, whole genome shotgun sequence.
ACCESSION   DADXTG010000312 DADXTG010000000
VERSION     DADXTG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.323"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IWI57_002860"
     CDS             complement(<1..430)
                     /locus_tag="IWI57_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBC2450931.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          194 a          124 c           89 g          212 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tgaatcggct acactagact
      481 agacagaaaa aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgcca
      541 agacgtcaac gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcga tctctatctc
      601 gctggtaagc ctcgcgcag
//
LOCUS       DADXTG010000313          616 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.324, whole genome shotgun sequence.
ACCESSION   DADXTG010000313 DADXTG010000000
VERSION     DADXTG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.324"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            95..565
                     /locus_tag="IWI57_002861"
     CDS             95..565
                     /locus_tag="IWI57_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBC2450932.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          222 a          108 c           93 g          193 t
ORIGIN      
        1 ttattgtctg aaaagagttc taaacgggaa aacaaatagc tagttttcag tttagcgctc
       61 tttttattat ataaaataga aaggagaaaa ctttatgtct atttataatt ttgaaactac
      121 tttagaagac ggcactgtct attcattgaa aaaatatgca ggcaaaccga taatcattgt
      181 taacactgca acaaaatgcg gtctagcacc tcaatttaaa caattagaag aactttacca
      241 agcttatcag acacaaggtc tggttatctt aggctttcct tccaatcaat ttaaacaaga
      301 gctagattca gcgactgaag cagcagaagc ttgtaaaacc acttatggcg ttaccttccc
      361 tatgcatcaa ttgattgacg taaatggaga aaatacagat cctcttttta cttatcttaa
      421 aaatgaagct cctggtactg tcactaaagc aataaaatgg aattttacta aatttttgat
      481 tgatagaaat ggcaaagtaa tcgaacgatt tgcaccacaa acttctccat tgaaaatgac
      541 cgctgcaatt gaatccgttc tttaacttaa taaaaataaa aaaagatgaa ctatttctag
      601 ttcatctttt tgcctt
//
LOCUS       DADXTG010000314          612 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.325, whole genome shotgun sequence.
ACCESSION   DADXTG010000314 DADXTG010000000
VERSION     DADXTG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.325"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>612
                     /locus_tag="IWI57_002862"
     CDS             <1..>612
                     /locus_tag="IWI57_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HBC2450933.1"
                     /translation="LETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSF
                     DDDLSETIDKSTSSRNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTET
                     DQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKAE
                     ELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQ"
BASE COUNT          262 a          101 c           99 g          150 t
ORIGIN      
        1 agttagaaac atataatttg atttcagtcg taaaaggaga atttaaccca aaaacgaaaa
       61 aaaatgaaaa aaataaatac tatttacttc aacctgaaat aagtgccgat gatcttttta
      121 ttcaaaattc tttcgatgac gatttaagcg aaactattga taaatcaacc tcttctcgga
      181 atgcggaaat cgcacgtcga gaaaaaacag ctgaaaccct tgataacact ggaaatgcgg
      241 aaatcgcaca agattattat attaataaat ctttagacac taaaagacac ttaaaagaca
      301 ctgaaacaga ccaattacaa aatcaagttt tgctagataa cttcgttgaa atcatgaaag
      361 aagacagcat aaacacattt gtaccagaaa aggtattaag cttgataaaa acattttcta
      421 acacttacga agaagcccaa caaaccgtca ccacaataca caacgcaaaa cataaagccg
      481 aggaacttga gaagacggta gttgtgtatg aagaacttga atcttacgga attgatgcgg
      541 ataaaggatt atatatgacg ttgctgaaag cttatcaaaa acaaaagact gagaaagtaa
      601 ataatttgca ga
//
LOCUS       DADXTG010000315          597 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.327, whole genome shotgun sequence.
ACCESSION   DADXTG010000315 DADXTG010000000
VERSION     DADXTG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.327"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(233..382)
                     /locus_tag="IWI57_002863"
     CDS             complement(233..382)
                     /locus_tag="IWI57_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450934.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          188 a           94 c           92 g          223 t
ORIGIN      
        1 attatacaga taataacgga atattatcat cgaaacagct aacaaaattg tcataaaaaa
       61 cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa gagagacaat
      121 ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt tacaaacgtc
      181 ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact ccttatctag
      241 attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg ttattttcgt
      301 tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg cggtattgca
      361 tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt agttcttgaa
      421 agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt ttgatgcaat
      481 taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc tattttcttt
      541 taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa tataaat
//
LOCUS       DADXTG010000316          585 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.328, whole genome shotgun sequence.
ACCESSION   DADXTG010000316 DADXTG010000000
VERSION     DADXTG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.328"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(84..>585)
                     /locus_tag="IWI57_002864"
     CDS             complement(84..>585)
                     /locus_tag="IWI57_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBC2450935.1"
                     /translation="GLSTFVSTGTSVYATEKVNTVDLEIKNKLVDELTFYFDTVGFLD
                     EHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKVDWKRYAGCVAINSLPG
                     GGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAAKELAKVNIVAGIAAAA
                     ASCALE"
BASE COUNT          178 a          129 c           65 g          213 t
ORIGIN      
        1 tagttatggt ttaaatagat attttcttta aacattgctt gtttaaaagc catatgctct
       61 tgttctaaac ttttcataaa cttttactct aatgcacagc tagcagctgc tgctgcaatt
      121 cctgctacaa tatttacctt agctaactct ttagctgcag cttttcctaa ttgtttttct
      181 gcaattttta ataataaatc cgcagctttt tcaaaattaa aagcttttaa tgcttcaata
      241 aaatctccct gcgtacctat agcccaagcc actcctcctc caggaagaga attaatggct
      301 acacatccag catatcgttt ccaatcaact ttctccattc taggaacaat aatatgttca
      361 taaaagactc ttgctgctct tccttgttcg ttatcttgac tgattcgttc acgaatagca
      421 ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa aaccaactgt atcaaaataa
      481 aaagttaatt catctactaa tttattcttt atttctaaat caactgtatt cactttttct
      541 gtggcataca cacttgttcc agtactaaca aaagtactta aacct
//
LOCUS       DADXTG010000317          570 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.329, whole genome shotgun sequence.
ACCESSION   DADXTG010000317 DADXTG010000000
VERSION     DADXTG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.329"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(4..198)
                     /locus_tag="IWI57_002865"
     CDS             complement(4..198)
                     /locus_tag="IWI57_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450936.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(298..384)
                     /locus_tag="IWI57_002866"
                     /pseudo
     CDS             complement(298..384)
                     /locus_tag="IWI57_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          200 a          104 c           84 g          182 t
ORIGIN      
        1 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
       61 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      121 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      181 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      241 ttgttactca taattgtacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      301 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      361 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      421 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
      481 atttaagtat tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
      541 acgattattt gtaacaaaat gaatattata
//
LOCUS       DADXTG010000318          542 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.330, whole genome shotgun sequence.
ACCESSION   DADXTG010000318 DADXTG010000000
VERSION     DADXTG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.330"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>542)
                     /locus_tag="IWI57_002867"
     CDS             complement(<1..>542)
                     /locus_tag="IWI57_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450937.1"
                     /translation="MGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSIIY
                     TFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHL
                     LSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQEL
                     KVPLGKELSGMAPSDMLTHI"
BASE COUNT          218 a           91 c           88 g          145 t
ORIGIN      
        1 aaatatgtgt cagcatatca ctaggagcca taccacttaa ttcttttccc agtgggactt
       61 tcaattcttg tgctctagat tgttgaataa aaggaattaa gaatcccgca cttaaaaaaa
      121 aggtgaggac ggtagctttt aaaagactta agagtctagc gattttttgg tcccaaaaat
      181 aaaaagaaag aaacaacgtt cctccaataa atacagaaac catagcgaca gaaagtaaat
      241 gagtatacac tactagcgtc atgccaatag acaaccagta ccatttttgg taatctctaa
      301 tataaatttc gtaacacccc attagtatca aaggcaaaaa tgttaaagcg acagcttctc
      361 ccaatgaagc acgatggaat atgtcaatgg cacgataggc ggaaaatgta taaataatcg
      421 aaaataacag cgaaatataa cgattgtttt ttatttgttt catagaaaaa taagaaacga
      481 ccattgttaa aaaagtgata aaaaaatagt agatattata acttaaaaca agatttccca
      541 tc
//
LOCUS       DADXTG010000319          538 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.331, whole genome shotgun sequence.
ACCESSION   DADXTG010000319 DADXTG010000000
VERSION     DADXTG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.331"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            194..406
                     /locus_tag="IWI57_002868"
     CDS             194..406
                     /locus_tag="IWI57_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450938.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT          159 a          103 c          107 g          169 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaaatcta attagctttt ttagtcaatc taaagttctt tattctatga tataatgtta
      181 ctgtagaaag tttgtggcgg tttggcaaat ccaattgaag gagcgtggtg cctatgggct
      241 tattagctta tcggacgtac tctgtagaaa ggaaatgcct gatttgttct cattggatca
      301 aatcattgct attatcggag cagcagcagt agttattact gccgtagcga aagctagcga
      361 ttcgatttta gcttggttaa agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt
      421 ttggcgaggt agacgagctc taaagcttta tttacatgcc atccgctttg aacggactac
      481 actgggcatg tgacagcatg ccctttcatt tatattatat ctcattcttt tatataat
//
LOCUS       DADXTG010000320          538 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.332, whole genome shotgun sequence.
ACCESSION   DADXTG010000320 DADXTG010000000
VERSION     DADXTG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.332"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(7..>538)
                     /locus_tag="IWI57_002869"
     CDS             complement(7..>538)
                     /locus_tag="IWI57_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBC2450939.1"
                     /translation="IESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRP
                     TLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFIC
                     IYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVF
                     WYYLGSISYLYLKKEN"
BASE COUNT          198 a           97 c           65 g          178 t
ORIGIN      
        1 ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat aataccagaa
       61 aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca tttcatctat
      121 aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca ctttacttct
      181 aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga tacatataaa
      241 agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt ttccatgtgc
      301 tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa attttgcatc
      361 gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc ttataacttt
      421 aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat cactagaatg
      481 ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg actctata
//
LOCUS       DADXTG010000321          527 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.333, whole genome shotgun sequence.
ACCESSION   DADXTG010000321 DADXTG010000000
VERSION     DADXTG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.333"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(104..301)
                     /locus_tag="IWI57_002870"
     CDS             complement(104..301)
                     /locus_tag="IWI57_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBC2450940.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          176 a           97 c           74 g          180 t
ORIGIN      
        1 attaatgaac gaaaaaaatc cctcacctaa tattacggtg aggaattttt tctaccgaca
       61 taaacaaaaa aaccacctca tatgaggcgg ttaacgttca acattatttt acgatattta
      121 ctgcttgagg accacgttgt ccttcttcaa tatcataagt aactgcttgg ccttcatcta
      181 aagacttaaa gccttctcct tggattgctg agaaatgagc gaatacgtca tttccatctt
      241 caccagtgat gaaaccaaaa cctttatctg agttaaacca ttttactgta ccgttattca
      301 tatatatttc ctcctgatac gtatataata cgtgttttat tgcaattaat attacttggt
      361 aaaaggagtt tttatagtgt gaatatatcg ctcagattac gtttcaaatt agattactta
      421 tttactataa cacggatgta atgctaacac aaggataatc actcgatata cggtttaaat
      481 attattattt gtcaaattaa gcgagacaaa actttccatt tacgtaa
//
LOCUS       DADXTG010000322          524 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.334, whole genome shotgun sequence.
ACCESSION   DADXTG010000322 DADXTG010000000
VERSION     DADXTG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.334"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>524
                     /locus_tag="IWI57_002871"
     CDS             <1..>524
                     /locus_tag="IWI57_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBC2450941.1"
                     /translation="WNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMAN
                     KIAQGEVVSGKTPLGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGI
                     VRDYQSVKNMLTNKKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFK
                     GLLKCSDCQGSVAG"
BASE COUNT          194 a           72 c           91 g          167 t
ORIGIN      
        1 ttggaatgct gtttctcaac aatattatga tacaactaca gcgtatggca gaactttcat
       61 tgcacaagta atgagttttg ctgaattgga agcccaaatt gattcagaac gcatcaaagc
      121 tgttatggca aataaaattg cacaaggtga agtagtaagc gggaaaactc cgttaggcta
      181 ctctatagaa aataagaaat tagtaataaa tgatgatgct cctatcgtga tagatatatt
      241 caactacttt ctttctagtg gtagtttaag aaaaactgtt tattatttag gctctcagta
      301 tggaatcgtt agagattatc aaagtgttaa aaatatgttg acgaacaaaa aatacattgg
      361 tgaattaaga aataataaaa attactgtcc tcctattatt gataaaaaac ttttttatgc
      421 tgttcaaaaa gccttaccta aaaatttaaa aacaaatgca aaacgtgatt acatttttaa
      481 aggtttatta aaatgttctg actgtcaggg atcagtagct ggtc
//
LOCUS       DADXTG010000323          521 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.335, whole genome shotgun sequence.
ACCESSION   DADXTG010000323 DADXTG010000000
VERSION     DADXTG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.335"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            164..463
                     /locus_tag="IWI57_002872"
     CDS             164..463
                     /locus_tag="IWI57_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBC2450942.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          199 a           61 c          101 g          160 t
ORIGIN      
        1 tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac agtgtattga
       61 aaggttttaa caatccagta atgatttaaa aaaatctaga tgtagcgtat actttgttta
      121 tagaatgtag tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta aaagtgaaga
      181 aatagaaaac atggtactaa aattatatga tctcgtttta aatccagaga tcaaagaaaa
      241 ggaacgtata cttttgatag acgcaaagac aggcttagaa aaaggtcagt attatcctaa
      301 agtcataaat aatttggaac gtagtttacg tccgttagca atcaggggag aattgtctaa
      361 accagtatca ccgttttata tggagatatc aacaatagga aaatttgaaa aagaattagg
      421 tcgaggaatg gcttcggcac cgattacttt tgggcattta taaaaacgag ttattcatct
      481 caatgaatta agaagtatag tcaatctcat aatgcaggct a
//
LOCUS       DADXTG010000324          520 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.336, whole genome shotgun sequence.
ACCESSION   DADXTG010000324 DADXTG010000000
VERSION     DADXTG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.336"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>520
                     /locus_tag="IWI57_002873"
     CDS             <1..>520
                     /locus_tag="IWI57_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBC2450943.1"
                     /translation="LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRS
                     RFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKV
                     GYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKG
                     YISHKIHEKLAL"
BASE COUNT          173 a           97 c           93 g          157 t
ORIGIN      
        1 aactgaagca acgcgataca gtgattattg cttgtgttat atggggcata attaatggct
       61 atactagcca aagagccacg tatagagcgg tttgttccgt cttatttcct aatggtgact
      121 ttcctagtag gagtcgattc actcgcttaa gttcaaactt agcttacact atcaagatta
      181 ttcgatactt tttcatcaag aagctcacca aaggtgaact agtcggaatt atagatagtt
      241 ttcctagtcc attatgtaaa ccagttagaa atagacaagc aaaactattg aatcaaatag
      301 ccaaagttgg ctataactca acaaaaaaat cttattttta tggtcttaaa attcatatga
      361 tcgttactaa gactggcttt ccgattactt actcaatcac gaatccaggt gtccatgacg
      421 tgaaagtgtt agagacttta tcggaagaag cgaatcttcc caatatccta ggggacaaag
      481 gatatattag ccataaaatc catgaaaagc ttgcccttaa
//
LOCUS       DADXTG010000325          520 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.337, whole genome shotgun sequence.
ACCESSION   DADXTG010000325 DADXTG010000000
VERSION     DADXTG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.337"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(64..414)
                     /locus_tag="IWI57_002874"
                     /pseudo
     CDS             complement(64..414)
                     /locus_tag="IWI57_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          151 a          109 c           61 g          199 t
ORIGIN      
        1 agccataaca ccgcagcatt cacaacacct tataattttt gtcaaaaaaa gtgttgccat
       61 tccatctggc attaagaaag gatatgaagc ctttgtcttt tcatgtatta actttgactt
      121 ttcaattgct tccttgtttt caccgttatc atcaacagga tctgtaacaa ctccaacgat
      181 atttacaccc ttgcttttca tttcattctg aacctcaacc aaatcaggaa tttcctttac
      241 acaagctgta caccaagttg caaatacatt taccatagta agatcatact tctcaaaatc
      301 tttacttgta aaatccttgc catttatgtc ctttgttggt agtttacgta aatctttaac
      361 cgattctttg ttaaatgctt ctgtattttc taaatcagtt ttttctgata gcacaaaacc
      421 atttttagga cgttcttttt tatctataat ttgaatctca gttcttttta attcatcaag
      481 aagattactt tcagctccgc tatttgtgct taaatagcaa
//
LOCUS       DADXTG010000326          520 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.338, whole genome shotgun sequence.
ACCESSION   DADXTG010000326 DADXTG010000000
VERSION     DADXTG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.338"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            105..299
                     /locus_tag="IWI57_002875"
     CDS             105..299
                     /locus_tag="IWI57_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450944.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          219 a           67 c           86 g          148 t
ORIGIN      
        1 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
       61 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      121 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      181 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      241 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      301 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      361 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      421 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      481 caaatatact tgtcattcat catgaaatat agagacttat
//
LOCUS       DADXTG010000327          519 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.339, whole genome shotgun sequence.
ACCESSION   DADXTG010000327 DADXTG010000000
VERSION     DADXTG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.339"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>519
                     /locus_tag="IWI57_002876"
     CDS             2..>519
                     /locus_tag="IWI57_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBC2450945.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQT"
BASE COUNT          190 a           96 c           83 g          150 t
ORIGIN      
        1 tatgaatgat tttactacag aaattgtgca aactctagtc actaaaggcg atttaaatga
       61 attattccgt tcgcacttag aaaaagcgat aaacacactc ctacggactg aattaacggc
      121 ttttttagat tacgaaaaat atgatcgcac tggttttaat tcaggtaatt cgagaaacgg
      181 ttcttacttt cgatcaatca aaaccgaata tggtgaatta acattggaaa tacctagaga
      241 tcgtaatggt gagtttaaac aacaaacttt accagcctac aaaagaacaa acgatacatt
      301 ggaaaccact attatccatt tattcgaaaa aggtgttacg atgtctgaaa ttgctgattt
      361 gatcgaaaaa atgtacggtc atcactatac tccacaaacc atgtccaaca tgactaaagt
      421 tctgactgaa gaagtaaatg cctttaaatc cagagcctta aatgataagt atgtcgctat
      481 ttttatggac gctacttaca ttccactaaa acgtcaaac
//
LOCUS       DADXTG010000328          514 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.340, whole genome shotgun sequence.
ACCESSION   DADXTG010000328 DADXTG010000000
VERSION     DADXTG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.340"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWI57_002877"
     CDS             complement(<1..132)
                     /locus_tag="IWI57_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBC2450946.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DADXTG010000329          508 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.341, whole genome shotgun sequence.
ACCESSION   DADXTG010000329 DADXTG010000000
VERSION     DADXTG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.341"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(140..505)
                     /locus_tag="IWI57_002878"
     CDS             complement(140..505)
                     /locus_tag="IWI57_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450947.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          205 a           66 c          103 g          134 t
ORIGIN      
        1 tacacaactg tttttagtgt ttggagagaa gaaaagaagt aaaaaataga aaaactagtg
       61 atagttttgg tgatataaga aaaacatata tgtataaaaa gaagaaagta gcttctcttt
      121 gtacatatat ggtttgtttt tatggatgcc gagctttttt ataagataag gctaccgccc
      181 ataacgaccc aaggatttgt ggaactaagt aaatacctgc aagaagcaag atcagctggg
      241 tcggttttaa aggatgatag aagtaacgcc attcagtaaa attggcgcaa aataaatagg
      301 aagtgacagt agatataata ttggcaatga ataaagatat aatacgcctg ttcctttgaa
      361 aataatatcc agcaaaaaga gataaaaata tcaatgtgat gaacccgctt acggaatgat
      421 aacgaaaatc aagcagtaag gcgaaatgca cataaggaac aaaagataaa agcaacggaa
      481 ggtttttcca gcaagttttt atcatctt
//
LOCUS       DADXTG010000330          508 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.342, whole genome shotgun sequence.
ACCESSION   DADXTG010000330 DADXTG010000000
VERSION     DADXTG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.342"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            245..>508
                     /locus_tag="IWI57_002879"
     CDS             245..>508
                     /locus_tag="IWI57_002879"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBC2450948.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          166 a           98 c           78 g          166 t
ORIGIN      
        1 tattatttcg agaaagataa atattttcaa aaaccttttc ataatcctca ccctttctta
       61 cactgataaa tcatgcgtct aatattcaaa ttatactcga tatgctttag ctaatcagcc
      121 aactctgttt gtctactatc attatatcaa tagacaatat ctgctcccgt ctttatgatc
      181 gtgccaaagc aatcaaaaga aaattgcgaa tcacttagat cgaaagttta ctcaaacaaa
      241 agcattgtca gcggttgtca cggatttaac ttatgttcgg gtcgggacaa attgggctta
      301 tatttgtttg atgattgatt tattcaatcg tgaaattatt ggccattctt gcggaaataa
      361 aaaagatgcg cagcttgtaa aaagggccat tcaaagcatt ccttactcac ttcaagaaat
      421 cgaactcttc catacggatc gtggaaaaga atttgataac caaaccattc agaatctcat
      481 gaatggtttg gttatcaaat tcctttcc
//
LOCUS       DADXTG010000331          506 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.343, whole genome shotgun sequence.
ACCESSION   DADXTG010000331 DADXTG010000000
VERSION     DADXTG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.343"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(426..499)
                     /locus_tag="IWI57_002880"
     tRNA            complement(426..499)
                     /locus_tag="IWI57_002880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Asn,seq:gtt)
BASE COUNT          163 a          100 c          104 g          139 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa
      241 atcaggtgca tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc
      301 agtgggagca tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta
      361 cactcttagt aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac
      421 ttttactccg gcagtaggac tcgaacctac gacatcatga ttaacagtca tgcgctacta
      481 ccaactgagc tatgccggaa taacat
//
LOCUS       DADXTG010000332          485 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.344, whole genome shotgun sequence.
ACCESSION   DADXTG010000332 DADXTG010000000
VERSION     DADXTG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.344"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            54..467
                     /locus_tag="IWI57_002881"
     CDS             54..467
                     /locus_tag="IWI57_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBC2450949.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          185 a           81 c           85 g          134 t
ORIGIN      
        1 ttattacgta aatagggata aataaaaaat acttattgaa aggatgatca aaaatggcaa
       61 atgtacctag cattcgcgac atgttccctg atttcaatga cgtgtttagt ccagcattca
      121 atgatttttt aggtgtttca agttatccaa aagtagattt agtcgaaaat gaaaaagaat
      181 acaaactaac tgctgatatg cctggctgtg acaaagaaga cacgacagtt gaatattcag
      241 ataatacttt gacgatctcc gccaatcatg agtcccatac agaagataaa gaagatggca
      301 actatgtacg gaaagaaaga cattcggtct cttacaaacg ttcattctat cttcctaatg
      361 ttgatgaaga aaaaatcact ggaaccttta aaaacggtgt gctgaaattg gtattaccta
      421 aaacagctta tcagccaaaa gaaacaaaaa aaattgagtt aaattagaac ttatcgcctc
      481 tcgat
//
LOCUS       DADXTG010000333          483 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.345, whole genome shotgun sequence.
ACCESSION   DADXTG010000333 DADXTG010000000
VERSION     DADXTG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.345"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..419
                     /locus_tag="IWI57_002882"
     CDS             <1..419
                     /locus_tag="IWI57_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBC2450950.1"
                     /translation="EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          164 a           97 c           82 g          140 t
ORIGIN      
        1 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
       61 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
      121 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
      181 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
      241 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
      301 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
      361 tttacaattt caaactacga attatgattc gtcatggcaa aacattttta accaaataaa
      421 cagaacagaa agaggttttc ttctttctgt tctgtccaaa ttattctaag atttctcttt
      481 acc
//
LOCUS       DADXTG010000334          480 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.346, whole genome shotgun sequence.
ACCESSION   DADXTG010000334 DADXTG010000000
VERSION     DADXTG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.346"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IWI57_002883"
     tRNA            complement(20..91)
                     /locus_tag="IWI57_002883"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IWI57_002884"
     tRNA            complement(113..200)
                     /locus_tag="IWI57_002884"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IWI57_002885"
     tRNA            complement(225..298)
                     /locus_tag="IWI57_002885"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IWI57_002886"
     tRNA            complement(312..382)
                     /locus_tag="IWI57_002886"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IWI57_002887"
     tRNA            complement(384..459)
                     /locus_tag="IWI57_002887"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          135 a          128 c          112 g          105 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
//
LOCUS       DADXTG010000335          479 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.347, whole genome shotgun sequence.
ACCESSION   DADXTG010000335 DADXTG010000000
VERSION     DADXTG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.347"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..479)
                     /locus_tag="IWI57_002888"
                     /pseudo
     CDS             complement(1..479)
                     /locus_tag="IWI57_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          167 a           78 c           73 g          161 t
ORIGIN      
        1 acttcttttt taaaaaaagt attcatcata aaagccacta cttgcggatc ttcatcataa
       61 aattctgtag cagtacttga aaagatgttc caataaatat ctaatgctgc atattgaatt
      121 tgtacctctt ttccagaaaa agactttctg ccattattag aaaaggagat agacaaaccg
      181 tactatgcta aaaatgcatt aatctttttc attttcgtat acaaataaga ggcactatag
      241 tttgtttgct gactgaaact gataatcgaa tttgtctgac agctgaacaa agaagtcaag
      301 atctgataac tgactgattc gttgcagtat cttccaaata gacgataata gcagtaagcc
      361 ggatcaattc cttcaaaatt caactttaaa aaaggttttt gcttatcaat atgcagttct
      421 gttagattga gacgctcaaa gtcttgttct aattctttga tgtatagtaa aaaatttga
//
LOCUS       DADXTG010000336          479 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.348, whole genome shotgun sequence.
ACCESSION   DADXTG010000336 DADXTG010000000
VERSION     DADXTG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.348"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..>479)
                     /locus_tag="IWI57_002889"
     CDS             complement(38..>479)
                     /locus_tag="IWI57_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450951.1"
                     /translation="EYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKII
                     IDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWL
                     DPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          144 a           87 c           78 g          170 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactct
//
LOCUS       DADXTG010000337          449 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.349, whole genome shotgun sequence.
ACCESSION   DADXTG010000337 DADXTG010000000
VERSION     DADXTG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.349"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          158 a           74 c           71 g          146 t
ORIGIN      
        1 atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat
       61 taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca atttttgaat
      121 aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt agcgacaaat
      181 acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc agcaaataat
      241 gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg tgcttaccca
      301 aagcaattca aaaatttcat aactactatt ttaggagatg attttataga atccagagtc
      361 tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac tctagataag
      421 tggattcgta tatataattt cccagtaat
//
LOCUS       DADXTG010000338          449 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.350, whole genome shotgun sequence.
ACCESSION   DADXTG010000338 DADXTG010000000
VERSION     DADXTG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.350"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>449
                     /locus_tag="IWI57_002890"
     rRNA            <1..>449
                     /locus_tag="IWI57_002890"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          136 a           99 c          132 g           82 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggca
//
LOCUS       DADXTG010000339          446 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.351, whole genome shotgun sequence.
ACCESSION   DADXTG010000339 DADXTG010000000
VERSION     DADXTG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.351"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            333..>446
                     /locus_tag="IWI57_002891"
     CDS             333..>446
                     /locus_tag="IWI57_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBC2450952.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          152 a           62 c           81 g          151 t
ORIGIN      
        1 agtattaaat atactgcaag gcaagacatt tggtaattga agaataacaa gcaaaaaaat
       61 agcagtcagg tctcttgaca aagtatagtt gctattcttt ttttaggccc tactttaatg
      121 aaatttttgt ttggagggaa cgacttcatt cctttgatta tttcagttga atttaataga
      181 gaagcctggc gtctattaag aaatactttt cttgatgagc gtcaattttt ttaacggaaa
      241 tctatcctat actaaattcg gttctgttgc aaagttttcc aaaaaatcta ttttagtgta
      301 aaattgagaa aaaagacaga gaggacagag taatgaatca ttttaaaggc aaacaattca
      361 aaaaagacgt cattattgtc gctgttggtt actacctgcg ttacaatcta agctatcgtg
      421 aagttcagga attgttatat gatcgt
//
LOCUS       DADXTG010000340          440 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.352, whole genome shotgun sequence.
ACCESSION   DADXTG010000340 DADXTG010000000
VERSION     DADXTG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.352"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          170 a           68 c           65 g          137 t
ORIGIN      
        1 tcctaaaaaa tctaaactct ttagatatga gtaatgtcat tttaaactat atcgatgctc
       61 aaaaacgtaa aatttgggat caagatctgt ctaggaaaga atttctatag tttaactgaa
      121 taacacaaaa aggcatatca tgcgacaaga aaaacaatga aaagagaaat ttgccatcac
      181 tggaacatcc atacagaaat tagcatagat tctgaaaaat ctaccgctag tttttattta
      241 gttgttctaa cgacaccaat acttgtaatg atcgaaaatg tgcaattggg tcagatagtg
      301 aaccaaagta gaatctcaaa gcgacaatta tgaatgtaaa agtacctttt atgattagtt
      361 tattctctaa ttgattacat cccgtataat taataaaatt actttaccga ttacacgatc
      421 ttatgtcagt agagctttta
//
LOCUS       DADXTG010000341          439 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.353, whole genome shotgun sequence.
ACCESSION   DADXTG010000341 DADXTG010000000
VERSION     DADXTG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.353"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          142 a           79 c           55 g          163 t
ORIGIN      
        1 tcgtattttt gttacaaaat tatcgaaaaa aataaacatg tgccatctat ctgttggcac
       61 atgttttagg atttatggta ctttttattc ggcaatccat tttatttttc tacttcttct
      121 taatacctct aacacttttg atttataaac taatccaatg catctacttg cgcttattaa
      181 gctatttcag ctagatagta ccaccccatg cgtcaatgct tgattttcct tttgtataac
      241 attgtataag caataaagga caaataagtg gtgattacac caaaccattg aataagaaaa
      301 attctgatac gtaaataaac atgaacttga aaaaattaat gaatcggtta gctactacgt
      361 agttgtcttt tttctcatac agttctgtta acttacactt ctcttcgcta gataataaaa
      421 aatcctccaa ctgtattgt
//
LOCUS       DADXTG010000342          413 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.355, whole genome shotgun sequence.
ACCESSION   DADXTG010000342 DADXTG010000000
VERSION     DADXTG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.355"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>413
                     /locus_tag="IWI57_002892"
     CDS             <1..>413
                     /locus_tag="IWI57_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBC2450953.1"
                     /translation="IAYVEQKADIDFTFPIKVKECVSMGTYAGMKVFQRIKNAEWQRV
                     SKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEPFVGIDLVSERII
                     MDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLN"
BASE COUNT          158 a           43 c           83 g          129 t
ORIGIN      
        1 agattgcata tgtagagcaa aaagctgata ttgattttac ttttccaata aaagtaaaag
       61 aatgtgtatc tatgggaaca tatgccggaa tgaaagtttt tcaaagaatt aaaaatgcag
      121 aatggcaaag agtttcaaag gctttggaaa aggtcgatat gggaaaatat tctaatcatc
      181 aaataggaga attatctgga ggacaatttc aacgtgtact tttagcaaga tgtcttgctc
      241 aaaatgcgga tttcatattt ttagatgaac catttgtagg gatagactta gttagtgaac
      301 gaataattat ggatacgtta aaaaaattga aacatcaggg aaaaacagtg ctaattgtgc
      361 atcatgattt aagcaaggta aagaagtatt ttgataatat tattcttttg aat
//
LOCUS       DADXTG010000343          398 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.358, whole genome shotgun sequence.
ACCESSION   DADXTG010000343 DADXTG010000000
VERSION     DADXTG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.358"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..305
                     /locus_tag="IWI57_002893"
     CDS             <1..305
                     /locus_tag="IWI57_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBC2450954.1"
                     /translation="TIKVSFQRWDYKGFVTFRRGGNCKGLDVLALDEDDLYDQTLTDN
                     PIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSFEIIEFVADKEE"
BASE COUNT          142 a           39 c           93 g          124 t
ORIGIN      
        1 atactattaa ggtttctttc caacgttggg attacaaagg atttgtgacg tttagaagag
       61 gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta tacgatcaaa
      121 cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa ggtaatgaat
      181 ggtttaaaat gacgcttatg aatgataatg gagacgaatt atctgttgaa gatacttggt
      241 catatttgag tgattatatt gtcagctttg aaatcattga atttgtagcg gataaagaag
      301 aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca cgatgattaa
      361 taaaaaggta gtattacctt aggcattgag taaggaat
//
LOCUS       DADXTG010000344          387 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.359, whole genome shotgun sequence.
ACCESSION   DADXTG010000344 DADXTG010000000
VERSION     DADXTG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.359"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            260..>387
                     /locus_tag="IWI57_002894"
     CDS             260..>387
                     /locus_tag="IWI57_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2450955.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQK"
BASE COUNT          139 a           60 c           59 g          129 t
ORIGIN      
        1 gttttagaaa agatttatta tacgtaaaaa gctcaagaca ttttcttagc cataaggaca
       61 tgtttgatat ttaagaagat atatgaattt tcttttctaa gtataaagat aactatccat
      121 tatcaactaa aaaaacattt ttgaaaataa acatcagcag tcctatcaat gcatgtgagc
      181 ttatagaagt tattcgcagc gcagcataat tgcattagct tcttttcgtg ttttttgcct
      241 cttttccctt taaatcttaa tggaggaggt gtgcaatatg tcagataacg acgaacgcgt
      301 aaatgcccag ttgattcgat tctttgaaaa ggtcattttg aacaccgcta atacttatta
      361 taaaaaacaa gaagaaattc aaaaaca
//
LOCUS       DADXTG010000345          386 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.360, whole genome shotgun sequence.
ACCESSION   DADXTG010000345 DADXTG010000000
VERSION     DADXTG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.360"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>386)
                     /locus_tag="IWI57_002895"
     CDS             complement(<1..>386)
                     /locus_tag="IWI57_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBC2450956.1"
                     /translation="ARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNLETFQHSAII
                     ISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPRLVIL
                     LSFVLGTLFLSIYRILVYKLYLRVSR"
BASE COUNT          152 a           75 c           64 g           95 t
ORIGIN      
        1 tcgacttacg cgtaaataaa gtttatatac gagaatacga tagatactta agaaaagcgt
       61 accgagaaca aatgaaagta agataacaag acgtggaaaa gcaaaccagc ggcccgcaaa
      121 agtcactacc atgatcccta gtgtcatcaa tacctgaccg atgactgtga caaacacgat
      181 gtcactgatc attcgattat aaaaaacata tgcacccaac aaaatgttca acgcaataaa
      241 aatcactgaa ataataattg ctgagtgttg gaaagtttct aaatttcgta atgggatctc
      301 tccacgaaac ttcagtaaga aactactata tattgctgca ttgtatacaa taacatccat
      361 taatataatg aatatccgtc gtgcag
//
LOCUS       DADXTG010000346          368 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.362, whole genome shotgun sequence.
ACCESSION   DADXTG010000346 DADXTG010000000
VERSION     DADXTG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.362"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..231
                     /locus_tag="IWI57_002896"
     CDS             <1..231
                     /locus_tag="IWI57_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBC2450957.1"
                     /translation="EQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFND
                     AEIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            225..>368
                     /locus_tag="IWI57_002897"
     CDS             225..>368
                     /locus_tag="IWI57_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450958.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNG"
BASE COUNT          130 a           52 c           69 g          117 t
ORIGIN      
        1 gaacaagatg tctatttctt atttggaatg gaaatattaa tttcagatca aaagttatca
       61 gcagcaattg atcagttatc ggagataaga agaaagatta tcctacttta ttattttgca
      121 ggttttaatg atgctgaaat cggtaaaatt ctcaatatga gcacaagtgg tatctggtat
      181 caacgaaaga aggcagttga acaattgaaa atggggtatg acctatggta gataaatcta
      241 aaaagcggca gtacctatta gttccactat ccattatctt atctgctcta gaaggagatc
      301 caatttcgat caagatgata ctcaaacatt atcattctta tatggctaag ttatgtttga
      361 caaatgga
//
LOCUS       DADXTG010000347          367 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.363, whole genome shotgun sequence.
ACCESSION   DADXTG010000347 DADXTG010000000
VERSION     DADXTG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.363"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>367)
                     /locus_tag="IWI57_002898"
     CDS             complement(<1..>367)
                     /locus_tag="IWI57_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450959.1"
                     /translation="LKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFF
                     SDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFE
                     NQFNLLREETSQLIRRELTK"
BASE COUNT          117 a           64 c           52 g          134 t
ORIGIN      
        1 tttggttaac tctcttcgga tcaattggct cgtctcttct cgcaacagat taaactgatt
       61 ttcaaaactt tcaatgaatc gttcattcaa ccattcgatt gtttcatctt cattttttct
      121 caagtatttt tgatccaatt tatcatgaag ataaagccaa gagagtgtct tattgataaa
      181 aataacggat ttttctccta atactccttt taagtggtaa aatcctatat ccgaaaaaaa
      241 tagcgactga ttgctagcaa tatctaaaga atcagcttgt tcagccaaca ggttgattgc
      301 taaaaagtgg aataatttct ttttttcata agcagacaga ggataatact gttttaaatc
      361 ttttaac
//
LOCUS       DADXTG010000348          364 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.364, whole genome shotgun sequence.
ACCESSION   DADXTG010000348 DADXTG010000000
VERSION     DADXTG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.364"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>364
                     /locus_tag="IWI57_002899"
     CDS             <1..>364
                     /locus_tag="IWI57_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBC2450960.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILST"
BASE COUNT          137 a           62 c           76 g           89 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 caca
//
LOCUS       DADXTG010000349          359 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.365, whole genome shotgun sequence.
ACCESSION   DADXTG010000349 DADXTG010000000
VERSION     DADXTG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.365"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>359
                     /locus_tag="IWI57_002900"
     CDS             <1..>359
                     /locus_tag="IWI57_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBC2450961.1"
                     /translation="RDVERDLAINALILGSGLRLSEVVNINLEDLSLEKNNVVVTRKG
                     NKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIYQGEAKRISGIAIERMVA
                     KYSKGFRVQVSPHKLRH"
BASE COUNT          141 a           51 c           75 g           92 t
ORIGIN      
        1 aacgagatgt agaaagagac ctggcgatca atgcattgat tttaggatct ggattaagac
       61 tctccgaggt tgtaaatatt aatttagagg acctatcttt agaaaagaat aatgtggtag
      121 taactagaaa aggaaataag agagacgcag taaatatagc agcatttgca atggaatatt
      181 tagctaatta tctagctatt cggaaagaac gatacaaagt aacagaaaac gaaaaagctt
      241 tatttctagc aatttatcaa ggagaagcca agcgtatatc cggtattgca atcgaacgga
      301 tggtagcaaa atattcaaaa ggattcagag tccaagtcag tccacataaa ttacgtcat
//
LOCUS       DADXTG010000350          354 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.367, whole genome shotgun sequence.
ACCESSION   DADXTG010000350 DADXTG010000000
VERSION     DADXTG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.367"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>354
                     /locus_tag="IWI57_002901"
     CDS             <1..>354
                     /locus_tag="IWI57_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HBC2450962.1"
                     /translation="QEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDA
                     DGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQ"
BASE COUNT          122 a           66 c           73 g           93 t
ORIGIN      
        1 gcaagagtac agcaaagtcc tctattatct ttggaagaag aaaaatagac aatccttcta
       61 ttcatggaaa atggacgaaa cctatatcaa aattaaggga cgttggcatt atctttatcg
      121 tgcaattgat gcggacggct taaccttaga tatctggtta cgaaagaaac gggatacgca
      181 agcagcctat gctttcttaa aacgactcca taaacagttt ggtgagccga aagcaattgt
      241 gaccgataaa gcaccttctc ttggctccgc ctttagaaag ttacagagtg tgggtttata
      301 tactaagaca gagcaccgaa ctgtgaagta tcttaacaat ttaatagaac aaga
//
LOCUS       DADXTG010000351          353 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.denovo.368, whole genome shotgun sequence.
ACCESSION   DADXTG010000351 DADXTG010000000
VERSION     DADXTG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.denovo.368"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(120..281)
                     /locus_tag="IWI57_002902"
     CDS             complement(120..281)
                     /locus_tag="IWI57_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBC2450963.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          123 a           68 c           38 g          124 t
ORIGIN      
        1 aaacaagaat acaagcaaga aagaactata gttaatatga taaaaattat taaagttcta
       61 tttttctttc ttgtaatata tgctatagca ttttttatca ctttcctaac tactcctctc
      121 tattttctta ctccataact aattgcatcc actggacaag catttttaca tcgtccacat
      181 ctgatacact ccagatgatt gcaattctca actgaatctt tattcatttg gcaaactttt
      241 ttacattttc cacaattaat acacttatcc ttatcctgaa tcggctacac tagactagac
      301 agaaaaaata aggtgtgtag aataataaaa aacacactgg aggatttttc atg
//
LOCUS       DADXTG010000352        20728 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.040, whole genome shotgun sequence.
ACCESSION   DADXTG010000352 DADXTG010000000
VERSION     DADXTG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.040"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..280
                     /locus_tag="IWI57_002903"
     CDS             <1..280
                     /locus_tag="IWI57_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBC2450964.1"
                     /translation="SGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFK
                     GTYDTPGVSHVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            303..554
                     /locus_tag="IWI57_002904"
     CDS             303..554
                     /locus_tag="IWI57_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBC2450965.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            544..1773
                     /locus_tag="IWI57_002905"
     CDS             544..1773
                     /locus_tag="IWI57_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBC2450966.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            1770..3851
                     /locus_tag="IWI57_002906"
     CDS             1770..3851
                     /locus_tag="IWI57_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBC2450967.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            4000..7920
                     /locus_tag="IWI57_002907"
     CDS             4000..7920
                     /locus_tag="IWI57_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBC2450968.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            7921..8865
                     /locus_tag="IWI57_002908"
     CDS             7921..8865
                     /locus_tag="IWI57_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450969.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            8869..9072
                     /locus_tag="IWI57_002909"
     CDS             8869..9072
                     /locus_tag="IWI57_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBC2450970.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(9372..9608)
                     /locus_tag="IWI57_002910"
     CDS             complement(9372..9608)
                     /locus_tag="IWI57_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBC2450971.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(9791..10177)
                     /locus_tag="IWI57_002911"
     CDS             complement(9791..10177)
                     /locus_tag="IWI57_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBC2450972.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(10159..11487)
                     /locus_tag="IWI57_002912"
     CDS             complement(10159..11487)
                     /locus_tag="IWI57_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBC2450973.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(11448..11777)
                     /gene="mobC"
                     /locus_tag="IWI57_002913"
     CDS             complement(11448..11777)
                     /gene="mobC"
                     /locus_tag="IWI57_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBC2450974.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(12035..12406)
                     /locus_tag="IWI57_002914"
     CDS             complement(12035..12406)
                     /locus_tag="IWI57_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450975.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            12551..12736
                     /locus_tag="IWI57_002915"
     CDS             12551..12736
                     /locus_tag="IWI57_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBC2450976.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            12894..13061
                     /locus_tag="IWI57_002916"
     CDS             12894..13061
                     /locus_tag="IWI57_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450977.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            13248..13466
                     /locus_tag="IWI57_002917"
     CDS             13248..13466
                     /locus_tag="IWI57_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450978.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            13679..14338
                     /gene="vanR"
                     /locus_tag="IWI57_002918"
     CDS             13679..14338
                     /gene="vanR"
                     /locus_tag="IWI57_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBC2450979.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            14341..15684
                     /gene="vanS"
                     /locus_tag="IWI57_002919"
     CDS             14341..15684
                     /gene="vanS"
                     /locus_tag="IWI57_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBC2450980.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            15855..16661
                     /gene="vanY"
                     /locus_tag="IWI57_002920"
     CDS             15855..16661
                     /gene="vanY"
                     /locus_tag="IWI57_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBC2450981.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            16679..17506
                     /gene="vanW"
                     /locus_tag="IWI57_002921"
     CDS             16679..17506
                     /gene="vanW"
                     /locus_tag="IWI57_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HBC2450982.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            17503..18474
                     /locus_tag="IWI57_002922"
     CDS             17503..18474
                     /locus_tag="IWI57_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBC2450983.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            18467..19495
                     /gene="vanB"
                     /locus_tag="IWI57_002923"
     CDS             18467..19495
                     /gene="vanB"
                     /locus_tag="IWI57_002923"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBC2450984.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            19501..20109
                     /locus_tag="IWI57_002924"
     CDS             19501..20109
                     /locus_tag="IWI57_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBC2450985.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(20175..20348)
                     /locus_tag="IWI57_002925"
     CDS             complement(20175..20348)
                     /locus_tag="IWI57_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBC2450986.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         5225 a         5213 c         5655 g         4635 t
ORIGIN      
        1 cagcggcttc atttcgtggg taatcaatca tagtggatgg aacgtgggac ggctgggggc
       61 gcagggccta tataacatct gcacccctgt ttccagttcg caggccaagc ccggcgacct
      121 tgtatttttc aagggaacct acgacacccc cggcgtgtcc cacgtcggcc tgtatgtggg
      181 caacaatgtg atgctgcatt gcgggaaccc gatttcctac acgaacctta cttcaaacta
      241 ctggcaatcc catttttaca gttacgggcg tttaccttaa cgccagaaag gagctttcaa
      301 ttatggcaac caaacttgac cgcatcgaaa aggacatcca gaaaaccaag tctaagatcg
      361 cggagtttca gaaacagctt cgggagctgg aaacgcagaa aaccgaacag gagaatttgc
      421 agatcatcca gcttgtgcgc ggcatgaaca tgaagccgga ggaatttgcc gcgttcttac
      481 gcagcggcgc aatgcaggcc gcccctgccg caacccctta ccatgaacag gaggacaatg
      541 cccatgaaga ataagaccag acggatttcc cgtattctcc ccgcacttct ggccgtgatg
      601 ctctgcgtca cggcctttcc cgttaccgcc cttgcaggcg gcaatgaccc ggcaccggcc
      661 ccgctgccgg aggctacaac cgaacccgct acgggcggtt tggagcctga aacagacgaa
      721 accggcaccg ccacggacgg gacagaaacc gaggccgaaa aagataccgg ccctctgacc
      781 ggcaaggaca tcaccgacct gttttccgcg ctgttcggtt ccaaggtgag cattgccgcc
      841 acggacgacg gcatccagat caccaccggc accgacaaaa aagaacctga acagaccggc
      901 accgtcacca ccaacggcgg caggctgaat gtccgcaccg gcgcggggct tgacaagacc
      961 gcgttcaccc agcttcccaa tgggacgacg gttgaggtag tcggcacaga cggcgactgg
     1021 attaaaattc tcttgccgga gcgcatcggc tatgtccatt ccgactatat gaccgtcagc
     1081 gaaaaggaag tagcggctac cggcgagggc ggtttttccc tctccattga cccggaggaa
     1141 attgcttctc tgctggaact gttcaatggc ggcggggcca gcgccgccct gtccccggac
     1201 gggaacctgt cactgattga cgacatcggc agcgccgcga aaaatggcaa gcagtttatc
     1261 accgtggaaa ccaaaaacgg caatgtgttt tatctgatta ttgaccggga cgacgaggga
     1321 aaagaaactg tccatttctt aaatcaggtg gatgaagccg atctgctgac gctgatggag
     1381 gacggggaaa ccgtcacccc cgctgctgtc tgctcctgta ccacgaaatg caaggccggg
     1441 gctgtgaaca cgaattgccc ggtatgtaaa accaacctga ccgagtgcag cggcccggag
     1501 ccgcaggaac cccagccgga ggaacccgaa gccccggagg aagaacccaa gggcggcgcg
     1561 ggcggcctga tcgtgttcct gctggtggcg ctggcgggcg gcggtgcggc cctttactat
     1621 ttcaagttca aaaagcccaa ggctgaaacc accggcagcg acgatttggg cgagtatgat
     1681 tttggggagg acgaggattt ggaggacgag gaacccgaaa ccgagtttga cccggaagct
     1741 gattttgtga gtgaacagga ggacgaggaa tgacatataa acttgtaatc gcggaaaaac
     1801 ccagcgtggc ccgcagcatt gcgggcgtga tcggggccac ggagaaacac gacggctatt
     1861 tggaggggaa cggctatctg gtgagctggt gcatcggaca tctggtgtcc tttgccgacg
     1921 ccggacgcta tgacgagcgt ttcaaaaaat ggcgctatga ggatttgccc attctcccgg
     1981 aaacatggca gtacatcgtc cccgacgaca agaaacagca gtttgacgtt ctctgttcgc
     2041 tgatgcagcg ccccgacgtg accggccttg tgtgcgccac cgacgccggg cgcgaggggg
     2101 aattgatttt ccgtttcgtc taccagatgg cgggatgcca aaagcccttt gagcgccttt
     2161 ggatttcctc tatggaggac agtgctatta aggacggctt tgcccacctg aaaccgggcg
     2221 gcgactatga tccgctgtat caatcggccc tgtgccgggc caaggccgac tggctgatcg
     2281 gcattaacgc cacccggctt ttttccgtcc tgtaccacaa gaacctgact gtgggccgcg
     2341 tccagacgcc gaccttaaaa atgctggtgg acagggacgc caagattacc gatttcaaga
     2401 aagagaaata tcacatcgtc catatcacgg cgggcggcgc ggacgcggta agcagccgct
     2461 tttccgacgc tgccgaggca aacaccgtca aggcggcttg tgcaaaagcg caggccgttt
     2521 gcgtttccgt gacgcgggag aaaaagacgg aacagccgcc caagctctat gaccttacca
     2581 ccttgcagcg ggaggctaac cggcttttcg gctataccgc caagcagacc cttgactatg
     2641 cccaggcact gtatctaaag aagctgctga cctatccgag aacggatagt caatatctta
     2701 ccgacgatat gctgcccgcc gtggaaagcc ttgtgtccgg cctgtgggag ctggtgccgt
     2761 ttgccaaggg cctgaacctt tccccgcagt ttgaccgcat cctgaacagc aaaaaggtgt
     2821 ccgaccacca cgccattatc cccaccgctg aatttgtgaa gcagggcttt ggcactctgg
     2881 ccgagagtga aaaaaagctg ctgtccctga tctgctgcaa gctcttatgt gccgtggccg
     2941 ggccgcatat ctatgaggcc gtcaccgcca catttacctg tgccgggcag gagtttaccg
     3001 ccaagggcaa gaccgtcctt tcccccggct ggaaagagat tgaacgccgg ttccgttctt
     3061 ccttaaagac cgacgccgac gaggacgccg aggcggtgcg ggagctgccg gagctttgcg
     3121 agggccagac ctttgcagac gtggccgcct ctgtcaccga gcatttcacc acgccgccca
     3181 agccctacac cgaagatacg cttttgtccg ctatggaacg ggccggggcc gaggatatgc
     3241 cggaggatgc cgagcgcaag ggattaggca caccggccac ccgcgccgcc attctggaaa
     3301 agctggtgca gatgggcttt gtccagagaa aaggcaagca gctcattccc accaaggacg
     3361 gcatcaatct ggctgtggtg ctgccggagg cgcttacttc tccccagctt actgccgaat
     3421 gggaaaaccg gctgaccgag atcgccaagg ggcaggccga tcctgacgaa tttatggacg
     3481 gcatcgagac gcaggcgcgg gagctggtgc agacctattc ctgtatcagt gaggaaaagc
     3541 agaaattgtt tcaggccgag cgtgttccta tcgggacgtg tccccgctgt ggtgaggctg
     3601 tctatgaggg caagaaaaac tactactgcg gaaaccgggc ctgccagttc gtcatgtgga
     3661 agaatgaccg attttttgag gaacgcaaga aagcgttcac cccgaagatc gccgctgcgc
     3721 tgctcaaaac cggcaaagtc aaggtaaagg gcctctattc cgtaaagacc ggcaaaacct
     3781 acgacgggac ggtgcttttg gctgataccg ggggcaagta cgtcaactac cgcattgaac
     3841 acaggagcta aaggacagta acacagcaag catagggagt gtggctacac tcccggtaaa
     3901 atcccccggc cgcgtccctg ccgggccggg gatttttgct tgttggggaa gtccccaacc
     3961 ccctattttt tgaaacagaa cagagaaagg agtgaccgcg tgaacgaagt attttctatc
     4021 caagtccata accggcagtt tttggaacag ggcaaacaag gcgtctggct ggatttgccc
     4081 accacaaccg aaacgctgca agccgccctg cgggatgttg gcattactgc cgacaatccg
     4141 caggattttt ttatcaacgg gtattcctgc ccggaggaca gacaccttgc cattccctat
     4201 gacatggtgc tggccgccga cgtggacgag ctgaatttcc ttgccgcccg gctgggccag
     4261 cttgacgccg ccgagatcgc agagctgaac gccgccctgc aaaatccccg tggcggcttt
     4321 gagaatatcg gccagatcat cgactacacc gaaaatgtgg attactatgt gcatcttccc
     4381 gacgttaggg gggatggaca gttgggcgac tactacctga accgttccgg catggtggat
     4441 atgccggagg aatggaaagc cgggattgat acggcgcagt ttggacgcca cattgcccag
     4501 caggaacaag gcgtttttac ccagtacggc tatctggtga gaagcggcga cgaatggcag
     4561 cgcgtccatg agggccagcc ggtgccggag gaataccggg tgatgcacta cccccagccg
     4621 gaagctgacc gggaggaacc gcaggcccag cagccggagg catccgaaag ccccgacaaa
     4681 gcgccgcccc cggttatccc gatcatgctg gacggcatga acagcaaaga acgcatgaaa
     4741 gtgattactg accgtctgga aaccggcgta caggagcttt ttgaaagtag ccgctatatg
     4801 gcttacttat ccgctatgtc caagatgcac gattacagtc tgaacaatac gctgctgatc
     4861 gccatgcagg gcggttcgat tgtggcgggc tataacaggt ggcaggatga atttgaccgc
     4921 catgtgaagc ggggcgagaa aggcattaag atactggccc cggccccgtt caaggttaag
     4981 cagcagatgg agaaaatcga cccggccacc cagcagcccg tgatcggcgc ggacggcaag
     5041 cccgtgaccg aagaacagga agtgacggtt ccggccttta aggtggtgac agtctttgac
     5101 atcagccaga ccgagggcaa ggattttccc atgccctata tcagtgaact taccggcgac
     5161 gtgcagcagt atcaggattt ctttgccgcg ctggaacgga catcacccta tcccataagc
     5221 attgagccga tttcagacgg ggccaagggc cgctgcttct acgcaaaaca gcgcattgcc
     5281 atcaacgagg gcatgagcga attgcagacc ctaaagaccg ccatccatga gattgcccat
     5341 gccaagctcc acgccattga ccgggaggcc cccgccgagg aacaggcggc ccggccagac
     5401 cgcagcaccc gcgaggtaca ggccgaaagc gtggcctata ccgtctgcca gtattacggg
     5461 ctggacacgt cggactattc ttttggctat gtggccgatt ggagcagcgg cagggagtta
     5521 tccgagctga aagcctccct tgaaaccatc cgggccacgg ccagcgaatt gataaccgag
     5581 atcgacggcc attttgccca gcttcagcag gagcgcgagg cccggcagga acaggaggcc
     5641 gtcacggttg acgttacccc ggaggcagcg gccccggcgc aggccgagga agaacaggcc
     5701 ccggcccttg atccggcagc cgagccgctt gtgactgtgc tatggagcga aagcccccag
     5761 ctaaaggacg gccagcaaat gccgttgcat gaggccgacg cgctgtttgc ccgtctggat
     5821 gcagcatatc cggcagaggc aggctatgac aaaaccgcgt tccgcattga ctgtatcttt
     5881 cagggcgagc cggacagcta cgaggggcgg caggattttg gagataggga cggttccctg
     5941 atcgaacata tccagagcta ccaccaattt tacgcgcagg acgaaaactg gaaaaagcat
     6001 gtaatcgcta caaccggcat agagggctgg gagcaggaca aggcccatcg ggatatgctg
     6061 ttaggcgagt ttgtccccta tatgaagctg cattgcagcc ttgcccaaat ggaacagacg
     6121 gcgcagggcc gcttacagtc cggggaaacc ctgacgccgg aggaaacggc ctatttgggg
     6181 gcgcttctct cttatgtgca ggagtgccgc cccatgctca atcagggcca gtatcagttg
     6241 ccggagccgc cgaaactggc tgacttcgac cagagcttgc aggactacaa agcacaggta
     6301 caggccgaga tcgcgcagga ggccgccgca gccggtatga ccgtggagga atacgcggcg
     6361 gctggctatg aagcacccca acaggacacc ttttccatct accagcttcg aggcggggag
     6421 gaaacccgcg actatcggtt tgagccatac gagcgtttac aggccgcagg gctggccgtg
     6481 gacagtgcca actatgacct tgtttatacg gcaccgctgg ataaaagcac gtctttggag
     6541 gatatttacc gcacattcaa catcgaccac ccggcagatt ttaggggaca cagcctttcc
     6601 gtttccgacg tggtggtgct gcaccagaat ggacaggaca ccgcccacta tgtagacagc
     6661 ttcggctata tggaagtgcc ggagtttttg cgggaaaatc ccctgcgtac cgccgaactg
     6721 tctacggagc agaacgaaaa catgattgac ggcattatca acaatacgcc gcccgaaccg
     6781 cagccggagg aacccgaaca gctatctttt gaggatgtgg cagttgcttc cggccagaca
     6841 caggaggaac ccccggaggc tgcgcccccg caaacggctg tccgctatta tcccatcaat
     6901 gagggagccg cccgccgcgc caaggaagcc atgagctttt ctgactaccg gcccggcagc
     6961 gccacggcgg aataccggca ctatgtcgat caggccgtgg aaattgcgga gcgccagaaa
     7021 aagcgtgtgg agccagagta tcacgaaaag atagacagcc tccttgacac ctacgcccgc
     7081 aagctggccg agaacatgaa taagggctat gagattaccg cccgcgtccc ctctgtcctg
     7141 attgccgggc cgtccaattt cccggtacgg gcgaaagaaa agcagaacgc cgccagtgac
     7201 cgcaacatgg aggaatttca gtacattcag ggcttgcttg acaaaatccg cagcaccggc
     7261 atgggtggta tcagcgccga cgatccgcag gccgtttcca agctggaaaa gaaactggaa
     7321 aagctggaag cgtcgcagga gttgatgaag gccgtcaatg cctactaccg caagcatggg
     7381 acgctggacg gatgccccca tctgaccgaa agaggcattg aaaacctgaa agcggatatg
     7441 gcgtcgggct ggcactatga gaaaaaaccg ttccagtcct ggcagctctc caacaataac
     7501 gccgagatac gccggttaaa gggccgcatt gaggaattga cccgccaaaa ggaggcggcc
     7561 tatgtgggat gggagtttga cggcggcact gtggagatca accgggtggc caaccgcctg
     7621 caaatcttct ttgaggggaa gccggatgct gctgtacgcg acgagctgaa aagcaacggt
     7681 ttccgctggt cgcccaaggc cggggcatgg cagcggcagt taaacgacac cacaatccgc
     7741 gtcgctgacc gtatcaagtg catccagccc ctttccggcg aaaagcccag cgccttacag
     7801 cttgcggccc gcagggagaa agaaaagccc tctatccggg cgcagctcaa cgcggccaag
     7861 accgaacagg ccaagcagcc accggcgcgg gaaaaatcaa agggattgga ggtagagtaa
     7921 atgcagaagt ttgaaaatgt ggacgttctg gccgcgctgg aacagatcat gcggcagaac
     7981 accgcgtttt atcaaagtga ctttggcatt gacaaagaca ttatcaggcg ggatgccgcc
     8041 agcgatcagg ccgcagacaa gaccctgctc tggatgtccc ggccctccgg gacgtactgt
     8101 ttccgggaac gtgatgtttt cctgaaagac acccgacagt acaacacatg gaaattttac
     8161 ggggagcaga cccgtgacaa gatactggcc tacgcggtgg agctgaccgg cacacagggc
     8221 ggcactatcc ggggcaatct ctatgagctg gactatccgc agcatttccg gcacgtcatt
     8281 gaaaccgccc agccggtgag tgtgaaccgg ctcttttatg agcatggcac ccgtgacatc
     8341 ccggagagcc agcgttttga cggaagccct gaccgggtat tgggaaactt tttgtactat
     8401 gaggcccagc cccatgaccc ggctgtgctg caggaggcgc tacggacgga gcagcacagc
     8461 cgggggcggg ccgtccccgg cgatttcaaa gtccacgtca ccgctttgca tgacagccgg
     8521 attgaaaccg aggcccggcg tattgtggac agcctgaaag atctgtccgc gcctaacagc
     8581 cccaataaaa cgcatttcat ggtggagatc tccccgcact ttgcggcgat tgcttccacc
     8641 aaggataatg accggctctt tgccatgctg ccctataaat ccctgtgttt taccgggatg
     8701 aaaggcaggc atggcgtgta tgccgtgatt aaccgggacg agcgccggga tgtgagtatt
     8761 cgcaggcccc ggccctctat ccgcaagcag ctatccgaca gcaaacaggc cgccagccca
     8821 aaaaaggcgg cggccagaac caaaaagaat gaattggagg tataaggaat gatgcacttt
     8881 accgtggagg aagaaaacct gatctgtatg taccacaatg cagaccggcg cagaacgata
     8941 aacaggctca ccgccgtcat gccggttatg gatggggata tgcgggcgca ggcccagcag
     9001 accctttcca agctggaacg catgacggat gccgactatg acggccagaa gttccacttc
     9061 acagacgaat aacgaacagc agacgccggg cgcggcggcc atgtggtcgc ttgcgtccgg
     9121 cgtcttttct ttttgcctat tcctacgcat ttagaacccg gtattgtcgg ggataggatg
     9181 aagtattcgc ttgcccctgc aaacatgcgg gagaagcctt gcagcgggcg gttttcaatc
     9241 ccttaaatgc gtaggttgca gctataatta gacccctgaa acagatatag ccgcgcagcg
     9301 ggtgtatctg tttcaggggt gtgcaggtga tagccgcaaa gcggcgtaag agggtgcagc
     9361 cctcttgccg tctatctctg ttccagcggg aagctcggca atgcttccag cagtttcagg
     9421 gaaatgcgct gccgtatatc ttcgtcaatg actttcttct ttttgccgtt tgcctgtctg
     9481 atctccaccg tggaaagctc gttgatgtag tcagcataat gggccaacac tttttccgtg
     9541 gcccacctct caccggcaac ggcggctttg atcgtttcat agtccagcag cttttcttgt
     9601 ccgtccatcc tgataccccc gtcagactga ttttttctat gataccatat tcggaagcat
     9661 ggaaccagag aaaacggcgc ttttctgctg gtaaattcta cgcatttaga acagcacttc
     9721 ggcggggata ggataaaaca cccacatacc ccgctgacca tgccggaaaa gccttgaaat
     9781 ataagggctt ttacagctct aagtgcgtag gtttccagta tcttttccgc tggcgctcgg
     9841 cctctttgcg gcggcgcatc cgtttggagc aggccgcgca gtatttggcc cggttagact
     9901 tcggcgtgaa cgtggccccg cactcacagc agcgtttcac ttcgtcccgg tctttggtga
     9961 tctcggcata cagggccgca tccagcggga ggacagcggc cttaaaccag cggcacagaa
    10021 gcgaatagga aatgctctgt acgcaaatac attcttcgcc gtcgtccagc aacaggcagt
    10081 tgccgtcaca atagttgcag cactcatgcg tcagcttccg ggccttgcga tattgagggt
    10141 aattcattcg tggaatactc atagctcttg cgtccattcc tttcccggcg tcatacgcaa
    10201 aatgccgtcc acattctgct tgatgatgcc gtattcctgc atctgctttt tcgcttcgcc
    10261 gtacttctca tacagccgtt ccttttcctc ggccaatcgc ttgtactcgg ctttcagggc
    10321 gtacagatcg gggagctttt tcacaccctg tccctttagg gcctttgccg cagcttcata
    10381 caaaatcaac gtgctttcat gctcacgccg gaattttgcc ttatccgctg cattgcggta
    10441 ttccatagcc acgggccaga gctgcttgta ggtagaaata ttctttatca gcaccgccat
    10501 atccgcaagc cggtgttcca tagctttgag ggtagcggcg gcctcgtcgc tggcggcggt
    10561 gatctcggcc actttgcttt ccaaatccgg gtaggtgtct atcccatgtt cagtcaggaa
    10621 attcaaagtc tttgcggcct gcttcaaatt gtggagcttc gcccacctct catagcctgc
    10681 cgactgctgg gccttaatgt tgttttccag atcaatccgc aaagaaatcc ccttgcgctc
    10741 tttgggcgct ctggtgataa cgcggccttt gatacgctct ttgatggctt cctcggtgta
    10801 ggcttccccc aatgtcttgc agcgggtaaa gcgttcctgt ccgggagcgc ggaaagaaat
    10861 aaactttccc ggcttgatct catagccctg cgcctgcatg agccgcagga aatcatcaaa
    10921 atcttttgcc tgcgggatag cggcgtcaat ggctgctttg agcttcgctt tccagctcgt
    10981 ccctttccgc tgggcgtccc actcggcata gctcttgccc ttactctggc cgggcttcac
    11041 cacggacaat ccgttttcct tgcacagcct gtcgctggtg cgccggatat aggcatagct
    11101 ctgtttgttg gaaatgtact tgcgctgatt taccatatcc acggcacaga aaataatgtg
    11161 attgtggacg tggcccttat caatatgggt agtcagcaca tagggatatt tcccttgcag
    11221 cacttcctcg gcaagctgct tcccgatctc atgggcctgt tccggcgtgg cttcgccggg
    11281 ggcaaaggat tgtatcaggt ggtggcccag attattgccc ttttggtatg cctgcgccag
    11341 cagcatttca aattcaatgt ccgctgtttc ataagagcag gcaaaggagg ccaccagcag
    11401 cttgccgtca gttttgtccg ggttctcgat gtagtcaagg gcttttttca gcgttgactt
    11461 gataggatgt atctttgtaa ctgccatatc tgcgccagcg ccccctttat atcctcgatg
    11521 tcctgtgcgt acaccggccc cataccgttc agcctctttg caatctgatt gacgttgacg
    11581 ccgatcttct gtatctcggc ggtctgcgcc cggatgtcgc tggtgtccat gttgacgata
    11641 tagccgtcaa tgagcatctt ccgggcgtag gctgaaaagt tcttcgtatg cagcagcgcc
    11701 attttctgtt caatcatagc ccgttcctcc ggcgtcacag ggacgcgcag cacgattttt
    11761 cgctttctgt ttgccatgct tcaccgtcct ttccattcag gggtttgggg atttccccaa
    11821 caagcaaaac ggacgcggcc cggcagggac ggggcaggcc gtttttccgg gagtggctac
    11881 actccctatg cttgctacgt tactgtttct ccactaacaa gacgtttcca aagccccaaa
    11941 ctacccgcaa aatagccctc tggaaagaaa aaagtgcaaa aaaatagaag ctacgctgtg
    12001 cagctcccgc taaaagtaaa acagaggatg ccagttacac atcctccgtt tcattcttct
    12061 tcggcttttc aattagtatg gcgctgtcaa tcgcgccctc gatgatcggc aggtattctt
    12121 cggggcagag tttcagcttg tggctgaccc tttgccgcag ttcgctttcc tcccgcatga
    12181 tctccgggtt aaagtagcgt tccacaggaa gcccgcaggc tttaataagc tgtatgacaa
    12241 ccggcagact gggaatggcc ccgtcgtttt cgatgttggc aagatagcgc cagtcaatat
    12301 ttattttctc tgccattgct ttgcgcgaaa cgcgctttgc ttgccgggcc gctttcacat
    12361 ccgcgccgaa agtttcaaag ccgggacaat cttcaatatt cgccatatcg cacctcccac
    12421 ttacattgta tatttcatac tctgcccgtg gaatgttggt atatgcacct ttcttaaagt
    12481 ttatatttca cattttgttt cttttattct tcgagcaaaa gaattataat gtaccctgtg
    12541 gaggtgtact atggactaca tgacgttgaa agaggcaggc gagaaatggg gtgtgccccc
    12601 acgatggata aactactatt gtgctgcggg acgcatcccc ggcgctgtaa agatggcgac
    12661 gatctggctc attcccaaaa ctgcggaaaa gccgattgac aggcgtacaa agaaaggaaa
    12721 ggcggttact gaatagtata atgaaaccgg cgcattttgg atatggtttt gaagagagag
    12781 aaagcatggt attttgtcac ggaaaaacta taagatgata gatggcaggc tgctccagac
    12841 cgataaaaag tattctcatc tgaaacagaa agaaaagatt tacacatgga tgtatgaaga
    12901 agcgaaactt tatcatgaca gaaatggaaa atgcccgaaa caaagacgag gaagctgctg
    12961 ctgtatcagc cgtatatggt agaattgaaa aggccggcat ctggattctt tatggtgaag
    13021 tctccagaca ttggataact gctggtctaa tgcgcctttg agtatggggt gattgatttt
    13081 aataccgggg aggtacggct ttttgaggaa aatattctgg gatttacatt tggcgattat
    13141 tacatttcct gtaactgcaa ggacacggat gatgaagata tgaggaatat agacttgctg
    13201 attttctact gtcttgaacg gtattaggac tgaatctgct gcgcggagtg cggcaagagc
    13261 gaccggaggg agtacacaca ggccctgcct gtgtgaaaag ccataccgca aggtgcattc
    13321 gcaaaaaaga ggccgcgcat tttgctgagg cttggggtgt ccccaacaag caaaacgaga
    13381 acgccgcagg cgggcaagtg gtggatcaga atatccgtaa aggatattct gatccattgc
    13441 ttgccagtat tatcgggatg ttttgacaac cgctgtaatc gaaacatccc aaacattaag
    13501 aaggtgttaa ttattattat cttgatttaa ttcgtttaaa ccaataatat agatacatac
    13561 accatgaaaa gaagcgagta tcccaatgac ggatgattaa aatccgaaaa gaaatcgctt
    13621 tgaaactaca gggaaactac agactgttat gttatcttct taaaatggag ggatttttat
    13681 gtcgatacga attctacttg tcgaggatga tgatcatatc tgtaatacag taagggcgtt
    13741 tctggctgag gcaggatatc aggtggatgc ctgcacagat ggaaatgagg catacaccaa
    13801 gttttacgaa aacacttatc aactggttat tcttgatatt atgctgcccg gtatgaacgg
    13861 gcatgaactt ttgcgtgaat ttcgtgcgaa aaatgatact cccattctga tgatgacagc
    13921 cctgtcggat gacgaaaacc aaatccgggc gtttgatgca gaggcagacg actatgtaac
    13981 aaagccattc aagatgcaga ttttactaaa gcgggtggaa gccctgctgc ggcgcagcgg
    14041 tgcgctggca aaggaaatcc gtgtcggcag gctgacactt ctgccggagg atttcacggt
    14101 actttgtgac ggtacagagc tgcccctgac acgaaaagaa tttgaaatcc ttttgctgct
    14161 ggtgcagaac aaaggcagaa ccttaacgca tgaaatcatt ttgtcacgta tatggggata
    14221 tgactttgag ggtgatggca gcacagtcca cactcatatc aaaaatttgc gggccaagct
    14281 gccggaaaat atcatcaaaa ccattcgcgg tgtaggatac cgattggagg aatcataata
    14341 atggaaagaa aagggatttt cattaaggtt ttttcctata cgatcattgt cctgctactg
    14401 gttgtcggtg taacagcaac actgtttgca cagcaatttg tgtcttattt cagagtgatg
    14461 gaattacagc aaacagtaaa atcctatcag ccattggtgg aactgattca gaatagcgat
    14521 aggcttgata ttcaagaggt ggcagggctg tttcactaca ataaccaatc ctttgagttt
    14581 tatattgaag ataaagaggg aagcgtactc tatgccacac caaatgccaa tacatcaaat
    14641 agttttagac ccgactttct ttatgtggta catagagatg ataatatttc gattgttgct
    14701 caaaacaagg caggtgtggg attgctttat caagggctga caattcgggg aattgttatg
    14761 attgcgataa tggttgtatt cagcctttta tgcgcgtata tctttgcgcg gcaaatgaca
    14821 acgccgatca aagccttagc ggacagtgcg aataaaatgg caaacctgaa agatgtaccg
    14881 ccgccgctgg agcgaaagga tgagcttggc gcactggctc atgatatgca ttccatgtat
    14941 gtcaggctga aagaaaccat cgcaaggctg gaggatgaaa tcgcaaggga acatgagttg
    15001 gaggaaacac agcgatattt ctttgcggca gcttctcatg agctaaaaac gcccatcgcg
    15061 gctacaagcg ttctgttgga gggaatgctt ggaaatatcg gtgactacaa agatcattct
    15121 aagtatctgc gcgaatgcat caaaatgatg gataggcagg gcaaaatcat ttccgaaata
    15181 ctggagcttg tcagcctgaa tgatgggaga atcgtaccca tagctgaacc gttggacata
    15241 gggcgcacgg ttgccgagtt gctgcccgat tttcaaacct tggcagaggc aaacaaccag
    15301 cggttcgtca cagatattcc agccgggcaa attgtcctgt ccgatccgag gctgctccaa
    15361 aaggcactat ccaatgtcat attgaatgca gttcagaaca cgccgcaggg aggcgaggta
    15421 cggatatgga gtgagcctgg tgctgaaaaa tgccgccttt ttgttttgaa catgggcgtt
    15481 cacattgatg atactgcgct tccaaggctg ttcaccccat tctatcgcat tgatcaggcg
    15541 cgaagcagaa aaagtgggcg aagcggttta ggacttgcca tcgtacaaaa aacgctggat
    15601 gccatgagcc tccagtatgc gctggaaaac acctcggatg gcgttttgtt ctggctggat
    15661 ttaccgctca catcaacatt gtaaatattt aaaacttaaa tgattttgac cgacaggtat
    15721 aaccctgccg gtcttttttt gctgctacag gaaaactaca gactggctac agggaaagta
    15781 cagatgcgct tgccataata acaatcgtac cagccacaaa tcgtagtttt attgcaaagg
    15841 aggcattcga tcaaatggaa aaaagcaact atcattccaa tgcggatcat cacaaacggc
    15901 atatgaaaca atccgtggaa aagcgggctt ttctatgtgc gttcattatt tcgttcacag
    15961 tctgcacgct gtttttgagg tggagactgg cttccgtatt ggaggcaaca cagataccgc
    16021 ccatccctgc aactcataca ggcagcagca ctgacgtagt ggagaatttg gaggaaaacg
    16081 ctcttgccac cgccaaagaa cagggagatg aacaggaatg gagcctgatt ttagtgaaca
    16141 ggcagaaccc catccccgca cagtacgatg tggagcttga gcaactatca aatggtgagc
    16201 ggatagatat tcggatttct ccctatcttc aagatttgtt tgatgccgca agaactgatg
    16261 gagtttaccc gattgtcgca tccggatacc gaacaacaga aaaacagcaa gaaattatgg
    16321 atgaaaaaat tgccgaatat aaggcgaaag gctacacctc tgcacaggct aaagcggaag
    16381 cagaaacttg ggtggccgtg ccgggaacga gcgagcatca gcttggtctt gctgtggata
    16441 tcaatgcgga cggaattcat tcaacaggca acgaggttta tagatggctg gatgaaaaca
    16501 gctatcgttt tggttttatt cgtcgctacc caccagacaa gacagagata accggtgtga
    16561 gcaacgagcc gtggcattac cgatatgttg gcatcgaagc tgccacagag atgtacaacc
    16621 aaggggtttg ccttgaggaa tatttaaaac cagaaaaatg agaaaaggat gttatgttat
    16681 ggacagaaaa agattgacac agcgtttccc gttcctgctt ccaatgagac gggcgcagag
    16741 aaaaatgtgc ttttatgcgg gaatgagatt tgacggctgt cgctatgcgc agacgatagg
    16801 ggaaaaatcg ctttcccatt tgctctttga aacggattgc gcattataca accacaatac
    16861 cggatttgac atgatatacc aggaaaacaa ggtgttcaac ttaaagctgg cagcaaagac
    16921 cttaaacggc ttattgataa gaccggggga aaccttttct ttctggtggc tggtacgcca
    16981 tgcggacaaa gataccccct ataaagacgg ccttacggtg accaatggta aactcaccac
    17041 catgtcgggc ggcggtatgt gccagatgag caatttacta ttttggatgt tcctgcatac
    17101 gccattgaca attatccagc gcagaggtca cgaagtaaag gagtttccag agccaaacag
    17161 tgacgagatt aaaggggtgg atgcaaccat tccagagggc tggattgatt taaaagtgcg
    17221 aaacgatacc gactgcacct accaaatatg ggtgacctta gatgatgaga aaatcatcgg
    17281 tcaggtgtcc gccgacaaag agccccaagc attatacaaa attacaaatg gcagtatcca
    17341 gtatgtccgt gaaagtggcg ggatttatga atatgcccag gttaaacgga tgcaagttgc
    17401 cttaggtacc ggggaaataa tagattgcaa gctgctttat acaaacaaat gcaaaatctg
    17461 ttatcccctc ccggaaagtg tggatattca ggaggagaac caatgagaaa aagtgtgggc
    17521 attactgttt ttggatgcga acaggatgag gcaaatgctt tccgcgcttt atcgccggat
    17581 tttcatatta tccctacgct gattagcgat gcgatatcgg cagacaacgc aaaattggcc
    17641 gctggcaatc aatgcgttag cgtaggccat aagtccgagg tttccgaggc gacaattctt
    17701 gcgctgagaa aggtcggggt aaaatacatt tctacccgca gcatcggctg cgatcacatt
    17761 gatacgactg ccgccgagag aatgggaatc tcggttggca cggttgcgta ttcgccggac
    17821 agcgttgcgg attatgcttt gatgctgatg ctgatggcca tacggggtgc aaaacccacc
    17881 atgcacgccg tggcgcaaca agatttcaga ttggatcgta tccgggggaa agaactgggg
    17941 gatatgactg tgggagttat tggaaccggc catatcgggc aagcggtcgt caaaaggctg
    18001 cggggatttg gatgccatgt gctggcctat gataacagcc gaaaaatgga tgcagattat
    18061 gtccagcttg atgagcttct aaaaaacagc gatattgtta cgctccatgt gccgctttgt
    18121 gcggataccc gccatctgat cggtcagaag caaattggag agatgaagca aggcgcattt
    18181 ttaatcaaca ccgcgcgcgg agcgcttgtc gataccgggg cgctggtgga ggcgttggaa
    18241 agcggaaagc tgggtggtgc ggcactggat gtgttggagg gcgaggatca gtttgtttat
    18301 accgattgct cgcagaaagt gcttgaccat ccctttttgt cgcagctttt aaggatgccg
    18361 aatgtgatca tcacacccca tacggcgtac tacactgagc gtgtgctgca ggatactaca
    18421 gaaaaaacaa tcaggaattg tctcaatttt gaaaggagtt tacagcatga atagaataaa
    18481 agtcgcaatc atcttcggcg gttgctcgga ggaacatgat gtgtcggtaa aatccgcaat
    18541 agaaattgct gcgaacattg atacggaaaa attcgatccg cactacatcg gaattacaaa
    18601 aaacggtgta tggaagctat gcaagaagcc atgtacggaa tgggaagccg acagtctccc
    18661 cgccatactc tccccggata ggaaaacgca tgggctgctt gtcatgaaag aaagcgaata
    18721 cgaaacacgg cgtattgatg tggctttccc ggttttgcat ggcaaattcg gggaggatgg
    18781 tgcgatacag gggctgtttg tattgtctgg tatcccctat gtgggctgtg atattcaaag
    18841 ctccgcagct tgcatggaca aatcactggc ctacattctt acaaaaaatg cgggcatcgc
    18901 cgttcccgaa tttcaaatta ttgataaagg tgacaagccg gaggcgggtg cgcttaccta
    18961 ccctgtcttt gtgaagccgg cacggtcagg ttcgtccttt ggcgtaacca aagtaaacgg
    19021 tacggaagaa cttaacgctg cgatagaagc ggcaggacaa tatgatggaa aaatcttaat
    19081 tgagcaagcg atttcgggct gtgaggtcgg gtgtgcggtc atggggaacg aggatgattt
    19141 gattgtcggc gaagtggatc aaatccggct gagccacggt atcttccgca tccatcagga
    19201 aaacgagccg gaaaaaggct cagaaaatgc gatgattaca gttcccgcag acattccggt
    19261 cgaggaacga aatcgggtgc aggaaacggc aaagaaagta tatcgggtgc ttggatgcag
    19321 agggcttgcc cgtgttgatc tttttttgca ggaggatggc ggcatcgttc taaatgaggt
    19381 caataccatg cccggtttta catcgtacag ccgctaccca cgtatggtgg ccgccgcagg
    19441 aatcacgctt cctgcactga ttgacagcct gattacattg gcgttaaaga ggtgataagt
    19501 atggaaaatg gttttttgtt tttagatgaa atgttgcatg gcgtccgttg ggatgccaag
    19561 tacgccacat gggataactt cacaggaaaa ccggtggatg ggtatgaggt gaatcgcatc
    19621 atcggcacaa aggccgtggc gtttgctctg cgcgaggcac aaatccatgc ggctgcgctt
    19681 ggctatggct tgcttttatg ggatggatat cggccaagaa ctgcggtgga ctgcttcctg
    19741 cgttgggcag cgcaaccgga ggacaatctc acaaaagaaa aattttaccc caatatagag
    19801 cgagccgagt tgattacaaa gggttatgtg gcctcacaat ccagccatag ccgtggaagc
    19861 gcaattgatc ttacgctcta ccacctggat acaggggaac ttgtttcaat gggaagtaac
    19921 ttcgatttta tggacgaacg gtcgcatcat acagcaaaag ggatagggga tgcagaggca
    19981 caaaatcgaa gatgcttgcg taaaatcatg gaaagcagcg gatttcagtc ttatcgcttt
    20041 gaatggtggc actataagtt gattgatgag ccataccccg atacctattt taattttgcc
    20101 gtttcataat gaaagtattg gttttgtatc agattttgga tttgataaaa tctatgctgg
    20161 tcatggacaa tcttttactg gaaagtggtt aaagcatctt ttgtaataca ttgcaaatta
    20221 tagtattcaa tctgcccagc ggcagaaaag aaaaaaaccg ctgttgggca gacctttccc
    20281 acgcccattt tgtattttgc tggcggcggt tttgagaaat caaggccgcc gttcttttct
    20341 ggcagcaaaa ggtgtggcgg cccataggag gatgccgcta tgctgttttg ctattgccat
    20401 gctctaatcc acttccacca ctcaaaaaag ccttgcccct ccacagggct attccggtta
    20461 tgagtatgta ctataccatg taaatcagca gcatagcaaa gcattttctc gcccggtagg
    20521 tttgcgacct gccgggcgat ttttgtcgtc ttttgcggcc cggctgtccc caaaaaaatt
    20581 ttttgagaaa ttttcaaaaa ggaggtattt agcgggtagc tggcggcctt gtcaaagtct
    20641 tgttagcgga aagggagaga accacctaag tcccccacag aaagggggtg agaagatgga
    20701 tgctatcccc cgcgactatg aagcgcgg
//
LOCUS       DADXTG010000353         7073 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.120, whole genome shotgun sequence.
ACCESSION   DADXTG010000353 DADXTG010000000
VERSION     DADXTG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.120"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            100..1602
                     /gene="eat(A)"
                     /locus_tag="IWI57_002926"
     CDS             100..1602
                     /gene="eat(A)"
                     /locus_tag="IWI57_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBC2450987.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1630..2196
                     /locus_tag="IWI57_002927"
     CDS             1630..2196
                     /locus_tag="IWI57_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBC2450988.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            2407..3468
                     /locus_tag="IWI57_002928"
     CDS             2407..3468
                     /locus_tag="IWI57_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBC2450989.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3471..4367
                     /gene="murQ"
                     /locus_tag="IWI57_002929"
     CDS             3471..4367
                     /gene="murQ"
                     /locus_tag="IWI57_002929"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBC2450990.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4381..6363
                     /locus_tag="IWI57_002930"
     CDS             4381..6363
                     /locus_tag="IWI57_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBC2450991.1"
                     /translation="MAKNELTLEELAKHIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6428..6874)
                     /gene="nrdI"
                     /locus_tag="IWI57_002931"
     CDS             complement(6428..6874)
                     /gene="nrdI"
                     /locus_tag="IWI57_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBC2450992.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         2343 a         1235 c         1564 g         1931 t
ORIGIN      
        1 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
       61 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
      121 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
      181 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
      241 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
      301 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
      361 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
      421 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
      481 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
      541 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
      601 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
      661 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
      721 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
      781 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
      841 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
      901 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
      961 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     1021 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     1081 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     1141 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     1201 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     1261 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     1321 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     1381 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     1441 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     1501 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     1561 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     1621 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     1681 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     1741 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     1801 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     1861 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     1921 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     1981 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     2041 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg ccataatgcc
     2101 ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt ctttacattc
     2161 atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt tatttaaata
     2221 aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg acaattcttt
     2281 tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa agtattgaaa
     2341 ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca gaaaaagagg
     2401 gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga gaagaaacag
     2461 tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat gcatatacca
     2521 gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag
     2581 aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc gggtttttca
     2641 ttagaagatc taacgccttt gaaagcaatt ggtgtaacag gtttaagaat ggattatcat
     2701 atttccaatc agcagattgc cgactggtca catcaattga aaatcagttt gaatgctagc
     2761 acgatcacgc ctaaagatat cgatgaattg aaagaagcag aagctgattt ctcacagatt
     2821 gaagcttggc ataattatta tccacgtccc gaaacagggc tggacaaaga atggtatcag
     2881 aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc aaggttttgt accaggagat
     2941 acagagttga gaggcccact atatcaagga ttgccaactt tggaagaaca tcgagggatc
     3001 catcctcttg caggcgctct agatctatta gcaagtaaca cggatattgt ttacatcgga
     3061 gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt ctttccaaaa agaacagaca
     3121 gtcttgctcc atacggaacc tgtagatgaa gaattttacg aatatatatt agggaagcat
     3181 acgaatcggc aagacgatgc aagagatgtc atacgaagcg tagatgcacg ttttcgagag
     3241 attccgccta tcccagcaag aaatacagct acgcgtatga aagggtctat tactttagat
     3301 aacgaaaagt atttgcgcta tatgggtgag atccaattaa ccaaatatga tttgccagcg
     3361 gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag agctaccatt gatcaaccaa
     3421 atcaaagctg gtatgaacta tcaatttata agaaaagaag gaagataaaa atgagcaaaa
     3481 tcaatttaga gaacctaaca acggaacgca gaaaccaaga aacttttgga ttagatgaga
     3541 tgagtatttc tcaagccgtt caactaatga acaaagaaga tcagaaagtg gccgctgcgg
     3601 ttgaaaaaga actgacaagt atcgaaccag tgattgctaa cacgattgag tcctttaaaa
     3661 aaggtggacg tctgatttat atgggagccg gaacaagtgg aagactaggt gtgttagatg
     3721 ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat ggtcataggt ctaattgctg
     3781 gtggagaaga agcaatgact gtagctgtcg aaggggccga agattcttat gagctagggc
     3841 aagaagatct aatcaagctg aatttgacaa aaaatgatat agttattggt atcgcagcta
     3901 gcggtcgtac accatatgtg atcggtggat tagattacgc aaaatcgatc ggtgctgtca
     3961 caggaacgat ttcttgcaat aaacaagccg aaatcagtaa acacgcggat ttcccaatcg
     4021 aagtcgatgc aggtccagaa tttttgacag gctctactcg attgaagtca ggaacagcac
     4081 aaaaacttat cttgaatatg atttctacta tcagtatgat tggtataggt aaagtattca
     4141 ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact aatcgaacgc tcaaaacgaa
     4201 tcatcatgca agcgacagaa gctgattacg aaacagctga aaagtacttt gaagaagcag
     4261 atcaaaatgt taagctggct atcgtcatga ttttgacaaa tagtgataaa gaggaagctc
     4321 aagcaaaatt agctgaagca aacggatttg taaaagggac cttatgaggg ggaaaagacc
     4381 atggcaaaaa acgaactgac tttggaagaa ctggcaaaac acatttataa aggggcaggc
     4441 ggcatgggca atgtagaaag cattgtccac tgcatgactc gtgtgcgtat gaagatcatc
     4501 aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc caggagtgct tggtgtagta
     4561 gaagatgaac agcttcaagt catcatcgga cctgggaaag tgaacaaagt agcacagcaa
     4621 atggttgatt tggcaggcgt gaaattagga gaaaaacttt cttctgtaag tacagctaca
     4681 gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac gggcacaaga aatgaaagcg
     4741 gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg tattaaaaga catttctaat
     4801 atctttgtgc caatgattcc tgcatttgtc ggtactggga tcgtggcagg gattgccgca
     4861 gttttagcaa acttagtcac agccggaaca cttgatgcag caacatggca acaatatatc
     4921 gatgtgatga acatcttgaa aaacgggatg tttagctacc tagttatcta tacaggtatc
     4981 aatgctgcac aagtttttgg tgcaacacca actcttggtg gagttatcgg ggccgttgtg
     5041 atgctgacag ggatgaatcc ggaagcacca ctgaaaaact tatttactgg tgaagctctt
     5101 gcagctggac aaggtgggat cctaggtgtc atcttcgctg tttggctatt gtcaatcgtt
     5161 gagaaaaaac tgcataaagt agtacctgat gcggtagata tcatcgttac accaactctt
     5221 tcattgattg ccatcggtct gattgaaatt ttcttcatca tgcctttagc aggtttcatt
     5281 tctgatggca tggtcggtgg gatcaactgg gtacttggcg ttggtggagc atttgccggc
     5341 ttcgttctag gcacattatt cttgccgatg gttatgttcg gactgcatca aattttgact
     5401 ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc tattgcttcc aattcttgcg
     5461 atggctggtg caggacaagt gggtgcagca ttggcattat ggattcgctg caaaaaagat
     5521 aaagaattaa cagaaatgat caaaggagct ttaccagtcg ggatcctagg aatcggcgaa
     5581 ccattgattt acggagtaac attacctcta ggacgcccat ttattactgc ttgtatcggt
     5641 ggaggtatcg gcggggcagt gatcggaatg atcggaaatg tcggggctat cgctattgga
     5701 ccttcaggag ccgctttgat cccattgatc tctgacggta aatggttagg ctacatcctt
     5761 ggattattgg cagcttatgc tggtggcttc gttgctacat tcttcttcgg tattcctaaa
     5821 gaccgtttag aaaaagaaga attagcgact gaaacagcca cagtaaatga agtgcctgaa
     5881 aaagtagtac ctcaaacttc agctgaaagt gtgactatcc tctcatcagt tgctgacgga
     5941 acagttgttt ctttagataa agcaagtgat ccagtttttg cacaaaagat gatgggtgaa
     6001 ggctactttg tagaaccaga aaacggacat atctattctc cagttactgg ggtagtcagc
     6061 tctgtcttcc caacgaaaca cgctattggg atcacaacgc caaatggttt agaaatcttg
     6121 cttcatatgg gaatcaacac agttgactta ggcggtacgc catttgatgt gaaagtaaca
     6181 gaagggcagc aagtgacacc tgatacgctt gtagcagaag ctgacttaga agcgatcaaa
     6241 gcagcaggca aagaaacatc catgatcgtc ttggtaacca atatggatcg tgtaaaaaac
     6301 tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac ctgtgatgga tgtcaaagct
     6361 taatatgata gaaacaatag aaacgaaaga agaaactgaa atctattttt cagcttcttc
     6421 ttttttatta tttattgttt tctgctaaaa tctgataaat tcgtgtcaca tcagatggtg
     6481 ttccccgtaa ttcataatct gccaagaaag gaaagccaaa tgtttgtgca tattgtttgg
     6541 ctgttagaca atactgatta ttgaagtttt tatttccgct accgactaca ccaagacaaa
     6601 gtttatgatt gtcttcatgt tccagatatt cacgcatcgt ttcggtcaag atttctgtat
     6661 caccgttatc taaaccattt ccgccgtcta aataagtagg aacgaaagta aaaaatggtt
     6721 ctgtttcttt cgtaaaatca ctattttcat ggatttcttt taaagtaact tcaggattga
     6781 caggatcttc tgcatgcatt ttttcagcat attctgctag atgtttggca aaggcacgcg
     6841 tatttcctga aatcgatata tataaaatgt tcatggatca tcctccaatt ctttcctttt
     6901 agtatagcat cttttttaag ttttttggtg gtgtaatttg gaaagggact aggcgctttg
     6961 cttgtataat gagaaaaaag ccggtagaat tatatttcta ttaaagtttt tattaattaa
     7021 gtaaaaataa aaaaggtttg ttatactttt tgataaaagc ggttttattt tgg
//
LOCUS       DADXTG010000354         6012 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.132, whole genome shotgun sequence.
ACCESSION   DADXTG010000354 DADXTG010000000
VERSION     DADXTG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.132"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..475
                     /gene="lsa(E)"
                     /locus_tag="IWI57_002932"
                     /gene_synonym="lsaE"
     CDS             <1..475
                     /gene="lsa(E)"
                     /locus_tag="IWI57_002932"
                     /gene_synonym="lsaE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Lsa(E)"
                     /protein_id="HBC2450993.1"
                     /translation="VAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDT
                     SYLKGNLSEFAYNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCE
                     SAHLYIWDEPLNYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            529..1332
                     /gene="lnu(B)"
                     /locus_tag="IWI57_002933"
     CDS             529..1332
                     /gene="lnu(B)"
                     /locus_tag="IWI57_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HBC2450994.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            2020..2841
                     /locus_tag="IWI57_002934"
                     /pseudo
     CDS             2020..2841
                     /locus_tag="IWI57_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            2984..3208
                     /locus_tag="IWI57_002935"
     CDS             2984..3208
                     /locus_tag="IWI57_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBC2450995.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            3223..4092
                     /locus_tag="IWI57_002936"
     CDS             3223..4092
                     /locus_tag="IWI57_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBC2450996.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            4073..4807
                     /locus_tag="IWI57_002937"
     CDS             4073..4807
                     /locus_tag="IWI57_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBC2450997.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4840..5748
                     /locus_tag="IWI57_002938"
     CDS             4840..5748
                     /locus_tag="IWI57_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBC2450998.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5745..5993
                     /locus_tag="IWI57_002939"
     CDS             5745..5993
                     /locus_tag="IWI57_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HBC2450999.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         2078 a          869 c         1273 g         1792 t
ORIGIN      
        1 agttgccatt atcggaaaaa atgggagtgg taagtctagt atcctaaaat tgattaatgg
       61 agatgatatt aaatttaccg gaaattttat gctagcaagt ggactaaaaa tttcttatat
      121 ttcgcaagat acttcatatt taaaaggtaa tctatctgaa tttgcctata ataataagat
      181 cgatgaaact ctatttaaaa cgattcttcg taaactggat tttaatagag agcagtttga
      241 taagaacatg gtggatttta gtgctggtca gaaaaagaaa gtactaattg ctaaaagcct
      301 ttgtgaaagt gcacatttgt atatatggga tgagccattg aactatattg atattttttc
      361 acgtatccaa attgaaaaaa tgattttgga atattgtcct acactattgt ttgtggagca
      421 tgatgatgct ttttgcaata acatttgtac gaaaaatatt aatttaggtt tgtagagatt
      481 ttgagttgcc acaataacta aaagatattt acatgaaagg gtgaagaaat gttaaaacaa
      541 aaagaattaa ttgcaaacgt taagaatctt actgagtcag atgaacgaat tacagcttgt
      601 atgatgtatg gatcgtttac caaaggagaa ggtgaccaat actctgatat agagttctat
      661 atatttttga aagatagtat aacctcgaac tttgattcat ccaactggtt gtttgacgta
      721 gctccgtact tgatgcttta taaaaatgag tacggaacag aggtagttat ttttgataat
      781 cttatacgtg gggaatttca tttcctttct gaaaaagata tgaacataat cccctcgttt
      841 aaagattcag gttatattcc tgatacgaag gctatgctta tttacgatga aacagggcaa
      901 ttagaaaatt atttatcaga gataagtggt gcaagaccaa atagacttac tgaagaaaat
      961 gctaattttt tgttgtgtaa tttctctaat ctatggttga tgggaatcaa cgttctaaaa
     1021 agaggagaat atgctcgttc attagaactc ttatcacaac ttcaaaaaaa tacactacaa
     1081 cttatacgta tggcagaaaa aaatgctgat aattggctaa acatgagtaa aaaccttgaa
     1141 aaagaaatta gccttgaaaa ttataaaaaa tttgcaaaga ccactgctcg attagataag
     1201 gtagaattat ttgaagccta taaaaattct ttgctattag ttatggattt gcaaagtcac
     1261 cttattgaac aatacaactt aaaagttaca catgacattt tagaaagatt gttgaattac
     1321 attagtgaat aggagagtaa cgttattcac agtattgggt actgaaaatt aacagcatac
     1381 ctcagttttt caaaaaacag cgaagcctat ggatatgttc ccgtctaccg atagtgcaaa
     1441 aagtgtcgtg gatatgtttc cgagaacgga catacacaaa tgacattcat tctgtcctct
     1501 caatccatgt ggaagcagta tgtctgttaa caagaaaata attattgttc gtcctgacga
     1561 gttgttagac tcgaaaagga taaacatcat ttttagaacc ggtagccgat ttttggctag
     1621 cggttttttg ggctctttgg caaatagtgg tgattgaatc tcgcgctatc ctatggaaaa
     1681 ccgccttgtg tgaggggctg cgagaccgct tcgatcctag aattgctgaa gcacgaatct
     1741 agcgtcactc acatttcatc ttttgacgta gctgagacaa ggtgtttttc ttcgatccgc
     1801 tagtggttaa tctctttttg gtgcaaatat ggtttagaat ttctttgcat ggcttaaggg
     1861 agtggagaat cggatattag taggatttac atccacagat aaaaataggc ttgattttaa
     1921 cagcgaaact aaaataccct acttaaatga agtgatagat tagatgttgg gttgttagga
     1981 gatgcttaaa aatcctatgc aatcaatagt ttttagtgtt tgttgacttg atccctaaat
     2041 cgcctgagta ctaaacgttg agtgtgtctg cttactgtca ctagccagta agtgagactg
     2101 tagttaatct ctcattttcc ccagtattat gttgaaaata gccatgaagc gattatttct
     2161 aaagagttat ttttgcaggc tcaagaagaa atttatcgga gaagtaatat ctacacagga
     2221 gaaggcaaga acaaaagaat ttatagtagc aagtacgctt taagtgccat caccttctgt
     2281 ggatattgtg gcgatattta taggagaacc tattggaata ttcatggcag aaaagaattt
     2341 gtctggcgat gcgtgactag aatcgagcag ggtcccgagg tatgtaagaa ccgaaccgta
     2401 aaagaagatg aactctatgg tgcggtaatg accgcaatta ataagctact tgctggtggc
     2461 aacaatatga taaagacact ggaagaaaat attcatgctg tgattggaga aaccacagaa
     2521 taccaaattt cagagattaa taccctacta gatgaaaaac aaaaagaact aatcaagctg
     2581 gccaataaag gtcaagatta tgaatacctg gtagatgaga tcgatgagat gcgagataag
     2641 cgtcagactc ttttagtaga agatgcctcc ctcagtggcg agaacgagcg catcaatgag
     2701 ttgattgaat ttatccgcaa gaacaaattc cgcaccttag aatatgatga taagcttgta
     2761 aggaagataa tccagaacgt taaggtctat gaagaccact tcgtcatagc cttcaaacca
     2821 ggtatcgaaa tggaaatatg aaaaccagaa gtaaatagcc catgactctg aagtagagtt
     2881 gtgggttttc ttttaattgc tgattataat aatattttaa tgactattgc atttatcaac
     2941 caaatggttt atgttattct tagcataaac ttggaggtgc tgtatgacta cggcagaaat
     3001 gattaaagaa ctgtgtgagc aaatgaatat aagtgtttcc gaacttgcta gacgtattgg
     3061 ccagactcca cagaatttta ataaaaaatt acaacgagaa acagtaacct tggatgagtt
     3121 gaaggccatt gctgatgtgc taggtgtcaa gtttgtgcag gcatttattt taccagatgg
     3181 agatgaaata aaaatatcta acgaataaag gaggatgttt atatggtagg taatgttatt
     3241 caaatagtaa cagagaaatt atcatctttg ccttttatag aaggcattgt attagggggc
     3301 tcacgtgcaa gaagcaccca tacagaaaat tctgatatag atatcggaat ctattacaat
     3361 tcagactcat ttgacctgac agcgattaat caaattgcta cagagttgga tgatgagaat
     3421 agaaacaacc ttgttgtacc tcccggagcg tggggtgatt gggttaatgg cggtggatgg
     3481 ttagttataa acgggtgtca tgttgacttg atattacgtg atataaaacg ggtggaacaa
     3541 ataatcaaag atacggagca aggaattgtt actgccaatt atcagaccgg gcatccccat
     3601 ggttatataa gtgctatgta tcgtggagaa ttagcgatta gtaaaatact atatgctaag
     3661 aacgaaagct tatgcgaatt aaaaaagcag gcagaaattt accctactgc tctaaagaaa
     3721 agcttgatga acttttttat atttgaagct gaattctctt taatgtttgt aaaagcaaat
     3781 gcgggggcag aggataaata ttatattgca ggccatgttt ttcgtattat ttcatgctta
     3841 aatcaagtac tatttgcatg taataatgct tattgcatta acgaaaagaa agctataaaa
     3901 ctgcttgaaa cttttgaata taaacctgaa aaatatgccg aaagagttaa tcatattttt
     3961 gaagtactcg gtctttctct ttttgaatgc tacgacatga ccgagaaact ttataaagaa
     4021 gtgaatgaaa ttgtatcgga gataaataac tttttaaacg aggagagttc agatgaaaga
     4081 aaacaaatat gatgataata tattttttca aaaatacagt caaatgagtc gctcgcagaa
     4141 aggactggct ggtgcgggag aatgggagac tttgaaaaag atgctacctg attttaaggg
     4201 taagcgtgtg cttgatttag gatgcggcta tggatggcac tgtatatatg cgatggaaaa
     4261 cggtgcttcc tctgtagtag gtgttgatat ttctcataaa atgctcgaag tagcaaaagg
     4321 aaaaacccat tttccacaga ttgaatatga atgctgtgcc atagaagatg tggatttccc
     4381 agaggagagc tttgatgtaa tactaagttc gcttgcgttt cattatgtag cagactatga
     4441 gaatttaata aaaaagatat ataggatgct gaaggctggt ggcaatttag tttttacagt
     4501 tgaacatcct gtttttactg ctcatggaac acaagactgg tattataacg aaaaaggaga
     4561 aatactgcat ttcccggtgg acaattatta ttatgagggc aaacggacag ctatgttttt
     4621 ggaagaaaag gttacaaaat atcatagaac actgaccaca tatctaaata cactgctttc
     4681 aaatagtttt ataataaatc agattgtgga gccacagccg ccagagaaca tgatggatat
     4741 tccggggatg gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa
     4801 gatgtaataa tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg
     4861 atggatttag tactttcttt agcagaacag gatgaacgta ttcgaattgt gacccttgag
     4921 gggtcacgcg caaatattaa tatacctaaa gatgaatttc aggattatga tattacatat
     4981 tttgtaagtg atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat
     5041 ataataatga tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt
     5101 tcctatctta tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa
     5161 gagttagata attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt
     5221 agaattaaaa gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca
     5281 agggagtatg atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga
     5341 ttgtgccgta aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag
     5401 ctgctgagaa tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc
     5461 aagaactata agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc
     5521 acctaccgga tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg
     5581 ttcagggcgg tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat
     5641 aggaatataa caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc
     5701 ctggatagca catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga
     5761 aagcagggca cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga
     5821 ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat
     5881 atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat
     5941 atattgacaa tactgataag ataatatatc ttttaacccg tggtgctagt taattttaat
     6001 atcctaaaga at
//
LOCUS       DADXTG010000355         3311 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.187, whole genome shotgun sequence.
ACCESSION   DADXTG010000355 DADXTG010000000
VERSION     DADXTG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.187"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            361..825
                     /locus_tag="IWI57_002940"
     CDS             361..825
                     /locus_tag="IWI57_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBC2451000.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            809..1438
                     /locus_tag="IWI57_002941"
     CDS             809..1438
                     /locus_tag="IWI57_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBC2451001.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1792..3270
                     /gene="msr(C)"
                     /locus_tag="IWI57_002942"
     CDS             1792..3270
                     /gene="msr(C)"
                     /locus_tag="IWI57_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBC2451002.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         1143 a          541 c          677 g          950 t
ORIGIN      
        1 aaaaaggttg tgacaacatc ttgtcataac ttttttatta ctgtttaagc atactgatcc
       61 aatttctaat attcgacggg attcttgcaa aaggcgattt cggaaatttt tggcggtgta
      121 aaattatcta ctgcagaaga gctatcttct atagtttgat aagaatcaat aatcttggtt
      181 ttagcgttgt gctttcttta tcattttttc cgttcattct acaaaaaatt tcttttctct
      241 atacttttct attgagagcg ttttttttat tacacagctg attctttgac ttttttcttt
      301 ttttattcta ttattgacaa gtcaatcgtt gacgagtcaa ttattagaaa ggaggaaatt
      361 atgtctttag aagagattag tcacctttta tatcaaatca aattagccga tcaaaaaatc
      421 gtccagatct ttgaagaaaa tatcggtata agtcttacta gatacgagat catgatgaga
      481 ctgaaagaag aaaatccatt gctacaaagc agtttgcaag aaaagttgca gattgatcaa
      541 ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc gcgtcagaga
      601 aatcctcaga acaaccggga agtgtatgta gcgctaacag aaaaagccca gaagaaaatc
      661 aagcattgtg aaatggacca taaagataca ggttccgtgc tatccccggc tttcagcgat
      721 gtagaaatga aacaatttct aacactttta gataaattga atacccaact ttgcaatata
      781 acaacagaaa cgaaagaggt taataataat ggaacaaaaa aatgattttt caacaattat
      841 gactggtaga agatccgttc gcgtatatga tgaaacatac aaaatcccac acgaagaaat
      901 gctggaaatg atcaaagaag cgacgactgc accttcttct gtcaatatga aaccatggcg
      961 ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga tccgtttcaa
     1021 tacgagacaa aatgatactt cttctgcaat ggtcatgatt tttggcgata tgcaatgtta
     1081 cgaatatggc gaagaaatct acaaccaagc ttacgaatca gggaagatgc caaaagaagt
     1141 cagagatcaa caattagctg cgatcattcc ttattacaag agtttttcaa gagaagaaat
     1201 gaacgatgta gtcaaagtcg attctagctt agcggctatg caattcatgt tagttgcgcg
     1261 tgatcatggc tatgaaacaa atccaattgg tggattcgaa gcagatcagt tggcagaggc
     1321 atttggtttg gataaagacc gttatgttcc agttattatt ttatcaatag gaaaagcggt
     1381 agaagaaggt tacgagtctg ttcgtttagc accagaaaag atcactatgt tcgaataaaa
     1441 ggttttcagt tactattcag tagctattgc tttttttgaa tcgtgtgtgg taacctaata
     1501 aaaaaggagg tcggaaaaat gactgcatcg atgaaattac gtttcgaact tttgaataac
     1561 aactaaataa attcaaaaga catgtataaa caacaatttc atggatggag tatgtccatt
     1621 ttttcgaacg acagtagaat aggtgaaagc tatgacagat ttggtgattt agtcatagtt
     1681 atttcgttca gagtaggaaa agatgtgatg cagttttttt gcaaacagat ttttctgctc
     1741 ttttttagta tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat
     1801 ttagcagtaa atataacaaa tctacaagtc agctttggca accagctaga actatctatt
     1861 gattctcttc gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt
     1921 aaatcgactt tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa
     1981 acagagatca ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta
     2041 aatttggaat tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg
     2101 agcggagggg aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg
     2161 atgcttctag acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa
     2221 gagcttcgtt attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat
     2281 gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat
     2341 tatagtgcct atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag
     2401 tcgatcatga aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg
     2461 gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagact ggatcggttg
     2521 tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta
     2581 gagagaagac tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt
     2641 tttccagtac caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc
     2701 caattggaaa gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt
     2761 aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc
     2821 cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa
     2881 aagggttatc aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag
     2941 ttttcagaat cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg
     3001 acgaaaccgt tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt
     3061 tttactaagc caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca
     3121 acgatcgaag ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct
     3181 catgattctt actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa
     3241 ataaaaaaag tacttacgag aaatttttaa atgtgctacg atgattttcg tgacaaattt
     3301 aaaggagtgg t
//
LOCUS       DADXTG010000356         3094 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.197, whole genome shotgun sequence.
ACCESSION   DADXTG010000356 DADXTG010000000
VERSION     DADXTG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.197"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1336
                     /locus_tag="IWI57_002943"
     CDS             <1..1336
                     /locus_tag="IWI57_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBC2451003.1"
                     /translation="LDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRYFLDRVANRI
                     FELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRAGVQARGTKQ
                     QARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQTLLEHLDLL
                     IQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQENEEMAPDKR
                     MIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKRRLFLLKLLI
                     QQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVADKLLVFQGNG
                     KQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEWETIEDEIAE
                     LEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEWVED"
     gene            1348..2295
                     /locus_tag="IWI57_002944"
     CDS             1348..2295
                     /locus_tag="IWI57_002944"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBC2451004.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2315..2842
                     /locus_tag="IWI57_002945"
     CDS             2315..2842
                     /locus_tag="IWI57_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBC2451005.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(2905..>3094)
                     /locus_tag="IWI57_002946"
     CDS             complement(2905..>3094)
                     /locus_tag="IWI57_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBC2451006.1"
                     /translation="AAVPLYHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWH
                     LFVMLGAGFMYFSVLFFT"
BASE COUNT         1083 a          537 c          686 g          788 t
ORIGIN      
        1 actagacgaa ccaacaaacc atttagatta tgaagcgatc gagtggttgg aaaactattt
       61 gaaacaatac cgtggcgcac tattgatggt tacccacgat cgttattttc tagatagagt
      121 agcaaaccga atcttcgaac tttcatttgg aaaattatac gagtataaag gaaattatga
      181 agcttacgtc ttagaaaaag cagaacgtga tcgtgtagaa gtagaacaag aagaaaagcg
      241 caagcggctt tataaacagg aattagcttg gatgagggct ggcgtacagg cacgtggcac
      301 caagcagcaa gcacggatca accgtttcga agatctaaag gagaacttgt accaagtaaa
      361 ccaagaagat gatttagagc tgaacctagc gacacaacgt ttagggaaaa aagtactgga
      421 aatcaaagat ggcagttacc gtataaatga ccaaacactg ctagaacatt tagatttatt
      481 gattcaatct cgggaaagac taggtattac tggtaaaaat ggtgcgggaa aatcgacatt
      541 gcttaatatt ctagctggca gaattcccct ggatagcggt acaatgtcta tcggtgaaac
      601 ggttcatctt gcgtattata cacaagaaaa tgaagaaatg gcaccagata aacggatgat
      661 tgcttactta caagaagctg ctgaagaagc aaaaacagca gatggcagtc aaatcggagt
      721 agcagaatta cttgaacgat ttttatttcc aagatttatg catgggacac tgataggcaa
      781 actttcaggc ggagaaaaac gccggttatt tttgttgaaa ctgctgattc agcaacctaa
      841 tgttttatta ttggatgaac cgaccaatga tttggacata gcaacattga cgattttgga
      901 agattatttt agaagttttc caggtgctgt gatcactgtt tcccatgacc gttatttctt
      961 ggacaaggta gcagataagt tattagtatt ccaaggaaac ggaaaacaag aactttatta
     1021 cggaaacatg tccagttatt tactgaagca aaaagagacc cagcagcctg ctgaaaaagc
     1081 gaaaccaaaa atacagagca aggaacctgc tgaaaagaag aaattatctt acatggaaca
     1141 aaaagaatgg gaaacgattg aagatgagat tgcagaactt gaagaaaaaa tctcattgct
     1201 ccaagaagag atgaatcatc aaggagataa tttcactcga ttacaagaat tgcaaaacga
     1261 cgtatctgaa acagaagcac aacttgaaga aaaaatggca cggtgggaat acttgagtga
     1321 atgggtagaa gattaggagg aaaaaggatg gaacaagcgt atcttgactt aggaagaaaa
     1381 ttgttggaag aagggcatcg taaagaagac agaacaggca caggcacatt aagcttattc
     1441 ggataccaaa tgcggtttga tctgcaaaaa gggtttcctt tgttaacaac gaaacgagta
     1501 ccttttggat tgatcaaaag cgaattgctt tggtttttaa aaggagatac gaatatccgc
     1561 tatctattac aaaataaaaa ccatatttgg gatgaatggg catttgaacg ctacatcaaa
     1621 agtccagact ataatggacc agatatgact gattttggcc atagatgttt gacagatcct
     1681 gaattcaatg aagaatataa aaagcagagc aagctttttt gcgagaagat cctaaccgat
     1741 gattcctttg ctgaaacata cggcgatctt ggacacatct acggctatca atggcgtcac
     1801 tgggaaacaa aagacggtgg ctttatcgat caaatcaaac aagtgatcga agcaatcaaa
     1861 aagactcctg attctagaag attgatcgtt tctgcatgga atccggaaga tgttcctagc
     1921 atggctttgc ctccttgtca tacgatgttt caattttatg ttcaagaggg acggctaagc
     1981 tgccaattgt atcaacgcag tggggatgtg tttctaggcg ttccgttcaa cattgcaagt
     2041 tatgcgttgc tgactcacct aattgcacat gaaacaggtt tagcggttgg cgaatttgtt
     2101 cacacattag gagatgctca tctttatatc aatcatatcg aacaaatgaa agaacaattg
     2161 agccgagaaa tacgcgcatt tccgacttta gttttgaatc aggaaaagca gtcagtattt
     2221 gattttgaga tggaagatat ccaattagaa ggatatgacc cacatccggc gatcaaagct
     2281 cctatcgctg tttagaatca gaaaggatga gaaaatgtta gctgcaattt gggcacagga
     2341 tgaaaacgga ttgattggaa aagaagaccg tcttccttgg cgtttgccaa atgatctgaa
     2401 attcttcaaa caaatgacag aagcaaatac actagtgatg ggacgaaaaa cattcgaagg
     2461 gatgggtagc cgtccgttgc cgaatcgtca gacgattgtt ttgacgagag attcttccta
     2521 taaagcagaa ggcgtgcatg tcatgcatag tgtggaagac gtgcttgctc tggaaaaaga
     2581 aacagacggt atctttttca tcgctggagg gtcagctgtt tatcaagaat ttctgccatt
     2641 ttgtacgata ttataccgta cagtcatcca tcatgtattc gatggagatg cttattttcc
     2701 acctgttgac tggtctgatt ggtcactgat caatactagt caaggagaaa tggatgaaaa
     2761 aaatcgttat ccgcatcaat ttgaaacgta tcagcgtaat gagaatcctg taaaaagcgg
     2821 gacaggcgaa ttgacgatat aaaaaagcta tggatagact agctgttatt tagagctgtc
     2881 ccatccatag ctttattttt tttattaagt aaagaataaa acagagaagt acataaaacc
     2941 tgcacctaac ataacaaaca aatgccaaac aacatgcata tagcggatag aattcaagct
     3001 ataaaagaat gcgcctatcg tgtaagaaag cccgcctgct accatcaaaa taacaccagt
     3061 cattccaaga gagtgataaa gaggaacagc agcc
//
LOCUS       DADXTG010000357         2233 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.222, whole genome shotgun sequence.
ACCESSION   DADXTG010000357 DADXTG010000000
VERSION     DADXTG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.222"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            92..1576
                     /gene="lsa(E)"
                     /locus_tag="IWI57_002947"
                     /gene_synonym="lsaE"
     CDS             92..1576
                     /gene="lsa(E)"
                     /locus_tag="IWI57_002947"
                     /gene_synonym="lsaE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Lsa(E)"
                     /protein_id="HBC2451007.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LLKEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAAKAMKRAKNIESR
                     HQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDKEVCSNLNFRVNI
                     GDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQDTSYLKGNLSEFA
                     YNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLCESAHLYIWDEPL
                     NYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            1630..>2233
                     /locus_tag="IWI57_002948"
     CDS             1630..>2233
                     /locus_tag="IWI57_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HBC2451008.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSK"
BASE COUNT          804 a          295 c          422 g          712 t
ORIGIN      
        1 ccttgcagag ttggataaca ttatagagta attaatgccg aaaaccgact ttgcttataa
       61 aatttttata atgtaaagga ggaagaaata tatgtcctta ataaatgttt caaatctaac
      121 tttttcatat gaaggaagtt atgacaatat ttttgaaaat gtaagttttc agatagatac
      181 agattggaaa ctcggtttta ttggaagaaa cggacgcggt aaaactactt tcttaaattt
      241 actgcttggc aaatatgcgt attccggcaa tataagttct acagttaagt ttgagtattt
      301 tccttatgat gtggaagata agagtctata tacaattgaa gtaatgaaga gtatttgtac
      361 ggaatttatg gattgggaga tttttcgtga aatatcattg cttgatgttc aagaagatgc
      421 tttatatcgt ccgtttaata cattgtcaaa tggtgagcaa acgaaggtcc ttcttgcagc
      481 tttattcctt acagcgagtt gtttcctgct tattgatgaa cctacaaacc atcttgacat
      541 cgatgcacgt aatgtagtgc aaaactattt gaaacgcaag aaggggttta ttttggtatc
      601 tcatgataga agcttacttg atcaatgtgt tgaccatata ctatctatca ataaaacgaa
      661 tatcgaaatc caaaagggaa attttacttc ttggtgggag aacaaaacgt tacaagataa
      721 ttttgaactg gcagaaaaca agaaactcct taaagaaata ggaaggttgt cttatgcagc
      781 aaaacgtagt tcaaactggt caaataaagt agaaaaaagt aaatatggaa caacaaattc
      841 tggttcaaaa ctggataagg gttatgttgg acataaggct gcaaaagcga tgaaacgtgc
      901 caaaaatatt gagtcaagac atcaggaagc cgttttacaa aaatcagaac tgctccacaa
      961 cattgaacaa tatgatgact taaaaatttc accacttgaa tttcacaaag agtgcttaat
     1021 agaagcgaat gatttatcat tgtcttatgg agataaagaa gtatgcagta atcttaattt
     1081 cagagtcaat attggtgata gagttgccat tatcggaaaa aatgggagtg gtaagtctag
     1141 tatcctaaaa ttgattaatg gagatgatat taaatttacc ggaaatttta tgctagcaag
     1201 tggactaaaa atttcttata tttcgcaaga tacttcatat ttaaaaggta atctatctga
     1261 atttgcctat aataataaga tcgatgaaac tctatttaaa acgattcttc gtaaactgga
     1321 ttttaataga gagcagtttg ataagaacat ggtggatttt agtgctggtc agaaaaagaa
     1381 agtactaatt gctaaaagcc tttgtgaaag tgcacatttg tatatatggg atgagccatt
     1441 gaactatatt gatatttttt cacgtatcca aattgaaaaa atgattttgg aatattgtcc
     1501 tacactattg tttgtggagc atgatgatgc tttttgcaat aacatttgta cgaaaaatat
     1561 taatttaggt ttgtagagat tttgagttgc cacaataact aaaagatatt tacatgaaag
     1621 ggtgaagaaa tgttaaaaca aaaagaatta attgcaaacg ttaagaatct tactgagtca
     1681 gatgaacgaa ttacagcttg tatgatgtat ggatcgttta ccaaaggaga aggtgaccaa
     1741 tactctgata tagagttcta tatatttttg aaagatagta taacctcgaa ctttgattca
     1801 tccaactggt tgtttgacgt agctccgtac ttgatgcttt ataaaaatga gtacggaaca
     1861 gaggtagtta tttttgataa tcttatacgt ggggaatttc atttcctttc tgaaaaagat
     1921 atgaacataa tcccctcgtt taaagattca ggttatattc ctgatacgaa ggctatgctt
     1981 atttacgatg aaacagggca attagaaaat tatttatcag agataagtgg tgcaagacca
     2041 aatagactta ctgaagaaaa tgctaatttt ttgttgtgta atttctctaa tctatggttg
     2101 atgggaatca acgttctaaa aagaggagaa tatgctcgtt cattagaact cttatcacaa
     2161 cttcaaaaaa atacactaca acttatacgt atggcagaaa aaaatgctga taattggcta
     2221 aacatgagta aaa
//
LOCUS       DADXTG010000358         1839 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.239, whole genome shotgun sequence.
ACCESSION   DADXTG010000358 DADXTG010000000
VERSION     DADXTG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.239"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWI57_002949"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWI57_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWI57_002950"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWI57_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBC2451009.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1594..1677
                     /locus_tag="IWI57_002951"
                     /pseudo
     CDS             1594..1677
                     /locus_tag="IWI57_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1726..1809
                     /locus_tag="IWI57_002952"
     CDS             1726..1809
                     /locus_tag="IWI57_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBC2451010.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
BASE COUNT          589 a          290 c          416 g          544 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1381 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1441 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1501 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1561 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1621 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1681 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1741 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1801 gataaataaa tacgttagat taattcctac cagtgacta
//
LOCUS       DADXTG010000359         1268 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.264, whole genome shotgun sequence.
ACCESSION   DADXTG010000359 DADXTG010000000
VERSION     DADXTG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.264"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            43..1083
                     /locus_tag="IWI57_002953"
     CDS             43..1083
                     /locus_tag="IWI57_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBC2451011.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            1202..>1268
                     /locus_tag="IWI57_002954"
     CDS             1202..>1268
                     /locus_tag="IWI57_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBC2451012.1"
                     /translation="MCPVCGNKTRLKIREDTELKKF"
BASE COUNT          434 a          185 c          257 g          392 t
ORIGIN      
        1 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
       61 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
      121 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
      181 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
      241 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
      301 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
      361 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
      421 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
      481 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
      541 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
      601 gggatacgct atggttgtga acaaggattg tatggttgga atgtgacgga ctgtaaaatc
      661 tgttttaagt atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt
      721 gctcctattg tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat
      781 cttagtttta aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct
      841 aaatattgtg cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga
      901 gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga
      961 cgtagtgttt gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc
     1021 cagccccgtc gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact
     1081 tagtgtattt tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt
     1141 taataaagat ttgaattaaa gtgtaaagga ggagatagtt attataaact acaagtggat
     1201 attgtgtcct gtatgtggaa ataaaacacg attaaagata agggaagata ctgaattaaa
     1261 aaaattcc
//
LOCUS       DADXTG010000360          924 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.296, whole genome shotgun sequence.
ACCESSION   DADXTG010000360 DADXTG010000000
VERSION     DADXTG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.296"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            95..832
                     /gene="erm(B)"
                     /locus_tag="IWI57_002955"
     CDS             95..832
                     /gene="erm(B)"
                     /locus_tag="IWI57_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBC2451013.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          345 a          156 c          143 g          280 t
ORIGIN      
        1 atcttatgac tttttaaaca gataactaaa attacaaaca aatcgtttaa cttctgtatt
       61 tgtttataga tgtaatcact tcaggagtga taacatgaac aaaaatataa aatattctca
      121 aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga
      181 aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa
      241 aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga
      301 aaaattaaaa ctgaatactc gtgtcacttt aattcaccaa gatattctac agtttcaatt
      361 ccctaacaaa cagaggtata aaattgttgg gaatattcct taccatttaa gcacacaaat
      421 tattaaaaaa gtggtttttg aaagccgtgc gtctgacatc tatctgattg ttgaagaagg
      481 attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt
      541 ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa
      601 cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct
      661 atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa
      721 tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtaccg ttacttatga
      781 gcaagtattg tctattttta atagttatct attatttaac gggaggaaat aattctatga
      841 gtcgcttttg taaatttgga aagttacacg ttactaaagg gaatgtagat aaattattag
      901 gtatagggca cctctaataa ctac
//
LOCUS       DADXTG010000361          787 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.308, whole genome shotgun sequence.
ACCESSION   DADXTG010000361 DADXTG010000000
VERSION     DADXTG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.308"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            174..722
                     /gene="aac(6')"
                     /locus_tag="IWI57_002956"
     CDS             174..722
                     /gene="aac(6')"
                     /locus_tag="IWI57_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBC2451014.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          282 a          116 c          171 g          218 t
ORIGIN      
        1 ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta
       61 agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca
      121 tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa
      181 tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac
      241 tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc
      301 cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa
      361 tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac
      421 gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta gcttccagag
      481 gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa
      541 cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc
      601 cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg
      661 gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat
      721 aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa
      781 attactt
//
LOCUS       DADXTG010000362          599 bp    DNA     linear   BCT 19-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate E51419_2015
            SAMN12639603-rid9883953.guided.326, whole genome shotgun sequence.
ACCESSION   DADXTG010000362 DADXTG010000000
VERSION     DADXTG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639603
            Sequence Read Archive: SRR10030870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742175.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742175.1
            Genome Coverage       :: 68x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/16/2021 15:38:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,957
            CDSs (total)                      :: 2,893
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 21 of 56
            Pseudo Genes (incomplete)         :: 30 of 56
            Pseudo Genes (internal stop)      :: 14 of 56
            Pseudo Genes (multiple problems)  :: 8 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639603-rid9883953.guided.326"
                     /isolate="E51419_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..542
                     /gene="dfrF"
                     /locus_tag="IWI57_002957"
     CDS             69..542
                     /gene="dfrF"
                     /locus_tag="IWI57_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBC2451015.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
BASE COUNT          217 a           55 c          138 g          189 t
ORIGIN      
        1 agtttgttaa gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga
       61 tgaaatagat gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg
      121 gtaatatacc atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta
      181 atgtggttat tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa
      241 tgaatattgt tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat
      301 cattagaaga tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg
      361 gactatttaa ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa
      421 atattgaaga tggagataca ttctttccag aatttgatat caatgatttt gaagttttga
      481 taggggaaac acttggtgag gaagtgaaat atacgagaac attttatgta aggaaaaaat
      541 gaattgagta aattttggat ttagggtgtt ttcataaata tattgctttt tatgcatat
//
